LOCUS       DAMQYT010000001        62589 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMQYT010000001 DAMQYT010000000
VERSION     DAMQYT010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 62589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62589
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.001"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(77..922)
                     /locus_tag="PGA33_000001"
     CDS             complement(77..922)
                     /locus_tag="PGA33_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDG6471843.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQEDFVRIIEQLN
                     RRYGK"
     misc_binding    1151..1520
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1586..2689
                     /locus_tag="PGA33_000002"
     CDS             1586..2689
                     /locus_tag="PGA33_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDG6471844.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     gene            2686..3846
                     /locus_tag="PGA33_000003"
     CDS             2686..3846
                     /locus_tag="PGA33_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HDG6471845.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISD
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            3815..5656
                     /locus_tag="PGA33_000004"
     CDS             3815..5656
                     /locus_tag="PGA33_000004"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HDG6471846.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAFLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLYGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            5653..7881
                     /gene="metE"
                     /locus_tag="PGA33_000005"
     CDS             5653..7881
                     /gene="metE"
                     /locus_tag="PGA33_000005"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDG6471847.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            7924..8676
                     /locus_tag="PGA33_000006"
     CDS             7924..8676
                     /locus_tag="PGA33_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDG6471848.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(8759..9940)
                     /locus_tag="PGA33_000007"
     CDS             complement(8759..9940)
                     /locus_tag="PGA33_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDG6471849.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            10479..11612
                     /locus_tag="PGA33_000008"
     CDS             10479..11612
                     /locus_tag="PGA33_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HDG6471850.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLTSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISYSLGIL
                     VMSYIVSLLFKGHDHVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            complement(11979..12608)
                     /locus_tag="PGA33_000009"
     CDS             complement(11979..12608)
                     /locus_tag="PGA33_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDG6471851.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMICASILLLISFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIAYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(12608..13450)
                     /locus_tag="PGA33_000010"
     CDS             complement(12608..13450)
                     /locus_tag="PGA33_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6471852.1"
                     /translation="MTTLLNVDSVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSITFFGEEHTVDDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYMTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMEILEDFVAQGGGILISSHISEDIEHLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTEDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            complement(13475..14188)
                     /locus_tag="PGA33_000011"
     CDS             complement(13475..14188)
                     /locus_tag="PGA33_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HDG6471853.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGFGIENLTFATYNNVV
                     VISIVATIIIILVEAIVLMNQRRALKYKQLVDKEVDIDATDQYELLANRYVLNGSILS
                     ILQTVIAFLVLLIFVVGHAAANAILFFLIPFFASAIFNTQFTLFNRRFDDRIPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIVVIGLYSITTGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            complement(14185..14388)
                     /locus_tag="PGA33_000012"
     CDS             complement(14185..14388)
                     /locus_tag="PGA33_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDG6471854.1"
                     /translation="MRNRLKELRARDGLNQTQLAKQAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEA"
     gene            14629..15018
                     /locus_tag="PGA33_000013"
     CDS             14629..15018
                     /locus_tag="PGA33_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HDG6471855.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAQKSNKTIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLQDQAVISELIPQ"
     gene            15118..15342
                     /locus_tag="PGA33_000014"
     CDS             15118..15342
                     /locus_tag="PGA33_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HDG6471856.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            15359..16060
                     /gene="tatC"
                     /locus_tag="PGA33_000015"
     CDS             15359..16060
                     /gene="tatC"
                     /locus_tag="PGA33_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDG6471857.1"
                     /translation="MVHFSELRHRLVKILLSFVITVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSFFS
                     VLLFCAGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLIELIRWLFTFGLLFQL
                     PILFMGLAKFGLIDTTSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFS
                     MFIVKFTCRGKPPTH"
     gene            complement(16152..17864)
                     /locus_tag="PGA33_000016"
     CDS             complement(16152..17864)
                     /locus_tag="PGA33_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDG6471858.1"
                     /translation="MKHYLTKFVAMLITAAMVCSFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSESNAVKSDVRKLEDAKANDNQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSNTSANDQYHVSQLNDALEKAIKAIDDNQLSDADAALTHFIETWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVKLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQGAYIMVVAILIF
                     KFKK"
     gene            complement(17845..19074)
                     /gene="efeB"
                     /locus_tag="PGA33_000017"
     CDS             complement(17845..19074)
                     /gene="efeB"
                     /locus_tag="PGA33_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDG6471859.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKNNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            complement(19071..19925)
                     /locus_tag="PGA33_000018"
     CDS             complement(19071..19925)
                     /locus_tag="PGA33_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471860.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDIIPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            complement(20193..20738)
                     /locus_tag="PGA33_000019"
     CDS             complement(20193..20738)
                     /locus_tag="PGA33_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6471861.1"
                     /translation="MFGMKVNEQITLKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFADGNGFQCGIWYEGTLVGVIGLHEINHMHRKTSLGYYLDKE
                     FEGHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYDQK"
     gene            complement(20818..21102)
                     /locus_tag="PGA33_000020"
     CDS             complement(20818..21102)
                     /locus_tag="PGA33_000020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471862.1"
                     /translation="MDINQSNRHYNAYWRGPNKEADEKSAYNNVQVGDGPQHREIGSV
                     ISTDNASWRGPNIENFEKKFYKQCKLAGPQHREFRKEILQAMQVGEGQQI"
     gene            21212..22207
                     /locus_tag="PGA33_000021"
     CDS             21212..22207
                     /locus_tag="PGA33_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDG6471863.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSKLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            complement(22267..22833)
                     /locus_tag="PGA33_000022"
     CDS             complement(22267..22833)
                     /locus_tag="PGA33_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDG6471864.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQSTMRTAKVQQQVFEEENNQYDF"
     gene            complement(22847..23908)
                     /locus_tag="PGA33_000023"
     CDS             complement(22847..23908)
                     /locus_tag="PGA33_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDG6471865.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNILPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            complement(23922..24848)
                     /locus_tag="PGA33_000024"
     CDS             complement(23922..24848)
                     /locus_tag="PGA33_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDG6471866.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLENNGI
                     KSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAVNTPQMSDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPVEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            25156..26514
                     /gene="glpT"
                     /locus_tag="PGA33_000025"
     CDS             25156..26514
                     /gene="glpT"
                     /locus_tag="PGA33_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDG6471867.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            complement(26597..27037)
                     /locus_tag="PGA33_000026"
     CDS             complement(26597..27037)
                     /locus_tag="PGA33_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HDG6471868.1"
                     /translation="MCKSKILLKNIFSEESEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKSGYFVTCWTKDEDNYNRPYKIEEFADYLIVAVIDDELNGYFLFPRELLV
                     EKGILASSKYQGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(27141..28496)
                     /gene="mepA"
                     /locus_tag="PGA33_000027"
     CDS             complement(27141..28496)
                     /gene="mepA"
                     /locus_tag="PGA33_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HDG6471869.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMVSMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMSAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMASLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            complement(28603..29022)
                     /gene="mepR"
                     /locus_tag="PGA33_000028"
     CDS             complement(28603..29022)
                     /gene="mepR"
                     /locus_tag="PGA33_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HDG6471870.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            29234..31189
                     /locus_tag="PGA33_000029"
     CDS             29234..31189
                     /locus_tag="PGA33_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDG6471871.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEA"
     gene            31194..31637
                     /locus_tag="PGA33_000030"
     CDS             31194..31637
                     /locus_tag="PGA33_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDG6471872.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            31639..31923
                     /locus_tag="PGA33_000031"
     CDS             31639..31923
                     /locus_tag="PGA33_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDG6471873.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            31938..33284
                     /locus_tag="PGA33_000032"
     CDS             31938..33284
                     /locus_tag="PGA33_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDG6471874.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAIAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLAVGIVFGNAVKYMGLFGAILFIIIVGATTILLKGRQKEQQ"
     gene            complement(33377..34402)
                     /locus_tag="PGA33_000033"
     CDS             complement(33377..34402)
                     /locus_tag="PGA33_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HDG6471875.1"
                     /translation="MNNKVLATGGTGFVGMRIISRLLEQGYDVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAVDS
                     MAKYHLI"
     gene            complement(34717..35739)
                     /locus_tag="PGA33_000034"
     CDS             complement(34717..35739)
                     /locus_tag="PGA33_000034"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDG6471876.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGID
                     DIKEAKRYELTDADWEAIDELADKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIYGDFFGQGDIKDVEEALQGTKMTREDLTHQLKQLDIVYYFSNVTVEAL
                     VDMILS"
     gene            complement(35717..36661)
                     /locus_tag="PGA33_000035"
     CDS             complement(35717..36661)
                     /locus_tag="PGA33_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HDG6471877.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDVADYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLHRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITAALRNDSTTKNNNIGETEDVLNRTD"
     gene            complement(36651..37451)
                     /locus_tag="PGA33_000036"
     CDS             complement(36651..37451)
                     /locus_tag="PGA33_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDG6471878.1"
                     /translation="METLKSNKARLEYLINDMHRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LSAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFNRDAE"
     gene            complement(37452..37784)
                     /locus_tag="PGA33_000037"
     CDS             complement(37452..37784)
                     /locus_tag="PGA33_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDG6471879.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            complement(37818..38819)
                     /locus_tag="PGA33_000038"
     CDS             complement(37818..38819)
                     /locus_tag="PGA33_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDG6471880.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEDFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEKVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            complement(38933..39025)
                     /locus_tag="PGA33_000039"
     CDS             complement(38933..39025)
                     /locus_tag="PGA33_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471881.1"
                     /translation="MESIFKIKLMNGICRSENMNMKKKNKGEKI"
     gene            39121..40320
                     /locus_tag="PGA33_000040"
     CDS             39121..40320
                     /locus_tag="PGA33_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDG6471882.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            40393..41220
                     /locus_tag="PGA33_000041"
     CDS             40393..41220
                     /locus_tag="PGA33_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDG6471883.1"
                     /translation="MTEIKDKVITKDAFALPYTIIKAKNQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSVNETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTNSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMM"
     gene            complement(41462..43534)
                     /gene="lip2"
                     /locus_tag="PGA33_000042"
     CDS             complement(41462..43534)
                     /gene="lip2"
                     /locus_tag="PGA33_000042"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HDG6471884.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTSTN
                     AAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQSTQ
                     NSKTIRMQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQ
                     SNDVDKSQPSIPAQKVIPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTH
                     QPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAP
                     KTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKF
                     KVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKT
                     YKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGG
                     HNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFK
                     QLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSS
                     HTGPLGYENPDLGTFFLMATTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVTN
                     DEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATES
                     KGTQLKAS"
     gene            43952..45265
                     /locus_tag="PGA33_000043"
     CDS             43952..45265
                     /locus_tag="PGA33_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDG6471885.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPISNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQALLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
     gene            complement(45392..46060)
                     /locus_tag="PGA33_000044"
     CDS             complement(45392..46060)
                     /locus_tag="PGA33_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDG6471886.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     IMED"
     gene            46200..47000
                     /locus_tag="PGA33_000045"
     CDS             46200..47000
                     /locus_tag="PGA33_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDG6471887.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            complement(47278..48138)
                     /locus_tag="PGA33_000046"
     CDS             complement(47278..48138)
                     /locus_tag="PGA33_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDG6471888.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIAGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            48296..49177
                     /locus_tag="PGA33_000047"
     CDS             48296..49177
                     /locus_tag="PGA33_000047"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDG6471889.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            49217..50749
                     /locus_tag="PGA33_000048"
     CDS             49217..50749
                     /locus_tag="PGA33_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDG6471890.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVVNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(50854..52074)
                     /locus_tag="PGA33_000049"
     CDS             complement(50854..52074)
                     /locus_tag="PGA33_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDG6471891.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKKIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINISKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     TDVEIDNLTKSTETKTLNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(52085..53008)
                     /locus_tag="PGA33_000050"
     CDS             complement(52085..53008)
                     /locus_tag="PGA33_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDG6471892.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVIAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            complement(52983..54101)
                     /locus_tag="PGA33_000051"
     CDS             complement(52983..54101)
                     /locus_tag="PGA33_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDG6471893.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKNLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            complement(54444..55475)
                     /locus_tag="PGA33_000052"
     CDS             complement(54444..55475)
                     /locus_tag="PGA33_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDG6471894.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPTTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            complement(55683..56360)
                     /locus_tag="PGA33_000053"
     CDS             complement(55683..56360)
                     /locus_tag="PGA33_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6471895.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKQRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            complement(56373..57422)
                     /locus_tag="PGA33_000054"
     CDS             complement(56373..57422)
                     /locus_tag="PGA33_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDG6471896.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASSVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTVILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            complement(57671..58561)
                     /locus_tag="PGA33_000055"
     CDS             complement(57671..58561)
                     /locus_tag="PGA33_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDG6471897.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            59145..60452
                     /gene="brnQ"
                     /locus_tag="PGA33_000056"
     CDS             59145..60452
                     /gene="brnQ"
                     /locus_tag="PGA33_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDG6471898.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRLFGSFGNLV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT"
     gene            60689..61513
                     /locus_tag="PGA33_000057"
     CDS             60689..61513
                     /locus_tag="PGA33_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDG6471899.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(61729..62127)
                     /locus_tag="PGA33_000058"
     CDS             complement(61729..62127)
                     /locus_tag="PGA33_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDG6471900.1"
                     /translation="MEKSIKIMTIIGIVVQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLSLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLISGIKLLTNNKPKDEISDLS"
     gene            complement(62280..>62589)
                     /locus_tag="PGA33_000059"
     CDS             complement(62280..>62589)
                     /locus_tag="PGA33_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HDG6471901.1"
                     /translation="EKYQKDIDKVYKEQNQMNKIASKVQNTIKTDIKQEDSNTHVYKD
                     GKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREIDPIKYMKDHKADYEDENVEVEKD
                     "
BASE COUNT        19606 a        11985 c         9570 g        21428 t
ORIGIN      
        1 tatatctaga atcaaatgtt cactgattac aatttattta cacttgcaca aagagttatt
       61 atataaaatt gtgtaactat ttaccatacc tacgatttaa ttgttcaatt attctcacaa
      121 aatcttcttg tgaatcaaac tcaaatgaga ttttaccaac cgattttttt attgatatat
      181 ctactttggt accatactgt tctcgcaact gtcgttcttg ctgctttata aatttaggct
      241 tagttatatc tactttgtct gtttccgact ttgacgaaac attttttaat tcattaacat
      301 ggttttctaa atatctgaca ctccactttt ctttaacaac ccgtttcgct aacctaagca
      361 tttgttgttc atctttaatt gccaataacg ttcgtccatg tgcacttgtc agtcgcccat
      421 cttttaccat gtcagcaatc tttttcggca aatgtaataa cctcaacata ttcgctatat
      481 acgggcgcga cttactcaat cgtttcgcta cttcttgttg tgtaattttc aaatctgtca
      541 tcaaacgttg ataactttca gcttcttcaa tcgcatttaa gtcttctcgt tgtaaatttt
      601 cgatgaccgc cagttccatc atatcttcat ctgttaaatc tttgataatc gctgatacgt
      661 attttagacc agcaattttc gaagctctaa accttctttc accaactaca atgtaataac
      721 cttgaactgt ttttctaagc acaattggtt gcaaaattcc atattgctta attgaatctg
      781 ccaaatcatt taaatgattt tcatcgaaag tttttcgggg ctgatacgga ttcggtttga
      841 tatccgatat atctatttgc ttaatttgtt catcttttgt tttagtaata cgttgatctt
      901 cactttttga caattcactc acatcacttt ttatttgata acaacagtta aatatatttt
      961 atgtattcta aatgagtcag acaaccatta accacacttt atttaaaaaa taattcattt
     1021 ttcaacttca agataccaat tacaatttca caaacaaatt aacttttaaa tcaaaatttt
     1081 ctgaataatt gttgacaaat ctttttttac tctgtaagct aattaatatc ttataacagt
     1141 ttaatgaaac gtaaacacaa taaagaggaa agtaaaacac accctgctta tacagagagt
     1201 ctttagtagc tgagagaaga ttttgaaagc gtgtttgaaa atggccttgg agtgttgatg
     1261 ccaatatgag gtgtctacgg gttcgcccgt tatagcgata cagtattaac attgatgtta
     1321 aatggcgtac tggattcttt acgcacgatt ttttgttaat aagtatggga tagcacatta
     1381 ctatatcctt acttactgac tttaattgtg ataattgttc agtaagcata tttactttta
     1441 atgcgtactg aataaggtta tttcagcgat ggaataacaa ataaaggtgg taccgcgaaa
     1501 cataagcttt cgtccttttt atccgattca ttcgggtacg aaggacggaa gcttttttta
     1561 ttttttctta taggagggtc ttaatatgaa ggatacacag ttagcccaaa tcacattaac
     1621 cgatgattca accggtgcta tagcgaatcc aatccattta tctactgcct acaagcatcc
     1681 aaaactagga caatcgacag gttttgatta tacacgtact aaaaatccta cacgctcaac
     1741 atttgaaacc tgttttgcca aacttgagca tggtattgca tcattcgcta catcaagtgg
     1801 aatgtcagcc attcaattaa tatgtaatct atttaaacct catgatgaaa ttttagtttc
     1861 attcgattta tatggtggca catttagatt atttgaattt tacgagcaac aatacgatat
     1921 caaatttaag tacgttgatt ttacagatta tgaacaagtt gaaaaagaaa tcactgataa
     1981 aacagttgca ttattcattg aaccaatatc taacccacaa atgattgcta ttgatgtaaa
     2041 gccatactat caactttgta aagctaaagg cttattgtca attatcgaca atactttttt
     2101 aacaccttat ctttcaacac cactagcaga aggtgctgat atagtcttac attcagccac
     2161 gaaatatatt ggcggacata acgatgtact agcaggtgtc gtaaccgtca aagatgaatc
     2221 actcgcgcaa cagttgtttg attttcacaa catgactggc gcaacacttt caccaataga
     2281 tagttatttg ttgttacgtg gacttaaaac tttgcattta cgcattgagc gtgcgcaatc
     2341 aaacgctaga aaacttgcta aaaaatgtca gtcacttcaa gcaattgacg aagtactata
     2401 tagcgggcaa actggcatgc ttagtttaag acttaacaag gcctatagcg tcgctaaatt
     2461 attagaaaat ttagacattt gcatttttgc agaaagttta ggaggtactg aaacattagt
     2521 gaccttccct tacacccaaa cacatgttga tatgccagat gctgaaaaag ataaacgtgg
     2581 cattgatgag tatttaatcc gcttatcact aggtgttgaa aattatgaag acatcgaacg
     2641 cgatatcatc caagcattag ataaagctca gattggagag attgtatgac actttcaaaa
     2701 gagacagaag tgatattcga ttggcgtaga ggtgtggaat atcattcagc taacccacca
     2761 ctctatgatt cttcaacatt ccatcaaaca agtcttggtg gcgatgttaa atatgattat
     2821 gcacgaagtg gcaaccctaa ccgtgaactt ttagaagaga agttagcacg attagaacag
     2881 ggaaaattcg cttttgcttt tgcatcaggt attgctgcta tttcagcagt acttttgact
     2941 ttcaaatctg gtgatcatgt catcttaccc gatgatgtat atggcggtac ttttcgcctc
     3001 actgagcaaa ttttgaatcg atttaatatt gaatttacaa ccgtagatac tacaaagctc
     3061 gaacaaatcg agggtgccat tcaatcaaac acaaaattaa tttatatcga aacaccatcg
     3121 aatccctgtt ttaaaattac tgatatcaaa gctgtttcta aaatagccga aaagcatgaa
     3181 ctactggtag ctgtggacaa tacatttatg acaccgttag gtcaatcacc tttattactt
     3241 ggcgctgata ttgtcattca tagtgctaca aaatttttaa gtggacatag cgatttaatt
     3301 gctggtgctg tcataactaa taatgaggca attagtgacg ctctttattt aatacaaaat
     3361 ggtacaggca atatgttatc tgctcaggat agctggacac ttgctaaaca tttaaaaact
     3421 tttccaatca gatttaaaca atctgtcgaa aacgctcaaa aaatcgtgtc atttttaata
     3481 aagcaagatg aaatttcaga agtttattat ccgggactca ctactgctca tttagaacaa
     3541 gctaaaaatg gcggtgccgt tattggcttt cgtttagctg atgagtctaa agcacaacaa
     3601 tttgtcgatg cactgacatt accactcgtt tcagtgagtc tgggcggtgt tgaaacgatc
     3661 ctttcacatc cagcaacaat gtctcacgct gcactacctg aagaagtgag acaagaacgt
     3721 ggtatcactt tcggtctatt ccgattaagt gttggtctcg aagatcctga tgaactcatt
     3781 gcagacatca aatacgcatt aaaggaggca ttcaatgagt caattcctca cacaattgaa
     3841 agataatgtt ttagtagctg atggcgctat aggaaccatt ttatactctg aaggattaga
     3901 cacctgtcca gaagcatata atcttagcca tccagataaa gttgaacgca tccatcgttc
     3961 atatattgaa gccggtgctg atgtcattca aaccaatact tatggtgcaa attttgaaaa
     4021 gttaaaacga ttcggtcttg aagataaagt taaagcaata catcaagccg ccgttcgcat
     4081 cgcaaaaaaa gcagcaaata aagatacgta tatattaggc acagttggtg ggtttagagg
     4141 tatcaaacaa gaggatatca gcttacaaac tattctttat catactgaaa ttcaaataga
     4201 caccttaatt gaagaaggcg ttgacgcgct acttttcgaa acgtattacg acctagaaga
     4261 gttaacaaat gtcatttcac gaacgagaaa gaaatacgac attccaatca ttgctcaatt
     4321 aaccgcttca aacacaaatt acttagttaa tggtcaggca atcaatgaag gattaaaaca
     4381 actcgttcaa tgtggtgcaa acatcgtggg actcaattgt catcatggcc cgcaccatat
     4441 gcaagagtct ttcacacata ttgaattacc agagcacgca ttcttatctt gttatccaaa
     4501 tgccagctta ttagatattg aaaatagtga atttaagtat agtgacaatg cacaatattt
     4561 cggtcaagtt gctcaaaatc taattcgcga aggtgttcgt ttaattggtg gttgctgtgg
     4621 tacaacgcca gagcacatca aatttattaa agaatctatt cagacactta aacctgttaa
     4681 tgacaaaaaa gtgattccga taccaacgaa agcacttttc aatccatctc aaaataaagt
     4741 tagacaatca ttaacatcta aggttcaaga acgtccaacc gttattatcg aattggatac
     4801 accgaaacat ttagacacgg atagattttt tgaaaatatc gctaaacttg ataaagctaa
     4861 tgtagatgcg gtaacactcg cagataattc attggcaact gtcagaatta gcaatattgc
     4921 tgctgctagc ttaattaagc aatattacaa tattgaacca ctcgtacata ttacatgtcg
     4981 agaccgaaac ttaatcggct tgcagtccca tttacttgga ttatcgctca ttggcgttaa
     5041 cgaaatatta gccataactg gtgatccttc aaaagttggt cacttaccag gtgcaaccaa
     5101 tgtctatgat gttaattcta aaggattaac tgaactcgct ctaagattta atcaaggtat
     5161 taacactgac ggtgatgcgc tgaagaaacg tacacacttc aacatcgctg gcgcctttaa
     5221 ccctaatgtt cgtaaattat atggtgccgt caaaagatta gagaaaaaga tagaaagcgg
     5281 aatgtcttat tttataacac aacccgtgta cagcaaagag aaaatcattg aaatttacca
     5341 tgccactaag cacttgaaca aaccattttt cataggcatt atgcctatcg caagttacaa
     5401 aaacgcactc tttttgcata atgaagtgcc aggaatcaag atgtcagatg aaattttaca
     5461 acaatttgaa gcagttaaag atgataaagc caaaacacga gaactaagtc ttaagctttc
     5521 aaaggattta atcgatactg ttcatgaata ttttaatggt ttatacatta tcacaccgtt
     5581 tcaaaatgtc gaagattcat tagaacttgc agcatactca aaatctatta ctgctcacaa
     5641 ggaggcaata ttatgacaac aattaaaaca tcaaacttag gattcccaag attaggtaga
     5701 aaaagagaat ggaaaaaagc catcgaaagt tattgggcaa agaagatttc taaagaagaa
     5761 ttagatcaaa cattaactga tctacataaa gaaaatttat tattacaaaa gtactatcac
     5821 ttagatagca tcccagttgg tgatttctct ttatatgacc atatattaga tacgtcatta
     5881 ttattcaaca tcatccctga acgtttccaa ggaagaacta ttgatgatga tttattattt
     5941 gatattgctc gcggtaataa agaccatgtt gcaagtgcac ttataaaatg gtttaacact
     6001 aactatcatt acatcgtacc tgaatgggac aatgttgagc ctaaagtgag tcgcaatgta
     6061 ttattagatc gctttaaata tgcccaatct ttaaacgtta acgctcaccc tgtcattgtt
     6121 ggtccgatta cttttgttaa attatcaaaa ggcggtcacc aaacttttga agaaaaagtt
     6181 aaaacgttat taccacttta taaagaagtg tttgaatcat taattgatgc aggtgctgag
     6241 tacattcaag ttgatgagcc aatcttagtt acagacgaca gcgaaagcta tgaaaatatt
     6301 acacgtgaag cttatgacta tttcgaaaaa gctggtgttg ctaaaaaatt agtcattcaa
     6361 acatactttg aacgtgcaca tcttaaattc ttaagttctt tacctgttgg tggtataggt
     6421 ttagactttg tccatgataa tggctataac cttaaacaaa ttgaagctgg agattttgat
     6481 aaatcaaaaa cattatacgc tggaattatt gatggtcgta atgtatgggc aagtgacatt
     6541 gaagctaaaa aagtcttaat cgataaattg ttagcacaca ctaatgaact tgtcattcaa
     6601 ccatcatctt cattattaca tgttccagta tctttagatg atgaaacatt agatacaagt
     6661 gttggcgaag gcttaagctt tgcaactgaa aaattagacg aattagatgc attgcgtcgc
     6721 ctatttaatc aaaacgatag tgttaaatat gataaattaa aagcacgtta tgagcgtttc
     6781 caaaatcaat cattcaaaaa cttagattat gatttcgaaa gcgtgcgtac ttcaagacaa
     6841 tcaccattcg cgcaacgtat tgaacaacaa caaaaacgtt tgaacttacc ggatttacca
     6901 acaacaacta ttggatcatt cccacaaagc cgagaagttc gaaaataccg tgcagattgg
     6961 aagaacaaac gcattacaga cgaagcatat gaaacattct taaaaaatga aattgctcga
     7021 tggattaaaa ttcaagaaga cattggctta gatgtattag ttcacggtga atttgaacgt
     7081 aatgacatgg ttgaattctt cggagaaaaa ttacaaggtt tcttagtaac taaattcggt
     7141 tgggtgcaat catatggttc acgtgccgta aaaccaccaa tcatttatgg tgatgtaaaa
     7201 tggacagcgc ctttaactgt tgatgaaaca gtttatgcac aaagcttaac agataaacca
     7261 gttaaaggta tgttaactgg acctgtaaca attctaaact ggtcatttga acgtgttgat
     7321 ttaccacgta aagtcgttca agatcaaatt gctttagcaa tcaacgaaga agtattagca
     7381 cttgaagctg caggaatcaa agttatccaa gttgacgaac ctgcattacg tgaaggctta
     7441 ccattacgct ctgaatatca cgaacaatat cttaaagatg ctgttttatc atttaaactt
     7501 gcaacgtctt cagttcgtga tgaaactcaa atccatacac atatgtgtta ttctcaattc
     7561 ggtcaaatca ttcatgctat tcatgactta gatgctgatg ttatttcaat tgaaacatct
     7621 cgtagccatg gtgatttaat taaagacttt gaagatatta attatgattt aggtattggt
     7681 ctaggtgtat atgatattca tagtccacgt attccaacaa aagaagaaat tactacagca
     7741 atcaatcgtt cactacaaca aatcgaccgc tcattattct gggtaaaccc tgactgtggt
     7801 ttaaaaacgc gaaaagaaga agaagttaaa gatgcattga ctgtgcttgt gaatgctgtt
     7861 aaagctaaac gccaagaata atcgcagcat ttatcatata ataaactaaa aacgggtgat
     7921 tttatgacac aatatccttt atggcaacaa ttaaatactt taaaacaagc aacttgggtt
     7981 gatttaacgc atacatttga tccggaaata ccacgtttta gtgagtttga aaaaggcgaa
     8041 gtttcaactt tattcacggt taaagatcat ggtttctatg tgcaacgttg gaatattgta
     8101 acgcaatatg gtactcacat tgatgcacca attcactttg tcgagaataa acgttattta
     8161 gaagacattg atttaaaaga attggtattg ccgttaattg tattagactt ttcaacagaa
     8221 gtagcgaaca acaatgattt catcgtaaca cgtgcgcata tagaagcatg ggaaaaagag
     8281 catggtacta ttgaacctgg cacttttgtt gcacttcgta ccgattggtc aaaacgttgg
     8341 cctaatattg aaaagtttga aaacaaagat gcgaacggac aacaacatgc accgggttgg
     8401 ggattagatg ctttgaagta cttaattgaa gaacgtcgtg ttgaagctgt tggtcatgaa
     8461 acattcgata ccgatgcatc tgtggacgtt gtaaaaaatg gtgatttagt tggcgaacgt
     8521 tatattcttg gtcaggataa ataccaagtc gagttattaa ctaatttaga tcaattacca
     8581 actcgtggcg ccatcattta cgctatcagc cctaaaccaa aagatgcacc tgggttccct
     8641 gttagagcat ttgcaattaa accatcaaat gactaatatt acccaataaa tccataaaca
     8701 aataaaaaca ggttatgcca acatgtaatt aatactgttg tgataacctg ttttcttatt
     8761 atttatactt tgatacaact gcagcgatag cttgaccgcc accaatacac aatgatgtta
     8821 aaccagtttc aacttcatca ttcagttgat gcaataatgt cactaatact ctagcaccag
     8881 atgcaccaat aggatgtcct aatgcaatag cgccaccttt aacattcacc ttttcaggag
     8941 gtaattttaa ttcacgatca acagctaatg attgtgctgc aaatgcttca tttaattcaa
     9001 atacatcaat atcgcttaat tcttttttac tacgtttcaa agccttttct acagcgccaa
     9061 ctggtgcaat acccataata gaaggatcta caccatgact tccaaagcca tcaagcactg
     9121 ccaatggttc gatatttaat tctttagctt tgtcttctga catgactaac atcatcgcag
     9181 caccatcatt gattcctgat gcattacctg ctgtaactgt accgtctttt ttgaaagctg
     9241 gtcttaatcg acttaatttt tcgactgata cattttcacg tacaccttca tccttagtga
     9301 ctacgattgg ttcaccttta cgttgaggaa tcgatactgg aactatttca ctatcaaatt
     9361 caccattttg ctgtgcacgt actgcttttt gttgtgagtt tacagcaaat gtatcttgtt
     9421 cttctcttga aataccatat tgctctacta aattttcagc agtaataccc atatgatatt
     9481 gattaaatac atctgttaaa ccatcatata ccatgctatc aaccattgat tgatgtccca
     9541 ttttaaaacc aaagcgactg ttgttgacaa gcattggtga ttgagacata ttctccatac
     9601 cgccagctag cacgatgtca ttttcaccag tcacaataga ttgatatgct aattgaatcg
     9661 actttaaccc agaaccacat actttattca ccgtaaatgc aggtactgtt tctggcaagc
     9721 cacctttcat agcagcaatt cgtgctggat tttgtccttg tcctgcttgt agtacgttac
     9781 cgatgataac ttcatcaatc tcacttggat tcaaacccgt ctctttaata atatgttcta
     9841 ttaaagtcgc acctaaatca taggctggca cgtctttaaa cgcacctcca aaaacgccaa
     9901 taggtgtcct gtatgctgct gctaatacga ctctcgtcat gtcatattca tctccttatg
     9961 atatatacaa ttttattact aatacgtcta atgaaccaat acgccactaa catccaattt
    10021 atgaaaccaa ttgctacgta tgttacattt tcattcttgt tatgcattta acaaatcata
    10081 ttatacaatg atgtaatttg tttgggcaat cggaaacgta aacggtttca ctttaacatt
    10141 tcaccctctt aaatatgctc tatcatcctc atctatagta caaaattatg ctttgattta
    10201 ccacaaatta tcattaatca tttcgcgtaa tgactctaat taaattctat ctatcattgc
    10261 atcgctattc acagctggtt tcaccatcaa tttctcatca attttttatt accatgacgt
    10321 ctccagtcaa atattaaatc gctgatgatt gttaattccg caattacctt gcagtcacct
    10381 ctccttccac ttcatcgcca aatctgcccc tattcactag aaaaaataaa tagtatactt
    10441 agtaaattaa ttacacatct taaaggagtt aaaattttat ggttaatgca ttaacaccaa
    10501 aacatttagc tgaaaagcgt gcaggattcc atgagttgtt ctttgattta attttcgtct
    10561 atgccattca aaaaattgca catgtgattt taacaacaca aaacggttcc atttcagctg
    10621 atttattttt caaatacatc gtcatgagtt tgttcttatg gttaatgtgg tctcatcaaa
    10681 cattttttac aaatcgattt ggtcaagtta cattcaaaga cgtctcgttt atgatgttta
    10741 atatgttcat catggtcttc ctatctaaca gcctatatcc ggactttgaa aagacattct
    10801 tcccattctt cttatgcgtg gcaattatgt atttaagtat cggattacag tatttattac
    10861 atatacgtac agggttagat tatggcgata agcgtacatg tcaagctttt gcgagcgtag
    10921 cctttgtgat ttcattttta tcatttctat cattagtact accacaaagt atccattaca
    10981 tccctggatt tttaggcgta ttcattgcag cgacaggact tattccattt cagaaatatc
    11041 ttgtattatc acctgttaat atgatgcatt tagtagaacg tttttcgtta ctaacaatta
    11101 ttatctttgg cgaggtactg gttggtttag cttcgtcatc atttagcatt gatcatttta
    11161 gttacattta tatattccaa tttatgattt taattagtct tttcggtgta tattggatca
    11221 ttactgaaaa ttatattaat cataaattaa catctatcgg tttccgttta tcgtatacgc
    11281 atctattaat caatatcgct ttaggtgtga ttaatgctgc aattgttttt agtaataaca
    11341 ataaactaaa tgacttgttt gaaattaata tgatgtatat cagtgtttta atattctata
    11401 ttggattatg gttaatcacg ccttatttcc ataatgaact gacaaacgcg aagtatattt
    11461 catattcact aggtatactc gttatgtctt atattgtcag ccttttattt aaaggtcacg
    11521 accatgtaat gattatctca gtatcagttg caaccttctg catcatgctg atttatttta
    11581 aaaatcaacg tctacgtaat aaacatgaat aaatagtatt tacctcggtc aaagtattgg
    11641 aactgaatcc aaaagtgtcg aacttcacat tatacttttg tgacaaactt cagttatgct
    11701 acatgatcga ggtttttaaa tatttccgag gaaatcgtta gcctatacaa tcatcaaatg
    11761 atacttataa ataacatata tcctctaatc cccactatct aaaaggaaat aattcccaca
    11821 caacataacc acctcaaaac aaataaatca atatagatat gtcacctata cttatccccc
    11881 acatttcatt ccacaccaaa aagaacagat gacaatcgta aagatttatc acctgctcta
    11941 aatattattt caaatactat catcaccaaa atcgacaatt atagttcttg tttgttaaaa
    12001 attttaatag acaatatata agaaacgctc gttataacta taccaatgac cgcatatgct
    12061 atgacaacta gccatccttc agtgctaatt ttttcaaatt ctaatgcaga tgcaccacta
    12121 accataccat atataaagaa aaagaccacg aaagaaataa gtagtagtat agatgcacat
    12181 atcataatga cattggagtt ttcagcgcca aatttaaatg tcaatgggaa aatgatagag
    12241 tatgccccta tgataccaaa actcattaaa cctgacataa taccaatcat cacactttgg
    12301 gtcacaatcg tagtcacaac taatccaatc attaaacttg caccgaataa gattaaataa
    12361 aaggcaaagt atgacttaat ataatcacta cgtttaaccg gtaaagtaga tacataatac
    12421 atccatcttg agtctttttc atgtttaata ttatcagtaa taggtgtgat taacataacc
    12481 ccagccattg ccgaactcat caacggatta aatactgcaa agtatcctgc agctataata
    12541 gcgactataa aataaatata tgtttgcttt ctcgttgcat aaaaactact taggaacata
    12601 cctttcatta tacttcacct cgcattatga tttttgtagc gtcatcaatg tgttttaaag
    12661 gctctgcatt ttcaatttct gcataatctt taactaaaat ttgatatttc ccctttgaca
    12721 atctagaagc aatgattaaa tgcttaggaa tttcaacatc tttatcttct gtcgtaacaa
    12781 ttccatattg tgctaaaagt atatctttct gttcagttaa aatcattcgt ccatctttca
    12841 taaacactaa tttatcagct aaatgttcta tatcttcaga aatatgcgat gatattaaga
    12901 tgcctccacc ttgtgcgaca aaatcttcta gtatttccat tacttcttct cttccagaaa
    12961 catccatacc tgcagttgct tcatctaaga ttaataactt cacatcatga gaaagcgcaa
    13021 tcgttaaagc tatcttcatt cgcatccctc ttgaaaaagt tttaatttta gtttgtagtg
    13081 gtaactcgaa atatttgatt aaatcaaaga atttttgact attccaagtc atataaatag
    13141 attgaaatac tttatcaata tctttaatag tcaatttatt aggcactctc aaatcatcaa
    13201 acactacacc tatgtgttct ttatattcga catcatccac ggtatgttct tctccaaaaa
    13261 atgtgatact accgttatct ttatgtcgat tgcctactag aatattaatt aatgtcgatt
    13321 tacctgagcc atttttccca attaatccaa ctgtctcatt agtagaaatc gttaaagatg
    13381 catcttgcaa tttaaaatcc gaatctttgt attgtttgtt cacactatct acgtttaaca
    13441 atgttgtcat atccatgctc ctctttgttt aattttaata aaaacgtctt ctcttcaata
    13501 agtaactaaa ggcgttatat atgaaaatag caatgataag tagcaaacta aagctttgat
    13561 taattccagt agtaattgaa taaagcccaa ttactacaat ggctagtatc aatattgata
    13621 agttgatcgc atatgtttta aataatgcaa ttaattctat atggcgttca ccttcatcca
    13681 atatttccaa tcgcttttca gtgtaattct tatctgcaat ttttggtatt ctgtcatcaa
    13741 accttctatt aaacagtgta aattgtgtat tgaaaatagc actagcaaaa aatggtatta
    13801 aaaagaatag tattgcattt gctgcagcgt gccctaccac aaaaataagc aatactaaaa
    13861 aagcaataac tgtctgcaga atacttaata tacttccatt taaaacataa cgattcgcaa
    13921 gcaactcata ttgatctgtt gcatcgatat ctacctcttt atctacaagt tgcttatact
    13981 tcaatgcacg tctttgattc atcaaaacaa tggcttctac caatatgata ataatcgtcg
    14041 caacaatcga tattacaacg acattgttat atgtcgcaaa cgtcaagttc tcaataccaa
    14101 atccatcaaa attacctaaa attccaccta tgataccccc tacaagtcca cccagtaaaa
    14161 gatatccgat atatcttagt attttcatgc ctcatcctcc tcaataataa aaacagtttc
    14221 caccgtttca ttgaaaatgc gagcaatttt tattgccgtt aatactgatg gcataaaatt
    14281 gtttcgctca attagcgata tggtttgtct tgaaacgccc gcttgtttag caagttgcgt
    14341 ttggtttaag ccatctcgtg ctcgtaattc tttcaatcga ttacgcacat cgcatcaact
    14401 ccttaattac actgtaaatg atatgcgtct ttttgacaac tatatttgtc aaatctacac
    14461 caaaaaatat gattatccac ctatgtatga cattttgaaa caaacacctc aacgcctaca
    14521 agtcataatt gtttactttc gttacacctt cctgcataat taacagcatt ctaattttag
    14581 tatgatgcac gcattttcac taaatcaaac cattcaaagg agactattat ggcatttaca
    14641 ttatctgcaa ttcaacaagc acatcaacaa tttactggtg ttgactttcc aaaactattc
    14701 aaagctttta aagatatggg gatgacttac aatatcgtca acattcaaga tggcactgca
    14761 acatacgtac atcaatcaga agatgatatc gttacgtcat ctgtaaaaag taatcatcct
    14821 gttgctcaaa aatcaaacaa aacaatagtt caagacgtct taactagaca tcaacaaggg
    14881 caaacagatt ttgaaacatt ttgtgatgaa atggctgaag ctggcattta taaatggcat
    14941 atcgatattc aagcgggcac ttgtacttat atcgacttgc aagaccaagc tgttatttca
    15001 gaattaatcc ctcaataaac tatatttata gcaacatttt aattatttca taaaatttta
    15061 ttgataatca ttatcgttcg gtataaagta aatactatat actacttatg agtgaggttg
    15121 attatcatga taactaacac ttttatttta ggcatcacag gcccaacaag tcttgtcgtc
    15181 attagcatta tcgctttaat tatttttggt ccgaaaaaat taccacaatt tggccgtgcc
    15241 atcggttcta ctttaaaaga atttaaatct gcaacagaag atttagataa agagtctcac
    15301 gatacaccca gtaaggaatc gaaacaacag cgagagcaat agcactgacc acaccttact
    15361 ggttcacttt agcgaactac gccatcggtt agtaaaaatt ttattgtcgt tcgtcattac
    15421 ggtcatcgtc gtatatgttt catcattttg gtggatgaca ccattcataa cgtatattac
    15481 ccgtgcacat gtgtccttac atgcattttc attcacagaa atgatacaaa tatacgtgat
    15541 gattatattt ttcatcgcgt tttgtttcat ttcaccagtc atgttttatc aattgtgggc
    15601 attcattgca cctggattac ataataatga acgtcaattt atttataaat atagtttttt
    15661 cagcgtgtta ttattctgtg ctggcgtcgc ttttgcattt tatgttggct ttccaatgat
    15721 cattcaattc gcgttaaaat tatcattaac tttaaacatt tcgcctgtca ttggctttaa
    15781 agcatatttg attgaactca tacgttggtt attcacattt ggtcttctat ttcaattacc
    15841 aatattattt atgggtcttg caaaattcgg tcttatagat accacatcat tgaaacatta
    15901 tcgtaaatat atttatttcg cttgctttgt attagcaagt atcattgcac cacccgattt
    15961 aacattaaat atattactca cattgccact catattgctc tttgagttca gtatgttcat
    16021 cgttaagttc acatgccgag gcaaaccgcc aacacattaa ccttatacga atcaatgctg
    16081 tgattatcgt tatactgcaa taccgaatat ttccaatata gcaacaaaaa aacaggcact
    16141 cggcctgttt tttatttttt aaatttaaag attaagatag caactaccat aatataagca
    16201 ccttgtgcta tcaatggttc ataacttgga taaaagccca accagttaat cgttgggaat
    16261 ccttcaataa catgtcttgg catcgcacct aataattgta acttttgaat acttacgcca
    16321 agcattttga atcccataat aaaaataaag atcgacaaca ctctaaatat atagaaaata
    16381 ggtattaatt taactataaa tctaaataat aatgcaaaga tgattaaaat aacgatagct
    16441 aaagcaatac caataataaa atctttggtc gctagctcac ctatcatccc catatagaaa
    16501 atgataacct cgacaccttc acgcaacaca gatattaaac caatcgtcgc taacaatacc
    16561 aaattaccat tactaatcgc attagcatac atatttttaa tcatgtcatt ccaacgtttt
    16621 gcatttgaac gtttgtgcat ccaaacacca acgataaaca ttaatatgac cgcaacgata
    16681 cctaatcccg cttccatact ttcacgaaga atgccactat tccctaaagt ttctacaaac
    16741 gtaattgcta agataatact cagtacaagt ccggcaattg caccaccaat cacacttgca
    16801 gtccctttct tatcttttac attacgcgtc atggtagtca atgtcattac aattaacaac
    16861 acttctagcc cttcacgtaa aaagataatc atcacatcga cgaagctata actatggcca
    16921 acaacctctt taatttggtt atttaaatct actaaaccat ctttcacatg tgctttatta
    16981 tgttcgtcta atacactttg ataatatggt attttatctt caattttcgt atacaaagca
    17041 ccgtctttag tttgaatttg accttcaaca tacggccaag tttctataaa atgtgtaagc
    17101 gcagcatcag catccgacaa ttgattgtcg tcgatagctt taatcgcctt ctctaacgca
    17161 tcatttaatt gtgatacatg gtattgatca tttgcagacg tattactttt tttatcgaca
    17221 tgatcaatat ttgatttaaa agttgtccaa gcatgtgaca cttttgccgt atctaatggt
    17281 gacttatgaa ttgcaattct aagttgtaat aatgcgactt caatttgtcc atattgattt
    17341 gcgtcataat tgcgaatcac tgtttcatta cttgtccaaa tctgattcaa actattgttc
    17401 aaagattcta attccgcttt atttttatct ttaatcgctt ttgtcatcgc agcatcttta
    17461 gcatcgactt gctgttgcaa tagtttaatt ttagaacccg catctttact agccaatttc
    17521 tcttcataag caattaatga cttcgttaat tgcgaaagtg tatctttttg attatcattc
    17581 gcttttgcat cttcaagctt tctcacatct gatttgacag cattactttc actattatct
    17641 tcaagcgata atttcttaac tgcacttacc acttgctcaa ttgctttctg cttattgtca
    17701 ttcgatatcg aattattaga aagtgcagat ttcgcatccg ttatcacact atatacatca
    17761 ctaatacttt gttgttctgc tgcctgactt ttcagtaacc caaagctaca caccatagca
    17821 gcagttatta gcattgctac aaatttagtc aaataatgtt tcaccaaggt atcctccctt
    17881 actaacacct ggtaatacta aaaatgaagc agaacctcta tgtgtaatat attcatttaa
    17941 tttatcatta ctacctaaat tattttgtat atcgataaat tgttttgtcg ctttttgaaa
    18001 agcaataaaa agtaagcctg tttcgaagtt acctgtgcgg tcatccgtac catccacata
    18061 attaaaggct ctacgtaaaa ttgacgtatt tgcttctttc gctagcctcg tatgggcatc
    18121 tttatcaata atatactcgc catgactatc tttcgctttt aagtcaattt catcaaactc
    18181 tttcccacct gttaacggtg caccactatg tcgtttccga ccaaatgtag cctcttgttc
    18241 ttccagcgca gtacgatccc acgtttcaat gtgtatttga atacgtctga caacgcaata
    18301 agttccatgt ttcgcccatc cgtcatcaat aaacacataa tctttaagtt gattattctt
    18361 cctaggatta attgttccat ctttaaatgc cattaaattt ctaggtgttt ccttaccttt
    18421 agcagagata aaaccagatt gcgcccaacg taccttaaca atatctcgaa atggacgaac
    18481 taaattatga accgcatgaa aggatacttg cgaatcattt gagcatgctt gaatcataat
    18541 atcaccatcg ctgtaatcgt ctattaactg atcattcgga aaatgcggta aatctttaaa
    18601 ggcatcggga attttactag ataacccaat tttcttcatc aaagacttac taatcccaaa
    18661 ggtaatcgtt aacttgcttg cacctaatcc tatcgattca ccggtatcta ctggtggcat
    18721 taaaggattg ttactcggct tgccaactgt atcaccatcc atcatacgat cagccataac
    18781 cgtccacttt ttaaacattg ccttaattgc atctctatct tttgacttca aatctaacgc
    18841 aacgaaattg catgtttttt gcgtgggtgt ggtaatgcct ggttgcactt taccataaaa
    18901 ttcatacgca tctttttccg gatcttctgg tgtattgaac attgatttga aagaccacat
    18961 gctaccaaca ccacttgcgc caattgcaac accggcaccg ccaataccta acattttcaa
    19021 aaatgtacgt cttgaaaatt gcgtactatc gttaacttgt tcataatttg tcattcagta
    19081 atcacagcca ttttacttag tggctctcct aatgcattaa cagcatccgc aaatgcttta
    19141 cggtctttct tcgatacttt ttcaaatgac tcataaccgc cgttgttatc tttatattta
    19201 tccaataatt gattcacttt atcgaagttc atttggatat catcacttaa ttttttatct
    19261 tttttctcta aaataggttt aaataagtca taaatttttt gtgcgccttc aacgttcgct
    19321 ttaaaatcat ataaatctgt atgtgaataa atttcttctt cacctgtgat tttagaagtt
    19381 gcaacttcat ttaataggtc aacagaacct tgtaacatta attttggtat gatatctaat
    19441 gtatcagctt tggcatgcaa ttcttttgca tctttcaata attgttgtgc atctttttta
    19501 gtcacatcat caattttctt atcttcgtat aatgcttttt caatcttatg atatcctgac
    19561 cattcttttt cctttttctc ttctttcata tctgcaagac gtgcatcaat tttaggatct
    19621 aaatctccaa atgcttctgc aactggttca gagcgttcat aatacatacg aacttttgga
    19681 tataacgctt ttgcttgggc catatcatta ttttcaatcg ctttaacaaa tttttctgta
    19741 ccttttaaaa attcatttaa ctgttcatca gtatatttat catatgcttt tgttgcttgt
    19801 tttaattctg ctttaacacc atcatctttt ttcgatgtct ttgaatcatc cttcttacta
    19861 tcgtcgttcc cacatgcagc aattaataac gttgatgcta ataatagcgt tgttaacttt
    19921 ttcattttga cacctcatta tagaaattct attatcccca tagatgtgtt cttataaata
    19981 ttattttcgc attccctaca cgctaaaata atacattaca cttaaaacgg ctgttttaaa
    20041 gcatcctccc ataaacatca tctagttgat aataattatc aaaatcatta tattgttata
    20101 aaataatact gtcaacaacc ttttattttt aaaaattaaa taaaagagcg ctgccatttt
    20161 aaatgtcatc actcttttgt aagtctaatt tgtcattttt ggtcgtattc tgattttaat
    20221 aaactataga tgtaactcga tgaataaata ccatttagta attcattgtc acgtaacata
    20281 ccttctctag taaatcccag cctttcagga atagcccggc ttttttcatt attaactgcg
    20341 gcactaatct caattcggtt taagtcaagc tcttcgaaac aatactttat caatgcctca
    20401 actgcttgtg tcataatccc atgaccctca aattctttat ctaaatagta ccctaatgaa
    20461 gtttttctgt gcatgtgatt aatttcatgt aaaccgatga caccaactag cgttccttca
    20521 taccaaatgc cacactgaaa tccattacca tcagcaaatt gcaaaagtcc tcttttaata
    20581 aatgcacgcg tatctgatgg ttgctcagtt gcatctaccc aaggtaacca ttccctaagt
    20641 gaatttcttg aacgattgac taaattgaaa agcgcttctg tgtcatgagc ttctaaaatt
    20701 tttaatgtta tttgttcatt cactttcatt ccaaacatac catcacatcc tcattcattt
    20761 ttcatataat tctgtaaatt atcattataa taacatatta atgttacgca ttgtatctta
    20821 aatttgttgt ccttccccaa cttgcattgc ttgtagaatt tcttttcgaa attctctatg
    20881 ttggggcccc gccaacttgc attgcttgta gaatttcttt tcgaaattct ctatgttggg
    20941 gccccgccaa cttgcattgt ctgtagaaat tacggaccca atttctctat gttggggccc
    21001 atccccaact tgcacattat tgtaagctga cttttcgtca gcttctttgt tggggccccg
    21061 ccaataagcg ttgtagtgcc tattactttg attgatgtcc aagtttccta tcacacaact
    21121 taatttaatc actactttat tattcattcc aaatttatat ttaaaaatac gacaaaacag
    21181 tgctaaaatg ctattaaagg aggcactgct catggcatca ttgaaaaata agcattttat
    21241 gattgggcta tcactaacat ttatagtcgc attatttagc tttttagcag ctaaattgcc
    21301 aatcctagat aaggttggcg cattaactat cgcgatatta atcgctattc tatatcgtca
    21361 ctttagagga tatcctgaac aatatagctc aggtattacg ttttcatcta aatatttatt
    21421 aagatttgca atcatcctat atggacttaa actcaatata ttcgatatta tcggtcaagg
    21481 tagtaaacta ttagccatcg atgtaggtgt cgttattttc agtattgtaa tgatgctctt
    21541 tgtcaacaaa ctgttgcatg gtgacaaaaa catcgcatta ttactaggtg tcggcacagg
    21601 cgtttgtggt gctgctgcta ttgccgcagt cgctccaata ttcaaatcac gtgaaaagga
    21661 tacagctatt agtatcggta tcatcgcatt gattggtacg atattttcac ttatatatac
    21721 agctatctac gctatctttt caatgacgac aaatgtttat ggcgcttggt ctggtgttag
    21781 tcttcatgaa attgcacacg ttgtcttagc tggtggcttt ggtggtagtg atgcacttaa
    21841 aattgcactt cttggtaaac ttggtagagt attcttactg attccattaa ccatcgtact
    21901 tattttaatt atgcgtttcc gttcatcaga atcatctagc aagggtcgaa taagcattcc
    21961 atacttttta attggatttg tcattatggc cttagtgaat acatatgtaa ctattccgtc
    22021 agcattgcta aatattttaa atactgtatc aacaatttgc ttattaatgg ccatggttgc
    22081 tttagggtta aatgttgcat ttaaagatct taaaaatcgc gcacttaaac cgcttatgac
    22141 aatcattatt acctcaatat gcttgtcatc acttgccttt atcgtcgttc attggctata
    22201 tagttaatat gaaaagacgc tcaaacactt acaaatttag tgtgccgagc gtcttatttt
    22261 tatactttaa aagtcgtatt ggttgttttc ttcttcaaac acttgttgtt gtactttggc
    22321 agttcgcatt gtggattgtg ccaacgcttt taaccgaaat acaacatcat cattgacaat
    22381 ttgttgattt gaaaaatctt tctcttcaat aaatacatac gtttgcatcg tatgtgcttt
    22441 catgtaactc aatattggtt ttaaatgcat ttcaggaatt aaataatgtt tactagaacc
    22501 tgctgtcgct acaagtccta ttaccttgtc acgaaacgca ttgactggaa gtagatcaaa
    22561 cacatttttc aaagcaccag ggatggaagc ttgaaaaatt ggaaaaccaa taaaaatcac
    22621 atcagcctgc attaacgacg tcgttaattt atatacatct cctgtagtat ctagataatt
    22681 ctttccaacg ctaaattcta attcaagttc tcgtaaatcc atcaactcta tttgatgtcc
    22741 ctcattgatg acttctagtt cattttttaa atcatccata gcaattctcg ttttagaacc
    22801 tactgtggaa cctgacaata atacaatatt catgatgacg cccctcctat tttgataaat
    22861 gctttttaat cgccggtata atgtcgttgc caattaactc aatattcttc ataacatttt
    22921 caaatggcac accgccaaaa tcaagctgtg ccataaaacg ttgatgacca tacaactcgt
    22981 gttgatacaa tatcttttca ataatttgtt gcgggcttcc aaccattagc gcttctcgat
    23041 aatctgacga attagcaaat tgctgtttcg gataaccaac accacgaata aatgacatcc
    23101 ctgtattcaa atgtggataa aattctctca tagcatcctg agttgtttca gctgtataaa
    23161 acagactcgc tgtacttact ggtaaagact taggcgaagc atcgaaacct gcttcagttg
    23221 ccgcttgacg ataagcatct atagaacctt taaagttcat tgctgggcca ccaagggttg
    23281 taatcatcat tggcacacct tgttttcccg ctttaatagc acttgcaggt ggaccaccaa
    23341 cagcacgcca tattggcaat atattatcga ttggtcttgg gaatattttc atatttctaa
    23401 gttctggacg atattttcca gaccaagtaa tacgctcagt tttatttaac tctaaaagta
    23461 aacctaattt ttcttcaaac aattcatcat agtcttttaa atcatagcca aacaagtcaa
    23521 aaatacctgt tcttgatgct ctgccagcta caatttcggc tctaccatga gaaatcaaat
    23581 ctaatgtcgc gaagtcttca aatactctta caggatctgt tgcactaata atcgttgaag
    23641 aacttgaaac tttaatatgc tttgtcgctt gggcaattgc acctaacaca accgtatgtg
    23701 cctgtgttgt aaaatgctcc tgatgacttt caccaactgc aaaaacatca atacctgctt
    23761 catctgctaa tttacttgct tcaataattt cattaatacg ttgctcataa ctaacttttt
    23821 cacctttcaa tggattcaat aaatgatcac ctaaggaata caaaccaaac tcaagaggcg
    23881 tatttttatt catttctaat ttggccatta aacattctcc tttatatttc gtgtaactta
    23941 ctttcaattt gttcacgttc tgcttctaaa aagtcaggca agttcaattg acttcctaat
    24001 tgttcaacag gtgtatcaat agtaaatcca ggcgcttcag tcgcaaattc atacataatt
    24061 gaattatggc gatagtataa tgatttaaag aaatagcgat caattatacc tgaattactt
    24121 tgtggttgtt gttgtaattt cttgtaaatt gcatctaagt cactcatttg tggtgtatta
    24181 actgcaatat gatggatata acctcgtcca ggtctttcac gttgtccctg ttgctcaata
    24241 actacaaaat ccgaatataa accttgtggt gctaatgtca gcacatcgaa tgatttatta
    24301 tctcttttgc gataacctaa gacattttcc aaaaattcta ccgtacgcgc tgcatttcta
    24361 actcttaatt cgactggccc tattcctaaa atttgatatg cttgaggtat ttcactataa
    24421 gcgttatgtt gccattgatg tggtacttca aaactatcat taacaagcaa tatgatttct
    24481 aagtcgtctt catctttaaa gacaacacct tcttgtccaa gatagtacaa acgttcagac
    24541 ttaataccat tattctcaag acgtttttca aaataatgaa gtgccgcttg atttggtact
    24601 aataatgaaa atcgataaat cgtttcagta cctggctgct tatgacccgc attgggaatt
    24661 tcaaaaaagc ttaaaattgt tccggctgta cctacttcgt ccccataaaa caaatgatac
    24721 attgaaggat tgtcttgatt aaccgacttt tcaactaatc gtaatccaag gacatttgtg
    24781 taaaaatcct tattacgttt tgcatctttt gtatacatgg atatgtgatg atgccctact
    24841 atattcatac tatccctcct attagtataa attttatacc agcttacata aaaaagatag
    24901 ttatatgcca tttacccctt gcaaataaaa atatgtttgt actcattcgt agatacacag
    24961 ctagtatgta taacgacata ataattgcat aattataaat ttatgtgtag caatttattt
    25021 tttctaaatg tttataaaat acttcaataa ctaaatatgc aagatttcac aaaagcatat
    25081 tgacaacgct ttcatacatg tctaatataa gtttatcgca caaaatgcat ataaatacat
    25141 attaaggagg attttatgaa ttttcttaaa cctgcaaagc atattaagcc tttgccagaa
    25201 aatcagatag atgataccta taaacgatta cgtctccaag tatttcttgg tattttcatc
    25261 ggttatgctg ggtactattt attacgtaaa aacttttcgt tagcgatgcc ggcattgcaa
    25321 gagcaaggtt ttacaaaagc ggaactaggt tttgcacttt ctgctgtttc catcgcatat
    25381 ggatttagta agttctttat gggtactgta agtgatcgga gcaatgctcg gatattctta
    25441 gttcttggat tagcactcac tgctatcgtc aatttgttaa tgggatttgt accgttcttc
    25501 acatcaggta tcggtattat gtttgtccta ttattcttaa atggatggtt tcaaggtatg
    25561 ggctggccac cttcaggccg tgttctcgtt cactggttta gtgtaagtga acgcggaagt
    25621 aagactgccc tttggaacgt tgcgcataat gttggtggag gtattatggc acctattgct
    25681 gcttggggta ttacaacaac tgcatttatc aactttggtt atttaaaagg tttcgaaggt
    25741 gtattcattt accctgcact cttagcactt atcattgccg caatttcata cgtattgatt
    25801 agagacacac ctcaatctca aggtttacct ccaatcgaaa tttataaaaa tgactttgct
    25861 acaagcgata agaaaacatt agaaacagaa ttaactacaa aagaaatttt atttaaatat
    25921 gtactgaaca ataaatgggt atgggcaatt gcctttgcaa atatatttgt ttatttcgtg
    25981 cgttatggtg tacttgattg ggcgccagtc tacttaagtg aagaaaaaca tttcgactta
    26041 aaagcatcag gttgggcata cttcttatac gaatgggctg gaattcctgg tacattatta
    26101 tgtggttaca tttctgataa attattcaaa ggtcgtcgtg gacctgcagg tttcttcttt
    26161 atgttaggtg tcacagtatt tgtattaatt tattggttaa atcctccagg caatgcttgg
    26221 ttagacaatg tctcattaat tgccattggt ttcttaatat atggaccagt tatgttaatt
    26281 ggtttacaag cattagatta tgtacctaaa aaagcagctg gcacagcagc tggattaaca
    26341 ggattatttg gttatctgtt tggtgctgta atggccaaca tcgtcttagg tgctgtagtt
    26401 gataaattcg gatgggatgt cggttttatt ttattaacag caattagtgt gtttgcaatg
    26461 ttgagcttta tcctcacttg gaataaagta ggacaagaaa ccgttcatca ttaaatgata
    26521 aaaaataaag tcatatggtt atcttatcga aagatgatat attcatctct tataagttca
    26581 accatatgac tttttattag tattcaaaaa aatatttaca ttgccacttt tgtgtttgcc
    26641 ctgctgtttt attcaattga ttacaccact taggataaac tctaaaagcc attttccctt
    26701 gatacttaga tgaagctaag ataccttttt ctaccaataa ttccctagga aatagaaagt
    26761 agccatttaa ttcatcatcg ataacagcaa caatcaggta atcagcaaac tcttcaattt
    26821 tgtatggccg attataatta tcttcgtctt ttgtccaaca tgtcacgaaa tatcccgatt
    26881 tagtcggtgt tttcttagct aacctacttt gatatgtttc ctctttaaag ctaaatgtta
    26941 atgcttcgta atcttgatta tatttttctt cagttaaatc tttaacttct gattcttcac
    27001 taaaaatatt tttcaacagt attttagatt tacacatatg ccaatctcac tttatttttt
    27061 cataatcgta tcatatattt atttttttcg aaaaatacac ttccgaccga aatatactca
    27121 gccagaagtg gacgaaatat ttaaccttct attaattcag atgtatcaac tgtcaaacga
    27181 tcacgtaata aatagacgat taacattgct gctaaagcac aaagtgactc agcaattaat
    27241 aatgaccaaa tgacacctgt tagtccaaac aaagcattca taataaataa tactggaatg
    27301 ataattgcac cttgtaaaat ggccataatt gtagcaccac gaccttgccc agtcgcttga
    27361 agcataccag taaacaagaa acctatacca tttaataata atgatgccat tgttactttc
    27421 aaaataaatg tcgccatctc aacaatggct tgatcagtag taaatagtcc gaccatatga
    27481 tgtccaattg taaatacagc actcatacat acaacaaaga taacgccgat agacatgata
    27541 actgctttga taacgtcttt catacggcct ttatttgcca taaagttata tgcaattagt
    27601 ggtacaacac cttcacataa tcccatgata ataagttctg gaaattgcac aagtctaaat
    27661 gagataccat aacttgcaat cgcgaagttt ccataatgtg ctaaaaataa atttaaaact
    27721 aatcctgtga atcccattaa gatactcatt aaaaatgcag gaataccgat tttaaagatt
    27781 tcagaaagca tttctttatt aggtttcgca agtttaatat taactgacac aacgtcacta
    27841 tttttcataa aataaatgat aaagaacaga gcagcagcaa cattactgat tgcagtaccc
    27901 aaagctgcac caacaacgtt taaatcaaaa ccaaaaatta aaattggatc taaaataata
    27961 tttaagccta cactagctaa cataccaatc atagaaacca ttggtgcccc aattgcacgt
    28021 gcaaattgtt ctaatatgaa gaacaaaatt acaaaaggtg cacttaaaaa cattactttc
    28081 aaataattac ttgttaaagc taacgtttca cctctcgccc ctaaaattgc tgcgatttga
    28141 tcactgaatg gtaaagtaac taaaatcacg ataagtccta gtgcaatacc accataaata
    28201 gagaaactac ttacaaattt actcttacta tagtctttcg cacctaataa acgtgaaata
    28261 taagttcctg caccaacgcc aaataaatta cctaacccca ttaagatagc aaatactggc
    28321 agtgttagag agatagcaga aatcatgtgg ctatcttcta aaaatcctat aaagtaaata
    28381 tttaatatgc cataaataac gcttaataaa gtccctatca tcattggcaa tgagaaatgc
    28441 atcatcgctt taaatactgg cgatttctca aaataatata attgttcgtc tttcattgtt
    28501 caatactcct tgtcttttcc aataattagc ttactaacaa ttagatatct aactataata
    28561 ttaaagacaa agtgactgat ttctaccagt cacacttatc atttattgta aactagataa
    28621 cattttagtt aagtttgctt tcatttgttc attttcttct tcagataact gcgatacgag
    28681 tgtttgttcc atttcatcaa atatcgaagt gaatgcttct acgagtttaa tcccagaggt
    28741 agtcagccct atattctttc ttctcgtatc ttgtgcatcg acatagcgat agatcagctt
    28801 tttacgttca aggttcctta ataaattact gacagttgga cctgttcgtt gtaatgcttt
    28861 agcaatatca ttttgtgtca gtccatcttg ttgatgtgca taaagataac ctaacgtatg
    28921 accttgctca tttgtaatat caaattgctc taacttttga tcagcctttt gtttcatctc
    28981 atgactaatc attctaaata aatacgaata agtgaattcc atttattgca ccaccatttc
    29041 gttagatgtc taactaaata tactgcgaca tttttaaatt gtcaacatga atatctaatt
    29101 tattttaaaa gttcctataa aatcttgcgc cataaacatt ttgatatgca atttttcgga
    29161 ttaaaatatg aaagagattg catatctacg tttttaagaa atcgcttaca attacaaata
    29221 ggaggatatt acgttgttaa gttatagaca aatcgaaatt ttgtatcttt taattcatga
    29281 gcaaacgttt attccaatca ataccattgc taatcaactt ggtgtatcac ctcgtaccat
    29341 tcaatatgat atagcatata ttgagcaata tgctgaaact tatcattacc aagtcagccg
    29401 taataaagct gcaggcatca aagtaacaac agctcacgcg acactattaa atgaattaga
    29461 gcacaatcta acaaatcaaa ttcatttttc aaaagatgaa cgactcacac atatcgcttt
    29521 aaagttattc gaaacaaccg atcctgtttc aacaaagcaa cttgcgcaag atgttaatgt
    29581 ttcgcgtcgg acaattgcag atgatattaa aatgattcaa gcccaactag atcaatacca
    29641 tttaaagtta aattatgtcc acaataaagg attcaatatt attggtgagg aagatcatta
    29701 tcgtaaagcg tatgcacact ttatacatca atatatgaaa caagctgcac cttttataga
    29761 ggcggatatc tttaattcag aatcaatcgc attggttcgc cgtgccatta ttaagacatt
    29821 aaatagtgaa aattatcatt tagttcagtc ggctatcgat ggcttaatct atcatatact
    29881 cattgccatt cagcgtttaa atgaaaattt ttcgttcgat atacctatca atgaaattga
    29941 taaatggcga catactaatc agtatgctat tgcttcaaaa atgatagaaa acttagaacg
    30001 cagttgtaat gtcacatttc cagagtctga aattatcttt atcacgttac atttactagg
    30061 ctctaaaatg actgaacata cagcatcttc aattaccttt gaataccatg atttatcgca
    30121 aaatatacat gaattgatca cttgtgttag ccaagaatta ggcattgata tgtcaaaaga
    30181 caacaagtta cataccagtc tgatcacaca tatcaaacca gctatacatc gtattaaata
    30241 cgatatgcta caacctaatc ctttgaggca agaagttatg cgtcgctatc ctcaaatcat
    30301 tgaagccgtt agcaagcata ttagtccaat tgaacaagat gctgctattc gcttcaacga
    30361 agatgaatta acatacatta caattcactt cgcatcaagt atagagcgtg ttgcaacaca
    30421 taaacaatca atgattaagg ttgtcttact atgtggttct ggtataggca cgtcacaact
    30481 tttaaaatca aaactaaatc acctgtatcc tgagtttcac atttgggatg cctattccat
    30541 ttatcaattg gaagaaagtc gattattgca agataacatt gattatgtca tttcaacagt
    30601 accttgtgaa atatcagctg taccagttat tcatgtcgat ccatttatca atcaacaatc
    30661 tcgtcaaaaa ttgaatcaaa ttatcaatga ctcaagagaa caacgagtca tgaaaatggc
    30721 aactgatggc aagtcactcg cagatttatt gcctgaacat cgcatcatta taaataaaca
    30781 accattatca attgaatccg caattgcagt ggctgtgcaa cctttaatca atgatggcat
    30841 tgtctattca aattatacag ctgcaatttt aaaacaattt gaacaattcg ggtcatatat
    30901 ggtcattagt ccacatattg cacttattca cgctggtact gattatgtac agaatggtgt
    30961 aggtttcgca ctaacatatt tcactgaagg gattatcttt ggtagtaaag ctaacgatcc
    31021 cgttcacctt gtaattacat tagcaacgga ccaccccaat gcacatttaa aggcattggg
    31081 acagttaagc gaatgcttaa gcaacgactt atatcgacaa gatttcttag atgggaatat
    31141 ttttaaaatt aaacaacaca ttgctttaac tatgacaaag gaggcttaat aacgtgtcat
    31201 tagacatttt gtcaacaaca cgcatcattg taaaagaaca agtaaatgat tggactgaag
    31261 ctataactat agcttctcag ccattactac aagaacaaat tattgaacaa ggctatgttc
    31321 aagcaatgat tgatagcgtt aatgaacttg gaccttatat cgttatcgca cctgaaattg
    31381 caattgcaca tgcaagaccg aacaatgacg tacatcaagt tggtttaagt ctattaaagt
    31441 tgaatcaaca tgtggcattt tgtgatgaag atcactacgc atctctcatt tttgtattga
    31501 gtgccatcga caatcattca cacttatctg tattacaaaa tttagcaacc gtactgggcg
    31561 ataaccaaac agtccagcaa ctattaactg caacaaatgc acaagacatt aaaaacattt
    31621 taaaggagca tgattaatat gaaaatttta gtagtatgtg gccacggttt aggaagtagt
    31681 tttatggtag aaatgaacgc acaagaagca cttaggcaac ttaatgcacc atctgatatc
    31741 gaagttgaac atagtgacat tatgacagca agtccagaga tggctgactt gtttatttgt
    31801 ggtagagatt tagctgaaaa tgccgaacgt ctaggggatg tcttagttct tgataatatt
    31861 ttagataaag ctgaattaca acaaaagctc tcagaaaaat tacaacaact taacatgatt
    31921 taaaggaggt acgacctatg caagcaatcc ttaattttat agtcgatatt ttaagtcaac
    31981 cagccattct tgttgcactg attgccttta taggtttaat cgttcagaaa aaacctgccg
    32041 caacgatcac ttcaggaacc attaaaacga tattaggctt cttaatttta agtgcaggtg
    32101 ctgatgtcgt cgttcgatct cttgaaccat tcggcaaaat attccaacac gcatttggtg
    32161 tgcaaggtat cgtacctaac aacgaagcta tcgtctcact agccttaaaa gattttggaa
    32221 caacagctgc actcatcatg gtctgtggca tgattgttaa tattttaatt gcccgcttca
    32281 ctaatttaaa atatatcttt ttaacaggtc atcatacatt ttacatggct gcgtttttag
    32341 caatcatttt aacagtcagt catattaaag gctggctaac gattgttatc ggcgcactcg
    32401 tattaggatt aatcatggca gtattacctg cattactcca acctacgatg cgaaaaatta
    32461 cagggaatga ccaagtagct ttaggtcatt ttggctcaat cagttacttt gccgcaggtg
    32521 ctgtaggtca attattcaaa ggtaagtcta aatcaacgga agagattaaa tttccaaaag
    32581 gcttaagttt cttacgagaa agtacaatta gtatctcgat tacgatggca ttactttact
    32641 tcatcgcatg cttatttgcg ggcgttagtt atgtacacga atctattagt gatggtcaaa
    32701 actttattgt cttttcatta attcaaggtg tgacatttgc tgctggtgta tttattattt
    32761 taacgggcgt tcgtttaatc ttagctgaaa tcgtcccagc atttaaagga atttctgaaa
    32821 agcttgtacc aaattctaaa cctgcattag actgccctat tgtgttccct tatgcacaaa
    32881 atgcagtatt aattggattc tttgtcagct ttattacagg tgtcatcggt atgtttatct
    32941 tattcttatt tggtggcgtc gtcattttac ctggcgtagt tgcacacttc ttcttaggtg
    33001 caacggctgc tgtattcggt aatgcaagag gcggtattaa aggtgctatt gctggcgccg
    33061 ctctaaatgg tatcctaatc acgtttttac cattattatt cttgccattt ttaggcgaat
    33121 taggtggtgc tgcaacaaca ttctcagata cagacttttt agctgtcggt atcgtgttcg
    33181 gtaacgcagt aaaatatatg ggattatttg gtgcgattct atttattatt atcgtaggtg
    33241 cgacaacaat tttattaaaa ggccgtcaaa aagaacagca atagtgttaa cgtagaaata
    33301 taaaacaccg tcacatattg agtgaatgcc ccttttatca agaggaaagc cacttactta
    33361 tggacggtgt tttgtattat attaaatgat acttagccat actatcgaca gctgctaaaa
    33421 ttgcttcttc ttgtgtcgca atcggttccc aaccaagtaa tgtttttgca cgttcgttac
    33481 ttacattgcg gttcatatct aataaaagtt taccttcttt agcttgatga ttaaatttag
    33541 cacctagact caaaataaag tctggtaatt ttttagtaga aactttttga gctatttcag
    33601 gtctcttttc tttaattaat tttgcaattt ccaacaaatt aatttgtcca tcagccgtcg
    33661 caataaatcg cttgccatta gcttgttcat ttgtcattgc caaaatgtgc agttcagcta
    33721 cgtctctcac atcaacaaca tttaacggaa tttgcggtac acgtttcatt gaaccattca
    33781 ataaattttc taataaatga aagcttcctg aaacgtgtgc atctaatgat ggcccaaaaa
    33841 ttgcaactgg attgattgtg gcaaattcta ctgttgtatt ttcattctca acaaaatccc
    33901 acgctgcctt ttcagctaac aattttgatt tttcatatac tgataagcct ggttcatctt
    33961 catttgtcca atgactttca tttgtgattg aatttttatc tttattacta aaaccaactg
    34021 caccaaagtt tgcagtcatt accacacgtt taacacccgc atgttctgca gctcttaaaa
    34081 tacgttgtat accttcaatt gcaggcttcg ccatcacttc tgcatcgtct gttttaccga
    34141 aaaacaccgg agatgctaca ctcaagacat acttgcaatc tttcattgct tcatcccaat
    34201 gttcatcttg tgataaatcc gcttcgacaa acattaatcg ctctgtggaa atgccattgt
    34261 cttgcattgt tttaattact ttatcagctt tacttaaatc acgtatcgtc gtttgtacgt
    34321 cataaccttg ttctaataat cgtgaaataa ttcgcatgcc aacaaaccct gtaccaccgg
    34381 ttgctaatac tttattattc atcacataat ctcctctttg tgtctattga ttactcatta
    34441 tagcagtcat tatattttgt atatatcaac agacttatat tgcttaatat tctatatgaa
    34501 ttataaaagt aaacatcatt atattcctta aaatattaaa ctcatctatt ttcaaatacc
    34561 caatcattta gaaacaaaaa ccatatatat atcatacttt gaatagttaa aggcaataat
    34621 ttccactcat atgttcaacc ttcaccaaac actattgtca ccttttcaag agcaagcaaa
    34681 cacttccaat gattcagcat acataaaata acaatattaa cttaaaatca tatccactaa
    34741 tgcttctacc gtaacattgc taaaataata aacgatgtct aattgcttta actgatgcgt
    34801 taaatcttct cttgtcattt ttgttccttg taatgcttct tccacatctt ttatatcacc
    34861 ttgtccaaag aaatccccat aaatacgaca atctgcgata cgattttgtt caacagaaat
    34921 tgttatgtct accgtacctg aagataatct ttcacttcga ttgtattcat atttgggtga
    34981 cttgccataa ttccaatccc aatttttata ctttttatca gctaattcat caatcgcttc
    35041 ccaatctgca tccgttaatt catatcgttt cgcctcttta atgtcatcga ttcccaaaat
    35101 ctgcgtcacc attaaatttt taaattcttc aatggttata tcacgatact tttcatccag
    35161 tgcttctcta agatgaccaa cacgtgcgcg cacagattta atccctttcg atgcaatctt
    35221 tttgcgatta ggctttaaca ctttatccat tgcttcataa ttaacatcaa gtaatagcga
    35281 ataaccgcca taaatacgat tattcattaa tgtcattgcg gcgcctgaca cttttttacc
    35341 attcaatgtt aaatcatttc taccgctttg taccacatct gttgcaccta atgtatgcaa
    35401 cgcctttata gctggttgat aaaatcgttg aaaatcacca taaattgaag tgtcttgttc
    35461 taaaatacaa cacatattaa cggcaccttt atcaacatac acagcgccac caccagtatc
    35521 tcggcgaact acttgaatac tgtgtttatc tatataatct tgattcactt ctatagcagt
    35581 attttgaaaa cgtccaattt ccacttttgg atcacaataa taagggaata aaatatcttc
    35641 atctaaaaag atatgctggt taacataaac ttgcatagcg agtgcaatcg caccatcagt
    35701 aatatattct ccatttctaa tcggttctat taagtacatc ttctgtctct ccaatgttgt
    35761 tatttttcgt tgtgctgtcg ttccggagtg ctgctgtaat caatgttgag atatcctcag
    35821 ttaaatgtat ggtacgttct tgaattgaat tcggaatgcg atatgccttt ttattcatcg
    35881 tcatataaag ggcattttca tttttacgtg tcatacgctg aaaaggatgc ttcacaaatt
    35941 gtggtgtagt ataaccaatt ccaatttcca aatacaacac tttatcatct tgatgttgct
    36001 ctagaaaagc attataacga tgtagttgcg catgaaattc agcatcttca accatcccaa
    36061 cttccgcttt acgtttattc acttccattg gggcatcaca ttttggacat cttggaatca
    36121 tctcccaagg tataagcata tcttgttgcg caacaaccat tttacgaatt aaatcatcat
    36181 tgcgatacgt ttgagcatga caatgctgac tacactgttg cagtatatac tccccttgta
    36241 tatgaaatac atgagtcata tcataatcag ctacatcgaa agcattatct gcattcgtag
    36301 ttataatgtg gtactgttta ccttccacca aggattttaa agcgaggtaa gactgaccta
    36361 caggttgatc taaatagttt aatgtaataa aacgactctc aaatgcccaa tactcttgcc
    36421 aactgccata aggatgtaaa ctcgcttgca acatatcaaa gaagcgatat ttttcaataa
    36481 aatctgggaa attttccgta aaacgctctc ctacatatgt aaatccgtca gatgcagaca
    36541 tgcctgcacc aattccaatc actatcgcat ctgcttcatc aatcgcagta cgcaatactt
    36601 cagcctgctt tgtcttgtca tccattaaca gagacattgc attccactta ctactctgca
    36661 tcacggttaa atgcctcctt atacagttgt aaatccttat ctgtaaatac attgaacacg
    36721 actttcaatg ttgaatttgt ttctttgaga tagctttcta ctgttcgaac agcaatttct
    36781 gctgcttcat cttgaggaaa agcaaataca cctgtagata tacagcaaaa agcgacatga
    36841 tttaaactat gttgatcagc caatttaaga cagctaagat aacatttagc taacaagtcc
    36901 tgattcatct ttgaaacagg caatcgacgt atttgcggac caaccgtatg aattatatac
    36961 tttgctgaca aattatatcc acgtgttatt ttggctttac ctacaccttc attgcgccct
    37021 tgttgtcgaa tgatctctgc acaatcaagt cgaacttgaa cacccgcttt tgtatgaata
    37081 atattatcaa tgcagtcatg attagcttgc atacatccta gaaaacgact atttgcagca
    37141 ttaacaatag catcgatttt taacgtcgtg atatcacctt gccaaacaaa gatattgtca
    37201 ccttttatcg gcttcaaatc cttcaaatcc gtaacatgtt gacgattcaa atcacttaac
    37261 atagcatctt gtacagctaa atattcatca cttacaggta atgccggtct gacatttgct
    37321 aagcctcgat ataattccca caaatcttca aatgaagatg gcattaccaa tacgtcattg
    37381 tcatttctct ctcgatgcat atcattgatt aaatattcaa gtctcgcttt atttgatttt
    37441 aacgtttcca tttaagcctc cggtaatgct aggaatgctt ctttatcgac atcatccaat
    37501 ttgaacaacc aattttcttc tggtttttca gagtttaaaa ttgtcggttc ttcttccgct
    37561 tttgtatttc gctcaataat cgttcctgac aatggcgttt gcacatcaat gaccgttttc
    37621 gatgcttcga tactcacaat ttcatcatcc actttaactt catctggact tacgaattca
    37681 acataaccta ctgtcccaat atcatcttgc aattcaggtg tcatactaaa cacatacaaa
    37741 tctcctactt tttctaccca taaataattg gctaactttt tcatcttatc ttcatccttt
    37801 ctcttctatt taaaatgcta caaataaatt tccgcgagta attttaatgt tttacaacgc
    37861 tgttcaatac ctggaataag cggtgctact aacacctcat caacttcaaa cgtagcaatg
    37921 aaatcatcta attgtgcttt aaccttttct tgtgtacctg caatgatgcg tgcttgatgt
    37981 gcttgaatca tctctttgtc tcgatcatta agcttatact tttgtgctgt gtctactgaa
    38041 ggaaaatctt caaattctgc aaattgtaat ttacctaata accaaacatc taaggcatgt
    38101 tgtaatgctg ctacttccgc ttcgttatca gctacaatga caaatacaga tgccatcacc
    38161 tttgcgtcca ttttaatcgt tgatgcttgg aaatgttttt tgtaaatatc aatgttatcc
    38221 ttcgctgcat gtatcgcatt tatatctggt agcaaaaatg ttccaacaga aagccctata
    38281 cctagttcgg cagctatttt ggcagatgtt gcgctactac ttaataacca catttctgga
    38341 aaatgatcaa tgtgtggttg gacacctaac gtatgcgcac ttggtttatc ctttattgta
    38401 agataatcac gtaataacga aatcgattca tcgtaactat catatgtagg atttattcca
    38461 tctaaagctt gctttaccat agtagtacct ggattattac caatacctaa atcaatacga
    38521 tttggatata acgctgccat cattctaaaa tgctcagcaa ttttataagg tcgatagtgc
    38581 ggcagcatca caccaccaga gccaactcgt atgtgatttg tctgcgccaa tgtatgcatc
    38641 atcaaaagtt ctggactact acacgcaaac gctggtacat tatgatgttc cgtaaaccaa
    38701 attcgcttaa agccaagtcg atctgctaat tttgcaagtg tcactgaatc ttgcaatgcc
    38761 ttttgtgcat ccttaccttc atctattaag gcatagtcta atacgcttaa tttaaccaat
    38821 ccgtcatctc caaacttatc ctgtcatgtc aaaccgacat aacattttag cgttcttaat
    38881 accattccct cttcatatac ccacgtatat gataacgttt ttcaattaac ttttatatct
    38941 tttcgccttt atttttcttt ttcatattca tattttcaga cctacaaatg ccattcatta
    39001 gtttaatttt aaaaatactt tccatgtacc aattctattc tatcaagact gttcatcatt
    39061 gattattcat tctttccatg gtactttgaa aacaatattg aatagaaaag gaatcatagt
    39121 atgtaccgat acaaaccact tttacaatca atccacttac ctaatggcat caaaatttcg
    39181 aatcgctttg tattatcacc aatgaccgta aacacatcaa caaaagaagg ctatattaca
    39241 aaagaagact tggcttatgc tgcacgtcgc tctaattcag ctggaatgca agtcaccggc
    39301 gctgcttaca ttgaacctta tggtcaattg tttgaatatg gctttaatat tgatcatgat
    39361 gcttgtatcc ctggattaac taacatggca tctacgatga aacaacatgg tagcctcgct
    39421 attatccagc tagcgcatgc tggtcgtttt tcaaatcaag ccattttaaa ttttggaaag
    39481 gtgtatggac caagccctat gacattgcat tcaccgattg aacacgatgt tatcgccatg
    39541 tctcatgaga aaatcaatag catcattcag cagtatcgcg atgctacatt acgcgcgatt
    39601 aaagcaggtt ttgatggtgt ggaaatttcg attgcacaac gtttgctgat tcaaacattt
    39661 ttctcaactt tttcgaataa acgtacagac cattacggtg ccgactcatt aaaaaatcgt
    39721 gcaagattat gtttagaagt tatgcgtgcg gtacaagaag tgattgataa agaagcacct
    39781 gacaatttta ttttaggatt tcgagcaacg ccagaagaaa ccagaggcag tgatttagga
    39841 tataccattg acgagttcaa ccaattaatc aattgggtca tggacgtttc aaacatccaa
    39901 tacttagcaa tcgctagctg gggtcgacat atttatcaaa atacgtctcg tacaccaggt
    39961 gatcatttcg gtcgaccagt taaccaaatt gtctatgaac atttagcagg tcgtatccct
    40021 ttaattgcaa gtggcgggat taattctcca gagtctgctt tagatgcttt acaacatgca
    40081 gatatggtcg gcatgtcttc gccttttgtc acagaacctg actttgtgca taaacttgct
    40141 gaacagcgac cacatgatat caatcttgaa ttttctatgg cagatttaga agatttagcc
    40201 attccacatg ctgcttttaa agatattgtt aaaatgatgg attatgggga aggtctaaag
    40261 aagcataccc gtgacgcttt acgtcaattg gaacagaatt atcatgactc atcgtcttaa
    40321 gactattttt catggaaatt taagacagtg ttataatgac atcaatatca aaaataagga
    40381 gcctattaaa tcgtgactga aattaaagat aaagtaatta ctaaagatgc atttgcatta
    40441 ccttacacaa ttattaaagc aaaaaatcaa ccaacaaaag gcgctattgt ctacatccac
    40501 ggtggtggat taatgttcgg taaagctaac gatttatccc cacagtatat cgatatcctt
    40561 acggaacatt acgatttaat tcaactaagt tatcgtttat taccagaagt aagtctcgat
    40621 tgcattattg aggatgtcta cgcatcattc gatgcgattc aatcacaata ttctaattgc
    40681 cctattttta cctttggcag atcatcaggt gcatatttaa gtctattaat tgcacgagac
    40741 agagatattg atggtgttat tgatttttat ggttatagtc gcatcaacac tgaacctttt
    40801 aaaacaacta atagttatta cgcaaaaata gctcaaagcg taaacgaaac aatgattgca
    40861 caactcactt caccaacacc ggttgttcaa gatcaaatcg cacaacgttt tttaatctac
    40921 gtttatgcac gtggcacagg taaatggata aatatgatta acattgctga ttatactaat
    40981 tcaaaatata atattgcacc agatgaactt aaaacacttc cacctgtttt cattgcgcat
    41041 tgtaatggcg attatgatgt tcccgttgag gaaagcgaac atatcatgaa tcatgttcca
    41101 cattcaacat ttgaacgcgt gaataaaaac gagcatgatt ttgatcgtag acctaatgac
    41161 gaagcaatca ctatttatcg taaagttgtt gatttcttaa acgctataac aatgatgtaa
    41221 tatatcatta ttttaaaaaa ctcaatttat catcatgata aacagttatt cgatcaacca
    41281 tattattcat acaatttatt aagctcataa acagatacgc ctgtcccttt agttttcgct
    41341 aatgagacag gcgctttatt tcactttact atttttaaat tcaacactgt gatgctactt
    41401 atatctgatg tgccgtgata acagcacgat ttacatagca tattaaattc agaagatgaa
    41461 tttaacttgc tttcaattgt gttcctttac tttcagtcgc ttcaacacgc aacaagtcat
    41521 ttataatacc tgtatagaag ttggcaagtt ctgcaccttt acgtttgaaa tccaggaagt
    41581 ccacaccgat aaaatcgaca tgatcccatc cttgtatgat tggtttaact tgccagatac
    41641 ctctgcgtgt ggcaggttca tcattcgtaa cattaacaaa tggttgattg gacggatgta
    41701 atgacgaaat cactggtacg acaccatcat ttttacgcca ttcttctctt gcatcatgac
    41761 caataattct actcgttgta gccattaaga aaaatgtacc taaatcagga ttttcataac
    41821 ctaatggacc agtatgagat gatacacctg tataagtcgt atacgtaata ttaggattca
    41881 tacttgtcat gttgttcaat tttgcagagc catctaacgt taaatcatag gcagcattgt
    41941 cgtctgatgt ccaaattttg cttttactaa cgcgttttat atagtcaatg taactctcat
    42001 ttggtaattg tttaaagccc cattgcgtta atcctaaatc gatattcgaa tacttgttac
    42061 ccataaatcg atttaaagcg aacatgattt ttctaacagc ttctgtattt ccaaacttat
    42121 cagctgcttg tgaaccatta tgtggtgttg ctaatgttgt gattgatgca accatattgt
    42181 tatgaccacc agtgaataat ggtgatattt ctccaccatg cgctttatga taggcaattt
    42241 cttctttgtt accatttctt aaaaactctt ccattaaacg aattgtttga ccacccatac
    42301 tatgccctac aagatgtacc tttttaccag gttcccaatt aggcatgatt cctttataag
    42361 tcttaccata gcgctcatgt ccgtatttag ctgcatgtgc tgcgccataa tctacgcgac
    42421 cacctttaat gtaataataa agttctacag cgcgatcata gttactacca aatgcactta
    42481 cacttgcttg atgtacatta tagccttgct ttctcaattc ttcgataact ttaaatttat
    42541 ttccacccca ataatttgga tataaagcag gtgcattatc gcctactaat cctaaaaatc
    42601 catgtacaaa aacaactgga tatttgttaa gtggttgtac ttgattcgct ttaagtggtc
    42661 gaaccttttt agcatctgct gctgcatttg tagtcgaacg tgttttaggc gcttgaagcg
    42721 catctttgat tagttgttta ttatcagtat ttttatctgt cggtttctct ggggaatttt
    42781 gaccatcgat atgtttactt aaatcgccaa cttgtggttt ctgttcactt tggcgaacag
    42841 tatcatcttg ctttgcatct atgatttgat gcgcaggttg atgtgtatct tgttgatttg
    42901 gatgtttgtc cgtggttgca tcttgtgctt ttgttgattt aggtgcagtt ttatcattag
    42961 acgggggtgt agttgaagtt ggtgctgctt tatcatgatt gggtattacc ttttgtgccg
    43021 gaatggatgg ttgtgattta tcaacatcat tcgattgtga cgttgtcgat tgtgatccat
    43081 tacgttcagc atgatgggta ttttgaacct gatcattttg acgttcagta ttattagtcg
    43141 cattattttg gtgtgactgt ttagaacctt gtcgttcaga atcttgcttt acttgattat
    43201 cattttgcat tctgattgtt ttactatttt gtgttgattg atgcttcgat gattgaagcg
    43261 tatctttacc ttctgtcact gttccacttt taccattctc tttatgcata tcgtctaatt
    43321 gctttcctga ctgcatttga tgtgacgtta tcgcattccc ttgttctccc ggttgattta
    43381 gtgtttcttt ttgtgccgct gcattagttg atgttttttc cgaggcttgt gcttcatgtg
    43441 atgacacaac aaacattgta gccgctaaca ctgacaccac gcctattgaa tactttctaa
    43501 tactatactt tctttcttct tgtcctctta acatataatc acctcaaaag tatttttttg
    43561 accccctctt gagaatgaaa aacaactgca ccttcaatat aacatgacat atattatttt
    43621 ttgattaaga ctacaaaatt aataaaaatt taataatacg atattaaatc gatactaatc
    43681 atttacaaat atgtgtattt ccaaataatt tataaacact tttagttgtg gtgttgaatc
    43741 aacacaacaa catttaagat aaatatttta ttaagtaata tagtttcaat ctatatataa
    43801 ttagttaatt tgtattggta tgaaaataat tcaaactata caattatagt aagttttcag
    43861 atttttctaa attaaaataa tgattcatct cattaagggg aagatgcatc atacagtttt
    43921 gtgtattcat ttaaacaaag gggttgcttt aatgaatcaa tatcattcta atgcacaaca
    43981 accaagtgca tggcgttttt ttgtctatag tttagtgggc atactatgtt tctttattcc
    44041 ttttacgatt aatggtaaca acactatttt cgtcgatcat gttcatctag ccattcgctc
    44101 aatcataggt ccacttatgc cctatgttgc actgattatg attttaattg gtacagcgtt
    44161 accaatagtg agacgtactt ttatgacttc aatcacaaac ttggtcatta cattatttaa
    44221 agttgcaggt gcaatgatcg gtataatgta tgtatttaaa atcggtccat caatactatt
    44281 taaagctaac tatggtccgt ttttgtttga aaaattaatg atgccattaa gtatcttaat
    44341 tccagtaggt gcaattgcgc tttctttatt agtgggctat ggcttattag aatttgtcgg
    44401 tgtttatatg gagcctatta tgagacctat ttttaaaaca ccaggaaaat ccgctgtcga
    44461 tgcagtggct tcgtttgtcg gcagttattc cttaggatta ttgattacta atcgtgtcta
    44521 taagcaaggg atgtacaaca aacgagaagc cacgattatt gcgactggct tttcaacagt
    44581 ttcagcaact tttatgatta tcgttgctaa aactttagga ttaatgccgc attggaattt
    44641 atacttttgg ataactttag tcatcacatt tgtcgtgact gcaattactg catggctacc
    44701 gccaatcagc aatgaatcaa cagaatatta taacggacaa gaaggagaac aagaagttgc
    44761 tattgaagga agcagactga aaactgcata tgcagaggcg atgaaacaaa atgcattaac
    44821 accatctctc gtgaagaacg tttgggacaa tttgaaagac ggtttagaaa tgactgttgg
    44881 tattttacct tctatattat cgattggttt tttaggactg attgtagcga actatacacc
    44941 attcattgat tggcttggct atatcttcta tccatttatt tatattttcc caattgctga
    45001 tcaggcttta ctagcaaaag cgtcagcgat ttctattgta gagatgtttc taccatcttt
    45061 gttagtaact aaagctgcaa tgagtactaa atttgtcgtc ggtgtagtaa gcgtatcagc
    45121 cattatcttt ttctcagcat tagtgccatg tatactagca actgaaatta aaatacctgt
    45181 ctggaaactc atcatcattt ggtttttacg cgtggcgttg tcgctattaa tcaccatccc
    45241 cgtcgcttta cttatttttg gataaagttg aaaataaaat cactatcata aacaaacaaa
    45301 acgagatgaa cccattacat tcattcagcc acctaagata attaggtgcc tttaattaat
    45361 gatggtcatc tcgttttttt atcgttatca tttaatcttc cataatttga acaaaacgtt
    45421 tcgtaatttc ttttggtcgt gttatcgcac caccaacgac tgaacaatga acgcctaagt
    45481 ccatcacacg tttatacata tccggtgtaa tgacattacc ttccgcaata acttttgcat
    45541 caacactttg tagtacatct tttaaaaatt ggaagtcatt ttgataaagt aattgtcctt
    45601 gcgtataact agtatagcca tgtaacgtcg tgccaatata atcaaagcca agtcgtgcgg
    45661 catttttagc ttcttcaacg gtcgcgatat cagccatgat ttcaacgttc ggtgcatgtg
    45721 ttctaatata tgatactaat tcgtctaacg tttctttcgg acgttgctgt aacgttgcat
    45781 ccaatgcaat gacttcacat tggctttcta tcagttcatc aacttctttt gacgttgcag
    45841 taatgaaaac atctgagtga tcatagtcac gtttcacaat gccaataact ggtaaatcta
    45901 ccgtttcttt aattgctaaa atgtcttcct tagtatttgc gcgaatacca acagcaccac
    45961 cttcatacgc agctaatgcc attttcgaca taataaaaga tgaatgcaat ggttcatctg
    46021 gtagtgcctg acaagatact attaatccat gtggtaacat tattcctcac tccataagtc
    46081 gttttttcac gtaaagtcat ttacaatata tcattaagat aatttttatc aatgcttttt
    46141 actatcttga aaatattttt catatataat agtaatcatt aagatgaagg ggtgacacaa
    46201 tgaaatttga gaatcgtgtt caacgttatc aacatttatt tacaaaaact gataaacgca
    46261 tcgtcaatta tataagacaa aatggttata gcgatgcctt ttcaacgatt aactccttag
    46321 cccatgcgat tggtacatca ccagcaacga tgacacgctt tagtcataag ctggattatg
    46381 agaatttcca agatttaaaa tttaatattc aacaagaaat gacagagaca gttattgaaa
    46441 atagcccaat tattcaaaga attcataaat atcatcaaca aatcattcaa caaactggtg
    46501 aatttattga taacgacatt atccaaacct ttatcgacaa acttcaatca agtcgacata
    46561 tactctttgc tggactaggt agctctggtt tatcagcgac tgaattttat tatcgtatga
    46621 ttcgtatggg gctaaaaggt aatgttacta ccgattcaca tttaatgaaa atatcggcat
    46681 ccctactatc tcattcggat atgtttattg ctatgtcaaa tagtggtaat acttcggaat
    46741 taatttctgc agcggaagtc gccaaatctc atggtgcata tgtcgttgcc atcacaaatt
    46801 ttgaaggtag taaacttaca gattgtgcag atttagtact tttaacaacg gatcaatcgc
    46861 gtaataccga ccatcaattt atcaacacac aaattgcgac actcttttta atcgatatcg
    46921 tgagttatca tttattagaa aatacgaatc tgagtcaaac ttatcaacat actaaatcta
    46981 ttatcctaga caacaaataa ctagtagtat attcaaatcc acctgcaact gtagacaatc
    47041 atcgtgattg tcccaacgag ttgctaggtg cttttttaat ttcaatacca aatatttttt
    47101 tacattttct aaaattttaa tatctcagct tcttcaattt aatttgcccg tggcacactg
    47161 ttggttgata tagtttagat tggtcctaga tagagtgtga tgtgtggaac taacaacagc
    47221 tctcatctct cgtaacggtt tcaaattcta ttttagaatg actccttctt tcaactatca
    47281 ttgcaaacag ccatataatg ctgcatcatt tttactctta gtcgtttgta ttggtgcata
    47341 aacatagtct tttggtaaat agtgtgcaac tttcggctcg atatatttaa tgagattatc
    47401 tccttgttca gatataccgc ccccaattaa tataagccct ggatcataca tgacctgtat
    47461 ttgggcaatc ccttctgcta catcttctgc ccactcattc aatatttgtt ttgcaatatc
    47521 atcaccttct tcagctgctt caaacaatac tggcacatgt gtgcttctcg taaatcctcc
    47581 ggcaatcatg cgctttttca atgcactcgt tgcagcacgt tgctcaaacg ttgtattttc
    47641 agttggacga tacaataaat acccaacttc atttgcctta tgaagctcac cattatcaat
    47701 atgaccttga ttattcttgt acgcaccccc aatgcctgta ccaagcgtca tacaaaagat
    47761 ccgttctgct tgatattgat gtaatttcaa ttcgcctagt aatgcagcgt ttacatcatt
    47821 ttttactttg acataaggag acagtgattt taataatcgc ttaaaattag tacctttata
    47881 attcggaatg gttggccctg cgtatacaat ttcgcctttt tgttcatcaa caacgcctgc
    47941 tgatgaaata cctatgacag gttggatcaa ctgatattgc ttcatatatc ctgttacaat
    48001 ctcatatact ttgtcagtaa taagctcact tttgttgtcc ggcgttgata tttgttgata
    48061 gtcaaacata ttcaattgct tatcaataac tgccgattta atttgagtgc ctccaatatc
    48121 gattgcgatg taatacacct tactcactcc atttctattg attttgaaaa acattttcat
    48181 taattataca taaatatgac tacaattttc aaaaagatat tgttacattt tatgatagcg
    48241 tttacaatta tagctgtaaa ctatattatc aaatgatttt tggagggact tcataatgaa
    48301 caaagattta aaaggtttat atgcagcgtt actcgttcct tttgatgaaa atggtcaagt
    48361 aaatgaacaa ggacttaaac aaattgctca aaatgccatt gaaactgaag aattagacgg
    48421 tctttatgta aatggtagct caggcgaaaa ctttttatta aatacagagc agaagaagca
    48481 agttttcaag gttgccaaag aggcagttgg ggataaagtg aaattgattg ctcaagtagg
    48541 ttcgttagat ttaaatgaag ccattgaact tggaaaatat gccacagaac tcggttatga
    48601 tgcactttct gccgtaacac cattctacta tccatttact tttgaagaaa ttagagatta
    48661 ctatttcgat attatcgaag caactcagaa caatatgatt atttatgcaa taccagattt
    48721 aacaggtgtg aatatttcta ttgaacaatt cagcgagcta tttaatcatg aaaaaattgt
    48781 tggtgttaaa tatacagcgc caaacttctt cctactcgaa cgtattagaa aagcattccc
    48841 agacaaatta atcttatctg gctttgatga aatgttagtt caagctacga tttctggcgt
    48901 agatggtgca attggttcta catataacgt taatggtcgc cgtgcaagaa aaatcttcga
    48961 cttagcacga caaggtcaaa ttcaagaagc ttaccaactg caacatgatt caaacgacat
    49021 tatcgaaaca gtgttatcaa tggggattta tccaacattg aaagaaatat tacgtcatcg
    49081 tggcattgat gcaggattac cgaaaagacc tttcaaacct tttaacgaag cacatcgaca
    49141 aacattagat caactcattg caaaatacga tttatagaca tcaaagaatc aatacattgt
    49201 aaaggggatg ttgcccatga aagaagttgg atttggcaca ctaaactggg ttgccgttat
    49261 catttatcta ctagctatgt tgttcattgg cgtttatttt accaagcgcg cgagccaaag
    49321 taccaatagt ttctttaccg caagtggtcg cttgccatct tgggtagttg gcttttcaat
    49381 ttatgctact acgttaagtg cgattacatt tatgtcgaca ccagagaaag catttttaac
    49441 agattggtca tatatcgctg gtaacattgc tatcgtcgca attattccat tacttattta
    49501 tttctatgtc cctttcttta aaaagttaaa ggtaacatct gcatatgaat atttagaagc
    49561 tagatttggc cctagcatac gtgtcattgg ctcattatta tttgtcgttt accatttagg
    49621 gcgtgttgca attgttatct acttaccaac attagcaatc acatctgtat cagacatgaa
    49681 cccttatatc gttgcatcac tcgttggttt actatgtatt ttatatacat ttttaggtgg
    49741 tttcgaaggt gtggtttgga gtgatttcat tcaaggcgtc attttattag gcggcgcttt
    49801 agttattatt attctaggtg ttgtgaacat taaaggcggt ttcggcactg tctttgcaga
    49861 tgcgattgag cacaaaaaat taattagtgc agacaattgg aaactaaata ctgcggcagc
    49921 tgccattcca attattttcc taggaaatat tttcaacaac ttgtatcaat acacagcgag
    49981 tcaagacgtc gtgcagcgtt atcaagcttc tgatagttta aaagaaacaa ataaatcgtt
    50041 atggacaaat ggtatcctag ctttaatttc agcaccctta ttttatggta tgggtacaat
    50101 gctgtattca ttttatacac atgaagctgt tttaccaaaa ggcttcaata catcatctgt
    50161 agtgccatat ttcattttga ctgagatgcc accatttgta gcaggattac ttattgcagc
    50221 cattttcgcc gctgcacagt ctaccatttc atctagttta aattctatat ctgcttgtat
    50281 ttcaatcgac attaagcaac gcttcttcgg aaaaggtagc gagcgacacg aagttaactt
    50341 tgcacgtttc attattatca ttgcaggtat tttcggtttt ggaatgtcac tatacttaat
    50401 tgcttctaat tcaaatgact tatgggattt attcttgttt gtgactggat tattcggcgt
    50461 tccattggct ggtgtatttg cagttggtat tttcactaaa cgtacgaata cattcggtgt
    50521 tatttgtgga ttaatattgg gtatcatctt tgcttatgtc tataatggtg ttggcaaagg
    50581 taactcacct ttctatgtat ctaccatttc atttacagtt gcttttgtct ttgcttatat
    50641 acttagcttc attgtccctt caaaacataa aaaagatata acgggattaa caatttttga
    50701 aaaagataaa ccatcaacat acatttcaaa aacggctacg aaaaagtaga ttgttatgat
    50761 aaaaccccgt cactaagtta tgatgcgctg ttgcgccaac ttggtgacgg ggtttagctt
    50821 tgccatgaat ttaatttagg tacttcgatt catttacaat actaagccaa tgattgatcc
    50881 tgaaatgatt gaagctagag ttgaaccaag tagcaacctc attgcaaagg atgcaacttt
    50941 ttctccttgt ttatcactaa tgcctttaat tgaacctacg atgataccaa ccgtaccaaa
    51001 attagcgaag cttactaagt aaactgaaat gataccttgt gttcgagctg atacatcacc
    51061 caggacattt ttaaaatcaa gcattgctac aaactcattt gtaattaatt tagtcgccat
    51121 taaagagcca gctggaacag cttcgctcca tggaatcccc attaagaatg cgattggtgc
    51181 aaacacatag ccaataagct gtttaaagtt caaaccaaca ctaccaaaca tgatattaat
    51241 tgcttccatt aatgaaataa atgctaacaa cattacggct actacaacag cgattttaaa
    51301 cccatccatc gcactatcac caatcatttg gaaaaaggca actttcttag gttttcctgt
    51361 ttttccattc aatgttttag tttctgtgga tttcgttaag ttatcaattt caacatcagt
    51421 atcatcagat ttatagggat tgattacact ggcgatgata agcgcactaa aaatatttaa
    51481 cattactgct gtaactacga acttgggttc aatcatctgc atatatgaac ctagcattgc
    51541 catactaaca gcactcatac cagacgtcgc aattgtatat aatttcgctc tagataatct
    51601 tggaataata tcttttattg ttaaatatac ttctggttgc ccaaacattg ctgttgaaat
    51661 agcaaaataa ctttctaagc gccccattct agttatttta ttaatagcga tacctacata
    51721 tttgataata aatggtaata ccttaatata attaaagatg cctattaata cagaaataaa
    51781 aactaatggc agtaatacgt ttaaaaagaa cgtaaagcca tttttatttt gtatatctcc
    51841 aaaaacaaaa tttatgcctg ctttactaat atttattagc ccttcaaaaa atgaacctag
    51901 tgcagttaaa attgtcaaac caatcgttgt gttcatcata aataacacga tcaacacttg
    51961 aataaatatc attattaacg tctttttgaa gtctattttt ttacgatcaa aactgaataa
    52021 aaacgcaaca aatagtgcaa atgctatccc tgtgatagcg aataaaatat tcatgtatca
    52081 tcacctacaa taatttatta acagcgacag caattttagc acccaacgcc gcattgtttt
    52141 caacaagttt tatatttgct gctaaacttt taccattcgt tttttctaca attttcccta
    52201 acaagaacgg tgtggcgtcc ttacctttaa taccttgatt ttccgcttca acaacagctt
    52261 catttatgat tgcctcaata tatgcttttg ataaggcatg ctcatatgga attggattag
    52321 caacaacaat gccaccttca agatttaact gctgttttgt taaatgaatg tcagcaagtc
    52381 gttctggcgt ttcaaccgaa cttgttaact taacaccgct ttcgcgagtg aagaatgctg
    52441 gcaattcatt cgtttgatat ccaataactg gaacgccttt tgtttctaaa tactccatcg
    52501 tcttaggtaa gtctaaaatt gatttggcac ctgcacagat aacagtgaca tttgttttag
    52561 acagttcttc taagtctgct gaaatgtcca tcgtatgttc tgcaccttta tggacgcccc
    52621 caatacctcc tgtaacaaaa aattgaatac cagccattgc agcacatatc atcgtcgtcg
    52681 ctacagtagt agcaccaaca cacttcatcg caataacttc tgctaaatcc cttctagata
    52741 ctttagcaac gtctttacta gttgccagta tttctaaatc ttcgctttct aaaccaattt
    52801 taattttgcc atctataatg gctatggttg ctggaatggc accattattc ctgataattt
    52861 gctctactgt tgttgccatt tcaacatttt gtgggtacgg cataccatgc gaaataattg
    52921 ttgattctaa tgctacaatc ggttgattgt tctcccgtgc ttgctgaact tctcgagaat
    52981 actcaatata cttttgtaaa tttgccattt ttataatcct ccatatcgtg ataaagttgc
    53041 tgttgatcta ggttttgcct aactgtatat ttcgtttcta tcgttttctt tgcgttaacc
    53101 ataccagcaa ttaatatatc ttcagtagac atcccattta accagctata cactactgca
    53161 gcacagaatg aatcgcctgc acctgtaaca tctttcacac tatttgatgg cataactgac
    53221 ttaatgattt cttcctcacc acttcgataa atgagttctt tcacgccatt tgtcacaata
    53281 acatttttaa cacctaaatc attccagcgt ttagcagcta tttttaaatc atcagtagat
    53341 tctattttta aatttaagta tgtttctgtt tcatctttat tcgtgataat ccaatcaata
    53401 gcatgtaatg aatcaggcat atttttcatt tttggggaag aaaccgtggt gataactaat
    53461 ttgatttgat gtttcgtggt ataggcacat aagaagttta atgcctcttt gcctaaattc
    53521 aaatctacaa taatgcactt agcctttttc aataagtgtg aacgcttaat taaaaattca
    53581 ggcgtaatgt agtcaaacac ttccatatct gctaagccat atgtcatgtc gccttcttta
    53641 ctaattaaag ctgtatatga acctgtactc gcattttcaa attgttgaac atgatccaaa
    53701 ttcataaatg gtgtggacaa tcgtttaatc atttcccatt cactatcttg tccactagca
    53761 gataaaaaag cgaccgtttc gccaagccta cctaagttct cagcaatatt tcttgctacg
    53821 ccaccaatag agcgtgttga cgttacagga tttgatgttt ctgcaactaa atttttatgc
    53881 acataaaact tacgatctac attcgctgcg ccaatacaaa caataggata atcttcattt
    53941 aaaacatatg cctttcccat aacatattcc ttttgtatta atcctgaaat aatgtttgct
    54001 acgctgggtc tagataatcc aattgcctca gcaagttcac gttgtgaaat aaacggatta
    54061 tctttaattc tttttaaaat ttctttctca gaatcgctca taacatccct cctcatattg
    54121 acttaaaata cttgaatcag tgaatttaca aaaatatcgt agcatgctgt tatttctgtt
    54181 gtaaacgctt acatttaccg ttatatcaat gatatttgtt tataaaatac gcataatttc
    54241 gtaaaaagta agttcattcc atcgtgtaaa aggtacttga gaaaatgata aatgtttata
    54301 cagtatgata tttgtcgaca ataaattatg acatttttat gtcttgtatt tttctataaa
    54361 aaaacgtgac tcattatcga ctgttacatc gactgaatca cgttactatg ttttatttgt
    54421 ttttactatt caatatgatg atgctatgcg tctactgcac ctcgattaat catgtcttgc
    54481 ttagtaacaa ttggatattt tttaaataca attgaaccaa gataacctgc aagcgcacct
    54541 acaatcgcca ttactacacc ataaatcaca attgtcgtcg gatgattaaa tccaaacata
    54601 actaaaaatc ctgcaatcgg tgtagctgta cctgtcgcat cgttaattaa acctgacata
    54661 gcaataatta aaccagcaat cgcaccacca aagaaatttg taatatagat tggaattgga
    54721 ttggctgata caatatctgc ttgtgataaa ggttcaatac ttactgcaat cgtagactta
    54781 cgatcaccta attttaagcg atggaatagc gtcccattca taaatgccga actaaatgct
    54841 gccatggcac caatagccat aggtacaccc gttaaaccta ataatgctgt caatgccatt
    54901 gaactcaatg gcgctgtgcc gacaaccgta ataataccac ctaaaatgat acccatgata
    54961 attggattcg tattcgtact actttggata atatcaccaa ttcgaatcaa tgtactattc
    55021 actactggcg ttaataatac tgcaataaga cgagctaatg gcgctaagat gacaatcgat
    55081 ccaattaagt cgacaccatc cggcacatat ttctctgtat atttcatcac atatccaatc
    55141 atataacccg caaagaaacc tggtaataaa tccataccac cgcacgcggc tgctataact
    55201 aatgcatata ctggtgacac accgatagct aatgctgtta atccagcggc agcgacacca
    55261 cctagacctc ccgcagcgtc tcctagctct tctaaaaatt taataccgaa tacttcgcca
    55321 ccaacatatt tattaaatgc ttccactaaa aatgttgcga ttgctgcatt agctaatgct
    55381 cccatggcac gcataccatt aggcgcttta tatgtaaata atgtaaaaat cactaagacc
    55441 aaaaataaaa ataaagtacc tatcaataaa tccataacca ttgaccccca ttacgataat
    55501 tttatattca atccccatat ttaaatattg aatacttaga ggccatttta ctacaaaaaa
    55561 tattatattt cacggaatat ttctgatttt cgataataat tattattaat tgaatgtgaa
    55621 agtttattca aataatacat aaacataaaa ataccggcac atgatgtacc ggcttaataa
    55681 aactattcta ctgtttcatt ttcaagatta aggacgccat ctttcatatg atatgaacga
    55741 tcacaatatg ctttaagtcg ttcatcatgt gtaacaataa tacatgcttt ctttctttgt
    55801 ttggcttgat cacgtagaat tttaatgact tcaatcgcat tttcagtatc taacgccgcg
    55861 gtaggttcat ccgctaaaat aatcgacgga ttcgtatata acgctttggc tatcgccaca
    55921 cgttgtttct gaccacctga aatttctgaa ggtaacttat taagcaatga agttagacct
    55981 aattgtgaca taagttgctg atagtcttca ttagacataa cattcttatt tttctttttc
    56041 aataatgtaa attgttgctt taccgttaaa aatggtacaa ggtttgtagc ttgtaaaata
    56101 aaacctattt cagacattct aacttttgcc aatgcttttt gcttcattgt cgtaatatct
    56161 tggttattga ttaaaatgtg cccagatgtc ggtgtttgta aagcacctgc catagttaga
    56221 aatgtacttt taccagagcc agaaggtcca accaatgcta taatatcacc tttatttatc
    56281 tcaaaatttg tatctttaac cgcttcaatg ttacgattcc catctttaaa tgactttgtt
    56341 acattttcaa atttcaacat ttgctacacc tcctacccaa tcgcctttaa cggatctatt
    56401 tttctaattg ttaaaatgga gaataaactt cctaatacag agacaatcat taacacaatt
    56461 gcaaatacga gcaatgttag tacatcgaat ttgacaggta ctgcatcagg taaaaatgca
    56521 cctgtaacgc ctgttaacag taagccaaat gccgtaccaa atagtgctaa tatgaccgtc
    56581 tgcgaaatta ccacattcgc caaatagcca ttcgtaaatc cttgagcttt taatatgcca
    56641 aataaactcg tcttttgtaa tgtcatgaca tataggaaaa tgcctataac tgtagctgaa
    56701 atgacaaata agaatgaaat cataaagttt aatgttaagt tctgtggttt ataacctggt
    56761 aaattttcaa taaagtcatt aatacttacc gcttcaagct cttggtttaa ttttttgtct
    56821 ttccaatttg tatcacgtac aacaactgca ttaattttat ctccagtcaa tctaggattg
    56881 atcttggcaa tggtagcgtc attcgtgaaa atgactgatg acgcattata ttttgcactt
    56941 tctgtaaaac ctacgatatg caatttttca tctgattgag atagtgataa tgtgtcacca
    57001 attttaaatc ctttattttt aagcgtttca tcagctaaca cttcattatc tttagtcgct
    57061 ttatgccctt ctattaaact cggaactaaa aatgatgact tttcaacacc gaacactaaa
    57121 acattgtctt tttgatggcc attagacaca atttcccctg tttgcttcaa agtagcttgc
    57181 ttcttgtatt tattttcaat atctttcttg ttaaaaacag attgttgcac agtttgattg
    57241 gcatctttat ttagaacaat ggcatctgct tgccacttat caatgccttc tttattcata
    57301 ttgataagac cattcgccaa tccagataat aaaaatagca agtaactaat catcgttaac
    57361 acaccaataa ttagtccaaa cttcaatttg ttgcgccgta tttcattcca agctaaaaac
    57421 atgcatttct ctccctacta ctatgattta aacattgttt atattcttag atgcacgtac
    57481 gtcgtgttgc gctctgtaat gttatacata cacttatcct tcattatacc cgaacttttt
    57541 atattaaaag caaatttatg gaaaatgcaa ttaattgtct attatttttg tacggtacat
    57601 ttaaaattaa ggatcaattt aaaaacgcct acatatacct ttaagtacat gtagacgtcc
    57661 aattcatata ttatttaact tcgcctgttt taggatcgaa ttgcttaata gcatttttac
    57721 gtaatttatc ttttgctttg tcacttgctt tatagttatt gttgtaaatc gtagcttccc
    57781 aactaccata cattgggtta gggaaaatga tatatttctt accgaaatcg tctttatgtt
    57841 tttcaattaa tgcttcacga gattcagctg tagcttcttt tggatctgta aagtctaata
    57901 aattatctcc aaatagcatg acaagtttat gatccttttg aaccatttgt ctgcgtgatt
    57961 ctttactctt atcatcttta ccttttagta aaatatgact cttcttagct tgagggatac
    58021 cttgttgttt taagttcttt tgtgttgcct ttaaatcttt ttctttatct ctatcagaaa
    58081 tatagtagat atcgacacct tttttgtcag catatttcaa gaattctttt gcgccataga
    58141 caggtttagc tttagcagct tgtacccatt catgccaacc ttctgggaaa ggtttattat
    58201 gtattgatgc atagccttga tatggagaat tatctaaaac tgtttcatct aaatccaaag
    58261 caatagctaa cttatgttta cctttattct ttttaatctc tttatctaac tgtgtctttg
    58321 cactgttata accttgtaaa tataatgctt ttgcttcagc tgaattttga taccaagcca
    58381 ctgccataat attttgatta ccaagattcg ccttttgtga tgctggtata gaagcttgtt
    58441 gcgtttgttg aacttcagca gaacttttgg caaacgctgt agaatttgtc gtttgtggtg
    58501 ctgaaactgt aaccgctacc gataatgatg ctattgcaat atactttgaa attttattca
    58561 tcttatcacc tcatgattaa tatttaaaat acagttaaaa ttataaatgc atttatttaa
    58621 tattgctata ctatgaaaag atatttaata ttatttcttg aaaagctaac aaatatgtga
    58681 acatttcata taagcatgat ttaatgggag acctatcaca tgagagagat ttgtgattta
    58741 tcacgtaata aacttaaatt catttgagtg actaggcctt cctatcagac atattcactc
    58801 atccacgtat cattatgtgt acagtgtgct atctcttatt tacctattgg aacaaccata
    58861 aactcatcca tagtttacct tttataaata gcagtcctca ctcatacaat ttctcataaa
    58921 aatcacaacg ctccaacgta tttccaactt actttcacct attttaattc ataaaaacga
    58981 cactttaatt gtcattatcc aataatagca agacgttatt attgcaatct tttttataaa
    59041 ataatagaat catagtattg tcatttaaag ataaagtaag aacgttttta tttttcagat
    59101 tttttaaatt attatgaata tctagtttta ggaaggaaat tacattgaaa aaacaagtta
    59161 ttatttcggg cctcatgtta ttttcactat tttttggagc cggaaattta atattcccgc
    59221 ccatgcttgg ccatacagcg ggtcaaaata tgtggattgg tatgctaggc tttgccctta
    59281 caggcatatt actccccttt attactgtta ttgttgttgc attttatgat gaaggtgttg
    59341 aaagtgtagg caatcgtata catccatggt tcgggtttat ttttgctgtc gtgatttaca
    59401 tgtctatcgg agcattttac ggtattccac gtgctgcaaa tgtcgcgtac gaaattggta
    59461 caagacacat tttacctgtg cataaccaat ggactttaat tatattcgca gcaatctttt
    59521 ttgccatcgt ttactggatt agtttaaatc catcgaaaat cgttgataat ttaggtaaat
    59581 tattaacacc gttattacta ttaatggtcg ctctattaag tattgctgtc attttcaacc
    59641 ctgaatctgc actaagtgca cctaaggata aatatataac acatcctttc atttcaggaa
    59701 gtttggaagg ctattttaca atggatcttg ttgctgcgtt agctttttcc gtagtcattg
    59761 tcaatggcta taagtttaaa ggcctcacag atcgcatgaa aattttaaaa tatgtctgct
    59821 tttcaggtct tattgcagcc atattacttg gaatgattta ctttgcactt gcatacgttg
    59881 gggcatcaac agctccagga aactttaaag atggtacaga tatattgacg tacaactcat
    59941 tacgattatt tggttcgttc ggtaacctcg tatttggaat gacggttatc cttgcatgcc
    60001 taacaacatg tataggactc gtcaatgctt gcgccacatt tactaagaaa cacgtaccta
    60061 agttttctta taaaatattc gcacttattt tctctatcat agggttctta tttacaacac
    60121 ttggtttaga aatgatttta aaaattgctg tcccattatt gactttaata tatcccgtgt
    60181 cgattgcact tgtactcata tcatttgcta acatgtttag cacattcaga ttcagttggg
    60241 cctatcgact cgcaactgtt attacattga ttatttcaat tttacaaata ctaaatagtt
    60301 tcaacttatt acacggtgtt attttgaaat cgtttatgat gttaccttta gcagatatcg
    60361 atttagcttg gcttgtacca ttcatgctct ttgctattat cggtttcata atcgatgtat
    60421 ttatacgccg tccgaaacaa gcgacaactt aataaatgct cactgcctag taatgattga
    60481 cccatcgtta ctaggctttt ttatatgaaa ttcatgactt ttcagtgata ccgtctgtct
    60541 attgattatt ttaaaataaa tgatttgtta aaaaatccta gctgttattc aaaaatatta
    60601 gtttttaaaa ttaattttgt gaaattattc acaaataagg caattcatag tatactctga
    60661 ttaacaaatg aaaagtgagg atgtcattgt gaaggagaaa catattaaat gggataagat
    60721 tttttatggg gacgactggg taaataacgt agtcgaaacg ataagaacaa aagatatatt
    60781 acaaagcgtt tatttaaaac gttatttatt acgcgcgatg atggcaggat ttattatcgg
    60841 gattattacg gtcttcgtat tatcagttaa agcaacacac gaaccagatt taccgccagg
    60901 cattgtgaat atggccagtg ccattacatt cagctttgcg ttagtactca ttttatttac
    60961 aaactccgaa ctactaacca gtaacttcat gtactttact gtaggcctgt attataaagt
    61021 aattaaacca actagagtat tgaaaatatt tttattatgc tttgcaggaa atattttagg
    61081 tgctgctatt ttatttagtt tcatgcgttt ttcaaatgta atgacgccag atatgttaaa
    61141 tcagttatca gcagttatag agcataaaac gttgtctact ggttttgtaa gtatattaat
    61201 gaaagcgatc ttcgccaatt tctttatcaa tatttcatta gttattgcca tgcaaattga
    61261 cgatgtactc gcaaaaatgt ttgtcatgat gttcggtgtt accatattcg cctttatggg
    61321 ttatgaacac gtcgtttaca acagttgctt atttatgggt ggtttaattt atcaagttga
    61381 tacattgcac ttcataccag ctatttcgaa tatcgctgcg gcttttatcg gtaactatat
    61441 tggaggcgga ctcattatag gtttattcta tgcttacttg aatgatcatc atcaatttta
    61501 taaaaataat taactatatt tgaacaatgc gttagtcgat gcctttaata tttagaagtg
    61561 cattgtctaa cgtatttttt taaagttcgt caatgatttt tatccgatgc tatgagcatt
    61621 atcaaataca aaatgctctc ttaaaagcag ttattgactg aaaatctact tctaagagag
    61681 cactttattt aattacttaa gaaatcttga aatttcaata tacgatgttt atgataagtc
    61741 gcttatttca tctttaggct tgttattagt aagtagttta ataccactga ttaaccataa
    61801 agcaaatgta attatgttac cacttattac agctccaata atcaacaata taccactcat
    61861 ttttttgttt ttagatgctt taaacatacc gattgcacct aaaataattg aaatgattcc
    61921 aaatatgaat agggataaga ataatacagt gaaaattgct gctgctgttt ctgcatcaac
    61981 tgggtcaacc tcaccattaa ctgttgttga cacatctgta gtcattacac ctgatgctgc
    62041 taaaaccatc aatagtaaac taaataccgt tgctaaaccc tgaacaacaa ttcctattat
    62101 tgtcattatt ttgatcgatt tttccattgt cttactcctt tattattatt atttacaaac
    62161 ttgcgtttgt gttcttatat ataaatgttt aaatttatat cgtgtttatt ttaaaaatta
    62221 ttgtcgctaa agctcattta ctttttgaaa ctggttaaaa gttttaaaga aagcctatgt
    62281 taatcttttt ccacttctac attttcatct tcataatctg ctttatggtc tttcatatac
    62341 ttaattgggt ctatttctct attaatctct gccttaccat cttttatttc atatgcgaaa
    62401 taatacattt tttcacgatc tttatataat tgaataccaa taacaatgac tttaccatct
    62461 ttataaacat gtgtattact gtcttcttgt ttaatgtctg ttttaatagt gttttgtact
    62521 ttcgaggcaa ttttattcat ttgattctgt tctttgtaca ctttatcaat atctttttga
    62581 tacttttca
//
LOCUS       DAMQYT010000002        45859 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMQYT010000002 DAMQYT010000000
VERSION     DAMQYT010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 45859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45859
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.002"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..1425)
                     /locus_tag="PGA33_000060"
     CDS             complement(<1..1425)
                     /locus_tag="PGA33_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6471902.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKF"
     gene            complement(1440..1754)
                     /locus_tag="PGA33_000061"
     CDS             complement(1440..1754)
                     /locus_tag="PGA33_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HDG6471903.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            complement(1732..2550)
                     /gene="recX"
                     /locus_tag="PGA33_000062"
     CDS             complement(1732..2550)
                     /gene="recX"
                     /locus_tag="PGA33_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDG6471904.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEIFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            complement(2811..3620)
                     /gene="sgtB"
                     /locus_tag="PGA33_000063"
     CDS             complement(2811..3620)
                     /gene="sgtB"
                     /locus_tag="PGA33_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HDG6471905.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            complement(3961..4476)
                     /locus_tag="PGA33_000064"
     CDS             complement(3961..4476)
                     /locus_tag="PGA33_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDG6471906.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            complement(4607..4768)
                     /locus_tag="PGA33_000065"
     CDS             complement(4607..4768)
                     /locus_tag="PGA33_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471907.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            5095..5196
                     /locus_tag="PGA33_000066"
     CDS             5095..5196
                     /locus_tag="PGA33_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471908.1"
                     /translation="MRFFAFFIFLIFSVLQQNGIKNGIICSYFSFTY"
     gene            complement(5522..5707)
                     /locus_tag="PGA33_000067"
     CDS             complement(5522..5707)
                     /locus_tag="PGA33_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471909.1"
                     /translation="MSDYKLKIIELIKSDITGYQIHKQTGVAQYVISQLRQGKREVDN
                     LTLNTTEKLYSYARQVL"
     gene            complement(5709..5819)
                     /locus_tag="PGA33_000068"
     CDS             complement(5709..5819)
                     /locus_tag="PGA33_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471910.1"
                     /translation="MTEQMYLILFLLSLPLLLFIGRKTHFYCLDKKNGRR"
     gene            complement(5890..6042)
                     /locus_tag="PGA33_000069"
     CDS             complement(5890..6042)
                     /locus_tag="PGA33_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471911.1"
                     /translation="MCKEVKSLMLDIIKTLLEHQVLAVLIIPEVLKQLREWHLGYLDR
                     KPNNKD"
     gene            complement(6285..7730)
                     /locus_tag="PGA33_000070"
     CDS             complement(6285..7730)
                     /locus_tag="PGA33_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDG6471912.1"
                     /translation="MQAKLTKKEFIEWLKTSEGKQYNADGWYGFQCFDYANAGWQVLF
                     GYNLKGVGAKDIPSANDFNGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEA
                     TLDYIIVYEQNWLGGGWTDGVQQPGSGWEKVTRRQHAYDFPMWFIRPNFKSETAPRSV
                     QSPTQASKKETAKPQPKAVELKIIKDVVKGYDLPKRGSNPKFIVIHNDAGSKGATAEA
                     YRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQIGNKYGYGIEVCQSM
                     GADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDP
                     VTRGLLPEDKRLQLKDYFIKQIRAYMDGKIPVATVSNESSASSNTVKPVASAWKRNKY
                     GTYYMEESARFTNGNQPITVRKVGPFLSCPVGYQFQPGGYCDYTEVMLQDGHVWVGYT
                     WEGQRYYLPIRTWNGSAPPNQILGDLWGEIS"
     gene            complement(7711..8148)
                     /locus_tag="PGA33_000071"
     CDS             complement(7711..8148)
                     /locus_tag="PGA33_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDG6471913.1"
                     /translation="MDINWKLRFKNKAVLTSLVGALLLFIKQVTDLFGLDLSTQLNQA
                     SAIIGAILTLLTGIGVITDPTSKGVSDSSIAQTYQAPRDSDKEEQQVTWKSSQDSSLT
                     PELSTKAPKEYDTSQPFTDASNDVGFDVNEYHHGGGDNASKTN"
     gene            complement(8204..8599)
                     /locus_tag="PGA33_000072"
     CDS             complement(8204..8599)
                     /locus_tag="PGA33_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471914.1"
                     /translation="MEGNFKNVKKLIYEGEEYTKVYAGNIQVWKKPSYFVIKPLPKNK
                     YPDSIEESTAKWTINGVEPNKSYQVTIENVRSGIMRISQTNLGSSELGISGVNSGVAS
                     KNINFSNPSGTLYVTISDVYSGSPTLTIE"
     gene            complement(8605..9777)
                     /locus_tag="PGA33_000073"
     CDS             complement(8605..9777)
                     /locus_tag="PGA33_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDG6471915.1"
                     /translation="MYKIKDVETRIKNDGVDLGDIGCRFYTEDENTASIRIGINDKQG
                     RIDLKAHGLTPRLHLFMEDGSIFKNEPLIIDDVVKGFITYKIPKKVIKHAGYVRCKLF
                     LEKEEEKIHVANFSFNIVDSGIESAVAKEIDVKLVDDAITRILKDNATDLLNKDFKEK
                     IDKDVISYIEKNESRFKGAKGDKGEPGQPGAKGEAGKKGEQGAPGKNGTVVSINPDTK
                     MWQIDGKDTDIKAEPELLDKINIANVEGLEDKLQEVKKIQDTTLNDSKTYTDSKIAEL
                     VDSAPESMNTLRELAEAIQNNSISESVLQQIGSKVSAEDFEEFKQTLNDLYAPKNHNH
                     DERYVLSSQAFTKQQADNLYQLKSASQPTVKIWTGTENEYNYIYQKDPNTLYLIKG"
     gene            complement(9790..11688)
                     /locus_tag="PGA33_000074"
     CDS             complement(9790..11688)
                     /locus_tag="PGA33_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDG6471916.1"
                     /translation="MGLPNPKTRKPTASEVVEWALYIAKNKIAIDVPGSGMGAQCWDL
                     PNYLLDKYWGFRTWGNADAMAQKSNYRGRDFKIIRNTKDFVPQPGDWGVWTGGWAGHV
                     NIVVGPCTKDYWYGVDQNWYTNNATGSPPYKIKHSYHDGPGGGVKYFVRPPYHPEKST
                     PAPKPEDDSDDNEKNNKKVPIWKDVTTIKYTISSQEVNYPEYIYHFIVEGNRRLEKPK
                     GIMIRNAQTMSSVENLYNSRKKYKQDVEYPHFYVDRHNIWAPRRAVFEVPNEPDYIVI
                     DVCEDYSASKNEFIFNEIHAMVVAVDMMAKYEIPLSIENLKVDDSIWRSMLEHVNWNM
                     IDNGVAPKDKYEALEKALLNIFKNREKLLNSITKPTVTKSRIKVMVDNKNADIANVRD
                     SSPTANNGSASKQPQIITETSPYTFKQALDKQMARGNPKKSNAWGWANATRAQTGSAM
                     NVKRIWESNTQCYQMLNLGKYQGVSVSSLNKILKGKGTLNNQGKAFAEACKKHNINEI
                     YLIAHAFLESGYGTSNFANGKDGVYNYFGIGAYDNNPNYAMTFARNKGWTSPAKAIMG
                     GASFVRKDYINKGQNTLYRIRWNPKNPATHQYATAIEWCQHQASTIAKLYKKIGLKGI
                     YFIRDKYK"
     gene            complement(11825..12124)
                     /locus_tag="PGA33_000075"
     CDS             complement(11825..12124)
                     /locus_tag="PGA33_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HDG6471917.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRAQEKIYDK
                     LDRNFEELKRDKVEDEKNKEKNAKNIRDIKMWILGLVGTIFSTIVIALLRTIFGI"
     gene            complement(12165..12338)
                     /locus_tag="PGA33_000076"
     CDS             complement(12165..12338)
                     /locus_tag="PGA33_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDG6471918.1"
                     /translation="MKMIYPTFKDIKTFYVWGCYKNEQIKWYVDMGVIDKEEYALITG
                     EKYPETKDEKSQV"
     gene            complement(12342..12719)
                     /locus_tag="PGA33_000077"
     CDS             complement(12342..12719)
                     /locus_tag="PGA33_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HDG6471919.1"
                     /translation="MKITVNDKNEVIGYVNTGGLRNSLDVDDNNVPIKFKEEFEPRKF
                     VFTNGEIKYNSNFEKEDVPNASKQQSESDLSDEELRGMVASMQMQMTQVNMLTMQLTQ
                     QNAMLTQQLTELKTNKTNTEGDV"
     gene            complement(12719..14542)
                     /locus_tag="PGA33_000078"
     CDS             complement(12719..14542)
                     /locus_tag="PGA33_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDG6471920.1"
                     /translation="MVVDNFSKDDNLIELKTTSQYNPVIDTNISFYESDRGTGVLNFA
                     VTKNNRPLSISSEHVKTSIVLKTDDYNVDRGAYISDELTVVDAINGRLQYVIPNEFLK
                     HSGKVHAQAFFTQHGSNNVVVERQFSFNIENDLVSGFDGITKLVYIKSIQDTIEAVGK
                     DFNQLKQNMADTQTLIAKVNDSATKGIQQIEIKQNEAIQAITATQTSATQAVTAEFNK
                     IVEKEQTIFARVNEVEQQINGADLVKGNSTVNWQKSKITDDYGKAIESSEQSIDSVLS
                     TVNTSRIIHITNATDAPEKTDIGTLEKPGQDGVDDGSSFDESTYTSSKSGVLVVYVVD
                     NNTARATWYPDDSNDEYTKYKIYGTWYPFYKKNDGNLTKQFVEEISNNALNQAKQYVD
                     GKFQSTSWQQHKLTEHNGQSIQKNLYNAKGNLEALGAGNYYVTSVPDLPGIVESYEGY
                     LSVFVKDDANKLFNFTPSNSKKVYTRSITNGRLDSQWAAPNEHKTAVLFDGAANGVGT
                     RINLTEAYTNYAILFISGTYPGGVIEAFSLTSIPNAIQLSKTNVVDSDGNGGGSYECL
                     ITKESGTTLKIDNDVYLDLGSKTGSGANANRVTINKIVGWK"
     gene            complement(14542..16452)
                     /locus_tag="PGA33_000079"
     CDS             complement(14542..16452)
                     /locus_tag="PGA33_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471921.1"
                     /translation="MDNKLITDLSRVFDYRYVDENEYNFKLISDMLTDFNFSLEYHRN
                     KEVFAHNGEQIKYEHLNVTSSVSDFLTYLNGRFSNMVLGHNGDGINEVKDARVDNTGY
                     DHKTLQDRLYHDYSTLDTFTKKVEKAVDEHYKEYQATEYRFEPKEQEPEFITDLSPYT
                     NAVMQSFWIDPRTKIIYMTQARPGNHYMLSRLKPNGQFIDRLLVKNGGHGTHNAYRYI
                     DGELWIYSAVLDSNKNNKFVRFQYRTGEITYGNEMQDVMPNIFNDRYTSAIYNPIENL
                     MIFRREYKASERQAKNSLNFIEVRSADDIDKGIDKVLYQMDIPMEYTSDTQPMQGITY
                     DAGILYWYTGDSNTANPNYLQGFDVKTKELLFKRRIDIGGVNNNFKGDFQEAEGLDMY
                     YDLETGRKALLIGVTIGPGNNRHHSIYSIGQRGVNQFLKNIAPQVSMTDSGGRVKPLP
                     IQNPAYLSDITEVGHYYIYTQDTQNALDFPLPKAFRDAGWFFDVLPGHYNGALRQVLT
                     RNSTGRNMLKFERVIDIFNKKNNGSWNFNPQSAGYWEHIPKSITKLSDLKIVGLDFYI
                     TTEESKRFSDFPKDYKGIAGWVLEVKSNTPGNTTQVLRRNNFASAHQFFVRNFGTGGN
                     SGWSIIEGKEVE"
     gene            complement(16467..18368)
                     /locus_tag="PGA33_000080"
     CDS             complement(16467..18368)
                     /locus_tag="PGA33_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDG6471922.1"
                     /translation="MTITVKPPKGNGAPIPVETTLVKKVNADGVLTFDILENKYTYEV
                     INAIGKRWIVSHVEGENDKKEYVITVIDRKSEGDRQLVECTAREIPIDKLMIDRIYVN
                     VTGSFTVERYFNIVFQGTGMLFEVEGKVKSSKFENGGEGDTRLEMFKKGLEHFGLEYK
                     ITYDKKKDRYKFVLTPFANQKASYFISDEVNANAIKLEEDASDFATFIRGYGNYSGEE
                     TFEHAGLVMEARSALAEIYGDIHAEPFKDGKVTDQETMDKELQSRLKKSLKQSLSLDF
                     LVLRESYPEADPQPGDIVQIKSTKLGLNDLVRIVQVKTIRGINNVIVKQDVTLGEFNR
                     EQRYMKKVNTAANYVSGLNDVNLSNPSKAAENLKSKVASIAKSTLDLMSRTDLIEDKQ
                     QKVSSKTVTTSDGTIVHDFIDKSNIKDVKTIGTIGDSVARGSHAKTNFTEMLGKKLKA
                     KTTNLARGGATMATVPIGKEAVENSIYRQAEQIRGDLIILQGTDDDWLHGYWAGVPIG
                     TDKTDTKTFYGAFCSAIEVIRKNNPASKILVMTATRQCPMSGTTIRRKDTDKNKLGLT
                     LEDYVNAQILACSELDVPVYDAYHTDYFKPYNPAFRKSSMPDGLHPNERGHEVIMYEL
                     IKNYYQFYG"
     gene            complement(18377..19324)
                     /locus_tag="PGA33_000081"
     CDS             complement(18377..19324)
                     /locus_tag="PGA33_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDG6471923.1"
                     /translation="MDIELTKKDGTVIKLSEYGFIVNDIVIDSMQINTKYQDKENMNG
                     RILMGSNYISRDIVVPCFCKVKNRSDIAYMRDMLYSLTTDIEPMYLREIRRKEELNYR
                     FTQPTSDDYVKLDKNNFPDYEYSRHDQQIYVNGKQYKVIFSGVINPKQKGNKVAFELK
                     FETTELPYGESIGTSLELEENKKVGLWSFDFNIDWHAGGDKRQYTFENVSKDTVYYHG
                     SAPNDQFNMYKKITIILGEDTESFVWNLTHAEIMKIEGIKLKAGDKIVYDSFRVYKNG
                     VEISTETNIAQPKFKYGANKFEFNQTVQKVQFDLKFYYK"
     gene            complement(19337..22804)
                     /locus_tag="PGA33_000082"
     CDS             complement(19337..22804)
                     /locus_tag="PGA33_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471924.1"
                     /translation="MTEYKIKATIEASVAKFKRQIDSAVKSVQRFKRVADQTKDVELN
                     ADDKKLQKTIKVAKKSLDAFSNKKVKAKLDASIQDLQQKVLESNFELDKLNSKEVTPE
                     IKLQKQKLTKDIAEAEAKLSELEKKRVNIDINADNSKFNRVLKVSKASLEALNRSKAK
                     AIIDVDNGVANSKIKRTKEELKSIPNKTRSRLDVDTGLSIPTIYAFKKSLDALPNKKT
                     TKVDVDTNGLKKAYAYIIKANDNFQRQMGNLANMFRVFGTVGSNMVGGLLTSSFSILI
                     PVIASVVPVVFALLNAIKVLTGGVLALGGAVAIAGAGFVAFGAMAISAIKMLNDGTLQ
                     ASSATNEYKKALDGVKSAWTDIIKQNQSAIFTTLANGLNTVKTAMQSLQPFFSGISRG
                     MEEASQSVLKWAENSSVASRFFNMMNTTGVSVFNKLLSAAGDFGDGLVNVFTQLAPLF
                     QWSADWLDRLGQSFSNWANSAAGENSITRFIEYTKTNLPIIGNIFKNVFVGINNLMNA
                     FSGSSTGIFQSLEQMTAKFREWSEQVGQSQGFKDFVSYIQTNGPLIMQLIGNIARGLV
                     AFATAMAPIASAVLRVAVAITGWIANLFEAHPATAQLVGVIITLVGAFRFLIPIILAV
                     SNFMGGGLIGRIIALVSKFGLLRAGLTILKGAFMLLKGPLKIISVIFQLLFGKIGLIR
                     NAITGLVTVFGILGGPITIVIGVIAALIAIFVLLWNKNEGFRNFIINAWNAIKTFMVT
                     VWNVLKTVASVVWNAILKAITTAVSNVYNFIMIIWNQIVAYLQGLWNGIIAIATTVWN
                     LLVTIITTVFTTIMTIVMTIWTAIWTFLSTIWNTIITIATTIWNLLVTVITTVFTTIM
                     TIAMTIWNAIWTFLQTLWNTIVTVATKVWNAITTTISTALQAAWSFISNIWNTIWSFL
                     SGILTTIWNKVVSIFTQVVSTISDKMSQAWNFIVTKGMQWVSTITSTLINFVNRVVQG
                     FVNVVNKVSQGMTNAVNKVKSFVDDFVSAGADMIRGLMRGIGNMARDLAEKAASVAKG
                     ALNAAKRALGIHSPSREFMDVGMYSMLGFVKGIDNHSSKVIRNVSNVADKVVDAFQPT
                     LNAPDISSITGNLSNLGGNINAQVQHTHSIETSPNMKTVKIEFDVNNDALTSIVNGRN
                     AKRNSEYYL"
     gene            complement(22821..23165)
                     /locus_tag="PGA33_000083"
     CDS             complement(22821..23165)
                     /locus_tag="PGA33_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471925.1"
                     /translation="MSTLGITDLNVIEQMTLTEYNYRMYAKEYEMLTQEFERYKLAFA
                     IRDAAATKNVGTENKPKEEYVFNNANDVLPYEENIQRLNEGKDIRFSSERDEYEPQNN
                     EFFKVIAEFNKQ"
     gene            complement(23195..23560)
                     /locus_tag="PGA33_000084"
     CDS             complement(23195..23560)
                     /locus_tag="PGA33_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HDG6471926.1"
                     /translation="MNVEINGKSLELSFGFKFLREIDNRLGLKVEQASIGQGVSMLPV
                     GLESGNPVVIGEVLIAATSHLKKQAITINNIDEALDEIAENIGLEEFGSDILTELGKR
                     PMTRNLVEVVETEEKPAEA"
     gene            complement(23622..24203)
                     /locus_tag="PGA33_000085"
     CDS             complement(23622..24203)
                     /locus_tag="PGA33_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HDG6471927.1"
                     /translation="MANMKNSNDRIILFRKAGEKVDATKMLFLTEYGLSHEADTDTED
                     TMDGSYNTGGSVESTMSGTAKMFYGDDFADEIEDAVVDRVLYEAWEVESRIPGKNGDA
                     TKFKAKYFQGFHNKFELKAEANGIDEYEYEYGVNGRFQRGFATLPEAVTKKLKATGYR
                     FHDTTKADALTGEDLTAIPQPKVDSSTVTPGEV"
     gene            complement(24222..24605)
                     /locus_tag="PGA33_000086"
     CDS             complement(24222..24605)
                     /locus_tag="PGA33_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471928.1"
                     /translation="MTPNLQLYNKAYETLQGYGFPVISRKEMQQEIPYPFFVIKMPES
                     NRSKYTFDSYSGDTNLVIDIWSVSDDLGHHDGLVKRCIDDLTPSVKTNDYDFEEDDTN
                     IAQLVDDTTNQELLHTSITISYKTF"
     gene            complement(24617..24964)
                     /locus_tag="PGA33_000087"
     CDS             complement(24617..24964)
                     /locus_tag="PGA33_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDG6471929.1"
                     /translation="MNIDGLDALLNQFHDMKTNIDDDVDDILQENAKEYVVRAKLKAR
                     EVMNKGYWTGNLSRNIRYKKTGDLQYTITSHAAYSGFLEFGTRYMEAEPFMWPVYEVI
                     RKSTVEELKALFE"
     gene            complement(24964..25266)
                     /locus_tag="PGA33_000088"
     CDS             complement(24964..25266)
                     /locus_tag="PGA33_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471930.1"
                     /translation="MRYEDRVIFQLEQVATYNPKTSKKENTLITYDAIPCNINPISRA
                     RKQLEFGDVKNDISVLRIKESISYPVSHVLVNGIRYKIVDTRIYRHETSYYIEEVN"
     gene            complement(25263..25595)
                     /locus_tag="PGA33_000089"
     CDS             complement(25263..25595)
                     /locus_tag="PGA33_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HDG6471931.1"
                     /translation="MTTLADVKKRIGLKDEKQDEQLEEIIKSCESQLLSMLPIEVEQI
                     PERFSYMIKEVAVKRYNRIGAEGMTSEAVDGRSNAYELNDFKEYEAIIDNYFNARTRT
                     KKGRAVFF"
     gene            complement(25604..25891)
                     /locus_tag="PGA33_000090"
     CDS             complement(25604..25891)
                     /locus_tag="PGA33_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471932.1"
                     /translation="MPEIIGIVKVDFTDLEDNRHVYMKGHVYPRKGYNPTDERIKALA
                     SVENKRNKQMIYIVNDKLTKKELVEIASVAGLQVDEKQTKAEIINAFESLE"
     gene            complement(25913..26887)
                     /locus_tag="PGA33_000091"
     CDS             complement(25913..26887)
                     /locus_tag="PGA33_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDG6471933.1"
                     /translation="MEQTQKLKLNLQHFASNNVKPQVFNPDNVMMHEKKDGTLLNDFT
                     TPILQEVMENSKIMQLGKYEPMEGTEKKFTFWADKPGAYWVGEGQKIETSKATWVNAT
                     MRAFKLGVILPVTKEFLNYTYSQFFEEMKPMIAEAFYKKFDEAGILNQGNNPFGKSIA
                     QSIEKTNKVIKGDFTQDNIIDLEALLEDDELEANAFISKTQNRSLLRKIVDPETKERI
                     YDRNSDSLDGLPVVNLKSSNLKRGELITGDFDKLIYGIPQLIEYKIDETAQLSTVKNE
                     DGTPVNLFEQDMVALRATMHVALHIADDKAFAKLVPADKRTDSVPGEV"
     gene            complement(26901..27521)
                     /locus_tag="PGA33_000092"
     CDS             complement(26901..27521)
                     /locus_tag="PGA33_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDG6471934.1"
                     /translation="MEENKLKFNLQFFADQSDDPDEPGGDGKKGNPDKKENDEGTEIT
                     FTPEQQKKVDEILERRVAHEKKKADEYAKEKAAEAAKEAAKLAKMNKDQKDEYEREQM
                     EKELEQLRSEKQLNEMRSEARKMLSEAEVDSSDEVVNLVVTDTAEQTKLNVEAFSNAV
                     KKAVNEAVKVNARQSPLTGGDSFNHSTKNKPQNLAEIARQKRIIKN"
     gene            complement(27630..27800)
                     /locus_tag="PGA33_000093"
     CDS             complement(27630..27800)
                     /locus_tag="PGA33_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471935.1"
                     /translation="MSNTDKYLRDIARELKGIRKELQKRNETVIIDANLDSLRSAVLA
                     DKEKSKYNEPLF"
     gene            complement(27873..28868)
                     /locus_tag="PGA33_000094"
     CDS             complement(27873..28868)
                     /locus_tag="PGA33_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDG6471936.1"
                     /translation="MPNKNTQEYWEERGRKAIENELKRDKTKAEEIERILNMMIKRIE
                     KEINAFIVKYGDFAGVTLQEAQKIIDEFDVKAFQEEAKRLVENKEFSDRANEELKKYN
                     TKMYVSREQMLKIQIEFLIAYATAQTELSMREYFESTAYRVFSDQAGILGEGVQVAKE
                     VIDTIVDTQFHGVVWSERLWTNTEAMKQEVEEIIANVVIRGRHPNEYVKDMRKHLNKF
                     EGTARQKTAAIKSLLYTESARVHAQSSIDSMKEISPEGYYMYIAKIDNRTTKVCKGLN
                     GEIFKVKDAKIGVNFYPMHINCRSDCALLPKSMWPKKPSKKRKTKYFGGKVKSGD"
     gene            complement(28875..30410)
                     /locus_tag="PGA33_000095"
     CDS             complement(28875..30410)
                     /locus_tag="PGA33_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDG6471937.1"
                     /translation="MLKVNEFETDTDLRGNINYLFNDEANVVYTYDGTESDLLQNVNE
                     VSKYIEHHMDYQRPRLKVLSDYYEGKTKNLVELTRRKEEYMADNRVAHDYASYISDFI
                     NGYFLGNPIQYQDDDKDVLEAIEAFNDLNDVESHNRSLGLDLSIYGKAYELMIRNQDD
                     ETRLYKSDAMSTFVIYDNTIERNSIAGVRYLRTKPIDKTDEDGVFTVDLFTSHGVYRY
                     LTSRTNGLKLTPRENGFESHSFERMPITEFSNNERRKGDYEKVITLIDLYDNAESDTA
                     NYMSDLNDAMLLIKGNLNLDPVEVRKQKEANVLFLEPTVYADSEGRETEGSVDGGYIY
                     KQYDVQGTEAYKDRLNSDIHMFTNTPNMKDDNFSGTQSGEAMKYKLFGLEQRTKTKEG
                     LFTKGLRRRAKLLETILKNTWSIDANKDFNTVRYVYNRNLPKSLIEELKAYIDSGGKI
                     SQTTLMSLFSFFQDPELEVKKIEEDEKESIKKAQKGIYKDPRDINDDEQDDDTKDTVD
                     KKE"
     gene            complement(30421..31698)
                     /locus_tag="PGA33_000096"
     CDS             complement(30421..31698)
                     /locus_tag="PGA33_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDG6471938.1"
                     /translation="MTKVKLNFNKPSNVFNRNIFEILTNYDNFTEVHYGGGSSGKSHG
                     VIQKVVLKALQDWKYPRRILWLRKVQSTIKDSLFEDVKDCLINFGIWDMCLWNKTDNK
                     VELPNGAVFLFKGLDNPEKIKSIKGISDIVMEEASEFTLNDYTQLTLRLRERKHVNKQ
                     IFLMFNPVSKLNWVYKYFFEHGEPMENVMIRQSSYRDNKFLDEMTRQNLELLANRNPA
                     YYKIYALGEFATLDKLVFPKYEKRLINKDELRHLPSYFGLDFGYVNDPSAFIHSKIDV
                     KKKKLYIIEEYVKQGMLNDEIANVIKQLGYAKEEITADSAEQKSIAELRNLGLKRILP
                     TKKGKGSVVQGLQFLMQFEIIVDERCFKTIEEFDNYTWQKDKDTGEYTNEPVDTYNHC
                     IDSLRYSVERFYRPVRKRTNLSSKVDTIKSLGL"
     gene            complement(31685..32125)
                     /locus_tag="PGA33_000097"
     CDS             complement(31685..32125)
                     /locus_tag="PGA33_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDG6471939.1"
                     /translation="MNEKQKRFADEYIMNGCNGKKAAITAGYSKKTAESLASRLLRNV
                     NVSEYIKERLEQIQEERLMSITEALALSASIARGEPQEAYSKKYDHLNDEVEKEVTYT
                     ITPTFEERQRSIDHILKVHGAYIDKKEITQKNIEINIGEYDDES"
     gene            complement(32312..32734)
                     /locus_tag="PGA33_000098"
     CDS             complement(32312..32734)
                     /locus_tag="PGA33_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RinA family phage transcriptional activator"
                     /protein_id="HDG6471940.1"
                     /translation="MTKKKYGLKLSTVRKLEDELCDYPNYHKQLEDLRSEIMTPWIPT
                     DTNIGGEFVPSNTSKTEMAVTNYLCSIRRGKILEFKSAIERIINTSSRKEREFIQEYY
                     FNKKELVKVCVDIHISDRTAHRIKRKIISRLAEELGED"
     gene            complement(32758..32904)
                     /locus_tag="PGA33_000099"
     CDS             complement(32758..32904)
                     /locus_tag="PGA33_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471941.1"
                     /translation="MMWLVIAIILLVILLFGVMLQAEQLKGDVKVKEREIEILRSRLR
                     HFED"
     gene            complement(32905..33078)
                     /locus_tag="PGA33_000100"
     CDS             complement(32905..33078)
                     /locus_tag="PGA33_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator RinB"
                     /protein_id="HDG6471942.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEKVYIMTTANDDVEAPSDFAKL
                     SDQSDLMRAEVSE"
     gene            complement(33078..33464)
                     /locus_tag="PGA33_000101"
     CDS             complement(33078..33464)
                     /locus_tag="PGA33_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471943.1"
                     /translation="MMIKKLKNMDWFDIFIVGILRLFGVIALMLVVISPIYTVASYQN
                     KEVHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKV
                     EVKTIGYRIHFLNLYPVLYEVKKVDK"
     gene            complement(33454..33690)
                     /locus_tag="PGA33_000102"
     CDS             complement(33454..33690)
                     /locus_tag="PGA33_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471944.1"
                     /translation="MFNTPKMKLPEKHTEVFKTYKNGTPEEKAEIEGCFIKTVKDEDS
                     EFYSPMLASLNEQQLKSMLRQVLFLIDTGDDNDD"
     gene            complement(33715..33951)
                     /locus_tag="PGA33_000103"
     CDS             complement(33715..33951)
                     /locus_tag="PGA33_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HDG6471945.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITRAKSNQRFTVVEAESKEEAKEKY
                     ESQNTPIVYYTNNSKVTLFERPSEEVLGSLFEKK"
     gene            complement(33948..34193)
                     /locus_tag="PGA33_000104"
     CDS             complement(33948..34193)
                     /locus_tag="PGA33_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471946.1"
                     /translation="MSYIITLVLILVFIVIFNNLLNRYMVLYKELDLFTCRIGMLLVL
                     IVIVDFAKQQNMLATLSVLLILLFVEKLRIIQRSDKK"
     gene            complement(34230..34739)
                     /locus_tag="PGA33_000105"
     CDS             complement(34230..34739)
                     /locus_tag="PGA33_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HDG6471947.1"
                     /translation="MTNILQVKLLSENARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDAIASNGY
                     ITPGVFDIKGEIDLSDAIRQYGTYQINEGDKLAQLVIVPIWTPELKQVEEFESVSERG
                     EKGFGSSGV"
     gene            complement(34732..34974)
                     /locus_tag="PGA33_000106"
     CDS             complement(34732..34974)
                     /locus_tag="PGA33_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471948.1"
                     /translation="MKINESLKKLKEKGYKENEDKAIFNLADGTLEIYIDHDEKTIIT
                     EFHDLKVFVSEDLKDKSMESVMYELAGIDEEDKEND"
     gene            complement(34964..35200)
                     /locus_tag="PGA33_000107"
     CDS             complement(34964..35200)
                     /locus_tag="PGA33_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDG6471949.1"
                     /translation="MDNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEIRE
                     AINAQWVEYPEDWASEVLREVRELEYEEEQENEN"
     gene            complement(35193..35366)
                     /locus_tag="PGA33_000108"
     CDS             complement(35193..35366)
                     /locus_tag="PGA33_000108"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471950.1"
                     /translation="MEMMNAEKHMQMMQMLQNCVIDKYVSHDEYEELIAIDKHGNKMF
                     IKFYPNTEDDTNG"
     gene            complement(35372..35620)
                     /locus_tag="PGA33_000109"
     CDS             complement(35372..35620)
                     /locus_tag="PGA33_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HDG6471951.1"
                     /translation="MTMIDNARKEYLNQFFGSKRYLYQDNERVAHIHVVNGTYYFHGH
                     IVPGWQGVKKIFDTAEELETYIKQHGLEYEEQKQLTLF"
     gene            complement(35620..35874)
                     /locus_tag="PGA33_000110"
     CDS             complement(35620..35874)
                     /locus_tag="PGA33_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HDG6471952.1"
                     /translation="MSVISNRKVDMNKTQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVQRAFDLWD"
     gene            complement(35874..36236)
                     /locus_tag="PGA33_000111"
     CDS             complement(35874..36236)
                     /locus_tag="PGA33_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471953.1"
                     /translation="MGSVVIINNKPYKFNNFEKELMAKRGINAGIVSKRVRGCWEFSE
                     ALDAPYGMHLKEYREMKQMEKIKQARLERELERERKKEAELRKKKPHLFNVPRKHSRD
                     PYWFDVTYNQMFKKWSEA"
     gene            complement(36237..36422)
                     /locus_tag="PGA33_000112"
     CDS             complement(36237..36422)
                     /locus_tag="PGA33_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HDG6471954.1"
                     /translation="MQQQAYINATIDIRIPTEVEYQHFDDVDKEKETLADYLYNNPDE
                     ILEYDNLKIRNVNVEVE"
     gene            complement(36427..36831)
                     /locus_tag="PGA33_000113"
     CDS             complement(36427..36831)
                     /locus_tag="PGA33_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDG6471955.1"
                     /translation="MSKYNAKKVEYKGIVFDSKVECEYYQYLESNMNGTNYDRIELQP
                     KFELLPKLDKQRKIEYIADFALYLDDKLIEVIDIKGMPTEVAKLKAKMFRHKYRNIKL
                     NWICKAPKYTGKTWITYEELIKARRERKREMK"
     gene            complement(36841..37062)
                     /locus_tag="PGA33_000114"
     CDS             complement(36841..37062)
                     /locus_tag="PGA33_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3269 family protein"
                     /protein_id="HDG6471956.1"
                     /translation="MPKEKYYLYREDGTEDIKVIKHEDNENEVYSLTGAHFSDEKKIM
                     TDSDLKRFKGAHGLLYEQELGLQATIFDI"
     gene            complement(37075..37233)
                     /locus_tag="PGA33_000115"
     CDS             complement(37075..37233)
                     /locus_tag="PGA33_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471957.1"
                     /translation="MVTKEFLKTKLECSDIYAQKLIDEAQGDENRLYDLFIQKLAERH
                     TRPAVVEY"
     gene            complement(37227..38000)
                     /locus_tag="PGA33_000116"
     CDS             complement(37227..38000)
                     /locus_tag="PGA33_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDG6471958.1"
                     /translation="MKPLFNEKINESLKKYQPIEVILRQNCDKCGHQYDLYKFENGYE
                     YKDGCECEIQRLAYEEYKRNKQKKLDYIFNQSNVNPSLRDATVNNYKPQNEKQVKAKQ
                     TAIEYVQGFSTKEPKSLILQGSYGTGKSHLAYAIAKAVKSKGHTVAFMHIPMLMDRIK
                     ATYNKNAVETTDELVRLLSDIDLLVLDDMGVENTEHTLNKLFSIVDNRVGKNNIFTTN
                     FSDKELNQNMNWQRINSRMKHNARKVRVIGDDFRERDAW"
     gene            complement(38010..38807)
                     /locus_tag="PGA33_000117"
     CDS             complement(38010..38807)
                     /locus_tag="PGA33_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDG6471959.1"
                     /translation="MGEVSWIKLKVGMFDDSKIKYIEALPERDTIITIWVKLLTLSGK
                     YNEQGYIMLSENLPYNEEMLANEFSRPINSIRLAIQTFETLGMIEKVNGVIKVTNWEK
                     HQNIEGLEKIRAQNRLRKQKQRENNRKLLNGHVTSRDSHATEEDKELDKELERDKEID
                     KNLSSNNSATDVTHEQFEEWWKLYNKKKDKKMSFTKFKSCVKKHTFEQIMQGTREYLK
                     TITDKQYQKYPKTFLTNESYMNDYSEEIKETGIDQLERMKYDESYWD"
     gene            complement(38779..39558)
                     /locus_tag="PGA33_000118"
     CDS             complement(38779..39558)
                     /locus_tag="PGA33_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDG6471960.1"
                     /translation="MVKSIFLQDGEEILVDDEDYERVNQHTWHKAFKDNYRMIVNSDK
                     KHLPDFILKKSFQKIKNNDFTRKNLTTEGNKTRWSKAKCNNSSKYKGVSWDKKNNNWY
                     ACIAVDKKTKNLGHFVNEDEAAKAYNNAVNEYWGGVGYLNIIGEDNRLKKRNYKTNIK
                     QLKRGTDKNNLRGINKIKHRYYSKIFYSGNYIALGGYDDLNKARLVYNKCSSYLHGSD
                     AILNDVPMTDELKEFISNWEVPDKIKALKGEDNGRSIVDKT"
     gene            complement(39558..40223)
                     /locus_tag="PGA33_000119"
     CDS             complement(39558..40223)
                     /locus_tag="PGA33_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471961.1"
                     /translation="MQYITRYQKDNDGTYSVVATGVELEQSHIDLLENGYPLKAEVEV
                     PDNKKLSIEQRKKIFAMCRDIELHWGEPVESTRKLLQTELEIEKGYEEISLRDCSMKV
                     ARELIELIIAFMFHHQIPMSVETSKLLSEDKALLYWATINRNCVICGKPHADLAHYEA
                     VGRGMNRNKMNHYDKHVLALCREHHNEQHAIGVKSFDDKYHLHDSWIKVDERLNKMLK
                     GGE"
     gene            complement(40236..40790)
                     /locus_tag="PGA33_000120"
     CDS             complement(40236..40790)
                     /locus_tag="PGA33_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDG6471962.1"
                     /translation="MKITGRTQYIQETNQEAFMKGGDFLGAGEFTVKVANVEFNDREN
                     RYFTIVFENNEGKQYKHNQFVPPFQQDYQEKQYIELLSRLGIKLNLPDLTFDTDQLIN
                     KIGTIVLKNKFNEEQGKYFVRLSYVKVWNKDDEVVNKPEPKTDEMKQKEQQANGKQTP
                     MSQQSNPFANANGPIEINDDDLPF"
     gene            complement(40820..41599)
                     /locus_tag="PGA33_000121"
     CDS             complement(40820..41599)
                     /locus_tag="PGA33_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDG6471963.1"
                     /translation="MTEQTNQDVDILTQLGVKDISKQNANKFYKFAIYGKFGTGKTTF
                     LTKDNNALVLDINEDGTTVTEDGAVVQIKNYKHFSAVIKMLPKIIEQLRENGKQIDVV
                     VIETIQKLRDITMDDIMDGKSKKPTFNDWGECATRIVSIYRYISKLQEHYQFHLAISG
                     HEGINKDKDDEGSTINPTITIEAQDQIKKAVISQSDVLARMTIEEHEQDGEKTYQYVL
                     NAEPSNLFETKIRHSSNIKINNKRFINPSINDVVQAIRNGN"
     gene            complement(41592..41813)
                     /locus_tag="PGA33_000122"
     CDS             complement(41592..41813)
                     /locus_tag="PGA33_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2483 family protein"
                     /protein_id="HDG6471964.1"
                     /translation="MKQTVTYIIRHRDMPIYITNKPTDNNSDISYSTNRNRAREFNGM
                     EEASINMDYHKAIKKTVTETIEYEEVEHD"
     gene            complement(41823..42083)
                     /locus_tag="PGA33_000123"
     CDS             complement(41823..42083)
                     /locus_tag="PGA33_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDG6471965.1"
                     /translation="MYYEIGEIIRKNIHVNGFNFNLFILKGHMGISIQVKDMNNVPIK
                     HAYVVDENDLDMASDLFNQAIDEWIEENTDEQDRLINLVMKW"
     gene            complement(42177..42338)
                     /locus_tag="PGA33_000124"
     CDS             complement(42177..42338)
                     /locus_tag="PGA33_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HDG6471966.1"
                     /translation="MSNIYKSYLLAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            complement(42331..42552)
                     /locus_tag="PGA33_000125"
     CDS             complement(42331..42552)
                     /locus_tag="PGA33_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471967.1"
                     /translation="MIKNSLQAKELAVILSVSKSKAGQIIRELNKELEDEGYIAIRGR
                     IPVQLARKKFPYHDLSDERIMEELKKENE"
     gene            42623..42868
                     /locus_tag="PGA33_000126"
     CDS             42623..42868
                     /locus_tag="PGA33_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471968.1"
                     /translation="MSYSEYEQLYYKIVNEADELYGGQSEHFKKNLQKLTENADEGVS
                     SEKIYSTALHESLEYQRNFIFLELGKVLFSEVGKCLK"
     gene            complement(42824..43039)
                     /locus_tag="PGA33_000127"
     CDS             complement(42824..43039)
                     /locus_tag="PGA33_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001796056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471969.1"
                     /translation="MNTLYKTTLLITMAVVTWKVWKIERNTRKPVINRNDFSKESTAE
                     TIERHSDPDSGIKLLKAFSDFTKENLT"
     gene            complement(43070..43264)
                     /locus_tag="PGA33_000128"
     CDS             complement(43070..43264)
                     /locus_tag="PGA33_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471970.1"
                     /translation="MQAQNKKVIYYYYDEEGNRRLLSIGNLDTYLLADIKSRFGLYKK
                     AIPDLDNLYIQIDGIEFKLY"
     gene            complement(43281..44069)
                     /locus_tag="PGA33_000129"
     CDS             complement(43281..44069)
                     /locus_tag="PGA33_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDG6471971.1"
                     /translation="MQALQTFNFKELPVRTVEIENEPYFVGKDIAEILGYARADNAIR
                     NHVDSEDKLTHQFSASGQNRNMIIINESGLYSLIFDASKQSKNEKIRETARKFKRWVT
                     SDVLPAIRKHGIYATDNVIEQTLKDPDYIITVLTEYKKEKEQNLLLQQEIGELKPKAD
                     YVDEILKSTGTLATTQIAADYGISAQKLNKLLHEARLQRKVNKQWVLYSEHMGKSYTD
                     SDTITIVRSDGREDTVLQTRWTQKGRLKIHEIMTEFGYEANVTA"
     gene            complement(44085..44303)
                     /locus_tag="PGA33_000130"
     CDS             complement(44085..44303)
                     /locus_tag="PGA33_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDG6471972.1"
                     /translation="MQWNLIKLRKERKCTQEDLANLLNISTEGYRLKELGKHQFKNDE
                     MFIIADFFDENIGDIFLPTKYTKRKQTS"
     gene            44445..45164
                     /locus_tag="PGA33_000131"
     CDS             44445..45164
                     /locus_tag="PGA33_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDG6471973.1"
                     /translation="MDKKELAKFIGNKIRYYRTKLNLTQDQLGEKLNTKKATISNYET
                     GYRTPKQDDLFEIAHILNISIDDLFPTRNNKKNDITSIYSKLTPPRQKNVLNYANEQL
                     DEQNKVTSIDEYKESKLVSYIACGATGAGIGEELYDDILHEEVFFKEDETPSNADFCI
                     LVNGDSMEPMLKQGTYAFIKKEDSIKDGTIALVVLDGVSLIKRVDICEDYINLVSLNP
                     KYDDIKVASFSNIKVMGKVVL"
     gene            45234..45401
                     /locus_tag="PGA33_000132"
     CDS             45234..45401
                     /locus_tag="PGA33_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471974.1"
                     /translation="MKITNCKIKKETIVYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKLDGDKLN"
     gene            45541..>45859
                     /locus_tag="PGA33_000133"
     CDS             45541..>45859
                     /locus_tag="PGA33_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471975.1"
                     /translation="MEENKTLKEYLRKFLEGYKYVVENRYNYQFSSNPEAFPFMRKDD
                     YKISIFYLNQSFFEEPCIVVISNDSKLKEIYNFRNIDIKYLSKHFTSYIYDSKKYVEE
                     QSGL"
BASE COUNT        12805 a         8942 c         6700 g        17412 t
ORIGIN      
        1 aaacttatct tttaattctt ttttatctac aattggataa acaaaaccgt ataactgatt
       61 attatcattg aaaagtaatt gtatcggttg ttgtgtaacg gatgcaacga aacggttatc
      121 atcatcagga actaaagttt ttataactga actatgcttt ttatgtaatt gactattaaa
      181 gtatgcatag taattactat aatttgattt catatatggt gctaattttt taaaactatg
      241 cgtttgatat aaacctgccg ggttaaaata ttctaattta tctttactaa catagtaagt
      301 cttattatcg gcggtaacac gataattttt actattttcg ccagtaattg taactacact
      361 atattttgga attgtgactt gtttatcccc ttgcatatcc acactgccat taaaagcaat
      421 tccataagct aacttttcgc catatggatc tttattttca ttttgcatcg tagcacgatt
      481 cgtaaaatct gttatcgaaa taattcctat attattgaaa atgagcaaca tcatcaatgc
      541 taagcctaaa atagtacctg aggccaaagt ccaaaaacgc actaaaaact taggcggttt
      601 tgcatgatta tagcgctcaa cacgtttgaa attataggtc atctctggta ttcttgtacg
      661 attctttttc caatctaaat caaagttttc aatttcttct tttgagttta gacttaatcg
      721 atcacgttca tgttctttaa aagatggaat aacttgttta agtgaccctt ccattcttaa
      781 ttgaccatgt gaaaaccaag ctatgtagtt actcacttgt gaaattttat caatatcatc
      841 accaattgac acaatcgtta aattattttc aatataatca tttgttaatt caatcgcacg
      901 ctccataaat tgtggtgtta aatagtcaat aacatgattt aaaataataa tatttgattt
      961 tgatgagcgt gcaatactta atagtaattg agcgtatgcc gctttcgaaa tatggttaac
     1021 cggcttcgtt ttataatcac ctaaatgtgc atattgaata atctgttcag ctttatgatc
     1081 attaatttca tatggaaata gttgaactaa ctgcgctgta taagcttcaa cagtttgatg
     1141 aattaacgat tgatcttcaa tatatccgta gaacaaatct tcagtacaaa ctactttacc
     1201 tttatcaggt ttaattgcac ctgccaacaa ttgacctacc aacgctttgg aagattcagg
     1261 ttcaccaatt atacctaatg cttctccttg ataaatatgt aaactaatat tgtttaaatc
     1321 gatatcttca gcatcatatc caaaaggtaa ataccatttc ttattctgtt tattcctata
     1381 gtagtgtgtt acttttagta actttaaaac aattgaactt cccatctatt ttcatccttc
     1441 tataattgta ataacgctat aggtaatcct tcttcgggtt catcgctatt aaatctaaac
     1501 ccccacgcaa aaataccttt taatctttct actttaaaat attgattact gccatcagtc
     1561 agtttataaa ttttaccaat ttctatcttt tttctgtcta ctaagtaact ttcggcaaca
     1621 attactttac tttgataaac gtcatattca tttaaaatac cattcatctc agcttttctc
     1681 attttctctt tataaagttg tatttcgtgt cttaattctg gctcagacat ctcactcaat
     1741 ttcttctgtt ccatcggcaa taccactttc ttctaattta gctttaattt tatcatattt
     1801 atatcctttt ctcataaggc cttcgatagt ttttgaaatt aatttctgtt gcgtgtactt
     1861 ctttcgattt ttattataaa ttttttctaa atctcgttgt aataaatcgt ctaaaaccgc
     1921 ttcatcttgt gtaaaatcca tttcattcaa tacagcatga atggtttcca tttcaaaccc
     1981 tttttgaatt aaagattgca ttactttttc tttaacttta ttttgtggcc ctttttttgt
     2041 tttagatatt ttttcagcaa tttggataat atcatcaagt tcctgttgtt ctctataaag
     2101 ttctgtaaat atttcaatga tatttggttc tataccaagt tgatatagtt tttgttgata
     2161 aattttaggt cctttatctg tcgtgcgaat cattgtattt tttaaacttt ccgcataatc
     2221 ttgatggtcg attaactttt cgcgataaca atattcaatc acttcagaaa tcgcttgctc
     2281 tgatatctct tctttttgta aatattgtat aacttctttt tcagttctct ttttatatga
     2341 taaatattgg attgctttat ttaaacctat gcgataatga tcatactttt gaatctctgc
     2401 catgtcagca gcttcaagtt gttgcccttt ttttaaatta aatttgacta atgtatcaat
     2461 atctataccc atttcaaatt gttcgtctaa aaaaagatta aaccgttctt tgtttttctt
     2521 ttgaacttct attttagtaa tcttcggcac tggatcaccc cgttactttc ttaagtttta
     2581 atacaatact cttttattat tcaataagcc acttcctata gcaaatgttt aactttaaat
     2641 atttttcgat gctaacaaaa aatcacacta tcatctttta aaatgaaagt gtgattacaa
     2701 gcaaatctgt aaaatttata aagcagaaac aattcaactt tatcattatg acatttcaat
     2761 taaaccttct acattatagt tccaagcatc ttacacatga atgcaagtat ttaacgattt
     2821 aattgtgaca tagcctgttg atattgtgtt tcattgatat aattttgttg cttcattttt
     2881 tctaagttcg tgcttacacg ttgcgtaaaa ttctctgaca tattattgat attatatacg
     2941 ctaggtgcat tgactttact agctaaaata gcgctttgta aaactgttat gtgagacatt
     3001 gttgtactat ttttattcac ggttgttcca aagtaatggt ttgctgcgcc ctcaagcgta
     3061 tattgattat ccccaaagta aatattattt aaataaaagc ttaaaatttc gttcttatta
     3121 tattgttttt caactcgatg agctacaaat aattctttta cttttctagt aaatgaacga
     3181 tcattatcat aaaaataatt tttgacaact tgttgtgtaa tggtactacc accttgcaca
     3241 tctctgtcgc taatcgttga aaataaagct ctagttgtac ctttcaaatc gaatccatga
     3301 tgattgtaga atcgttcatc ttccattgaa ataaaggcac ctttaacata ctctggcatg
     3361 ttatcagctg acacaaaact acttttattt tcaatttttc ttagttcatc cacattatcg
     3421 cgtgtagata aaaaatacat gataccaata aacaatgcga taatgattag aatggttaat
     3481 aatattttta atagtattcg tttacttttt ttctttttcg gcggtttgcc aactggttga
     3541 taatacgtat tatagtgagg ttcgtgtttc atatgctcaa aatgttcatt tgagtttgag
     3601 tacctatcgc ttcttttcat gcgtttgctc cttcttttaa aactcactta gtatatacct
     3661 tgagtttacc agtactatca caaataggct acactttttg ggaaaatcag tccaagggct
     3721 tacaatcgta tacgccatca tacttacttt tttgtttttt gaaaaaatta tagataaatc
     3781 attgcaattt taaatattaa tcatgtcaaa tattgttata ttttataaaa ataaaagacc
     3841 atccctatta aatgccaata gagacgacct tttatttgtt attcatttat taaaactaaa
     3901 atccatattt catttcaaac gaaaatatat aaattttaac aatcgataac cacaatactt
     3961 ctattgtaat tgtttaacga tttctcgatt aaaatcatct aaatcgtctg gtactcgact
     4021 tgttacaata ttgttgtcta caactactga ctcatcaact acatgtgcgc ctgcatttga
     4081 taaatctttg cgtacattta atactgctgt taacgtacga ccttttaaat cgtctgtatc
     4141 tattagtatt tgtggcccat gacaaatggc aaatgttggt acatcatttt tagtaaagta
     4201 tttagcaaat gtgccatatc gaccttctgt atctccacgt aaatgatctg gtgaaaatcc
     4261 tccaggaatt aataatgcat cataatcttc tggtttagct tctgcaatgc ctacatcgac
     4321 agtaactttt tcaccgtgtt taccaacaac ttcactattt gcagtatctc caatcactac
     4381 agtattaaag cctgcattct ctaatgcctc tttagggctt gaatattcta tatcttcaaa
     4441 ttcgtttgct agaataattg ctactttttt agtcattgca aatcaccttt ctatatatca
     4501 ttgatataat tactatagac aagtaaatca gtgattaaac atacaagata taaaaaatat
     4561 taagcgactg tcgcgatatc taaccctaac acatcttatg tggcatttac ttagatacta
     4621 atttaacctt ttcttcaagc tgatctaaca atccaatcca ttcatctata tcttcaacac
     4681 gtacttcatc aggatttaca tgatcgatat cctcaataaa cttatttaaa cgcgctttta
     4741 tctgttcgat tgtttgttgt tcattcataa aaagttaact ccttttattt tgttttcttt
     4801 ttcattatta tcctaacaga aattgcgtta aagcgatata atcttagcta tatttatgac
     4861 attcaaatta ttttgacttt taaaaatccc cttttcaatt aactaaaatt aagagataat
     4921 ttgttacgag tgataatacg aagtggtatc ataccgatat gaaccaaata gaaagaagga
     4981 agtttaagac gatgaatagc gtcaaattga agcaacctgt tagcatttac aatgatccat
     5041 gggaagtgaa atttatatac atttaaattt catgagacaa taaacgttga tttaatgcgt
     5101 ttttttgcct tttttatttt ccttattttt tctgttttac aacaaaatgg tatcaaaaat
     5161 ggtatcattt gtagttattt tagcttcaca tattaaaaca accacactcc taaattaata
     5221 ggtggtgtgg ttttgttggt tgtgtgggga taaaaataac cgcatcagtt aagatgcggt
     5281 tatctagcaa gggccacgta tttataaata cgtttagaat ctcttcggca actttgctat
     5341 agacagtcta tgctgttact aaattatacc accacacaaa cctactccca ttcaggaaca
     5401 cagagctttg tcgctcgtca gcaacgtcat atgaattctc agttcatgtt gtggtgacac
     5461 tttaaacggt ctgtgccagt agcgaccgag tcatttcaag aatgaccatt tcacatttat
     5521 attataacac ttgtcgtgcg taactgtata gtttttcagt tgtatttaaa gttaagttat
     5581 ctacttcgcg ctttccttgc cttaattgtg aaattacata ttgcgctacg ccagtttgtt
     5641 tgtgaatttg gtaacctgtt atatcacttt tgatcaattc aattattttt aatttataat
     5701 cactcatatt atctacgtcc attcttttta tctaaacaat aaaaatgtgt ttttctcccg
     5761 ataaataata acaatggtag gcttaataaa aacaatatta aatacatttg ttctgtcata
     5821 attgaaaacc tccaaataat attatattat ataagtgtaa ggaggagcca tcaggctcca
     5881 agcataatgt taatctttgt tgtttggctt tcggtctagg tagccgagat gccattctct
     5941 aagttgtttt aacacttctg gaattatcag tactgccaat acttgatgtt ctagaagtgt
     6001 ttttattatg tctagcatga ggcttttcac ctccttacac ataatttgta agtcatcaac
     6061 taacctacaa atataattat actaaacaat tgtttattaa gcaagtgttt ttttaaattt
     6121 gcataaaaaa taggcaagta ccgtagtacc tgcctgttat ctacatttaa atcttgagag
     6181 aaatgttaaa aagttctagt aaaataatag cacattttat ctttaaatgt aaatagaaag
     6241 caggtatgta acgcacctgc tcaaatagac atgactatgt cattctaact gatttctccc
     6301 cataagtcac ccaatatctg attaggtggg gcagaaccat tccatgttct aataggcaag
     6361 taataacgtt gcccctccca tgtatatcct acccaaacat gaccatcttg taacatcact
     6421 tctgtataat cacaatatcc accaggttgg aactgataac ccactggaca agataagaat
     6481 ggccccactt ttcttactgt gattggttga ttgccgtttg tgaatctagc actttcttcc
     6541 atgtagtaag taccatattt attacgtttc catgcacttg caactggttt aactgtatta
     6601 cttgaagcgc ttgactcatt agagacagtg gcaaccggta ttttaccatc catgtacgcc
     6661 ctaatctgct tgataaagta gtctttaagt tgcaaccgct tgtcttctgg caatagaccg
     6721 cgagttactg gatcaaaacc agtgtgcaat actgagcttc tgtgtgggca tgatgttgaa
     6781 gtgaattcgt tgtgtaatcg gattgtgtta cggtttgctg gtaatcccca ttttttcaac
     6841 aatctagcac attcttggaa agtcgcctgt tcatttttta aaaacgtcgc attatctgct
     6901 cccattgatt gacacacttc aataccgtaa ccatatttat tacctatttg attcgctgta
     6961 tgccaaccta cttgagattc atctaaggct tgccaaactg tgttacctga tacgtaacta
     7021 tgcgcaatac ctgcctctag tctcgataat ggcgcgttaa ctaatccatt acgatatgct
     7081 tctgctgttg ctcctttgct tcctgcgtcg ttgtgaataa ctataaactt agggttacta
     7141 ccacgcttag gtaggtcata acctttaacc acatctttga tgattttaag ttctaccgct
     7201 ttaggttgtg gcttagccgt ttccttttta gatgcttgcg taggagattg tactgatcgt
     7261 ggagctgttt cgcttttgaa gttaggacgg ataaaccaca tagggaagtc gtaagcgtgt
     7321 tggcgtcttg taactttttc ccaaccagag ccaggttgtt gtacaccgtc tgtccagcca
     7381 ccgccgagcc aattctgctc atatacaatg atataatcta aagttgcttc aattacccat
     7441 gcaacatgac cgtatcctgc accataatta ctaccgaata caaccatgtc gccaggttgc
     7501 gctaagaagt ctggtgtatt ttggtataca gtagctagtc cgttaaaatc attagcactt
     7561 gggatgtctt tggcacctac accttttaag ttgtagccaa ataagacttg ccaacctgca
     7621 ttggcatagt caaagcattg aaatccatac catccgtccg cattatattg ttttccctca
     7681 gatgttttca accactctat aaactctttt ttagttagtt ttgcttgcat tgtcgccacc
     7741 tccatgatga tactcattca catcaaagcc aacatcgtta gaggcgtctg tgaaaggttg
     7801 tgatgtatca tattcttttg gtgctttcgt gcttaattcc ggcgttaaac tgctgtcttg
     7861 tgatgatttc cacgtaactt gttgttcttc tttatcgcta tctctaggcg cttgatatgt
     7921 ctgtgctata gatgaatctg agacgccttt tgacgttggg tcagtaataa cgccaatacc
     7981 tgtaagtaac gtgaggatag cgcctataat tgcgctagct tgatttaatt gagtagataa
     8041 atctaatccg aataaatccg tgacttgctt gataaatagc aacaatgctc caactaaact
     8101 agttagtact gctttgtttt tgaatctcaa tttccagtta atatccattt gtttgctcct
     8161 tttatccaaa ataaaaaaac gactaaaaat tagtcgttta aaattattca atggtcaatg
     8221 tcggagatcc tgaataaaca tcacttatag tgacatacaa cgtccccgaa ggattactaa
     8281 agttgatatt tttacttgca actccgctat tgactcctga tattcctaat tcacttgaac
     8341 ctaaattagt ttgcgaaatc ctcattatac cgctacgtac attttctatt gtcacctgat
     8401 aacttttatt aggttcaact ccatttattg tccattttgc tgttgattct tctatgctat
     8461 ccggatattt atttttaggt aagggtttta ttacaaaata tgaaggcttt ttccatactt
     8521 ggatatttcc agcatatact tttgtatatt cttcgccttc gtaaataagt ttctttacat
     8581 ttttaaaatt accttccata aaaatcaccc cttaattaag taaagtgtat tagggtcttt
     8641 ttgatatata tagttatatt cattttctgt tcctgtccaa attttaaccg tcggttgaga
     8701 tgcgcttttt agttgatata aattatccgc ttgttgttta gtaaaagctt gagatgacaa
     8761 aacataccgc tcgtcatgat tatgattttt tggagcgtat aaatcattta gtgtttgttt
     8821 gaattcctca aaatcttctg cactaacttt tgagccaatc tgttgcaata cactttctga
     8881 aatagagttg ttttgtattg cttctgctaa ttctcttaat gtgttcatag attcaggcgc
     8941 gctatcaact agttcagcaa tttttgaatc cgtatacgtt ttagagtcgt tgagagttgt
     9001 atcttggatt tttttaactt cttgcaattt atcttctaac ccttcaacat ttgcgatatt
     9061 gattttatcc aataactcag gttctgcttt gatatctgta tctttaccgt caatttgcca
     9121 cattttagtg tcaggattga ttgatactac agtaccgttt ttaccgggtg cgccttgttc
     9181 tcctttttta cctgcttcac cttttgcacc aggttgtccc ggttcgcctt tatcaccttt
     9241 cgcaccttta aatctacttt cattcttttc gatgtaagaa atgacatctt tatctatttt
     9301 ctctttaaag tctttgttca ataaatctgt cgcgttatct tttaagattc tcgtaatagc
     9361 atcatctacc aatttaacat cgatttcttt tgctacagca gattcaatac cactatcaac
     9421 gatattgaaa gaaaagttcg cgacatgtat tttttcttct tctttctcta aaaacagctt
     9481 acagcgaaca taaccagcgt gtttgataac ctttttaggt atcttgtagg taatgaatcc
     9541 ttttacaaca tcatcgataa taaggggctc atttttgaat atagagccat cttccataaa
     9601 caaatgtaat ctaggtgtta agccgtgtgc ttttagatcg atacgacctt gtttgtcatt
     9661 gatacctatt cttatagatg ctgtattttc atcttcagtg taaaatcgac agccaatgtc
     9721 acctaagtca acaccatcat tttttattct cgtttcaaca tcttttattt tgtacattta
     9781 tacacctctt tatttatatt tatctcttat aaagtagata ccttttaagc cgattttttt
     9841 atatagctta gcgattgtac ttgcttgatg ttggcaccac tctatagcag tagcatattg
     9901 atgtgtagct ggatttttag gattccatct aattcggtac aatgtgtttt gccctttgtt
     9961 gatgtaatcc tttcttacga agctagcacc gcccatgatt gcttttgctg gagatgtcca
    10021 acctttattc ctagcaaacg tcattgcgta gttaggattg ttgtcgtaag cgccaatgcc
    10081 gaagtagttg tatactccat cttttccgtt agcgaagtta cttgttccat atccactttc
    10141 taagaaagca tgcgcgatta aataaatttc attaatgttg tgctttttac aagcttctgc
    10201 gaacgcttta ccttgattat tcaatgtccc cttaccttta agtatcttat taagcgaact
    10261 aactgaaacg ccttgatact tgcctaaatt aagcatttgg tagcactgcg tgttactttc
    10321 ccatattcgt ttaacattca ttgccgagcc cgtttgagct cgtgtagcgt tagcccagcc
    10381 ccaagcatta gattttttcg ggttacctct tgccatttgt ttatccagtg cttgtttgaa
    10441 tgtataaggg ctcgtttcag ttataatctg cggttgttta gatgccgagc cattgttagc
    10501 tgttggtgat gagtctctta cattcgctat atcagcgttt ttattatcta ccataacttt
    10561 tattctagat tttgttactg ttggtttagt tatagaattt aataattttt ctctgttttt
    10621 aaatatatta agtaatgcct tttctaatgc ttcgtattta tctttagggg caacaccgtt
    10681 gtcaatcata ttccaattaa catgttccaa catagaacgc caaatgctgt cgtctacttt
    10741 taaattttca atacttagag gtatctcata tttggccatc atatctacag ctacaaccat
    10801 tgcgtgaatc tcattaaaaa taaattcatt tttactcgca ctataatctt cacatacgtc
    10861 tataactata taatcaggtt cattaggaac ttcaaataca gctcttctag gtgcccaaat
    10921 attatgtcta tcaacataaa agtggggata ttccacatct tgtttgtatt tcttcctact
    10981 gttatataaa ttttctactg aactcattgt ttgagcattt ctaatcatta tccctttagg
    11041 tttttcgagt cgtcgattac cctctactat aaagtgataa atatattccg gataattaac
    11101 ttcttggcta gaaattgtgt actttatagt tgttacatct ttccaaattg gaactttttt
    11161 attatttttt tcgttatcat cactatcatc ttcgggttta ggtgccggcg tagatttctc
    11221 cggatgatat ggtggtctaa caaaatattt aacccctcca cctggtccat catgataaga
    11281 gtgcttaatt ttatatggcg gacttcctgt tgcgttattt gtataccagt tttgatctac
    11341 gccataccaa tagtcttttg tgcatggtcc cactacaatg tttacatgtc ctgcccaacc
    11401 accagtccaa acaccccagt cgcctggttg tggtacaaaa tcttttgtat ttctaattat
    11461 cttgaaatct ctacctctat aattggattt ttgagccata gcatcagcat ttccccatgt
    11521 tctaaacccc caatatttat cgagtaaata attaggtaaa tcccagcatt gtgctcccat
    11581 tccagaacca ggtacatcaa tagctatttt gtttttagcg atatataacg cccattcaac
    11641 cacttcacta gctgtaggct ttctagtctt tggattaggt aatcccatgt atgcacctca
    11701 tttcaatcaa aataaaaagc cagtgccgaa gcactgactc ttaactgtta tttacattta
    11761 ccaaaccaga agcacgccca gaagctatat cctaaaatcc ctttaagcat ggtaatcacc
    11821 tcctttaaat accaaaaata gttcttagta aagctatgac gatcgtactg aagatagtcc
    11881 ctaccaaacc gagaatccac atttttatgt ctctaatatt cttggcattc ttttctttat
    11941 tcttttcatc ttctaccttg tcgcgcttta attcttcaaa atttctatct aatttgtcat
    12001 aaatcttttc ttgcgctcta agactatctt ctattctgtc gaatttttca aacatagtct
    12061 tatcattttc ttctaatcgc gttaaacgcc aatcttgttc atgtcgtttg gtaaaaccaa
    12121 acattacgcc acctactttt tgttaaatta aaaagccaca agcattacac ctgtgacttt
    12181 tcatcttttg tttctggata tttttctcca gtgatcaatg catattcttc tttgtcaatt
    12241 acacccatgt ctacgtacca cttaatttgc tcatttttat agcaacccca cacataaaaa
    12301 gttttaatgt ctttaaaagt tggataaatc atcttcatca tttaaacgtc cccctcagta
    12361 tttgttttgt tagttttcag ttcggtcaac tgttgtgtta acatagcgtt ttgttgcgtc
    12421 aattgcattg tcaacatgtt cacttgcgtc atctgcattt gcatacttgc aaccattccg
    12481 cgaagttctt catcactcaa atctgattca ctttgttgtt ttgatgcatt cggtacgtct
    12541 tctttttcga aattgctatt gtatttaatt tcgccgttag tgaaaacaaa ctttctaggt
    12601 tcgaactctt ctttaaattt aataggcaca ttgttatcat ctacatctaa actattgcgt
    12661 aatccgccag tattaacgta tccgataact tcgtttttat cgtttactgt gattttcatt
    12721 atttccaccc cacaatttta tttatcgtaa ctctgtttgc attagcacca gaacctgttt
    12781 tactgcctaa atcaaggtac acatcgttat cgatttttaa cgtcgtacca ctttctttag
    12841 ttattaagca ttcataacta ccaccaccgt taccgtctga gtcaactaca tttgttttac
    12901 ttaattgaat cgcatttggt atagaggtta aactgaatgc ttcaataaca ccacctggat
    12961 aagtaccgct tatgaataga attgcataat ttgtataagc ttcggttaaa ttaatccttg
    13021 ttcctacacc gtttgcagca ccgtcgaata acacggctgt tttatgttcg ttaggtgcag
    13081 cccattgtga atctaatcga ccattggtga ttgatcgtgt ataaactttt ttagagtttg
    13141 aaggtgtgaa gttgaataac ttatttgcat catctttaac aaatactgat aagtagcctt
    13201 cgtaactttc aacaatacct ggtaaatcag gtacacttgt tacgtaataa ttcccagcgc
    13261 ccaatgcttc taaattacct ttggcgttat ataagttctt ttggattgat tgaccgttat
    13321 gttctgttaa tttatgttgt tgccaacttg tactttgaaa cttaccatct acatactgtt
    13381 tagcttgatt taaagcgttg ttagatattt cttcaacaaa ttgcttagtt aagtttccat
    13441 cattcttttt ataaaacggg taccacgtgc cgtagatttt gtattttgtg tactcatcgt
    13501 ttgaatcatc tgggtaccat gttgcacgag cagtattatt atcaacaaca taaacaacta
    13561 acacaccaga tttgcttgat gtataagttg attcatcgaa cgaagaaccg tcatcaacac
    13621 catcttgtcc aggcttctct aacgtgccta tatccgtctt ttctggcgca tctgttgcgt
    13681 tagtaatatg aataatccta gatgtgttaa ctgtgcttaa aacgctatct atggactgct
    13741 cagacgattc aatcgctttg ccataatcat cagtaatctt agacttttgc caattgactg
    13801 tcgagttgcc tttgacaagg tcagcaccat tgatttgttg ctcaacttca ttgacacgcg
    13861 caaatatcgt ttgctccttt tcaactattt tattgaattc agctgtaaca gcttgtgttg
    13921 cactagtttg agtcgcagta atagcttgta tagcttcgtt ttgcttgatt tcgatttgtt
    13981 gaatgccttt tgtcgcacta tcattcactt ttgctattaa cgtttgtgta tcagccatat
    14041 tttgctttaa ttggttaaag tctttaccga cagcttcgat agtatcttga atagatttga
    14101 tataaacaag ctttgttatg ccatcaaacc cactgactaa atcattttca atattgaaac
    14161 taaattgacg ttcaacaact acgttgttac tcccgtgttg cgtgaagaat gcctgagcat
    14221 gtaccttacc tgaatgtttt aaaaattcat ttggaatcac atattgcaag cgcccattaa
    14281 ttgcgtctac taccgttaat tcgtctgaaa tataagcgcc tctatctacg ttataatcat
    14341 cggtttttaa cacgatggaa gttttaacat gttcagaact tatagataac ggtctgttat
    14401 tcttagttac tgcaaaattt aaaacaccag ttcctctatc tgattcatag aaactgatgt
    14461 ttgtgtcaat aactggatta tattgtgatg ttgtttttaa ctcgattaag ttatcatctt
    14521 ttgaaaaatt atctactacc attattcaac ctccttacct tctattatgc tccaaccact
    14581 attaccacca gtaccaaagt ttctaacgaa aaactggtga gcagaagcaa agttattacg
    14641 tcttagcact tgtgttgtgt tacccggtgt atttgatttt acttctaaca cccagcctgc
    14701 aatacctttg taatctttag ggaagtcaga aaaacgtttt gattcttcag tggtgatata
    14761 gaagtctaaa ccaacaattt ttaaatcaga caatttcgtg atgctcttag ggatatgttc
    14821 ccaataacca gcactttgtg ggttaaaatt ccatgaaccg ttgtttttct tgttaaagat
    14881 gtcgataaca cgttcaaatt tgagcatatt tctacctgtg ctgtttctag ttagtacttg
    14941 tcttaacgca ccattataat gaccaggcag tacatcaaag aaccaacctg catctctaaa
    15001 cgctttcggt aacgggaaat ctaacgcatt ttgtgtgtct tgcgtataga tatagtaatg
    15061 accaacttcc gtaatatcac ttagatatgc tgggttctgt atcggtaacg gtttaacacg
    15121 tccgcctgaa tcagtcattg atacttgagg tgcaatgttt tttaagaatt ggtttacacc
    15181 tctttgaccg atagaataaa ttgagtgatg tctgttgtta cctggtccaa tagttacccc
    15241 aattaaaagt gctttacgtc ctgtttctag atcgtaatac atatctagac cctcagcctc
    15301 ttggaaatct cctttaaagt tgttattcac accgcctata tcgatacgac gtttaaataa
    15361 taactctttt gttttgacat cgaagccttg taagtaatta gggttggctg tattcgaatc
    15421 acctgtatac caatataaga tacctgcatc ataagtgata ccttgcatag gttgtgtatc
    15481 tgaagtgtat tccataggta tatccatttg atacaatact ttgtctatac ctttatcaat
    15541 atcgtcagca cttcttactt caatgaaatt caatgaattc ttagcttgtc tttcagaagc
    15601 tttatattca cgtctgaaaa tcattaaatt ttctatagga ttataaatcg ctgacgtata
    15661 tctgtcgtta aatatattcg gcatgacgtc ttgcatttca ttaccataag ttatttctcc
    15721 agttctatat tggaaacgta caaacttgtt gtttttgtta ctgtccaata cagctgaata
    15781 aatccataat tctccatcaa tgtatctata cgcattgtgt gtaccgtgac cgccgttttt
    15841 aacaagcaat ctatcaataa attgtccatt aggcttcaat ctagataaca tgtaatgatt
    15901 gcccggacgc gcttgtgtca tataaataat tttcgttcta gggtctatcc aaaatgattg
    15961 cattactgcg ttagtatatg gcgataaatc tgtgatgaat tccggttctt gctcttttgg
    16021 ttcaaatcgg tattctgtcg cttgatattc tttatagtgt tcatctacag ctttctcaac
    16081 ctttttagtg aaagtatcta gtgttgaata atcatgatac aaacgatctt gcaatgtctt
    16141 atgatcataa ccagtattat caacacgcgc gtcttttact tcgttgatac cgtcgccgtt
    16201 atgacctagt accatattgc tgaaacggcc gtttagatac gttaaaaaat cagagacgct
    16261 acttgtgaca tttaaatgct catactttat ttgctctcca ttatgtgcaa atacctcttt
    16321 atttctatgg tattcaagag agaaattaaa atccgtcagc atgtctgaaa taagtttaaa
    16381 gttatactca ttttcatcta catatctgta gtcaaagact ctacttaagt ctgtaattaa
    16441 tttgttatcc atgtcttcct ccttttctat ccgtaaaact ggtaataatt tttaataagt
    16501 tcgtacataa taacttcatg acccctctcg ttcggatgca atccgtctgg catacttgat
    16561 tttctgaacg ctggattata tggcttaaaa taatctgtat gataggcatc atatactggt
    16621 acatccaatt cactacaagc caatatctga gcattgacat aatcctctaa agttaaccct
    16681 agtttgtttt tgtccgtatc tttacggcgt atcgttgtac cactcatagg gcattgccta
    16741 gtagctgtca ttacaagtat ttttgaagct ggattatttt tcctgataac ttcaattgca
    16801 gaacaaaagg cgccgtaaaa cgttttagtg tcggttttat cagtgcctat cggtacgcct
    16861 gcccaataac catgtaacca gtcatcatct gtaccttgta atatgattag gtctcctctt
    16921 atttgctctg cttgtctata aatgctgttt tctaccgctt ctttacctat tggaactgtt
    16981 gccattgttg cgccacctct tgcaaggttg gtcgttttag cttttaactt cttgcctaac
    17041 atttctgtga aattagtttt cgcatgtgat cctctagcta cagaatcgcc aatcgttcca
    17101 attgttttta catctttaat gtttgattta tctataaaat catgaacgat agtgccatct
    17161 gatgtagtta cagtcttaga actcactttc tgttgtttgt cttcaattag atcagttcta
    17221 ctcatcaaat cgagtgttga tttagctatt gacgctactt tagacttcaa gttttctgcc
    17281 gctttactag gattagaaag gttaacatca tttaatccag aaacatagtt agctgcagta
    17341 ttaacttttt tcatatatcg ttgttctcga ttaaactcac caagcgttac atcttgctta
    17401 acaattacat tgtttatacc cctaatcgtt ttaacttgta ctatacggac taaatcattc
    17461 aaacctagtt tggtagattt tatttgtact atgtctccgg gttgtgggtc tgcttctgga
    17521 tatgattctc ttaacaccaa aaagtccaaa gacaaagatt gttttaacga ctttttcaat
    17581 ctcgattgta attctttatc catagtttct tggtcagtca ctttaccatc tttaaatggt
    17641 tctgcgtgga tgtcgccgta tatttcagct aatgcacttc tagcttccat tacgagccca
    17701 gcgtgttcga atgtttcttc tcctgaataa ttaccatatc ctctaatgaa ggtggcgaaa
    17761 tcacttgcat cttcctcgag ttttatagcg ttggcgttga cttcatcaga aataaaataa
    17821 gacgcttttt gatttgcaaa aggcgtcaat acaaacttat atctgtcttt ctttttgtca
    17881 tacgttattt tatattctaa accgaaatgt tctaacccct ttttaaacat ttctaacctt
    17941 gtgtcgcctt caccaccatt ttcaaacttc gaagacttaa ccttaccttc gacttcaaaa
    18001 agcattccag taccttgaaa cacaatgtta aaatatcttt ctactgtaaa agatcctgtt
    18061 acattaacat aaatcctatc aatcattaac ttgtctatag gaatctctct agcagtacat
    18121 tcaaccagtt gtctgtcgcc ttctgatttc ctatcaatga cagttattac atattctttc
    18181 ttgtcatttt caccttcgac atgactaaca atccatcttt tccctatagc gttaataact
    18241 tcataagtgt atttgttttc gagaatatca aaagttaata caccgtcagc attaactttt
    18301 ttcactaaag ttgtttctac tggtatgggt gcgccattgc ctttaggtgg tttaacagtt
    18361 atcgtcattc tgacacctac ttataataaa atttcaaatc gaactgaacc ttttgaactg
    18421 tttggttaaa ttcgaattta ttagctccgt atttaaattt tggttgggct atgttcgttt
    18481 cggtacttat ttcaacaccg tttttataaa ctcggaagct atcataaaca attttgtctc
    18541 cagcttttag tttgatacct tcgattttca ttatttcagc atgcgttaaa ttccatacaa
    18601 acgattctgt atcttcgccc aaaataattg ttatcttttt atacatgttg aattggtcgt
    18661 taggagcact accatgatag taaactgtat ctttgctaac attttcaaat gtatactggc
    18721 gcttatcccc acctgcatgc caatcaatat taaaatcaaa cgaccacaat ccaacctttt
    18781 tgttttcttc taactctagg cttgttccaa tactttcgcc gtatggtaat tctgtagttt
    18841 caaacttcaa ttcgaaagca actttgttac ctttttgttt aggatttata actccgctaa
    18901 aaataacttt atactgttta ccgtttacat aaatttgttg atcgtgtctt gaatattcat
    18961 aatccgggaa gttgttttta tctaatttca cgtaatcatc agaagttggt tgagtaaacc
    19021 tgtaattcaa ctcttctttt cttctgattt cacgtaaata cattggttct atatcagtag
    19081 ttaacgaata caacatatct cgcatataag caatgtctga acgatttttt actttacaaa
    19141 aacaaggaac aactatatct ctactgatat aattgctccc cattaatatg cgaccgttca
    19201 tattttcttt gtcttgatac tttgtgttga tttgcatgct atcaattact atatcgttaa
    19261 cgataaaccc gtattcactt aatttgatta cagtaccatc tttttttgtt aattctatgt
    19321 ccatttgtaa cctcctttat aagtaatact cagaattgcg tttagcattt ctgccgttaa
    19381 caatactagt aagcgcatcg ttattgacat cgaattcaat tttaacagtt ttcatgttcg
    19441 gtgatgtttc aatagaatgt gtgtgttgta cttgcgcatt tatatttcca cctaaattac
    19501 ttaagtttcc tgtaatacta gaaatgtcag gtgcgtttaa tgtaggttga aatgcatcaa
    19561 ctactttatc tgcaacatta gaaacattac ggataacttt acttgaatga ttatctatac
    19621 ctttaacgaa acctaacatt gaatacatac caacatccat gaattcacgt gaaggtgagt
    19681 gaatacctag cgctcttttg gctgcattta aagcaccttt tgctacacta gctgcttttt
    19741 cagctaagtc tctagccata ttaccaatac ctctcatcaa accacggatc atatcagcac
    19801 ctgctgatac aaagtcatcc acaaagcttt taactttatt tactgcattt gtcatacctt
    19861 gactaacttt gtttacaaca ttaacgaatc cttgaacaac tctattaaca aagttaatta
    19921 gcgtacttgt tatagtagat acccattgca tacctttagt gacaatgaag ttccaagctt
    19981 gagacatttt gtctgatata gttgatacaa cttgtgtgaa tatgcttaca actttattcc
    20041 aaattgtcgt taatatacca gataagaaac tccaaatcgt attccatata ttagaaataa
    20101 aactccatgc cgcttgtaac gcagtagata tagttgtagt gatagcgttc caaaccttag
    20161 ttgccacagt aactatagtg ttccacaacg tttgtaagaa cgtccaaata gcgttccaaa
    20221 ttgtcattgc gatagtcata attgtggtaa atactgtagt tattacagtg actaacaaat
    20281 tccaaatcgt agtagcgatt gtaattatcg tgttccagat tgtacttaag aatgtccaaa
    20341 tagctgtcca tatcgtcata actattgtca ttatcgtcgt gaaaacagtt gtgatgattg
    20401 taactaaaag gttccatact gttgttgcaa tagcgataat tccattccat agcccttgca
    20461 aataagcaac tatttgattc caaataatca ttataaaatt gtatacattt gatactgctg
    20521 tagtgatagc ttttaaaata gcattccata caaccgaagc tacagttttc aacacattcc
    20581 aaactgtaac cataaatgtt tttatcgcat tccaagcatt tataataaag tttctgaatc
    20641 cttcattttt attccacaat aaaacaaata tagctattaa tgcagcgatt acaccaatta
    20701 ctattgttat tggaccacct aaaataccaa acacagttac tagtcctgtg atagcatttc
    20761 taattaatcc aatcttaccg aataacaatt ggaatataac tgatataatt tttaatggtc
    20821 cttttaataa catgaacgca ccttttaaaa ttgttaatcc cgctcttaat aaaccgaact
    20881 tacttactaa tgcaatgatt ctacctatta atccgccacc cataaagtta gatacagcaa
    20941 gaataatcgg tattaaaaat ctaaatgcac caactaaagt tataatgaca ccaactaatt
    21001 gtgctgtagc tggatgcgcc tcaaacaagt tagctatcca accagttatt gcaactgcaa
    21061 cgcgtaatac tgcactagct ataggagcca ttgctgttgc gaatgcaact aatcctcttg
    21121 cgatgtttcc aatcaattgc attattagtg gtccatttgt ttgtatataa ctgacaaagt
    21181 ctttaaaccc ttgagattgt cctacttgtt cagaccattc cctaaactta gctgtcattt
    21241 gttcaagaga ttggaatatg ccagttgatg atccgctgaa tgcattcatc aaattgttaa
    21301 ttccaacgaa aacatttttg aaaatattac caatgatagg taagtttgtt tttgtgtatt
    21361 caataaaacg agttatcgaa ttttctccag ctgcactatt agcccagtta gagaaagatt
    21421 gacctaatct atccaaccaa tcagccgacc attgaaacag tggtgctaat tgcgtgaata
    21481 cattgactaa tccgtcacca aaatcacctg cagcacttaa tagcttgtta aataccgaaa
    21541 cacccgttgt attcatcata ttaaagaatc ttgaagctac actgctattt tcagcccatt
    21601 taagcacgct ttgagacgct tcttccattc ctcttgaaat accactaaaa aacggttgta
    21661 agctctgcat tgcagtttta acagtattta aaccatttgc aagagttgtg aagatagcgg
    21721 attgattttg ctttataata tcagtccatg ctgactttac gccatctaac gcttttttgt
    21781 attcgtttgt tgctgagcta gcttgtaaag tgccatcatt aagcatcttt atagcgctga
    21841 tagccattgc gccaaacgct acaaatcctg ctcccgctat tgctacggca ccacctaaag
    21901 caagtacacc accagttaac actttgatag cgtttaatag cgcaaatact acaggtacta
    21961 cgctcgctat tacaggtatt aagatactaa aagatgatgt aagtaatcca ccaaccatat
    22021 tagaacctac agtaccgaac acacggaaca tattagctaa attccccatc tgtctttgaa
    22081 aattgtcatt tgcttttatt atgtaggcat aagctttctt taaaccatta gtatcgacat
    22141 ctacctttgt tgtttttttg ttcggcaatg cgtctaatga ttttttaaac gcataaatag
    22201 ttggtataga aagccctgta tctacatcta gtcgagatct agttttgttt ggaatacttt
    22261 taagctcttc tttagtgcgt ttgattttag agttagcaac accgttgtcc acgtctataa
    22321 tagctttggc tttagaccta tttaatgctt caagactagc tttagatact tttaacactc
    22381 gattgaattt actgttatct gcattgatgt caatattgac acgcttcttt tctagttcgg
    22441 ataacttagc ttctgcttca gcgatatctt tagtcaattt ttgtttttgt aatttaatct
    22501 ctggagtaac ttctttagag tttagtttgt ctagttcaaa attcgattct agtacctttt
    22561 gttgcaagtc ttgtatacta gcatctaatt tagcttttac ttttttgtta ctaaaggcat
    22621 ctaaagactt tttagcaact ttgatagttt tttgtaattt tttatcatca gcatttaatt
    22681 cgacatcttt agtttgatct gctactcgtt taaatctttg cacagactta accgcactat
    22741 cgatttgcct tttgaatttg gctacactag cttcaatagt cgctttaatt ttatattccg
    22801 tcacattaac acctctcttt ctattgcttg ttaaattctg ctataacttt aaagaattca
    22861 ttattttgtg gttcgtattc atcacgttcg ctactaaatc ttatatcttt accttcgtta
    22921 agccgttgga tattttcttc ataaggcaat acgtcgtttg cgttgttaaa aacatattcc
    22981 tctttaggtt tattttctgt cccaacattt ttagtagctg cagcatcacg aatagcaaac
    23041 gcaagtttgt aacgttcgaa ttcttgggtt agcatttcat actctttcgc atacattcga
    23101 tagttatatt ctgttaatgt catttgctca ataacgttca aatctgtaat accaagtgtt
    23161 gacatacaag ttataacgat tctgtcgtaa gttattaggc ttccgctggt ttctcttccg
    23221 tttccactac ttcgactagg tttcgggtca taggtcgctt tcccaactcc gttaaaatat
    23281 ccgaaccaaa ttcttctagt ccaatatttt ctgcgatttc atctagtgct tcatcaatgt
    23341 tattaatagt aattgcttgt tttttcaagt gagatgtagc tgcaattaaa acttcgccaa
    23401 tcacaacagg atttccactc tctaaaccta caggcaacat tgatacacct tgaccgatag
    23461 aagcttgctc aacttttaaa cctaatcggt tatcgatttc tcttaaaaat ttaaaaccaa
    23521 aacttaattc taatgacttt ccgttaattt ctacattcat aacttaaaat ctccattcat
    23581 gattaattta aacaaaataa atagggctta acgccctatt tttatacctc tcctggtgta
    23641 accgttgatg aatctacctt aggttgtgga attgctgtta aatcttcgcc agttaacgca
    23701 tctgcttttg tagtgtcatg gaatctgtat ccagtcgcct taagtttctt tgttacagcc
    23761 tcaggtagtg ttgcaaatcc acgttggaaa cgaccattca ctccatattc atattcatat
    23821 tcatcaatac cgttagcttc tgcttttaat tcaaatttat tgtggaaacc ttggaaatat
    23881 ttcgctttaa atttagtagc atctccattt ttgcctggta ttctactttc aacttcccaa
    23941 gcctcataca atacgcgatc tacaactgca tcttcaattt catctgcaaa atcgtcacca
    24001 taaaacattt tagcagtacc agacattgtt gattcaactg aaccaccagt gttataagac
    24061 ccatccatcg tatcctctgt atctgtatca gcttcatgtg ataagccgta ttcagttaaa
    24121 aaaagcattt tagtagcatc tactttttcg ccagcttttc taaacaaaat aatacggtca
    24181 ttactatttt tcatattcgc cattcaatat tcctccgttt tttaaaatgt tttgtaagat
    24241 atcgttattg atgtgtgtag caattcttga ttagtagtat catcgactaa ctgtgcgatg
    24301 ttagtatcat cttcttcaaa gtcataatcg tttgttttaa cgctaggtgt taaatcatcg
    24361 atacaccttt taacaagtcc gtcatgatgt cctaaatcat cgcttacact ccaaatatca
    24421 ataactaaat tcgtatcgcc agaataacta tcaaacgtgt acttacttct atttgactcc
    24481 ggcattttta ttacaaaaaa aggatacgga atctcttgtt gcatctcttt acgagaaata
    24541 acagggaatc catatccttg tagcgtttca tacgctttat tataaagttg taagttcggt
    24601 gtcatgcttt tatctcctat tcaaacaacg ctttcaattc ttctacagtt gatttcctaa
    24661 tcacttcgta taccggccac ataaaaggtt cagcctccat gtatcgagta ccaaattcta
    24721 agaaaccact ataagctgca tgcgatgtga tagtgtattg caaatcgcca gtttttttat
    24781 atctgatatt gcgtgataaa ttaccagtcc aataaccctt attcattact tctctagctt
    24841 tcaatttagc tcgtactaca tattctttgg cgttttcctg taaaatatca tctacatcat
    24901 catcaatgtt ggttttcata tcgtgaaatt ggtttaacag tgcgtctaat ccatctatat
    24961 tcatcaattg acctcttcga tataatatga cgtttcgtgt ctgtatatcc ttgtatcaac
    25021 tatcttgtag cgaatgccat taaccaacac gtggctaaca gggtaagata ttgattcttt
    25081 tatcctcaga acacttatat cgttttttac atcaccaaat tcaagttgct ttcttgctct
    25141 agaaatgggg ttaatattgc atggtatcgc atcataagtg attagtgtgt tttctttttt
    25201 gctagtttta ggattgtaag ttgctacttg ttctaattga aaaataactc tatcttcata
    25261 tctcaaaaga acacagccct tcctttttta gttctcgttc tagcattaaa gtaattatca
    25321 ataatagctt catactcctt gaaatcgttc aattcatacg cattgctacg tccgtcaacc
    25381 gcttctgatg tcataccttc agcaccaatc ctgttgtagc gtttaactgc aacttcttta
    25441 atcatgtaac taaacctttc cggtatttgt tcaacttcaa taggtaacat tgataacaac
    25501 tggctttcac aactttttat gatttcttct aattgttcat cttgcttttc atctttaaga
    25561 ccaatacgtt tttttacatc agctagcgta gtcatataac cacctactct agtgactcaa
    25621 aagcattgat aatttcagct tttgtttgtt tttcatcaac ttgtaagcca gcaacacttg
    25681 ctatttcgac aagttctttt ttggttaatt tgtcatttac aatgtaaatc atttgtttgt
    25741 tgcgtttatt ttcaacacta gctaaagctt tgatacgttc atctgtagga ttataacctt
    25801 tacgagggta gacatgccct ttcatataga catgtctgtt atcttctaaa tctgtaaaat
    25861 ctactttaac aattccaatg atttcgggca tgttaccact cctaattatt tattaaactt
    25921 ctcctggaac tgaatctgtt cttttgtcag caggaactaa cttagcaaac gctttatcat
    25981 cagcaatatg caatgctaca tgcatagttg cacgtaatgc caccatgtct tgttcaaaca
    26041 agtttacagg tgtgccatct tcgtttttaa ctgtagataa ttgtgcagtt tcatcgattt
    26101 tgtattcgat taattgaggg ataccataaa tcaatttgtc gaagtcacca gtgattaatt
    26161 caccacgttt taagttgctt gatttaaggt taaccacagg tagaccgtct aacgaatcac
    26221 tgttacggtc ataaatacgt tctttcgttt caggatctac aattttacgt aacaagcttc
    26281 tgttttgtgt ttttgagata aatgcatttg cttctaattc gtcatcttca agcaatgcct
    26341 ctaaatcaat aatgttatct tgtgtgaagt cacctttaat aaccttatta gttttttcaa
    26401 ttgattgtgc aattgattta ccgaacggat tgttaccttg attcaaaata cctgcctcgt
    26461 caaacttttt atagaaagct tcagcaatca taggtttcat ttcttcaaag aattgtgaat
    26521 aagtgtaatt caagaattct tttgttactg gtaagataac ccctaattta aacgctctca
    26581 ttgtagcatt aacccaagta gccttagacg tttcgatttt ttgaccttca cctacccagt
    26641 aagcacctgg tttatcagcc caaaaagtaa acttcttctc agtaccttcc attggttcgt
    26701 acttacctaa ttgcatgatt ttagagtttt ccataacctc ttgtaagata ggtgttgtaa
    26761 agtcgtttaa caacgtgcca tctttctttt catgcatcat tacattgtca gggttaaata
    26821 cttgtggttt aacattgtta cttgcaaaat gttgcaaatt taattttaat ttttgtgttt
    26881 gttccattta aatgcctccg ttaattttta ataattcttt tttgtctagc tatttcagct
    26941 aagttttgcg gtttattttt agtcgagtga ttaaatgaat ctccaccagt caatggcgat
    27001 tgtctagcgt taaccttaac cgcttcatta accgcttttt ttactgcatt agaaaaagct
    27061 tcaacattca atttagtttg ttcagcagta tctgttacaa ctaaattgac aacctcatct
    27121 gatgaatcaa cttccgcttc actcaacatt tttcgtgctt ctgaacgcat ttcgtttaat
    27181 tgtttttctg aacgtaattg ttccagttct ttttccattt gttcgcgttc atattcatct
    27241 ttttgatcct tgttcatttt cgctaattta gcagcttctt tagcagcttc tgctgctttt
    27301 tcttttgcat actcatcagc ttttttcttt tcgtgggcta cacgacgttc aagtatttca
    27361 tcaactttct tttgttgctc tggcgtgaaa gttatttcag taccttcgtc attttctttc
    27421 ttatcaggat ttcctttttt accatctccg cctggttcgt ccggatcatc tgattggtct
    27481 gcaaaaaatt gcaaattaaa cttaagttta ttttcttcca tgagatatac ctccatttat
    27541 agtctgtcga ctgtttttcc atgcgtgctt tttatgtcat cagcacgttt tggacataaa
    27601 aaatagccaa cacaattaag tgctagctat taaaagagag gttcattata tttcgatttt
    27661 tctttatcgg ctaatactgc cgaccttaaa ctgtctaagt ttgcatcaat aataactgtt
    27721 tcgtttcgct tttgtaactc tttacgtata ccttttaatt ctcttgctat gtctctaagg
    27781 tatttgtcag tattactcat attagtatcc tccaaacatt taatttactg tcatacaaaa
    27841 ctaacttgcc tttaaaaaac ttcactttta aatcaatcac cgcttttcac tttccctccg
    27901 aagtattttg tttttcgttt cttgcttggt tttttcggcc acatagattt aggtagtaaa
    27961 gcgcaatctg aacgacaatt gatatgcata ggatagaaat taacaccaat tttagcgtct
    28021 ttaactttga atatttctcc attaagccct ttgcatactt tagttgttct attatcgatt
    28081 tttgcaatat acatataata tccttccggt gaaatttctt tcatgctgtc aatgcttgat
    28141 tgtgcgtgaa cacgtgccga ttccgtataa agcaatgatt taattgctgc ggtcttttgt
    28201 cgtgctgtgc cttcgaattt atttaagtgc ttgcgcatat ctttaacata ttcattagga
    28261 tgtcgacctc taataactac attagcaatt atttcttcta cttcttgttt cattgcttcg
    28321 gtattagtcc ataatcgctc tgaccaaacg acaccatgaa attgtgtatc aacgattgta
    28381 tctataactt ctttagctac ttgtacacct tcacctaaaa tacccgcttg atcactgaac
    28441 acacgataag ctgttgattc gaaatattcc ctcatcgata attctgtttg agctgttgca
    28501 taagcaatta agaattctat ttgaatcttt aacatctgtt ctctagatac atacattttc
    28561 gtgttatact tctttaattc ttcatttgct ctatcgctaa actccttgtt ttcgaccaat
    28621 ctttttgctt cttcttgaaa cgcttttaca tcgaactcat caataatctt ttgtgcttct
    28681 tgtaatgtaa cgcctgcaaa atctccgtac ttgacaataa acgcattgat ctctttttca
    28741 atgcgcttaa tcatcatatt caatatacgt tctatttctt cagctttagt tttatcacgc
    28801 ttcaactcat tctcgattgc tttgcgtccg cgttcttccc aatattcttg agtgtttttg
    28861 ttaggcaatt acaatcattc ctttttatca acagtatctt ttgtatcatc atcttgttcg
    28921 tcatcattga tgtctctagg gtctttataa ataccttttt gagctttttt aatagattct
    28981 ttctcatctt cttcgatttt cttaacttct aattcagggt cttggaagaa cgagaataga
    29041 gacattaaag ttgtttggct aatcttccca ccagaatcaa tataagcttt taattcttca
    29101 atcaatgatt taggtaagtt tctgttgtat acgtatctaa cagtattgaa atctttgtta
    29161 gcgtcaatcg accatgtatt tttaagtatt gtctctaaca acttagcacg acgtcttaac
    29221 cctttagtaa acaatccttc tttagtttta gtacgttgtt ccaatccaaa taatttgtat
    29281 ttcattgcct cgcccgattg agtgccgcta aagttatcat ctttcatgtt aggcgtgttg
    29341 gtaaacatgt gtatatcact gtttaaacgg tctttataag cttcggtacc ttgtacatcg
    29401 tattgcttat aaatataacc accatcaaca gagccttctg tttctctacc ttcgctatca
    29461 gcataaacag tcggttctaa aaacaacacg ttagcttcct tttgttttct aacttctaca
    29521 ggatctaaat ttaaattacc tttaataagt aacatagcgt catttaaatc actcatatag
    29581 ttagcagtat ctgattcagc attatcatac aaatcaatta aagtgattac tttctcataa
    29641 tccccttttc ttctttcgtt gttgctaaat tctgtaatag gcatacgttc gaaagagtgt
    29701 gattcaaaac cgttttcacg tggtgtgagc ttcaatccat ttgttctact ggtaagatat
    29761 ctataaacac cgtgtgaagt gaataaatca actgtaaaca ctccatcttc gtcagtcttg
    29821 tctattggtt tagttcttaa atatctaacg cccgcgatac tattacgttc aattgtattg
    29881 tcgtatatga caaaagtact cattgcatca ctcttgtata aacgcgtttc atcatcttgg
    29941 tttctaatca ttaactcata agctttgcca taaattgaca aatctaatcc taaagatcta
    30001 ttgtgtgact caacatcatt taaatcattg aacgcctcaa tagcttctaa tacatctttg
    30061 tcatcatctt gatattgaat tggattaccc aagaaatagc cgttgataaa atcgctaata
    30121 taagatgcgt aatcatgcgc tacacggtta tctgccatgt actcttcttt gcgtcgtgtt
    30181 aactcaacta agttcttagt tttaccttcg taataatcac ttaacacttt caatctaggt
    30241 cgttggtaat ccatgtgatg ttcaatgtat ttacttactt cattaacgtt ttgtaataaa
    30301 tcggattccg tcccgtcata tgtgtaaaca acattggctt catcattaaa taagtaattt
    30361 atgtttcccc gtagatctgt atctgtttca aattcgttta cttttaacat ttgttccctc
    30421 ctataatcct agagatttta ttgtgtcaac tttcgaactg agatttgtgc gttttctaac
    30481 cggtctgtag aatcgttcca ctgaataacg caacgaatcg atacaatgat tgtatgtatc
    30541 tactggttca ttggtatatt cacctgtatc tttgtccttt tgccatgtgt agttgtcaaa
    30601 ctcttcaata gtcttgaaac aacgttcatc aacaatgatt tcaaattgca ttaagaattg
    30661 taacccttgt acaaccgagc ccttcccttt tttggttggt aaaatccttt taagccctag
    30721 attccttaat tcagctatac ttttttgttc tgcactatct gctgtaattt cttctttagc
    30781 ataaccaagt tgctttatga cattagctat ttcatcattc agcatacctt gtttaacata
    30841 ctcttcaatg atgtataact tctttttctt tacatctatt ttagaatgta taaaagcact
    30901 aggatcatta acgtagccaa agtccaatcc aaaataagaa ggtaaatgtc ttaactcatc
    30961 tttatttatt aaacgttttt catacttagg gaaaaccaat ttgtctagtg tagcaaattc
    31021 acctaacgca taaattttgt aatatgctgg attacgattt gctaacaact ctaagttttg
    31081 tcgtgtcatt tcatcaagaa acttattatc tcgataacta gattgtctaa tcatgacatt
    31141 ttccattggt tcaccatgtt caaagaaata cttataaacc caattcagtt tagatactgg
    31201 gttaaacatc aaaaatattt gcttattcac gtgtttacgc tccctcaaac gcaacgttaa
    31261 ttgcgtgtaa tcatttagtg tgaattcaga cgcttcttcc atgactatgt ctgatatgcc
    31321 ttttatcgac tttattttct ctgggttatc taatccttta aacaaaaaaa ctgcgccgtt
    31381 tggcaattca actttgttat cagtcttatt ccaaaggcac atgtcccaaa taccaaagtt
    31441 tatcaaacaa tctttaacat cttcgaacaa actatcttta attgttgatt gtacttttct
    31501 aagccacagt atacgcctag gatatttcca atcttgcaat gctttgagta caactttttg
    31561 tataacgccg tgagacttac cgctcgaacc tccaccgtaa tgtacttcag tgaagttatc
    31621 gtaattggtt agtatttcga atatgtttct attgaaaaca ttagacggtt tgttaaagtt
    31681 taatttaact ttcgtcatcg tactcaccaa tattaatctc aatattcttc tgagtaattt
    31741 cttttttatc gatatacgca ccatgtactt ttagtatgtg gtcaatagat ctctgacgct
    31801 cttcaaaagt tggtgtgatt gtgtaagtaa cctctttttc cacttcatcg tttaaatggt
    31861 catatttctt actgtaagcc tcttgaggtt ctcctctagc aatagaagca gataacgcta
    31921 aagcttctgt aatactcatt aaacgctctt cttgtatctg ttctaatcgt tctttaatat
    31981 attccgaaac attaacattt cttaacaatc gacttgctaa agactctgct gttttcttac
    32041 tataacctgc tgtaattgct gcttttttac cattacatcc attcattata tattcatctg
    32101 cgaatctctt ttgtttttcg ttcatttcat ttaccaccaa ctctcgcgct atacgctttt
    32161 taaaattaaa aaaggattgg ctataatcag ccaacccaca tagacccttt attcctaatt
    32221 gcgataaggg aaacgcagta cgatagtcaa tatcctacac tatcataata tctcatttaa
    32281 ggtatcaaaa actgccactt tactgccaat ttcagtcttc ccctaactct tccgccaatc
    32341 tagatatgat ttttcttttg attctatgag cagttctatc agaaatgtgt atgtcaacac
    32401 aaactttcac taattccttt ttattaaaat aatactcttg aatgaattcg cgttctttcc
    32461 tgcttgatgt gttgattata cgttcaatag cgctcttaaa ctcaaggatt ttacctcttc
    32521 gtatactaca aagataatta gttactgcca tttctgtttt cgatgtatta gacggtacaa
    32581 actccccgcc tatatttgta tctgttggaa tccatggtgt cattatttca cttcttaaat
    32641 cttcgagttg cttatgataa ttaggataat cacacaactc atcttctaac tttcgaactg
    32701 ttgataattt taatccatat ttctttttag tcatgaatac cctccataca aatattttta
    32761 atcttcaaaa tgtctcaatc tacttcttaa tatctctatc tcccgctctt taactttcac
    32821 atcgcctttt aactgttcag cttgcaacat cacaccaaac aataagatga ctagtaatat
    32881 aattgctatg actaaccaca tcatctactc tgacacctcc gccctcatca aatcagactg
    32941 atcgctcaac tttgcgaagt cactcggcgc ctctacatca tcattagccg tcgtcataat
    33001 atatactttc tcagttacat acttacctag ctcatacatc gctagtaaga ataatagtct
    33061 taatatttgt ttaatcatta tttatctacc ttctttactt cgtataagac cggatataaa
    33121 tttaaaaagt gtattctata accaatcgtt ttaacttcta ctttgtcgcc tacttttaac
    33181 ctagcttgta tgtctgcgct atcaaatttc tttttgaata gtaagtcaga gttttcgatg
    33241 acttgcttgt tgtctaacac aatatagaac ttgtcttctt tatcttgtct cttgttatat
    33301 ttatctgtaa ttgtcccttg atgtacttct ttgttttggt aactagccac tgtatagata
    33361 ggcgatatga caacaagcat cagtgcgatt acgccgaata atcgcagtat tccaacaata
    33421 aagatatcga accaatccat atttttaagt tttttaatca tcattgtcat ctcctgtatc
    33481 aatcaaaaaa agtacctgtc tcaacatact ctttaactgt tgttcattta gactggctaa
    33541 catagggctg taaaattcac tatcttcatc tttaacagtt ttaataaaac agccttcaat
    33601 ctcagctttt tcttctggcg ttccattttt atacgtctta aatacctcgg tgtgcttttc
    33661 tggtaatttc attttaggtg tattaaacat tattatctcc cctctttaat gattttattt
    33721 cttttcgaac aaagaaccta atacttcttc actaggtctt tcgaataagg tcactttaga
    33781 attattagtg tagtaaacaa taggtgtatt ttgtgactca tatttctctt tcgcttcttc
    33841 tttactctct gcctcaacaa ctgtaaacct ttgattgctt ttagctcgag ttatgtgtgt
    33901 atgtttacgt cctgttgaat ctttgaatgt tgtgactaag tattgcgtca cttcttatca
    33961 ctcctttgaa tgattctaag tttttctacg aataaaagta ttagtaaaac actcaatgta
    34021 gctaacatat tttgttgttt tgcaaaatct actataacga ttaagactaa taacattcca
    34081 attctgcatg taaataaatc taattctttg tacaaaacca tatatctgtt gagtaaattg
    34141 ttaaatatta ctatgaatac aagtattaga actaatgtaa tgatgtaact cacttcccca
    34201 aaacctcctt gactcgatct aagatgtctt tacactccgc tacttccgaa gcctttttct
    34261 ccacgttctg aaacactttc gaattcctcc acttgcttta gttcaggtgt ccatataggc
    34321 acgataacca attgagctag tttgtcgcct tcgttgattt gataagttcc atattgtctt
    34381 atggcgtcac tcaaatcgat ttctccttta atatcaaaaa cacctggtgt aatataacca
    34441 tttgatgcaa tagcgtcatt cttgatgtta atccctaaat taccatgata tcccgcgtct
    34501 atcttgcctg tttcaatcac taaatgcgtt ttactgctta caccactacg gctagttaat
    34561 agtccgacat agccctctgg tatgctcaca gctacatctg ttttaatcac tgccttttct
    34621 tgtggctcga gtacgacagt ttcagctgag aatatgtcat aacctgcatc cgtcttatga
    34681 tttcgttctg gcattctagc gttttctgat aatagcttta cttgtaggat gttagtcatt
    34741 ttccttgtcc tcctcatcaa ttccagccaa ttcatacata acgctctcca tacttttatc
    34801 ttttaaatct tctgacacga atacctttaa atcatggaat tctgtaatta ttgttttttc
    34861 gtcatggtcg atgtaaattt ctagcgtacc atcagctaaa ttaaaaatag ctttatcttc
    34921 attttctttg tatccttttt cttttaattt cttcaaagat tcattaattt tcattttcct
    34981 gttcctcctc atattcaagc tctcttactt ctctcaaaac ctctgacgcc cagtcttctg
    35041 gatactcaac ccattgcgca ttaatagctt cgcgaatttc atcaaacgct tgcgctttct
    35101 tatacacgtc ttcaatctct tttagcaatc cctctgtgtc attgccgtta tacgcactag
    35161 cacttataac ggattgttct atttgttcac ggttatccat ttgtgtcatc ctccgtattc
    35221 ggataaaatt taataaacat tttattacca tgcttatcta tggcaattaa ctcttcgtat
    35281 tcgtcgtgtg atacatactt atcaatcaca caattttgta acatttgcat catttgcata
    35341 tgcttttcag cattcatcat ttccatctcc tctaaaataa agttagttgc ttctgctcct
    35401 cgtattccaa accatgttgc tttatatatg tttcaagctc ttccgctgta tcaaatatct
    35461 ttttcacacc ttgccaacct ggcacgatat gcccatgaaa gtaataagtg ccgtttacta
    35521 catggatatg tgccactcgc tcgttatcct gatacagata tctcttagat ccgaaaaatt
    35581 ggtttaagta ttctttgcgt gcgttatcta tcatggtcat caatcccaca agtcaaaggc
    35641 tctttggacg taaaacttcg cctttgctaa atcctcatga ccgttcttta acggtgctct
    35701 agacaggtat ttgattgcat tacctattgc gaatgctaat tgtggtgggt actgtgccgt
    35761 aacttgttcg ataaaatcta taatttcaat gtcgccgtat gtgtaatgtg caggttgctt
    35821 aacattgtct tgcgttttgt tcatatctac ttttctgtta cttattacgc tcattatgct
    35881 tcactccatt tcttgaacat ttggttataa gtgacatcga accagtacgg atcacgtgaa
    35941 tgtttccgag gtacattaaa caaatgtggc ttcttcttac gtagctcagc ctctttcttt
    36001 cgctctcttt ccaattcacg ttcgagtctc gcttgtttaa tcttttccat ttgtttcatt
    36061 tctctatatt cttttaggtg catgccataa ggcgcgtcta aagcttctga aaactcccaa
    36121 caacctctaa cacgtttaga aacaattcca gcgtttatcc cgcgctttgc cattagttct
    36181 ttttcaaaat tgttaaattt atatggttta ttattaatga ttacaacact gcccatttat
    36241 tccacctcta catttacatt tctaattttt aaattgtcat actctagtat ttcgtcagga
    36301 ttgttatata agtaatctgc cagcgtttct ttttctttat ccacatcatc aaaatgctga
    36361 tattcaactt cggtaggtat tcttatatca atcgttgcgt ttatatatgc ttgttgttgc
    36421 attagatcac ttcatttctc ttttgcgttc tcgtcttgct ttaattaatt cctcgtacgt
    36481 aatccatgtt ttacctgtat acttaggcgc tttacatatc caattgagtt ttatgtttct
    36541 gtatttgtgt ctgaacatct tagctttaag ttttgctact tcggttggca tacctttaat
    36601 gtcgataact tcaatcagtt tgtcatcgag atataacgcg aagtctgcaa tatattcaat
    36661 ctttcgttgt ttatctagtt ttggtaataa ttcaaatttc ggttgtagtt cgatacgatc
    36721 atagttagtg ccattcatat tactttctaa atattggtaa tattcacatt ctactttgct
    36781 gtcaaataca atccctttat actcaacttt cttagcgttg tatttactca tcgtgcacct
    36841 ctaaatatca aatatcgttg cttgtaatcc tagttcttgc tcatatagaa gcccgtgagc
    36901 gcctttgaat cgttttaggt cactatcagt cataattttc ttttcgtcgc tgaaatgggc
    36961 tcctgtgagc gaataaactt cattctcgtt atcttcatgt ttgatgacct taatatcttc
    37021 cgtgccatct tctcggtata agtaatattt ttctttcggc atttttaaca ctccttaata
    37081 ttcgacgaca gcggggcgtg tgtgacgttc tgcaagtttt tggataaata ggtcgtacaa
    37141 cctattttca tcgccctgtg cctcgtctat gagtttctga gcataaatat ctgaacactc
    37201 aagtttagtt tttaaaaatt ctttggttac catgcatctc gctccctgaa atcgtctccg
    37261 attactctta cttttctcgc attgtgtttc attcttgaat tgatacgttg ccagttcata
    37321 ttttgattta gttctttatc actaaagtta gttgtaaaga tgttgttttt acctactctg
    37381 ttatcaacaa tgctgaaaag tttatttaaa gtgtgctctg tgttttctac acccatatca
    37441 tctagtacaa gtaaatcaat atcacttagc aatctgacta gctcgtctgt agtttcaact
    37501 gcatttttgt tgtatgtcgc tttgatacga tccatcaaca ttggtatgtg cataaaagca
    37561 actgtatgcc ctttagattt gactgctttt gcgatagcgt atgctaggtg gcttttacca
    37621 gttccatatg aaccttgcaa tattaatgat tttggttctt ttgtagagaa accctgtaca
    37681 tactctattg ctgtttgttt agcttttact tgtttttcat tttgtggctt atagttgtta
    37741 accgttgcat ctcttaatga cggattaaca tttgattgat tgaaaatata atcaagtttc
    37801 ttttgtttat tccttttgta ttcttcgtaa gccaatcttt gaatttcaca ttcgcaaccg
    37861 tctttgtatt catatccatt ttcaaactta tataagtcat attgatgccc gcatttatcg
    37921 caattctgtc ttagtattac ttcgattggt tgatattttt ttaaactttc gtttattttt
    37981 tcgttgaata acggtttcat aacatcctcc tagtcccaat aactttcgtc gtacttcata
    38041 cgttccaatt gatctatacc agtttcttta atctcttcgc tataatcatt catatagctt
    38101 tcgttagtta aaaacgtttt agggtacttt tgatattgtt tgtctgtaat agtttttaaa
    38161 tactctcgag taccttgcat gatttgctca aaagtatgtt tctttacgca tgatttgaat
    38221 ttagtgaaag acatcttctt atcttttttc ttgttgtaaa gtttccacca ttcctcaaat
    38281 tgctcatgcg taacgtcagt tgcgctatta tttgaactta agttcttatc tatttcttta
    38341 tctctttcta attctttatc taattcttta tcttcttctg ttgcgtgact gtcacgtgac
    38401 gtcacgtgac catttagcaa ttttctgttg ttttctcgtt gcttttgttt cctcaacctg
    38461 ttctgagccc tgattttctc gagtccttca atgttttggt gtttttccca gtttgtcact
    38521 tttatgacac cattaacttt ttcaatcatg cccaacgtct caaaagtttg tattgctaac
    38581 cttattgagt taataggtcg gctaaactca tttgctaaca tttcttcgtt atacggcaag
    38641 ttttcagata acataatgta accttgttcg ttgtactttc ctgataaagt tagcaactta
    38701 acccaaatgg ttatgatcgt atctctttcg ggtaaagctt cgatatattt gattttgctg
    38761 tcatcaaaca tgccaacttt aagttttatc cacgatactt ctcccattgt cttctccttt
    38821 cagcgctttt attttgtccg gtacttccca gttagatatg aattctttaa gttcatctgt
    38881 cataggtacg tcgttaagga tcgcgtcaga tccatgcagg tatgacgaac atttgttgta
    38941 aactaatctc gctttgttta aatcgtcata tccgcctaac gctatatagt tgccagaata
    39001 aaatattttt gaataatatc tatgttttat tttgtttatt cctcttaaat tgtttttatc
    39061 agttcccctc ttcaattgct ttatgtttgt tttatagttt ctttttttca gcctattatc
    39121 ttctccaatt atattaaggt aaccaacacc accccaatat tcattaactg cattgttgta
    39181 agcttttgct gcttcatctt catttacaaa gtgacctaag tttttggttt ttttatcaac
    39241 agctatacat gcataccaat tattattttt tttatcccat gaaacgcctt tatatttaga
    39301 tgaattgtta cacttcgctt tgctccatct tgttttatta ccttcagttg ttagattttt
    39361 tcttgtgaaa tcattgtttt ttattttttg gaaacttttt tttagaataa aatcaggtaa
    39421 atgcttttta tcactattca caatcattct gtaattatct ttaaaagctt tatgccaagt
    39481 atgctgatta actctctcgt aatcttcatc atcaactaaa atttcttctc catcttgtaa
    39541 aaatatcgat ttaaccatta ttctcctcct ttcaacattt tattgagcct ctcatcaact
    39601 tttatccacg agtcatgcaa gtgatattta tcatcaaacg acttaacgcc aatcgcatgt
    39661 tgctcgttgt gatgttcgcg acataacgct aatacatgtt tgtcatagtg attcatcttg
    39721 tttctgttca tgcctctacc tactgcttcg taatgtgcta ggtcagcgtg aggctttccg
    39781 catattacac aattgcggtt gattgtagcc caatataata acgctttatc ttcacttaac
    39841 aacttactcg tttctacact cataggtatt tgatgatgaa acataaacgc tataatcagt
    39901 tctattaact cccttgcgac tttcattgaa cagtcacgca gactgatttc ctcataacct
    39961 ttttcaattt ccaattctgt ttgtaataat tttctagttg attccactgg ttcgccccag
    40021 tgaagttcta tatctctaca cattgcgaat atttttttac gttgttctat agataacttt
    40081 ttattgtccg gaacctctac ttctgctttt agtggatatc cgttttctag caagtcaatg
    40141 tgactttgtt caagttcaac accagtagca acgacggaat aagtgccgtc attgtctttc
    40201 tggtatcttg taatgtattg catttaaacc acgtcctaga acggtaaatc atcatcatta
    40261 atttctattg gaccattagc attagcgaat gggtttgatt gttgactcat tggcgtctgt
    40321 ttcccatttg cttgctgttc tttttgtttc atctcatcag ttttaggttc tggtttatta
    40381 actacttcat cgtctttatt ccaaactttt acatatgaga gtcttacaaa atacttgcct
    40441 tgttcctcgt taaatttatt tttaagtaca atagttccga ttttgttaat taattgatct
    40501 gtgtcaaaag ttaaatctgg taagttcaat ttaattccta atctactaag taactcgata
    40561 tattgttttt cttgataatc ttgttggaat ggtgggacga attggttgtg tttgtattgt
    40621 ttaccttcgt tgttttcaaa aacaatcgtg aagtatctgt tttctctgtc gttaaactcg
    40681 acatttgcaa cttttactgt aaattctcca gctcctaaaa agtccccacc tttcatgaat
    40741 gcctcttgat tagtttcttg aatgtattgt gttctaccag tgattttcat aatttttata
    40801 ccgtcctttt aattagtttt taattaccat ttctaattgc ttgtacaaca tcgttaatac
    40861 ttggattaat gaaacgtttg ttgttaattt tgatgttgct tgagtgtctt atctttgtct
    40921 cgaataagtt tgatggttca gcgttaagta catattgata agttttttcg ccgtcttgct
    40981 catgttcttc tattgtcatt cttgctaaca cgtcagattg actgatgact gcttttttta
    41041 tttggtcttg tgcctctatc gtgattgttg gattgatagt acttccctca tcatctttgt
    41101 ctttgttaat gccctcgtgt ccgcttatag caagatgaaa ttgataatgt tcttgtaatt
    41161 tagaaatata acgataaata cttacaatgc gtgtagcaca ctcgccccaa tcattaaatg
    41221 tcggtttctt tgatttaccg tccatgatgt cgtccatagt gatatcacgt aacttttgga
    41281 ttgtttcaat cactacaaca tcaatttgtt ttccgttttc tcttagttgt tcaataattt
    41341 taggcagcat tttaatcact gcactaaaat gcttataatt cttaatctgc acaactgccc
    41401 catcttctgt taccgttgtt ccgtcctcat ttatatctag tactaaggca ttgttatctt
    41461 ttgttaaaaa cgtagtttta ccagtaccga acttgccgta tatcgcaaat ttataaaact
    41521 tgtttgcatt ttgtttgctg atgtctttta cacctagttg cgttaaaata tcgacatctt
    41581 gattagtttg ttcagtcatg ttctacctcc tcgtactcaa ttgtttctgt cactgttttc
    41641 ttgattgctt tgtgataatc catattgata ctcgcttctt ccataccgtt aaactcccta
    41701 gctctatttc tatttgtgga gtaactaata tctgaattgt tatcagttgg tttgttagtt
    41761 atataaattg gcatatccct atgacgaatg atataagtta cagtctgctt catagcaacc
    41821 tcctaccatt tcatgactaa gttaattagt ctgtcctgtt cgtctgtgtt ctcttcaatc
    41881 cattcatcta ttgcttggtt aaataagtct gatgccatat ctaagtcatt ctcatctacg
    41941 acataagcat gtttaattgg tacgttgttc atatctttaa cttgtattga tatgcccata
    42001 tgacctttta aaatgaatag attaaaattg aatccgttaa catgaatatt tttgcgtatg
    42061 atttcgccta tttcgtaata catcttgact tcctccgttt ttcgttttat attgaacacg
    42121 aattaatttt gttaatcgtt tgtcactgtt acttgttggc gcaagtagca gtttttttat
    42181 tcttcataaa agtattcctt atagaatatg aatgttgcga tacttgcgaa tcccgcaatt
    42241 gaccatgctg tagtgaagta tagaaacggc atgagtacaa tcgctaagac cgtgaagcac
    42301 agtactgcta ataggtagct tttataaatg ttactcattt tcttttttca actcctccat
    42361 tattctctcg tctgataagt cgtgataagg gaattttttt ctagctaatt ggactggtat
    42421 tctgcctcgt atcgcaatgt atccttcatc ttcaagctct ttattcagtt ctcttattat
    42481 ttgtcctgct ttggatttag aaacagataa aattaccgca agttctttag cttgcaaact
    42541 attttttatc atatctattc ctccttttta tttttgtgtt gtgtataatt tagttatctc
    42601 ctagtgaaag gaggtggata atatgtcata tagtgaatat gaacagcttt actataaaat
    42661 tgttaatgaa gctgatgaac tatacggtgg tcaaagtgaa cacttcaaaa agaaccttca
    42721 aaaacttaca gagaatgctg atgaaggtgt ttccagtgaa aagatttact ctactgcttt
    42781 acatgaatca cttgagtacc aacgaaactt catcttctta gaattaggta aggttctctt
    42841 tagtgaagtc ggaaaatgcc ttaagtagtt ttattcctga atcaggatca ctgtgtcgct
    42901 caatcgtttc tgctgtagac tctttactaa aatcatttct attgattaca ggctttctcg
    42961 tatttcgttc aatcttccaa accttccaag tcacaactgc cattgtgatg aggagggttg
    43021 ttttgtataa cgtgttcatt tgtttatgct cctttgcatt tccaaaaatt taatataatt
    43081 taaattcgat accatctatt tgaatgtata gattatctaa atcagggatt gcctttttat
    43141 ataaaccaaa tcttgatttg atatctgcta ataaataggt atctaaatta ccaattgata
    43201 atagtcgtct attaccttct tcgtcatagt agtaatagat gacttttttg ttttgagctt
    43261 gcatttgctg tgccctcctg ttaagcagtt acgttagctt cataaccgaa ttcagtcatg
    43321 atttcatgta ttttcaatct gcctttttgc gtccatctag tttgtaaaac tgtgtcttct
    43381 ctgccatcag aacgcacaat tgttatagtg tctgaatctg tgtaactctt gcccatgtgt
    43441 tctgagtaaa gcacccactg tttatttact tttcgttgta gtctagcttc gtgtagtagt
    43501 ttgtttaact tttgtgctga tataccgtag tctgccgcga tttgagttgt ggctaatgtg
    43561 ccagttgact ttaagatttc atctacatag tctgctttgg gttttagttc tccgatttct
    43621 tgttgtaaaa gtaagttttg ctctttttct ttcttatact cagtcaacac tgtaataatg
    43681 tagtctggat cttttaatgt ttgttcaatt acattgtctg ttgcgtagat accgtgtttg
    43741 cgaatggctg gtaggacgtc tgatgttacc catcgtttga attttcgagc ggtttctctg
    43801 attttttcgt ttttactttg tttagaagcg tcaaagatta aactgtataa tcctgattca
    43861 ttgataatga tcatatttct gttttgacct gatgcactaa attggtgcgt cagcttgtcc
    43921 tcgctatcaa catgatttct gatggcattg tctgcccttg catatcctaa aatttcagca
    43981 atatcttttc ctacaaaata aggttcgttt tcaatttcca ctgttcttac tggtagctct
    44041 ttaaaattaa atgtttgtaa tgcttgcatt gttcgttcct ccttttaaga tgtttgtttg
    44101 cgtttcgtgt actttgtggg taaaaaaata tctccaatat tttcgtcaaa aaaatcagcg
    44161 ataataaaca tctcatcatt cttaaattga tgctttccta attcctttaa acgataacct
    44221 tcagttgata tattcaagag gtttgctaaa tcttcttgag tacactttct ttcttttctc
    44281 aactttatta aattccattg catgttgtca cctcccgctt acaaaactaa ctatacacga
    44341 tacgtgtact tgagtcaaca taaaagtttg cttttcgtgt atttttttgt tgaataccaa
    44401 aaataattgg gttatactat aggtaaattt aaggaggtaa gaaaatggat aaaaaagaat
    44461 tagcgaaatt tataggcaat aaaatcagat actatagaac caaattgaac ttaactcaag
    44521 atcaacttgg agaaaaactc aacactaaaa aggctactat ttcaaattat gagacagggt
    44581 acagaactcc taaacaagat gatttgtttg aaattgctca tattttaaat atcagtatcg
    44641 atgatttgtt tcctacaaga aataataaaa aaaacgacat cacttccata tacagtaaac
    44701 tcacgcctcc aagacaaaaa aacgtactta actacgcaaa tgagcaatta gatgaacaga
    44761 ataaagtcac ttctatagat gaatataaag agtctaaact agtatcgtat attgcatgtg
    44821 gtgcaactgg tgctggcata ggagaagaat tatatgatga catattgcat gaagaagtat
    44881 tttttaaaga agacgaaacg ccatcaaatg ctgatttttg tattttagtt aatggtgatt
    44941 caatggaacc tatgttaaaa caaggaacat acgcttttat taagaaagaa gattctatta
    45001 aagatggtac aattgcactc gttgtattag atggagtaag tcttatcaag cgtgtagata
    45061 tatgcgaaga ctatattaat ttggtatctc taaatccgaa gtatgatgat atcaaagtcg
    45121 cttcgtttag taatattaaa gtaatgggca aagttgtatt gtgattaata gcgcctatat
    45181 ggcactttaa tataaaagac gtctatttca tcagtgttta aaaggagttt ataatgaaaa
    45241 taactaattg caaaataaaa aaagaaacta tagtatatga agttttaact agtggtaatc
    45301 aaccattcac ttatgagtta cctaaagatt tatcgtcaca taatgcgcgt aaatacttgg
    45361 aatttatttc acaaaaatta gatggcgata agttaaatta attcaaagaa taaagtaact
    45421 tcataaagag tacgaagaaa acgatctaat gaccgaactt attcttgaat atttagtaaa
    45481 aaagtatgtt gaagaagaat ataagaaata aacgcctata tggcgtgagg aggatgaggg
    45541 atggaagaga ataaaacttt aaaagaatac ttgcgtaaat ttttagaagg ctacaaatat
    45601 gtagttgaaa acagatacaa ttatcagttt agtagcaatc cagaagcttt cccattcatg
    45661 agaaaagacg attacaagat ttcgatattt tatctaaatc aatctttttt tgaagaacct
    45721 tgcatcgtcg ttatctcaaa tgacagtaaa ttaaaagaaa tatataattt tcgtaatatt
    45781 gatatcaaat atttgtctaa acactttact tcatacatat atgattctaa aaagtatgta
    45841 gaagaacaat ccggattat
//
LOCUS       DAMQYT010000003        45430 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMQYT010000003 DAMQYT010000000
VERSION     DAMQYT010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 45430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45430
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.003"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            145..336
                     /locus_tag="PGA33_000134"
     CDS             145..336
                     /locus_tag="PGA33_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471976.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
     gene            complement(475..660)
                     /locus_tag="PGA33_000135"
     CDS             complement(475..660)
                     /locus_tag="PGA33_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471977.1"
                     /translation="MLYILITALNVILNKLKMKDSNIISFIYSRHDRILYVNLVGNRF
                     KNNISMTKATSNIGRGF"
     gene            complement(596..877)
                     /locus_tag="PGA33_000136"
     CDS             complement(596..877)
                     /locus_tag="PGA33_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDG6471978.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            1166..1861
                     /locus_tag="PGA33_000137"
     CDS             1166..1861
                     /locus_tag="PGA33_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDG6471979.1"
                     /translation="MTVLTDKVAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKEKLQ
                     NVANQLSQDSVKVVPTDVTNKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
     gene            2112..2534
                     /locus_tag="PGA33_000138"
     CDS             2112..2534
                     /locus_tag="PGA33_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDG6471980.1"
                     /translation="MNKKHVFIIIGVILCICIVASVIYLKVKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKEPNTIKTVHFTSLKRGPMGDAVIEGYINENKEDDFVAYGSPEHNYQ
                     FGGSLIKSKNLSTLLKPVHQTKSPDEIKKELESKKNDR"
     gene            2801..3220
                     /locus_tag="PGA33_000139"
     CDS             2801..3220
                     /locus_tag="PGA33_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDG6471981.1"
                     /translation="MSKKKILILTSIMLIILISVAGIYLKVKYDEKEKQKAIYYKEQQ
                     ARITLYLKHNTKEPNTIKSVHFTNSETSPMGSAVIEGYINENKKDNFTAYATPEHNYQ
                     FGGAMIESEKLSELLKPANQLKSPDDIKKELNKKKSH"
     gene            3238..3657
                     /locus_tag="PGA33_000140"
     CDS             3238..3657
                     /locus_tag="PGA33_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDG6471982.1"
                     /translation="MSKKHVFIIIGVILCICTVSTVMHFKMKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKETNTIKSVHFTNLETSPMGSAVIEGYINENKEDDFTAYASPEHNYQ
                     FGGAMIKSEGVDKLLKPAHERKSPEKIKEELDKKEGH"
     gene            4109..4531
                     /locus_tag="PGA33_000141"
     CDS             4109..4531
                     /locus_tag="PGA33_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDG6471983.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDRL"
     gene            complement(4643..6181)
                     /locus_tag="PGA33_000142"
     CDS             complement(4643..6181)
                     /locus_tag="PGA33_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDG6471984.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGKYDDSLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            6414..7232
                     /locus_tag="PGA33_000143"
                     /pseudo
     CDS             6414..7232
                     /locus_tag="PGA33_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503368.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            7495..7860
                     /locus_tag="PGA33_000144"
     CDS             7495..7860
                     /locus_tag="PGA33_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471985.1"
                     /translation="MLKEKESFRLLYQAIREIADKIGDNQLETNSVSLLLLDFDFEHE
                     VFDELYLVILKYLNTVSIENISHSELLNLIENTIPEDREINTFVKNKIIIGFANNYFP
                     ELQVLANEIKSDMASSLKQ"
     gene            8727..9548
                     /gene="cntK"
                     /locus_tag="PGA33_000145"
     CDS             8727..9548
                     /gene="cntK"
                     /locus_tag="PGA33_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HDG6471986.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            9559..10377
                     /gene="cntL"
                     /locus_tag="PGA33_000146"
     CDS             9559..10377
                     /gene="cntL"
                     /locus_tag="PGA33_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HDG6471987.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            10370..11671
                     /gene="cntM"
                     /locus_tag="PGA33_000147"
     CDS             10370..11671
                     /gene="cntM"
                     /locus_tag="PGA33_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HDG6471988.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKAKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFNKDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMVILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQHLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            11814..13412
                     /gene="cntA"
                     /locus_tag="PGA33_000148"
     CDS             11814..13412
                     /gene="cntA"
                     /locus_tag="PGA33_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HDG6471989.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            13425..14360
                     /locus_tag="PGA33_000149"
     CDS             13425..14360
                     /locus_tag="PGA33_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6471990.1"
                     /translation="MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            14357..15226
                     /locus_tag="PGA33_000150"
     CDS             14357..15226
                     /locus_tag="PGA33_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6471991.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            15223..16038
                     /locus_tag="PGA33_000151"
     CDS             15223..16038
                     /locus_tag="PGA33_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6471992.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNPERLGVTGEIIFDGTSMLSLSESQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEQGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            16031..16780
                     /locus_tag="PGA33_000152"
     CDS             16031..16780
                     /locus_tag="PGA33_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6471993.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKEVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            16792..17985
                     /locus_tag="PGA33_000153"
     CDS             16792..17985
                     /locus_tag="PGA33_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDG6471994.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPR
                     PASSL"
     gene            complement(18209..18958)
                     /locus_tag="PGA33_000154"
     CDS             complement(18209..18958)
                     /locus_tag="PGA33_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6471995.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRLMHEDAFIPMIRSQIVASA
                     KYKWHILISFTLSVVLFLLLNHFNIFALNVDSFTVFDIYPLIKGYGSISLITIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSIILETLFYYPYINTWWEFIPALILAG
                     SATYLYLKSYRNFMVSYFYQLAVILVLHIFM"
     gene            19153..20730
                     /locus_tag="PGA33_000155"
     CDS             19153..20730
                     /locus_tag="PGA33_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HDG6471996.1"
                     /translation="MTFLTVMQFIVNIIVVGFMLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            complement(20898..21353)
                     /locus_tag="PGA33_000156"
     CDS             complement(20898..21353)
                     /locus_tag="PGA33_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HDG6471997.1"
                     /translation="MKKLCSLIVVALVCIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            21712..21969
                     /locus_tag="PGA33_000157"
     CDS             21712..21969
                     /locus_tag="PGA33_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDG6471998.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            21969..22235
                     /locus_tag="PGA33_000158"
     CDS             21969..22235
                     /locus_tag="PGA33_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDG6471999.1"
                     /translation="MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
     gene            22590..23666
                     /locus_tag="PGA33_000159"
     CDS             22590..23666
                     /locus_tag="PGA33_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDG6472000.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAIEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
     gene            23914..24576
                     /locus_tag="PGA33_000160"
     CDS             23914..24576
                     /locus_tag="PGA33_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDG6472001.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLFSPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDRKRAKQLIKDVGLGHYQLSSEVENMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDVNNKEKIENIIFKLADQGVAIMWITHSDDQSMRHFQKRITIVDGQISNTEELNQ
                     HE"
     gene            24569..25345
                     /gene="fetB"
                     /locus_tag="PGA33_000161"
     CDS             24569..25345
                     /gene="fetB"
                     /locus_tag="PGA33_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDG6472002.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            25741..26928
                     /locus_tag="PGA33_000162"
     CDS             25741..26928
                     /locus_tag="PGA33_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDG6472003.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(26990..28342)
                     /locus_tag="PGA33_000163"
     CDS             complement(26990..28342)
                     /locus_tag="PGA33_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HDG6472004.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLESARNKAQHFQKMMLDYLDTDDVTSLSTNDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(28950..30587)
                     /locus_tag="PGA33_000164"
     CDS             complement(28950..30587)
                     /locus_tag="PGA33_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDG6472005.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIIVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
     gene            30767..31366
                     /locus_tag="PGA33_000165"
     CDS             30767..31366
                     /locus_tag="PGA33_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HDG6472006.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARTEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            31370..31510
                     /locus_tag="PGA33_000166"
     CDS             31370..31510
                     /locus_tag="PGA33_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472007.1"
                     /translation="MSKAYRLYNNIQDDTKQPMAHAVGCFFRCVCHGQHFDVGIPLQA
                     WE"
     gene            31590..31709
                     /locus_tag="PGA33_000167"
     CDS             31590..31709
                     /locus_tag="PGA33_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472008.1"
                     /translation="MGGCPSVTMDACALLQKFDFCNNISHFRHFFAIKQPIER"
     gene            32036..33262
                     /locus_tag="PGA33_000168"
     CDS             32036..33262
                     /locus_tag="PGA33_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6472009.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAEISGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDAIFVVDSNNHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDQQRLVGLITRANVVDIVYDTIWGDSEDTVQTEHVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            33259..33894
                     /locus_tag="PGA33_000169"
     CDS             33259..33894
                     /locus_tag="PGA33_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6472010.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIVLTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            33911..34852
                     /locus_tag="PGA33_000170"
     CDS             33911..34852
                     /locus_tag="PGA33_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDG6472011.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKNPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            34852..35547
                     /locus_tag="PGA33_000171"
     CDS             34852..35547
                     /locus_tag="PGA33_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6472012.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            35841..37241
                     /locus_tag="PGA33_000172"
     CDS             35841..37241
                     /locus_tag="PGA33_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDG6472013.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLITVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            37467..38402
                     /locus_tag="PGA33_000173"
     CDS             37467..38402
                     /locus_tag="PGA33_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDG6472014.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            38724..39575
                     /locus_tag="PGA33_000174"
     CDS             38724..39575
                     /locus_tag="PGA33_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDG6472015.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYQTWK
                     DGMNPIK"
     gene            complement(39667..41163)
                     /locus_tag="PGA33_000175"
     CDS             complement(39667..41163)
                     /locus_tag="PGA33_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDG6472016.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDS
                     VTSKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQTNYKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLSLCICIALALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
     gene            41395..43224
                     /locus_tag="PGA33_000176"
     CDS             41395..43224
                     /locus_tag="PGA33_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDG6472017.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSAKNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     LPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(43364..45418)
                     /locus_tag="PGA33_000177"
     CDS             complement(43364..45418)
                     /locus_tag="PGA33_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001806773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDG6472018.1"
                     /translation="MFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIPIQVDFQFN
                     SEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYFIHWIWPAL
                     PMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGIISFKIAVTA
                     LVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNNTLTFIQLL
                     TPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWNTFSYALNG
                     FVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFWYKDFYFPK
                     NIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKGQAFEYRND
                     LLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQHFKNESKK
                     DKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLERLIDKGKA
                     TYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKESKKQEFEN
                     SFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRAQRRKERQE
                     NSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYNEIVLSLQS
                     KH"
BASE COUNT        15451 a         6631 c         8657 g        14691 t
ORIGIN      
        1 aaaatagtga taaatttgtg aaatacatca caaatccctt tatttatttg gaaattcatg
       61 taatattaga cttgtaagaa gttaataaat agagagagac gagagagttt atataaatac
      121 tatataaaca ttggagtgat gattatgaga aaagagattg aagcgcttat tttctcagac
      181 gtaagtagct atgatattta cgtgaacact ggtgtaaatc aaggattaat tggtgacatc
      241 aaagatggtt acctaactat tgattctatg ccttacattg atgctgagcg tttgtatcac
      301 tttgctatgg aacgtaaatc gttagtcact aactagttct tattgccaat gattactacc
      361 cctagtcggc ggcaattgaa gtgtgattga tgtaacttgc cctcgttggt gagcaattga
      421 gggcagaccc ctttaattaa gtaaacccta actccccaca aatctggaac gatactaaaa
      481 gccacgtcct atattggatg tggctttagt catacttata ttatttttaa aacgattacc
      541 tacaagattt acatataaaa ttctatcatg tctgctatat ataaacgaaa tgatattaga
      601 atccttcatt ttcaacttgt ttaaaattac gtttaatgcc gttataagaa tatacaacat
      661 tatataattt tacttgggcg tcatggacac ttgagaaggg cattcttaca tcgtccatat
      721 ttttaacttc gtgtcgaaat gctttttcag ctttttgtct ttctgcaaga gacatactta
      781 gccattcttc tttgctaaat aaatcttcga atcgaaaatc aaatgcggtt cttttacttt
      841 ctgctcttaa tgctaaaaca cgctcatgat atgtcacgtt ttattcctcc ctctaatcat
      901 tcaatcgaat gataaatcac agtattaact ttattactaa tataatgttt ttacaataaa
      961 gaagtgacga tttttcaaca tttttaaaaa taaaaaatat aagcaacgat taattgtatg
     1021 tttcgaatgg cttgtaatgt aatttatcta tgaaaatgaa gcgagttatt atgaccgtta
     1081 tatgaattgt gatacgcttt ttaaaagtaa tatgtattta tgatttaccc tgaaaaaata
     1141 aaaagaatca aaggagtcga tcattatgac agtattaaca gataaagtag cagtagttac
     1201 aggtgcaggt agtggtattg gagaagcaat tgcaacatta ctacatgaag aaggggcaaa
     1261 agttgtctta gcaggtagaa ataaagaaaa attacaaaac gtagcgaatc aattgtcaca
     1321 agatagtgtg aaggtagtgc caacagatgt aacgaataaa gaagaagtcg atgaattgat
     1381 aaaaattgca caacaaacat tcggtggttt ggatattgtt atcaatagtg cggggcaaat
     1441 gttgtcgtct aagattactg attatcaagt agatgagtgg gatagtatga ttgatgtgaa
     1501 tatcaaaggc actttatata cggcacaggc tgcattacca actatgttag aacaatcaag
     1561 tggccatctt attaacattg catctatttc tggctttgaa gtaacgaaaa gtagtacgat
     1621 ttatagtgcg acgaaagcag cagttcacac tattactcaa ggattagaaa aagagttggc
     1681 aaagacaggc gttaaagtaa caagcatttc tccaggaatg gtagatacag ccataactgc
     1741 cgcatacaat ccaacagatc gtaaaaaact tgaaccacaa gatattgcag aagcagtatt
     1801 atatgcatta acacaaccaa agcacgttaa cgtgaatgaa attacagtgc gtcctgtata
     1861 aaaaggtgtt tgaggtctgg ggtataggca tttaatgtaa tagctatcga ttttgttaat
     1921 gttagtaagg caataattac atgagggcat aataggcaat tattccttgt acaattctca
     1981 gtaattgctg attttaacaa tcaagttaat gaaggcgacg atgttagtgt tatggtttat
     2041 aatctgtaat gttcataatt atagattata aaaatttttt cattttccac cacattttag
     2101 gagttacttt aatgaacaaa aaacatgttt ttataattat tggtgtcatt ttatgtatat
     2161 gtatagttgc atcagtcatt tatttaaaag tgaaatatga cgaaaaagaa aaacaaaaag
     2221 caatatacta taaagaacaa caggagcgta ttacactcta tcttaagcat aatactaaag
     2281 aacccaatac catcaaaact gtacatttca caagtttaaa aagaggacct atgggcgatg
     2341 cagtaattga aggttacata aatgaaaata aagaagatga ttttgttgca tatgggtctc
     2401 cagaacataa ttatcaattt ggtggaagtt taatcaaaag taaaaattta agcacgttat
     2461 taaaaccagt acatcaaacc aaatcacctg atgagataaa aaaggaatta gagtctaaaa
     2521 agaacgaccg ctaaaactat gcggccgttt taattttgtt ttcaagttaa tcatacttac
     2581 atataaaaat gaaaagttga atttttagta caatttccac tgtatttatc tgttaaccac
     2641 tttatacccg caatatttcg ttgatcttat ataaacactt ttggctgctt ttccttgacc
     2701 tagattaaaa ttatagattt tctttgtaag agtgtgtttt gtagtatcat aaatatgtaa
     2761 actgtataaa attccaccac attttaggag ctggtgatag atgtcgaaaa agaaaatttt
     2821 aatattaact agtattatgt taatcatatt aattagtgtt gcaggtatct atttaaaagt
     2881 gaaatatgac gaaaaagaaa aacaaaaagc aatatactat aaagaacaac aagcgcgcat
     2941 tacactttat ctcaagcata acactaaaga accgaataca atcaaatctg tacatttcac
     3001 aaactcggaa acaagtccta tgggaagtgc tgtgattgaa ggttacataa atgaaaacaa
     3061 aaaagataat tttactgctt atgctacacc agaacataat tatcaatttg gtggtgctat
     3121 gatagaaagt gaaaaattaa gcgagttact aaagccagcc aatcagttaa aatcaccaga
     3181 tgatataaaa aaagaactaa ataaaaagaa gagtcactaa agttaggagt tactttaatg
     3241 tccaaaaaac atgtttttat aattattggt gtcatattgt gtatatgtac agtttctacg
     3301 gtcatgcatt ttaaaatgaa atatgatgaa aaagaaaaac aaaaagcgat ttactacaaa
     3361 gaacaacaag aacgtattac actctatctt aagcataata ctaaagaaac gaacacgatt
     3421 aaatctgtac atttcacaaa cttggaaaca agtcctatgg gaagtgctgt gattgaagga
     3481 tacatcaatg aaaataaaga agatgatttt actgcttatg catcgcctga acataattat
     3541 caatttggtg gcgctatgat aaaaagtgaa ggagtagata aattattaaa accagcacat
     3601 gaaagaaaat caccagaaaa aatcaaagaa gaattagata aaaaagaagg ccactagggt
     3661 cttctttatt tttgatttaa tcttccaata atctatgtca ttgctatcga aggtgtttcg
     3721 caattaatat aaatcacttc atcatcacca atacttcccc agttttgtac agtacattaa
     3781 cacaaacgaa ccacgttaat ttaaatggaa taatagtttg gccattataa gaacaatata
     3841 tatcgaataa caatgtcgat gacgtgattt attgacatta tcaaattttc tcaaatgtgt
     3901 gtgatttttt ataaaaaagg gtagagcaag gtataatatc ctttcaatct gttttcatat
     3961 tttatatatt ttttaaatat aagtgctaaa tgttttaact aaagcataga ttgacaagat
     4021 gttatacaga atttcaaatt ctatccaata ttgttcgaag tgtagtatca ctggattggt
     4081 attaaacaat gtaaaggaga gattgcaaat gccgtataat tacaagaaac aaaatggaga
     4141 gttaatgtct gtaatgagcc aaggtgaaaa gtttattcat caatcacccg ttaatgatga
     4201 acttagtgca ttgattaagt tattaatttc taaaattaac ggttgtcatt attgtgttga
     4261 tatccataaa aaagaattaa aggaattggg tgtaacacaa atgaaaattg atgaagtctt
     4321 gagttttaga catttagatt tatttactga tcaagaaaaa gtgacgcttg aatttgcaga
     4381 aatgttaaat tcaatcaaag actttaagaa gtttgaaatt attgaccggc taaaatcatt
     4441 ttatgatgaa gaacaaatta ttgatcttgt ctttgttgta aaccaaatta acggttggaa
     4501 cagattaaat attattagtg atagactata attgttcata taaatgcaga gtttcatctc
     4561 gaacgctata tcataacaaa tcatgccact atacaggtca aatcttgtat agtggcattt
     4621 taatttatcc ctttgaatac tgttatttaa cgaatatcgg tccacctggt ccaacaggga
     4681 tacctaatag gaaccaaatg atgacaaata ctgtccatac aatacttagc gcgattgaat
     4741 acggcattaa actagaaagt aaggctccga gtttcatgcg tttatcgtat ttttgtgcat
     4801 aagttaataa taaaggtaag tacggcatca tcggtgtaat tggattggta attgaatcgc
     4861 ctacacggta aatgacttgt gtgaatgcgg gatgaaagcc gataaggatt aacattggta
     4921 cgaatatcgg tcctaaaata ccccatttag ccgatgcgct tccgattaac atgttgacca
     4981 ttgcactcag tacaataata cctagtatca atacaatacc gttttgatgt tctaataatt
     5041 tggcaccttt aacagcagcg ataattccta aattactcca ctttaaatac gcaagtagct
     5101 gtgctgcaaa aaacacaata acgataaatg ttcccattga tcctacagca tcgccgaaca
     5161 ttttacctaa gtcttttgta tttttaattt ctttgcttaa aatcccataa actaatccag
     5221 gtactaaaaa tacgacaaga ataattaatc cgacaccgtt aattaatggc gcatcgtcta
     5281 gtaagctgcc tgttttagca tttcttaaaa agctatgttc aggaatggct gtaataatta
     5341 ataaaataat tgtgactatg aaactgatat ttgcccattt taaagcatgt gcttctttgt
     5401 cagtaatatg tgaagatgtt tcttcatggt catcatgcat taaactgtca tcatattttc
     5461 ctaatctagg aataatgagc ttagttgtaa ctagcaatat cgtaggaagt aatacaacga
     5521 cactcgctgc gataaagtac cagttcatag caacgtttgt tttaatagaa tctgaaacga
     5581 tacgtgttgc cggttctgta aatgaataga ccaaagcatc ttgcatacca acaactatat
     5641 ttgctgcaaa tcctccaaca gcggaagcat atgccatcgt tagtccagcg atagggtgat
     5701 agccaatttt aataaaaagc attgctgcaa gcggcggcaa gataattgtc gcagcatcgc
     5761 cggctgtact acctaaaata ccaattaata taatagtcgg taaaattaag aagcgtggtg
     5821 cgcgattcac aacagaaatc attaacttat cgaagtatcc tgttttctct gcaacaccaa
     5881 taccaatcat cactgctagt actaagccta atgctgggaa ctctgagaaa tttttaatcg
     5941 tatcattcat tatcatcgta aatccatcat ggctaattat atttttaata taaatggttt
     6001 gatgcgtacc tggatgctta acagatacat taaataatga gataacccat gtcataatgg
     6061 ctaagcccac acacattaaa aagaataaga cgctaggatc tggcaattta tttccgattt
     6121 tttcaacact attcaagaaa cgattgacga tagacccctt ttgttgatgt tttgatgtca
     6181 tcaattattc ccccctttgt taaatattta aagtgtaaca aaaaatactc tcaaaagtaa
     6241 caattttcag aaaataaaaa aactaatatt gttaaatatt ttgagttatt caatagaaag
     6301 tgtatagcag agtagttaag actgcctgaa gacttatcta ttaggtttat gaagcatcga
     6361 acagtggaaa ataaggactg taagtttaag atatgttgta taggagtgac tgaatgaaac
     6421 gtttggaaaa taaagtagca gtcgtaacag gagcaagtac aggtatcggt caagcttctg
     6481 caatcgcttt agctcaataa ggtgcgtatg tattggcggt agacatagct gaagcggtat
     6541 cggagactgt cgataaaatt aaaagtaatg gtgacaatgc gaaggcgtat aatgtggata
     6601 atgcaagcga acaacaagtg gtagactttg tgtctgacat aaaggaacag tttggaagaa
     6661 tcgatgtgtt gtttaataat gccggtgtgg ataatgcggc tgatagaatt catgagtatc
     6721 caatagatgt gtatgacaag attatgaatg tagatatgcg tgggacattt ttaatgacga
     6781 aaatgatgtt acctttaatg atgaatcaag gtggctctat tgttaatacg tcttcatttt
     6841 ccggacaagc agcagacttg tatcgctctg gatataatgc tgcgaaaggt gcagtgatta
     6901 attttacaaa atcaatcgca attgagtatg gccgtgatag cattcgagcc aatgcgattg
     6961 caccaggtac aattgaaacc ccgttagtag ataaactgac aggtacgagt gaggatgatg
     7021 caggtaaaac atttagagaa aatcaaaaat ggatgactcc gctgggacgt ttaggtaaac
     7081 cagaagaagt tgctaaatta gtagtcttct tagcatctga cgacagttca ttcataactg
     7141 gagagacgat tcgaattgat ggtggtgtga tggcttacac atggcctggc gagatgttaa
     7201 gtgatgattc gtggaagcgg acattggaat aattatttaa aatcgatatc cagtggaaaa
     7261 gaatttggca tgaagtaggc aatgtatgca tattgtcgca atgaaagaca tttaaagcgg
     7321 agattaatta atctttgaaa atcacatatt gttcatttga agtgtattgc ttaaagcttg
     7381 ttttaacgta ttgtaggtcg tgcatctaat ttatcagaat ctcgagttca aacttttgga
     7441 gtatctttaa aataagctgt attttatcta ttctaataaa ttaaggagaa ttttatgtta
     7501 aaagaaaaag aaagttttag attgctatat caagctataa gagagattgc agataaaatt
     7561 ggagataatc agttagaaac taattccgtt agtttattat tattggactt tgattttgaa
     7621 catgaagtat ttgatgaatt gtatcttgtg attttaaaat atttaaatac agtaagtata
     7681 gagaacataa gtcatagtga gcttttgaat ttaatagaaa atacgattcc agaagatcga
     7741 gagattaaca cgtttgtaaa aaataaaata ataattggct ttgctaataa ttacttccct
     7801 gaattacaag tattagcaaa cgaaataaaa tctgatatgg ctagttcatt aaaacaatga
     7861 tatttttatt taaattttta aagctttgta cgaaattgta caaagctttt ttggtgcgta
     7921 ttgtatgggc aacaacttga cgatgaaaat ccgttacagg attggtaata ggaaatgtta
     7981 gcgaaagaca agggtatcca ttgtagatta acaaaaggac gtttccacaa gtgtgggtta
     8041 ttctcactaa agcaatacgc agagacaact tacgtaaaat tttgaactga ctagaacgga
     8101 acttctactc aattattgat aaaaattttc aaaaagactt gaatgtgctg agaatacgaa
     8161 gtttatggaa ggattatcaa aatataaatg tgcattcatt tacaaccttt attgacaatg
     8221 attctcaact aatatagtat ataatcaaat cgtaatagtt acgatttgtt ttctgcaact
     8281 tttttgaagt tttagttgag gtgaaaacag taaaagcatc taagtgaatg tagttaacgg
     8341 acaactgcat tcgcttgtag agccacaaga agcaacttta aataaggttt acggttgcat
     8401 tttgatacaa caaccgatta ctaagtcatg ctttccactt tgcgggttag catgacttac
     8461 ctaatagata gagctattag gttcagcttc taaaaaatta cagttttaga ggaatacagt
     8521 tgcttgcttc gcaacaactg cataagagcc atggttttcg cttttgcgaa ttagcatgac
     8581 ttacctacta gatagagcta ttaggttcat cttctaaaaa attacaggtt tagaggaata
     8641 cagttgtttg cttcgcaaca actgcataag agcctctagt aattaaaatt acagaggctc
     8701 taaaaataca tctaaaggag tgtcgtatga atcggcaggt tatagaattt tctaagtata
     8761 atccttcggg gaatatgacg atacttgttc attcaaaaca tgatgctagt gaatatgcat
     8821 ctatcgccaa tcagttgatg gccgcaacac atgtatgctg tgaacaggta ggctttatag
     8881 aatcaacaca aaatgatgat ggtaatgatt ttcacttagt tatgagcggt aatgaatttt
     8941 gcggtaatgc gacgatgtca tatatacatc atttgcagga aagtcatttg cttaaagacc
     9001 aacagtttaa ggtgaaggtg tctggctgtt cggatttagt gcaatgcgca attcatgatt
     9061 gccaatacta tgaagttcaa atgccacaag cccatcgtgt tgtgccaaca acaattaata
     9121 tgggtaatca ttcatggaaa gcaatagaaa ttatttatga aacatatgta cattatgtga
     9181 ttccagttaa acaagtaaca acagaaattc aacatttggt tgaagcgttt gtgcgtgagc
     9241 aacaatggag tcacaaatat aaaacagtag gtatgatgct ttttgatgaa caacgtcaat
     9301 ttttacagcc attaatctat ataccagaaa ttcaaagttt aatttgggaa aatagctgtg
     9361 gttctggtac agcatcaatt ggggttttta ataattatca acgtaatgac gcatgcaaag
     9421 attttacagt acatcagcca gggggcagta ttttagtgac atcaaagcga tgtcatcaat
     9481 tgggatatca aacttcaatt aaaggacagg ttacaactgt agctacagga aaagcatata
     9541 tagaataagg agcctacaat gaataacttt aataatgaaa tcaaattgat attacaacaa
     9601 tatttagaaa agtttgaagc gcattacgag cgtgtattac aagacgatca atatatcgaa
     9661 gcattagaaa cattgatgga tgactatagt gaatttattt taaatcctat ttatgaacaa
     9721 caatttaatg cttggcgtga cgttgaagaa aaagcacaat taataaaatc actgcaatat
     9781 attacagcgc agtgtgttaa acaagtggaa gtcattagag cgagacgtct attagacgga
     9841 caggcgtcta ccacaggtta ctttgacaat atagaacatt gtattgatga agagtttgga
     9901 caatgtagta tagctagcaa tgacaaatta ttgttagttg gttcaggtgc atatccaatg
     9961 acgttaattc aagtagcaaa agaaacaggt gcttcagtta tcggtattga tattgatcca
    10021 caagccgttg acctagggcg cagaatcgtt aacgtcttag caccaaatga agatataaca
    10081 attacggatc aaaaggtatc tgaacttaaa gatatcaaag atgtgacgca tatcatattc
    10141 agctcgacaa ttcctttaaa gtacagcatt ttagaagaat tatatgattt aacaaatgaa
    10201 aatgtcgtag ttgcaatgcg ctttggtgat ggcatcaaag caatatttaa ttatccgtca
    10261 caagaaacag cggaagataa gtggcaatgt gtgaataaac atatgagacc acagcaaatt
    10321 tttgatatag cactttataa aaaagcagct ataaaggtag gtattacgga tgtctaaatt
    10381 attaatgata ggcactggtc cggtcgcaat ccaattagcg aatatttgct atttaaaatc
    10441 agattatgag attgatatgg ttggacgtgc ctcaacatca gaaaaatcaa aacgcttata
    10501 tcaagcgtat aaaaaagcga aacaatttga agtcaaaata caaaacgagg cgcatcaaca
    10561 tctggaaggt aagtttgaaa ttaatcgttt gtataaagat gttaaaaacg ttaagggtga
    10621 atacgaaacg gttgtcatgg catgcacagc agatgcttat tatgacacac tacagcaatt
    10681 gtcgttagaa actttgcaaa gtgtcaaaca tgtcatttta atatcaccga catttggttc
    10741 gcaaatgatt gtcgaacaat ttatgtctaa atttaataaa gatatcgaag tgatttcatt
    10801 ctcaacttat cttggcgata cacgtattgt tgataaagaa gcgcctaatc atgtgttgac
    10861 aacaggtgta aaaaagaaat tgtacatggg atcgacacat tcaaactcaa caatgtgtca
    10921 acgaatctct gctttagctg agcaattgaa gattcaatta gaagtcgttg aatcgccact
    10981 gcatgctgaa acgcgaaaca gttcgcttta tgtgcaccca ccactattta tgaatgactt
    11041 ttcattgaaa gccattttcg aaggaacaga tgtaccggtt tatgtgtata agttatttcc
    11101 tgaaggaccg ataacgatga cactaatccg tgaaatgcgt ttaatgtgga aggaaatgat
    11161 ggttatttta caagcattta gagtgccgtc agtcaacctg cttcaattta tggtgaagga
    11221 aaattatcca gtacgtcctg aaactttgga tgaaggtgat attgagcatt tcgaaatctt
    11281 gccagatatc ttacaagaat atctgcttta tgtaagatat accgcaatcc tcattgatcc
    11341 attttcacag ccagacgaaa acggacatta ctttgatttt tcagctgtac catttaagca
    11401 agtctataaa aatgaacagg atgttgttca aattccaaga atgccaagtg aagattatta
    11461 cagaacggcg atgattcagc atattgggaa aatgctaggt atcaaaacgc caatgattga
    11521 tcagttccta actcgctatg aagcaagttg ccaggcgtac aaggatatgc atcaagatca
    11581 acacttatct tctcaattta atacaaatct atttgaagga gataaagcac tcgtcacaaa
    11641 atttttggaa atcaatagaa cgctttcata ataagggttt gaagttttat aatagaaaaa
    11701 aattattgaa ttatgtttga catttacata aaaataagca aataattgag aaaaataatc
    11761 attacgattt gattaagtaa tgcaacttat caatttagaa agaggaaaag caaatgagaa
    11821 aactaactaa aatgagtgca atgttacttg catcagggct aattttaact ggttgtggcg
    11881 gtaataaagg tttagaggag aaaaaagaaa acaagcaatt aacgtatacg acggttaaag
    11941 atatcggtga tatgaatccg catgtttacg gtggatcaat gtctgctgaa agtatgatat
    12001 acgagccgct tgtacgtaac acgaaagatg gtattaagcc tttactagct aaaaagtggg
    12061 atgtgtctga agatgggaag acatacacgt tccatttgag agatgacgtt aaattccatg
    12121 atggtacgcc atttgatgct gacgcagtta agaaaaatat tgacgcagtt caagaaaaca
    12181 aaaaattgca ttcttggtta aagatttcga cattaattga caatgttaaa gttaaagata
    12241 agtacacggt tgaattgaat ttgaaagaag catatcaacc tgcattggct gaattagcga
    12301 tgcctcgtcc atatgtattt gtgtctccaa aagactttaa aaacggtaca acaaaagatg
    12361 gcgttaaaaa gttcgatggt actggtccat ttaaattagg tgaacacaaa aaagatgagt
    12421 ctgcagactt taacaaaaat gatcaatact ggggcgaaaa gtctaaactt aacaaagtac
    12481 aagcaaaagt aatgcctgct ggtgaaacag cattcctatc aatgaaaaaa ggtgaaacga
    12541 actttgcctt cacagatgat agaggtacag atagcttaga caaagactct ttaaaacaat
    12601 tgaaagatac aggtgactat caagttaagc gtagtcaacc tatgaatacg aaaatgttag
    12661 ttgtcaattc tggtaaaaaa gataacgctg tgagtgacaa aacagtcaga caagcgattg
    12721 gtcatatggt aaacagagat aaaattgcca aagaaatttt agatggtcaa gaaaaaccag
    12781 caactcaatt atttgcgaaa aatgtaacag acattaattt cgatatgcca acacgtaagt
    12841 atgaccttaa aaaagcagaa tcattattag atgaagctgg ttggaagaaa ggtaaagaca
    12901 gcgatgttcg tcaaaaagat ggtaaaaacc ttgaaatggc aatgtactat gacaaaggtt
    12961 cttcaagtca aaaagaacaa gcagaatact tacaagcaga atttaagaaa atgggtatta
    13021 agttaaacat caatggcgaa acatcagata aaattgctga acgtcgtact tctggtgatt
    13081 atgacttaat gttcaaccaa acttggggat tattgtacga tccacaaagt actattgcag
    13141 catttaaagc gaaaaatggt tatgaaagtg caacatcagg cattgagaac aaagataaaa
    13201 tatacaacag cattgatgac gcatttaaaa tccaaaacgg taaagagcgt tcagacgctt
    13261 ataaaaacat tttgaaacaa attgatgatg aaggtatctt tatccctatt tcacacggta
    13321 gtatgacagt tgttgcacca aaagatttag aaaaagtatc attcacacaa tcacagtatg
    13381 aattaccatt caatgaaatg cagtataaat aaaggagcaa ttagatgttc aaatttatct
    13441 taaaacgtat tgcgctcatg tttccattga tgattgtagt aagttttatg acatttctat
    13501 tgacgtatat tacaaatgaa aatccagctg tgacaatttt acatgcacaa gggacgccaa
    13561 atgtaacacc agagttgatt gcagaaacga atgagaagta cggtttcaat gatccattat
    13621 taattcaata taaaaattgg ttacttgaag cgatgcaatt taattttggt acaagctaca
    13681 ttacaggtga cccagttgct gaacgtattg gtccagcatt tatgaataca ttgaaattaa
    13741 caataatttc aagtgttatg gtgatgatta catcaattat tttaggtgta gttagtgcat
    13801 taaaaagagg aaagttcact gatcgtgcga tacgttcagt ggctttcttt ctaactgcat
    13861 taccatcata ttggatagct tcaatactta ttatttacgt ttcagtgaag ttaaacatat
    13921 tgccgacttc tggattaaca ggtccagaaa gttacatatt gccagtgatc gttattacga
    13981 ttgcctatgc tggtatttac tttagaaatg ttagacgctc gatggtggaa caattaaatg
    14041 aagattatgt actttattta agagcaagcg gtgtgaaatc tatcacatta atgttgcatg
    14101 tgttgcgtaa tgctttacaa gttgcggtat caatcttttg tatgtctata ccaatgataa
    14161 tgggtggact agttgttatc gagtatatct ttgcatggcc tggactaggt caattaagtt
    14221 taaaagcaat acttgaacac gattttccag tcattcaagc atatgtatta attgtagcgg
    14281 tattatttat tgtatttaat acattagcag atatcattaa tgcgctatta aatccaagat
    14341 taagggaggg cgcacgatga taattttaaa acgattatta caagataaag gtgcagtaat
    14401 tgctttaggc attattgtat tatatgtctt tttaggatta gcagcaccac ttgtgacatt
    14461 ttatgatcct aaccatatcg atacagcaaa caaatttgct ggcatgagtt ttcaacatct
    14521 actaggtact gaccatttag gtagagatat tttaactagg ttaatttatg cgattagacc
    14581 aagtttgtta tatgtctttg ttgcgctatt tgtttctgta cttattggat ctattttagg
    14641 attcttatca ggatatttcc aagggtttgt tgacgcctta atcatgcgtg cgtgtgatgt
    14701 tatgttggca ttcccaagtt atgttgtaac gttagcatta attgcattgt ttggaatggg
    14761 tgccgaaaat attatcatgg catttatttt gacgcgttgg gcatggttct gtcgtgttat
    14821 acgtacaagt gttatgcagt acactgcttc tgaccatgta agatttgcta aaacaatcgg
    14881 tatgaatgat atgaaaatta ttcacaaaca tattatgcca ttaacattag cagatattgc
    14941 tatcatctct agtagctcga tgtgttcaat gatcttgcaa atatctggct tttcattttt
    15001 aggattaggt gtcaaagcgc ctactgcaga gtggggcatg atgcttaacg aagctagaaa
    15061 agtgatgttt acacatcctg aaatgatgtt tgcgccaggt attgccatag tgattatagt
    15121 gatggcattt aacttcttat ccgatgcttt acaaattgct attgatcccc gcatctcttc
    15181 taaagataaa cttcgttctg tgaaaaaagg agtggtgcaa tcatgacatt gttaacagtt
    15241 aaacatttga cgattacaga tacctggaca gatcaaccac tcgtgagtga tgtgaatttt
    15301 acattaacta agggtgaaac tttaggcgtt attggagaaa gtggtagtgg taaatcaatc
    15361 acttgtaaat cgattattgg tttgaatccc gaacgactcg gggtgacagg tgaaattatc
    15421 tttgatggta catcaatgtt gtcattatct gaatcgcaat tgaaaaagta ccgtggtaaa
    15481 gacattgcga tggtcatgca acaaggtagt cgtgcctttg acccatcaac tactgtcggt
    15541 aaacaaatgt ttgagactat gaaagtacat acgtcaatgt ctacacaaga aattgaaaag
    15601 acattgattg aatatatgga ttatttaagt ttgaaagatc ctaaacgtat attaaaatca
    15661 tacccttaca tgttatcagg aggaatgtta cagcgattga tgattgcttt agcgttagct
    15721 ttgaaaccaa agttaatcat tgctgatgag ccgacaacgg ctttagatac aattacacaa
    15781 tatgatgtac tggaagcatt tatagatatt aaaaaacact ttgactgtgc gatgattttc
    15841 atttcacatg atttaacggt tattaacaag attgcagacc gtgttgttgt gatgaaaaat
    15901 ggtcagctta ttgaacaagg gacacgtgaa tcagtcttgc atcatccaga acatgtttat
    15961 acgaagtatt tattatcaac gaagaagaag attaatgatc attttaaaca tgtgatgagg
    16021 ggtgatgtac atgattaaaa ttaaagatgt tgaaaagtca tatcaaagcg cacatgtttt
    16081 taagcgtcgt cgaacaccta tcgtgaaagg tgtgtcattt gagtgtccaa tcggtgcgac
    16141 gattgcgatt atcggagaaa gtggtagcgg taaatcgacg ttgagtcgta tgatattagg
    16201 tattgagaaa ccggataaag gttgtgtaac cttaaatgat caaccgatgc ataagaagaa
    16261 agtgagacgt catcaaattg gtgctgtatt tcaagattat acgtcatcat tacatccatt
    16321 tcagactgtt agagaaatct tatttgaagt gatgtgtcaa tgtgatggac aacctaaaga
    16381 agttatggaa gtccaagcaa ttacattgtt ggaagaagtc ggtctatcta aggcatacat
    16441 ggataaatat cctaatatgt tatcaggtgg agaggcgcaa cgtgttgcga ttgcgcgtgc
    16501 aatatgtatt aaccctaaat atattttgtt tgatgaagcc attagttcac tcgacatgtc
    16561 aattcaaaca caaatattag atttattgat tcatttacgt gaaacgcgtc agttgagtta
    16621 tatttttatc acacatgata ttcaagctgc cacgtattta tgtgatcaat taattatttt
    16681 taaaaacgga aaaatagaag aacaaattcc gacaagcgca ttgcataaaa gtgacaatgc
    16741 ttatacaaga gaattaatag aaaaacaact atcattctaa ggagtgagat aatgaaaggt
    16801 gcaatggctt ggcccttttt gagattatat atattaacat tgatgttctt tagtgccaat
    16861 gcaatcttaa acgtgtttat acctttacga gggcatgatt taggcgcaac gaatacggtt
    16921 atcggtatcg ttatgggggc atacatgtta acagcaatgg tatttcgacc atgggcagga
    16981 caaattattg ctcgtgtcgg tcccattaaa gtattaagaa ttattttgat tatcaatgcc
    17041 atagctttaa ttatttatgg ttttactggc ttagaaggtt atttcgtagc acgtgttatg
    17101 caaggtgtgt gtacggcatt cttttctatg tctttacagc taggtattat tgatgcatta
    17161 ccagaggaac atcgttctga aggtgtatca ttgtactcgc tattttcaac gattccaaac
    17221 ttaatcggac cattagttgc cgtaggtatt tggaatgcaa ataatatttc actatttgca
    17281 attgtcatta tctttatcgc attaacaaca acattctttg gctatcgcgt gacctttgct
    17341 gaacaggaac ccgatacgtc agataagatt gaaaaaatgc cgtttaacgc tgtaactgtt
    17401 tttgcgcaat ttttcaaaaa taaagagttg ttgaacagtg gtattatcat gattgttgca
    17461 tcgattgtat ttggtgcagt tagtacattt gtaccgttat acacagtgag tttaggattc
    17521 gcgaatgcgg gaatcttttt gacaatacag gccatcgcag ttgttgcggc aagattttac
    17581 ttaaggaaat acattccgtc agatggtatg tggcatccta aatatatggt atctgtacta
    17641 tcattattag taatcgcgtc atttgtagtg gcatttggtc cgcaagtagg tgcaattatt
    17701 ttctatggta gtgcgatatt aataggaatg acgcaagcaa tggtgtaccc aacattaaca
    17761 tcatacttaa gcttcgtctt accaaaagta ggtcgtaata tgttgttagg tttatttatt
    17821 gcctgtgcag acttaggtat atcgttaggt ggcgcattga tgggacctat ttccgattta
    17881 gtaggattta aatggatgta tctaatttgt ggtatgttag tcattgtaat aatgattatg
    17941 agtttcttga aaaagccaac accacgtcca gcgagtagtc tttaatgaag tgaattaaag
    18001 catattaagt taatgaatat ttaaatttta aaaggtatat tgagcatggc gattcatgtg
    18061 cttcatgcta ggacatgaaa cattctatat ggctcgtttt tagaacgaca gtatatctaa
    18121 ataaagcacg cttaaaagtg agttttgata ctgactttta agtgtgcttt ttgatgtgta
    18181 tggataatgg ataatactct gaaaaatttt acatgaaaat atgcaaaacg agtataactg
    18241 ctaattgata gaaatagctc accataaaat tacggtatga ttttaaatat aagtaagtcg
    18301 cactacctgc tagtatcaat gctggaatga attcccacca tgtattaatg tatggatagt
    18361 agaacagagt ttcaaggata atggacaata ctattgtaat ctttaaaggt attaatctgc
    18421 ttaattcttg aattaaaata tgacggaaaa taagttgaca aatcaaagta tttaatataa
    18481 tggttaacga aaatatagtt attaaactga tggaaccata ccctttaatg agcgggtaaa
    18541 tgtcaaagac agtaaaggaa tctacattta gtgcgaaaat attgaaatga tttaaaagta
    18601 aaaagagtac gacacttagt gtaaatgata taagaatatg ccatttatat ttagcactag
    18661 caacgatttg cgaacgtatc attggaataa acgcatcttc atgcatcaga cgaaaaatag
    18721 ctagtgaaat aataactgcg agtaaatagc taatgttcat tgaaatagga aaagagaaac
    18781 cccacggagc ttgttgagtg aatacagcta ctaacccaaa agttaaaaag acgataatga
    18841 tcggcaagat gttaaccaaa aatatgtaaa ggaaaataaa tccaatatca cgtttgaaaa
    18901 aacgcgattg ttcggtagcg tattcttctt ctatgtaatg tttatttgta tttgacatag
    18961 tatacctctt aaatagttgt attatataga tactttagca catattactt tgtattgtat
    19021 gttttataca ttaaaattta aaatgaaaaa catatcataa aattgtttta taaaatgaag
    19081 cgcttccatt gtgttttgtt ttgtaaggtg tatcataaat attgaattga aattttgggg
    19141 ggaggtattg taatgacgtt tcttacagtc atgcaattta tagttaacat tatcgttgta
    19201 ggattcatgc ttacggttat tgttatcggg cttatttggt taattaaaga taaaagacaa
    19261 tcacaacata gtgtattaag gaattatcct ttactagcac gtattagata tatttcagaa
    19321 aaaatgggac cggaattacg tcagtattta ttttctgggg ataatgaagg gaaacctttt
    19381 tcacgtaatg attataaaaa tatcgttttg gctggaaaat ataactctcg tatgaccagc
    19441 ttcggtacta ctaaagatta tcaagacggc ttttacatac agaacacaat gtttccgatg
    19501 caacgtaatg agatttcagt agataataca acattgttat caacattcat ttataaaatc
    19561 gcgaatgagc gtttatttag tcgtgaagaa tatcgtgtgc cgacaaagat tgatccgtat
    19621 tacttaagtg atgaccatgc aataaaatta ggtgaacatt taaaacatcc atttatttta
    19681 aaacgtatcg taggacaatc tggtatgagt tatggcgctt taggaaaaaa tgccattaca
    19741 gctttatcta aaggtctagc taaagcgggc acttggatga atacaggtga aggtggctta
    19801 tcagaatatc atttaaaagg taatggggat atcattttcc aaattggtcc cggtttattt
    19861 ggtgttcgtg ataaagaagg taattttagt gaaggtttat ttaaagaggt tgcacagtta
    19921 tctaacgtac gcgcatttga gctgaagttg gcacaaggtg ctaagactcg tggtggtcat
    19981 atggaagctg aaaaggtaaa cgaagaaatt gctaaaatcc gaaatgttga accttataaa
    20041 acaatcaatt cacctaaccg ttacgaattt attcataatg ctgaagattt gattcgtttc
    20101 gtcgatcagt tgcagcaatt aggtcaaaaa ccagtaggat tcaaaattgt agtaagcaaa
    20161 gtttcagaaa ttgaaacact tgtacgtacg atggtggaac tagataagta tccaagcttt
    20221 attacgattg atggtggtga aggtggtact ggtgcaacat tccaagaatt acaagatggt
    20281 gttggcttac cgctatttac agctctacct attgtgtctg gcatgttaga aaaatatggt
    20341 attcgagata aagtgaaatt ggcggcatct ggtaagttag tgacaccaga taaaattgcg
    20401 attgcactag gtttaggtgc agattttgta aatatcgcac gtgggatgat gattagtgtc
    20461 ggttgtataa tgagtcaaca atgtcacatg aatacgtgtc ctgtaggtgt tgcaacgaca
    20521 gatgcgaaga aagaaaaagc attgattgtt ggagaaaagc aatatcgtgt cacaaactat
    20581 gtaacaagtt tgcatgaagg cttattcaat attgcagcag ctgttggcgt atccagtcct
    20641 acagaaatta ctgctgatca tattgtatat cgaaaagtcg atggtgagtt acaaacgata
    20701 catgattata aattaaaact cattagttaa cttaattatt tcgggaaatt gaaagcagcg
    20761 gattttagcg ttactgcaaa taattttata ttagtagtgg atgctggtca cacaagaact
    20821 tcaaatatta aagccctcag aatatgaatt aaggtttgta accttagtct tatctgaggg
    20881 catttttaag ttataaacta tttgtcgtcc attttatctt tttcttttaa acctctgtgc
    20941 tttaattgct tttcaagttt ttcaaaacta atatctttat tttctttagt cgaaacacca
    21001 agacgtttat ttaatttttt catgtcaact tctgtgtaat ctatgtctaa gtgttcaatt
    21061 gcttttttat ctttatagtc tactttgtat tttacgcctt taaggtcttt gaaaatactt
    21121 tcagatttgg cgaataactt tttggcttcg tctttatcca tacctagatc gtcatattta
    21181 attgtgttga ttgtagactg ttttaaaact ttatcatctt tatatgtgat agaagttagt
    21241 acatgtttac cactaacatc accttcatat gttttggttt gttctttacc acaagctgat
    21301 aatgcaatga tacaaactaa tgctactaca attaatgaac ataatttttt caaagtcagt
    21361 cgccttcttt cgatatttgt attataaaga aattataaca tttactaaaa aatgatgtta
    21421 ttcaaaaatt taaattttgt catttttttt gaagatatga gtttttttaa gcggattcct
    21481 cacaaaattt taaaaatatt taagcctgaa aatgataaag cggtagggaa cgtagtgtgt
    21541 tccactgctg atttcttttt gagcattata ggcggtatgt tggaagaaaa gaacaaaagt
    21601 ttgtgaacga tagcatatag gtattaagaa tacgatgaac tcgttggtaa tattgacgta
    21661 ctcaagtgcg tacgctataa ttagcgtaac agaaaacaaa ggagggttta tatgattatc
    21721 actagcccta cagaagcgag aaaagatttt tatcaattac taaaaaatgt taataataat
    21781 cacgaaccaa tttatattag tggcaataat gccgaaaata atgctgtgat tataggttta
    21841 gaagattgga aaagtataca agagacaata tatcttgaat ctactggtac aatggacaaa
    21901 gtaagagaaa gagaaaaaga taatagtggt acaacaaata tagatgatat tgattgggat
    21961 aatctttaat gagcaattac acggttaaga ttaaaaattc agcgaaatca gatttaaaga
    22021 aaataaaaca ttcttattta aagaagtcat ttttagaaat tgttgagact ttaaaaaatg
    22081 atccgtataa aataacacaa tcttttgaaa aattagagcc taaatattta gagcgatatt
    22141 caagaagaat taaccatcag cacagggtcg tctataccgt agatgatcga aataaagaag
    22201 tattaatact atcggcatgg tcacattatg attaatgaat attcaatatc tgaataactt
    22261 taatgataag ttaattaaag aaactagtat ttaagtgtag ggaaaatagc gacgttaatg
    22321 cgttgttatc tctacacttt ttaattttat aatagcgcaa gactaaacag attgaaatta
    22381 gtaacaataa aagaataacg tattataata aggaatttta aattgtgact ttttcggaat
    22441 attaaatttt agaaatatga ggtttttaag cggattcctc acaaaatttt aaaaatattt
    22501 aagcctgaaa atgataaagc ggtagggaac gtttttctga aagttagtga tacaatagtt
    22561 ttaagttgaa atacaggagg atgaataaca tgaatcagtc agtcaaatta cttaaacatt
    22621 taacagatgt aaacggcatt gctggttatg aaatgcaagt taaagaagca atgcgtaact
    22681 atatagagcc tgtcagtgat caaattattg aagataactt gggtggcatt tttggaaaga
    22741 aaaatgctga gaatggtcaa tactcaatta tgatttctgg tcatatggat gaagttggtt
    22801 ttatggtaac aaagattgat aaacatggtt ttatttcatt tacgccagtt ggtggatggt
    22861 ggaatcaagt catgctatct caaaaagtaa cgattacaac agattcgggc aaagaaatta
    22921 gaggtatcat cggttctaaa ccgccacatg tcttaacgcc tgaagaacgt aaaaagccaa
    22981 tggaaatcaa aaatatgttt atagatattg gtgttagtag caaggaagaa gctgaagaag
    23041 ctggcgttga agtaggcaat atggttacgc catatagtga atttgaagtg cttgcaaatg
    23101 ataaatattt aactgcgaaa gcatttgata atcgctatgg ctgtgcatta gctattgagg
    23161 tattaaaacg tttaaaagat gaaaatattg gcattaactt atacagtggt gccacagtgc
    23221 aagaagaagt tggtttgcgt ggtgcgaaag tggcagcgaa tacgattaaa ccagacttgg
    23281 cgatagctgt cgatgtaggt attgcttatg ataccccagg tatgtcaggt caaacgagcg
    23341 atagtaaact aggcggtggt ccagttgtca ttatgatgga tgctacaagt attgctcacc
    23401 aaggtttgcg aaagcatatt aaagatgtag ctaaggaaca taacatcgaa gtacaatggg
    23461 atacgacacc aggtggaggt acagatgcgg gaagtattca tgtcgcaaat gaaggtattc
    23521 caacgatgac aatcggtgtt acgctgcgat acatgcattc taatgtttca gtgctcaatg
    23581 tagatgatta tgaaaattct atccgtcttg ttactgaaat tgtccgttca ttgaatgatg
    23641 aaagttataa aaatatcatg tggtaatcaa atccataaat aataaagaat ccttttaata
    23701 tggtaggttg ttaaacaatt gtctaatttt aattcttagt cattagacag tatccatgtt
    23761 aataggattt tttgttttta atttaaatgc tgaaaatcaa ttatgcctaa attttgatat
    23821 tacaagaaaa tgattttttc ttaaatgtaa ttgcactaaa aaccaaaaaa acgggaataa
    23881 tatacctgat atattacatg aggagcggtg caaatgttgt tagaaattaa agatttagtg
    23941 tataaagcga gcgatagaat catactagat catatcagtc taaaagtaga taaaggcgag
    24001 agtattgcca ttataggtcc atcaggtagt ggtaaaagta catttcaaaa gcaaatatgt
    24061 aatttgttta gtccaactag tggagaactt tattttaaag gtaaacccta taatgattat
    24121 gacccggaag aattgcgtca acgaatcagt tatttgatgc agcaaagtga cttgtttggt
    24181 gaaacgattg aagataacat gatattccca tcacttgcac gtaatgataa atttgataga
    24241 aaacgtgcaa agcaattaat taaagatgtc ggtttgggac attatcaatt aagttcggaa
    24301 gtggaaaata tgtcgggtgg tgagcggcaa agaattgcta tagcgcgcca actgatgtat
    24361 acaccggata ttcttttatt agatgaatcg accagtgcat tagacgttaa taataaagaa
    24421 aagatagaaa atatcatttt taaattagca gatcaaggcg tggcaattat gtggattacc
    24481 cacagcgatg accaaagtat gcgacacttt caaaagcgta taacaattgt tgatggtcaa
    24541 atttctaata cagaggagtt gaatcaacat gagtaatacg gcacttggat tgacggcatt
    24601 gcttttagtc attccgatta tcatttcata taaagaaggt ttacatatta ttaaagattt
    24661 aattgttgcg acattacgag cagttgtgca attaatcatt ttgggatttt tgctgcatta
    24721 tatttttaaa ataaacgata aatggctgct tattttatgt gtattggtca ttattattaa
    24781 tgcatcatgg aatacaatta gtcgagcatc accagtgatg catcatgtgt tttggatatc
    24841 atttctagct atcttcattg gaacggcatt accgcttgca ggtactattg cgacaggggc
    24901 cattcaattt accgcaaatg aagttatacc tatcggcggc atgcttgcaa ataatggctt
    24961 gattgcaatt aatttagctt accagaattt agatcgtgca ttcgtacaag atggtactaa
    25021 tattgaatct aaattatcac ttgcagctac acctaaattg gcttctaaag gtgcaatacg
    25081 tgaaagtatt cgtttagcta tagtgccaac tattgattcg gttaaaacat atgggcttgt
    25141 gtcgattcct ggtatgatga caggcttaat tattggtggc gtaccacctt tacaagcgat
    25201 taaatttcaa ttgttagtcg tgtttattca tacaactgcg accattatgt ctgctttgat
    25261 tgcgacatat ttaagctatg gtcaattttt caatgcaaga catcaattag tagcacgaaa
    25321 tactgatgtt aagagtgaat catgatagat tttactgcat cagatttagg cattagtttt
    25381 aattggaaat gaagtgacgc gcacatatag tatcgctatt cattagcgca gcgaaaatat
    25441 tcataaaggc acgcatactt tgtagtcagt tatctgttct gacatataaa gcgtgcgtgc
    25501 ttttttggag ttattgttga aactgaagta attatacata attattaaat gacatacttg
    25561 tgttaatttt tcaaatactg aaaaacaatt tcaataattt tccaattaag cacagaaaat
    25621 taaagcaaaa tattatataa tagaacggtt atatataaaa ttgtgttgca cacatttttt
    25681 aataaatcgt tattctaagg gaaatgaata tcggaaattt tgtttgaaag gagttttaaa
    25741 ttgtcaatca tgcgactatt tacattcatt ttaagtattt ttatcgtagg aatggttgaa
    25801 atgatggttg caggaattat gaacttgatg agtcaggact tacatgtatc agaagctgtc
    25861 gttggtcaat tagtgacaat gtacgcttta acatttgcga tatgtggacc tattctggtt
    25921 aaattaacga accgtttttc atcaaggcct gtattattat ggacattact tatatttatc
    25981 attggtaatg gcattattgc tgtagcgcca aatttttcaa tattagtagt tggtagaatt
    26041 atctcatctg cagcagcagc actaattatc gtaaaagtat tagctattac agcgatgtta
    26101 tcagcaccta aaaatcgtgg taaaatgatt ggacttgtct atacagggtt tagtggtgct
    26161 aatgtttttg gtgtaccaat tggaacggtt atcggcgatt tagtaggttg gcgctataca
    26221 tttctattct taattattgt gagtattatt gttggcttct tgatgatgat ctatttaccg
    26281 aaggatcagg aaatacaacg aggccctgtg aatcatgaga caccatctca tgaaaatcat
    26341 gttacttcga aaatattaag acctgctgaa gtagcaaaat atttaattat tacgttttta
    26401 gtattgattg caaactcagt gacattcgtc tttataaatc cacttatttt atctaatggt
    26461 catgatatgt cattcgtttc attagcactt ctagtaaatg gaatcgctgg cgttattgga
    26521 acatcattag gtggtatatt ctccgataaa attacaagta agcgttggtt aatgatttct
    26581 gtttctattt ttatcgtcat gatgttactt atgaatttaa tcttacctgg ttcaggtcta
    26641 ttgttagcag gactatttat ttggaatatc atgcaatgga gtactaatcc agcagtgcaa
    26701 agcggtgtga ttcaacatgt tgaaggcgac acaagccaag taatgagttg gaacatgtct
    26761 agtttaaacg ctggtattgg tgttggaggc attattggag gcttggtcat gacacatgtt
    26821 tctgttcaag ctatcacata tacgagtgcc atcattggcg cattaggatt aatcgttgtt
    26881 ttcacattga aaaataatca ttatgctaaa acatttaaat catcataatt ctcatatgaa
    26941 aagcacgcct gctatcaaat tcaggtgtgc ttttttagat gcgataacgt tattgatatg
    27001 tgcgataata gcgacgttca ttatgataca tcggccaagg cattttaccg cttttagcaa
    27061 aattagctaa atcattttgc atttgtcgac ttaaaaattt aaggtgagca gttgttggat
    27121 attgatgtgc tgctaatatt tgtaagtttc cgaaccaaaa taccatatct agaatatggt
    27181 aagcactgcg atagtgtgcg ctagaagtat catgccatgc aaattcagca agccatagtt
    27241 gtgcgtttga atcttgtata ttgagttgtt gtaaaaattg cttcatcggc tgtttgaaat
    27301 aacaatgtgt aatcgctaaa gattgttgct tcgccgtctg aacatcttcg tattttaaag
    27361 gaatatcatt taattccata atgtcgataa agcgttgcgg cgataatttt ttctgttcac
    27421 tagtaatata aatatcgcct tcatcttttg tataacatgc aaaaattggt ttcgttgttg
    27481 gataattatt ttgtatataa tctgttttaa taggcgcata tattaaatca agccctttag
    27541 aaggtcctcg agattgtttt agcttcgcca tcagcataag aatatcattt gtcgataatg
    27601 atgtaacatc atctgtatct aaataatcga gcatcatttt ttggaaatgt tgtgctttat
    27661 tgcgtgcact ctcaagggtg tctaatcgta gtgcgccact tagtagaacg actttatgga
    27721 agtatggctc aatgtcaggt attttaagta aagtcaaaat gctcatactg cctgcagact
    27781 gacccattaa agtaatgtta ttagcgtcgc caccgaagga ttcaataaat tgatgcaccc
    27841 attttatgac attgatttga tctgaaaggc cattattgga atgaaaatct ttattaaaat
    27901 atgaccagtc taaatatcct aatgcgccta aacgataatt gcatgtaata acgataatgt
    27961 cgttattttg tactaaatgt gccggttgat agagttcggc tgtaccatga ccattttcaa
    28021 aactaccacc ataaaaataa atgatgacag gtttcttcgt ctgatcatta tgttgtttcc
    28081 aaatatttag atataaacag tcttcatgtt cagtaaaagt tgtagattgt gaggagaaaa
    28141 aatcttctaa tttgttgtct ggttgcggtg ggatgggttg tatttcagtt gcatcaattg
    28201 gctctgacca ttgtgttttt aacgtagaat gtttaaagcg attgtcatga actggtggtt
    28261 ctgcataagg aatgcctaag aagatatcta aaccatcttg tgtaatacca tgaatttgac
    28321 cacctgtagt attaatcttc atatttatta acctccttta tcatccccaa tttaatcata
    28381 ccacggtcta tagatgatag gtgacaagta taacaagaca attttgtgac tgaatcagac
    28441 gagcttgata tctgttttaa ctcaaaccat tttcagcatt atatgatgta cttctacggg
    28501 agtgggacag aaatgataaa gagccacaaa tgatttatta tgtagtggtt cttatacatt
    28561 agccacagct aatgtgtact taaaaatatg aatacatgag taaaactcat gcataagaaa
    28621 tactaatttc tatagaaaaa gtatttcttt atcgtcgtcc caccccaact tgcattgcct
    28681 gtagaatttc ttttcgaaat tctctgtgtt ggggcccctg actagaatcg aaaaaagctt
    28741 gttacaagcg cattttcgtt cagtcaacta ctgccaatat aacttcgtag agcatagaac
    28801 attgatttat gtcccagcct gatcatcatc ttgttatgtt agttatttat aaccgatttt
    28861 caaaataaaa aaccaaccaa gatgttgaaa tcaatggttg gttaatgtgt gtgtgttgaa
    28921 atcatatatg gcactgtatt ttaaaatatt taatgagatg actttgattc attatttagt
    28981 gactcacgca tcttcgtatt gattcgtgtt gcgcgacgga aatagacgct ttcaaatgaa
    29041 ctcgttgtac cgatgtaata gaagataagt gccacaataa taataacgat aaagtcaaat
    29101 ggatagtgaa tcatatttaa gcctttaaat tctttgcttc ctataaatga cagtattgat
    29161 agcacgatta aatatacaat aatccataag ctaccaccaa tttgtttctt tgtattacgc
    29221 caattcatac gatattcata gaagaagtag attggtaatc caagtataat gattaaaata
    29281 acttcagccg ttgttggcca cattgcccaa tatatagcta atgaagctaa tacaaatgat
    29341 aatggtgcca ttacttttaa aatttttgct ctaaatggac gagtcattgt tggtcccatt
    29401 tttcttaatg caatcactgt cgttgggcca gttaaatagg ctactaaagt tgcagtagaa
    29461 ataactgctg ctagcgtacc ccaatctcta aataatgtaa ccataatcat actaatgatg
    29521 gcattaaaga tgattgctac acgtgggata tgatattttt cattcatctt cccaagaaat
    29581 ttagggatat gtccattttt ctccattgct cgtaaaactc gacctgtaac ggcgacaaat
    29641 gacacgccag taccgaatgg tgatacaaaa gcttcaatgt atagtaaaat tgcgagccaa
    29701 ttaattccta ataagatagc taaatcagca aatggtgaat tgaagttgat gccattccat
    29761 ccactatgtt gtaacattga ttgaggcata gaagtgataa acgtactttg taaaatgatg
    29821 tacaacactg cactgattga cagtgagata gcgatgcctc ttgcgatatt tttttcagga
    29881 tttttaattt ctgaacccat attaataatt gtctggaatg aattgaatga aaaaataatc
    29941 cctgatgctg ttgttgcagc aaaaatcggt gcacttccgt aaggcataaa tgtgcttgcc
    30001 gaatggccgt aatttgaagt gtcgaatcct gatagcatca acatgatgat ggttaacatg
    30061 ggtacgccta atttaaatac agaaattaaa ctcgtaaatg atgttaaaag ttttacggac
    30121 caatagttta ataatgaaaa aataacaatg atgagatata cagctagcaa tccgtatgtg
    30181 ctaatagagc cattttccat taaatatctc attggtttcg cccaatgcca cggccaagaa
    30241 ctcatatatt gaacagctga cacagcttcg ataggtatta ttgtcaccaa agacacccaa
    30301 ttcgcccaag cagcaataaa gcctaataat gagccatgtg tatactgggc atagttactc
    30361 atgccacctg attgaggaaa cattgtgccg atttcaatgt agttataggc aatggttcca
    30421 atgactagga atccaagaac ccatgatatg attgctgctg gtccagctat tgatgatgct
    30481 tcccacgcac caaatagcca accagatcct attaaagagc ctaaccctaa taagactaat
    30541 tgggatagat tgattttgtc gttgtgcaat tgcttgtctt tattcataaa tactccttcg
    30601 ttattgccaa aaatacaaaa aataatatgc gacaattata cgcttattat aataatagtc
    30661 aaatgcattt tttgtaagaa cgcaaaagca tttgtaaaat tagtttttta taaaattagg
    30721 atatataata caatgtgtat tgaaaaacaa aatggaagtg atgactatga gtgagaaaca
    30781 atcaaatgaa caagttgaag catttttaaa taaagagagt caatggcaag aagaatataa
    30841 atatttgaga gcgttaatct ttaatgaaac agaattagag gaagcgtata aatggatgca
    30901 tccttgttac acgttgaata ataaaaatgt agtacttatc catggcttca aaaattatgt
    30961 tgcactatta tttcataaag gtgccatttt ggaggataaa tatcatacac tcattcaaca
    31021 gactgaaaag gtgcaagcag ctcgtcagtt acgatttgaa aatttaacag agattcaagc
    31081 acgtaccgaa gaaattaaat attatctagc cgaagcaatt aaagctgaaa aagctggtaa
    31141 aaaagttgaa atgaagaaaa cagaggaata tgttattcct aaagagttag aagctaagtt
    31201 cgaagaaatg ccacagttag agtcttcatt ttataaatta acgccaggca gacaacatca
    31261 atatatatat catattggac aagctaaacg cagtgaaaca agacaaaagc gtgttgaaaa
    31321 gtatattaac caaatactag aaggtaaagg gatgcatgat aagtaattaa tgagtaaagc
    31381 ataccggtta tacaacaaca tacaagatga cacgaaacaa ccaatggctc atgctgttgg
    31441 ttgttttttt aggtgtgtct gtcatgggca acactttgac gttggaattc cgttacaggc
    31501 ttgggagtag aaaatgttag caaaaggcaa gggtgtctac aatgaatgat gaagatatta
    31561 aaatataagg atgactttgt gagtggcgga tgggcggttg tccgtctgta acaatggatg
    31621 cgtgtgcatt attacaaaaa ttcgactttt gtaataatat ttcacatttt cgacactttt
    31681 ttgctataaa acaaccaatt gagcgataat aaattcgctt ttaaaaaata tgagttatct
    31741 atttagttgc caaagataaa ataataatgt ttaataacat catatagagt atgttagttt
    31801 taaatgtcga atatacgaat gtgcaaacaa agtaatcggt agaaattcaa catacatagc
    31861 gccgtttact gttaagtatt cacattacag atgaaaaata taaaattcta cataatcaag
    31921 accatgatgt gtacttgttt aacttatgac tctatttgtt taacaattgc gataatggtc
    31981 tttttatttt atgcgtatca ttcgtcatat tttttatgag gaaggagaaa tgattatgtt
    32041 aagtattaag catttaacga aaatttattc tggtaataaa aaggcagtag atgacatctc
    32101 tttagatatt caatctgggg aatttatcgc atttattgga accagtggaa gtggcaaaac
    32161 gactgcttta agaatgataa accgtatgat tgaagcgaca gaaggacaaa ttgaaattga
    32221 tggtaaagat gttcggagta tgaatcctgt cgaattgcgt agaaatattg gctatgttat
    32281 tcaacaaatt ggcttaatgc ctcatatgac gattaaagag aatattgtgt tggtacccaa
    32341 attgttgaaa tggactaaag aggaaaagga taaacgtgca aaggaattaa ttaaacttgt
    32401 ggatttaccg gagtcatttt tagagcgtta tccagcagaa atatcaggtg ggcaacaaca
    32461 acgtatcggt gttgtaagag cacttgcggc cgaacaagat attattttaa tggatgaacc
    32521 ttttggtgca ttggatccta ttacgagaga tacgttacaa gatttagtta aaacgttaca
    32581 acgaaaatta ggcaagacgt ttatctttgt aacacatgat atggatgaag cgattaaatt
    32641 agcagacaaa atttgtatta tgtcagaagg taaggtggtg caatttgata cgccagacaa
    32701 tattttaaga catcccgcaa atgattttgt acgtgatttt ataggacaaa atagactgat
    32761 tcaagaccgt cccaatgaca agactgtaga aggtgtaatg attaaaccaa tcacgataca
    32821 agcagaagca acactgaatg acgccgttca tattatgaga caaaaacgtg ttgatgctat
    32881 ttttgtagta gatagtaata accatttact aggtttctta gacattgaag atataaatca
    32941 gggtatacgt ggacacaaaa gtttacgaga caccatgcaa caacatattt ataccgttca
    33001 aattgattct aaattacaag attctgtacg tacgatttta aaaagaaacg ttaggaatgt
    33061 acctgtcgta gatgatcaac agcgtttagt aggactgatt acgcgtgcca atgttgttga
    33121 tattgtatat gacacgattt ggggcgatag tgaggataca gtgcaaacag aacatgtggg
    33181 ggaagacact gcgtcctcaa aagtgcatga gcaacacact actaatgtca aagtacgtga
    33241 cataggagat gataaatcat gattgagttc ctacatgaac atggtggaca gttgatgtcg
    33301 aaaacactgg aacatttcta tatttctata gtggcattat tacttgccat cattgttgca
    33361 gtacctatag gcattttatt atcaaaaaca aagcgaactg ccaatattgt attaactgtg
    33421 gcaggtgtct tacaaactat tccaacacta gctgtacttg ctattatgat accgattttt
    33481 ggtgttggta aaacgcctgc aattgtagcg ctatttattt atgtattatt acctatttta
    33541 aataacacgg tactcggtgt tcaaaatatt gatagcaaca ttaaagaagc tggaaaaagt
    33601 atgggaatga cacaatttca attgatgaag gatgttgaat tgccgttagc attgccgctt
    33661 atcattggtg gcattcgttt gtcatctgtg tatgtaatta gttgggctac acttgcaagt
    33721 tatgtaggtg cgggtggatt aggtgatttc attttcaatg gtttaaattt atatgatcca
    33781 ctgatgattg taactgcaac ggtactcgtt actgcactag cattaggtgt tgatgcctta
    33841 ttagctttag ttgaaaaatg ggtagttccc aaaggcttaa aagtatctgg ataattagga
    33901 ggctaagata atgaagaaaa ttaaatatat acttgtcgtg tttgtcttat cgcttaccgt
    33961 attatctgga tgtagtttgc ccggactagg tagtaagagc acgaaaaatg atgtcaaaat
    34021 tacagcatta tcaacaagcg aatcgcaaat tatttcacat atgttacggt tgttaataga
    34081 gcatgataca cacggtaaga taaagccaac attagtaaat aatttagggt caagtacgat
    34141 tcaacataat gccttaatta atggggatgc taatatatca ggtgttagat ataatggcac
    34201 agatttaacg ggagctttga aggaagcacc aattaaaaat cctaagaaag caatgatagc
    34261 aacacaacaa ggatttaaaa agaaatttga tcaaacgttt tttgattcgt atggttttgc
    34321 gaatacgtat gcattcatgg taacgaagga aaccgctaaa aaatatcatt tagagacagt
    34381 ttcagattta gcaaagcata gtaaagattt acgtttaggt atggatagtt catggatgaa
    34441 tcgtaaaggc gatggctatg aaggatttaa aaaagagtat ggttttgact ttggtacagt
    34501 gagaccaatg caaataggtc tagtctacga cgcattaaac tcagagaagt tagacgttgc
    34561 attaggttat tctacagatg gtcgaattgc ggcgtatgat ttgaaagtac ttaaagatga
    34621 taaacaattt ttcccacctt atgctgcgag tgctgttgca acaaatgaat tattacggca
    34681 acacccagaa cttaaaacga cgattaataa gttgacagga aagatttcga cttcagagat
    34741 gcaacgcttg aattatgaag cggatggtaa aggtaaagaa cctgctgtcg tcgcagaaga
    34801 atttttgaaa aaacaccact attttgataa acagaaaggt ggtcataagt aatggaaggt
    34861 aatttattac agcaattatt caattattat gttacgaact ttggttatct atgggattta
    34921 tttttcaaac acttattaat gtctgtctat ggtgtgctgt ttgcagcttt aattggtatt
    34981 ccattgggaa tcttgcttgc aagatacaca aaactttctg gatttgtaat tacaattgca
    35041 aatataattc aaacagttcc agtcattgca atgttagcta ttttaatgtt agtcatgggc
    35101 ttaggttcag aaacagtagt tttaacagtg tttttatatg cgttacttcc aattataaaa
    35161 aacacttata ctggtatagc tagtgttgat gcgaatatta aggatgctgg caaaggtatg
    35221 ggaatgacac gcaatcaagt gctacgaatg attgaattac cgttatctgt ttcggttatt
    35281 atcggtggca ttcgtattgc cttggttgtt gcgataggtg ttgttgccgt tggatcattt
    35341 ataggagcac ctacgcttgg tgacattgtg attcgtggta caaatgcgac ggatggcaca
    35401 acgtttattt tagcaggtgc gattccgatt gctatcattg caatcgtcat tgatgtacta
    35461 ttaagatttt tagaaaaacg attagaccca acaacacgac atcgtaaaaa tcaatctaat
    35521 catcggccgc aaagtattaa tatgtaatag tagaagatgt ttataattta gcgatttcgt
    35581 ttcatgattt ataaaaaatg aggctactca aggagctcaa ataatctttg agtagccttt
    35641 ttataggttg tgtttgtatg cgtttacact aaaatagcaa ttattatcat gaaagttttt
    35701 ggataaaaag cgttaattat tgtaaaaata ctaaaaaatg agatgtttta tttataattt
    35761 tctgcaaatt tatgatattg tttcttaata tatcatatta aaaatttgtt tttcttaaac
    35821 ataggaggct tatctaattc atggacacat caaaacaatt tagaggtgac aaccgattgc
    35881 ttttgggtat cgttttaggg gttattacct tttggctatt cgcgcagtca cttgttaatc
    35941 ttgttgtccc attacaatca acatatagta gtgacgttgg aacgataaat atcgctgtta
    36001 gcttatctgc cttatttgct ggtttgttta tcgtaggtgc tggtgatgtt gctgataaat
    36061 ttggtcgcgt caaaattact tatgtaggat tgatattaaa tgttgtaggt tcattactca
    36121 tcatcattac acctttgcca gcatttttaa ttataggtag aataattcaa ggtttgtctg
    36181 cagcatgtat tatgccatca acacttgcta ttattaacga atattatatt ggtacaagaa
    36241 gacaacgtgc cttaagctat tggtctattg gttcttgggg tggtagtggt atttgtacgt
    36301 tgtttggtgg cttaatggct acatatatag gttggcgttc aatatttgtt gtttcaattc
    36361 tattaacatt attagcaatg tacttaatca aacatgcacc tgagactaaa gcagaaccaa
    36421 tcaaaggtat gaaagcagaa gctaaaaagt ttgacgttat tggtttagtc attttagtag
    36481 tgacgatgtt aagtttaaat gtaatcatca cacagacgtc tcattttggt ttagtttcac
    36541 cgttaattct aggtttaatt gttgtgttta tctgttcatt agttggtttt gtatattacg
    36601 aaaataaaat aaagcatcca cttgttgatt tttcaatttt taaaaataga ggatacagtg
    36661 gtgcaactat ttcaaacttc ttattaaatg gtgtagcagg tggtgcactt atcgttatta
    36721 acacgtatta tcaacaacaa ttaggattta attcttcgca aacgggttat atttcattaa
    36781 cgtatttaat aacagtgttg tcaatgattc gtgtaggtga aaagatttta tctcaacatg
    36841 gtccgaagcg cccactatta ctaggaagtg gctttacagt gattgggtta atcttattgt
    36901 cgttaacatt tttaccagaa gtgtggtata tcatatctag tatagttgga tatttattgt
    36961 ttggtactgg tttaggatta tatgctacac catcaactga tacagcagtt gctagtgcgc
    37021 cagatgataa gtcgggtgtt gcttcaggtg tgtataaaat ggcgtcatca ttaggaaatg
    37081 catttggagt agcagtatct ggtacggttt atactgtgtt agcagctaat ttaaatttga
    37141 acttaggtgg tttcacaggt atgatgttta atgccttgct agcaattgtt gcatttttag
    37201 tcattttact attagttcct aaaaatcaaa cgaatttgta aaactgaaat gaaagcaagt
    37261 tattatgtag ggattttaaa ggaaattttg tgaaagtaag tttatcatac acacttaatg
    37321 ttgcgtattg acgtttaatg ttaggtgtgt tcttttatag acgataaaag ctgtgtgcat
    37381 attaagcgaa tgattttcaa attgacgcta atatgcgaaa gtagtatttt taaaatgaac
    37441 aacaacgatg aagaggggtt tataggatga aaattgcaat tgctggatcg ggtgcattag
    37501 gtagtggctt tggtgccaaa ctatttcaag caggatatga tgtcacactt attgacggat
    37561 atacatctca tgttgaagcg gttaagcaac atggattaaa tataacgatt aatggagagg
    37621 cattcgagtt aaacattccg atgtatcatt ttaatgatca accggacgaa agcatttacg
    37681 atgttgtctt tctatttcca aagtctatgc aattaaaaga agtgatggaa gatatgaagc
    37741 cacatattga taatgaaacg atcgtcgtat gtacgatgaa tggtctgaag catgaagaag
    37801 tcattgcgca gtatgttgct caatcacaaa ttgtcagagg tgttacgact tggacggcag
    37861 gtcttgaaag ccctggacac agtcatttac ttggtagtgg accagttgaa ataggtgaac
    37921 tagtggatga aggtaaagaa aatgttataa aagttgctga tttacttaac gaagcggaat
    37981 tgaatggtgt cattagtaaa gatttatacc aatcgatttg gaaaaagatt tgtgttaatg
    38041 gtacggcaaa tgcattaagc acagtgttgg agtgtaatat ggcatcgctg aatgaaagta
    38101 gttatgcgaa gtgtttgatt tataaattaa cgcaagaaat agtgcatgta gcgacgattg
    38161 ataatgttca tttaaatgtt gatgaagtat ttgaatattt agttgattta aatgaaaaag
    38221 ttggtgcgca ttatccatcc atgtatcaag atttaattgt taataataga aaaactgaaa
    38281 ttgattatat taatggcgca gttgcaacat taggtaaaca acgtcatatt gaagcgccag
    38341 tcaatcgctt tattactgat ttaattcata ctaaagaaag tcagcgacac gcacaggatt
    38401 aattggtgtt ttaggtatga ttacaaggaa atgacaatca cgtgatatta cggtcattat
    38461 taagattgaa atgtaataaa taaagaacag cagtaaggta ctttcaaatt gaaatgatct
    38521 tggtgctgtt tttcttgatt gatcttcgtc ataattcaga tttgtcatag gtctacgaca
    38581 tactattagt atttactaga cagtttttac gacgacactt tgaaaaattt tgaggcaaat
    38641 catttggaag tctcacgtga attttgtaaa ctcatcaagc aagtaattat attaaaaaga
    38701 caaatagaga aaaggtgttt ataatgagta aaatttttgt aactggtgca acgggcctta
    38761 ttggcattaa attagttcaa agactaaaag aagaggggca tgaggttgct ggttttacta
    38821 catctgagaa tggtcaacaa aagctagctg ctgttaatgt aaaagcatat attggtgata
    38881 tattaaaagc tgatactatt gatcaagcgt tagcagattt taaaccagaa atcattatca
    38941 atcaaattac ggatttaaaa aatgttgata tggcagcaaa tacgaaagta cgtattgaag
    39001 gttctaaaaa cctaattgat gcggcgaaaa agcatgacgt taagaaagta attgcccaaa
    39061 gtattgcctt tatgtatgaa cctggcgaag gattagcaaa tgaggaaact tcacttgatt
    39121 ttaactcaac tggcgataga aaagtaacgg ttgatggtgt ggttggttta gaagaagaaa
    39181 cggctcgtat ggatgaatac gttgttttac gttttggctg gttatatggc ccaggtactt
    39241 ggtacggaaa agatggcatg atttataatc aatttatgga tggtcaagtg acactttcag
    39301 atggcgtaac atcatttgtg catcttgatg atgcagttga aacatctatt caagctattc
    39361 attttgaaaa tggtatctat aatgtagcag atgatgcacc tgttaaaggt tctgaatttg
    39421 cagaatggta taaagaacaa cttggtgttg aaccaaatat tgatattcaa cctgcgcaac
    39481 catttgaacg tggcgtaagc aatgagaagt ttaaagcgca aggtggtact ctgatttatc
    39541 aaacttggaa agatggcatg aatccaatta aataataatt tatccgttta atatacaaag
    39601 aataaagact tggtcgaatc gtggatgata tattatcaaa cgcacggctc gaacaagtct
    39661 tttttattat gtcttcgtta tctttgtatg aaggaataac agaattacaa ttaatgtact
    39721 gaataatgca attaatgttg tgattagtgc taatttaatt tctattggta gccaagtcag
    39781 tacaaaagac caattattgc taccgagaat gagatatggt aatgcatata atatgagcgc
    39841 taaagcgata catatacata atgataacca actcaataca gcaatccgtt ttgatctacg
    39901 caaaagaatt tgaccatggc gcatttgcca agcacgataa gctgagaata taaatgctaa
    39961 taaaataagt gttgtcatca aaacggtaac aatattaaat tgatctttat attgattgag
    40021 catagacgca accgtcgaat atcgcttgtg atttacaatt tgtgtattaa gatgctcaac
    40081 taatttgggt acatattccg aatttagatt tgcaagtaca acaattccat aattttgttt
    40141 tggatttagt aaaataaatg atgaaaagtt atctagcgtt cctgaatgaa acactaaatg
    40201 ttcatcatta ttggtaaacc agccggaagc atatgcattg gcattaggtt caccaattgt
    40261 tgaagataaa tttttatgtg attgttgaac taatgatttg tatttatcag gtggattaag
    40321 ttggaatttt atccaatgtt ccaaatcttc agttgatgtc atcatatatg ctgatggtgt
    40381 atcccaaagg ttaaattcag gtttagagac gacaggtgtc gaaccttgta attcatagcc
    40441 aatagcatca tgttttgatt tgtaattggt ttgtttgaat gatgtatgtg tcatatgcaa
    40501 aggcttgagc catgaatttg taatatattt tgtataggat tgcttcgtaa cgttttggat
    40561 aattaaacct aataaatcat agttcatatt tgagtattca aattcttctc cgggcttatg
    40621 atgtaattca tcacccataa ttgcacgggt tacatcattt aaacgattat ttttgcttgt
    40681 cacagaatct tcgcttgtaa tatcactagg tataccactt gtttgagcca aaagttgctt
    40741 aatcgtaata gtttcatttt gaccattata gttcatttta aaatgaggca catgtttgga
    40801 tacggcatca tttaagttta atcgaccttc ttgagctaat tttaaaattg caagacctgt
    40861 gaaagctttc gtattagaag caatttcata ctttgttgtg ggagaagctt tgactttttt
    40921 atcaacatct tgataaccat aacctttatt taaaaaaact ttgccatttt ttactattaa
    40981 aattgatgct ccaggaatgt gtcccttttg taaatcatgc tcgataattg tatctatttg
    41041 ttgttgcgaa tcattggtta accgtgtctt cgtattgcta tttaatgtta tatatatagc
    41101 aattgaaatg gcgactaaaa tgataataga aatggataga aaatacagtt ttttagtagt
    41161 cataaacgtt cctttttata tgttaaattt acttaagtgc ctaatgagat ttattttatt
    41221 ataccagttc tattgaataa attaattaaa gaggtatatt taccattatt tttgacacct
    41281 tatatgttaa taggtgtata attacataaa aaatattaat taactctcta tactttttgg
    41341 aaagataggc gtatagtgat atccatcaaa aactataaaa aggaatagga gattatgttc
    41401 aatcaattta aaagacttat tatagggcaa cctaaaaaaa acagagaact aaaagacgaa
    41461 aagatatcga agtttaaagg gttagcgatt ctttcgtcgg atgcattatc ttcagtggct
    41521 tacggtcccg aacaaatact gattacacta tcagtagtgg gtgcagttgc aacttggtat
    41581 actttaccga ttgcaggtgc tgttctgatc ttattagctg ctttaattat gtcttatagg
    41641 caaattattt atgcatatcc taaaggtggc ggcgcgtata tggtgtccaa gaccaattta
    41701 ggagaaaaat ggggattgct tgcaggaggc tcattattag tcgattacat actgactgtc
    41761 gctgttagta tatcatctgg tgctgatgca tttgttgctg cattcccaag tttatatggg
    41821 cataaagtac tgattgcatg tttacttgta ctttttattt taatattaaa tttacgtggt
    41881 ttaactgaat ctgccaccgt attatcctat ccagtatatc ttttcattat agggttagtg
    41941 atattaatat ttataggtac tttccgtgtg gcgacaggag atattcaacc acatatgcat
    42001 gcatcagtag gaactgcggt tcctggagta acattattct tgctattaaa agcattttca
    42061 tcaggggctt catcgttaac gggtgttgaa gctatttcaa atgcggtaac caattttaga
    42121 gagccaagtg ctaaaaatgc tgtgaaaacg ttaattgcta tgggttcaat tttagcgttt
    42181 ttattagttg gtatcgttgg tttagcatat gtttatggaa ttttgccaca aacggaaacg
    42241 acagttttat cacaattagc tatgcaaata tttggtgata atgctgcctt ctattttgta
    42301 caggcaacga ccgtgatgat tttggtatta gcagccaaca ctggatttac agcttttccg
    42361 atgttagcgg cttcaatgtc taaagataaa tatatgccta gaatgtttac tgttagaggt
    42421 gatcgcttag gctactctaa ctcgattatt attttaggtg tattagccat tattttaatt
    42481 attgtatttg atggtatgac tgaagattta attccattgt atgcggttgg tgtatttatt
    42541 ccatttacac tagcacaatt cggtatggtt attaaatgga ttcatgaacg tcctaagaat
    42601 tggttaagta agttgtctgt gaacttactt ggtggtatcg tgacatttat tgtttttatg
    42661 attttactca taactaaatt tagccaagta tggccgatac ttattttctt gccgttcgtg
    42721 gtgattttct tcttgaaaat taacaaacat tatcgagata tcgcagaaca attacgttct
    42781 gacattgatg ttcttaatgt ggacgtggta gatcgtaatt tagcaattgt gccgataaca
    42841 agtattacaa cagcagttga taagtctatt tattacgcac aaatgcttgc gaataatgat
    42901 gtcatagcag tacatgtatc atttggagat gaagatgaaa aagcattcca agagaaatgg
    42961 aaacgtcatt tcccagatgt aagattagtt attttacatt cggaatatcg cagtattata
    43021 cgaccaattt cacgttttat cgataagatt aatcgtaaag ccaacgatca aaactatatg
    43081 attacagtag tgataccaga atttattact aagaaacgtt ggcataattt attacacaat
    43141 caaactagtt tgcgtatgaa attgtattta atttatcaaa agaatgttaa cgtatgtaca
    43201 attccattta aacttaaaaa atagattcta taatcaaatc ggattgatga aaggttattt
    43261 acttttcatc aatctgattt tttgatgttg aatataaaaa ccccgtacat atgagatgat
    43321 aatgactttg tctcatatgt acgggggtat tgttatgtct tgcttaatgt ttggactgta
    43381 aagacaagac aatttcgttg taattgatat tttctcgaat ttgagctgca acgatattgt
    43441 tgattttatt tttgcgaatt aattcatcta aaagatgacg ttgtaagtac aatgcttcca
    43501 atttgtggtt atggaacata gcttgcggta tgatttgatt gctgttttct tgacgttctt
    43561 ttcgacgttg tgcacgccac tttaatgagc gactcatatc atagtaatga ttaatgacta
    43621 agctaacctc aagtacattt gtactatttt gttcttcttt taagcgtaaa ataatattgt
    43681 gattcacaat acgcataatt ttttgaacat ctttgaatga attttcaaat tcttgcttct
    43741 tagattcttt atttaaggct ttgtagtgct tcatcgcttt aagtctaagc cataacatct
    43801 tgaagaagtt tgcaaatttt tcaacaaatg atgcagtacg gtgtgtctct gttaattcga
    43861 caatattgcg gtaattatta atatcctgat atgttgcctt acctttatca attaaacgct
    43921 caagtgtaga tgtttcgatt acttttgcaa tatcttctaa acgtttgagt tcttttgtat
    43981 tttgattatc aggttctgaa tttaataaaa ataacaattc tccatagtat tggtttaata
    44041 ctgggcgata atttgtatcg tttttgtctt ttttactttc gtttttaaaa tgctggatca
    44101 ctttttgaac aatgaaaatt ttggcagatt gataagtcat acctttaaaa gtagtatctt
    44161 cggcagatgg tgtaattaaa ggtaaaacaa tttgcgctaa gattaaacta attaatacca
    44221 tgaaagatgc aataaacaat aaatcattac ggtattcgaa tgcttgtcct tttgtaatga
    44281 taaatggtaa tgtaagtgcc attgaaagtg aaattgtacc gtgaatacca cacatggtca
    44341 taataaatgc gtaacgcact cgagaaggtg gtgtttcatg tgaatcatgt tcctcgtcta
    44401 gataagattg tatatttttc gggaaataaa aatctttgta ccagaaataa acccaaacaa
    44461 atcgacatgc ataaattgca atagcgatta atatagtgat cactattaaa aatgaaaagt
    44521 tgtcaggctc tgtttggaaa atatctatga ctacagtagg aatcataaaa cctaatacaa
    44581 caaatacaaa accatttaag gcataactaa atgtgttcca aatttgatgg taattcattt
    44641 gtagttccgt ttgtgctcta attaaacggt cgcgttctaa accatggatt agacctgcga
    44701 ttacaactgc aatgatacct gaagcatgaa cttcttctgc taaaaagtat acgacaaaag
    44761 gagttaataa ttgaataaaa gttaaggtat tgttatcttt taaaccttta ttagcggtta
    44821 agtctatacg tattctaacg acaacgaatc caataattgc accaataagt acacctagta
    44881 ttgttgaaat gataaattgt tcaacagctt ggaataatga aaaggtacca gttactaatg
    44941 cagtaacagc aattttaaat gaaatgatac ctgctgcatc attgagtaaa gattcacctt
    45001 ctaaaatcgt catagaacct ttaggtaata attttccgcg tgtaatagca gatactgcta
    45061 ctgcatcagt aggacataaa attgctgcta ttgcaaaagc ggctggcatt ggtaaggcag
    45121 gccaaatcca atgtataaaa tagccaacac cgactacagt tgcaaacact agtgccattg
    45181 acattaatag tataggttta cgatattcta ataattttgt tcgagagacg tgggtacctt
    45241 ccacaaaaag tagtggcgcg ataacggcaa acataaatac ttcagaattg aattggaaat
    45301 caacttgtat tggaataatg aaaataacga cacctaatgc aatttgaata aaggcagtag
    45361 gaatttgtgg gaatcgatta ttgataaccg aactaataat cacagcaaaa ataaaaatta
    45421 aaaatgcttc
//
LOCUS       DAMQYT010000004        44545 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMQYT010000004 DAMQYT010000000
VERSION     DAMQYT010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 44545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44545
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.004"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            263..31528
                     /gene="ebh"
                     /locus_tag="PGA33_000178"
     CDS             263..31528
                     /gene="ebh"
                     /locus_tag="PGA33_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HDG6472019.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGFNTSQAHAAETNQ
                     PASVVKQKQQSNNEQTENRESQVQNSQNSQNGQSLSATHENEQPNISQANLVDQKVAQ
                     SSTTNDEQPASQNVNTKKDSATAATTQPDKEQSKHKQNESQSANKNGNDNRAAHVENH
                     EANVVTASDSSDNGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKIN
                     TLLGSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSNRIQTRSVESRAAEPRS
                     VSDYQNANSSYYVENANDGSGYPVGTYINASSKGAPYNLPTTPWNTLKASDSKEIALM
                     TAKQTGDGYQWVIKFNKGHAPHQNMIFWFALPADQVPVGRTDFVTVNSDGTNVQWSHG
                     AGAGANKPLQQMWEYGVNDPHRSHDFKIRNRSGQVIYDWPTVHIYSLEDLSRASDYFS
                     EAGATPATKAFGRQNFEYINGQKPAESPGVPKVYTFIGQGDASYTISFKTQGPTVNKL
                     YYAAGGRALEYNQLFMYSQLYVESTQDHQQRLNGLRQVVNRTYRIGTTKRVEVSQGNV
                     QTKKVLESTNLNIDDFVDDPLSYVKTPSNKVLGFYSNNANTNAFRPGGAQQLNEYQLS
                     QLFTDQKLQEAARTRNPIRLMIGFDYPDAYGNSETLVPVNLTVLPEIQHNIKFFKNDD
                     TQNIAEKPFSKQAGHPVFYVYAGNQGNASVNLGGSVTSIQPLRINLTSNENFTDKDWQ
                     ITGIPRTLHIENSTNRPNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTP
                     TITTTAEQLRGTALQKVPVNISGIPLDPSALVYLVAPTNQTTNGGSEADQIPSGYTIL
                     ATGTPDGVHNTITIRPQDYVVFIPPVGKQIRAVVYYNKVVASNMSNAVTILPDDIPPT
                     INNPVGINAKYYRGDEVNFTMGVSDRHSGIKNTTITTLPNGWTSNLTKADKNNGSLSI
                     TGRVSMNQAFNSDITFKVSATDNVNNTTNDSQSKHVSIHVGKISEDAHPIVLGNTEKV
                     VVVNPTAVSNDEKQSIITAFMNKNQNIRGYLASTDPVTVDNNGNVTLHYRDGSSTTLD
                     ATNVMTYEPVVKPEYQTVNAAKTATVTIAKGQSFSIGDIKQYFTLSNGQPIPSGTFTN
                     ITSDRTIPTAQEVSQMNAGTQLYHITATNAYHKDSEDFYISLKIIDVKQPEGDQRVYR
                     TSTYDLTTDEISKVKQAFINANRDVITLAEGDISVTNTPNGANVSTITVNINKGRLTK
                     SFASNLANMNFLRWVNFPQDYTVTWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLGT
                     QITTNDILTMLKATTTVPGLRNNITGNEKSQAEAGGRPNFRTTGYSQSNATTDGQRQF
                     TLNGQVIQVLDIINPSNGYGGQPVTNSNTRANHSNSTVVNVNEPAANGAGAFTIDHVV
                     KSNSTHNASDAVYKAQLYLTPYGPKQYVEHLNQNTGNTTDAINIYFVPSDLVNPTISV
                     GNYTNHQVFSGETFTNTITANDNFGVQSVTVPNTSQITGTVDNNHQHVSATAPNVTSA
                     TNKTINLLATDTSGNTATTSFNVTVKPLRDKYRVGTSSTAANPVRIANISNNATVSQA
                     DQTTIINSLTFTETVPNRSYARASANEITSKTVSNVSRTGNNANVTVTVTYQDGTTST
                     VTVPVKHVIPEIVAHSHYTVQGQDFPAGNGSSASDYFKLSNGSDIADATITWVSGQAP
                     NKDNTRIGEDITVTAHILIDGETTPITKTATYKVVRTVPKHVFETARGVLYPGVSDMY
                     DAKQYVKPVNNSWSTNAQHMNFQFVGTYGPNKDVVGISTRLIRVTYDNRQTEDLTILS
                     KVKPDPPRIDANSVTYKAGLTNQEIKVNNVLNNSSVKLFKADNTPLNVTNITHGSGFS
                     SVVTVSDALPNGGIKAKSSISMNNVTYTTQDEHGQVVTVTRNESVDSNDSATVTVTPQ
                     LQATTEGAVFIKGGDGFDFGHVERFIQNPPHGATVAWHDSPDTWKNTVGNTHKTAVVT
                     LPNGQGTRNVEVPVKVYPVANAKAPSRDVKGQNLTNGTDAMNYITFDPNTNTNGITAA
                     WANRQQPNNQQAGVQHLNVDVTYPGISAAKRVPVTVNVYQFEFPQTTYTTTVGGTLAS
                     GTQASGYAHMQNATGLPTDGFTYKWNRDTTGTNDANWSAMNKPNVAKVVNAKYDVIYN
                     GHTFATSLPAKFVVKDVQPAKPTVTETAAGAITIAPGANQTVNTHAGNVTTYADKLVI
                     KRNGNVVTTFTRRNNTSPWVKEASAATVAGIAGTNNGITVAAGTFNPADTIQVVATQG
                     SGETVSDEQRSDDFTVVAPQPNQATTKIWQNGHIDITPNNPSGHLINPTQAMDIAYTE
                     KVGNGAEHSKTINVVRGQNNQWTIANKPDYVTLDAQTGKVTFNANTIKPNSSITITPK
                     AGTGHSVSSNPSTLTAPAAHTVNTTEIVKDYGSNVTAAEINNAVQVANKRTATIKNGT
                     AMPTNLAGGSTTTIPVTVTYNDGSTEEVQESIFTKADKRELITAKNHLDDPVSTEGKK
                     PGTITQYNNAMHNAQQQINTAKTEAQQVINNERATPQQVSDALTKVRAAQTKIDQAKA
                     LLQNKEDNSQLVTSKNNLQSSVNQVPSTAGMTQQSIDNYNAKKREAETEITAAQRVID
                     NGDATAQQISDEKHRVDNALTALNQAKHDLTADTHALEQAVQQLNRTGTTTGKKPASI
                     TAYNNSIRALQSDLTSAKNSANAIIQKPIRTVQEVQSALTNVNRVNERLTQAINQLVP
                     LADNSALKTAKTKLDEEINKSVTTDGMTQSSIQAYENAKRAGQTESTNAQNVINNGDA
                     TDQQIAAEKTKVEEKYNSLKQAIAGLTPDLAPLQTAKTQLQNDIDQPTSTTGMTSASI
                     AAFNEKLSAARTKIQEIDRVLASHPDVATIRQNVTAANAAKSALDQARNGLTVDKAPL
                     ENAKNQLQHSIDTQTSTTGMTQDSINAYNAKLTAARNKIQQINQVLAGSPTVEQINTN
                     TSTANQAKSDLDHARQALTPDKAPLQTAKTQLEQSINQPTDTTGMTTASLNAYNQKLQ
                     AARQKLTEINQVLNGNPTVQNINDKVTEANQAKDQLNTARQGLTLDRQPALTTLHGAS
                     NLNQAQQNNFTQQINAAQNHAALETIKSNITALNTAMTKLKDSVADNNTIKSDQNYTD
                     ATPANKQAYDNAVNAAKGVIGETTNPTMDVNTVNQKAASVKSTKDALDGQQNLQRAKT
                     EATNAITHASDLNQAQKNALTQQVNSAQNVQAVNDIKQTTQSLNTAMTGLKRGVANHN
                     QVVQSDNYVNADTNKKNDYNNAYNHANDIINGNAQHPVITPSDVNNALSNVTSKEHAL
                     NGEAKLNAAKQEANTALGHLNNLNNAQRQNLQSQINGAHQIDAVNTIKQNATNLNSAM
                     GNLRQAVADKDQVKRTEDYADADTAKQNAYNSAVSSAETIINQTTNPTMSVDDVNRAT
                     SAVTSNKNALNGYEKLAQSKTDAARAIDALPHLNNAQKADVKSKINAASNIAGVNTVK
                     QQGTDLNTAMGNLQGAINDEQTTLNSQNYQDATPSKKTAYTNAVQAAKDILNKSNGQN
                     KTKDQVTEAMNQVNSAKNNLDGTRLLDQAKQTAKQQLNNMTHLTTAQKTNLTNQINSG
                     TTVAGVQTVQSNANTLDQAMNTLRQSIANKDATKASEDYVDANNDKQTAYNNAVAAAE
                     TIINANSNPEMNPSTITQKAEQVNSSKTALNGDENLAAAKQNAKTYLNTLTSITDAQK
                     NNLISQITSATRVSGVDTVKQNAQHLDQAMASLQNGINNESQVKSSEKYRDADTNKQQ
                     EYDNAITAAKAILNKSTGPNTAQNAVEAALQRVNNAKDALNGDAKLIAAQNAAKQHLG
                     TLTHITTAQRNDLTNQISQATNLAGVESVKQNANSLDGAMGNLQTAINDKSGTLASQN
                     FLDADEQKRNAYNQAVSAAETILNKQTGPNTAKTAVEQALNNVNNAKHALNGTQNLNN
                     AKQAAITAINGASDLNQKQKDALKAQANGAQRVSNAQDVQHNATELNTAMGTLKHAIA
                     DKTNTLASSKYVNADSTKQNAYTTKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAKQ
                     ELNGDERLREAKQNANTAIDALTQLNTPQKAKLKEQVGQANRLEDVQTVQTNGQALNN
                     AMKGLRDSIANETTVKTSQNYTDASPNNQSTYNSAVSNAKGIINQTNNPTMDTSAITQ
                     ATTQVNNAKNGLNGAENLRNAQNTAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVTA
                     TKNAATELNTQMGNLEQAIHDQNTVKQSVKFTDADKAKRDAYTNAVSRAEAILNKTQG
                     ANTSKQDVEAAIQNVSSAKNALNGDQNVTNAKNAAKNALNNLTSINNAQKRDLTTKID
                     QATTVAGVEAVSNTSTQLNTAMANLQNGINDKTNTLASENYHDADSDKKTAYTQAVTN
                     AENILNKNSGSNLDKTAVENALSQVANAKGALNGNHNLEQAKSNANTTINGLQHLTTA
                     QKDKLKQQVQQAQNVAGVDTVKSSANTLNGAMGTLRNSIQDNTATKNGQNYLDATERN
                     KTNYNNAVDSANGVINATSNPNMDANAINQIATQVTSTKNALDGTHNLTQAKQTATNA
                     IDGATNLNKAQKDALKAQVTSAQRVANVTSIQQTANELNTAMGQLQHGIDDENATKQT
                     QKYRDAEQSKKTAYDQAVAAAKAILNKQTGSNSDKAAVDRALQQVTSTKDALNGDAKL
                     AEAKAAAKQNLGTLNHITNAQRTDLEGQINQATTVDGVNTVKTNANTLDGAMNSLQGS
                     INDKDATLRNQNYLDADESKRNAYTQAVTAAEGILNKQTGGNTSKADVDNALNAVTRA
                     KAALNGADNLRNAKTSATNTIDGLPNLTQLQKDNLKHQVEQAQNVAGVNGVKDKGNTL
                     NTAMGALRTSIQNDNTTKTSQNYLDASDSNKNNYNTAVNNANGVINATNNPNMDANAI
                     NGMANQVNTTKAALNGAQNLAQAKTNATNTINNAHDLNQKQKDALKTQVNNAQRVSDA
                     NNVQHTATELNSAMTALKAAIADKERTKASGNYVNADQEKRQAYDSKVTNAENIISGT
                     PNATLTVNDVNSAASQVNAAKTALNGDNNLRVAKEHANNTIDGLAQLNNAQKAKLKEQ
                     VQSATTLDGVQTVKNSSQTLNTAMKGLRDSIANEATIKAGQNYTDASPNNRNEYDSAV
                     TAAKAIINQTSNPTMEPNTITQVTSQVTTKEQALNGARNLAQAKTTAKNNLNNLTSIN
                     NAQKDALTRSIDGATTVAGVNQETAKATELNNAMHSLQNGINDETQTKQTQKYLDAEP
                     SKKSAYDQAVNAAKAILTKASGQNVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAAK
                     QTLGTLTHINNAQRTALDNEITQATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTTL
                     QSQNYQDADDAKRTAYSQAVNAAATILNKTAGGNTPKADVERAMQAVTQANTALNGIQ
                     NLDRAKQAANTAITNASDLNTKQKEALKAQVTSAGRVSAANGVEHTATELNTAMTALK
                     RAIADKAETKASGNYVNADANKRQAYDEKVTAAENIVSGTPTPTLTPADVTNAATQVT
                     NAKTQLNGNHNLEVAKQNANTAIDGLTSLNGPQKAKLKEQVGQATTLPNVQTVRDNAQ
                     TLNTAMKGLRDSIANEATIKAGQNYTDASQNKQTDYNSAVTAAKAIIGQTTSPSMNAQ
                     EINQAKDQVTAKQQALNGQENLRTAQTNAKQHLNGLSDLTDAQKDAVKRQIEGATHVN
                     EVTQAQNNADALNTAMTNLKNGIQDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQILN
                     KAQGPNTSKDGVETALENVQRAKNELNGNQNVANAKTTAKNALNNLTSINNAQKEALK
                     SQIEGATTVAGVNQVSTTASELNTAMSNLQNGINDEAATKAAQKYTDADREKQTAYND
                     AVTAAKTLLDKTAGSNDNKAAVEQALQRVNTAKTALNGDERLNEAKNTAKQQVATMSH
                     LTDAQKANLTSQIESGTTVAGVQGIQANAGTLDQAMNQLRQSIASKDATKSSEDYQDA
                     NADLQNAYNDAVTNAEGIISATNNPEMNPDTINQKASQVNSAKSALNGDEKLAAAKQT
                     AKSDIGRLTDLNNAQRTAANAEVDQAPNLAAVTAAKNKATSLNTAMGNLKHALAEKDN
                     TKRSVNYTDADQPKQQAYDTAVTQAEAITNANGSNANETQVQAALNQLNQAKNDLNGD
                     NKVAQAKESAKRALASYSNLNNAQSTAAISQIDNATTVAGVTAAQNTANELNTAMGQL
                     QNGINDQNTVKQQVNFTDADQGKKDAYTNAVTNAQGILDKAHGQNMTKAQVEAALNQV
                     TTAKNALNGDANVRQAKSDAKANLGTLTHLNNAQKQDLTSQIEGATTVNGVNGVKTKA
                     QDLDGAMQRLQSAIANKDQTKASENYIDADPTKKTAFDNAITQAESYLNKDHGANKDK
                     QAVEQAIQSVTSTENALNGDANLQRAKTEAIQAIDNLTHLNTPQKTALKQQVNAAQRV
                     SGVTDLKNSATSLNNAMDQLKQAIADHDTIVASGNYTNASPDKQGAYTDAYNAAKNIV
                     NGSPNVITNAADVTAATQRVNNAETGLNGDTNLATAKQQAKDALRQMTHLSDAQKQSI
                     TGQIDSATQVTGVQSVKDNATNLDNAMNQLRNSIANKDDVKASQPYVDADRDKQNAYN
                     TAVTNAENIINATSQPTLDPSAVTQAANQVSTNKTALNGAQNLANKKQETTANINQLS
                     HLNNAQKQDLNTQVTNAPNISTVNQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFTD
                     ADPEKQTAYNNAVTAAENIINQANGTNANQSQVEAALSTVTTTKQALNGDRKVTDAKN
                     NANQTLSTLDNLNNAQKGAVTGNINQAHTVAEVTQAIQTAQELNTAMGNLKNSLNDKD
                     TTLGSQNFADADPEKKNAYNEAVHNAENILNKSTGTNVPKDQVEAAMNQVNATKAALN
                     GTQNLEKAKQHANTAIDGLSHLTNAQKEALKQLVQQSTTVAEAQGNEQKANNVDAAMD
                     KLRQSIADNATTKQNQNYTDASQNKKDAYNNAVTTAQGIIDQTTSPTLDPTVINQAAG
                     QVSTTKNALNGNENLEAAKQQASQSLGSLDNLNNAQKQTVTDQINGAHTVDEANQIKQ
                     NAQNLNTAMGNLKQAIADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGNA
                     TQAEVEQAIKQVNAAKQALNGNANVQHAKDEATALINSSNDLNQAQKDALKQQVQNAT
                     TVAGVNNVKQTAQELNNAMTQLKQGIADKEQTKADGNFVNADPDKQNAYNQAVAKAEA
                     LISATPDVVVTPSEITAALNKVTQAKNDLNGNTNLATAKQNVQHAIDQLPNLNQAQRD
                     EYSKQITQATLVPNVNAIQQAATTLNDAMTQLKQGIANKAQIKGSENYHDADTDKQTA
                     YDNAVTKAEELLKQTTNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQDAKTTLGT
                     LDHLNDAQKQALTTQVEQAPDIATVNNVKQNAQNLNNAMTNLNNALQDKTETLNSINF
                     TDADQAKKDAYTNAVSHAEGILSKANGSNASQTEVEQAMQRVNEAKQALNGNDNVQRA
                     KDAAKQVITNANDLNQAQKDALKQQVDAAQTVANVNTIKQTAQDLNQAMTQLKQGIAD
                     KDQTKANGNFVNADTDKQNAYNNAVAHAEQIISGTPNANVDPQQVAQALQQVNQAKGD
                     LNGNHNLQVAKDNANTAIDQLPNLNQPQKTALKDQVSHAELVTGVNAIKQNADALNNA
                     MGTLKQQIQANSQVPQSVDFTQADQDKQQAYNNAANQAQQIANGIPTPVLTPDTVTQA
                     VTTMNQAKDALNGDEKLAQAKQEALANLDTLRDLNQPQRDALRNQINQAQALATVEQT
                     KQNAQNVNTAMSNLKQGIANKDTVKASENYHDADADKQTAYTNAVSQAEGIINQTTNP
                     TLNPDEITRALTQVTDAKNGLNGEAKLATEKQNAKDAVSGMTHLNDAQKQALKGQIDQ
                     SPEIATVNQVKQTATSLDQAMDQLSQAINDKAQTLADGNYLNADPDKQNAYKQAVAKA
                     EALLNKQSGTNEVQAQVESITNEVNAAKQALNGNDNLANAKQQAKQQLANLTHLNDAQ
                     KQSFESQITQAPLVTDVTTINQKAQTLDHAMELLRNSVADNQTTLASEDYHDATAQRQ
                     NDYNQAVTAANNIINQTTSPTMNPDDVNGATTQVNNTKVALDGDENLAAAKQQANNRL
                     DQLDHLNNAQKQQLQSQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQAVEQRG
                     NFINADTDKQTAYNTAVNEAAAMINKQTGQNANQTEVEQAITKVQTTLQALNGDHNLQ
                     VAKTNATQAIDALTSLNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRQSI
                     QDNAATKANSKYINEDQPEQQNYDQAVQAANNIINEQTATLDNNAINQAATTVNTTKA
                     ALHGDVKLQNDKDHAKQTVSQLAHLNNAQKHMEDTLIDSETTRTAVKQDLTEAQALDQ
                     LMDALQQSIADKDATRASSAYVNAEPNKKQSYDEAVQNAESIIAGLNNPTINKGNVSS
                     ATQAVISSKNALDGVERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRGEVAQ
                     KLTEAQALNQAMEALRNSIQDQQQTEAGSKFINEDKPQKDAYQAAVQNAKDLINQTNN
                     PTLDKAQVEQLTQAVNQAKDNLHGDQKLADDKQHAVTDLNQLNGLNNPQRQALESQIN
                     NAATRGEVAQKLAEAKALDQAMQALRNSIQDQQQTESGSKFINEDKPQKDAYQAAVQN
                     AKDLINQTGNPTLDKSQVEQLTQAVTTAKDNLHGDQKLARDQQQAVTTVNALPNLNHA
                     QQQALTDAINAAPTRTEVAQHVQTATELDHAMETLKNKVDQVNTDKAQPNYTEASTDK
                     KEAVDQALQAAESITDPTNGSNANKDAVDQVLTKLQEKENELNGNERVAEAKTQAKQT
                     IDQLTHLNADQIATAKQNIDQATKLQPIAELVDQATQLNQSMDQLQQAVNEHANVEQT
                     VDYTQADSDKQNAYKQAIADAENVLKQNANKQQVDQALQNILNAKQALNGDERVALAK
                     TNGKHDIDQLNALNNAQQDGFKGRIDQSNDLNQIQQIVDEAKALNRAMDQLSQEITDN
                     EGRTKGSTNYVNADTQVKQVYDETVDKAKQALDKSTGQNLTAKQVIKLNDAVTAAKKA
                     LNGEERLNNRKAEALQRLDQLTHLNNAQRQLAIQQINNAETLNKASRAINRATKLDNA
                     MGAVQQYIDEQHLGVISSTNYINADDNLKANYDNAIANAAHELDKVQGNAIAKAEAEQ
                     LKQNIIDAQNALNGDQNLANAKDKANAFVNSLNGLNQQQQDLAHKAINNADTVSDVTD
                     IVNNQIDLNDAMETLKHLVDNEIPNAEQTVNYQNADDNAKTNFDDAKRLANTLLNSDN
                     TNVNDINGAIQAVNDAIHNLNGDQRLQDAKDKAIQSINQALANKLKEIEASNATDQDK
                     LIAKNKAEELANSIINNINKATSNQAVSQVQTAGNHAIEQVHANEIPKAKIDANKDVD
                     KQVQALIDEIDRNPNLTDKEKQALKDRINQILQQGHNGINNAMTKEEIEQAKAQLAQA
                     LQDIKDLVKAKEDAKQDVDKQVQALIDEIDQNPNLTDKEKQALKYRINQILQQGHNDI
                     NNALTKEEIEQAKAQLAQALQDIKDLVKAKEDAKNAIKALANAKRDQINSNPDLTPEQ
                     KAKALKEIDEAEKRALQNVENAQTIDQLNRGLNLGLDDIRNTHVWEVDEQPAVNEIFE
                     ATPEQILVNGELIVHRDDIITEQDILAHINLIDQLSAEVIDTPSTATISDSLTAKVEV
                     TLLDGSKVIVNVPVKVVEKELSVVKQQAIESIENAAQQKINEINNSVTLTLEQKEAAI
                     AEVNKLKQQAIDHVNNAPDVHSVEEIQQQEQAHIEQFNPEQFTIEQAKSNAIKSIEDA
                     IQHMIDEIKARTDLTDKEKQEAIAKLNQLKEQAIQAIQRAQSIDEISEQLEQFKAQMK
                     AANPTAKELAKRKQEAISRIKDFSNEKINSIRNSEIGTADEKQAAMNQINEIVLETIR
                     DINNAHTLQQVEAALNNGIARISAVQIVTSDRAKQSSSTGNESNSHLTIGYGTANHPF
                     NSSTIGHKKKLDEDDDIDPLHMRHFSNNFGNVIKNAIGVVGISGLLASFWFFIAKRRR
                     KEDEEEELEIRDNNKDSIKETLDDTKHLPLLFAKRRRKEDEEDVTVEEKDSLNNGESL
                     DKVKHTPFFLPKRRRKEDEEDVEVTNENTDEKVLKDNEHSPLLFAKRRKDKEEDVETT
                     TSIESKDEDVPLLLAKKKNQKDNQSKDKKSASKNTSKKVAAKKKKKKAKKNKK"
     gene            complement(31587..31988)
                     /locus_tag="PGA33_000179"
     CDS             complement(31587..31988)
                     /locus_tag="PGA33_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDG6472020.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVQNALLYTDSKLIADSIEAGYVKNANFKPYFDQIEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
     gene            complement(32529..33233)
                     /locus_tag="PGA33_000180"
     CDS             complement(32529..33233)
                     /locus_tag="PGA33_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HDG6472021.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            33478..33672
                     /locus_tag="PGA33_000181"
     CDS             33478..33672
                     /locus_tag="PGA33_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HDG6472022.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            33684..33935
                     /locus_tag="PGA33_000182"
     CDS             33684..33935
                     /locus_tag="PGA33_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HDG6472023.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            33973..35097
                     /locus_tag="PGA33_000183"
     CDS             33973..35097
                     /locus_tag="PGA33_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HDG6472024.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIQEVMDGVLEEVLTTYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPVVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            35113..35550
                     /locus_tag="PGA33_000184"
     CDS             35113..35550
                     /locus_tag="PGA33_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDG6472025.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            35974..36930
                     /locus_tag="PGA33_000185"
     CDS             35974..36930
                     /locus_tag="PGA33_000185"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDG6472026.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGGTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            37130..37609
                     /locus_tag="PGA33_000186"
     CDS             37130..37609
                     /locus_tag="PGA33_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDG6472027.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            37624..38463
                     /gene="fakB2"
                     /locus_tag="PGA33_000187"
     CDS             37624..38463
                     /gene="fakB2"
                     /locus_tag="PGA33_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HDG6472028.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            38549..39082
                     /gene="msrA"
                     /locus_tag="PGA33_000188"
     CDS             38549..39082
                     /gene="msrA"
                     /locus_tag="PGA33_000188"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDG6472029.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            39075..39503
                     /gene="msrB"
                     /locus_tag="PGA33_000189"
     CDS             39075..39503
                     /gene="msrB"
                     /locus_tag="PGA33_000189"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDG6472030.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            39515..40015
                     /locus_tag="PGA33_000190"
     CDS             39515..40015
                     /locus_tag="PGA33_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDG6472031.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            40015..40236
                     /locus_tag="PGA33_000191"
     CDS             40015..40236
                     /locus_tag="PGA33_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDG6472032.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            40428..41918
                     /locus_tag="PGA33_000192"
     CDS             40428..41918
                     /locus_tag="PGA33_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDG6472033.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDKALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
     gene            42318..42827
                     /locus_tag="PGA33_000193"
     CDS             42318..42827
                     /locus_tag="PGA33_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6472034.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            42839..43909
                     /locus_tag="PGA33_000194"
     CDS             42839..43909
                     /locus_tag="PGA33_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDG6472035.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTLIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            43926..44540
                     /locus_tag="PGA33_000195"
     CDS             43926..44540
                     /locus_tag="PGA33_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDG6472036.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFVYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGLFCIILSTLLLRNKLIN"
BASE COUNT        17622 a         7540 c         7986 g        11397 t
ORIGIN      
        1 attattataa attgtcacaa attcaattta ccttacaata tattttgtgt tattatattc
       61 tggagcataa ataaattgtt caacacatag ttgtaatgtg tttcaatact ttttggatag
      121 attgcgaaat tgtattgaat cgtcatcgtt ttaaattttt aaatgagaat ggaatgagca
      181 ttacaataca caagcaatca aaagtaaata cattcacaac acaacagaga cataacaaca
      241 agataaggag tgaacaatag ctgtgaatta tcgtgataaa attcaaaagt ttagtattcg
      301 taaatataca gttggtacat tttcaactgt cattgcgaca ttggtatttt taggattcaa
      361 tacatcacaa gcacatgctg ctgaaacaaa tcaaccagca agcgtggtta aacagaaaca
      421 acaaagtaat aatgaacaga ctgagaatcg agaatctcaa gtacaaaatt ctcaaaattc
      481 acaaaatggt caatcattat ctgctactca tgaaaatgag caaccaaata ttagtcaagc
      541 taatttagta gatcaaaaag tagcgcaatc atctactact aatgatgaac aaccagcatc
      601 tcaaaatgta aatacaaaga aagattcggc aacggctgcg acaacacaac cagataaaga
      661 acaaagtaag cataaacaaa acgaaagtca atctgctaat aaaaatggaa acgacaatag
      721 agcggctcat gtagaaaatc atgaagcaaa tgtagtaaca gcttcagatt catctgataa
      781 tggtaacgta caacatgacc gaaatgaatt acaagcgttt tttgatgcaa attatcatga
      841 ttatcgcttt attgaccgtg aaaatgcaga ttctggcaca tttaactatg taaaaggcat
      901 ttttgataag attaatacgt tattaggcag taatgatcca ataaacaata aagacttgca
      961 acttgcatac aaagaattgg aacaagctgt tgctttaatt cgtacaatgc ctcaacgtca
     1021 acagactagc cgacgttcaa atagaattca aacgcgttcg gttgagtcaa gagctgcaga
     1081 gcctagatca gtatcagact atcaaaatgc aaattcatca tattatgttg aaaatgctaa
     1141 tgatggttcg ggctatcctg ttggtacata tatcaatgct tctagtaaag gggcgccata
     1201 taatttacca actacaccat ggaatacatt gaaggcctct gactcaaagg aaattgctct
     1261 tatgacagcg aaacaaactg gagacgggta ccaatgggtt attaagttta ataaaggaca
     1321 tgctccacat caaaatatga tcttttggtt tgcattacca gcagaccaag tgccagtagg
     1381 aagaactgac tttgtaacag ttaattcaga tggaacaaat gtacaatgga gtcatggagc
     1441 aggagcaggt gcaaataaac cacttcaaca aatgtgggaa tatggagtaa atgatcctca
     1501 tcgttcacat gactttaaaa taagaaatag aagtggccaa gtaatatatg actggccaac
     1561 tgtccatatt tattctttag aagatttatc tagagcgagt gattatttta gtgaagctgg
     1621 agcgacacct gctactaaag cttttggtag acaaaatttt gaatatatta atggtcaaaa
     1681 acctgctgaa tcaccgggtg ttcctaaagt ttatactttc atcggtcaag gtgatgcaag
     1741 ttatacaatt tcatttaaaa cacaaggtcc aactgttaat aaattgtact atgcagcagg
     1801 tgggcgtgct ttagagtaca atcaattatt tatgtacagt caactatacg tcgaatcaac
     1861 gcaagaccat caacaacgtc ttaatggttt aagacaagtg gttaatcgta catatcgcat
     1921 aggtacaact aaacgtgtag aagtgagtca aggaaatgta caaacgaaaa aggtattaga
     1981 aagtacaaac ctaaatatag atgattttgt tgatgatcct ttaagttatg ttaagacgcc
     2041 gagtaataaa gtgttaggat tttattcgaa taatgcaaat actaatgctt ttagaccggg
     2101 tggagcccaa caattaaatg aatatcaatt aagtcaatta tttactgatc aaaaattaca
     2161 agaagcagca agaactagaa acccaataag attaatgatt ggtttcgact atcctgatgc
     2221 ttatggtaat agtgaaactt tagttcctgt taacttaacg gtattacctg aaatccaaca
     2281 taatattaaa ttctttaaaa atgacgatac tcaaaatatt gctgaaaaac cattttcaaa
     2341 acaagctggg catccagttt tctatgtata tgcaggtaac caagggaatg cttccgtgaa
     2401 tttaggtggt agcgtaacat ctattcaacc attacgtatt aatttaacaa gtaatgagaa
     2461 ttttacagat aaagattggc aaattacagg tattccgcgt acattacaca ttgaaaactc
     2521 gacaaataga cctaataatg ccagagaacg caatattgaa cttgttggta acttattacc
     2581 aggggattac tttggaacga tacgttttgg acgtaaagaa caattattcg aaattcgtgt
     2641 taaaccacat acaccaacaa ttacaacgac agctgagcaa ttaagaggta cagcattaca
     2701 aaaagtgcct gttaatattt cgggaatacc gttggatcca tcggcattgg tttatttagt
     2761 tgcaccaaca aatcaaacta cgaatggtgg tagtgaggca gatcaaatac catctggtta
     2821 tacgatactt gcgactggta cacctgatgg ggtgcataat acaattacta tacgaccgca
     2881 agattatgtt gtattcatac cacctgtagg taaacaaatt agagcagtag tttattataa
     2941 taaagtagtt gcatctaata tgagtaatgc tgttactatt ttgccagatg acattccacc
     3001 aacaatcaat aatcctgttg gaataaatgc caaatactat cgaggcgacg aagtcaactt
     3061 tacaatgggt gtctctgata gacattctgg tataaaaaat acaactatta cgacattgcc
     3121 gaatggttgg acatcaaatt taacaaaagc ggataagaat aatggctcat tatctattac
     3181 aggtagagtg agtatgaatc aggcatttaa cagtgatatt acatttaaag tgtcagcgac
     3241 agacaatgtc aataatacga caaatgatag tcaatctaaa catgtttcaa ttcatgtagg
     3301 taaaattagt gaagatgctc atccgattgt attaggaaat actgagaaag ttgtagtagt
     3361 caatccgact gctgtatcta atgatgaaaa gcaaagcata attactgcct ttatgaataa
     3421 aaaccaaaat ataagaggat atttagcatc aactgatcca gtaactgtcg ataataatgg
     3481 taatgtcaca ttacattacc gtgatggctc atcgacaacg cttgatgcta caaatgtgat
     3541 gacatacgaa ccagttgtga aacctgaata ccaaactgtc aatgctgcta aaacagcaac
     3601 ggtaacgatt gctaaaggac aatcatttag tattggtgat attaaacaat attttacttt
     3661 aagtaatgga caacctattc caagtggcac atttacaaat attacatctg atagaactat
     3721 tccaactgca caagaagtta gtcaaatgaa cgcaggcacg cagttatacc atataactgc
     3781 tacaaatgcg tatcataaag atagtgaaga cttctatatt agtttgaaaa tcatcgatgt
     3841 gaaacaacca gaaggcgatc aacgtgtata tcgtacatca acatatgatt taactactga
     3901 tgaaatctca aaagtaaaac aagcatttat taatgcaaat agagatgtaa ttacgcttgc
     3961 cgaaggtgat atttcagtta caaatacacc taatggtgct aatgtaagta ctattacagt
     4021 aaatattaat aaaggtcgat taacgaaatc attcgcgtca aacctagcta atatgaattt
     4081 cttgcgttgg gttaatttcc cacaagatta tacagtgaca tggacgaatg caaaaattgc
     4141 aaacagacca acagatggtg gtttatcatg gtctgatgac cataaatctt taatttatcg
     4201 ttatgatgct acattaggta ctcaaattac gacgaatgat attttaacaa tgttaaaagc
     4261 aacaactaca gtgcctggat tgcgaaataa cattactggt aatgaaaaat cacaagcaga
     4321 agctggcgga agacctaact ttagaacgac tggttattca caatcaaatg cgacaactga
     4381 tggtcaacgt caatttacgt tgaatggtca agtgattcaa gtgttagaca tcatcaaccc
     4441 ttcaaacggt tatggtgggc aacctgttac aaattcaaat actcgtgcaa accatagtaa
     4501 ctcaactgtt gttaacgtaa acgaaccggc agctaatggt gctggcgcat ttacaattga
     4561 ccacgttgta aaaagtaatt ctacacataa tgcaagtgat gcagtttata aagcacagtt
     4621 atacttaacg ccatatggtc caaaacaata tgttgaacat ttaaatcaaa atacaggaaa
     4681 tactactgac gctattaaca tttattttgt accaagtgac ttagtgaatc caacaatttc
     4741 agtaggtaat tacactaatc atcaagtgtt ctcaggtgaa acatttacaa atactattac
     4801 agcgaatgat aactttggtg tgcaatctgt aactgtacca aatacatcac aaattacagg
     4861 tactgttgat aataaccatc aacatgtttc tgcaacggca ccaaatgtga catcagcaac
     4921 taataagaca atcaatttat tagcaactga tacaagcggc aatacagcta caacttcgtt
     4981 caatgtaaca gtgaaacctt tgcgtgataa atatcgagtt ggtacttcat caacggctgc
     5041 taatcctgtg agaattgcca atatttcgaa taatgcgaca gtatcacaag ctgatcaaac
     5101 gacaattatt aattcgttaa cgtttactga aacagtacca aatagaagtt atgcaagagc
     5161 aagtgcgaat gaaatcacta gtaaaacagt tagtaatgtc agtcgtactg gaaataatgc
     5221 caatgtcaca gtaactgtta cttatcaaga tggaacaaca tcaacagtga ctgtacctgt
     5281 aaagcatgtc attccagaaa tcgttgcaca ttcgcattac actgtacaag gccaagactt
     5341 cccagcaggt aatggttcta gtgcatcaga ttactttaag ttatctaatg gtagtgacat
     5401 tgcagatgca actattacat gggtaagtgg acaagcgcca aataaagata atacacgtat
     5461 tggtgaagat ataactgtaa ctgcacatat cttaattgat ggcgaaacaa cgccgattac
     5521 gaaaacagca acatataaag tagtaagaac tgtaccgaaa catgtctttg aaacagccag
     5581 aggtgtttta tacccaggtg tttcagatat gtatgatgcg aaacaatatg ttaagccagt
     5641 aaataattct tggtcgacaa atgcgcaaca tatgaatttc caatttgttg gaacatatgg
     5701 tcctaacaaa gatgttgtag gcatatctac tcgtcttatt agagtgacat atgataatag
     5761 acaaacagaa gatttaacta ttttatctaa agttaaacct gacccaccta gaattgacgc
     5821 aaactctgtg acatataaag caggtcttac aaaccaagaa attaaagtta ataacgtatt
     5881 aaataactcg tcagtaaaat tatttaaagc agataataca ccattaaatg tcacaaatat
     5941 tactcatggt agcggtttta gttcggttgt gacagtaagt gacgcgttac caaatggcgg
     6001 aattaaagca aaatcttcaa tttcaatgaa caatgtgacg tatacgacgc aagacgaaca
     6061 tggtcaagtt gttacagtaa caagaaatga atctgttgat tcaaatgaca gtgcaacagt
     6121 aacagtgaca ccacaattac aagcaactac tgaaggcgct gtatttatta aaggtggcga
     6181 cggttttgat ttcggacacg tagaaagatt tattcaaaac ccgccacatg gggcaacggt
     6241 tgcatggcat gatagtccag atacatggaa gaatacagtc ggtaacactc ataaaactgc
     6301 ggttgtaaca ttacctaatg gtcaaggtac gcgtaatgtt gaagttccag tcaaagttta
     6361 tccagttgct aatgcaaagg cgccatcacg tgatgtgaaa ggtcaaaatt tgactaatgg
     6421 aacggatgcg atgaactaca ttacatttga tccaaataca aacacaaatg gtatcactgc
     6481 agcatgggca aatagacaac aaccaaataa ccaacaagca ggcgtgcaac atttaaatgt
     6541 cgatgtcaca tatccaggta tttcagctgc taaacgagtt cctgttactg ttaatgtata
     6601 tcaatttgaa ttccctcaaa ctacttatac gacaacggtt ggaggcactt tagcaagtgg
     6661 tacgcaagca tcaggatatg cacatatgca aaatgctact ggtttaccaa cagatggatt
     6721 tacgtataaa tggaatcgtg atactacagg tacaaatgac gcaaactggt cagctatgaa
     6781 taaaccgaat gtggctaaag tcgttaacgc aaaatatgac gtcatctata acggacatac
     6841 ttttgcaaca tctttaccag cgaaatttgt agtaaaagat gtgcaaccag cgaaaccaac
     6901 tgtgactgaa acagcggcag gagcgattac aattgcacct ggagcaaacc aaacagtgaa
     6961 tacacatgcc ggtaacgtaa cgacatacgc tgataaatta gttattaaac gtaatggtaa
     7021 cgttgtgacg acatttacac gtcgcaataa tacgagtcca tgggtgaaag aagcatctgc
     7081 agcaactgta gcaggtattg ctggaactaa taatggtatt actgttgcag caggtacttt
     7141 caaccctgct gatacaattc aagttgttgc aacgcaagga agcggagaga cagtgagtga
     7201 tgagcaacgt agtgatgatt tcacagttgt cgcaccacaa ccgaaccaag cgactactaa
     7261 gatttggcaa aatggtcata ttgatatcac gcctaataat ccatcaggac atttaattaa
     7321 tccaactcaa gcaatggata ttgcttacac tgaaaaagtg ggtaatggtg cagaacatag
     7381 taagacaatt aatgttgttc gtggtcaaaa taatcaatgg acaattgcga ataagcctga
     7441 ctatgtaacg ttagatgcac aaactggtaa agtgacgttc aatgccaata ctataaaacc
     7501 aaattcatca atcacaatta ctccgaaagc aggtacaggt cactcagtaa gtagtaatcc
     7561 aagtacatta actgcaccgg cagctcatac tgtcaacaca actgaaattg tgaaagatta
     7621 tggttcaaat gtaacagcag ctgaaattaa caatgcagtt caagttgcta ataaacgtac
     7681 tgcaacgatt aaaaatggca cagcaatgcc tactaattta gctggtggta gcacaacgac
     7741 gattcctgtg acagtaactt acaatgatgg tagtactgaa gaagtacaag agtccatttt
     7801 cacaaaagcg gataaacgtg agttaatcac agctaaaaat catttagatg atccagtaag
     7861 cactgaaggt aaaaagccag gtacaattac gcagtacaat aatgcaatgc ataatgcgca
     7921 acaacaaatc aatactgcga aaacagaagc acaacaagtg attaataatg agcgtgcaac
     7981 accacaacaa gtttctgacg cactaactaa agttcgtgca gcacaaacta agattgatca
     8041 agctaaagca ttacttcaaa ataaagaaga taatagccaa ttagtaacgt ctaaaaataa
     8101 cttacaaagt tctgtgaacc aagtaccatc aactgctggt atgacgcaac aaagtattga
     8161 taactataat gcgaagaagc gtgaagcaga aactgaaata actgcagctc aacgtgttat
     8221 tgacaatggc gatgcaactg cacaacaaat ttcagatgaa aaacatcgtg tcgataacgc
     8281 attaacagca ttaaaccaag cgaaacatga tttaactgca gatacacatg ccttagagca
     8341 agcagtgcaa caattgaatc gcacaggtac aacgactggt aagaagccgg caagtattac
     8401 tgcttacaat aattcgattc gtgcacttca aagtgactta acaagtgcta aaaatagcgc
     8461 taatgctatt attcaaaagc caataagaac agtacaagaa gtgcaatctg cgttaacaaa
     8521 tgtaaatcgt gtcaatgagc gattaacgca agcaattaat caattagtac ctttagctga
     8581 taatagtgct ttaaaaactg ctaagacgaa acttgatgaa gaaatcaata aatcagtaac
     8641 tactgatggt atgacacaat catcaatcca agcatatgaa aatgctaaac gtgcgggtca
     8701 aacagaatca acaaatgcac aaaatgttat taacaatggt gatgcgactg accaacaaat
     8761 tgccgcagaa aaaacaaaag tagaagaaaa atataatagc ttaaaacaag caattgctgg
     8821 attaactcca gacttggcac cattacaaac tgcaaaaact cagttgcaaa atgatattga
     8881 tcagccaacg agtacgactg gtatgacaag cgcatctatt gcagcattta atgaaaaact
     8941 ttcagcagct agaactaaaa ttcaagaaat tgatcgtgta ttagcctcac atccagatgt
     9001 tgcgacaata cgtcaaaacg tgacagcagc gaatgccgct aaatcagcac ttgatcaagc
     9061 acgtaatggc ttaacagtcg ataaagcgcc tttagaaaat gcgaaaaatc aactacaaca
     9121 tagtattgac acgcaaacaa gtacaactgg tatgacacaa gactctataa atgcatacaa
     9181 tgcgaagtta acagctgcac gtaataagat tcaacaaatc aatcaagtat tagcaggttc
     9241 accgactgta gaacaaatta atacaaatac gtctacagca aatcaagcta aatctgattt
     9301 agatcatgca cgtcaagctt taacaccaga taaagcgccg cttcaaactg cgaaaacgca
     9361 attagaacaa agcattaatc aaccaacgga tacaacaggt atgacgaccg cttcgttaaa
     9421 tgcgtacaac caaaaattac aagcagcgcg tcaaaagtta actgaaatta atcaagtgtt
     9481 gaatggcaac ccaactgtcc aaaatatcaa tgataaagtg acagaggcaa accaagctaa
     9541 ggatcaatta aatacagcac gtcaaggttt aacattagat agacagccag cgttaacaac
     9601 attacatggt gcatctaact taaaccaagc acaacaaaat aatttcacgc aacaaattaa
     9661 tgctgctcaa aatcatgctg cgcttgaaac aattaagtct aacattacgg ctttaaatac
     9721 tgcgatgacg aaattaaaag acagtgttgc ggataataat acaattaaat cagatcaaaa
     9781 ttacactgac gcaacaccag ctaataaaca agcgtatgat aatgcagtta atgcggctaa
     9841 aggtgtcatt ggagaaacga ctaatccaac gatggatgtt aacacagtga accaaaaagc
     9901 agcatctgtt aaatcgacga aagatgcttt agatggtcaa caaaacttac aacgtgcgaa
     9961 aacagaagca acaaatgcga ttacgcatgc aagtgattta aaccaagcac aaaagaatgc
    10021 attaacacaa caagtgaata gtgcacaaaa cgtgcaagca gtaaatgata ttaaacaaac
    10081 gactcaaagc ttaaatactg ctatgacagg tttaaaacgt ggcgttgcta atcataacca
    10141 agtcgtacaa agtgataatt atgtcaacgc agatactaat aagaaaaatg attacaacaa
    10201 tgcatacaac catgcgaatg acattattaa tggtaatgca caacatccag ttataacacc
    10261 aagtgatgtt aacaatgctt tatcaaatgt cacaagtaaa gaacatgcat tgaatggtga
    10321 agctaagtta aatgctgcga aacaagaagc gaatactgca ttaggtcatt taaacaattt
    10381 aaataatgca caacgtcaaa acttacaatc gcaaattaat ggtgcgcatc aaattgatgc
    10441 agttaataca attaagcaaa atgcaacaaa cttgaatagt gcaatgggta acttaagaca
    10501 agctgttgca gataaagatc aagtgaaacg tacagaagat tatgcggatg cagatacagc
    10561 taaacaaaat gcatataaca gtgcagtttc aagtgccgaa acaatcatta atcaaacaac
    10621 aaatccaacg atgtctgttg atgatgttaa tcgtgcaact tcagctgtta cttctaataa
    10681 aaatgcatta aatggttatg aaaaattagc acaatctaaa acagatgctg caagagcaat
    10741 tgatgcatta ccacatttaa ataatgcaca aaaagcagat gttaaatcta aaattaatgc
    10801 tgcatcaaat attgctggcg taaatactgt taaacaacaa ggtacagatt taaatacagc
    10861 gatgggtaac ttgcaaggtg caatcaatga tgaacaaacg acgcttaata gtcaaaacta
    10921 tcaagatgcg acacctagta agaaaacagc atacacaaat gcggtacaag ctgcgaaaga
    10981 tattttaaat aaatcaaatg gtcaaaataa aacgaaagat caagttactg aagcgatgaa
    11041 tcaagtgaat tctgctaaaa ataacttaga tggtacgcgt ttattagatc aagcgaagca
    11101 aacagcaaaa cagcagttaa ataatatgac gcatttaaca actgcacaaa aaacgaattt
    11161 aacaaaccaa attaatagtg gtactactgt cgctggtgtt caaacggttc aatcaaatgc
    11221 caatacatta gatcaagcca tgaatacgtt aagacaaagt attgccaaca aagatgcgac
    11281 taaagcaagt gaagattacg tagatgctaa taatgataag caaacagcat ataacaacgc
    11341 agtagctgct gctgaaacga ttattaatgc taatagtaat ccagaaatga atccaagtac
    11401 gattacacaa aaagcagagc aagtgaatag ttctaaaacg gcacttaacg gtgatgaaaa
    11461 cttagctgct gcaaaacaaa atgcgaaaac gtacttaaac acattgacaa gtattacaga
    11521 tgctcaaaag aacaatttga ttagtcaaat tactagtgcg acaagagtga gtggtgttga
    11581 tactgtaaaa caaaatgcgc aacatctaga ccaagctatg gctagcttac agaatggtat
    11641 taacaacgaa tctcaagtga aatcatctga gaaatatcgt gatgctgata caaataaaca
    11701 acaagagtat gataatgcta ttactgcagc gaaagcgatt ttaaataaat cgacaggtcc
    11761 aaacactgcg caaaatgcag ttgaagcagc attacaacgt gttaataatg cgaaagatgc
    11821 attgaatggt gatgcaaaat taattgcagc tcaaaacgca gcgaaacaac atttaggtac
    11881 tttaacgcat atcactacag ctcaacgtaa tgatttaaca aatcaaattt cacaagctac
    11941 aaacttagct ggtgttgaat ctgttaaaca aaatgcgaat agtttagatg gtgctatggg
    12001 taacttacaa acggctatca acgataagtc aggaacatta gcgagccaaa acttcttgga
    12061 tgctgatgag caaaaacgta atgcatacaa tcaagctgta tcagcagccg aaaccatttt
    12121 aaataaacaa actggaccga atacagcgaa aacagcagtc gaacaagcac ttaataatgt
    12181 taataatgcg aaacatgcat taaatggtac gcaaaactta aacaatgcga aacaagcagc
    12241 gattacagca atcaatggcg catctgattt aaatcaaaaa caaaaagatg cattaaaagc
    12301 acaagctaat ggtgctcaac gcgtatctaa tgcacaagat gtacagcaca atgcgactga
    12361 actgaacacg gcaatgggca cattaaaaca tgccatcgca gataagacga atacgttagc
    12421 aagcagtaaa tatgttaatg ccgatagcac taaacaaaat gcttacacaa ctaaagttac
    12481 caatgctgaa catattatta gcggtacgcc aacggttgtt acgacacctt cagaagtaac
    12541 agctgcagct aatcaagtaa acagcgcgaa acaagaatta aatggtgacg aaagattacg
    12601 tgaagcaaaa caaaacgcca atactgctat tgatgcatta acacaattaa atacacctca
    12661 aaaagctaaa ttaaaagaac aagtgggaca agccaataga ttagaagacg tacaaactgt
    12721 tcaaacaaat ggacaagcat tgaacaatgc aatgaaaggc ttaagagata gtattgctaa
    12781 cgaaacaaca gtcaaaacaa gtcaaaacta tacagacgca agtccgaata accaatcaac
    12841 atataatagc gctgtgtcaa atgcgaaagg tatcattaat caaactaaca atccgactat
    12901 ggatactagt gcgattaccc aagctacaac acaagtgaat aatgctaaaa atggtttaaa
    12961 cggtgctgaa aacttaagaa atgcacaaaa cactgctaag caaaacttaa atacattatc
    13021 acacttaaca aataaccaaa aatctgccat ctcatcacaa attgatcgtg caggtcatgt
    13081 gagtgaggta actgctacta aaaatgcagc aactgagttg aatacgcaaa tgggtaactt
    13141 ggaacaagct atccatgatc aaaacacagt taaacaaagt gttaaattta ctgatgcaga
    13201 taaagctaaa cgtgatgcgt atacaaatgc ggtaagcaga gctgaagcaa ttctgaataa
    13261 aacgcaaggt gcaaatacgt ctaaacaaga tgttgaagcg gctattcaaa atgtttcaag
    13321 tgctaaaaat gcattgaatg gtgatcaaaa cgttacaaat gcgaagaatg cagctaaaaa
    13381 tgcattaaat aacttaacgt caattaataa tgcacaaaaa cgtgacttaa caactaaaat
    13441 tgatcaagca acaactgtag ctggtgttga agctgtatct aatacgagta cacaattgaa
    13501 tacagcgatg gctaacttgc aaaatggtat taatgataaa acaaatacac tagcaagtga
    13561 aaactatcat gatgctgatt cagataagaa aactgcttat actcaagccg ttacgaacgc
    13621 agaaaatatt ttaaataaaa atagtggatc aaatttagac aaaactgccg ttgaaaacgc
    13681 gttgtcacaa gttgctaatg cgaaaggtgc cctaaatggt aaccataatt tagagcaagc
    13741 taaatcaaat gcaaacacta ctataaacgg acttcaacat ttaacaactg ctcaaaaaga
    13801 taaattgaaa caacaagtgc aacaagcaca aaatgttgca ggtgtagata ctgttaaatc
    13861 aagtgccaac acattaaatg gtgctatggg tacgttaaga aatagcatac aagataacac
    13921 agctacgaaa aatggccaaa actatcttga tgctacagaa cgtaacaaaa caaactataa
    13981 caatgctgtt gatagtgcta atggtgtcat taatgcaaca agcaatccaa atatggatgc
    14041 taatgcaatt aaccaaatcg ctacacaagt gacatcaacg aaaaatgcat tagatggtac
    14101 acataattta acgcaagcga aacaaacagc aacaaatgcc atcgatggtg ctactaactt
    14161 aaataaagcg caaaaagatg cgttaaaagc acaagttaca agtgcgcaac gtgttgcaaa
    14221 tgtaacaagt atccaacaaa ctgcaaatga acttaataca gctatgggtc aattacaaca
    14281 tggtattgat gatgaaaatg caacaaaaca aactcaaaaa tatcgtgacg ctgaacaaag
    14341 taagaaaact gcttatgatc aagctgtagc tgctgcgaaa gcaattttaa ataaacaaac
    14401 aggttcaaat tcagataaag cagcagttga ccgtgcatta caacaagtaa caagtacgaa
    14461 agatgcattg aatggtgatg caaaactggc agaagcgaaa gcggcagcta aacaaaactt
    14521 aggcacttta aaccatatta cgaatgcaca acgtactgac ttagaaggcc aaatcaatca
    14581 agcgacgact gttgatggcg ttaatactgt aaaaacaaat gccaatacat tagacggcgc
    14641 aatgaatagc ttacaaggtt caatcaatga taaagatgcg acattaagaa atcaaaatta
    14701 tcttgatgcg gatgaatcaa aacgaaatgc atatacgcaa gctgtcacag cggctgaagg
    14761 cattttaaat aaacaaactg gtggtaacac atctaaagca gacgttgata atgcattaaa
    14821 tgcagttaca agagcgaaag cggctttaaa tggtgctgac aacttaagaa atgcgaaaac
    14881 ttcagcaaca aatacgattg atggtttacc taacttaaca caattacaaa aagacaactt
    14941 gaagcatcaa gttgaacaag cgcaaaatgt agcaggtgta aatggtgtta aagataaagg
    15001 taatacgtta aatactgcca tgggtgcatt acgtacaagt atccaaaatg ataatacgac
    15061 gaaaacaagt caaaattatc ttgatgcatc tgacagcaac aaaaataatt acaatactgc
    15121 tgtaaataat gcaaatggtg ttattaatgc aacgaacaat ccaaatatgg atgctaatgc
    15181 gattaatggc atggcaaatc aagtcaatac aacaaaagca gcgttaaatg gtgcacaaaa
    15241 cttagctcaa gctaaaacaa atgcgacgaa cacaattaac aacgcacatg acttaaacca
    15301 aaaacaaaaa gatgcattaa aaacacaagt taacaatgca caacgtgtat ctgatgcaaa
    15361 taacgttcaa cacactgcaa ctgaattgaa cagtgcgatg acagcactta aagcagctat
    15421 tgctgataaa gaaagaacaa aagcaagcgg taattatgtc aatgctgatc aagaaaaacg
    15481 tcaagcgtat gattcaaaag tgactaacgc tgaaaatatc attagtggta caccgaatgc
    15541 gacattaaca gtcaatgacg taaatagtgc ggcatcacaa gtcaatgcgg ctaaaacagc
    15601 attaaatggt gataacaact tacgtgtagc gaaagagcat gccaacaata caattgacgg
    15661 cttagcacaa ttgaataatg cacaaaaagc aaaattaaaa gaacaagttc aaagtgcaac
    15721 tacattagat ggtgttcaaa ctgttaaaaa tagttctcaa acgttgaata cagcgatgaa
    15781 aggcttaaga gatagtattg cgaatgaagc aacaattaaa gcaggtcaaa actacactga
    15841 cgcaagtcca aataatcgta acgagtacga cagtgcagtt actgcagcaa aagcaatcat
    15901 taatcaaaca tcgaacccaa cgatggaacc aaatactatt acgcaagtaa catcacaagt
    15961 gacaactaaa gaacaggcat taaatggtgc gcgaaactta gctcaagcta agacaactgc
    16021 gaaaaacaac ttgaataact taacatcaat taacaatgca caaaaagatg cgttaacgcg
    16081 tagcattgat ggtgcaacaa cagtagctgg tgtaaatcaa gaaactgcaa aagcaacaga
    16141 attaaataac gcaatgcata gtttacaaaa tggtatcaat gatgagacac aaacaaaaca
    16201 aactcagaaa tacctagatg cagagccaag taagaaatca gcttatgatc aagcagtaaa
    16261 tgcagcgaaa gcaattttaa caaaagctag tggtcaaaat gtagacaaag cagcagttga
    16321 acaagcattg caaaatgtga acagtacgaa gacggcgttg aacggtgatg cgaaattaaa
    16381 tgaagctaaa gcagctgcga aacaaacgtt aggtacatta acacacatta ataatgcaca
    16441 acgtacagcg ttagacaatg aaattacaca agcaacaaat gttgaaggtg ttaatacagt
    16501 taaagccaaa gcgcaacaat tagatggtgc tatgggtcaa ttagaaacat caattcgtga
    16561 taaagacacg acgttacaaa gtcaaaatta tcaagatgct gatgatgcta aacgaactgc
    16621 ttattctcaa gcagtaaatg cagcagcaac tattttaaat aaaacagctg gcggtaatac
    16681 acctaaagca gatgttgaaa gagcaatgca agctgttaca caagcaaata ctgcattaaa
    16741 cggtattcaa aacttagatc gtgcgaaaca ggctgctaac acagcgatta caaatgcttc
    16801 ggacttaaat acaaaacaaa aagaagcatt aaaagcacaa gtaacaagtg caggacgtgt
    16861 atctgcagca aatggtgttg aacatactgc gactgaatta aatactgcga tgacagcttt
    16921 aaagcgtgcc attgctgata aagctgagac aaaagctagt ggtaactatg tcaatgctga
    16981 tgcgaataaa cgtcaagcat atgatgaaaa agttacagct gccgaaaata tcgttagtgg
    17041 tacaccaaca ccaacgttaa caccagcaga tgttacaaat gcagcaacgc aagtaacgaa
    17101 tgctaagacg cagttaaacg gtaatcataa tttagaagta gcgaaacaaa atgctaacac
    17161 tgcaattgat ggtttaactt ctttaaatgg tccgcaaaaa gcaaaactta aagaacaagt
    17221 gggtcaagcg acgacgttgc caaatgttca aactgttcgt gataatgcac aaacattaaa
    17281 cactgcaatg aaaggtctac gagatagcat tgcgaatgaa gcaacgatta aagcaggtca
    17341 aaactacaca gatgcaagtc aaaacaaaca aactgactac aacagtgcag tcactgcagc
    17401 aaaagcaatc attggtcaaa caactagtcc atcaatgaat gcgcaagaaa ttaatcaagc
    17461 gaaagaccaa gtgacagcta aacaacaagc gttaaacggt caagaaaact taagaactgc
    17521 gcaaacaaat gcgaagcaac atttgaacgg cttaagtgac ttaactgacg ctcaaaaaga
    17581 tgcagtgaaa cgtcaaatcg aaggtgcaac gcatgttaat gaagtaacac aagcacaaaa
    17641 taatgcggat gcattaaata cagctatgac gaacttgaaa aatggtattc aagatcagaa
    17701 tacgattaag caaggtgtta acttcactga tgccgacgaa gcgaaacgta atgcatatac
    17761 aaatgcagtg acgcaagctg aacaaatttt aaataaagca caaggtccaa atacttcaaa
    17821 agacggtgtc gaaactgcgt tagaaaatgt acaacgtgct aaaaacgaat tgaacggtaa
    17881 tcaaaatgtt gcgaacgcta agacaactgc gaaaaatgca ttgaataacc taacatcaat
    17941 taataatgca caaaaagaag cattgaaatc acaaattgaa ggtgcgacaa cagttgcagg
    18001 tgtaaatcaa gtgtctacaa cggcatctga attaaataca gcaatgagca acttacaaaa
    18061 tggtattaat gatgaagcag ctacaaaagc agctcagaaa tatactgatg cagatagaga
    18121 aaaacaaact gcatacaatg acgctgtaac agcagctaaa acgttattag ataaaacagc
    18181 tggttcaaat gacaataaag cagctgttga acaagcatta caacgtgtga atactgctaa
    18241 aacagcatta aatggtgacg agcgattaaa tgaagcgaag aacacagcta aacaacaagt
    18301 agcgacaatg tcacacttaa ctgatgctca aaaagcaaac ttaacatcgc aaatcgaaag
    18361 tggtacgact gttgcaggtg ttcaaggtat tcaagctaat gccggtactt tagatcaagc
    18421 aatgaatcaa ttaagacaaa gtattgcttc taaagatgcg actaaatcaa gcgaagatta
    18481 tcaagacgcg aatgcagatt tacaaaatgc atacaatgat gcggtaacta atgctgaagg
    18541 tattattagt gcaacgaata accctgaaat gaatcctgat acaattaacc aaaaagcgag
    18601 ccaagtgaac agtgcgaagt ctgcattgaa cggtgatgaa aaattagcag cagcaaaaca
    18661 aactgcgaaa tcagatatcg gtcgtttgac agacttgaac aatgcacaac gaactgcggc
    18721 aaatgctgaa gtggatcaag caccaaatct tgcagctgtc acagcggcta aaaataaagc
    18781 aacatcgtta aacacagcga tgggtaattt gaaacatgca cttgctgaaa aggataatac
    18841 gaaacgtagt gtcaattaca cagatgcgga tcaaccaaaa caacaagcgt atgatactgc
    18901 ggttacacaa gcagaagcaa ttactaatgc taatggcagc aacgcgaatg aaacacaagt
    18961 tcaagcagca ctaaaccaat tgaatcaagc taaaaatgac ttgaatggtg ataataaagt
    19021 tgctcaagca aaagagtcag cgaaacgtgc attagcttca tatagtaact tgaataatgc
    19081 gcaatcaact gcagcaatta gtcaaattga caatgcaacg acagtagcag gcgtaactgc
    19141 tgcacaaaat actgctaatg aattaaatac agcaatgggt caacttcaaa atggtataaa
    19201 tgaccaaaac actgttaaac aacaagtgaa ctttacagat gctgaccaag gtaagaaaga
    19261 tgcttacaca aatgctgtta cgaatgctca aggtatttta gataaagcac acggtcaaaa
    19321 tatgacgaaa gcacaagttg aagctgcatt aaatcaagta acgactgcta agaatgcttt
    19381 aaacggtgac gcaaatgtaa gacaagcaaa atcagatgcg aaagcaaact taggtacatt
    19441 aacacactta aataatgcac aaaaacaaga tttaacatca caaatcgaag gcgcaacaac
    19501 agtcaacggt gtaaatggtg ttaaaacgaa agcacaagac ttagatggtg caatgcaacg
    19561 attacaatca gctatagcaa ataaagatca aactaaagcg agcgaaaatt acatcgacgc
    19621 agatccaact aagaaaacag catttgataa tgctatcaca caagctgaat cttacttaaa
    19681 taaagatcac ggtgcgaata aagataagca agctgttgaa caagcaattc aaagtgtaac
    19741 gtctactgaa aatgctttga acggtgacgc gaacttacaa cgcgctaaaa ctgaagctat
    19801 acaagctatc gataacttga cacatttgaa tacaccacaa aaaacagcat taaaacaaca
    19861 agtgaacgct gcgcaacgtg tatcaggtgt aactgatctg aaaaatagtg ctacatcact
    19921 taataatgcg atggatcaat taaaacaagc aattgctgat catgacacaa ttgtagctag
    19981 tggtaattac actaacgcga gtcctgataa gcaaggtgct tatactgatg catataatgc
    20041 tgcgaaaaac attgtaaatg gttcgcctaa tgtgattaca aatgcagcag atgttacagc
    20101 agcaacacaa cgtgttaata atgctgaaac aggtttaaac ggtgatacaa acttagcaac
    20161 tgcgaagcaa caagctaaag atgcattacg tcaaatgaca catttatctg atgcacaaaa
    20221 acaaagtatt actggtcaaa ttgatagcgc gacacaagta actggcgttc aaagtgtgaa
    20281 agacaacgcg acaaatcttg ataatgcaat gaatcaactt cgaaatagta ttgcgaataa
    20341 agatgatgta aaagcgagtc aaccatatgt tgatgcagat agagataaac aaaatgcata
    20401 caatacagca gttacaaatg ctgaaaatat cattaatgca acgagtcagc cgacacttga
    20461 tccatctgca gtaacacaag cagctaatca agtgagcact aacaaaactg cgcttaatgg
    20521 tgcacaaaac ttagcgaata aaaagcaaga aacgactgct aacatcaacc aattaagtca
    20581 tttaaataat gctcaaaagc aagatttaaa tacgcaagtg acaaatgcac caaatattag
    20641 cacagtaaat caagtgaaaa ctaaagctga acaattagat caagcaatgg aacgtttaat
    20701 caacggaatc caagacaaag atcaagtgaa acaaagtgtt aactttacag atgcagatcc
    20761 agaaaaacaa acagcataca acaatgcggt aactgctgct gaaaatatta ttaatcaagc
    20821 aaatggtaca aatgcgaacc aatcacaagt tgaagcagca ctttcaactg taacaactac
    20881 gaaacaagcg ttgaatggtg atagaaaagt aacagatgct aaaaacaatg caaaccaaac
    20941 attatctacg ttagataact taaacaatgc acaaaaaggt gctgttactg gaaacatcaa
    21001 tcaagcgcac actgtagctg aagtaacgca agccattcaa accgctcagg aactgaatac
    21061 agcgatgggt aacttgaaaa atagcttgaa tgataaagac actacacttg gcagtcaaaa
    21121 ctttgcagat gcagatccag agaagaaaaa tgcatacaat gaagcggttc ataatgctga
    21181 aaatatttta aataaatcta caggtacgaa cgtgcctaaa gatcaagttg aagcagctat
    21241 gaatcaagtg aatgctacaa aagcagcgct taatggtact caaaaccttg aaaaagctaa
    21301 acaacacgca aatacagcaa ttgacggttt aagccattta acaaatgcac aaaaagaggc
    21361 attaaaacaa ttggtacaac aatcgactac tgttgcagaa gcacaaggta atgagcaaaa
    21421 agcaaacaat gttgatgcag caatggacaa attacgtcaa agtattgcag ataatgcgac
    21481 aacaaaacaa aaccaaaatt atactgatgc aagtcagaat aaaaaggatg cgtacaataa
    21541 tgctgtcaca actgcacaag gtattattga tcaaactaca agtccaactt tagatccgac
    21601 tgttatcaat caagctgctg gacaagtaag cacaactaaa aatgcattaa atggtaatga
    21661 aaacctagag gcagcgaaac aacaagcgtc acaatcatta ggttcattag ataacttaaa
    21721 taatgcgcaa aaacaaacag ttactgatca aattaatggc gcgcatactg ttgatgaagc
    21781 aaatcaaatt aagcaaaatg cgcaaaactt aaatacagcg atgggtaact tgaaacaagc
    21841 gatagctgac aaagatgcta cgaaagcgac agttaacttc actgatgcag atcaagcaaa
    21901 acaacaagca tataacactg ctgttacaaa tgctgaaaat atcatttcaa aagctaatgg
    21961 cggcaatgca acacaagctg aagttgaaca agcaatcaaa caagttaatg ctgcaaaaca
    22021 agcattaaat ggtaatgcca acgttcaaca tgcaaaagac gaagcaacag cattaattaa
    22081 tagctctaat gaccttaacc aagcacaaaa agacgcatta aaacaacaag ttcaaaatgc
    22141 aactactgta gctggtgtaa acaatgttaa acaaacagca caagagttaa acaatgctat
    22201 gacacaatta aaacaaggca ttgcagataa agaacaaaca aaagctgatg gtaactttgt
    22261 caatgcagat cctgataagc aaaatgcata taatcaagca gtagcgaaag ctgaagcatt
    22321 aattagtgct acgcctgatg ttgtcgttac acctagcgaa attactgcag cgttaaataa
    22381 agttacgcaa gctaaaaatg atttaaatgg taatacaaac ttagcaacgg cgaaacaaaa
    22441 tgttcaacat gctattgatc aattgccaaa cttaaaccaa gcgcaacgtg atgaatacag
    22501 caaacaaatc acgcaagcaa cacttgtacc aaacgtcaat gctattcaac aagcggcgac
    22561 aacgcttaat gacgcgatga cacaattgaa acaaggtatt gcgaataaag cacaaattaa
    22621 aggtagcgag aactatcacg atgctgatac tgacaagcaa acagcatatg ataatgcagt
    22681 aacaaaagca gaagaattgt taaaacaaac aacaaatcca acaatggatc caaatacaat
    22741 tcaacaagca ttaactaaag tgaatgacac aaatcaagca cttaacggta atcaaaaatt
    22801 agctgatgcc aaacaagatg ctaagacaac acttggtaca ctagatcatt taaatgatgc
    22861 tcaaaaacaa gcgctaacaa ctcaagttga acaagcacca gatattgcaa cagttaataa
    22921 tgttaagcaa aatgctcaaa atctgaataa tgctatgact aacttaaaca atgcattaca
    22981 agataaaact gagacattaa atagcattaa ctttactgat gcagatcaag ctaagaaaga
    23041 tgcttatact aatgcggttt cacatgcaga aggtatttta tctaaagcaa atggcagcaa
    23101 tgcaagtcaa actgaagtgg aacaagcgat gcaacgtgtg aacgaagcga aacaagcatt
    23161 gaatggtaat gacaatgtac aacgtgcaaa agatgcagcg aaacaagtga ttacaaatgc
    23221 aaatgattta aatcaagcgc aaaaagatgc attaaaacaa caagtcgatg ctgcgcaaac
    23281 tgttgcaaat gtaaacacga ttaagcaaac agcacaagat ttaaatcaag caatgacaca
    23341 attgaaacaa ggtattgcag ataaagacca aactaaagca aatggtaact ttgtcaatgc
    23401 tgatactgat aagcaaaatg cttacaacaa tgcggtagca catgctgaac aaataattag
    23461 tggtacacca aatgcaaacg tggatccaca acaagtggct caagcgttac aacaagtgaa
    23521 tcaagctaag ggtgatttaa acggtaacca taacttacaa gttgctaaag acaatgcaaa
    23581 tacagccatt gatcagttac caaacttaaa tcaaccacaa aaaacagcat taaaagacca
    23641 agtgtcgcat gcagaacttg ttacaggtgt taatgctatt aagcaaaatg ctgatgcgtt
    23701 aaataatgca atgggtacat tgaaacaaca aattcaagcg aacagtcaag taccacagtc
    23761 agttgacttt acacaagcgg atcaagacaa acaacaagca tataacaatg cggctaacca
    23821 agcgcaacaa atcgcaaatg gcataccaac acctgtattg acgcctgata cagtaacaca
    23881 agcagtgaca actatgaatc aagcgaaaga tgcattaaac ggtgatgaaa aattagcaca
    23941 agcgaaacaa gaagctttag caaatcttga tacgttacgc gatttaaatc aaccacaacg
    24001 tgatgcatta cgtaaccaaa tcaatcaagc acaagcgtta gctacagttg aacaaactaa
    24061 acaaaatgca caaaatgtga atacagcaat gagtaacttg aaacaaggta ttgcaaacaa
    24121 agatactgtc aaagcaagtg agaactatca tgatgctgat gccgataagc aaacagcata
    24181 tacaaatgca gtgtctcaag cggaaggtat tatcaatcaa acgacaaatc caacgcttaa
    24241 cccagatgaa ataacacgtg cattaactca agtgactgat gctaaaaatg gcttaaacgg
    24301 tgaagctaaa ttggcaactg aaaagcaaaa tgctaaagat gccgtaagtg ggatgacgca
    24361 tttaaacgat gctcaaaaac aagcattaaa aggtcaaatc gatcaatcgc ctgaaattgc
    24421 tacagtgaac caagttaaac aaacagcaac gagcctagat caagcaatgg atcaattatc
    24481 acaagctatt aatgataaag ctcaaacatt agcggacggt aattacttaa atgcagatcc
    24541 tgacaaacaa aatgcgtata aacaggcagt agcaaaagct gaagcattat tgaataaaca
    24601 aagtggtact aatgaagtac aagcacaagt tgaaagcatc actaatgaag tgaacgcagc
    24661 gaaacaagca ttaaatggta atgacaattt ggcaaatgca aaacaacaag caaaacaaca
    24721 attggcgaac ttaacacact taaatgatgc acaaaaacaa tcatttgaaa gtcaaattac
    24781 acaagcgcca cttgttacag atgtcactac gattaatcaa aaagcacaaa cgttagatca
    24841 tgcgatggaa ttattaagaa atagtgttgc ggataatcaa acgacattag cgtctgaaga
    24901 ttatcatgat gcaactgcgc aaagacaaaa tgactataac caagctgtaa cagctgctaa
    24961 taatatcatt aatcaaacta catcgcctac gatgaatcca gatgatgtta atggtgcaac
    25021 gacacaagtg aataatacga aagttgcatt agatggtgat gaaaaccttg cagcagctaa
    25081 acaacaagca aacaacagac ttgatcaatt agatcatttg aataatgcgc aaaagcaaca
    25141 gttacaatca caaattacgc aatcatctga tattgctgca gttaatggtc acaaacaaac
    25201 agcagaatct ttaaatactg cgatgggtaa cttaattaat gcgattgcag atcatcaagc
    25261 cgttgaacaa cgtggtaact tcatcaatgc tgatactgat aaacaaactg cttataatac
    25321 agcggtaaat gaagcagcag caatgattaa caaacaaact ggtcaaaatg cgaaccaaac
    25381 agaagtagaa caagctatta ctaaagttca aacaacactt caagcgttaa atggagacca
    25441 taatttacaa gttgctaaaa caaatgcgac gcaagcaatt gatgctttaa caagcttaaa
    25501 tgatcctcaa aaaacagcat taaaagacca agttacagct gcaactttag taactgcagt
    25561 tcatcaaatt gaacaaaatg cgaatacgct taaccaagca atgcatggtt taagacagag
    25621 cattcaagat aacgcagcaa ctaaagcaaa tagcaaatat atcaacgaag atcaaccaga
    25681 gcaacaaaac tatgatcaag ctgttcaagc cgcaaataat attatcaatg aacaaactgc
    25741 aacattagat aataatgcga ttaatcaagc agcgacaact gtgaatacaa cgaaagcagc
    25801 attacatggt gatgtgaagt tacaaaatga taaagatcat gctaagcaaa cggttagtca
    25861 attagcacat ctaaacaatg cacaaaaaca tatggaagat acgttaattg atagtgaaac
    25921 aactagaaca gcagttaagc aagatttgac tgaagcacaa gcattagatc aacttatgga
    25981 tgcattacaa caaagtattg ctgacaaaga tgcaacacgt gcgagcagtg catatgtcaa
    26041 tgcagaaccg aataaaaaac aatcctatga tgaagcagtt caaaatgctg agtctatcat
    26101 tgcaggatta aataatccaa ctatcaataa aggtaatgta tcaagtgcga ctcaagcagt
    26161 aatatcatct aaaaatgcat tagatggtgt tgaacgatta gctcaagata agcaaactgc
    26221 tggaaattct ctaaatcatt tagatcaatt aacaccagct caacaacaag cgctagaaaa
    26281 tcaaattaat aatgcaacaa ctcgtggcga agtagcacaa aaattaactg aagcacaagc
    26341 acttaaccaa gcaatggaag ctttacgtaa tagcattcaa gatcaacagc aaacggaagc
    26401 gggtagcaag tttatcaatg aagataaacc acaaaaagat gcttaccaag cagcagttca
    26461 aaatgcaaaa gatttaatta atcaaactaa caatccaacg cttgataaag cacaagttga
    26521 acaattgaca caagctgtta accaagctaa agataaccta cacggtgatc aaaaacttgc
    26581 agacgataaa caacatgcgg ttactgattt aaatcaatta aatggtttga ataatccgca
    26641 acgtcaagca cttgaaagcc aaataaacaa cgcagcaact cgtggcgaag tagcacaaaa
    26701 attagctgaa gcaaaagcgc ttgatcaagc aatgcaagca ttacgtaata gtattcaaga
    26761 tcaacaacaa acagaatctg gtagcaagtt tatcaatgaa gataaaccgc aaaaagatgc
    26821 ttaccaagca gcagttcaaa atgcaaaaga tttaattaac caaacaggta atccaacact
    26881 cgacaaatca caagtagaac aattgacaca agcagtaaca actgcaaaag ataatctaca
    26941 tggtgatcaa aaacttgctc gtgatcaaca acaagcagta acaactgtaa atgcattgcc
    27001 aaacttaaat catgcacaac aacaagcatt aactgatgct ataaatgcag cgcctacaag
    27061 aacagaggtt gcacaacatg ttcaaactgc tactgaactt gatcacgcga tggaaacatt
    27121 gaaaaataaa gttgatcaag tgaatacaga taaggctcaa ccaaattaca ctgaagcgtc
    27181 aactgataaa aaagaagcag tagatcaagc gttacaagct gcagaaagca ttacagatcc
    27241 aactaatggt tcaaatgcga ataaagacgc tgtagaccaa gtattaacta agcttcaaga
    27301 aaaagaaaat gagttaaatg gtaatgagag agtcgctgaa gctaaaacac aagcgaaaca
    27361 aactattgac caattaacac atttaaatgc tgatcaaatt gcaactgcta aacaaaacat
    27421 tgatcaagcg acgaaacttc aaccaattgc tgaattagta gatcaagcaa cgcaattgaa
    27481 tcaatctatg gatcaattac aacaagcagt taatgaacat gctaacgttg agcaaactgt
    27541 agattacaca caagcagatt cagataaaca aaatgcttat aaacaagcta ttgctgatgc
    27601 tgaaaatgta ttgaaacaaa atgcgaataa gcaacaagtg gatcaagcac ttcaaaatat
    27661 tttaaatgca aaacaagcat taaatggtga tgaacgtgta gcacttgcta aaacaaatgg
    27721 taaacatgac atcgaccaat tgaatgcatt aaacaatgct caacaagatg gatttaaagg
    27781 tcgcatcgat caatcaaacg atttaaatca aatccaacaa attgtagatg aggctaaggc
    27841 acttaatcgt gcaatggatc aattgtcaca agaaatcact gacaatgaag gacgcacgaa
    27901 aggtagcacg aactatgtca atgcagatac acaagtcaaa caagtatatg atgaaacggt
    27961 tgataaagcg aaacaagcac ttgataaatc gactggtcaa aacttaactg caaaacaagt
    28021 tatcaaatta aatgatgcag tcactgcagc taagaaagca ttaaatggtg aagaaagact
    28081 taataatcgt aaagctgaag cattacaaag attggatcaa ttaacacatc taaacaatgc
    28141 tcaaagacaa ttagcaatcc aacaaattaa taatgctgaa acgctaaata aagcatctcg
    28201 agcaattaat agagcaacta aattagataa tgcaatgggt gcagtacaac aatatattga
    28261 cgaacagcac cttggtgtta tcagcagcac aaattacatc aatgcagatg acaatttgaa
    28321 agcaaattat gataatgcaa ttgcgaatgc agcacatgag ttagataaag tgcaaggtaa
    28381 tgcaattgca aaagctgaag cagagcaatt gaaacaaaat attatcgatg ctcaaaatgc
    28441 attaaatgga gaccaaaacc ttgcaaatgc caaagataaa gcaaatgcgt ttgttaattc
    28501 gttaaatgga ttaaatcaac agcaacaaga tcttgcacat aaagcaatta acaatgccga
    28561 tactgtatca gatgtaacag atattgttaa taatcaaatt gacttaaatg atgcaatgga
    28621 aacattgaaa catttagttg acaatgaaat tccaaatgca gagcaaactg tcaattacca
    28681 aaacgctgac gataatgcta aaacaaactt cgatgatgcc aaacgtctag caaatacatt
    28741 gctaaatagt gataacacaa atgtgaatga tatcaatggc gcaatccaag cagtcaatga
    28801 tgcaatccat aatcttaatg gtgatcaacg actacaagat gctaaagaca aggcaattca
    28861 atctattaat caagctttag ctaataagct aaaagaaatc gaagcttcaa atgcgacgga
    28921 tcaagacaag cttattgcga aaaataaagc agaagaattg gcaaacagca tcatcaacaa
    28981 cattaataaa gcaacaagta atcaggctgt atctcaagtt caaacagcag gcaaccacgc
    29041 gattgaacaa gtgcatgcca atgaaatacc aaaagcaaaa attgatgcca ataaagacgt
    29101 tgataagcaa gttcaagcat taattgacga aattgatcga aatccaaatc taacagataa
    29161 ggaaaaacaa gcacttaaag atcgtattaa tcaaatactt caacaaggtc ataacggcat
    29221 taacaatgcg atgactaaag aagaaattga acaagccaaa gcacaacttg cgcaagcatt
    29281 acaagacatc aaagatttag tgaaagctaa agaagatgcg aaacaagatg ttgataaaca
    29341 agttcaagct ttaattgacg aaatcgatca aaatccaaat ctaacagata aggaaaaaca
    29401 agcacttaaa tatcgaatta atcaaatact tcaacaaggt cataacgaca ttaacaatgc
    29461 gctgactaaa gaagaaattg agcaggcaaa agcacaactt gcacaagcat tgcaagacat
    29521 caaagattta gtgaaagcta aagaagatgc gaaaaatgca ataaaagcct tagctaatgc
    29581 gaagcgtgat caaatcaatt caaatccaga tttaacacct gagcaaaaag caaaagcgct
    29641 caaagaaatt gacgaagctg aaaaacgagc actacaaaac gttgagaatg ctcaaactat
    29701 agatcaatta aatcgaggat taaacttagg tttagatgac attagaaata cacatgtatg
    29761 ggaggttgat gaacaacctg ctgtaaatga aatttttgaa gcaacacctg agcaaatcct
    29821 agttaatggt gaactcattg tacatcgtga tgacatcatt acagaacaag atattcttgc
    29881 acacataaac ttaattgatc agctttcagc agaagtcatc gatacaccat caactgcaac
    29941 gatttctgat agcttaacag caaaagttga agttacattg cttgatggat caaaagtgat
    30001 tgttaatgtt cctgtaaaag ttgtagaaaa agaattgtca gtagtcaaac aacaggcaat
    30061 tgaatcaatc gaaaatgcgg cacaacaaaa gattaatgaa atcaataata gtgtgacatt
    30121 aacactggaa caaaaagaag ctgcaattgc agaagttaat aagcttaaac aacaagcaat
    30181 tgatcatgtt aacaatgcac ctgatgttca ttcagttgaa gaaattcaac aacaagaaca
    30241 agcgcatatt gaacaattta atccagaaca atttacgatt gaacaagcaa aatcaaatgc
    30301 aattaaatcg attgaagatg caattcaaca tatgattgat gaaatcaaag ctcgtactga
    30361 tctaacagat aaagagaagc aagaagctat tgctaagtta aatcaattaa aagaacaagc
    30421 aattcaagcg attcaacgtg cgcaaagcat cgatgaaata agtgagcaat tggaacaatt
    30481 taaagctcaa atgaaagcag ctaatccaac agcaaaagaa ctagctaaac gcaagcaaga
    30541 agctattagt agaattaaag acttttcaaa tgaaaaaata aatagtattc gaaatagtga
    30601 aattggcaca gctgatgaaa aacaagcagc aatgaatcaa attaacgaaa ttgtgcttga
    30661 aacaattaga gatattaata atgcgcatac attacagcaa gttgaggctg cattgaacaa
    30721 tggtattgct cgaatttcag cagtacaaat tgtaacatct gatcgtgcta aacaatcgtc
    30781 aagtactgga aatgaatcta atagccattt aacaattggt tatggaactg caaatcatcc
    30841 atttaacagt tcgactattg gacataaaaa gaaacttgat gaagatgatg acattgatcc
    30901 acttcatatg cgtcacttta gtaataattt cggtaatgtt attaaaaacg ctattggtgt
    30961 ggtgggtatc tctggtttac tagctagttt ctggttcttc attgccaaac gtcgtcgtaa
    31021 agaagatgaa gaggaagaat tagaaataag agataataat aaagattcaa taaaagagac
    31081 tttagacgat acaaaacatt taccactttt atttgcgaaa cgtcgcagaa aagaagatga
    31141 agaagatgtt actgttgaag aaaaagattc gctaaataat ggcgagtcac tcgataaagt
    31201 taaacatacg ccgttcttct taccaaaacg tcgtcgtaaa gaagatgaag aagatgtgga
    31261 agttacaaat gaaaacacag atgaaaaagt gttgaaagat aacgaacatt caccactctt
    31321 attcgcaaaa cgacgcaaag ataaagagga agatgttgaa acaacaacta gtattgaatc
    31381 taaagatgag gacgttcctt tattattggc taaaaagaaa aatcaaaaag ataaccaatc
    31441 caaagacaaa aagtcagcat caaaaaatac ttctaaaaag gtagcagcta aaaagaagaa
    31501 aaagaaagct aagaaaaata aaaaataatt tgtttctttg ataaatagag gagcaccgat
    31561 tgacatcaca tcagtcggtg ctccttttat ttattctttt taattaattt atacaatgcc
    31621 tgttgagcgt gttgattcgc ttctttgttt tgttctctcg gtatccattt aacaaataat
    31681 aaatcaaaat ctttttcaaa tatttctatt tgatcaaaat aaggtttgaa atttgcgttt
    31741 ttcacataac cagcttcaat gctatctgca attagctttg agtctgtata taatagtgcg
    31801 ttttgaacat ttaattcacg tgcatgttct agtgcataaa tacatgcagc ccattctgca
    31861 gtgtggttat ccatttcgcc taactcatgt gtatatgtat aatgctgctc atcttctttg
    31921 attacaatgg cacatgtact tatgcctgga tttcctttcg tcgcagcatc aaaatttatt
    31981 ttcgccataa taaacctact ttctattcaa tacttagtta aagttactat tactgtaata
    32041 caaaatatgt tgggtaatcc attaaaaaac acgcatcact taaataagta acacgtgttt
    32101 aaaatactcg ctgattcaaa gatgattttc taatacgtag tactgtaata tacttcctaa
    32161 aaaaatcatc ttcaggctgg gacataaatc aatgttctat gctctacgat gttatattgg
    32221 cagtagttga ctgaacgaaa atgcgcttgt aacaagcttt tttcaattct agtcaggggc
    32281 cccaacacag agaatttcga aaagaaattc tacaggcaat gcgagttggg gtgtgggtcc
    32341 caacacagaa gatgacgaaa agtcagctta caataatgtg caagtttggg atgggcccca
    32401 acaaagagaa attggattcc caatttctac agacaatgca agttggggtg ggacgacgaa
    32461 ataaattttg cgaaaatatt atttctgtcc cactccctta aaacttattc ttttgtgtag
    32521 taagtgcgtt aatagccttg atctaactta tcaatcttac ctttacgata aaatgattta
    32581 gcaatatatc caaatggtac attgaaaact gttgaagcta attttaatac gtacgttgta
    32641 ataaatattt caaatacaac tgtaccaggt aaacttccga taaatgcgat agctacaaat
    32701 aaagctgtat caattattga gcttaaaaat gtactaccat atgcacggat gaaaaacgtt
    32761 ttatcagaac taaatacttt tttaattagt gaaaagataa atacatcaat atgttgacca
    32821 ataatatatg cgacgattga gcctaaagca atgcgtggca caacatcaaa gattgcgtgt
    32881 aatgcttttt gtgccatatc ttctggtgca ggaataaaat gtaatgacaa ttgcataaca
    32941 ataatcataa ttaatgttga tgaaaagcct aaccaaactg ctctttttgc aactctacgc
    33001 ccataaatat cgtttaatat atctgttgct aaataaatag aagcaaacat gacattacct
    33061 aaagttgctg aaataccaaa gatttctaca gttttaatca cttgtatgtt ggcaatgatt
    33121 gtgccaattg caacccatgc aattaaacct tgtttaccaa aaaagcgata cataagtacc
    33181 ataagcacga acgttgcaat aaacgtaact agtcctaaaa tttcattata catattaaaa
    33241 tgtcctccta aattttgatc atgcgggtgt ttagaaaccg ctcaataaat aactaactca
    33301 ttttactgaa aaatcgtttg cgatgcaata gcatttgaat ataaatacat tttacgtata
    33361 gaattgtaaa aggtttcatt caaatcttag ggtcaaaaat gttataatat ttttatgtca
    33421 aatttaaaac agtaacactt atttacaagg ttgcaatatt ttgaagtaat aaaggaagtg
    33481 tcgcgtattt taactttttc agagcaaaat gcactcgcga aaatagatga tttaatgaat
    33541 acttattgca atcaatgtcc aatcaaaact cgtctgcgta aattagaggg gaaaacgaag
    33601 gcgcatcatt tttgtatcaa tgagtgttca atagggaaag aaataaaaca attaggaaat
    33661 gaacttcaat aggaggaagt caaatgaaaa ttatatctat atcagaaaca ccgaaccaca
    33721 acacaatgaa gattacactt agtgaaagca gagaaggtat gacatcagat acgtatacta
    33781 aagttgatga ttcacagcca gcatttatta atgacatctt aaaggttgaa ggcgttaaat
    33841 caattttcca tgttatggac tttatttcag tagataaaga aaatgacgca aattgggaaa
    33901 cagtattgcc aaaagtagag gctgtattcg aataaatttt tcatcaacta gtattcgggg
    33961 ggaataaagt atatggaaat tttacgtata gagccaacac caagtccaaa tacaatgaaa
    34021 gttgttttgt catatacaag agaagacaag ttatctaata cttataaaaa agtagaagaa
    34081 acacaaccaa gatttataaa tcagttgtta tctatagatg gtatcacttc catttttcat
    34141 gtcatgaact tcttagctgt tgataaggca ccaaaagctg attgggaagt catattacct
    34201 gatattaaag ctgctttttc tgatgcgaat aaggttttag aatctgtaaa tgaacctcaa
    34261 attgacaatc attttggtga aattaaagct gaattattaa cttttaaggg tataccgtat
    34321 caaattaagc taacttctgc tgaccaagaa ttaagagaac aattaccaca aacatatgtt
    34381 gaccatatga ctcaagcgca aacagcacat gacaatattg tttttatgcg taaatggcta
    34441 gatttaggaa atcgctatgg aaatattcaa gaagtaatgg atggtgtcct agaagaagtg
    34501 ctaactacct atccagaatc acagttaccc gtattggtaa aacatgcttt agaagaaaat
    34561 cacgcaacta ataattatca tttctatcga catgtctctt tggatgaata tcatgcaact
    34621 gataattgga agactcgatt acgaatgtta aaccattttc caaagccgac ttttgaagat
    34681 ataccgctgc ttgatttagc tttatctgat gaaaaagtac cggttagacg tcaagcgatt
    34741 gtattattag gtatgattga aagtaaagaa attttaccgt atttatataa ggggcttcgt
    34801 gataaaagtc ctgttgtaag aagaacagca ggggattgca taagcgattt agggtatcca
    34861 gaggcactac cagaaatggt gctactatta gatgatccac agaaaatcgt taggtggcgt
    34921 gctgctatgt ttatctttga tgaaggtaat gcagagcagc ttcccgcact aaaagcccat
    34981 attaatgaca atgcgtttga agttaaatta caaattgaaa tggccatatc tcgtattgaa
    35041 aacggagatg aagcacttgg ttcagtatgg aaacaaatgg caaacagaac aatttaattg
    35101 gaggaattaa atatgaatgc atatgatgct tatatgaaag aaattgcgca acaaatgcgt
    35161 ggcgaattaa ctcaaaatgg ttttacaagt ttagaaacga gcgaagcagt atcggagtat
    35221 atgaaccaag taaatgctga tgacactact tttgtagtta ttaactctac atgcggctgt
    35281 gcagctggat tagcaagacc agctgcagta gcagttgcaa cacaaaatga acatagacct
    35341 acaaatacag ttacagtttt tgctgggcaa gataaagaag caactgctac aatgcgagaa
    35401 ttcattcagc aagcaccatc tagtccttcg tatgctttat tcaaaggtca agatttagtt
    35461 tattttatgc ctagagaatt tatcgaaggt agagatatta atgacattgc aatggactta
    35521 aaggatgcct ttgacgaaaa ttgtaaatag tacacataaa taaatataaa ggttaacaca
    35581 ttttataata ttaaaaatgg tgtctgtcat tgaaaataga gaatatagtt gtattctatt
    35641 tgttaaataa agtccgtttt taccaactat attttctaga aatttaactg ttttaatagg
    35701 acatcaaaca taatattcaa atcatgtgtt aacctctttt ttaaattttt ttagcattaa
    35761 agttatagat ttgggtaaac aattaccaat tggaaacata tatcacgtta cgatggggta
    35821 ggtacttaat cagcatttta taaataaagt aacggaattc atgatattaa tatcatattc
    35881 ctaaaatgag tgataacaaa atgctacata aagttaagtt atatcaaact aaatatacat
    35941 actataaata atgaaaatga ggtgttatcg catatgttga attcatttga tgcagcatat
    36001 cacagtcttt gtgaagaagt tttagaaata ggaaatacac gaaatgatcg cacaaataca
    36061 ggtacgattt cgaaatttgg tcatcaactt cgctttgact tatctaaagg atttccacta
    36121 ttaacgacaa agaaagtttc ttttaaatta gtagcaaccg aattattatg gttcattaaa
    36181 ggaggtacaa acatccaata cttattaaaa tataataata atatatggaa cgaatgggct
    36241 tttgaaaatt atatcaaatc agacgagtat aaaggtccag atatgacaga tttcgggcat
    36301 cgtgcattga gtgatcctga atttaacgaa caatataaag aacaaatgaa acaatttaag
    36361 caacgtattc ttgaagatga tacatttgcg aagcaattcg gggatttagg aaatgtttat
    36421 ggtaaacaat ggcgagattg ggttgataaa gatggtaatc attttgatca acttaaaaca
    36481 gtaattgaac aaattaagca taatccagat tcaaggcgac acatcgtatc tgcatggaat
    36541 ccaacagaaa ttgatacaat ggcacttccg ccttgtcata ccatgttcca gttttatgtc
    36601 caagatggta agttaagttg ccagttatac caacgtagcg cagatatctt tttaggtgtg
    36661 ccatttaata tcgcaagcta cgctttattg acacacctta ttgccaaaga atgtggactt
    36721 gaagtgggtg aatttgtgca tacatttgga gatgcacata tttattcaaa tcatattgat
    36781 gcgattcaaa cacaattagc acgtgaaagc ttcaatcctc caacattaaa aattaacagt
    36841 gacaagtcta ttttcgacat aaattatgaa gatttggaaa ttgttgacta tgaatcacat
    36901 ccagcaataa aagctccaat agcagtgtag tcattgcata gttagctaac catatagaca
    36961 tcaaaatgac atcatagtat tttcaagtgc aaaaaagtac ttttttgtgt taaacgtttt
    37021 cataaattat gcaaaatcat tatttctatc acactttatg ataaaaattg tgttaaatta
    37081 aagataactt agtaataaaa aatgaaatga tagaagaagg aggataatta tgactttatc
    37141 cattctagtt gcacatgact tgcaacgagt aattggtttt gaaaatcaat taccttggca
    37201 cctaccaaat gatttgaagc atgttaaaaa attatcaaca ggtcatactt tagtaatggg
    37261 tcgtaagaca tttgaatcga ttggtaaacc actaccgaat cgtcgaaatg ttgtacttac
    37321 ttcagataca agtttcaacg tagagggcgt tgatgtaatt cactctattg aagatattta
    37381 ccaactaccg ggccatgttt tcatatttgg agggcaaaca ttatttgaag aaatgattga
    37441 taaagtggac gacatgtata ttactgttat tgaaggtaaa ttccgtggtg atacgttctt
    37501 tccaccttat acatttgaag actgggaagt tgcctcttca gttgaaggta aactagatga
    37561 gaaaaataca attccacata cctttctaca tttaattcgt aaaaaataag ggggaaaacg
    37621 accatgacaa aacagattat agtaacagac tcaacatccg atttatctaa agaatactta
    37681 gaagcaaaca acattcatgt aattccttta agtttaacta ttgaaggagc ttcatacgtt
    37741 gaccaagtag atattacatc agaagaattt attaatcata ttgaaaatga tgaagatgta
    37801 aagacaagtc agccagccat aggtgaattt atatctgctt atgaagaact aggaaaagat
    37861 ggctctgaaa tcataagtat tcatctttct tcaggattaa gtggtacata taacactgct
    37921 taccaagcaa gtcaaatggt agatgctaat gtaactgtta ttgattcaaa atctatttct
    37981 tttggtttag ggtatcaaat acaacaccta gtagagcttg taaaagaagg tgtctcaact
    38041 tctgaaatag ttaaaaagtt aaatcattta agagaaaaca ttaaattatt tgtagttata
    38101 gggcaattga atcaattaat taaaggtggc agaattagta aaacaaaagg tttgattggt
    38161 aatcttatga aaattaaacc aattggtaca ctagatgatg gtcgcttaga gcttgtgcac
    38221 aatgcgagaa ctcaaaattc tagtatccaa tacttgaaaa aggaaattgc tgaatttata
    38281 ggagatcatg aaatcaaatc cattggtgtc gcacatgcta acgtcattga atatgttgat
    38341 aaattgaaga aagtttttaa tgaagctttt catgtgaata attacgatat aaatgtaact
    38401 acaccagtta tttctgcaca tactggtcaa ggtgcgattg gcctcgtagt ccttaagaag
    38461 taaatttaat cttttcagtg ttaattactt ccatttcaat cctttataga ctaaatttat
    38521 aattagatag atagaggagg taattcatat gacaaaagaa tatgcaacat tagcaggagg
    38581 atgtttctgg tgcatggtta aaccatttac atcatatcca ggcatcaagt cagtcgtatc
    38641 tggttatagt ggcggtcatg ttgacaaccc aacttatgaa caggtatgta cgaatcaaac
    38701 cggccatgtc gaagcagtac aaattacgtt tgatccagag gttacttcct ttgaaaatat
    38761 attagacata tatttcaaaa catttgaccc aactgatgat caagggcaat ttttcgatag
    38821 aggcgaaagc tatcaaccag tcattttcta tcatgatgaa catcagaaaa aggctgctga
    38881 gtttaaaaag caacaattaa atgaacaagg tattttcaag aaaccagtga ttacacctat
    38941 taaaccatat aaaaatttct atccagctga agactaccat caagattatt acaaaaagaa
    39001 cccggtacat tattaccaat atcaacgtgg ttcaggtaga aaagcgttta tagaatcaca
    39061 ttgggggaat caaaatgctt aaaaaagata aaagtgaact aacagatata gaatatattg
    39121 ttacacaaga aaacggcact gaaccaccat ttatgaatga atattggaat cattttgcta
    39181 aaggaattta tgtagataaa atttctggta aacctttatt tacatctgaa gaaaagtttc
    39241 attctgaatg tggatggcct agcttttcca aagcgcttga tgacgatgaa attatagaat
    39301 tagtcgacaa atcatttggt atgttgagaa ctgaagtgcg ttcagaagaa tcaaatagtc
    39361 atttaggaca tgtctttaat gatggaccta aagaaagtgg cggcttaaga tactgtatca
    39421 attccgctgc aattcaattt attccatatg aaaagttaga agaattgggt tatggcgatt
    39481 taatatcaca ttttgataag taggagtgga aagtatgttt aaaaaattat tcggaaaagg
    39541 caaagaagtt cagaaagata tcgcaattta tgcaccatta actggagaat tcgtgaaaat
    39601 tgaagatatt ccagatcctg tattcgcaca aaaaatgatg ggcgaaggtt ttggtattaa
    39661 tccaactgaa ggagaagttg tgtctccaat tgcaggacgt gttgacaatg tctttccaac
    39721 taagcatgcg attgggttaa aagcagataa cggattagaa ttattagttc atatcggttt
    39781 agacacagtt caattagacg gtgaaggatt tgaagtgtta gtgtctagtg gtgacgaagt
    39841 taacgtcgga gatccattag taagattcaa ccttgaatat attaataata acgctaaatc
    39901 tgtaatttca ccaattataa ttactaacac tgatcaagca gcttcaatta atatttatga
    39961 tgaaaatgct gtgattaaag gtgaaacaaa agtgattgat gtgacaatga actaatgaaa
    40021 aactattcgt tttatcaatt tgtcatgaca gttcgtggtc gacacgacga taaaggtcgt
    40081 ctagcagaag agatatttga cgatcttgct ttcccaaaac acgatgatga ttttaacata
    40141 ctgtctgatt atattgagac acatggtgat ttcacattgc caatgtctgt atttgatgat
    40201 ttatatgaag aatatacgga atggctaaaa ttttaatata atttttaata ataatagtta
    40261 gaaccagggt gatgcaattc gttatcctgg ttttaattta aaataaacta agttgtgact
    40321 aaaaattaat caattatagt gaaatatggt gcgctatctt gcataaattg atatgattaa
    40381 ctacacagaa tttaaaagta cataatacat aataaggaag tgatacaatg gatgataagc
    40441 aacacacatc ttcatccgat gatgaacgcg ctgaaattgc aacaagcaat caagaccaag
    40501 aaactaattc atcgaaacgc gttcacttaa aacgttggca attcatatca atattaattg
    40561 gtacaatcct aataacagct gtcatcacag ttgttgcata tatttttata aatcaaaaaa
    40621 taagtggttt aaacaaaact gatcaagcaa acttaaataa aattgaaaat gtgtataaaa
    40681 tcttaaatag tgattattac aaaaaacagg actctgacaa gttaagtaaa gctgcaattg
    40741 atggcatggt caaagaatta aaagatcctt attctgaata tttaacaaaa gaacaaacga
    40801 aatcctttaa tgaaggtgtt tcaggtgatt ttgtaggtat tggtgcagaa atgcaaaaga
    40861 aaaatgatca aattatggtt actagtccta tgaagggatc tccagcagaa cgtgctggca
    40921 ttcgtcctaa agatgtcatt actaaagtaa atggaaaatc aattaaaggt aaagcattag
    40981 atgaagttgt caaagatgtt cgtggtaaag aaaacactga agtcacttta actgttcaac
    41041 gaggtagtga agaaaaagac gttaagatta aacgtgaaaa aattcatgtt aaaagtgttg
    41101 agtataagaa aaaaggtaaa gttggagtta ttactattaa taaattccag aatgatacat
    41161 caggtgaatt gaaagatgca gttctaaaag ctcacaaaga tggtttgaaa aagattgttt
    41221 tagatttaag aaataatcca ggtggactac tagatgaagc tgttaaaatg gcaaatattt
    41281 ttatcgataa aggaaaaact gttgttaaac tagaaaaagg taaagatact gaagcaattc
    41341 aaacttctaa tgatgcgtta aaagaagcga aagacatgga tatatccatc ttagtgaatg
    41401 aaggttcagc tagcgcttct gaagtgttta ctggtgcgct aaaagactat aataaagcta
    41461 aagtttatgg gtcaaaaaca ttcggcaaag gtgtcgtaca aactacaaga gagtttaagg
    41521 atggttcatt gttaaaatat actgaaatga aatggttaac gccagatggt cattatattc
    41581 acggtaaagg cattaaacca gacgttacta ttgacacacc taaatatcaa tctttaaatg
    41641 tcattcctaa tacgaaaaca tttaaagttg gagacgatga taaaaatatt aaaactatta
    41701 aaattggttt atcagcttta ggttataaag ttgataatga atcaacgcaa tttgataaag
    41761 ctttagaaaa tcaagttaaa gctttccaac aagcgaataa acttgaggta actggtgaat
    41821 ttaataaaga aacgaataat aaatttactg agttattagt tgaaaaagct aataaacatg
    41881 atgatgttct cgataagttg attaatattt taaaataagc gatacacact actaaaattg
    41941 tattattatt atgttaatga cacgcctcct aaatttgcaa agatagcaat ttaggaggcg
    42001 tgtttatttt tattgacgtc taactctaaa agatataaat tagacattta caaatgatgt
    42061 aaataacgca atttctatca tcgctgataa caattcatgg tttaatatgc aatgagcata
    42121 tactttttaa atagtattat tcactagttt taacaatcaa ttaattggta tatgatactt
    42181 ttattggtta tttttatccc atagtgtgat aattactatt tttcattcat aataaaggtt
    42241 taaagcatgt taatagtgtg taagattaac atgtactgaa aaacatgttt aacataatga
    42301 tataaggagt gacgtacatg atccgtctag gtaaaatgtc agatttagat caaatcttaa
    42361 atctagtaga agaagcaaaa gaattaatga aagaacacga caacgagcaa tgggacgatc
    42421 agtacccact tttagaacat tttgaagaag atattgctaa agattatttg tacgtattag
    42481 aggaaaatga caaaatttat ggctttattg ttgtcgacca agaccaagca gaatggtatg
    42541 atgacattga ctggccagta aatagagaag gcgcctttgt tattcatcga ttaactggtt
    42601 cgaaagaata taaaggagct gctacagaat tattcaatta tgttattgat gtagttaaag
    42661 cacgtggtgc agaagttatt ttaacggaca cctttgcgtt aaacaaacct gcacaaggtt
    42721 tatttgccaa atttggattt cataaggtcg gtgaacaatt aatggaatat ccgccatatg
    42781 ataaaggtga accattttat gcatattata aaaatttaaa agaatagagg taatattaat
    42841 gacgaaaatc gcatttaccg gagggggaac agttggacac gtatcagtaa atttaagttt
    42901 aattccaact gcattatcac aaggttatga agcgctttat attggttcta aaaatggtat
    42961 tgaaagagaa atgattgaat cacaactacc agaaattaag tattatccta tttcgagtgg
    43021 taaattaaga agatatattt ctttagaaaa tgccaaagac gtatttaaag tattgaaagg
    43081 tattcttgat gctcgtaaag ttttgaaaaa agaaaaacct gatctattat tttcaaaagg
    43141 tggatttgta tctgtgcctg ttgttattgc agccaaatca ttaaatatac caactcttat
    43201 tcatgaatct gacttaacac caggattagc gaataagata gcacttaaat ttgccaagaa
    43261 aatatataca acatttgaag aaacgctaaa ctacttacct aaagagaaag ctgattttat
    43321 tggagcaaca attcgagaag atttaaaaaa tggtaatgca cataatggtt atcaattaac
    43381 aggctttaat gaaaataaaa aagttttact cgttatgggt ggaagcttag gaagtaaaaa
    43441 attaaatagc attattcgcg aaaacttaga tgcattatta caacaatatc aagtgataca
    43501 tttaactggt aaaggattaa aagatgctca agttaaaaaa tcaggatata tacaatatga
    43561 atttgttaaa gaggatttaa cagatttatt agcaattacg gatacagtaa taagtagagc
    43621 tggatcaaat gcgatttatg agttcttaac attacgtata ccaatgttat tagtaccatt
    43681 aggtttagat caatcccgag gcgaccaaat tgacaatgca aatcattttg ctgataaagg
    43741 atatgctaaa gcgattgatg aagaacaatt aacagcacaa attttattac aagaactaaa
    43801 tgaaatggaa caggaaagaa ctcgaattat caataatatg aaatcgtatg aacaaagtta
    43861 tacgaaagaa gctttatttg ataagatgat taaagacgca ttgaattaat ggggggtaat
    43921 gctttatgag tcaatggaaa cgtatctctt tgctcatcgt ttttacattg gtttttggaa
    43981 ttatcgcgtt tttccacgaa tcaagacttg ggaaatggat tgataatgaa gtttatgagt
    44041 ttgtatattc atcagagagc tttattacga catctatcat gcttggggct actaaagtag
    44101 gtgaagtctg ggcaatgtta tgtatttcat tacttcttgt ggcatatctc atgttaaagc
    44161 gccacaaaat tgaagcatta ttttttgcat taacaatggc attatctgga attttgaatc
    44221 cagcattaaa aaatatattc gatagagaaa gacctacatt gctgcgttta attgatataa
    44281 caggatttag ttttcctagc ggtcatgcta tgggatcaac tgcatatttt ggaagtggta
    44341 tctatctatt aaatcgatta aatcaaggta attcaaaagg tattcttata gggttatgtg
    44401 cagctatgat tttattgatt tccatatcac gtgtatatct aggtgtacat tatccaacag
    44461 atattattgc cggcattatt ggtggattat tttgcattat tttatcaacg ttattactta
    44521 gaaataaatt aataaattaa atagt
//
LOCUS       DAMQYT010000005        42775 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMQYT010000005 DAMQYT010000000
VERSION     DAMQYT010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 42775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42775
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.005"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..673
                     /gene="pyrF"
                     /locus_tag="PGA33_000196"
     CDS             <1..673
                     /gene="pyrF"
                     /locus_tag="PGA33_000196"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDG6472037.1"
                     /translation="ALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQLINEIKER
                     GHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLRKHNQDTK
                     IIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLESRMLTEKL
                     GTSFLKVTPGIRPKGASQNDQHRITTPEEARQLGSTHIVVGRPITQSDNPVESYHKIK
                     ESWLV"
     gene            673..1284
                     /locus_tag="PGA33_000197"
     CDS             673..1284
                     /locus_tag="PGA33_000197"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDG6472038.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKLAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            1314..1526
                     /locus_tag="PGA33_000198"
     CDS             1314..1526
                     /locus_tag="PGA33_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472039.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQEQI"
     gene            1963..2364
                     /locus_tag="PGA33_000199"
     CDS             1963..2364
                     /locus_tag="PGA33_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDG6472040.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(2627..4324)
                     /locus_tag="PGA33_000200"
     CDS             complement(2627..4324)
                     /locus_tag="PGA33_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDG6472041.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFITS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLYKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS"
     gene            4599..5222
                     /gene="gmk"
                     /locus_tag="PGA33_000201"
     CDS             4599..5222
                     /gene="gmk"
                     /locus_tag="PGA33_000201"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDG6472042.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLEHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            5222..5440
                     /locus_tag="PGA33_000202"
     CDS             5222..5440
                     /locus_tag="PGA33_000202"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDG6472043.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            5656..6855
                     /gene="coaBC"
                     /locus_tag="PGA33_000203"
     CDS             5656..6855
                     /gene="coaBC"
                     /locus_tag="PGA33_000203"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDG6472044.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            6855..9263
                     /gene="priA"
                     /locus_tag="PGA33_000204"
     CDS             6855..9263
                     /gene="priA"
                     /locus_tag="PGA33_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDG6472045.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMVASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
     gene            9769..10722
                     /locus_tag="PGA33_000205"
     CDS             9769..10722
                     /locus_tag="PGA33_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472046.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(10885..11088)
                     /locus_tag="PGA33_000206"
     CDS             complement(10885..11088)
                     /locus_tag="PGA33_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HDG6472047.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            11387..11875
                     /locus_tag="PGA33_000207"
     CDS             11387..11875
                     /locus_tag="PGA33_000207"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDG6472048.1"
                     /translation="MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            11868..12803
                     /locus_tag="PGA33_000208"
     CDS             11868..12803
                     /locus_tag="PGA33_000208"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDG6472049.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPNLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            12800..14107
                     /gene="rsmB"
                     /locus_tag="PGA33_000209"
     CDS             12800..14107
                     /gene="rsmB"
                     /locus_tag="PGA33_000209"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDG6472050.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            14110..15204
                     /gene="rlmN"
                     /locus_tag="PGA33_000210"
     CDS             14110..15204
                     /gene="rlmN"
                     /locus_tag="PGA33_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDG6472051.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            15211..15954
                     /locus_tag="PGA33_000211"
     CDS             15211..15954
                     /locus_tag="PGA33_000211"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HDG6472052.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEQHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            15951..17945
                     /gene="pknB"
                     /locus_tag="PGA33_000212"
     CDS             15951..17945
                     /gene="pknB"
                     /locus_tag="PGA33_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HDG6472053.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
     gene            18173..19048
                     /gene="rsgA"
                     /locus_tag="PGA33_000213"
     CDS             18173..19048
                     /gene="rsgA"
                     /locus_tag="PGA33_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDG6472054.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERENELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARILVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            19049..19693
                     /locus_tag="PGA33_000214"
     CDS             19049..19693
                     /locus_tag="PGA33_000214"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDG6472055.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            19700..20341
                     /locus_tag="PGA33_000215"
     CDS             19700..20341
                     /locus_tag="PGA33_000215"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDG6472056.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(20722..20910)
                     /locus_tag="PGA33_000216"
     CDS             complement(20722..20910)
                     /locus_tag="PGA33_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDG6472057.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
     gene            21353..21727
                     /locus_tag="PGA33_000217"
     CDS             21353..21727
                     /locus_tag="PGA33_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDG6472058.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
     gene            21742..23388
                     /gene="fakA"
                     /locus_tag="PGA33_000218"
     CDS             21742..23388
                     /gene="fakA"
                     /locus_tag="PGA33_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HDG6472059.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            23578..25638
                     /gene="recG"
                     /locus_tag="PGA33_000219"
     CDS             23578..25638
                     /gene="recG"
                     /locus_tag="PGA33_000219"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDG6472060.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDIDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEINVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            25841..26413
                     /gene="fapR"
                     /locus_tag="PGA33_000220"
     CDS             25841..26413
                     /gene="fapR"
                     /locus_tag="PGA33_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDG6472061.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            26418..27404
                     /gene="plsX"
                     /locus_tag="PGA33_000221"
     CDS             26418..27404
                     /gene="plsX"
                     /locus_tag="PGA33_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDG6472062.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNHDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            27397..28323
                     /gene="fabD"
                     /locus_tag="PGA33_000222"
     CDS             27397..28323
                     /gene="fabD"
                     /locus_tag="PGA33_000222"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDG6472063.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAANTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            28316..29050
                     /gene="fabG"
                     /locus_tag="PGA33_000223"
     CDS             28316..29050
                     /gene="fabG"
                     /locus_tag="PGA33_000223"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDG6472064.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            29293..29526
                     /locus_tag="PGA33_000224"
     CDS             29293..29526
                     /locus_tag="PGA33_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDG6472065.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            29642..30373
                     /locus_tag="PGA33_000225"
     CDS             29642..30373
                     /locus_tag="PGA33_000225"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDG6472066.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            30520..34086
                     /gene="smc"
                     /locus_tag="PGA33_000226"
     CDS             30520..34086
                     /gene="smc"
                     /locus_tag="PGA33_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDG6472067.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIYGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETYLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQNQQTKHQLKDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEAKLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            34086..35336
                     /gene="ftsY"
                     /locus_tag="PGA33_000227"
     CDS             34086..35336
                     /gene="ftsY"
                     /locus_tag="PGA33_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDG6472068.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDA
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGSK"
     gene            35323..35655
                     /locus_tag="PGA33_000228"
     CDS             35323..35655
                     /locus_tag="PGA33_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDG6472069.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            35681..37048
                     /gene="ffh"
                     /locus_tag="PGA33_000229"
     CDS             35681..37048
                     /gene="ffh"
                     /locus_tag="PGA33_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDG6472070.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTQLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            37483..37758
                     /gene="rpsP"
                     /locus_tag="PGA33_000230"
     CDS             37483..37758
                     /gene="rpsP"
                     /locus_tag="PGA33_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDG6472071.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            37946..38449
                     /gene="rimM"
                     /locus_tag="PGA33_000231"
     CDS             37946..38449
                     /gene="rimM"
                     /locus_tag="PGA33_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDG6472072.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            38449..39186
                     /gene="trmD"
                     /locus_tag="PGA33_000232"
     CDS             38449..39186
                     /gene="trmD"
                     /locus_tag="PGA33_000232"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDG6472073.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            39289..39639
                     /gene="rplS"
                     /locus_tag="PGA33_000233"
     CDS             39289..39639
                     /gene="rplS"
                     /locus_tag="PGA33_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDG6472074.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(39883..42489)
                     /gene="yfhO"
                     /locus_tag="PGA33_000234"
     CDS             complement(39883..42489)
                     /gene="yfhO"
                     /locus_tag="PGA33_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HDG6472075.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLRSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSANMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWILF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVCI
                     IAILYVLLSPTHPLALIVGIILLIVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGSFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYGFKIKSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNIKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRVVTPVTIRIKAPDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKHGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWARQK"
BASE COUNT        15735 a         5859 c         8058 g        13123 t
ORIGIN      
        1 tgcattagat tttgaatcaa aagaaaaagt aaatcaattt ttagatttat ttgatgaatc
       61 attattcgta aaagtaggta tggaactttt ttatcaagaa ggtcctcaat taattaatga
      121 gataaaagaa agaggccatg atgtattttt agatttaaaa ctgcatgata ttcctaatac
      181 agttggtaag gcgatggaag gactagctaa attgaatgtt gatctggtaa atgttcatgc
      241 tgctggtggc gtaaaaatga tgtctgaggc cattaaagga ttaagaaaac ataatcaaga
      301 tacaaaaatt attgcagtaa cacagcttac gtcaacaaca gaagacatgt tacgacacga
      361 acaaaatata caaacatcga ttgaagaggc cgttttaaat tatgccaagt tagcaaatgc
      421 agctggttta gatggcgttg tttgttcacc tcttgaaagt cgtatgttga ctgaaaagtt
      481 aggtacatca tttttaaaag taacaccagg tattagacct aaaggtgcat ctcaaaatga
      541 ccaacaccgt attacgacac cggaagaagc aagacagctt ggttcgacgc atattgtagt
      601 cggtagaccg attacacaaa gtgacaatcc agtcgaaagt tatcataaaa ttaaagaaag
      661 ttggttagta taatggctaa agaaattgca aaatcattat tagatattga agctgtaaca
      721 ttatcaccaa atgatttata tacatggagt tcaggtatta aatcaccgat ttactgtgat
      781 aaccgtgtta cgttaggtta tcctttagtt cgaggcgcaa tccgcgatgg tttaattaac
      841 ttaattaaag aacactttcc tgaagtagaa gttatttctg gtactgcaac agctggtatt
      901 ccacatgcag cttttattgc tgaaaaatta aaattaccaa tgaattatgt tcgttcatca
      961 aataagagtc atggtaagca aaatcaaatc gaaggtgcta aaagtgaagg taaaaaagta
     1021 gttgtgatag aagatttaat ttcgacaggg ggatcttcag tcacagcagt tgaagcctta
     1081 aaactagcag gtgcagaagt attaggtgtt gtagctatct ttacttacgg tttgaaaaaa
     1141 gcagatgata catttagcaa tattcaacta cctttttaca ctttaagtga ttacaatgaa
     1201 ttaattgaag tagctgaaaa tgaaggtaaa atttctagtg aagatatcca aacattagtt
     1261 gaatggagag acaacttagc ataatataga cactagaagg aggaattcaa caaatgaatg
     1321 acaaaacatc taatgattta tatgggaaga taaaacattg taacgaattt atcaatcatt
     1381 caaatgattc caatctatct agtagtcacg atgtcgacga aagttcaacg aagcaaaaac
     1441 atataaaaaa taaaacaact atagatcata atgatgattt atttaaacat gtaaaggata
     1501 tattacgtaa acaagaacaa atttaaaaac aaaaaagcta tagtgtttaa tatcaggggc
     1561 tgcttaaccg catccgaata ttacaaacac tatagctttt attatataaa aaatttatta
     1621 gcggataatt aactttatta aaatccaacc aattaagaat acgagtaata ggactaatac
     1681 tggaatcaca taatgtaaca taacgtccct cctttaactt aattttaatt gtaatcaaat
     1741 ttgacaataa gtcaaaacat taatacctat gataagtatc atttattaac atatgtatca
     1801 tatttttaat cttgcgtaat ttttatcgtt aactatgtga tttaatcaac aggccaataa
     1861 gacgtttgtc attcgtgcac ataacggtac gacgcgcttt tgtcatttta attatgttaa
     1921 gataatagta gttatagaag ttcaatctat aggaggcata gcatggatat tccaaaaatc
     1981 acgacatttt taatgtttaa taaccaagct gaagaagctg ttaaactata cacaagctta
     2041 tttgaagata gtgagattat aacaatggct aagtatggtg aaaatggacc tggtgatccc
     2101 gggactgtac aacactcaat atttacatta aatggacaag tattcatggc gattgatgct
     2161 aatagtggca cagaattacc aatgaatcct gcgatttcat tatttgttac agtaaaagat
     2221 actattgaaa tggaacgact atttaatgga ttaaaagatg aaggtgccat tttaatgcca
     2281 aaaacgaata tgccaccata cagagagttt gcttgggttc aagataagtt tggagtaagt
     2341 tttcaattag cattacctga gtaaaaggat tcgcacagcg ttgaatgata aagaaacact
     2401 ttttcttatg catgcgtttt acctatgtat ttctatttta agtacacatt agcattttag
     2461 tttcactcat ttttaaaatc acaaagataa tttgtaactt aaatgatgat acgttattta
     2521 aaaacacgat acttcgtttc aatgaacgca ttaaataata aataaacacc tcaccataag
     2581 aaaggactac tttcttttgt gaggtgttta tttgtcgaca acttgattat gattgtttca
     2641 ttttttgaat aagttcataa tcaggtgtag catacaaagt tttttgattg tcatatgtta
     2701 caaacccagg ctttgcacct gatggtttat gcacattttt aattaatgta taatcaacag
     2761 gtatttgtcc agaattacca gcttttgaaa agtatcctgc taacatagcc gcttccttga
     2821 ttgtcgtatc acttggtgca tcattaaata taacgacatg tgaaccagga atatcttttg
     2881 tgtgtaacca tgtgtgagtt tttttagctt ttttatttgt taaataatca ttttgcttgt
     2941 tattcttacc aacatatata tcgtcgccat cagttgatac ataatgttgt aattgaatct
     3001 gcgctttctt tttcttagtt tgatttttac gctgtttcat aaagccttgt tctgctaatt
     3061 catctctaat ttcatcaatg tcatggacag aaatatgatg taattgttgt tcgattgttg
     3121 aaaaataatc tatattgtct ttcgtcaatt gaatttgatg ttgtaattca cgttctctcg
     3181 ttttcatacg attatattgt ttataataat attgagcatt tgctgatggg gattttgtag
     3241 gatttaaagg aatgacaact tcttcattcg tataataatt caatgccgtc acttctttat
     3301 cgccttgctt aattcgatat atattagcag tgatcaattc accatataac tgttcagtat
     3361 ctttattttt agactgctca tattcttcaa tcaacttcgc taatttattt tgatatttgt
     3421 acaactgctg ttgaacaaat cgaactaaat cattcgcacg ttgtttaacg cgttcacgtt
     3481 cgccacgcgc atcataaaaa cgatcaagca aatcatttaa tgaatcgtat gtaactgtat
     3541 catcattaaa ttgatttaac tttataaaat agaaatcctc tttacctgtt tcatgatttt
     3601 tatgaaaaat aggagtaggt ggtaacttgg tttctgccat tacttcgtca aatgcttctg
     3661 gtaatgttga tgaagtaata aattgacgac gactaacgat ttcattcgta attaaagggc
     3721 taaatccttc aaactgattc aataattgtt tagcaatatt acctgcgtta aaatcgatat
     3781 atttcaacac ctctgcacct gtaatatcat acggatttat tttgtgctga gtaggtggtg
     3841 cttcataatt aaatcctggc attactgtac gatagtgatt cgtatttggt gttaagtgtt
     3901 taaatccttc aattattttg cgattttcat ctactaaaat taagttacta tgtttaccca
     3961 taatctcaag gatgacagtg cggtaaatag tatcgccaat ttcatcttta ctctttatat
     4021 cgatttcaat gcgacgatca ttaccaattt gcttaatcga ttcgataata ccaccttcta
     4081 agtgttttct aaaaacacgc gcaaacatgg gtggattaaa tggattatca tattttttag
     4141 tagtcaattg taatcttgaa aagtttggat ggattgacaa taacaattga tggttttgtc
     4201 tattttgacg tacaaccatt agtatcgtgt cattatcagg ttgattgatt ttgtgaacgc
     4261 gtcctgttgt taaaaattgt agagactcaa ccattttctt tgtaaataag ccatcataag
     4321 ccataattta tcttccacct tcttcatatc attcgtttca ttttaacatg attgtaactt
     4381 ttcagtatag ttattgaacc atacattatg ccatacattt tacattatct aaaagtttgc
     4441 aattcaattt atttaattaa aattaatcgt gcaatataac gatattgtta gacttaatag
     4501 aaattatggc atgcaatttc aaatatgcta tacaatataa agaacaatgt gatatcatat
     4561 ttaaataata gaagattagc ttagagaggt cgtaaggcat ggataatgaa aaaggattgt
     4621 taatcgtttt atcaggacca tctggagtag gtaaaggtac tgttagaaaa cgaatatttg
     4681 aagatccaag tacatcatat aagtattcta tttcaatgac aacacgtcaa atgcgtgaag
     4741 gtgaagttga tggcgtagat tactttttta aaactaggga tgcgtttgaa gctttaatca
     4801 aagatgacca atttatagaa tatgctgaat atgtaggcaa ctattatggt acaccagttc
     4861 aatatgttaa agatacaatg gacgaaggtc atgatgtatt tttagaaatt gaagtagaag
     4921 gtgcaaagca agttagaaag aaatttccag atgcgctatt tattttctta gcacctccaa
     4981 gtttagaaca cttgagagag cgattagtag gtagaggaac agaatctgat gagaaaatac
     5041 aaagtcgtat taacgaagcg cgtaaagaag ttgaaatgat gaatttatac gattacgttg
     5101 tagttaatga tgaagtagaa cttgcgaaga atagaattca atgtattgta gaagctgagc
     5161 acttaaaaag agagcgcgta gaagctaagt atagaaaaat gattttggag gctaaaaaat
     5221 aatgttaaat ccacctttaa accaattaac gtcacaaatt aaatcaaagt atttaattgc
     5281 aacaactgca gcgaaaagag cgcgtgaaat tgatgaacaa cctgaaactg aattattaag
     5341 tgaatatcat tcatttaaac cagttggtag agcgttagaa gaaattgctg acggtaaaat
     5401 tcgccctgtt atttcaagtg attattatgg taaagaatag tttcgaccat taaaaatatg
     5461 tgaagctaga cgtacatcaa atacgtcaag gcatatccaa atcaaacgca cctatgtagg
     5521 tgtgtttttt tagtttatcc aatgattgat gttataataa tactaaattt gtatctataa
     5581 aaaagtaatg agcatttgtg cgcatatgat gatgtaaagc gtaaatgtca tagtgatatt
     5641 taagggagat tgaatatgaa gaaaatatta ttagccgtta caggtggcat tgcggcatat
     5701 aaagcaattg atttgacaag taagttaaca caatctgggt atgaagttcg cgttatgtta
     5761 acgaatcacg cacaaaaatt tgtgacacca ttagcatttc aagcaataag tcgaaatgct
     5821 gtttatacag atacttttat agaagaaaat ccttcagaaa tacagcatat tgcattaggt
     5881 gattgggcag atgcaatcat tgttgcacct gcaacggcaa atacaattgc aaaattgagt
     5941 gtaggtattg ctgatgattt ggtgacatca acgttgctag caacagagac accgaaattt
     6001 attgcgcctg ctatgaatgt gcatatgtat gaaaataaac gtacgcagca aaatattaat
     6061 attttaaaag aagatgggta tcattttatc gaaccaggaa gcggatttct agcatgtggt
     6121 tatgttgcta aaggacgtat ggaagaaccg cttcaaatcg tttctgtaat tgatgctcat
     6181 tttcaaaata gtaatcgttt agctaatagt tcatttcaag ataaacgcgc attggttaca
     6241 gcaggaccaa ctattgaagt tatcgatcca gtcagatttg tatccaatcg ttcttctgga
     6301 aaaatgggct atgcaatagc tgaagcattg cgaaatcgag gagctatcgt gacgttagtt
     6361 gctggtccta caacactaga ggatccaaaa gatattgaag ttattcatgt tcaaagtgct
     6421 gaagaaatgt ttgaacaagt gacaagccga tttgacgaac aagatattgt tgtaaaagca
     6481 gcagccgtat ctgactatac accggttgat gtattagaac ataagatgaa aaagcaggat
     6541 ggtgatttgt cagtatcttt taaacgtact aaagacattc ttaaatattt aggtgaacat
     6601 aaaacatcac agtatttaat aggctttgca gcagagactg aagatattga aaattatgca
     6661 caacaaaaat tacgcaagaa aaatgcagat gtgattattt caaataatgt tggggatatg
     6721 tctatcggat ttagttctga tgataatgaa ttgacaatgc attttaaaaa taatgaaaaa
     6781 gtaaatatca agaaaggaaa aaaagtagta ttagctgcac aaattttaga tgaactagaa
     6841 actaggtggc aataatgata gcgaaagtca tagtcgatgt cgcgtcgaag agcgttgact
     6901 ataaatttga ttatataatt cccgaacaac tcgaatctgt catccaacct ggtgtgcgtg
     6961 taattgtacc ttttggacca agaacgattc aaggttatgt aatggaagta acagcagaac
     7021 ctgatgcaca acttgacgtt tcgaagttaa aaaaaatcat agaagtgaaa gatatacaac
     7081 cagaattaac atcagaatta atagctttaa gtgagtggat gggttcaact catgtcatta
     7141 aacgtatttc tatgctagaa gtgatgcttc cgagtgctat taaagcgaag tataaaaaag
     7201 catttaagat gaaagatgac atagagctac cttcagcttt attacaaaaa tttgataagc
     7261 atggttacta ttattataaa gatgcgcaaa aaaataatga tattcaattg cttatgaagt
     7321 tgttaaaaga tgatatcgtt gaagaaaaaa cgattctcac acaaaatata actaaaaaaa
     7381 ccaagcgtgc tgttcgtgtc attgaagggt atcatcctga tgaagtatta gctaagttgg
     7441 agaaagttat taaacaatac gatttgtatg cttacttgtc tgaagaacaa cataaaacaa
     7501 tatttttaac tgatattgag gatatgggct tttcaaaatc cagtttagat ggacttatca
     7561 aaaaaggtta tgttgaaaaa tatgacgcgg ttgttgaaag agacccattt aaagatcgtg
     7621 ttttcgaaca agaatcaaaa cagcaattaa cagaagacca atataaagca tatgaagcga
     7681 ttaaagctaa aattgtaagc caagagcaag aaacattttt acttcatggt gtgacgggat
     7741 caggtaaaac agaagtatat ttacaaacga tagaagatgt tttaagccaa ggaaaacagg
     7801 cgatgatgtt agttcctgaa atcgctctaa caccgcaaat ggttttacgc ttcaaacgtc
     7861 gatttggtga tgacgttgct gtattacatt ctggcttatc taatggggaa cgttatgatg
     7921 aatggcaaaa aattagggat ggtcgtgcga gagtaagtgt tggtgcaagg tcaagtgtgt
     7981 tcgcaccttt caaaaattta gggttaatca tcattgatga agaacatgaa tctacatata
     8041 aacaagaaga ttatccgaga tatcacgcta gagaaattgc ccaatggcga agtgaatatc
     8101 atcactgtcc agtcatttta ggaagtgcaa caccatgtct tgaaagttat gcacgagctg
     8161 aaaaaggcgt ttatcatttg ctatcattac caaacagagt gaaccaacaa gctttacctg
     8221 aaattgatat agtagacatg cgtgaagaat tgagtgaagg taatcggtca atgttttcaa
     8281 aagatttacg tgaagccata caattaagat tagatcgaca ggaacaagtt gttttatttt
     8341 taaatcgacg tggttatgca tcgtttatgt tatgtcggga ttgtggatat gtaccgcaat
     8401 gtccaaactg tgatatttca ttaacgtatc ataaaacgac agacttatta aaatgtcact
     8461 attgtggtta ccaagagacg ccaccgaatc aatgtccaaa ttgtgagagt gaacacattc
     8521 gacaagtagg tactggtact cagaaagttg aagaactatt gcaacaagaa tttgaagatg
     8581 cgcgcataat taggatggat gtagatacaa cctcaaagaa aggtgcacat gaaaagttat
     8641 tgactgaatt cgaaaaaggt aacggtgaca ttttactagg tactcagatg attgcgaaag
     8701 gattagatta tccaaatatt actttagttg gtgtgctgaa tgcagataca atgttaaatt
     8761 tacctgattt tcgggcgagc gaacgtactt atcaactatt aacgcaagtg gctggtagag
     8821 ctggtcgtca tgaaaaggca ggtcaagtca tcattcaaac gtataatcca gatcattatt
     8881 caatattgga tgttcaaaaa aatgattatt taacatttta tcgtcaggaa atggaatatc
     8941 gtaaattagg aaagtatcca ccgtattatt atttgattaa tttcacaatc tcacataaag
     9001 aaatgaagaa ggttatggta gcatcgcagc atgttcataa aattttatta cagcatttaa
     9061 cagaaaaagc gcttgtacta ggtccatctc cggcagcact tgcgagaatc aacaatgaat
     9121 ttagattcca aattttagtg aaatataaaa gtgaacctgg attattacaa gccattcagt
     9181 ttttagatga ctattaccat gaaaaattta taaaagaaaa attagcattg aagattgata
     9241 ttgatccaca gatgatgatg taacattact aattattaga aacaagttca gtatgtacga
     9301 gtatttgaac cagtgtttga atatttactt taagtacaga aaaagggcag aatatacaac
     9361 tgttaactat ttaaattagc agtttatatt ctgtcttttt atatggcttt ataacttacg
     9421 tgattttggt ttgataagga atttattagt attttcattt acgaattctg attttaatgc
     9481 aagcgccata cttactgatg aataaaagta ctaatgatta aacaaactat ttttaaatgg
     9541 caaattgaaa tagtgcgaat tttaaactta taaattaatg gctataaact attttatatc
     9601 tatctatgtt ttaactactt aaattattag acaaaacaat tttcttaaat tatcaattaa
     9661 agtatttaaa aatattgatg gttaatgtaa aatatcaata taaataagtt ttttaaattt
     9721 atgtatgttt atttgattca aacaaaataa cttaagagga gaaagtttat gaaaaagaca
     9781 ctgggatgtt tacttttaat tatgctttta gtcgtagcag gttgttcttt tggtgggaat
     9841 cataaattat catcaaagaa atcagaagaa tcaaaacaag aaactgtaaa aaaagaatcg
     9901 gaagaagaga aagatccaga tttagagaaa tatgaagaaa tagagaagaa aatgaaagga
     9961 attaaagatg cgccatctct tgataagttg gatccattaa tgacagaaaa gtcgtttacg
    10021 aatagtaaag ggattcaagg atggaaagat tacaaagaat taatgggtaa agtggaactt
    10081 gcagattata gatttactaa agattcaaaa ggatcttcaa taaaagatgt tgatgcattc
    10141 tttaaaggta agaaaggtat aaaaaggaaa gtgattgaaa cacacgatga tgtaaaacaa
    10201 gttgattatt ggtatgtaga tccagatgga aagaaaattg gcaattcaaa cacacctgtt
    10261 ttttacgcag aaattatgac aaaatataaa gatggaaagt tagtttatgc atcagtcgaa
    10321 ccaggatctt acgtaataca taaagatgat gcaattaaat atgacgatta ttctaagtta
    10381 aaaaaattaa gtcagctaac taaacttgat catccaaaac cagttccata tagcgtagct
    10441 caaatcaaat ctttcggagt acctttaaca agcgtttcat ttatgacaca tggatcaaag
    10501 gatactaaag atgaagtgtt gccggcattg gcctatttca ctttttcacc aaaaaattat
    10561 gaagacaagt ctaatccaga tccaaaagtt ttaaatttag tacatatgga tttcttaaat
    10621 gcatctagtg attttggtaa cgcacatttt gttgttttaa gtaaatatat taaagagtat
    10681 gaatcaaact atgaaacagc gtcagatgat tctttaaaat agtatttact gtgtgaaaaa
    10741 taaatagtgt actacattaa ataatcgcaa taataatccc gataaacaat cagcattact
    10801 gcttatcaca tagagttcgt aataactata actctatgat tcgcaaataa taaatgattg
    10861 tcatcgggat ttatttttat caatttataa agtgacatta ccttgttcat cagcaggttt
    10921 gaaaacagta atcactgcac taataattgc taaaatgtgt gggatacctg tccaacagaa
    10981 tattaagtgt agaatacctt gcatattctt gccggcataa aatttatgaa taccaaaact
    11041 acctaagaac aatgctaata aaatataaat aactttgttt acttgcattt ctttccctcc
    11101 agttgaattg cttataatga cattagcttc tctttttatt atacccactt ttagttcaaa
    11161 cattctagtt taagcattcc caatcatcta aatttcagtt attcaatcct tacaataaat
    11221 ttaggattac atttcagttg cattgtatta ttttacgtgt gaaatatacg taatgaatca
    11281 catgacaatc ttcaaattga aaaatataca ttctatgatg taaggtcgca tttttaatat
    11341 atttacgtta taatagtttg atgaatttta ttgaggagta agagttatgg cgattaaaaa
    11401 gttagtacca gcatcgcatc ctattttaac gaaaaaagcg caagcagtta aaacatttga
    11461 tgattcgtta aaaagattat tacaagattt agaagataca atgtatgcac aagaagctgc
    11521 tggcttatgt gcacctcaaa ttaatcagtc attgcaagtg gcaattattg atatggaaat
    11581 ggaaggatta ttacaacttg ttaatccgaa aattattagt caatcaaatg aaacaataac
    11641 agacttagaa ggttcaatta cattgccaga tgtttacggc gaagtgacaa gaagtaaaat
    11701 gatagttgtc gaaagttatg acgtcaatgg gaacaaagtt gaactaactg cacatgaaga
    11761 tgtagcaaga atgattttgc atattataga tcaaatgaac ggtatccctt ttacagaacg
    11821 tgcggaccgt attttaacag ataaagaagt ggaggcatat tttataaatg actaaaataa
    11881 tatttatggg tacaccagac ttttcaacaa ctgttttaga aatgcttatt gcagaacatg
    11941 atgtcattgc agtcgtaacg caaccagatc gacctgttgg acgtaaacgt gttatgacac
    12001 caccaccagt taaaaaagtt gcaatgaaat atgatttacc tgtatatcaa cctgaaaaat
    12061 taagtggatc agaagaatta gaacaattgc ttcaattaga tgtagattta attgtaactg
    12121 ctgcttttgg acaattatta cctgaatcat tgttggcatt accaaatctt ggggcaatta
    12181 atgtacatgc atcattgtta ccgaagtata gaggtggtgc accaattcat caggcaatta
    12241 tcgatggtga acaagaaacc ggcataacaa ttatgtatat ggttaaaaaa ttagatgcgg
    12301 gtaatattat ttcgcaacaa gcaattaaaa tagaagaaaa tgataatgtc ggtacgatgc
    12361 atgataaatt aagtgtatta ggggcagatt tattaaaaga aactttacca tctattatag
    12421 agggcacaaa tgaaagcgta cctcaagatg atacgcaagc aacatttgct tccaatattc
    12481 gacgcgaaga tgagcgaatt agctggaata aaccaggaag acaagtgttt aatcaaattc
    12541 gtggattatc accatggcca gttgcttata caactatgga tgacactaac ttgaaaatat
    12601 acgatgctga actcgttgag actaataaga taaacgagcc tggaaccatt atagaaacga
    12661 ctaaaaaagc cattattgtt gctacaaatg ataatgaagc tgttgcaatt aaagatatgc
    12721 aattagctgg gaaaaagaga atgttagctg ccaattattt aagtggtgcg caaaacacac
    12781 tagtagggaa gaaacttata tgatagaaaa cgtgagaagt cttgcttttg acacgattca
    12841 agatatatta aatgaaggtg cgtatagtaa cttgcgtatc aatgaagtgt tgtcagaaaa
    12901 tgaattaaat gcaatggata aggctttatt tacagaaatt gtctacggaa ccgttaaaag
    12961 aaaatatacg ttagattttt atttaaagcc ttttgtgaaa acaaagatta aggcatgggt
    13021 taggcaatta ttatggatga gtatttatca atatgtttat ttagataaag ttccaaatca
    13081 tgccattatt aatgaagcag ttgaaatagc aaaagaacgc ggtggctatc ataatggtaa
    13141 tgtcgtaaat ggtattttac gtacaatgat gcgtagtgac ttacctgatt ttaatgaaat
    13201 tgcagatcct aaaaaaagaa tggcaatcga gtatagtatg ccgaagtgga ttatagatca
    13261 ttgggcaaca cattatggtc tcgaagaaac tgaaacaatt ttacagtcat ttttagaaac
    13321 gacatcaaca actgtgcgtg ccaacctgac gcgagcatca ttagatgata ttattgaaaa
    13381 gttgcaagac gaaggttatg acgttgaaaa agatcatgac ttaccttatt gtctccatat
    13441 aggaggacaa ccaattattc attctcgttc atttaaagat ggattcgttt caattcaaga
    13501 taaaagctca atgtttgttg cacacattat gaatgtagac cgacatgatc acgtattaga
    13561 tgcatgtagt gcacctggcg gtaaagcttg tcacattgct gaagttttaa tgccagaagg
    13621 gcaagttgac gcttcagata tacatgatca caaaatagac ttaattaatt ttaatataaa
    13681 aaaattacga ttaacaaata ttaaagcttt tcaacatgat gcgacaaaac cttatgataa
    13741 aacatacgat aagatacttg ttgatgcacc atgtagcgga ttaggtgtaa tgagacataa
    13801 gccggagatt aagtatactc aaagcaaaca acatattgag tcactagttg aattacagct
    13861 tgaaatattg gaaaatgtaa aaaacaatgt aaaaataggt ggcgaaatca tctattcaac
    13921 atgtacaatt gagcaactag aaaatgaaaa cgtgatttat acgtttttga aaaataataa
    13981 aaacttcgaa tttgaaccgt ttcaacatcc gataactgga gagttggtca aaacgttaca
    14041 aatcatgccg caagacttta attcagatgg attctttatc actaagataa aaagaaagga
    14101 caattaggaa tgataactgc tgaaaagaaa aagaagaata aatttcttcc aaattttgac
    14161 aagcaatcaa tatattcatt gcgatttgac gaaatgcaaa actggctcgt tgaacaaggg
    14221 caacaaaaat ttcgagcgaa acagattttt gaatggttat atcaaaaaag agtagattcg
    14281 attgatgaaa tgacgaactt atcgaaagac ttacgacagc ttttaaaaga taactttact
    14341 gttacaactt taacaactgt agtaaaacaa gaaagtaaag acggtacaat taaattctta
    14401 tttgaattac aagatggcta tacaattgaa actgttttaa tgagacatga ttatggaaat
    14461 tcagtatgtg taacgacaca agtaggttgt cgcatcggat gtacgttttg tgcttctaca
    14521 cttggcggct taaaaagaaa ccttgaagct ggcgaaattg tttcacaagt tttaacagtt
    14581 caaaaagccc ttgatgctac agaagagcgc gtatctcaaa ttgtcataat gggtatcggt
    14641 gaaccatttg aaaattatga tgaaatgatg gactttttaa gaatcgtcaa tgatgataac
    14701 agtttaaata ttggtgcacg tcacattaca gtatcaacat caggtatcat tcctagaata
    14761 tatgactttg cggatgaaga tattcaaatt aattttgctg taagcttaca cgccgcaaaa
    14821 gatgaagtgc gatcacgctt gatgccaatt aaccgtgcat ataatgttga gaagttaatc
    14881 gaagcaattc aatattatca agaaaaaaca aatcgtcgtg ttacttttga atatggtctg
    14941 tttggtggtg tgaatgacca actagaacat gcaagagaat tagcacattt aataaaaggc
    15001 ttaaactgcc atgttaactt aattcctgtc aaccatgttc cagaaagaaa ttatgtgaaa
    15061 acggctaaaa atgatatctt taaatttgaa aaagaattaa agagactagg aattaatgcc
    15121 acaatacgtc gtgaacaagg ttcggatatt gacgcagctt gtggtcaatt aagagcaaag
    15181 gaacgacaag tagaaacgag gtaaagacaa atgctagagg cacaattttt tactgatact
    15241 ggacaacata gagataagaa tgaagatgcg ggtggtattt tttataatca aactaatcaa
    15301 caacttttag ttctgtgtga tggtatgggt ggccataaag caggagaagt tgcaagtaaa
    15361 tttgttacag atgagttgaa atcccgtttt gaagcggaaa atcttataga acaacatcaa
    15421 gctgaaaatt ggttgcgtaa taatataaaa gatataaatt ttcagttata tcactatgca
    15481 caagaaaatg cagaatataa aggtatgggt acaacatgtg tttgtgcact tgtttttgaa
    15541 aaatcagttg tgatagcaaa tgtcggtgat tctagagcct atgttattaa tagtcgacaa
    15601 attgaacaaa ttactagtga tcactcattt gttaatcatc ttgttttaac gggtcaaatt
    15661 acgccggaag aagcatttac acatccacaa cgtaatatta ttacgaaggt gatgggcaca
    15721 gataaacgtg tgagtccaga tttgtttatt aagcgattaa atttttatga ttatttatta
    15781 ttaaattcag atggattaac tgattatgtt aaagacaatg aaattaagcg tttgttagta
    15841 aaagaaggta caatagaaga tcatggtgat caattaatgc aattggcatt agataaccat
    15901 tcgaaagata acgttacttt catactcgcg gctattgaag gtgataaagt atgataggta
    15961 aaataataaa tgaacgatat aaaattgtag ataagcttgg cggcggtggc atgagtaccg
    16021 tttatcttgc tgaagatacg atacttaaca ttaaagttgc aattaaggcg atttttatac
    16081 cacctagaga aaaagaagaa acattaaaac gttttgaacg agaagtacat aactcatcac
    16141 agctatcaca tcaaaatata gtaagtatga tcgatgttga tgaagaagat gactgttact
    16201 acttagtaat ggaatatatc gaaggtccga ctttgtctga gtatattgaa agtcatgggc
    16261 cattaagtgt tgacacagcg attaatttta cgaatcaaat attggatggc attaaacatg
    16321 cgcatgatat gcgtattgta catagagata ttaagccaca aaatatatta attgacagca
    16381 ataaaacgtt gaaaatattt gattttggaa ttgctaaagc tttaagtgag acgtctttaa
    16441 ctcagactaa tcatgtgtta ggtactgtgc agtacttttc gccagaacaa gcaaaaggtg
    16501 aggcaacgga tgaatgtaca gatatttatt ctataggtat tgtgttatat gaaatgcttg
    16561 ttggtgaacc accctttaat ggagaaactg cagttagcat tgcgattaaa catattcagg
    16621 attctgtgcc aaatgtgaca acagatgtac gtaaggatat tccgcaatct ttaagtaatg
    16681 tcattttacg cgctacagaa aaagacaaag cgaatcgtta caaaacaatt caagaaatga
    16741 aagatgattt gagtagtgtt ttacatgaaa atcgagcgaa tgaagatgtc tatgaactcg
    16801 ataaaatgaa aacgatagcg gtacctttga aaaaagaaga tctagcaaag catattagtg
    16861 aacataagtc gaatcaacct aaacgtgaaa cgacgcaagt acctattgta aatgggcctg
    16921 ctcatcatca gcaattccaa aagccagaag gtacggtgta cgaaccaaaa cctaaaaaga
    16981 aatcaacacg aaagattgtg ctcttatcac taatcttttc gttgttaatg attgcacttg
    17041 tttcttttgt ggcaatggca atgtttggta ataaatacga agagacacct gatgtaatcg
    17101 ggaaatctgt aaaagaagca gagcaaatat tcaataaaaa caacctgaaa ttgggtaaaa
    17161 tttctagaag ttatagtgat aaatatcctg aaaatgaaat tattaagaca actcctaata
    17221 ctggtgaacg tgttgaacgt ggtgacagtg ttgatgttgt tatatcaaag ggccctgaaa
    17281 aggttaaaat gccaaatgtc attggtttac ctaaggagga agccttgcag aaattaaaat
    17341 cgttaggtct taaagatgtt acgattgaaa aagtatataa taatcaagcg ccaaaaggat
    17401 acattgcaaa tcaaagtgta accgcaaata ctgaaatcgc tattcatgat tctaatatta
    17461 aactatatga atctttaggc attaagcaag tttatgtaga agactttgaa cataaatcct
    17521 ttagcaaagc taaaaaagcc ttagaagaaa aagggtttaa agttgaaagt aaggaagagt
    17581 atagtgacga tattgatgag ggtgatgtga tttctcaatc tcctaaagga aaatcagtag
    17641 atgaggggtc aacgatttca tttgttgttt ctaaaggtaa aaaaagtgac tcatcagatg
    17701 tcaaaacgac aactgaatcg gtagatgtac catacactgg taaaaatgat aagtcacaaa
    17761 aagttaaagt ttatattaaa gataaagata atgacggttc aactgaaaaa ggtagtttcg
    17821 atattactag tgatcaacgt atagacattc ctttaagaat tgaaaaagga aaaacagcaa
    17881 gttatattgt taaagttgac ggtaaaactg tagctgaaaa agaagtcagc tatgatgatg
    17941 tataaatata attgaagtaa atgtaccgag gtttctattt ggaagtctcg gtatttttat
    18001 gttgagattg cggtagtttt aaaatgcttc atgtcatcat atacaggtga gcattgtcta
    18061 tcaattctgt atcactctca caatatttat atcatttgaa atgatcattt atatattaat
    18121 tttccagtct ttgatataat aaatgcagta aaattaatcg agaggtgcca ttttgaagac
    18181 aggtcgaata gtgaaatcaa ttagtggggt atatcaagta gacgttaatg gcgaacgttt
    18241 caatacaaaa ccacgaggat tatttagaaa gaaaaaattt tcaccggtag ttggtgatat
    18301 agtggaattt gaagttcaaa acattaacga aggttatatt catcaagtgt ttgagcggga
    18361 aaatgagttg aaaagaccac ctgtaagtaa tatagataca ctagtaattg taatgagtgc
    18421 tgtcgagcca aatttttcaa cgcaattatt agatcgattt ttagttattg cacattcgta
    18481 tcagttaaat gcgagaattt tggtgactaa aaaagataaa acaccaattg aaaagcagtt
    18541 cgaaataaat gagttgttga aaatatatga aaatattggc tatgagactg aatttattgg
    18601 aaatgatgat gatcgaaaaa aaattgtaga agcttggcca gctggactta tagtacttag
    18661 tggtcaatca ggtgtcggta agtccacttt cttaaatcat tatcgtccag aacttaatct
    18721 tgagacaaat gatatatcaa aatcattaaa tcgaggaaag catactacaa gacatgtcga
    18781 actattcgaa cgtcaaaacg gttatattgc agacacacct ggattcagtg ctttagattt
    18841 tgatcatata gataaagatg aaataaaaga ttattttctt gaattaaatc gatatggtga
    18901 aacatgtaag tttaggaatt gtaatcatat caaagaacct aattgtaatg ttaagcatca
    18961 attagagata gggaatattg cgcaatttag atacgaccat tatttacaac tatttaatga
    19021 aatttcaaat agaaaggtta gatattaaat gacaaaacta tatccatcat tattatctgt
    19081 tgattttttg gatttacaac atgaattaaa acgacttgaa gaagcaggcg tcgacggagt
    19141 tcattttgat gttatggatg gtcaatttgt gcctaatata tctattggtt taccaatatt
    19201 agatgcagta agaaaaggca caacattacc tatagacgta catttgatga ttgaaaatcc
    19261 agaaaagtat attgcatcat ttgcagaaca tggtgccgat atgatttcaa ttcatgtcga
    19321 atcaacgcct catattcatc gtgctattca aatgattaaa catttagata aaaaagctgg
    19381 tgtagtaatt aatcctggta caccaatatc acaaattgaa cctattttag acattgttga
    19441 ttatgtacta gtgatgacag ttaacccagg gtttggtggt caatcattta ttgatcaatg
    19501 cgtagaaaaa atagcgggtc ttaatgctat taaaatggaa cgtcaattaa actttgatat
    19561 tgaagttgat ggaggcgtaa ataccgatac agcgaaagtt tgtgttgaaa atggtgctac
    19621 aatgctagta acaggttcat ttttctttaa acaagaggat tataaaaaag tcacacaagc
    19681 attgaaaggt tgagtgtata tgcatataaa tttattatgt tctgatcgac acttgccgca
    19741 agatatttgg gccaaaagta atgaaggtaa atggggcggc gttgatagag gtgctttgat
    19801 tttattgaag catcaaatta tccctttttt ctcagtggga gactttgatt cagtcagtaa
    19861 agaagaacgc caacttctaa cagaacagtt acaaatcaaa ccagttcaag ctgaaaaagc
    19921 tgatacggat ttagctttag cggttgataa agctgttgca cttggatttg atagtattac
    19981 aatttatggt gcaacaggcg gacgattaga tcactttttt ggggcaattc agttattatt
    20041 gaaaaaagca tattataaac atgatgttca tatagaagtt atcgatcaac aaaataaaat
    20101 tgaattattg cctaaaggtc aacatacagt tgaaaaagat aagagttatc cgtacatttc
    20161 atttataccg atgactgatg atgtagaact ttctctagca ggttttaaat ataatttagc
    20221 tagacaaatg cttaatatag gttctacttt aactatttca aatgaaattg agtctttgca
    20281 agcgaaagta actgtacatg atgggttgat tttgcaaatt agaagtacag atttaaatta
    20341 agtatattca aatttgtgag gatttttaat aaagtgcatg agttatttaa taaagtgacg
    20401 tttatagata gaatttataa cataataata gatgaatcac gctgtaatta atgaagatta
    20461 tggcgtgatt tattattgaa aggaaaattg taggtcctag tacattgatt tatgtgccaa
    20521 aatcgatatt gaacccataa gtccaatttg acagagaacc attattaaac gtattaacca
    20581 aattatattg atagttcgaa tttcactatg aaccattatg tattgtttgg taacaaaaaa
    20641 agaactaaga ggttgagctc ttagttctta agtaaatgtg tcctccacat aaggtcacat
    20701 tttttatgtg caatatattt attaaactct agttacttta ccagatttta aagcacgtgc
    20761 agaaacccaa acttttttag gtttaccgtc aactaggatt ctaacttttt gaaggttagc
    20821 gttccatcta cgtttagtag agtttaaagc gtgtgaacgt ctgttaccag tcgaagcttt
    20881 acgacctgtt acgaaacatt gtttacccat atgagtacct cctttaataa atataaatac
    20941 acataactac ttatatactt aattaagata gcatagtttc atttgaaaaa caatgaataa
    21001 ttttcacata aaagtcaaaa atactgagtt ttgtgatata attgtagact gtgaagttat
    21061 gtagtatgat attttagaga aataagtgaa tgaaacattt aaaattttta tttaatgata
    21121 ctacatctat taaaagaaaa cgcctataga taatcgtcat gagtgtaaat tcagtttact
    21181 caaaatataa taggtgattt aagcgtaagt cattctgata agttgtacca ttgatatttt
    21241 ataaaaataa tcaatgaaac ttaaagcgtt tatgactaca ctaagtggta aaattttaaa
    21301 agtgattagg gaaaacgaat aataaacttt gaaatttagg agggacaaag atatgacatt
    21361 agagatttca aatgattacg gtaaaattga tatttcaaac gaagtgattg cttcggttgt
    21421 aggtggaaag gccgttgaat gttatggtat tgtaggtatg gcatctagac aacaagttag
    21481 agatggtatt gcggaaatac taggacatga aaattatgct aaaggcatca aagtaactga
    21541 aaataatggc gtagtggata tagatatgta cattattgta agttacggtg tgaaaatatc
    21601 tgaagttgcc aataatgtac aatcaacagt gaaatatact ttggaaaaat cacttaatgt
    21661 atcagtaaat tcaatcaata tatatgtaca aggtgtacgt gtgaataata caggcaagaa
    21721 agcttaggag gacaacttga aatgattagc aaaattaatg gtaaattatt tgccgatatg
    21781 attatacaag gggcacaaaa tttatctaac aatgcagatt tggtagattc tttgaatgtg
    21841 tatccagtgc cagatggtga tacaggaaca aatatgaatc ttactatgac ttcaggtcgc
    21901 gaagaagtag agaataattt gtcgaaaaat atcggcgaat taggtaaaac attctcgaaa
    21961 ggtttactaa tgggtgcaag aggtaactct ggtgtcatct tgtcacaatt attcagagga
    22021 ttttgtaaaa atattgaaag tgaatctgaa attaattcaa aattgttagc tgaaagtttt
    22081 caagctggtg ttgaaacggc atataaagct gttatgaaac cagttgaagg tacaatactt
    22141 acagttgcaa aagatgctgc gcaagctgca atagaaaaag caaataatac tgaagattgt
    22201 atagaattaa tggagtacat tattgtaaaa gccaatgaat cacttgaaaa cacaccaaac
    22261 ttattagctg tacttaaaga agttggtgtt gttgatagtg gcggtaaagg tttgttatgc
    22321 gtttacgaag gattcttaaa agcgcttaaa ggtgaaaaag ttgaagccaa agttgcaaag
    22381 atagataaag atgaatttgt acatgatgaa catgatttcc atggtgtaat taatactgaa
    22441 gatattattt atggctattg tactgaaatg atggttcgtt ttggaaagaa taaaaaagcc
    22501 tttgatgaac aagaattcag gcaagatatg agtcaatttg gtgattcttt attagtcatt
    22561 aatgatgaag aaattgtgaa agttcacgtg cataccgaat acccaggtaa agtgtttaat
    22621 tatggtcaac aatatggtga attaattaaa cttaaggttg aaaatatgag agaacagcac
    22681 cgtgaagtga ttcgaaaaga acagcacaca gctaaaccga aaatggaaac ggttgaaaca
    22741 gcaattatta ctatttctat gggtgaaggt atttcagaga tatttaaatc aatgggtgcc
    22801 acacatatca ttagtggtgg acaaacgatg aatccttcta cagaagatat cgttaaagtc
    22861 attgaacaat caaaatgtaa acgtgcaatt attttaccga ataataaaaa tatcttaatg
    22921 gcaagtgaac aagcagcgag tattgttgat gcagaagctg ttgttattcc aacgaaatct
    22981 attcctcaag gtataagcgc actattccaa tatgatgtgg acgcaacact tgaagaaaat
    23041 aaagcgcaaa tggctgattc agtaaataac gttaaatctg gttcattaac gtacgctgtt
    23101 cgtgatacga aaattgatgg cgttgagatt aaaaaagacg cgtttatggg cttgattgaa
    23161 gataagattg taagcagcca aagtgatcaa ttaacaacgg ttactgagtt gttaaatgag
    23221 atgttagcag aagatagtga aatattgact gtgattattg gtcaagatgc agagcaagca
    23281 gttacagata acatgataaa ctggatcgaa gagcaatatc cagatgtaga agtggaagtt
    23341 catgaaggtg gacaaccaat ttatcaatat ttcttttcag tagaataaaa atttaaaata
    23401 aaaaactacc aatgataaat catcagttgg tagtttttta ttttgctatt ttagtgatat
    23461 tgcgggttaa aagtatcgtt ctcgagttgc taacaatgtc atgttcaact tagtcatgat
    23521 aaaataaata acatactaaa tgatacgtaa aatcaaataa aacataggtg atttattttg
    23581 gctaaagtaa acttaataga aagtccatat tctcttttac aattaaaagg tataggtcct
    23641 aagaaaatag aagtattgca acaactaaat attcatacag tggaagatct tgttctttat
    23701 ttgccaacta gatatgaaga taatacagtg attgatttga atcaagcaga agatcaatct
    23761 aacgttacga tagaaggaca agtatataca gctccagtag ttgcattttt tggaagaaat
    23821 aaatcaaaat taaccgttca tttaatggta aataatattg ctgtcaaatg tatttttttc
    23881 aatcaaccgt atttaaaaaa gaaaatcgaa ttaaatcaaa ctataactgt taaaggtaag
    23941 tggaataggg ttaaacagga aattactggt aatagggttt tctttaattc acaagggaca
    24001 caaactcaag aaaacgcaga tgttcaatta gaaccagtct atcgtattaa ggaaggtatt
    24061 aaacaaaagc aaatacgaga ccaaattaga caagcgttaa atgatgtgac aattcatgaa
    24121 tggttaactg atgaactaag agaaaaatat aaattagaga ccttggactt tactttgaac
    24181 acattacatc atcctaaaag taaagaggat ttattacgtg ctcgtagaac ctatgcattt
    24241 actgaactgt ttttattcga attacgtatg caatggctaa atagattaga aaagtcatct
    24301 gacgaagcaa ttgaaattga ttatgacata gaccaagtta aatcatttat tgatcgttta
    24361 ccttttgaac taactgaagc acagaaatcc agtgttaatg aaatttttag agatttaaaa
    24421 gcaccaatac gtatgcatcg attacttcaa ggtgatgtag gttcaggaaa aacagtagtt
    24481 gctgcaattt gtatgtatgc gttaaaaact gctggttatc aatcagcatt gatggtacca
    24541 actgaaattt tagcagagca acatgctgaa agtttaatgg ctttatttgg agattctatg
    24601 aacgttgcat tgttaactgg gtcagtaaaa ggtaagaaac gaaagatact tttagaacaa
    24661 cttgaaaatg gtacgattga ttgtttaatt ggaacccatg ctttgattca agatgatgtg
    24721 attttccata atgttggttt agtaattaca gatgaacaac atcgatttgg tgtgaatcaa
    24781 cgccagcttt taagagaaaa aggtgcaatg acgaatgtgt tatttatgac agcaacgccg
    24841 ataccaagaa cactagcaat atcagttttt ggtgagatgg atgtgtcttc aattaaacaa
    24901 ttaccaaaag gtcgtaaacc tatcattact acttgggcaa agcatgagca atacgataaa
    24961 gttttgatgc aaatgacctc agagttgaaa aaaggtcgtc aagcatatgt catttgcccg
    25021 ctaatagaaa gttctgagca tctcgaagat gttcaaaatg ttgtcgcatt gtacgagtct
    25081 ttacaacagt attatggtgt ttcccgtgta gggttattgc atggtaagtt atctgccgat
    25141 gaaaaagatg aggtcatgca aaagtttagt aatcatgaga taaatgtttt agtttctact
    25201 actgttgttg aagtaggtgt taatgtaccg aatgcaactt ttatgatgat ttatgatgcg
    25261 gatcgctttg gattatcaac tttacatcag ttacgcggtc gtgtaggtag aagtgaccag
    25321 caaagttact gtgttttaat tgcatcccct aaaacagaaa caggaattga aagaatgaca
    25381 attatgacac aaacaacgga tggatttgaa ttgagtgaac gagacttaga aatgcgtggt
    25441 cctggagatt tctttggtgt taaacaaagt ggattgccag atttcttagt tgccaattta
    25501 gttgaagatt atcgtatgtt agaagttgct cgtgatgaag cagctgaact tattcaatct
    25561 ggcgtattct ttgaaaatac gtatcaacat ttacgtcatt ttgttgaaga aaatttatta
    25621 catcgtagtt ttgactaatt gccatgctga tttgtcaatt tgagtgcaac acttcgttaa
    25681 ttgagtgata tgacacttga actatttaaa tgtaaagtgg tattttaaca atttataaat
    25741 tttcgactaa ataatagcta aatattacag ttatttgttg agtcggttaa atagaaagtg
    25801 ttatgatatg tgaggaatgt ttaagactag gtactaaaaa atgaggggtg agacgttgaa
    25861 actaaagaaa gataaacgta gagaagcaat cagacaacaa attgatagca atcccttcat
    25921 cacagaccat gaactaagcg acttatttca agtgagtata caaacaattc gtttagatcg
    25981 cacttattta aacataccag aattaaggaa gcgtattaaa ttagttgctg aaaagaatta
    26041 tgaccaaata agttctattg aagaacaaga atttattggt gatttgattc aagtcaatcc
    26101 aaatgttaaa gcgcaatcaa ttttagatat tacatcggat tctgtttttc ataaaactgg
    26161 aattgcgcgt ggtcatgtgc tgtttgctca ggcaaattcg ttatgtgttg cgctaattaa
    26221 gcaaccaaca gttttaactc atgagagtag cattcaattt attgaaaaag taaaattaaa
    26281 tgatacggta agagcagaag cacgagttgt aaatcaaact gcaaaacatt attacgtcga
    26341 agtaaagtca tatgttaaac atacattagt tttcaaagga aattttaaaa tgttttatga
    26401 taagcgagga taaaattatg gttaaattag caattgatat gatgggtggc gacaatgcgc
    26461 ctgatatcgt attagaagcc gtacaaaagg ctgttgaaga ctttaaagat ctagaaatta
    26521 tacttttcgg tgacgaaaaa aagtataatc tgaaccatga acgaatcgaa tttagacatt
    26581 gttctgaaaa gattgaaatg gaagatgagc ctgttagagc gattaaacgt aaaaaagata
    26641 gctcaatggt aaaaatggct gaagctgtga aatctggtga agcagatgga tgtgtgtcag
    26701 caggtaatac tggtgcttta atgtcagctg gtttattcat tgttggacgt attaaaggtg
    26761 tagctagacc ggctttagta gtaacattgc caacgattga tggaaaaggt tttgtctttt
    26821 tagacgttgg tgcaaatgct gatgctaaac ctgaacactt attacagtat gcgcaactag
    26881 gggatattta tgctcaaaaa attagaggta ttgataatcc gaaaatctca ttattaaata
    26941 taggaaccga gccagctaaa ggtaatagtt taacgaaaaa atcatatgag ttattaaatc
    27001 atgatcattc attgaatttt gttgggaata ttgaagcgaa gacattaatg gatggcgata
    27061 cagatgttgt agttaccgat ggctatactg ggaacatggt ccttaaaaat ttagaaggta
    27121 ctgcaaaatc aatcggtaaa atgttaaaag atacgattat gagtagtact aaaaataaat
    27181 tagcaggtgc aatattgaag aaagatttag ctgaattcgc taaaaagatg gattactcag
    27241 aatacggtgg ttccgtatta ttaggattgg aaggtactgt agttaaagca cacggtagtt
    27301 caaatgctaa agctttttat tctgcaatta gacaagcgaa aatcgcagga gaacaaaata
    27361 ttgtacaaac aatgaaagag actgtaggtg aatcaaatga gtaaaacagc aattattttt
    27421 ccgggacaag gtgcccaaaa agttggtatg gcgcaagatt tgtttaacaa caatgatcaa
    27481 gcaactgaaa ttttaacttc agcagcgaac acattagact ttgatatttt agagacaatg
    27541 tttactgatg aagaaggtaa attgggtgaa actgaaaaca cacaaccagc tttattgacg
    27601 catagttcgg cattattagc agcgctaaaa aatttgaatc ctgattttac tatggggcat
    27661 agtttaggtg aatattcaag tttagttgca gctgacgtat tatcatttga agatgcagtt
    27721 aaaattgtta gaaaacgtgg tcaattaatg gcgcaagcat ttcctactgg tgtaggaagc
    27781 atggctgcag tattgggatt agattttgat aaagtcgatg aaatttgtaa gtcattatca
    27841 tctgatgaca aaataattga accagcaaac attaattgcc caggtcaaat tgttgtttca
    27901 ggtcacaaag ctttaattga tgagctagta gaaaaaggta aatcattagg tgcaaaacgt
    27961 gtcatgcctt tagcagtatc tggaccattc cattcatcgc taatgaaagt gattgaagaa
    28021 gatttttcaa gttacattaa tcaatttgaa tggcgtgatg ctaagtttcc tgtagttcaa
    28081 aatgtaaatg cgcaaggtga aactgacaaa gaagtaatta aatctaatat ggtcaagcaa
    28141 ttatattcac cagtacaatt cattaactca acagaatggc taatagacca aggtgttgat
    28201 cattttattg aaattggtcc tggaaaagtt ttatctggct taattaaaaa aataaataga
    28261 gatgttaagt taacatcaat tcaaacttta gaagatgtga aaggatggaa tgaaaatgac
    28321 taagagtgct ttagtaacag gtgcatcaag aggaattgga cgtagtattg cgttacaatt
    28381 agcagaagaa ggatataatg tagcagtaaa ctatgcaggc agcaaagaga aagctgaagc
    28441 agtagtcgaa gaaatcaaag ctaaaggtgt tgacagtttt gcgattcaag caaatgttgc
    28501 cgatgctgat gaagttaaag caatgattaa agaagtagtt agccaatttg gttctttaga
    28561 tgttttagta aataatgcag gtattactcg cgataattta ttaatgcgta tgaaagaaca
    28621 agagtgggat gatgttattg acacaaactt aaaaggtgta tttaactgta tccaaaaagc
    28681 aacaccacaa atgttaagac aacgtagtgg tgctatcatc aatttatcaa gtgttgttgg
    28741 agcagtaggt aatccgggac aagcaaacta tgttgcaaca aaagcaggtg ttattggttt
    28801 aactaaatct gcggcgcgtg aattagcatc tcgtggtatc actgtaaatg cagttgcacc
    28861 tggttttatt gtttctgata tgacagatgc tttaagtgat gagcttaaag aacaaatgtt
    28921 gactcaaatt ccgttagcac gttttggtca agacacagat attgctaata cagtagcgtt
    28981 cttagcatca gacaaagcaa aatatattac aggtcaaaca atccatgtaa atggtggaat
    29041 gtacatgtaa tatatttgag ctaaagctca ttgacgcagt ggttgactgg tcatccaatg
    29101 gagaattgtc tgacctagtc aactttgcgg gggaaattct aagcaaccta gataaggttc
    29161 cagaatttct ccctaagaaa cactaatcaa taaattgata agtgtttcta aaatttctac
    29221 ttgtttttta gaatttaaaa tgggaaaata tagtagtcta tgtataggca tttttaaagg
    29281 aggtgaatcg acgtggaaaa tttcgataaa gtaaaagata tcatcgttga ccgtttaggt
    29341 gtagacgctg ataaagtaac tgaagatgca tctttcaaag atgatttagg cgctgactca
    29401 cttgatatcg ctgaattagt aatggaatta gaagacgagt ttggtactga aattcctgat
    29461 gaagaagctg aaaaaatcaa cactgttggt gatgctgtta aatttattaa cagtcttgaa
    29521 aaataataaa tcttacatct gggtcgtcag tattgtcgac tcagtttttt tctttaatta
    29581 tcaatagttt taacgtaaaa ttaaagatga ttcaagagca acacataaag gagataaaat
    29641 aatgtctaaa caaaagaaaa gtgagatagt taatcgtttt agaaagcgct ttgatactaa
    29701 aatgacagag ttaggcttta cttatcaaaa tattgattta taccaacaag cattttcgca
    29761 ttcgagtttt attaatgatt ttaatatgaa tcgtttagac cataatgagc gtttagagtt
    29821 tttgggtgat gcggtattag aattgacggt ttcacgatat ttatttgata aacatcccaa
    29881 cttgccagaa gggaatttaa caaaaatgcg tgccactatt gtatgtgagc cctcacttgt
    29941 aatatttgcg aataaaattg gattgaacga aatgatttta cttggtaaag gtgaagagaa
    30001 aacaggggga cgtacaagac catcattaat atcagatgca ttcgaagcat ttattggggc
    30061 attgtatttg gatcaaggac tagatatagt ttggaaattt gctgagaaag tcattttccc
    30121 acatgtagaa caaaatgagt tattaggcgt ggtagatttt aaaacacaat tccaagaata
    30181 tgtgcaccag caaaataaag gtgatgtaac ctataattta ataaaagaag agggaccggc
    30241 acatcatcgt ctattcactt cagaagttat tctgcaaggg gaagcaatag ctgaaggtaa
    30301 agggaaaacg aaaaaagaat cagaacaacg tgctgctgaa agtgcctata agcaattaaa
    30361 acaaattaaa tagaaattta tatgatacga catataggtg ttcgatttag gatttaagga
    30421 agaattttta atattttatg gaacaacttc gaaatttaat atacaaatac taaaatatcg
    30481 tatacatgtg ttctttaaaa ttgtgataag gagtttagga tggtttattt aaaatcaata
    30541 gatgccattg gatttaagtc ttttgcagat caaaccaatg ttcaattcga taaaggtgta
    30601 actgcaattg ttggtccaaa tggaagcggt aaaagtaata ttacagatgc tattaaatgg
    30661 gtgttgggtg aacaatcggc taaatcatta cgtggctcaa aaatggaaga tattatcttc
    30721 tcaggtgcag aacatcgcaa agctcaaaat tatgctgaag tacagttaag attagataat
    30781 cattctaaaa agctcagtgt tgatgaaaac gaagttattg taacaagaag attgtatcga
    30841 agtggtgaaa gtgagtacta cataaataat gaccgtgcaa gattaaaaga tattgccgat
    30901 ttatttttag attctggatt gggaaaagaa gcgtatagca ttatctcgca aggtagagtt
    30961 gatgaaatac taaatgctaa accaattgat agacgtcaaa ttattgaaga atcggctggt
    31021 gtacttaaat ataaaaaacg taaggctgaa tcattaaata aacttgacca aacagaagat
    31081 aatttaacga gagtagaaga cattttatat gatttggaag gtcgcgtaga acctctaaaa
    31141 gaggaggcag ctatagctaa agaatataag acactttcac atcaaatgaa acatagtgac
    31201 attgtagtta cagtgcacga tattgatcaa tatacaaatg acaatagaca attagatcaa
    31261 cgtttaaatg atttacaagg ccaacaagca aataaagaag ctgacaagca acgtttaagc
    31321 caacaaattc aacaatataa aggtaaacgt catcaacttg ataatgatgt tgaatcgctt
    31381 aattatcaat tagtaaaagc tacggaagcc tttgaaaaat atacgggaca attaaatgtt
    31441 ttggaagaac gtaagaaaaa tcaatctgaa acaaatgcac gatatgaaga agaacaagaa
    31501 aatttaatgg agcttttaga aaatatatca aatgagattt ctgaagctca agatacttat
    31561 aagtctctga aaagtaaaca aaaagaactc aatgctgtca ttcgtgaact tgaagaacaa
    31621 ctatatgttt cagacgaagc acatgatgaa aaattggaag aaattaaaaa cgaatactat
    31681 acattaatgt cagagcaatc agatgttaac aatgatattc gttttttaaa gcatactata
    31741 gaagagaatg aggctaaaaa atcaagacta gattctcgat tagttgaagt ttttgagcaa
    31801 ttgaaagata ttcagggtca aataaaaacg acaaaaaaag aatatcaaca gaccaacaaa
    31861 gaactttctg ctgtagataa agaaattaaa aatatagaaa aagacctcac tgatacaaaa
    31921 aaagcacaaa atgaatacga agagaaattg tatcaagcat atcgatatac cgaaaaaatg
    31981 aaaacacgta ttgatagttt ggcaacgcaa gaggaagaat atacttattt tttcaatggc
    32041 gtcaaacata ttttgaaagc taaaaataaa gaattaaagg gtatttatgg tgcagttgcg
    32101 gaaattattg atgtgccatc taaattaact caggcaattg aaacagcatt aggtgcttca
    32161 ttacaacatg tcattgtaga ttcagaaaaa gatggacgcc aggctattca atttttaaaa
    32221 gaacgtaatt taggtcgtgc gacgttttta ccattaaatg ttatacagag tagagtggta
    32281 gcgactgata ttaaatctat tgctaaagag gcaaacggat ttattagtat cgcttcggaa
    32341 gcagttaaag tagcaccaga atatcaaaat attatcggga atttattagg taatacgatt
    32401 atcgttgatc atttaaagca tgcaaatgaa ttggcacgtg cgattaaata tcgaactcgt
    32461 attgttactt tggaaggtga tattgtaaat cctggtggtt ctatgactgg tggtggcgct
    32521 cgtaagtcaa aaagtattct gtctcaaaaa gacgagttga caacaatgag acaccaatta
    32581 gaagattact tgcgtcaaac agaatcattt gaacaacaat ttaaagagtt gaagataaaa
    32641 agtgatcaat taagtgaact gtattttgaa aaaagtcaaa agcataatac acttaaagag
    32701 caagtgcatc attttgaaat ggagctcgat agattaacta cacaagaaac acaaataaaa
    32761 aatgatcatg aagaattcga atttgaaaaa aatgatggtt atacgagtga caaaagtcga
    32821 caaactttga gtgaaaaaga aacttatcta gaaagtatta aagcatcttt aaaacgacta
    32881 gaagatgaaa ttgaacgcta cacaaaactt tctaaagaag gtaaggaaag cgttactaaa
    32941 acacaacaaa ccttacatca gaaacaatct gatcttgctg tggttaaaga gcgtattaaa
    33001 acacaacaac agacaataga tcgattaaat aatcaaaatc aacaaactaa acatcaatta
    33061 aaagatgtta aagaaaaaat tgcattcttt aattcggatg aagtgatggg cgaacaagct
    33121 tttcaaaata ttaaagatca aattaatggt caacaagaaa cgagaacacg cttatcagat
    33181 gaattagata aattgaaaca acaacgtatt gagttgaatg aacaaatcga tgcgcaagaa
    33241 gctaaactac aagtttgtca ccaagatatt ttagctatcg aaaatcacta ccaagatatt
    33301 aaagctgaac aatcaaagct agatgtatta attcatcatg cgatagatca tttaaatgat
    33361 gaatatcaat tgactgttga acgtgcgaaa tctgaatata cgagtgatga atcgattgac
    33421 gcattacgta aaaaagttaa gttaatgaag atgtcgattg atgaactagg tcctgtaaac
    33481 ttaaatgcaa ttgaacaatt tgaagagtta aatgaacgtt atacattttt aagtgaacaa
    33541 cgtacagatc ttcgtaaagc taaagaaaca ttagagcaaa ttataagtga aatggatcaa
    33601 gaggttactg aaagatttaa agaaactttc catgctattc aaggacattt tacagctgtg
    33661 ttcaaacaat tgtttggtgg aggcgatgca gaattgcaat taactgaagc cgattattta
    33721 acagctggta ttgatattgt ggtacaacca ccgggtaaaa agttgcaaca tttatcgtta
    33781 ctgagtggtg gtgagcgtgc attaactgct attgctttac tatttgcaat tttaaaagta
    33841 agatctgcac cttttgttat attagatgag gttgaagctg cactagatga agcaaatgtt
    33901 attagatacg caaaatattt aaatgagtta tcagacgaaa cacaattcat tgttattaca
    33961 caccgtaaag gaacaatgga atttgcagat aggttatacg gtgtaacaat gcaagaatca
    34021 ggtgttacta aacttgtgag tgtgaattta aatacaatag atgatgtgtt gaaggaggag
    34081 caataatgag cttttttaaa cgcttaaaag ataagtttgc aacaaataaa gaaaatgaag
    34141 aagttaaatc cttaacagaa gaacaaggtc aagacaaatt agaagataca cattctgaag
    34201 gttcaacgca ggacgcaaat gatttagcag aaaatgctga agtgaaaaag aagccacgca
    34261 agttgagtga agcggatttt gatgacgatg gcttaatatc aattgaagat tttgaagaaa
    34321 ttgaagctca aaaaatgggt gctaaattta aagcaggact cgaaaaatct cgtcaaaatt
    34381 tccaagaaca attaaataat ttgatagcga gatatcgtaa agtagatgaa gacttttttg
    34441 aagctttaga agaaatgtta atcactgcag acgtcggttt taatacagtg atgacgttaa
    34501 ctgaagaatt acgtatggaa gcacaacgac gtaatattca agatactgaa gatttgcgtg
    34561 aagtcattgt tgaaaaaatc gtagagattt accatcaaga agatgataat tcagaagcta
    34621 tgaacttaga agatggtcgt ttaaatgtca ttttaatggt tggtgtgaat ggtgttggta
    34681 aaacaacaac aattggaaaa ttagcttacc gatataaaat ggaaggtaaa aaagtaatgt
    34741 tagctgcggg cgatactttt agagcgggtg ctattgatca attgaaagtt tggggcgaac
    34801 gtgttggtgt agacgtaatt agccaaagtg aaggttctga tccagctgct gttatgtatg
    34861 atgcgattaa tgccgctaaa aacaaaggtg ttgatatttt aatctgtgat accgctggac
    34921 gtttacaaaa taaaacaaat ctaatgcaag aattagaaaa agttaagcgt gtaattaatc
    34981 gagcagtgcc agatgcgcct catgaagcat tactatgttt agatgctaca actggtcaga
    35041 atgcgttgtc acaagctaga aactttaaag aagtaacaaa tgttacaggt attgtattaa
    35101 cgaaattaga tggtacagcc aaaggtggta tcgtattagc cattcgtaat gaattgcaca
    35161 tcccagttaa atatgtaggt ttaggtgagc aattagatga cttacaacca tttaaccctg
    35221 aaagttatgt ctacggctta ttcgctgata tgattgaaca aaatgaagaa ataacaacag
    35281 ttgaaaatga tcaaattgta acagaagaaa aggacgataa tcatgggtca aaatgattta
    35341 gttaaaacgt tacgaatgaa ttatttgttt gatttttatc aatccttatt gacgaataaa
    35401 caacgtaatt atttggaatt attttatctt gaagattatt ctttaagtga aatcgcagat
    35461 acttttaatg tgagtagaca agcagtttat gataatataa gaagaactgg cgatttagtt
    35521 gaagattatg aaaagaaatt ggaattatac cagaaatttg agcaacgccg agaaatatat
    35581 gatgaaatga aacaacattt aagtaatcca gaacaaatac aacgttatat tcaacaatta
    35641 gaagacttag aatagatata aattgaggga ggacatcgat atggcatttg aagggttatc
    35701 agaacgcttg caagcgacga tgcaaaaaat gcgtggtaag ggtaaactta ctgaagctga
    35761 tataaagata atgatgcgtg aagtaagatt agcgttactt gaggctgacg taaactttaa
    35821 agtggtaaaa gaatttatta aaacagtatc agaacgcgca ttaggttccg atgtaatgca
    35881 atcattaaca ccagggcaac aagttattaa aatagttcaa gatgaattaa cgcagttgat
    35941 gggtggagaa aatacgtcga ttaatatgtc aaataaacca cctactgttg ttatgatggt
    36001 tggtttacaa ggtgctggta aaacaacaac tgcaggtaaa ttagcattat tgatgcgtaa
    36061 aaaatacaac aaaaaaccta tgttagttgc agcagatatt tatcgtccag cagcgataaa
    36121 tcaattacaa acagtaggga aacaaattga tattcctgta tacagtgaag gagatcaagt
    36181 aaagccacaa caaattgtaa ctaatgcatt aaaacatgct aaagaagaac atttagactt
    36241 tgtaatcatt gatacagcag gtcgattaca catcgatgaa gcattgatga acgaattaaa
    36301 agaagtaaaa gacattgcta aaccaaacga aattatgtta gttgtcgatt caatgacggg
    36361 tcaagatgct gtcaatgttg cagaatcttt tgacgatcaa cttgatgtca caggtgttac
    36421 cttaactaaa ttagatggtg atacacgtgg tggtgcagct ttatctattc gttcggtgac
    36481 acaaaaacca attaaatttg ttggtatgag tgaaaagtta gatggtttag agctattcca
    36541 tcctgaacgt atggcatcac gtattttagg tatgggtgat gtgttaagtt taattgaaaa
    36601 agcgcaacaa gatgtggatc aagaaaaagc aaaagattta gagaaaaaga tgcgtgagtc
    36661 atcgtttact ttagatgatt ttttagaaca acttgatcag gtgaaaaatc taggaccact
    36721 ggatgatatt atgaaaatga ttccaggtat gaataaaatg aaagggctag ataagcttaa
    36781 tatgagtgaa aagcaaattg atcatattaa agcgattatc cagtcaatga cgccggctga
    36841 aagaaacaat ccagacacat tgaatgtatc acgtaaaaag cgtattgcta aagggtctgg
    36901 tcgttcatta caagaagtca atcgtttgat gaaacaattt aacgatatga agaaaatgat
    36961 gaaacagttc actggtggcg gtaaaggtaa aaaaggtaaa cgcaatcaaa tgcaaaatat
    37021 gttaaaaggt atgaatttac cgttttaatg tttaatataa cacgctgatt tttgttgcaa
    37081 tgagatgtgc atcccgaaag ttatactaaa gaatataact ttatggggtg catattttta
    37141 gatacacaat aataattaaa aactgaaatt gctgatttgc tagaacatga ggttattgtt
    37201 gctaatatta ataagttata ttgggggaac ttgtgcggta gaagtggtac cattcatgtc
    37261 cttacgagat aaattaactt aacacgtagg tggtgaaatt tactatgaat ttttataacc
    37321 tattaacagt ctaatttagt aatcacatag ttattattgt aagaaaaagg ttcaataatt
    37381 ttcctggtaa agaaaaaact ctttacaaac attcatacac ctgttaatat tatttcttgt
    37441 agaaaataaa aattaaaaca tgacttaaag gagattttat aaatggcagt taaaattcgt
    37501 ttaacacgtt taggttcaaa aagaaatcca ttctatcgta tcgtagtagc agatgctcgt
    37561 tctccacgtg acggacgtat catcgaacaa atcggtactt ataacccaac gagcgctaat
    37621 gctccagaaa ttaaagttga cgaagcgtta gctttaaaat ggttaaatga tggtgcgaaa
    37681 ccaactgata cagttcacaa tatcttatca aaagaaggta ttatgaaaaa atttgacgaa
    37741 caaaagaaag ctaagtaatt tagcgtaaaa ttgttctaac aataagaata actcgtttac
    37801 actgacagtt attactcaat gatacgttgg gaatatcaca tgttagtaat atagaacgta
    37861 ttgggtacca taatggtgcc ctttttcttt gaattatttt caattaaaat agaagtgttc
    37921 aaagcataga gttggaggta atagaatgag agttgaagtt ggtcaaattg ttaacacaca
    37981 tggtattaaa ggtgaaatta aagtaaaatc caattcagac tttacagacg ttcgttttca
    38041 acccggtcaa gtgctgacag ttgtgcataa caataacgac cttgaatata ctgttaagtc
    38101 acatagagtg cataaagggc ttcatatgct tacatttgaa ggtattaata atattaatga
    38161 tattgagcat ttaaaaggga gttctattta tcaagagcgt gatcatgaag atatcgtact
    38221 tgaggaaaat gaattttatt attcagatat tataggatgt acagtttttg atgatcaaga
    38281 aacaccaata ggtcgtgtaa ttaatatatt tgaaacaggt gcgaatgatg tgtgggtgat
    38341 taaaggatct aaagaatatt tgattcctta tattgctgat gttgtaaaag aagtggatgt
    38401 tgaaaataaa aaaattatca tcacgccaat ggaaggattg ttggattaat gaaaattgat
    38461 tatttaactt tatttcctga aatgtttgat ggtgttttaa atcattcaat tatgaaacgt
    38521 gcccaagaaa acaataaatt acaaatcaat acggttaatt ttagagatta tgcaattaac
    38581 aagcacaacc aagtagatga ttatccgtat ggtggcggac aaggtatggt gttaaagcct
    38641 gaacctgttt ttaatgcgat ggaagactta gatgtcacag aacaaacacg cgttatttta
    38701 atgtgtccac aaggcgagcc attttcacat cagaaagctg ttgaattaag caaggccgac
    38761 cacatcgttt tcatatgcgg acattatgaa ggttacgatg aacgtatccg aacacatctt
    38821 gtcacagatg aaatatcaat gggtgactat gttttaactg gtggagaatt gccagcgatg
    38881 accatgactg atgctattgt tagactgatt ccaggtgttt taggtaatga acagtcacat
    38941 caagacgatt cattttcaga tgggttatta gagtttccgc aatatacacg tccgcgtgaa
    39001 tttaagggtc taacagttcc agatgtttta ttgtctggaa atcatgccaa tattgatgca
    39061 tggagacatg agcaaaagtt gatccgcaca tataataaaa gacctgactt aattgaaaaa
    39121 tatccattaa ctaatgcaga taagcaaata ttagaaagat ataaaatagg attgaaaaaa
    39181 ggttagtatt gtgctaacat aatctgagtg tggaaacact aaaatcacta tgatccgctg
    39241 ctatatattt gtcgaggcaa gaacataggt tgaagaggag aatttataat gacaaatcac
    39301 aaattaatcg aagcagtaac taaatcacaa ttacgtacag acttaccaag tttccgtcct
    39361 ggtgatactt tacgtgtaca cgtacgtatc attgagggta ctcgtgagcg tatccaagta
    39421 ttcgaaggcg ttgtaattaa acgtcgtggc ggtggcgttt ctgaaacgtt tacagttcgt
    39481 aaaatttcat caggtgttgg cgtggaacgt acattcccat tacacacacc aaaaattgaa
    39541 aaaatcgaag ttaaacgtcg tggtaaagta cgtcgtgcta aattatatta cttacgtagt
    39601 ttacgtggta aagctgctag aatccaagaa attcgttaat cagcatttaa acaaagctat
    39661 gtatgagtca aattcgactc aaacaataaa gccatctagg tcacttttat ggagtgatat
    39721 agatggcttt ttttgctgtt tagttaatgt aaagttgata atgtaattgt ttattccttc
    39781 tctattcata acgtgtttgc tcaaaatggt tatataaaca aaaatctact aagtgccatt
    39841 gaaaaggact tagtagattt attaaatata tgcgtagcaa cattattttt gtcttgccca
    39901 tctcgtgaaa ataatactac atataatgcc aaatatagta attgttataa gtaaataata
    39961 gtatggtggg gtatagctca attgaatctt tgttatattt ttaggtgctt taatgccggt
    40021 cataacacca tttacttgtt caactttaag actttgatca cctgatgtcg ctttcatacc
    40081 ttgattatat gctgttggca aaacaatata cccagatgaa tttttatttt tagtaatagt
    40141 ataaccatgc tttgtcttac taactttgac agcttctaat gaatttgaag cgtctttaag
    40201 cgtggtataa tcttcgccgt atatcccttt taaatttact cgatacttac ctttaggcaa
    40261 tgataatcta attctatctg gagctttaat gcgtatcgtt acgggtgtta caacgcgtcg
    40321 atatttataa gtgagtttat ttctttcttg tgtatattca ttcactttaa catcatgagc
    40381 tttatccggc gaaagtaatt ctaaatccat ttcaaaatac aaatctttaa attgattaga
    40441 aactgatttt ggcaactgta cagttagacc accattattt tgtttaactt gtaataaatg
    40501 ttttgtagga gattgccagg ctgcactatt taatttaatt gttgaatctg atagtaaatt
    40561 tttattggct ttaaaatgtg tattaacatc tttaatattg ttagaaacaa tcccttgcaa
    40621 cattgcttgt tctttatcta atggagattt taattcttta ttggaaaaga ccttatttgt
    40681 aatatgtgca cttggataat ggatggtatt tttagaatga atccaacgaa ctttattgtc
    40741 tttgtgttca gacttaattt taaatccata tggtaagttg tcatcatgat tcactctaat
    40801 tcgatcatta acattccaaa gtgatagtaa attttgacga ttgccaagta atctataagt
    40861 gctgttttta tcgattggca tattaatttg gagtgtcttg tcataatatt ttaaaatgtc
    40921 tccattaaaa atactagaat ataatgaaat gccattataa tgatatataa atggtgaatt
    40981 taatgcatag tctgacatat aatcaatgcg attaaatgag cctgttgcat tttgatttat
    41041 cttttttata agctggttta catagttact atggtaatca tgttgtttca acgttgataa
    41101 tgattgttga taaggtttga ttgccatgtt tttgttgtta tctaaaatga cgatttgttg
    41161 aatcataacg attaatacta atattgcaac ggttaatttt ttataacgcc ataaactaaa
    41221 ttttaaaata acggcaagca ctattagcag gataatacct actataagtg caagtgggtg
    41281 tgtcggtgat agtaatacat aaagtattgc gatgatgcat acgggtattg ttctgattaa
    41341 ataatatttc atatttaatg ttgataaatg ttgaataaac aatccgcaaa gagcacttga
    41401 tgatagtgct aagatataca cccaacgcct ttctggaaat gaaaaaccat taaaagcact
    41461 gtcgaaatac tgtgataatg aacctataaa taatatccat gttactattg cgaaaagtct
    41521 ataaaagtaa aaacgataca gtttgaatga caataatgca acgatagtaa gaattgaaat
    41581 cgtaatataa aatccatcgc taaagaaaaa ataatgataa tcaagtggtg tcaaaaacgg
    41641 tatatcaaca ttgggatttt gctttctgtc attttccaaa aacgcagaaa tgccagtgaa
    41701 taaaccaaat acacttgata acacactcaa aactgtagca gatatgacgc aaattaattt
    41761 ttgtgttcta gagacaatgt catatttgta agtgaaaatg agtcgatata aatagtagca
    41821 acctataata atagcttgat aataactgaa ataaaaattg ctaaatagtg ttaaggctat
    41881 cgcaacaatg aaaataccga ttttgcgttg ttgaaaatat ctttccaaac caagaatcga
    41941 taatggcaat aaatataata aatttccata aaatgaccaa gtaaaattaa agtatataac
    42001 gacagttgac atgccgtata aaatcgtagc gatcatattt gctgagcgtt taaagtgtaa
    42061 tattttaaat aagtagaagg tcacgacaaa tgttatgata gctcgtatca tagccataat
    42121 aagttggttt gtcggccaaa aatgtattgt cgtcggatta aatataccaa ccgtttctcc
    42181 tattttaatg aatagaaaat ttagccacat taaaggtgac agcgaataat aatatgatag
    42241 tcctttcata taatcgccac ctaatccaaa cgatgcatca tataaactag aaaaactacg
    42301 tagatgttca tacaaataca tttgaaatgg catcatttga cggaatccat ctccagcccc
    42361 gctaaaaaca gtaccattca caatataatc atagatatga gtagaaaata aaataagcgt
    42421 taatattaca ctaatgaaag ttataacaaa gaattgtttg acgtttgaat ttagccactt
    42481 ttttaacaca acattatcct caactttcaa atttaaaatt aagtttaact gaaactaaag
    42541 ttaatgaggt tcttgatagg taaagacgaa gatgactgtg gaacagatac cttatcatag
    42601 ttacttaaac tttggatcat tttcagttta tcattaaaca aatatattga ataataaaaa
    42661 tgtcatactg ataaagatga atgtcactta ataagtaact tagatttaac aaatgatgat
    42721 ttttaattgt agaaaacttg aaataatcac ttatacctaa atctaaagca ttgtt
//
LOCUS       DAMQYT010000006        41754 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMQYT010000006 DAMQYT010000000
VERSION     DAMQYT010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 41754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41754
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.006"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            289..1257
                     /locus_tag="PGA33_000235"
     CDS             289..1257
                     /locus_tag="PGA33_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDG6472076.1"
                     /translation="MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
     gene            1254..2810
                     /locus_tag="PGA33_000236"
     CDS             1254..2810
                     /locus_tag="PGA33_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDG6472077.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKD
                     NRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            2803..3561
                     /locus_tag="PGA33_000237"
     CDS             2803..3561
                     /locus_tag="PGA33_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDG6472078.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(3920..5299)
                     /gene="uhpT"
                     /locus_tag="PGA33_000238"
     CDS             complement(3920..5299)
                     /gene="uhpT"
                     /locus_tag="PGA33_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDG6472079.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            5532..6026
                     /locus_tag="PGA33_000239"
     CDS             5532..6026
                     /locus_tag="PGA33_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HDG6472080.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            complement(6388..7356)
                     /locus_tag="PGA33_000240"
     CDS             complement(6388..7356)
                     /locus_tag="PGA33_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDG6472081.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVSESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(7411..8451)
                     /locus_tag="PGA33_000241"
     CDS             complement(7411..8451)
                     /locus_tag="PGA33_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDG6472082.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            complement(8476..9552)
                     /locus_tag="PGA33_000242"
     CDS             complement(8476..9552)
                     /locus_tag="PGA33_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDG6472083.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRADSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            complement(9626..10465)
                     /locus_tag="PGA33_000243"
     CDS             complement(9626..10465)
                     /locus_tag="PGA33_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDG6472084.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            complement(10467..11735)
                     /locus_tag="PGA33_000244"
     CDS             complement(10467..11735)
                     /locus_tag="PGA33_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDG6472085.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYIINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            complement(11738..13009)
                     /locus_tag="PGA33_000245"
     CDS             complement(11738..13009)
                     /locus_tag="PGA33_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDG6472086.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            complement(13022..14119)
                     /gene="ugpC"
                     /locus_tag="PGA33_000246"
     CDS             complement(13022..14119)
                     /gene="ugpC"
                     /locus_tag="PGA33_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDG6472087.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            complement(14502..15080)
                     /locus_tag="PGA33_000247"
     CDS             complement(14502..15080)
                     /locus_tag="PGA33_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDG6472088.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGIQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            15289..15915
                     /locus_tag="PGA33_000248"
     CDS             15289..15915
                     /locus_tag="PGA33_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDG6472089.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            16105..16878
                     /locus_tag="PGA33_000249"
     CDS             16105..16878
                     /locus_tag="PGA33_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDG6472090.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGDMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNT
                     HKITGEYDTTTNDKKNATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQS
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPKNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKDQIIYKESLTYNKISEH"
     gene            complement(17176..19182)
                     /gene="ggt"
                     /locus_tag="PGA33_000250"
     CDS             complement(17176..19182)
                     /gene="ggt"
                     /locus_tag="PGA33_000250"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDG6472091.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVLEPFASGIGGGGYLLYYEQSTGSITAFDARETAPEHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLAKYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSTL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMYHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIAHGHAME
                     HKPDAYIGDVHGLQVDPTTYEASGGSDDTREGTVMGGEVLVIRKQPLPYRQMYDSDGF
                     RLYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFPEVSAHIEDLRSYENAGENYIDIA
                     WLARKYAYQVTLKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(19220..20995)
                     /locus_tag="PGA33_000251"
     CDS             complement(19220..20995)
                     /locus_tag="PGA33_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDG6472092.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSHDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            complement(21012..22175)
                     /locus_tag="PGA33_000252"
     CDS             complement(21012..22175)
                     /locus_tag="PGA33_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6472093.1"
                     /translation="MAQLNSKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            complement(22181..23659)
                     /locus_tag="PGA33_000253"
     CDS             complement(22181..23659)
                     /locus_tag="PGA33_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6472094.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     EQALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENVLFHKFI
                     MIKVILALPMFILLTFYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALMVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            23616..25208
                     /locus_tag="PGA33_000254"
     CDS             23616..25208
                     /locus_tag="PGA33_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6472095.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLNNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVIFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQTFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            complement(25473..26585)
                     /locus_tag="PGA33_000255"
     CDS             complement(25473..26585)
                     /locus_tag="PGA33_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HDG6472096.1"
                     /translation="MKICEKCFNNTEIVEIIANDNSKFDNCDIDNNHLGVKIFDTTKD
                     IDKLELIRDYLRPALELYDISINLPDTFSPKEGKKIEIALKDDWSIFNVEEAQISCIL
                     NELFKDDENLDRRVLEDLVGAKIINDKKYTNKNLIVANNDWDGFCESLKYKNRFHNNM
                     INLENLAFFLDITTNYYSIEEFRERFEPLYRSRIVKFITEKSELMSPPKGFATAGRLN
                     SKWISVLYLSTKEQVSIEEVKPKHNDIIYIGKVKLQKNVTKEKLKIANLTNLTSNAIK
                     AGDDGFRKYFVNYQTLKKIHKGITNPSDEQGIDYLPFQYLADYIRSLKYDGIMYESIL
                     QDGTFNFVFFDQSLFECVNYERKRVSDVKYTLTKLS"
     gene            complement(26572..27495)
                     /locus_tag="PGA33_000256"
     CDS             complement(26572..27495)
                     /locus_tag="PGA33_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDG6472097.1"
                     /translation="MYYPYLRGKQNELFAIRELLEKGLIGDCIQPIIEPIKYTTTFKN
                     TLQYCGEKAFSINLVVNSKLTEEEISNETVAHLTEIITKNKSVIQKAYLGPSDEGNDR
                     LKQQFSSNSLAILTSVDDWEMFGDKNKLEMVFVPDDRHIKRKLRNIPNKGIIMDPFNK
                     LSRNVDYLDNDDEFYSDDHLYYKEDGYVAFSDYSVIGGEYVDGGFSPLAIAIHIVYFD
                     EANELRVKHFVSDSNNDRSNPGKKFFEAVDKLVTWSKNLDIKNRSYALGQFEELNENN
                     KYPGLGLIKRLSIMHHLEIMNRYLESQNENM"
     gene            complement(27497..28339)
                     /locus_tag="PGA33_000257"
     CDS             complement(27497..28339)
                     /locus_tag="PGA33_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HDG6472098.1"
                     /translation="MDNRNMINRVFSQKILHQIAIKNKSDVVDEAYDFYIQGPKNINV
                     IQKMKSLYNYLKKSYRNEYFYKNTILNKLLLGRHSINTTTALSEMPIGKSIADFILLN
                     GKGVVYEIKTELDKLDRLDNQINDYYEVFNYVVVITNDKHLNKVMARYKDTTVGILVL
                     TTRNTLSEVQKPKENNSLLNTKAMYNFLRKEERKRVIAQNHMDVPTYNDFTEYDVLFD
                     VFKEIPMTKLHNNMISELKKRGNMKEYKDEFLAAPAEIKFLLYFAKMTKKDKNKLYHF
                     LKED"
     gene            complement(28544..31333)
                     /locus_tag="PGA33_000258"
     CDS             complement(28544..31333)
                     /locus_tag="PGA33_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDG6472099.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAPLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTTDIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTIAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            31546..31827
                     /locus_tag="PGA33_000259"
     CDS             31546..31827
                     /locus_tag="PGA33_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472100.1"
                     /translation="MVLITYQIILFFIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFVLVHLALKLFFSI"
     gene            complement(32021..32896)
                     /locus_tag="PGA33_000260"
     CDS             complement(32021..32896)
                     /locus_tag="PGA33_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDG6472101.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(32899..34353)
                     /locus_tag="PGA33_000261"
     CDS             complement(32899..34353)
                     /locus_tag="PGA33_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDG6472102.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            complement(34365..35261)
                     /gene="murQ"
                     /locus_tag="PGA33_000262"
     CDS             complement(34365..35261)
                     /gene="murQ"
                     /locus_tag="PGA33_000262"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDG6472103.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            complement(35261..36316)
                     /locus_tag="PGA33_000263"
     CDS             complement(35261..36316)
                     /locus_tag="PGA33_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDG6472104.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYDHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSDLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            36902..38947
                     /locus_tag="PGA33_000264"
     CDS             36902..38947
                     /locus_tag="PGA33_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HDG6472105.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            complement(38926..39027)
                     /locus_tag="PGA33_000265"
     CDS             complement(38926..39027)
                     /locus_tag="PGA33_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472106.1"
                     /translation="MMLQNTIAKKKQARHLMKVYAPVYSKHYLASNN"
     gene            39221..40861
                     /locus_tag="PGA33_000266"
     CDS             39221..40861
                     /locus_tag="PGA33_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HDG6472107.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQGGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTATKYTDASTYIELLATKLHQAKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNN
                     ATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMAVEDAPKKLIDIAKAFSQQNK"
     gene            40928..41485
                     /locus_tag="PGA33_000267"
     CDS             40928..41485
                     /locus_tag="PGA33_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HDG6472108.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPYTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
BASE COUNT        13443 a         7908 c         6341 g        14062 t
ORIGIN      
        1 acttttatat atgaagagac tggattaaat acttttattg acgtaaaaat tcacttttga
       61 accgttcaat atcttgccga tttttatata acagctacaa ataaaatata acagtttgat
      121 tttacagcct cggtaaatcg tcttgacaaa caaaaatttt gtgctatcac aacatttgca
      181 acgtcttaac aagtcatcta taaacatttc taaatattta acattactta tgcgtcattt
      241 attgctaaaa ttattgtatt aaaatataca tagaattgat gggatatcat gaaatcaaaa
      301 atttatatct tgctattatt tctcattttt ttatcagcat gcgctaatac gcgtcactct
      361 gaatccgata aaaatgtatt aacagtttat tctccgtatc aatcaaactt gattcgtcca
      421 attttaaatg aatttgaaaa acaagagcat gtcaaaattg aaattaaaca cggatctact
      481 caagtactgc tttcaaactt gcataacgaa gatttttcgg agcgtggtga tgtctttatg
      541 ggtggtgtgt tgtcagaaac aattgatcat ccagaagatt ttgttcccta tcaagataca
      601 tctgtaacac agcaattaga ggattatcgc tcgaacaata aatatgttac tagttttcta
      661 ttaatgccaa cagttatagt agtgaattca gatttacaag gagatattaa gattcgaggt
      721 tatcaagatt tattacaacc tatacttaaa ggtaaaattg cgtactcaaa tccaaataca
      781 acaacgacag gctatcaaca tatgcgtgct atttatagca tgcatcatcg agtaagtgat
      841 gtgcatcaat tccaaaacca tgcgatgcaa ctgtcaaaga cgtctaaagt cattgaagat
      901 gttgcaaaag gtaaatatta cgcaggtcta agctacgaac aagatgcacg cacatggaaa
      961 aacaaaggtt atcctgtatc aatcgtttat ccaattgaag gaacaatgtt aaatgttgat
     1021 ggtattgctt tagttaaaaa tgcacaccct catcctaagc gtaaaaagtt agtgcaatat
     1081 ttaacaagcc gctcagtaca acaacgatta gttgcagagt tcgatgccaa gtcaattcga
     1141 aaggatgtta gtgaacaaag cgatcagtct atcgaaaatc ttaagaacat acctttaata
     1201 ccgaaatcta aattaccgga tattccacat cataaatttt tggagatgat tcaatgacgg
     1261 catacaaacc ttatagacat caattaagac gatcgttatt tgcgtctact atttttccag
     1321 tatttttagt cattattatt ggactagtaa gcttctatgc catctatatt tggattgagc
     1381 atcgtacgat tcatcaacat gttgatgaaa gtcagtcatc tttacatcat actgaaaagc
     1441 agattcagac atttattaca cagcataata attcgtttca agagctcgat ttaacaaatc
     1501 atcatgatgt gaccgctaca aaacgtgaat tattaaaatt aatacaccaa cagcccgcaa
     1561 cactttatta tgaattatct ggaccaaatc agtttataac caataattat gaacatttaa
     1621 acactaaaaa tatgtattta ttttcaacgc atcagcttaa attcaaaaat agtacatata
     1681 tgcttaaaat ttatatggct aacaccccac gtttaagtga aattaagaaa gataaccgac
     1741 aattcgcact tatcgttgat caatatgaca atattttata cgccaacgat gaccgtttta
     1801 ctataggtga aaagtataga ccacaacaat tcggatttat gaatgaatct gtaaaattaa
     1861 accatgctga ccatcgtctc attatttata aagatataca tgaaaacatt gaagatggta
     1921 tcacgttgtt aattgttatg gccgtagttc ttgttttact agtaatattt ggtttcatta
     1981 gcgcagataa tatggctaaa cgacaaacaa aagatatcga aacgattatt caaaaaattt
     2041 actatgccaa aaatcgtcat ctaggtacat atacgccttt aaaaaacaac agtgaactag
     2101 aagaaattaa taattatatt tacgatttat ttgaatcaaa tgagcaactg atccattcta
     2161 tcgagcatac agaacgtcgc ttgcgtgata ttcagttaaa ggaaatagaa cgccaattcc
     2221 agcctcattt cctattcaat acgatgcaaa caatacaata tttaattacg ttatctccga
     2281 aattagcaca aactgtggtc caacaattat cgcaaatgtt acgttactca ttacgtacaa
     2341 actcacatac agtggaatta aacgaagagt tgaactacat tgaacagtat gttgcaatac
     2401 aaaacatccg ttttgatgac atgattaagc tccatattga aagttctgaa gaagcgcgtc
     2461 accagacaat tggtaaaatg atgcttcagc cactcataga aaatgctatt aaacatggta
     2521 gagatactga gtcattggac attactatac gcctgacatt ggcacgtcag aatttacatg
     2581 ttttagtttg cgacaatggt atcgggatgt cgtcatctcg cctgcaatat gtacgccaat
     2641 ctcttaataa tgatgtattt gatactaagc atttgggatt aaatcattta cacaataaag
     2701 caatgattca atatggttca catgcacgac tccatatttt ttcaaagcgt aatcaaggta
     2761 cgttaatttg ttataaaata ccactttcaa gggggaatgt agatgtttaa ggtagttatt
     2821 tgtgatgatg aacgaattat tcgagaagga ttaaagcaaa tcattccttg gggagactat
     2881 catttcaata caatatacac tgctaaagat ggtgttgaag cattatcact aattcaacaa
     2941 catcagccag aattagtaat tactgatatt cgtatgccac gtaaaaatgg cgtcgattta
     3001 ctcaatgata ttgcgcatct agattgtaat gtcatcatat tatcgagtta cgatgacttt
     3061 gaatatatga aagccggcat tcaacatcat gttcttgatt atttattaaa accagtagac
     3121 catgcacaat tagaagttat tttaggaaga cttgtccgta ccttattaga gcaacaatca
     3181 cagaacggcc gtagcttagc atcttgtcat gatgccttcc aaccactatt aaaagtagaa
     3241 tacgatgatt attatgtaaa ccagattgtg gatcaaatta agcaatctta tcaaacgaaa
     3301 gtgactgttt cagatttaat tcaacatatc gatgttagtg aatcgtatgc gatgcgaaca
     3361 tttaaagatc atgttggtat tacaattgtc gattatttaa atcgctatcg tattttacaa
     3421 tctctgcaat tgttagatcg acattacaaa cactatgaaa ttgcagacaa agtaggcttt
     3481 tcagagtata aaatgtttag ctatcatttt aaaaaatatt tacaaatgtc gccaagtgat
     3541 tattgtaagc aagcaaaata gattagcaca taactaatga ttatggcgcc tccattttgt
     3601 tctatgaaaa agcggactga tagagaagtt ttcacttttc tttcagtccg ctttttagag
     3661 agtttaaata taaaacaata ctccctacaa tattgacacc gctatatgtc tttattgtaa
     3721 ggagtaaatt cattgagtca ccctaagtcg ggcaaataag gatacttcat aacagggaat
     3781 tatgatctgc cccgagacat agagatgata tatttgcaag ggtagaagca gtatctttca
     3841 tctcttcacg cacatgccgt cggggcagga ttgccaacta tattgcaata gattggcgat
     3901 acttttaatc cgatttatat taaattttta aacttctaat tttcttttct tcatagaaag
     3961 caacgattcc taatagaatc atgcctagga ataatgcaac atagaagacg atgaaaacat
     4021 ctgtccatcc acttaatgta tatccaaaga tgtttaaacc gttacgtgtt ggatcagcaa
     4081 tagccgccaa accaactttc gccattgagt caccgaatag atacgcgaat gaacctgtca
     4141 ttccgtttgc tacactgatg gcatttttag gaacaaaacc agtcaatgat acaccaatta
     4201 ataattgcgg accaaagatt aacgcaccta atgcaaacaa tgaaatgtta accatcatga
     4261 cacttgtagc atttgtgtag aataagacaa caaatgtaat cataaacata cagccaatag
     4321 ctacaattgc acgacgacct tttaataagt ctgatacgta gccccataat aaacttgcaa
     4381 ctaatgcacc aatttcaaag tagaatatcg tattaactgc atcgccttta ctaaagtgta
     4441 aatgctctga cacatataac ggtgcccagt tatcaatacc gattcgtaca atgtatacaa
     4501 agacgtttga aacacataga atccatataa caggatttcc caggatatat tttttaaaga
     4561 tctcccattt cgtcatacct tgagaatcaa tattttcttt atcgaccggc tcttcccaaa
     4621 tttcttcagc acgattccat cctaattctt ccggatcatc ttttccgata aataatgttg
     4681 cgataccaat aagtaatgca atcaccgatg ggaaaatgaa catccctata acatttccat
     4741 ggaagaatac attagcaccc caaagtgcaa caccacctgc tatggcacca ccgatattat
     4801 gtgatgtatt ccagaatcct aagtatcggc cacgtttcgt tcttggcgcc catcttgaaa
     4861 tcgttgaata acttgcaggt ccaccaactg attggaacac cccgttaagt ccccaaagta
     4921 caattaataa tcccattaca gaaccaaagt aacttaaaac aaatcccata attaaaactg
     4981 taatcgcaga taagataagt aagaacgaga taatacgttt tgtgttacgt ccatcgacaa
     5041 aatatccaag taatgttttt cctaaaccgt acgtgatact aaatgctaat ccgatataac
     5101 caagttctaa tgtagataat ccaatttcct cttttaaaaa cggttgtgcc gccttaaagt
     5161 tgtttcgaat taaatacata gccatataaa caaagaacac tacgaagaaa gcttgcatga
     5221 agtttctaag ccataattta cgttgtaccg ataatggaat gcctttgttc ggaatcttat
     5281 ggatatcaaa aaagttcatc tctgtcacct caatcatttt cgttaagcca aatatatcac
     5341 gtcggtgtaa gcgcttaata tccttaaaat tattaaatta tccaaaatac tgaacataaa
     5401 cacatactta gaaacatatt tatgtactaa aattaataaa tagttaatag tatttgacaa
     5461 gctttatcta taaaatactt tatgttgtca aaatcaacgc ttatacatat gacataatga
     5521 cggaggacat catgattcta tctatattac ttatcttttt ctgtatcaga cttgtcagct
     5581 taaagatatc tattaatcac tcaaaacaat taaaagcaga cggtgcagtt gaatatggcg
     5641 ttaaaaattc gaagttttta gcaataacac acgttttaat ttatgtattg gctggtgtag
     5701 aggcatttat taataaagat acatttagct ttgcaaatgg tattggctta gttatattaa
     5761 tctttgctta tatcatgtta tttatggtta ttaaaacttt aggtggtatt tggacattaa
     5821 aattattcat tttacctaac catcctatta ttaaatctgg attatataaa attacaaaac
     5881 acccaaatta cttcttaaac atcattcctg aattaatcgg tgtattatta ttaacacacg
     5941 caacatatac aactatttta ttagtaccgt atgcgtattt cttatatgta cgtattaaac
     6001 aagaagagaa attaatgaat atttaaaaag atgaaagcct gtcatcactt tagtctacat
     6061 cgacgtccat ctttaaagtg cattctgaaa tattatattc agaaaccttt aaagtatgtc
     6121 gtgtctatga ccattgtgac aacaggcttt tttttattat catatacctc actattgaaa
     6181 aggttatata ctcttttcaa tggtgaggat tgttactgtg aatgggtatg accatcgcta
     6241 tatacttata taaagtacac cttctttaca aacaatgact tgatttaagg tgtgaaattt
     6301 ttggcaatgg cttggcgaca tatgcattta ttcaacactg ccaccagtct tacttcagac
     6361 aaacttcaga ctaacctcga gttcgtatta tgcccaccac atgtctgctg gcttgtcgta
     6421 aatattaaca gatttcaaag tttgacaagc tttttggaaa ccttcttcta ctgacataat
     6481 aggatcttca tgttcaatac ttaatacata atcatatcca ttaataataa gttgacttat
     6541 gatatctgcc catacatatg gactatgtcc ataaccaact gtacggaatg tccatgctct
     6601 tgtcgcaacg ttaccatatg gttgcatatc agttagacca tacatattta cattttcttg
     6661 attaatatac gtatctttag catggaagtg atgaattgca tttgcttggc ctaatatgcg
     6721 aatcgcagca attgggtcaa taccttgcca ccatagatga ctaggatcta agttagcacc
     6781 gatatattca tttgtagcct cacgtaactt caacattgta tatggtgtat gcactaaaaa
     6841 tcctgcatgc aactctatgg caatttttac atcttgctct tttgcaaact cagctaaatc
     6901 ttgccaatat ggtatcaact tttcattcca ctgataatca taaatttcag agtaggctgt
     6961 tggccaaggt gtaacaggcc aattaggctt tttagcggta tcatctgatc ctgcaatgcc
     7021 agaaaatgta ttaacaactg gcacgtctaa tagatttgct aaacggattg ttttacgtaa
     7081 cgtttcatcg gcttctttcg cttctatcgg atctggagaa attggattgt tatgacaact
     7141 gaaaccactt atttgtaagc ctctgtctgt gattgacttc ataaatgctt ggcgcttgtc
     7201 ttcattttct aacaactcat ctaacttaca aaatttatct cctgggttac cacctgttcc
     7261 aacttcaatc atatccaatc cagattctga gacataatct aacatatctt caaaattttt
     7321 atcgtaaaat aatactgaaa atacacctat tttcattgaa aagcactcct tctttttata
     7381 ttgagaattt tgtatcatat atgataatca ttaaagttgt atgctcttgt tatcaagatc
     7441 gctacgataa atcgcttcta taagggcatt tacattgcgt gcttcttccg gtttcaccac
     7501 aatctcttct atccctaaac acgcattgac aaagttacgc gcctgtctct caccagcaat
     7561 gtcttcgtta tgctcaacat tagctttgct ttcaaaaata gttccaaagc ggggctcata
     7621 tatttcaaat ggaaataaat tgataccgcc atcttctcct gataaactaa cgtgaacctt
     7681 atcttctttg atatttgcag accacgaaca ttcaaactgc atgcttgctc gattggcaaa
     7741 tgtcatataa ctagtaacat gatcatcgac atcaaattta gtatgatcaa atgttcccca
     7801 atcattaatt tgattcggtt gtttgctcaa ttgattatat gtttttccta gcacttcatg
     7861 cggcaccata tctttaccta gtagccacaa agataagtct aacaagtggc aaccataatc
     7921 gattaaacta ccgccacctt gcaacgcttt attggtaaaa acaccccagc caggcacttt
     7981 acgcctacgc atcgcttgta cacgtgctac taaaggttta ccaaccacac ctgattcaat
     8041 tgctttttta gcagtaattg ccacatctgt gtgacgataa tgatatgcga cagttaataa
     8101 tttgtgattt ttattagccg cttcaatcat gcgatcacac tcttccgtcg tcatcgccat
     8161 tggcttttca cacaatacat ggacaccatg gttcaatgct tctatagaaa gatcagcatg
     8221 gaatttatta ggtgtacaaa tgaccaccgc atcaacaagt ttaaacagct cgctaggtgt
     8281 ctcaactgca tgaggtatat taaagcgctt cgcaacatca atcatctgca ctgtattaat
     8341 atcttgtact gcaactaatg agactgtgtc tttgagtttc agcaatgctg gaatatgacg
     8401 gtcttgtgca ataccaccaa cacctatcac accaactttt aattttgtca tgatgtgcct
     8461 ccttaccgta tgatgttatt caaagtaaat tgctttgcct gattttgcag actgataaat
     8521 cgcttcaaga atttttgtaa ctaccattgc ttgttccggt ttcacaactg gttcagtatc
     8581 atttacaact gcatcaatcc aagcttttgc ttcttcttca gcttcatcca cttcattacc
     8641 ttcataaaag tcgacgcctt tgttttccaa ttcaacgtgt ttggtataaa gtgtacccat
     8701 gtcttcacca tgaatacgta gaccatcttt catatcagca cctgctttag ttcctgataa
     8761 tgaacatttt gcctcatcca cttctaaaga attaatcgcc caagcggatt ctaaaatgat
     8821 cgtcgctcca ttcttcattt taataaatcc aaacgcagaa tcttcaactg taaattcatc
     8881 tggattccat gaaccccaag cgtttgccgc atgatgctgt ttatttaatt tatggaatgt
     8941 tgaacccatc actgattctg gttcataatt atccatcatc cataacgtta aatctaaagc
     9001 gtgtgtaccg atatcgatta atggtcctcc accttgagct tcttcgtcta gaaagacacc
     9061 ccatgttggt actgctcgac gacgaatggc atgtgccttt ccgaagtaaa tgtctcctaa
     9121 gtcgccacgt tgcgctgctt gatgtaaaaa ttgactatct gctcggaaac gattttgata
     9181 acctattgtt aattttttac ctgttgattt agctgtatct atcatttttt gagcttctgc
     9241 tgtcgtttta gccattggtt tttcacacat cacatgttta ccagcatgca accctgctac
     9301 agtaatttca caatgcgagt catttggcgt acaaacatgg ataacatcaa tcgtgtcatc
     9361 ttttaacaat gctttgtaat catcataaac ctttgcattg tcagttccgt atgcttctgc
     9421 cgcactcgct gctttcgaaa tgtctacgtc acaaaatgcg atcatttcaa cattttcaac
     9481 tttttgtaaa cttggcatgt gcttgccatt cgcaatacca ccacacccaa tgattccaac
     9541 tttaatcgtc atcataacgt cctcctttta atctttatcg tcattagtgg ttctttatca
     9601 attctgcccc actcctaatt tcaaactaac cttttgtcgc acctgttgtt aaacctgata
     9661 ctaaatagcg ttgcaagaac aagaatacga ttgctatagg tactgcaatc ataattgccc
     9721 ctgctgcaaa cactgtgaaa ttatttgcat acttatcatt aataaagttg aacaatccaa
     9781 ctgctaatgt gaatttttca ggacttctta atagtatttt aggtaatata aagtccataa
     9841 atggccccat aaaattgaac aaagcaacaa ctgctaaaat cggcttagct aatggaagca
     9901 taatttgtaa gaaaatacgc atatgccctg caccatcaat tttggcagat tcatcaagtt
     9961 cttttggaat cgtatcgaag taacctttca ctaaaaaggc attcatcggt attgatccac
    10021 caatatatac cagtgttagt ccaaataaag aatctaataa tccaattgta tttagcaaaa
    10081 tatagattgc gaccattgcc attaatacag ggaacatttg taaaatcaaa aatgtaatca
    10141 gcccgtattt acgaccaaca aagcgatatc tagaaaaagc atatgctgtt aacgtgacaa
    10201 atatcacact aaacagtgca tttgcagatg ctacgataag cgtattttta taccaagtca
    10261 ggtattgact actgtcatcg aatagtaaga atgcataatt tttaaatgtt gcattgtctg
    10321 gtatcatttt ggcaccatac aagttcgtac ctggattaag ggaaatgcca aatgtccaca
    10381 gtagtggata taaaatgatg acaaacatca tcgctataaa actgtaaata ccgattgctt
    10441 ttaatatgtt tttcttcttt gtcatcttat aaacctccct catctttaaa cgtacttgta
    10501 cgtctgaatt gaataaatgc gacaatagca acaataaatc caataattaa tgacacaact
    10561 gcacccatgt tgaagttgtt aaactcaaat gtgagattat acacccaaga tatcaagata
    10621 tctgtactac cagcattctg ccctgacact ggtggaccgc ctttattaaa tagataaata
    10681 agattaaaat tattgaaatt acctgcatat tgcataatta acaatggcgc tgtggcgtaa
    10741 atgacatgcg ggaatgtgat gtttctaaac ttttgccaac tagacgcacc atccatatct
    10801 gctgcttcgt accaatctga tgaaatactt tgcagtactc cagtgaacag tgcaaagaca
    10861 aatgggaatc caagccatac ttgaatgccg attaatgcca cttttgccca aaacggatca
    10921 cttaaccatg ctggtgctac acctaataaa ggttgcaaaa tatcattatt tatcgcacca
    10981 aattcatcat taaataacgc tacaaatatt aaaattgtca caaatgatgg tacagcccaa
    11041 ggtaggatta acacagtacg gataaatttc ttacctttga cgacagggtg atttacaata
    11101 attgccaaaa acagccctaa tgcaatttga agtgtcgttg caacaagcgt ccatactaat
    11161 gtccaagtaa taacactgaa aaatgtttta cgccaaacgc caattgtgaa taacgtttta
    11221 aagttatcta aaccaaccca ttctaatgtg tgtctcggag gcgcgttgta taaattgtaa
    11281 tttgtaaatg ctactccaaa cataaatatt aatggaaata caactacaaa tatcaataag
    11341 aatgtacctg gtgagattag taagtatggg aacgtcttgt cccatgttgc caccatacgc
    11401 gccttcggat cctttatttc ctcattgcga ttaaatcgtt cagcattacg atatgcatct
    11461 aaaatattaa tgatatatag catgattgcg aaagcaacga gtaagataga aataatacct
    11521 tgtgcaagta agacacgaga atcgtctaac ttaggtactg tccctaatgt gaacaatccc
    11581 caaaaaccaa tatttaaaaa gctataaaaa acagaaataa aactgatgaa aaagataaaa
    11641 aatatcgtcc ctttaatggg tcttttatta taaaactgtc ccaaaccagg tataacagat
    11701 aataatgccg ctaatttagg gttacgtttc gtcataacta atctcctttc ttatcatttt
    11761 gtgatggatg aagaatctta atattttgcg ttatatcatt cgtcgcctca tctaacgctt
    11821 gtttaggatt cttaccattt gaaataaata tgcttgcatt gcccatcggt tcccaaactt
    11881 gtcgcatttc aggaatatta ggcatcggtt cagcatgacg tgcttgcttt tcaaacactt
    11941 ttaaatttgg attagatgat ttcacgtcaa cacgtccagt aatttcgctc atttcatctg
    12001 tatatttttg taatgtatct ttactagtga tatacagcat taaatcttta gcccaatact
    12061 tatgtttact atattcagat aaataccaac cacgtacacc tagaaatggt ttcataggtt
    12121 tgccaccatc tgtaggtaat gttgttactc ctaaatcttt accaaacgtt tcttgatatt
    12181 cattaatgtt ccacggtcca gtgacaaatt gtcctacttt tccttcttta aaaagaccaa
    12241 tcatgacatc atgtgttgct gccttaggaa gatacccttt gtcgtaccat ttttgtaatc
    12301 gttcagcatt cttgacgaca tgttttgaat ttagtcctag ctgatgaata tcatattcac
    12361 tgccattttt cttgaaaata taatcatcat tgccgaataa aaacggataa ttaaaataga
    12421 aatttttagc atcaaataac ataccgtatt gtttcttttt actatcagtt agtttggcag
    12481 cattagcttc aacttcttct aacgtttgcg gtgcattttt cactaatttt ttattataaa
    12541 aaagtgcggt tgtttcaacg atagctggca atgctagttg cttattgtca taattcatcg
    12601 ctttaagtgc ttgcttattg aaacctttca actcatcttt tgataatttg atttcagcag
    12661 ctaagccttg tagataggca cttccagtat tatcatgtgc taagaaaaag atatctggac
    12721 cttttcctgc aggagcgtct agcgaaatat tttctagttg atcattttga ccaatattta
    12781 caagctttac tttgatgcca gtttttttag tatattgatc cgtaattttt ttataaaacg
    12841 ccatttgctt gtcgccatcc acccacatcg taagttggtt aggcttgtct ttagaattat
    12901 ctttattcaa tgctttatca atatcttctt tcgaacgatt agggccacat gcagttacga
    12961 ttaataacat taccacggct aacgtgatac attttaaaat tttagacatg aatactcccc
    13021 cttagacgat acgatttcct gttttttcat caaaaaagtg acacttattc atatcaaatg
    13081 ctagtgtaat cttgtcgttc gccatcactt gagttcttga atctaattta gaaatcaatt
    13141 ccattccttg gaatgtgcta tgtaccataa tttctgaacc taacagttcg gatacaacta
    13201 cttcagattc aaattgtgtc tctggagaag tttgaataaa tattggttct tcatgaatgt
    13261 cttcagctcg aatacctaaa ataatttcct tgtctaagta gccagcagct tttaactttt
    13321 caaatttttt attatgtaat ttaaaatggt gtgttcctac cttcaatccg tccatttcaa
    13381 ctgtagcatt caacatattc attgctggtg agccgataaa ttgcgccaca aatatgcaat
    13441 taggggcatc atatatttct ctaggtgtgc cgacttgcat aatgtcgcca tctttcaaaa
    13501 caacaattcg actagccatc gtcaatgctt cagtttgatc atgtgtaaca taaattgtcg
    13561 tagtattaag tcgcttatgt aatttcaata tttctgtgcg catttgtact cgaagcttcg
    13621 catcaagatt cgataatggt tcatccatta aaaagacttt cgcatcccta acaatagctc
    13681 tgcccaaagc aacacgttga cgctgtccgc cagataacgc ttttggttta cgaccaagat
    13741 actcagttaa tcctaatatt tcagctgctt cattaacttt ttgttcaatc tcttttttat
    13801 ttactttacg tagctttagc ccaaatgcca tattttcaaa aacagtcata tgtggatata
    13861 atgcatagtt ttgaaatacc atcgcaatat ctctattctt tggttcaaca tcgttcatgc
    13921 gttccccatc aatataaaaa tctccagatg tgatagactc tagtccagca accattcgta
    13981 atgttgttga tttaccacat cccgatggtc caacaaatac aatgaattct ttgtcagtaa
    14041 tatgtagatt aaaatctttc actacagtat tgttgttatc atacgtcttt ttaatatgct
    14101 ctaactttag ttctgccatg ttatgcctcc catactttgt ttacagtttg attgtgcaat
    14161 acacttcgtg aaaacgtttg cacaaatact tacataaatc atatattcag tcattattaa
    14221 tgtcaatcat aaattataaa cgtttacatt actgtcagaa ttaaactttt atacagaaaa
    14281 ttgatatagt gcacaactat tttctagtgt gacaataatg ttttttatgt gcgatatttc
    14341 actgccaatg ctaacaacct tgtcttacat tttaagtaat atctaaatca aacaagtatg
    14401 acttagcatt acgattttca ttgaaaaaag caaccattat gtaagtatca tactcacaca
    14461 atgattgctt aattttttag actgcgggca cccttctata actacaaatc atataggcta
    14521 cgttccccat caggtaactg atcgataaac ggttttggat cttctgcata tgcattacct
    14581 acgccaccct tcattctttg aaaatgtaaa tgtgcgcctg tcgtttgtat acctgtattg
    14641 cctgaatatg caataatatc acctgctttg actcgatcac ctactttgac attatatttg
    14701 tctaagtgta gataccactg gtgatattct ccattgtctt cggcaatctg taataccttg
    14761 ccgcccaatt cattattaaa gatacgtgtt actttaccat tcgtcggcgc tttaattggt
    14821 gtaccttttg gcaatgcaaa atcaatgcca taatgcttac catcaaatgg tgaatgttga
    14881 tatttaccaa aagtctcagt aatccgactc cctctaaaaa agtcttccca ttttgagtgt
    14941 ccttgtcctt tatgattttg tgtcaattta tcggtatatt ggttaaacca tttttgaatg
    15001 tcatctatat aaataatcgt aaataatagt gaaagaaaaa tgataattgt tgccaaaata
    15061 cgttttggtc gctttgtcat agtggcatcc atccttcata tattttgggt attaagtata
    15121 tattctaaat caactatttc ataattaaat gcactcatac tttagtagta ctgccaaata
    15181 tagatgaaga aaatgatggt aatttgaata ttttgattga tattaaattt tatatcgagt
    15241 ataataatta tacttaagaa aataacagaa acagaggaga attttcaaat ggcaaaagta
    15301 ttatatatta cagcacaccc ttttaatgaa ctggtatcaa attctatggc ggctggtaaa
    15361 gcatttatcg aaacatatca acaacaacat cctgatgatg aagtgaaaca tattgattta
    15421 tttgaaactt atattccagt tattgacaaa gatgtattaa ctggttgggg aaaaatgagt
    15481 aatggcgaaa cgttaactga tgatgaacaa atgaaagttt caagattgag tgacatttta
    15541 gaagaatttt taagtgcaga taaatatgta tttgtaacgc ctatgtggaa cctttctttc
    15601 ccacctgtag ttaaagcata tattgatgct atttcaattg caggtaaaac ttttaaatat
    15661 tctgctgaag gaccacaagg tttactaact gacaaaaaag tattacacat tcaatcacgt
    15721 ggtggatatt atactgaagg acctgctgct gattttgaaa tgggtgaccg ttatttaaga
    15781 acaattatga cattcttagg tgtgccatca tatgaaacaa tcattattga aggacacaat
    15841 gctgaacctc ataagactga agaaattaaa gcaactagta ttaataatgc tgaaaaatta
    15901 gcaacaactt tttaaatttt tagttacact gtgtgacgtc tcaaacgtca tgcagttttt
    15961 tattgtatct cttttccgct ttttataaat atctattatt ttcataaatt taaaatttga
    16021 tattcacttt aatactgatg taaatatttt tcaaaatata ttattatagt gttaatgaag
    16081 tattttgaga aacggtggga atgtatgaaa gcacataaaa ttttctggtt aaacttagca
    16141 gcaattatta ttatttcaat tgtagtcagt ggcgatatgt ttttggcaat gaagtgggaa
    16201 caaattcatt tgaaagatgg tcttaaaaaa gtactaagta cgtatcctat taaaaactta
    16261 gaaacgcttt atgaaattga tggtcacgat aatccccatt atgaaaataa tgatcaagac
    16321 acatggtata tagaatcctc ttattcagtt gttggatcag atgagctttt aaaagaagac
    16381 cgtatgttgt taaaggtaga taagaacaca cataaaataa ctggtgaata tgacacaact
    16441 acaaacgata aaaaaaatgc tactgactcg acatacaaaa gttatccagt aaaagtagtt
    16501 aataataaaa tcgttttcac aaaagacgta aaagatcccg cgctgaaaca aaaaatcgaa
    16561 aacaatcaat ttttgattca aagtggtgat ttaacaagca ttttaaatag caatgatttg
    16621 aaagtcacac atgatcctac cactgattat tataatttat ctggtaagtt gtcgaacgat
    16681 aatccaaacg ttaaacaatt aaaacgtaga tataatattc ctaaaaacgc atcaacaaag
    16741 gtggaattaa agggaatgag tgatttaaaa ggcaataatc atcaagatca gaaactttat
    16801 ttttattttt caagtcctgg aaaagaccaa atcatttata aagaaagcct tacttataat
    16861 aaaataagtg aacattaata cttatgctgt aattatagaa acatccaaat catctattaa
    16921 aatcctatat tataaaaaca cctcacataa ctcgttcaac tgtaccaaac cacattacat
    16981 tagattttag gctaactatt gtgatgtaca tcaaaaacga atttgtgagg cgttgtatat
    17041 tttacaaagg tgactagcgt ttcgtatagc atttccaaca ttactacact caagcgtcac
    17101 gctaaagttc gaaatcgaat cctttcattc aacaaaagct catatccact acaaacttca
    17161 tatcaagcgt ataaactatc ttgtgatact atctcgatca tatctatagt atgcatttgt
    17221 gttccgtttc actgaagtat atgtatcatc agttaagtat aaaccgtcat ccttcaatgt
    17281 tacttgataa gcatatttcc gtgctaacca ggcaatatct atataatttt ctcctgcgtt
    17341 ttcataactt cttaaatctt caatatgtgc actaacttca gggaaaatga ttctaacaac
    17401 actttcatca acccaatatt tgtcatgcat ccatcgcact tgatctgcca ataaaggtaa
    17461 ctgcacatca ttgaaatata gacgaaagcc gtcactatca tacatttgcc gatatggtaa
    17521 tggctgtttt ctaatcacta acacctcgcc acccattacg gtgccttctc tagtatcatc
    17581 acttccaccc gaagcttcat acgttgttgg gtcaacctgt agtccatgta catctccaat
    17641 ataagcatct ggtttatgtt ccattgcatg tccatgtgca atcaatgcta atattgtaga
    17701 ttgtgaaaat tgaggctccc attcaatgcg attaggatgg ctactataaa ttctaggttc
    17761 atctatagcc tgctgaatat ccatgccaaa cactaataca ttgattaatg tttgcgcaac
    17821 actagcaatg atacttatgg caccaggtgc acctactgtt aatattggct tcccgtgata
    17881 catcacaatc gttggagcca tgttacttag tggtcgttta tatggtgcaa tttcgttaat
    17941 accaccatct actacatcaa agccatccat tgtcgtattc aataacacac cgtagcctgg
    18001 aatcgtgata cctgaaccat aaatcatacc aattgatgtc gtaaatgaag caatattacc
    18061 ttccttatca atcacacaaa aatgagttgt ttcggtatga ttttcttcaa catctgtatg
    18121 actaatgcaa tcagacacaa caccatgctc tatatcaata tttgctttat tgctatcaat
    18181 gagcgtactg cgtgctttta aataatcatc atcaattaat gactgtacag gcacctcatg
    18241 aaaattatca tccgccaagt attgcgcacg atcactatat gctaaatgca tcgcttgtat
    18301 caaatgatgc aagtaatcaa cagatcttgg acccatagat ggtaaatcga catgttctaa
    18361 taacttcaat atttgaatta ccgtgatacc gccagaacta gatggtccca ttgaataaat
    18421 gtcatagtct ttaaatgttg cactgattgg cgctttaatc tgaatgtcat atttggctag
    18481 atcctctaaa gtgattgtcc caccacatgc tttgacaaca ttgactaatt gtttcgcaat
    18541 gtcaccttta taaaatgcat taaacccttg ttctcttaat atttgaaatg tcttacctaa
    18601 ttcgggttgt acaatccaat caccttcacg ccaatattga ttttcatgcg taaatacttg
    18661 tgccgtttca tgatactttg tcaatcgtgc gtgttgctgg cgcgaatatt tttcagtagc
    18721 ccaattggct gcatgacctt caatggctag ttcaattgca ggattaatta aatcttccaa
    18781 tgacaattta gcataacgct tgtgaatata atcaaacagc tttggaattg ctggcacagc
    18841 gacagtttta ccatgtgtag tcatatcaaa aaatgattta tattcgcctg aatcatctag
    18901 ataaaattgt ttgtctacat gttcaggtgc tgtctcacgt gcatcaaacg cagttatact
    18961 gccagtactt tgctcataat atagcaaata cccgccacca ccaatacctg atgcaaatgg
    19021 ttctaacaca ttcaatgcca gttgaattgc aatcactgca tccatggcgt tgccaccttg
    19081 atctaataca tccttaccaa ttttagccgc aagaggatgt gatacggaaa ttaacccttc
    19141 tttagatgtt tttgtctgtt tgtcatttaa gttaatgacc atactatatc ctcctacttt
    19201 ctgttaaata tttaaaacat tattgattaa tggctttttc tactttttct aaatcttgac
    19261 gttgctcgtt accagtatcg acaagtggtg taatcggtga tgcaatttta aatttatcgc
    19321 cacgataaaa cttaataaat tgatcctgat ctatcgcatt aactactgct tgtctcaagt
    19381 ttggatgcgt cttaaatata ccttttttaa tatttagcat taaaaagact gacttgcgtc
    19441 catttttgcg aataatgctt aaatttttat ccgacttaat taaatcaaaa tgtttttgat
    19501 tcacatctgc caacatatca attgaatgat ttctaagttc tgacaatgca ttattcgggt
    19561 caccattaaa cttcaatgta atatttttaa ttttagctgg tccataacta cctttttctg
    19621 tttcgttgaa tcctggatta cgttgaaacg ttgcttgata tgcatttttc tgtgtcataa
    19681 tgtatgcgcc acttgcatac agcgcatttt tcccatctga atttgcagga attgtactgc
    19741 tatccccata tccttttgga tattcttgat ttacttgatt aacaaatttt ttagataaaa
    19801 tgcctgccga agagtgtgtt aagtaattta cctctcgagg catcgattga tctgtcgtaa
    19861 ttttaacaat ttgataaata ccgtctttat tatttacttt ttgaccatct gtcgttaacg
    19921 atttgacgtt ataagcttta atcaacttat catagattga tttatcgtcc ttgtctttct
    19981 ctttacgcaa ctgatcgatg tcctcatctt ttaatatctt gatgtcattt atatgtttgt
    20041 gcatattgta agtattattg ttaggcacag actttttatc acgtgctcta tctaaagaaa
    20101 acttaacatc ttcagccgat acacgctctc cagtattacg tgcttgtcca ttgaccactt
    20161 tcgcaaaata atcatcatct cttaacaaga aataaaatgc tttattgtcc ttattcacag
    20221 cataatcatg acttaacgaa cctttcgttg ttaaatgatc attttcatct aataataata
    20281 accttgtgta catattcata ttaattgaat atactgacgg cgcaattgaa cgtattggat
    20341 ccaatgtagg aatttcacca tcttgttgtg tcatcacaag tggccgcgta tctcgttctc
    20401 tactattgtt gtaatcaaat tgttgccata ttaatgcacg tgaatttggc aatccaacac
    20461 tatttttatc taacacttta ttgtcatata ctaaattctt ttttgatcca tataaaggcg
    20521 ccatataccc tttatcaaat acaacttcat cttcaatttg cttatatgtt tgtttaacat
    20581 ctgcttcatt ttgagtagaa gctttattta acaactggtc tacatgttta tctttcaata
    20641 aactatttga tcctgtagaa ctaaataatg ccgtcatagc atagttcggg tcaccaaaca
    20701 ctgtcatcca gtcatcaatt tggatatcat aattgccggc ttgacgttgt gtacgatagc
    20761 taccataatc tggttggata ttcatcttca cgttaaatcc tgcattttcc aattgatctt
    20821 taacgatatt catatcattt tcataacttg cttgtcctag gaaatgtatt gttggtcgct
    20881 cgcctttcac ttcaactttc gatgactttt gagccacttc tgatttcgta gggacaccac
    20941 aaccacttaa taccaacgct aaaactataa ttgcgatact aatgattttc ttcacatcta
    21001 tccctacctt tttaatgaat tcttggatct agtgcatcac gcactgcatc acctataaaa
    21061 ttaaatgcta aaacgacgaa cataatacaa acaccaggta caatagctaa attactgtgc
    21121 gtttccaagt agttactacc ggtacgtaaa atgttgcccc attcagctac atcaggtgca
    21181 acaccaagtc ctaggaaact taaactactt gttgttaata caaccacacc tatatttaat
    21241 gaaaaacgta caatcatagg cgcaatcgca ttcggtaaaa tataacgcca tatgatattc
    21301 caagtgtttt caccagtgat acgtgctgca tctacatatt ccatgcgttt aatttctaaa
    21361 acactggcac gcattgtccg tgcaaatgat ggtatattac cgatacttaa agcaataatt
    21421 aaatttggaa tacttgctcc aaatgatgca ataattgcca ccgctaacaa taatgatgga
    21481 attgcaaaca ctacatctaa aattcgcatt attaaattat caatatgatt aaaataacct
    21541 gcgatagtgc ctagtaacac accaaaaata actgcaataa ctactgaaat aattgaaatt
    21601 gaaaatgtca gcttcgttcc tacaactacg cgtgtaaata agtctctacc gaaatcatca
    21661 gtaccaaacg gataggctag actcggtcca tgtaacagtg cattgaactg atttttagta
    21721 gccaatgtcg tatcaaatgt aaattgtgac acaattgata atgtcagcat gtagactaaa
    21781 ataagtaacc cgataatcgc aatacgatgt ctagtagttt ttcgtataaa cgattcccac
    21841 ccgttataac tatgtatttg cgatgtacgt tggtaacgtc taatacttac aaacattaat
    21901 aatgtaaata cgttgcctgt taatgtcatc aacaataaca acacttcgac gatacgtcgc
    21961 cataggtcat gatgcttcca tgtttgttcc gttgttaaaa taataattaa aatgatggtt
    22021 aaaacgatta gcaatgtttc agcaatatag aacgtatcgg ccacataacc tttaaaaaga
    22081 tttaatgcac tcgttaatat aactaaaata taagttgcta tggcgtaact tgcgaataat
    22141 tttaaggaag ctatctttga attaagttgt gccatatgcc tcacttcctt tcgttgattt
    22201 cactacgtaa ttttggatcg attaaagcat aaaatatatc aataattaag tttgctaaag
    22261 atattacaat tgatatatat acgaccccac ccatgactgc tggaatatca ggtattagtt
    22321 gtttttggac gatataacgc ccgataccat taatgttaaa tacttgttcc gtcactgctg
    22381 aaccgcctag taactctgcc actagaagac caactaacgt tacaattgga ataatggcat
    22441 ttttcaaaat atgtttaata acaacttgtg tcgtcgataa tccttttgca taagcagtta
    22501 aaacataatc gctgcgcatt acttcaagta cagaagacct tgtcatacgc gtgatagaag
    22561 cagcaatact tgttccaatg acaagtacag gtaaaatcaa cgatattgga tgttctggca
    22621 tataagatgg tggcaaaata tccaatttca atgagaacgc taaaatgaat aatagccctt
    22681 gccagaaact tggaatagat aaaccaatta atgcaattat cattaacgtg atatcaagcc
    22741 aactatttcg cttcatcgca ctgataatac caattggtat tgcaataatt aatgccacca
    22801 ttagcgctaa tactgcgaca attattgtaa ttggaattct ttcgccaact gctttagtca
    22861 caacctcatt ccctttgtaa gtcgtaccta agtcaaaggt aaaaacaccc ttgatggtat
    22921 cccacaattg aataaaataa ggttcgttaa gatgatgtaa tacattgaat tgatgtatct
    22981 gtgcctttgt tgcattttgt cccagtatgc tataagccgc atcaagcggt gaaaaataca
    23041 gaatggtaaa cacactgaca ataacaccaa tgatgacaat cacagccatg acaattcgtt
    23101 caaaaatata tctaactaat ggctgtaaat aaaaagtcaa taagatgaac atcggcaagg
    23161 ccaatatcac tttgatcatg atgaacttat gaaataatac attttcaaag tatgttgaaa
    23221 aatgtgcttg ttcaatattc tttgaactcg tattagaact ttgtgccttg aatattttta
    23281 atgcttcttt atgtatttgt gtggatgact tttgctgcga taaatattta tatttttgat
    23341 gtaacgcctg ttcaatttct gaaatttcag aattattagc gtaaaaattt ttcctcttag
    23401 cagaaaagaa aaactttatc actgcatata aaaatattgg caagcttaat accgataata
    23461 caaacttgta aaatgccttg tgatacaacg acttaaaaga ttctaaataa gtaattatga
    23521 taatcactcc ttgtaaaata atcttgagta gattactatg atatacaaaa gtatagaata
    23581 aatttacaca tttgtgaata gggaggcaca acatcatgtc aaatttatta gaagtcaaca
    23641 gtctgaatgt acaattcaat tatgatgaaa ctacagttca agcggtaaaa aacgtctctt
    23701 tcgaattacg aaaaaaacat atcctaggta ttgttggtga atcaggatca ggaaaaagta
    23761 ttaccgctaa atctatttta gggctactac cagattatcc agatcacaca ttaacaggag
    23821 aaattatttt taatgggcaa tcgttaaata atttatcaac ttcagcgtta caacaaattc
    23881 gaggtaagga tatttcaatg atttttcaag atccactctc ttcgttgaat ccaagattaa
    23941 cgattggcaa acaaattaca gaagtaatat ttcaacataa acgtgtatct aaatctgaag
    24001 caaagtcgat gacaatagac attttagaaa aagtaggtat aaaacatgca actcgacaat
    24061 ttgatgctta tccacatgaa ctttctggtg gtatgcgtca acgtgtcatg atagcaatgg
    24121 cattgatttt aaagccacaa attttaatcg cagatgaacc aacaacggca ttagatgcca
    24181 gtacacaaaa tcaattactg cagttaatga agtcccttta tgagtacaca gaaacatcta
    24241 ttatttttat cactcacgat ttaggcgctg tgtatcaatt ttgcgacgat gtgattgtaa
    24301 tgaaagatgg aagtgtcgtt gaaagtggca cggttgaaag tatttttaaa tcgccacaac
    24361 atacctatac aaaacgctta atagatgcga ttcctgatat tcatcaaacg cgtccgccaa
    24421 gaccgttaaa caatgatatt ttattaaaat tcgatcgcgt gagcgtggat tacacatcac
    24481 cgagtggcag cctataccga gcagttaatg atattaactt ggctattaga aaaggcgaaa
    24541 cattaggcat tgtcggtgaa tcagggtcag ggaaatcgac attagctaag acggtcgtcg
    24601 gtctaaagga agtgtcagaa ggctttattt ggtataacga attaccatta agtttattta
    24661 aagatgatga attgaaatct ttacgacaag agatacaaat gatttttcaa gatccattcg
    24721 catctattaa tccaagattt aaagtcattg atgtgattaa acgaccacta atcattcatg
    24781 ggaaagtcaa agataatgat gacattatta aaactgtcgt atcgttgtta gaaaaggttg
    24841 gcctagatca aactttctta tatcgctatc cacacgaatt atctggtggg caacgtcagc
    24901 gtgtaagtat cgcgagagca cttgctgttg aacctaaagt gattgtttgc gacgaggcag
    24961 tgtccgcttt agacgtttca attcaaaaag atatcatcga gttattaaaa caattacagt
    25021 tagacttcgg catcacttat ttattcatca cacatgacat gggtgttatc aatgaaatat
    25081 gtgatcgcgt tgcagttatg aaaaatggcg aaatcgttga actgaataac acagaagata
    25141 ttatcaaaca tccgcagtca gactatgcaa agcaacttat ttcagaagta gcagttattg
    25201 ctaaataaaa gtcatgcgtt gtgcaacttt atcactgtat ggtctgaaat aaattgcgcg
    25261 acttctgatg tgttatgtta gatgactttg tcatactaac tatattgtgc taagttaggg
    25321 ctcggcacca tatcaagttt taggtgcttt gccatgattt aagagtcacc cccatacttt
    25381 gggcatttta acgccagaat aaatcccccg ccactatgtg aagtgcgggg gattatttat
    25441 attttattag aatattcaga tttttgagtg tgtcaactta gcttagtcaa tgtatattta
    25501 acgtcactta ctctttttct ttcataatta acacattcaa ataaactttg atcaaaaaac
    25561 acaaagttaa aagtaccatc ttgtaatatg ctctcataca ttatcccgtc atatttaagg
    25621 cttcgaatat aatcagctaa atattgaaat ggcaaataat ctattccttg ttcatcgctt
    25681 ggatttgtta ttcctttatg aatctttttt aatgtttggt aatttacaaa atactttcta
    25741 aatccatcat cgccagcttt gattgcatta ctagttaaat tagttaaatt cgcaattttc
    25801 aatttctctt ttgtcacgtt tttttgtaac ttaaccttac ctatataaat aatgtcatta
    25861 tgcttaggtt taacttcttc tatactgacc tgttcttttg tactaaggta taatacgctt
    25921 atccatttag aattcaatct tcctgccgtt gcaaatccct ttggtggtga cattagttca
    25981 cttttctctg taatgaactt aactattcta gatctatata atggttcaaa tctttctcta
    26041 aattcctcaa tactatagta attagtagtg atatcgagaa agaacgctaa attctctaaa
    26101 ttgatcatat tattatgaaa tctattttta tacttcaagc tctcacaaaa tccatcccag
    26161 tcattatttg ctacaattag atttttattt gtatattttt tatcgtttat gattttagcg
    26221 cctactaaat cttccaacac tcgtctatct aaattttcat catctttaaa aagttcattt
    26281 aaaatacaac ttatttgagc ttcctcaaca ttaaatatac tccagtcgtc ttttaatgct
    26341 atttcaatct ttttaccttc ttttgggcta aaagtatctg gtaaatttat actaatatca
    26401 tataattcta atgctggtct taaataatct ctaataagtt ctaatttatc tatgtcctta
    26461 gtcgtatcaa atattttaac accaagatga ttgttatcaa tatcacaatt gtcaaatttg
    26521 ctattatcat ttgcaatgat ttctacgatt tcagtattat taaaacattt ttcacatatt
    26581 ttcattttga gactccaagt atctattcat aatttctagg tgatgcatga tagataacct
    26641 tttaattaaa cctaatcctg gatacttatt attttcattt aattcttcaa attgtccaag
    26701 cgcataagat ctatttttaa tatctaagtt ttttgaccat gttactaatt tatctacagc
    26761 ctcaaaaaac tttttacctg gatttgatct atcattatta gaatcagaga caaaatgctt
    26821 aactcttagc tcattagcct catcaaaata gacaatatgt atcgcaatag ctaatggcga
    26881 aaagccaccg tctacatatt ctccacctat aacagaatag tctgaaaatg ctacgtatcc
    26941 atcttcctta taataaaggt gatcgtcgct ataaaactcg tcatcattat ctaaataatc
    27001 aacattacga cttagtttat taaaaggatc cataatgatg cctttgtttg gaatattacg
    27061 caatttacgt ttaatgtgtc tatcatctgg tacaaaaacc atttcaagtt tatttttatc
    27121 tccaaacatt tcccaatcat ctacacttgt taaaatagct aaactattac ttgaaaattg
    27181 ctgtttcaac ctatcattgc cttcatcaga aggacccaag taagcttttt gaataacact
    27241 tttgtttttt gttattattt cagttaaatg tgcaacagtt tcgttactaa tctcttcttc
    27301 agttaacttt gaatttacta ctaaatttat agagaatgct ttttcaccac agtattgcaa
    27361 agtattttta aatgtggttg tatatttaat tggttcaatt ataggttgaa tacaatcacc
    27421 aatcaaacct ttctctaaca attctctaat tgcaaatagt tcattttgtt tcccacgcaa
    27481 ataaggataa tacatattaa tcctccttaa gaaaatgata tagtttattt ttatctttct
    27541 ttgtcatttt tgcgaaatat aacaagaact taatttcagc cggcgctgct aaaaattcat
    27601 ctttgtattc tttcatgttt cctctttttt tcaactcaga aatcatattg ttatgcagtt
    27661 tcgtcattgg tatttcttta aacacgtcaa ataacacatc atactctgtg aaatcattat
    27721 aagttggcac atccatatga ttttgtgcaa taactctttt tctttcttct tttcgtaaaa
    27781 agttgtacat cgcttttgtg tttaagagac tattgttttc tttaggtttt tgaacttcac
    27841 tcagtgtatt tctagttgtt aacactaaaa ttccaactgt tgtatctttg tatctagcca
    27901 taactttatt cagatgtttg tcatttgtaa taactacgac ataattaaac acttcataat
    27961 aatcattaat ttgattatct aatctatcca gcttatctaa ttctgtttta atctcataga
    28021 caacgccttt gccgtttaac aatataaaat cagcaatact tttccctatg ggcatctcag
    28081 aaagtgcagt agttgtatta atagaatgtc gtcctagaag gagtttatta agtattgtgt
    28141 ttttgtaaaa atattcgtta cgataagact ttttaagata gttatataaa gatttcatct
    28201 tctgtattac attgatattt ttaggtccct gtatataaaa atcatatgcc tcatcaacaa
    28261 catcactttt atttttgatt gcaatttgat gtaatatctt ttgactaaaa acacgattaa
    28321 tcatatttct attatccata gctacgcacc tccttcaaca tacatttaat acttatcctg
    28381 ttaatattat aaactatttt aaatgcctat agacattatt tctatacaca tacgattata
    28441 aaattgtatt gaaaccaaat gatggttgcc aaacacatgg taactaacaa aagatagaaa
    28501 ataataatgc cccctacact aatctagcga gggggctgaa tcattacaca ccgtattttt
    28561 cagttgtttc tgccacgaaa gattcaatgg cttttcttgc tttacgcttt tctttcaatg
    28621 gcaaatcacc aatcattttt ttaaggtgat gtgggtttac aacaccacta tactggtagt
    28681 catttgaaat tgtttttagg gcttgttcat cgatagatct ctctccagca aatcctttga
    28741 actccgcttc ttttttaata ctttcgaaat taacatattc ttgatcgata tcatcatcct
    28801 tatttaaaga aggtacaaca ttgtcgatga attctctaat tagatctcgt tttaacctca
    28861 atgtcggatc atctgcatga tctaaaatgc gtctaatttg ttcttggtta cgacgttgtt
    28921 ccgctttgtc ttcaagatca atttgtctca atatattcat aatataattc acattaatcg
    28981 tatcattacg catcatttct atttcgaaat caatatcatt taaaatggat actttatttt
    29041 tctcagccgt cgctcttttt acttgatcgt acacagctaa atatttactt ttatagtctt
    29101 cattctcttg ttcatccatt ccaatttcat caattgtaaa ctcaaactcg tcaaatgctt
    29161 ttaaacgtaa tattatttta gctaataaac gataagcttc aacaaagcgc tttagctctt
    29221 cttcatcttg aatgtcatca accatgtgtg gtgtcggcac aatcatttta agctcacgat
    29281 aagcgtccat aaattctttt ttatactctt cataactgcg cattaaaatt gtatccgtat
    29341 catttgtttg tgagaatact ctcagtgcat cgtctgtctc ttttttcaag tcacgatagt
    29401 ttacaatttt accaaatggc tttgattctt tttcaaccct atttgtacgt gaatacgctt
    29461 gaattaaatc atgatacatt aaattcttat caacatataa agtgttcagt actttactat
    29521 caaaaccagt taagaacata ttaacaacga ttaagatatc aattttacta tctttaacgc
    29581 cctttttaac gttttttgaa atatgattaa aatactcatt agttgtgtct gttgaaaaat
    29641 tcgtctcgaa ctttttatta taatcactaa tcattatctc taatttttca cgtgaatgat
    29701 atggcacttc accatcacga tcatcttcat taggtttaaa cgtaaatata ccagctatcg
    29761 ttaacggttg ttccaacttt ttgttaagtc gcttaaatgt ctcataatat ttaataagcg
    29821 cgtgaatact ttggactgta aatatacttg aatattgacg attacgtgta tatttatcat
    29881 gattattgat gatatgtcgt gttactaatt ccacacgttt atccgctaac catacttctt
    29941 ccgtatcaat tgcttcaacc atgctgttat cttctgcttt taaagcttta tttttaaaag
    30001 tattaatata gtcaactgag aaaccaagta cattaccatc atgaatggca tctctaatta
    30061 aatacgtatg taagcatcta ccgaaaatat ctgtagttgt tctaccatct tgactactat
    30121 tttctggaaa acgtggcgta ccagtgaatc caaagtattg ggcatttttg aaatgttgtt
    30181 taactagacg atgcatgtca ccaaattgac tgcgatgaca ctcatcaata ataaatacaa
    30241 ctttattcgt tttatactgt tctaataaag gggcattccc ttgaatcgct ttagccattt
    30301 tttgaatcgt cgttacaata agtggcaaac ttttatcatt tagttggcgt accagttgcg
    30361 aggtattaaa agttttgtct acagcaccct tagcaaattt attaaattcc tcttctgttt
    30421 gactatccaa gtctttacgg tcaaccaaaa agataacttt cttaatgtca tcttgctgtg
    30481 ataaaatctg actcgcttta aaagaagtca acgtcttacc acttccagtt gtatgccata
    30541 catatccatt attccctgtc tcagtcgctt gttgaataag tgcttctacc gcatacactt
    30601 gatacggacg cattgccatc agtattctat ctgtttcatt aataatcata tagcgcgata
    30661 tcatcttagc taattgacaa ggtctcataa atgactcagc aaacgattgc aatgtattga
    30721 tacggttatt ctgtttatca ctccaataaa acatgtgact cttcaatagt tcgctatcat
    30781 tattagaaaa gtatcgcgtt tcaacaccat tactaatgat aaacatttgt atgtagcgga
    30841 ataagcctgt gtaattttgt ttgcggtaac gttttacttg gttaaacgcc tcattaatat
    30901 caatacctcg acgtttcaat tcaacttgga caaggggtag tccgttgatt aatatcgtta
    30961 catcataacg tgctttatat gtatcctcga cagatacttg attcgtcact tgaaacttat
    31021 ttttacacca acttttcgta tctaaaaacg acaaataaat ctcagactca tcatcacgtc
    31081 taagtggtaa tttatcacgt aaaatacggg cactctcgaa aatacttttt ccatcaatca
    31141 tcgttaacag acgttgaaat tctttatctg ttaagggatt gccttctaat ttgtccgcat
    31201 gacgctcatt taaaatcgtt ctaaaattat caagcaattg cttattatca cgtatcgtta
    31261 ctctttcgta acccaattgt tcaagttgat tcatcatttc attttctaat gcgtattcac
    31321 tttggtatgc cattcatatc cccttccata cactttctat tgctctaaat atatcataaa
    31381 ctttaatgaa aaatgtttgt tttttatctt caaacgtaaa tttattctaa ttttattgtc
    31441 ttatctttta atatttgtct ttgaggtaag tcgtatacta aaatttgaat acaaataatc
    31501 aaatcattga taaatttttt gtctacgatt aatggaggga cttgaatggt gttaattacc
    31561 tatcaaatca ttttattttt tattattagt ctaagttact atttaacttt aaatcattac
    31621 atggcagtca ctgtaggtaa cttcacttca atattcggca tgttcgcagc catactcttt
    31681 atgtactact acctactcta taaaagtccc gaatacaatc aacgcaaacg atttaaacat
    31741 ttcattcata tcactaattt gataataatt gcttttagca ccttcgtatt agttcattta
    31801 gcattaaaat tattcttcag catttaattt ccatctatga aaaaagcaaa gctcaaatct
    31861 gaactttgct ttaatttgtc acgcctttat cattttcaaa atagcctcta tgccagtttt
    31921 acaaacttgt agcaacaatt tttcatcaag caactgaatc acatcaaaaa cttcaattgt
    31981 ttacgttgca actaacttta tgttagaacg cacacaatat ctaatgttta aaatctatcg
    32041 tcgcaagatg cttcctgtaa ttatcaagtg ccattttcga ttgggttata caatctagaa
    32101 tcgcatgata atttaatgct acaaatcgat agtacaatat atctaccgtg aataactgtg
    32161 caaatagtga cgttgtagcc gccatacgca tttcattttc atcagttctg ccataaatca
    32221 atgcatagtc tgcaatttga gccactggat tattagctgt actagatata gttatgatgg
    32281 gaatactgta atgtgtggcc acctgtgcaa ttgactgcaa ttcactatga ctaccttgat
    32341 tcgtcacaaa aatcatgcaa tctctatcat catgcgtcgc aaatgttgac acaagtaaat
    32401 gcgtttcatg taataacctg acatttaagc caatacgaga taacttttga aaaagatcac
    32461 caatagtcaa actcgatgcg ccaaatccaa ataaaaatat tgtcctggca tttttcaaca
    32521 catcacaaat tgcatcaatt tgcgcatcca taatattagt agctacaaat cgcatcgtat
    32581 tcgttgctct agcaatcatt ttatttttca aagtttctac agattcattt tcaatcaatt
    32641 ctaaatgtgg attggttgca atatcttcgg gtaagtatcg agatatcgca atctttagct
    32701 cttgaaaacc ttgatgtgtc attttccgac taaatctaac aattgatgct gtactaacat
    32761 tcgtaacatc tgccaaatca ttcacagtca tatcaatgat tttatgtgga ttctttaaaa
    32821 tgtaatcagc gattatcttt tctgtcttcg taaaatcact caactgctta tcaatgcgat
    32881 ataaaatatt tgtcatcatt aatcacccaa caaatctgtc tgtcgcatcg cctttgtcgt
    32941 tccaaataaa tatgtacaaa cgaatccacc agcatacgca gcaagtaatc ctgcaatata
    33001 acctaaatac atattatctg agattaatgg taatagtgac acaccacttg ggcctattgc
    33061 tttggcacca atatgtccaa ttccacctat tacagcgcca ccaataccac caccaataca
    33121 agcagttaag aaaggtcgac ctaatggcaa agtcacacca tagattaatg gttctccgat
    33181 acctaggaaa ccaactggca atgcaccttt taaagtatta cgtaatgttg tgttgcgttt
    33241 acatcttacc caaagtgcta atgcggcacc tacttgtcca gcaccagcca tcgctgcaat
    33301 tggcaataag taagtagcac ctgattggtt aatcatttct atatgaattg gcgtaaaaat
    33361 atgatgaagc cctaacataa ctaacggtag gaagcttgca ccaatgataa atccactaaa
    33421 tacgccacca atactaataa ttccgttaac tactgaaact aaactgtctg aaacaaaacc
    33481 tgctaatggc ataaagataa agatagttaa tagtcctaca atcaacaatg caatagtcgg
    33541 cgttacaata atatcaatcg catttggcac aattttatgt aatctctttt cgacaatact
    33601 taaaatccaa acggcaaaaa taacgccaat aatcccacct tgtccaggtt gcaatggttc
    33661 tccagtgaag acattcatta aaatattttt accagcaata cccgttaata acgttgtacc
    33721 accaatcacg ccaccaagtc ctggtgtcgc accaaattct ttagccgcat taataccagt
    33781 gaaaatagct aagtatgcta acataccgtc tttaatgaca ttaaatactg ttataagttg
    33841 cgtaatccaa gcacctgaaa tatagcctgc caccattaag ttactcagta ctgctgcaat
    33901 accaccaatt aatccagctc caataaatgc aggaatcaac ggtataaaga tattggcaat
    33961 tgatttcaat actttattca acttaccatt cttttgtttt gctttatgcg cttccttatt
    34021 cgcctttgct ttatcagctg catatgattt atagtccatt ttttcactat cattgtgatg
    34081 gtgtggtatt gggtcaccta gtttaacacc acttaattcc gccatatgat tagccacttt
    34141 attgactgta ccaggtccaa ccacaacttg aatgcgttca tcgtgtataa cacccatgac
    34201 accatcaata tgccttagtt cttggtcatc tactttattc tcatctaata ctttaatacg
    34261 cacacgtgtc atacagttca tgacactatc tatattatcc ataccaccta ctgcagcaat
    34321 aattcgttct gcaagttgtt gttctttggt catttaaatc cctcctaagg ttgtctatct
    34381 ctgattgctc gtttaacaat gtcaccattg tttaataacc gtcttgttgc ttcttcctta
    34441 gaaatgccac acatacccat aactgtcgca actttcacat catgctcaga tacctgatat
    34501 aacgccattg cttcatcata tgtgatagca catatttctt gaataatacg cactgaacgg
    34561 tcgatcagtt tttgattggt tgctttaaca tcaatcatga ggttatcgta aacttttccg
    34621 acaccaacca ttgtgatggt tgaaatcata tttaaaatta acttttgtgc tgtaccagac
    34681 tttaaacgtg ttgaaccagt taatacttct ggaccaactt taacttctac tggatactgc
    34741 gcaatttcac ttataactgc atgttcattg catgaaatag atactgttgt agcaccgatt
    34801 gtgttagcaa atgttaaacc gcctataaca tatggcgttt tgccactcgc ggcaattcct
    34861 ataacgacat cttttgatgt taaatctata tttttcaaat cttcttccgc taattttttg
    34921 tgatcttccg caccttctac agccatcgtc atagcatgtt gtccaccagc aataatacct
    34981 ataatttcat gagggtcagt attgaatgta ggtacacact ccgctgcatc taagacaccc
    35041 aaccttccac ttgtacctgc accgatataa atcaatcgtc cacccttttt atactgtgca
    35101 attgtttttt taattacttt tgtcaattgt ggtattgcct ttcgaactgc taacgggact
    35161 tgctgatctt ctttattcat cgtaattaaa gcctcttcca cagtcatttc atcaagatgc
    35221 atcgtcgctt cattacgcgc ttcggtcgta ctattttcca tcacttctta cactccctag
    35281 ttttttgaaa atcaaatgta tcattcggct cgatacaact taacagtggt aagtcttctt
    35341 taataatttg tgcaacaaca ttcacattgt catgtgcact aagcgtttgt ctcacaattt
    35401 gcatttcgcc ttgataacgt ccgttattca aattatcaac ggttactgaa ccaatgcgtc
    35461 gttgcgtcgt aaactgtggt tgaatcgaat gtggacatat ttgtcttgac gtttccgaac
    35521 gaatgacatt ttccggatta tccgggcgta ctttatgaca catatcgaaa aggtaagtca
    35581 ctgtcgtatc aaacacttct tcaatacata acgtgaaatg cctatgcttg caaaaatcta
    35641 taagttgttt tgcctgcctc atttcaatca atgagtctcc aactaacact tcagatacac
    35701 cagtttcttg taataattta gctgcaacga caggatgact atgtctcgtt gcttcaattg
    35761 ttggcaagcc tttatgcaaa ggacctcgca aatcactccc tacaataaaa ccatatattt
    35821 gtgcctttgg attaaattga taaatgagtt catttttctt attgaccaag tcaacagata
    35881 atcccgtatc tggtcttgga taatagttat gacaaaatga aagtaatgta aaatcattca
    35941 attgttgatg taagcttgtt aacaattccc gggaaataat acttgcattc aaacagcact
    36001 ttaaaccctg tgccattatc gcttcgattg cctcaattga tgtactatga tcgatacgaa
    36061 tcataaattg tgcatcatat tgtcgaagat ggtcataaaa agatggtgtt aaaatagatg
    36121 gattagcatc tatgaggtaa gtcacttgtt catgttttaa taaattgagt agttttgtga
    36181 aataatgata ttttgtctcg tcatcttctt ctggtatttg tacagatgta aaaatcattt
    36241 ggtaaccttg tttaatcatt cgcttaatat acgcttcatc taaaggttgt cctaaataca
    36301 ctgaaaagcc tgtcaaagta gccctcctta acaatataat tattaggaaa atatagttga
    36361 gttgtgtaat cgcttacatt ttactataag agaaaacaca ttacaatatt aatcagttaa
    36421 agcctgttca ttgtaataat cttacatatt tctgtcacaa gttaattatt acaccatcaa
    36481 agattatcct ttcttttaag tgctgataat agctgctact gctggattat tacaataact
    36541 tttatacatt ttattcagga ttatcttata ttatgtttta ataataatct gtgaacaatt
    36601 aagagatttg aaattgaatt taataattgt attgaaaacg catacttcac catgctaaaa
    36661 taggagtcgc aaacaaataa gattcaataa gatgtgatgg taagctagat aaactaacgt
    36721 atcagtttta tatactactt agtcaattgc ttcttttaca ttaattacat acaccaacgt
    36781 gttactaagt aagattaggc atgagttaaa ttaagctgtg atggttacca acacagtcta
    36841 tttgctcgtg tcttttttta ttgaatctta aataataaat acaactttgg aggttggaca
    36901 agtgaggaag aaacttttcg gtcaattgca acgtattggt aaagcgctaa tgttacctgt
    36961 tgcgatttta ccagcagctg gtctgttatt agctatcggt acagctatgc aaggtgaatc
    37021 attacaacac tacttgccgt ttatacaaaa tggtggcgta caaactgtcg ctaaattaat
    37081 gacaggtgct ggtggtatca tttttgataa cttgcctatg attttcgcat taggtgtcgc
    37141 aatcggatta gctggcggtg atggcgtagc agctatcgca gcattcgtcg gttacataat
    37201 catgaacaaa acaatgggcg actttttaca agttacacct aagaatattg gtgatccagc
    37261 gagtggttac gctagcattt taggtatccc aacattacaa acaggtgtgt tcggcggtat
    37321 tataatcggg gccctggcag cttggtgtta taacaagttc tataacatta acttaccatc
    37381 ttatttaggt ttcttcgctg gtaagcgttt cgtacctatt atgatggcta caacatcatt
    37441 tattttagca ttcccaatgg cattaatttg gccaacgatt caatcaggat taaatgcatt
    37501 cagtacagga ttattagatt caaatactgg tgttgccgta ttcttatttg gtttcatcaa
    37561 gcgtttatta attccattcg gtctacatca cattttccac gcaccgttct ggttcgagtt
    37621 tggttcatgg aaaaatgcag ctggtgaaat tattcacggt gaccaacgta tctttatcga
    37681 acaaattcgt gaaggcgcac atttgacagc tggtaaattc atgcaaggtg aattccctgt
    37741 tatgatgttc ggtttacctg cagcagcttt agcaatttat cacacagcta aacctgaaaa
    37801 taagaaagta gtagcaggtt taatgggttc tgctgcttta acatcattct taactggtat
    37861 tacagaacca ttagaattct cattcttatt tgtagcacca ttattattct ttattcacgc
    37921 agtacttgat ggtttatcat tcttaacatt gtacttatta gatcttcatc taggttatac
    37981 attctcaggt ggtttcatcg actacttctt actcggtata ctacctaata agacacaatg
    38041 gtggttagtc attcctgtag gtcttgtata cgcagttatt tactacttcg tattccgatt
    38101 cttaattgta aaattaaaat acaaaacacc aggtcgtgaa gataaacaat cacaagcggc
    38161 tactgcttca gcaactgaat taccatatgc agtattagaa gctatgggtg gcaaagcaaa
    38221 cattaaacat ttagacgctt gtatcacacg tctacgtgtt gaagttaacg acaaatctaa
    38281 agttgatgtt cctggtttga aagatttagg cgcatctggt gtattagaag tcggcaataa
    38341 tatgcaagca atttttggtc ctaaatctga ccaaatcaaa catgaaatgc aacagattat
    38401 gaatggtcaa gtagtagaaa atcctactac tatggaagac gataaagacg aaactgttgt
    38461 tgttgcagaa gataaatctg caacaagcga attgagccat atcgtgcatg caccattaac
    38521 tggtgaagta acaccattat cagaagtgcc tgatcaagtg ttcagcgaaa aaatgatggg
    38581 tgacggtatc gctatcaaac cttcacaagg tgaagttcgt gcaccattca acggtaaagt
    38641 acaaatgatt ttcccaacaa aacatgcaat tggtcttgta tcagatagtg gtttagaact
    38701 attaatccac atcggtttag acactgttaa attaaacgga gaaggcttta ctttacatgt
    38761 tgaggaaggt caagaagtta aacaaggtga tttattaatc aactttgatt tagactacat
    38821 ccgcaatcat gcaaagagtg atattacgcc tattatcgtg acacaaggaa acattacaaa
    38881 ccttgatttt aaacaaggtg aacatggcaa catttcattt ggcgatcaat tatttgaagc
    38941 taaataatgc ttactataaa caggtgcgta taccttcata aggtgacgcg cctgtttttt
    39001 ctttgctatt gtattttgca gcatcattga tagttcgctc tccccttaaa ttttgaattt
    39061 taagatcatc aattaaagcc ccccttcata ctcatttcct aaaaaatatt aattgttcac
    39121 tattgttagc gttttcacaa caaagtcaac ttccttgacc ttacactata ttcgaggcta
    39181 tcattttaag tgtaaatata gagaaaaggt ggcttttttt atgaaacaac gcattggagc
    39241 ttacttaatt gacgctattc atcgagcagg cgtcgataaa atttttggtg ttcctggtga
    39301 ttttaatctc gcttttctag acgatattat cagcaatccc aatgtagatt gggtaggaaa
    39361 tacaaatgaa ttaaacgcaa gttacgcagc ggacggttat gcccgtctta atggactcgc
    39421 tgcattagtt actacatttg gtgttggcga attaagtgcc gtcaacggta tcgcaggttc
    39481 atatgctgaa cgcatacctg tcattgcgat tacaggtgcg ccgacacgtg ctgttgaaca
    39541 aggcggtaaa tatgtacatc actcacttgg tgaaggtaca tttgacgact atcgaaaaat
    39601 gtttgcacat ataaccgttg cacaaggtta tatcacacct gaaaatgcaa caaccgaaat
    39661 accacgttta attaatacag caatcgccga aagacgccca gttcatttac atttaccaat
    39721 cgatgtcgca atctctgaaa ttgagatacc gacaccattt gaagtgacgg caactaaata
    39781 tacggatgca tcaacatata tagagttatt agcaactaaa ctgcatcaag cgaagcagcc
    39841 tatcatcatt actggacatg aaattaacag ttttcacctc catcaagaat tagaagattt
    39901 tgtaaatcaa acacagatac cagtagcaca actttcatta ggaaaaggtg cttttaatga
    39961 ggaaaatcca tattatatgg gtatttacga tgggaaaatt gccgaagata aaatacgaga
    40021 ttatgtggac aacagcgatt taattttaaa tattggagcc aaattaacag attcagcaac
    40081 agcaggtttt tcataccaat tcaatatcga tgatgtcgtt atgttaaatc atcacaatat
    40141 caaaattgac gatgttacaa atgatgaaat atctctacca tcattgttaa aacagttatc
    40201 caatatttca tatacgaata acgcaacgtt ccctgcgtat catcgtccaa catcacccga
    40261 ttatactgtt ggcacagaac cattaacaca acaaacttat tttaaaatga tgcaaaattt
    40321 cttaaaacca aatgatgtca tcattgctga tcaaggtaca tcattctttg gtgcttatga
    40381 tttagcatta tacaaaaaca atacttttat agggcaaccg ttatggggtt ctatcggcta
    40441 tacattacct gcaacattag gttcacaatt agcagacaaa gatcgtcgta acttattatt
    40501 aattggtgat ggctcattgc aactaactgt tcaagctatt tcaactatga ttagacagca
    40561 tattaaaccg gtattatttg tgattaataa tgacggctat acggtagaac gacttattca
    40621 cggcatgtat gaaccttata atgaaattca catgtgggat tataaagctt taccagctgt
    40681 atttggtggt aaaaatgttg aaattcatga cgttgaatca tcaaaagatt tacaagacac
    40741 gtttaatgca attaatggtc atcccgatgt gatgcatttt gtcgaagtca aaatggctgt
    40801 cgaagacgca ccgaagaaac tcatcgatat cgctaaagct ttttcacaac aaaataaata
    40861 atttcatcgt atacagggta taagtttaag cgaatacttt attaaacgaa taggactctg
    40921 atataagatg attaatttta ataaaaccgc tttagtgtta atcgacctgc aagaaggtat
    40981 tcttaaaatg gattatgccc catatacagc tgaaaatgtc gttcaaaacg ctaataaatt
    41041 aatagatgtt tttagaaaaa acaatggctt tatcgctttt gttcgcgtga atttctatga
    41101 tggtaaagat gtattgcaac caaatgcaat gatctcatta ccaccaaaag aaggcgacga
    41161 ctacagtcgt ttccatcatt tattagacaa gagagatgac gattttgtca tagacaaacg
    41221 acattttagt gcatttgtag gaacagattt ggacttacaa ttgcgacgtc gaggaattga
    41281 tacgattgtt cttggtggtg tcgcaacgca tattggcgta gatacgacag cgcgagatgc
    41341 ctatcaatta aactacaatc agttttttgt tacagatatg atgagtgcac aaaacgaaac
    41401 gctacatcaa tttccaatag ataatgtatt cccattgatg ggacaaacaa taactacaaa
    41461 cgactttcta aatatattga actaaacata tacttccccc cttcgatcat gttgaggggg
    41521 atctttattt cacaaagtat taatacgtcg ggttgtctaa ccttctatat ttaacatatt
    41581 ctatatctgt taaatcgttc ttaacttacg cccctactac ataaaaaaca gtatttattc
    41641 cggaattttc aaaaaattta gtatttattg caaaattatg tatcacttta tgtttaattt
    41701 ttgatattat cttaattaag tagattttta taagttctaa aaaggagaac aaat
//
LOCUS       DAMQYT010000007        40833 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMQYT010000007 DAMQYT010000000
VERSION     DAMQYT010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 40833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40833
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.007"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(408..506)
                     /locus_tag="PGA33_000268"
     CDS             complement(408..506)
                     /locus_tag="PGA33_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472109.1"
                     /translation="MNWIIELDLWLWCVGAINLINAVNDYANEILL"
     gene            complement(711..1307)
                     /gene="recR"
                     /locus_tag="PGA33_000269"
     CDS             complement(711..1307)
                     /gene="recR"
                     /locus_tag="PGA33_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDG6472110.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            complement(1314..1631)
                     /locus_tag="PGA33_000270"
     CDS             complement(1314..1631)
                     /locus_tag="PGA33_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDG6472111.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            complement(1721..3418)
                     /gene="dnaX"
                     /locus_tag="PGA33_000271"
     CDS             complement(1721..3418)
                     /gene="dnaX"
                     /locus_tag="PGA33_000271"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDG6472112.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYPALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPVQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            complement(3487..4011)
                     /locus_tag="PGA33_000272"
     CDS             complement(3487..4011)
                     /locus_tag="PGA33_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDG6472113.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            complement(4149..4250)
                     /locus_tag="PGA33_000273"
     CDS             complement(4149..4250)
                     /locus_tag="PGA33_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472114.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSELDVQFE"
     gene            complement(4319..4588)
                     /gene="ffs"
                     /locus_tag="PGA33_000274"
     ncRNA           complement(4319..4588)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PGA33_000274"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4650..5378)
                     /gene="treR"
                     /locus_tag="PGA33_000275"
     CDS             complement(4650..5378)
                     /gene="treR"
                     /locus_tag="PGA33_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDG6472115.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
     gene            complement(5403..7043)
                     /gene="treC"
                     /locus_tag="PGA33_000276"
     CDS             complement(5403..7043)
                     /gene="treC"
                     /locus_tag="PGA33_000276"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDG6472116.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWYSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTIGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGCVVIQTGTVENNTISGFGAIVIETNA"
     gene            complement(7107..8534)
                     /gene="treP"
                     /locus_tag="PGA33_000277"
     CDS             complement(7107..8534)
                     /gene="treP"
                     /locus_tag="PGA33_000277"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDG6472117.1"
                     /translation="MAVKREDVKAIVTAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
     gene            complement(9005..9097)
                     /locus_tag="PGA33_000278"
     tRNA            complement(9005..9097)
                     /locus_tag="PGA33_000278"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9059..9061),aa:Ser,seq:tga)
     gene            complement(9366..10829)
                     /locus_tag="PGA33_000279"
     CDS             complement(9366..10829)
                     /locus_tag="PGA33_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HDG6472118.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLAAAEELNLLGYQVTIYERARESGGLLMCGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            complement(10847..15346)
                     /gene="gltB"
                     /locus_tag="PGA33_000280"
     CDS             complement(10847..15346)
                     /gene="gltB"
                     /locus_tag="PGA33_000280"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDG6472119.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEVVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATSGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            15527..16411
                     /locus_tag="PGA33_000281"
     CDS             15527..16411
                     /locus_tag="PGA33_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDG6472120.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPSLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            16526..17644
                     /locus_tag="PGA33_000282"
     CDS             16526..17644
                     /locus_tag="PGA33_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDG6472121.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMADGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            17641..18423
                     /locus_tag="PGA33_000283"
     CDS             17641..18423
                     /locus_tag="PGA33_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDG6472122.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(18592..19077)
                     /locus_tag="PGA33_000284"
     CDS             complement(18592..19077)
                     /locus_tag="PGA33_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6472123.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
     gene            complement(19067..19462)
                     /locus_tag="PGA33_000285"
     CDS             complement(19067..19462)
                     /locus_tag="PGA33_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDG6472124.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVHALL
                     REVKEELNLTLTMDEIEYVGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            19611..19880
                     /locus_tag="PGA33_000286"
     CDS             19611..19880
                     /locus_tag="PGA33_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472125.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            complement(20065..21069)
                     /gene="aaa"
                     /locus_tag="PGA33_000287"
     CDS             complement(20065..21069)
                     /gene="aaa"
                     /locus_tag="PGA33_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HDG6472126.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRG
                     YNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(21401..22243)
                     /gene="gmpC"
                     /locus_tag="PGA33_000288"
     CDS             complement(21401..22243)
                     /gene="gmpC"
                     /locus_tag="PGA33_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HDG6472127.1"
                     /translation="MKRLIGLVIVALVLLAACGGNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            complement(22280..22939)
                     /locus_tag="PGA33_000289"
     CDS             complement(22280..22939)
                     /locus_tag="PGA33_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6472128.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            complement(22943..23968)
                     /locus_tag="PGA33_000290"
     CDS             complement(22943..23968)
                     /locus_tag="PGA33_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDG6472129.1"
                     /translation="MIEFRQVSKTFNKKKQKIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGK
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFEDTAINQYFKEKN
                     IQFEEVH"
     gene            complement(24262..25404)
                     /locus_tag="PGA33_000291"
     CDS             complement(24262..25404)
                     /locus_tag="PGA33_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HDG6472130.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIATLENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            complement(25388..26302)
                     /locus_tag="PGA33_000292"
     CDS             complement(25388..26302)
                     /locus_tag="PGA33_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDG6472131.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGAEVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENNNEQEN"
     gene            complement(26519..27856)
                     /locus_tag="PGA33_000293"
     CDS             complement(26519..27856)
                     /locus_tag="PGA33_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDG6472132.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIVIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            complement(28021..28134)
                     /locus_tag="PGA33_000294"
     CDS             complement(28021..28134)
                     /locus_tag="PGA33_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472133.1"
                     /translation="MGIAPEVVMWRDVGDIDGVVCGEILAHVYSVEPENLM"
     gene            complement(28312..29046)
                     /locus_tag="PGA33_000295"
     CDS             complement(28312..29046)
                     /locus_tag="PGA33_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HDG6472134.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            complement(29083..29757)
                     /locus_tag="PGA33_000296"
     CDS             complement(29083..29757)
                     /locus_tag="PGA33_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDG6472135.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISLA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            29795..30211
                     /locus_tag="PGA33_000297"
     CDS             29795..30211
                     /locus_tag="PGA33_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472136.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVRRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            complement(30440..30802)
                     /locus_tag="PGA33_000298"
     CDS             complement(30440..30802)
                     /locus_tag="PGA33_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HDG6472137.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(30964..33669)
                     /locus_tag="PGA33_000299"
     CDS             complement(30964..33669)
                     /locus_tag="PGA33_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HDG6472138.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPEMTIESVLTHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHYFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVAKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQP
                     HSVLLNDTQAVDENNSELNQMGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAVAEHHTSTDTLAWVYVPDT
                     LSSIALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGTLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVIQAPDYVVARLLANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            complement(33682..35166)
                     /locus_tag="PGA33_000300"
     CDS             complement(33682..35166)
                     /locus_tag="PGA33_000300"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HDG6472139.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGITLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILGWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYVITHNYFYAVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKAFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            35687..35752
                     /locus_tag="PGA33_000301"
     CDS             35687..35752
                     /locus_tag="PGA33_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HDG6472140.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            35784..35849
                     /locus_tag="PGA33_000302"
     CDS             35784..35849
                     /locus_tag="PGA33_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HDG6472141.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            35901..35969
                     /locus_tag="PGA33_000303"
     CDS             35901..35969
                     /locus_tag="PGA33_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HDG6472142.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            36033..36095
                     /locus_tag="PGA33_000304"
     CDS             36033..36095
                     /locus_tag="PGA33_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HDG6472143.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(36754..37956)
                     /locus_tag="PGA33_000305"
     CDS             complement(36754..37956)
                     /locus_tag="PGA33_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDG6472144.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKMLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTELDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            38075..38335
                     /locus_tag="PGA33_000306"
     CDS             38075..38335
                     /locus_tag="PGA33_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472145.1"
                     /translation="MSALLYNGDKDISHERESRCINMLFVILVLYVTGIAFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            complement(38625..38939)
                     /locus_tag="PGA33_000307"
     CDS             complement(38625..38939)
                     /locus_tag="PGA33_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472146.1"
                     /translation="MTLIEPDMTLRMPDISTTVETLNLISKMEAQKENIRTVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(38958..39281)
                     /locus_tag="PGA33_000308"
     CDS             complement(38958..39281)
                     /locus_tag="PGA33_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472147.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            complement(39278..40606)
                     /locus_tag="PGA33_000309"
     CDS             complement(39278..40606)
                     /locus_tag="PGA33_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472148.1"
                     /translation="MLSRKYKIDLKAINQNTESTSAISKASYEVENANNNGLSKRDVI
                     NQFNDLKKMKKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKK
                     LKEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFI
                     TGHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITR
                     FVAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYA
                     EENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQ
                     LIDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMV
                     REPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            complement(40596..>40833)
                     /gene="lpl10"
                     /locus_tag="PGA33_000310"
     CDS             complement(40596..>40833)
                     /gene="lpl10"
                     /locus_tag="PGA33_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl10"
                     /protein_id="HDG6472149.1"
                     /translation="YEAQYKMNNSDKNVKKLREIYPITTNNSPNLKLYIDGDIKGSSV
                     GYKKIEYKFSKDKGQETTLRDYLNFGPSEGENVE"
BASE COUNT        12793 a         8389 c         5912 g        13739 t
ORIGIN      
        1 aacatttaag tcaataactt tttaaaatta attttcgtta gtaattcaag ttatttctta
       61 agttgagttg ttttgtttgc ctcaacaaat aaatactata caggctttca aactgcttaa
      121 caacagtaaa aattacacct ttgtcatctg tactgaacgc ccttatccta aaaatcgaac
      181 ttaaaaaatc cccacttata aatagtacgt tttttagaac aagaaacaga tttttctcta
      241 accatatcaa tcaaaaaaaa cgcaaaacag aacgttattt ttttaccaga aattaaaaat
      301 aaatttacaa gttttaaata atctctcaaa acaccgctat acaggctgat ttaataagct
      361 ttagaccaaa gaagtacaac ttactaagcc agcatctatc atttcaatta caaaagaatt
      421 tcatttgcat agtcattaac cgcatttatc aaattaatag caccaacaca ccataaccat
      481 agatctaatt cgataatcca attcacatca ttactattag aagtaacaaa tcacaaccaa
      541 cggcttgctt cctaaagatt acccatccct cagtaaaact aggtaaacca taccaccgcc
      601 acaccgcaaa taaagtagta catcacacca caccataaaa aaagccacaa tgattacact
      661 gtgacttttc ttactatagt attaaaatca aaaatgttta atagaagaca ttacatttct
      721 gttctacctg cgattgcttt agataatgtt acttcgtcag catactctaa atcgccacct
      781 accgataacc cttgtgctaa tctcgtcact ttgataccta taggcttaac taatctagaa
      841 atatacatgg ctgtagattc cccctctaag ttcgggttca tagctaagat taattcgcta
      901 acttcatcgt ttttcaagcg ttcaatcaat gaaggaatat taatatcttc tggtccaatg
      961 ccatccatag gcgaaataga cccatgtaaa acgtgatata aacctttgta ttctctcatt
     1021 ttttccatag ctatgacatc tttgtcatct tccacaacac aaataactga acgatctctt
     1081 tgcttatctt cacaaatata acatggatca ttttcagtaa tgtgaccaca tacgctacaa
     1141 tatgttaatt ctctcttaac atctactaat gctttggcaa actgaacaac atcgtcttct
     1201 ttcatatcta aggtatgaaa agccagacgt tgggctgtct ttggaccaat gcctggcaat
     1261 ttcataaagc tatcaataag ttttgatata ggttctggat aatgcatcta tgatcacatt
     1321 ccagggatgt ttaagccttg agtatgttta cctaaacgtt cttgagtaag ctcatcagct
     1381 ttattcatcg cttcattagt agctgctaac actaagtctt gtagcatttc aatatcgtct
     1441 gggtctacag cttcttcttt gatttcaacg tcgacaactt ctttatgacc agttacagta
     1501 actgcaacca tgccaccgcc agctgttcct acaatacgct cttctttaag tttttcttgt
     1561 tcttgagcca ttttcttttg cattttttgc atttgtttca tcatttgttg catgtttccg
     1621 ccaccgcgca tattccattt cctccttgaa aatcaataaa tatttatcaa taaaatgatg
     1681 tttctttcat tatacatacg attatatcgc ttgtcatgta tcactcttca tctatcacat
     1741 gtacagtttc ttcaccgaaa agatcttttg ctttttgagc aatatctgtt tgttgtgctt
     1801 gttgctttgg catatcatcg ccttcgtttt tacgattttg taaatattcc gttcgaactc
     1861 tttgccattg atctgatggt acaccaacaa ctttaacgtt tttattaacg atattacata
     1921 caacactttc tatactacta cgtttctcgt cgtctttatt gacgatttca caatggatct
     1981 cttcctcaaa tttcacaagt acgtgatctt cacttgccgc cacaggttcc gaattttgca
     2041 ataaactaac gagtgatttt ttatcattat ttttggcatg atcaatcact tcttgccaat
     2101 gatctttcaa caatttgata tctgccttat tcgctttatc tagcactttt gcaatttgtt
     2161 gcattgaaaa tgcattttta gatttttgta tgcctctcgc aggctttttc gaagattttt
     2221 gaacaggagc gacactcact ccttgtgctt ttagtgtttt tagttcttgc tctaactgtt
     2281 ccatacgttg caacaataca tctgtgtttg gcgatgaagc aatttgtgct ggttcagcta
     2341 cattcgcaat cacttgtggt tgacccttaa tctgctcagc taattttact aacaacactt
     2401 caaaatgaac gttttgattc acactaaaac gaatcgacac taatgtatca ttaataagat
     2461 caatcatttg atataacata tctaattcta agttcatcag tgctggatac tcagtatctt
     2521 tctcagatgt tttattcata atcgtatctc tgacaaaata aatcatatca tttattaggc
     2581 gattcacttc tttaccttct gttataaact gatggtattt tttaaaagat gcttgtacgt
     2641 caccttgtac aatatcatca aacaagtgat ccaacgcttc atcatgtacg ctacctgtga
     2701 cattcaacgc atcttgcaat gttaacgtac catcaccaaa tgcaatagcc tgatccataa
     2761 tacttaatgc atcacgcata cccccttcag acgctttagc gataaatgcc aaggcttcat
     2821 cttcacattc aatttgttgt gcatctgcta caaattttaa acgttcaaca atttgatcta
     2881 ggctaattgc tttaaaatca aaacgttgtg ccctagaaat gattgttgga gggattttat
     2941 gtggttctgt cgttgccaat ataaaaatag cgtgtgctgg aggttcttct aacgtcttta
     3001 aaagggcatt aaaagcacct gttgttagca tgtgcacctc atctataata taaactttat
     3061 atttcgattc acttggtgca tatttaactt tgtctctaat atttcttatt tcatcaaccc
     3121 cattattact agcagcatca atttctatca catctgaatt agtcccctgc gtaatgcctt
     3181 tacaaatatg acattcatta caaggttctc catcagtgct atttagacag ttgattgctt
     3241 tagcaaacac tttggcaata ctcgttttcc ccgtacctct cggaccacta aaaatataag
     3301 catgcgactg tttttctttc gaaatcgcat tgcgcaatgt cttcgtgaca tgttcttgtc
     3361 cgacgacatc ctcgaaactt tggggtctgt acatacgata taaggcttga taattcaagt
     3421 tagcacctcc ataaacaatt acctctcatt atagcatgat aataccttta cttcttaatt
     3481 gaacaattaa taaaaatgtt ctggaaattt tacgattccg tgtggtgcat ccgtcaaatt
     3541 atcccataaa tattttacaa gtaacggtgc atcaccagat tctaatttaa tgtcatgctc
     3601 agcagcattt tgataaccca acttttcaaa gtagtcaaaa caatggtcta caacaaccgt
     3661 actatactct tgtgctttgg cacgctcttc tactgcttga accaagccac gacctaattt
     3721 ttgtccacgt aattcaggat gaactgataa agaggcaatc gccaaaccat aatacgtctt
     3781 atcatcacta ttaatttcta cttcaattaa taaaacgtgt ccaacgacat cgttattttc
     3841 atttttcgct attacttcta attcaaaatt atagcaagga gattttctta aatgttttac
     3901 tttcgcacgt gcttgccaac tcgtttcagg attatcatca aaactttctt caatactatt
     3961 taaagattta tcataatcta actctgttaa agtacttaaa tatatttgca tatgtcctcc
     4021 gtaggcattt gattgtcaat aatcataacg tatctcatta ataattctat tgtaagatac
     4081 tccccctcat ttcaccatca tttctttatc atcaaattat aacttcttac ttttcattga
     4141 cacaaaaatc attcaaactg cacatcgagt tcacttgaat caaacttcac atataaaaaa
     4201 gctacttccc acaaacatgt tccacgtata atacgctgaa ttgtcttcaa gaaagtagct
     4261 tctataatta tatattttca actcttaata atcggtttaa tatttaaaaa ataaaaaacc
     4321 gtgcacctaa gcatcgaacg taattcacga acgtaaccaa agtcgttagc taaatctcgg
     4381 ctaccctacg gcacatatga tgatccactt aatgctgctt ccgtcaggac ctgacatggt
     4441 tcatgggttc atattgcata ggaccgaaat cttcaaacac tacgtgcttt gggcagactt
     4501 cgcaaaaata cggcctcaac aaaggaatta agcctcgcat aaagcggatt tcgagtacag
     4561 ggaaccgcta cctccccacc tagcacggca agatatatat tactatattt taatagttaa
     4621 ttgcaagtat aaatcattta tatcattgtt tactttatac gacgtcttga gaagtcatta
     4681 aatttaaatt catttgcaag atgttttgaa atattatatt gaaacggcat tgtattttct
     4741 aaatacacaa tacttcgaac tgttgctgaa taagccaccg atacatcacc aaacaattga
     4801 tatgcttgtt catcaaacgg ttcaaaagta atagacttac ttgaataact aatattaaga
     4861 tttaatacct tttccaaata atcataaata gaatcactcg caacatcatt acctatatct
     4921 gaaacaatcg actttagaaa ataatcttca tcaacaatct tcacatgttg gttaagccga
     4981 cgagttctaa caatatgaat gagtgattca ctagaattga tgtttaaagc atgttgaact
     5041 tctggaactt catgcgcttc aacaacctca ttcacaacaa cttcagttaa atatgcgacg
     5101 cccatttctt cttgcatttc tttaaaacta acaagttcag aaaatggaaa ctctgtaacc
     5161 tcctgataaa tgacaagtga ccctttacca tgaatctttt gaatcatgcc gtctaatgcc
     5221 aacaaatcta atgccttacg cacggtctct cgagatgact ggtacaattg caccaaatca
     5281 tgttcagacg gaatttgttc accatattga atctgcccgt ttaatatatc ttctttcaac
     5341 gcctcataaa tcttcataaa ctttttttgt ttcgccatat aaacgcatcc actcaattta
     5401 ttttacgcgt ttgtttcgat tacaattgca ccaaacccgc ttatcgtatt attttccact
     5461 gtgcctgttt gaatcacaac acaaccttct ctagccaatc cttctggcaa atcaacagcc
     5521 gatgctgaga aattcgcaat tactagccat tgttgattct tataatgacg ttcataaaca
     5581 aataaatgat catgatccat aaaacgtggc acaatgtctc catacgtaat aatatcatgc
     5641 gtatgtctta attgtattaa tttttgatac gtatagaaaa tagactcttt attctgaagt
     5701 gcttgtctaa cgttgacctg atgataattt tccgaaatat caatccaagg cttaccagct
     5761 gtaaatccag cattaacatc atcactccat tgcataggcg ttctcgaatt gtctcgtgac
     5821 ttctgtccta aaatcgctaa cacatccgct tcagcatgcc cttcacttaa caactgatgg
     5881 taggcattaa tcgattcaac atcacgatat tgtgctattg atgtaaaatg tgggtccgtc
     5941 ataccaattt cttcaccttg gtaaatatat ggcgtccctt gcaacatatg cagtgcgata
     6001 gctaacattt tagcactttg tatcctcatc tcttccgacg tatcatcacc aaatctagac
     6061 actacccgtg gctgatcatg attacaccag aaaatcgcgt tccatccgcc accgtcataa
     6121 atacctcgtt gccattgcat cagaatttcc tttaacttat gaaaatcaag cttcgcattt
     6181 gtccactttt caccatcaac ataatcaacc tttagatgat gaaaattaaa aacactattc
     6241 aattcttggc gttctggttg tgtatactta atacaatttt caatcgtcgt cgaagacatt
     6301 tctcctatag tcatcatgtc agtgttacca aacgtttgac gatttaattc atgcagaaac
     6361 tcatgcactc taggaccatc cgtataaaat tctttaccta ttttgtcaga gtccttaaat
     6421 tcacctttag aaattaagtt aatgacatca aatcgaaaac catcaacgcc gaagtctatc
     6481 caatgattga ctatgcgata taacgattga cgtacttccg gattatccca atttaaatca
     6541 gcttgactga catcaaataa atgtaaataa tattcatctg tctcagaatc atacttccat
     6601 gcattaccac cgaatttaga ataccaattt gttggcggcc cgtcttcaga tgatctgaaa
     6661 aagtaataat ctctataagg gttatcttta gatttacggg cttctttaaa ccattcatgc
     6721 tccgtcgacg tatgattaat gacaatatct aacatcactt tcaagtcttt ttgatgcgca
     6781 actttgatta acttttcaaa atcatccatc gttccaaagt cttcattgat ttctaaataa
     6841 ttgctgatat catagccatt atcattcatc ggtgattcat acactggtgt taaccaaata
     6901 taatcaacac ccaataactt gatataatcc aatttttcta taattccatt gatatctcct
     6961 ataccattcc ccgtcgtatc attaaacgac ttaggataaa tttgatatac aacggatttt
     7021 ctccaatcta tttctttcga cacacaatta ccaccttata acgtaataaa ggacgtccaa
     7081 ataacgaacg cccctttttt attttattaa tcttcaacaa tttctttcgc tttttgttta
     7141 ctaaaatgag acatcacaat tgttagtata catggtacaa caatagcaat agctgtcaca
     7201 ataagatata ctggccaaaa ttctttttga attgaaatga atgctggcac gccaccaaca
     7261 ccaacttttc caagtacgtt attcatacca acgattgccc ccaatacaca agacgttgat
     7321 atcgcagcga taaatggata tttcagaggt aagttcacac cgaacatggc tggttcagta
     7381 acacctaaca taccagaaat acaagatgtt aatgccaagc cttcttcttt aaccatttta
     7441 cgacgtttat agacaaacca tgctccaaat gctgcagagc cctgacaaat attggaaatc
     7501 gcaacaattg gccataaata cgtaccgcct aagctgctac ccatcaattg gaaatctact
     7561 gctaaaaaca tatggtgtag tcctgtaatt acaagtggtg catataacaa tccatatatt
     7621 gctccgccaa gccatcctgc atgttggaat ataaatgtaa caccagatgt aatacctgta
     7681 ccaatcaata acgcaactgg tccaatgata ataaatgcta aaaatccagt aactaaaagc
     7741 gctacgggtc caacgaccaa catttttatc gaatcgtgaa cgactttatt taatcctttt
     7801 tcaattttag ctagaacgta agctgcaatt aaaactggca acacttgacc ttggtaattc
     7861 aactgcttaa tctctaagcc aaataagttc cacgtcggaa tattcccttt tgccaaatca
     7921 tactgagata ctaattgcgg atgcattaaa atcaaaccta agactatgcc tagaatcgga
     7981 ctaccaccaa atacacgcat actactccaa ccaattaatg ctggtaagaa aataaatgcc
     8041 gtactcgcaa tcacattaat gatgtttgaa atatcagcaa tttgtggata catctcaata
     8101 agtgcttttg gaccaaataa acctttcatt gtaagtaaat tattgattcc cattaacaaa
     8161 ccagctgtca caatcgcagg taatattggt ataaaaatat cccccaacaa tttgatcaaa
     8221 cgttgtactg gattcccttt ttgtgcagct gcttgtttcg cttcatcttt cgaagcttct
     8281 tgagcacctg tttcatcaat aaactgctta tacacttcat cgactgttcc tggaccaatg
     8341 acaatttgat attgatggtc tgctttaaac tgccccttga ccaacgcgtt attacttaat
     8401 gcgtctttat caactttact ttcatccttc agcactaaac gtaatcgtgt tacacaatgc
     8461 gttgcagctt caagattttc ttttccccca atagcggtta cgatggcttt tacatcttct
     8521 ctttttacag ccatatattt cactccctta atgtcttaaa acgagtataa cttgtctaga
     8581 caagttatgt aaaggctttc aacaaaatat ttttattttg tcacaattat ctggggtagc
     8641 catttcagaa aatcaatgag ccataacgac atatgtagca ataactcctc gctacaattg
     8701 cgttattaac aatacctcac cacgctctca ccattataaa gtgtagatgc atggccaact
     8761 cctaaattga aaaatgcata tctataaata gcaaacttgt ccacacaagc catccatcat
     8821 atttcacaaa acaaaaaaga catttagcta tgtatattgc acaagccaaa tgcccttcca
     8881 atctcaatgc cttatcattt ctattatcaa aaattacgag ccattcaaag acaaataaaa
     8941 aaggtaaata ctcaaaattt gagcacttac cttcattata tatagaattt aatataaata
     9001 ttgatggcgg aggaagaggg attcgaaccc ccgcgagccg ttaagcccct gtcggttttc
     9061 aagaccgatc ccttcagccg gacttgggta ttcctcctaa acacaatatt tatcatatat
     9121 gaacgttctg taaaagtcaa tattgttttt aagcattaca tgattaccac aaatcattgt
     9181 cttccactac agttgaccta taaacgaatg taaaaaatca ctacttataa ttaatctatt
     9241 tgttgtctta caaacctttg tttttaggtt acaaagatgg catgtgacgt tgatagatac
     9301 tagatttttt caaactcgat tcagcatgtc acaacgtcaa cgtaaccacc atttccatac
     9361 aaagattata cacaaacttt actagctaaa tactgatcta ctgctttcgc tacgcctcta
     9421 ccttctttaa ttgcccatac aactaaactt tgaccacgtc tagcatctcc agcagcaaat
     9481 accttttcat tattagtttg atagtttgta tcatccgcca cgattcgatt tctatccgtt
     9541 ttaatgttaa aagcattcgg tactgttggt tctgtacctt cgaagccgat tgataataat
     9601 acaaggtcag caggccaaaa tctttcaggt ccttctttca tgaccatacc attttcgcct
     9661 tgctctaaaa tttgagtata caaaccacgt atgtgtcctt tatcgtcaac atcgtaacgc
     9721 attgtttgaa caccatatgc acgtggttcc ttaccaaact tagcttcgta ctcttggtgc
     9781 gcatagtcca ttttaaacac cggcattgct aaaggccatg atgcattttc tgtaaatgta
     9841 attgcttctg gcaatttcgt atatttatta aattgaacaa tcgatttaca attttctctt
     9901 aatgctgtcg ctacacagtc tgcccctgta tcaccagcac caatgataat gacattctta
     9961 tcttttgcag ttattgttat atcatcaatt tctccattta acaactgcgt ttgttcagtt
    10021 aaataatcca tagcgaaatg tataccatca cccatgcgtc cttctaaagg taaatctcta
    10081 cctttttgtg caccagtaca taatataatg gcatcatact cagattctaa cgttgcttta
    10141 tcaatatcaa cgccgacttc aacaccatta atgaaagtaa tgcccgcttc ttccattaac
    10201 ttaatacgac gtcgaaccac atctttatca agtttcatat tcggaatacc acacattaat
    10261 aaaccgcctg attctctagc acgttcataa atagttactt gatatcctag tagattaagt
    10321 tcttcagcag cagctaatcc tgctggaccg cttccaacga ttgccacttt ttcatctcta
    10381 cggcgactcg gaactttcgg cgctacccaa ccattttcaa aagcttcatc aataattgtg
    10441 cgttcaatac ctttaatcgc aatcgattct ctattaatct tcatcacaca agcactttcg
    10501 catggtgcag gacatacacg ccctgtaaag tcaggaaagt tatttgtttc gcttaagcgt
    10561 tcataagcag ttttaaaatc ttgatgatac actaagtcgt tccattcagg aatgtagttt
    10621 ccaattggac aacctattgt ttccctacca tactgttgtc cggtttgaca aaacggcgtt
    10681 ccacaatcca tacatcgtgc accttggata gaggcatctt ctttagtaaa tcgttgttga
    10741 tatgctttat gatgcttcaa acggtctacc agtgataatt cacctaagta ctgtttgtca
    10801 tacttcataa atcctttaaa ttcacccatc gtatctcccc ctttccttaa tacacaacgg
    10861 ctggtttatg tttagcatcg attgttttac tgtcatcgta aaatgcagct aacatcgctt
    10921 catcttcatt gtcatgtaat gatttgtgca aatgaatttt ttgcatcatt aattgataat
    10981 ctttaggaat aactttaacg acgacatctt caatgcgatc aaaatgtttt aacacatgaa
    11041 tcgctctcgt actattcgtg tgtgacacat gttcttccag catttgctta atgaatgctt
    11101 tttcttcttg gtgtttaatc tttgtaaacg aaagcgtatc tagttgatta ttttcaacaa
    11161 aagcttctac atcagacggg ataacgtaag caataccacc actcatacct tgaccgaagt
    11221 tcttacctac atcacctaaa ttaatgacat gtccaccagt catatactct aatccatggt
    11281 cgccgatacc ttcaacgaca acatctacac cactatttct aatacagaat ctttctcctg
    11341 cactaccgtt aataaatgcc ttaccacttg tcgcaccata gaatgagacg ttaccagcaa
    11401 taatttcatt ttgtcgttct tcaaaaggtg ctttgacaat gaccgtacca ccagataatc
    11461 ctttaccaac atagtcattc gcatctccag tatgatgaat cattaagcct ttcggtgcat
    11521 atgctgcaag actttgacca gcatgaccat tcgtataaac attaattgta ttttcaggaa
    11581 gtcctgcttc tccatattgt ttcgaaatct cactacctgt aataacccct acatcacgtt
    11641 gttcattatt tactgtaaag ctacctgtat agcgacgccc ttcagcaata tatggcttcg
    11701 ttacttcata taaatttgtt aaatcaaatc catgctcaag attatgattt tgttgaattt
    11761 cttttgtgtt tggcccatcg aaaggacata acagtttttc aacatcaata ctagccgctt
    11821 tgctattcgc ttttaatgtt gatgatcgtt gtaataaatc agttcttcca actaagtctt
    11881 ctacacgttt caaacctaaa gatgctaaaa tttctcttaa ttcttgtgca ataaaatgca
    11941 taaaattaac aacatgatgt gctttacctc tatataaagc acgtaaatct ttgttttgag
    12001 ttgcaactcc tactggacat gtatctttat ggcatacacg catcataata cagcccaaca
    12061 ccactaatgg tgcagttgca aatccaaatt cttccgctcc aagcgcacat gcgtacgcta
    12121 catctttacc agttaataac ttaccgtctg tttctaactt aacacgactt cttaagtcat
    12181 ttagttttaa tgtttgatgt gtttctgcta aaccaatctc ccaaggaaca ccggcatgct
    12241 gaatactcgt tttgggtgaa gcccctgtac caccatcgta accactgatg acaattttat
    12301 ctgcaaatgc ttttgccacc ccagatgcaa tggtaccaac acctgttttc gaaactaatt
    12361 ttaccgcgat atctgcatct ttattcgcat ttttcaaatc atgtatcagt tgcgctaaat
    12421 cttctattga ataaatatca tgatgtggcg gtggtgaaat cagaccgata cctggcgttg
    12481 accctcttgt cttcgcaatc cacggatata ccttagtacc aggtaattga ccaccttcac
    12541 caggctttgc accttgcgca actttaattt gaatttcttt ggcatgttgt aaataatcac
    12601 tagttacacc aaaacgccca gaagcaactt gtttaatcgc acttactttg ttgcttccat
    12661 caacttgtac ttcataacgt tttgcatctt cgccaccttc accactatta ctctttccac
    12721 ctaattggtt catggcttgt gctaacgttt catgtgcttc cgctgaaatc gatccataac
    12781 tcatcgcccc tgtattaaag cgtttgacaa tgtcacttac cggttcaact tggtcgatgt
    12841 caatcggtgt acatgcttta aattcaagta aatgtctaat gtgatctgtt ctatttttgt
    12901 tcaccgcttc agagtatgct ttaaattgcg catagtcatt ttctttacat gcgtgctgca
    12961 ataagaaaat agattccgga ttaaaagcat gatgttgacc ttgttgtctc cattggaatg
    13021 tactacctga tgcaagataa ttatcatcac tttgttgacg tgctttattt tcagcatcaa
    13081 tttgatcaat cgaaatacca gataacttag actgtgtccc agtaaaataa cgatcaatca
    13141 catcatgaga caagccaatc gcttcaaata tttgtgcccc ttgataactt tgcactgtcg
    13201 aaattcccat cttagccatt actttaatga caccttctga caatacatcc gtatatgtct
    13261 taacattatc gacaacggtg ccttgtaacc cttctgtcaa tgtcagttgt tcaactgtac
    13321 gttgcgctag gtatggcaca attgcattcg cgccatatgc gagtaaacaa gcaacatgat
    13381 gcacttctcg tgtctcacca gatttagcga ctaaacttgt agacatacgt aaatctgctt
    13441 taataagtaa ttgatgcaca tgacttattg cgagtaacat cggcattgca aagccattgc
    13501 tatcaactaa tccactatca tctaacacta gaatttgagc gccttgcttt acagcattca
    13561 ctgcttctcg gcctaatgct tctaacgcat cttccaaatc cccttcatat accgttgata
    13621 aataagttaa tttaaaatgt tcctgatcaa tcgctgctaa gtgtgattca ttcaataccg
    13681 gccttttcaa ttgaatacga tctaaaaccg tttcgtcagg tgctagtaag ttaccttcgc
    13741 cacctaaata agaaagttca ctcgttacga ttttttcacg atacgcatca attggtggat
    13801 tcgtaacttg tgcaaacagc tgtttaaagt aattaaatag tgattctggt cgctcgttca
    13861 acactgcaat tggcgcatca tatcccattg caccgatagg atccttctta ccttctacaa
    13921 gttcctgaat atacttatga atctcttctt tcgtgtatgc aaactgacgt tgtaatttaa
    13981 ataacgtctc atctttccat tgcgaatctt gatattgtat attttcaaaa tcaaagtcaa
    14041 ctttatggtt atcaatccac gctttatatg gtaattctcc agcaatcgca ccttttaaat
    14101 cattattttc aatgacttta ttctgtttaa aatcaacaag caataacttt ccaggattca
    14161 attgaccttt aaaagcaaca ttactttcag gtacgtccac aacacccact tcagatgaaa
    14221 agacaataaa gttatcttta gtaatcgtat aacgacctgg acgtaatcca tttctatctg
    14281 taagcgcgcc aagtttgtca ccgttacaga acgaaatcat tgtaggacca tcccacggtt
    14341 ccattaaata actataaaat tcataaaacg cacgtacatt tgcatcattc gcttcattat
    14401 ataaccaagg ttcaggtatg agtaacatcg ctgccttttc tggctccatg gctaacgata
    14461 agaactctag cgcattatct acaatggcag agtcactacc atcctcatcg acaatttgaa
    14521 acactttatg ttgatcctcg ccaaataatg tttcgattaa tttatgttgg cgtgctcgca
    14581 tccagtttac attaccttta atcgtgttaa tctcaccatt atgcattaac atacggttag
    14641 gatgtgccct tttccaactc gggaatgtat tcgtactaaa tctcgaatgc actaacccta
    14701 gctttgattg atataaatca tccgataaat ctgtatatag ttttttaatt tggtctgatc
    14761 gtaaccaacc tttatataca attgttttgc gtgataagct cgtaaaatac aattctaaat
    14821 cgcactgagt cgaatagaac tctaattgtt ttctcgctaa aaacaaacgc ttttcaacat
    14881 cttcaatgtc cctaatatca ataaacactt gttgaatgac tggcatcgta tctgctacat
    14941 gtttagcaat ggcatcttta ttaactggta cattacgata accaagaatt gataaccctt
    15001 cgccttcaaa atatttttta aaaactactt catgttcaga acctaaaatg cgttctttgg
    15061 aaaaaaataa ccccacggca tattcacctt cacctgggat atcaaagtcc gttacatgtt
    15121 gtttgaaaaa tgcaaaaggt atttcagtca taatacctgc gccatcacca gtgatgccat
    15181 ctgcgccgac cccgcccctg tgatctaagc gtcgcaacat ttcaagcgat ttatcaatga
    15241 tgtcgtgaga ccttttatta tccatattcg cataaaaacc aataccacac gcatcatgtt
    15301 cctcacgata gtcatataag cctttaatta atttctcatt gtgcatgatg cacacccctt
    15361 ttttagaata ttctgatatt agttattaat gataattact ataatctaaa aaatttatct
    15421 tttcaatata taaaatagaa gatattcatc taaaaaatag aacatttatc aataatattg
    15481 ctataatagt tacaaaacac tcatttttgg aggtgcctat atcgctatgg aaattaaaca
    15541 attaagatat tttatcgagg tcgctaaaag agaacatatc tccgaaacag cacttgaact
    15601 aaacattgct caatcagcga taagccgtca aattacatta ttagaacaag aacttaatgt
    15661 gtctttattt aaaaagcaag gacgcaatat tacgctcaca tctgaaggga aattattatt
    15721 taatgaagct ctgcgtatta ttgaacattt agatagcacg attgaacaat ttcaaagcca
    15781 tggattaact aaaaataaaa gtatctacat tggctatgat gagagtgacg tatcccacat
    15841 gttactacca cttatccaaa cgtttcattt acaaaatgat acacatgtca taccgagctt
    15901 attagaacat gacacaatta taaatagcgt attaaatggg aatatagaca ttggtttcac
    15961 ggaacttaca ccagaaatac gtaaacacaa acaattacat atgctacctt tattcgaaga
    16021 gcactatcat ttatatgcgc cttcagacga tccaattacg atggcgacac acccaccatt
    16081 aattcagttt gagcatagtc atatttattg cttggcacca tttgccgaaa ctgtaaaaaa
    16141 acaattgcgc aagattacta aatcagatgt atacacaatc agtagccaac cacttgcgca
    16201 gtatctcctt cgtcaaaaag aaggttacat catttcatca caaaatatac atttaccaga
    16261 atctaaatca tggatagata taaaattaga tcatactgaa ttaaaaagaa cgatatgtgc
    16321 tattacaaag gagccttata caaagagtga tattggcata ttattaacac ttattcagca
    16381 attaatgact aaaacgtcta cattccatta agtaattcga tgctatcaat gatgattttc
    16441 gttttacact tagcagtaat atctttgaaa ataaaaaatt aagggcgtat aatcaccata
    16501 ttaattcatt catagaattg gggttttgtt tttgaaatat agaaatttat ttacaaagcc
    16561 atttaatata attttaggtt tgttctgcat cgcattcatt ggtgctattt tatttacatt
    16621 caataatgag aacttttaca acaaaccaat tggtcaaatt atagatgtca aacatgtttc
    16681 ttcgacacct acaaaagatg cacaaaataa tcgtgatatt aaatataaaa atcaattaaa
    16741 agtcaaaata ttaaatggtc aatttgctgg agaaacgacg acgattaatc atcagtacgt
    16801 caaatcgcaa gcagattctg aggcatttcg cacacacgaa aaagtattat tacatatttc
    16861 taacaagccg agtgatgctt atattataga aaagaagcgt gataccttaa cggttatcat
    16921 cactggtttg ttcttattaa ctgtattatt ggttggtcgc aaagtcggac tgcaatcgat
    16981 tttatcgtta atcttgaatt ctattgccat attaatcgca atttacattc atattcaaca
    17041 tagcaatatt aatttattct tactcatgac cattgccatg atctgttcaa caatactcac
    17101 tttattgctc gttacaggct ggcatatgcg tacactcatt accatagcca gtacaattat
    17161 cggaactttt ttgagtatag gtttaacaga attaatcata tatatggcag atggtaaagg
    17221 cattaaatat gaaacgatga actttttatc attaccaccg aaagatatct tcctagcctc
    17281 agtgttaata ggttcacttg gcgctattat ggatgttgca attacaattg ctagtggtat
    17341 gcatgaaatt ttacagcgta cgccacacat ttcaatgagg agatgggcgc ttgctgggcg
    17401 aaacatcggc caagatatta tgggaacaat gactaacatc ttattattct catatttatc
    17461 cggtgcacta cctatgtttt taatcttttt aaaaaatgcc aatacagtga cttatactat
    17521 ttcgatgaat tggtcgttag aaattgcgcg cgcactaaca ggtggtatag gtatcgtact
    17581 tacaattcct atcacaatat tgtttatgga aatatttgaa acattaagga gggcgaaaca
    17641 atgaatgctg tatttatatt ggcgttattg ctcctctttc tgatgataat tttcggaggt
    17701 aaaaagggat tcatctctta tttaacccta tttttaaatt tcgctatttt aattattagt
    17761 attgtattga ttatttttgg cgtaccgatt tatgtcgtca cattcttttt ctgcattatt
    17821 attggcgctt gtaatttatt cgtactcaat agttacaatg ttaaaacaca agctgcattt
    17881 atcagcacta tcgttacaac aatattgttg attacactca tttatttatc ggtctacttc
    17941 ggtcatctac aaggcttttc aaccgaacaa caagatgaaa catatgtgta ttcaatgaat
    18001 attggcattg atatggttca atttatggtg ttcactatta ttcttgctgt tattgcagct
    18061 gtgatagatt taacaattac cattagttca cccatttatg aattgcatca agtgaatcca
    18121 acgcttacgc aatacgaatt attccaatct ggcatgcgtg ttggtagaga aattttagcg
    18181 acatctgcga acacgattta ccttgccttt tttggtggcc aacttgcttt atttatttgg
    18241 tttttcaaac ttaaatattc atttggtcat attataaact cgaaaatatt tgcccaagaa
    18301 tttatttcta ttattttagg cggtattgcc gttgcaatca gtattccgat tacggcttgg
    18361 attacggcat ttttaataaa aagaccatct cgacagcgca aaaatgatga gtctatcaat
    18421 taatcgcaac tagcaatata acttaagtta agcgacctgt ggatatatgt caattgttca
    18481 ctaggtcgct ttttactata gtaaattgtc cgatgatcta taacaaaaag cacctttatt
    18541 catcattaat tgttgtggtc gacgatgagt aaagatgctt ttaaatttaa tttattttat
    18601 agttaacgcc ataatatgaa caggtcttcc gacgaacata ctatctccca tatattgata
    18661 tccttgttgg cgataaagtg ccatggcatg tggattgtca gtattaaccg ttaacacaat
    18721 ctcattaata tcctgaaatg ttgaagtgat aaatgacgcc aatttttcca ttaccacttt
    18781 acctattcct ctattacgat aacgttgatc aacactaaat gacctgaaaa atactgcatc
    18841 ttggttatcg ctaaatggtg cgaccccttt tccacgatgt aatgtaaaaa aggccacaca
    18901 ttgcctgcca tccattacaa cgatgggata cctttcatta tcagacattg cagctgtaat
    18961 attttcctct ggcgttttaa caaacttcaa atcactttca cttaattgaa aagcttctaa
    19021 catcgaataa tcttgtggga cataatgtcg taacatgatg gtgtcattat ttgtcatgtt
    19081 taccaccata agtttcaatc caaactttga cagcaggcgc aatcaacgca tcattatctt
    19141 tatcaatcca gcgtatatcc gtaatttcat tattgatagt tacgttttcc caatcgattt
    19201 tggttaatgc gcgaaatcca tttaactcag ttaacatatc ctgttgtgga tatgcaggac
    19261 ctacaattgt cccgacatat tcaatttcat ccattgttaa tgttaaattt aattcttctt
    19321 ttacttctct taacagcgcg tgtacttgtg attccccttc ttctatttta ccacccggga
    19381 aataatactt ttcgcgattc cttacttgaa caagcaatat tttatcagct gtttcttcaa
    19441 ctaaacagac acatttaatc atctttgaca ccccaacttt gtgaaatcaa tttttcaaat
    19501 tatactgtac aattatgtta tcatatatga gtagttatag cgcaaaacgt tagcaattca
    19561 gcgcacccaa cttttcatat aaacagaaga tactaggggg aattattatt atggcaaaac
    19621 gttccaaatc acaacgttta tcaagtttac taaatgtcgc aggtttcata gtcgacggct
    19681 acaatggcta taaatatcat gctaaaaata aaaaattagt atatctttca ttaggtttaa
    19741 gcactgtagg aaccgtgtta gacttttaca tttcaattaa gtcaccacgt aagttcaaaa
    19801 aagcagtggc agttgttact ttaataacaa acggtgctag attatttaca agcattcgca
    19861 aagtaaaaca tgaatactaa ttcagaaaag gattggtcga acatagaaca tgaagttcat
    19921 tcgaccaatc ctttttcata tacaaaaatt cctattacaa caatcacgac tttgatagcc
    19981 ccgccataaa atttaggatt caatccaact tttcagcttg tgaaatgtaa taggaattca
    20041 ttatatattt atatacgtaa gactttagtg aatatatcta taattattta cttggtaagc
    20101 tggtaccgtt ctgtaagtta aaatacctgg tgcagctgaa tagttcattt ctgaaactaa
    20161 aatactacca tcgttattta cacgttctac aaacataaca tgaccatagt aacctacatc
    20221 tgtttgagcg atagaaccta cagtaggtct attgtcgata gtgtaaccat ctgctgccgc
    20281 tgcgttatcc cagttattag cattccacca ataagtacta atacctttac caatttcagc
    20341 acgacgatta aatacatgat atgtacattg accccatgta tataagtttt ggtgactgaa
    20401 tactggtgta ttgtaacctc tatttgtcgt tgttgcagat cctgagttcg tagatgcatt
    20461 accagttact ttcaattttt gacccggata tataaagaaa ttatttaagc catttaagct
    20521 cataattttt tgataagttg taccatattt tgatgcaatt aatgacaatg agtcacctgc
    20581 ttgtactgta tagtatgatc cgccacctga gttcgttgat ggacggctac tattgctcgc
    20641 agcgttactt gagctagcag tacctgatac ttttaatttt tgacctggat aaataaagaa
    20701 attatttaaa ccattaagtc gcataatgtt ttggtaagtt gtaccatatt ttgatgcgat
    20761 taatgataat gagtcgcctg cttgtactgt gtagtatgat ccgccacctg agttcgttga
    20821 tggacggcta ctattactcg tagaattact tgagccagat acttttagta cttggtttgg
    20881 gaaaattaga ttagatgtta aattgtttaa tgactttaat ttagcaatcg aaatcccata
    20941 cttatttgaa attgcccaca ctgattcacc cggttttact gtgtgagttg tagccgcatt
    21001 tgcttgagtt gccgcaacag cgctaatcgc gcttgtccca ataatagctg caattacttt
    21061 tttttgcact ttaaaatcct cctcttgctt aactttccta acattctttt atccgaattt
    21121 atgaatacta catcattata cgattttatt atgtataata ggttgatgtt tgatgacatt
    21181 atgattacaa aaaaatcata tactgtatca tcaaatttta taattacccc ttaaaattat
    21241 tacaacttat tagattttac aatatctaaa ttattacaat ttcataatat ttcactataa
    21301 aatgattaca atccctttct ctattggaaa taattttatt ctccaacaat aacgccctac
    21361 aaacataagc atgaactttg cttgtaggac gcaatataat ttattttgct aatgacgttt
    21421 ctattgcctt aatctcatct ttagataaat taacaggttt ctctccatct ttgacatctt
    21481 cctgcaacgc tttttgagct tcttttgaat gatacaattc tacgatttta gcatatgttt
    21541 tgttatccaa gtctttgtca ttaactgcaa caatattaat atatggcttt actgcatctg
    21601 aatttgattt ttctaaaaat atcggatcat ttttaggatc tttacccgct ttagttgcta
    21661 caccgttatt aataactgca atatcgacat cagataaagc acgtgcagtt tgttgtgcat
    21721 ctactgcagt aatttttaaa tgttttggat ttgacgttat atctttcacc gtgcctgcta
    21781 atccgaaatc ttttttcagt tttattaaac cagctgcttc taatagttta agtgcacgtg
    21841 cttggtttga cacatcattt ggaatgacaa ctttagcacc atctttaacc tttttgacat
    21901 ctttaatttt atctgagtaa atgcccaacg gtgctaaaac tgttgtactt aatgctgaaa
    21961 tctttgttcc tttatgcgcc tttttatatt gatctaaaaa tgcaaaatgt tggaatgcat
    22021 tcatatcaat atcaccatca tttaatgctt tattcggtaa attgtaatct gagaagtgct
    22081 taatctccac atcaatatca tcttttttag ctaattcttt aaccttctcc caagccttag
    22141 tgtcatttga tgcgacacca attgttactt ttttatcatt gttaccacca cacgctgcta
    22201 ataatacaag tgctacgata actaacccaa tcaatctttt cattctatca attcctttca
    22261 aaatcttcac tatatatcat taatgtctac gtatgaatct agctagaaca ttccctagcg
    22321 tttgaatcac ttggacaata atgactaata caataacggt aataataatg accgtcgtat
    22381 caaatctttg ataaccatac actaaagcta agtctcctat accaccaccg ccaacagctc
    22441 ctgccatcgc cgtacttcca ataagtccaa taatcgcagt ggtaattgct aatactaacg
    22501 aacctaaagc ttcaggaatt aaaaaatatc taatgatttg tagtggtgaa gcgcccatcg
    22561 ctttcgccgc ttcaataatc ccctcgtcta cttccaataa tgagttttca acaagtcttg
    22621 caatgtaagg tgccacatat actgttaaag gcacgatggc agcagtcgta ccaattgaag
    22681 tacctactac taattttgtg aatggcacaa tcgcaattaa caaaataata aatggtagtg
    22741 accttaaaat attgattaaa ggatttaaaa cttgatgtat cactatattg ggccatatgc
    22801 cttgttttcg agtaattacc aataagacac ctaatggaat accaatcact gctcctaaaa
    22861 ataaagcaat agataccata tatagcgttt cgtacaatgc ttgtaataac tgtgcactgt
    22921 ctaaatcaga accaaacata tgttaatgca cctcctcaaa ttgaatattt ttctctttga
    22981 aatattgatt tattgccgtg tcttcaaatt gttgatccat attaaatcga agccacatat
    23041 aacatacggt gttaccttgt atttctgaca tagatgaaaa taaaatttta acctctctgc
    23101 cacaaatttg aatcaagtca tttataatcg gttgtgtcac ctgagtttcc tcgacgaaga
    23161 ttttataatc tttaaaatcg ccaacttgtt cgtcattcaa tcgacgaatc aatgatgtac
    23221 ttggctcagt ctgtataact gtagacacaa aattttgagc aatcgtcgtt ttaggatgac
    23281 taaacacctc tttaacagtt cctgtttcaa ccactttccc cttttccatt acagcaacac
    23341 gattacaaat gtctttaata acgcgcattt catgtgtaat catcataatt gtaatgccaa
    23401 aggtttgatt gacattcttt aataacgtca atatcgaagc agtcgttgct ggatccaatg
    23461 cgcttgttgc ttcatcgcat aggagtattt tcggattagt aacaagcgct cttgcaatag
    23521 ccaccctttg cttctgccca ccagataatt catcaggaaa ttggtctttt ttatcactca
    23581 atcctacaaa ttcaagcatt tccgttactc gttgcttaat ttctgttttg cttttcttac
    23641 ttaaaatgag tggcattgct acatttttaa atacggtagc tgaatttaat aaattgaaat
    23701 gctgaaatat cataccgata tctttcttaa tatccctcat cattttatcg ctataattcg
    23761 taatatcatg tccatctaca atcacttgtc cattcgaggc agcttcaaga tgattcacga
    23821 gtcttaccaa cgtacttttt cctgcaccac tatatccaat cacaccaaaa atatcattgc
    23881 gattgaccgt aaatgatacg tccttcaaag catctatttt ttgcttcttt ttattaaagg
    23941 tcttacttac ctgtcgaaac tcaatcatca tcgcccctcc tacaaattct tattaatcca
    24001 ctaggaataa gagcattata tgtaaaattg catatatcgt caatacaatt tgccgaattt
    24061 tctaaaaaat taaaaaataa gtaattcatg tgacaatgac gaattgtgag actactatga
    24121 catttatcaa attaaatcca taaaaatgtc caccaatcct ccacaacgca attactaaat
    24181 attaacatcg cacaaaaaag cactagcata ttcaagaaca acaaacgttg aactcaaaat
    24241 atatgccagt gctgctatta tttataaagt atctagtgct tgttttaaat catcgactaa
    24301 atcttcagta tcttcaatac ctacagaaat tcttacaagt ccgtctgtaa taccttcttt
    24361 agctcgaata tctgctggaa tggatgcatg tgtcatcaat gcaggtactg aaattaaact
    24421 ttccactgca cctaaacttt cagctaatgt gtaatacgat gttgctttaa tcaattgttt
    24481 ggcactttct gtatttttca cttcaaatgc aatcacacct gtatggccat ccgcttgagc
    24541 catatggaca tcatgattta aatgactttc aatacttgga tggaacactt gttgcacagc
    24601 tggatgtgct tgtaacattt taataatttc aataacgctg cgattaattt gttccatacg
    24661 taaacctaat gttttaatac ccctcacaag taaatagcta tcttgaggtc ctaaaatgcc
    24721 acctgttgaa tttgaaataa atgctaaacg ttctgcaagc ttgtcatccg atgttgcaac
    24781 taaaccagca acgacatcac tatgtccacc taaatatttc gttgcagaat gtaagacaat
    24841 atcgatacct aaatctaatg gattctgata ataaggtgtc ataaatgtgt tatcaacaac
    24901 tgaaatcaaa ccgtgttctt tcgcaatttc agcagacttt ttaatgtcag taacacgtaa
    24961 taatggatta gaaggtgttt caataaacaa catctttgtt gttgggcgta tcgcttgtac
    25021 aattgaatct gtatgcgttg tatctacaaa atccacttca atgccaaatc gtgtaaatac
    25081 ttttgtcaat gcgcgataag taccgccgta tacatctgaa tttaaaataa tatgatctcc
    25141 tttgtccaac agcataacaa ctgcactgat tgctgcaaca cctgaactaa atgcaaagcc
    25201 atgtttgcca ttttctaatg tcgcaataac gctttctaca gaacttcttg ttggattcgc
    25261 agtacgagaa tattcatatc cttgacgtaa atcaccaata tcatcttgta aatatgtact
    25321 tgtttgataa attggtgttg taacggcacc tgtataatcg tctgttgtgt gcccaccatg
    25381 aattaattta gttttcttgt tcattattat tctcctcata attaaatatt tgcttagaca
    25441 tatatcgatc actaccatct ggaaatacga caacaatcgt accttcagat aattgcgctt
    25501 ttaaattcaa tgcaccttgt aatgctgcac ctgaagaact gcctactaac aacccttcat
    25561 ttatagccaa acttttgaca tttcgaaagg catcttgatc tttaatcgta aatatcccat
    25621 ctacaagacg tctctctaaa aatatcggcc atttctcaga accgatacct tcagtgtcat
    25681 gtgcatgagc tggccctcca tttaacacgg acccttctgg ctcaacggca taacattgca
    25741 cgtgatgttg ctttaaataa cgtgcggtac ctgtaaatgt accgccagag ccaataccag
    25801 ccacaaaata atcaatttgc tgtaatgctg aagtcaattc gggtcccaat gtatgaaaat
    25861 atgtatccgg attatgttcg gattcaaatt gattcatata aacggcacca tatttttcag
    25921 cataggaacg tgcagctaat tgtgccccat gcataccttc agactgactc gtccttgaaa
    25981 cttctgcacc aagcgctatc ataatattaa tcttttcttc tgaaaaacca tacggcgcaa
    26041 agatcttaca tttcaaatga tgtctattcg ctgcaatagc taaccctatg cctgtattac
    26101 cagcagtcgc ttcaacaata gtttgacctg cacgcacacg cccttcttga attgccttct
    26161 ctactaaata tttcccgagt ctgtctttaa cactgcctcc aggattccat tgttcaagct
    26221 tggcataaat tttaacttta tcatcactat aatgttctaa cagtactaat ggtgtattgc
    26281 caattaaatc ataagtaatc atagatgcac cctcatctga catgccgatc aaatgaatga
    26341 aacctttctt catgtctcaa ttttaattct tacttttcag ataagaatta taaacgacat
    26401 tttgttattt tgcaattatc taagtttcga ttaattcaga accagtacta aattttcaat
    26461 tccaaacaaa aaaacacctg agcaacacaa atacttgtgt gtcagatgct tctatatatt
    26521 aactaaataa ttgcacgata aagactaaaa taataacgac aggcatcgca tacttaatta
    26581 agtaatacca accactgaat aatctaaatc gatctttacc aaaatattgt tgtaataatt
    26641 ttttatctaa taattgtcct acgacaagcg tagtacctaa tgcgcctaat ggcatcaata
    26701 cattcgaaac gatgaaatcc atattatcaa aaatcgttcc cgcaccgaat cttacatctt
    26761 ttaagatacc aaaagataag gttgctggaa tactaatgat aaatactaaa atactaccga
    26821 tcactgcgac ttttttacgt tttgtattgt cattcttcgt gaagttagaa acatttaatt
    26881 ctaataaaga aatagatgac gttaaagccg caaataagaa cagcactaag aatcccaaat
    26941 agaataatgt gcctagatgc atttgactaa agaccattgg cagtacttta aataataatc
    27001 caggcccttc ttgtggttca tagccaaaac tatgtaaagc cggaaatata gctagacctg
    27061 ccaatacaga tacaaagata ttcataacaa cgatagaaat agctgatgac ttaatcgtca
    27121 tgtctttaga ggcataactc gcataagtaa tcatacctgt agttcctaat gataacgtaa
    27181 agaatgattg acctagcgca aacaagatgc catcagcagt aatctctgat actcttggtt
    27241 gtaaaataaa tttcacacct tctaaggcgc catctaatgt taaagactta atcacaatga
    27301 cgattaaaaa gacaaacagc aatggcatca taactttcga tgccttttct aatccttttt
    27361 caacacctaa catgacaata atcatcgtag cgaatatgaa tataccttgc cctagaacgg
    27421 ttaaccaagg atttgatatt accgcttcaa aattcatttc ttggagatga ttgatgcgtt
    27481 gaaatataac taattgccat aatacttgtc cgatgtaaat gacaatccaa ccaccgataa
    27541 cactatagaa gccaaataaa ataaacactg ccaaattacc gttccagcca atgatattga
    27601 gccatttttt accagttaat ttactatata tttgtgttgt atatgtccgt cccattttcc
    27661 caacagtgaa ttccataatg agtaatggca acccaacaaa aatggtgaat attaagaaca
    27721 tagctagaaa ggcaccgccg ccataaatcc ctgccatata tgggaatttc cacatggcac
    27781 caagaccgat tgcagaaccc gcactagcta aaataaatcc agttgatgac ttccattgtg
    27841 attgttgtct tttcatcatt cacatctcct ttaaagcgtt aaagcgtcta cgtgtttaag
    27901 taacactcta acatgtatgt aaataacatg caatacacct gcatcaaaat tgtttcatta
    27961 atacggtagc acctggattt atcataatat aatcaactct tgtaatgtgt tgtcattacg
    28021 ctacattaaa ttttctggtt caacgctgta tacgtgcgcc aatatctcgc cacatacaac
    28081 accatctata tccccaacat ctcgccacat aacaacttca ggtgcaatgc ccataaaatt
    28141 tccacgacat tacaatacaa atcaacttct ggtataactc cataatattt ccacgacatc
    28201 acactacaaa ttaactttag ctaagttccc tatactatca cgccacaaaa aacactcaaa
    28261 tgacaaatat aataacatcg tcactcaagt gttaattttt aaaatcttca attatgttaa
    28321 agcattgaaa aagcgaataa cattttcttc taaaatatct ctgccttctc catgcgtcat
    28381 taaatgatgc gaatccttca gaccatttaa ttctttgtct actgatccta aatgttcata
    28441 aataaaatgt gcacttgttt cataggatgg cgcatcttta ccaccaaata atatattagc
    28501 tggcatttta ataacattta aatttgtcat gatatcatca ataaaatgtt gaaacttcgt
    28561 aatttcacct tcataatcat caattgctgc taattgcgca ctcgatgctt gctgatctaa
    28621 atttaaaata ttcgacatgc gttgactata ataaactaaa tgttctatta agccatcgtc
    28681 actcttttcc tttggtgctg acatgacagc gatacgtttc aaaggatagt gttgcgccaa
    28741 ttttaatgtc attaatccac ctaaagacac acccgttgca ctgatagatt cataaccttc
    28801 attgactaaa aattggtaag ctttctcaac ttcttcccac caatcatcta cattatatgt
    28861 catgaaatct ttcaacaata aaccatgacc tggataattc ggtgcataac aactaaatcc
    28921 ttggtcattt aactcagctg caagatgctt cacatcccga tttgtacctg taaatgaatg
    28981 taataataat atcgcatgtc catttgtgcc ttttaaatac gatggactcg gtgttttaat
    29041 tctcattttt ctatatacct ccactatgtc taaagatgtt tgctaaacgc gttgtcgtcg
    29101 atgattaata aagtatgggt ataccattaa gaataacgct acccaaatga ttgctagtga
    29161 cgtgccgcca atgacatctg aaaagtaatg tgcatgaaaa taaaggcgac aaaataatat
    29221 gctaagccat aatattccca taaccaacgc actcaacact tttgttattg tcttagcagc
    29281 aagtgaaata ataatgatca ttaaggcgaa atataataat gtgctggcgt tggaatgtcc
    29341 actcggaaat gaaaagcctg tatcaacggc taaatgatta tatggtcttg gacgtaatac
    29401 agtatcttta attaatttgt tcatgatgac acctgaaacc aaatatgtca caaaccaaac
    29461 cgctaaatgc ctctgtttaa taaacagtat gattgtgacg ataatggaaa tcaacacgac
    29521 acctttgaca tctccaattt ccgcactaaa cgtcatatag taattaaaca aattgttaac
    29581 atactgacgt tgtggctcac cgaaataatc tgtaaaccat gttaatgatc ccatatctat
    29641 atttttaagc cattcttgat ttgtcactac actgtaaaac atacctataa atacaatcag
    29701 cgcgattaaa aataaaggca ctgtcatttt cggtgatgtt aattttttat ctatcatctt
    29761 acaatctcct cgtatcatca ttttcatttt acaaatgtta tccataatat caatgtgcca
    29821 caaatttcac tttaccgaca atatcaaaat tataaagttc atattgttat gtatattgca
    29881 aataaaacat tgtataattg aaataacaat attttgctat tttcaattta gtacgattta
    29941 tatttattat acagaggggg taaggcgtat caatagagtt atttttgtct atatagcgtt
    30001 aatcattaca ttagttagat tctttacccc tattcatcca tcattttcaa atttgattta
    30061 ctggatattt gtattatatt ttattcctat tatactatgc gttatcggtt tcaaggccga
    30121 aaaacttatt gcaacaatgg tcattatacc taatttttta ggaatacttt atcgattata
    30181 tgcctacgtc acacatatac tctttatgta aaaggatagt gggcatgtct cgcaacaaat
    30241 atgcttgcgt cgacttgtca ccattcgcaa attttatgac atacgccttt cacgggctat
    30301 attcagaccc acgcattcat ccacgtaata aacacatcat gtaatagaaa aacagcacac
    30361 ccaaatatat ggcgttgcgc tgtttaacca agcatacttc tatagcttta ataagccagc
    30421 agaagcatac ctaaccttct taaatatgct tttccaaatt atcctcaagt ttgaatacga
    30481 taatacgttc acctgtaact gtacttaaat cactatggaa gctcatcact ttgatacctg
    30541 taattttaaa aatgatatca ttcaaatctt gctcaccgga ttcaactaat tcagaacgtg
    30601 ttcgtttaat atttaataat ccttcattcg tactacatac acgatattca gctggcgtta
    30661 agataccttg taaactaata atcaccatat ctcttaaaat gtctgattta actgacaagg
    30721 aacctctgcc aaggaaatct ttttcccatt gcgtaatggc tttactgatt tcagcttcga
    30781 tttcaccttt cgttcttttc atatcactaa ctccaataat attaaaattg attacttcat
    30841 ctttgtatcg ttatcacgac atcaactatt acattaagtt tatcattttt agtatatttt
    30901 aagaagctag aacattgtag atatgatgat atattagtta cttagcatcg caacatatca
    30961 tcgttaaatc caacttttaa aacgcccttc ctcattaacg ctcattaaac gcagccaatg
    31021 attagacacc ttcctagcga aatgctcatt attcgcgagt agtcttgcta caacatagtc
    31081 gggtgcctga ataacgacaa gtaaacgaat tggcgaatga tacatcgtcc gatcagcagc
    31141 cataacagat tgccatgata agccatacat cagatcactc gcattacctt gcatgacacc
    31201 aacacctgac gtgacggttt gtgtcgcttt attcccactt ccgtaaaaat gcggcgcaac
    31261 tgtcgacgca taatattgta aattaatcca ttgtgccaca agtgccggac cagaaatgat
    31321 ggtatttaat aatgtgccat ctttatcttt acgccaatca taattgtgta aaaatgtccg
    31381 cccttctaaa tcaatgcctt ttgttaattg gcgtcgtcca attataaatg atgcattttt
    31441 agccaatccc cattctggac gtacctcact ccaatcactc gcaaaccgct gcgcttcttc
    31501 cactggatga ttcacacgac caatcgttgg cagtttgtcc aaacgttcgc gattcgcgtg
    31561 ttcagaaatc atcggcatcg cgtcattcaa tgattcatat gcatctaaag caatagaaga
    31621 taatgtgtct ggcacatata cccatgccaa cgtatcagta gacgtatgat gttctgctac
    31681 cgcaaaaaca gttgtctctg gaatatacac acctgattgt tttaatcctt gtctgacatt
    31741 tggacgatta catatcatcg ctaataactt agcattaaaa ccgcttgatg cgccaccaca
    31801 agccccacat tcaagtgatg catgatgtgg attattgtga gaatgactag catgacctgc
    31861 taacacaacg aacggcgcaa atgcttcggt taaatccatc aatttcaacg cttgtaacgc
    31921 gaaatcaatt tgctcttgct cagtaaatcc aacaggtaag tctgatgttc ggtcaaactc
    31981 acgatcaatc gtcaactttg tttcaggctt tttcaaccac ttttgtttta ttttttgtaa
    32041 agacgcgcga ctttttctag gcataatcga attgacaatg gtacttaagc ttaaaaatgg
    32101 cccacttaat tcaggcaata acagactagg catgacatta tttttcatca atttaaatgt
    32161 gtaaaacatc gatgacattg tctgttgctg ttgtcgataa acattcatat cgtagcggtc
    32221 tgcaaattct ttaatgcgat atgccggcgg taccatgaca ggtaatgaat catgtttgaa
    32281 ttgttcgtct acggcatctt tttgaatagg taatccaaag aagcctgcaa taccaatcgt
    32341 ttcaaagggc cctgctgctt cgatatgtct acgaaatggt tctgaacgaa catctataca
    32401 aaatgcaatt tgcgctttcg ttgatgtgcc catctgattt agctcgctat tattttcatc
    32461 aactgcttgt gtgtcattta acaatactga atgtggctga ttagcgttat cattttctga
    32521 gacatttact tggtttacat ctaatgcgcc cgccacactt tcatgacctg ctttaatttt
    32581 ttgttttaac tgagattcgt atgtcatttc ccaggcaatt agccataaat ttttaaatac
    32641 atttttattc atagttgctg caaaatgaat aaacgtttga atttcattga cgtcatgttg
    32701 tagtaataca tcgctaggca tatcactgta gtaacaccat gatgcaacag tttgcttaaa
    32761 ccaattttcc gatctacttt cacaatcttt agcgactgac ttaaactcat caccaactag
    32821 caattgttcg acaactaacc gaattgccaa ataatccgtt aacaaatgtt gttcaaagta
    32881 atgctgttgt gaacggtaat acaacatacc tgcccaaccc ggtaacgcca aaagatgtcc
    32941 ttcaacataa gcttggtagt cttcctgatc tattgaaaaa tgagttaata ctgactctat
    33001 cgtcatttca ggatcattgg gtaagccttt aatcacttgg cgctgtgctt tagtaaaact
    33061 atggtcatgt tgcgctaaat gcaaccatgc atggtaaaaa ctttgctcac gcttcggcat
    33121 tgtccaactc gatagaaatt gatcgatata aagtttcgtc catttaatca tttgacgatt
    33181 cacttgttcg ctaagtggct caccttgttc atctattatt gcatcactca tcggacgtac
    33241 atcatagtga tgatatgatt cagccatatc acgttttgat ttttctaata gtagatcagc
    33301 aacaacatca acatttgaat gattcatata tgatgcaggt acgtctttta atgttttaat
    33361 gttatcaata taaagattga tgtagtgttg cgggatattg tagtgatgtt caagtaacat
    33421 atcagtaaca agttgattaa agacactttc atctaattca ccacgtgcca cagcgctttc
    33481 tattaatgct ttatttggga aaatatccac atctcgaaca tcacgtaacc attttgcgac
    33541 atcttcaaac gtatccgctt ctaatccttc ccatggattt cgtgctgcaa aaatcgaaat
    33601 tggtgataat ggtgtaataa cacgtttcgc attttcaatg actgaattga tatttaactg
    33661 tgttgtcata cctttcacct cctataaata cttcttcaaa taattcggat gactttctat
    33721 cgctttcgag cgtgcttcac ctagattaac taaccacacg tacaataccg caaaagcctt
    33781 agagtatcga tgccgcgcca cccaaatact taataaactg ccaaagatta aaataacaac
    33841 actaatgatg acactcactg taggcggcgt tgtcgcatgt gttgttatat tttgtaatac
    33901 agcgtaaaaa taattatgtg tgatgacgta gataaatgtc acgattgcaa tcaaaatcat
    33961 accaacaaga cgtgccatgc gtcctttact aaaggctacc atttgattcc aagatacaag
    34021 taatgaccat cctagaatga gtgcacttaa cacttcatat gcacttctgt cactactcat
    34081 ccaaaataga aatgccacga taatagctaa tacacgtccc atgacaatcc agccataagc
    34141 gtctttagca gatgcttgtt ttggaatatt gaatcgcttc acgatagaac ctgattgtaa
    34201 aaataatgtt gctttaaaaa taccgtgcaa tattaaatga ataatcgctg ctgaatatac
    34261 acccaatgca cattgaacta acataaagcc catttgactc atcgtagagc ccactaattg
    34321 gcgtttatag tcaacttgaa ctaagctaat acccgatcct aacaatacag aaatactaga
    34381 aaggataagt aataatgata acgcaaatcc attatcaaat atcggcgcaa aacgagttag
    34441 aataacacca cctgcattca caattcctgc atgcataatt gccgatactg gcgttggtgc
    34501 cgttacagat tcaatcaacc atcgatgaaa aggaaattgt gctgccggta tcatgacagc
    34561 taatacaagt agtacattcg tcaacaatga ccatgtcgga tgaactatat gttgtggtac
    34621 ccgccactcg ccagtcgcaa tataaatagt tacaattgct ccaacgaatg caagccaacc
    34681 acataaaaat gtcatgcttg ataatttcgc agactcacgt ggcactttcc aaaaacgatt
    34741 aacgttcatc agcaatgtta aacataataa tgtaataccc cagcagagtg ccatcagtct
    34801 taagtcttca gacatccatg ctaaagatgc aaacgacgta atcgcagtga acaatggaaa
    34861 gtaatgtcta taatgatgat cacctagtaa atatcgcatt gaaaactttt gaataataaa
    34921 gccaagcgcc attacaaagc cagctaataa ccaagataaa cgatctattt taaatggacc
    34981 taagacatgt tgaccatgaa taccgaaaaa gccaatgact gcaaataata ctggcatgac
    35041 tagtatgtat aaatgtaatt taatatatct cattggcata actggtgcta aaaacaacaa
    35101 gccacttatc aatgcaatga taagcgcaat aacaaacagt gaaaatagca attgaaaact
    35161 taacactgca taacctcctt atttctaatc tctcgcataa ttgcttatgt ataaaaataa
    35221 aaacctacaa tagtagattc tgtacataat ggcagaaaat ttactattgc aggtttcagt
    35281 ttaactagac actgcatcac ggtacgttga tataccttgt tgcagtgttc tctttaagcg
    35341 tgctcccatg cacatatgta tataaaatgt tacttctgtc tgttcaattc atcttcataa
    35401 atatgctttg cctagacgag acctaacgtg ttattcgttt taaacttata acataaaata
    35461 taattaaatt tctgcttcat gtcaaattca tgagcttaac ctctattaaa ccaatgattg
    35521 taaagatttt gtaaatgcac ctgtacagtt aggcagtatt tcccgtcctt ttaaaataaa
    35581 aaattcgcag ttatgatcat aacaattcaa gttaggaaaa aaatcaatta cgcacaagat
    35641 aactatgtac aatgaagtta actcataagc aaaggaggta atcttaatgg gtatcatcgc
    35701 tggcatcatt aaagttatca aaagcttaat cgaacaattc actggtaaat aagatttcat
    35761 aacaaacaaa ggaggtcttt cacatgggta tcattgcagg aatcattaaa ttcattaaag
    35821 gattaattga gaaattcact ggtaagtaag ttataaaaat ctcatagata tgaacatctt
    35881 atttgaaggg ggccattcac atggaattcg tagcaaaatt attcaaattc tttaaagatt
    35941 tacttggtaa atttttaggt aacaactaat ctcaaacatt aacgatcaac aactcatcac
    36001 tatgttaaat caacatacag gaggacaaaa cgatggctat tgtaggtact atcattaaaa
    36061 tcatcaaagc aattatcgac attttcgcaa aataatttaa gcgaattgaa tacttaaaat
    36121 tctcaggcca ctataccaat agggatggtg gctttgcttt gtgttgattt attgatgtga
    36181 ggtgagtctt gttagtttgt tgcaaataaa tggtcttggt gttttttgta taggacgttc
    36241 ttagtgggac atacggaata ttcgtgatct ttgtagtctg acgcgttata tttttgtggc
    36301 gtgttttatg tttgatactc gagttctgag acattcatga tttggcatgc gaaatcttaa
    36361 tgattttcat gatctagcgc aagatatatt ggccacgtgc ggaattgcgt tgcacgttta
    36421 gactgaaaca ctcgtgtgac cgtaagtgtt aatagtacat tgatagctgc atttacttca
    36481 ctcattttta tgactgttaa acaatgattg taccttcaat taacagttgg tacgatggtt
    36541 ttgccatttt tcatcaacgt aaatataaaa aggactaaga cacatacatg tcctagccct
    36601 atggataaaa tgcaaatttc tgctttatca aaactatcac actttagata gattgaaaac
    36661 aaaaagatcc taagaacacc ttaacttttt attaattgtc ataaattgca aacaattaag
    36721 ccacaattca aaaatgatta tacttcattc aacttatcgt gctggtctaa tttgccattg
    36781 atatggatct tcaaattgtt gccaatctgc atcaatttct tgcgcattga ctaagcatgc
    36841 gtcgagttct tttgttaatt tttcttcatc taattctgta ccaataatga caaattgtgt
    36901 atgacgatcg ccatattctg gatcccattc agctgcgaca tcttgacgtt ctgctaatat
    36961 ttgtgtttgt tgcgcttcag acatactagc cacccaatat gtaactggat gaatattgca
    37021 agatgaccct gcttgagata ataaacatgc tacgtgattg tattgtgcta gccatacgat
    37081 accttttgat cgaacgacat tatttggcat gctttctaac caatcattga accttttagc
    37141 atggaaaggt agacgacgtt tatatacaaa cgatgatata ccatattctt ctgtttcagg
    37201 tgtatgcgat gcatgcccac cagactcaag ttctttgatc catcctgctg actcgctcgc
    37261 tttttcaaaa tcaaaacgct gcgtattcaa gacttctttt aaatctactt cagaatttgt
    37321 tgtcttaata attttagcag tcggttgcaa tgcgcttaac attttttcta acttcgctag
    37381 ttcttcttca ctaattaaat caattttatt aataatcaat acatcacaaa attcaacttg
    37441 gtcaattaat aaatcagcaa tcgaacgctc atctgtttcg tcaacgcttt gatcacgatc
    37501 catcaataaa tcttctgagt tgatgtcatg tacgaagcgg ttagcatcca caactgtaac
    37561 cattgtatct aaacggcaaa tcgctgtaag atcaatgcca agttcatcat caatatatga
    37621 gaaagtttgt gcaacaggta ctggctctga aatccctgtt gactcaataa caatttgatc
    37681 gatgccacct tttttcacta aacgctcaac ttcttttaat aaatcgtctc taagtgtaca
    37741 acagatacaa ccattagaaa gttcgactaa tttttcatct gtacgcgata gtcccccacc
    37801 atctgcgaca agatctttat cgatatttac ttcactcata tcatttacaa ttaccgcgat
    37861 acgtcgacct tctcgatttt gtaaaatatg atttaacaac gttgtcttcc ccgagcctaa
    37921 ataaccactt aataccgtaa ctggaatttt agccatatat ataccctcac ttcaatttat
    37981 ttgtaaatag gaataattac gttttacatt atataggagc gtttcctctt tcgcaatctt
    38041 cgataataaa aaaatagtat acttaattaa attattgagc gctttacttt ataatggaga
    38101 caaagatata tctcacgaaa gagaatcgag gtgtataaac atgttatttg tcattttagt
    38161 tttatatgtt actggtattg catttattct actcagtgtt tttggttcaa agactgaagg
    38221 attatctacg aaacatactt tatataccat tggcagtgct attataacga ttgctatttt
    38281 catttcaatt ggctatgcca ttcaatactt aactgcagcg ctttatggtt tgtaaggtga
    38341 aggtgatgag taacgggtag ttcgggagag gttaacttgc gttgattttg ataaagtgat
    38401 catagctttt agtacttgag gatttttatt gttgctgtta cgaatgtggt catgtttaat
    38461 gcgggacagt aatttaagtt gtttttttac aattgagagt gtgatatttc gattcggttc
    38521 gaattacttt acatgggaat aatataaatt aaaaagaagc ggcctagtgt cagttgtgaa
    38581 tatactgaac attggtcgct ttatttagta gtatgatatg tagtttagct attaattttt
    38641 ttcaggtcat ccttaatgct gtctatctca gacatggcac ttttaaccca atctccttga
    38701 gctgcacctt taaaattagc tttaaaagct tcgcaatgtt gcgccatttg ttcaattaat
    38761 actttttctt caccttttaa tccgttttca atatctttgt atttatgctt atgttcaggt
    38821 gcaataactg tgcgaatatt ttctttttgc gcttccattt tagatatgag attaagtgtt
    38881 tctactgtag tacttatatc tggcattctt aaggtcatat ctggttctat tagagtcatt
    38941 taatctcctc caaattatca gtcacttagc ttatctaact gcttttcata agactttttt
    39001 aagtcttctt tatattcttc taatttccat tcttgctttc taattatctc attgatttct
    39061 tgctcatacg cttctatatt tctcgtcatt ttcctttggg catcttcact ataattcatt
    39121 tcgtgcttca aatattgaaa aacatcataa ctacgctcat tttcttttcg agttctatgc
    39181 ctaagttcat ttaaattttg cagttcattc tctatacgtt ttttctcaga ttcatatgat
    39241 tcttctaatt catttttatt attaatttga ttaaacatca tagtaatcca cctatttgtg
    39301 atgcaagcac ttgatcttta tcaacgattt cattaatact gagtctaact ctcataatat
    39361 aatttgatac agaagaccct atcgttgaaa gttgtctttc ttttgcagat atcatttgtt
    39421 caggttctct taccatacta ctttcattca cttgaccttc atctaaagca ttgagaactc
    39481 catttacact caaatcttca cctacagctt ttccagcttt ttgagcgtct tcccaatttt
    39541 ttccaaattt ctttttcttt tcattataca tttttttgat ttgattaatt tcatcgtcta
    39601 tcagttgact gaatgactgc gcaattgtta aagctgttaa actttctaaa actttttgct
    39661 gcgaagaaga taatcctcct ccatttgtag tcatcatatt tgctctaagt aattctatac
    39721 tagctaattt agagatagca ttatttgaat tctttacaaa tgacttatta ttttcttctg
    39781 cataaccttg tagtttttta agttcttttt ttatagcttt ttgttcttct tcggtcagaa
    39841 agccttccat ttcatgacct ttaccatttt taaagacttt atcattaccg aaaaaaacat
    39901 aatgcttata tttcttcaga ttttctgtca atgcatcatt ttctgcaaca aacctagtaa
    39961 tattaccttt ataattttta tataattcta ataaacgttt tccatctggg gaattaggat
    40021 tcaaactagt tatagaaata ggagctggat tataaaccac aatgttcgga ataccattac
    40081 tcattcctag aactaccgca tctcttccac ctagtgaatg tccagtaata aaatcaatgt
    40141 catacttact ttttatttta tttataaatt cttgtgtatt cgcatatcta tagttttgat
    40201 cagatgcagg agaatataat attttaagat ctgcatatcc atctttaact gtttccaaag
    40261 cattggtaac attttgtctt gaaaattcac cttcttttaa ctttttaaag gcttcgtctt
    40321 gtaaattagt cccagtcatc ccgagagtta ctttgcctgt atctttattt aaaaaagcag
    40381 aagttgttac tccagtaaga gaatcagtgt aactatcaac atattcaaga ttagaaggga
    40441 attttttcat ttttttcaaa tcattaaatt gattaatcac gtctcttttt gatagaccat
    40501 tattattagc attctcaact tcgtatgacg ctttcgaaat agcacttgta gactcagtat
    40561 tttgattaat tgcttttaaa tctattttat acttcctact caacattctc accttcagac
    40621 ggtccaaaat tcaaataatc tcttaatgtt gtctcttgac ctttatcttt tgaaaattta
    40681 tattctattt ttttatatcc tactgagctt ccttttatat caccatctat atataatttt
    40741 agatttggag agttattagt tgtaattgga taaatttctc taagtttttt tacattttta
    40801 tcactattgt tcattttata ttgtgcttca tag
//
LOCUS       DAMQYT010000008        37505 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMQYT010000008 DAMQYT010000000
VERSION     DAMQYT010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 37505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37505
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.008"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            321..1622
                     /locus_tag="PGA33_000311"
     CDS             321..1622
                     /locus_tag="PGA33_000311"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDG6472150.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIGGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
     gene            1636..1986
                     /locus_tag="PGA33_000312"
     CDS             1636..1986
                     /locus_tag="PGA33_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472151.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(2138..2608)
                     /locus_tag="PGA33_000313"
     CDS             complement(2138..2608)
                     /locus_tag="PGA33_000313"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDG6472152.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            2919..4103
                     /locus_tag="PGA33_000314"
     CDS             2919..4103
                     /locus_tag="PGA33_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDG6472153.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGR
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            4103..5296
                     /locus_tag="PGA33_000315"
     CDS             4103..5296
                     /locus_tag="PGA33_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDG6472154.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            5695..6366
                     /locus_tag="PGA33_000316"
     CDS             5695..6366
                     /locus_tag="PGA33_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HDG6472155.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(6496..7299)
                     /gene="coaW"
                     /locus_tag="PGA33_000317"
     CDS             complement(6496..7299)
                     /gene="coaW"
                     /locus_tag="PGA33_000317"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HDG6472156.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLEK"
     gene            7723..8583
                     /locus_tag="PGA33_000318"
     CDS             7723..8583
                     /locus_tag="PGA33_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6472157.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            8695..9225
                     /locus_tag="PGA33_000319"
     CDS             8695..9225
                     /locus_tag="PGA33_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDG6472158.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            9561..11171
                     /locus_tag="PGA33_000320"
     CDS             9561..11171
                     /locus_tag="PGA33_000320"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDG6472159.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(11280..11801)
                     /locus_tag="PGA33_000321"
     CDS             complement(11280..11801)
                     /locus_tag="PGA33_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HDG6472160.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            12019..12879
                     /locus_tag="PGA33_000322"
     CDS             12019..12879
                     /locus_tag="PGA33_000322"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDG6472161.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            13345..14604
                     /locus_tag="PGA33_000323"
     CDS             13345..14604
                     /locus_tag="PGA33_000323"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDG6472162.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            14693..15028
                     /locus_tag="PGA33_000324"
     CDS             14693..15028
                     /locus_tag="PGA33_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDG6472163.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            15266..16693
                     /locus_tag="PGA33_000325"
     CDS             15266..16693
                     /locus_tag="PGA33_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDG6472164.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
     gene            16940..18256
                     /gene="rho"
                     /locus_tag="PGA33_000326"
     CDS             16940..18256
                     /gene="rho"
                     /locus_tag="PGA33_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDG6472165.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            18374..18628
                     /locus_tag="PGA33_000327"
     CDS             18374..18628
                     /locus_tag="PGA33_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDG6472166.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            18975..19574
                     /locus_tag="PGA33_000328"
     CDS             18975..19574
                     /locus_tag="PGA33_000328"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDG6472167.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            19575..20651
                     /gene="prfA"
                     /locus_tag="PGA33_000329"
     CDS             19575..20651
                     /gene="prfA"
                     /locus_tag="PGA33_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDG6472168.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            20638..21474
                     /gene="prmC"
                     /locus_tag="PGA33_000330"
     CDS             20638..21474
                     /gene="prmC"
                     /locus_tag="PGA33_000330"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDG6472169.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            21558..22604
                     /locus_tag="PGA33_000331"
     CDS             21558..22604
                     /locus_tag="PGA33_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDG6472170.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKLGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            22601..23020
                     /locus_tag="PGA33_000332"
     CDS             22601..23020
                     /locus_tag="PGA33_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDG6472171.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            23127..23651
                     /locus_tag="PGA33_000333"
     CDS             23127..23651
                     /locus_tag="PGA33_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDG6472172.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            23678..24916
                     /locus_tag="PGA33_000334"
     CDS             23678..24916
                     /locus_tag="PGA33_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDG6472173.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALGMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            24944..25573
                     /gene="upp"
                     /locus_tag="PGA33_000335"
     CDS             24944..25573
                     /gene="upp"
                     /locus_tag="PGA33_000335"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDG6472174.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            25597..26724
                     /gene="wecB"
                     /locus_tag="PGA33_000336"
     CDS             25597..26724
                     /gene="wecB"
                     /locus_tag="PGA33_000336"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDG6472175.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            26792..27244
                     /locus_tag="PGA33_000337"
     CDS             26792..27244
                     /locus_tag="PGA33_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDG6472176.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYFYLIIILGGLYLYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            27265..27993
                     /gene="atpB"
                     /locus_tag="PGA33_000338"
     CDS             27265..27993
                     /gene="atpB"
                     /locus_tag="PGA33_000338"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDG6472177.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            28036..28248
                     /gene="atpE"
                     /locus_tag="PGA33_000339"
     CDS             28036..28248
                     /gene="atpE"
                     /locus_tag="PGA33_000339"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDG6472178.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            28446..28967
                     /locus_tag="PGA33_000340"
     CDS             28446..28967
                     /locus_tag="PGA33_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDG6472179.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            28967..29506
                     /locus_tag="PGA33_000341"
     CDS             28967..29506
                     /locus_tag="PGA33_000341"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDG6472180.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLR
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            29528..31036
                     /locus_tag="PGA33_000342"
     CDS             29528..31036
                     /locus_tag="PGA33_000342"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDG6472181.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            31067..31933
                     /locus_tag="PGA33_000343"
     CDS             31067..31933
                     /locus_tag="PGA33_000343"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDG6472182.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            31955..33367
                     /gene="atpD"
                     /locus_tag="PGA33_000344"
     CDS             31955..33367
                     /gene="atpD"
                     /locus_tag="PGA33_000344"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDG6472183.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            33387..33791
                     /locus_tag="PGA33_000345"
     CDS             33387..33791
                     /locus_tag="PGA33_000345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDG6472184.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            34129..34221
                     /locus_tag="PGA33_000346"
     CDS             34129..34221
                     /locus_tag="PGA33_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472185.1"
                     /translation="MLKSKFPSPLSTVDISFFEIIYIFSYQDIL"
     gene            34353..34586
                     /locus_tag="PGA33_000347"
     CDS             34353..34586
                     /locus_tag="PGA33_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDG6472186.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            34697..35962
                     /gene="murA"
                     /locus_tag="PGA33_000348"
     CDS             34697..35962
                     /gene="murA"
                     /locus_tag="PGA33_000348"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDG6472187.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRDAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            35996..36436
                     /gene="fabZ"
                     /locus_tag="PGA33_000349"
     CDS             35996..36436
                     /gene="fabZ"
                     /locus_tag="PGA33_000349"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDG6472188.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(36495..36935)
                     /locus_tag="PGA33_000350"
     CDS             complement(36495..36935)
                     /locus_tag="PGA33_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HDG6472189.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            37129..37476
                     /locus_tag="PGA33_000351"
     CDS             37129..37476
                     /locus_tag="PGA33_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDG6472190.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSI"
BASE COUNT        13303 a         5381 c         7351 g        11470 t
ORIGIN      
        1 gttcaaatta tgcaaggttt agaagcagat tcagattact aattttataa aaatatgtaa
       61 gcatgggaca tcgtaaccat atataagtat gtcgatatca gctttaagac atcataaatg
      121 ccatgatgat tctaagataa tttaataggg actttaacgt tataaccaat ctatttaaga
      181 ataataacat cgcattgaaa ttgtgatatt aaaaatgaaa tcgatagctt gctataataa
      241 ttttcgcttg tgcttttaaa aatggactag agaaagcgca gatgctttgt cctaagcttt
      301 ttaagaaaag gatgataaaa atgagaatga tagacattat tgagaaaaag cgtgacggtc
      361 atacactaac aacggaagaa attaatttct ttattggcgg ctatgttaaa ggggatattc
      421 ctgattacca agcatcaagt ttagcaatgg cgatttattt ccaagatatg aatgatgatg
      481 agcgtgcagc attaacgatg gctatggtta attctggtga tatgatagat ttgagtgata
      541 ttaaaggtgt caaagtagat aagcactcaa caggtggtgt aggagataca actacattgg
      601 ttttagcacc attagtagca gctgtagatg ttcctgttgc aaaaatgagt gggcgtggat
      661 taggtcatac aggtggtacg attgataaat tagaagcaat tgatggtttt catgttgaaa
      721 tagatgaagc aacatttgtg aaattggtta atgaaaataa agtggcagtt gtaggacaat
      781 caggaaattt aactcctgca gacaaaaaat tatatgcctt aagggatgtt actggtactg
      841 tcaattcaat accattaatt gcctcttcaa ttatgagtaa aaagattgct gctggtgcag
      901 atgcaattgt attagatgta aaaactggta gcggtgcatt tatgaaaaca ttagaagatg
      961 ctgaagcatt agcgcacgca atggtacgta ttggtaataa tgtgggacgt aatacgatgg
     1021 cgattatttc tgatatgaat cagccacttg gacgtgcgat tggcaatgcg cttgagttac
     1081 aagaagcaat tgatacgttg aaaggacaag gtccgaaaga tttaacagaa cttgtattaa
     1141 cattaggttc tcaaatggtt gtacttgcaa acaaagctga aacgcttgaa gaagcgagag
     1201 cgttgctaat tgaggcaatt aattcaggtg cagcattaga aaaattcaaa acatttatta
     1261 aaaaccaagg tggcgacgaa actgttattg accatccaga gcgtttgcca caagctcaat
     1321 atcaaattga atataaagct aaaaaatcag gttatgtgac tgaattagtc tctaacgata
     1381 taggtgtcgc ttcgatgatg ttaggagcgg gacgtttaac aaaagaggat gatattgatt
     1441 tagcggttgg tattgtttta aataaaaaaa ttggtgataa agtagaagaa ggagaatcat
     1501 tattaacgat tcacagtaat cgccaagatg tagacgatgt tgttaaaaaa ttagattcaa
     1561 gcataacaat tgctgatcat gttgtatcgc ctacattaat tcataaaatc attacagaat
     1621 aggagttgaa gctgattgaa catactaaga tatttattag gaataggttt tagtgtaata
     1681 ggtgtattgc attttacacg agaacgacaa tttagaaata tcataccgaa atgtttgcca
     1741 cttcgaaaaa cagcggttct tgttacagga atctttgaga tattatttgg acttgcactt
     1801 ttaattaaaa gaccatctca atgcttgaaa aatacaatca atctattctt gctagctgta
     1861 ttcccagcta atatctatat ggcagttaaa aagttacctt taggagataa acaattgcca
     1921 acatggcaac tatatttaag gttacctatg cagttcgtgt taatgggact aattaagaaa
     1981 ttatagtggt tctcaaataa atcggacaaa tgttaaagat tttacttttt catttgttcg
     2041 attttttgac tttgaagcat aataaaaagc agttggacaa tggcgattgt aaaatcattg
     2101 ttcgactgct tttctgatta acttgattga ctaagattta ttttcctgta ccgaaaacat
     2161 catgccattc gtttcgtttg tctagaaatg cttgagcgat agttttagca ccttctaatg
     2221 aatgacttgc tgcccagcca cattgtactt cattacaagc aggcacttca gtagcattta
     2281 gcacatcatt taaagttgct tcaacaatat ttaatacatc atcataatta tcatgattaa
     2341 taaatgatac atagaaacca gtttggcaac ccataggact taaatcaaca actttgtcac
     2401 tatgatttct aatattttca gccattaaat gttctaatga atgtagtccg ggcatatcca
     2461 tatgttcttt gtttggttgt ttgaaacgaa tgtcgtattt gtgaatgaca tctccgttta
     2521 atccttccat cgttcccgct aatctaataa atggggcaac caccttagta tgatctaaat
     2581 tgaaactttc aacattcatt tttgtcattt tgaatttcct cctattaact actcaaaact
     2641 ataaaacgct tatgatttgt tgcattataa aacttaagtt tgttgtaagt tgtgtttcta
     2701 acaatactta aattgtaaca atagggctta tgttttacaa tttgaaactg ttttataaat
     2761 ttatgaaagg cttttagtta ttagaaaatt ttgtaaataa agaaaaacta gatgacatca
     2821 tgagataaaa ccgatagaat aagtaataat tcattttgaa agaacaaaat attgtggatg
     2881 attaaaatga gtgtagggta aaagggagat agtaaaaaat gagtgaaaaa caacaaattc
     2941 tcgattatat agaaacaaat aaatatagtt atatcgaaat cagtcataga attcatgaac
     3001 gtcctgaact tggtaatgaa gaaatatttg cgtctcgaac tttaatagat cgtttgaaag
     3061 agcatgattt tgaaattgaa accgagattg ctgggcatgc aactgggttt atagcgacat
     3121 atgattcagg acttgacggg ccagctatag gttttttagc agaatacgat gctttgccag
     3181 gattgggtca tgcttgtggt cataatatca ttggaactgc aagtgttctt ggtgcaattg
     3241 gtttgaagca agtgattgac caaattggtg gtaaagtagt cgttcttgga tgtccagctg
     3301 aagaaggtgg ggaaaatggt agcgctaaag cttcttatgt caaggctggt gtgattgatc
     3361 aaatagacat tgccttaatg attcatccgg gaaatgaaac ttataaaacg attgatactt
     3421 tggcagtgga tgttttagat gttaaatttt acggaaaaag tgctcatgcc tctgaaaatg
     3481 cagatgaagc gttaaatgca ttagacgcta tgattagtta ttttaatggt gtagcacaac
     3541 tacgacaaca tattaaaaaa gatcaacgtg tgcatggtgt gattttagat ggcgggaaag
     3601 cagctaatat tattccagac tatacacatg ctcgttttta tactagagca atgacgcgta
     3661 aagaattgga tatattaaca gaaaaagtaa atcaaatcgc acgtggagct gcgatacaga
     3721 ctggttgtga ttatgaattt ggtcgaattc aaaacggtgt gaatgaattc attaaaacgc
     3781 cgaaattaga tgatttattt gctaaatatg ctgaagaagt tggtgaagca gttattgatg
     3841 atgattttgg ttatggctct acggatacag ggaacgtaag tcatgttgtg ccaacaatac
     3901 atcctcatat taaaatagga tcacgtaatt tagtaggaca tacgcataga tttagagaag
     3961 cggctgcgag tgtacatggt gatgaagcat taattaaagg cgctaaaata atggcgttga
     4021 tggggttaga gttaattaca aatcaagacg tttatcaaga cattattgaa gagcatgcgc
     4081 atttgaaagg gaatgggaag taatgacaaa taatggtaat gagccgaact taacgttaag
     4141 tgatttatat gacaaagatg ttgtctatac gtcaagacct tcgtatatat cgaatccatg
     4201 gttgaaaccg gacgaacatc aatctaattt tttaactggt agggagttat taatagcaaa
     4261 tcaattacct gttattgttc acgaagctag tgcgacagac aaattgcatc agctatttca
     4321 agttattggt aaggaagtac cgaactcaat ttatacattt aacaatcagc aatcttacga
     4381 aaacttgatc aaacaattgg cacataagga aaataaaaag atttattttc aatatattca
     4441 tgatgaaaca atcttgaatc agcaatatta tgcattagat aaaacgttat ttgttgcttt
     4501 aaataataaa gcaagaatac ctgaatggac aaatgggaag tttttaccaa aaagaaaagt
     4561 tgtaaaaatt gagcaattcg aaaacgagat taagaattgg gaattcccgt tagtaattaa
     4621 gccgggagat gatcttccga cagcaggtgg ctatggcgtt atgatttgtt atcatgatgc
     4681 agatttacaa aaagcgataa cgcgtattaa agaggcaact gctgaaacga attcgcttat
     4741 tatcgaacag aagattgaag aaaaagcgaa ttattgcgtt cagtttgcat actcggaatc
     4801 gctaggcatt caatatcttg gtgcggcaac gcaattaacg gataagtacg gattttataa
     4861 cggaaatgaa aatactacta atgtaccaga gcacgttata gaagcaggta gacaaattat
     4921 ggaaaacggt gtgaatcaag gattctttgg tgtagctggt tttgacctac tcgtcgatga
     4981 ggatgataac gtttatgcga ttgatttaaa ctttagacaa aatggttcaa cgagcatgtt
     5041 attacttgct aacgagttga attcaggata tcaaaagttt tatagttatc attcaaaagg
     5101 tgataacaca catttcttca atacgatttt gaaatatgtc aaagaaggta gtttataccc
     5161 gttatcttat tatgatggtg attggtacgg tgaagataaa gttaaatcaa ggtttggctg
     5221 tatttggcat ggtgattcaa aagaaacagt actggagaat gaacgcgcat ttttagctga
     5281 acttgaacac tattagagtt cggaacataa ggcgctacaa tgttgtgttg ccagtagttg
     5341 actgaatatg cgtttgtaac aagctttttt cgattctagt caacagtaat taaatttatg
     5401 atatggcaat actttgtaat actaatatta aatggcgact tttatttcac tatgttataa
     5461 gagttgccat tttgttgata aaggtatact aaaggttatc gttttgaaat ttttagtaac
     5521 tagatatgtt tcgtgttata gaccgaattt gtgtatacgt aaaatttaat gctattgaat
     5581 ttttaaaatg aaaaacatga cattaaattg aattcataat atgtctaatt gactaacttg
     5641 ttggagtcat ttactatttt atgtatgaca tattttaaaa agtgagggtc aagcatgtct
     5701 tataaagcat atccattctt tagagatata ttaataaatg aatgtattta tttcgcctct
     5761 aaaaataaaa aactagtacg cctaaattat aaaagtgaag cgtatgtagg cgtttggaca
     5821 gaagaaagtg tggccgtatc atttttaaca agtcgtgata ttccatttga taaagttgta
     5881 aaaatggacg ttgatcgttt tgctacttat gaattagatg aattgtttga tgaacaagac
     5941 catattatta tgaatcaaac aatggaagaa gaagggcatc tactaaacgt tgtagctgtt
     6001 acacaagaag tgatgacgga attagataaa attagaatca aagaatttgt ccaagatgta
     6061 gcgaaatatg atgaagtata cggcttaact aaaaaaggta gtaagcagtt tattctcatt
     6121 agtgaaaatg atagcgacga aaaaaagccg catattatgc ctgtatggag tattaaaaac
     6181 agagcgttaa aagttcgaga tgaagatttt gaagagtgtg atttaattac gattgaaggt
     6241 tctgttttcg gagaatggct agatgaactt agagatgatc ataaagccgt tgcgatagat
     6301 ttaaaaactg gcgtggttgg tacaattgtt tcagcgcaaa aattgtctaa tgaacttaca
     6361 ttttaattga attgagttgt aagtaattaa gtgataataa atggaacaat acgtattcaa
     6421 aacacttaga ccataaaata aaaggccatt tatatagcgt ttatttaaaa caacgcgcat
     6481 ataaatggtc tttttctatt tttctaaata taatgcacca atagcacctg aaaatgcgcc
     6541 gttttcaaca tagtacggtt tgcaaccgcg taacacagta taatcttcca caactttgcg
     6601 taataaagcg ttattatgaa atgaagaacc gatataaacg atattttcag ttttaaattc
     6661 acgtgcaaca gtaatggcca ttgtcgtaac aacttcgcca acgacaccaa taacggctgc
     6721 taatttattg ctaggtgtaa aatcagcatc taaatgatgt agtacatgac caaaattagc
     6781 tgctgttaaa tcaccgggaa tgggtggttc ggtatcttta taaatatgtc taacctttaa
     6841 atcgatagtg ttacgatcac cgtgttgtgc catgtcagtt aactgtttat aatcagtgat
     6901 ttgacttagt aaataaccga gtccttgaat catgcctcca cctgtaccga taccgcctac
     6961 acgacgttgt gattggccgt cgaaataatg tagtgacgta ccggtaccaa catttgcaaa
     7021 aatataatct gctaagtcat ggccttgctc ttttaacaaa atacctagtc cttgagatgc
     7081 agcatcaaac tctacaaaaa tttgtgcagg aatgttgatg ttttcagcaa tgacacctgc
     7141 attacctcca gttaagcata atttttcaat ttgctgttgg tttaaccatt ccacaacttg
     7201 atcaatattt ttagttaatt cagttttaaa agtacgttgg ttatcttgct cttgaacgat
     7261 tttaattagt gtaccgccag cgtcaatgcc aactttcata agattcccac ctcattatta
     7321 atgtctatcc ttaaataata gtatagtaaa atgactaaaa aacaagtaat aatagtaatt
     7381 attaacaaat ttgatgccat tgcatttcaa cattgtaagc gtatcgcaat taatgtttta
     7441 caaacgtgga cgttaagtta tatatattat tttctaggaa ttttgaagtt gtataggatt
     7501 gttagttagt gacgcaatat taaaagtagt tcgtacgcag tgtatttgta agtctctgat
     7561 taaaatgata agtaatgagg aatagtacat taattttgaa atttaaaaaa tataaataag
     7621 taatttattt aacttagagc aaataatggt atcgtagtga aataataggt aaaataatat
     7681 ggggattcat gcttcatata taaaaagata ggggttaaat atatggctaa agaactttgt
     7741 tttgaaggta tcactttaaa agcatttgat gaacaatatc gttcagcaat taatgatttt
     7801 gacttgaatg aaagacaaca aatatattca tctttaccta aagaagttat tgatgatgca
     7861 attaatgatg ctgataggat tgctaacgta gcaataaacg ataaaaatga agtggtgggc
     7921 ttttttgtat tacatcgtta ctatcagcat gaaggttatg atacacctga aaatgtcgtt
     7981 tatattcgtt cattatcgat taatgaaaaa tatcaaggtt ttggatatgg cacgaaaata
     8041 atgatgtcat tgccgcaata tgttcaaggt gtatttcctg attttaatca tctatatcta
     8101 gtagtagatg cggaaaatga caatgcttgg aacctatacg aacgtgctgg attcatgcat
     8161 acagcgacta aagaagaagg accaattggc aaagaacgac tatattactt ggacttagat
     8221 tcaaaacatg tttcatcatt aaagcttgaa gaagaaagtc gttcagaagt gaccaatgta
     8281 catatcatta atttaatgat tgatggccaa aaggttggct ttatcgcatt ggagcagatt
     8341 ggtgaacgca tgaacattgc tgctattgaa gtggataaat catatcgctt taatggtatt
     8401 ggttcaagtg ctctgcgaca attgccaact tacttaagaa aaaactatga caaccttaat
     8461 gtgattacga tgattctgtt tggagagaat aatgatttta aaccattatg tttaaatagt
     8521 aatttcgttg aaatcgaaca aactgatgat tatgtcgttt tcgaaaaata tttaaattac
     8581 taacagtgat tgcgaaatat gatattgtca tttataattt agttttgtta ctatatataa
     8641 atgaattcag acgtataaat ttagattata tccttcgaaa ggaagtattg ggcaatgaaa
     8701 attcaagatt atacaaaaca aatggttgat gaaaaatcat ttattgatat ggcttataca
     8761 ttattgaatg ataaaggcga aacaatgaac ttatatgata tcatcgatga atttagagcg
     8821 ttaggtgatt atgagtacga agaaattgaa aatcgtgttg tacaatttta cacggattta
     8881 aacacagatg gtcgtttttt aaatgttgga gaaaatttat ggggattacg tgattggtat
     8941 tcggtagatg atattgaaga gaaaatcgca ccaactattc aaaaattcga tattctggat
     9001 gcagatgatg aagaagatca aaacttaaaa ttattgggcg aagatgaaat ggatgacgac
     9061 gatgatattc cagctcaaac agatgatcaa gaagaactaa atgatccaga agatgagcag
     9121 gttgaagaag aaatcaatca ttcggatata gtcattgaag aagatgaaga tgaactagac
     9181 gaagacgaag aagtgtttga agacgaagaa gacttcaacg attaattttt tgtttgactt
     9241 ttagttgaaa gatgataaaa ttttattcgg gctcctttaa ataggacacg tgtataaaat
     9301 ttatacgctc cccttacaga atttgtgaga gggagcgttt ttttatttaa ttgagtaaat
     9361 caagaaatga taacgcaaaa atcaaagttg taaatgatat acatagtgac atagcagtat
     9421 ggaaacggta agtaaacaga atttaatttt gtcgaattcg acaaataaac aacttgaatg
     9481 agcttgcttt aatgttatgt tttacgtaat ttttacaatt gatgaggaag cattcccttt
     9541 aataattagg aggtcaagac atgacaaaat ttatttttgt aacaggtggc gtagtttcat
     9601 cattagggaa gggtattaca gcatcttctc taggtagatt attaaaagat agaggtctaa
     9661 atgtaacaat tcaaaaattc gatccatact taaatgttga cccaggtaca atgagtcctt
     9721 atcaacatgg tgaagtattc gtaacggatg atggtgcaga aactgaccta gacttaggac
     9781 attacgaaag atttattgat attaatttaa acaagttttc aaatgtgaca gccggtaaag
     9841 tgtattcaca cgtattgaaa aaagaacgtc gtggtgatta cttaggcgga acagttcaag
     9901 ttattccgca tattacaaat gaaattaaag aacgtttatt acttgcaggg gaaagtacga
     9961 atgcagacgt tgttatcact gaaattggcg gtacaacagg tgatattgag tcattaccgt
    10021 ttattgaagc gattcgtcaa attcgtagcg atttaggtag agaaaatgtt atgtatgttc
    10081 actgtacatt actgccttat attaaagctg ctggagaaat gaaaacgaag ccaacacaac
    10141 atagtgttaa agaattacga ggcttaggta ttcaaccaga cttaatcgtt gtaagaactg
    10201 aatatgaaat gacacaagat ttaaaagata aaattgcatt attctgtgac attaataaag
    10261 aaagtgttat tgaatgtcgt gatgcagact ctttatacga aattccatta caattaagcc
    10321 aacaaaatat ggatgatatc gttattaaac gtttacaatt aaacgcgaaa tatgaaacac
    10381 agcttgatga atggaaacag ttgttagata tcgttaataa tttagatggt aaaattacaa
    10441 ttggtttagt aggtaaatat gttagcttac aagatgcata tttatcagtt gttgaatcat
    10501 tgaaacatgc tggatatcct tttgccaaag atattgacat tagatggatt gattcaagtg
    10561 aagtaacaga tgaaaatgca gccgaatacc ttgcagatgt cgacggtatt ttagtaccag
    10621 gtggatttgg tttccgtgca agtgaaggta aaattagtgc aattaagtat gctagagaaa
    10681 acaatgtacc attctttggt atttgtttag gaatgcaact tgcaacagtt gaattttcaa
    10741 gaaacgtatt aggccttgaa ggcgcacatt cagctgaatt agacccagca acaccatacc
    10801 caattataga tttattacca gaacaaaaag atatcgaaga tttaggtggt acattacgct
    10861 taggcttata tccatgttca attaaagaag gcacattggc acaagatgtt tatggtaaag
    10921 cggaaattga agaaagacat cgtcatcgtt atgaatttaa taatgactat agagaacaat
    10981 tagaagcaaa tggtatggtg atttctggta caagtccaga tggacgttta gtagaaatgg
    11041 tagagattcc gacaaatgat ttctttattg cttgtcaatt ccacccagaa ttcttatcta
    11101 gaccaaatcg tccgcacccg atttttaaat catttattga agcttcatta aaatatcaac
    11161 aaaataaata aatttgctaa taaaaccggt actttcattg ttaaacattg aaagtaccgg
    11221 tttttcgtat aattttaata ttatgttagt gacaaggtat gaaataacaa tagtgacttt
    11281 tataattcta agtctcttgt catttcaatc atttgtgtat aaatgtcata gtatacataa
    11341 ttcaatgcca tcgcatgtgg ttggacaatc ttatcgtaat cttcagtgta gactataggt
    11401 cttggtgtag ataaatcgat aaaatgtacg agatgatcag ggaaatcatc tgttttaggt
    11461 ttgttgctta ttaagaccac atcgatatct aagtcgataa gtttttgaat atctaatgca
    11521 acttgatcat tataaaatgg tgcgaataat aatacacgat cagttgagtc aatttcttta
    11581 aagtctttaa tagcgtcaag ttttcggcta gattttaaac gctcatcact atgtagaatg
    11641 aaactttcga aaaactgtaa atcatcataa ccttttacat aaacataacc ttcgccacca
    11701 attgcttgaa ttaaacattg ggcggccatt tgaatttcta aagattgttt ttctagccta
    11761 ttaaagatac ctattagttg tgtgtttaag atttttgaca tctttatcct ccaatctact
    11821 tataaaatat tgtaattaat gactacatat tatgcaacgg cttaaattgt ataaaaatgt
    11881 atacgtttgc atttagtata actatcgcat ttttcaaaaa atacacattt aatctgcagt
    11941 atttcaatgc attgacgcta tttttttgat ataattactt tgaaaaatac gtgcgtaagc
    12001 actcaaggag gaactttcat gcctttagtt tcaatgaaag aaatgttaat tgatgcaaaa
    12061 gaaaatggtt atgcggtagg tcaatacaat attaataacc tagaattcac tcaagcaatt
    12121 ttagaagcgt cacaagaaga aaatgcacct gtaattttag gtgtttctga aggtgctgct
    12181 cgttacatga gcggtttcta cacaattgtt aaaatggttg aagggttaat gcatgactta
    12241 aacatcacta ttcctgtagc aatccattta gaccatggtt caagctttga aaaatgtaaa
    12301 gaagctatcg atgctggttt cacatcagta atgatcgatg cttcacacag cccattcgaa
    12361 gaaaacgtag caacaactaa aaaagttgtt gaatacgctc atgaaaaagg tgtttctgta
    12421 gaagctgaat taggtactgt tggtggacaa gaagatgatg ttgtagcaga cggcatcatt
    12481 tatgctgatc ctaaagaatg tcaagaacta gttgaaaaaa ctggtattga tgcattagcg
    12541 ccagcattag gttcagttca tggtccatac aaaggtgaac caaaattagg atttaaagaa
    12601 atggaagaaa tcggtttatc tacaggttta ccattagtat tacacggtgg tactggtatc
    12661 ccgactaaag atatccaaaa agcaattcca tttggtacag ctaaaattaa cgtaaacact
    12721 gaaaaccaaa tcgcttcagc aaaagcagtt cgtgacgttt taaataacga caaagaagtt
    12781 tacgatcctc gtaaatactt aggacctgca cgtgaagcca tcaaagaaac agttaaaggt
    12841 aaaattaaag agttcggtac ttctaaccgc gctaaataat taatatttag tctttaagtt
    12901 attaataacg tagggatatt aattttaaaa gaagcagaca aaatggtgtt tgcttctttt
    12961 ttatgtcgta taagtaataa ataaaacagt ttgattttaa aatgaaagcg taaaaatggt
    13021 aaaatatatc aaaattgatt gtgatataat tataaggaaa atgagcaatt tatgaaaaaa
    13081 gtttacgtga aaaccgtaga attaaaacta aataattgtc aaaacaacgt caatatttag
    13141 ttgaataatc agactttaga ccatggtcaa gtggggaaga acagcatata ttagtaaagg
    13201 tgaatgattt gttattaatc aatcgaaaat agaaagacaa gattttaacg attaaaataa
    13261 actattttac aaataaagta aaattaattt ataatgctaa taatgcaaaa aattaaaaag
    13321 taatggacaa aggagataaa tgatatggct caagaggtaa taaaaataag aggtggacgc
    13381 acactaaatg gggaagttaa tatcagtggc gcgaaaaaca gtgctgtagc tatcattcct
    13441 gcaacattat tagctcaagg acatgtgaaa ttagaagggt taccgcaaat ctctgatgtt
    13501 aaaactttag tgagtttatt agaagattta aatattaagg catcactaaa tggtacggaa
    13561 ttagaagttg atacaactga aatacaaaat gctgcattac caaataataa agttgaatca
    13621 ttacgtgctt catactatat gatgggtgcc atgttaggta gatttaaaaa atgtgtgatt
    13681 ggtttaccgg gaggatgtcc acttgggcca cgtccaattg atcaacatat taaaggattt
    13741 aaagctttag gtgctgaaat tgatgaatca agtacgacat caatgaaaat cgaagctaaa
    13801 gagttaaaag gtgcacatat ttttctagat atggttagcg taggtgcaac aattaacatt
    13861 atgctagctg cagtttatgc aacaggacaa actgtaattg aaaatgctgc taaagaaccg
    13921 gaagtagttg atgttgctaa cttcttaaca agtatgggag ctaatattaa gggagcgggt
    13981 acatcaacaa ttaaaatcaa tggcgtcaaa gaattacatg gttctgagta tcaagttata
    14041 cctgatagaa ttgaagcagg cacatatatg tgtatcgctg cagcatgtgg agaaaatgtt
    14101 atattaaata atattgttcc gaaacatgta gaaacattaa ctgctaaatt tagtgaatta
    14161 ggtgtgaatg ttgatgtaag agacgaaaga attcgcatca ataataatgc gccatatcaa
    14221 tttgttgata ttaaaacact agtatatcca ggttttgcta ctgatttgca acagcctatt
    14281 acaccattat tatttatggc aaatggtcct tcatttgtaa ctgatacgat ttatccggag
    14341 cgttttaaac atgttgaaga attaaagcgc atgggtgcaa atattgaagt tgacgaaggc
    14401 acagcaacga taaaaccatc aacattacat ggtgctgaag tttatgctag cgatttaaga
    14461 gcaggagctt gtttaattat tgctggttta attgctgagg gtgtaactac aatttacaat
    14521 gttaaacata tttatagagg ttatacagat attgtagaac acttaaaagc tttaggtgca
    14581 gatatttgga cggaaactgt atagatttat cttgttttgt ggtataataa ttatactgat
    14641 taaaaaatgg tgttcgttta cattctaaat ttattgatga gaggtgaaga tgatggaagt
    14701 atgtccgtat ctcgaagaaa cttttaaaat acttggtaga agttggaatg gattaatcat
    14761 taattatctc tcaagatgta atgactgttc agcacacttt tccgatatga aaagagattt
    14821 gaaaacaata acaccacgtg ctttaagtct taagttgtca gagcttgcac aatgggagtt
    14881 agttgagaag caaatcattt ctacgagtcc agtacaaatt atttatgtgc taactgaaaa
    14941 aggtaaagcg ttagcagagg ctttacatcc aattgaagca tgggcgcaat catatgtcga
    15001 tttaacagat caacgtactg ctaaataatt acatgacgtt taaacatagc attgattata
    15061 actatttcta agtcttcgca ttatttgcga tgatgtggga atagttattt ttatttaaaa
    15121 atataaaaaa atagatgcag caaaatttta aagcatttta ttttgaacat attaaaaggg
    15181 agcgtatcat aatggaatgt aatgtttata tcgtatgcat tacggataaa taatatataa
    15241 atcattcttg aggagtgaaa gaataatgag agactacaca aagcaataca ttaatggcga
    15301 atgggtagaa agtaatagta atgaaacgat agaagttata aatccagcaa ccgaagaagt
    15361 aatcgggaaa gttgctaaag gtaataaagc tgatgttgat aaagccgtcg aggcggcaga
    15421 cgatgtttat ttagagttcc gtcatacatc tgtgaaagaa agacaagcgt tattagataa
    15481 aattgtaaaa gaatatgaaa acagaaaaga cgatattgta caagctatta cggatgaatt
    15541 aggtgctcct ttatcattat ctgagcgtgt ccattatcaa atgggactaa accattttgt
    15601 tgcagcgaga gacgcattag ataactacga atttgaagaa cgccgcggag atgatttagt
    15661 tgttaaagaa gcaatcggtg tatctggatt aattacaccg tggaacttcc ctacaaacca
    15721 aacatcatta aaattagcag cagcatttgc ggctggtagt ccagttgtac ttaaaccatc
    15781 tgaagaaaca ccatttgcag ctgttatttt agctgagatt tttgataaag tcggtgttcc
    15841 taaaggtgta tttaaccttg ttaatggtga tggtgctggt gttgggaatc ctttatctga
    15901 acatcctaaa gtacgcatga tgtcatttac aggatcaggc cctactggtt ctaaaattat
    15961 ggaaaaagcc gctaaagatt ttaaaaaggt atcattagag cttggtggca aatcaccata
    16021 tatcgtccta gatgacgtag atattaaaga agcggctaaa gcaacaacag gcaaagttgt
    16081 taataatact ggtcaagtat gtacagctgg tacacgtgtt ttagtgccta acaaaattaa
    16141 agatgcattc ttagctgaat taaaagaaca atttagccaa gtgcgtgtcg gtaatccaag
    16201 agaagatggt acacaagtag gccctatcat tagtaaaaaa caatttgatc aagtacaaaa
    16261 ttatattaat aaaggtattg aagaaggtgc tgaattattt tatggtggtc ctggtaaacc
    16321 agaaggactt gaaaaaggat actttgcacg tccgacaatt tttattaatg tagataatca
    16381 aatgacgata gcacaagaag aaatttttgg gccagtaatg tcagttatca cttataacga
    16441 tttagatgaa gcgattcaaa ttgcaaatga tacaaaatat ggtttggcag gatatgttat
    16501 tggtaaggac aaagaaacat tgcataaagt agctcgttct attgaagcag gtacagtaga
    16561 aataaacgaa gcaggtagaa agccagattt accatttggt ggctataaac aatctggttt
    16621 aggtcgtgaa tggggcgatt atggtattga agagttctta gaagtgaaat ctatagctgg
    16681 atattttaaa taaatttgat ttttgaatta aaaatcgcaa taaaacagtg cacatgacta
    16741 attaagtttt gtgtactgtt ttaattttgc aatttttata aatagatttt gtaattaaaa
    16801 taaaaatttg ctatagttat tcatgtattt aaaaggttgg ggattagcat aatgggattg
    16861 tgctagcaca gttatttatg cattgtcatg cctatctatt acttactaac taaaaaataa
    16921 tgaaatgggt gtaaactata tgcctgaaag agaacgtaca tctcctcagt atgaatcatt
    16981 ccacgaattg tacaagaact atactaccaa ggaactcact caaaaagcta aaactcttaa
    17041 gttgacgaac tatagtaaat taaataaaaa agaacttgtt ctagctatta tggaagcaca
    17101 aatggaaaaa gatggtaact attatatgga aggtatctta gatgatatac aaccagatgg
    17161 ttatggtttt ttaagaacag tgaactattc taaaggggaa aaagatattt atatatctgc
    17221 tagccaaatt cgtcgttttg aaattaaacg tggggataaa gtaactggga aagttagaaa
    17281 acctaaagat aacgaaaaat attatggctt attacaagtt gactttgtca atgaccataa
    17341 cgcagaagaa gtgaagaaac gtccgcattt ccaagctttg acaccacttt atccagatga
    17401 gcgtattaaa ttagagacag aaacacaaaa ttattcaacg cgcatcatgg atttagtaac
    17461 accgattggt ttaggtcaac gtggtttaat agtggcgcca cctaaagcag gtaaaacatc
    17521 gttattaaaa gaaatagcga atgcaatcag tacgaacaaa ccagatgcaa agctatttat
    17581 tttgttagtt ggcgagcgtc ctgaagaggt aacagattta gaacgctcag tagaagctgc
    17641 tgaagtcgtt cattcaacgt ttgacgaacc accagaacac catgttaaag tagctgaatt
    17701 attacttgaa cgtgcaaagc gtttagtaga aattggggaa gatgtcatta ttttaatgga
    17761 ttctataacg agattagcac gcgcttataa cttagttatt ccaccaagtg gtcgtacatt
    17821 atcaggtggt ttagatcctg catctttaca caaaccaaaa gcattcttcg gtgcagcgag
    17881 aaatattgaa gcgggtggaa gtttaacaat acttgcaact gcattagttg atacgggttc
    17941 acgtatggac gatatgattt acgaagaatt taaaggaaca ggtaacatgg agttacattt
    18001 agatcgtaaa ttgtctgaac gtcgtatctt ccctgcaatt gatattggca gaagttcaac
    18061 gcgtaaagaa gaattgttga taagtaaatc tgaattagac acattatggc aattaagaaa
    18121 tctattcact gactcaactg actttactga aagatttatt cgcaaactta aaaggtctaa
    18181 gaataatgaa gatttcttca agcagctaca aaagtctgca gaagaaagta ctaaaacggg
    18241 tcgacctata atttaataaa cattatatag gggcttgcgt tttgaattaa ttacctttat
    18301 aattacacag tattgggtaa aaactcacaa ataactctgt tccagatggt tcagggcaaa
    18361 ggagctgaaa ataatgaaac aaggaattca tcctgagtat caccaagtaa tctttttaga
    18421 tactactaca aacttcaaat tcttaagcgg ttctacaaaa acatcttcag aaatgatgga
    18481 gtgggaagat ggaaaagaat acccagttat tcgtttagat atttcatctg attcacatcc
    18541 attctacact ggacgtcaaa aattcgctgc tgcggatgga cgtgtggaac gtttcaacaa
    18601 aaagtttggt cttaaatcaa acaactaata tagtaatcgt taagctgtct gcatcctata
    18661 atggaattgt agacagcttt tttatttata agcgtagtaa gggtatatac ttacgactgt
    18721 gttcaaagaa gtgactcaat accatgttgt taatacataa tgataaattt ccccctttat
    18781 taaagtttgt attgagtaga aacatcgcac gaataagaag caacaaatta gaatagctgt
    18841 atataaatat gcaagacaaa tttataaaat aattgttcat gtacaatatc gatataatta
    18901 tgaaaaagac atttatgaat atttgaaata tgatataata aagcgattat gttttgaaaa
    18961 aggtggaact ctcaatgtat gaaacttacc attccggttg gatagaatgt attacaggta
    19021 gtatgtttag cggtaaatcc gaagaactaa taagacgtct aagaaggggt atatacgcta
    19081 aacaaaaagt cgttgtattt aaaccggcga ttgatgatcg atatcataaa gaaaaagtgg
    19141 tctctcataa tggtaatgcc attgaagcga ttaatatttc taaagcaagt gaaattatga
    19201 cgcacgactt aactaatgtt gatgttatag gaatagacga agtgcaattt tttgacgatg
    19261 aaattgtaag tatagtagaa aaactatcag cagatggaca ccgtgttatt gttgctggat
    19321 tagatatgga ttttaggggc gaaccgttcg aaccaatgcc taaattgatg gctgtcagtg
    19381 aacaagtaac gaaacttcaa gcagtttgtg ctgtttgtgg ttcttcatct agtagaacgc
    19441 aacgattaat aaatggtaaa ccagctaaaa tcgatgatcc gattatctta gtaggtgcca
    19501 atgaaagtta tgagccacgt tgtcgcgcgc accatatcgt agcacctagt gataataata
    19561 aggaggaatt ataagtgttt gatcaattag atattgtaga agaaagatac gaacagttaa
    19621 atgaactgtt aagtgaccca gatgttgtaa atgattcaga taaattacgt aaatattcta
    19681 aagagcaagc tgatttacaa aaaactgtag atgtttatcg taactataaa gctaaaaaag
    19741 aagaattagc tgatattgaa gaaatgttaa gtgagactga tgataaagaa gaagtagaaa
    19801 tgttaaaaga ggagagtaat ggtattaaag ctgaacttcc aaatcttgaa gaagagctta
    19861 aaatattatt gattcctaaa gatcctaatg atgacaaaga cgttattgta gaaataagag
    19921 cagcagcagg tggtgatgag gctgcgattt ttgctggtga tttaatgcgt atgtattcaa
    19981 agtatgctga atcacaagga ttcaaaactg aaatagtaga agcgtctgaa agtgaccatg
    20041 gtggttacaa agaaattagt ttctcagttt ctggtaatgg cgcgtatagt aaattgaaat
    20101 ttgaaaatgg tgcgcaccgc gttcaacgtg tgcctgaaac agaatcaggt ggacgtattc
    20161 atacttcaac agctacagtg gcagttttac cagaagttga agatgtagaa attgaaatta
    20221 gaaatgaaga tttaaaaatc gacacgtatc gttcaagtgg tgcaggtggt cagcacgtaa
    20281 acacaactga ctctgcagta cgtattaccc atttaccaac tggtgtcatt gcaacatctt
    20341 ctgagaagtc tcaaattcaa aaccgtgaaa aagcaatgaa agtgttaaaa gcacgtttat
    20401 acgatatgaa agttcaagaa gaacaacaaa agtatgcgtc acaacgtaaa tcagcagtcg
    20461 gtactggtga tcgttcagaa cgtattcgaa cttataatta tccacaaagc cgtgtaacag
    20521 accatcgtat aggtctaacg cttcaaaaat tagggcaaat tatggaaggc catttagaag
    20581 aaattataga tgcactgact ttatcagagc agacagataa attgaaagaa cttaataatg
    20641 gtgaattata aagaaaagtt agatgaagca attcatttaa cacaacaaaa agggtttgaa
    20701 caaacacgag ctgaatggtt aatgttagat gtatttcaat ggacgcgtac ggactttgta
    20761 gtccacatgc atgatgatat gccgaaagcg atgattatga agttcgactt agcattacaa
    20821 cgtatgttat taggagagcc tatacagtat atagttggct ttgcctcatt ttatggtaga
    20881 acgtttgatg taaactcaaa ttgtttgata ccaagacctg aaactgaaga agtaatgttg
    20941 catttcttac aacagttaga agatgatgca acaatcgtag atatcggaac gggtagtggt
    21001 gtacttgcaa ttactttgaa atgtgaaaag ccggatttaa atgttattgc tactgatatt
    21061 tcacttgaag caatgaatat ggctcgtaat aatgctgaga agcatcaatc acaaatacaa
    21121 tttttaacag gggatgcatt aaagccctta attaatgaag gtatcaagtt gaacggcttg
    21181 atatctaatc caccatatat agatgaaaaa gatatggtta cgatgtctcc aacggttacg
    21241 agattcgaac cacatcaggc attgtttgca gataaccatg gatatgctat ttatgaatca
    21301 atcattgaag atttacctca cgttatggaa aaaggcagcc cagttgtttt tgaaattggt
    21361 tacaatcaag gtgaggcact taaatcaata attttaaata aatttcctga caaaaaaatc
    21421 gacattatta aagatataaa tggccacgat cgaatcgtct catttaaatg gtaattagaa
    21481 gttatgcctt tgctatgatt agttaagtgc atagcttttt gctttatatt atgataaata
    21541 agaaaggcgt gattaagttg gatactaaaa tttgggatgt tagagaatat aatgaagatt
    21601 tacagcaata tcctaaaatt aatgaaataa aagacattgt tttaaacggt ggtttaatag
    21661 gtttaccaac tgaaacagtt tatggacttg cagcaaatgc gacagatgaa gaagctgtag
    21721 ctaaaatata tgaagctaaa ggccgtccat ctgacaatcc gcttattgtt catatacaca
    21781 gtaaaggtca attaaaagat tttacatata ctttggatcc acgcgtagaa aagttaatgc
    21841 aggcattctg gccgggccct atttcgttta tattgccgtt aaagctaggc tatctatgtc
    21901 gaaaagtttc tggaggttta tcatcagttg ctgttagaat gccaagccat tctgtaggta
    21961 gacaattatt acaaatcata aatgaacctc tagctgctcc aagtgctaat ttaagtggta
    22021 gaccttcacc aacaactttc aatcatgtat atcaagattt gaatggccgt atcgatggta
    22081 ttgttcaagc tgaacaaagt gaagaaggat tagaaagtac ggttttagat tgcacatctt
    22141 ttccttataa aattgcaaga cctggttcta taacagcagc aatgattaca gaaatacttc
    22201 cgaatagtat cgcccatgct gattataatg atactgaaca gccaattgca ccaggtatga
    22261 agtataagca ttactcaccc aatacaccac ttacaattat tacagatatt gagagcaaaa
    22321 ttggaaatga cggtaaagat tggtcttcta tagcttttat tgtgccgagt aataaggtgg
    22381 cgtttatacc aagtgaagcg caattcattc aattatgtca ggatgacaat gatgttaaac
    22441 aagcaagtca taatctttat gatgtgttac attcacttga tgaaaatgaa aatatttcag
    22501 cggcgtatat atacggcttt gagctgaatg ataatacaga agcaattatg aatcgcatgt
    22561 taaaagctgc aggtaatcac attattaaag gatgtgaact atgaagattt tattcgtttg
    22621 tacaggtaac acatgtcgta gcccattagc ggaaagtatt gcaaaagagg ttatgccaaa
    22681 tcatcaattt gaatcaagag gtatattcgc tgtgaacaat caaggtgttt cgaattatgt
    22741 tgaagactta gttgaagaac atcatttagc tgaaacgacc ttatcgcaac aatttactga
    22801 agcagatttg aaagcagata ttattttgac gatgtcgtat tcgcacaaag aattaataga
    22861 ggcacacttt ggtttgcaaa atcatgtttt cacattgcat gaatatgtaa aagaagcagg
    22921 agaagttata gatccatacg gtggaacaaa agaaatgtat gtacatacct atgaagaact
    22981 tgtaagttta attttaaaat taaaagatat tatttgctag taggaattct gtataattag
    23041 tatatgttaa cagttaaata agttataata gaggagtgta gcactttgcc atattccttt
    23101 tttattttta ggaagggtgg gcttttatga aagatttgac aatgttatta gacgaattaa
    23161 aagatatgtc cttttttaat aaaggagata tatgtttaat tggatgttca acatcggaag
    23221 tcattggcga aaaaataggg actgtcggtt cgatggaagt tgcagaaact atttttaatg
    23281 cattagatgt tgtgagtaaa gagacaggcg ttacttttgc ttttcaagga tgtgaacata
    23341 tcaacagagc gataacaata gaaaaatctc agtataatcc attaacaatg gaagaagttt
    23401 ccgtcgttcc agatgtacat gctggaggga gtttggcaac atacgctttt cagcatatga
    23461 aggatccgat cgtggtcgaa catattactg ttccttgtgg aattgatatt ggtcaaacgt
    23521 taataggcat gcatattaaa catgtatgtg tacctgtaag gacctcagta aagcaagtag
    23581 gacaggcaat tgtaactatt gccacatcta ggccgaaaaa gattggtggc gaacgtgcta
    23641 aataccaata agtttttttg aaagaagggg agattttatg tcttatatca ccaagcaaga
    23701 taaagttatc gcagaagcaa tcgagagaga atttcagaga caaaatagca acatagagtt
    23761 aatcgcatcg gaaaattttg tatcggaagc ggttatggaa gcacaaggtt cagtgttgac
    23821 taataagtat gctgaaggct atccaggacg ccgatattat ggtggctgtg agtttgtaga
    23881 tgttactgaa agcatcgcaa ttgatcgtgc taaagcattg tttggagctg aacatgtcaa
    23941 tgttcaacca cattcaggtt cacaagcgaa catggctgtt tacttagttg cattaggaat
    24001 gggcgacaca gttttaggta tgaatttgag tcatggtggt cacttgacac atggagcgcc
    24061 tgttaatttt agtggtaaat tctacaattt cgttgaatat ggagtagata aagacacaga
    24121 acgaatcaat tatgatgaag ttcgtaaatt agcgttagag cataagccta agcttattgt
    24181 ggcaggagca tcagcatatt caagaacaat tgacttcaaa aagtttaaag aaatcgcaga
    24241 tgaagtaaac gctaagttaa tggtagacat ggcacatatt gcaggattag tagcggcagg
    24301 tttacatcca aatccagtag aatatgctga ttttgtaaca actacaacac acaaaacatt
    24361 acgcggacca cgtggtggta tgattttatg taaggaagaa tataaaaaag acatagataa
    24421 aacaattttc cctggtattc aaggtggacc tcttgagcat gttattgcag caaaagcagt
    24481 tgcttttgga gaagcgttag aaaataattt caaaacgtat caacaacaag tggttaaaaa
    24541 cgcaaaagtt cttgcagaag cattaattaa tgaaggattt agaattgttt ctggcggtac
    24601 agataatcac ttagtagctg ttgatgtaaa agggtctata ggacttactg gtaaagaagc
    24661 tgaagagact ttagattcag ttggtatcac atgtaacaaa aataccattc cgttcgatca
    24721 agaaaaacct tttgtaacga gtggtatacg tttaggtaca cctgctgcaa caacgcgtgg
    24781 atttgatgaa aaagcttttg aggaagttgc aaaaatcatc agtttagcat tgaaaaatag
    24841 taaagatgaa gaaaaattac aacaagctaa agaacgcgtt gcgaaattaa cagctgaata
    24901 tcctctatat caataaacat acacaaagat aggagtaatt tttatgagta aagtacacgt
    24961 tttcgatcat ccattaattc aacacaaact aagctatatt agagatgtaa atacaggtac
    25021 taaagaattt agagaacttg tagacgaagt tggtatgttg atggcatatg aagtaactag
    25081 agacttagag cttcaggatg ttgatattga aacgcctgta actaaaatga cggctaaacg
    25141 attagctggt aaaaaattag ctatcgttcc aattttaaga gctggtttag gtatgactga
    25201 tggtatttta agtttagtac ctgcagccag agttggacat atcggtttat atcgagatcc
    25261 cgaaacatta aaggctgtag agtattttgc taagttacct caagatatta ctgaaagaca
    25321 aatcattgtt gttgatccta tgttagcaac aggcgcatca gcaattgaag ctattacttc
    25381 attgaagaaa cgtggcgcta aaaatattcg cttcatgtgt ttaattgcag caccagaagg
    25441 cgtagaaaag atgcatgaag cacatccaga tgtagatatt tatattgctg cacttgatga
    25501 aaagctaaat gacaaagcat atatcacacc agggttaggc gacgctggag atagattgtt
    25561 tggtactaaa tagaagaatc ggagataagt agtttgatga aaaagattat gaccatattt
    25621 ggaacaagac ccgaagccat aaagatggct cctttagtta aagctttgga acaagaaaaa
    25681 atgctcgagc caattgttgt tgttacagca cagcatagag aaatgcttga ttcagtatta
    25741 agtacctttg aaatcaaacc aaaatacgat ttgaatatta tgaaatcagg acagacactt
    25801 tctgagataa cttctaaatc aatcactcaa ttagaacaag tgattcaatt agaaaaacca
    25861 gacatggtac ttgtacatgg tgatacgatg acgacatttg caggtggttt agcggcattt
    25921 tataaccaag tgccgattgg acacgttgaa gcagggctta gaagttatga taaatattca
    25981 ccttttccag aagaggtgaa tagacaatta gttggtgtat tagctgactt acattttgca
    26041 cctactaaaa atgctgcatc gcatttgtta agtgaaggga aatattcaga aagtgttgtt
    26101 gtcactggta atactgcgat tgatgctatg aaatatacag ttgatgacaa ttataaatct
    26161 aacatcatgg ataaatacca tgacaaaaaa ttcattctta tgacagcaca ccgacgagaa
    26221 aatatcggga aacccatgga aaatatattt aaagcggtta gacgcttaat agatgagtat
    26281 actgatttag cactggtata tccaatgcat aaaaacccga aagttcgaga ggtggctcaa
    26341 aagattttag gtagtcatga cagaattgaa ttgattgaac ctttggacgt agtagatttc
    26401 cataattttg ctaaaaaatc ttattttatt ttgacagatt caggtggaat tcaggaagaa
    26461 gcgccatcat ttaataagcc agttttagtt ttgcgaagtg ttacagaacg tcctgaaggt
    26521 gttgaagctg gcacattaaa agtcattgga acaaataagc aaaatgtgta tcaagctgca
    26581 aaagaattaa tcgatgatga gagattatat catcaaatgt ctgaagcatc taatccttac
    26641 ggtgatggat ttgcttcaga aagaatagtt aatcatatta agtattattt gaatttaatc
    26701 actgaaaaac caagcgattt ctgaggaaaa tagacttttt gtctaattgt gtctaaattg
    26761 tgtctaacta tgcttatttt tttacaacaa tttgaataga attgacacca caaatcgcct
    26821 atattatcat ttagtttgta tgagtggaac ggttttcatg attgatatca gtcacacgga
    26881 ggcatataaa atgagtcgtt tctacaacat ttttaaacag ttcattcaat attattttta
    26941 tctaataata atattgggag gattatacct ttatacacac catgcattta tcttaggatt
    27001 aatcattggt gttactggtt ctgcaatcaa cacatgtatt tttgaatgct atttagctaa
    27061 agctaaaaga ccagacacta tgcatatttc aactggaaat atgtggcgat acttagttgc
    27121 aattattgcc tgtatgattt ggtaccttaa taaagcgcat gtaagtatca tcggtataat
    27181 tattggttta atgatttcat atgttgtagt tatcatacgt cctttactaa aggtgagcaa
    27241 ataaattaag aaagaggtga gattatggat cacaaatccc cgctcgtgag ttggaattta
    27301 ttcggttttg atatcgtttt caatttatca agtatattga tgatacttgt tacggcgttt
    27361 cttgtttttc tacttgctat catttgtacg cgtaatttga aaaaaagacc aactggcaaa
    27421 caaaatttcg ttgaatggat ttttgatttc gtgaggggaa tcattgaagg taacatggct
    27481 tggaaaaaag gtggtcaatt ccacttctta gcagtaacgc tgattctgta catttttata
    27541 gctaatatgt taggtcttcc gttttctata gtaacgaaag atcacacatt gtggtggaaa
    27601 tcaccgacag ctgatgcaac agtgacttta acgttgtcta caacgataat actgttaact
    27661 cacttttatg gaattaaaat gcgtggtacg aaacaatatc ttaaaggtta tgtacagccg
    27721 ttttggccat tggcaattat taatgttttt gaagagttca cttcaacatt aacgcttggt
    27781 ctgcgtttgt acggtaacat atttgcaggt gagatactat taacattact tgctggctta
    27841 ttctttaacg aaccagcatg gggttggatt attagtatcc caggattaat tgtttggcaa
    27901 gcattttcaa tatttgtagg aacaatccaa gcatatatct ttattatgct ttcgatggtt
    27961 tatatgtcac ataaagtggc agatgaacac taaaaatttc aataattata tacaatcaca
    28021 ggaggaaatt aaattatgaa tttaatcgca gcagcaatcg caattggttt atcagcatta
    28081 ggagcaggta tcggtaacgg tttaatcgtt tcaagaacag ttgaaggtgt agcacgtcaa
    28141 ccagaagcac gtggtcaatt aatgggtatc atgttcattg gtgtaggttt agttgaggca
    28201 ttacctatca tcggtgtagt aattgcattc atgacatttg ctggataatt aacagataaa
    28261 agaggtcggg acaaagcgca taggacataa ttcatgatgc atatatagta atatctttga
    28321 actttattaa atagttgaga tatgaacgca ccatgcctat cgcataaatt cagtaggtcc
    28381 taacctcgtc gtttttttct atataacact agcgattatt ttaatgaaag gagtgtcatg
    28441 aacccgtgac tgaaacagct aacttattcg ttcttggtgc agctggaggc gttgagtggg
    28501 gtactgtgat tgtacaggtc ctaactttca tcgtgttact tgcgttactt aaaaagttcg
    28561 catggggtcc attgaaagat gtaatggata aacgtgaaag agatattaac agagatatcg
    28621 atgacgcaga acaagctaag ttaaatgcac agaaacttga agaagaaaat aaacaaaaac
    28681 ttaaagaaac acaagaagaa gttcaaaaga ttttagaaga tgctaaggtt caagcacgtc
    28741 aacagcaaga acaaattatt catgaagcaa acgtacgtgc aaacggtatg attgaaacag
    28801 cacaaagtga aatcaatagc caaaaagaac gtgccattgc agatattaat aatcaagtat
    28861 ctgaactatc agtgttaatt gcttctaaag ttcttagaaa agaaatttct gaacaagacc
    28921 aaaaagcatt ggttgacaag tatctaaaag aggcaggcga taaataatgg taaaagtagc
    28981 taacaagtat gctaaagcat tatttgacgt gtcattagat acaaataatt tagagactat
    29041 taatgaagaa ttaacagtta taaatgaagc agtaaaagat aaaattgagc aattgagaat
    29101 ggttgacagt aatccaactc aaacagcaga acaacgtcgt gagttgatta atggtgtatt
    29161 tactgacatt aatccataca ttaaaaatat gatgtacgtg ttagcagata atagacatat
    29221 ctcattaata gctgacgtat tcaaggcgtt ccaaagctta tataacggac actacaatca
    29281 agattttgca acaattgagt caacatatga attgagtcaa gaagagttag ataagattgt
    29341 caaactagta actcaacaaa cgaagttatc taaagttatt gtagatacaa aaattaatcc
    29401 agatttaatt ggtggattta gagttaaagt cggcacaact gtattagatg gtagtgttag
    29461 aaatgatctt gtccaattac aaagaaaatt tagaagagtt aattaattat aaagaggagt
    29521 gacatagatg gccataaaag ctgaagaaat cagtgcatta cttcgctcac aaattgaaaa
    29581 ttatgagtca gaaatgtccg taactgatgt aggtactgta ttacaaattg gtgatggtat
    29641 tgcattaatt cacggattaa atgacgttat ggctggtgag ctagtagaat tccataacgg
    29701 cgtacttggt ttagcccaaa accttgaaga gtcaaacgtg ggtgtggtta ttttaggacc
    29761 atacacaggt attactgaag gtgacgaagt taaacgtact ggtcgtatca tggaagtacc
    29821 agtaggtgaa gaactaatcg gaagagttgt taatccatta ggacaaccta ttgatggaca
    29881 aggaccgatt aacacaacta aaacacgtcc agtagagaaa aaagctactg gtgtaatgga
    29941 tcgtaaatca gtagatgagc cattacaaac aggtatcaaa gcaattgatg ctttagtacc
    30001 aattggtaga ggtcaacgtg agttaatcat cggtgaccgt caaacaggta aaacaacaat
    30061 tgcaattgac acaattttga accaaaaaga tcaaggtacg atttgtatct atgttgctat
    30121 tggtcaaaaa gattcaacag taagagcaaa tgttgaaaag ttaagacaag caggcgcttt
    30181 agactacact attgttgtag cagcatcagc ttctgaacct tctccattat tatatattgc
    30241 accatattca ggtgtaacaa tgggtgaaga attcatgttt aacggtaaac atgttttaat
    30301 cgtttatgat gatttaacta aacaagcagc agcttatcgt gagttatcat tattattacg
    30361 tagacctcca ggtcgtgaag catacccagg tgacgtattc tacttacata gtagattatt
    30421 agaaagagca gcaaaattaa acgatgactt aggtggcggt tcaattactg cattaccaat
    30481 tatcgaaaca caagctggtg atatttcagc ttatgtacca acaaacgtta tttcaattac
    30541 agatggacaa atcttcttac aatctgattt attcttctca ggtgtaagac cagcgattaa
    30601 tgccggacaa tctgtatctc gtgttggtgg atctgcacaa attaaagcaa tgaagaaagt
    30661 tgctggtacg ttacgtcttg acttagcgtc atacagagaa cttgaatcat ttgcacaatt
    30721 cggttcagac cttgatgaat ttactgcaag taaattagaa cgtggtaaac gtactgttga
    30781 agtcttaaaa caagatcaaa acaaaccatt accagtcgaa caccaagtgt tgattattta
    30841 tgcattaaca aaaggatatt tagatgatat tccagttgta gatatcacac gttttgaaga
    30901 cgagttaaac cactgggcag aatcaaatgc tactgaactg ttaaatgaaa tcagagaaac
    30961 tggtggctta ccagatgctg agaagtttga cacagcaatt aacgaattca aaaaaagctt
    31021 tagcaaatct gaataataaa caagtttagt ataaggtggt gagatagtgg cttctcttaa
    31081 agaaatagat actcgaataa aatcaaccaa aaaaatgaag cagattacga aagcgatgaa
    31141 catggtatca agttcaaaac ttcgtagagc tgaaaaaaat acaaaacaat tcacaccata
    31201 tatggataaa atgcaagatg caattactgc agttgcaggg gcaagtagca atacaaacca
    31261 tccaatgcta agacctcgta aaattactag aagtggatat ttagttatca cgagtgataa
    31321 aggtttagca ggtgcatata gtgcaaacgt gcttaaaaaa ttgattactg atattgaagc
    31381 gaaacatcaa gatagtagcg aatacagtat tgtagtttta gggcaacaag gtgttgattt
    31441 cctaaaaaat agaggttatg acattgagta ttctcaagta gacgtacctg atcaaccttc
    31501 tttcaaatct gttcaagcac tagctaacca tgctatagac ttatacagtg aagaagaaat
    31561 tgatgaatta aatatatact atagtcatta tgtcagcgtt cttgaaaaca agcctacatc
    31621 tagacaagta ttaccattat ctcaagagga ttctagtaag gggcatggtc atttgtcttc
    31681 ttatgaattt gagccagata aagaatctat cttaagtgta atcttgcctc aatatgttga
    31741 gagtttgatt tacggaacaa tattagacgc aaaagcaagt gagcatgcaa cacgtatgac
    31801 tgcgatgaaa aatgccactg ataatgcaac tgaacttatt gatgacttat cattagaata
    31861 taacagagcg agacaagcag aaattacgca acaaattact gaaattgttg gtggttccgc
    31921 agcgcttgaa taatatttaa aggaggaaaa tagcatggga attggccgtg taactcaagt
    31981 tatgggtcct gtaattgatg ttcgatttga acataacgaa gttcctaaaa ttaataacgc
    32041 cttggttatt gatgtgccta aagaagaagg tacaatacaa ctaacattag aagttgcgct
    32101 gcaattaggt gacgacgttg ttcgtacaat tgcgatggat tcaactgatg gtgtccaaag
    32161 aggcatggat gtaaaagata caggcaaaga aattagtgta cctgttggtg acgaaacatt
    32221 aggtcgtgta tttaatgtac taggtgaaac aattgacctt aaagaagaaa ttagtgattc
    32281 tgttcgccgc gatcctatcc atcgtcaagc accagcattc gatgaacttt caacagaagt
    32341 tcaaatttta gaaacaggta ttaaagtagt agatttacta gcaccttata ttaaaggtgg
    32401 taaaatcgga ttgttcggtg gtgccggtgt aggtaaaaca gtattaatcc aagaattaat
    32461 taacaacatc gctcaagagc acggtggtat ttctgtattc gccggtgtag gtgaacgtac
    32521 tcgtgaaggt aacgatttat acttcgaaat gagtgacagt ggtgtaatta agaaaacagc
    32581 catggtattc gggcaaatga atgagccacc tggtgcacgt atgcgtgttg cattatctgg
    32641 tttaacaatg gctgaatatt tccgtgacga acaaggtcaa gacgtattat tattcatcga
    32701 taacattttc agatttacac aagctggttc tgaggtatct gcattattag gtcgtatgcc
    32761 ttctgcagta ggttaccaac caacacttgc tactgaaatg ggacaattac aagaacgtat
    32821 tacgtctaca acaaaaggat cagttacttc tattcaagcg gtattcgtac ctgccgatga
    32881 ctatactgac ccagcgcctg cgacagcgtt tgcccattta gatgcaacta caaacttaga
    32941 acgtaaatta actgaaatgg gtatttatcc agccgtggat ccattagcgt ctacatcaag
    33001 agcattggaa ccatcaattg taggtcaaga acattatgaa gtagctcgtg atgtacaatc
    33061 aacacttcaa aaataccgtg aattacaaga tatcattgct atcttaggta tggacgaatt
    33121 atctgatgaa gataaacaaa cagttgaacg cgcacgtaga attcaattct tcttatctca
    33181 aaacttccac gtagcggaac aatttactgg tcaaaaaggt tcttatgtac ctgttaagac
    33241 aacagttgca aactttaaag atatcttaga tggtaaatat gaccatattc cagaagatgc
    33301 attccgttta gttggtagca tggatgatgt tattgcaaaa gctaaagata tgggtgttga
    33361 agtataacaa ttaggaggaa tggataatga atacattaaa cctagatatt gtcactccta
    33421 atggttctgt ttacaatcgt gataatgttg aactcgttgt tatgcaaaca acagctggtg
    33481 agataggtgt catgagtgga catattccaa ctgtagctgc tttaaaaaca ggctttgtaa
    33541 aagtgaaatt tcacgatgga actgaatata ttgctgtaag cgatggcttt gttgaagtta
    33601 gaaaagataa agtttcaatc attgttcaga ctgcagaaac tgcaagagaa attgatgttg
    33661 aaagagctaa attagccaaa gcaagagcag agtctcactt ggaaaatgat gacgacaata
    33721 ctgatattca tagagccgaa agagctttag agagagcaaa taaccgtttg cgtgtggctg
    33781 aattaaaata gtaaataaag ggtcgaagat gtgatttcat atcttcgacc cttttttgaa
    33841 ttatattgat ttaaagatac aaaacatgag aggggggaag gaattgataa agaaccatta
    33901 aagatttatg atgtagtggt tctttatcat taaacacagc taatgtgtat ttaaaaatag
    33961 gaatacatga gtaaaactca tgtataagaa atactaattt ctaaagaaaa agtatttctt
    34021 tatgttgggg ccccgtcaac tactgccaaa tacaacacta tagagtctag acattgattt
    34081 atgtccgact cccaagaata gttttacttt tttacaatca ctaatagatt gctaaaatca
    34141 aaatttcctt caccactatc tacagtcgac atttcatttt ttgaaattat ctacattttt
    34201 tcataccaag atattttata gttatgatat ttatgtaaaa agaattatat agtaagttag
    34261 cttaaacttt actaaaaacg ggtattaaac tttgtatcat tatttaaatt tttcatgtac
    34321 aatgtaatac agtaatctta tgaggtgata aaatggatta tatcggacaa tatgcagtta
    34381 tccatttagt gttacatgtt gtatgtattt gtattgccta ttgggcttta caatcaatta
    34441 gattagatca attttttaaa aaaggatacg ccactcaatt acaagtgtgt atgatatttg
    34501 ttgctatttt attaggcact gcagtaagca attttattgt agatttgtta caatactcga
    34561 cgcaggtaaa atatttaata aaataagtct aactctatga tttgtaatca aaactagata
    34621 taattaaata atgacttaaa ataattttaa aatagggaaa tgtaaagtaa taggagttct
    34681 aagtggagga tttacgatgg ataaaatagt aatcaaaggt ggaaataaat taacgggtga
    34741 agttaaagta gaaggtgcta aaaatgcagt attaccaata ttgacagcat ctttattagc
    34801 ttctgataaa ccgagcaaat tagttaatgt tccagcttta agtgatgtag aaacaataaa
    34861 taatgtatta acaactttaa atgctgacgt tacatacaaa aaggacgaaa atgctgttgt
    34921 cgttgatgca acaaagactc taaatgaaga ggcaccatat gaatatgtta gtaaaatgcg
    34981 tgcaagtatt ttagttatgg gacctctttt agcaagacta ggacatgcta ttgttgcatt
    35041 gcctggtggt tgtgcaattg gaagtagacc gattgagcaa cacattaaag gttttgaagc
    35101 tttaggcgca gaaattcatc ttgaaaatgg taatatttat gctaatgcta aagatggatt
    35161 aaaaggtaca tcaattcatt tagattttcc aagtgtagga gcaacacaaa atattattat
    35221 ggcagcatca ttagctaagg gtaagacttt aattgaaaat gcagctaaag aacctgaaat
    35281 tgtcgattta gcaaactaca ttaatgaaat gggtggtaga attactggtg ctggtacaga
    35341 cacaattaca atcaatggtg tagaatcatt acatggtgta gaacatgcta tcattccaga
    35401 tagaattgaa gcaggcacat tactaatcgc tggtgctata acgcgtggtg atatttttgt
    35461 acgtgatgca atcaaagaac atatggcgag tttagtctat aaactagaag aaatgggcgt
    35521 tgaattggac tatcaagaag atggtattcg tgtacgtgct gaaggggaat tacaacctgt
    35581 agacatcaaa actctaccac atcctggatt cccgactgat atgcaatcac aaatgatggc
    35641 attgttatta acggcaaatg gtcataaagt cgtaaccgaa actgtttttg aaaaccgttt
    35701 tatgcatgtt gcagagttca aacgtatgaa tgctaatatc aatgtagaag gtcgtagtgc
    35761 taaacttgaa ggtaaaagtc aattgcaagg tgcacaagtt aaagcgactg atttaagagc
    35821 agcagccgcc ttaattttag ctggattagt tgctgatggt aaaacaagcg ttactgaatt
    35881 aacgcaccta gatagaggct atgttgactt acacggtaaa ttgaagcaat taggtgcaga
    35941 cattgaacgt attaacgatt aattcagtaa attaatataa tggaggattt caaccatgga
    36001 aacaattttt gattataacc aaattaaaca aattatacct cacagacagc catttttatt
    36061 aattgataaa gtagttgaat atgaagaagg tcaacgttgt gtggctatta aacaagtatc
    36121 aggaaacgaa ccattctttc aagggcattt tcctgagtat gcggtaatgc caggcgtatt
    36181 aattactgaa gcgttagctc aaacaggtgc ggtagctatt ttaaatagtg aagaaaataa
    36241 aggtaaaatc gctttatttg ctggtattga taaatgtcgt tttaaacgtc aagtagtacc
    36301 tggtgatact ttaacgttgg aagtagaaat cactaaaatt aaaggaccaa taggtaaagg
    36361 taatgctaaa gctactgtcg atggtcaact tgcttgtagt tgtgaactta catttgcaat
    36421 tcaagatgta aaataaaaca aaaaaaacat tcaaagattt aatgtgttgg cataatcttt
    36481 gaatgttttt tattttactc ttctaatttt tcatccttta actttggttt agactgcatc
    36541 attcgattaa atgatttttt taattcttca ccagataatc catcatcaat aagttgttct
    36601 aataaacttt cagcatactg ttgacgtaaa gtgtaaatat gacattcaaa tacgatagac
    36661 attgttgttt taaaattttt aatacttttc acagtagatt caaataaagc tggatcattg
    36721 gctggacgcg tgatgacgac acgaatatcc aataactgtt cattgtcttg atattcctta
    36781 aatgtttcat aatgctcatt agaaatgaca atttcaagca accaacccgt gccactttcc
    36841 tctttattaa ttattacgcc atcttctaat tcatattcta taattctgcc gtgttcattc
    36901 acaatttgaa accttactgc tttaaaagtt ttcatattcc cacatcccat tagtttaata
    36961 tttatgattt ttgaattatt tagaagattt aaggtcattt catgactaat ttctttaaat
    37021 ataacaaata acgagatgct atagctatta attatataaa atttgccatt ttgaccattt
    37081 ttaagctagt aattaagata taggcatagg aggtgaatca cacactacat gctaaataaa
    37141 atcgtaattg tcgggagact gacgaaagac gcacaaatat ttgaaaagga ggatagaaaa
    37201 attgcaacgt tttgtgttgc aacgcaccga aattataaag atgaaaatgg agaaatcgtc
    37261 tgtgattact tattctgtaa agcatttggc aagttagctt ctaatataga aaaatatact
    37321 aatcaaggta cattggttgg tataactggt caaatgagat caagaaagta tgataaagac
    37381 ggacaaacac actttgtcac tgaattatat gttgaaacaa taaaatttat gtcccctaaa
    37441 tcccaaaata atgaaattct ctcagatagt atttagatat tgactctcaa aatatagata
    37501 atcat
//
LOCUS       DAMQYT010000009        37459 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMQYT010000009 DAMQYT010000000
VERSION     DAMQYT010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 37459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37459
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.009"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..742
                     /locus_tag="PGA33_000352"
     CDS             <1..742
                     /locus_tag="PGA33_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDG6472191.1"
                     /translation="IHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGYINENYIEL
                     LDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKNKLQSEQVE
                     VVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKKLQKYFQKQ
                     FSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIEDGKVSKYH
                     SVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(734..985)
                     /locus_tag="PGA33_000353"
     CDS             complement(734..985)
                     /locus_tag="PGA33_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472192.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLVIIHMGILAHSTYVYLY"
     gene            1374..2639
                     /gene="femX"
                     /locus_tag="PGA33_000354"
                     /gene_synonym="fmhB"
     CDS             1374..2639
                     /gene="femX"
                     /locus_tag="PGA33_000354"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HDG6472193.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            2756..5923
                     /locus_tag="PGA33_000355"
     CDS             2756..5923
                     /locus_tag="PGA33_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDG6472194.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVATIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(6110..6427)
                     /gene="mspA"
                     /locus_tag="PGA33_000356"
     CDS             complement(6110..6427)
                     /gene="mspA"
                     /locus_tag="PGA33_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HDG6472195.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(6672..6836)
                     /locus_tag="PGA33_000357"
     CDS             complement(6672..6836)
                     /locus_tag="PGA33_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472196.1"
                     /translation="MSLENQLAELKYDYVRLQGDIEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            6969..7877
                     /locus_tag="PGA33_000358"
     CDS             6969..7877
                     /locus_tag="PGA33_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDG6472197.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFVMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVYVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     TFIGMTTNLTIIISIIILYVYQAVFV"
     gene            8048..8179
                     /locus_tag="PGA33_000359"
     CDS             8048..8179
                     /locus_tag="PGA33_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472198.1"
                     /translation="MYGAYLIYEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(8296..9159)
                     /locus_tag="PGA33_000360"
     CDS             complement(8296..9159)
                     /locus_tag="PGA33_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HDG6472199.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(9340..10227)
                     /locus_tag="PGA33_000361"
     CDS             complement(9340..10227)
                     /locus_tag="PGA33_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6472200.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            10293..10388
                     /locus_tag="PGA33_000362"
     CDS             10293..10388
                     /locus_tag="PGA33_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472201.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            10414..12549
                     /locus_tag="PGA33_000363"
     CDS             10414..12549
                     /locus_tag="PGA33_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDG6472202.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            12662..13996
                     /locus_tag="PGA33_000364"
     CDS             12662..13996
                     /locus_tag="PGA33_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDG6472203.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(14188..14577)
                     /locus_tag="PGA33_000365"
     CDS             complement(14188..14577)
                     /locus_tag="PGA33_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472204.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            14934..15242
                     /gene="rpsJ"
                     /locus_tag="PGA33_000366"
     CDS             14934..15242
                     /gene="rpsJ"
                     /locus_tag="PGA33_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDG6472205.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            15270..15932
                     /gene="rplC"
                     /locus_tag="PGA33_000367"
     CDS             15270..15932
                     /gene="rplC"
                     /locus_tag="PGA33_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDG6472206.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            15959..16582
                     /gene="rplD"
                     /locus_tag="PGA33_000368"
     CDS             15959..16582
                     /gene="rplD"
                     /locus_tag="PGA33_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDG6472207.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            16582..16857
                     /gene="rplW"
                     /locus_tag="PGA33_000369"
     CDS             16582..16857
                     /gene="rplW"
                     /locus_tag="PGA33_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDG6472208.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            16890..17723
                     /gene="rplB"
                     /locus_tag="PGA33_000370"
     CDS             16890..17723
                     /gene="rplB"
                     /locus_tag="PGA33_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDG6472209.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            17790..18068
                     /gene="rpsS"
                     /locus_tag="PGA33_000371"
     CDS             17790..18068
                     /gene="rpsS"
                     /locus_tag="PGA33_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDG6472210.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            18097..18450
                     /gene="rplV"
                     /locus_tag="PGA33_000372"
     CDS             18097..18450
                     /gene="rplV"
                     /locus_tag="PGA33_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDG6472211.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            18474..19127
                     /gene="rpsC"
                     /locus_tag="PGA33_000373"
     CDS             18474..19127
                     /gene="rpsC"
                     /locus_tag="PGA33_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDG6472212.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            19130..19564
                     /gene="rplP"
                     /locus_tag="PGA33_000374"
     CDS             19130..19564
                     /gene="rplP"
                     /locus_tag="PGA33_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDG6472213.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            19554..19763
                     /gene="rpmC"
                     /locus_tag="PGA33_000375"
     CDS             19554..19763
                     /gene="rpmC"
                     /locus_tag="PGA33_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDG6472214.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            19787..20050
                     /gene="rpsQ"
                     /locus_tag="PGA33_000376"
     CDS             19787..20050
                     /gene="rpsQ"
                     /locus_tag="PGA33_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDG6472215.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            20082..20450
                     /gene="rplN"
                     /locus_tag="PGA33_000377"
     CDS             20082..20450
                     /gene="rplN"
                     /locus_tag="PGA33_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDG6472216.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            20486..20803
                     /gene="rplX"
                     /locus_tag="PGA33_000378"
     CDS             20486..20803
                     /gene="rplX"
                     /locus_tag="PGA33_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDG6472217.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            20830..21369
                     /gene="rplE"
                     /locus_tag="PGA33_000379"
     CDS             20830..21369
                     /gene="rplE"
                     /locus_tag="PGA33_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDG6472218.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            21392..21577
                     /locus_tag="PGA33_000380"
     CDS             21392..21577
                     /locus_tag="PGA33_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDG6472219.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            21609..22007
                     /gene="rpsH"
                     /locus_tag="PGA33_000381"
     CDS             21609..22007
                     /gene="rpsH"
                     /locus_tag="PGA33_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDG6472220.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            22032..22568
                     /gene="rplF"
                     /locus_tag="PGA33_000382"
     CDS             22032..22568
                     /gene="rplF"
                     /locus_tag="PGA33_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDG6472221.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            22599..22958
                     /gene="rplR"
                     /locus_tag="PGA33_000383"
     CDS             22599..22958
                     /gene="rplR"
                     /locus_tag="PGA33_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDG6472222.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            22979..23479
                     /gene="rpsE"
                     /locus_tag="PGA33_000384"
     CDS             22979..23479
                     /gene="rpsE"
                     /locus_tag="PGA33_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDG6472223.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            23496..23675
                     /gene="rpmD"
                     /locus_tag="PGA33_000385"
     CDS             23496..23675
                     /gene="rpmD"
                     /locus_tag="PGA33_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDG6472224.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            23692..24132
                     /gene="rplO"
                     /locus_tag="PGA33_000386"
     CDS             23692..24132
                     /gene="rplO"
                     /locus_tag="PGA33_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDG6472225.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            24132..25424
                     /gene="secY"
                     /locus_tag="PGA33_000387"
     CDS             24132..25424
                     /gene="secY"
                     /locus_tag="PGA33_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDG6472226.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            25441..26088
                     /locus_tag="PGA33_000388"
     CDS             25441..26088
                     /locus_tag="PGA33_000388"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDG6472227.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            26281..26499
                     /gene="infA"
                     /locus_tag="PGA33_000389"
     CDS             26281..26499
                     /gene="infA"
                     /locus_tag="PGA33_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDG6472228.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            26531..26644
                     /gene="rpmJ"
                     /locus_tag="PGA33_000390"
     CDS             26531..26644
                     /gene="rpmJ"
                     /locus_tag="PGA33_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDG6472229.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            26667..27032
                     /gene="rpsM"
                     /locus_tag="PGA33_000391"
     CDS             26667..27032
                     /gene="rpsM"
                     /locus_tag="PGA33_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDG6472230.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            27056..27445
                     /gene="rpsK"
                     /locus_tag="PGA33_000392"
     CDS             27056..27445
                     /gene="rpsK"
                     /locus_tag="PGA33_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDG6472231.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            27520..28464
                     /locus_tag="PGA33_000393"
     CDS             27520..28464
                     /locus_tag="PGA33_000393"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDG6472232.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            28481..28849
                     /gene="rplQ"
                     /locus_tag="PGA33_000394"
     CDS             28481..28849
                     /gene="rplQ"
                     /locus_tag="PGA33_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDG6472233.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            29387..30196
                     /locus_tag="PGA33_000395"
     CDS             29387..30196
                     /locus_tag="PGA33_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDG6472234.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPYDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDNAEELTRIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            30193..31053
                     /locus_tag="PGA33_000396"
     CDS             30193..31053
                     /locus_tag="PGA33_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDG6472235.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKKKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            31043..31849
                     /locus_tag="PGA33_000397"
     CDS             31043..31849
                     /locus_tag="PGA33_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDG6472236.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFIMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTISLTMIIPIAIILFVLKYSGV"
     gene            31854..32657
                     /gene="truA"
                     /locus_tag="PGA33_000398"
     CDS             31854..32657
                     /gene="truA"
                     /locus_tag="PGA33_000398"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDG6472237.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLGKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            32897..33334
                     /gene="rplM"
                     /locus_tag="PGA33_000399"
     CDS             32897..33334
                     /gene="rplM"
                     /locus_tag="PGA33_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDG6472238.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            33348..33746
                     /gene="rpsI"
                     /locus_tag="PGA33_000400"
     CDS             33348..33746
                     /gene="rpsI"
                     /locus_tag="PGA33_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDG6472239.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            34291..35388
                     /locus_tag="PGA33_000401"
     CDS             34291..35388
                     /locus_tag="PGA33_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDG6472240.1"
                     /translation="MRGDQRTQGEKSRKEGGKIFGSGSRAPIAISILVKDGSYNHDIY
                     YNDIGEYLTREQKLDTLMKHQSIVNLKSLNVLPDKNNDWINQRDINYENYLPMYDSKD
                     IENSIYLDQFNGVNSARDNWVTNFSNEKALVNAKLLVDNYNSEIDRLIDILDSRERIN
                     LVNKDETFISWTRGLTQKFSKGKNISINPERIVKFMHRPFTKKWIVYDKNIMEMPSRY
                     YNIMENTGQVIYIQGQGMNKEFSAMITDILPNFQFIGNGKGFATYKGKDSLRLVDNIS
                     NSFKKKINLNSEEIVYYIYAILHHKYYVNKYSSDLSKGFPRIPILKDVYGFVEIGREL
                     VELHLNYEKQLNWDGVEIIYNNMNPNYKVEK"
     gene            35385..35678
                     /locus_tag="PGA33_000402"
     CDS             35385..35678
                     /locus_tag="PGA33_000402"
                     /inference="COORDINATES: protein motif:HMM:NF037202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472241.1"
                     /translation="MKHPKKGVLDTVIYNTDITVTNIPNKVYNYIVNGKPAIEWIIDQ
                     YNVSIDKKSGILDDPNEFSEDPNYILNLLLSVITVSMKTLGLIDKLPDFEVLD"
     gene            35739..36482
                     /locus_tag="PGA33_000403"
     CDS             35739..36482
                     /locus_tag="PGA33_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472242.1"
                     /translation="MDLLQIEKKGNLTKTVQLMHDEKGSASGESKVNMEFGKQSAVKQ
                     IFDSLLGMSASASASAGINGNITGERVAKTILENTLIYDFLDTVEFRKKKTIIDISEG
                     FKLNIQQDTMTYFATITPIASMFEGSKHLDEEELSFDVSKMNEAIRSIKGYFELIGTR
                     GNENRVFRFNIESFKNNYRIQDLKFMNLTLYSVLVGEISKENLNYDKEFDLNQGAKPS
                     GIGKFKGIRSEATKEDKYQVYDVILAGVK"
     gene            36483..>37459
                     /locus_tag="PGA33_000404"
     CDS             36483..>37459
                     /locus_tag="PGA33_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDG6472243.1"
                     /translation="MEIHVYTGPLCGFNEYINSEVKEYHRFMELIREYNIIVRASDTF
                     GTHDVDGIFDTEIENLVIFSNDFASVTTHVITNFINIVTLGRNIKKIYIQNPPKRVFQ
                     SLNSFHDSEIIEINYEYTTLKKDDVINFYKEQMAGSKIIGQTDAKKQMSIDLYKHTSL
                     ENNKPTVMLYYGPSGVGKTELAKEIAKKYKGNITRIQFSMMQNDESFNYIFGDEHAKV
                     SLSKDLVDRETNVVLIDEFDKVLPIYYNVFYQMFDEGIIEDANYKVDVSNCIFILTSN
                     FNSIEEAIVNIGEPVFSRIDSCIKFLYLTQEEKQKVIENKLNEILSSLN"
BASE COUNT        13711 a         5580 c         7071 g        11097 t
ORIGIN      
        1 cattcattat atagatcagt tagatcggtt tagttttcca ggagatgtta taaaattaca
       61 ttcaggtggg tatcatcata aatatggaac attcaataaa ttaggttata tcaatgaaaa
      121 ttatattgag ctactagatg tagaaaataa tgaaaagttg aaaaagatgg caaaaacgat
      181 agaaggcgga gtcgcttttg ctactcaaat tgttcaagag aagtatgagc aaggctttaa
      241 aaatatttgt ttgcgtacaa atgatataga ggcagttaaa aataaactac aaagtgagca
      301 ggttgaagta gtagggccga ttcaaatgga aagagataca cataaagatg gtaaggtaaa
      361 gtggcaattg ctttatataa tgaatcagga tgatgatgaa attaagccac cattttttat
      421 tcaatgggaa gaaagtgatt ccatgcgtac taaaaaattg caaaaatatt ttcaaaaaca
      481 attttcaatt gaaactgtta ttgtgaaaag taaaaaccga tcacaaacag tatcgaattg
      541 gttgaaatgg tttgatatgg acattgtaga agagaatgac cattacacag atttgatttt
      601 aaaaaatgat gatatttatt ttagaattga agatggtaaa gtttcaaaat atcattcggt
      661 tatcataaaa gacgcacaag caacttcacc atattcaatt tttatcagag gtgctattta
      721 tcgctttgaa ccattagtat aaatatacgt aagtgctatg agcgagaatg cccatatgaa
      781 taatgacaag cacaatggaa agaatcgtta atatattatt taatcgtgat gacttaatta
      841 aaatgaaaaa gattgataat ataaatgtga aaaagataag tataacccgt aaactaaagt
      901 aattcacggt gagaggttga ctcaatgtca taatgattgc aacgatgttc ataattataa
      961 atagacttaa aataattgtt ctcatatcaa acacctcatt gttagattat tgacattata
     1021 acaggggtaa ttgtatatga acattaatgt ggttgcttga ggaaaaattt attcattgaa
     1081 gtcaagttgg ttcattttag aaatgaatat cgtgttagat gatgaaagta tattgaagta
     1141 taggtaacta gttgaaaagt attaattgta cgataacatt aaatttaaca cgaaacatag
     1201 atataaaatg attcacaatt aaaatgggta aatttgaact tgctaaacta ttaattggag
     1261 catggacatt tcaaaaataa gagttcaaat cttacacaag ctctgaatcg acactataag
     1321 atacaaactg tataattaaa ggtattgtta aatagaagga gatatcataa atcatggaaa
     1381 agatgcatat cactaatcag gaacatgacg catttgttaa atcccaccca aatggagatt
     1441 tattacaatt aacgaaatgg gcagaaacaa agaaattaac tggatggtac gcgcgaagaa
     1501 tcgctgtagg tcgtgacggt gaagttcagg gtgttgcgca gttacttttt aaaaaagtac
     1561 ctaaattacc ttatacgcta tgttatattt cgcgtggttt tgttgttgat tatagtaata
     1621 aagaagcgtt aaatgcattg ttagacagtg caaaagaaat tgctaaagct gagaaagcgt
     1681 atgcaattaa aatcgatcct gatgttgaag ttgataaagg tacagatgct ttgcaaaatt
     1741 tgaaagcgct tggttttaaa cataaaggat ttaaagaagg tttatcaaaa gactacatcc
     1801 aaccacgtat gactatgatt acaccaattg ataaaaatga tgatgagtta ttaaatagtt
     1861 ttgaacgccg aaatcgttca aaagtgcgct tggctttaaa gcgaggtacg acagtagaac
     1921 gatctgatag agaaggttta aaaacatttg ctgagttaat gaaaatcact ggggaacgcg
     1981 atggcttctt aacgcgtgat attagttact ttgaaaatat ttatgatgcg ttgcatgaag
     2041 atggagatgc tgaactattt ttagtaaagt tggatccaaa agaaaatata gcgaaagtaa
     2101 atcaagaatt gaatgaactt catgccgaaa ttgctaaatg gcagcagaag atgaaaacat
     2161 ctgaaaagca agctaaaaaa gcgcaaaata tgattaatga tgcgcaaaat aaaattgcta
     2221 aaaatgaaga tttaaaacga gacctagaag ctttagaaaa ggaacatcct gaaggtattt
     2281 atctttctgg tgcactatta atgtttgctg gctcaaaatc atattactta tatggtgcgt
     2341 cttctaatga atttagagat tttttaccaa atcatcatat gcagtatacg atgatgaagt
     2401 atgcacgtga acatggtgca acaacttacg atttcggtgg tacagataat gatccagata
     2461 aagactcaga acattatgga ttatgggcat ttaaaaaagt gtggggaaca tacttaagtg
     2521 aaaagattgg tgaatttgat tatgtattga atcagccatt gtaccaatta attgagcaag
     2581 ttaaaccgcg tttaacaaaa gctaaaatta aaatatctcg taaattaaaa cgaaaataga
     2641 ttaacgactg aaatctgaac gctcataaga ctgtcatttg cgttcagatt tttttacaca
     2701 atatagaatg gttgagtaaa atatttttga atatagtgaa agagggggaa gtactgtgat
     2761 aaaaaagcta ttacaatttt ctttagggaa taagtttgct atctttttaa tggttgtttt
     2821 agttgtcttg ggcggtgtat atgcgagtgc taaattgaaa ttagaattac taccaaatgt
     2881 acaaaatcca gttatttcag ttacaacaac aatgccgggt gcaacgccac aaagtaccca
     2941 agatgaaata agtagtaaaa ttgacaatca agtaagatca ttggcatatg tgaaaaatgt
     3001 taaaacgcaa tccatacaaa atgcttcaat tgtaacagtt gaatatgaaa ataatacaga
     3061 tatggataaa gcagaagaac agcttaaaaa agaaatcgat aaaattaaat ttaaagatga
     3121 agttggtcaa ccagaattaa gacgtaattc gatggatgct tttccggttt tagcatattc
     3181 attttcaaat aaagagaatg acttgaaaaa agtaacgaaa gtactgaatg aacaattaat
     3241 accaaaattg caaacggtag atggtgtgca aaatgcgcaa ttaaatgggc agacgaaccg
     3301 tgaaatcacc cttaaattta agcaaaatga acttgaaaaa tatgggttga ctgctgatga
     3361 tgtagaaaac tatctaaaaa cggcaacaag aacaacgcca cttggattgt tccaatttgg
     3421 tgataaagat aaatcaattg ttgttgatgg tcaatatcaa tctgttgatg cttttaaaaa
     3481 cataaatatt ccattaacgt tggcaggagg acaagggcaa tctcaatccc aaagtgacaa
     3541 taaacaaaat tcagccatgt cagacgttaa tcaggcatca ccacagcaaa attcaaaagc
     3601 gtcagcacca aataatataa gtggtatgcc gacagcgaaa ctaggagatt tagctgatat
     3661 tacagttggt gatgtgcgta cttctatttc taaaacgaat ggaaaggatg cggttaatct
     3721 acaaataact aaagctcaag atgccaatac agttcaagta gccaaagatg tacaacgtaa
     3781 aattgataca ttcgttgatg aaaataaaga ttttaatgtc acaaaaacaa tggatactgc
     3841 aaagcctgtt gagaaatcac tttatacgat ggttgaaaaa gcatcattag gtacaatcgt
     3901 ggcaattata gttattttgc tgtttttaag aaacattcgt acgacggcaa tttctattat
     3961 atcgattccg ttatcacttc ttatggcgct tattgctctg aaattgagtg atgtttcatt
     4021 gaatatacta acgttaggtg cattaacagt agcgattgga cgtgtgatag acgattcgat
     4081 tgtagttgtt gaaaatattt atcgacgctt aacagattca gaagaacaac taaaaggtga
     4141 aaatttaatt atcagtgcga caactgaagt atttaaacca ataatgtcat cgacactagt
     4201 tactattatc gtcttcttac cacttgtgtt tgtatcaggt tcagtaggcg aaatgtttag
     4261 accttttgca ttggctattg catttagttt attagcatcg ttattagtgt caattacact
     4321 cgttccagcg ttggcagcta cactatttaa aaaaggcgtt aaacgtcgta ataaacaaca
     4381 tcaagaagga ttaggtgttg ttagtacaac ttataaaaaa gtattacatt ggtcattaaa
     4441 tcataagtgg attgtaatta tattaagtac attaattttg gttgcaacta ttgtatttgg
     4501 aggaccgaga ctaggcacta gctttatttc agcaggtgac gataaatttt tagctattac
     4561 ttatacaccg aagcctggtg aaacggagca agcagtgttg aatcatgcga aagatgttga
     4621 aaaatattta aaacagaaaa agcatgtaaa aacaattcaa tactcagttg gcggtagtag
     4681 tccagtagat ccaacgggta gtacaaatag tatggcaatc atggttgaat atgataatga
     4741 cacgcctaat tttgatgtag aagcggataa ggttattaaa catgcagatg gctttaaaca
     4801 tcctggagag tggaaaaatc aagatttagg aacaggtgca ggtaataaat ctgtagaggt
     4861 tactgtaaaa ggtccatcaa tggatgccat aaaatcaact gtaaaagata ttgaacagaa
     4921 aatgaaacag gttaaaggac tagccaatgt caaatctgat ttatcgcaaa catatgatca
     4981 gtatgaaatt aaagtcgatc aaaataaagc ggcagaaaat ggtatttctg caagtcaact
     5041 tgcaatgcac ttgaatgaaa acttaccaga aaaaacagtt acgactgtta aagaaaatgg
     5101 taaaactgtt gatgttaaag tcaaacaaaa taagcaaaca gactggtcag aagataagtt
     5161 gaataatata actttgaaaa agccgactgg tggtacgatt aaattgggag atatcgctac
     5221 gttagttaaa acaacgacac caagtaaatt gacgcaagaa caaggagatt atgcaacgac
     5281 ggtatctgct aaagtaacaa ataaagatgt gggtggcaca acacgacaag tgatgtctaa
     5341 aataaataat ttggacaaac cgaataatgt aaaggttaat atcggtggtg catcagatga
     5401 tattaacaat gcaatgacac aattagcctt tgcaatgtta gctgcaatca ttatcgtata
     5461 tttaatccta gttattacat ttaaaggtgg cctagcacca tttacaattt tattctcttt
     5521 accatttaca gttatcggtg taattattgc actattaatc acaggagaaa caatatcagt
     5581 accaagttta attggtatgc taatgttaat tggaatcgta gtaacaaatg ccattgtgtt
     5641 aatagaccgt gttattaata atgagcaaca gggcatggag atgaaagaag cattaatcga
     5701 agcaggcggt actagaatta gaccgatatt aatgacggcg attgcaacaa ttggtgcatt
     5761 agttcctttg ttatttggtc aagatagctc gattcttatt tcgaaaggat tagctgctac
     5821 agttattggt ggtttaattt catcaacttt attaacactt gtagttgtac cagtaatata
     5881 tgaaattctc tttactttaa aaaaacgatt cactaaacga tagataatat aaaagccata
     5941 aaagcggtca tgatattgac tacttttatg gctttttaaa atttgaaatt ttagacctta
     6001 tttcacgtgc gatttaatcg atgtgttaat aaaacaatac tggcattcaa catgtaatcc
     6061 gataataaaa taaggttgca attatgttat tgcatatttt gtttattact tagaataata
     6121 cgatggttaa gatgaaatat atgacaaaga taaataatga cattaaattc aagatgttta
     6181 cgccttttag atctttttta agcattttaa atcctgtcac ttcgacatta ccgactaaga
     6241 gtaataagac gataaatacc caccaattct cttttggaaa actcatcgtc gttaatgcta
     6301 agacgaataa taacaacaca cccataataa ttctcaaaat gcgagtaaat acaacttcca
     6361 ttttaaaaat actaataaaa ctaacaatta gataaagtat tgctagtaaa atcagataaa
     6421 attgcatatg ataacttcct ctccgtttca aagggttcat gtagtacagc agcgtagaaa
     6481 acataaatag cagtgttggg atagatatgc gtgtatgttg ttaatgaaac actaaatcat
     6541 aacacgacaa catcttgtga ttctagatgt aattgagcga aaaatgaagc aattcatcaa
     6601 tataacgtat tcagagcaat gctagtcaaa tttatgtaac aaaaagcatt tattgcttga
     6661 atgatagatt attaatcttg catttgagca cgaacgtttc taatttcatt ttcaatatct
     6721 ttaagttgac gaacaagtgc ggaagtatct aaattcaaag attcccgttt ttctatgtca
     6781 ccttgaagac gaacataatc atatttaagt tcggctagtt ggttttctaa agacatacta
     6841 taacactcct ttgaaatata attaagtgca tttaatgtag attatagcat gaaacagtag
     6901 acttctaaaa tatctgtgga gtatgttaaa gttataaatt atgaagatta ttgaaagaag
     6961 ggataacagt gacagaacaa tttactatga ttattttatt aattgcgctt ggttactttt
     7021 tgaagcgcat taattttata aaagcaacag atagtcaagt cattgctaca ttagttctta
     7081 atgtcacact cccatcttta gttattgtga atttaaacag tgcagaatta aaattgtcat
     7141 tctccatttt accaatttta atgattatat atggtattgt ggcgaaaatg attgctgtct
     7201 ggttttttag aaaatatgat aatcacatgc gtggatcagt aggtatgatg actggtgcta
     7261 tgaatattgg attgtttgca tatccactaa ttgaagtgat atggcctaaa acgggactta
     7321 tttattttgt tatggctgat ataggtggtg cctttgtgat gtttggaatt acttactttg
     7381 taggtagcta ctttagtgaa ggtagtgatc aattcgattt taaatattta ggtaagaaat
     7441 tattacaatc agtgccactc gttacataca ttgtaatgtt tatattgaat ataagtcaca
     7501 tccatttacc acatgtggca atcgatttct ttagcatact ttcccatgca aatatgccgt
     7561 tatcaatgat tttgctaggt gtcatgttga attttactct ggaaagaaag tatatacctg
     7621 ctactattaa atatttatgt ttgcattatg gattagcatt agttgcggga ttgttagtat
     7681 atgtgttgtt accagtatca gatgatatga ttaagacgac attgttggtc acttggatgt
     7741 tcccggtagg tgttgcaatt atttcttatg gtattcagtt taaatatcga acactgacat
     7801 ttataggtat gacgaccaat ttgacaatca ttattagtat cattattttg tacgtgtatc
     7861 aggcagtgtt tgtctagttt acacatttga aaataaaatc cattttagta ttcgtagaaa
     7921 atgagagccc cctttaccac aatatgtaaa tgtatattgt gaaaaggggg cttttattta
     7981 tgacatagtt gttcaactca tacattcaag ttgataatgc agtgcgttat gcagctatcc
     8041 acttcatatg tatggcgcat acttgatata tgaatatcat caagatttta aaagtatcgc
     8101 taattttaaa gtcttaaaac gcatgattaa tgtcatgatg tgttattgcg ttttgttttt
     8161 agagccacgt atttcctagg acatatagag gaacattcaa cgtaaaattc ccgaaaatta
     8221 attttaatca ttctataagt gaatggttat tttcagaagt gattacattt ttacgttaca
     8281 tgagtattta actttctatt tcaaattacc tagaattata gcagctatca cgataataat
     8341 aatacctgcc caaatacccg tcatctgacg acgatctttt ctttctccta aaatgaaaat
     8401 accgcctaag gttgaaacga taacaagtaa ctgtgataat gagaaacttg tagctacacc
     8461 aacttttggt tgagaataga acatgaacaa gttaccaatt ccccaaatca cacctggcaa
     8521 tagattaagt gctgttgatt taagtgatgt tttatgattc atggatagga taaagccacc
     8581 aattgccata ccgacagatt ggaagaacaa tgcatcagtt ccaccaacac caaagatgtc
     8641 accaagtaca acgaaaccta catatccaac tgtagataca attaaaatac ccattgcttt
     8701 tttaaattca ggattatctg attgacgttc atttttagct ttaagtgaag taagtgctac
     8761 accagtaact aataatatca tggcgattaa accaaagata atttgagtca ttgaagacca
     8821 ttcacctaaa aagataacgc tgaataatgt tgtaccaact aattgcatac ctgtagaaac
     8881 tggcatagta tttgatacac cgattaaact aatagattta agctgattcg cttgtccaaa
     8941 cgcccataat gcaccagaaa taagaccgac aataattaca gtaggattat tgaattttgc
     9001 gaaaccagta ataagtaaac ctaatccaac aatgagtgcg cctagcgtcg taccacgaat
     9061 ttggttgtaa ggtccaccgc cgacgaacac attaataaga acgacacttc cccagaataa
     9121 agcaggtaaa agtgcgatta agaaatcaag aaattgcaag actactcact ctcctatact
     9181 atttatgcaa gtgctcccct taattgacac ttgcatgtta ttgtaaagca ttctgcccta
     9241 atagtaaaaa acaactactt ataattttca atatttacaa aaagtagaaa aacccacaat
     9301 acatgctaat gatgtactgt agggtatttg cttggtgttt tattcaggga ttgtttgatc
     9361 ttttacgtta cgtgattcga atttgaattt aatgtactca tagttgatat ggaaacctag
     9421 accattaaat cgttcgatga gtttattatt tttattacga attttaaaat aaacttttct
     9481 aatatcaaaa ttatcaaaag catattgcaa tgcatacgca agcaactcga aagcgatacc
     9541 tttcaaacgg taatctacat gagaactgaa gtatttaatc tcggcgattg actgttgtga
     9601 atcaatttca aggtataaat aacctttaag taaaccttcg ctaacaaata aaaacaaatg
     9661 atgatggtcg tctaaactat caatgatatc ttgtggtgac tgtgcctgat atttaaatgt
     9721 agttgtgtgt aatttgctga aagcacgata aaaccctttg tgataaggaa tgatatttgg
     9781 ttggtgcata tcttcttcta atcttgtacg ggcttctatg taatagtcag tgaagttata
     9841 acttgcttga ataactttca ttaatggttt gtattgttga atgccttctt caaaagaaaa
     9901 gttaaagacg gcatcgtcag gttggttcga agtcattgct ttaaatagcg tttcaaacgt
     9961 atcgacagat aatacataat tgtcagccac gaaagggcct atgactttga atttattttt
    10021 atcgtatgca aaagacaata tgattgcttt gattgaatcg ttttctttaa tcgaaaatac
    10081 accaggtgat tcaatcgcat cggttattaa tacatctatt tcattgtact gttgaggaag
    10141 tttatataag tatgatgctg attcataatt cgaatgttca atgaacgatt ttatttgtgt
    10201 aatatcatat aactgaacta tgctcatgtc attacctccg tactttttgt tacttttatt
    10261 atatagtatt tcaactgaaa tgaaagttaa tagtgatatt aacatgttac aatacattta
    10321 acacatcatt tgcaatcatt gaatttaaat caaagattag tggaatagat gaagcacgtt
    10381 cgaaataaaa gaacgtatga gaaaggataa tttatgaaat ctttaatatt agctgaaaaa
    10441 ccatcagtcg caagagatat tgctgatgct ttacaaataa atcagaagcg taatggttac
    10501 tttgaaaata accaatatat tgtcacgtgg gcgttaggtc atctagtgac aaatgcgaca
    10561 cctgaacaat acgataaaaa tttaaaggaa tggcgattag aagaccttcc aattatacct
    10621 aaatatatga aaactgttgt tattggtaaa acaagcaaac aatttaaaac agtaaaagcg
    10681 ttaattttag ataataaagt gaaagatatt attattgcaa cagatgctgg acgagaaggt
    10741 gaactagttg caagattgat tttggataaa gttggtaaca aaaagccaat ccgtcgatta
    10801 tggattagct cagttactaa aaaagctatt caacaaggtt ttaaaaattt aaaagacggt
    10861 cgtcaatata acgatttgta ttatgcagcg ttagcgagaa gtgaggcaga ttggattgtt
    10921 gggattaatg caacgcgtgc actaacaaca aagtatgatg cacagctatc cctgggacgt
    10981 gttcagacac caacgattca attagtaaat acacgacaac aagagattaa tcagttcaaa
    11041 ccacaacaat actttacatt atcattaacg gtaaaagggt ttgattttca gctagaatca
    11101 aatcagcgat ataccaataa agaaacttta gaacagatgg ttaataattt gaaaaatgtc
    11161 gatggtaaga ttaaatctgt tgctactaaa cataagaagt cgtatccgca atcactgtac
    11221 aatttaacag atttacaaca agatatgtat agacgttata aaattggacc taaagaaaca
    11281 ttgaatacac ttcaaagctt atatgagaga cataaagtcg taacctatcc aagaacagat
    11341 tcaaactatt taacaactga tatggtagat actatgaaag aacgtattca ggcgacgatg
    11401 gcaacaacat ataaagacca agcacgccca ttaatgtcta aaacattttc atcaaaaatg
    11461 tcgatattta ataatcaaaa agtatctgat caccatgcaa ttattcctac agaagtgaga
    11521 cctgtcatgt cagacttaag taatagagaa ttaaagttat acgatatgat tgtcgagcgt
    11581 tttttagaag ctttaatgcc tccgcacgag tatgacgcga taactgtaac tttagaggtt
    11641 gcagggcaca catttgtttt gaaagagaat gtaacaactg ttttaggttt taaatctatt
    11701 agacaaggtg aatctattac agagatgcaa cagccttttt cagaaggcga tgaagtgaag
    11761 atttcaaaaa caaatattag agaacatgaa acaacacctc cagaatattt taatgaaggt
    11821 tcgttattaa aagcgatgga gaaccctcag aactttattc aattgaagga taaaaaatat
    11881 gcgcaaactt taaaacaaac aggtggtatc ggcacagttg caacaagggc cgacattatc
    11941 gataaattat ttaatatgaa tgccattgaa tcaagagacg gtaaaattaa agtaacgtca
    12001 aaaggtaaac aaatattaga attagcacca gaagaattaa cgtcgccact tttaactgca
    12061 caatgggaag aaaaattact tttaattgaa cgtggtaaat atcaggcgaa aacatttatt
    12121 aatgaaatga aagattttac gaaagatgtt gtaaatggga ttaaaaatag tgatcgtaaa
    12181 tataaacacg ataatttaac aaccacagaa tgcccaacgt gtggtaaatt catgattaaa
    12241 gttaaaacta aaaatggtca gatgcttgtg tgccaagatc catcttgtaa gacgaaaaag
    12301 aatgtacagc gcaaaacaaa tgcaagatgt ccaaactgta aaaagaaatt aacgttgttt
    12361 ggtaaaggga aagaagcggt atatcgttgt gtttgtggac attctgaaac gcaagcacat
    12421 atggatcagc gtatgaagtc taaatcctct ggtaaagtat ctcgtaaaga aatgaaaaag
    12481 tatatgaata aaaatgaagg tttagacaat aatccgttta aagatgcatt aaagaacttg
    12541 aatttataga taaaatcgaa caaagttgaa tcagaaaaac gaaaagttcg cttttggtat
    12601 tgttttttat taagaatgat attaaactat taaggtattt taaaaaaagg agcatccatt
    12661 cgtgaaaaac tatttccagt tcgataaata tggaacaaac tttaaaagag aaatcttagg
    12721 cggtatcaca actttcttat ctatggccta tattttagca gttaacccgc aagttttaag
    12781 tttagcaggt gttaaaggcg tatcagaaga tatgaaaatg gaccaaggtg ccatttttgt
    12841 agcgactgca ttagcagcat ttgtaggctc gctattcatg gggctaatag ctaaatatcc
    12901 aattgcgtta gcaccaggta tgggattaaa tgcattcttt gcatttactg ttgtgttaac
    12961 catgggtatt ccttggcaag ttggtttaac aggcgtttta ttctcaggta tattctttgc
    13021 gatattaacc gtcacagggt ttagggaagt tattattaat gcaattcctt atcaaatgaa
    13081 gatggctgtt tcagcaggta taggattatt cattacattt gtaggtttac aaagttcggg
    13141 tattatagtt aaaaatgaat ctacattagt aacattggga catttaactg atccaccagt
    13201 acttttggcg atattcggta tcgttattac agttatttta tatgcaatta aattgccggg
    13261 ttctatcttt atcggtatga tcatcacagc aatcgttggt atgttcactg gattgattca
    13321 aatgccatct ggtattgtgg gtaagattcc aagtattgaa ccaacgttcg gtgctgcatt
    13381 tgaagcgttt aaagacccaa gtcaattatt aacgatacag ttcttaattg ttattttaac
    13441 gttcttattt attgatttct ttgatacagc tggtacatta gtagctgttg ctactcaagc
    13501 aggtattatg aaagataata aattaccaag agctggtaga gcattgttct cagattcttt
    13561 agctacgatt gtaggatcag tttttggaac aactacaaca acttcttata tcgaatcaac
    13621 ttcaggtgtt gctgtaggtg ctagaacagg atttgctagt atcgtaacag gttgttgttt
    13681 cctattagcg ctgttcttta gtccgttaat cgctgttgta acaagtgcag ttacgacacc
    13741 agccttagtg gtggtaggtg tattaatggc tgcaaacttt gctgagataa actggaaaag
    13801 ttttgaagtt gcagtgccag ccttcattac aatcattatg atgcctttat catattcaat
    13861 tgcaactggt attgcgtgtg gatttatttt ctatccaatt acaatgctta tctctaaaaa
    13921 acataaagaa gtacatccga taatgtatgt attaatggta ctattcattc tgtacttcat
    13981 atttgttcat ggttaaatcc agacacaaaa aacgactgcc aatgattggt agtcgttttt
    14041 ttctttgaag taaggataaa ataagaagcg aaataacata aagcgatatt atgaatgtct
    14101 ttaaatatcc gatataaata tacttctttt aaaattgttg gacgtgtttc ttctatatta
    14161 tatacctaat taaaaaggtt ttaagaatta ttctctatgt ccatctttgt attgtgtgta
    14221 aatgacacga gcatttttat taggtatata ggttggcgct gataatacaa ctaggacaaa
    14281 tagaatcatt aggaaaaata aaatccaatg aatcaaaatt cctaagaagg ggataatagc
    14341 taaaaaacta cctactatac caaatattgg aataatcgct ataggtctaa tcgcattttg
    14401 tctattcact aataatataa tagtaagaat tagataaagt aatgcattaa agagtaaagg
    14461 ctgccatcca aaacttaaaa taaacgttgc acctaaaaaa ggaatgccat agaaaaactc
    14521 acaaagtatt aaaaagatac ctataagtgc taaagataat tttggtccat ttttcatatt
    14581 cattcaccac cgttcttatg actaattata tagaataatt ttcaatgata ataataaaat
    14641 gattcaaggt acgaatttac aataaagaag gaaataaaaa atatctcgaa aatagttgaa
    14701 ctgactaagt atataaagta aaatataaaa gtatgtgtta gacagataga aaaattatgt
    14761 gaaaagcctt gatttatcga tgtttttcgt gtaatataac taacgttgga ctttaagaaa
    14821 agcgatgaag cgaaaggtta ctgacacacc cggccgcttt gccatggcgc tgtgtaagat
    14881 agttttcgtg gagaagtcta tcactaaatg tagacgaata aggagggaaa attatggcaa
    14941 aacaaaaaat cagaatcaga ttaaaagctt atgatcaccg cgtaattgat caatcagcag
    15001 agaagattgt agaaacagcg aaacgttctg gtgcagatgt ttctggacca attccgttac
    15061 caactgagaa atcagtttac acaatcatcc gtgccgtgca taagtataaa gattcacgtg
    15121 aacaattcga acaacgtaca cacaaacgtt taatcgatat tgtaaaccca acaccaaaaa
    15181 cagttgacgc tttaatgggc ttaaacttac catctggtgt agacatcgaa atcaaattat
    15241 aatagacaat tttaggaggt ggactttcga tgaccaaagg aatcttagga agaaaaattg
    15301 ggatgacaca agtattcgga gaaaacggtg aattaatccc tgtaacagta gtagaagcta
    15361 aagaaaatgt tgtattacaa aagaaaactg tagaagttga tggatacaac gcaatccaag
    15421 ttggatttga agacaaaaaa gcatacaaaa aagatgcaaa atctaataaa tatgctaata
    15481 aaccagctga aggtcacgct aaaaaagctg acgcagcacc taagcgcttc attcgtgaat
    15541 tccgcaatgt agacgtggat gcttacgaag taggtcaaga agtctcagta gatacttttg
    15601 tagctggcga cgttattgac gtaacaggcg tatcaaaagg taaaggtttc caaggtgcaa
    15661 ttaaacgcca cggacaatct cgtggaccta tgtcacacgg ttctcatttc cacagagcac
    15721 caggttctgt aggtatggct tcagatgctt ctagagtatt taaaggccaa aaaatgccag
    15781 gacgtatggg tggaaacact gtaactgttc aaaacttaga agtagttcaa gttgacacag
    15841 aaaacaaagt tatcttagta aaaggtaacg tacctggacc taaaaaaggt ttagtagaaa
    15901 tcagaacttc aattaaaaaa ggtaataaat aataagaagt gaaaggagga aatgcataat
    15961 ggctaattat gatgttttaa aattagacgg aactaaatca ggttcaatcg aattaagcga
    16021 tgcagtattc ggtattgagc caaataatag cgttttattc gaagctatta atttacaacg
    16081 tgcttcatta cgtcaaggta cgcatgctgt taagaatcgt tcagcagtaa gcggtggcgg
    16141 acgtaaacca tggaagcaaa aaggaacagg tcgtgctcgt caaggtacaa tccgtgctcc
    16201 acaatggcgt ggcggtggta tcgtattcgg accaactcca agaagttatg catacaaaat
    16261 gcctaagaaa atgcgtcgtt tagctttacg ctcagcatta tctttcaaag ctcaagagaa
    16321 tggcttaact gtagttgacg cattcaactt cgaagctcca aaaactaaag aattcaaaaa
    16381 tgtattatct acattagaac aacctaaaaa agtattagta gttactgaaa acgaagatgt
    16441 aaatgttgaa ttatcagcac gcaacatccc tggcgttcaa gtgacaactg ctcaaggttt
    16501 aaatgtttta gatatcacta atgctgacag cttagtaatt actgaagctg ctgctaaaaa
    16561 agttgaggag gtgctcggat aatggaagca agagatattc ttaagcgccc cgtaatcact
    16621 gagaaatctt ctgaagcaat ggctgaagac aaatacactt tcgacgttga tactcgtgtt
    16681 aacaaaacac aagtaaaaat ggcagttgaa gaaatcttca acgtaaaagt tgcaagtgtt
    16741 aatatcatga attacaaacc taagaaaaaa cgtatgggcc gttaccaagg ctatacaaac
    16801 aaaagaagaa aagcgattgt aactcttaaa gaaggatcaa tcgacttatt taactaataa
    16861 aacaattacc attaaggagg taagcgacaa tggctattaa aaagtataag ccaataacaa
    16921 atggtcgtcg taatatgact tcgttagatt tcgcagaaat cacgaaaact acacctgaaa
    16981 agtcattatt aaaaccgcta ccgaaaaaag cgggacgtaa caaccaaggt aaattgactg
    17041 taagacacca tggtggtgga cacaaacgtc aataccgtgt tatcgatttc aaacgtaaca
    17101 aagatggtat caatgcaaaa gttgattcta ttcaatatga tccaaaccgc tcagcaaaca
    17161 tcgctttagt tgtatatgca gacggtgaaa aacgatatat cattgctcct aaaggattag
    17221 aagtaggtca aatcgttgaa agtggtgctg aagctgacat caaagttggt aacgcattac
    17281 cattacaaaa cattccagtt ggtacagtag tacacaacat cgagcttaaa cctggtaaag
    17341 gtggacaaat cgctcgttca gctggtgcaa gtgctcaagt acttggtaaa gaaggtaaat
    17401 atgtattaat cagattaaga tctggtgaag ttcgtatgat cttatctact tgccgtgcta
    17461 caatcggtca agttggtaac ctacaacacg aattagttaa cgttggtaaa gccggacgtt
    17521 caagatggaa aggtatccgt ccaacagttc gtggttctgt aatgaaccct aacgatcacc
    17581 cacacggtgg tggtgaaggt cgtgctccta tcggtagacc atctccaatg tcaccatggg
    17641 gtaaacctac gcttggtaag aaaactcgtc gtggtaaaaa atcatcagac aaacttatcg
    17701 ttcgtggacg taagaaaaaa taatataatc aacttatttg ggtgtgcggc ttaaagctgc
    17761 acgcacataa taagaaggga ggcgcccaaa tggctcgtag tattaaaaaa ggacctttcg
    17821 tcgatgagca tttaatgaaa aaagttgaag ctcaagaagg aagcgaaaag aaacaagtaa
    17881 tcaaaacatg gtcacgtcgt tctacaattt tccctaattt catcggacat acttttgcag
    17941 tatacgacgg acgtaaacac gtacctgtat atgtaactga agatatggta ggtcataaat
    18001 taggtgagtt tgctcctact cgtacattca aaggacacgt tgcagacgac aagaaaacaa
    18061 gaagataata tctattaagt agaggaggac atcctaatgg aagcaaaagc ggttgctaga
    18121 acaataagaa tcgcacctcg taaagtaaga ctagttcttg acttaatcag aggtaaaaat
    18181 gctgctgaag ctattgcaat tttaaaatta acaaacaaag cttcatcacc agtaattgaa
    18241 aaagtattaa tgtccgcttt agctaatgct gaacataact atgacatgaa cacagatgaa
    18301 ttagtagtta aagaagcata tgctaacgaa ggaccaacat taaaacgttt ccgtccacgt
    18361 gcgcaaggtc gtgcaagtgc gattaacaaa cgtacaagcc acattacaat cgtcgtaagt
    18421 gacggtaaag aagaagctaa agaagcttaa ttaactttta aggagggaat actgtgggtc
    18481 aaaaaattaa tccaatcgga cttcgtgttg gtattatccg tgattgggaa gctaaatggt
    18541 atgctgaaaa agacttcgct tcacttttac acgaagattt aaaaatccgt aaatttattg
    18601 ataatgaatt aaaagaagca tcagtttctc acgtagagat tgaacgtgct gcaaaccgta
    18661 tcaacattgc aattcatact ggtaaacctg gtatggtaat tggtaaaggc ggttcagaaa
    18721 tcgaaaaatt acgcaacaaa ttaaatgcgt taactgataa aaaagtacac atcaacgtaa
    18781 ttgaaatcaa aaaagttgat cttgacgctc gtttagtagc tgaaaacatc gcacgtcaat
    18841 tagaaaaccg tgcttcattc cgtcgtgtac aaaaacaagc aatcactaga gctatgaaac
    18901 ttggtgctaa aggtatcaaa actcaagtat ctggtcgttt aggcggagct gacatcgctc
    18961 gtgctgaaca atattcagaa ggaactgttc cacttcatac gttacgtgct gacatcgatt
    19021 atgcacacgc tgaagctgac actacttacg gtaaattagg cgttaaagta tggatttatc
    19081 gtggagaagt tcttcctact aagaacacta gtggaggagg aaaataataa tgttactacc
    19141 aaaacgtgta aaatatcgtc gtcaacatcg tcctaaaaca actggtcgtt ctaaaggcgg
    19201 taactacgta acatttggtg agtttggttt acaagctaca acaacgtctt ggatcacatc
    19261 tcgtcaaatc gaatctgctc gtatagcaat gacacgttac atgaaacgtg gcgggaaagt
    19321 ttggattaaa atcttcccac atacaccata tactaaaaaa cctttagaag tacgtatggg
    19381 tgctggtaaa ggtgcggttg aaggctggat cgcagttgtt aaaccaggta gaattttatt
    19441 cgaagttgct ggcgtttctg aagaagttgc gcgtgaagca ctacgtttag caagtcacaa
    19501 acttccagta aaaactaagt ttgtaaaacg tgaggaattg ggtggtgaaa caaatgaaag
    19561 ctaaggaaat tagagactta accacttcag aaatcgaaga acaaatcaaa tcttcaaaag
    19621 aagagctttt taacctacgc tttcagttag ctacaggtca attagaagaa actgcacgta
    19681 ttcgtacagt aagaaaaacg attgcacgtc taaaaactgt tgctcgtgaa agagaaattg
    19741 aacaaagtaa ggctaatcaa taattaagta agaggaggtt acaaaagtga gcgaaagaaa
    19801 cgatcgtaaa gtttatgtag gtaaagttgt ttcagacaaa atggacaaga ctattacagt
    19861 acttgttgaa acttacaaaa cacacaaatt atacggtaaa cgagtaaaat actctaaaaa
    19921 atacaaaact catgatgaaa acaattcagc taaattagga gacattgtta aaattcaaga
    19981 aactcgtcct ttatcagcaa caaaacgttt tcgtttagta gagattgttg aagagtcagt
    20041 aattatttaa tacaagttta gagataaggg aggtttaact aatgatccaa caagaaacac
    20101 gcttgaaagt agcagacaac tctggtgctc gtgaagttct tacaatcaaa gtattaggtg
    20161 gatctggtcg taaaacagca aacatcggcg atgttatcgt atgtactgtt aaaaatgcaa
    20221 caccaggtgg cgttgttaaa aaaggtgacg ttgtcaaagc tgtaatcgta cgtactaagt
    20281 caggtgttcg tcgtaatgac ggttcataca tcaaatttga tgaaaatgca tgtgttatca
    20341 tccgtgatga caaaggccca cgtggtactc gtatcttcgg acctgttgct cgtgaattac
    20401 gtgaaggtaa cttcatgaaa atcgtatcat tagcaccaga agtactttaa ttaataacaa
    20461 ccaatacaaa taaggaggtg cccacatgca tatcaaaaaa ggtgacaacg ttaaagttat
    20521 cgcaggtaaa gacaaaggta aagaaggtaa agtaattgct actctaccta aaaaagaccg
    20581 tgtcgttgtg gaaggtgtta acattatgaa aaaacaccaa aaaccaactc aattaaatcc
    20641 tgaaggtgga atcttagaaa cagaggcagc aatccatgtt tctaatgtac aattattgga
    20701 ccctaaaaca aacgaaccaa ctcgtgtagg ttacaaattt gttgatggta aaaaagttcg
    20761 tatcgctaaa aaatctggcg aagaaattaa atctaataat taataacaag ttgaaaggag
    20821 gatcccactt tgaaccgttt aaaagaaaag tttaacactg aagttactga aaacttaatg
    20881 aaaaaattca attatagttc agtaatggaa gtaccaaaaa tagataaaat cgttgtgaac
    20941 atgggtgtag gtgacgcagt acaaaattct aaagtattag acaatgctgt tgaagaatta
    21001 gaattgatca ctggtcaaaa accattagta actaaagcta aaaaatcaat cgcgactttc
    21061 cgtttacgtg aaggtatgcc aatcggtgcg aaagtaacac ttcgcggtga aagaatgtat
    21121 gaattcttag acaaattaat ttcagtatca ttaccacgtg tacgtgactt ccaaggtgtt
    21181 tctaaaaaag catttgacgg acgcggtaac tacactttag gtgttaaaga acaattaatt
    21241 ttcccagaaa tcgactatga taaagtaagt aaagttagag gaatggatat tgttatcgta
    21301 acgactgcta acactgatga agaagctcgt gaattgttag ctaacttcgg tatgccattc
    21361 cgtaaataat tatttaaagg aggctaatta agtggctaaa acttcaatgg ttgctaagca
    21421 acaaaaaaaa caaaaatatg cagttcgtga atacactcgt tgtgaacgtt gtggccgtcc
    21481 acattctgta tatcgtaaat ttaaattatg ccgtatttgt ttccgtgaat tagcttacaa
    21541 aggccaaatc cctggcgttc gtaaagctag ctggtaataa aaaagagtct gaaaggaggc
    21601 aacaatcaat gacaatgaca gatccaatcg cagatatgct tactcgtgta agaaacgcaa
    21661 acatggtgcg tcacgagaag ttagaattac ctgcatcaaa tattaaaaaa gaaattgctg
    21721 aaatcttaaa gagtgaaggt ttcattaaaa atgttgaata cgtagaagat gataaacaag
    21781 gtgtacttcg tttattctta aaatatggtc aaaacgatga gcgtgttatc acaggattaa
    21841 aacgtatttc aaaaccaggt ttacgtgttt atgcaaaagc tagcgaaatg cctaaagtat
    21901 taaatggttt aggtattgca ttagtatcaa cttctgaagg tgtaatcact gacaaagaag
    21961 caagaaaacg taatgttggt ggagaaatta tcgcatacgt ttggtaataa aaaataagga
    22021 ggtgccataa catgagtcgt gttggtaaga aaattattga catccctagt gacgtaacag
    22081 taacttttga tggaaatcat gtaactgtta aaggtcctaa aggtgaatta tcaagaactt
    22141 taaatgaaag aatgacattc aaacaagaag aaaacacaat tgaagttgta agaccatctg
    22201 attctaaaga agatagaaca aaccatggta caactcgtgc tttattaaac aatatggtac
    22261 aaggtgtttc tcaaggatac gtaaaagtac ttgaacttgt tggtgtaggt taccgtgctc
    22321 aaatgcaagg taaagactta atccttaacg ttggttattc tcacccagta gaaattaaag
    22381 ctgaagaaaa cattactttc tcagttgaga aaaacacagt cgttaaagtt gaaggtattt
    22441 caaaagaaca agttggagca ttagcatcta acatccgttc agtaagacct ccagagcctt
    22501 acaaaggtaa aggtattcgt taccaaggtg aatacgttcg ccgtaaagaa ggtaaaactg
    22561 gtaaataata gataaactct aaagaaagga gtattgaaat gatcagtaaa attgataaaa
    22621 ataaagtgcg tttaaaaaga catgctcgtg ttcgtactaa cttatcaggt acagctgaaa
    22681 agccacgttt aaacgtatat cgttcaaaca agcatatcta cgctcaaatt attgatgata
    22741 ataaaggcgt aacattagct caagcttctt caaaagacag cgacattgct actacagcaa
    22801 ctaaagttga attagcaact aaagtcggtg aagcaattgc taaaaaagct gctgacaaag
    22861 gcattaaaga aatcgtattt gaccgtggag gatatttata tcacggacgt gttaaagcat
    22921 tagctgaagc agcaagagaa agcggattag aattttaatt taaaggaggg acaaatacat
    22981 ggctcgtaga gaagaagaga cgaaagaatt tgaagaacgc gttgttacaa tcaaccgtgt
    23041 agcaaaagtt gtaaaaggtg gtcgtcgttt ccgtttcact gcattagttg tagttggaga
    23101 caaaaatggt cgtgtaggtt tcggtactgg taaagctcaa gaggtaccag aagcaatcaa
    23161 aaaagctgtt gaagcagcta aaaaagattt agtagttgtt ccacgtgttg aaggtacaac
    23221 tccacacaca attactggcc gttacggttc aggaagcgta tttatgaaac cggctgcacc
    23281 tggtacagga gttatcgctg gtggtcctgt tcgtgccgta cttgaattag caggtatcac
    23341 tgatatctta agtaaatcat taggatcaaa cacaccaatc aacatggttc gtgctacaat
    23401 cgatggttta caaaacctta aaaatgctga agatgttgcg aaattacgtg gcaaaacagt
    23461 agaagaatta tacaattaag gagggaaaac tagttatggc taaattacaa attaccctca
    23521 ctcgtagtgt tattggtcgt cctgaaacac aacgtaaaac tgttgaagct ttaggtctta
    23581 aaaagactaa cagttcagta gttgttgaag ataaccctgc tattcgtggg caaatcaaca
    23641 aagttaagca cttagtaaca gtagaagaaa aataatcaag gaggtgccga aatgaaatta
    23701 catgagttaa aaccggcaga aggttcacgt aaagaacgca atcgtgttgg acgtggtgtt
    23761 gcgacaggta acggtaaaac aagtggtcgc ggacacaaag gtcaaaaagc tcgttcaggc
    23821 ggtggtgtaa gaccaggatt tgaaggtggt caattaccat tattccgtcg tttaccaaaa
    23881 cgtggtttta ctaacataaa tcgtaaagaa tatgctattg ttaacttaga ccaacttaat
    23941 aaatttgaag atggtactga agtaactcca gctttattag tagaatctgg tgttgttaag
    24001 aatgaaaaat ctggtatcaa aatactaggt aatggttcac ttgataagaa attgacagtg
    24061 aaagctcata aattctcagc ttcagcagca gaagctattg atgctaaagg tggagcacac
    24121 gaggtgatct aatgattcaa acccttgtga acttctttag aacaaaagag gttcgtaaca
    24181 agattttctt cacactagca atgttagtaa tttttaaaat agggacttat ataccagctc
    24241 caggagtaaa tcctgcagct tttgataatc cccaaggttc tcaaggtgcc actgagttat
    24301 taaatacttt tggtggcgga gccttgaaac gattttctat ttttgcaatg ggtattgtac
    24361 cctacatcac tgcatcaatc gtaatgcaat tattacaaat ggatattgtc cctaaattct
    24421 cagaatgggc aaaacaaggt gaagtaggta gaagaaagtt aaataacgtt actcgttatt
    24481 tagcaatttc tttagcattt atccaatcta taggtatggc attccaattt aataattatc
    24541 tcaaaggtgc gctgattatc aatcagtcaa ttatgagtta tttattaata gcactagttt
    24601 tgacagcagg aactgctttc ttaatatggc ttggtgatca aatcactcag ttcggtgttg
    24661 gtaatggtat ttctattatc atatttgcgg gtattttatc aacgttgcca gccagtctaa
    24721 ttcaatttgg acaaactgcg ttcgttgggc aagaagatac atcattagca tggttgaaag
    24781 tattaggact tttagtgtca ctgatactat taactgtagg tgctatttat gtacttgaag
    24841 ccgttcgtaa aattccgatt caatatgcta agaaacaaac tgctcaaaga ttaggttcac
    24901 aggcaactta tctaccttta aaagtgaact cagccggtgt tattcctgta atctttgcaa
    24961 tggcgttctt cttactgcct agaacattaa cgttgttcta tccagataaa gaatgggcac
    25021 aaaacattgc gaatgctgca aatccttcaa gtaatgttgg tatggttgta tatattgtac
    25081 taattatact attcacatac ttctatgcat ttgtacaagt taatccggaa aaaatggctg
    25141 ataaccttaa gaaacaaggt agctatgttc caggtattag acctggtgaa caaaccaaaa
    25201 aatatattac taaagtttta tatcgtttaa cttttgtagg ttctatcttc ttagccgtta
    25261 tttcaattct accgatatta gcaactaagt ttatgggatt accacaatca attcagattg
    25321 gtggtacaag tttacttatc gttattggtg tagcgattga aacaatgaaa tctttagaag
    25381 ctcaagtatc tcaaaaagaa tataaaggct ttggtggtag ataattttag gagggcactt
    25441 atgaatatca ttttgatggg tttacctggc gcaggtaaag gaactcaagc aagtgaaatt
    25501 gtcaagaaat tcccaatacc ccacatttca actggtgaca tgttcagaaa agctataaaa
    25561 gaagaaactg aattaggtaa agaagctaag tcttatatgg accgtggcga attagttcct
    25621 gatgaagtga ctgtaggtat cgttaaggaa agaatttctg aagacgatgc aaaaaaaggc
    25681 tttttattag atggcttccc aagaacaatc gagcaagctg aggcattaaa taatattatg
    25741 tctgagcttg acagaaacat tgatgctgtc atcaatatcg aagttccgga agaagaatta
    25801 atgaaccgtc ttacaggtcg tcgaatctgt gagtcatgtg gtacaacgta tcatcttgta
    25861 tttaatcctc cgaaggtcga aggtatttgt gatatcgatg gtggtaaatt gtatcaacga
    25921 gaagatgata atcctgaaac ggtagctaat cgtttgagtg ttaatattaa acaatctaag
    25981 cctattttag atttctatga tcaaaaaggt gtattgaaaa atattgatgg ttcaaaagat
    26041 attagcgacg ttaccaaaga tgtcattgat attttagatc atttgtaata gatcaacttt
    26101 tagcggtcta ttttaagtga ggcatgattt aatcatatta gatagatgag agtggcaatt
    26161 aacgataatt aattcccgta atttttgttt aacttataaa attaatcact gatagattcg
    26221 taacaataaa gttgtctatt gacgattacc ttactattgc aaaaaggggg aagttaatca
    26281 atggctaaac aagatgtaat tgaattagaa ggtactgtat tagatacttt accgaacgca
    26341 atgtttaaag tagaattaga aaatggtcat gagattttag ctcacgtaag tggtaaaatc
    26401 agaatgaatt acattcgtat tctacctggc gacaaagtaa ctgttgagat gtctccgtac
    26461 gatttaacac gcggaagaat tacttatcgt tataaataat cgtcactcca taatataggg
    26521 aggtataaaa atgaaagtaa gaccatcagt aaaacctatt tgcgaaaaat gtaaagtcat
    26581 taaacgtaaa ggtaaagtaa tggtaatttg tgaaaatcca aaacacaaac aaagacaagg
    26641 ttaataaaag agaggtgtaa attaatatgg cacgtattgc aggagtagat attccacgtg
    26701 aaaaacgcgt agttatctca ttaacttata tatacggtat cggtacgtca actgctcaaa
    26761 aaattcttga agaagctaac gtatcagctg atactcgtgt gaaagattta actgatgacg
    26821 aattaggtcg catccgtgaa gttgtagacg gttataaagt cgaaggtgac ttacgtcgtg
    26881 aaactaactt aaatatcaaa cgtttaatgg aaatttcatc ataccgtggt atccgtcacc
    26941 gtcgtggttt accagttcgt ggtcaaaaaa cgaaaaacaa cgcgcgtact cgtaaaggac
    27001 cagttaaaac ggtagctaac aagaaaaaat aataggtaaa ggaggcaaat tttaaatggc
    27061 acgtaaacaa gtatctcgta aacgtagagt gaaaaagaat attgaaaatg gtgtagcaca
    27121 catccgttca acattcaaca acactattgt aactatcact gatgagttcg gtaatgcttt
    27181 atcatggtca tcagctggtg cattaggatt caaaggatct aaaaaatcaa caccatttgc
    27241 agcacaaatg gcttctgaaa ctgcatctaa atcagctatg gagcatggtt taaaaacagt
    27301 tgaagtaaca gttaaaggac ctggtccagg tcgtgaatca gctattcgtg cattacaatc
    27361 tgcaggttta gaagtaactg cgatcagaga cgttactcca gtacctcata acggttgtcg
    27421 tccaccaaaa cgtcgtcgtg tataatttat gatggtattg ttacaggtca ctgagcaaac
    27481 attttaaatt aagtcgacgt atataaggag gatatttaaa tgatagaaat cgaaaaacct
    27541 agaattgaga caattgaaat tagtgaagat gctaaattcg gtaagttcgt tgttgaacca
    27601 ctagaacgtg gctacggtac tacactagga aactccttac gtcgtatcct actatcttca
    27661 ttaccaggtg cagccgttaa gtatattgaa attgagggag ttttacatga attctcagca
    27721 gtagacaatg tagttgaaga tgtttctaca attattatga acattaaaca attagcattg
    27781 aaaatttact ctgaagaaga taaaacttta gaaattgatg tacgtgatga aggcgaagta
    27841 acagcaagcg acattacaca tgatagtgat gttgaaattt taaacccaga gcttaaaatt
    27901 gcaacagtat ctaaaggtgg tcacttaaaa attcgtctag ttgctaacaa gggtagaggt
    27961 tacgcattag cagaacaaaa taatactagt gatttaccaa ttggtgtaat ccctgttgat
    28021 tcattgtatt cacctgttga acgtgtgaac tatactgttg aaaatacacg tgtaggtcaa
    28081 agcagtgatt ttgataaatt aacattggat gtttggacta atggttcaat cacaccacaa
    28141 gaatcagttt cattagcagc aaaaataatg actgaacact tgaatatctt cgttggtctt
    28201 actgatgaag cgcaaaacgc tgaaatcatg attgaaaaag aagaagatca aaaagaaaaa
    28261 gtattagaaa tgtctattga agaattagac ttatctgtac gttcatataa ctgcttaaaa
    28321 cgcgcaggaa tcaattctgt tcaagagtta gctgacaaat ctgaagctga catgatgaaa
    28381 gtgcgtaatt taggtcgtaa atctttagaa gaagttaaat acaaattaga agatttagga
    28441 ttaggattaa gaaaagaaga ttgataaagg aggttaactc atgggttaca gaaaattagg
    28501 tcgtacttct gatcaacgta aagctatgtt acgtgactta gctacatcac ttattattag
    28561 tgaacgtatt gaaactacag aagctcgtgc aaaagaagtt cgcagtgttg ttgagaaatt
    28621 aatcacttta ggtaaaaaag gagatttagc ttctcgtcgt aatgcagcta aaactttacg
    28681 taatgttgaa atcttaaacg aagatgaaac tacacaaact gcacttcaaa aattatttgg
    28741 tgaaatcgca gagcgttaca cagaacgtca aggtggttac actcgtatcc ttaaacaagg
    28801 ccctcgtcgc ggtgacggtg ctgaatcagt aattatcgaa ttagtataag aatttatata
    28861 aatacactaa ctacttttat ataaagcaaa tgagtgcaac gataatgttt gccacataca
    28921 aatattgtct agctcagagt accccatcaa ctaaataaag atttaaagcg tgaactcaac
    28981 aaacttatga tggggtgcgc gcttttttat tgaataaaat atcggactta tgaacagaaa
    29041 tgtttttaag tccgatttct ttatatttac ttttttgagt gcgaatctaa aaatatgatg
    29101 aagctagttt gatcaatttc actagaactg aatgaagtga aagtattatc gttgaatcaa
    29161 acgcgaccac ccctatatta gtaaatcatt tattttatta acgtttatac ataattgtta
    29221 gagaggaata aaagtcctta tcattagttg ttaaacaaca ctttaaagac tagaaatgag
    29281 tcagaaaata ctagtaatat atagagaacc cagtcaatgt catatacagc gatttataaa
    29341 tattgtaaaa tatgatagat acacgaatcg taaaggagag agacaagtgg aggataagaa
    29401 ttcagttatt gtatttaaaa atgtttcatt tcaatatcaa agtgatgcat ccttcacatt
    29461 gaaagatgtt tcttttaata tacctaaagg tcagtggaca tctattgttg gtcataacgg
    29521 ttctggaaaa tctacaattg ccaagttaat gattggcata gagaaagtta aatctggaga
    29581 aattttttat aataatcaag ctataactga tgataatttt gaaaagttaa gaaaagacat
    29641 aggaattgta tttcagaatc cggataatca atttgttggt tcaattgtaa aatacgatgt
    29701 ggcatttgga ctcgaaaatc atgcggttcc atatgacgaa atgcatagaa gagtcagcga
    29761 agcacttaaa caagttgata tgttagaacg tgcagattat gaacctaatg cattatcggg
    29821 gggacagaag cagcgtgtgg ctatagcaag tgtattagca cttaacccct ctgtcattat
    29881 attagatgag gcgacttcta tgttagatcc tgatgcacgt caaaatttat tggatttagt
    29941 gagaaaagtt aaatcagaac ataatattac aatcatttct attacgcatg atttatctga
    30001 ggcgatggaa gcagatcatg ttatcgttat gaataaaggg actgtctata aagaaggcac
    30061 agcgactgaa attttcgaca atgcagaaga gttaacaaga ataggtctag atttgccatt
    30121 cccaatcaaa ataaatcaaa tgctgggaca ccaaacatca ttcttaactt atgaagggct
    30181 ggtggatcaa ctatgactat acggtttgac aatgtaagtt atacctatca aaaagggaca
    30241 ccatatcagc atcaagctat tcatgatgtt aatacagaat ttgaacaagg taaatattac
    30301 gccatcgttg gacaaacggg tagtggtaaa tcaacgttga tacaaaatat taatgcgctg
    30361 ttaaagccga ctactgggac agttacagtt gatgacatta ctattacaca taagaccaaa
    30421 gataaatata ttagacctgt aagaaaaaga attggaatgg tatttcaatt tcccgaatct
    30481 caattatttg aggacacagt agagcgtgaa atgatatttg gacctaaaaa ctttaaaatg
    30541 aatttagatg aagccaaaaa ctatgcccat cgtctgttga tggatttagg cttttcaaga
    30601 gatgtaatgt cacaatcacc atttcaaatg tcaggtggac aaatgcgtaa aatagcgatt
    30661 gtatcgatat tggcaatgaa tcctgatatt atcgtggttg atgaacctac agcaggactt
    30721 gatccacaaa gtaaacgaca agtaatgaga ttactaaagt cactacaaac agatgaaaat
    30781 aaggcaatta tcctaatttc acatgatatg aatgaagtcg cgcgttatgc agatgaagtc
    30841 atcgttatga aagaaggtag tatagtgtcg caaacatcac caaaagagct cttcaaagat
    30901 aaaaaaaaat tggcggattg gcatattggt ttgccagaaa ttgttcagtt acaatatgac
    30961 tttgaacaaa aatatcaaac aaaattaaaa gatattgcct taactgaaga agcatttgta
    31021 agcttgtata aggagtggca acatgaaaaa taaattgatt atagggcgat atttaccaat
    31081 taattctttt gttcatcatc ttgatccaag agcaaagtta atgtttgttt ttctttttat
    31141 tatattaata tttttctgtc attcaccatt aacatatctt tgggtgtttg cacttatctt
    31201 attcattatg agattagcaa aaattcaatt atggttccta atcaaaggtt taacaccaat
    31261 atttttcttt ttaatcttca cattaatgat gcatatattt ttaactaaag gtggatatgt
    31321 attagttgaa tggcatggca tcacgattga aactaatggt attttagaag ggctatatat
    31381 atcactgcgt ttaattggga ttgtaatgat tgcaacaatt atgacactat ctacaagtcc
    31441 aattgattta acagatgcgt ttgaaagatt acttgcacca ctaaagatgt ttaaattacc
    31501 agttcatcaa ttaagtatga taatgtccat tgcgttacga ttcatcccga cgttaatgga
    31561 tgagttagat aaaatcattt tggcgcaaaa gtcgcgtggt tctgaaataa gttcagggaa
    31621 cattgcaaca cgtattaaat catttattcc attactagtg ccactattca tctcagcttt
    31681 ccaacgcgcc gaagaattag cggtcgcaat ggaagttagg ggttatgatg ccaatgttaa
    31741 gagaacgagc tacagacagc ttaaatggca attgagagat acgatatctt taaccatgat
    31801 tatcccaatt gcaattattt tattcgtatt aaaatattca ggagtgtaaa ttcatgcgta
    31861 tattagtaga aattgcgtat caaggaaata attttctagg ttttcaaatt caacaaaatg
    31921 gtcgaacggt acagcaacaa tttgaaaagc tattacaacg catgcataaa agacatgtga
    31981 gaatccatcc ttcaagtagg actgatagag gtgtacatgc gatacagcaa tactttcatt
    32041 tcgacacaga gctgaatata cctatgtcac aatggcaata tgcaatgaac cgaacgttac
    32101 ctgatgatat ttatgtcaac aatgtagtta cagtcgatga cgattttcat tgtcgttatg
    32161 attgtgtagg taaacgttat cgctacaaag tatatcaagc acaacaccgc gatccatttc
    32221 aaagtggttt gaaaacattt attcctgaaa cgttggattt aggtaaaatg aacagagcgg
    32281 cgcaacaatt cataggtaca catgatttta cgggattttg ttcgcaaaaa actgaagtag
    32341 aaagcaaagt tagaacatta taccaaagtg aaatcgtaaa gacagatgat ggctttgatt
    32401 atattgtcac tggatctgga tttttatata acatggtgcg tgtcttagtt gcatttttaa
    32461 tagaagttgg aaaagggcga catgaagtat ctgatgtacc aaaacttctt gaatcaaaga
    32521 atcgtaagaa tgtacctttc actgcaccgg ctgaaggatt atatttagag aaaatttatt
    32581 tagatgaaaa tgagttatta aaagactttg gtaacgatat caaaatacat cgtaaaaaat
    32641 cattacaaaa tgactgattg ccattgacaa aaagcttgtg aaattataag attattaacg
    32701 gtattgtttt atatccaccc cacgataagc cccggaaact tattgtgtta caagatatat
    32761 aagcagaaac gaacaacagt taacaaaata aatgaaatta aacgttttaa aaatgaaaca
    32821 aatgaaatca tctattaggt tatgaaactg tttatagctt gaatagaagc atttattttt
    32881 taggaggaca attattatgc gtcaaacatt tatggcaaat gaatcaaaca ttgagcgcaa
    32941 atggtatgtt atcgatgctg aaggccaaac attaggtcgt ttatcatcag aagtagcatc
    33001 tatcttacgc ggtaaaaata aagtaactta cacaccacac gttgatactg gtgattatgt
    33061 aatcgttatt aatgcatcaa aaatcgaatt tactggtaac aaagaaactg acaaagttta
    33121 ctaccgtcac tcaaatcacc caggtggtat caaatcaatc actgctggtg aattaagaag
    33181 aactaaccca gaacgtttaa ttgaaaactc aattaaaggt atgttaccaa gcactcgttt
    33241 aggcgaaaaa caaggtaaaa aattatttgt atatggtggc gctgaacatc cacacgctgc
    33301 acaacaacca gaaaactacg aattacgtgg ttaattagaa ggaggaaatg actttggcac
    33361 aagttgaata tagaggcaca ggccgtcgta aaaactcagt agcacgtgta cgtttagtac
    33421 caggtgaagg taacatcaca gttaataacc gtgacgtacg cgaatactta ccattcgaat
    33481 cattaatttt agacttaaac caaccatttg atgtaactga aactaaaggt aactatgatg
    33541 ttttagttaa cgttcatggt ggtggtttca ctggacaagc tcaagctatc cgtcacggaa
    33601 tcgctcgtgc attattagaa gcagatcctg aatacagagg ttctttaaaa cgcgctggat
    33661 tacttactcg tgacccacgt atgaaagaac gtaaaaaacc aggtcttaaa gcagctcgtc
    33721 gttcacctca attctcaaaa cgttaattgt cggacgatat atacaaaaca cctcgatatt
    33781 atgtcgaggt gtttttttgc gtttttgcgg cgaatatgga atgtgtagaa tataaatgaa
    33841 tttttacctt cccaccataa aagatgaaga accatgaaat gtggagaaca ataaatagtt
    33901 gatattctgt tatttttttg gaagtgaagt gatttgaata ctttactcta aacgataaaa
    33961 ggttaaaaaa acaacaaaaa gaaaatatta ctgtcataat tggaaataca ccgtattctg
    34021 ctggtcaatc tagtgtaaat aatgataata aaaaaatgaa tatgaaaaat taaatgcaaa
    34081 aatagcaaat acctatgcaa atttttcaac agcaaattta aaaaaatcat tatatgattc
    34141 gtatatacag gcttttcgat gggcaactga tagagttgga aaaaaggtat tataggattt
    34201 gtaacaaatg gatcatttat tgatagtcaa agcactgatg gatttagaaa agttttatac
    34261 gatgaattta actatctgta cataattaat ttgagaggcg accaacgaac acagggtgaa
    34321 aaatcaagaa aagaaggtgg aaaaatattt ggatcaggaa gtagagcacc gatagctata
    34381 tctattttag ttaaagatgg aagttacaat cacgatatct attataatga cattggtgaa
    34441 tatttaacta gagagcaaaa attagatact ttaatgaaac atcaatcaat tgtgaatttg
    34501 aaaagtttga atgttttacc cgataaaaac aatgattgga ttaatcaacg agacataaac
    34561 tatgagaatt atttaccaat gtatgattcc aaggatattg aaaattctat atatttagat
    34621 caatttaatg gagtaaattc agcaagagat aattgggtaa caaatttttc gaatgaaaaa
    34681 gcattagtaa atgcaaaatt acttgtagat aactataatt ctgaaataga tagattaatt
    34741 gatatcttag attcaagaga acgtataaat ttagtgaaca aagatgaaac atttattagt
    34801 tggacacgag gacttaccca aaaattttct aaaggaaaaa atatttcaat taatccagag
    34861 agaattgtta aatttatgca tagaccattt acaaaaaaat ggatagtgta cgacaaaaat
    34921 attatggaaa tgccaagtag atattataat ataatggaga atactggaca ggtgatttat
    34981 atacaaggac agggaatgaa taaagaattc tcggctatga tcacggacat attacctaat
    35041 ttccaattta ttggtaatgg caaaggcttt gctacataca aaggtaaaga tagtttgagg
    35101 cttgtagata atatttctaa ttcttttaaa aagaaaataa atcttaattc agaagagata
    35161 gtatattata tatatgcaat tttgcatcat aaatattatg tgaacaaata ttcaagtgat
    35221 ttgagtaaag gttttccgcg aataccgata ttaaaggatg tatatggatt tgttgaaatt
    35281 ggtagggagt tagtggaatt acatttaaat tatgaaaaac aactcaattg ggatggtgta
    35341 gaaataattt ataataatat gaatccgaat tataaagttg aaaaatgaag caccctaaaa
    35401 agggcgtgtt ggatacagta atatacaata ccgatattac agttactaac attccaaata
    35461 aagtatataa ttatattgtg aatggcaagc ctgctattga atggattata gatcagtaca
    35521 acgtaagcat cgacaagaaa tctggaatat tagatgatcc taatgaattt agtgaagatc
    35581 cgaattacat tttaaatctg cttttatcag ttattactgt aagtatgaaa actcttggat
    35641 taatagataa attaccagat tttgaagtat tagattaagg agaggatata ttgacaaata
    35701 ctatcaaaaa aataatttat tttgatgaac tatcagcaat ggatttatta cagatagaga
    35761 agaaaggtaa ccttactaaa acagttcagt tgatgcatga tgaaaaaggt agtgcaagtg
    35821 gtgaatctaa agtgaacatg gaatttggta agcaaagcgc tgtaaaacaa atttttgatt
    35881 ctttacttgg gatgtcagcg agtgcttcgg ctagtgcagg tataaatgga aatataactg
    35941 gagagcgagt agccaaaaca attttagaga atacattaat atatgatttt ttggatacag
    36001 tagagtttag aaagaaaaaa actattattg atatttcaga ggggtttaaa ctaaatattc
    36061 aacaagatac gatgacatat ttcgcgacta ttacccccat agcaagtatg tttgaaggat
    36121 ctaaacatct agacgaggag gagctatcat ttgatgtttc aaaaatgaat gaagcgatta
    36181 gaagtattaa aggctatttt gaattaatag gaacaagagg gaatgaaaac agagttttta
    36241 gatttaatat agaatctttt aaaaataact atagaataca agatttaaaa tttatgaatt
    36301 taacactcta ctcagtactt gttggtgaaa taagcaaaga aaatctaaat tacgataaag
    36361 agtttgatct aaatcaaggt gccaaaccta gtggaattgg aaagtttaag ggaattagat
    36421 cagaagcaac taaagaagat aaatatcaag tgtatgatgt tattcttgca ggagtgaaat
    36481 aaatggaaat tcatgtttat acgggaccgc tttgtggatt taatgaatat attaattctg
    36541 aagttaaaga atatcaccgc ttcatggaat taatcagaga atacaatatc atagtgcgag
    36601 caagtgatac atttggaact catgatgttg atggtatttt tgatactgag atagaaaatc
    36661 ttgtgatatt ttcaaacgac tttgcttctg taacaactca tgttattact aattttataa
    36721 atatcgttac tttaggtaga aatattaaga aaatctatat tcagaatcct ccaaaaagag
    36781 tatttcaatc actaaatagt tttcacgatt ctgaaataat agaaataaac tatgaatata
    36841 caacattaaa aaaagatgat gtaataaatt tttataaaga gcagatggct ggttctaaaa
    36901 taataggtca aacagacgct aaaaaacaaa tgagcataga tttatataaa catacttcct
    36961 tagaaaataa taaaccgacc gtaatgcttt actatggtcc atcaggtgtt ggaaaaacag
    37021 aattagccaa agaaatagca aaaaaatata aaggtaatat cactagaata caattttcta
    37081 tgatgcagaa tgatgaatct tttaattata tctttggaga tgaacatgcc aaagtatcat
    37141 tgtcaaaaga tttagtagat cgtgaaacta atgttgtttt aattgatgaa tttgataagg
    37201 tattaccaat ctattacaat gttttctatc aaatgtttga tgaaggtatt attgaagatg
    37261 ccaattataa agtagacgtt tcaaattgta ttttcattct aacatctaat tttaatagta
    37321 ttgaagaagc aatagttaat attggagaac cggtattttc tagaatagat tcctgtatta
    37381 aatttttata tttaacacag gaagaaaagc agaaagtaat agaaaataaa ttaaatgaaa
    37441 tactatcatc tctaaatga
//
LOCUS       DAMQYT010000010        36952 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMQYT010000010 DAMQYT010000000
VERSION     DAMQYT010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 36952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36952
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.010"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..91
                     /locus_tag="PGA33_000405"
     CDS             <1..91
                     /locus_tag="PGA33_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="subunit E of antiporter complex involved in
                     resistance to high concentrations of Na+, K+, Li+ and/or
                     alkali; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HDG6472244.1"
                     /translation="HSIDVSEKEKDSLLRSIKHYEDLILEVSR"
     gene            88..390
                     /gene="mnhF2"
                     /locus_tag="PGA33_000406"
     CDS             88..390
                     /gene="mnhF2"
                     /locus_tag="PGA33_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HDG6472245.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            365..802
                     /gene="mnhG2"
                     /locus_tag="PGA33_000407"
     CDS             365..802
                     /gene="mnhG2"
                     /locus_tag="PGA33_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HDG6472246.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAEKREEHRKKWYQND"
     gene            1142..3184
                     /locus_tag="PGA33_000408"
     CDS             1142..3184
                     /locus_tag="PGA33_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDG6472247.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
     gene            complement(4344..5273)
                     /locus_tag="PGA33_000409"
     CDS             complement(4344..5273)
                     /locus_tag="PGA33_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDG6472248.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(5270..6106)
                     /locus_tag="PGA33_000410"
     CDS             complement(5270..6106)
                     /locus_tag="PGA33_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDG6472249.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            complement(6100..6843)
                     /locus_tag="PGA33_000411"
     CDS             complement(6100..6843)
                     /locus_tag="PGA33_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDG6472250.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            6965..7609
                     /locus_tag="PGA33_000412"
     CDS             6965..7609
                     /locus_tag="PGA33_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDG6472251.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            complement(7689..8441)
                     /locus_tag="PGA33_000413"
     CDS             complement(7689..8441)
                     /locus_tag="PGA33_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HDG6472252.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYFMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            8677..9441
                     /locus_tag="PGA33_000414"
     CDS             8677..9441
                     /locus_tag="PGA33_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HDG6472253.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            complement(9502..10296)
                     /gene="tagH"
                     /locus_tag="PGA33_000415"
     CDS             complement(9502..10296)
                     /gene="tagH"
                     /locus_tag="PGA33_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HDG6472254.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            10643..11455
                     /gene="tagG"
                     /locus_tag="PGA33_000416"
     CDS             10643..11455
                     /gene="tagG"
                     /locus_tag="PGA33_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HDG6472255.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            11554..12657
                     /locus_tag="PGA33_000417"
     CDS             11554..12657
                     /locus_tag="PGA33_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDG6472256.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLVLYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEDSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDQVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            12654..13715
                     /locus_tag="PGA33_000418"
     CDS             12654..13715
                     /locus_tag="PGA33_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDG6472257.1"
                     /translation="MRFTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVIKPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNEE
                     DFNRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            13778..14176
                     /gene="tagD"
                     /locus_tag="PGA33_000419"
     CDS             13778..14176
                     /gene="tagD"
                     /locus_tag="PGA33_000419"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HDG6472258.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(14293..15588)
                     /gene="pbp4"
                     /locus_tag="PGA33_000420"
     CDS             complement(14293..15588)
                     /gene="pbp4"
                     /locus_tag="PGA33_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HDG6472259.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQATNSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGTCLVAGLALIVHMIINRLFRKRK"
     gene            16009..17736
                     /locus_tag="PGA33_000421"
     CDS             16009..17736
                     /locus_tag="PGA33_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6472260.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            18095..19324
                     /locus_tag="PGA33_000422"
     CDS             18095..19324
                     /locus_tag="PGA33_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDG6472261.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            19579..19671
                     /locus_tag="PGA33_000423"
     CDS             19579..19671
                     /locus_tag="PGA33_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472262.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            19849..20682
                     /locus_tag="PGA33_000424"
     CDS             19849..20682
                     /locus_tag="PGA33_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDG6472263.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            20964..21761
                     /locus_tag="PGA33_000425"
     CDS             20964..21761
                     /locus_tag="PGA33_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6472264.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            21797..22801
                     /locus_tag="PGA33_000426"
     CDS             21797..22801
                     /locus_tag="PGA33_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDG6472265.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            22798..23814
                     /locus_tag="PGA33_000427"
     CDS             22798..23814
                     /locus_tag="PGA33_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDG6472266.1"
                     /translation="MISSNNKRRQLIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDKLILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITTALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            24048..25016
                     /gene="dhaK"
                     /locus_tag="PGA33_000428"
     CDS             24048..25016
                     /gene="dhaK"
                     /locus_tag="PGA33_000428"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDG6472267.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            25058..25642
                     /gene="dhaL"
                     /locus_tag="PGA33_000429"
     CDS             25058..25642
                     /gene="dhaL"
                     /locus_tag="PGA33_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDG6472268.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            25635..25997
                     /locus_tag="PGA33_000430"
     CDS             25635..25997
                     /locus_tag="PGA33_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDG6472269.1"
                     /translation="MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            26113..26610
                     /locus_tag="PGA33_000431"
     CDS             26113..26610
                     /locus_tag="PGA33_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472270.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            27067..28134
                     /locus_tag="PGA33_000432"
     CDS             27067..28134
                     /locus_tag="PGA33_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472271.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            28285..29328
                     /locus_tag="PGA33_000433"
     CDS             28285..29328
                     /locus_tag="PGA33_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDG6472272.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
     gene            29657..30085
                     /locus_tag="PGA33_000434"
     CDS             29657..30085
                     /locus_tag="PGA33_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472273.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(30294..30800)
                     /locus_tag="PGA33_000435"
     CDS             complement(30294..30800)
                     /locus_tag="PGA33_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6472274.1"
                     /translation="MAHIIRRVSIKDVENFISMLANIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            30913..31836
                     /gene="graX"
                     /locus_tag="PGA33_000436"
                     /gene_synonym="apsX"
     CDS             30913..31836
                     /gene="graX"
                     /locus_tag="PGA33_000436"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HDG6472275.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            31852..32526
                     /gene="graR"
                     /locus_tag="PGA33_000437"
                     /gene_synonym="apsR"
     CDS             31852..32526
                     /gene="graR"
                     /locus_tag="PGA33_000437"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HDG6472276.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDQTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            32519..33559
                     /gene="graS"
                     /locus_tag="PGA33_000438"
                     /gene_synonym="apsS"
     CDS             32519..33559
                     /gene="graS"
                     /locus_tag="PGA33_000438"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HDG6472277.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNFLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLLLTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDD
                     YVYTDIKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEI
                     VERMSEVTNLSF"
     gene            33703..34464
                     /gene="vraF"
                     /locus_tag="PGA33_000439"
     CDS             33703..34464
                     /gene="vraF"
                     /locus_tag="PGA33_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HDG6472278.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            34454..36343
                     /gene="vraG"
                     /locus_tag="PGA33_000440"
     CDS             34454..36343
                     /gene="vraG"
                     /locus_tag="PGA33_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HDG6472279.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIY
                     ILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLI
                     GVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYL
                     SLNIVQYYDSIGTLMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSS
                     IMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQ
                     LASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGSIKDLVKHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLND
                     KDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKAKNKVDKSIETRSEAISSISSLTG
                     ILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIM
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRT
                     IRHSI"
BASE COUNT        12798 a         5137 c         6577 g        12440 t
ORIGIN      
        1 tcatagtatc gacgtgtcag aaaaagaaaa agatagtttg ttaagaagta ttaagcatta
       61 tgaagactta atattggagg tgtcgcgatg atacaaacaa taacacatat tatgattatt
      121 agttcactca ttatttttgg aattgcatta atcatctgtt tatttagatt aatcaaggga
      181 cctacaacag cagatcgtgt cgttacattt gatacaacaa gtgctgtcgt aatgtcaatt
      241 gtgggtgtgt taagtgtact tatgggcacc gtttctttct tagattcaat catgctcatt
      301 gccattatat cttttgtaag ttctgtttca atatcacgct ttattggtgg ggggcatgtg
      361 tttaatggaa ataacaaaag aaatctttag tcttattgct gctgtgatgt tgttgttagg
      421 tagttttatt gctcttatta gtgcaatagg tatcgtgaaa ttccaagatg ttttcttaag
      481 aagtcacgct gcgacaaaaa gttcaacttt atccgtgtta ttaactttaa tcggtgtatt
      541 aatttatttt attgtgaata caggattttt cagtgtgcgt ttattactgt cacttgtttt
      601 tattaattta acttcaccag tcggcatgca cttagtcgct cgcgctgctt atcgcaacgg
      661 cgcttatatg tatcgaaaaa atgatgctca cacacatgca tcaatattat taagttcaaa
      721 tgaacaaaac tctacagaag cattacaatt acgtgctgaa aaacgagaag agcatcgtaa
      781 gaaatggtat caaaacgatt gaagtgttgg ctaaattgtt aagcataaca atgcttttga
      841 aaatctgttt tcaaaattaa tcattaaaag agtactttga aattggttag tcgattttaa
      901 ggtgctcatt ttttgtatta taagttaatg ttgttatgtg aaagcaagtt gtttatttat
      961 acaaacaatt agtgataaaa taaaattaaa taattgcaaa tttaagtact gcaatcgtag
     1021 acatatatct atattaaagt gcttttgtgg tcgctaactc aatttatcct ctaaagtata
     1081 attacaacga gatgtgatac ttaattttca attaaacaat actttttaga gaggtgaaag
     1141 tttggaaata tttgaaacaa ttcttatatt tatagctgtt gtgatactaa gttcgtttgt
     1201 ccatactttc atacctaaag tacccctagc atttatacaa attttcttgg gcatgttact
     1261 atttattacc ccaatccctg ttcaatttaa ttttgattct gaattgttta tggtaacaat
     1321 gattgcgcct ttgttatttg tagaaggtgt taatgtttct agagtccatt taaggaaata
     1381 tattaagcca gtgatgatga tggcattagg attagtcatt actactgtga taggtgtagg
     1441 tttatttatt cattggattt ggccagattt acctattgga gcagcatttg caattgctgc
     1501 cattctttgt cctactgatg cagtagcagt gcaagcaatc actaaaggaa aggtcttgcc
     1561 aaaaggagca atgacaattc ttgaaggtga gtcattattg aatgatgctg ctggtattat
     1621 ttcatttaaa atagctgttg gagtattagt tacaggtgct ttttcacttg ttgatgctgt
     1681 tcagttgttt ttaattgcat caattggtgg cgcagtggtt ggtttactta taggtatggc
     1741 attagtaagg ttccgattaa cattgatgcg tcgaggatat gaaaacatta atatgtttac
     1801 aattattcaa ttgttaacac catttgttac gtatttaatt gctgaattgt ttcacgcatc
     1861 aggaatcatt gcagcagtag ttgcaggact tgtacatggt ttcgaacgtg acagaattat
     1921 gcaagtacgt acacaactgc aaatgagtta caatcataca tggaatatac taggttatgt
     1981 tttaaatggc tttgtttttt caatattagg atttttagta cctgaagtta ttattaaaat
     2041 tatcaaaaca gaaccgcaca atttaatctt tttaataggc atcactattg ttgttgcttt
     2101 agctgtctat ctatttagat ttgtttgggt ttatgtctta tatccttatt tttatttagc
     2161 catcagtcca ttccaaaaaa tgatgactaa aaatgatgat gataatccaa cgactgagaa
     2221 accaccaaag cgaagtttat acgctttaat tatgacgtta tgtggtgtgc atggaacaat
     2281 ttctttagca atcgcattaa cgttaccgta ttttttagca gggcatcatg cttttacgta
     2341 tagaaacgac ttattattta ttgcatctgg tatggttatt attagtttgg tagttgcgca
     2401 agtattattg ccattattaa cgaaacctgc acctaaaaca gtaattggca atatgtcgtt
     2461 taaagttgct agaatttata tattagaaca agttattgat tatctaaatc aaaaatctac
     2521 tttcgaaaca agttttaaat atggtaacgt gattaaagaa tatcatgata aattagcatt
     2581 tttaaaaact gtagagaaag atgatgaaaa ctctaaagaa ttagaacgtc tacaaaaaat
     2641 tgcttttaat gtagaaacaa aaacattaga gtctttagta gatgaaggac aaataacgaa
     2701 tagtgtactt gaaaactata tgcgttatgc tgaaagaaca caggtatata gacaagcatc
     2761 attaataaga agaatgattg tattattacg aggtgcttta ttaaaacgaa gagtacaaac
     2821 gagagtgaac tccgcatctt cacttagtgt tacggataac ttaatggaat taaataaaat
     2881 taataaatta gtccattata atgtggttag tcgtttgtct aaggaaacaa caaaagataa
     2941 tacacttgaa gttggaatgg tttgtgacgg ttatttaatg cgaattgaaa acttaacacc
     3001 atcaaatttc ttcaactcag caagtgaaga tacgattact aaaattaaat taaatgcatt
     3061 gagagaacaa cgtcgcattt tacgtgagtt gattgataca gatgaagtat cagaaggtac
     3121 agcgttaaaa ctaagagaag ccatcaatta tgatgaaatg gttattgtag atagtatgac
     3181 gtagttccta attatgctaa aagggattga tgaaaaactg aagggctttt catcaatccc
     3241 ttttatttta ggggaattga atagatagtt ttaaactata cgaattatta atatttgaga
     3301 tttaattgaa ataagtttta aaaattggag gagatagatt aagcgaagtc atttaaaggt
     3361 gaagttaagt gtattcacaa aaaatagcca cactcatatg acatcggatg agtgtggctt
     3421 aaggatctat ggggggagga aaccatagat gtttactttg ataggccaga ttaaatatca
     3481 aagtatgcga ttatttatag cttgatgcaa aagtggtatg cctattaaaa gttactgcac
     3541 atagctttta atattccgtt caaaggaaag gggcatacaa ttgaacaatc tgtaatagta
     3601 cttttaacca gctatgctaa aagtctagta gggagaacag ttgtccaatc acataagaac
     3661 ctctaacttc gttagtacga ttaagaaaag ctttttagtt agtatgtaat acaatttatt
     3721 gacgcgcgtg aatctctttt ataagagtgt gtagggaatg gcgttgtata aattgtatta
     3781 gaagaacttc taacgcatct ctgtggttaa aagagatgaa gggaacgaca gtttaattaa
     3841 aactgcataa gaacttctag cttttctctc tcgttcaaag agaagcagct gttcgcagtt
     3901 taatcaaaac cacataaagc ttttaacttt actctttgat ttaaagagtg ataaatgttt
     3961 acagtttaat taaaactgca taagaacttc tagcttttct ctttcgttca aagagaagca
     4021 gctgttcgca gtttaatcaa aaccacataa agcttttaac tttactcttt gatttaaaga
     4081 gtgacaaatg tttacagttt aattaaaact gcataagaac ttctagcttt tctctttcgt
     4141 tcaaagagaa gttctaatac caccatatcg tgcgatcggg aacggtatat atattaatag
     4201 gagggtaata tatatttaac gcacgatatg ggactattag ccttcgactt tgttatgttg
     4261 atgtgtggcc taaaatattg gagataccaa tattttaggt tgcatcaaca tcaattcatc
     4321 ttacttcatt aaaacacagc gtgttatttc atgcttccgt gtacagtttc aatatttgat
     4381 ttcatcattt tgtagtaaga gtcaccttta gtgccttctt taccgattga atctgtgtac
     4441 acttcaccaa agatatcttt cttcgtttct tcagataaac tttccattgc tttcttatca
     4501 acacttgttt ctactaataa gtgttttaat ttgtgctttt taacaaactc aatagcttgt
     4561 ctcatttgtt caggtgtacc ttgtttttca gtgttaattt cccaaatata acctggtgta
     4621 ataccgtatt gttttgagaa gtacttgaag gcaccttcac ttgtaatcat ggcacgttgt
     4681 tcttttggaa tgtcattaaa tttgtcttta ctgtcattat ttaatttttc caattgagca
     4741 atgtatttgt taccttgctt ttcataatct gctttatgtt ttttgtcgtt atcgataaat
     4801 gtttgttgaa ttgtttttac gtatttaata ccgttatcta aacttaacca tgcgtgtgga
     4861 tcttgtttat ctttgttgcc ttcttcaccg tttaaataga taggtttaac atcttttgat
     4921 actgcgataa cttttttatc ttttaatgat ttaccagcct gttctaaggc tttttcaaac
     4981 caaccgttac cagtctctaa atttaatccg ttgtataaaa taacgtcagc gtcagttaac
     5041 tttttaatat ctttaggttt aacttcatat tcatgaggat cttgaccaac aggtacaata
     5101 ctatgaatat cgacgttgtc tccaccaaca tttttagcca tatcatataa aattgaattc
     5161 gtcgttacta cttttaattt gccatttgac ttatcactgc tttgtttacc accagtacca
     5221 catgcagcaa ctagaagtaa taaggctaat aataaaggta ctaatttttt catgttaaac
     5281 ttcctcgttt ctttctattc gtaaattttg tgaaaaataa tgtgatgata taaattacaa
     5341 acgtacaaag tacgattgtc gcaccactag gaatgttgta aatatagctg taataaagtc
     5401 cgacaattga acttatgaca cttattaaac ttgctataat catcattgag tatagttttt
     5461 tactaattaa aaatgctgta gatgcaggtg taattaataa tgcaactaca agaataatac
     5521 ctaccgtttg aatacttgct actgttacta atgagagtaa caacatcaca aagtaatgta
     5581 ataacgtcgt atttagacca ctcattctac taaacgttgg atcgaatgta gaaatcatta
     5641 atggacgata gaaaataatg attagaataa ggacgattga accaatcaca atagttgtta
     5701 aaaatgcact atttgtgatt gccagtaaat taccaaacag aatatggtac aaatctgtcg
     5761 tagtgtttat taagctaata ataataatcc ccgaagctaa gaaagcggta aaactaattc
     5821 caatagcggc gtcaggtttc gttttactac tagatgtgat ataaccgata aaaatacttg
     5881 cgatcatacc agttataagt gcgcctacaa acattggaat accaaataag aatgataggg
     5941 caacaccagg taatactgcg tgactcattg catctcccat taatgaaaga ccacgtaata
     6001 caattaaact accaactgta ccacaaacta tccctacaat aattgaagtt atcaatgctc
     6061 gattcaagaa ttgatatgta aataaatgtt cgacaaactc taacatgtta tattgctcct
     6121 ttgactaggg tcactacagt cagtgctact cataaatgtt tcgtttaagc gagtgacact
     6181 catagcctct tcactatcac caaagtatcg taatgtttga tttaatagaa taatgcgatc
     6241 aaagtattgc tttgcttttg atagatcatg gtggatgata agaataagtt ttccttgttg
     6301 ttttaagttc tcgatttttg tcatgattaa tttttcgcta ctaaaatcaa ttccgacaaa
     6361 cggctcatct agaaaataaa cttcactttc ggacatcaat gctcttgcta ctagcacacg
     6421 ttgtaattgt ccaccactta attctgaaat ttgtcgatga cgtaaagatt ctaattctaa
     6481 atcgcttaat aactgtttga gtttatccct tgctgattta ttaggtcgtc taaaccatcc
     6541 aatttctttg tagcaacctg ataaaatcac ttgttccaca cttataggaa aatctaaatc
     6601 aatatgtgct ttttgtggaa tatatgtaat atgttgcagt tgttgttgta taggtttgtt
     6661 atataacaat ttagtaccgg tagcattaaa ttcaccaatt aaagacttga taagggaaga
     6721 tttaccagca ccattcgggc ccatgatacc aattatttcg ccgcgtactg gtatcgataa
     6781 ggaaatgttt ttaagtacat gcttattacc taaaaacaga tttaaatctt ttgtttctaa
     6841 caaacgttta tacctcctaa ttaaaagttt aggctaacct aattaattgt ataataaact
     6901 gagaatattt atcatgtcaa gtaaattcgt gatataatat agacaatgta tgtgaggtga
     6961 aagtatgtta actgaagaaa aagaggacta tttaaaggca atccttacga ataatggcga
     7021 taaaaacttt gtgacaaata aaatcttatc tcaattttta aatattaagc ctccatctgt
     7081 aagtgaaatg gtaggacgtc ttgaaaaagc aggctatgtt gaaacaaaac catacaaagg
     7141 tgttagatta acagaggatg gtttaacgca tacgcttgat atcattaaga gacatcgact
     7201 attagaatta tttttaatag aaatattgaa atataattgg gaagaagtac atcaagaagc
     7261 agaaatttta gaacatcgaa tttcagattt atttgttgaa aggctggata gcctgttaaa
     7321 tttcccagaa acttgcccgc acggcggtgt gattcctaga aataatgaat ataaagagaa
     7381 atatataaca acgattttga attatgaacc tggtgatatc gttacaatca aacgtgtgag
     7441 agataagacc gatttgctaa tatatttgtc tagtaaagat atttctattg gtaatgaagt
     7501 ggaaattgta tcgaaagatg aaatgaataa agtaattatc attaaacgta atgataatgt
     7561 aattattgtc agttacgaaa atgcaatgaa catgtttgct gaaaaataaa ataaagaagc
     7621 cataaagata tccatgattg aactgataaa gacatatgga taattgcttt aggcttcttt
     7681 tttattagtt aatttatcaa gtgagtatat ttgagtaaaa tattcactgc ataaagattg
     7741 aagataatcc agattgtact ataaatgaag ataggtacat gactgagttc tttaagtgca
     7801 ctaccatccc actgtggact cggacgctgg aaagtcaatt tagcaatcgt ccaactagat
     7861 tgtagaactt cgcctaataa tacacctaaa atatattgat aactcattgt gacaagtagt
     7921 tgaatttcta ctatattttc atcttttaat ataaaataca acatgataga aattaaagtt
     7981 ataacaacaa tgggtgagcc ttttctagat gttaaaatta aaaaataaat aaatatcaat
     8041 aaataggtaa atataaagaa actaggtatc tgataatggc tcgacgctaa acctatcaat
     8101 aacataatag gtggcataaa ataaccacca atcgttgtaa gccattggcc tgctagatgt
     8161 ctagattgtg taattgcgaa tccttgttgt aatgtctgtt gtcgctctcg tggacttgtt
     8221 acaatgacta aatcttttgc acggccacca gcgagtttat taaacagtac atgaccaaat
     8281 tcatgtgtta aaacagggat atagtttaaa atgacatcta aatagttcaa aacaggctta
     8341 tgtctatatt gatgaatagc aatataacaa gctgcaacaa taacgataat gtatatatta
     8401 agttgaattg tcgtattaaa aaagtttgat aaataattca ttgttaacct catataagat
     8461 attaatttaa agtttgctta tcacttatta taaatgatat tggcatcaat agcgttagac
     8521 tttagactta ccttagttaa actaatttta atttttgaaa aggtgaatat gtgttaaaat
     8581 aaagcaaaat catttcgata taaataggat gaatataaat actgttaata ttgattacac
     8641 taacataata atgaaataag ataggagatt cctgttatga ctgttgaaga aagatccaat
     8701 acagccaaag ttgacatttt aggggtcgat tttgataata caacaatgtt gcaaatggtt
     8761 gaaaatatta aaaccttttt tgcaaatcaa tcaacgaata atctttttat agtaacagcc
     8821 aaccctgaaa tagtgaatta cgcgacgaca catcaagcgt atttagagtt aataaatcaa
     8881 gcgagctata ttgttgctga tgggacagga gtagtcaaag cttcgcatcg tttaaagcaa
     8941 cctctagcgc atcgtatacc tggtattgag ttgatggatg aatgtttgaa aattgctcat
     9001 gtaaatcatc aaaaagtatt tttgctaggg gcaactaatg aagttgtaga agcggcacaa
     9061 tatgcattgc aacaaagata tccaaacata tcgtttgcac atcatcacgg ttatattgat
     9121 ttagaagatg agacagtagt gaaacgaatt aaactgttta aacctgatta catatttgta
     9181 ggtatgggat tccctaaaca agaagaatgg attatgacac atgaaaacca atttgaatct
     9241 acagtgatga tgggcgtagg tggttctctt gaagtatttg ctggggctaa aaagagagcg
     9301 ccttatatct ttagaaaatt aaacattgaa tggatatata gagcattaat agattggaaa
     9361 cgtattggta gattaaagag tattccaata tttatgtata aaatagccaa agcaaaaaga
     9421 aaaataaaaa aggcgaaata atcatgatga caaaaataaa accgaggaaa tccttaaatg
     9481 gagattctcg gttttttcgg tttatttaat aacgaagcgg gactcatcga gtttgtttct
     9541 aaattctttt tgttcggctt tggatttctt tttaaaatcg ttaaggaaag cttcatattt
     9601 aggtaataca tcatcaagtt caccgtaatc ttttaacttt ccgccttcaa tccaagcaat
     9661 cttagtacaa aattgtctca cttgtcctaa gttatgacta acgaaaaaga tggttttgtt
     9721 ttgctcttta aactcgtaaa ttttatctaa acatttttgt gcaaaagttt ggtcacctac
     9781 agataaagct tcgtcaatga ctaagatatc tggattaact gtgatattaa ttgaaaaacc
     9841 aagttttgca cgcataccac ttgaatactt tttaactggt tgataaataa actcaccaag
     9901 ttcactaaat tcaataatct taggtgtcat cgctttaatt tcttttcgct taaagcccat
     9961 acataacatt ttaaattcga tattttcaat ccctgtaagt tgtccactca agccagcact
    10021 aattgcgata acgctgactt caccattacg atccactttg ccaacagtag gcgacaaaga
    10081 accgccaatg atattgctca acgttgattt gccggaacca ttgatgccaa caagccctat
    10141 gacgtcacct tcatatgctt ttaaactaat gtcatctaaa gcgaaaaatg ttttgttttt
    10201 atgtttggga atgagcgcat ctttcatacg ttctttattt gtacgataaa tacgatattc
    10261 ttttgttaca tttttaatgt ttaccgaaac gttcatttgt agaccttcct tattcacatt
    10321 tatctagatt ataatatact actcaacagt tgttaaattt taaaacctgt tgtaaagtgt
    10381 atagaagatt ttgttattat cagagtgggt gttttgacac aaaatgttaa tcatcaatga
    10441 taacaatgat atttaaaaac taaacttatt tcaacttaca tgattgtata ctataatgta
    10501 tttgtaataa actaatattt taaagaacta gacaataatt ttgatagcat ccatgtatag
    10561 tgatagtatt tacaacaatt attataatac tatttagtta agtagagaaa tagttaaaca
    10621 tttgaaagtg tggtttaatg gaatgtcagc aataggaaca gtttttaaag aacatgtaaa
    10681 gaacttttat ttaattcaaa gactggctca gtttcaagtt aaaattatca atcatagtaa
    10741 ctatttaggt gtggcttggg aattaattaa ccctgttatg caaattatgg tttactggat
    10801 ggtttttgga ttaggaataa gaagtaatgc accaattcat ggtgtacctt ttgtttattg
    10861 gttattggtt ggtatcagta tgtggttctt catcaaccaa ggtattttag aaggtactaa
    10921 agcaattaca caaaagttta atcaagtatc gaaaatgaac ttcccgttat cgataatacc
    10981 gacatatatt gtgacaagta gattttatgg acatttaggc ttacttttac ttgtgataat
    11041 tgcatgtatg tttactggta tttatccatc aatacatatc attcaattat tgatatatgt
    11101 accgttttgt tttttcttaa ctgcctcggt gacgttatta acatcaacac tcggtgtgtt
    11161 agttagagat acacaaatgt taatgcaagc aatattaaga atattatttt acttttcacc
    11221 aattttgtgg ctaccaaaga accatggtat cagtggttta attcatgaaa tgatgaaata
    11281 taatccagtt tactttattg ctgaatcata ccgtgcagca attttatatc acgaatggta
    11341 tttcatggat cattggaaat taatgttata caatttcggt attgttgcca ttttctttgc
    11401 aattggtgcg tacttacaca tgaaatatag agatcaattt gcagacttct tgtaatatat
    11461 ttatatgacg aaaccccgct aaccattaat aaatggaagt ggggttcatt tttgtttata
    11521 atttaagtaa ataacatatt aagttggtgt attatgaacg ttttaataaa gaaattttat
    11581 catttggtag ttcgaatact ttctaaaatg attacgcctc aagtgattga taaaccgcat
    11641 atcgtattta tgatgacttt tccagaagat attaagccta tcatcaaagc attaaataat
    11701 tcgtcgtatc agaaaactgt tttaacaaca ccaaaacaag cgccttattt atctgaactt
    11761 agcgacgatg ttgatgtgat agaaatgact aatcgaacat tggtaaaaca aattaaggct
    11821 ttgaaaagcg cgcagatgat tattatcgat aattattacc tattactagg tggatataat
    11881 aagacttcta atcaacacat tgttcaaacg tggcatgcaa gtggtgcatt aaaaaacttt
    11941 ggcttaacag atcatcaagt cgatgtgtct gacaaggcaa tggttcagca gtaccgtaaa
    12001 gtttatcaag cgacggattt ttacttagtg ggttgtgaac aaatgtcaca atgttttaaa
    12061 cagtctttag gtgcaacaga agagcaaatg ctgtattttg ggcttccgag aattaataaa
    12121 tattacacag ctgatagagc aacggttaag gcagagttaa aggataaata tggaattaca
    12181 aataagttgg tattatatgt accaacatat agagaagata aagcagataa tagggctatt
    12241 gataaagctt attttgaaaa atgtttacca ggatatacac tgattaataa attacatcca
    12301 tcaattgaag attcagacat tgatgacgta tcttcaatcg acacgtctac attaatgcta
    12361 atgtcagata taattattag cgactatagt tcgctgccaa tagaagctag cttgttagat
    12421 attccaacta tattttatgt gtatgatgaa ggaacatatg atcaggtgag aggcctgaat
    12481 caattttaca aagcaatacc ggatagctac aaagtgtata ctgaagaaga tttaataatg
    12541 acgatacaag aaaaagaaca tctattaagt ccgttattta aagattggca taagtataat
    12601 actgataaaa gtttacatca gctcacagaa tatatagata agatggtgac aaaatgaggt
    12661 ttacgataat catacctaca tgtaataatg aggcaacaat tcgacaattg ttaatatcta
    12721 ttgagagtaa agaacactat agaatccttt gtattgatgg tggttctact gatcaaacaa
    12781 ttcctatgat tgaacggtta caaagagaac tcaagcatat ttcattaata caattacaaa
    12841 atgcttcgat agctacgtgt attaataaag gtttgatgga tatcaaaatg acagatccac
    12901 atgatagtga cgcatttatg gtcataaaac caacatcaat cgtattgcca ggtaaattag
    12961 ataggttaac tgctgctttc aaaaataatg ataatattga tatggtaata gggcagcgag
    13021 cttacaatta ccatggtgaa tggaaattga aaagtgctga tgagtttatt aaagacaatc
    13081 gaatcgttac attaacggaa caaccagatt tgttatcaat gatgtctttt gacggaaagt
    13141 tattcagtgc taaatttgct gaattacagt gtgacgaaac tttagctaac acatacaatc
    13201 acgcaatact tgtcaaggcg atgcaaaaag ctacggatat acatttagtt tcacagatga
    13261 ttgtcggaga taacgatata gatacacatg ctacaagtaa cgaagaagat tttaatagat
    13321 atatcacaga aattatgaaa ataagacaac gagtcatgga aatgttacta ttacctgaac
    13381 aaaggctatt atatagtgat atggttgatc gtattttatt caataattca ttaaaatatt
    13441 atatgaacga acacccagca gtaacgcaca cgacaattca actcgtaaaa gactatatta
    13501 tgtctatgca gcattctgat tatgtatcgc aaaacatgtt tgacattata aatacagttg
    13561 aatttattgg tgagaattgg gatagagaaa tatacgaatt gtggcgacaa acattaattc
    13621 aagtgggcat taataggccg acttataaaa aattcttgat acaacttaaa gggagaaagt
    13681 ttgcacatcg aacaaaatca atgttaaaac gataacgtgt acattgatga ccataaactg
    13741 caatcctatg atgtgacaat atgaggagga taacttaatg aaacgtgtaa taacatatgg
    13801 cacatatgac ttacttcact atggtcatat cgaattgctt cgtcgtgcaa gagagatggg
    13861 cgattattta atagtagcat tatcaacaga tgaatttaat caaattaaac ataaaaaatc
    13921 ttattatgat tatgaacaac gaaaaatgat gcttgaatca atacgctatg tcgatttagt
    13981 cattccagaa aagggctggg gacaaaaaga agacgatgtc gaaaaatttg atgtagatgt
    14041 ttttgttatg ggacatgact gggaaggtga attcgacttc ttaaaggata aatgtgaagt
    14101 catttattta aaacgtacag aaggcatttc gacgactaaa atcaaacaag aattatatgg
    14161 taaagatgct aaataaatta tatagaacta tcgatactaa acgataaatt aacttaggtt
    14221 attataaaat aaatataaaa cggacaagtt tcgcagcttt ataatgtgca acttgtccgt
    14281 ttttagtatg ttttattttc tttttctaaa taaacgattg attatcatat gaacaataag
    14341 tgctaatcca gcgacaaggc atgtaccacc aatgatagtg aataatggat gttcttccca
    14401 catactttta gcaacagtat ttgccttttg aataattggc tgatgaactt ctacagttgg
    14461 aggtccataa tctttattaa taaattctct tggatagtcc gcgtgtactt taccatcttc
    14521 gactacaagt ttataatctt ttttactaaa atcacttggt aaaacatcgt aaagatcatt
    14581 ttcaacataa tatttcttac catttatcct ttgctcacct ttagacaata tttttacata
    14641 tttatactga tcaaatgagc gttccattaa tgcattcccc atcatattac gttgcttctc
    14701 gccaccaagg tttttatagt ctcctgcacc catgataact tgattaattc taaatttacc
    14761 tcgtttggta gtaatcgtat ggttgtaatt tgctgtatca cttgatccag tttttaaacc
    14821 atctgtaccc ggcaaactca tttttgcacc ttccaatgaa aagttgaatg tgtaatacgt
    14881 aactgcatgc gttgttggtg ctaactgctt tgtaaagtct aatattttag gtgtctcttt
    14941 aatcacgtgt aaatctaaaa tggcatagtc tctagcagtc gttacagtac gttcttggtc
    15001 tttatacttt gttggtgcaa atgtacgtaa tcttgaattt tcagcacccg ttggattgac
    15061 gaaatgtgta tttttcattc cgatagcttt agctttgtta ttcattaaat caacgaaatc
    15121 gctggtgttt tttgaaacct tcttagctaa aattaatgcc gcggcattac tagaattaga
    15181 tactgtaatt tgtaataggt ctgcgattgt ccatacttgt ccaggatata gtttcgtatt
    15241 actcaactca ggtagtgtag acataatata ttctttgttc gtcattgtga ctgtgtcatc
    15301 aagtgaaagc tgccccttat ttacagcttc caatgttaag tacattgtca ttaatttagt
    15361 catagacgct ggattccact tagtatcgat attgtattga tacagtaatt gtccagtttg
    15421 acttacatta acagcactcg tcggttcgta tgcagccgac aaacctgcat aaccatattg
    15481 atttgctgct tgtacagggg ttacgtcact gttagtagct tgtgcatatg gtgtcataat
    15541 acttaatgtt aaacataaaa tgatgataat agatattaaa tttttcataa agcgttaatc
    15601 ttcccttttc caattcttaa atattcccta aaagcaatgg ttattcctac ttacggaaat
    15661 cattgctaat tcacttcacc ttaattaaat tgttgaaaat aaagttttct gcagttaatt
    15721 tgaaaaataa tgcaaatata ttacgtgtgt agctaaaggt gttataatgt ttgtacgaag
    15781 agcaaactta ctcaaaagcg attaattttc atgttttaat ataaagactt tgagaagtta
    15841 ttacaaaaaa tgcaatagaa atattctatc atataaatgt tatgagcggt attttggggc
    15901 aacactttat ttgattttta aagttttgtt gggagaaagt atatgataga aatgcatgta
    15961 tctatctaaa tgaattaact ataaatttca aacagaagag gtaaaactat gaaacgagaa
    16021 aatccattgt ttttcttatt taaaaaacta tcatggccag tgggtcttat cgttgcagct
    16081 atcactattt catcactagg gagcttaagt ggactattag tgccactgtt tactggacga
    16141 attgtagata aattttccgt gagccatatc aattggaatc taatcgcatt atttggtggt
    16201 atctttgtca tcaatgcttt attaagcgga ttaggtttat atttattaag taaaattggt
    16261 gaaaagatta tttatgcgat acgctcagtt ttatgggagc atatcataca attaaaaatg
    16321 ccattctttg acaaaaatga aagtggtcaa ttaatgagtc gattaactga cgatacgaaa
    16381 gtgataaatg aatttatttc acaaaagcta cctaacttat taccatcaat cgttacatta
    16441 gttgggtcac taatcatgtt atttatttta gattggaaaa tgacattatt aacatttata
    16501 acgataccga tattcgtttt aattatgatt cctctaggtc gtattatgca aaagatatcg
    16561 acaagtacac aatctgaaat tgcaaacttc agtggtttgt tagggcgtgt cctaactgaa
    16621 atgcgtcttg ttaaaatatc aaatacagag cgtcttgaat tagataatgc acataaaaat
    16681 ttgaatgaaa tatataaatt aggtttaaaa caggctaaaa ttgcggcagt tgtacaacca
    16741 atttcaggta tagttatgtt gctaacaatt gcaattattt taggttttgg tgcattagaa
    16801 attgcgactg gtgcaatcac tgcaggtaca ttaattgcaa tgatatttta tgttattcag
    16861 ttatctatgc ctttaatcaa tctttccacg ttagttacag attataaaaa ggcagtcggt
    16921 gcaagtagta gaatatacga aatcatgcaa gaacctattg aaccgacaga agctcttgaa
    16981 gattctgaaa atgtattaat tgatgacggt gtattgtcat ttgaacatgt agactttaaa
    17041 tatgatgtga agaaaatatt agatgatgtg tcgttccaaa tcccacaagg tcaagtgagt
    17101 gcttttgtag gcccttctgg gtctggtaaa agtacgatat ttaatctgat agaacgtatg
    17161 tatgaaattg agtcaggtga tattaaatat ggccttgaaa gtgtctatga tatcccgtta
    17221 tctaagtggc gacgcaaaat tggatatgtt atgcaatcaa attcgatgat gagtggtaca
    17281 attagagaca atattttata cggaattaat cgtcatgttt cagatgaaga acttattaat
    17341 tatgctaaat tagcgaactg tcatgatttt atcatgcaat ttgatgaagg atatgacacg
    17401 cttgtaggtg aacgaggatt gaaactgtct ggcggacaac gtcaacgtat tgatattgct
    17461 agaagttttg ttaaaaatcc tgatattttg ttacttgatg aagcaacagc taatctcgat
    17521 agtgaaagtg aattgaaaat tcaagaagct ttagaaacat tgatggaagg tagaacaacg
    17581 attgtcattg cgcatcgttt gtctacaatt aaaaaagccg gtcaaattat attcttagac
    17641 aaaggacagg taacaggtaa aggtacgcat tcagaactga tggcatcaca tgcgaagtat
    17701 aaaaactttg tagtgtctca aaaattaaca gattaatttt atatatataa gtaagcttgg
    17761 agcaaataca catataccat cgaggaaatt aaagtgtggc acattgatgg atatagatgt
    17821 taataaattg cttcaagctt ttgtctattt taaatcattt gagaagttac gacataataa
    17881 ttcttaaatt aatgaaatcg atattttaag aaaaaaatgc tcatggtata atacaagtta
    17941 taagcaaaca tacatatatt aaatactgta gccacgagtc ataattcttc gtattttaca
    18001 tagcaattta actgatttta gagtccacgg tacagaagtt tgatatttca atgtttctaa
    18061 atttttaaaa aattaaatca taggtgggtg ccaaatgttt ttattaatca acattattgg
    18121 tctaattgta tttcttggta ttgcggtatt attttcaaga gatcgcaaaa atatccaatg
    18181 gcaatcaatt gggatcttag ttgttttaaa cctgttttta gcatggttct ttatttattt
    18241 tgattggggt caaaaagcag taagaggagc agccaatggt atcgcttggg tagttcagtc
    18301 agcgcatgct ggtacaggtt ttgcatttgc aagtttgaca aatgttaaaa tgatggatat
    18361 ggctgttgca gccttattcc caatattatt aatagtgcca ttatttgata tcttaatgta
    18421 ctttaatatt ttaccgaaaa ttattggagg tattggttgg ttactagcta aagtaacaag
    18481 acaacctaaa ttcgagtcat tctttgggat agaaatgatg ttcttaggaa atactgaagc
    18541 attagccgta tcaagtgagc aactaaaacg tatgaatgaa atgcgtgtat taacaatcgc
    18601 aatgatgtca atgagctctg tatcgggagc tattgtaggt gcgtatgtac aaatggtacc
    18661 aggagaactg gtactaacgg caattccact aaatatcgtt aacgcgatta ttgtgtcatg
    18721 cttgttgaat ccagtaagtg ttgaagagaa agaagatatt atttacagtc ttaaaaacaa
    18781 tgaagttgaa cgtcaaccat tcttctcatt ccttggagat tctgtattag cagcaggtaa
    18841 attagtatta atcatcatcg catttgttat tagttttgta gcgttagctg atctatttga
    18901 tcgttttatc aatttgatta caggattgat agcaggatgg ataggcataa aaggtagttt
    18961 cggtttaaac caaattttag gtgtgtttat gtatccattt gcgctattac tcggtttacc
    19021 ttatgatgaa gcgtggttgg tagcacaaca aatggctaag aaaattgtta caaatgaatt
    19081 tgttgttatg ggtgaaattt ctaaagatat tgcatcttat acaccacacc atcgtgcggt
    19141 tattacaaca ttcttaattt catttgcaaa cttctcaacg attggtatga ttatcggtac
    19201 attgaaaggc attgttgata aaaagacatc agactttgta tctaaatatg tacctatgat
    19261 gctattatca ggtatcctag tttcattatt aacagcagct ttcgttggtt tatttgcatg
    19321 gtaatatgtc gaagagtgac tatgataata cattttaact aataaatatg tccaggcatg
    19381 tcgtctattg atataggtga gatgcttgga cttttttatt attgatataa aggtatttaa
    19441 atatttttaa agttaccgaa attgaagcat tataaaaacc aagtgcacat ggtaatacac
    19501 ttggctttta tgggaaatga atattattgt acatatgaca gtaaggacta ggtacagtca
    19561 tagtacttcg agcaaaattt gttttgttat tataaacaac acaaaggaga taacttctct
    19621 attgaagaag ttaaaaacat tatagcagac aatgaaatga aagtaaatta aaaattcaga
    19681 atatttttaa ttattatatt gtgagtgata tttattaggg aaagctattc ttcatataaa
    19741 ttagttaaat agtaaatctt tgttagaact ctttccatag agttgtgaca gcatttttgt
    19801 tgtgctacta tttttatagt ctaataaata tataaagggg atggtttcgt gaataaaacg
    19861 gttaaagatt taatactagt tgtcttaggt tcatttatct ttgctgcagg tgtaaatgca
    19921 tttattattt ctggtaactt aggtgaaggc ggggttacag gtttagcaat tattttatat
    19981 tatgcgtttc atatttcacc agccatcact aacttcttgg tcaacgcagt attgattgcc
    20041 ataggttata aatttttgag taagagaagt atgtacttaa ctattcttgt aacaattctt
    20101 atttcaatat ttttgagttt aacagaatca tggcaagtag aaactggaaa cagcattgtg
    20161 aatgccattt ttggtggtgt aagcgttgga ctaggaatcg gagtaattat ccttgcaggc
    20221 ggtacaacag caggtacaac aattttggcg agaattgcaa cgaaatacct cgatgtaagc
    20281 acgccatatg ctttgctttt cttcgatatg atcgttgttg caatttcact tacagttatt
    20341 ccacttgata aagtattagt aacagtaata tcactttata taggaacaaa agtgatggaa
    20401 tatgtcatag aaggtttaaa cactaaaaaa gctatgacga ttatttcaac taatcccgac
    20461 aaacttgcca aagcaataga cgagcaaatt ggaagaggtt taaccatttt aaacggacat
    20521 ggctattata cgcgtgaaga aaaagatgtc ttatacgttg ttatttctaa aacacaagtt
    20581 tcaaaagcaa agcgattaat taaacaaatc gataaagatg cattcctcgt aattcatgat
    20641 gtaagagatg tctatggtaa tggctttctt gcagatgaat aaataaatgg tatgagcaca
    20701 catacttaaa tagaagtcca cggacaagtt tttgaactat gaagacttat ctgtgggcgt
    20761 tttttatttt ataaaagtaa tatacaagac atgacaaatc gagctatcca atttaaaaag
    20821 taatgttagt caataagatt gaaaaatgtt ataatgatgt tcatgataat cattatcaat
    20881 tgggatgtct ttgaaaattg ataatttaaa aatagaaatt attttttata aacagaaaga
    20941 attttattga aagtagggaa attatgaatc gtttgcatgg acaacaagtt aaaattggtt
    21001 acggggataa cacgattata aataaattag atgttgaaat accagatggc aaagtgacgt
    21061 caatcattgg tcctaacggc tgcgggaaat ctactttgct aaaggcattg tcacgtttat
    21121 tggcagttaa agaaggcgaa gtatttttag atggtgaaaa tattcataca caatctacga
    21181 aagagattgc aaaaaaaata gccattttac ctcaatcacc tgaagtagca gatggcttaa
    21241 ctgttgggga attagtttca tatggtcgtt ttccacatca aaaaggattt ggtagattaa
    21301 ctgctgagga taagaaagaa attgattggg caatggaagt tacaggaact gatacattcc
    21361 gacaccgttc aatcaatgat ttaagtggtg gtcaaagaca acgtgtttgg attgcaatgg
    21421 cattagcaca aagaactgat attatctttt tagacgaacc aacaacatat ttagatatct
    21481 gtcatcaatt agaaatacta gaattagttc agaagctaaa tcaggaacaa ggttgtacaa
    21541 ttgtcatggt tcttcatgat atcaaccaag cgattcgttt ctcagatcat cttattgcga
    21601 tgaaagaagg ggatatcatc gctacaggtt caacagaaga cgtattaaca caggaaatat
    21661 tagaaaaagt ttttaatatt gatgttgttt taagtaaaga tcctaaaact ggaaaacctt
    21721 tactggtaac ttatgactta tgtcgcagag cttattctta attaagtaag ttaatatgat
    21781 aaaaaggaca attaacatga caaatagaga gaacccaacg ccattgaagt ttttatccta
    21841 tattataggt ttaagtatga tactactaat cacactattt atttctacat taataggtga
    21901 cgccaaaatt caagcctcta caattataga ggctattttt aattataatc ctagcaatca
    21961 acagcaaaac atcatcaatg agattaggat tcccagaaat atagcagcag taattgtagg
    22021 tatggcgctt gcagtttctg gtgcgattat acaaggtgtt actcgtaatg gtcttgctga
    22081 tccggcgctc ataggtttaa attcaggtgc ttcatttgct ttagcattaa catatgcagt
    22141 tttaccaaac acttcatttt taatattgat gtttgctgga tttttaggtg ctattctagg
    22201 aggtgctatt gtattaatga taggccgatc tagacgtgat ggatttaatc cgatgcgtat
    22261 tattttagcg ggtgcagcag taagtgctat gttaacagcg ctaagtcaag gtattgcatt
    22321 agcttttaga ctaaatcaaa cagtaacatt ttggactgct ggaggcgttt caggcacaac
    22381 atggtcacac cttaagtggg caattccatt aattggtatt gcgttattca ttatattaac
    22441 aattagtaaa caacttacca ttttaaatct tggtgaatca ttagctaaag gtttaggtca
    22501 aaatgtaaca atgatcagag gcatatgttt aattattgct atgattctag caggtattgc
    22561 agttgctatc gctggacaag ttgcatttgt aggtttgatg gtacctcata tagcaagatt
    22621 tttaattgga actgattatg ctaaaattct accattaaca gccttgttag gtgggatact
    22681 cgtgcttgtt gccgatgtga tagcacgata tttaggagaa gcgcctgttg gtgcaatcat
    22741 ttcatttatc ggtgttcctt actttttata tttagttaaa aaaggaggac gctcaatatg
    22801 attagttcaa ataataaacg cagacaattg atagcactgg ctgtttttag cattctacta
    22861 tttctaggtt gtacttggag tattacctca ggtgaataca acatacctgt tgaaagattt
    22921 ttcaaaactt taattggaca aggtgatgcc attgataagt taatcttatt agatttcagg
    22981 ttacctcgga tgatgattac tattttggct ggcgcagcgc ttagtattag tggtgcaata
    23041 gtgcaaagtg tcacaaaaaa tccaatagct gaaccaggta tattaggtat taacgcaggt
    23101 ggcggatttg caatcgcatt atttattgca attggtaaaa ttaatgctga caactttgtt
    23161 tatgtactgc cgttaataag tatactaggt ggtatcacca ctgcattgat tatttttatt
    23221 ttcagtttta ataaaaatga aggtgttaca cctgcgagta tggtattaat aggtgtaggt
    23281 ttacaaacag cattatatgg tggctcaatt acaattatgt caaaatttga tgataagcaa
    23341 tctgatttca tcgctgcttg gtttgcaggt aatatttggg gtgacgaatg gccatttgtc
    23401 attgcatttt taccgtgggt gttgattatt attccttact tactatttaa atcgaataca
    23461 ctaaatatta ttcatacggg tgataatatt gcacgaggtc taggtgtaag gttaagcaga
    23521 gaacgtttaa tattattctt tatcgcagtg atgttatcat ctgctgctgt agcagtagca
    23581 ggttcaattt cgtttatcgg attaatgggt ccgcatattg ccaaacgtat cgttggacca
    23641 cgtcaccagt tgtttttacc aattgccatt ttagtagggg catgtttact tgttatagct
    23701 gatacaattg gcaaaattgt attacaacca ggtggggttc cagcaggtat tgtcgtagca
    23761 attattggtg caccgtattt cttatattta atgtacaaaa cgaaaaatgt atagtgtcaa
    23821 tggacacaac ttattgctat gaaaggcact ttattataag gcttttcata gcatttttta
    23881 tttaatgagc cactcaagac tatttatttt ttcaataatg aaccattaag ttatcaagag
    23941 gatcttatca aaaatatatt tgataacggt atcaggttaa ttctttatga tagcgcattc
    24001 atttattctg ttttatacta tgactgataa taccaaggag gtacaacatg atgaaaaagt
    24061 taatcaataa aaaagaaaca tttttaactg atatgcttga aggattgtta attgcgcacc
    24121 cagagttaga tctgattgct aatacagtta ttgtaaaaaa agctaagaaa gaacatggtg
    24181 tagcaatagt ctctggaggt ggaagcggac atgaacctgc gcatgccggt tttgttgcag
    24241 aaggtatgct agatgcagcg gtttgtggcg aagtatttac atcacctaca cctgataaaa
    24301 tattagaagc tattaaagca gtagatactg gtgatggtgt attactagtt gtaaaaaact
    24361 atgcaggtga cgtgatgaat ttcgaaatgg cacaagagct tgcagaaatg gaaggtataa
    24421 atgttcaaac tgttattgtt cgtgacgaca ttgctgtgac aaacgaagta caacgtcgtg
    24481 gtgttgcagg aacagtgttt gttcataagc ttgccggtta tcttgctgaa aaaggttatt
    24541 cattaacaga gataaaatcg cgtgtagaag cgttgttacc tgaaattaaa agtattggta
    24601 tggcaattga gccaccgctt gttccaacta ctggaaaata tggctttgat attgaagacg
    24661 acaaaatgga aatcggtatt ggtatacatg gtgaaaaagg tattcatagg gaagaagtaa
    24721 aggatattga tcatattgtt ggaacattgt tagacgaatt gtataaagaa gttactgcca
    24781 atgatgtcat attaatggta aatggtatgg gtggtacgcc gttatctgaa ttaaatatcg
    24841 taactaaata tattcaacaa aatttagctg caagaacggt taatgttgct aaatggtttg
    24901 ttggtgatta tatgacatct ttagacatgc aaggtttttc tataactatc gtgcctaata
    24961 aaccagaata tttggaagca tttttagcac caacaacaag tcaatacttt aaataagaag
    25021 tgaatatgaa taaatataca tttatgaggt ggcacaaatg aaagtgaatg atatgaaagc
    25081 acgtttatta aatttagaag aaacgtttaa aaaacatgaa tctgaattaa ctgaattaga
    25141 tcgagcaatt ggtgatggtg accacggggt taacatggtt cgtgggttta gtagtcttaa
    25201 agacaaactt gatgatagct caatgcaatc attgttcaaa tcaactggta tggcattgat
    25261 gtcaaatgtt gggggtgcat caggaccact gtatggcttt agctttgtta aaatgtctgc
    25321 agtcaccaaa gatgatatgg ataatcaaga tttcattaca ctaattcagg catttgccga
    25381 agcggttgaa tcacgtggta aagttacttt aaatgaaaag acaatgtatg atgtagtagc
    25441 gcgagcagca gagaagctta aaaatggtga aactttaaca ttcaatgatt tacagcaatt
    25501 agcagataat acaaaagata tggtagcaac gaaaggtaga gctgcatatt ttggagaaga
    25561 atcaaaaggt tatattgatc caggtgctca aagtatggtt tatattttaa acgctttgat
    25621 tggagatgaa gataatgcct aaaattatac ttgttagcca cagtaaagaa attgcaagtg
    25681 gtacaaaatc tttgttaaag caaatggcag gtgacgttga tattatacca atcgggggat
    25741 taccagatgg ttcaattgga acttcatttg atatcatcca agaagttttg actaaattag
    25801 aggatgatgc attgtgtttt tacgatattg gatcttcaga aatgaatgta gatatggcaa
    25861 ttgaaatgta tgatggtaat catcgtgtgt taaaagttga tgcaccaatt gttgaaggca
    25921 gttttatcgc agcagtaaag ctatcaatcg gcggttcaat tgatgatgca ttagcagaaa
    25981 tcaaacaatc attttagtta aaatttacta ataatgaaaa atgtaaacct ttttcaaatg
    26041 aaactttata aaaaatatga tagtatatat gtaaatgttt aataaaatct ggagaaatag
    26101 gaggacattg ccatgcaaca ccttataaaa aaacatgtat tgaatggcga gtttgattta
    26161 gtacgacaat tgatgtccga aacagatttt atggaatttg aagaagcata tatttcaagt
    26221 gcgcatgaag tagaaagtat gatgttttat acatgtattt tagatatgat taagtacgaa
    26281 gaatcatctg aaatgcatga cttagcattt ttattgcttg tgtatccact aagtgaatat
    26341 gaaggtgctt tggattctgc ttattatcat gcagacgctt ccataaaact tactgacggc
    26401 aaagaagtta aaagtttgtt acaaatgtta ttattgcatg cgataccaac acctgttatt
    26461 tcagataaga aggcttttga tatcgccaag caaattttaa aattagatcc taataataat
    26521 gttgctcgta acgtcttaaa agacactgcc aaacgtatgg acaacgttgt tgttgatata
    26581 aatgaattac accaacgtaa tgcacgttaa ttacatttca attatattag cttaataata
    26641 gttttaacat ttggttgggt tgggcatatg ttccagcctt ttttaatact taaaaactaa
    26701 cgaagtatac ttgtgtgcac aaatggtttt tatacaacat tttataaatt tatacatttt
    26761 aataaagaac atacgataga tggtttaaac cttgttaact gagaaatttt gatatgtatt
    26821 cttcgaaatt taactaaata tacgaaattc aagaagcaca ataattaatc atttttccta
    26881 tacaaaagtt cgtatgactg cattataaaa gcataaattt ataatttttt ttaaatgtca
    26941 ttgaacgtga taatgtgaat ggattgagca attttgaaaa agtgaaaaat aacctatgcg
    27001 gcttgcaatt aattttcagt acgttataat gcacactgtg caaaattaag gaggtctatt
    27061 attcacatga tgatgaataa agaagcaaca aaaattggat ttgcctacgt cggcattgta
    27121 gtgggcgcag gattttcaac tggacaagaa gttatgcaat ttttcactaa atatggcttg
    27181 tgggcttatt taggtgttat tatatctggt tttattttag cttttattgg gcgccaagta
    27241 gcaaaaattg gtactgcctt tgaagcgaca aatcatgaat caacattaca atacgtattc
    27301 ggtgaaaagt ttagtaaagt ctttgattat attttaatct tcttcttatt tggtatagct
    27361 gtaaccatga tagctggtgc aggcgcaaca tttgaagaaa gttataacat acctacatgg
    27421 ctaggtgctt taattatgac attagcgatt tatattacgt tgctattaga ctttaataaa
    27481 atagtacgtg cactaggtat cgttacacca tttttaattg ttttagttgt attaatcgct
    27541 ggcgtttatt tatttaaagg tcatgtttca ttagcagaag ttaaccaagt agtgcctgaa
    27601 gcaagtattt ggaagggaat ctggtttggt acaatatatg gtggattagc tttttctgta
    27661 ggttttagta ccatcgtagc aatcggtggg gatactgaaa agcgtacagt gtcaggtgca
    27721 ggcgcgatgt atggtggtat tatctatact gtattactag cattgatcaa ctttgcattg
    27781 caaagtgaat atccaactat taaaaatgcc tcaattccta cattgacgtt agcaaataat
    27841 atccatcctt taatagcaac agtgttatct gttattatgc tggcggttat gtataatact
    27901 attctaggac taatgtattc atttgcagca cgttttacag aaccatacag taaaaattat
    27961 catatcttta ttattataat gatggtagca ggttatttat taagtttcgt aggatttgct
    28021 gaattaatta ataagttata tacaattatg ggatatgtag gcttatttat tgtagtagct
    28081 gtaattatta aatatttcaa acgtaaaaat gcggataaaa aacatattgc ttaatatcat
    28141 atgagggata tccgaaactt tacaattgaa tcactttgtt ttaaccttaa aagcaattcg
    28201 tctctactct tatcgggcga attgcttttt atatttattc agtctattaa tatgagcgtc
    28261 taacaaatag agaggtacga tgtaatgaat aaagataata aatggacgat gataactgcg
    28321 ctttttataa ctgtaatcag tgtattgtta gcatttcatc tgaaacaaca ttatgaccaa
    28381 attacaaatg agaaccatgc taataaagac aaaattaata ttaaaaataa aaatgtgcgc
    28441 atttatcaaa accttacata caatagagtt ttccctaaca gtaaattaga tattattaca
    28501 cctgttgata tgtcttctaa tgccaaactg ccagttattt tttggatgca cggtggtggt
    28561 tatattgcgg gtgataagca gtataaaaac ccattattag cgaaaattgc tgaacaaggg
    28621 tacattgttg tgaatgtaaa ttatgcattg gcgccacaat ataaatatcc cacaccatta
    28681 attcaaatga atcaagcaac tcaattcatt aaagaaaata aaatgaattt acctattgat
    28741 tttaatcaag taattattgg cggtgattct gcaggtgctc aattagctag ccaatttacg
    28801 gcaatacaga cgaatgatcg cttaagagaa gccatgaaat ttgatcagtc attcaaacca
    28861 tcgcaaatta aaggtgctat actatttggt ggtttttata atatgcaaac agttagagaa
    28921 actgagtttc caagaataca gttatttatg aaaagttata ctggcgaaga agattgggaa
    28981 aagagtttta aaaacatttc acaaatgtcg acagtaaaac aatcgacaaa aaattatcca
    29041 ccaacatttt tatctgttgg agatagcgat ccattcgaaa gtcaaaatat agaattcagt
    29101 aagaaattac aagaattgaa tgtaccagta gatactttgt tttatgatgg tacgcatcat
    29161 ttacatcatc agtatcaatt tcaccttaat aaacctgaat cgatagataa tatcaaaaaa
    29221 gtgttacttt tcttaagtcg taatacatcc tctagtggta ttcaaactga agagaaacca
    29281 caaatagaaa atccgagtaa tgaattaccg ttaaatcctt taaactaatg ataaacagta
    29341 gtaatttatt gcttaagcaa catttaagat tttcaaatta aaaacgaaga atttaaaaca
    29401 tgtggtgcta atgtgtaaga gtctgagata taataaattg ttagcggttc tttatcattt
    29461 ctatctcact ctattatgat gtgacattta ttttacaaac taatttgttt tgaacctgaa
    29521 aataactttt tacaactaaa ttgtcgaaaa acagtgtgta catgatataa taaatttata
    29581 aattgaaaag aattcaaaag aaaaattaaa atatagattg agcacgaagt gatttgaaat
    29641 aaggttgtga aagggaatga caaggtcagc attaaaacca tttaaaaata aacgcgttat
    29701 ggttactgga cgtatacaac gtgttttgtt taaaaattat ttagatagac atagcacatt
    29761 taagccgaat gtaaggatat tattaaaaga tgtatttgtt tcaggtgtat caatagatca
    29821 tttatggtta tatgagacaa ataaatacta tgcattggca atggaactta ttcatcaacg
    29881 agtaaaattt agtgcgaatg ttgtaccgta ttacaaaata aatagaaata ataatttatt
    29941 cgtacaagat tatggaatta agcgtaaagg taggttaatt actgaagaag cttacaatca
    30001 aaacaatcag tatcaggata agatatatga aaaattaccg gatatagatt ttagactcga
    30061 agatttttat agtaaggaaa actaaacaac atatataaag caccactaat cgtagttgat
    30121 agttaatcaa ttacacatta gtggtgtttt attttgaatt taaatttgat aaatgaataa
    30181 gagtgtacgt atttatttga gattagataa cattgataag tatgagtaat gtattaggta
    30241 accattaaat tcgttgttat acgattctga tacaagatta tgataaaata gctttaatta
    30301 agaattttag ccatcatata gtcatcataa tatttaccat cgataaataa cttatctttt
    30361 aaaacgcctt cgatttgaaa atcggcactt ttaaaaagct cgagggcagg ttggttattg
    30421 agtggtacat ttgcttcaat tcggtgtatt tgattgttta aacaccaagc cataatggca
    30481 tcaagaagtg cttggccaat tccacgatgt tgatataatt tctttacacc taaatcaatt
    30541 ttagcaacat gtttaatgcg ttgaaatggt gtcgtattaa caaaggcaaa gccaacgagt
    30601 tgttcatcac tttcagcaac gaagatgact ttatgcggag aagtgatata ttcttctaat
    30661 tgtttactag ccgatgtgac gctaggatca tattctcctg gtgtgtagaa catatacgga
    30721 gattcgtcgt atatgttcgc taacattgaa atgaaatttt ctacatcttt gatactaact
    30781 ctacgtataa tatgggccat aaaaagcctc cagtattttg aaattttaaa acatttgcta
    30841 ctattataat atatatgtaa ctaaaaggtg gagtaatatg ttttttggtt acagtttaaa
    30901 ggagtatttt agatgaaacc taaagtttta ttagcaggtg gaacaggata tattggtaag
    30961 tatttaagtg aagtgattga aaatgatgct gaactttttg ctatatcaaa atatccagac
    31021 aataaaaaaa cagatgatgt tgaaatgact tggattcagt gtgatatatt tcattacgaa
    31081 caggttgttg cagcaatgaa tcaaatagat attgctgtat tctttatcga cccaacaaag
    31141 aattctgcca aaataacaca atcatcagca agagatttaa cattaatcgc agcagataat
    31201 tttggtcgag cagcggctat caatcaagta aaaaaagtaa tctacatacc tgggagtcgt
    31261 tatgataatg aaacaattga acgcctaggt gcatatggta cacctgtaga aacaacaaat
    31321 ttagttttta aacgttcttt agttaatgta gaattacaag tttcaaagta tgatgatgtt
    31381 agatcaacga tgaaggtagt tttaccaaag ggatggacat taaagaacgt tgtaaaccat
    31441 tttattgcat ggatgggtta cactaaagga acttttgtga aaacagaaaa atcacatgat
    31501 caatttaaga tatatattaa gaataaggtg cgaccgctcg cagtatttaa aatagaagaa
    31561 acagctgatg gaataataac tttaatttta ttgagtggaa gtttagtgaa aaaatataca
    31621 gttaatcaag ggaagttaga atttagatta atcaaagagt cggcagtcgt ttatatacat
    31681 ctatacgatt atatccctcg attattttgg ccgatttatt actttataca agcaccaatg
    31741 caaaaaatga tgattcatgg ctttgaagtt gactgccgga ttaaagattt tcaaagtcga
    31801 ttaaaatcag gagaaaatat gaaatatact aaatgatatt gggtgatatg gatgcaaata
    31861 ctactagtag aagatgacaa tactttgttt caagaattga aaaaagaatt agaacaatgg
    31921 gattttaatg ttgctggtat tgaagatttc ggcaaagtaa tggatacatt tgaaagtttt
    31981 aatcctgaaa ttgttatatt ggatgttcaa ttacctaaat atgatgggtt ttattggtgc
    32041 agaaaaatga gagaagtttc caacgtacca atattatttt tatcatctcg tgataatcca
    32101 atggatcaag tgatgagtat ggaacttggc gcagatgatt atatgcaaaa accgttctat
    32161 accaatgtat taattgctaa attacaagcg atttatcgtc gtgtctatga gtttacagct
    32221 gaagaaaaac gtacattgac ttggcaagat gctgtcgttg atctatcaaa agatagtata
    32281 caaaaaggtg atcagacgat tttcctgtcc aaaacagaaa tgattatatt agaaattctt
    32341 attaccaaaa aaaatcaaat cgtttcgaga gatacaatta tcactgcatt atgggatgat
    32401 gaagcatttg ttagtgataa tacgttaaca gtaaatgtga atcgtttacg aaaaaaatta
    32461 tctgaaatta gtatggatag tgcaatcgaa acaaaagtag gaaaaggata tatggctcat
    32521 gaataatttg aaatgggtag cttatttttt gaaatctcgc atgaactgga tattttggat
    32581 attgttttta aacttcctta tgttaggcat tagtctaatc gattatgatt ttccaataga
    32641 cagtttattt tatattgttt ctttgaattt aagtttaaca atgatttttc ttttattgac
    32701 atattttaaa gaagtaaaat tatataagca ttttgacaaa gataaagaaa tagaagaaat
    32761 taaacataaa gatttagcgg aaacgccatt tcaacgtcat acagttgatt atttatatcg
    32821 tcaaatctca gcgcacaaag aaaaggttgt tgagcaacag ttgcaattga acatgcatga
    32881 acaaaccatt acagaatttg tgcacgacat aaaaacacct gtgacagcta tgaaattatt
    32941 aattgatcaa gaaaaaaatc aagaaagaaa acaagcatta ctatatgaat ggtctcgtat
    33001 aaactcgatg ctagatacac agctgtatat tactagatta gaatctcaac gtaaagatat
    33061 gtattttgat tacgtgtcac ttaaacgcat ggtcattgat gaaatacaat taacaagaca
    33121 tattagtcag gttaaaggta ttggttttga tgttgacttt aaagtggatg attatgttta
    33181 tacagatata aaatggtgtc gtatgattat tagacaaatt ttgtcaaacg cattgaaata
    33241 tagtgagaat tttaatattg aaattgggac agaattaaat gatcaacatg tttcgttata
    33301 tattaaagac tatggcagag gtattagtaa aaaagatatg ccgcgaatat ttgaacgagg
    33361 atttacgtca acggctaaca gaaatgaaac gacgtcttca ggtatgggtc tatatttagt
    33421 aaatagtgta aaggatcaat taggtattca cctgcaagtc acgtcgactg ttggtaaggg
    33481 gacaactgtc agattgattt tcccattaca aaatgaaatt gttgaacgca tgtcggaagt
    33541 gacaaatttg tcattttaaa catgcgtttt gttacttaga attgatacat caatgcagct
    33601 tcaacgttat aataagatag atgttagtca tatgttaaat tgaagataca agtgccaaag
    33661 cctaaaggaa atgaagttaa gataaattat aggagtgtta aagtggcaat tttagaagta
    33721 aaacaattaa caaaaatata tggaactaaa aaaatggcac aagaagtgtt gcgagatatc
    33781 aatatgtcta ttgaagaagg cgagtttatt gctattatgg gtccctctgg atctgggaaa
    33841 acgacattat taaatgtttt aagttcaatt gattatattt cacaaggttc tattacatta
    33901 aaaggaaaaa aattagaaaa gctttcaaac aaggaattat ctgatatacg caagcatgat
    33961 attggtttta tttttcaaga gtataattta ctgcatacat tgactgttaa agaaaacata
    34021 atgttaccac taacggtaca gaagttagat aaagaacata tgttaaatcg ttatgaaaaa
    34081 gtagcagaag cattaaatat attggatatt agtgataaat atccctctga attgtctggt
    34141 ggacaaaggc aacgaacatc agctgccaga gcatttataa cattgccttc tattatattt
    34201 gctgacgaac caacaggtgc attggattct aaaagtactc aagatttatt aaaacgatta
    34261 acaagaatga atgaagcatt taagtctaca attattatgg taacgcatga tcctgttgca
    34321 gcaagctatg caaatcgagt agtgatgcta aaagatggtc aaattttcac tgaattatac
    34381 caaggggatg acgataaaca tacctttttc aaagaaataa tacgtgtaca aagtgtttta
    34441 ggtggcgtta attatgacct ttaacgagat aatatttaaa aatttccgtc aaaatttatc
    34501 acattatgcc atctatcttt tttcgttaat tacgagtgta gtattgtatt ttagctttgt
    34561 agcattaaaa tacgctcata aactaaacat gacagagtca tatccaatta taaaggaagg
    34621 ctcacaagtc ggaagctact ttctattttt catcataatt gcatttttgt tatatgccaa
    34681 tgtgttattt attaaacgac gaagttatga gcttgcatta tatcaaacat taggtttatc
    34741 taaattcaac attatttata tactaatgct cgaacaatta ctaatattta taattacggc
    34801 aatattaggt attattattg gtatttttgg ttcgaaactg ttattaatga ttgtctttac
    34861 attattagga attaaagaaa aggttccaat tatttttagt ttgagggcgg tatttgaaac
    34921 attaatgtta atcggtgtcg cttatttttt aacatctgct caaaatttta tattagtgtt
    34981 caaacaatct atttcacaga tgtcaaagaa taaccaggtt aaagaaacaa atcataataa
    35041 aattacattt gaagaggttg ttttaggcat cttaggtata gtattgatta ccacaggata
    35101 ctatctatct ttgaacattg ttcaatatta tgattctatc ggtacactta tgtttatttt
    35161 attgtcaact gtgattgggg catacttatt ttttaaaagc tctgtttctc tagtttttaa
    35221 aatggtgaag aagtttagaa aaggtgttat aagtgtaaat gatgtcatgt tctcatcatc
    35281 tattatgtat cgtattaaga aaaatgcttt ttcacttacg gtcatggcaa tcatttcagc
    35341 gattactgtt tcagttcttt gctttgctgc tataagtaga gcgtccttat caagtgaaat
    35401 aaaatatact gcaccacacg acgttacaat taaagaccaa caaaaagcta atcaattagc
    35461 aagtgaatta aacaatcaaa aaattcctca tttttataat tataaagaag taattcatac
    35521 gaaattgtat aaagataatt tatttgatgt aaaagcgaaa gaaccataca atgtaacaat
    35581 tactagtgat aaatacatcc ctaatactga tttgaaacgt gggcaagctg atttatttgt
    35641 agcggaaggt tctatcaaag atttagtgaa acataagaag catggtaagg caattatagg
    35701 aacgaaaaaa catcatgtta atattaagtt acgtaaagat attaataaaa tctattttat
    35761 gacagatgtt gatttaggtg gaccaacgtt tgtcttaaat gacaaagact atcaagaaat
    35821 aagaaagtat acaaaggcaa agcatatcgt ctctcaattt ggattcgatt tgaaacataa
    35881 aaaagatgct ttagcattag aaaaagcgaa aaataaagtt gataaatcta ttgaaacaag
    35941 aagtgaagcg ataagctcaa tatcaagttt aaccggaata ttattatttg taacatcatt
    36001 tttaggtatt acattcttga ttgctgtatg ttgcattata tacataaagc aaatagatga
    36061 aaccgaagat gagttagaga attatagtat tttgagaaag cttggattta cacaaaaaga
    36121 tatggcaagg ggactaaagt ttaaaattat gtttaatttt gggttacctt tagttattgc
    36181 actatcacat gcatatttta catcattagc atatatgaaa ttaatgggta caacgaatca
    36241 aataccggtt ttcatagtaa tgggattata catttgtatg tatgctgttt ttgcagtgac
    36301 ggcttataat cattccaagc gaacaattag acattccata taaaatatac agatggcttt
    36361 cagtagagta gtggattcgg attcacgaac tatactggaa gctttttatt ataaatgaag
    36421 agaagttata tttttagcat gtatagttga atactgggtt aaaataccat attaataatg
    36481 aagtaaaggt atgagtgatt atgaaagtgt tttgaatgaa atatatttaa ttggtgatgc
    36541 ttttaattga aaagattaac aggattcaac tttgtaaatt gtattaaatg tgagaaaata
    36601 aaagtatatt cattgagaga tatatgagtc aatgatcgtt ttaaacaaga taagtgtatt
    36661 ttaatatgta aaagttatgt aataaatatt gtatcgttgc aaatttccca actatatcca
    36721 tttacaattt ttagagttgt atagataaaa aattgcctat acaataactt tttcaaaaca
    36781 aatgcctact actatttaaa ttttatgtaa aagttaagtt tcggaattgt taaaaatttg
    36841 taaattgaat tgtgggattg taaattttgt gctattttca tataagggca aatacagtaa
    36901 attgtttact gtgaggcatt tttgaaatta atatcagtac actaaaatta ta
//
LOCUS       DAMQYT010000011        31542 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMQYT010000011 DAMQYT010000000
VERSION     DAMQYT010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 31542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31542
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.011"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            98..6913
                     /gene="sasA"
                     /locus_tag="PGA33_000441"
     CDS             98..6913
                     /gene="sasA"
                     /locus_tag="PGA33_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HDG6472280.1"
                     /translation="MSKRQKAFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGHGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLNVQPTNNTFQDFDINYNGDTKVMTVKYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPIVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTISIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSDSASKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDS
                     TSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSQSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSAFTSTSGSVSTSTSLSTS
                     NSERTSTSMSDSTSLSTSESDSISESTSTSDSISEAISASESTFISLSESNSTSDSES
                     QSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSTSIS
                     TSTSISESTSTFKSESVSTSLSMSTSTSLSDSTSLSTSLSDSTSDSKSDSLSTSMSTS
                     DSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTS
                     TSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSSYTSQSTSQS
                     ESTSTSTSLSDSTSISKSTSQSGSVSTSASLSGSESESDSQSISTSASESTSESASTS
                     LSDSTSTSNSGSASTSTSLSNSASASESDLSSTSLSDSTSASMQSSESDSQSTSASLS
                     DSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGS
                     ASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDS
                     TSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDS
                     KSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSCSQSMSDSVSTSDSSSLSVSTSLR
                     SSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTS
                     TSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNS
                     QFDSMSISASESDSMSTSDSSSISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGS
                     TSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTS
                     LSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGS
                     TSTSISESLSTSGSGSTSVSDSTSMSESNSSSVSMSQDKSDSTSISDSESVSTSTSTS
                     LSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHTHSTS
                     TSRLSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGGVMTL
                     LVGLGLMKRKKKKDENDQDDSQA"
     gene            7043..8254
                     /gene="secY2"
                     /locus_tag="PGA33_000442"
     CDS             7043..8254
                     /gene="secY2"
                     /locus_tag="PGA33_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HDG6472281.1"
                     /translation="MLKLLQQYEYKIIYKRMLYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKSISDDMPMLTF
                     DSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGLALVTVIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLNQYW"
     gene            8265..9818
                     /gene="asp1"
                     /locus_tag="PGA33_000443"
     CDS             8265..9818
                     /gene="asp1"
                     /locus_tag="PGA33_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HDG6472282.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            9808..11376
                     /gene="asp2"
                     /locus_tag="PGA33_000444"
     CDS             9808..11376
                     /gene="asp2"
                     /locus_tag="PGA33_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HDG6472283.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLT
                     DDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            11354..12313
                     /gene="asp3"
                     /locus_tag="PGA33_000445"
     CDS             11354..12313
                     /gene="asp3"
                     /locus_tag="PGA33_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HDG6472284.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQYDLEYKSMRVTKIG
                     DDQYGNDMINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDNIEGNIKQLKNDLQS
                     QDTLKAINLISYGSKSNVFATYVAQHLGCKVYRTSHEDDDLKGWLTEHVPGNHELKDT
                     NVEVYFKEEQDKHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            12303..14693
                     /gene="secA2"
                     /locus_tag="PGA33_000446"
     CDS             12303..14693
                     /gene="secA2"
                     /locus_tag="PGA33_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HDG6472285.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENQDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            14711..16219
                     /gene="gtfA"
                     /locus_tag="PGA33_000447"
     CDS             14711..16219
                     /gene="gtfA"
                     /locus_tag="PGA33_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HDG6472286.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISLAPTTYTLDDIERELGHDISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDIVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNQDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            16212..17570
                     /gene="gtfB"
                     /locus_tag="PGA33_000448"
     CDS             16212..17570
                     /gene="gtfB"
                     /locus_tag="PGA33_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HDG6472287.1"
                     /translation="MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFALFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            17812..19719
                     /gene="sasF"
                     /locus_tag="PGA33_000449"
     CDS             17812..19719
                     /gene="sasF"
                     /locus_tag="PGA33_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HDG6472288.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTE
                     ITKEILSKQDLLDKVDKAIRQIEQLKQLSASSKEHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDASKDTSEDPAVSTTDNNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEAVKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSDLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            19887..20447
                     /locus_tag="PGA33_000450"
     CDS             19887..20447
                     /locus_tag="PGA33_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDG6472289.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(20714..22573)
                     /locus_tag="PGA33_000451"
     CDS             complement(20714..22573)
                     /locus_tag="PGA33_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDG6472290.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDIDKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKAQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            22784..25765
                     /locus_tag="PGA33_000452"
     CDS             22784..25765
                     /locus_tag="PGA33_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDG6472291.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFVDITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFSKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            complement(25877..26815)
                     /gene="manA"
                     /locus_tag="PGA33_000453"
     CDS             complement(25877..26815)
                     /gene="manA"
                     /locus_tag="PGA33_000453"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDG6472292.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(26829..28781)
                     /locus_tag="PGA33_000454"
     CDS             complement(26829..28781)
                     /locus_tag="PGA33_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDG6472293.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDNTAKHSHLNKPSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMGSTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(28858..30732)
                     /locus_tag="PGA33_000455"
     CDS             complement(28858..30732)
                     /locus_tag="PGA33_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDG6472294.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAKHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVAPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            complement(30972..31430)
                     /locus_tag="PGA33_000456"
     CDS             complement(30972..31430)
                     /locus_tag="PGA33_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472295.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKSTHK
                     KTETEENDQQDDSADKTDKNQNNGNNVTNDNQPSSPSNVPNNHSTVPSNTQPAQPKDS
                     NSNKNNTHGGGSQSSTNNQNNQQQNTPPANKSNNTNTPSKQTPQAQSPKN"
BASE COUNT        10892 a         5014 c         5896 g         9740 t
ORIGIN      
        1 aaatgagtat ttttgtatca aaaaagagtt gtattaaatt gaatagtggc atatattata
       61 atctaatgaa tgtaaagata atacaaggag ttattacatg agtaaaagac agaaagcatt
      121 tcatgacagc ttagcaaacg aaaaaacaag agtaagactt tataaatctg gaaaaaattg
      181 ggtaaaatcc ggaattaaag aaatagaaat gttcaaaatt atggggctac catttattag
      241 tcatagttta gtgagtcaag ataatcaaag cattagtaaa aaaatgacgg gatacggact
      301 gaaaactacg gcggttattg gtggtgcatt cacggtaaat atgttgcatg accagcaagc
      361 ttttgcggct tctgatgcac cattaacttc tgaattaaac acacaaagtg aaacagtagg
      421 taatcaaaac tcaacgacaa tcgaagcatc aacatcaaca gccgattcca caagtgtaac
      481 gaaaaatagt agttcggtac aaacatcaaa tagtgacaca gtctcaagtg aaaagtctga
      541 aaaggtcact tcgacaacta atagtacaag caatcaacaa gagaaattga catctacatc
      601 agaatcaaca tcctcaaaga atactacatc aagttctgat actaaatctg tagcttcaac
      661 ttcaagtaca gaacaaccaa ttaatacatc aacaaatcaa agtactgcat caaataacac
      721 ttcacaaagc acaacgccat cttcggtcaa cttaaacaaa actagcacaa cgtcaactag
      781 caccgcacca gtaaaacttc gaactttcag tcgcttagct atgtcaacat ttgcgtcagc
      841 agcgacgaca accgcagtaa ctgctaatac aattacagtt aataaagata acttaaaaca
      901 atatatgaca acgtcaggta atgctaccta tgatcaaagt accggtattg tgacgttaac
      961 acaggatgca tacagccaaa aaggtgctat tacattagga acacgtattg actctaataa
     1021 gagttttcat ttttctggaa aagtaaattt aggtaacaaa tatgaagggc atggaaatgg
     1081 tggagatggt atcggttttg ccttttcacc aggtgtatta ggtgaaacag ggttaaacgg
     1141 tgccgcagta ggtattggtg gcttaagtaa cgcatttggc ttcaaattgg atacgtatca
     1201 caatacatct aaaccaaatt cagctgcaaa ggcgaatgct gacccatcta atgtagctgg
     1261 tggaggtgcg tttggtgcat ttgtaacaac agatagttat ggtgttgcga caacgtatac
     1321 atcaagttca acagctgata atgctgcgaa gttaaatgtt caacctacaa ataacacgtt
     1381 ccaagatttt gatattaact ataatggtga tacaaaggtt atgactgtca aatatgcagg
     1441 tcaaacatgg acacgtaata tttcagattg gattgcgaaa agtggtacga ccaacttttc
     1501 attatcaatg acagcctcaa caggtggcgc gacaaattta caacaagtac aatttggaac
     1561 attcgaatat acagagtctg ctgttacaca agtgagatac gttgatgtaa caacaggtaa
     1621 agatattatt ccaccaaaaa catattcagg aaatgttgat caagtcgtga caatcgataa
     1681 tcagcaatct gcattgactg ctaaaggata taactacacg tccgtcgata gttcatatgc
     1741 gtcaacttat aatgatacaa ataaaactgt aaaaatgacg aatgctggac aatcagtgac
     1801 atattatttt actgatgtaa aagcaccaac tgtaactgta ggcaatcaaa ccatagaagt
     1861 gggtaaaaca atgaatccta ttgtattgac tacaacggat aatggtactg ggactgtgac
     1921 aaatacagtt acaggattac caagcggatt aagttacgat agtgcaacga attcaatcat
     1981 tgggacacca acaaaaattg gtcaatcaac agtgacagtt gtgtctactg accaagcaaa
     2041 taacaaatcg acgacaactt ttacaataaa tgttgtggat acgacagcac caacagtgac
     2101 accaatagga gatcaatcat cagaagtgta ttcaccaata tccccgatta aaattgctac
     2161 gcaagataac agtggaaatg cggtgacgaa tacagtgact ggattgccat ccggactaac
     2221 atttgatagt acaaataata ctattagtgg tacaccaaca aacattggta caagtactat
     2281 atcaatcgtt tctacagatg cgagcggtaa caaaacgacg acaactttta aatatgaagt
     2341 aacaagaaat agcatgagtg attccgtatc aacatcagga agtacacaac aatctcaaag
     2401 tgtgtcaaca agtaaagctg actcacaaag tgcatcaacg agtacatcag gatcgattgt
     2461 ggtatctaca tcagctagta cctcgaaatc gacaagtgta agcctatctg attctgtgag
     2521 tgcatctaag tcattaagca catctgaaag taatagtgta tcaagctcaa caagcacaag
     2581 tttagtgaat tcacaaagtg tatcatcaag catgtcggat tcagctagta aatcaacatc
     2641 attaagcgat tctatttcaa actctagcag tactgaaaaa tccgaaagtc tatcaacaag
     2701 tacatctgat tcattgcgta catcaacatc actcagtgac tcattaagta tgagtacatc
     2761 aggaagcttg tctaagtcac aaagcttatc aacgagtata tcagggtcgt ctagtacatc
     2821 agcatcatta agtgacagta catcgaatgc aattagtaca tcaacatcat tgagcgagtc
     2881 agctagcacc tcggactcta tcagtatttc aaatagcata gccaactctc aaagtgcgtc
     2941 aacaagcaaa tcagattcac aaagtacatc aatatcatta agtacaagtg attcaaaatc
     3001 gatgagtaca tcagaatcat tgagcgattc gacgagcaca agtggttctg tttctggatc
     3061 actaagcata gcagcatcac aaagtgtctc aacaagtaca tcagactcga tgagtacttc
     3121 agagatagta agtgactcta tcagtacaag tgggtcatta tctgcatcag acagtaaatc
     3181 aatgtccgta agtagttcaa tgagcacgtc tcagtcaggt agtacatcag aatcattaag
     3241 tgattcacaa agtacatctg attctgatag taagtcatta tcacaaagta ctagtcaatc
     3301 aggttcaaca agtacatcaa cgtcgacaag tgcttcagta cgtacttcgg aatcacaaag
     3361 tacgtctggt tcaatgagtg caagtcaatc cgattcaatg agcatatcaa cgtcgtttag
     3421 tgattcaacg agtgatagca aatcagcatc aactgcatca agtgaatcaa tatcacaaag
     3481 tgcttttacg agcacatctg gttcggtaag tacttcgaca tcgttaagta caagtaattc
     3541 agaacgtaca tcaacatcta tgagtgattc cacaagctta agtacatcag agtctgattc
     3601 aataagtgaa tcaacgtcaa cgagcgactc tataagtgaa gcaatatctg cttcagagag
     3661 cacgtttata tcattaagtg aatcaaatag tactagcgat tcagaatcac aaagtgcatc
     3721 tgccttttta agtgaatcat taagtgaaag tacgtctgaa tcaacatcag agtcagtgag
     3781 tagttcgaca agtgagagta cgtcattatc agacagtaca tcagaatctg gtagcacatc
     3841 aacatcatta agtaattcaa caagtggtag tacgtccatt tcaacatcga caagtatcag
     3901 tgaatcaacg tcaacgttta agagcgagag tgtttcaaca tcactgagta tgtcaacgag
     3961 tacaagtttg tctgactcta caagtttgtc aacatcatta agtgattcca caagtgatag
     4021 taagtctgat tcattaagta catcaatgtc gacaagtgat tcaatcagta caagtaaatc
     4081 tgattccatt agtacatcca catcattaag tggttctaca agtgaaagtg aatccgactc
     4141 aacatcatca agtgaaagta aatccgattc aacatcaatg agcataagta tgtctcaatc
     4201 aacatcagga agtacaagta cgtcaacgag tacaagtttg tctgactcaa cgagtacatc
     4261 attgtcacta agtgcctcaa tgaatcaaag cggagtagac tcaaactcag caagccaaag
     4321 tgcctcaaac tcaacaagta caagcacgag cgaatccgat tcacaaagca catcatcata
     4381 tacaagtcag tcaacaagcc aaagtgaatc cacatcgaca tcaacgtcac taagcgattc
     4441 aacaagtata tctaaaagta cgagtcaatc aggttcggta agcacatcag cgtcattaag
     4501 tggttcagag agtgaatctg attcacaaag tatctcaaca agtgcaagtg agtcaacatc
     4561 agaaagtgcg tcaacatcac tcagtgactc aacaagtaca agtaactcag gatcagcaag
     4621 tacgtcaaca tcgctcagta actcagcaag cgcaagtgaa tccgatttgt cgtcaacatc
     4681 tttaagtgat tcaacatctg cgtcaatgca aagcagtgaa tccgattcac aaagcacatc
     4741 agcatcatta agtgattcgc taagtacatc aacttcaaac cgcatgtcga ccattgcaag
     4801 tttatctaca tcggtaagta catcagagtc tggctcaaca tcagaaagta caagtgaatc
     4861 cgattcaaca tcaacatcat taagcgattc acaaagcaca tcaagaagta caagtgcatc
     4921 aggatcagca agtacatcaa catcaacaag tgactctcgt agtacatcag cttcaactag
     4981 tacttcgatg cgtacaagta ctagtgattc acaaagtatg tcgctttcga caagtacatc
     5041 aacaagtatg agtgattcaa cgtcattatc tgatagtgtt agtgattcaa catcagactc
     5101 aacaagtgcg agtacatctg gttcgatgag tgtgtctata tcgttaagtg attcgacaag
     5161 tacatcaaca tcggctagtg aagtaatgag cgcaagcata tctgattcac aaagtatgtc
     5221 agaatctgta aatgattcag aaagtgtaag tgaatctaat tctgaaagtg actctaaatc
     5281 gatgagtggc tcaacaagtg tcagtgattc tggctcattg agcgtctcaa cgtcattaag
     5341 aaaatcagaa agtgtaagcg agtcaagttc attgagttgc tcacaatcga tgagcgattc
     5401 agtaagcaca agcgattcgt catcattaag tgtatcgacg tcactaagaa gttcagaaag
     5461 cgtgagtgaa tctgattcat taagtgattc aaaatcaaca agtggttcga cttcaacaag
     5521 tacatctggt tcattgagta cctcaacatc attaagtggt tcagaaagcg taagcgagtc
     5581 tacctcgcta agtgattcaa tatcaatgag tgattctact agtacaagtg actccgactc
     5641 attaagtgga tcaatatctt taagtggttc cacaagtctt agcacttcgg attcattaag
     5701 tgattcaaaa tcattgagta gctcgcaaag tatgagtgga tcagaatcaa cgtcaacaag
     5761 tgtgagcgat tcgcagtcaa gctcaacaag taatagtcaa tttgactcta tgagcatcag
     5821 tgcatcagaa agcgactcaa tgtctacaag tgattcgtct agcatcagtg gatcaaattc
     5881 aacgagtaca tcactttcaa catctgactc aatgagcgga agcgtatcag tttcaacatc
     5941 gacaagttta agtgactcaa tatcaggttc aacaagtgta agtgactcga gctcaacaag
     6001 cacatctaca tcattaagtg attcaatgtc acaaagccag tcaacaagta caagtgcatc
     6061 tggttcctta agtacatcga tatcaacatc aatgtcaatg agtgctagta catcgtcatc
     6121 acaaagcaca tcggtgtcga catcattatc aacatcagac agtatcagtg attctacttc
     6181 aataagtatc agtggttcac aaagtacagt agaatcagaa tctacaagtg attcaacttc
     6241 tatcagtgac tcagaatcat tgagtacatc agattcagac tcgacatcga caagtacatc
     6301 ggactcaaca agtggttcaa cttcaacaag catatctgaa tcattaagta cgtctggttc
     6361 aggttcaacg agcgtatctg actcaacatc aatgagtgaa tctaattcat cgagtgtttc
     6421 aatgtcacaa gacaaatccg actcaacatc aattagtgac tcagaatcag tgtcaacaag
     6481 cacatcaacg tcattgagca catccgattc gacaagcaca tccgaatcac tgagtacatc
     6541 tatgtctggt tcacaaagca tttctgactc aacatcaaca agtatgtccg gctcaacaag
     6601 tacatccgaa tctaactcaa tgcatccgtc agactcaatg agtatgcatc atactcacag
     6661 cacgagcaca tctcgcttat caagtgaagc aacaacgagc acgagtgaat ctcagtctac
     6721 attaagtgca acatctgaag tgactaaaca taatggcaca ccagcacaaa gtgaaaaaag
     6781 attgccagat acaggtgact caataaaaca aaatggatta ctaggtggcg ttatgacatt
     6841 attagttggt ttaggtttaa tgaagagaaa gaaaaagaaa gatgaaaatg atcaagatga
     6901 ttctcaagca taaattgaat ttatagctgg ctaaaattaa aaagactgag gcaccaatca
     6961 ctagaaaaat cgatatagga ttttaataat tagtgatgaa cccttagtct ttttaatgtt
     7021 tttgatataa aagaggtgca acatgttaaa acttttacaa caatacgaat ataaaattat
     7081 atataaacgt atgctataca catgttttat cttgtttatt tatatattag gcacaaacat
     7141 ttccatcgtt tcctataacg atatgcaagt aaaacatgag tcatttttta aaatagctat
     7201 ttctaatatg ggtggagacg ttaatacttt aaacattttc actttaggat taggtccgtg
     7261 gttaacatca atgattatct tgatgctgat ttcttatcgg aatatggata aatacatgaa
     7321 acaaacaagt cttgagaagc attataaaga gcgcatctta acattaattt taagtgttat
     7381 tcaaagttat tttgtgattc atgaatatgt ttcgaaagaa cgtgttcatc aagacaatat
     7441 ttacttaaca atattgattt tggttactgg aacgatgtta ttagtttggt tagctgacaa
     7501 aaatagtcgc tacggcatcg caggaccaat gccgattgta atggttagta ttattaaatc
     7561 aatgatgcat caaaaaatgg aatatataga tgcaagtcat attgtgattg cgttgttaat
     7621 catccttgtg ataatcacat tgtttatctt attatttatt gaattagtag aagtgagaat
     7681 accatatatc gatttaatga acgtttccgc aacaaatatg aaatcttatt tatcttggaa
     7741 agttaaccct gcaggcagta ttactttaat gatgagtatt tcagcttttg ttttcttaaa
     7801 aagtggcatt cattttattt tatctatgtt taataaaagc atatcagatg acatgccaat
     7861 gctgacattt gatagtccag taggtatttc agtatattta gtgattcaaa tgttattggg
     7921 ttatttttta tcgagatttt taatcaatac taaacaaaaa tccaaagatt tcttaaagag
     7981 tggcaattat ttttcaggag ttaaacctgg taaggataca gaacgttatt taaattatca
     8041 agcacgacgc gtatgttggt ttggattggc attagttaca gtcattattg gtataccgct
     8101 ttatttcaca ttgtttgtac cgcatttatc tactgaaatt tatttctcag tacaactgat
     8161 tgtattagtt tacatcagta ttaatattgc agaaacaatt cgtacatatt tatattttga
     8221 taaatataag ccatttttaa accagtattg gtaaggaggt aattatgaaa tactttattc
     8281 cagcttggta cgatgaccaa cgatggtggc aagacacgac tgtgccgtat tatcaactac
     8341 aaaataagac ggaatttgac gatatgatta gtttaatggg aatgcacctt gaaaatgact
     8401 tagattatca actgattgtt ctcaatcatg caccaaattt aagaacattt ttacatcgat
     8461 atgacttata tgagacaaag tattcgtctg tgtttgatga aattcaagga ttcagtcacc
     8521 atgcgccaca agcgattaat tatcatcact taaaatggcc ggatgatgtt gagtttgtgt
     8581 acacaccgta tttattaaaa tgtgtgacga gtgaacagac ctatacaaat atttatttta
     8641 gtcaagaagg gtattcaatt tggtttgaag aatttgaaag agatcagtta caacggcgtt
     8701 atatttttga tgacagaggt tatttatcag cgatacgtta ttttgatgat cagggagagg
     8761 cttcttacca agaatattta acgattaatg gagattgtgt acttcatgaa gatttgaaaa
     8821 atggcagagt cactgtttca aaaagatatc aacatcacta tcaacaaaca gaatataaca
     8881 atatggctca actaattgaa gaaaaatttc aagcaatgat tgcacaacaa atacatgaag
     8941 atgatcatgt gattgtggct tcagatgcta ggcacaatcg acaaatagcc aatcatattc
     9001 cagcgaaatc attaagttat tcatttttta aaaatagaaa tgaaactgtg tcagatgagg
     9061 aatatcaatc tatcgtaaag aatgcccatt taattgttga tagtgtgcaa ctagaacgtg
     9121 atttaattag tcatcaagag aagtatcagc gggagaatac aatgattcga atcacaccat
     9181 ttgaaacgag acaatcacct aatataagta gtcaattgat ggaaacattt ataggtgtat
     9241 ggatagatgg tatgagtgac gctgatttgc aacaaatgat gcaacgactt gtggattata
     9301 tcgcacagga agattattac cgtttaattt tattatcgcg ccatcaaaat gacataccga
     9361 tgtggcttcg tgaatgtatt acgtcggtaa atgaggaata ccaagctaaa cagaatgcgg
     9421 atgttaatgt ttcagcatta atgacacctg aagatcaaga tgacatcatt gctgttaaga
     9481 cgatacatgc tgaacatgat gttgtagaag cattgcggac gttgcgactt gtgatagata
     9541 tgtcaaaaga acctgacttg tatttacaaa ttagtgcaat tagcgctggg attccacaaa
     9601 ttaatggtca acaaacagat tacgtctctg attatgacaa tggccgtatt ataaatacag
     9661 ttgatgaatt agatgatgcg ttaaattatt atttatttta tttgaaaaat tggaattatg
     9721 cgtacgccta ttctttaaaa ttaatagatg catatgcttc taagaatatt attaatcagc
     9781 tcgatgagtt aatagaaggt gaaaatgatg ccacgtaaat ttagagtttt gcaaattgga
     9841 ggagacgatt tagaacctat ttttcaacac aaaaaaggtg tgagttggga ttacttcgat
     9901 attggattgt ttgaatttga tagtggttat gtagaggcta ttgaagcgat tgttgaagca
     9961 gaagggcgct ttgattttat ctatattcaa gcaccatact cggagacatt aacgaattta
    10021 ttacaaatga taagcgaacc atacaatacg tatgttgatg aatcattttg gtcagttgaa
    10081 tatgaacaag acgaaaatgt ccaaaaatac gttgttcaac cattacatta ccggaatatt
    10141 gaagaacgta ataataaatt agaggcagtt agcttctcag gacagtatgg agataaggtt
    10201 tctcctaagt tagcacttgt gcatccgaat tttaaaggag atgtcgttta ccaaggtaat
    10261 tcagagctca cattgagtgg agaatttggg aaagaattta aacctatcgc atcttggcaa
    10321 aataatctcg tttacgataa agataaagtc attcaaatat ggccagaatt tgatattgat
    10381 ggtgcggtcg agttgcaata tacatttaga ttgattcaga ctggcgctga tggtgcatta
    10441 attgaacaaa tcgtattgac tgatgatatg ttagacagtc ctttagagat acctgcgaaa
    10501 ccatttgatg cttatataag tgtaactgtt aaggcgcgtg ggaacgggac ggtacattta
    10561 ggacctatac acaaacgatg gtctagatta gacatgggtc aatttttact tggtggtagt
    10621 cgtttcgtag atagtcaacg acaggaattt atttattatt tccaccctgg tgatatgaaa
    10681 ccgccactaa acgtatattt tagtggttat cgaacagcgg aaggtttcga aggatattat
    10741 atgatgaaac gtatgaatgc accgttttta cttatcggcg atcctcgtgt tgaaggtggt
    10801 agtttttata tcggttcatc tgaatatgaa caaggtatta tcaatgttat tgacgagaca
    10861 ttagaaaagc tgaattttaa atcacatgaa ttaatattat ctggtttatc tatgggttca
    10921 tttggtgcct tgtattatgg tgctcagtta aatcctcagg caattattgt tggtaaacca
    10981 cttgttaata ttggaacgat tgctgaacat atgcgattac ttagaccaga agagttcggt
    11041 acagcattag acgtcctggt gtctaatgaa ggtgatacat ctcaagcgtc gatacaggca
    11101 ttaaatcaaa aattttggca aacatttcaa aagaaatcat tgtcacaaac tgtttttgcc
    11161 atagcatata tgcaacatga tgattatgat ccacatgcat ttcaagagct attacctgta
    11221 ttgacagcac accaagcaag agtgatgaat cgcagtattc ctgggcgaca taatgatgat
    11281 tcacctacca ttgcaagttg gtttgttaat ttttacaaca ttattttgga agacaagttt
    11341 gggagagttc aacatgctga aaaacaaaac atttaaagtt acttggaatt atatttcaca
    11401 aactacattt atgtatggct ctaaagtatc attttcaaat ggtgaagtga cttttataaa
    11461 tcctttgatg ccctctggat taccgattca tgagtggttg atgttaaagc aattttcaaa
    11521 atataaaagt gcgccaagtt taccaatttt gcgtagagga cagcattata aattgcattt
    11581 tgattttgat gcaacgcctg ctggttcagt gtattttatc atcatttttt ataataaaaa
    11641 tggcacaaaa ctaagtactg agattgtaaa gtcaaatagt attacgattc aatatcctga
    11701 tgaagcctat gcttataaaa taaaaatgat gaatgcagca tcgacgagtc ttatatttag
    11761 atgtttaaca atcacagaga tgacgcatca atatgattta gagtataaaa gcatgcgggt
    11821 gacgaagata ggcgatgacc aatatggaaa tgatatgatc aatgtcatta ttgcagaacc
    11881 atctgacacc tatccaacta tttcgaatga ttttttgaaa ctgtttggtc atgtttggct
    11941 cgttgaacgt tggatggacg acaatatcga gggaaatatt aagcaactaa agaatgattt
    12001 gcaaagtcaa gataccctaa aggctattaa tttgattagt tatgggtcaa agtcaaatgt
    12061 ttttgcaact tatgtcgctc agcatttggg ttgcaaagtg tatcgtacaa gccatgaaga
    12121 tgatgattta aaaggatggc ttacagaaca tgtcccagga aatcatgaac taaaagatac
    12181 aaatgtcgag gtttatttta aagaagagca agacaaacat ctaaattata tgtcacgttt
    12241 aatgaatcca gtacgatttt tagattactt agatgtatcg aaactgaatg gtggggaagt
    12301 aaatgaaaca taaactagat gttacgataa acgaattaag actaaagtcg attagaaaaa
    12361 tagtgaaacg catcaataca tggagcgatg aagttaaatc atattctgat gatgcgttaa
    12421 aacaaaagac aatagaattt aaagaacgtt tagcatcagg agttgataca ctagatacat
    12481 tgttacctga agcttatgca gtggcacgtg aagcgagctg gagagtatta ggtatgtatc
    12541 ctaaagaagt tcagttaatc ggagcgattg tattgcatga aggtaacatt gcggagatgc
    12601 aaacaggcga aggtaaaaca ttaacggcaa cgatgccttt atatttaaat gctctttcgg
    12661 gaaaaggaac atatttaatt acaacgaatg attatttagc gaagcgagat tttgaagaga
    12721 tgcaaccatt atatgaatgg ttaggtttaa ctgcttcatt aggttttgtt gatattgtgg
    12781 attatgagta tcaaaaaggg gaaaaacgta atatatatga acatgatatt atatatacaa
    12841 ctaatggacg tctcggtttt gattatttaa ttgataattt ggctgatagt gcggaaggga
    12901 aatttttacc acaattaaat tacggtatta ttgatgaagt ggattcaatc attttagatg
    12961 ctgctcaaac accattagtt atttcgggtg caccaagatt acaatcaaat ctatttcata
    13021 ttgtgaaaga gtttgtagat acattgattg aagacgtgca ttttaaaatg aagaagacca
    13081 aaaaagaaat atggctgtta aatcaaggta ttgaagcggc acaatcatac tttaatgttg
    13141 aagatttata tagcgaacaa gcgatggtcc tagtgcgtaa tattaattta gcactgcgcg
    13201 cacaatattt gtttgaatct aatgtcgatt actttgtata taatggtgat attgttttaa
    13261 ttgaccgtat tacaggtcgt atgttaccgg gaactaagtt gcaagctgga cttcaccaag
    13321 ctattgaagc gaaagaaggt atggaggttt caacagataa aagtgttatg gcaacaatta
    13381 ccttccagaa tttatttaaa ctttttgaat cattttcagg tatgacagct acaggaaaat
    13441 taggcgaatc agagttcttt gatttgtatt caaaaatagt cgtacaagta ccaactgata
    13501 aagcgattca acgtatcgat gaaccagata aagtgtttcg ttcagttgat gagaaaaaca
    13561 tcgcgatgat tcatgatata gttgaacttc atgaaacggg gcgaccggtt ttactcataa
    13621 cgagaactgc tgaagcggct gaatactttt cgaaagtatt attccaaatg gatattccta
    13681 ataatttact cattgcgcaa aatgttgcaa aagaagcgca gatgatagct gaagcagggc
    13741 aaattggttc catgactgtt gcgactagta tggcaggtcg aggcacagat attaaacttg
    13801 gtgaaggtgt cgaagcatta ggtggattag ctgttattat tcatgaacat atggaaaata
    13861 gccgtgtaga caggcaatta cgtggtcgtt ctggtagaca aggggatccg ggatcatctt
    13921 gtatatatat ttcactagat gattatttag ttaagcgatg gagcgatagt aatttagcgg
    13981 aaaataatca attatattca ttagatgcac aacgattatc gcaaagtaat ttgtttaatc
    14041 gcaaagttaa gcaaattgta gttaaagcgc agcgtatctc ggaagaacaa ggggttaaag
    14101 ctcgtgaaat ggctaatgaa tttgaaaaaa gcattagtat acagcgagat cttgtatacg
    14161 aggaacgcaa tcgagtttta gaaatagatg atgctgagaa tcaagatttt aaagcgttag
    14221 ctaaagatgt atttgaaatg tttgtaaatg aggaaaaggt gctaacaaaa tcgcgtgttg
    14281 tcgagtatat ttatcaaaat ttaagtttcc aatttaataa agatgtggct tgtgttaatt
    14341 ttaaagataa gcaagcagta gtgacatttt tattagagca atttgaaaag caattagctt
    14401 tgaatcgtaa aaacatgcaa agtgcatatt attataatat tttcgttcaa aaagtctttt
    14461 tgaaagcaat tgattcatgt tggttagaac aagtcgacta tttacaacaa ttaaaagcaa
    14521 gcgttaatca acgacaaaat gggcaacgta acgctatttt tgaataccat agagttgcct
    14581 tagattcatt tgaagttatg acacgaaata tcaaaaaaag aatggtgaaa aatatttgtc
    14641 aaagcatgat tacatttgat aaagagggta tgccagttat acattttcca taaatgaaaa
    14701 ggggtagcgc atgacaatat ataatataaa ctttggtata ggctgggcga gtagcggtgt
    14761 tgaatatgca caagcttacc gtgctaaatt attgagacgt acaaatcaag acactaaatt
    14821 tgtgttttta gattttatac agtcagagaa catacagaca ttaacgcaaa acattgggtt
    14881 taaagatcaa gaagttattt ggttgtatca gtattttagt gatatttctt tagcaccaac
    14941 aacgtacacg ttagatgaca ttgaacgtga gcttggtcat gacattagtt ttagagaaag
    15001 aaatggtaag atagtccgcc tatattttaa tgggaaatca tcatttgtaa catgttatct
    15061 gcaaaatgaa caaaaagata ttgtggatcg tgcagaattt gtaattaata gtatgttagt
    15121 acgtaaagat ttttatagtt atacgcgcat attttctgag tattatgcac cagctgataa
    15181 taaagcgaaa ttatatatgc gacaatttta taatcaagat ggttccatcg cttataacga
    15241 atatattgat ggagacagct ctatatatgt gtttgaagat gcggtgttgt ataacaaaac
    15301 ggaatttatt gcatactttt tacaacgatt gaatttaacc cgagacgata ttgtcatatt
    15361 agaccgcgct tctgatattg gtcaggctgt tttgcagcat aaaggtgata gtaaagttgg
    15421 tgttgtgatt catgctgatc attatagtaa taatatgatg agtgagcagc atatactatg
    15481 gaacaattat tatgaatatc aattttcaaa agctaaatac attgatttct ttattacagc
    15541 aacagatatt caaaatcata tggtttgtcg acaatttgag caatatcaag gttatcgccc
    15601 acgtgtttat actattcctg tgggaagcat tgatgcatta tcatatccaa cattatcaag
    15661 aaagccatat gcgatgatta gtgcatctcg tttagcgaat gagaaacata ttgattggct
    15721 tgtcaaagcg gttatcgtgg ctaaacgaca agtgccagaa ttaacatttg atatttatgg
    15781 agaaggtagc gagaagacgc gactgcgtaa aattattgat acacaccgcg cacaagatta
    15841 tattcgttta ctagggcatg ttaaacttga tgaaatttat aatgattatg agttatttct
    15901 ttctgcatca actagcgaag gttttgggtt aacactaatg gaagcggtag gctcaggact
    15961 aggtatgatt ggattaaatg tgaattatgg taacccaacc tttattcgag atggggaaaa
    16021 tggctacttg gtaccttttg ataccgacga agatagtgtg gatgatgtta tcgcaaaact
    16081 agctcatgcc attgtaatgt attttaacaa tggtccccag acaccacatg acatctctta
    16141 tgaagtggca cagcaattta tgactcaaga tattatttta aaatgggaaa cattagttca
    16201 agaggtgcta catgattaaa ttatttgatt actttaacga tcattcacga aagttatatg
    16261 agtcttttaa agccagtaag ttggaagaag atttaacaat tgtactcaat gacaatggct
    16321 ttttaccgga cgatgttata tcaccgtatc aattttttgc agataatcat aatacagaaa
    16381 atatgaaacc acgatttttt aatcaggtta ctgtaccagc tttttgggaa attaaaggta
    16441 acaacaattc ggctacaatt aatgatatgg gacgtctacg aggtaaaata ttttatcaaa
    16501 gtggagagcg ccctagaatt gttagtcgtg tcgaatggtt tgatgaccaa caacgtgtcc
    16561 gatttgtcga ttattattct aagaatggta ttaaatttgc acaaactgtt tacgatttaa
    16621 atcgtaaagc gatcttgaag aaatatatga cagcagaagg taaagaagtg atttatgaaa
    16681 attttgtaac atctgatgtc atattggatt ggcaagggaa gtcttacttt ttcccttcaa
    16741 agctcgcatt cgcattattt tttatcaagc aacttgaaat tacggaacat catttcgtta
    16801 ttaactcact agccctacca ttttcagtgt tatataattt gccatcaaaa ggtagtgatg
    16861 ttttggtatg gcaagaacac tgtaatggta atgtcccggg aaatatgcaa ttgatgtgta
    16921 aaggtgatat gaaacgacat tgtaatatta ttattccaga taaaaacgaa tatgaaacga
    16981 tgctcaatat agcagacgca aaggttcaat ctcgtatttt acaaggcgga tatctatata
    17041 actatcgtag tcgtaataga tatactaaag aaattgtaat attaactaac tcggatcaac
    17101 ttcgtaacat caaggtattg gttgaaactt taccagattt taagtttcac atcgctgcta
    17161 ttacagagat gtctgataag ctaatgcaac ttgatcaata tgctaatgtg catttatacc
    17221 catctattaa tattgacagg gtaaatgaac tataccagtt atgtgatatt tatcttgata
    17281 tcaatgaggg taatgaaatt ttaaatgcgg tggaacaagc gtttgactat gagttgttga
    17341 ttttaggtta tcgtcaaacc gcacaccatg ctaaagtaac attatcagag cacttatttg
    17401 agcataatga tgagataaca atggaaagta aagaccaatt aatacaaatg ttagaaagtt
    17461 tgaaagatca acaacagttt cgagatgctt tattagcaca aaaagcacat gcacacgaaa
    17521 tctcgcgtga acaattcgag caagttttta aacaggcgct cgaaagctaa aaggaaaaag
    17581 cagtctaaaa taaaacatca tttaatataa aagatttgac gtaatatgat gttagcgaca
    17641 tggacaatat gatattacgt catgcttgtt ttgtttactt aaatataagc taagtaataa
    17701 gtagataatt actaacaaca ataactagat agataagtgt aaatttcttg taaacaggta
    17761 tataatagta tgtaattctt tgaaggaagt aagtgaggaa ggagtatgtt gatggctaaa
    17821 tatcgaggga aaccgtttca attatatgta aagttatcgt gttcgacaat gatggcgaca
    17881 agtatcattt taacgaatat cttgccgtac gatgcccaag ctgcatctga aaaggatact
    17941 gaaattacaa aagagatatt atctaagcaa gatttattag acaaagttga caaggcaatt
    18001 cgtcaaattg agcaattaaa acagttatcg gcttcatcta aagaacatta taaagcacaa
    18061 ctaaatgaag cgaaaacagc atcgcaaata gatgaaatca taaaacgagc taatgagttg
    18121 gatagcaaag acaataaaag ttctcacact gaaatgaacg gtcaaagtga tatagacagt
    18181 aaattagatc aattgcttaa agatttaaat gaggtttctt caaatgttga taggggtcaa
    18241 caaagtggcg aggacgatct taatgcaatg aaaaatgata tgtcacaaac ggctacaaca
    18301 aaacatggag aaaaagatga taaaaatgat gaagcaatgg taaataaggc gttagaagac
    18361 ctagaccatt tgaatcagca aatacacaaa tcgaaagatg catcgaaaga tacatcggaa
    18421 gatccagcag tgtctacaac agataataat catgaagtag ctaaaacgcc aaataatgat
    18481 ggttctggac atgttgtgtt aaataaattc ctttcaaatg aagagaatca aagccatagt
    18541 aatcgactca ctgataaatt acaaggaagc gataaaatta atcatgctat gattgaaaaa
    18601 ttagctaaaa gtaatgcctc aacgcaacat tacacatatc ataaactgaa tacgttacaa
    18661 tctttagatc aacgtattgc aaatacgcaa cttcctaaaa atcaaaaatc agacttaatg
    18721 agcgaagtaa ataagacgaa agagcgtata aaaagtcaac gaaatattat tttggaagaa
    18781 cttgcacgta ctgatgataa aaagtatgct acacaaagca ttttagaaag tatatttaat
    18841 aaagacgagg cagttaaaat tctaaaagat atacgtgttg atggtaaaac agatcaacaa
    18901 attgcagatc aaattactcg tcatattgat caattatctc tgacaacgag tgatgattta
    18961 ttaacgtcat tgattgatca atcacaagat aagtcgctat tgatttctca aattttacaa
    19021 acgaaattag gaaaagctga agcagataaa ttggctaaag attggacgaa taaaggatta
    19081 tcaaatcgcc aaatcgttga ccaattgaag aaacattttg catcaactgg cgacacgtct
    19141 tcagatgata tattaaaagc aattttgaat aatgccaaag ataaaaaaca agcaattgaa
    19201 acgattttag caacacgtat agaaagacaa aaggcaaaat tactggcaga tttaattact
    19261 aaaatagaaa cagatcaaaa taaaattttt aatttagtta aatcggcatt gaatggtaaa
    19321 gcggatgatt tattgaattt acaaaagaga ctcaatcaaa cgaaaaaaga tatagattat
    19381 attttatcac caatagtaaa tcgtccaagt ttactagatc gattgaataa aaatgggaaa
    19441 acgacagatt taaataagtt agcaaattta atgaatcaag gatcagattt attagacagt
    19501 attccagata tacccacacc aaagccagaa aagacgttaa cacttggtaa aggtaatgga
    19561 ttgttaagtg gattattaaa tgctgatggt aatgtatctt tgcctaaagc gggggaaacg
    19621 ataaaagaac attggttgcc gatatctgta attgttggtg caatgggtgt actaatgatt
    19681 tggttatcac gacgcaataa gttgaaaaat aaagcataat tatattgggg gaagagcatc
    19741 tatatatttt tttaagtata taagacgtct tatttcccct taatttattg tgaagtatat
    19801 gcaaaatgca atgaatagat tgtccatcat tttaacgtta taatgaattt aacgacttag
    19861 aactacacaa gtaaaggaga atgaagatgt ctcgaaaaac ggcgctatta gttttggata
    19921 tgcaagaagg tatagcgagt agtgtaccta gaataaaaaa tattattaaa gcgaatcaga
    19981 gagcaattga agcagcaaga caacatcgaa taccagtcat tttcatacgt ttagtgttag
    20041 ataagcattt taatgatgtc tcctcgagta ataaagtgtt ttcaacaatt aaagctcaag
    20101 gatatgcgat tactgaagca gatgcatcta cacgaatact tgaagattta gcaccactag
    20161 aagatgagcc gattatttct aagcgacgct ttagcgcatt tacaggtagt tacttggaag
    20221 tttatttacg tgcaaatgat attaatcatt tagtattaac gggtgtctct acaagtggag
    20281 ctgtattgag cacggcatta gaaagtgtag ataaagacta ttatattact gttttagaag
    20341 atgctgttgg tgatagatca gatgataaac atgactttat tattgaacaa attttatcac
    20401 gctcatgtga cattgaatcc gtagagtcat ggaaaagtag tttatagtta atataacgtc
    20461 aattaaagct cggcagtaat gtttgagaat aagtacattt gctcatattt ataaaatgtg
    20521 tgagatggca attgaaacgg atatgatgag gaacatttga acataaaata atatatttat
    20581 ataaaacgac ccgaggcgtt cgaactgaat gcctcgggtt taattgaata agaaatcaga
    20641 cttatgaaca gaaatatgtt taagtccgaa ctccttgttt atacttataa attttacggg
    20701 tttaatataa tacttattta cctgtaatat atgataattc ttcagcggca gctgcgttga
    20761 tagttctatg agaaatgata cctaatcctt taacattgga ttctgaaata acgatagaac
    20821 catcactgtt aactttttca acaaatgcta catgaccgta atgttgatct gcaccaaatt
    20881 gtccagcctc aaatacaaca gcagcatgac gttttggtgt atgacttact tgataatcac
    20941 ggtattgagc tcgattattc caattatgtg catcacctaa atcacctgag atagatgtac
    21001 caaattgttt catacggtta tatacgtacc aagtacattg gccatgtgga tatggcatac
    21061 tatcagatac ctcacggaaa ggtttgaatt catctgatga atcatcataa tccttgatag
    21121 aacgttcata tttatctaaa tctggcatgc gttcatcgtc aaactgagtt aattgatagt
    21181 gtttaataat actgtttaat ttcttagcat agtttggatc tgtagcatat gttttagata
    21241 agtgtgatgt tgcatcttta taagaatcgg cttccgattt ccatgttggt ttataaattg
    21301 ttcgattgcc atcaatacca tttttaataa ggtcagagta atcttttagt gattctttcg
    21361 tgcttggata ttttcggaat ccagcattaa tactatacaa ttgattacca tcagcttcta
    21421 atgtgttaaa aggaacagaa ttcccttcaa aagcaccttt gataccgaat aaattatggt
    21481 ttggtgactt agctaaagca ctacgacctg agtcagattc taagattgct tgggcaatca
    21541 tgacagacgc ataaatatcg ttatcttgac caatgcgatg tgcatcttta gcaattgatt
    21601 tgacaaattg acgtgtatct tttgagtcaa caacgttaaa ttgtccgcta tcatcattgt
    21661 tagatatact aggatctgtt tcgaataatg atgttgcacg tgtatccttt tgattaacat
    21721 cgttattgaa tgattgagca ggtttagatt tatgtttcaa ttcatcttgt gttggtaact
    21781 gtggattctt tgtattagat ttttcatttt tgtctttttt agattgagat gcataatctt
    21841 tttgtgcttt ctttgcatct tcactgtatt gatccaaaat agagtctaaa gccgaatctg
    21901 acattgattg attatctttc gatgaagatt tttgatttgc tttatcgtca cttgctggtt
    21961 gactatttga ttgattaggt tgtgttggct ttggcgaatt tggttgctta ttagatgtac
    22021 ttggttttgt attgtttgat ttaggtgctt tttgattgtc tgctttatct tgtttagatg
    22081 attgcgtatc agtgtcattt ttgatgctat tgtcactgtt tttattcgaa tcatttgttg
    22141 acttttcgcc attacgaggt tgttcgtaat cagaaatatc cgaatttaaa ttgaataagt
    22201 tttggattaa agttgttaat gagtaattat catcgtattt atttttggtt agcaattggt
    22261 ttatattggt ttgtggtaaa ttcttataaa taaaatcaat gatattgtta gagtctgaag
    22321 tgctgtcgtc tatagtttta aattttttgt cgttattgtc ttggttactt gtattatttt
    22381 tgtctgcttt atcaatatct ttacttgtag tatccttaga agtttcatcg tcattagatt
    22441 tttttgaatc atgagatgtt gtcttagctg tagtatcttt ttgaggtgta tcagcataag
    22501 cggtaggtga aactaaagta ggtaatacga gcgtagttga tagcaaataa attaaaattt
    22561 tatttttagg catatttcgt attctccctt gaaaaatata ataattaagt gtgataataa
    22621 actatgattt gttataattt atcgtatgct gaaaatagtt gataggtatc aatcgactaa
    22681 atatcttcca gtaaattgat tatactaatt cacaacgcaa aaataaatta atttacaaaa
    22741 aatatataaa aaatatgaat aattcctaca taggagtgtg acaatgaaga acgcatttaa
    22801 attatttaaa atggatctga agaaagtagc taagacgcca gctgtgtgga ttatcttagc
    22861 aggcttagct attttgccat cgttctacgc ttggtttaac ttatgggcaa tgtgggatcc
    22921 atatggcaac acgggacaca tcaaggtcgc agtcgttaat gaagataaag gcgacacaat
    22981 cagagggaaa aaagttaatg tcggtaatac gatggttaat acactcaaga aaaataaaag
    23041 ttttgattgg cagtttgtaa gtagagagaa agctgatcat gagataaaaa tgggtaaata
    23101 ttttgcaggt atttacatcc catctaagtt tacacatgaa attacaggga cactacgtaa
    23161 gcagcctcaa aaagcagatg tagaatttaa ggtgaatcag aagattaacg ctgttgcgtc
    23221 taagctaaca gatactggtt cgtcagttgt cgttgaaaaa gcgaatgaac aatttaataa
    23281 aacagtaact cgagcattat tagaagaagc taacaaagca ggtttaacta ttgaagaaaa
    23341 tgtgccgaca attaacaaga taaaaaatgc ggtatattca gcagataaag ctttacctaa
    23401 gattaatgac tttgcgaata aaattgtata tttgaataac caccaagcgg atttagataa
    23461 atatgccaat gattttagaa aactaggaaa ttataaaggt gatattttag atgctcagaa
    23521 aaaattaaac gaagtcaatg gtgctattcc gcaacttaat gaaaaggcta agttgatatt
    23581 agctttaaat aattatatgc cgaaaattga aaaagcgtta aattttgcag ctgatgacgt
    23641 gccagcgcag ttccctaaaa ttaatcaagg acttaacatt gcgagtcaag gtattgatca
    23701 agctaatgga cagttaaatg atgccaaagg cttcgtcaca caagttagaa gtagagtcgg
    23761 tgattatcaa gatgcaattc gacgcgcgca agatttaaat cgaagaaacc agcaacagat
    23821 tcctcaaaat agcgcggcga acaacgaaac atcaaatagt gcacctgcag ctggtaatgg
    23881 tgtagcatca acgccaccaa gtgcaccaag tggcgatact gcaccaaata ataatgttac
    23941 gcaaaatacc gcaccaaata gtaataatgc gcctgtatcg actacaccac aaagtacaag
    24001 cgggaaaaaa gatggtcaaa gttttgtaga tataacaaca acacaagtca gcacagctaa
    24061 cgagaacaca caaaacatta cagataaaga tgttaaatca atggaagcgg cattaacggg
    24121 ctctttatta tcattatcaa ataatttaga tacccaagcg aaagccgcac aaaaagatag
    24181 tcaggcatta cgtaatattt cgtatgggat tttagcatcg gacaagcctt ctgattttag
    24241 agagtcttta gataatgtta agtccggttt agaatacaca acgcaatata atcaacaatt
    24301 tatcgataca ttaaaagaga ttgagaagaa tgaaaatgtt gatttatcaa aagaaattga
    24361 taaggtaaaa gcagctaata atcgaattaa tgaatcatta aggttagtta atcaattaag
    24421 caatgcatta aagaatggta gttcaggaac tgctgaagct actaaattac tagatcaact
    24481 ttcaaaacta gattcatcat tatcatcatt tagagattat gttaaaaaag atcttaacag
    24541 ctctttagta tcaatatcac aacgtattat ggatgaattg aacaaagggc aaactgcatt
    24601 atccaatgtt cagtctaaat taaatacaat tgatcaagtc attaacagtg gacaagctat
    24661 tttaaaaaat ggtaaaacac gtattgatcg cttacaaaca gtattaccaa gtattgaaca
    24721 acaatacatt agtgctgtta aaaatgctca agcaaacttc tcgaaagtga aaagtgatgt
    24781 agctaaagct gctaactttg tgcgcaatga cttaccacag ttagaacagc gattaactaa
    24841 tgcgacagca agtgtgaata aaaatttacc aacgttatta aatggttatg atcaagcggt
    24901 aggattacta aataaaaatc agccacaagc gaaaaaggct ttatcagatt tagctgattt
    24961 ttctcaaaat aaattgcctg atgttgaaaa agatttgaaa aaagcgaata aaattttcaa
    25021 gaaattagac aaagatgatg cagtcgacaa attaatcgac acacttaaga atgatttgaa
    25081 aaagcaagcg ggtattattg caaatcctat taataagaag actgttgatg ttttcccagt
    25141 taaggattat ggttcaggta tgacaccatt ctatactgca ctgtcagtat gggtaggtgc
    25201 actcttgatg gtaagtttat taacggttga taataaacat aagagtctag agtcagtctt
    25261 aacgacaaga caagtgttct taggtaaggc aggattcttt ataatgcttg gtatgttgca
    25321 agcactcatt gtatcggttg gagatttgtt aatcctaaaa gcaggagttg agtcacctgt
    25381 attatttgta cttataacga ttttctgttc gattattttc aactcaatcg tatatacgtg
    25441 cgtatcatta cttggtaacc caggtaaagc cattgcaatc gtattgcttg tattacaaat
    25501 tgcaggtggt gggggaacat tcccaattca aactacgcca caatttttcc aaaacatttc
    25561 gccatactta ccatttacgt atgcaattga ttcattacgt gaaacagtag gcggtattgt
    25621 tccggaaatc ctaattacaa aattaattat attaacgtta tttggtatag gattcttcgt
    25681 tgtaggttta attttaaaac ctgtaacaga tccattgatg aagcgcgtat ctgaaaaagt
    25741 tgaccaaagt aacgttacag aataaaaatt aaatccacac attagggtta tagctcctta
    25801 atgtgtggat ttttatgttt ttagacagaa gagatagtaa tttctgtctt ttatgggacg
    25861 gttgttatca ttgctattat ccaggatgac ttactatagc actaatatta ccgacaaagt
    25921 gaatatcctc gtcttccgta gttaaaataa agctagaacc tttttggatg tcatagtgct
    25981 tatcgtttac tgttaaagta ccagtaccat cgataattgt aactaagcaa taagcatgtg
    26041 gtttattgaa ttttaaatct ccatgaatat cccatttata tactgcaaaa tattgattat
    26101 ctacaaattg agttacagtg tgtgtgtcga tgtgagttgt tataggagta gtatttggtt
    26161 catgattgcc taattcaatc acatctttac tttgctctaa gtgcaaatca cgcaattgac
    26221 cattttgatc tcgtctatca tagtcataaa tacggtatgt cgtatcggag gattgttgtg
    26281 tctctaaaat taaaataccc gaaccaatgg catggacagt gccagcagga acataataaa
    26341 agtcaccggg cttaacaggt atacgtttga aaagactgtc aaattcatga ttatcaatca
    26401 tgtctattaa cgtctgttta ttatgtgcat gtacgccata tataatttca gcacctgggg
    26461 ctgcatctaa aatataccaa cattctgttt tacctagttc gccttcgtgt tttaaagcgt
    26521 agtcatcatc tgggtgaact tgaacagata atttatcatt ggcatctaat actttagtta
    26581 gcagagggaa actatctcgt gaatcattat cgaataattc acgatgttgt gaccaaagtt
    26641 gatctagggt catatccttg tatggaccat tgataattgt attaggacca tttggatgtg
    26701 cagaaattgc ccagcattca ccagttgttt cattagggat atcatagtta aatgctttta
    26761 atgcatgacc gccccaaatt ctgtctttaa aaacgggttg taaaaataat gccatagtta
    26821 aaactcctct atattttcat taataagtta taaatttctg tagtactgtt tgcattaatt
    26881 agtgattggc gtgtctcatc attcattaac gctttagata agcgctgaag tatttttaaa
    26941 tgtgtatcct gactgttgtt tggtacggca attaagaata tcaattgagg tagactacca
    27001 tctagactgt cccatttaac accatgatta tttttcataa cagctacaat cggttgtttt
    27061 acaacatcag actttgcatg tggaatggcc acgttcatgc caatagctgt cgtagacccc
    27121 atttcacgtt ctagtattgc attttttaaa tgcgatgtgt gctctacata acggcaaatt
    27181 ttaagtttat gaatcaacat atcaattgct tcgtttcgag acatgtcgtg atcagtaatt
    27241 atcatagttt gttgatcaaa aacatgagaa ggtttattga gatgtgaatg tttcgcggtg
    27301 ttatctacat tgtcaacctc tgtatcatgt tgtgtaatat ctgtatcatg aagttgcgtg
    27361 tgttgcgctg gtgcatctac tgctataact ggtgtattgc gttttaataa tagtacagta
    27421 gtcattgtga caagactacc tactatcact gcaaagataa accataatac atgatcaata
    27481 ccacctaata cagccacgat tggacctcca tgtgcgactc tatcgccgac accaccaatg
    27541 gctgcaatga ctgatgcaat cattgcacca atgatgtttg caggtataat gcgcaatgga
    27601 tcttgggctg cgaaaggaat agcaccttca gtaataccaa atagtcccat agtgaaggaa
    27661 gccttaccca tttctctttc ggaatgattg aatttatact tttgaacaaa cgttgctaaa
    27721 cctaaaccga ttggtggtgt acatacagca actgcgacca tacccataac ggcgtaatta
    27781 ccttcagcaa taagtgctga gccaaataaa aatgctacct tgtttactgg accgcccata
    27841 tcgaaggcaa tcatcgcacc tataatcatc gcaagtataa taatattagc accttgcata
    27901 ctttttaacc aggttgttaa tgcctcaaaa atattagaaa ttggtgcacc gattaaaaat
    27961 ataaatatca atcctacaac gaccgatgaa ataatgggaa taataatgat aggcataatt
    28021 ggtgccattg cttttggaac tttaatatct ttaatccact ttgcgatata acctgctaag
    28081 aaaccagcaa caataccacc taaaaatcct gcgcctgcat cactgccata aaaactaccg
    28141 tcagcagcga tagcgccgcc aatcatacca ggaacaagac cgggcttgtc agcgatacta
    28201 acagcgatat atccagctag tattggaacc ataaatttaa aggctaaact accaatgttt
    28261 tcaatggatt tccaaaatga atcatctggg atgactaatc cttttgatgt cgtttcaccg
    28321 cctagagtca gcgcgatggc gataaggagt ccaccaacta cgataaaagg aaccataaac
    28381 gatacaccgt tcattaaatg ttgatacacc atttgaatac cacttttaga cttaccgcga
    28441 tctttcgaat gataatttgt ttcagattga taaataggcg catcttgatt aatgatacgt
    28501 tgaattagac ctctcggatt atgaatccct tcgcgaacat tttcattaat caaccgttta
    28561 ccaacaaatc gggacagatc aacttgttta tcagctgcaa ttatgacacc gtcagcttct
    28621 tcgatgtctt gcgtagttaa aacattttca gcaccaacac cgccctgtgt ctctacttta
    28681 atatccacac ccatttcttt tgctacctgc tcaagctttt cttgagccat atatgtatgt
    28741 gcaatgccat ttgggcatga ggtaatagct acaattttca taaaatcatc tccttttcta
    28801 tattgtaagc gtattctcga tactaaaaaa aagaataatt accgttacta gtggcaatta
    28861 ttcttgtaag tattcaaata actgttgctt taaactatga tcatctaaac tacataaatg
    28921 gttcactgaa tcatcatcca agttagcaat taattgcatc atttgttttg taaaagcttt
    28981 gtctttatgc gaaatcgcta agaaaaagac aagtttgaca tcgtgttgtc gccaaggaaa
    29041 aacatctttt gtgcgaaaaa taagcacatg tgattgtaaa actttttcag gatctccatg
    29101 aggaatcgcc ataaaattac ctatgtatgt agaagatgat ttctcacgct ctaaagctga
    29161 ttcgatatat ccttctacaa tcgcatgatg tgcttgtaat attttttgag cttcttcaaa
    29221 aatttgcaca gtatgccgtg atttttgttc agtatttacg acaaggaaat tgacagtgtc
    29281 catatgatga tgtgcttgaa ccggattttg cttttgcttc acaacgtgtc tgattttgtg
    29341 acgatcatct tcagaaaata atggtgcaac cttgatagtc gtcaggtgct taggaagtat
    29401 gtttagcgtt tgtttaggaa tatcatgggt cgttattaat aaatctacat tgtcaaagtg
    29461 atagtgtgtt atattttcta gtttaatcgt atttatcact gacaactctt cggataagtt
    29521 atttatttta gtttctaaaa aattcgacac acctagacca taataacaag caatgactac
    29581 atttaattgt gttttggtac gacgctcgat ggcagcttga aaatgaattg ttaaaaatgc
    29641 aatttcatct tcgctcatct ctatatcagt atcaattgct aatttatcaa tcgcttcaaa
    29701 aagtgtgtta aacacaaagg gatagagttt tttaatctct ataactaaag gattgtttaa
    29761 ataaatgttt tgagtgatac gtaaatatgc tttactaaaa tgattatata aattttgttg
    29821 taaaatcgaa tcttcattga aaggtacatg aatacgttgc tgcatcaatt cgattaagcg
    29881 atcaatataa ctttgtataa atatacgttc tatgccaata tcgagtttat taaaatgata
    29941 agcaataaag aatgaaaaca tattgattac tttttcgttc aagtcataac ctaatctttc
    30001 gttgatttgc ttaatgcaag attgagatat caattttgaa tcgtggttta acgtgtctag
    30061 ctcacttaaa gattgcttac gtttaatgat taaaatgaga tgaattaaaa gctgttgtat
    30121 ttgaatatca gttgtttcaa tactatgttg ttgaagtgtc tcttgtataa tatgcgaaat
    30181 catcctttgg tgtgaatcag gtaattcatt taaaattagg tcttcaacat gtacatgccc
    30241 tgatgataat tgatttaaat ggatgatggc attagtgata tcattatctg ttccatcgac
    30301 cacaatacct tgttttttct tgattgttaa agcgatatta aatttttgac accattgttg
    30361 aattcgaacg aaatagtcgt tgagcacagt tttagataat agataatctt gctctagttg
    30421 ttgcaatgtc tgcgatttat tgtgcattag cagttgatac gccatagata ataatagttt
    30481 ttctttatat gattgaatat gcgtgtaacg ttcagttatc gtttctaagg tatactgaga
    30541 tgtatttaat tgatatcctt tagatttaat acttacgatg atatcatcca taaaattgct
    30601 attgataaca tggatatcat tgcgaactgt gcggtttgaa acattgacat gtttagcaat
    30661 ttcattagaa ctaatgtgct ttgatggatt tttaataaaa aactggagta gttttaagtg
    30721 tctatcaagc atttaacatg tacctccttt ctaaattttt cgtgtaagcg tttttaaggg
    30781 tgtattaata ttattaaaca tgagagctta tacatacgtc aatgacatta aagcgaactt
    30841 ttatatgatt ttaacagagt gcgaattatg caaataaaga acagcagtaa gatatttcaa
    30901 atagaaaaat atctcactgc tgtttttctg aatttatgca tctggtactt gtggacgtat
    30961 caggcaaatg attaattttt aggtgattgt gcttgaggtg tttgtttaga aggcgtattg
    31021 gtattatttg atttattagc aggtggtgtg ttctgttgtt gattattttg gttgtttgtt
    31081 gacgattgac tgccaccacc gtgagtatta tttttatttg aatttgagtc ttttggctga
    31141 gctggttgcg tattcgatgg tactgttgaa tggttgttag gaacatttga cggtgaagat
    31201 ggttgattgt cattagtaac gttattgcca ttattttgat ttttatctgt tttgtctgca
    31261 ctatcatctt gttgatcatt ttcttcggtt tctgtctttt tatgcgtaga tttattttct
    31321 tgttcttggt tattacttga tgatgtagaa tgatttattg cagcataaat tgcgaaccca
    31381 agtgctccga ttaatacgat aattacgatg gtaataagaa gttttttcaa atataacgcc
    31441 tccatgttaa tgaaaagtag ttgatacgtg aagctaaata atttatttca atattgccat
    31501 aatcatttta aaaaataaat attagaaaag taggcgatat ta
//
LOCUS       DAMQYT010000012        29327 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMQYT010000012 DAMQYT010000000
VERSION     DAMQYT010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 29327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29327
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.012"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(74..1954)
                     /gene="vraE"
                     /locus_tag="PGA33_000457"
     CDS             complement(74..1954)
                     /gene="vraE"
                     /locus_tag="PGA33_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HDG6472296.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFKSALFLKRRSILSMMKDSIKTDATTAKVTTAEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNAYETITVDNVNDQVITLENGSDSGRTNSILSANNKLTGN
                     NAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKK
                     TI"
     gene            complement(1944..2702)
                     /gene="vraD"
                     /locus_tag="PGA33_000458"
     CDS             complement(1944..2702)
                     /gene="vraD"
                     /locus_tag="PGA33_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HDG6472297.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            2985..3497
                     /locus_tag="PGA33_000459"
     CDS             2985..3497
                     /locus_tag="PGA33_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HDG6472298.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIVYRRKAKALVGGNQNWILFMGIVDILFGLLVIFNVGASSAFFIYMFAFW
                     FIFSSISGLFTFSGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            complement(3631..4425)
                     /locus_tag="PGA33_000460"
     CDS             complement(3631..4425)
                     /locus_tag="PGA33_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HDG6472299.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPLHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTHPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKFDVTKDNYKHFLEKYFGLNVTINRIEKQ"
     gene            complement(4668..5684)
                     /locus_tag="PGA33_000461"
     CDS             complement(4668..5684)
                     /locus_tag="PGA33_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HDG6472300.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSR"
     gene            complement(5849..6424)
                     /locus_tag="PGA33_000462"
     CDS             complement(5849..6424)
                     /locus_tag="PGA33_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDG6472301.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVQSSLPFKLTGDVSCNHAPKRQQMIDFIVVTAALDIELLRS
                     LVTTEFIWSVPGRFDIYGPQILIQELSNHYNQVASLNIHSSITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            6649..7557
                     /gene="rarD"
                     /locus_tag="PGA33_000463"
     CDS             6649..7557
                     /gene="rarD"
                     /locus_tag="PGA33_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDG6472302.1"
                     /translation="MNSEYKKGIFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            complement(7970..9388)
                     /locus_tag="PGA33_000464"
     CDS             complement(7970..9388)
                     /locus_tag="PGA33_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDG6472303.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVVALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(9626..10090)
                     /gene="bstA"
                     /locus_tag="PGA33_000465"
     CDS             complement(9626..10090)
                     /gene="bstA"
                     /locus_tag="PGA33_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HDG6472304.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDAWKVEKVLDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIERILEGLQTLPER
                     ARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            10257..10595
                     /locus_tag="PGA33_000466"
     CDS             10257..10595
                     /locus_tag="PGA33_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDG6472305.1"
                     /translation="MSVSDQIMKGLLDGAILGLIAQGETYGYEIMTKLKNQEFPEISE
                     GSIYPVLMRLNKKGYVDTTIKKSSNGGPRRKYYTITDSGLKELKVFKSKWQVLNKGMI
                     NLFGGENDVK"
     gene            10585..11352
                     /locus_tag="PGA33_000467"
     CDS             10585..11352
                     /locus_tag="PGA33_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472306.1"
                     /translation="MLSKQSQDFLEKLRVELLFRGKTEAEVEELLEELEDHLTMAEKN
                     NEDTSSIINTPIKSMADQLSPEISLTTGLYKYITLYFAFILAVIIIPRFLDLGTFDVT
                     IAFLLYIASIVILGVVIGMLILRNTLVRFGDQKITYVINVTYGIIVFLWMILGGLIIK
                     KYPIYSFFELSDKQSFIVGLVLLIALIGTCILIKQKIYALIVLVISLPSLIARIAAIF
                     SKEPDTFNLVSVSILIILNIAFIIYTFVNFRKLKKED"
     gene            complement(11471..12109)
                     /gene="pcp"
                     /locus_tag="PGA33_000468"
     CDS             complement(11471..12109)
                     /gene="pcp"
                     /locus_tag="PGA33_000468"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDG6472307.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHTLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVIGKPDTPSMPLEKIVAGLTAAIEAISNDEDLHLALGTTE"
     gene            complement(12261..13217)
                     /locus_tag="PGA33_000469"
     CDS             complement(12261..13217)
                     /locus_tag="PGA33_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDG6472308.1"
                     /translation="MNYQVLLYYKYMTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDD
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            13461..14432
                     /locus_tag="PGA33_000470"
     CDS             13461..14432
                     /locus_tag="PGA33_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDG6472309.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            complement(14581..15096)
                     /locus_tag="PGA33_000471"
     CDS             complement(14581..15096)
                     /locus_tag="PGA33_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HDG6472310.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSVSESKVVGDLIIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGIIVTGTINREKYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            15476..16324
                     /locus_tag="PGA33_000472"
     CDS             15476..16324
                     /locus_tag="PGA33_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HDG6472311.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREVTKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            16353..16907
                     /locus_tag="PGA33_000473"
     CDS             16353..16907
                     /locus_tag="PGA33_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HDG6472312.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMVYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            16960..18672
                     /locus_tag="PGA33_000474"
     CDS             16960..18672
                     /locus_tag="PGA33_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6472313.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLKDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLVLNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            18669..19502
                     /locus_tag="PGA33_000475"
     CDS             18669..19502
                     /locus_tag="PGA33_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDG6472314.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISASSILLVKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELV
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     H"
     gene            19549..20046
                     /locus_tag="PGA33_000476"
     CDS             19549..20046
                     /locus_tag="PGA33_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6472315.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWRNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLKFKRDDTMDDIINQGD
                     IVFYK"
     gene            20164..21288
                     /locus_tag="PGA33_000477"
     CDS             20164..21288
                     /locus_tag="PGA33_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDG6472316.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRRYFKGNRDYEE"
     gene            21593..22411
                     /locus_tag="PGA33_000478"
     CDS             21593..22411
                     /locus_tag="PGA33_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDG6472317.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHK
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            22429..23043
                     /locus_tag="PGA33_000479"
     CDS             22429..23043
                     /locus_tag="PGA33_000479"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDG6472318.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELAGVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            23036..24286
                     /locus_tag="PGA33_000480"
     CDS             23036..24286
                     /locus_tag="PGA33_000480"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDG6472319.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            24302..25315
                     /locus_tag="PGA33_000481"
     CDS             24302..25315
                     /locus_tag="PGA33_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDG6472320.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLN
                     VFTLNIATYIFRHREETRQFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            25284..25862
                     /gene="hisB"
                     /locus_tag="PGA33_000482"
     CDS             25284..25862
                     /gene="hisB"
                     /locus_tag="PGA33_000482"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDG6472321.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            25859..26437
                     /gene="hisH"
                     /locus_tag="PGA33_000483"
     CDS             25859..26437
                     /gene="hisH"
                     /locus_tag="PGA33_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDG6472322.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            26430..27134
                     /locus_tag="PGA33_000484"
     CDS             26430..27134
                     /locus_tag="PGA33_000484"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDG6472323.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRRLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            27131..27889
                     /gene="hisF"
                     /locus_tag="PGA33_000485"
     CDS             27131..27889
                     /gene="hisF"
                     /locus_tag="PGA33_000485"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDG6472324.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            27886..28518
                     /locus_tag="PGA33_000486"
     CDS             27886..28518
                     /locus_tag="PGA33_000486"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDG6472325.1"
                     /translation="MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            28711..28890
                     /locus_tag="PGA33_000487"
     CDS             28711..28890
                     /locus_tag="PGA33_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472326.1"
                     /translation="MKSKLSNSGIDVTVKDVEKYMNRYNEVMKGKNGEKAKELCLSLL
                     PINIIVVFTFFVFIL"
BASE COUNT        10152 a         4443 c         5309 g         9423 t
ORIGIN      
        1 ttaaatcaat taatagttat actgcattgc ttaaaatcaa aatatctaat gaagcatctt
       61 ttaatcgtaa gtgttaaatg gttttcttaa tcaatttgtt tgaatgtaca aaagcaatta
      121 aagcaaaagt aatgtaaatg agggtgtata caacaattac tacaataacc ggcatgaagg
      181 aaatattacc catcaatttc ataaatgcta ttgcggcgaa tactgcatgt aatatagcaa
      241 tcaataatgg taaaccgaag ttgaaagtaa tttttaataa taatccctta agcatatctg
      301 tatgcgtaaa gcctatgcgt tttaatattc tgaagttact tagttcatcc tcagtttcat
      361 ccatttgttt aatataaata atacatccag ctgctactaa aaatgctaat cctaaaaatg
      421 atgtaacaaa tattagaata ccgttagtag catcgacctc ttttttcatt tcatcatacg
      481 tgatgacttt gtctccaaac tgttttgcaa ttgcttgagc tttttccttt tgtgatgttt
      541 gtttaatatc atatccataa aaagtatgaa cgttattttg tgttttcaac tgctgatact
      601 tttcaggact tacttcgatg acaggtgagt tgaagcttag atttaaagga taaaccttac
      661 ctttgtcttc ttgtgtaaca cggaaagttt cattcttagt accttttact actaaatctt
      721 tgtttaaatg gatattaata atgttaggca gcgattttgt atttgtaatg atggcattgt
      781 tgcctgttaa cttgttattt gcacttaaaa tagaattcgt gcgacctgaa tcactaccat
      841 tttccaaagt aataacctga tcattaacat tatctacagt aatagtttcg taagcatttt
      901 tagaaaatgt aatttgttgt tggcttagtt tagtttcaaa ttgtttagca tcttgagtag
      961 cgaccacgtt aaattcattt ggtgccatag atgtaagggt ttgatctgta tttgatttag
     1021 ataatgccgc aaaacacaat acagttactg taactgcaga aataattgca atgatagtta
     1081 aagacatggc atttttcttc attctgtaca taatagacga tgtgaataca acatcggtaa
     1141 tagatacgcg tccatttttt gattttttca atgttttaaa aataagtgac acggaacttc
     1201 tgaagaataa ataggcgcct acaaccgtta aaaataaaat gataaacggt gatgtcatag
     1261 ccatagttag tgctttgaac gtaccaaaca tttctgtcgc catataatag cctagtgcaa
     1321 tcatagcaat acctaatacg cctgaaataa cctctgcagt cgttacttta gcagttgtgg
     1381 catcagtttt aattgaatct ttcatcattg ataagatact acgtcttttt agaaataaag
     1441 cacttttaaa taaaatcagt acatacgcaa taattagcat gaaaatagtt aaaacaaggg
     1501 ccataggttc gaaatgtatc gataagttaa tcgataacga catcaattta gatactatgg
     1561 aaagcaataa ttgtgcacct gcaatgccac ataatacacc gacaacacct gtgattaaaa
     1621 atacgatcat ttgttcaagt gctaacattt tcaaaatgtt ttgtcgtgtt aaaccaatca
     1681 actgaaatag cgcaaattca cgtgtacggc gttttacgaa taaatgattg gcatacatta
     1741 aaaagatgac aataatgata aataaaaata ttgatccgac taaagcacct ttcttaatga
     1801 tggccatcga gtcgtcatta tttacacctt tagtaaactg taaggttgta aaactgaaat
     1861 ataagacgat gctaaaaaat aatgaaaata aatacattgc ataatgtttt aagttttgtc
     1921 gtaagttttt gaaaacgata tggttaaatg tcatttgaga caccacctaa tactgattga
     1981 agatgtacaa tgtcttcata aaaggcctgt ttagaacgtc cttcctgata aagttgtgta
     2041 tgaatttggc catcttttag catgatgaca cgttctgcaa aacttgctgc aaccggatca
     2101 tgtgtaacca tgacaatagt tgtatcaaac gatttattca tttcttccaa acgttgtaat
     2161 aggtcatttg cacttttcga gtcgagtgcg cctgttggct catctgcaaa tatgatttgt
     2221 ggtttgtgaa caaatgctct cgctgctgca gttctttgtt gttgaccacc agataattcg
     2281 ctagggtatt tatttcctag gtcataaata cctaatgctg tcgtgatcgc tttataattt
     2341 tcttccattg ttgccttcga cattttttga acagataaag gtaacataat gttttcttta
     2401 acggttaatg tcggcagaat actgtaatct tggaagatga aacctaatga ttctttgcgg
     2461 aatttggcaa gtgctttttg attaagttta ttaagctctt gtccgttagc aatcacgcta
     2521 ccgctagaaa tttggtcaat tgaacttagt acatttaata aggttgtctt acctgatcca
     2581 gaaggcccca taatcgcaac gaattcgcct ttttgtatgt caaagttaat atctttaagt
     2641 gcttgaaatg tgtgcttttt accgtatgtt tttgaaacat gttgcactga taatatcgtc
     2701 atagtctcac tccttttgta tttaatttca ttttaaataa tgtttggagt agtagccttt
     2761 atctaaactt acaattcaat gaatgaacct tacagagttg aaagctatcg ctacttagta
     2821 gatttttgag tgaggataca gattcatcgt acatattaga caaaagcaat ggtgctttct
     2881 aagtgatgat gtttgtgtaa attgagaaaa gggaatttaa ttattgtata ataaattttt
     2941 tgtaaaaatt aaaagagggt tttatttgaa aggaattgat tgttatggaa aaaggaaatc
     3001 aaggtattaa atggtctagt ttaataatgg gtgtattatt attaatgttg gcagtcgtta
     3061 tttttacatt tccaattgaa aatttttatg ctattacctg gttgattgga ctgtttgtat
     3121 taattaacgg tgtgattcaa atcgtttacc gtagaaaagc aaaagcttta gtaggtggta
     3181 accaaaattg gattctgttt atggggattg tagatattct atttggtcta ttagttattt
     3241 ttaatgttgg cgcaagttca gcattcttta tttatatgtt tgctttttgg tttattttta
     3301 gttctatctc tggattattt acgttttcgg gtagtggtag cttaaaacta atttcagtga
     3361 tttttaattt attaggtatt gttttcggtg tcattttatt atttaatcca ttaatgggta
     3421 tcgtctttat ttcgacgatg attgctattg catttgtatt cgtaggtgtc atttatgttg
     3481 tagatgcact tgcttaagta aaatgaagcg gttcaaaaga agggtgtgac atgaagtttg
     3541 tgtcatatcc tttttgttgt gtttatgaag cataaaaaag gggcgctacc tacaataagt
     3601 aagatacgcc catattttta tattttacta ttattgtttt tcaatacgat taatagttac
     3661 atttagtcca aaatattttt ctaaaaaatg tttatagtta tctttagtga catcaaattt
     3721 ttctgagcta ccattccttg ttaaagttaa atgattttca gacattgtag cacggccaaa
     3781 tgattgtggc attgtaatta ataaatgctg tacaaatatt gaatctggat gcgtttgatt
     3841 atattcgatg ttgctattaa aatcttctat ttgtttaggt ttgaattcag cttcatattt
     3901 tgtatgccaa tgatcatttt cgaatttttg aacatagaaa atatccttgt cttcgttgtt
     3961 aaaaatagcg cggaatgtac cactgatatc agtaattggt tgtgtatgct cagatgaagt
     4021 aataggaatg gcatgtagag gtaagtctcc aaagccaaca tcagttacat agaatacatc
     4081 atttatagaa acaacaagtg aagcatgtga accgttcaga ctacgaccgc caccgggtgt
     4141 gtgaatagta gctgacatta attcaggatt aaatcctttt tgttgtaaat aggctttgaa
     4201 aaatgtattt aattcataac aaaaaccacc acgtttatca tgaacaattt tattaaaaag
     4261 tgcatcgata tttatagata tcggcttact attttgaaca tcaatatttt caaaaggtac
     4321 agttaacata aaacgtgttg cataataatt taatgcttca atactcggtc gattataacg
     4381 agatgaatca atttgtaaat aattctctaa cttcgcaata ttcataagca tagcgcctcc
     4441 tgtattaaag attataatta aattttaaac agaaatactg aaattttaaa ttcgaaagca
     4501 ttgaattttg gataaataca ttttaaatag aaaaatacgc tctcaaaatg aagtcatctc
     4561 taaaagaaac gatttaaaga tgactactga gagcgtagca taatggaaga agtgtgcagg
     4621 gtgtctaaaa atgcaacaat acaaaggtag ttgcaagaca agttgcctta tctagaccat
     4681 ttgtgttcta tgcgaccaaa cttccaaatt aaacttgaaa taagccaagt aattaaaaat
     4741 aatgcaacta aaatatagcc taaataatca aattcgatcg aaccaatgaa tgcccaaaac
     4801 gcaccatgta aatctaactt atcagcaaga atttgtagca attcaatcat cccaatcact
     4861 aatgctgcca tgactgatat cgcagtaatc gttatattgt aatagatttt gcgaatagga
     4921 ttgaagaatg cccaattata ggcatacttc attacaacac catctaatgt atccaataaa
     4981 ctcatacctg atgcgaataa aattggtaaa gataagattc cgataaatga aatggcttgt
     5041 tgtgatgcgc ctgaagaaag agcgagtaac gcaatttcac tagctgtatc aaaaccaagt
     5101 ccaaataaaa agccaagtgg caatacgtgc caactacgcg tgattaattt gaaataaggt
     5161 cctacaaatc gagaaaccaa tcctctagat tcaagtaatg catcgacttc agcttcttca
     5221 atgtgttcac gacgtaattt agcgaacaag ttaattaaag agattaaaat aattagattc
     5281 aacacaccga taagcactaa aaagaaacct gaaactagtg taccaatcgt tccaccaata
     5341 tcttggaaat gcggtaattc atctttagcc cattttacag atacccctaa aaaaacagcc
     5401 attaaaaata cgacagatga atgtccaatt gaaaaataga aacccacacc agatggatct
     5461 ttgcgttgct gtaataattt gcgaaccgta ttatctattg cagcaatgtg atctgcatca
     5521 aatgcatgac gcaaacctaa tgtatatgca agaatcccca taccaaataa gatatgatgg
     5581 tcttttccag caatccataa aaaactaaac ccaataacgt gtaacaaaat gacaatagct
     5641 atgtatggta accagcttaa gcgctcattt ttaaaaacag tcaaaatata tccttctttc
     5701 ataacatgat atttaatcat actgtatgtt caatgggcac tctagtaata agtgttcata
     5761 taacaaaaat gttatgccaa attatttgtt atataaaaat atacatgtaa ccacaaaaga
     5821 ttttttgcga tatatataat ttgataaatt aaccaacaac aatgtaagat gtcactttgc
     5881 ttaacttggc atccttttta tgattttcaa attcaaaaaa atgagcaaaa tgaatctctt
     5941 taccagtttt taatatttca ataccatgca tggaacctaa gcacccatgt gtgatgctgg
     6001 aatggatatt gagactagca acctgattgt aatgattaga tagttcttga attaatattt
     6061 gaggtccgta tatgtcaaag cggccaggga cagaccaaat aaattctgtt gtaaccagtg
     6121 aacgtaataa ttcaatatct aatgctgctg taacaactat aaaatctatc atttgttgac
     6181 gtttaggcgc atgattgcat gacacatctc ctgttaactt aaaaggtaat gatgactgaa
     6241 cttccgtttt aaaatgtagt tggtgctgaa ataaagcttg ttccaatatt gatatagctt
     6301 taggaccaac gccatgcatc tccattagag atgacttcgt atattgtgat actgcttcta
     6361 atgtgtatat accttgtgaa tttagcgcgc gtgttgcagg ctttccaatt ttaggtaaag
     6421 tcatttgtat ccatccttta tgttgtatgg tagtctttct ggcaatatac cctgatttgt
     6481 acagaattat ttataaaatt aatattgttt agtggttggt gaataaatgt gggaaatata
     6541 aacaaaatga tatctttatc gaaatattat tagtaaaatc gttatattag cataaccaag
     6601 tgagcgtatg cagtaaatta aaaattagga aagtaaggag gtttgaaggt gaattcagag
     6661 tataaaaaag gcatattttt agcactcagt gcatacattc tgtggggaat actacctata
     6721 tattggcagt tcgttgatgc aataggcgca tttgaaattt tagcctttcg tattatattt
     6781 tcagcaatat tcatgatttt catactcgcg gttggacaaa aacaacgcaa tgcatttcaa
     6841 cgagatatga atcaattgtt aggcaagccc attcagctat tagcgattgt cgtagcaggc
     6901 tatgtcatta cattaaattg gggtacattt atttgggctg taacgaacgg tcacgtccta
     6961 caaacaagtt taggttatta tataaatcca cttgttagca ttttgctcgc acttatcttt
     7021 ttaaaagaaa gattcaataa atttgaatgg ctagccattt tattcgcatt catcggtgta
     7081 ttatatatga cgctcaagat tggagaattc ccaatcgtct ctattatatt agcgttatcc
     7141 tttggtacat acggattatt gaaaaaagta gtacatattg atgccatcag cagtattacg
     7201 attgaatgta ttgttaccgc acctgctgga ctaatatacg ttatttattt atggcagcaa
     7261 catcagatgt catttggatt gaacatgtca tcattttggt tgttattttc tggtgctatt
     7321 acggcaatac cactaatcct attctcagcc ggggcaaaac gtattccact ttcgctaata
     7381 ggatttattc aatacgttgg accaacaata atgtttgtac tcggcatatt tgttttcaaa
     7441 gagcctttta gtatagatca attaattacg tttatattta tttggacagg tattgtgtta
     7501 tatagtcttt ctcaatacat taaattgaag aaacatccgg tcgcaaaaac cctataaaat
     7561 acgaggaaag ataattatac gtgttttcaa tatcctttgt gtcatgatga ggtgagactg
     7621 aggtatgtgt gcagtaacag tgacagacgt ataaatttga aacattaaat atgaaataag
     7681 gcactttcgt tgaattcgca attaaaatga atttaaacga aggtgttttt tgtatgatga
     7741 tttatgaggg catacgtaat gttaatcaat gaagcggaat agtatagaaa tgtattttat
     7801 gtgatgttac gtaatgactt aaatctttat acaaattttc agtgaagcac gcaatgattt
     7861 aaaggataaa cgtattttaa aaagggagtc ttgatttagc aaaggttgat tttaaataca
     7921 aattaaaaag cgcccaaatc aatgagattc gagcgctaat ttaaatattt taaaatatac
     7981 caattacttt catccaaagt gatcctaaac caatccagat aataaagtag acgaaaccta
     8041 atattaagtt cattgtccac caacgttttt gagtcacgta acctgaagag aatagaatcg
     8101 gcgctggacc actactatag tgtgttgttg aagctaatag gttaccgaag aaacctaaca
     8161 ttaatgcact gaataatggt ggtgcaccgg ctgcgatggc aacgcctagt aatgctgcat
     8221 acatcgcact gatatgtgct gtagaacttg caaataagta atgtgaatag aagtagaaca
     8281 atattaaaat gaccaggact ataggccagc ttaagccacc aagacttgta gcaatggatt
     8341 tacttaacca aggaataaat ccaagcttgt ttaattggtc ggccattaac actaatactg
     8401 agaaccatac taatgtgttc caagcacctg tttcgtttaa aatgtcttgc catgttaaga
     8461 cgcctgtcaa taataacaat gctagcgcaa taaaggccgt taaagttgca tcaatatgaa
     8521 tgaaacttcc gacaatccat agtgttaacg caacgacaaa aataccaatc ataaattttt
     8581 cagctaaagc gattttaccc atagtcgcta attcattttc agcccaactc ttagcatttg
     8641 gtgtttcttt aacagttggt ggataaattt tataaataat aaaaggtaca acaattaagg
     8701 aaacaagtcc aggaactaaa gcagctagaa accaattcat ccatgtaatg tgaacattag
     8761 atgtgctaga tgctaaattt tgtgcaaggg ggttaccggc cattgcagtt aaaaacatag
     8821 ccgcagtaat taaattacct tggaattctg tgaaaacaag aaatgcaccc attttgcgtg
     8881 ctgatccgtc tttcggtttc gaaccaaatg attcagaaag tgatttgata attgggaaca
     8941 tgattccacc agcacgcgcg gtattacttg gtgtagcagg cgctagaatt aaatctacac
     9001 cgacgataga atatgctaat cctaatgttt ttttaccaaa taatttgacg aaatgaagtg
     9061 cgatacgtct accaagacct gttttcacaa atcctctcga aatgaaaaat gccatagcaa
     9121 ttaaccaaat gctattatta ccaaaaccag cgacagccgt tttcatgtca acaatgccaa
     9181 cgagtaccat gattgtaaat ccaattatag agacggcccc aattggcatc ggttgtgtaa
     9241 tacaagcaat gattgtcgcg acgaatattg cgaacatata ccatgctgtt ggatccacag
     9301 catccggttt aaaaggtgta agtgcccaaa taaggagacc tacaacgata gggagtataa
     9361 acttacgata tttaaccgtg ttttccatgt taaaacgtcc ttctttctat gttttataca
     9421 tatttcaatt taagaataaa gctaactaca aaagatgtac agtaataatt aaatataaaa
     9481 ttaaattaac gaaatcatta atataattat ttttcgagaa tcggtgaaga actggtatag
     9541 atggtgttta ttaaattcaa aaaagtgacc ttttaaacaa ataaaaaacc cgttaacgtg
     9601 ttgttaacgg ttaataagca catatttatt ttaaattttt taatatacga gacatctctt
     9661 caattttacc agcatgaagt gggatgtgaa tggcatttaa tactaataac tcttctaagt
     9721 tattacaacc gacaattggc tgtttcaatt caattgctaa atcatcttca gttagatttc
     9781 gtgcacgttc aggtaaagtt tggagacctt ctaaaatcct ttcaatactc ggtacttcat
     9841 ccttccattc atctggagac gaaccatttc cgaataaagg tctatagata tttaaatcaa
     9901 tattctcttt accagcaaca gctaaggccg attcaaaaat tgttaataca tgaccaaatt
     9961 gccaatgaat ggtattagaa aaaggcttat gataatcatc aagtaccttt tcaactttcc
    10021 aagcatcata cacatttact aaatatccta accccatatc aatgacatca aatactgttt
    10081 ttgtagtcat tattaattcc tcccccataa ataaaatata cttccgtaag tattacccta
    10141 ttaacaaagt tgttagtcat ttaaggaatg tgtacaacaa ctaccttaca ataaaagata
    10201 gttgacgaaa agttctcaat gtactatctt attatgtaag atagtaggtg attaaaatgt
    10261 cagtgtcaga tcagattatg aaaggtctct tagatggggc gatattaggt ctcattgccc
    10321 aaggcgaaac gtatggatat gaaattatga cgaaacttaa gaatcaagag tttccagaga
    10381 taagtgaggg tagtatttat ccagttttaa tgcgtttaaa caaaaaagga tacgttgata
    10441 cgacaataaa aaaatctagt aatggcggcc cgaggcggaa atactatacg attaccgatt
    10501 caggtttaaa ggaactaaaa gtatttaaat cgaaatggca agttttgaat aaagggatga
    10561 ttaacttgtt tggaggggaa aacgatgtta agtaaacaat ctcaagattt tttagaaaaa
    10621 ttacgtgttg aattgttatt tagaggtaag actgaagcag aagtagaaga gttattggaa
    10681 gagctcgaag atcatttaac tatggctgaa aaaaataacg aagatacaag ttcaattatt
    10741 aatacaccga ttaagtcaat ggctgatcaa ttatcacctg agataagtct tactaccggt
    10801 ttgtataaat atattacttt gtacttcgct tttattttag cagtcattat aattcctagg
    10861 tttttagatt tgggtacatt tgatgtgaca attgcatttt tactttatat agctagtatc
    10921 gttattttag gtgttgtcat tggtatgctg attctacgaa atacattagt ccgatttggt
    10981 gaccaaaaaa taacgtatgt tataaatgtg acttatggaa ttatcgtatt tttatggatg
    11041 attttaggtg gcttgattat taaaaaatat cctatctata gtttctttga gttaagtgac
    11101 aaacagagtt tcatcgttgg gttagtactt ttaattgcgc tcattggaac ttgtatttta
    11161 attaaacaga aaatttatgc gctcatcgtg ttggtaattt cattgccaag tttgatagca
    11221 aggatagcag ctatctttag taaagaacca gacactttta atttagtttc agtaagtata
    11281 ctcatcattc taaatatagc atttattatt tatacatttg taaattttag gaagttaaaa
    11341 aaagaagact aaacaatgtc gtttagctaa aatacatcat tatagtaaaa ctgtatacca
    11401 atcatccaaa agcaccctaa agttagactt aaactaacaa attagggtgc ttttgtttgt
    11461 gcacttataa ctattccgtt gtgcccaggg ctaggtgtaa atcctcatca ttagaaatag
    11521 cttcaatggc tgcagtcaaa cctgcaacta tcttttctaa tggcatagat ggtgtatcgg
    11581 gtttaccaat gacctgctct ggtatgtatg gcacatgaat aaatccgaat cgtaggtgag
    11641 ggtaatgctt atcttgtaaa taacctaagt gataaagtgt gtgattacaa acaaatgtac
    11701 ctgcgctatt tgaaagtgct ccaggaagtc cttgattaat aatactttga gtcattgctt
    11761 taactggtaa atttgaaaaa taagctggcg caccgtctaa gtgaatggct tgatcaatag
    11821 gttgaaaatc atcattatct ggaatacgtg catcatcaat attaatggcg acacgttctg
    11881 gggtaatggc atttctacca ccagcttgtc ctatagctag tacaacatca taatgattag
    11941 atgccaacgt tttatttata atattatcta ctttcttaaa agaggttggt agttttaatt
    12001 tatcgattgt atgtgtgcca ataatatctt ctagttgagt cacagcttcc catgagggat
    12061 tgatattttg attgtcaaaa ggcgcaaacc ctgttactaa aatgtgcatt ttaagacacc
    12121 ccgcagtatt aaaatttaat ttagtataac acgcgcataa ttaaaaacat atcaggttat
    12181 gtatgcgcat aaaaaacagc cttgaaaaac ctttataaat cggattttca aagcgtgttt
    12241 tgaaaggtat ttaattaaaa ctaagcatgt tgatgtaaat catcaaagtt tgttaaacgt
    12301 tgttgccact cattatcact aatattattt gcttgaacat aacgattacg ctcatgttta
    12361 gcacattcat aagagcatgc acctaaatat ttagtttcgt tttcttcaga aactaatatt
    12421 tgtttattac attctgggtt agcgcaatta atataacgtt cacatggttt gccatcaaac
    12481 caatccttac caataattgt tttttcaact tggttgatat caacactgat acggtcatca
    12541 aatacgtaca ttttaccgtc ccaatattca ccttttgttt caggatcttt accatatgta
    12601 gcaataccgc catgaagttg agctacatct tcgaaacctt cttttaaaag ccatccagaa
    12661 aatttttcgc atcgaatgcc accagtacag tacgtaacca cttttttatc tgcaaataac
    12721 gctttattct ctttaatcca gtcaggcaaa tctctaaaac gtgtgatatt tggacgaatt
    12781 gcacctcgga aatgacctaa atcaaattca taatcattac gtgcatcaat aatgactgtg
    12841 tcatcatctt caagagcttt tctaaattct acaggtgata aatattggcc agttgtgtgt
    12901 cttggatcga cgtcatcttc taaatctaaa gcaacgattt cttttcgagg acgtacatgc
    12961 attttcttaa aagcatgtcc ttcagcttca tcaattttaa acaccatatc tttgaatcgt
    13021 tcatcggcat gcatatgtgc catatattgt tcggtttctt cttttgtacc agataatgtg
    13081 ccgttaatac cttctgtaga aacaagaatt ctacctttta aatggtgtgc tttacaaaag
    13141 gctaagtgat cctgagcaaa ctgttcaggg tcatcaatcg tcatatattt ataatataaa
    13201 agaacttgat agttcatagg ttaatctcct tctataaaca tgttataagc atgttaattt
    13261 ttaaaataaa gcgtcaatac gcatatttgt ctacgtccat cattttaaca gagttgtatt
    13321 gtcattcaat atttctagct tgaaatcagc cactagaccg ataaaataga tagatgaaac
    13381 cgttatcatt ttatttgata gttatcttgt ttaaaataaa attgatagtt aaaacgaatg
    13441 aaatgaaagg aatgatcatg atgtcacaac aagatttacc tacattattt tatagcggga
    13501 agtccaatag tgctgttcca attatatctg aaagtgaatt acaaacaatt acagctgaac
    13561 catggcttga aatttccaaa aaaggattgc aactagaagg attgaacttt gatcggcagg
    13621 gacaactctt tttattggat gtattcgaag gcaatatttt caaaatcaat cctgaaacga
    13681 aggaaatcaa acgacctttt gtaagtcaca aagcgaatcc tgcagcaatc aaaatacata
    13741 aagatggccg attattcgtt tgttatttag gagattttaa atctacagga ggcatttttg
    13801 cagctacaga aaatggtgac aacttacaag atattattga agatctttca acagcatatt
    13861 gtattgatga catggtattt gattctaaag gtggatttta ttttacagat tttagaggat
    13921 actctaccaa tccactagga ggcgtttatt atgtttcgcc ggactttaga acagtgacgc
    13981 ctatcattca aaatattagc gtagcaaatg gtattgcttt aagtacagat gaaaaagtac
    14041 tatgggtaac agaaacaaca gccaatcgat tacatcgcat tgcacttgaa gatgatggtg
    14101 tgacgataca accatttgga gctaccatac cgtactattt tacaggtcat gaaggaccag
    14161 actcatgttg tattgatagt gacgataatt tatacgtagc aatgtatggt caaggtcgag
    14221 tgttagtttt taataaaagg ggttatccaa taggacaaat attgatacca ggccgagatg
    14281 aagggcatat gttacgttct actcatccgc aatttatacc tggaacaaat caactcatca
    14341 tttgttccaa tgatatagaa atgggcggag gatctatgct ttatacagtt aatggctttg
    14401 caaaaggtca tcaaagtttt cagtttcaat aagacctacc tgaatgaaag gcgtctaaaa
    14461 ttagggtgaa cagttagtgc ttgttacaca tgagttaaac gaaaaatgcc aactatacct
    14521 agggatgata tagttggcat ttcgtatata tattaaaaca atgaggattg tgtaatgaga
    14581 ttattctgag attgagaatt cagctgatgc ttcgaattta acatctttgc ctagcattac
    14641 gccaccagtt tcaagtgctt ggttaaagtt aatgccatat ttttctctat tgattgtacc
    14701 agtaacaata atacctgtta cttgagaacc atccatagga ttcttactta ctccgttgaa
    14761 ttcaacatcg aatgtttctt cgttagtgat gcctttaatt attaaatcac caacaacttt
    14821 gctttcagat actgattttg tcacaaatgt aattttatca aattcgtcag taccaaagaa
    14881 atcaccagat tttaagtggt tatcacgtgc ttcgttttta gtgttaattg agcttggaat
    14941 aattgttgca gtagctttca aagtactgaa gtcattaata tctccttcaa cagctacatc
    15001 aaattgatca aatgaacctt tcactttaga aaccattaaa tgtttaattt ggaattctaa
    15061 actactgtgt gcaccatcaa aagtaaagtt agtcataata taaaacctcc atatttttat
    15121 ctcttagata tattttttat acttgatata catacaatac cacctaaaaa acaaagatgc
    15181 aagcatttta tttcgaattc gaatattttc tgtgatgtat ttaaagaatg acctataaaa
    15241 caccacacta ttgttatacc ctagtcaatt aattcaaaca taaagaagtg attacaagtc
    15301 cgtgaaattt agttaatgag ataaatctga ctattattat catttttata gtcactacta
    15361 ttgactttgc atcaagtgac tcgtactatt ttaatataat tctgaaaatt caaaatatag
    15421 aacattaaat atgtagataa tatttttttg aagttttaaa aaaaggaggc attttatggg
    15481 aaattatttg gtattgcaat cagattttgg acttagtgat ggcgctgtaa gtgcaatgta
    15541 tggagttgca tatactgtaa gtgatgatat acgtgttgaa aacttaacgc atgatatacc
    15601 accgtatgac atttgggtag cgtcatatcg tttatatcaa actgtaaaat actggcctaa
    15661 aggcacagtt tttgtatcag tggtagaccc gggtgtaggt agtgataggc ggagcattgc
    15721 ttgcttaaca tattcaggtc attacattat tacacctgat aatggttctt taagtcatat
    15781 taaacattac gaaggtatta aaaaagttat cgaaattgat gaagtgaaaa gccgattgcc
    15841 tcattcggaa gaaagtcata cttttcatgg tagagatgtt tatgcataca atggtgcgag
    15901 attagctgca aataagatag cgtttgaacg tctcggtcag gcaattaatg ttgatagtat
    15961 tgaggcatta gaaataagag aagtgacaaa aaatgaggat tctgttacag gaagcattga
    16021 tattttagat atcagatttg ggtcattatg gacaaatatt ccattggcat tcttaaaatc
    16081 cttggaaatt gttcatggta ataatttggt ggtaacaatt tatcatcaag ataagaaagt
    16141 ctatcaaaat attattaaat ttgcgcgatc gtttgcagat gttaatgtgg gtgaacctct
    16201 tgtgtatgtt aactcgctag taaatattgg tgtagcagtc aatcaagact cattttcaga
    16261 tttatatcat attggtactg gaaatgattg gacgattcat ttatctaaag caccatcaat
    16321 ataatgaatt ttttatgtaa ggagtaatga agatgaaaaa acaagatatt tcagttaaaa
    16381 ctgttgttgc aattggtata ggagcagcgg tatttgtaat tttagggcgt tttgttgtaa
    16441 taccaacagg ttttcctaat acgaatatag aaacatcata tgcattttta gcattaatat
    16501 ctgcaatatt tggacctttt gctggtttaa tgactggatt agttggacat gctattaaag
    16561 attttacgac atacggtagt gcctggtgga gttgggttat ttgctcggga attataggct
    16621 gtttatatgg ctggattgga ctaaaattaa atctttcttc aggccgattt tctaggaaat
    16681 cgatggttta ttttaatatc ggtcaaatta ttgcgaatat tatttgctgg gcacttattg
    16741 caccaacatt agatattttg atttataacg aaccagctaa caaggtttat acacaaggtg
    16801 ttatctctgc agtattaaat attatttcag ttggcattat tgggacaata ttattaaaag
    16861 catatgcttc atctcaaatt aaaaaaggta gtttacgtaa ggaatagtta ttttgttgaa
    16921 tcagatatgt aaatgaatat agaaaggtaa tgatatatca tgactgaacc aattatctcg
    16981 ttcaaagact ttagttttca atatcatagt caagcaacac ctacattaca gaatataaat
    17041 gttgatattt atccaggaga aaaagtatta gtagttggtg cttcgggtag tggtaaatcg
    17101 acttttgcaa attgcataaa cggattaatt ccatttaaaa ctaaaggtaa cataactggg
    17161 gaactatata taaataatca agatgcaacc gttagttgtt tacatgatag atctaatgtt
    17221 gttggtacag ttttaaaaga tacagatgga cagttcatag gcttaacagc agctgaagat
    17281 atggcctttt tattagaaaa taattgtgtt gaacaagatg atatgaagaa aaatgtaagt
    17341 tattgggctg aaaaagttgg catgatagaa catttaaatc accgaccgca agatttatct
    17401 ggaggtcaaa aacaacgcgt ttcattaggt ggtatattaa tccatcgtac gcctatttta
    17461 atattggatg agccactggc caatttagat cctgcgacag gacatgaaac gctgagattg
    17521 ttaaacaata ttcatgaaga aacaaagtca acgatgatta ttgtcgaaca tcgattagaa
    17581 gaatcattag atgatacgtt tgatagagtt cttcttttta aagatggaaa aatcatcgct
    17641 aatacaacgc ctagtgattt gctaaaatca tctaagctta aagaagcagg catacgtgaa
    17701 ccattatact gtacagcgct taaatatgca gaagtagatg tcgaatccat tgataactta
    17761 gcgaatttac gtgacgtttg tatgagtgaa catgttaaat ttaaagtgaa aaaatggata
    17821 gatgaaacat ctgcaaataa tgataataag tacaaatctg aaccgctatt agaattaaat
    17881 gaggtatgtg ttcaatatag tgactatagt aatagtgtac ttaataatgt tcaattaaat
    17941 gtttatagaa gagaaatgct tagtatagtt ggtcacaatg gtgccggcaa atcaacactt
    18001 gctaaagcaa tttgtggatt tttagatata acaggtaata tccaattttg taatagggga
    18061 tttaatcaat tgtcaattag cgaacgatct gaattcgtag gttatgtgat gcaaaatcca
    18121 aatcatatga tttctgaaaa aatgatttac gatgaagtag cattagggtt aagagcacga
    18181 ggtatgaaag aatcagatat aaagatacga gtagagaatg tgctcaaaat atgcggactt
    18241 tatgcatttc ggaattggcc aattgcagct ttaagctatg gtcaaaaaaa gagggtcact
    18301 atagcatctg ttttagtctt aaatccggaa ataatcatat tggatgaacc gactgctggt
    18361 caagatttct atcattataa tgagataatg tcatttttaa ttgaactaaa cagacagggg
    18421 aagacgatta ttatgattac gcatgatatg catttattgt ctgagtatag ttcaagaaca
    18481 gttgtattat caaaaggtca agtcgttgct gataccacgc cagtattggt tttaaatgat
    18541 aaaaaaatct gtgagattgc atcattgaga caaacatcgc tatttgaaat ggccgaatat
    18601 atagggatta gcgagccaca gaaattagta caattattta ttaaccatga taggaaggtg
    18661 agacgccaat gaatcaatat aatactatag gttttcaccc gggaaatagt cgtattcatc
    18721 aattaaatgc gactgttaaa cttttattct tattagttgt ttctatttct gcaatggtga
    18781 cttatgacac aagatattta attttaatta gtgcttcatc tattttattg gtcaaatatg
    18841 ctcatattga atggaaacaa gttcgctttg ttgttaaatt cattctgttt ttcacaatat
    18901 taaatattat tgccgtgtac atatttgacc ctgaatatgg tgtgaagatt tataatcagc
    18961 gtacagagtt agtcaatggt attggtcgat ttacgctaac atcacaggaa ttattctatc
    19021 tttttaatct aatattaaaa tatattagta cagttccttt agcgttaata tttttattca
    19081 caacgaatcc gagtcatttt gctgcaagtt taaatcagct aggtgtgaat tataaaatca
    19141 gttacgcagt ctcactagca ttaaggtata ttccagatat tcaagaaaca tattttaata
    19201 tttcacaagc gcaacaagca agaggatatg ataattcgaa aaaagcaaaa tttactagcc
    19261 gtgttaaagg tatcaaacgc attgtgttac ctttaatatt ttctagtatc gaaagaattg
    19321 acactattag tactgctatg gagttaagac aattcggaca gtataaaagg agaacctggt
    19381 acgtcaaaaa acaattaaaa aaagatgatt atgttgtttt gtgtttgacg ttaatacttc
    19441 tgatgttagt agttacatta ttctttttaa ataatagtcg atatttcaac ccgtggcatt
    19501 agtattcata taaatagtct ttaaatagaa ataggaggga gacatttaat gataaatact
    19561 gaaagattaa atttaatgat tccaagttcc tcgcatttaa ttgaacttta taatatttgt
    19621 agtcatccac aagcaaatat atacactccc aaaggtttac ataattccaa attagacaca
    19681 caacggtgga ttgaaaaatg gcgaaaccat tggcaacaat atcaatttgg ttactttgta
    19741 ttggtaaaaa aaatagattg tagtgttatt ggtatttgtg gatatgaata tcgacaatta
    19801 aagcaagaaa cagtattaaa tttattttat aaattacatc caagttttga aggacaaggg
    19861 tacgcatgtg aggctattac agcaatcaca aattttgtga attatatcga tcaagaaaca
    19921 gtaaaagtta tcaggacaaa taagtgtaac caacgttcaa taaatttagc agaaaggctt
    19981 aaattcaagc gagacgatac tatggacgac attatcaatc aaggagatat tgtgttttat
    20041 aaataaaata ctatgacatt atctaaaaaa taaaattaaa atcccaacag tttcttaaaa
    20101 agttatgagc cacataataa aaatgttata attgtgcata ataaaacata gggagaatgt
    20161 ttaatgcata aaaaatattt attgctattt tttctgttat tatcagttgc attgttatat
    20221 agctgttcca agcaaaatga gaaagctcaa aataaatctg ttaatccaaa tgagtatgca
    20281 aagcataaat cgaaaatgga tgaacattgg gaaacatatc acggccaagt ttatcatgtg
    20341 ttttatcacc cattaattac agacccaaaa gttgcgttta caggagatac acaccaagct
    20401 aagggcaaca atgattggat gattactgta agtgaattta aaaaatcatt gaacgaatta
    20461 tataaaaata attacatcat cgttaatcca catgatgtat acgacttatc atctcatcat
    20521 gtaaaagcca aaaaaataaa attgccgaaa ggtaaaaagc cactaatcat ctcaattgat
    20581 gatatgaatt actatagtta catgagacac catggttatg ctgaccgttt agtactcgat
    20641 aaaaataaac atgttgtttc tgaaactaca gacaaaaaag gccatgctac ttattctgat
    20701 gacaatgata tcgtgccaat tttaaataaa tttgtaaaag agcatccaga tttttcttta
    20761 aatggtgaaa aaggtgttgt tgctttaacc ggttatgagg gcgtattagg ttatcgcacg
    20821 aatgaattaa cgagtaaaga ttatacaaag aataaaaagg cagctgagga agttgttcgc
    20881 gcaatgaaac gggatggatg gtcatttgcg agtcattcat atggtcacat taattttgaa
    20941 aaaacatctt tagaaggtat taaacgtgat actaaaagat ggaaagatga agttgaacca
    21001 attgtaggta aaacagatat gtttgtattt ccacatggcg cacaggatag acatacacaa
    21061 gcgtatgatt atttggtaga tgaagcagag tttaagttta ttgcaggtgt cggtccaaac
    21121 aattttacag atatatcagc aacgaacgtt taccaagata gggttgcgat tgatggtcta
    21181 aacttatttg aatttaaata taaactcaaa ccgtttttta atcctgaaaa tgtatatagt
    21241 aaacaagata gacgttactt taaagggaat cgggattatg aagaatagtt aaatttaata
    21301 aaaagataga aagaaaataa ctttacataa taaggcaagg acaatatgtg caagaagtac
    21361 aacttcaata taaaaatctt ggttaagcat ttttataggc acaaatgtct tgcctttttt
    21421 attttaaaaa attttaaaat caatacaccg aattttctga aaaaggttgt aatgtattgc
    21481 taaaaatgat aagttattaa tcatattaca ctttagcgtg gtagagaagt aaagcaaaca
    21541 acgaaaatac caagtgattt taaatacagt attgaaagta aagggtggtc gagtgaataa
    21601 ttcagaacaa ttaattgcgt taaaagaatc ggaaacagca tttttaaaat attttaataa
    21661 agcggattat gagttagttg attttagtgt ggttgaaaag ttggactgga aacaattgaa
    21721 ccatgaggat ttgcagcaga tgggtgagcg aaatttttgg caacatgagc atcaaattta
    21781 tgcacttcga aatgatttta cagatcaatt attgcgttat tactcaatgt atccaactgc
    21841 agcaacaaaa gtggcataca caggtttaat cattcggaat aatgaagcgg ctgtacaagt
    21901 tggtttggaa aactatgcac catcattagc gaatgtgcaa cagagcttaa agctctttat
    21961 tcagtttatt caacagcagt tgagagacaa cgtgcatttt gttgtattgg gacattatca
    22021 attgcttgat gccttactcg ataaatcgtt acaaacacca gacatactat caatgattga
    22081 ggaacgtaat ttatcgggac tggttacata tttgtcgact gaacatccaa tcgtgcaaat
    22141 cttgaaagag aatacgcaac aacaattaaa tgtattggaa cactatattc caaatgacca
    22201 tccagcactc gttgaattaa aaatatggga acgttggtta cataaacaag gttacaaaga
    22261 catccattta gatattactg cgcagccacc tagatcctat tacacaggtt tatttattca
    22321 atgtcatttt gctgaaaatg aatctcgagt tttaacaggt ggttattaca aaggaagcat
    22381 cgaagggttt ggattaggat taacacttta agtaagggag tatgcacaat gttaagaatc
    22441 gccatagcca aaggacgtct aatggatagt ttaattaact atttagatgt aattgaatat
    22501 acgacattat cagaaacatt aaaaaataga gaacgccaat tattattaag tgtagataat
    22561 attgaatgca ttttagtaaa aggaagtgac gtgccaatct atgtggaaca aggaatggca
    22621 gacataggca ttgttggtag cgacatatta gatgagcgcc aatataatgt taataatttg
    22681 ttgaatatgc cttttggagc atgtcatttt gcggttgcag cgaaacctga aacgaccaat
    22741 tatcgtaaaa tcgcaacgag ttatgttcat actgctgaaa catattttaa atcaaaaggt
    22801 attgatgtcg aattgattaa attgaatggc tctgttgaat tggccggtgt tgtagatatg
    22861 gtagacggaa ttgtcgacat cgttcaaaca ggtactacgc taaaagcgaa cggactggtt
    22921 gaaaagcaac atattagtga tatcaatgca agattaataa ctaataaagc agcttatttt
    22981 aaaaaatcac aattaataga gcaatttatt cgctctttgg aggtgtctat tgccaatgct
    23041 taatgcacaa caatttttaa atcaattttc attagaagca ccattagatg agtcattgta
    23101 tccaattatt cgcgatattt gtcaggaagt taaagttcat ggagataagg ctttgaaaat
    23161 gtataatcta acattcgatc atacgaaaac agatcattta gaaattagtc atgaacaaat
    23221 taaagcagca tttgacacat tagatgaaaa aacaaaacaa gcattacaac aaagttatga
    23281 aagaattaaa gcatatcaag aaagtattaa acagacgaat caacagttag aagaatcagt
    23341 ggagtgttat gaaatatacc atccactaga aagtgtcggt atttatgtgc ctggtggtaa
    23401 agcaagttat ccttcaacgg tattaatgac agcgacgtta gcacaagtag caggtgtgga
    23461 aaatattgtc gttgtgacac cacctcaacc taatggtgtg tctcaagaag tattagcagc
    23521 atgctatatt acgcaagttg atcaagtatt tcaagttggt ggtgctcaaa gtattgctgc
    23581 gttaacttat ggcacagaaa cgatacctaa agttgataag attgtaggtc caggcaatca
    23641 atttgttgca tatgccaaga aatatttatt tggacaagta ggtattgacc aaatagctgg
    23701 accaacagaa atagcactta ttattgatga aacagcagat ttagatgcca tcgtatatga
    23761 tgtttttgca caagcagaac atgatgaatt agcacgtaca tatgccatta gtgaagatgc
    23821 gcaagtcctt aaagatttag aatcacgtat tgctaaagca ttgcctaatg tggacagata
    23881 cgacattgtt tctaaaagta tcgctaatca acactacctt atccatgcta gtaattttga
    23941 tgaagcatgc catgtcatga atacaatcgc gcctgaacat gcgtcgattc aaacagtaaa
    24001 tcctcaacca tatattgaaa aagtgaaata tgtgggtgca ttgtttattg gacattattc
    24061 gccagaggtc ataggagatt acgttgcagg tccaagtcat gtattaccta caaatagaac
    24121 agctagattt accaatgggt tatcggtcaa tgatttctta acacggaaca cggtcatcca
    24181 tttatcaaaa gatacgtttg aacaaattgc tgattcagca caacatattg ctcatgttga
    24241 agcattatac aatcaccagc agtctatttt aatacgtcag tcttagggga gtgtaattga
    24301 aatgatttat attgataaaa atgaaagtcc agttacgccg ttggatgaaa aaacaatgac
    24361 gtctattatt agtgcaacgc catataattt atatcctgat gcagcatatg aacaattcaa
    24421 ggaagcttat gctaagtttt acggattatc gcctgaacaa attattgcag gaaatggatc
    24481 tgatgaattg attcaaaagt taatgctgat catgccagaa ggtccggcat taacgctaaa
    24541 tcctgatttt tttatgtatc aagcatatgc ggcacaagta aatcgtgaaa ttgcatttgt
    24601 agatgcagga tcagatttaa cgtttgattt ggaaaccatt ttaacgaaaa tcgatgaagt
    24661 acaaccatca ttttttatta tgagtaatcc acataaccct tcaggcaagc aatttgatac
    24721 ggcattttta acagctattg cagataagat gaaagcatta aacggatact ttgtcattga
    24781 tgaagcatat ttagattatg gtacggcata tgacgtggaa ctggcaccac acatcttaag
    24841 aatgcgtaca ttatcaaagg cgtttggaat tgccggctta agattaggtg tcttaattag
    24901 tactgctgga acgataaagc atattcaaaa aatagaacat ccatatccat taaatgtatt
    24961 tacgctaaat attgcgactt atatttttag acatagagaa gagacaagac aatttttaac
    25021 gatgcaacga cagttagctg agcagttaaa acaaatattt gatacacatg ttgcagataa
    25081 aatgtcagtg ttcccatcaa atgctaattt tgtacttact aaaggctcag cagcgcaaca
    25141 attaggacaa tacgtatatg aacaaggatt taaacctcgc ttttatgatg agccggtgat
    25201 gaagggctat gtaagatact caattgcaac agcatcacag ttaaagcaat tagaagaaat
    25261 tgttaaagaa tggagtgcaa aatatgattt atcaaaaaca acgaaacaca gctgaaacgc
    25321 aactaaatat ttcaatatct gatgatcagt caccatcgca tattaataca ggtgtgggct
    25381 ttttaaatca tatgttaacc ttgtttacat ttcatagcgg tctgtcatta aacattgagg
    25441 cacaaggtga tattgacgta gatgatcacc acgtaactga agatatcggc attgtcattg
    25501 gccaattgtt acttgaaatg attaaagata aaaagcattt cgttcgttat ggaacgatgt
    25561 acattccaat ggatgaaaca ttagcacgtg tcgttgtgga tataagtggg cgcccatacc
    25621 tatcattcaa tgcatcatta agtaaagaaa aagttggtac gtttgatacg gagttagtag
    25681 aagaattttt tagagcggtc gtaatcaatg caagattaac aacgcatatt gatttaattc
    25741 gtggaggtaa tacacaccat gaaattgaag ctatattcaa agcgttttcc cgtgcattag
    25801 gcatagcgct aactgcaact gatgatcagc gtgtgccgtc atcgaaaggt gtgattgaat
    25861 gattgtcatc gttgattatg gattagggaa tattagtaat gtaaaacgcg ctattgaaca
    25921 tttagggtat gaggtggttg tctcaaatac ctcaaaaata atcgatcaag cagaaacaat
    25981 catattgccc ggtgtcggcc attttaaaga tgcgatgtca gagataaaac gattaaatct
    26041 caatgcaata ttggctaaga atactgataa gaagatgatt ggtatttgtt taggcatgca
    26101 attaatgtat gagcatagtg atgaaggcga tgcatctgga ttagggttta tcccaggaaa
    26161 tatttcgcgt atccaaacag aatacccagt gccacactta ggctggaata atttagtgag
    26221 taagcaccct atgttaaatc aagatgttta tttcgtacat tcttatcaag cgccgatgtc
    26281 agaaaatgta attgcatatg cgcagtatgg ggctgatatt ccggcaattg ttcaatttaa
    26341 caattatatt ggtattcaat tccatcctga aaaaagcggt acatatgggt tacaaatttt
    26401 gcgtcaggca atacaagggg gatttataaa tgattgaatt atggccagcg attgatttga
    26461 ttgggtcaac aagtgtgagg ttaacagagg gtaaatatga tagtgaagaa aaaatgtcac
    26521 gctcggctga agaaagtatt gcttactata gtcaatttga atgtgtgaat cgtattcata
    26581 tcgtcgactt gataggtgct aaggcacagc atgcccgaga gtttgattat attaagtcat
    26641 tgaggagatt aacaaccaaa gatattgaag taggtggtgg cattcgtacg aagtcacaaa
    26701 tcatggacta ctttgccgca gggattaatt attgcatagt tggaacgaaa ggtattcaag
    26761 atactgattg gcttaaagag atggcacata catttccagg tcgcatttat ttatctgttg
    26821 atgcctatgg agaagatatt aaagtgaacg gatgggaaga ggacacagag ttaaatttat
    26881 ttagttttgt gagacggtta tcggatatac ctcttggcgg cattatatat actgatattg
    26941 ctaaagatgg caaaatgtcc ggacctaact ttgaattaac tggtcaatta gtaaaggcaa
    27001 cgacgattcc tgtcattgct tccggtggta ttagacatca gcaagatatt caacgattag
    27061 cgtcgctaaa tgttcacgct gctattatag gaaaggctgc acatcaagca tctttttggg
    27121 aggggctaaa atgattaaaa aacgtatcat tccatgttta gatgtcaaag atggtcgtgt
    27181 cgttaaaggg attcaattta aaggattaag ggatattggg aatcctgttg atttagcaat
    27241 gtattacaat gaagcgggtg ctgatgaatt agtattttta gacatctcta agacggaaga
    27301 gggtcatagc ttaatgctag aagtgattga acagacagcg tcacgcttgt ttatccctct
    27361 tactgtaggg ggtgggattc aaagtctcga tgatattacc caattgctaa atcatggtgc
    27421 agataaagta tcattaaatt caagtgcttt aaaaaatcca cagctcatta aacaagcgag
    27481 tgataaattc ggtagacaat gcatctgcat agcaattgat agctattatg atcctgaaag
    27541 aaaagcacat tattgttgta cgactggtgg taaaaaaatg acaaatatta aagtatatga
    27601 ctgggtacag caagtagaac agttaggtgc aggtgagctc ctcgttacaa gtatgggaca
    27661 tgatggtatg aaacaaggct ttgatattga acacctagca aatattaagt ctcttgtaaa
    27721 tattccaatc attgcttctg gtggtggtgg caatgcacaa cactttgtag aattatttga
    27781 tcagacggat gtttctgcag gtttagctgc aagtatatta catgatcgag aaacgacggt
    27841 tcaatctatt aaagaagtga tacggcaagg gggtatagca gtaagatgac caaatataaa
    27901 attgatttta gcaaaggttt agtgccagca attttacaag ataatcaaac aaaacaagta
    27961 ttgatgttgg gttatatgaa ccaagaagct tttgataaaa cgatagaaga tggtgtggta
    28021 tgtttctatt cgcggtcgaa acaacggcta tggacaaaag gtgaaacatc tggtcatacg
    28081 caacgtgtta aagatattca tgtagattgc gacaatgaca ctattttaat tgatgtcata
    28141 ccaaatggac caacatgtca tacaggcagt caaagttgtt tcaacacaga agttccattt
    28201 tcagtgcaaa cattagcgca gacagttcaa gatagtgccc aatccaataa tgaaaagtca
    28261 tatacaaaat atttattaac agaaggtata gaaaagatta caaaaaaata cggtgaagaa
    28321 gcttttgaag tcgtaattga agcaattaaa ggtgacaaaa aagcatttgt aagtgaagta
    28381 gcagatgaac tttatcattt atttgtcttg atgcatgcgc ttggcgtcga tttttcagaa
    28441 attgaggcgg aattagcgcg tagacatcat aagcgcaata actttaaagg tgaacgacaa
    28501 aatatcgaac agtggtaaag caagtatgga ctaagatata aggaaaagga tcatggctta
    28561 tacacttaca aatattgtgg aaaacgtgac attttcaagt ttaaaatacg acaccaacat
    28621 attttaacta tgaatgctgt gatggtacta aagttgcgaa ctcgttatag ataagtagtg
    28681 gataatcaca atacgaaatc aaaaataatt ataaaaagta aattgagcaa ctcaggaata
    28741 gatgtcactg ttaaagatgt cgaaaagtat atgaatcgat ataatgaagt tatgaaggga
    28801 aaaaatggcg aaaaagctaa agagttatgt ttgtcgttac tacctattaa tatcatagtt
    28861 gtctttacat tctttgtatt tatactataa atacaaatat atctagcctg aaatagaaat
    28921 gtcatagcct atttaaaaga caatctccat tagaactaag atatgcatcc cgaaagttag
    28981 actaaaaaac taactttatg ggatgtattt ttatgctaat catcataaat tcgagattaa
    29041 gttaaaggta gttcaagagt aattaaacaa taaattaaaa atagtaggat acttactttg
    29101 agggaagaaa attaactgta tatatttagt ttaggaacaa gtattacggt ttatcctgat
    29161 acaattattg tggatgggat gatattttta ggtttaaaat acgacaccag caaacataat
    29221 aactgtaata gctcataaat ctccccatat agctaatcta aaaaaataat acatcattgg
    29281 aattaagccc caagcatgta aatattaaaa atcaaaaaag atatatg
//
LOCUS       DAMQYT010000013        27909 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMQYT010000013 DAMQYT010000000
VERSION     DAMQYT010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 27909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27909
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.013"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(127..1200)
                     /locus_tag="PGA33_000488"
     CDS             complement(127..1200)
                     /locus_tag="PGA33_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDG6472327.1"
                     /translation="MAMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKNNWLSIIVVSISILLIAIVMA
                     FVFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYIIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(1234..1335)
                     /locus_tag="PGA33_000489"
     CDS             complement(1234..1335)
                     /locus_tag="PGA33_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472328.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            complement(1396..2682)
                     /gene="hemL"
                     /locus_tag="PGA33_000490"
     CDS             complement(1396..2682)
                     /gene="hemL"
                     /locus_tag="PGA33_000490"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDG6472329.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGNDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            complement(2730..3704)
                     /gene="hemB"
                     /locus_tag="PGA33_000491"
     CDS             complement(2730..3704)
                     /gene="hemB"
                     /locus_tag="PGA33_000491"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDG6472330.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            complement(3707..4375)
                     /locus_tag="PGA33_000492"
     CDS             complement(3707..4375)
                     /locus_tag="PGA33_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDG6472331.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            complement(4397..5323)
                     /gene="hemC"
                     /locus_tag="PGA33_000493"
     CDS             complement(4397..5323)
                     /gene="hemC"
                     /locus_tag="PGA33_000493"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDG6472332.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQNEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            complement(5365..6180)
                     /gene="ccsA"
                     /locus_tag="PGA33_000494"
     CDS             complement(5365..6180)
                     /gene="ccsA"
                     /locus_tag="PGA33_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDG6472333.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            complement(6202..7548)
                     /locus_tag="PGA33_000495"
     CDS             complement(6202..7548)
                     /locus_tag="PGA33_000495"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDG6472334.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
     gene            complement(7765..8355)
                     /locus_tag="PGA33_000496"
     CDS             complement(7765..8355)
                     /locus_tag="PGA33_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDG6472335.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
     gene            complement(8509..9771)
                     /gene="clpX"
                     /locus_tag="PGA33_000497"
     CDS             complement(8509..9771)
                     /gene="clpX"
                     /locus_tag="PGA33_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDG6472336.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            complement(9922..11223)
                     /locus_tag="PGA33_000498"
     CDS             complement(9922..11223)
                     /locus_tag="PGA33_000498"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDG6472337.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            complement(11271..11381)
                     /locus_tag="PGA33_000499"
     CDS             complement(11271..11381)
                     /locus_tag="PGA33_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472338.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            complement(11386..12315)
                     /locus_tag="PGA33_000500"
     CDS             complement(11386..12315)
                     /locus_tag="PGA33_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472339.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLADFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLK
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            complement(12334..12942)
                     /locus_tag="PGA33_000501"
     CDS             complement(12334..12942)
                     /locus_tag="PGA33_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDG6472340.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            complement(13084..13440)
                     /gene="rplT"
                     /locus_tag="PGA33_000502"
     CDS             complement(13084..13440)
                     /gene="rplT"
                     /locus_tag="PGA33_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDG6472341.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            complement(13487..13687)
                     /gene="rpmI"
                     /locus_tag="PGA33_000503"
     CDS             complement(13487..13687)
                     /gene="rpmI"
                     /locus_tag="PGA33_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDG6472342.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            complement(13716..14243)
                     /gene="infC"
                     /locus_tag="PGA33_000504"
     CDS             complement(13716..14243)
                     /gene="infC"
                     /locus_tag="PGA33_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDG6472343.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     misc_feature    complement(14272..14397)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(14472..15965)
                     /locus_tag="PGA33_000505"
     CDS             complement(14472..15965)
                     /locus_tag="PGA33_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDG6472344.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     gene            complement(16391..18328)
                     /gene="thrS"
                     /locus_tag="PGA33_000506"
     CDS             complement(16391..18328)
                     /gene="thrS"
                     /locus_tag="PGA33_000506"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDG6472345.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            complement(18526..18648)
                     /locus_tag="PGA33_000507"
     CDS             complement(18526..18648)
                     /locus_tag="PGA33_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472346.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLKQRYLIHKLY"
     gene            complement(18741..19661)
                     /gene="dnaI"
                     /locus_tag="PGA33_000508"
     CDS             complement(18741..19661)
                     /gene="dnaI"
                     /locus_tag="PGA33_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDG6472347.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            complement(19661..21061)
                     /locus_tag="PGA33_000509"
     CDS             complement(19661..21061)
                     /locus_tag="PGA33_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HDG6472348.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSAGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            complement(21062..21532)
                     /gene="nrdR"
                     /locus_tag="PGA33_000510"
     CDS             complement(21062..21532)
                     /gene="nrdR"
                     /locus_tag="PGA33_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDG6472349.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            complement(21742..22767)
                     /gene="gap"
                     /locus_tag="PGA33_000511"
     CDS             complement(21742..22767)
                     /gene="gap"
                     /locus_tag="PGA33_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDG6472350.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            complement(22937..23560)
                     /locus_tag="PGA33_000512"
     CDS             complement(22937..23560)
                     /locus_tag="PGA33_000512"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDG6472351.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
     gene            complement(23576..24448)
                     /gene="mutM"
                     /locus_tag="PGA33_000513"
     CDS             complement(23576..24448)
                     /gene="mutM"
                     /locus_tag="PGA33_000513"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDG6472352.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            complement(24464..27094)
                     /gene="polA"
                     /locus_tag="PGA33_000514"
     CDS             complement(24464..27094)
                     /gene="polA"
                     /locus_tag="PGA33_000514"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDG6472353.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            complement(27388..>27909)
                     /locus_tag="PGA33_000515"
     CDS             complement(27388..>27909)
                     /locus_tag="PGA33_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472354.1"
                     /translation="VNVIVCGYFAFSIFVGIYLSINDRKTQITLYSLLIAICVSASTL
                     VIVTPIEPGNFLIVYTIHVIILIILLKEFRKYKSINVNFIKGASLVLAVIYLSAFIYV
                     HYEHEKRVQLLKQQIKDHPRQEVYTVESLPFEHYMHHPSPINKNYQEWFNNYEELPKM
                     TKVKYIPFGSDDS"
BASE COUNT         8664 a         5331 c         3785 g        10129 t
ORIGIN      
        1 aaagttccaa gttaatgctc tgctaatatt agatatctta atctaaaaat agatgtacaa
       61 cctttgctta aaccttaata atgaattcta tcctaagcat aattcaactt agatggatga
      121 tagtttctaa tcttcacgct tattcgaacg atattttaaa aagtaattaa ttcctggtgc
      181 aataataaat aggatgaaaa agattcggaa aatatgataa ctcgtaatca tagcaacatc
      241 ggcaccagta gctaatgcaa ctaaaactat ctgattaacc cctcctggtg ctgcaccaag
      301 aaacaattca ttaataggat tattatcaaa gaaatgtatg atataaacca tgattagcgc
      361 accaattatc aacataatat tttgaattgt aattgcgatt gctagtctac cttttaaatc
      421 tgacaataaa tgcgcaattt gaactccaat tctaatcata tatattagtt gtgccatgtt
      481 caacaaccaa tgatctagtg taaatgttaa acctgtagaa aaattccaaa caattaatac
      541 aatgagtggt gctaataatt gaaatgttgg aaactttatt ttagacataa ttagataaac
      601 tataaagata gctatcgcta aaataactat ttgccctatg tttaatactt gtgataaagg
      661 caagactttt gttaactttc cattcgcatg catgttacca tcatgaaaaa aatatgaaat
      721 gaacggtact aaaacaacaa caaatataat tcgtgatgtt tgcgttaagc taacaactaa
      781 caaattagca cgtttgtctt gttcagccat gaccagcatt tgtgttagtg ctcctggtat
      841 aacacttaaa atagctgttt ctgtattaat acgtgcaatt tttttaaaaa caaatgccat
      901 tactattgca attaataata tcgaaataga tacaacaata atcgaaagcc aattgttttt
      961 aatatccata acgacatttt tcgtaaacgt tgatccgatt tgcacaccta atagtacaat
     1021 acctaattca ctaagtaaga atggccattt aatatcaagt ttgaaaactt ttacacaaat
     1081 gattgatgcg ataataggac caaacataaa tggaagtaat acgtgcgatg aatacagtag
     1141 aatactaata aaaaatgata aaacgaacac aatgaaatta tttctatata tcattgccat
     1201 gttttccact tctttcaata aaaaataaaa tgactaaatt gctgcttgag cttcacgttt
     1261 gttaagataa caatatccgc tagcagtttt gactacaaag catatatgga ctttcactat
     1321 caagtcgccg cccatgcctt atatacattt aaaaagagcc tgaacaaagt tcaggctctc
     1381 aatttgtccg tatatttatt ttacaatacg acttaaagcc gtatcaaatg cttgaatcgt
     1441 tttttcaata tcttctttcg tgtgtgccgt agataagaat gtaccttcaa attgagatgg
     1501 tggtaaaaac acaccttctt ttgccatttc tcgatacatt tctgcaaata atttcaaatc
     1561 acttttattc gcttgttcaa aattagttac aggtccttca tttaagaaat aaccaatcat
     1621 tgaacctgct ctatttacag ttattggtac attgtgttta gcaaatacac gttttaaacc
     1681 gtcttcaagt atatcgccta acatattaaa atactcatat gtctctggcg ttaattggct
     1741 taacgtttca taaccacttg tcattgcaag aggatttcct gataacgtac ccgcttgata
     1801 aatatttcct aatggtgcta tatgatccat gatttctttt ttaccaccaa atgcacctac
     1861 aggtagtcct ccaccgataa cttttcctaa gcaagttaaa tctggtgtca caccaaagta
     1921 accttgtgca caatgataac cgactctgaa accagtcatt acttcatcga aaattagcaa
     1981 tgcgccgtat tcagtcgtaa tatctcttaa tccctgtaaa aaaccttcaa tcggcggtac
     2041 gacacccata ttaccagcaa caggttctac gattacacca gcaatatcgt ttccaaattt
     2101 ttcgaaagcg attttaagtg catctaaatc attgtatgga actgtaattg tatttttagc
     2161 aataccttca ggcacaccag gagaatccgg caatcctaat gttgccaccc cagaaccagc
     2221 tttgattaat aacgaatcac tatgaccatg atagcaacct tcaaatttca caattttatt
     2281 tctgccagta taaccacgtg ctaatcttaa agtatccaat gtagcttctg taccagatga
     2341 caccatacgc actttttcta ttgaaggtac tcggtcaata acgagctgcg ccaatttatt
     2401 ttcaagtaat gttgatgcac caaaacttgt acctttatca attgcttcat gtaaatgact
     2461 aataacttga gggtctctat gtcctaaaat aagtggtccc caacttagta catagtcgat
     2521 atactcgtta ccatcgatat cataaatttt tgaaccttta ccgtgatcca taaaaattgc
     2581 tggtgtatct actgatttaa atgcgcgtac tggactattt acaccaccag gcattaaagt
     2641 ttcagcaacc ttcattgctt cttctgattt cgtatatctc ataaagtaaa ttcctcctac
     2701 agtttatgga aaatcataaa tataaaacct tatttatcta aatagcgaca aatgtccttt
     2761 gcaaaatacg taataatcat atcagcacct gcacgtttca ttgaaaccat ttgttccata
     2821 acgacacgtt cttcatctat ccaaccattt tgtgccgctg ctttagtcat actatattct
     2881 ccactcacat tatatgcaac aactggaaca ttcgtatgat ttttaacatc tcgaactata
     2941 tctaaataac ttagagcagg tttaacaatc atcatgtcgc acccttcttt aagatcactt
     3001 tctaattcac gaagtgcttc caaacggtta gcagggtcca tctgatacgt ttttctatcc
     3061 ccaaatgatg gcgctgaatc tgctgcatct ctaaaaggtc caaagaaact tgatgcatac
     3121 ttgacaccat aactcattat aggaatattg taatagccgg cttcatctaa tccacgacga
     3181 atttcagcaa caaaaccatc catcatatta cttggcgcaa taatatcagc accagcttcc
     3241 acttgagaaa ttgctgtttt aacaagtagt ggcaatgatt tatcattgtc aacgtcatgt
     3301 gtatggtcat caatcacgcc acaatgacca tgatcagtat attcacataa acaagtgtct
     3361 gcaacaatta ataagtcatc atacattttt ttagcaatac gtgttgcctg ttgaataaca
     3421 ccatcgtgaa tgtatgcacc agtacctata tcatcttttg agtttggaac accgaaaaac
     3481 ataatggcac gtatgcctaa gtcataagct tcttttaatt cactttcaag taaattcaaa
     3541 ctgatttggt atacacctgg caatgactta atttcttttt tcacatcgtc tttttcaact
     3601 acaaaaattg gatatattaa atcttctttt cttacatgat tctctctaac catatctctc
     3661 attgtcgctg atgatctcaa tcttctatgt ctatcaaatt tcatttttag cccctacttt
     3721 ctaaaatctt ttcaattagt gattcgagtg tttgaatttc tgcaattgtt actggttgtt
     3781 gatatgattt aatggtccgt gctgtttgtt ctccaatagc aaaatacgac ttgaattttg
     3841 gtacaaatcc ttcattaaaa taataacgta ctgccgacga acttgaaaat gttaatgcat
     3901 cgatttgttg atgttctatc atttctttaa catcttgtat attttgtttg ttaggcactg
     3961 aagtatataa atctatttta acaacttcat tatctttaga taacgctgct aataacaatg
     4021 gtctcgccaa ttcactcgaa ggcaaaagta ttttttggtt agtttgatta aatgatttta
     4081 aaaatccttc ttgagaaaag tcgtttggca taaaatcaac tcgaatgcca agtgattcac
     4141 aatattgcgc tgtcttactt cctatcacag caatgttatc aacattaatt ccttttagat
     4201 atttataaaa gaatttcaca gcatttttag atgaaaaaat aagccagtca tagcgttgat
     4261 ttaacaaatg aatatcaaaa tttagtggct ttatatcaat aaagggtttg tgaataattg
     4321 atactaaatc actttgcatg tcatttgttt gtgtcataac tacaactggc ttcatattta
     4381 aacgtcactc cattatttaa tgttgttcat ttaagcgttt tataatttca taagcacctt
     4441 gctcttttaa tttgttactc actgttttgc ctaactcaac cggatctgtt ccgttcattg
     4501 tatattcaaa tcgttcttta ccatctgggg tcataattaa acctgtaaat tcgatttcgt
     4561 tttgatctga gattgtagca tatcctgcaa ttggcacctg acaactacca tccatttctg
     4621 ctaaaaacgt tcgttcagca gtcacacatt ttgcaacctc atcattatgt actttgctta
     4681 ataatgttaa tagttcttca tcgtcactac gacattctat ccctaaagca ccttgtccga
     4741 ttgcaggtaa caatgtatct ctatcaagat aagatgttac aatatcatct gaccagccca
     4801 ttcttcttaa accagctgca gctaaaataa tcgcatcata atcttcagtt tgtaactttt
     4861 ctaatcgtgt atctatatta cctctaatcc atttaatctc taaattagga tacttagata
     4921 atatttgtgc accacgacgt aatgaactag taccaataat actgccttct ggcaattggg
     4981 atagtggtgt atgtgtttta gaaatatacg catcaaaagg taattctcta tcagggatac
     5041 aacctaatgt taaaccttcc ggaattacac ttggtacgtc tttaagcgag tgtattgcca
     5101 tatcgatatt tttttcaaaa agttcatgtt gtatttcttt aacaaataag cctttgcctc
     5161 cgactttaga caattgttta tctactatac gatcgccttt cgtgacaatt tctttaattt
     5221 caatttctag atttggctcg acagctttta atttatcaat aaattgctgg ctttgtgtta
     5281 aagctaattt acttcttctg gagccaacga ctaatttacg catgttcaat tcctcctagg
     5341 aacggattgc tctagattat tttctcaatt cacaaaatgt gttgcaaaaa ataaattaat
     5401 catatttaag caaaataaaa taatgttata gtatattaaa tatcttgaat tcaaccattt
     5461 gttgattcta agtaaaatat aacttccata taatactgta ataattgaag agagtattac
     5521 cttcgggtca atgaatatac gttcaccaac tgaaattaca ccccactgtg tacctaaaat
     5581 aatactaaat atgagaatta tccacccact taacgttgag taaaacacaa ttgattcaag
     5641 tgtagcaacg ctaccaattc taaagtattt ttgatcaaaa cgtttttcct tcaaattacg
     5701 gtattgcatg atatacagta atgcattgac aaaagctaag gcaaagaaga cataacttaa
     5761 cacagctaga ccgatatgga ctaacagtaa ctcgtctaca acagcaattt tctgaacctt
     5821 attagtataa tgtgtcggtt gaaatgtatt catccctaaa agtgttaacc ctattaaatt
     5881 caagaaaaac acagagaaat tcaaaacttt gataaggtta agaattaaag agattgaaat
     5941 aatcagccaa cttaaagtat agaatacatc tgaaatagac cctaatggaa tatgtctagt
     6001 ttgtataata aaaatagata aagagattgt ttgtaaaacc caaacaatcc ccaataaata
     6061 tatgcctaaa cttctaatct tatgactttt ttgtacaaaa tcataaaaat agcaaatgat
     6121 actgattaag tatattaata atataatttc attgaatcga ataaacaggt tttcttgcat
     6181 attatcacca tattaatatg cttattcaaa actaaagata cgtcgcgctg aaatttcctt
     6241 gaccttgctt tctttttgtt gcttcgcttg ctcatgagga cattcagctt cgatatcaaa
     6301 tatattttga aatagctcta atttttcatt acttttctta tcactactta attctttggc
     6361 ctgtttaata ggatctttca acatttgatt gatgatactt tttgtatgtt tagatataat
     6421 tttacgttct ctttcgctta accctggcaa tttacgatca atactgtcca tcgtttctgc
     6481 ttgaattgcc atagcttttt cacgtaaagc tctaatcact ggaacaacac ccaacatact
     6541 aatccactca ttgtgtgcat gtatttctgc aggaatttgt tccgaaattg ttgcagccgc
     6601 taattgtcgc tcacgtaagt ttgcatcaac taaacctttt aagtcatcaa catcataatt
     6661 aaagatgttt gtgatggcac taataccagg ttcaatatct cgaggaactg caatatcaat
     6721 caatactagt gaatcttgct ttctattttc tgcaattctt tctatcattt catttgtaat
     6781 gatataagat tgtgcactcg ttgaactaat cacaatatct gcactttcaa gtaaatttgg
     6841 taatgatgat agttcatcat atttcacttg atgctttgct gctaatttca tagcattttc
     6901 aattgttcta tttactactg taatatcagt aattccagaa ccaagaagat ttaatagtga
     6961 taattcactc atttcccctg caccaataat gatagcttgc ttacttttca atttgccaaa
     7021 tacttttttc gccaactcga ccgcagcata agacacactt acagcattat cagctatatc
     7081 tgtttcatta tgtgctcttt ttgcaaaagt aattgcctgt ttaaatagat gattaaaaat
     7141 tgttcctgtc gtacctgtgc tttgcgctaa gaaaaatgca tctcttattt gacctaaaat
     7201 ttgagtttct ccaagtacga ttgaatctaa accagaagtg acacgcaata aatgttctac
     7261 tgcttcgtcc cccactttta cttctgacat tgctttaata tcatctactt caaatccaaa
     7321 tgcacgagct agaaatcgtt gaatatagta acgacctgtg tgaatttgat caacaacagc
     7381 atatacttca gttcgattac atgttgataa tatgacattt tctaaaatag atttagtttc
     7441 atataaatct tcatgggcaa ttcgtaaggc atcatctcta aaagcaactt gctctcttag
     7501 tgcaacatca gctgtgcgat gatttatact aattgcaata aaatgcattt gaaacgcccc
     7561 catatatact aacaatacaa atattataac ataatttaaa atccaattaa gtacgatgct
     7621 taaataaatt ataatctttc taatttactt ttgagttttt gacaaaatga tgaacgttga
     7681 aaaatgttat caattgtgta ctaattaaaa gtttcgtgct tacattattt ttaaaatttt
     7741 tgtataagtt ttgacgtaca aaaactatga aatatacggt tcaattaaat tccatatttg
     7801 ttgttgttta ttattttgaa ttgatgaata acttacaatt gtatcgtctg ggtccatatc
     7861 taattgtgtc ttaatatttt taatatgctt ttgaacctta ccttttggaa ttttgtcttc
     7921 tttagtgcat ataactaaag taggaatatc aaaatgtttc aaataattgt acattaagat
     7981 atcatcttgt gttggatcat gtcttaaatc aactaattga ataactaatt gcaaattctc
     8041 tctcttagtt atatattcct caatcatttt cccaaatttt tcacgttgtg ttttacttac
     8101 tttagcatat ccataccctg gaacatccac aaaaataagt tgttcatcta tattataaaa
     8161 atttaacgtt tgcgttttgc cgggttgctg tgatgtacgt gccatatttt ttctgccaat
     8221 catactattg ataaatgtag acttacctac attagatcga ccgctcagtg caacttcaga
     8281 caattctgtt tctggatatt gttcttcttt tactgcacta atgattaatt ctatattatt
     8341 aggattaact ttcattttat atcctcactt ttttatattc tctttcaatt ctaccattat
     8401 ataatatttg aacctttcgg tatacattat aaaaaagctc cgatcaaagt taaactattt
     8461 gagcgtcaac tttgattgga gcttttcact tttataacac atcaatgatt aagctgatgt
     8521 tttactatta ttaattaaat tgccttctgc gtcgtatagt tctggttcag tttcttcatt
     8581 aattgtttgt gctgtaataa ctaccttcgt tacattttcg ttagaaggca catcaaacat
     8641 aatatcgatt aacgattctt ctatgattga acgtaaacca cgcgcacctg tttttctttc
     8701 aattgctttt tcactaattg ctgataaagc ttcttcagtg aactctaaat ccacatcatc
     8761 taattccagc attttagtat attgtttcac aagtgcattt ttaggttgcg ttaagatgtt
     8821 tttcaacgca gttacatcta atgtttctaa attagctaca attggcacac gtccgataaa
     8881 ttcaggaatc aaaccatagg cttgcaaatc ttctgggcga atttgtgcta ataatgcttg
     8941 ttcgtcatat ttatcagctt cattgcttga gaaaccaata actttttcac caagacggcg
     9001 cttaatcact tcttcaatac catcaaaggc accaccaaga ataaataaga tatttgttgt
     9061 atcaatttga atcatttctt ggtttggatg tttgcgtcca ccttgtggcg gaacacttgc
     9121 agtcgtacct tctaagattt taagcaatgc ttgttgaaca ccttcacctg aaacgtcacg
     9181 tgttatagat gtgttttcag atttacgtgc aattttatca atttcatcta cataaataat
     9241 acctttttcg gctttatcaa tgtcaaagtc agctgcttga attaatctca acaagatatt
     9301 ttcaacatca tcgcctacat aaccagcttc agttaaactt gtcgcatctg caattgcaaa
     9361 tggtacattc aacgtcttgg ctaaggtttg agctaataat gttttaccac tacctgttgg
     9421 cccaattaat gcaatgttac ttttttgtaa ttcaacatca tcttcttttg gtcctaattg
     9481 ttgaatacgc ttatagtggt tataaacagc tacagctaaa gattttttag ctttttcttg
     9541 accaataaca tattcgttta aatgatccat aatttcttta ggagtaggta attctgtcat
     9601 cgcttcagaa gtgttttgag ctaattcttc ttcgacgatt tctgagcata attcaataca
     9661 ctcattacaa atatatacac cacttcctgc tacaagtttt tttacttgat cttggtcttt
     9721 tccgcagaaa gagcatttca aattttcttc atcttcattg aatttaaaca ttctttttac
     9781 acccctattc gttaaagact atactagatt ggatgttaca atgcaacata ttaacataca
     9841 aactttttgc ttaaagaata gtagcagata cataagctat gcatctgcta ctcatttttg
     9901 taatttaata tttaatgaag attaatcttc tttagttcct tcaacgaact ttgcgttatc
     9961 tcttaataaa tcgataactt tttggatacg aacatcattt ttaatgatat cagtattacc
    10021 taaagtattt ttgatatctt caactgagat attaaattgt ttactcattt tttctaattc
    10081 tttatcgata tcttcatcag tagcttcgat tttttcagct tcagcgatcg cagttaaagt
    10141 taagttagtt ttaacacgtt gttctgcatc gtctttcatt tgctctctta attgagtttc
    10201 atcttgacct gagatttgga agtacgtttg taaatctaaa ccttgttgtt gaattctttg
    10261 tgcaaattca gacaccatac gatctaattc agtattaacc attgcttcag gaatatcgat
    10321 tgttgtatta tcagtagctt ttgtaatcgc ttcttctttt tcaacatttt cagcatctgt
    10381 agctttttgt tcagctaaac gtttacgtaa gttttctttg tactcgtcta ctgtatttgc
    10441 ttctgcatct aattcattag caatttcatc tgttaattct gggacttctt taaatttaat
    10501 ttcgttaact tttgttttga aagttgcttc tttaccggct aattcttcag catggtattc
    10561 ttctgggaat gttacgacaa catctttttc ttcgtcaact ttcatacctt ctaattgctc
    10621 ttcgaaacca ggtatgaatg aacctgaacc gatttctaaa tcgtaacctt cagcttgtcc
    10681 accttcgaat tcttctccgt caactgaacc actaaagtcg atgttaactg tgtcgccatt
    10741 ttcaacaaca ccatcttctt taacgaccat ttcagctaaa tgtcctaagc tgtggtcaat
    10801 cgcttcttgt aactcatcat cagataattc agtttcttgt ttttcaattt caagaccttt
    10861 atagtctcct aatttaactt ctggctcaac tgtaactgtt gcttcaaaaa tgaaatcttt
    10921 acctttttca atttgagtaa cacttacttc tggttgtgca actggtttaa tatcagtttc
    10981 gtcaattgct tcaccataag catctggtaa taaaatgtcg atagcatctt gatataatgc
    11041 ttctacacca aagcgttgtt caaaaattgg acgtggcact ttacctttac ggaatccagg
    11101 tacgttaatt tgtttaacca cttttttgaa tgcttgatct aacgctttgt ttactttttc
    11161 tgcaggaaca gtaacagtta ataaaccttc gttaccttcc tttttttccc aagttgctgt
    11221 catgtatata tacctccatg attaactaat ttattttttc aacttcccta ttatatcata
    11281 ggtctattcc ctatacaaac attgaaatca caacgtttat atatttgtaa atcaactttt
    11341 ttcgtcaaaa ctatattaaa atgctgagta ttattttgca acaatttaac tattattatt
    11401 caaatctaat tgctgaataa aattaaatgc atcgatactt tgtaattcac atttaagtcc
    11461 taacatattt ttgaaataac attcgtatgc attaatccat ttattcgttt caaacaaagt
    11521 ttcaatatca attggataaa tcatgataga gtgattattc ataatatggt gtgcttcttc
    11581 agcgatatgt aaagcaccat catttaatgt ttccataacg ttaggtataa ctttttcttt
    11641 aagtgttgta tgttctagcc cttttaaatt agctggtaca aaagttacat ccataccata
    11701 cttttcaatt gtcagttctt gtgtacaatt tgcgaacctt aaatactcaa tcattaaact
    11761 aatgaggtta taactgtacg tattagattt taatatatat aataccgttt cttgaaattg
    11821 aaaatgacca ttgtctatta acttcaaaat caagtgcgtc tgttccctca ttgataacgt
    11881 atcaaaatca gctaatgact gagtcaatcg tttttcatct tcaattaatt ttgacttagc
    11941 aaattcttta agaggatgta atgccattct tgttttgtga tctttgactt catcaataat
    12001 ttgatgaatt acttctaccg cttcaaaata ttgtcccaaa ccaatcaaac ttttcacgta
    12061 ataaatcatc aatgcatcat attgttgtat gccagttttc aataagacaa ttgtttcttc
    12121 tcttaattca agaaatgacc ccgtctcata taacatacgg catttcatca ttgctatttc
    12181 ttcagtgagt tcaaattgac gttcatattg cacaatatat tcatacatca ggtcatagtt
    12241 ttggtcttgt tcagcacgtt ttatatcttt atataacttt ttcgaagagt taggaaactg
    12301 aataatatct gacatatatg tcacctcgtt tgattataaa tcttgaagga ttaatgcact
    12361 ccacgtttcg aaatcatcat atgactctat gttgcctttt tcaacccatt ttattcgtaa
    12421 agtatctgtt tctttagctt ctacatcatt cgtacttaca gtgattttaa atacaacacc
    12481 aatatgtacc ttgcccactt cattattatc gtcattaata aaaccgatat attccatatt
    12541 ttgtgaatct tgctcactta aacctacttc ttcttctaat tctctctgtg cattaactct
    12601 caatacttcg ttaatagatt ctgctcctgg aacatcattc atatgaccgc ctacacctat
    12661 tgaagattgt ccatgcaatc gagcttctcc accgccagat aatcgttcat acactaatat
    12721 ctcgccatgc tcattttcaa gtaaacaata agaaatgagt tgtttatatg atggatcctc
    12781 ttccatatcg ccgcgtcgct taacttcata ttgacttagc gcatcaaaaa tattttgacc
    12841 ttctggttta ttcttattta aaaacccatt gaaagtattc ttttcattat taaaaataat
    12901 ttctctaggt actacaatga tttgttcatc aaatttagac atcgtataaa actcctcact
    12961 ttaatgtttg ttctcttaaa tgcattttag caaatatgtc atattttagc gattaaatgt
    13021 ggcattttgc tatttaaaaa ataaaaaaga gatgacaatg ccatctcttt tttataagtg
    13081 ttattatttt aaagcatctt tagctttagt tactaattga gcaaatgctt tttcgtcaga
    13141 aattgcgatt tctgataaca ttttacggtt aatgtcgata ccagcttttt tcaaaccgtt
    13201 cattaaacgt gagtagctca tttcatgttg acgagctgct gcgttgatac gtgtaatcca
    13261 taatttacgg aagtcacgtt tacgttgacg acggtcacgg aaagcatatt gacctgattt
    13321 cattacttgt tgcttagcta ctttgtataa tgtatgtttt gaaccgaagt aacctttagc
    13381 taatttaatc gtttttttac gacgcgctct tgttactgtt ccacctttaa ctcgtggcat
    13441 aaaaaattcc tccttaggta attcctaccg atttctgtat ttgttcttat tttttgtatg
    13501 ctaataattg ttttacacgt ttcatatcgc tcttagacac taatctagct ttacgtaatt
    13561 gacgtttttg tttagtgctc ttgtttgcga ataagtgaga tgtgaaagct cttgaacgtt
    13621 ttaattgacc tgaagcagtt cttttaacac gtttagctgc tccgcggtga gttttcattt
    13681 ttggcataat ataagtcctc ctgtaatagc tttaattatt tttcagctgt tggcgctaac
    13741 atgataaaca tttgacgccc gtccatttta ggtttttgtt caactgttgc tatatctttg
    13801 cattcatctg catatttttc tagcacacgt tgaccaattt ccttatgcgt aatggcacgc
    13861 cctctgaaac gaatagatac tttacattta tcgcctttag ttaagaattt acgtccgttt
    13921 ttcaacttcg tttggaaatc atgttcctca attgttggac ttaaacgaat ttctttaaca
    13981 ttgataattt tttgtttctt tttcatttct ttttcttttt tctgttgttc gaatttgaat
    14041 ttaccgtaat ccataattct tgcaactggt ggtttcgcat tcggtgcaac gaccactaag
    14101 tctaaatcta cacgttcagc catttctaaa gcttcacgct ttgatttaac accaatttgt
    14161 tcaccatctt gaccgattaa acgtaattct tttgcacgaa ttttgtcatt gatttgagtt
    14221 tgatcttttg ctatggttga cacctccaaa atttttacga aatttgcacc aagcaaaaag
    14281 gaagagcagg tataaaatac ccgctcttcc ttatacacag ttatgtgtaa tgtgattaac
    14341 ctgccaactg ctttatgcgt cgctacaggt gagaagcggg tgcttctact tggttcgttt
    14401 cgtattcaac gttattaatc atatcaacaa ttcacattta agtcaacact ataactgtaa
    14461 ttatttttat tttaaccttt tatttcatcc attgacacgt cttgacgtaa atctacttgt
    14521 tctaatggaa tttttttcgt tttatatcga agcttatgat aaataaagaa tgctaaaaat
    14581 actggaattc ccatatacgt aattaagaag cgactaaaat taaaatctcc tgttttaata
    14641 aagtcaacat cttgaccaat aattactaca atacataaaa agccagcaaa taatggtccg
    14701 aatgggaata atttagcagt atattttaat ttagatttgt cataattttg tttatcaaat
    14761 gctcttctaa atcgataatg acttactgct atacctaccc aagcaataaa accagttaaa
    14821 ccacttgctg caacgatata ttcgtatgca ccttttgtaa ggctttgtaa tacgaaaatt
    14881 atcactacaa tgattgctgt aacgagtaac gacatatatg gcacaccgtt tttatttgtt
    14941 ttaccaaatg cttcaaacgc taatttatct ttactcattg aatatagcat tcgagttgaa
    15001 gcatacatac ctgagttacc tgctgataac acagacgtta aaatgactgc attcataaat
    15061 gatgctgcaa acgcaaatcc agcattttta aacactaatg tgaatggaga cgttgctaca
    15121 ttatcactac cccccattaa tgcactacta tcataaggaa ttaacatacc gataacaaaa
    15181 atggctaaaa tgtaaaataa taaaattctc cagaatactt gtttaattgc tttcggcaca
    15241 gcacgttcag gattttctga ttcaccagcc gtaataccaa ttaactcagt accttggaat
    15301 gagaaaccag cgattaagaa tacacctaga attgataaca aacttcctcc taagttgcca
    15361 ccaagaattg gaccttcacc tttattaaat atttcgaatc ctacaacatg accacccatg
    15421 attccgacaa tcgttaataa accaattgca atgaaaacaa taactgtaac cacttttatc
    15481 aatgccaacc agtattcact ttcaccatag acgcgaactg ataacgaatt cagactaaaa
    15541 attataacta agaacaacgc actccatgcc caagcgggta tgccttgcaa tggtgtccaa
    15601 tattgaatga cttgtgctgc aatcgtaata tctgctgcta cagtcactac ccagttaaac
    15661 caatagttcc aaccaagcgc aaaccctaaa gatggatcaa caaatcttgt agcatatgta
    15721 ctaaatgaac ctgatactgg caaatacgta gccatttcgc caagtgacgt cattaagaaa
    15781 aataccatta ttccgataat tgcgtatcct attaatgcac ccaaagcacc tgcatcatga
    15841 attgctccac cagaagttac aaataaacct gtaccaatac aacccccaat cgcaatcata
    15901 gaaatatgac gatctttaag tccccttttg acaacattgt tactttcatt ttgaactttt
    15961 gacatataag ctccctttcc atgttttaga ggccaagaaa taaataacct ttattcactt
    16021 tcataccctt ttttacagaa catagtgata ttaaaagtaa aaaagtggct acataccttt
    16081 aagatatgca accactttgg caattatcta tataaggtag cacatcacat tctgtgacag
    16141 tacagttcct attcgaaaac tgtcccaata gatataattt atggtttata tctatttcgg
    16201 catctttacc tttcacttgt tcaacttatg taccataaat acttctgaca agttactaat
    16261 taaacatgca acctctaact caatttaata ttttaactaa cttgtaatat acaggattca
    16321 tcacgcataa tcaaccctgt aaaacttgat acgcaataaa agttttaaag cattttattg
    16381 cgacaactgt ctatctatgt tttttcaaac gaatttcatc aactagattc cagataaatt
    16441 catctttttc aactgtttct tggtcttgcg atccatattg acgcacattc acttgattat
    16501 tttcaacttc cttatcccca actacgattt gataaggtat tttttgcatt tgagcttctc
    16561 tgattttata acccattttt tcattacggt catcaatact tacacgaacg ccttgagatt
    16621 tcaattcatc ttgtaattgg cgcgcataat cataatgtaa atcaacgtta actggaatga
    16681 tttgaacttg ttttggcgct aaccaagttg ggaatgcacc tttagtttct tcagttaaaa
    16741 atgctacaaa tcgttccatt gttgatacaa caccacgatg aataacaact ggacgatgat
    16801 gttcaccatc ttgaccaata taagttaaat caaaacgttc tggtaataag aaatcaagtt
    16861 gtgctgttga taatgtctct tctttaccca tcgctgtttt aacttgaaca tctagtttcg
    16921 gaccatagaa tgccgcttca ccaatcgctt cttcgtacga taagccaagc tcatccgctg
    16981 cctctttaag catattttca gctttattcc acatatcatc atcatcaaag tacttttctt
    17041 tatcttcagg gtctctataa cttaatctaa agctataatc ctcgaaacca aagtctttat
    17101 acacatcaat aatcatgttt acaacgcgtt tgaattcttc tttaatttga tcaggtcgaa
    17161 caaagatatg tgaatcattt aaagtcatac cacgaacacg ttgtaatcct gatacagcac
    17221 cacttgcttc atatctatgc atcgttccta gctcagcgat acggataggt aattcacgat
    17281 atgaatgtgg tttattcgca taaatcatca tatgatgtgg acagttcatt ggacgtaata
    17341 ccatagattc agtttcatct aactgcattg gtgggaacat atcttcttga tagtgatccc
    17401 agtgaccaga tgttttgtat aaatcaacat tagcaagtac tggtgtataa acgtggtcat
    17461 atcccatgct aacttcttta tcaacaatgt aacgttcaat ttcacgtcta attgttgcac
    17521 cgttaggtaa ccataatggc aaaccagcac caactaattg gctatttgtg aatagttcta
    17581 actctttacc aattttacga tgatcacgtt ctttacgctc ttctaacatt tgtaaatgtg
    17641 ctttcaattc ttttttatca aagaaagcag taccgtatat acgttgtaac attttgttgt
    17701 tactatctcc acgccagtat gcacctgctg tagataatag tttaaactct ttaattttag
    17761 ctgttgatgg aacgtgaact ccacgacata aatcagtaaa atcaccttga ctatataatg
    17821 ttacattttc atcttcagga atcgcgtcga ttaattctaa tttgtattca tcattgctga
    17881 ataactcttt cgcttcatct cgtgaaacca cttttcgttc gattttcata ttttcgttaa
    17941 cgatttgttt cattgttttt tcaatttgtt caaagtcatc agatgagatg ttttggtcaa
    18001 tgtcgaagtc atagtagaat ccaccttcta ttacaggacc tacaccaaat ttaacattac
    18061 catataacct tttaatcgcg tgtgccatta aatgtgcagt agaatgacgt aatacctcta
    18121 acgcttcttc actacctggt gtcacaattt caattgatcc atcagtttca agcggtttag
    18181 ttaaatctac aagttgcccg ttaaatttgc cggcaacagc ttttttacgt aatccaggac
    18241 taattgattg tgctatatct tcagtagtag tacctttatc aaacgccttt ttattaccat
    18301 ctggaaattg aatattaatt tgttccatgt taaaccctcc tatttgcttt ataaatactt
    18361 ttatctgacc tcgttaaaaa attgaacaac aatcatcgtt ttaacattaa acctattaac
    18421 aataaacagg gaatttatga caccaacaaa catttatgtc tgaaaaaaac aaaaaatccc
    18481 gcccctatac aagggacgag atcgtcgtgg taccacccta gttatctaat acaatttatg
    18541 tattaaatat ctctgcttaa gataacggtc ttgatccggg tattcattac aaataccata
    18601 aatgaagtag taatcatcta atttattaac catattctca tcaaacaatg gctttctgtg
    18661 taatattgat tagatcatct tgtcttcaat catttaaacg atttagattt gtattcatta
    18721 tacaccaatc attttaaaat tcaattgttt ctgaaatttt ctcctgataa aaagtatggt
    18781 gttgacaaag atttgacacg ttcaataata cgtgctgctt tagtcttctc ttcaccatca
    18841 cgagtcatcg ctaaatgatg ttccaattca ctatagtcaa aattagaact aaagaatgtt
    18901 ggtaattcat gaaccattcg ataatgtagc aaaggtccaa ttacctcatc tctcacccat
    18961 ggagtcactt cttcagcccc aatatcatca agcattaaaa tgtttgcttc tcttacgcga
    19021 tgtaatttct tttcaaaaga accatcttta aagccacctt ttaatgttct aataaattcc
    19081 ggtaaataaa taattgtcga acgtaccttc ttagatttga gctgattcgc aattgcacct
    19141 agaataaaag atttacctgt cccaaatgga ccataaaggt aaaggccttt cacttgttcc
    19201 ccattagtta ttgctgtaca aatatcatct gctgccatag ctacatcaag acggtctcga
    19261 tgattcatat aaatatcttt caatttggca tttaaagtat ctcgttgcat atgatgagat
    19321 gtaattagct cagcttcaaa gcgttcttcg tcgtacttga ttttacatgg gcattgtaaa
    19381 tagcgtattt taattcggtt attatcaaca tataactcag gcacatgccc ctttacgaaa
    19441 tttggacaat cagcaaattt atgaccgtca taatgttttt gttgatcttt atactcttgt
    19501 aacacattta agtcttcatc aatcatagca ttcgttaatt cagctcgatg cgcttccaaa
    19561 aattgcttaa catctgggtc attgattact tcttttttta tcttttctat tcttttttca
    19621 aagtcctgcg acgtgttaat tatactttta aattgcttca ttattgactg tcctcctccc
    19681 attttttaga taatttatct agaaatgctt gtcgatcttg ctctaattgt tgatcatcta
    19741 cgctattatc tttagccgaa tcttcttcac taggtttatc tctattttct aaccatttag
    19801 gtgttttttc ttttgaaata cgattacgct gcccatagta tgaaccacgc ttttggtaat
    19861 ttccgctaga accctcattt ttaggttgat taactttttt agcgtaatta tatgcttctt
    19921 tagctgtctt aatacctttt ttcttccaat ttgatgctat ttccaaaata tacgctttag
    19981 gaagtttcat atcttctttt aacatgacaa attgcaacaa aatattaatg acgccaaaag
    20041 acattttttc acgttcaatt aattcttcaa ccattgtctt ttgcgatata gttggttctg
    20101 attcagacca agaagctaac atatcaattg gactcgtttg ttcaagtaac tcaaaccatt
    20161 catcactttg tggctttgga ttcacttctg aagatttgcc cgccgaagat gatgtagcag
    20221 gagatttcac ctgtaattta ggcatttgat tttcgtgttc cattaagtaa tacgagcgtg
    20281 cttgtttacg catttcttca aaggataact gttgtccact tgtaattgaa tttaaaataa
    20341 catgcttcat gccatctgct gttaaaccat ataaagtcgc gagttgtgta attaaacgct
    20401 ttgcatcttt ggtaacaatg tcttgactaa taaaatgttt acctaacatt tgtctcaaca
    20461 tttcaaagtc aaaagattca tttgataaat cgataccttg gtacggttca ttaatcggaa
    20521 tatcacttgt atcgatatct atttttgtag acggcacttt aaaaacatca gtaaattgtc
    20581 ttgttacctg tttaaattca ctcaaatcaa tttgttgata ctcaaagtat ttcttcaact
    20641 catgaaatcg acgatgctcg acttcactat ataaaaagat tgacaacatt ggatcattaa
    20701 aaaataaatg tgctgaaggc ggttgaatta attggtaaac aaattgtgtt tcttgttcat
    20761 catgtttgac aaacgccttt aacaatccaa tcgcttcaag taagtccatt tgttgtctaa
    20821 actctagtaa attaatttta agttcattca taaaaatata atgagaaaga atcaatgttt
    20881 cattatgact ttctttaacg aattgagtca taaaatgata taaacccact gcttgcgttc
    20941 caattagcgg tgtatacagt cgattcaata cctctaaatg attcgtattt aaatcaaagt
    21001 gttgcataac tttgaattga tcctttggtc ttaagccgaa ttcgaaggct tgtcgtccca
    21061 tttaagcatc actccgtttg ttttcgctta aaatcccttg catcgatgct aacaattgat
    21121 caacatcttt aaattcttta tagactgatg caaatctaac atatgaaact tgatcaacat
    21181 gcattaacaa gttcataacg tgttcaccta tatctcgtga agacacttcc gtatgacctt
    21241 catctcgtaa ttgccattca accttgttag ttatgtcttc aagttgttga tatctaactg
    21301 gtcgtttctc acaagaacgc acaagtccat taagtatctt ttctcttgaa aactgctctc
    21361 ttgtgccatc ttttttcaca actataagct gactaacttc gatatgttca aatgtagtga
    21421 aacgtgttcc acaattttca cattctcttc gtcttcgaat ggcatttaat tcatcggcat
    21481 gccttgaatc tacaacttta gattgtgtag aattacattt cgggcatttc attacatcac
    21541 cctctttatt ttgattatgc ctaattatac tataaatcta gagatgaaaa aagaatccct
    21601 caatttaatt catttaacca aataatgaaa caataaaaaa cattatatcg ttacttatta
    21661 agtaatttgc atgacaatat tattgtatta aaaataaaaa acctaactcc gaagtcagag
    21721 ttaggctata aattaattgt attaacttgc acttacagtt tcttttgatg tcaaaagtgc
    21781 tccaatttgc tcagcaacat ctacaactct atttgaataa ccccattcat tatcatacca
    21841 agcaataact tttactttat tccctgacat gaccattgtt gattttgcat caataatagc
    21901 tgaatttgga ttagtattaa aatcaacaga cactagtggt tgatgttcga cttctatgat
    21961 accttctaaa cctgcatttt caaaagcttg gtttacttct tctgcagtta cttctttttc
    22021 taaatcaaca actaaatcaa cgagcgatac attctttgtt ggtacacgta atgccatgcc
    22081 gtgtaattta ccttctaatt ctggtaatac ttcttttaaa gctttcgccg caccagtaga
    22141 agtaggaata atgctttcat tacatgaacg tgcacgtctt aaatctttat gtggattatc
    22201 aatatttttt tggtcatttg taatagcgtg aacagtagtc attaaaccat taactattcc
    22261 aaactgatta tttaaaactt ttgcaactgg accaatgcaa ttagtagtac atgaagcatt
    22321 actaaaaatg tcaaatgctt ctatatctaa ttggttatca tttacgcctt taactaccat
    22381 ttgaacatgt ccaccttttg aaggaccagt taacaaaact tttttggcac ctgctttaat
    22441 atgtgcgatg gctttatcac catgattaaa tttaccagtt gcatctatag caatatcgat
    22501 atctaattct ttccatggca agttttcagg attgcgatca gcaaccaatt taattttatg
    22561 atctccaact tgcaatccat tttcaatcgg ttcaactttt agattatatt ttccatgtgt
    22621 cgtatcgtaa ttgattaaat gtgcaattgt ttcgggtgga taactagcat ttatcgctac
    22681 tacatttaaa tttttatttt gtaatgcaat acgtaatacc attcttccaa ttctacccat
    22741 accattaatt gcaatattcg ttgacattaa aagcacccct tgatctttat gtgttatact
    22801 ttatggaatt agtatagcat attatatgaa atttgaaagc gttttccgtt gatttattta
    22861 tcttttttat aaaaaatagt cattacttac atacatcaac gcgtacgtaa agtaatgact
    22921 tatttatagt gtaatattaa tcttcttctc cgtaattcgg cttttcaata taaccttctt
    22981 cttctaacaa tctctcaagg ttttgtttta attcaagttt atcccctaaa ttatcgataa
    23041 catgatcggc cattcggctt tttttatcaa tagaaatttg gctatagaca cgtgctttcg
    23101 catcttctaa tgacaaatta ttacgttgca ttaaacgatc catttgtata ctttcagaag
    23161 tgtatacaac ccacacttcg tctactgtat tttccaattc attttcaaat aataatggaa
    23221 tatccatgat tacattatat ccttgtttta aatattcttg cttttcttct tccataatat
    23281 ctcgcacgat aggatgtatg atagcattta attctaagcg tttttctgga tgattaaaca
    23341 ctagatcacc catataacga cgattcatct caccattttc atcaattgct tcatcaccaa
    23401 agacttctcg tacttgagct aaacctttac tccccttttt aacagcttcc ctggctgctt
    23461 tatcagcatc tactacttta aaaccgaata cggataagag ttctgatact gttgattttc
    23521 ctgaggcgat tccacctgtt agaccaataa ctttcggcat aatttcactc tttctttatt
    23581 tttgacatac tggacaataa tgactatttc ttgtcgcgat gatttttgtt tcaatttgac
    23641 ttccacacac tttgcatacc ggctgcttat atacattaag atgcaattgc atctcaccag
    23701 tttttccatc agcatgacga taatctgaaa tacttgtacc gccatattta ataccttctt
    23761 ctagtacttc tctaacataa taaaaaacca tttcttgttg ttggtgtgtt aagtctttta
    23821 cttttttatc tggtaaaaca cctgcacgaa acaacgcttc acatgcgtaa atatttccac
    23881 aacctgcgat tactttatga tccaaaatca cttgtttgat tggtttattc ttattagact
    23941 gttgatgaat tcgatttaaa taatacgtca atgcttcatt tgaaaaaggt tcaggcgcta
    24001 tttctaaaaa tgaaggataa gatgctacag acgcaacatt tctaatttct ccaaaacgac
    24061 gtatatctga ataaattaac tttttgtcat ttgacaactc aaaaataaca tgccaatgct
    24121 tacgataatt aggtatcata atatcttcaa gttcatctac aatgaaaaaa ccgcccgcca
    24181 tacctaaatg actaattaat gtacgttgtt ctcgtttatt atctagctga aaaacgatat
    24241 atttacttct tcgttctaca tttgtaatgg tatagccttc cgataaagtt ttaaaagtat
    24301 ctaattcaat tccttttata attgtttcct tgccttgagc tttaccttcg attactttat
    24361 ccgaaaatat aacgtgttca attttttgat ttataacgta gggttcaatt cctcttttta
    24421 catgttctac ttctggtaat tcgggcatac cattaacctc actttatttt gcatcatacc
    24481 aggttgcacc ataacttgag tctactttta atggaacatc taattgcaat gcattttcca
    24541 ttatctcttc tacaaattca ctaaatgaat ctacttctga cttaggtact tcaaaaatta
    24601 attcatcgtg tacttgtaat aatagtttag cttgatatgt tgtctctttc attttttgag
    24661 caaatttaac cattgccagt ttaatgatat ctgcagcact gccctgtatt ggcgtattca
    24721 tagcagtacg ttcagcaaag ccgcgtaaat taaagttacg actcgtaata tcaggaatat
    24781 agcgtcgacg atgtagcaat gtttccacgt aacctaaagc tttggcatct tttacaatat
    24841 cagacatata ttgttttaca cctgggaaac tagctaaata atcatcaatg aatgcttttg
    24901 cttttttacg agtaatacct aaactttgac ttaaaccata atcacttatc ccataaacaa
    24961 ttccaaagtt aactgctttt gcttgacgac gcattaaact atcgacttga tcagcttcta
    25021 caccaaatac tttcatagca gttgctgtat gaatatcatc gccgttgata aatgcttctt
    25081 tcatactctc atcttgtgta atgtgtgcta atacacgcaa ttcaatttga gaataatctg
    25141 ctgataatat aacgctatct tttgaagttg gtttaaaggc ttttcttatt ttacgccctt
    25201 cttcaagtct aaccggaata ttttgtaaat taggatctac acttgataaa cgtccagttt
    25261 gcgctaaagt ttgattaaaa cgtgtatgga tacgttgatc atcactaatt accttttgca
    25321 atccttcaac ataagtagac tgtaacttag ataattgacg atattctaaa atataatcaa
    25381 tgataggatg ttcaccttgc aattgctcta agacatctac agcagtagaa tatcctgttt
    25441 tcgtcttttt aataacaggt aattgtaatg tctcaaacaa tacaacacct aattgcttag
    25501 gagaatttat attaaaatct tcaccagctg catcatggat atttcgaatc aagacgtcta
    25561 atttttcttg aatttctttt tccatttctt ctaaatcatg aacatctgta aatataccaa
    25621 tttcttccat ttcacttaaa atcttagcta gcggtagctc taaatcagcc aagagttcta
    25681 cctgattgta ttcttctaat tgtttatcca tatttggttt cgcaaagtaa attgcatcag
    25741 taatagaagc aacatatgga tttaaaacat catcttcagg taccttaaat ttcttacctt
    25801 ttccatatat actcacatcg tctttcacaa aactttgacc gtacaatgaa acaactgatt
    25861 gaacatcact aatcgtacga gatggatcaa ttatataact ggccaacata atatcgaaag
    25921 aaatattttg aatatcaatc cccaatctat gtgatgctac atatgttttt ttagcatcat
    25981 atacgacttt tttcgaattc ggattttcta accatgaaac tagttcgaca taattattta
    26041 tgtcatccgc attaattaca atatgtttct cacctgtaaa taaagagaat tttaaaatat
    26101 tatttcgcaa atagttacca ccgtctaatt cgaaatggat ggccgcttct ttcaatgaag
    26161 taaaatcaat attatcaaaa gacgtttcaa tttcaaatgt cttttctatt gcatcttcaa
    26221 cgcttgctga ttgatcaatg tcagccaaca attgtttgaa ttctaacttc ttaaacaatt
    26281 cgattttttc ttgttgttca tcttgatgag tcattaacgt atcttcaagt tttacttcaa
    26341 tcggactatc tacattaatc gttgctaatt ctttactcat taatgcatct tctttgctat
    26401 tttgaagttt ttcttttaac tttttacctg aaatttcatc taaatgttca tagacacctt
    26461 ctactgtgtc aaattggttt agcaatttta ttgctgtttt ctctccaaca cctgcaacac
    26521 ctggtatatt atcagaagta tctcccatta atcctttcat atcaataatt tgattaggtg
    26581 ttaacccgtt gtatttttcc gcaataaagt caggtgtata atgatcaaca tcagtaacac
    26641 cttttttagt gtaataaatg gttacattat ccgttgcaag ttgtgttaaa tctcggtctc
    26701 ccgtaataat aattgtctga aagcccgctt tatctgcttc tttacttaaa gttccgataa
    26761 tatcatctgc ctcatagtta tctaattcat aacgtttaat atgataagca tctaataatt
    26821 ggcgaatata aggaaattgc tcacttagtt caggcggcgt tttctggcgt ccacctttat
    26881 attcactata tttttcatgt ctgaaagtcg ttttacctgc atcaaacgct actaaaaaat
    26941 gatttggctt ttcttctttt aaaatcttct ctagtaacat tgcaaaacca tatactgcat
    27001 tggtatgaat gcctgctttg tttgataaca aaggtaatgc ataaaaagct ctaaaactta
    27061 agctattacc atcgattaat actaatttat tcacaatttt aacctccaga actaatttat
    27121 atatatttta gcatattcga ctgtgatgtc gtaattaaag aaactatctc atagagtatt
    27181 tatacacgac ctaatttcaa ataaaacaag tgcctatata gacttagcta aaaaataaaa
    27241 aagacataat catgattgtt cttgatgagc actccataaa gtcaggttaa aagtccaact
    27301 tttgagatgc acattatcat ttcatgatta tgtctacttt ttaatatttt tatgctgcta
    27361 ttttaaattt ttataggtaa gaataagtta tgaatcgtct gaaccaaatg gaatatattt
    27421 aactttggtc attttcggta attcttcata attattaaac cactcttggt aatttttatt
    27481 gattggtgac ggatgatgca tataatgttc aaacggcaat gattccactg tataaacctc
    27541 ttgtcgtgga tgatctttaa tttgttgttt taataactga actctttttt catgttcata
    27601 atgaacataa ataaatgcac taagatatat cacagctaaa actagtgatg cacctttaat
    27661 aaaattaaca ttgattgact tatatttccg aaattctttt aataaaataa ttaaaatgat
    27721 tacatgtatc gtataaacaa tcaaaaaatt acctggttct attggagtaa caatgactag
    27781 tgtcgacgcc gaaacacata ttgcaataag taaggaatat aaagtgattt gtgttttacg
    27841 atcattaata gacaaataaa tacctacaaa tatcgaaaac gcaaagtaac cacatacaat
    27901 tacgttcac
//
LOCUS       DAMQYT010000014        26573 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMQYT010000014 DAMQYT010000000
VERSION     DAMQYT010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26573
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.014"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..1935
                     /gene="isdB"
                     /locus_tag="PGA33_000516"
     CDS             <1..1935
                     /gene="isdB"
                     /locus_tag="PGA33_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HDG6472355.1"
                     /translation="NKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEET
                     GGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEVK
                     APKETKEVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDGT
                     QQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVKD
                     YAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAEK
                     LLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQNV
                     QPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYWK
                     DFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVDK
                     EAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPSV
                     EKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVVQ
                     TSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLPL
                     MALLALSSIVAFVLPRKRKN"
     gene            complement(2089..2262)
                     /gene="rpmF"
                     /locus_tag="PGA33_000517"
     CDS             complement(2089..2262)
                     /gene="rpmF"
                     /locus_tag="PGA33_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDG6472356.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(2342..2899)
                     /locus_tag="PGA33_000518"
     CDS             complement(2342..2899)
                     /locus_tag="PGA33_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDG6472357.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            3026..4165
                     /locus_tag="PGA33_000519"
     CDS             3026..4165
                     /locus_tag="PGA33_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDG6472358.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTSNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            complement(4227..4709)
                     /gene="coaD"
                     /locus_tag="PGA33_000520"
     CDS             complement(4227..4709)
                     /gene="coaD"
                     /locus_tag="PGA33_000520"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDG6472359.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(4711..5253)
                     /gene="rsmD"
                     /locus_tag="PGA33_000521"
     CDS             complement(4711..5253)
                     /gene="rsmD"
                     /locus_tag="PGA33_000521"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDG6472360.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            5323..5712
                     /locus_tag="PGA33_000522"
     CDS             5323..5712
                     /locus_tag="PGA33_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472361.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            complement(5715..5969)
                     /locus_tag="PGA33_000523"
     CDS             complement(5715..5969)
                     /locus_tag="PGA33_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDG6472362.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            6208..7134
                     /locus_tag="PGA33_000524"
     CDS             6208..7134
                     /locus_tag="PGA33_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDG6472363.1"
                     /translation="MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     NLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            complement(7197..7631)
                     /locus_tag="PGA33_000525"
     CDS             complement(7197..7631)
                     /locus_tag="PGA33_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDG6472364.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(7647..8684)
                     /locus_tag="PGA33_000526"
     CDS             complement(7647..8684)
                     /locus_tag="PGA33_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDG6472365.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            complement(9011..9472)
                     /locus_tag="PGA33_000527"
     CDS             complement(9011..9472)
                     /locus_tag="PGA33_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HDG6472366.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            complement(9497..10408)
                     /locus_tag="PGA33_000528"
     CDS             complement(9497..10408)
                     /locus_tag="PGA33_000528"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDG6472367.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLFGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            10848..11759
                     /locus_tag="PGA33_000529"
     CDS             10848..11759
                     /locus_tag="PGA33_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HDG6472368.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            complement(11899..15351)
                     /locus_tag="PGA33_000530"
     CDS             complement(11899..15351)
                     /locus_tag="PGA33_000530"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDG6472369.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFNIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(15905..17131)
                     /gene="ftsW"
                     /locus_tag="PGA33_000531"
     CDS             complement(15905..17131)
                     /gene="ftsW"
                     /locus_tag="PGA33_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDG6472370.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            complement(17445..17720)
                     /locus_tag="PGA33_000532"
     CDS             complement(17445..17720)
                     /locus_tag="PGA33_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDG6472371.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            17861..18343
                     /locus_tag="PGA33_000533"
     CDS             17861..18343
                     /locus_tag="PGA33_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472372.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            18345..18512
                     /locus_tag="PGA33_000534"
     CDS             18345..18512
                     /locus_tag="PGA33_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HDG6472373.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(18652..20499)
                     /gene="typA"
                     /locus_tag="PGA33_000535"
     CDS             complement(18652..20499)
                     /gene="typA"
                     /locus_tag="PGA33_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDG6472374.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            20601..20792
                     /locus_tag="PGA33_000536"
     CDS             20601..20792
                     /locus_tag="PGA33_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HDG6472375.1"
                     /translation="MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(20946..21773)
                     /locus_tag="PGA33_000537"
     CDS             complement(20946..21773)
                     /locus_tag="PGA33_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HDG6472376.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWIVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPHHDALIQLHEQRFKRKSK"
     gene            21930..22544
                     /locus_tag="PGA33_000538"
     CDS             21930..22544
                     /locus_tag="PGA33_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HDG6472377.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLRELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATNKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLIEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            22728..24080
                     /locus_tag="PGA33_000539"
     CDS             22728..24080
                     /locus_tag="PGA33_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDG6472378.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQS
                     "
     gene            complement(24162..24581)
                     /locus_tag="PGA33_000540"
     CDS             complement(24162..24581)
                     /locus_tag="PGA33_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDG6472379.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWVIVMIMLISKKESKETTHKDDEYIYH"
     gene            complement(24864..25883)
                     /locus_tag="PGA33_000541"
     CDS             complement(24864..25883)
                     /locus_tag="PGA33_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDG6472380.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            complement(25957..>26573)
                     /locus_tag="PGA33_000542"
     CDS             complement(25957..>26573)
                     /locus_tag="PGA33_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDG6472381.1"
                     /translation="SFDSWKSLYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSH
                     HLKEAERDLTKLAPQVRGVVGDEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIP
                     KEGSNLWFDNMVIPKTAQNKEGAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPK
                     EIKDDHRFYPTKKEQERLEVYKDLGPEVLSEYNENFLNFKMSLK"
BASE COUNT         8989 a         4733 c         3799 g         9052 t
ORIGIN      
        1 aacaaacagc aaaaagaatt taaatcattt tattcaatta gaaagtcatc actaggcgtt
       61 gcatctgtag caattagtac acttttatta ttaatgtcaa atggcgaagc acaagcagca
      121 gctgaagaaa caggtggtac aaatacagaa gcacaaccaa aaactgaagc agttgcaagt
      181 ccaacaacaa catctgaaaa agctccagaa actaaaccag tagctaatgc tgtctcagta
      241 tctaataaag aagttgaggc ccctacttct gaaacaaaag aagctaaaga agttaaagaa
      301 gttaaagccc ctaaggaaac aaaagaagtt aaaccagcag caaaagccac taacaataca
      361 tatcctattt tgaatcagga acttagagaa gcgattaaaa accctgcaat aaaagacaaa
      421 gatcatagcg caccaaactc tcgtccaatt gattttgaaa tgaaaaagaa agatggaact
      481 caacagtttt atcattatgc aagttctgtt aaacctgcta gagttatttt cactgattca
      541 aaaccagaaa ttgaattagg attacaatca ggtcaatttt ggagaaaatt tgaagtttat
      601 gaaggtgaca aaaagttgcc aattaaatta gtatcatacg atactgttaa agattatgct
      661 tacattcgct tctctgtatc aaacggaaca aaagctgtta aaattgttag ttcaacacac
      721 ttcaataaca aagaagaaaa atacgattac acattaatgg aattcgcaca accaatttat
      781 aacagtgcag ataaattcaa aactgaagaa gattataaag ctgaaaaatt attagcgcca
      841 tataaaaaag cgaaaacact agaaagacaa gtttatgaat taaataaaat tcaagataaa
      901 cttcctgaaa aattaaaggc tgagtacaag aagaaattag aggatacaaa gaaagcttta
      961 gatgagcaag tgaaatcagc tattactgaa ttccaaaatg tacaaccaac aaatgaaaaa
     1021 atgactgatt tacaagatac aaaatatgtt gtttatgaaa gtgttgagaa taacgaatct
     1081 atgatggata cttttgttaa acaccctatt aaaacaggta tgcttaacgg caaaaaatat
     1141 atggtcatgg aaactactaa tgacgattac tggaaagatt tcatggttga aggtcaacgt
     1201 gttagaacta taagcaaaga tgctaaaaat aatactagaa caattatttt cccatatgtt
     1261 gaaggtaaaa ctctatatga tgctatcgtt aaagttcacg taaaaacgat tgattatgat
     1321 ggacaatacc atgtcagaat cgttgataaa gaagcattta caaaagccaa taccgataaa
     1381 tctaacaaaa aagaacaaca agataactca gctaagaagg aagctactcc agctacgcct
     1441 agcaaaccaa caccatcacc tgttgaaaaa gaatcacaaa aacaagacag ccaaaaagat
     1501 gacaataaac aattaccaag tgttgaaaaa gaaaatgacg catctagtga gtcaggtaaa
     1561 gacaaaacgc ctgctacaaa accaactaaa ggtgaagtag aatcaagtag tacaactcca
     1621 actaaggtag tatctacgac tcaaaatgtt gcaaaaccaa caactgcttc atcaaaaaca
     1681 acaaaagatg ttgttcaaac ttcagcaggt tctagcgaag caaaagatag tgctccatta
     1741 caaaaagcaa acattaaaaa cacaaatgat ggacacactc aaagccaaaa caataaaaat
     1801 acacaagaaa ataaagcaaa atcattacca caaactggtg aagaatcaaa taaagatatg
     1861 acattaccat taatggcatt attagcttta agtagcatcg ttgcattcgt attacctaga
     1921 aaacgtaaaa actaataaat cgtctttata tttaattatt aaattaacaa attttaattg
     1981 gcggatgagg tatccagtta cctcgttcgc caattatttt tcgcaatata aaaagtccca
     2041 cttaaaacaa tcattttaag cgggactttt tatattgagt aactaaaatt atttagctgc
     2101 tacttcttcg ccattgtaag aaccacagtt tttacataca cggtgtgata atttgtattc
     2161 gccacagttt gggcattcag tcatacctgg tactgaaatt ttgaaatgcg tacgacgttt
     2221 gttttttcta gttttagaag ttcttctttt tggtactgcc atgatatatc ctccttagat
     2281 tataaacgaa aaatactaaa tgttagttta attaacaaca ttatatcatt aattaaacta
     2341 cttattgctc tttatcatat aattgttgta atttttgaag ccttggatca acttgtcgtg
     2401 attctgaatc atcttgttct tgctgtttag caagctcatc taattgatcc tcatcgatta
     2461 cttcccaacc attacctact gtcaacattt ggtcactttg ctctgaataa gctctcattg
     2521 gtttctcaat aataactata tcctcgacaa tatcctgaag attaaccata ccatctttaa
     2581 taatgtgata gtgttcatct acatcatctt gatcatcgtt atactgattg tacccttcta
     2641 aatcaaatac ttctgtagta gttacatcta gtgggacttt tactggtaca agagtacgtg
     2701 cacaaggcat tgtatacgtt ccagtaatgt gaatatccgc aacgacttct gttgacttaa
     2761 tggttaactg accttggatt gtaattggag ataaatcaat taaatctaat gattctttta
     2821 aattgtcaaa actcaccgtt tgatcaaatt caaatggctt accttgatat ttccttaatt
     2881 gcgtaattga ccatttcata tggcttcacc tctaacaagc acaaaattta ttttaacttt
     2941 ctaagaacaa gttgtcaaga ttttttctta acatatcatg ttctgttaca atattaataa
     3001 tgaataccga aaggacgaat tgcaaatgaa aagcgttggc ttaatcacag aatataatcc
     3061 ctttcataat gggcatcaat atcatattaa tcaatctaaa aaacttacaa atgctgacgt
     3121 tactattgca ataatgagtg gtaactttgt catgcgtggc gaaccagcaa tctataataa
     3181 gtttactcgt gcaaaaatgg cattatcaac agctgattta gttatcgaac taccagcaac
     3241 tgccagttta tcatctggcg atcattttgc cgaactagca gttaaagtcg cagattatat
     3301 gagtgtcgat acaattgcat ttggtagtga aaataatgat atcaaaacat taaagcaatt
     3361 agcacacagc attaatgaaa ttgaacaatc tgaatccttt tcacaaaaag taaaagaagg
     3421 taaaagctac cctcgtatca ttagcgaatt attagagcat catgaagcac tagcaagtcc
     3481 taacaacata cttggtatta gttacctgaa agcaattgct aaaaatgcta aaaacatcaa
     3541 tgcaatttct atcaaacgag aaaatgctca acatcatgat tcattaattc aacaccatca
     3601 gtttgcaagt ggtacatcta ttagaacatc aatcattagt caagatgatc attggcatca
     3661 tgtggtacct aaagatattc aacacttgta cgtaacacct catataacat taaatcaaat
     3721 atttccatat ttgaaatatc aaatcatagc tatgactaca gattcattaa aaaatattta
     3781 tactgttact gaaggttttg aaaatcgttt gaaatctaac atttatgaag caactgattt
     3841 tcatcatttt gtaaaattac taaaaacaaa gcgttataca tatacgcata tccaaagact
     3901 gttaatgaat gtattattaa acattaaacc tacagatgtt acgagtaaca ttcacgcagt
     3961 taaagtatta gcgatgaacg accgtggcag acaatattta aagcatttaa aaacagcgtt
     4021 cccagaaaga caatatataa ccaatattaa caaaagcaat gcacattact tcacaaatga
     4081 aatcaaagca acacacatat acaatgcaat aagcgggcaa caacaaacag atttcaacac
     4141 gcctgtcata cagcaatatc gttaacaaat gaaaacagta ttttaggatt gtaaaccatg
     4201 ataaacctaa aatactgtta tttttattac ttaaatttct tcttcaatgc cttttcaaca
     4261 taaggtggaa cgaattcaga aatatctgct cgataagctg caacttcttt aacaatactt
     4321 gaacttataa atgaataatt agtactagac atcatatata acgtttcaat ttcattgttc
     4381 aactttttat tcattgaagt taagcgtaat tcatattcaa aatcactgac tgctcttaaa
     4441 ccacgtatga ttgttttagc tcctacttgt tcacaataat cgactagtaa accactaaat
     4501 tgatgaacct tgacattagg taaatgttta acagattgtt caattaaatc catacgctct
     4561 tctaaactaa acgtaccttc ttttttacta tttttaagaa cacagacatg aatttcatca
     4621 aatctatctg tacttctctc aataatgtct aaatgaccat aagtaatggg gtcaaaacta
     4681 cccggaatga ccgctattgt atgttccatg ctattctccc ttttctaata acaatgtgtc
     4741 tgtcaaccca taatggtaac gtttaatcat attaaacggt tgataatcta tttcttcatg
     4801 attgctaaat tcacaaacga tgataccatt ttctttcaat aaattaaact ctgaaattag
     4861 ttttaaagct ttatcaatga gacctttatt ataaggtgga tctaagaaaa tgacatcaaa
     4921 ttgaatatca cgttttgaca atgcttttaa agctctatct gcattatttt tataaacttc
     4981 agattgtgcc tctaaatcca aattcgcaag atttgattta ataactttta cagctttaaa
     5041 attttgatca acaaagatta ccttatccat acctcgagag agtgcttcta ttccaagcgc
     5101 cccgcttcct gcaaataaat ctaaacctat acctgacaca tcatataaac tattaaagat
     5161 accttcttta actttatcca tagttggtct cgtattacgg ccttccatac tttctaaagc
     5221 tttactttta tgtttacctg caatgacgcg catgttgttc acacttccaa ttcatttagt
     5281 tatttaatat aatttattga gaaaaaggag aatgataaac caatgaaaca aacatttatt
     5341 acacttggtg aaggtctaac agatttgttc gaattcatga cgatgattga atataaccat
     5401 caacgtattg ataaaattat ctattttcat tcaccacaag ctgaaaataa aaagtcatct
     5461 gtagcaatca ttatgaaccc tacaactggc aatcatttcc aagcatttta tatcatgata
     5521 aacgctatta aatatccata tccagattca aataaaaagt ttcaaatgat aaatgattgt
     5581 gctgaaaaat tcgacatacc aattttaggt atcgatgtac agccccctca agcatttcat
     5641 gatttatcgt tatattataa ttatttaatt agtgtgttaa ggctccaaaa atggatacca
     5701 gaacttcaat aatattaatt atatatttcg tgtttctctt tttcgtaagt tttctttaag
     5761 tatttatatg gtgagccatc aatgtgtcta acgtatttaa gttgcattag tttatgtaca
     5821 atttgatcaa catcttgctc attcacatac ataattacaa atttacgatc tctatttgaa
     5881 tgaacgatat gtccatattt tcggatttgt cgttcatgtt tcatatgttt taaataaact
     5941 acaatactag ttcttgggat taaattcatt taaacctcac tccatttatt ttcagcatta
     6001 atcatatcat gtctttatta attttaaaat attaagctac cttaattatg tcttatgtaa
     6061 ttgctcgata aatgtattta gattatatta aacatcatta tattaattca attctcaaga
     6121 gcatcactat atgattaacc acacatttcg ctgtagtcag aactatgaat ttgttaagct
     6181 ataaaagtaa acgaggagga attttttatg aaaaaagtaa acagaaacat tttaaatgcg
     6241 gtactagtcg gtgctggatt tttaagtagt ctattaatga tcaataaaaa taaagtgatt
     6301 actaaaaagc aaaccatccc tgcttttttc aaaggcaatg caccatatat atttgcgcat
     6361 cgcggtggta tggctttaag accagagcaa acgcaacttg catttgacta tgctaaacaa
     6421 ttaggcgttg atggctttga aacagatgtg agactcacaa aagatcaaca gcttatcgtt
     6481 tttcatgacg caactgttga tagaacgacg aacggctctg gaaaagttag tgcccataca
     6541 ttagcagaat taaagaaatt agacgcagct tatcacttca aagatatcaa tggactaaca
     6601 ccatatcgtg gtcatgcaca cacagctata ctaaccttcg atgagttgct gaagcaatat
     6661 cctgatatgt atataaacgt agacttaaaa gatgctcctg aatcctatga aggctcaatc
     6721 gcaccacaaa ttatgtttga tacaattgca gaaaatcaag catttgaccg cgttcttgtg
     6781 acaagttttt ataaagaaca aatcgttcgt tttaataaaa ttgcacaagg atccgtcgca
     6841 attggtgcta gccaacagga agttacagaa gcatttttaa aatatcattt attaggtggt
     6901 aggtactacc aaccattagc acaaacgttc caaatgccta ctcattttaa aggcattgat
     6961 ttgacttcat ctcgttttat caaatggtta aatgatatga atatcattcc aggctattat
     7021 ggcgtaaata gtataaatct gatgaatgat ttataccaaa aaggtgcaca cacgattgtg
     7081 acagatcgac cagatttagc acaacaattc aaacaaacaa tcccgaataa ataaaaacat
     7141 cgagtcagat gctagttagt ggtttcaata accacctttt tctgactcga tgttttttat
     7201 aatgaacaat tacaggatcc gcccgtcgca caaccactat gtgatgtttg aaagaatgga
     7261 ttacctgttt ctattttgac atgttctgaa acagcgtacg caattttcgt aacaacttca
     7321 tctattaaat gttgaagtgc catttcccta gctttgtaat gcatcacaac gtcaagcatc
     7381 tcatatgctc ttttgcgttg tcttgtctct aacataactt tcttataatc aggatgatat
     7441 ttcccgaatc gcattacttc atcatatttc tcttttgatt ttaaaaatga ttgataaagt
     7501 aaatgtgctt cgtcattgtt ttcgagcgcc tgtttagctt gctcaaatga tgcataaata
     7561 tctgatgcaa ctatcatatc agctagttca tcaatatcat ctaatatcgc tagtgaagct
     7621 tcattaatca taaatacccc tcattgttat ttttctaaga aaattagtga ataataatct
     7681 ctcattacat ctccacccat ctcatcgtat tttgaattta atagacgaga tcgatgtata
     7741 tctgaattca tccaactatg gattaatgtt ggtacatcat taaacgcata accaacattt
     7801 tgagcagttg ttttataagt aacgtgattt ttatctaatt gccctcttaa tgcgtcctct
     7861 gtaaattcaa cactatcaga accattagag gtcgcttcat ataagttatt agatgcaata
     7921 tgtgctaaat cgctattgat tttcaatggt tttaatcctt ttaattttct catttcattc
     7981 gttacttcat aaagagaaat taattgattt ggattttgct caactggacg cttattatgc
     8041 tcttctgacg tagaattaga atttaattga taaggttcaa tatctgctaa catttctttt
     8101 gttaaaaatc gtacacttag cacctttttc gattgttgat cagaatacac ttgtgcatat
     8161 atgtcgccat atttaatcag tgtttgtgtt tttaaatctt catctgaaag ttcaaattca
     8221 tattttttac catcaacttt aaaggacggt tctggattaa tacttgtatg attaaaaatt
     8281 tctgcagaat gttgtcctat ttttaacgga ctaacattga ctttctcacc tgtagcatac
     8341 actgaaacga tttcttcacg tttagttgaa acaatgtaat aactgttttt gtctttaaac
     8401 acataatttt tgtaaccatc tctaaaaggg tagacacgat ctgcttgtcc aaatttagaa
     8461 gtaagcactt taatatcttt acctacccat gtaccaacac cttctttagg ctttggattt
     8521 tcagcatggt tatttgattt attcacctgt tcagatctat ttactttatt acctttattc
     8581 gggttctcta atacatcaaa ttttaatctc ggtgaataaa aaagatatat taaaaatcca
     8641 actaaaaata atacaccgac gactcttata attaactttt tcatcaatca accacctaaa
     8701 aagtattaat actattgtaa aaaacaacac attaattagc aaattttcaa cactgacata
     8761 actgtgtcgt ttcgataaaa caaaatcatc ttcaggcata attttaacgt cataaagatt
     8821 ttccctacac tctatatcat aacctatctt tatgttttca ggttgaattt cattattaag
     8881 attaaaatat gtataaaaaa atggacagtt aaggtatcaa atgaatacca tcaactgtcc
     8941 gactattctt catcaaaaaa cctgataaaa caaattgcct tatcagatta gtatcatttg
     9001 tataagcata ttaatgacca aatgttgctt taatcagtga tgtcgtttct ccacctggat
     9061 ataatacata taataataag taaactgcta cacctgtaat tgcagtacag aaccatataa
     9121 ctgaagcgaa tggcccgaat ttgcggtgta cattatattt atctttaaat gcagtaataa
     9181 tttgaactag gcctagaata ccaccaattg ttgctaaatt aatgtggaaa aataagaaaa
     9241 tcgtataata tttcttaatt gatgctggtc cgccaaatgc tgtattaccg ataaaaatcg
     9301 ttctacttgc atagattaag aaaaaagtta aagcaaaaac agctgcagct aacataacat
     9361 ttttgtgttt atttatttcc cttttccaaa taagtctcca accaatggca attaaaattg
     9421 cactaatgac aatacatgtc gtactaatcg ttggtaaaat tggaacgccc atatttttca
     9481 tcctaactta attaatctag atcaaagtaa gtaatgaaac aatcacagct aacacgaaaa
     9541 agatcactaa ataatttagt gaatatataa acatttgtgt tgcccatttt gtttgatctg
     9601 aatttttctt aaatgttgtt aaacctaatg caatccatcc taaatttaat aatgtagcta
     9661 acactacgaa tacgacacct aaatttatta gtaataaagg tactggcaat aaaataatca
     9721 accagataaa catactgaca cgtgtacgtt taaagccctt aactgatggt aacattggaa
     9781 tatttgcaag tgcatattca tctttacgtt taatagctaa ggcataaaaa tgaattggtt
     9841 gccaacaaaa tacaactaaa aacagcgcaa tcgctgttaa actaatttgt ccttcaattg
     9901 caacccatcc aattagtggt ggtactgctc caggaaaact cccaatcact gtgttccatg
     9961 ttgtatgtct tttagaccat attgagtaat aagacacata acctacaatc cccataagac
    10021 caagtacgcc tgatggtata ttcaataaaa acaaacaaat ttctccaact aacatcatac
    10081 caaaacttaa tagtaataaa ttttgatctg taattctatt atttacagtt ggtctatttt
    10141 gtttactagg cataatacga tcaatatctt ggtcgtaata attatttaac gcacatgcgc
    10201 cacccataat taaagtagat ccaaatagca ttaataaaat ttgaggtatt gatgataaga
    10261 aggaatgatt tgtcattaca actgctagcc atgcgcccgc aaaagccgga attaagttac
    10321 cttgaacaag tcccatttta attatctgtt gcaattcttt gaagttaact ctgctaatat
    10381 tttgtgacaa agtatgctct ttgctcataa tccccctcct taaatttgtt catataagat
    10441 tatgatatct tagattgcat aaaaagacta ggtttaataa aattaaattg tgacaaatta
    10501 acgacaagag aaaatgtcaa ttttgtgaca caaataacat ttaatttatt gctataatgt
    10561 atatgttaga aaattttaat aagtagaatc atgcatctaa aagagattaa tatttaagct
    10621 tcaaatttga gtaaacgtgg attacataat tatcccaata aaaaaatcat tacgattaag
    10681 ttctttttat gtcgtccaca tacaatactt gtaaaattaa atcatatttc ctgcgttgga
    10741 tcccatcttt tcatatccta caatcaggtc tatttatagt atcatctcaa atccggctat
    10801 taattctaat tctcagtgat gcgttttttt atgatggggt gtataaattg tttggcaaaa
    10861 agaatttaaa atggttaggt gtcgtagcaa cgttaatgat gacatttgta caacttggtg
    10921 gagccttagt taccaaaacc ggatcagctg atggttgtgg ttcttcttgg ccactatgtc
    10981 atggtgcgtt gattccagaa ttctttccta ttgatacgat tattgagtta agtcatagag
    11041 ccgtttcagc tttgtcttta ttaatggtct tatggttagt tatcactgca tggaaacata
    11101 taggctatat taaagaaatt aaacctttat caatcattag tgttggattc ttattattgc
    11161 aagcattaat cggagctgct gctgttattt ggcaacaaaa cgattacgtt ttagcattgc
    11221 actttggtat atcattaatc agtttctcat ctgtattttt aataacattg attattttct
    11281 ctatagatca aaaatatgaa gctgacgaat tatatatcaa aaagccatta agacgtttaa
    11341 catggttaat ggcaatcatc atttattgtg gtgtttatac tggtgcacta gtgagacatg
    11401 cggatgcaag tttagcatat ggtggttggc cattgccatt ccacgatctt gtaccacatt
    11461 cagaacaaga ttgggttcaa ctcacgcatc gtatcatggc ttttatagtg tttacgatta
    11521 ttatgattac ttatattcac gctgttaaaa attatccaaa taaccgtact gttcattatg
    11581 gttatacagc tgcatttata cttgttatat tacaagttat cacaggtgca ttatctatta
    11641 tgacaaatgt taacctgata atcgcacttt tccatgcatt atttatcact tatttatttg
    11701 gtatgacaac atactttatc atgctaatgt tacgatcagt aagaagtgac aagcaataac
    11761 aaaaaagcaa accgtaattt taatggcacg cccattaaaa ttacggtttt ttatatcaat
    11821 atttaaaatt aaacctaagc catgtaaaaa cgagattaca cgtcaattgt tgtgtaatct
    11881 cgttttattt taatcatttt agtcagttgc tttttcaatt tcgattaata aatcgcctgt
    11941 cgctattgtg tcaccattat ttacagttac ttgtttaatc acaccgtcaa atggtgcttg
    12001 aattgttgtt tccattttca tagcttcagt aattagcaac ggctgattag ctttcacagt
    12061 ttcacctaca ctaaccttga cttcagttac tgaacctggc atttgagcac cgatatgact
    12121 tggattactc ttatctgctt ttggcttaac gttcgcattt gtatgcacat tttcatcttt
    12181 aatgtaaata cgtctcgctt gaccattcat cgcatagtaa atcgttctat taccattttc
    12241 atctggttca ctaatcgttt ctagtttaat aattaatcgt ttacctttat cgatttcgat
    12301 ttctactgtt tcaccattac gcattccaaa gaagaatgta ggcgtatcaa gtaacgataa
    12361 gtttccgtat tgatttctag tttgaatata ttgttcatat acttttggat atagtacata
    12421 actaataata tcttgctccg taacaggacc ttgttgctct tcttcaagca actcacggac
    12481 tttttcaaaa tcaactggct ctagatattc acctggacga gctgttagtg cttcttggcc
    12541 ttttaaaata accgcttgta aatctttatt aaaaccattt acaggttgtc ctatttcacc
    12601 tttgaagaac gacactactg attctgggaa atctaattta tagccatctg taatcacgga
    12661 ttgttcatca agatcatttt gtaccatata aagtgccata tcaccaacta ctttagacga
    12721 tggtgttact tttacgatat caccaaataa gaaattcact ctgcgataca tatctttgac
    12781 ttcatcaaat ctttcgccta aacctaaact tttagcttgt tgacttaaat tcgaatactg
    12841 tccaccaggc atttcatgtt gataaatttc agtattcggt gatttgatat cactttcaaa
    12901 gtctgaataa taagtacgta cagttgacca ataatgacta agtgactcca taccttcaat
    12961 atcagttcta aggtggcgtg ggaagccatt taatgcataa tataacgaat tggcgcttgg
    13021 ctgacttgtt aaaccactca ttgaagcaac agcagtatca atgatatcga caccagcatc
    13081 tattgcttgt ttgtatgtta ataaaccatt accacttgta tcatgagtgt gaagatgaat
    13141 tggtaaatct acagctgatt ttaactcacc aatcaattcg taagcggctt taggttttaa
    13201 taagcctgcc atatctttaa tcgctaaaat atggaaacct tcacgttcta actctttagc
    13261 tagtttgaca taatactcta aagtataaat gtttgatcgc tcaggattta aaatgtcacc
    13321 tgtataacaa atagtacctt ctgagatttt gcccgcttct tgtactgctt cattggcaac
    13381 tttcatttga tctacccagt ttaatgaatc gaaaattcta aagacatcta tgcctgcttt
    13441 agcactttct tgtacgaatt tatgaataac attatcagga tagtttttat aaccaactgc
    13501 gtttgaagca cgtaacaaca tttggaataa tacatttgga atagctttac gtagacgttc
    13561 aagtcgttcc catgggtttt ccttcaagaa attatatgcc acatcaaatg tagcaccgcc
    13621 ccacatttct agtgagaaac catctttaaa tacgtccgct gttttggatg cgatattaat
    13681 catatcctta gttctaactc gtgtagctaa taatgattgg tgtgcatctc taaaggttgt
    13741 atctgttagt aagacatcat cctgcttttt aacccattca gctacacctt ttggacctac
    13801 ttcatcaagc aattgtttcg taccactaaa tgaagcgatt ttacttgaag atacagttgg
    13861 aattgatgct aattcatagt ctggtttcgg acgtttctca acatttggga aaccattaat
    13921 tgttacatta cctatatatt ctaatgtttt agtacctcta tctagagacg gctgaatgtt
    13981 gaaaagttct ggtgtttctt caataaattt agttgtgtaa tcaccacttg tgaacttttt
    14041 attcttcatt acattaatta agaatggaat attagtttta acaccacgaa tacgcatttc
    14101 tcgtaatgag cgtaccattt tttcttctgc ttgtttaaat gatatcgcgt gtgtagataa
    14161 ttttacgagt aatgaatcat aataaggtga tatctcagca ccttggaaac catctccagc
    14221 atcaagacgt acaccaaagc ccccgcttga acgataagca atgattgttc cagtatccgg
    14281 catgaaatca tttaacggat cttctgttgt aatacgacat tggatggcat agcctaatgt
    14341 tgtaatatct ttttgttgcg gcatattaat ctcttcacca aataaatcgg cacctgctgc
    14401 aactaaaatt tgtgtcttaa caatatcaat tcctgttacc atctctgtaa ttgtatgctc
    14461 tacttgtaca cgagggttaa cttctataaa gaagaattcg tcaccagata ctagaaattc
    14521 aacagtacct gcattgacat atttaatatt ttccatcaat tgaattgcag catcacaaat
    14581 acgttgacgt aatgttgatg ataatccaac tgatggtgca acttctacaa ctttttgatg
    14641 acgacgttgt actgaacaat cacgttcaaa taagtgtacg atatttccat gttcgtcacc
    14701 tatgacttgt acttcaatat gctttggatt atcaatgtat ctttctatgt aaacttcact
    14761 attaccaaat gatttttcag cttctgattt tgctctatgg aaagcatctt ctaattcact
    14821 ttcttcacga acgattctca tacctttacc gccgccacca cttgtggctt taatcattag
    14881 cgggaaacca gcttcttctg caaattcttt tgctaattca tatgatttaa ttggaccgtc
    14941 tgtaccagga ataactggta aatctgcctt gatagccgtt gtacgagctt taactttatc
    15001 tccaaacata tctaaatgtt ctaaatgagg accaataaat ttaattcctt cttctgcaca
    15061 acgacgcgca aattgttcat tttcacttaa aaatccatag ccaggatgaa tcgcatccac
    15121 attcgcttgt tttgctacat caatgatacg ctcaatattt aaataacttt cagcaggacc
    15181 taaatcactt ccaactaaat aggattcatc tgctttatat ctatgtaatg aacttttgtc
    15241 ttcattcgaa taaattgcaa ctgtgctgat gtctaattct gccgccgctc tgaatatacg
    15301 aattgcaatt tctccacggt tagcaacaag taactttttt atttgtttca atagcgatac
    15361 actcctcaaa ctattagaat tttctaacta attagataat aaaattttat cttaaagcgc
    15421 tctgttttgc tatagttatg tttcaaattt tcaaaattta acattcttga gacaattaaa
    15481 acctccgctt cagaaataat aatttcaaaa atgactatgc aacaacaggt agttcacgtt
    15541 tttgttgtga aacattttcg atttctacaa ctctaaaaaa ttaaaaataa aattgcaaaa
    15601 catcaacatt tattatcaat agcgataact ttatcttatc atcatgattc taatttcgcc
    15661 accacattta gtaattttta gtcataaaat ttagttataa ttatacgttg ttttgtttat
    15721 aaaatttgat aatcatgagt aatctcgtaa tatcaaaaca aaaaggaagt taagcgttgt
    15781 ttggttgcct aacttccgtt attgaactca tcagtccaat ttgacaaaga gtatataaat
    15841 agccaatgtt tagcattgct taatgacaga agtcatctcg tactaaatat tggctagtat
    15901 ttttttaatt aaattgtctt cttatatcaa ctttttgttg ttgtttcttt cgttgctggt
    15961 ctactttgat ttgtttacct acaatcagaa gtaaacccat agcaatactt aaactaatca
    16021 ttgatgatcc accaaagctg ataaatggca atggcacacc agttaatgga attgttgccg
    16081 aaataccgcc aatgtttaca aacgtttgac ttccaaagta tgtggcaatc ccaacacaca
    16141 caagtttata aaaatatgat gatgttttat ttgcaaactg gaaggcacga tatacaataa
    16201 agaactctaa agtaataact agcaatcctc cgattaaacc taattcttcg caaataattg
    16261 caaaaataaa atctgtatgt ggttctggta aatagcccaa tttcattgca ctatttccta
    16321 atccttttcc aaatacgcca ccgttaccta tcgcaagcaa tgaattggaa atatggtatc
    16381 cagttcctga ttcgaattgg aatggatctg ttagcgtact aaatctggca gttaaataac
    16441 ttggtaacca accagccatt aatgcaatga caaatactac taagaatcct agcactgctg
    16501 gtataccaaa tcttaggact ttgtttaccc caattcctga ataaaaaatg atcgcaacta
    16561 aaataattaa tattagtaat gtttgcccta cgtctttttg taggaaaact aaaaacgtac
    16621 aacctaatgc taatacaata ggacttaaaa ttaattttgg tttacttaat actctaggca
    16681 tttttttact gatcataaat gggatatata atatgattgc aatttttaat aactcagatg
    16741 cctgtaagtt cataaatcct aagtttatcc aacttttaga accattaata tctttaccta
    16801 ttactaacgt cagtaataat agtgagacga tagttataat catacctttt tgcactttaa
    16861 tattactcag taatttaaca ttcattaaaa atgcaataaa aaatacaatt ataaaactca
    16921 ttatgacata tgctaattgt cggttgtaaa aatacgttcc tggaacatcg ataccaccag
    16981 tcaatgtgcc tttagttgct ggaaccatac ttgcactata taccatcact aatccaatta
    17041 aactcaatac aatatatgta actaataacg gataatcaat aaactttgag gttttaccaa
    17101 tataccgtaa aatacttcta aaattcttca tatattcagt catccaattc tacaatttat
    17161 acttcaaatg atttcgtttt atactttaat tttaaaaatc catgttactt attatataca
    17221 tgacacagtc ataatcacaa atattaacag gtctcttgag gtaatttata gaatatttgt
    17281 ttacttaata tcgtgaattg ctaaaaccca ataaaagtga ctgacatgtc atcaagaaga
    17341 caacataaca gccactttaa tcagtaatta aactataaat ataaaatcgg cgcagctcaa
    17401 ttgtcgatat ctcacgccat tttaatatgc ttatatatta aaccttaaac aagtgtaaaa
    17461 gcttcatgta attttgaaag ttctttctca agacgtaaca taatttgttt gccatcttcg
    17521 cggtcaacta aacctaattt aacagcaaaa tcaacttctt tttgaagtcc aaacatttgt
    17581 gtatctagta cttcttcata taatgggcat gaaggtaatg ttaaattatc tagttgaact
    17641 ttaatcagat gcaagatttc atcagcatct ttagtcaatt gtttcaaagc tgcatttttc
    17701 attgttgctt gtttcgccat gacacgctcc ccctctatat aaattctcaa gtcttactaa
    17761 aagtttatct tactaaatat taaaaagcaa gtagatttaa aatgtgtcgt tagcaatttg
    17821 tgttaaaata atgctatata cattgatagg gagtgggaaa atgatacaaa ttaaaggagc
    17881 cgtaaagttt cctataacat tagatagtac gacttggatt ttcgatgata gaaaagttag
    17941 catcgaagat ttagaaaaag gtgtatttga tggttcaaaa ccaatcgact ttgaagataa
    18001 tcatgaatgg aatcgtgcta tcctagaagg acaaactaat ccgcctactt taaattcaga
    18061 aataaaatat aaaaaacgtg ccatgttaga aggttcattt gtcattaata tgacaccatt
    18121 tttcaaacat tccgaaccac atgactctgc aactactatt cgattatcaa atgagcatga
    18181 tcatttagat ataccgatag atctattacc ctatttattt ttccaatttg caaaagacgg
    18241 taaacgactt tacagcgata atggtgtaga tagttttatt tacaatcaag aagatggtta
    18301 ttcatatcga atcaaccatg taactcatat tgaggtgatt taatttgaga caagtgcaat
    18361 gcattatctg tgatgcaaaa gtatttatag atgagcgcac aactgagtct aaacgactta
    18421 aaaacaatcc tattcgaaca tttatgtgtg atgattgtaa aagtcgatta gacacaccta
    18481 aacaacgtgc gcaacactat ccgctcgatt aatatctgaa taaacaagtt agaaatttga
    18541 aaatgtgaaa gtaacaggaa ataatagcat ttaaacactt tttcaaatat tatatgccaa
    18601 aagagaagct aagcaacttt catcgctcag cttctctttt attatgttaa attattcgtt
    18661 ttcttgcatc atttgtttga tacgctttgc ttctttttca cgaacatttt tgtttaaaat
    18721 tttctttctt aaacgtatac tttctggtgt aacctcaaca agttcatcat cattaatgaa
    18781 ttgtaacgct tcttccaatg ttagaatacg cggtctattc attgtttgtg tttggtcttt
    18841 cgttgcagaa cgtacgttag tttgatgttt tgttttagtg atgttaacag ttaaatcatt
    18901 ttcacgatta tgttcaccaa caatcatacc ttcataaact tcagtaccag gttccatgaa
    18961 gtttacacct ctatcttcaa gtcccaaaat ggcataagta cttgcagaac cttgatccat
    19021 tgaaattaat gcaccattac gacgaccgcc aatttgtgct ttaatacgtg gtctaaattc
    19081 ttcaaatgta tggttaataa taccgtaacc tcttgtcatt gacataaatt cagtcgtata
    19141 accaatcata ccacgagccg gtacattaaa gattaaacgt gtaagtccat tatcagttgt
    19201 agtcatatca accatttcac ctttacgtgc acctaatgat tcaataacag cacctgcatt
    19261 ttcttgtggc acttcacatt gcacacgttc aaatggttca cacattacac catctatttc
    19321 tttaataatt acttgtggtt ttgaaacttg taattcataa ccttcacgac gcatattttc
    19381 aataaggatt gataaatgca attcaccgcg accagcaact acccatgtat ctggagaatc
    19441 tgtgttagaa actttcaaag atacatctgt ttctaattgt tgatttaaac gttcttgaat
    19501 ttgacgtgct gttacaaagt caccttcacg gccagcaaat ggagaattgt taactttaaa
    19561 tgtcatttca agagtaggct catcaatacg tagaactggc aatgcttctt gatggtcatg
    19621 tggtgttaca gtttcaccaa cattaatgtc ttccatacct gaaacagcaa ttaaatctcc
    19681 agcttgtgct tcttcaattt ctaaacgttt taatccaaag taaccaaaga ttttagttac
    19741 acggaagttt ttcactgtac cgtctaattt aattagtgat acattatctc cgacacgcat
    19801 tttacctctg aatacacgac caataccaat acgtccaaca taatcattgt agtccaacaa
    19861 tgctacttgg aattgtaatg gctcatcact gttatcaatt ggagctggta cataatcaat
    19921 aattgtttca tataatgatt gtaaattatc atcttgcttt tcaggatcta agctagctgt
    19981 accatttact gctgaagcat aaacaacagg gaattctaat tgttcatcgt ttgcttctaa
    20041 ttcaataaat aaatctaaaa cttcatctac aacaccctct ggacgtgctg atggtttatc
    20101 aattttatta acaacaacaa caggtttcag gttttgttct agcgcttttt taagtacaaa
    20161 acgtgtttga ggcattgtac cttcatacgc atctactact aagacaaccc catcaaccat
    20221 tttcataata cgttctactt ctccaccaaa gtctgcatgt cctggtgtat ccaaaatatt
    20281 aatacgtgta cctttataat caacagccgt atttttggct agaatcgtaa ttccacgctc
    20341 tctttcgata tcgttagagt ccattgcacg ttcatcgaca tgttcatttt ctctgaatat
    20401 accagattgt tttaacaact catctactaa agttgtttta ccatggtcaa cgtgagcaat
    20461 aattgctata ttgcggacat cttctctttt attagtcata taaatagttt ccttctttct
    20521 taagtaaaat cccactttta attacaactt ttttattata tcatataatt gagaaaagat
    20581 aagcaaaagg tggggatagg atgaaacaaa aaaaatctaa aaatatcttt tgggtatttt
    20641 ctatattagc tgttgttttc ttagtattat ttagttttgc tgttggtgca tcaaatgtac
    20701 caatgatgat tttaacattt atattactcg ttgcaacctt tggaattgga tttactacaa
    20761 agaaaaaata tcgagaaaac gattggctat aagttatatc gacaataaca cgcgttgatt
    20821 taagaccaca catgttcatg atgaacattt aactggtttt atattggcgc gtgtttttat
    20881 tatttaaggt ataaaagcgt aatatgttac ctaattgtta ggactacata ttacgctttt
    20941 ggtaattatt ttgattttct tttaaatcgt tgttcatgta attgtattaa cgcatcatgg
    21001 tggggctcta aataatcatt gctaatttct tgatggagac cacgatttcc aactaagatt
    21061 gaatttggac cactaattgt taatggttct cctagtaaat tggaagcttg tccatttact
    21121 tcatataaaa taaccaatcc gccagcaaaa tcccatggtt gaagtcttgg cgtcatatat
    21181 gcttctaaat tacctgtagc aactgaaacg atttcaagcg ctgcactacc atatgccctt
    21241 gcacttctag aatcattaac aatttcttta aagatttctc ctaaaattgg tttagttaac
    21301 cagttcggat tgatcccaat aatgctttgt cttagattag aatcattcaa tggtttcaag
    21361 ggttggctac cacgatatgc accttcccct actttagcat gatataagac atcagccata
    21421 acatcatata caaaacctgc ataaggttta ccatcgatat aaataccaat tgaaattgcg
    21481 aaattttctt gttgatgaac aaaattcaat gtaccgtcta ttgggtcaac aatccatacc
    21541 gtacctttgg aagtatcgat gtcatgacca tgcccttctt cacctaatac ttgatgattg
    21601 ggatatgttt ctaaaattgt atcaaaaatg aaatcttctg ttgctttatc tacatttgta
    21661 acaaggtcat tcggatttga ctttgtttca attgttaaat tttgctccat caattgttta
    21721 attctaattc ctgcttcttg aataagtcct tgggcaaatc cataaagtgc cataacatca
    21781 tctcctaaca tctttattat acatcaacat tttataaaaa taacttctta tgataaaaat
    21841 gaaaatacta attttaatga gagcgtctta agccgcaatt gataaaacat atgctacaat
    21901 atttttaaac actatacagg aggtactcta tgacaaaata tacatttaaa cctaaagatt
    21961 tcaaagcgtt caacgtagaa ggcttagacg cacgaatgga agctttaaac gaatacatac
    22021 gaccacaact ccgtgaatta ggagaatatt ttagtgactt ctttacaagt caaactggtg
    22081 aaacatttta tcctcacgta gcaaagcatg ctagaagaag tgtgaatcct cctaaagata
    22141 catgggttgc ttttgcaaca aacaaaagag gctataaaat gttacctcat ttccaaattg
    22201 gtatgtttga agatcaactg tttgttatgt ttggaatcat gcatgaagca aaagataaag
    22261 caacacgtgc aaaagttttt gaaagaaaat ttaaagctat tcaacaatta cctgatgatt
    22321 atcgtgtttg cttagatcat atgaaacctg ataaaccatt tattaaagat ttaacggatg
    22381 atgatttaat agaagcgata caaagagcca tcaatgtgaa aaaaggtgaa ttctttatag
    22441 cgcgtgcaat cacaccacaa gataaaagat taaaaagtga caaagcattt attgcatttt
    22501 tagaagaaac cttcgatcag ttcttaccat tttattctgc ataaataact ttgtttaaat
    22561 aatagagcac ttaatcacat ccatgatttc gtgctctttt ttcttaatat taaatcgaac
    22621 gttcaacata ataattcata cttttaaaaa aattaaaata aatttaggtt gacctaaaca
    22681 ttttattagg ttattatatt gtccataaga agtagaggtg agtcaaaatg aataataaac
    22741 gacattcaac aaatgaacaa ttaagtttag acgaaataaa caatacaatt aaattcgatc
    22801 atcgcagttc aaataaacag aaatttttat catttcttgg acctgggtta ttagtcgctg
    22861 ttggttacat ggatcccgga aactggataa catcaatgca aggtggcgcc caatatggct
    22921 atactttgct attcgtaatt cttatttcaa gtttatcagc aatgttactt caaagcatga
    22981 cagtgagatt aggaatagca acaggtatgg acttagctca aatgacacga cattatttat
    23041 caagacctat tgctataatc ttttggatca ttgcagaact agcaattatc gctacagata
    23101 ttgctgaggt tattggtagt gctattgctc ttaatctcct atttaacata cctttaatcg
    23161 tcggtgcact aataactgta cttgatgtat ttttactact ttttataatg aaatatggtt
    23221 ttagaaaaat tgaagctatt gttggtacat taattttcac agtgttattc atctttatat
    23281 ttgaagtcta tatttcatca ccacagttga atgctgtgtt aaatggattt ataccacata
    23341 gtgaaatcat tacaaataac ggcattctct atattgcatt aggtattatt ggcgctacaa
    23401 ttatgcctca taatttgtac ttacattcat caattgtaca atctagaaca tactcaagac
    23461 ataacaatga agaaaaagcg caagcgatta aatttgctac gatagattcg aacattcagt
    23521 tatcaatcgc atttgtagtc aattgcttat tattagtgtt aggagcatca ctatttttca
    23581 actcaaatgc tgacgattta ggtggtttct atgatttata tcacgcctta aaaactgaac
    23641 ctgtactagg tgcaacaatg ggtgcaatca tgagtacatt atttgcagtt gcattattag
    23701 cgtcaggtca aaattcaacg attactggta ctttagcagg acaaattgta atggaaggat
    23761 ttttaagatt acacatacca aattggttaa gacgtttaat tacacgttct cttgctgtca
    23821 ttcctgttat cgtatgctta atcattttta aaggtaatgc agctaaaatt gagcaactac
    23881 ttgtattctc tcaagtattt ttaagtattg ctctgccatt ctgtttaata ccattacaat
    23941 tggctactag taataaagac ttgatgggac ctttctataa taaaacatgg gttaatatca
    24001 tttcatggac acttattatt attctaagta ttttaaatgt ctatttaatc gttcaaacat
    24061 tccaagaact acaaagttaa aaataaaacc attaaaatgt tggttgtatc taataattaa
    24121 caacttaaca ttttaatggt ttttcttttg ctatgaacat attaatgata aatatactca
    24181 tcgtccttat gtgttgtttc ttttgattct tttttagaaa tcaacataat catcacgata
    24241 acccataaga caattgcaat taaattaaga gccatcaaac ttacaattat cgcgataaca
    24301 aaaagtataa tcgataattt tctacgtcct ttaatacaaa ttgtcgcaaa tataccataa
    24361 agtgttgaaa ttaataaaat aattgctaat gcacccattg ttgtctgaag catatctaaa
    24421 gaaaccttgc caccgttctg agataattgc ttcgctaatt cttcacgttg tgatgcatta
    24481 ccacttttta aagaaaaata ggatagcccc ataactaata aataaataat actaatacta
    24541 tttgcaatcc aagccatgat taattcaggt tttctattca tcttaaaatc ctttctttgt
    24601 ttaatatttt aataagtata ctacgagcct atagatatac gcacattaaa ttacttgtat
    24661 attaacatat ttttcatcta atttttataa taaacatctg aattcagatt gatgataatt
    24721 taggtgtata gtaatgagaa atcgtcattt aaaattatga cacctaaaca ctttaattaa
    24781 acgttatcga taattaacct catataaaaa cccagattaa taagcaaaag acctctgctt
    24841 actaatctgg gtatcatcaa tatttattct tgctcttttt tgtccttaac ttcacttgaa
    24901 ttgcgttctt cagaaatatc attgttgtaa atttgacgtc gctcttcaaa attcattcga
    24961 cgttgactaa ctgcgctagg ttgatttttc ctacgttctt tcattcgttg gttataagct
    25021 ttacgttttt cttttttctt agctttaatt tcagcttttt ctcgcttttg ttgtgctttc
    25081 aattcttttg gatcaacttc aggttctgca taataaccat catcttcaat acctagttct
    25141 tcagtacttt tttgtttgta tttagcctgt ctggataata ctgatggttc ttcctcagtg
    25201 cgctcatcat gattatattc actttcaact gcacgtttag gaaattgatc atatttatct
    25261 ctttcctgtt cttgatcata tacagtattt gatttatcca attctttttc atatttaggc
    25321 acatcataat aatcatcttt cggttggggg cgctcataca caggttcacg ataatcataa
    25381 atcggtccgt tatactcttc ataatattgt ggcgcaacca tttcaacttt atcttttctt
    25441 ataaatagca tcatcgcaat aataaagaat aatatcggaa tgattaaagt aacgataagt
    25501 aaaactaaag gaatcgttac aattgctgat attaagaata agaaacctga caagattctt
    25561 atattcatag atatgagagc taaaaatgaa atcaacaaac aaacaataaa gtaaacgata
    25621 atagcccata taccattttg taaccatatt acaaattgtg ttgtattata gccatttcca
    25681 gctaataatt gctggataaa tgcattattg tttaatgtat tttcaagatt agcaatcgat
    25741 gtgttattac tgaatgaaac aagtgctata aacatcgtaa tgacagtaag cactaataac
    25801 atcacccaac ataaccaacc tagaactttt tcagttaatc tacttactgg acgtttaatt
    25861 tgagtaaatt gttctccagt cattcgttac aactccttat agtacttatc ccgttattat
    25921 aactaaatat acagtaaata actactattt atgattttat tttaatgaca ttttgaaatt
    25981 caaaaagttt tcattgtatt cacttaaaac ttcaggtcct aaatctttat aaacttcaag
    26041 gcgttcttgc tctttcttag tcggataaaa acgatggtcg tctttaatct ctttaggcaa
    26101 caattgtcga gcagccttgt ttggcgttgc atagcctacg aattctgtat tttgcttgtt
    26161 atttttagca tctaataaaa aattcataaa tttatatgca ccctctttat tttgtgccgt
    26221 ttttggaatt accatattgt cgaaccataa attcgatcct tctttaggaa taacataatt
    26281 atatttatcc ccttcttgca ctagaggtgc tgcaacacca ctccaaacaa ccgctatgtt
    26341 accttcattt tgttgaagca tcatggtaat ttcatcacct acgacacctc ttacttgtgg
    26401 tgctagtttg gttaaatctc gctctgcttc ttttaaatgg tgcgaattac ggtcattaag
    26461 attataccca agtttattca aactcatgcc tataatctct ctagcaccgt caactagtaa
    26521 aatttggttt ttaaatttag gattatacaa tgacttccaa ctatcaaatg att
//
LOCUS       DAMQYT010000015        25769 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMQYT010000015 DAMQYT010000000
VERSION     DAMQYT010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25769
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.015"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            140..2245
                     /gene="clpL"
                     /locus_tag="PGA33_000543"
     CDS             140..2245
                     /gene="clpL"
                     /locus_tag="PGA33_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HDG6472382.1"
                     /translation="MNNGFFNSDFDSIFRRMMKDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGGNHSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(2314..2484)
                     /locus_tag="PGA33_000544"
     CDS             complement(2314..2484)
                     /locus_tag="PGA33_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDG6472383.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     TEQHTANHFPGK"
     gene            complement(2497..4491)
                     /gene="feoB"
                     /locus_tag="PGA33_000545"
     CDS             complement(2497..4491)
                     /gene="feoB"
                     /locus_tag="PGA33_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDG6472384.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKYLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVGAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(4503..4730)
                     /locus_tag="PGA33_000546"
     CDS             complement(4503..4730)
                     /locus_tag="PGA33_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDG6472385.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(4946..7435)
                     /gene="farE"
                     /locus_tag="PGA33_000547"
     CDS             complement(4946..7435)
                     /gene="farE"
                     /locus_tag="PGA33_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HDG6472386.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVVFAVLSALTLLPALISIFHKRI
                     KVNKLQSKFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFASVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDAHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQL
                     NSKQQATNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            7580..8128
                     /gene="farR"
                     /locus_tag="PGA33_000548"
     CDS             7580..8128
                     /gene="farR"
                     /locus_tag="PGA33_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HDG6472387.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEDLREIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKDE
                     KIDWPGEDIDNIFHRLINIKIK"
     gene            complement(8205..8372)
                     /locus_tag="PGA33_000549"
     CDS             complement(8205..8372)
                     /locus_tag="PGA33_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472388.1"
                     /translation="MRYVISIIMGIVLAIWSFKQLSQSHLDSGFIFFFIVYVLCISCF
                     NSDKHDKNKKR"
     gene            complement(8484..10028)
                     /gene="pruA"
                     /locus_tag="PGA33_000550"
     CDS             complement(8484..10028)
                     /gene="pruA"
                     /locus_tag="PGA33_000550"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HDG6472389.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(10218..10817)
                     /locus_tag="PGA33_000551"
     CDS             complement(10218..10817)
                     /locus_tag="PGA33_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDG6472390.1"
                     /translation="MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            11056..11247
                     /gene="cwrA"
                     /locus_tag="PGA33_000552"
     CDS             11056..11247
                     /gene="cwrA"
                     /locus_tag="PGA33_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HDG6472391.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            11487..13895
                     /locus_tag="PGA33_000553"
     CDS             11487..13895
                     /locus_tag="PGA33_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDG6472392.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLILTETTTFKAVPGHGIEATIDH
                     HYILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
     gene            14186..14392
                     /gene="copZ"
                     /locus_tag="PGA33_000554"
     CDS             14186..14392
                     /gene="copZ"
                     /locus_tag="PGA33_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDG6472393.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
     gene            complement(14482..15480)
                     /locus_tag="PGA33_000555"
     CDS             complement(14482..15480)
                     /locus_tag="PGA33_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDG6472394.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNFIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(15503..16657)
                     /locus_tag="PGA33_000556"
     CDS             complement(15503..16657)
                     /locus_tag="PGA33_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDG6472395.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            complement(17084..18592)
                     /locus_tag="PGA33_000557"
     CDS             complement(17084..18592)
                     /locus_tag="PGA33_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDG6472396.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIERGV"
     gene            complement(18604..19467)
                     /locus_tag="PGA33_000558"
     CDS             complement(18604..19467)
                     /locus_tag="PGA33_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HDG6472397.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            complement(19504..20631)
                     /gene="crtQ"
                     /locus_tag="PGA33_000559"
     CDS             complement(19504..20631)
                     /gene="crtQ"
                     /locus_tag="PGA33_000559"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HDG6472398.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            complement(20637..22130)
                     /locus_tag="PGA33_000560"
     CDS             complement(20637..22130)
                     /locus_tag="PGA33_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDG6472399.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            complement(22123..22611)
                     /gene="crtO"
                     /locus_tag="PGA33_000561"
     CDS             complement(22123..22611)
                     /gene="crtO"
                     /locus_tag="PGA33_000561"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HDG6472400.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            complement(22786..23553)
                     /locus_tag="PGA33_000562"
     CDS             complement(22786..23553)
                     /locus_tag="PGA33_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDG6472401.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            complement(23914..25725)
                     /locus_tag="PGA33_000563"
     CDS             complement(23914..25725)
                     /locus_tag="PGA33_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDG6472402.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
BASE COUNT         8301 a         4885 c         3980 g         8603 t
ORIGIN      
        1 ttcataaagt tttaaaaata ttctgtttta tcacctacta ttagtggaaa agtacaattg
       61 caattgtata tagtttgcat aacgcttcaa aagtaatttc ttttttgttt agttcaaaaa
      121 aatttagagg tgatgttata tgaataacgg ttttttcaat agcgactttg attcaatttt
      181 tcgaagaatg atgaaagata tgcaaggttc aaatcaagtc ggaaacaaaa agtactatat
      241 taatggtaaa gaagtttcac ctgaagaact agcgcaactc acacaacaag gtggcaatca
      301 ctctgctgaa caaagtgcgc aagcttttca acaagcagca caaagacaac aagggcaaca
      361 aggtggcaac ggcaattatt tagaacaaat tggtcgtaac cttacgcaag aagcacgtga
      421 cggtttatta gatccagtca ttggtcgtga taaagaaatt caagaaactg ctgaagtttt
      481 aagtagacga actaaaaaca atcctatatt agttggagaa gctggtgttg gtaaaactgc
      541 gattgttgaa ggtttagcac aggcaatcgt tgaaggaaat gtaccagcag caatcaaaga
      601 caaagaaatt atttctgtag acatttcatc attagaagct ggaacgcaat atcgtggtgc
      661 ttttgaagaa aatattcaaa aattaatcga aggtgttaaa tcttcacaaa atgccgtact
      721 attctttgat gaaatccatc aaattatcgg ttcaggtgcc acaggaagtg attcaggtag
      781 caaagggtta tctgatattt tgaaacctgc attaagtcgt ggtgagattt ctattattgg
      841 tgcaacaaca caagatgaat atcgaaacaa tattcttaaa gatgctgcat taacgcgcag
      901 atttaatgaa gtgcttgtta atgaaccaag cgctaaagat actgttgaaa ttttaaaagg
      961 tattcgcgaa aaattcgaag aacaccatca agtaaaatta ccagatgacg tattaaaagc
     1021 atgtgttgac ttatcaattc aatatattcc acaacgatta ttaccagata aagcaatcga
     1081 tgtgttagat attacagcag cacatttatc tgcgcaaagt ccagctgtcg ataaagttga
     1141 aactgaaaaa cgaatttctg aattagaaaa tgataaacgt aaagcagtaa gtgctgaaga
     1201 atataaaaaa gctgacgaca ttcaaaatga aatcaaatca ttacaagata aattagaaaa
     1261 tagtaatggt gaacatactg ctgttgctac agttcatgat atttcagata ctattcaacg
     1321 attaactggt attccagttt ctcaaatgga tgataacgat attgaacgtt taaaaaatat
     1381 ttctaatcgt ttaagaagta aaatcatagg tcaagatcaa gctgtagaaa tggtttcacg
     1441 tgcaattcgc cgtaatcgtg ctgggtttga tgacggcaac cgtccaattg gcagtttcct
     1501 atttgttggc cctactggtg ttggtaaaac agagcttgct aaacaattag caattgattt
     1561 atttggtaat aaagatgcac tgattcgact tgatatgagt gaatatagtg acacaacagc
     1621 tgtttcaaaa atgattggta caactgctgg ttatgttggt tatgatgaca attcaaatac
     1681 gttaactgaa aaagtacgcc gtaatccata ctcagtcatt ctatttgatg aaatcgaaaa
     1741 agcaaatcca caaattttaa cattgttatt acaagtaatg gatgatggta atttgactga
     1801 tggtcaaggt aatgtcatca actttaaaaa tacaattatt atttgtacat caaatgctgg
     1861 ctttggcaat ggcaatgacg ctgaagaaaa agatattatg cacgaaatga aaaaattctt
     1921 ccgccctgaa ttccttaacc gcttcaacgg catcgttgaa ttcttacatt tagataaaga
     1981 tgcattgcaa gatatcgtca acttattatt agacgatgta caagttacat tagacaaaaa
     2041 aggtattacg atggacgttt ctcaagatgc gaaagattgg ttaattgaag aaggctatga
     2101 tgaagaatta ggtgcacgtc cattaagacg tattgttgaa cagcaagtac gtgacaaaat
     2161 tacagattac tatttagatc atacagacgt taaacatgtg gatatagatg ttgaggataa
     2221 cgaattagtc gtaaaaggta aataacgaca ctttaacata tcgcgcatca aaaatgagca
     2281 tcaggtcgcc cttgcctgtg ctcatttttt taattatttc cctggaaaat gattcgctgt
     2341 gtgctgttct gttccacaac aatcacgatt aatgtcacat gtaccacatt ttccttgttt
     2401 tgaacgcttg aaaaatttta ctagtgtata taaggcatat ccgaaaattg ctaaaaaaat
     2461 taaaatgtta ataatgactg acactttaac cactccttaa acaaataaat gtccgacttg
     2521 ataaaaaatg aatgttaaga catatgcagt gactagagga taggcaactg caagtgccgt
     2581 ccatttccat gaataagtct ctttacggat tgctgctact gtagaaacac aaggaatata
     2641 caatagtata aatatcataa atgcatacgc agatagcggt gtgaattgat tttgaatcac
     2701 attaacaagg cctgcatcac ctgatgaata gataatcgcc atcgaactta cgataacttc
     2761 ttttgctaaa aatcctggca ctaacgtagc acctgcttgc catgttccaa atccgagcgg
     2821 ttgcactaac ataccaaaga aactaccaac catatgtaaa aaactttgat tgatattcac
     2881 attgatacca tgtggtccta cataacttaa tagccaaatg actactgagc cgccaaaaat
     2941 aaatgtacct gctttacgaa caaagccctt agccttttcc caagtactac gccacaacgt
     3001 tttaatggaa ggcacacggt atgttggcaa ttccacaata aagattgcat tatcattttt
     3061 taaaatcgtc ttagtaagta ctgtactgac taaaaatgcc ataataatac ctaaaacata
     3121 caggcttaat actactaaag attgattctc tttgaaaaag atacctacga acaacgcata
     3181 cactggcagt ctagcagagc atgacatgaa tggtgcaatt aatatcgttg ttaaacgctc
     3241 tttttcattt tcaatactgc gcgcagccat aatactcggt acattacaac caaatccgat
     3301 aatcattggt ataaaagact tcccgcttaa accgaacgat tccataatac gatccattaa
     3361 aaccgcaata cgtgccatat aacccgaatc ttctaataaa gatataaaga aaaagagcac
     3421 aacaatttgt ggtacaaaga ctaatactga tcctacacca gcaataatgc catctgtaat
     3481 taaatcttgt aaaaatggta taacaccaag ataattcata atcgtcttca cactatctgt
     3541 aaatgtacca cctataaatg catcgagttg atccgacaaa ggtgtgccaa tccatgtaaa
     3601 tgtagtttga aagatcaacc acataattgc tagaaagata ggcatcccta tatatttatg
     3661 tgttaatatc ttgtctattc tagagctgaa atattgctta tcttcatctg gatacgttac
     3721 cacgtcttgc aataacgtct caatataatg attgcgtata cgctccatct ctcgacgaac
     3781 agatacagcc cctacttgtt cagcaacttg atcacgtaaa ctcgacaatt tatttacaac
     3841 ctctgaatta agttcgtttg caatttgcat gttatttaat aagaattgaa tcgcaataaa
     3901 cctagcttga tacttatcat gagatgtctc tgtcattatt atttgacaca tatttttaat
     3961 tgtctcttca atcttctcac cataattgat tttaaaatgc ggttgatacc cttcccctag
     4021 atgctttatt tcgccaagta aatattttgt tcctttgcct gtacgtgcca caactggaaa
     4081 aataggtgtt tttaactttt tcatcaattt atgataatcg atttttatcc cgcgctttgt
     4141 agctacatca atcatattta atccgatgta tattggttga ttaagttcta acaattgtac
     4201 tgttaattgc atatttcttt ttagttgact cgcatcaaca atgttaatga ttcctgaaaa
     4261 tgaatcgttt aataaatagt ctgtcactac agtttcatct ttagaaatcg gcgataaatc
     4321 atatgtacct ggtaaatcaa ttaattgtcc tacattttct ttaagtttcc ctactttttt
     4381 ctctaccgtt acgccactcc agttgcctat atattcatac gaaccagtta aagcgttaaa
     4441 caaagatgtt ttaccaacat taggatttcc taaaatacaa taattttcca ttcgtccggc
     4501 tcctattctt ctaatgcaat agaacaagca tcgcaatgtc taatacttaa ctgttgtccg
     4561 tttacttcaa taatacatgg ccctttaaat aaacattttt gtttaatcgt tatgatagcg
     4621 tcatctgtta acccaaaggc acttagacga tacaacatat tctcattagc aatatccatt
     4681 cgctttattt tataagcctt attcatttca ccatttttaa tgtttaacat actatttcgc
     4741 tctcctatta gaaataataa tcattatcac ttaaaaatca taacccttaa aattgtagct
     4801 cgcaatactt tatttaaata attttcattt ttcatgtaaa atttgtgaca ttgcaaaaat
     4861 gttttgtata atcacttcca tgcaaaaaac ctccgcttca aatgtcattg atagacattt
     4921 aaagcggagg catgtacatg ttattttatg atttatttcg tagatttttt tctaacaatt
     4981 gattgagctt ttcaatattt ttattcaact gttctatatt cgttgcttgt tgttttgaat
     5041 ttagttgatg cttgttttga taatctatta aagcatcata taagaacaat gaacttgctg
     5101 attgctgttt cacttcttgg tacaatgcca tcgttttatc atctagtact attgaatgtg
     5161 catcatcttg cccatgtgca tcttgatgtt tcgatacatt tgttgcacct tgtggatata
     5221 ctttaaaggt tgtgtcgtat gtttccttac tatctttagc ttcagattcc tgtgattcaa
     5281 ccgttttata tttttcaagt gcatgtcctt caatatcaac tcgtggaata atgcgattca
     5341 accatgctgg taaataccac gaaccttttc caaacaattt cgttaatgca ggaattaaca
     5401 tcattctgac tacgaaggca tcaaagagta caccaaacgc taatgccata cccattgatt
     5461 taatcatgac atcttcttgg aatacaaacg caaagaagac actaaacata attaatgcag
     5521 ctgctacaat aacaggaccg ctttctttca atcctacttt gatagaataa tcattatccc
     5581 ctgttttact atattcttca tgaattcgcg acataaggaa gacttcataa tccatcgcta
     5641 atccaaataa gatacctata gtaataaccg gtaaaaatgc tagcattggt cctgtcgttt
     5701 caataccaaa cagacctttc ataaaaccat cttgcattac taatgttgta aatcctaatg
     5761 ttgccattaa tgacaagacg aatcctaaaa ctgcttttaa tggtattaga attgaacgga
     5821 agacaatcat taataagaaa aatgctaata caacaatgac tgaggcaaat aaaggtatcg
     5881 cctcatttaa ctttttagac atatcaatat taatgacact ttgtcccgaa atctccgttt
     5941 tgaacccata tttatcttgt gcatctttat gataatctcg taaatcatgc actaaatcat
     6001 ttgtactctc tgcattaggc ccttgcttag gtatcacgac catcaaagcg taatcattat
     6061 ctttactcat ttgtggtggc gtaacgatat ctacattttt cttatcttta atatctttat
     6121 atacagactg taaatcttgt tgtaatcctt gtggatcatc ctttttatct ttcacattta
     6181 tcaacatcgg tatttggcca ttaaatcctt caccaaattt atccgagata atatcgtaag
     6241 cttttttctg tgtagaatct gctggtttaa caccgtcatc tggaatacca agtcgcatat
     6301 gactaactgg tattgcagct gctactaata tgattaaacc tagtaatact gccgcaagtg
     6361 catttcctgt aataaattta gaccatggcg tatcaatatc ttttttgaat ttagactgta
     6421 atttattcac tttaatgcgt ttatggaaaa tgcttattaa tgcaggtaat aaagttaaag
     6481 cgctaagtac tgcaaaaaca acactaattg ccgaagcaaa tcccattacc gctaagaagt
     6541 caatgcctac taatgataaa ccacatactg caattacaac tgttacacca gcaaaaacaa
     6601 ctgcactacc tgctgttcct attgcaagac caatgccttt aatgtaatct gtttcagttt
     6661 tcataacttg tcgatatctg aataaaataa ataatgcata atcgatacca actgctagtc
     6721 caatcattac ggctaatgtc agtgtgacat ttggtatatc gaatgcataa gttaacaaac
     6781 tgataatacc tacaccagag gctagaccaa tcaatgcact tataattggt aatcctgcag
     6841 caatgactga accgaatgtg attaacagta caacaaatgc aacaataata ccaactagtt
     6901 cagaattacc gcctacttct gtagatgtca ttcctgtacc agttaactct gtttgtacat
     6961 tatgattgtc acgtaagtct ttcaaatgac ttttaacatt atctctagag ccatctttta
     7021 aagatgtttg actaacgtca tatgtgatat ctgcaaatgc agttgtttta tctttactaa
     7081 tttgcttatt ttcataagga tctgatattt tatcaatgtg cttgtcatct tttttaatat
     7141 catctaacgt tttcttaata tctttagtaa tgttcggttg cacaatacca tcatctttag
     7201 tcgtcttaaa gacaacacgt atttgtgcct tttcactatc ttgattaaaa tgtttttcaa
     7261 tctttttatt cgtatctaac gactctaatc ctgtcatttt aatatcattg tcaaatttcg
     7321 gtgcatttgt agcaagtggt atcaatattg cagctacaat cactatccat gcaatgaccg
     7381 cggaccattt atgttttgcg atgaatgtcc ccatcttata taaaaatttt gccaaagtat
     7441 attgcctcct tttaaaatca acgttatagt ttaaatatac agtgtagatt attgttcgat
     7501 tatagtatct atccccgacc tcttaaagaa tcaattggaa aattttgtat attaaactac
     7561 acacaaagga gaaatgtaga tgaaagagac tgatttacga gttataaaga caaaaaaagc
     7621 attgtcgagt agcttgctac aattgttaga acagcaatta ttccaaacga ttactgtcaa
     7681 tcaaatttgc gacaacgcac tcgtacaccg tacaacattt tataaacatt tttatgataa
     7741 atatgatctt ctagagtact tgttcaatca attgactaaa gactactttg ctagagatat
     7801 cagtgaccgt cttaatcatc cattccaaac gatgagtgat acgattaata ataaagagga
     7861 tttgagagaa atcgcagaat tccaagaaga agacgctgaa tttaataaag tattaaaaaa
     7921 tgtctgcatt aaaattatgc ataacgatat caaaaataat agagaccgta tcgatattga
     7981 cagcgacatc ccagataatc tcatatttta tatttatgac tcgttgattg aaggttttat
     8041 acattggata aaagatgaaa aaattgattg gcctggcgaa gatattgata acattttcca
     8101 tagattaatc aatattaaga ttaaatagta gatgagaaac tcatgagcgt taccaacatt
     8161 cataataaaa acgatagtgt acacgttaat gaattcgtgt actactatcg ttttttattt
     8221 ttatcgtgct tatcgctatt aaaacaactg atacacaaca cataaactat gaagaaaaaa
     8281 ataaatccgc tatctaaatg actttgactc agttgtttaa atgaccaaat tgctaataca
     8341 attcccatta ttattgaaat aacgtatctc acattcttat acctataatc cttttctaaa
     8401 aatatggttg ctattactta atttttaaag ttataaataa aaagagccaa ccgcaatgga
     8461 tggcccttgt tcattatgaa gcattagaac atttctgaaa caaccttttg ttctaagaag
     8521 tgtaataagt agtctggact acctgtttta gcgtccgtac ctgacatttt gaaaccacca
     8581 aatggatggt atccaacaac tgctgaagta cagcctctgt taaggtataa attgcctaca
     8641 tcaaattcgt ttaccgcttt aatccaatgc tcgcgattat ttgtaatcac tgcaccagtt
     8701 aaaccgtaat ctgtatcatt tgcaacctca attgcttcat caaaatcgtt aactttcaca
     8761 aagccaacaa ctggaccaaa aatttcttct tgcatgattc tatctttaga tttaagtcct
     8821 gaaatgattg ttggttctac aaagtaacct tttgaatcat cagtgccgcc accttgttct
     8881 aatttacctt cttctttacc aatctcaata taatttttaa tcttatcaaa ttgtttttta
     8941 ttaataactg ggcccatata cgtattgtct acagtattgc ccaacgttaa ttcttttgtt
     9001 aatttgattg atttctctaa tacttcgtca taaacgtctt tatgcacaat tgcacgtgaa
     9061 catgctgaac atttttgacc agaaaaacca aatgctgacg ttacaatagc ttctgctgcc
     9121 atatctgtat caatattttc atcaactaca atggcatctt taccacccat ttcagcgata
     9181 acacgtttca agaagttttg accttcttga acaacggcac tacgttcata aattctagta
     9241 cctgtcgcac gtgatcctgt aaatgtaacg aaatgcgtat ctttatgatc aactaagtaa
     9301 tcaccaattt ctttcggatc accaggaaca aagttaacta cgccttttgg taatcctgct
     9361 tcttctaaaa tttccattaa tttataagcg atataaggtg tatcctcagc aggtttcaat
     9421 aacactgtat tacctgccac aactggtgct aaagttgtac cagccataat cgcaaacggg
     9481 aagttccacg gcggaattgt aacacctgta ccaattgatt tatagaaata tttattgtgt
     9541 tcaccttcac gatcaagtac tggcttacct tgagccaagt ccatcattga acgtgcatag
     9601 tattcaataa aatcaatacc ttcagctgca tcaccaactg cttcatccca tggcttacct
     9661 gcttcataaa ccataattgc tgcaatttcc gcttttcgac gacgaataat tgccgaaaca
     9721 cgtaacataa gctctgcacg atcatttgct gaccatgttt tccaagattt ataagcttcg
     9781 tttgctgctt taaacgcatc ttcaacatct tgttttgttg cctttgatgc atttgcaatc
     9841 acttgtgatg tgtctgcagg attgattgat ttaattttgt catctttgaa aatcttctct
     9901 ccattaatca ctaatggtat gtcttgacct aattcttttt ccacgtcttt caatgctttc
     9961 ttaaacatat ccacattttc ttggactgaa aaatcgtaac caggttcatt tttaaattct
    10021 actaccatgt acacttaccc cctataaatt ttgaaagtgg tttaaccctt tgatttaatg
    10081 atataacatc atttaaactc attttactat gattaaggtt agttttgcaa tcgctttcat
    10141 ttttatgttt tatcacttat tctcaagtat tttgaaattg attggttact ttttaaaatt
    10201 tatatgggtc gcaactgcta ctttatcgtt tcgtcattta atgtttcgga tggtaggtca
    10261 ttatcaattt tacgaacgac tttacaaggg tttccaaccg ctaagctgtg tggcggaata
    10321 tctttagtga caacactacc agcaccaatc acactgcctt ctccaatcgt cacccctggt
    10381 aacacggcta catgaccgcc aaaccaagta ttactgccaa tatgaatggg tccggctttt
    10441 tcaaaacctt catttctatg atggaaatta agtggatgtg tcgctgtgta gaatccacaa
    10501 ttaggtccta taaaaacatt atcgccaatt gtaatctgtc caccatccat aaagtaacaa
    10561 ttggtgttta catagacatt ttttcctagt ttaacgttcc aaccataatc tgtatcaaaa
    10621 ggaatcgaaa tacttacatt gtctgttgtt gtttgaaata attgatcaat taattccttt
    10681 cttttatttg tagcactcgg tcttgtatga tttaattcaa agcaaatatc tttcgctcgt
    10741 gcacgttcat tgattaagta ttgatcaaag tttgcatcgt accatttttc tgctaacatt
    10801 ttttcttttt cagtcattac acctttcaac tcctaataac ttatttactt gtttaaaagt
    10861 taatcaaata aaccttcgcc tatgcaacta atacgctata acattatgaa atcatgacct
    10921 tatcaccctt atctatacaa ttctcgcatc aaatactgct aaagtagtag ataaattcaa
    10981 tactacagac gcattcattt tttaatctat taacgtacaa tgtgagtaag agaaatataa
    11041 aggagtatga tagcgatgag aatattaatt acaggcacag ttgctatctt aatcattcta
    11101 ggtttggtca aaacgataca agattacgaa atgacaaacg acacgagtcg tcagttgtca
    11161 gacaacaaag atgatgataa agtcatccat cttaataatt ttaaaaattt acatgcgaaa
    11221 gaatttaacc catctgattt cttttaagtc acctaagaat tgcaaatcca gaagtcattt
    11281 aagttttacc tttcattcat acatccttta atattaatta cgacttcttt tatatagatg
    11341 ctaagtagag agattgttgt gcaatgtttg cacggcaatc tctctttttc tttttaaaat
    11401 tggtaaaagt aaaacgcaac gattgactta tatacctata gggggtacat tagacgtgta
    11461 acaatgaatc acagggaggc aataatgtgg ctaatacgaa aaaaacaaca ttagatatca
    11521 ctggtatgac ttgtgccgca tgttcaaatc gtatcgaaaa gaaactgaat aaacttgatg
    11581 acgttaatgc ccaagtgaat ttaactacag agaaagcaac tgttgagtat aaccctgatc
    11641 aacatgatgt ccaagaattt attaatacga ttcaacattt aggttacggt gtcgctgtag
    11701 aaactgtcga attagacatt acaggtatga cttgtgctgc atgctcaagc cgtattgaaa
    11761 aagtgttaaa taaaatggac ggcgttcaaa atgcaacggt caatttaaca acagagcaag
    11821 ctaaagttga ctattatcct gaagaaacag atgctgataa acttgtcact cgcattcaaa
    11881 aattaggtta tgacgcgtct attaaagata acaataaaga tcaaacgtca cgcaaagctg
    11941 aagcgctaca acataaattg attaagctta tcatatcagc agtattatct ttaccactat
    12001 taatgttaat gtttgtacat cttttcaata tgcatatacc agcactattt acgaatccat
    12061 ggttccaatt tattttagct acacctgtac aatttattat tggatggcaa ttttatgtag
    12121 gtgcttataa aaacttaaga aatggtggcg ccaatatgga tgtacttgtt gctgttggta
    12181 caagtgcagc atatttttac agtatttatg aaatggttcg ttggctaaat ggctcaacaa
    12241 cgcaaccgca tttatacttt gaaacaagcg ccgtactaat taccttaatc ttattcggta
    12301 agtatttaga agctagagcg aagtctcaaa caaccaatgc gcttggcgaa ttattaagtt
    12361 tacaagctaa agaagcacgc attttaaaag atggtaatga agtgatgatt cctctaaatg
    12421 aagtacatgt tggagataca cttatcgtta aaccaggtga aaagatacct gttgatggca
    12481 aaattattaa aggtatgact gccatcgacg aatctatgtt aacaggtgaa tctatccctg
    12541 ttgagaagaa tgttgatgat actgtaattg gttcaacgat gaacaaaaac ggtactatta
    12601 ctatgacagc aacaaaagtt ggcggggaca ctgcgttggc aaatattatt aaagttgtcg
    12661 aagaagctca aagttctaaa gcgccgattc aacgattggc agatattatt tctggttatt
    12721 tcgttcctat cgttgttggt atcgcactat taacatttat cgtgtggatt actttagtta
    12781 caccaggtac atttgaacct gcacttgttg cgagtatttc cgttctcgtc attgcttgtc
    12841 catgcgcatt gggacttgct acaccaactt ctattatggt aggtactggt cgcgctgctg
    12901 aaaatggtat tttatttaaa ggtggcgagt ttgttgaacg cacacatcaa attgatacca
    12961 tcgttttaga taagacgggt accattacaa atggtcgtcc agtcgtgaca gattatcatg
    13021 gtgacaatca aacgctacaa ctacttgcta ctgctgaaaa agattctgaa cacccattgg
    13081 cagaagccat tgtcaattat gcaaaagaaa agcaattaat attaactgag acaacaacat
    13141 ttaaagcagt acctggccat ggtattgaag caacgattga tcatcactat atattggttg
    13201 gtaaccgtaa attaatggct gacaatgata ttagcttgcc taagcatatt tctgatgatt
    13261 taacacatta tgaacgagat ggtaaaactg ctatgctcat tgctgttaat tattcattaa
    13321 ctggtatcat cgcagtggca gatactgtca aagatcatgc caaagatgct ataaaacaat
    13381 tgcatgatat gggcattgaa gttgccatgt taactggcga taataaaaac actgctcaag
    13441 ccattgcaaa acaagtaggc atagatactg ttattgcaga tattttacca gaagaaaaag
    13501 ctgcacaaat tgcgaaacta cagcaacaag gtaagaaggt tgcgatggtt ggtgacggtg
    13561 taaatgatgc acctgcatta gttaaagctg atatcggtat cgccattggt acaggtacag
    13621 aagttgccat tgaagcagct gatattacta ttcttggtgg cgacttgatg cttattccta
    13681 aagccattta tgcaagtaaa gcaaccattc gtaatattcg tcaaaatcta ttttgggcat
    13741 tcggctataa tattgccggt atccctatag ctgcattggg cttacttgcg ccatgggttg
    13801 ctggtgctgc aatggcacta agttcagtaa gtgttgtcac aaacgcactt agattgaaaa
    13861 agatgcgatt agaaccacgc cgtaaagatg cctagattcc ttaataatga aggattcgtt
    13921 ggtgattctg agataggcta gtgattggct ctataatgtc gcggtttaca gttggatctt
    13981 cgctccaact gcataagagc cactaattat tataaataat aagtacacta cggtttacag
    14041 ttggatcttc gctccaactg cataagagcc actaattatt ataaataata agtacactac
    14101 ggtttacagt tggatcttcg ctccaactgc ataagagcca ctaattatta taaataataa
    14161 gtggctctaa aaggaggtga atgctatgtc acaagaaatt ttaaatgttg aaggtatgag
    14221 ctgtggtcac tgcaaaagtg ctgttgaatc tgcattaaat aatattgacg gtgtcacttc
    14281 agctgacgtt aaccttgaaa atggtcaagt aagtgttcaa tatgatgaca gtaaagttgc
    14341 tgtatctcaa atgaaagacg caattgaaga tcaaggttac gatgtcgttt aattaggcaa
    14401 tattcaacgt catcaacacc aaattaaaaa atcgaactga tgagaatccc aacaatccaa
    14461 attatctcat cagttcgatt tttaatttac tcgtaaccta gtatctccag tctgcaatac
    14521 atctaatgtt gcatctaatg catcgacaat tagattttta actgcagctt cagtataaaa
    14581 cgcaatatgt ggtgttaata tgacatcttc cctgtcaatc aacgattcta acaatggatc
    14641 gttcagtgtt ttgccccttt gatcacttgg gaaaagtttg cgttcaaatt catacgtatc
    14701 aagtgctgca cctttaatca caccattgtc taatgcgtct aataacgcct tagtatctac
    14761 taaagaacct ctcgcacaat tgacaaatac tgcgcccttt ttaaaatgtt taaataattc
    14821 agcattaaat agataatgat tatatttcgt tgcaggtaca tgtaatgtca cgatatcagc
    14881 accttcaacc gcttcctcaa tcgtatcttt gtaatcgaca tacgttgcaa ttttagcatt
    14941 aggaaacggg tcgtatgcga ccacatcact ttgataacca ttggcaaata tatcggctac
    15001 tacacggcca attcgacctg taccaataac agctactttt aaatctttaa tggatttcga
    15061 taaaatagta ggttcccatc taaaatcatg ctcccgcact ttcgtttgaa tttgattaaa
    15121 atgacgaacc acattaatag cctggttcac agcaaactcc gcaattgaat tcggagagta
    15181 tgacggcaca tttgacacaa taaagttata cttgtttgct aactccaaat catatgtatc
    15241 aaatccagca ctacgttgtg cgatttgttt aatacctagt tcatttaatc gtttataaac
    15301 atgctctgat aatggtattt gttgtgatag cgataagcca tcataaccag cgacaccttc
    15361 aacattgtca tcagttaatg cttctttagt aatatctacc tcaacatgat gtttctctgc
    15421 ccacgccttg atataaggca tatcttcatc acgtacactc atgattttaa tttttgtcat
    15481 tttaacatca cccttaactt tattattcat ataaatatgc tagttctgtt aatcttattg
    15541 cagcttcgtc taatttctgg tcatctaacg ccaatgaaat tctcacataa cgattaccat
    15601 tctctccaaa tggtttccct ggagcaacaa gtattgactt ctcttgcact aaaaattgct
    15661 caaattgctc gctgtcataa ccaggcggtg tttccaacca tacatatatg ccacctttag
    15721 catgaacaaa tggcaaatca gcttttgcaa gcatggcttc gaatcggtca cgacgtgttt
    15781 taaatacatt gctttgttct tctaaaaaat catcataatg attcaaagca tatattgcgg
    15841 catcttgtaa tgcaccaaac atcccagcat ttgtgtgcgt ttggtacttt ttcaaagctt
    15901 gaatcatatc tttattacca actgcaaaac cgactctaaa acctgacatg ttataacctt
    15961 tagacaagga ataaatttca atcgcaacat ctttcccatt ttccgaagca agtatactag
    16021 gatttttagc gtcgaaaccg aaagcaccat aagcaaaatc atgcacgatt ttagtgtctg
    16081 tacctttaaa tttagctatc gcttcatcaa aaacttcttt cgtagctgtc gatccagttg
    16141 gattatttgg atacgttaaa taaatgagtt ttgttttatc tattatttgt gaatcaactt
    16201 tggaccaatc tggcaaataa tgtggcggtt ctaaattaag cgggactggc ttgccatcag
    16261 ctaaaagtac acctgctaaa taatccgtgt agcctggatc aggtagtaat acatagtctc
    16321 ctggattgat aacacatgtt ggtactgcca ctaatccatt ttttgtacca tataaaatgc
    16381 atacttcatc ttctttatct aacgtcacat tatattgtct ttgataaaaa tctacaatag
    16441 cttgcttgaa cgcttcttta ccatgaaaag caccatattt ttgattttca ggaatagtta
    16501 gtgctttttg aaaatgatca ataatacctt gtggcgtggg cccatcaggg attccaactg
    16561 ccatattaat taatggcaat ggtccatgtt cgattttacg tcccatcgtt ttcccgaaat
    16621 aactatcagg gatatttgct aatttgttag agatcatcaa attcctcctc tatcattaaa
    16681 catagcctgg gcgactatca taatcctaac aacttgtatc actctcattt agatggttac
    16741 aatgacatcg ccattcaccg ttatgttcaa cagaacttat gacacacgtt gtattgaatg
    16801 aatttatttt cattttaggt aggtataata ttattgtcaa tattaggaat tttcagatta
    16861 atatgcactc aatcgttatg atttaactgt catgcatatc cgcatgcgca accagttaga
    16921 tatgcttata taaagtataa cgcccatcaa ggtacgtatt caaacgtgaa ccttaacagg
    16981 cgtcattcat tgttaaataa aacttcttaa gcacatactt atttcactat gccttttacg
    17041 ttccccttat acttttctca catctttctc ttagactact cccttatacg ccccgctcaa
    17101 tatctttaat catttcatct acagttattt tcgcactcgt taagacaata ggaacgcctg
    17161 cacctggatg cgtacttgca cctgcaaaat ataaatcttt ataatctcgc gatacatttt
    17221 gtggacgata ataattactt tgcgctaaag ttggcattaa accgaatgcc gaaccaaatt
    17281 tcgcatgata cgtttgctca aaatcatttg gcgtaaagat tgtttctgaa acaatatgcg
    17341 attttatatc ttcaaatact tcaatcgttg ctaatttacg ataaataatt tcctttattt
    17401 gttgcgtcaa agcttcatct gaccaatcga ttccgctacc tgttttaagt tccggcgtcg
    17461 gcattagcac ataaatacca gttttgcctt ctggcgcaag tgatttatca gcgaccgctg
    17521 gtacatacac ataaatagaa ggatcatatg ataaacgtcc ctcaaatatt tcttcaatat
    17581 tgcctctaaa gtcatctgaa aaaataacat tatgaagtct cacttgatct gtcacatcaa
    17641 tatctatacc gatatacatt aaaaatgctg aacaagagta atctaagtct gcaattttat
    17701 gtggtggata ctttttaata ggtgcaaaat ctggcattaa tgattccgca acactaggga
    17761 aatcagccgt acataaaatt ttatcaaatt ttcttatgtc accattcact tttatcgcat
    17821 cggcccgttt gaatttagga tcaataataa tttgctcaat ttcagcattt agttcaatat
    17881 taacgcctaa gtctttattt aattgcgcta gcccttgagc catgccatac ataccgcctt
    17941 taataaaatg cacaccaaac atcatttcaa tcataggaat aattgaatat agtgacgggc
    18001 ctcgttttgg atcaattcct atgtataacg tttgaaacgc taaaagcttt tgtatctttt
    18061 cgttatcaat ataatgttca attagctgat ctgcatgatt taacgttttt aacttagcac
    18121 cttgcacaag tgacgtcata ttataaaagt cactcggttt gcgatacgtt ctttctaaga
    18181 aatagcgacg tgcaatttca tattttttat aaacatccgt taaaaaggac ataaaaccat
    18241 gcgttgaacc aggttctata ctttctagca tttgctgtaa ttcagctaaa tctgtaggca
    18301 ccgttatacg atcatcgtgg tcaaaataca catcgtaaat ataacgtaat tgtctcaatt
    18361 caatataatc ttcataattt ttaccacacg ctgtaaaaac atctttataa acatctggca
    18421 tcatgacaat tgtgggaccc atatcaaatg taaagccgtc tttctttaat tgattcatac
    18481 gcccgcctac attattattt ttttcaaata tcgtcacttc atgaccttga gaagcaatac
    18541 gggctgccgc tgctaatcct gtgacacctg caccaattac tgcaatcttc attattcaac
    18601 cacctatatt ctatgatatt tactatttat ttcatgaaac aactttgcct ttttcctctt
    18661 atccacaaaa acacgttcat gtaatgtata gttagcctgt ctcacttcgt ccagtatttc
    18721 aatatatata cgtgctgcta attctatgat tggttgtgct tcaatactaa atactttgat
    18781 ttgatccata acatcttgaa aatctttttc tgcgatagct gcataatatt cccataagtc
    18841 aatataatga ttattaacac cattttggta cacttcagca atatcaactt catattgctt
    18901 taatcgttgc ttactaaaat atatccgttc attgtcaaaa tcttcaccga catctcttaa
    18961 tatattaatc aattgcaacg attcaccaag tcttcttgcg acatcgtatg tctgatgtgt
    19021 ttcatgatca cttaaaatcg gcgtcaatac ttcacctact gtaccagcaa caccataaca
    19081 atatccgaat aattcagcgt ccgtttcaaa cattgtaaaa tgttgatctt tatatacagt
    19141 atcaatgaga ttataaaaag attgaaaggc gatattttta tgttgtgcaa catgctgaag
    19201 cgccatcatg attctacgat cactttgaaa gtgatgatgt tcatatgggt atttttcaat
    19261 agattgtata tcttctttta tttgatttaa aaattgaata tcgccataaa catctatact
    19321 gtcatcaatt ttacgacaca cagcataaat tgcccaaacc gcttttcttt gatcttctgg
    19381 taacaagtca aaagcgtaag aaaagctttt tgaatgtttc ttcatgattt tatgacaata
    19441 tttaaaattc atatccatca ttgtcatact agtcctccta tattgaaatg ccattacctt
    19501 gatttattgt tctttagata tagaatattg acgacctttc cattcaacta caccataacg
    19561 atgcgtttgt ttccaagatt ggatgaaaat tttagtaaaa aacataaata acaatggatg
    19621 acataccatt aataaattag aaaattggcc gacccttcga tgcagataaa taaattgtgt
    19681 agtatagctc aagtaaagtg ctaccatttt tcttacagac atttggcgat attttaaact
    19741 aagacatagc cctaaaatac tcgctataga accaaacaac cacaacacaa ttgctgtcat
    19801 gatcttaggc tttgtgccac ctgccccagt tgacaaatgc tttgtccatc cttcttgtaa
    19861 tgactgaaat ccttcttgat acatgcgaaa tgcaacaaat ggaaaccctt cataaactgt
    19921 tacgggcaat gattgtgaag tatatgcact tcctaaagca aatccttcaa taatatgacg
    19981 gtttgcactt ttatgacctc cagttgcata ataatcttct ttatttgtta atgtcaccgg
    20041 tccaaatgca tgctggttag tccgaccgtc gtctaaggta gaaaatacat tcataccaac
    20101 gactgtcatt aaattaaata tcgctgaaaa cccttcgtag aaacgctttg ttatatgata
    20161 aggctgtacg cttaacaatc ctttttcacc ttgtaattga tactgattaa tcaacgtttc
    20221 aacagcatct ttccttaaga aagttacatc agcatctaca aaggcaatgc gattcgtaca
    20281 tgcatgcgtc acaccttgat aacaagcatg tgatttccca taccatttgc cgtcagtatc
    20341 atcaacaaca tctactacag tcgcaccata tgaacgtgct acacgtgctg tttcatctgt
    20401 cgatccgtca ttcataacaa tgacatctac tggaacttgc tgttgtatta tcgaatgtag
    20461 taaatgacct attctttttt cttcgtttct agccggaata ataattgtta atgctttatg
    20521 attgaagttc agcgttttcg cctttagctg atgtctacga ttaaatatca atgcaccaca
    20581 cgccatagac atggtcacta ttactgttaa tattcgtgat aaccatttca tagatatcac
    20641 ttcctattct tcgcttctcg cgcgtttatt ttgtctgcga cttgttgccc acttaatgta
    20701 accattggca tgccaccacc aggatttact gatccaccta caaagtacaa gttttcaaaa
    20761 tactgacttt ctttaggaaa tttaaatcct ttgtttttct ttttatctgc tacaacacca
    20821 tatattgcac cacgattaga acgataattt ttttcaatat cctccggtgt ccaaacatct
    20881 tcataaataa tgtgttttct taaatcagta agtcccattt tttctaattt atccaaaatt
    20941 ttatccctaa acttcgcata atcttcagtg gtaaaaggct gatcttgaat atatggaata
    21001 tgtggtaaga ctttgatatt ttcataacct actggcgctt gtgtgtgatc agttttattc
    21061 gtatttacta gataaatggt cggatcatct ggcaatacct tttcatgaaa aacttgttga
    21121 taattgagat aagcattttc cgtaaaaaag aaattatgat gtgctaattg cgggtattgg
    21181 caagcaacac ctaaatgcat cacatatcct gagcttgccg gctcaaattc cctctctaat
    21241 ttgtttaatc gttgagtatc aaggtgaatt aaatatttat aagtaggtat gacttccata
    21301 tttgaaataa tataatctgc ctttacaaac tcacctgtat ctaatctgac acccgtcaca
    21361 cgtctttgat atgttttaat attgtccaca cgtgcacctg tatgaattgt gacaccttct
    21421 tcacgcgcta gcttttccaa ggcattggct aaatgatgga ttccaccttc tacataccaa
    21481 aggccttgct cttgttgcat atggaataac atagataata cagctggcgc atcgtatgat
    21541 gaagaaccta catatttgat aaaatagcct aacatttgtc gcaagtatgg attcgagata
    21601 cgcttgtcta tggcctgttg catagtatgt acataatcat aattaataag agcatttaat
    21661 ggcccatgaa atttaataat ttgagatagt gtatctaaac catagttgaa ataccctttt
    21721 tcagtaatac gatcgattcg tcttgtataa ttcaaataat tttgcagttc ctctatatcc
    21781 tgtttcgaca atatcgcatt atgctgacct gtttctttaa taccttcata caaatcgata
    21841 gtcgttccat ctggaaaaaa gctacgccat tgatgtggca atcgcttgat tgtaacgtag
    21901 tctgacattt gcttcttgct atattcgaat aatttttcaa aaatataagg catcgttaaa
    21961 atagatggac ctaaatcaaa gccaaagcca tctgattcat gacgattcac tttgcctcct
    22021 atatgattat tttgttcata taatgagacc gaatagccac tttgtgccat tcgaattgct
    22081 gcagaaatcc cacctaagcc accaccaata acgatgatat gtttagtcat gacgttcacc
    22141 tcttcgtttt aatatgcgta gtaactgcgt taatctcggt cgattatagc gttgcacaat
    22201 aatgattgga acattagcta tcattgcata gaaaatattt atatacttta ctaaacgagg
    22261 gcctttattg aatatgatga ctggaagtat cgatatccaa tgaatcaact ctgccctctt
    22321 tgtctctata atcatttttt caatttcatc agtattgatt gtcattaaat gacgctgatc
    22381 atatatattt tgattaagct gatgaccatc taaaatctta tgtttccatt ttcttacgta
    22441 aaaccatttg ttccaaaatt ttccatgctt ctcaaagtta aatgatttaa atattatgta
    22501 cttctgacga aaatacttat caggaattct tgtacctaaa tttgctatat ttagttgaac
    22561 aattaaccaa tacatactac aaaaaaatgc tgttttaata tattttttca tggttttcat
    22621 ctaaattgaa tcactctcaa tcatactgac atagtataaa ttatcacttt attacttagt
    22681 tccctaaaga tcagtgaata aaccgctctc atttatattt atataaaaca aaaacgccaa
    22741 tactctcatg ttagagaata ttgacgtcat ttgatttaca tttgattaat gaatgaaatt
    22801 atatgaacct gcttggtttg ctgaaattgt acgagacgta accacaccag caccatgtcc
    22861 atagttcatt tctgaaacac gaacagaacc gttgctgtta acgccttcaa cgtaagcaac
    22921 atgaccgtaa tagccttgtg ttgtttgcat gatagcacca acttttggtg tattgttcac
    22981 tgtatagcca gatgatgcag ctgcgttagc ccaattactt gcgttacccc atgttgaacc
    23041 aattttccca ccaacacgat caaatacata ataagtacat tgtcctgaag tatataagtt
    23101 acttcctgat gcataaccat tagaaattga acgaccattt gaagatggcg ctgcagttgt
    23161 agtcacatga acattattac ttgttgtgct ataacttgca cctgaaccac cagtataata
    23221 gttatttgtt gctgtttgac tattgtttgt atagttattg tattgatatg aatgattata
    23281 gtagttattg taagatgcat tgttataact ataagtattg tagttgttgt agtagtatgt
    23341 gttgttttgc gttaattgac ttggattcca atttcctgtc caagtgtaat gataattacc
    23401 ttgtgcatca attgtatacg tatagctgta tgattgagcg tcattagaat tatatccatt
    23461 gttattttgt tctgcggctt gtgcatcatg tcctgcaaat gcgatagtgg caagtcctgc
    23521 tgtagcgatt gtagctgtaa cgattttttt cataattaaa tatcctccca ataatcaaaa
    23581 cactctatac atatagtcat tagcgactca attattttaa attttatatt ttttattttt
    23641 ttagtatgca aacaattata acttttatag ttttgcgaca aaacaaacta taacagagat
    23701 taggtgtctt gcgggctttg ttatcataat gtaatctgtt cctaataaag cttcattgaa
    23761 tgtaatatac caattagaat gtttacaaca ctttttctgc accgttacta tgggatttga
    23821 aaataaaaaa gcactaaact tttaatgaaa tacatcattt ttgataataa atatcagaag
    23881 taatgtaata tttcaaaagt ttagtgcatc aaattatttc ttatttgtag catgtgtttc
    23941 catcgttttt acaatcaaat cagtcagcgc tttactacct gcatattcta agtgaatacc
    24001 gtcatatgca aagtattccg gatgacctgc agaagcttta taccaatcga ctagatgtac
    24061 attagagcgc ttttcagctg cctcataaat taatttattt attctacctt cataatctct
    24121 aggtactcta atagaaacta aatatatgtc tgcttttcca aaactatcca atagttcatt
    24181 taattgatct ttcgtaaatg ccccatttgt accaagctct actacaactt tttgaccttt
    24241 tttagcatag tctttatatt gcgatttcac aattggtgta gcatcaacga gttgccgtcc
    24301 aactttacca tcaatttgtg catttggtat tttcttagta aagacattac caatatccac
    24361 catgaccgag tcaccaatta atagtggtga tgacttttta atatcctctt tgctattcgc
    24421 tgtctgctta tcctcttgct tatctttttt aacgactttt ttcttcgttg ttttaaattc
    24481 cgtcttcttc tctttcttct cggcttcatg ttgtttgcca agtgcatcaa actgtccact
    24541 gagcacaacg atagacggaa ccaataatag gataactaac actgttctag caaattgccc
    24601 cttcttttta ggtaaaaatg caaaagcttt aaatcctttt ttacgtatag gtgtttcaat
    24661 aaagcgatac gaaatttcag ccattaacgc tgttaacaaa atttctataa tataaacgta
    24721 taccggtatt tgtccttgta cgtaataact gttcacaaaa acaatgatag gataatgcca
    24781 taagtataag ctatatgatc gtttacctat aattagtaaa ggtttcatac ttaaaaattt
    24841 agcaaataaa ctagaaggat gtaccgcaat tgcaataatg aataaagttg caaatgatat
    24901 aatgtaaaat cctccattat agatccattg atcttggtct ccaactataa agaacaaagt
    24961 cattagaacc gcaaaaccag atatccctat aatatctaat gatacgacaa tctttttaga
    25021 aatatctttt ttcaaagcaa acggaggcca aataaatgct aatatacaac caagcaataa
    25081 agtttgcagt cgtgtatctg tcccaaaata cacacgtgaa ttatctccag tgatgaaatg
    25141 aatcactatc ataagtccta aagaaatcaa cgatacaata aatagcgttt gaataatatt
    25201 tctcggttta aatctatgta ataagaacgt gataaccaat gggaaaagca agtaaaattg
    25261 ttcttcaatg gctaaagacc ataaatgttt gagtggttca atagcaaatt ggttaaaata
    25321 atctacattc tgtgaaatgt accaccagtt tgaaacatag aatatagctg caatagcatc
    25381 tcgtttcatt tgtataatca attccggttt aaatatcagt gtgaacgtaa gcacgacaca
    25441 gattaaaaat aacactgccg gaatgagtcg tttcaatcgt cgcttccaaa actctagcaa
    25501 atcgattttt tgcgtccgat aatactcact tatcaacaaa cttgttatta aataacctga
    25561 aataacgaag aatgtatcta ctcctaaaaa gcccccactt aaccattgtg cattcaagtg
    25621 ataaataatg attcctataa ctgcgaatgc cctcaatcca tctaatccag gtaagtatcg
    25681 cggggaatac attttttcta aacgtttaaa gtcttttgta tccatgttaa taaacgcccc
    25741 atttattttt ctctattttg tagcatatc
//
LOCUS       DAMQYT010000016        24833 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMQYT010000016 DAMQYT010000000
VERSION     DAMQYT010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24833
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.016"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..1072)
                     /locus_tag="PGA33_000564"
     CDS             complement(<1..1072)
                     /locus_tag="PGA33_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HDG6472403.1"
                     /translation="MIDNFMQVLKLIKEKRTNNVVKKSDWDKGDLYKTLVHDKLPKQL
                     KVHIKEDKYSVVGKVATGNYSKVPWISIYDENITKETKDGYYLVYLFHPEGEGIYLSL
                     NQGWSKISDMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKND
                     YPSGYSHGSIRFKYYDLNEGFTEEDMLEDLKKFLELFNELASKVTKTSYDSLVNSIDE
                     IQEDSEIEEIRTAQKDKTLKEVEAPKGIIPKYKKGVSKTTKNDSEIEKSNKENKLTGK
                     VGEKLALNYFNELIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDKAVEK
                     FIEIKTSTSSSIEEPFFMSLNEM"
     gene            complement(1394..1873)
                     /gene="rlmH"
                     /locus_tag="PGA33_000565"
     CDS             complement(1394..1873)
                     /gene="rlmH"
                     /locus_tag="PGA33_000565"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDG6472404.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(2241..4559)
                     /gene="adsA"
                     /locus_tag="PGA33_000566"
     CDS             complement(2241..4559)
                     /gene="adsA"
                     /locus_tag="PGA33_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HDG6472405.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWQVSNAAEQHTPMKAHAVTTIDK
                     ATTDKQQVPPTKEAAHHSGKEAATNVSASAQGTADDTNSKVTSNAPSNKPSTVVSTKV
                     NETRDVDTQQASTQKPTHTATFKLSNAKTASLSPRMFAANAPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDINKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSKSGKDTQPIGDDKVMDPA
                     KKPAPGKVVLLLAHRGTVSSGTEGSGRTIEGATVSSKSGKQLARMSVPKGSAHEKQLP
                     KTGTNQSSSPEAMFVLLAGIGLIATVRRRKAS"
     gene            complement(4787..5587)
                     /locus_tag="PGA33_000567"
     CDS             complement(4787..5587)
                     /locus_tag="PGA33_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDG6472406.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAAHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(5977..6765)
                     /locus_tag="PGA33_000568"
     CDS             complement(5977..6765)
                     /locus_tag="PGA33_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDG6472407.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPTSILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGAEYQLSEISSDSVKYEQTYDDFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(6766..8100)
                     /locus_tag="PGA33_000569"
     CDS             complement(6766..8100)
                     /locus_tag="PGA33_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472408.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLNSDFILFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(8093..9919)
                     /gene="walK"
                     /locus_tag="PGA33_000570"
     CDS             complement(8093..9919)
                     /gene="walK"
                     /locus_tag="PGA33_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDG6472409.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(9932..10633)
                     /locus_tag="PGA33_000571"
     CDS             complement(9932..10633)
                     /locus_tag="PGA33_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDG6472410.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
     gene            complement(11253..11325)
                     /locus_tag="PGA33_000572"
     tRNA            complement(11253..11325)
                     /locus_tag="PGA33_000572"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11290..11292),aa:Asp,seq:gtc)
     gene            complement(11333..11407)
                     /locus_tag="PGA33_000573"
     tRNA            complement(11333..11407)
                     /locus_tag="PGA33_000573"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11372..11374),aa:Glu,seq:ttc)
     gene            complement(11837..13120)
                     /locus_tag="PGA33_000574"
     CDS             complement(11837..13120)
                     /locus_tag="PGA33_000574"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDG6472411.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            complement(13398..14798)
                     /gene="dnaB"
                     /locus_tag="PGA33_000575"
     CDS             complement(13398..14798)
                     /gene="dnaB"
                     /locus_tag="PGA33_000575"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDG6472412.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            complement(14830..15276)
                     /gene="rplI"
                     /locus_tag="PGA33_000576"
     CDS             complement(14830..15276)
                     /gene="rplI"
                     /locus_tag="PGA33_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDG6472413.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            complement(15273..17240)
                     /gene="gdpP"
                     /locus_tag="PGA33_000577"
     CDS             complement(15273..17240)
                     /gene="gdpP"
                     /locus_tag="PGA33_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HDG6472414.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIILFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            complement(17255..18193)
                     /locus_tag="PGA33_000578"
     CDS             complement(17255..18193)
                     /locus_tag="PGA33_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDG6472415.1"
                     /translation="MGVAYVFSKIQPKATIIATIALVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LVIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            complement(18484..19452)
                     /locus_tag="PGA33_000579"
     CDS             complement(18484..19452)
                     /locus_tag="PGA33_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDG6472416.1"
                     /translation="MTNYTVDTLNLGEFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     regulatory      complement(19512..19610)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(19815..20144)
                     /locus_tag="PGA33_000580"
     CDS             complement(19815..20144)
                     /locus_tag="PGA33_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDG6472417.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     gene            complement(20141..20836)
                     /locus_tag="PGA33_000581"
     CDS             complement(20141..20836)
                     /locus_tag="PGA33_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDG6472418.1"
                     /translation="MTTHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIVL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMEQ"
     gene            complement(21486..22772)
                     /gene="serS"
                     /locus_tag="PGA33_000582"
     CDS             complement(21486..22772)
                     /gene="serS"
                     /locus_tag="PGA33_000582"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDG6472419.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     misc_binding    complement(22862..23078)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23151..24665)
                     /gene="hutH"
                     /locus_tag="PGA33_000583"
     CDS             complement(23151..24665)
                     /gene="hutH"
                     /locus_tag="PGA33_000583"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HDG6472420.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQSEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYDEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
BASE COUNT         7319 a         4892 c         3721 g         8901 t
ORIGIN      
        1 acatttcatt tagcgacata aaaaatggtt cctcaatact agaagatgta gatgttttaa
       61 tttcaataaa tttttctaca gctttatctg tatttgttgg atcaaaagct actaaatcat
      121 agccatgacc gtgttgagag cctggattat catttaaaat attcctaaac tgttctttct
      181 tatcttcgtc tattttatta tcaattagct cattaaagta atttagcgct aatttttctc
      241 caactttacc ggttaattta ttctctttat ttgatttttc aatttctgaa tcatttttag
      301 tagtctttga tacacctttt ttatattttg gaattattcc tttaggtgct tccacttcct
      361 tgagtgtctt atctttttgt gctgttctaa tttcttcaat ttcgctgtct tcctgtattt
      421 cgtctatgct attgaccaag ctatcatagg atgtttttgt aacttttgaa gctaattcat
      481 taaatagttc taaaaatttc tttaaatcct ctagcatatc ttcttctgtg aatccttcat
      541 tcaaatcata atatttgaat cttattgatc catgagaata tcctgatgga taatcatttt
      601 ttaaatcata agatgaatct ttattttctg cgtaataaaa tcttccagta ttaaattcat
      661 ttgatgtaat atatttattg agttcggaag ataaagttaa tgctctttgt tttgcagcat
      721 ttttatcccg cggaaacata tcacttatct ttgaccatcc ttgattcaaa gataagtata
      781 tgccttctcc ttccggatga aaaagatata ccaaataata tccatccttt gtttcttttg
      841 ttatattctc atcatatatt gaaatccaag gaactttact atagttccca gtagcaacct
      901 tccctacaac tgaatattta tcttctttta tatgcacttt taactgcttg ggtaacttat
      961 catggactaa agttttatat agatcacctt tatcccaatc agatttttta actacattat
     1021 tggtacgttt ctctttaatt aatttaagga cctgcataaa gttgtctatc atttgaaatt
     1081 ccctcctatt ataaaatata ttatgtctca ttttcttcaa tatgtactta tttatatttt
     1141 accgtaattt actatattta gttgcagaaa gaattttctc aaagctagaa ctttgcttca
     1201 ctataagtat tcagtataaa gaatatttcg ctattattta cttgaaatga aagactgcgg
     1261 aggctaacta tgtcaaaaat catgaacctc attacttatg ataagcttct taaaaacata
     1321 acagcaattc acataaacct catatgttct gatacattca aaatcccttt atgaagcggc
     1381 tgaaaaaacc gcatcattta tgatatgctt ctcctcgcat aatcttaaat gctctgtaca
     1441 cttgttcaat taacacaacc cgcatcattt gatgtgggaa tgtcattttg ctgaatgata
     1501 gtgcgtagtt actgcgttgt aagacgtcct tgtgcaggcc gtttgatccg ccaatgacga
     1561 aaacaaagtc gctttgccct tgggtcatgc gttggttcaa ttcttgggcc aatccttcgg
     1621 aagatagcat ctttccttgt atttctaatg taatgactgt ggattgtggt ttgattttgg
     1681 ctagtattcg ttggccttct ttttctttta cttgctcaat ttctttgtca ctcatatttt
     1741 ctggtgcttt ttcgtctgga acttctatga tgtctatctt ggtgtatggg cctaaacgtt
     1801 tttcatattc tgctatggct tgcttccaat atttctcttt tagtttccct acagctaaaa
     1861 tggtgatttt catgtcgttt ggtcctccaa attgttatca actttccagt tatccacaag
     1921 ttattaactt gttcacactg ttccctctta ttataccaat attttttgca gtttttgata
     1981 ttttcctgac atttatgccc gatttttagt gtataacctg tgtatttgtg gataactctg
     2041 tgggcattgt gggtatttct ctttaaattg tacacaattc tggataacta tccccatttc
     2101 tgtggataaa caccttgatg tcttattact tatcctcgtt tttatatccc atatttatca
     2161 acggttatcg ctttttttca taaaaaaaca ctaccgtacc tcttatttaa gaaatacagt
     2221 agtattattt tcaatatatt ttagctagct tttctacgtc gtacagtcgc gattaaacct
     2281 atacctgcta ataatacaaa catcgcttct gggcttgaac tttgattagt tccagttttt
     2341 ggtaactgtt tctcatgcgc gctaccttta ggcactgaca ttctagccaa ttgtttccca
     2401 ctcttgcttg atacagtagc tccttctatt gtgcgaccag aaccttctgt accgctacta
     2461 acagttcctc tatgcgctag caacaataca actttacctg gagctggttt tttcgctgga
     2521 tccatcactt tgtcgtcacc aattggttgt gtatctttac cagacttact acctttgcta
     2581 ccttgttgtc ctttagctgg ttgttcactt actgctggtt tacctaataa catacgttgt
     2641 ggttctgtcg tatcatactt agctaagtta gctgttttta aataacttgc tagtacttga
     2701 tctaatgaaa taccttcttc tctaggacca ccgaacatac tatatccgtc gccacctgat
     2761 gctgtgaagt cattcatcgt tacgtgatat acacgtttta aatcaatatt ttcaaactta
     2821 cctgtctctt tatttaaaat ttgaatagca ttaattcgtt tgccagacgg tttatttata
     2881 tcatagtaaa cacggattga atcagagata tgtagtaaac cgccattcgc tgttaacact
     2941 gtcttaccgt ccttttgtgt tgttggtgcg cctaaactat gttcgaaagc cgtccagacg
     3001 tctgaacctt ttacatcaat ttgcgcaatc gtatttccaa atggtaatac tgagattaaa
     3061 tcatagcgtg tcaccttacc ttttgcgata gaggcacgaa tacctccacc atttgtcacg
     3121 gcaaagtcag tctttttaga gaaattctta acgccatacg cttccatagc atctgcaatc
     3181 gcgtttccta aatttgtttc acgcgttcta acgtcatctc tttctccttt gaaatcaatg
     3241 gtattgtttg gaataattac ctctgcagtt tgtgctctaa atgtttgatc agcttgatta
     3301 atttgttcag ctaatgcttt gttcggtgtt acattttcaa cgtctttaac attaatcaat
     3361 gacggtttaa tattcgatac ctctccattg cgataattaa atgtaatctt accgatattc
     3421 gcaagtgctg tacctgtttg tgccaatgca tcattgttat aaatttgacc attttgaagt
     3481 actgtatgtg aatgaccatc aataactgta atacgtttct tcaattgtgg attttgactt
     3541 aattgtttca ctaagtaatc accacgccat gtttcttgtg ttgaaggatc aattcctaaa
     3601 tgtgatataa caacaaatgt atctacgtct ttataaatac gcatcatttc cgctgtcaca
     3661 ctttgtaatg gatctctaaa ttcaacgcct ttaatgcctt caggtcttgt tttcgtcttt
     3721 gtttctggtg tcgttacacc aataattcca taacgaatac cattttttgt tacaatcgtt
     3781 gaaggcttaa acgcgcgttt tccatcttta taaacgttag tacttagcat cgggaagtct
     3841 aacataccct ctaacttttt caactgatcg tatccaaagt caaattcatg gttaccgact
     3901 gccatagcat cataacctac tgcattcatt gctttagcca tttcttcacc tttagactgg
     3961 tttgaaagtg gtaaaccttg gaaggcgtct cctgcgtcta acattaaatc aggcttttct
     4021 tgttctttta ctgtttttaa tttagccata ccgatgacac gccctttttc ttcggctagt
     4081 cggccatgga tatcatttgt atgtaatatt ttatgtgttg ttgtttgtgg tgcattagca
     4141 gcaaacattc gtggtgaaag tgatgctgtt ttagcatttg ataatttgaa cgttgctgtg
     4201 tgagttggtt tttgtgttga ggcttgttgt gtatctacgt cgcgtgtttc gtttactttt
     4261 gttgaaacta ctgtagatgg tttgttagat ggtgcgttgg atgttacttt gctgtttgta
     4321 tcatcagctg ttccctgcgc tgatgctgat acgttggttg ccgcttcttt gccagaatga
     4381 tgagccgctt cctttgttgg cggtacttgt tgcttatctg ttgttgcttt gtctatcgtt
     4441 gttactgcat gtgctttcat tggtgtatgc tgctcagccg cgttcgagac ttgccataat
     4501 cccattacac taaaaagcaa aacgagccat acacttgttt taagtaataa agctttcatt
     4561 tcgttccctc cattaactgt catacaccaa tatattatca taatccttat attaaaatga
     4621 ttgattttat gtaaattatt tataaattac atttaatatc gaattatttt acacatttca
     4681 atcaagccga ttcaactcaa caacagccat ttcccaccaa caacataaaa aagcacccga
     4741 taaagtcgtc tcacagcaat gcggaacttt atcggatgac tctcatttat attgtatata
     4801 ttggcgttgg aatagcttta tccgtatcac atagcaatac ttctttttcc gtatcaatat
     4861 cgtgttcgtt caatacttgg ccaacactca tacgcgccaa atctttcatg ttattgtctt
     4921 gtgataaatg cgataggtaa atacgtttcg tgttacctgt aatcacgtct gtcattgcat
     4981 gagccgcatc ctcattagat acatgaccca tatcgcctaa aatacgttgt ttcgtcttcc
     5041 atggataacg acacattctc aacatatcga cgtcatgatt actctcaaaa ataaacgcat
     5101 cgctgccacg tatcatacct ttcatacgat cagacacgta acccgtatcc gttaaaatcg
     5161 taaacttctt atagttatta tggaaaatat aaaattgcgg atctattgca tcatgtgaca
     5221 cgttaaacga ttcaacatcg aaacctgcaa tagattttgt ttcataagga ttaaaaatga
     5281 atttctgatc catagggatg cgactatctt tcttttcaat tgcctgccaa gtcttttcat
     5341 tcgcataaat tggcaattga tatttacgcg ccaaaacacc taatccttta atatgatcaa
     5401 tatgttcatg ggttactaaa ataccattta aatcttgaat attacggtca atttgactaa
     5461 acaattcttc cattttcttt ccagtcaaac caacatcaac taatagacta cctttttcat
     5521 tttctacaaa agtggcgtta cctgtactac cacttgctaa tacactcatg cgtatcaagc
     5581 ggctcatcct ttctctttaa acagtcaatc tctctatatg actgcaatat acatcagtgc
     5641 aaatatacga ttttccctac agaaactatc agtatattag ctgttttgta tacctttcct
     5701 attatgtaac tttagttcat cgaccggttt cttatattta aattttgata atcacgtgca
     5761 atgaatttct taattattat aacaatgatt agacaaatac taaagttttt aaaataacag
     5821 ttacactaat tttacaatgc ggttctactt aaaatatgaa taaatgcgca cgttggagac
     5881 gcaaaaacat cagccataat aaaccgtacc agcaaaaaag ctagcaccgc ttctcacata
     5941 tgatgagctt gcaatgctag cttattacga ttcatattaa tgattaataa ttttagggtt
     6001 attatttgtc gcttcgacat agtaagtatt cgttttatcc ttaccgtcat gcttcacttt
     6061 aatttcccag tttggttgta acaattgaac attcgtttca ttcacgactg agtagtaacc
     6121 gagtctagca ttaatgactt catcaccttt tttcaagtaa cgattataat ataatgcctc
     6181 gattgctttt ctcacaccaa tcacttgatg cttcttatct gcaccatctg tgggcttaat
     6241 gtcatccatc attgattgtt tataactagt cgctttgtta tcttctatat taaagttcaa
     6301 catcgcttta ctgttattta aaatcggaaa atcatcatac gtttgttcat attttacaga
     6361 atctgaacta atctcgctta attgatattc agcaccttta aatacgcgct tgttaacata
     6421 atctttcaaa tcttttaagt tattgtcact gacctttacc gattgactaa tgttcgcatt
     6481 caataatttt ccaccatctg atgtggtcaa atccgaatcg cctttagctt tagaactaaa
     6541 gtcttttgat cgccctgtaa tttgctctaa ttttatacct ttaactgatt tattcaatat
     6601 actagtcggt actttaattt cttcttgctg aaaattaact tcattgttac tctcgacttc
     6661 attaatgtgt gagcgattga ctttattaac ataaatcgat actaacacga tgttgacaag
     6721 aataaacacg aaaatgaaaa gtgtctttgt cagtttccag ttcatttatt caagcctccc
     6781 atcgttataa acataccatt cgccatcata ttctacatac caacgcggta cgagttcact
     6841 gttaatctgc acttcaatgt gattatgatc tgaattatcc tgcatttcgt aaccgatagc
     6901 gatgtttgtt actttttcaa aattaatatc actattgttc gctaagcttg aacgtacaga
     6961 ctctaatttc ggcaacgatt tattatcctc actatttaaa acaacgtcgg tgcgtaataa
     7021 cgaacgacga tagtcaaaga cgcctttttc accccaagtg acttgaattt gattagaacc
     7081 ttctttatta aacgttggat aaccattaag gaaacgttga tatgttaact cgcctgactg
     7141 attattcgta ctaaacaatc taaagtcttc gtttaagaaa ccaccatgac cattaataaa
     7201 atcaaaggtt cctggaatcg tttcttccat tttgctggaa ctcgcttcat cttcggacag
     7261 gtttttataa tgatattttt catttttatc gttatagttt gcgacacctg tattattgtt
     7321 gtaggttgta acaccactct ttgaactacg aacgatggtt gaatcgtcaa atagtatagc
     7381 attcattttc tcaacactaa tcgtgttaaa taccatgcga tatgttttta acttttcagg
     7441 tttacttggt gcaaaaacat gcgtcgtacg atcaattgta tctttgtttg tgatgatatc
     7501 tgtgtatggt tgcatatctt ttttcactgc tgctaatgca tctaaaaaat gatcattttt
     7561 cgttgtagtt gttaatttta cgtaatcgtg gcgatctttg cttatagcat aaagcacgat
     7621 attatcatca gcatcatgat ctatgaccaa acgattgaaa ttgaaatgat ttggtacttt
     7681 cgcattcatg ttcagtactt gaccaagata tgttgataac ggtaaatcat acgtaaaatc
     7741 gaataatata aaatcactgt tcaaatcagg aatcatcaag ttatgatcac gacgaacatg
     7801 ttccacggac ttcacttctt tatttttcaa aggtttcgtc agtttattca cattagatac
     7861 cgtcgcaatc gttccttctg gatgatcatt tttcgaatga ataatctgaa atggcgtaat
     7921 agttgtatcc attttggctg tcataggtgt cgttaaaggt ttcgtttcac tcttcttact
     7981 atctgtattg tcgacatttg caatatcagg agaaaagttc cataccatat atgtcaatac
     8041 gacactcatc aagacgagta gtgctaaaat gacagattta atatgctcct tattattcat
     8101 cccaatcacc gtcttcaatg acttcacatg gaagtgtgat aaagatagat gtaccttgac
     8161 cttctacact gtttgcccaa atacgaccat tgtgcgcttc cacaatctct ttcgaaatgg
     8221 ctagtcctaa tccagtacca cccattttac gcgtacgtgc cttatctaca cgatagaatc
     8281 ggtcgaatat cttatcgact ttattgatag gaataccaat gccattatct ttaatacgaa
     8341 tcgtcattcg attataaagt ggattttgtt tcacgtggaa ctcgacacgt ttatcgcctc
     8401 tagaatattt catcgcattt gtaatgacat tatcaaatac ttgcgtcatt ttatcaggat
     8461 caaattctgt gaaaatcgtc tttttcggaa tatctcgaat aaatgttgta tctttcgcag
     8521 acatttcatg tcgattaata attttattaa tgaacatgtt aaagtcgata atttctttgt
     8581 tgatttgatc agactcatta tccattttag ataactgtag caagtcattg accagtcgaa
     8641 tcattcgttc tgtttcttca cgggtaacag ataaaaattg tggcgcaagt tcctcatctt
     8701 tccatgcacc ttcttcaagt gcttcaatgt aactattcat agaagttaaa ggtgtacgta
     8761 actcatgtga tacattggca acaaattcac gacgctcacg ttcaacttgt tgttgttcag
     8821 ttacgtcatg taacacagcg atataaccag ttacaaatcc tgtttcctgc acaatcgtac
     8881 taaagttaac acgtgcgatt agaccttctt cttcatttaa atctaataag aaactatcat
     8941 tattctcttg aatttcttcc agtttaaatt catcttcaag acttaataca cttaacatgt
     9001 aatatccgat gatgtcttct ttcgccatac caagcatctt gagtgccata tcattgacga
     9061 tacgaatacg tccacggcgg tctgttgcaa taataccatc actcatatgg gtgataactg
     9121 agtccagtct acgtttctca ctttcagtat tagcctgcgc ttcttgtaca cgtttagaca
     9181 agttattaaa tgctaaagct aattcgccaa tttcatcatt accataaatc ttcacacgtt
     9241 gcgtatagtt acctctggac atttcgaccg tctggttacg catatcggtg attggtttgg
     9301 taatcgttcg cgctataaag aatcctagga tgactgtgat taataatgaa atagctgtac
     9361 caacaatgaa tatttgattt atattattta attggttata aacgtcatta atttttgatt
     9421 cgatataaat attaccaatt acctttttat cgactttaac tgggatatta tatacccaga
     9481 cacggtcctt accaccgcca taatctttta aaattaaatg atcgtttgat tgtcctagtg
     9541 atagtgcttt ttggacagaa ctatcattcg ctttttgatt gattagacta cggttagact
     9601 gcttcgtcgt cgcaataata atttggtctt tatctataaa acgaatttct ccaatttctt
     9661 gacggttggc atactcactt aataaatttt gaatatcttt ttgtgcattt acggagccct
     9721 tttcgtcata tactttttca atactaattt ctaattgttt cgcgtactgc gtaatattct
     9781 tcttaaaatt atcaagcagc tctttttcaa ggttatttgt aaaatataac ccgataattt
     9841 gcataccaat gataatcagt aatacataaa caattacaag tttagtatga agggattgta
     9901 gttgttttag ccacttcatt cgtttcgacc tctactcatg ttgttggagg aaatatccaa
     9961 cgcctctacg cgtcacaata tattcaggat gtgacggatc atcttcaatc ttttcacgta
    10021 aacgacgaat cgttacatcg accgtacgta catcgccaaa gtaatcatag ccccatactg
    10081 tttgtaataa atgttcacgt gtcattactt gtcccatatg ttttgataaa taatggaaca
    10141 attcaaattc acgatgtgtt aattcaatat cttcgccacg ttttttaata gaatatgcgt
    10201 ctggataaat cacaatatct ttaattgtga tttcattcgt tacatttcca gtgtcttgtg
    10261 ctggttgtga gtaatgacga cgtaagttcg ctttcacacg tgcgattaat tcacgcgtac
    10321 taaacggttt cgttacatag tcatctgcac ctagttctaa accaagcact ttatcaattt
    10381 ctgaatcttt agcagtaagc attattattg gcatttcgta ttttttgcgc acttcacgac
    10441 atacttccat accatcacga ccaggtaaca tgatatctag taatacgatg tctggttctt
    10501 cttcataaat taagtcgact gcatcattac catcgtatgc acagtacaca tcgtatcctt
    10561 ctttttttaa gttaaattct aaaatatcag caatcggttt ttcatcatca actacaacaa
    10621 cttttctagc catttgcata aacctctttt cttaaatcat taatattatt ttatcatttt
    10681 ttgttcttta tcgtttatgt ttgtgtaaaa aatcacagga tgtcgcttac tatttttgtt
    10741 attgtgcgca ttttccgtct tataacgtat agtaacctat tatataattt accatttata
    10801 agctattaat tctattttcc aatataaact tatatattac tattttttat tagtttctca
    10861 cggcggattg tgacaatgat atttaacaca atgatgaggt ctctattcca ataataaaat
    10921 taggataatg gcaacttcta gttgcacgtc attgctcaga aaataaatat cattattcgt
    10981 tattcgttac tttaacaatt gatgttatgg aggactggtg gcatatcaca acgaagaaat
    11041 atgttgacag tagcatatgt ttgtacgtct aataaagatt ttattgtcta cgtttatctt
    11101 tcatatccaa ccactaagtt acttgaggtt catacatttt aaagaaaatg gacgaacaga
    11161 taaatcaata tgattatcat tcgtccatat atcttttatt aatataatat taaacgcaaa
    11221 aaaatgccta tcctcttgga taggcatttg tacggtctcg acgggaatcg aacccgcgat
    11281 ctcctgcgtg acaggcaggc gtgttaaccg ctacactacg agacctaaaa aatggtgact
    11341 cctacgggac tcgaacccgt gttaccgccg tgaaagggcg gtgtcttaac cgcttgacca
    11401 aggagccgtt cttaagcaca agatagatta tagcacagcc aatcttgcca tgacaagata
    11461 ttttttacat aattttgcta ttattttttg aagaatgtta taagtgaaaa attgcatttt
    11521 attggcagtc tattcattat aataattacg ctatttttga aatttttcta ttttaggtgc
    11581 tttcatttac acttacgtct actctatttt aatagctatt ttcgacatca gacttcttac
    11641 attcaagtat ttcatcttaa ttttcgttaa atatcatcta atcttctaaa ttatgactag
    11701 caaatatcgc ctttacgtta gtatttttta tctaaataat cgaatcgata cagaagcaag
    11761 cataacaaaa gcctcctacc aataaagata gaaggctcat gtatttataa tcattgtgta
    11821 tgacttatat aaagttctac cacaattctt ttaataggtt tgtttgttct ctatctggac
    11881 caactgagaa gatagaaatt tgtacattac ataattctga aatacgctct aaatatttac
    11941 gtgcattttc aggtaattct tctaaagtac gcacatttgt tacgtcttct gtccaacctg
    12001 gtaactcttc aaagattggt ttacaacgtt ttaattgatc taagtttgct gggtactcag
    12061 taatttcttt accgtctaat tcataagctg tacagatttt cactgtgtct aggcctgtta
    12121 aaacatcgat tgagttaata gataaatctg taataccact tacacgacga gagtgacgta
    12181 atacaactga atcaaaccaa cctacacgac gtggacgtcc tgttgttgta ccgtattcac
    12241 gaccaacctc tctaatatga tgtccatctt catcgaataa ttcagtaggg aatggaccat
    12301 caccaacacg tgatgtataa gctttacata caccaattac ctttgaaacg aatgtaggac
    12361 ctacacctgt accaacagta acgttacctg caattggatt acttgatgta acgaatggat
    12421 atgtaccatg gtcgatatct aacattacac cttgcgcacc ttcgaaaagt accttttcat
    12481 ctgctacaaa tgcatcgtct aagatttttg atgtgtctgt tacaaattct tttagacgtt
    12541 gacctgctgc ataatattct tcaaagatat catcaaatga tggacatgtt tcgttaaaca
    12601 tacctttgaa atatgcttgt ttatattcaa tgtttgattt taataatctt tcgaatgttt
    12661 ctttttcaag taaatctgcc atacgaatac cgatacgttg aactttgtct acatatgctg
    12721 gaccgatacc ttttttagtt gtaccaatct tattgtcacc acgtaaacgt tcttcatatt
    12781 catcttgtgc taagtgatat ggtaaaatca cttgcgcacg attagatata cgtaaattac
    12841 ttgtaggaat gccacgttca tttaatccgt ctaattcttt caatagtgca actggatcaa
    12901 caacgactcc gttaccgatt accgctaatt tgtctttgta aaagatacca gatggtacta
    12961 aatgtaattt atatgtttct ccgccaaatt gaatggtatg gcctgcatta ttaccacctg
    13021 aaaaacgcgc gataacatct gactgttctg ccaagaaatc cgttattttt ccttttcctt
    13081 cgtctcccca ttgtgtccca actactacga ttgatgacat gtgagcacct ccaagttttt
    13141 ctcatttaac catttgatat tctaccaata tcgagaccat aatgcaaata aaaatcgaac
    13201 attgattttc ggccttttta aaagaaaaat cattttaaca aatcgttttc aacgtcctaa
    13261 aaacccttta acagaggcgt tgacagtact aatttatcac tttcaataca caaacttaac
    13321 atatatcaac ccgttcagaa caaaataaaa accgtacaat tttatcatct taatgattat
    13381 tgtacggaaa aactttttta catcatatct gcatgtgcat aatcgatatc ggtaaattta
    13441 ttatattgtt tcataaaatg taacttaact gtgcctgttg gaccgttacg ttgcttagca
    13501 atgataattt caatttcacc gttttcatca ttcgtttgtg gctcgaaacc accatcatcg
    13561 tcatcatctt catcgccgcc acggttatag taatcatcac ggtataagaa tgcaacgata
    13621 tcggcatctt gctcaatcga accagattca cgaatatcac tcatcattgg acgtttatct
    13681 tgtcgttgtt caacaccacg agataactga cttaatgcga taactggaca ttctaattca
    13741 cgggctaatg cttttaatgt acgagagatt tcagaaactt cctgttgtct gttatcggac
    13801 gcacgtgaac cactaccttg aatcaactgt aagtagtcaa tcacaatcat gtctaagcca
    13861 tgttcttgct ttaatcgacg acatttagaa cgtaaatcat taattcgaat acccggtgta
    13921 tcatcaataa aaatcttcgt acgtgataat ttacctaccg ctatagtaaa acgactccaa
    13981 tcttcctcag tcatagtacc cgttcttaag cggtttgagt caacatttcc agaactacaa
    14041 atcatacgtg tggctaactg atcagcaccc atctctagcg agaaaatacc aactgtatac
    14101 atatcttcat gcgttgcaac tttttgtgca atattaagtg cgaacgcagt cttacctaca
    14161 gatggacgcg ctgcaaggat aattaaatca tttcggttga accctgctgt catttggtct
    14221 aaatctcgat atcctgtagg tatacctggt gtttgaccac tattttgatc aagctcttca
    14281 gctgtttcat acacttgtcc taagacgtct cgaatgtctt taaagccatc gctttcacga
    14341 gaagatgata gctctaaaat tcgacgttct gcatcactta aaatcgcatc tagttcaagt
    14401 tcatcattat atccatcatt ggcaatacta tctgcagttt gaatcaatct acgttttaat
    14461 gcatgcttag aaacgatatc agtataatac tgaacatttc gcgtcgttgg tacatttgta
    14521 gataactctg caagatattg cgggccaccc gcttcattca acgtaccttc cgtcgataat
    14581 tgatccatca atgttacaac atcaatttct ttattatctt catttaagtg catcattgca
    14641 cggaaaatat gttgatgggc acccctataa aacgactcag gaagcaaaac ttcctgagta
    14701 gtattaatca attctggatc tataataatt gaacctaaga cagactgttc agcttcattg
    14761 ttatgcggca tttgattttg ctcatacatt ctatccatga atggttacac ctcttatttc
    14821 aatccaactt tattgttcaa ctgtgtgtac gcgaattgta ccttcaactt ctttatctaa
    14881 tttaacaggt acattcgtat atcctaggga atgaattcca tttggtaaat ccattttacg
    14941 tttatcaatt ttaatatcat gttgtgcttt tagtgcttcg gcaatttgtt ttgtacttac
    15001 tgacccaaac aatttaccac cttcaccagt ttttgctgat acttcaactt caatgtttga
    15061 taacgtttct tttaatgctt tagcatcttc aatttcttgt tggcgttctt gttttgcacg
    15121 ttttttctgt aactctaatt gtttaaggtt acctggtgtt gcttctacag cataattctt
    15181 tttcaataag aagttatttg cataacctac tggtacttct ttaacttcac cttttttacc
    15241 tttaccttta acatcttgtg taaaaattac tttcatgcat cttcactcct acttaattgt
    15301 tctgtaattg cttgttgtaa ttgtgctatc gcctcttcga ctgtcacacc tttaagttgt
    15361 gttgccgcat tggttaaatg tccaccgcca ccaagtgctt ccattgttaa ctggacattt
    15421 actgaaccga gtgaacgcgc agatatacca atcagattat cttcacgtct cgcaacaaca
    15481 tatgatgctt caataccttc taaacttaac agttcatctg ctgcttgtgc aactgttact
    15541 ggatgataaa ttttatcgtc tgaaccatgc gcaatggcta tgccattatc ttcaactttt
    15601 acagttcgaa ttaattcaga tcgattaatg taagtatcca catcatcttt taagaaatgt
    15661 tgcgttaaaa tcgtatctgc accatgtgca cgtaaataac tcgctgcatc gaatgttctt
    15721 gatcctgttc gtaatgtaaa gtttcttgta tctacaataa tacctgcata catcactgtt
    15781 gattcaagac gtgttaaacg ttgttctgtt ggttgatatt ccagtaactc tgttaccaat
    15841 tcagctgtcg aacttgcgta tggttccata tatatcaaca atggattaga gatgaagctt
    15901 tcaccacgtc tatgatgatc gataacaact ttacggtttg ctttatttaa gacattttca
    15961 tctaaaacca gttccggttt atgtgtatca acaatcacta cggttgtctt agatgtcatc
    16021 atatcccaag catcatctga tgtaataaat cgctctctta actctggctt tttatctatt
    16081 tcgttcatca cgcgtcgtaa tgttggatca atgtcagtct catttaatac gatgtatgct
    16141 tctaaattat tcatcattgc aaatctagac acaccgattg ctgcaccaat tgcatctaag
    16201 tcaggacgtt tatgtcccat gataatgact ttgtcaccct ctgcaaggat atcttttaac
    16261 gcatgtgaga tcacacgtgc tcttacacga gtacgtttct ccatcgggtc agtcttaccg
    16321 ccatagaaac gcacattacc attaatactt ttaattgcaa cttggtcgcc accgcgtcct
    16381 aatgctaagt ctaggcctga ttgtgataat tcacctaagt cgattaaatt ttcagtacct
    16441 tcaccaacac cgatacttaa tgttaattgg gcacgataac caacactttt ttcacgtaat
    16501 tgactcaaga tatcaaattt agattcttct aagtcagcta atattttttg atttaaatag
    16561 gctacgaatt gatcggaact gtatcttttg aaaaatatat tatactcagt tgcccatcga
    16621 ctaatgacac gcgttaccat tgagttgatt tccgaacgct gcgtatcatt catattttgc
    16681 gtaatctcat cgtagttatc taaaaataat gtcgcaatga ttggtttaga attttcatat
    16741 agttcatttg tttgtacttg ttcagttata tcaaagaaat agaggcagtg atcattctca
    16801 gaataacgta cttggaaatg atactgatta tattctattt caacggattt cactctatct
    16861 aattgcttta aaatgtttgg aaatacttca tttacagatt cagaaatgac attcgcttcc
    16921 atatgatctg tcataaattg gttaacccat tcgatgtgat cattttcatc taaaacaatg
    16981 ataccaattg gtaaatgttt gattgcttta ttatttgttg ttgaaatttg agcactcaaa
    17041 ccatctacat aactatccat tttcattaaa gcttgtctga ataaaatgat gctaacaata
    17101 atcatcacga caagaacgat agatgcaatt agtgctataa gactattaaa gataaaccat
    17161 acacccatta aaacaattgc tgtgatgatc atgatgacaa atggtattag taaagctttc
    17221 ttagtggact gccgattcat tattccacct ctattcactt tttagaatta tttttcatga
    17281 ttcgcttcaa attcaaactt aaatcgataa caccaagtag tcctacaata tgtgtcgtag
    17341 gtgtcagtat tgtaccgata accaatagta aaatcgttac tgcattcggc aaacctttcg
    17401 ctttaccaaa gaaatgaata acacttaaac cttgaatata cattactaat gataacacaa
    17461 gttggaagtt taaaagaatg ctctggaaca cactcggttg acctgtaaat aataaacata
    17521 tgataacaat aatgtatatc cataataaaa taccgctcat ttgccacgcg aaaagtggct
    17581 taaatacagg tgtagcgatt ttaaattttc gtaaaatcgg aaatgtaacg attaagttaa
    17641 ttaagacgat taaaaatgta atgataatga tgaaacctgg taattgaacg gtcgcttgtc
    17701 taaacccttc ttctaatatt tgggtcatat tcgcatcggc accgctcatc gtaatcgctt
    17761 catgtaatgt ttgcttgaaa ggttttacta tgctcgctga tggtggaatc cttccgaatg
    17821 tttgtagtaa cataaaagcg attaatgaaa ttaagctcat cgctactgtt gttacgtata
    17881 atattctttc tttagacgtt ctttctttga gcaattgacc aataattaaa cttgcaatta
    17941 agactaatat gatggcactt aaaacgaaag tattacctaa aacagttgtt ataattactg
    18001 taataagtgc actaatcccg aaagattgta ttgatttatt ccataaaacg atacctggta
    18061 tggttgcaaa taatgcaaat attagtccta aaccaggcac tagatataaa gctaaagcga
    18121 caaataccaa cgcaatcgtt gcaattattg ttgctttagg ttgtattttt gaaaacacat
    18181 aagccactcc catattttta actatagcta ttattttaac ctctttaatg aaaattaaca
    18241 atttatagat tgtatgcttc tatttcattt aattgaataa taactttcat gttttataag
    18301 taattaacat actcatttga atcgcttttg tgtgctttca ttttcaacat gattatttaa
    18361 tcccactaca tagcaatcaa gcttgattta gatttacaat acatttccac tctcatgtac
    18421 tctagatgtt tttgaatatg ataactgtga tttagtggct tcattctttg aaaatatata
    18481 ttattactta cgcttaaaat gctttaaatt taagaaatga tataagttag gtgcccaggt
    18541 actaaagttt agtaggaatc catcatgccc aacattatca ggcacgaaga aatgacgatg
    18601 atatttaaaa cgttcaccta atgcacgaac ttgatcatcc ggatatagca aatcatctat
    18661 gaaccccatc gttaacactt ttgtttctaa atttttaaaa acatgcgtta cgtctgtgcg
    18721 acctcggtca atgttgtgac tatccaatac atctagcagt gtcagataac aattcaaatc
    18781 aaaatgttct ttaaatttat taccttgatg ttgttggtat gcgactactt catccggcgt
    18841 gaaacgttca tcataacttt ttgatgatcg atatgtcaaa aaacctaatt ggcgtgcaat
    18901 acttagacct tccttaccac caagatgaat ggcttgcctt gcaatttcat tgaaagctct
    18961 actataagat gatgttcgac ttgttgcagc aaggataatg gctttatcta cttcaaactg
    19021 ttgattgtag agtagttcca ttgcttgcat acctccaaga cttcccccta ttaaaatatt
    19081 aatcttatca taaccaaggg cttgtatacc tcgttcattc gctctgacta tatctcttaa
    19141 tgttaatttt ttaggaaaat gagggtcgtt taaaggtgaa cttgaaccga aaggactacc
    19201 aataacatca aatgttaaaa attgataatc gtgaatgggt atatatcccc catcaataat
    19261 ttctcgccac caacccggat aatcatctgt tccatatgtt aaatgattgc cagttaatgc
    19321 atgacaaact acaactaatg gttgtccatg ataaccgaca tgctcatatc tcaaacgcaa
    19381 gttatctatg acttccccag attctgtaat aaattcccct aaatttaaag tatctactgt
    19441 gtaatttgtc attgttcttt cctccttaaa caaaaaaact tctcacccta ttgaaaagta
    19501 agaagtcttt atacttatca ttcgagtaac tcgttggttt tagcaccgtg ctataaagtc
    19561 ggttgctgaa gtatcacagg gccaagtccc tcaacttctc acaataagaa atatgaaatt
    19621 gttatgtgtt agttgagatt cagtgatgaa ttacttttat catttaaaat gttgttatca
    19681 ttgtcatgcg ttagccaaat cgcttacgta tacacgattc ccaatcttaa catagacgat
    19741 ttgtatatca gaattttctg attactaaca gtttacctaa gtttaaatat ctgttcaatg
    19801 attttcagtt atttttaaaa gaaaaatcgt aatgctgcca tgataacaat cccactaata
    19861 attgtaatag ttaaactacg cgtgattata gataaaataa ccgtcggaat gagcgcgata
    19921 atgtaaggga tgtttaatgt atacccctca ccatgaggcg tctgttgaat aatgctgtca
    19981 atgacaagtg ccgtaaatag tgtgattggg ataaatgata gccatcgaac cacgacatca
    20041 ggcaattgca cttttgaaat catgataaaa ggtataattc gaattaatag cgttacgata
    20101 ccacacaata aaataagtat taacatgttc atatgagtta tcattgttcc atcatcactc
    20161 ctaacgctgc tgaaattgtg gctgcaatta atattgctag atatgaaggc ataaacatac
    20221 ttagcgataa catcattact atgacggcaa taatgagtac tatgtaaatt cttaatcgcg
    20281 atttagtaat tgattcaaat tgcgcaatgg ccaaaaagat aaacatagcc gtgatagcaa
    20341 aatctaaccc tagcgtttgc ggatttgaga tatattcgcc aaataaagcc ccagctacac
    20401 atgaaattgc ccaaaataaa tatgctgtga tgttaagacc atgcatccaa cgatcattga
    20461 tagcttctcc ttttaaataa ggtgtaatgg cgacgccaaa cgtttcgtca gttactaatg
    20521 aacctaatcc aacacggttc caaaacccat atgtcttgaa gtttggtgca agcgacatac
    20581 ttaaaaggaa cattcttgaa tttacgataa atacagttag tacaatcgct gatataggtg
    20641 tacctgctat aaacaacgcg cacataataa attgcgcagc accggcatat ataacaagac
    20701 ataacaagac aatttctaaa atactaaagt tttgagacga agccacaata ccaaatgaaa
    20761 taccaacacc ggcataaccc aataatgttg ggatacactc ttgcacgcct tgtctaaaac
    20821 ttaaatgtgt tgtcatctca attacctcct ttgccacttt atatttatat aattacgaaa
    20881 aaataatata cctagtctaa tacagttcta taccctaagc aataacatta gacatcagtt
    20941 tgtctgaggt tagacatacc ggagtcttta gtcagcttca tattaacttt ttatttttga
    21001 gaattttcaa ttttttattt aagactacct ccatattttc tatggatttg tagttgtttt
    21061 taagtatcaa ttttataaat ttttatatct gatgatgagt ctgggatatt gattcatgta
    21121 ccactccctt ataatcatcc cctcccccta ccctactcca tcgatataac tcatactaca
    21181 tatcaacgaa atcagtattt tatcgcttcc tttcctatat tggtgatgct caaacttgtt
    21241 acgttttaga ttgttttagt tcatcataat tatcccgtat tgttgctata atgaaatgcg
    21301 ttcaccccat taaaccacaa acttaattta ttgttgttat gtgcattggc tcactattat
    21361 acttttacag cacaaaaaaa gtggcgacag cttcgtcacc actttttaaa atattattta
    21421 aagtatcttg cccttgcttt aagtatacgt agatatatac tttttaaagc ttgtagctaa
    21481 agcctttatt taactggttt tgaaatttgt gttttaccac ccataaatgg tactaatgct
    21541 tctggaattg ttactgttcc atcttcattt tggtaatttt caacaatagc agcaaatgta
    21601 cgtccaactg ctaaaccact accatttaat gtatgtgcta attctggttt agctgctttg
    21661 tcacgcttga agcggatgtt agcacgacgc gcttggaaat ccgtacagtt tgagcatgaa
    21721 ctaatttctt tataatcatt gtagcttggt aaccaaactt ctaaatcata tgttttgctt
    21781 gcactaaatc caatatcacc tgtacataaa ataacacgac ggtatggtaa acctaactct
    21841 tctagaattg cttctgcgtt tgttgtcatt tcttctaaag cattccatga atcttcaggt
    21901 tgttcaaaac gtaccatttc cactttatcg aattgatgta aacgaattaa tcctcttgta
    21961 tctctacctg ctgatcctgc ttcactacgg aaacatgcag attgaccagt gaatttttca
    22021 ggaagtacac ctggttgaat aatttcatta cggtagaaat tcgttaatgg tacttcagca
    22081 gttggaattg tatataatcc ttctttttct actttaaata aatcttcttc aaatttaggt
    22141 aattgacctg taccatacat tgtatctgcg ttcacaagct gtggtaccat catttctgta
    22201 taaccatgtt gtgttgtatg ttttgtaatc atatagttca ttaaagcacg ctctaattgc
    22261 gcaccttcat ttgttaaata tacaaaacgc gcacctgaaa cttttgctgc acgatcaaaa
    22321 tcagccattt tcaattcttc tacaatatcc caatgtgctt tgggttcaaa tgaaaactca
    22381 cgtggtgtac cccacttttt aacttcaacg ttatcttcat cagattcacc ttgaggtaca
    22441 tcatcactta ttaaatttgg aatacgacaa aggatacctg tcattttatt atcaatttca
    22501 tttaattgac tatctttttc tttaatatcg tcacctaatg tgcgcatttc agcaatcaca
    22561 tcatcagcat tttctttatt acgttttttt aatgcgattt cttcgcttac tttattacga
    22621 cgtgctttca tttcttctgt tgcactaatt aatttacgtc gttgctcatc caattctaaa
    22681 atttcatcta caacttttgg atcatctcca cgtaattcaa ttttgctctt aactgtgtca
    22741 ggctcatttc tgaataatct aatgtctaac attaaccttc atcctttccc aaataattat
    22801 catttattat ggaatgacgt acgtctttat tttttagaaa ataaaaaaag accacatccc
    22861 tacaagggac gtggtctacg cgttgccacc ctatttaaca atttaagtta taaagataca
    22921 ctaaacctaa attgcacttc actaaaataa cggttatcac cgattgttct tttaaattaa
    22981 gtaggtagat tcatatatat gttgattctt gttcacacta accacaagct ctctgatatc
    23041 gaacactata tattacttgt cctacgaaca atgtcttatt aagttatttt taatatagca
    23101 aactatattt gctttttcaa gtaacgattt caaacatcac tcatgtcgat ttagtgacat
    23161 gcagtcgttt gataaattga ttgctttaaa tactgtgcaa ccgcttcaat atctttatga
    23221 aattgacgat catgtgtaat ggatggcacg atacttcgaa actcatcata cttgcgacgt
    23281 gtttttggtg ataatccttc aacacctttt aactctgctg cttgtaatgc aataacacat
    23341 tcgattgcca gcacacgtct tgcattttca ataatttgat aaccatgtct agcagctgta
    23401 gttcccatag atacgtgatc ttcttggttc gcagatgaag tgatagaatc aacactcgct
    23461 ggatgcgcta aagttttatt ttcagaaacg agacttgcag cagcatattg cataatcatc
    23521 gcgccacttt gcaatcctgg ctctggacta agaaatgctg gtaaatcacc atttaattga
    23581 ggatttacta gtcgctctag acgacgttcc gatacgtttg ctaattcact tacacctaat
    23641 ttaagatgat ctaatgcaaa agcaataggt tgtccatgga agttaccacc tgaaataaca
    23701 aacgtttcat ttgcttcctc aaatataagt ggattatcat tagccgcatt catttcaaat
    23761 tctaattgct gtttaacata attgaatact tgaaaactcg cgccatggat ttgtggtata
    23821 caacgcaacg tatatgcatc ttgtacacgt atttctgatt gtcgcgtcgt taatgttgat
    23881 ccttctaacc aatcacgcat acgcgctgcc acattaatct gttcttgaaa attacgaact
    23941 gcgtgcacat catgtcgata tgcatctata atgccattaa gagactgatg cgttaatgca
    24001 gcaatccatt cagattggta acctaaatct tctgcttcta tataactaat gacaccttga
    24061 gctgtcatag cttgcgtacc attaatcaat gctaaacctt ctttagcctg aaggttcaaa
    24121 ggttgtctat ttaattctct taatacatcg tcactatcct tttcttcccc tctgtacaat
    24181 actttccctt caccaattaa tgctaatgct aaatgtgata atggcgctaa atctcctgat
    24241 gcaccgagag agccttgctg tgggattatc ggtataatac gttcatttat aaaaaattgt
    24301 aattgtctca ctaattctaa agtggcacct gaatgacctt ttaataatgt attcaatcgt
    24361 aaaatcatca tgactaatgc tacttctttt gaaaatggct cacctagtcc acaggcatgt
    24421 gagcgtatca gattcacttg taattcatta tattgcgtcg ggtctatacg tacatcacta
    24481 aataacccaa aacctgtagt aataccgtaa accgtttcct cattttcaat aatacgttca
    24541 actaccgctc tacttttttt gacacgttct aacgcatcat caataatttc aatctttgat
    24601 tgttgttgta aaaatgattt aatatcctca attgttagtg tttcaccatc taaatataaa
    24661 gtcatatatg ttaccccctt gtttatatta agtaacccat ccttcttgaa gtatacgttt
    24721 tcatttttat tgaaacaatg gttttacgta catttataac ctattatcag agcactattg
    24781 tagtgcgtta aaggatatta agattgttgt aagcatattt aataatttat cta
//
LOCUS       DAMQYT010000017        24529 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMQYT010000017 DAMQYT010000000
VERSION     DAMQYT010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24529
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.017"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(26..496)
                     /locus_tag="PGA33_000584"
     CDS             complement(26..496)
                     /locus_tag="PGA33_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDG6472421.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            563..1180
                     /locus_tag="PGA33_000585"
     CDS             563..1180
                     /locus_tag="PGA33_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDG6472422.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            1173..1835
                     /locus_tag="PGA33_000586"
     CDS             1173..1835
                     /locus_tag="PGA33_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDG6472423.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMINKIARIIQTYRQVD
                     TR"
     gene            complement(1951..2634)
                     /locus_tag="PGA33_000587"
     CDS             complement(1951..2634)
                     /locus_tag="PGA33_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDG6472424.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(2884..3960)
                     /locus_tag="PGA33_000588"
     CDS             complement(2884..3960)
                     /locus_tag="PGA33_000588"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDG6472425.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
     gene            complement(4295..5260)
                     /locus_tag="PGA33_000589"
     CDS             complement(4295..5260)
                     /locus_tag="PGA33_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDG6472426.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
     gene            complement(5474..7111)
                     /locus_tag="PGA33_000590"
     CDS             complement(5474..7111)
                     /locus_tag="PGA33_000590"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDG6472427.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAENRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            complement(7271..8149)
                     /gene="hchA"
                     /locus_tag="PGA33_000591"
     CDS             complement(7271..8149)
                     /gene="hchA"
                     /locus_tag="PGA33_000591"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDG6472428.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            complement(8415..9602)
                     /locus_tag="PGA33_000592"
     CDS             complement(8415..9602)
                     /locus_tag="PGA33_000592"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDG6472429.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWNNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
     gene            9773..10948
                     /locus_tag="PGA33_000593"
     CDS             9773..10948
                     /locus_tag="PGA33_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDG6472430.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTFKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            complement(11230..12414)
                     /gene="tuf"
                     /locus_tag="PGA33_000594"
     CDS             complement(11230..12414)
                     /gene="tuf"
                     /locus_tag="PGA33_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDG6472431.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(12631..14712)
                     /gene="fusA"
                     /locus_tag="PGA33_000595"
     CDS             complement(12631..14712)
                     /gene="fusA"
                     /locus_tag="PGA33_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDG6472432.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            complement(14835..15305)
                     /gene="rpsG"
                     /locus_tag="PGA33_000596"
     CDS             complement(14835..15305)
                     /gene="rpsG"
                     /locus_tag="PGA33_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDG6472433.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            complement(15371..15784)
                     /gene="rpsL"
                     /locus_tag="PGA33_000597"
     CDS             complement(15371..15784)
                     /gene="rpsL"
                     /locus_tag="PGA33_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDG6472434.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            complement(15882..16136)
                     /locus_tag="PGA33_000598"
     CDS             complement(15882..16136)
                     /locus_tag="PGA33_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDG6472435.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            complement(16273..19869)
                     /gene="rpoC"
                     /locus_tag="PGA33_000599"
     CDS             complement(16273..19869)
                     /gene="rpoC"
                     /locus_tag="PGA33_000599"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDG6472436.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIHHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            complement(20033..23584)
                     /gene="rpoB"
                     /locus_tag="PGA33_000600"
     CDS             complement(20033..23584)
                     /gene="rpoB"
                     /locus_tag="PGA33_000600"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDG6472437.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            complement(23799..24407)
                     /locus_tag="PGA33_000601"
     CDS             complement(23799..24407)
                     /locus_tag="PGA33_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDG6472438.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
BASE COUNT         7480 a         4739 c         3774 g         8536 t
ORIGIN      
        1 aattttaatt agtatgtact atcgtctaat tggtggattt cttattggct cttaagtttt
       61 taaaaaatgt tgttaataat gtgctacatg cttctttaag tacaccttta tcaacaattg
      121 cacgatgatt aaaattagat tgttgcaata aattcattaa actgccacta caaccacctt
      181 taggatcatc tgcgccatag acgactcttg gaatgcgact cattacaatt gttcctgcgc
      241 acatgacaca tggttctaag gttacatata atgtgcaacc ttctaaacgc caactaccta
      301 acactttggc tgcacgttca attgcaatat gttcagcatg cgccgttggt tgttgtagtg
      361 tttctcttaa attatgtgct ctagcgataa cttcatcatc tttagtgatg atagcaccta
      421 taggtacttc gcctagttga gctgcttttt tagcttcttc aatcgctaat gtcataaaat
      481 atatatcatt tgtcatttat gtccagatac ctcacttatg gtacaatact caatgtctat
      541 tttaacattt ggagatgtga atatgaacaa accttttata gcaattgaag gtcctattgg
      601 cgtaggtaaa tcttcacttg cacacaaatt aagtcaaact ttagattttt atgaagaaaa
      661 agaaatcatc acagaaaatc catttttatc agacttttat gaagatatct ctaaatggag
      721 ttttcaaact gaaatgttct ttttatgcaa tagatataag caatttcaag atgtaacaca
      781 actaaatcaa ggtgtagtta gtgattatca tatacataaa aataagatat ttgctaaaaa
      841 tactttgagt tctgttgaat ttcagaaatt cagtaaaatt tatgatattt taactgaaga
      901 tatgattatg ccgaatatga ttatcttttt agatgcagac cttgatgtgt taaaatctag
      961 aattgctaaa cgtaaccgta gttttgagca tcaaatagaa gatgaatatc tgttaaagtt
     1021 aaaaaaagat tatcgtgagt attatgagtc cttacaaagt aatggttcaa atgtagtttt
     1081 aatcgataca acttctattg attttcttaa aaatgaacaa gattacgaag atatattaca
     1141 tattatatta cctatgatag gagatattac caatgaataa ttacggtatt ccacaaaatg
     1201 ccattataac cattgcaggt acagttggtg ttggaaaatc aacactaacg caagcacttg
     1261 cagataaatt aaactttaaa acgtcttttg aaaatgtcga acataatcca tatttagata
     1321 aattttacag cgattttgaa cgatggagtt tccatttgca aatttacttc ttagctgaac
     1381 gttttaaaga acaaaagcgt atgtttgaat atggtggtgg ctttgtccaa gatcgatcaa
     1441 tttatgaaga tgttgatatt tttgcaaaaa tgcatgaaga agaaggcaca atgagtaaag
     1501 aagatttcaa aacatattca gacttattta atgccatggt catgacacct tattttccta
     1561 aacctgatgt aatgatttat ttagaatgta actatgatga ggtcattgat cgtattattg
     1621 aacgtggtcg cgaaatggaa attaatacag accctgaata ctggaaaaag ctatttaaac
     1681 gctatgacga ttggattaat agctttaatg catgtccagt tgtacgtatc aatattaatg
     1741 aatatgatat ccataaggac cccgaatctt taaatcctat gataaacaaa attgctcgaa
     1801 ttattcaaac atatcgacaa gtagatacac gataaaagac taaagacata gcgtatatgt
     1861 ttatattcaa tgtatattcc atagatatta tcgattattt tatcaattct atcgaataca
     1921 ttaattcaca tatacactat gtctttcttt ttaatttaaa gcttctaaaa tatctgccgc
     1981 actatttaaa ataatatcag cttcatgtaa ttcttctttt gttgcaatac ctgttaatac
     2041 acctattgcc atacctaaat ttgcattact tgctgtcttc atatcattag cagtgtctcc
     2101 tactatagct actttctgag gatctacatt atattgctca aataaaggcg ataatacttt
     2161 aggatttggc ttctcatagg catccgcttc ggtagaaatg atcaaatcga acaacgaggt
     2221 agcattggta tgtgctaaaa attgttctac acctttttta gtatcactcg taacaatacc
     2281 aagttgatag ccttttgctt tcaaatcgat aagtgcttct ttaacacctt ctacccaatt
     2341 aatttcagga atacgttcat ctaccagctt ttgacttgtt gacttggacc agtcggttgt
     2401 atcttgtccc gtcacatcat taaatgcctg gataatttgt tgtaaagatc ctgaacccat
     2461 cactgatttt ggatcaatag attctttaat gacaccgagt tgtcttaaag cagcttcttt
     2521 attatgtact gggaaagtct caagcaatga ttgtacaaat cgtaccccta ttttttccca
     2581 acttctatca aattcaatta acgtaccatc tttatcaaat aatatccatt ccattgatat
     2641 caatactcct atttatttat ttcgtattat gctgattcta tgatattcgt tatcccctga
     2701 aaatgaactc gtagtattgt tctatttaaa tattgaatta aatataataa taagtgaaat
     2761 ccccttcaat acttaacaat aaacattgta aacttaattt attaccatgc ttcgcttcat
     2821 tgaaagggat tttagtcatg attaactttt gcatattgtt ttcatgatta tattcaattt
     2881 ttattaatat tttggtacaa cgactctcca accattttta tcttctaaag taccattttg
     2941 aataccagta tagacgtcgt ataatttttg agtaatttca ccagtctcat tattattaat
     3001 aacgatttca cgatcttcgt atctcaatgt acccacaggt gaaataactg ctgcagtacc
     3061 actaccaaat acttctgtta actcaccttt atcatatgat tcgaataatt catcgattga
     3121 aacgcggcgc tcttcgactt catatcctaa gtttttagct aattcgataa tagatttacg
     3181 tgtaatacca ggtaaaatac tgccattcaa ctctggtgta attactttgc cattttcaac
     3241 gaagaaaatg ttcatgctac caacttcttc gatatatttc tgttcaacac catcaagcca
     3301 taatacttgg tcataaccta atttatttgc attagtttgt gctaataaac ttgccgcata
     3361 gttacctgca acttttgcaa agcctacacc gccacgaaca gcacgcacat attcatcttc
     3421 tacatagatt ttagttggtt ttaaagtttc accaccataa tatgcacctg aaggagataa
     3481 aataattaat aatttatact gatgtgatgc accaacgcca agtgcccctt ctgttgcaaa
     3541 aacaaatgga cgaatatata atgattgacc ttccccttca ggaatccaat ctctttcaat
     3601 atcaactaat tgttttagcc cctctaacaa ttctgcttcg tctacttgag gcatttctaa
     3661 tcgtgctaac gagttattaa gacgcttaaa attttcttca ggacggaaaa gtgcaacttc
     3721 cccatctctt ttatatgctt ttaatccttc gaataccgat tgaccataat gaacaccttg
     3781 tgcagcaggt gaaatttcaa taggaccata aggtactatc ttcaaatcat gccatccttt
     3841 atctgcatca taatcataac tcaacatata atcagtaaaa tatttaccaa aacctagttg
     3901 agatgtattt ggtttttgtt ttaatgtttc tcgtcgttca actttaactg cttgtgacat
     3961 ggtgattgcc tcctaataat attgtataag aatttgttta acttaaatta taacaatcca
     4021 tattttgctg ttcaacaaat tttctaaaaa ttcaaaatta attaacagat ttctagaaag
     4081 actatatctt ttagtataaa cgtattaatt tcacagagac aagtaatctg tgttttacta
     4141 atatacttta catacaaaaa actctttact ttaaaatgaa ctaagctcgc gaattcaata
     4201 agtataatga ataatattag aattcatgca ctagtttatt aaaataaaga gtaatttaaa
     4261 atatcattcc gtgtattaaa gtgaatggaa atgattagtt attattttta acagtatctt
     4321 tttgttcaat agcttctaac attaatttag tcatgctcgc taaatcatat ttaggatcaa
     4381 atccccattc gccacgtgaa cagcttgtat caatagaatc cggccaacta ttagcgatac
     4441 cttgtctaat aggatcaaca tcgtaatcta atgtaaaatt gggatagtat tcttgaattg
     4501 cttcttttac catctctgga tcaaaactca ttgcgctcaa attataacca tttctagttt
     4561 ctaatttagc gtcgtctgct tccataagtt taataattgc ttcaattgca tcatccatat
     4621 acatcatatc catatacgtg cctttatcta tgaagcttgt ataatgaccc tctcttactg
     4681 ctttgaagta tatttcaaca gcatagtctg tagtaccgcc acctggctct ttaacatgcg
     4741 agattaaacc tgggaatcta acacttcttg tatctacacc aaaacgtttg aaatagtatt
     4801 gacacaataa ttctccagct actttattta caccatacat tgtcgtaggt tgctgaatcg
     4861 ttacttgtgg cgtattaact ttaggagttg agtctccaaa tgcaccaatt gaacttggtg
     4921 tgaaaaagtg caaattataa gttcttgcag cttctaatgc attcattaat ccacccatat
     4981 ttaaatccca agctagaatt ggatttttct cagcagttgc tgataataat gctgccatat
     5041 gcattagact atccgcttca aagtccctaa ctaactcaaa catacggtca cgatctgtta
     5101 cgtctaagat ttcaaatggt ccattttgta caggtgagtc tgcttcaggt tccctaatat
     5161 ctgtagcaag aacattatct gtcccataaa tttctctgca cttaacaact aattctgtac
     5221 caatttgtcc taatgcacca gtaatcataa tttttttcat agaaatatct cctttgtctc
     5281 ttctatatag ctatagtcca tcacaagcgg acataatatt cattttcata ataattataa
     5341 tataaaagcg ctttcttgta tatatgacat gtacatgttg ctgatatttc tgtaaatgga
     5401 aattctagtt gtattaattg attttagtaa tttatagcgt ttattattgc taattactgt
     5461 caaattaaat tttttatccc tcaactctta aactctggat atctttcatt atattagctt
     5521 ttttataacc atggatatca tgtaaagcct tataagcttt aaataatgtt tcataccttt
     5581 gtactttttc cgcttctgga ttaatttcgt ataaaattgg ctctttcata gcatcaactg
     5641 cgtcattaat tgaattatgt gcgccaccac tgactgcacc taatattgct gcgcctattg
     5701 ctggtgcata ctcactatcc ataacaatta gttttttatt cagtacattc gcatagatat
     5761 ccatcataac agcattcttt ttaggtatac cgccacatgc aaataccttt tctacttcca
     5821 tatgccaatc ttgatactgt tgcataatca tcttagtacc aaatgctgta gcttctaaat
     5881 atgctctata aatatcctca tgcttagttt gtaaagttaa tccaaagata caacctgtta
     5941 aattgctatc acttaataca cttcgatttc cattatgcca atcaagagca atgagcccac
     6001 tttcacctgg catttgatgt tttatctttt cattcattaa ttcaaataca gtcatatttc
     6061 tattttctgc ttcatctaca tatgactttg gtgcttgctt agcgacatac tcaaacaaat
     6121 cacctactgc tgattgcccc gcttcataag caaataattc tggaataatt gctcctttta
     6181 cagaacctga tatacctggc acttgatgtt gcttttcatt taacataaga tggcatgtgc
     6241 ttgttcccat caccatagtc atttctttat ctttttcaga cccaatacct aataaactag
     6301 catgggcatc aataataaaa ggacttacca tagtttcttt tgataatcct aatttctgtg
     6361 ccattttatc atccagtttc cctactgctt caccaatatt aacaaccggt gcagatactt
     6421 tatcttgaat tacttttgat aatttggggt ctattttatc aaataaatca taatgaaacc
     6481 ctgtttcttc ttcccaaaat gctttgaaac ctaatccaca attcgagcgt acatttttat
     6541 tagttaattt atttacaatc caatcgcccg cttccataat ataagccgtt ttttccataa
     6601 tttctggtgc tcgattcatg acctccatta ttttgggaat catccattca ctactaacat
     6661 tatatccata atggcctaac cacttattat tattttcaat agcagtttga tataatttct
     6721 ctgcctcttt atatgcacca tgatgtttcc aaagtttcac atacgcatgt ggattgtttt
     6781 taaattgttt taagttatgt accgggttaa ggttttcgtc agtaaaaata atagtagatg
     6841 aagtaaagtc tataccaata cctactatat tgtctggatc tatttttgat tcacgtacta
     6901 tatatgatat tccttcttcc ataatttcta aataatcatt actattttga agtgcatatg
     6961 tatgtggtat tttcaaacca tttaattcac tctcaatgac accatgtgta tatggtttca
     7021 caaattttga tactacttga ccgttagttg tattaattaa aaacacacgg cctgaagcag
     7081 ttccataatc aattccaatg ctataagaca tcatgtttcc tccatatcgc tagttgcggt
     7141 tattagtttt gcaagcgttt tcttaaatca tactcataat aggttctatt ttacaagtat
     7201 cacttaaatt ttgccaaaaa aaaagagccc tcatacattt acatatgagg ctcctctaat
     7261 taatatttaa ttatttattt tgtattgcat ttaacatttc attaactgct aattttccta
     7321 actcatttga agctaaagga ctgtcacctg ttaataattt acgatctttt aacgttcttc
     7381 ctgtcatatc gtcgttaact acttttaatc cttgtttagt taataaatca gcaactaacc
     7441 atttcaagcg tccaggtaaa taacctattt caatatttgc accttcatct aatgagtcag
     7501 ggaagacaca aacagagtat ccttctaatg gagatttttc tctgttaagc cctgctgaaa
     7561 gtagtgctgc tggtccatga cataatgtaa ctataaagcg gtcattgtct aatgcccaat
     7621 ctaatgtttg ttgaacgtcc tcactttcag aaataccaac aactgcagca tgtccgcctg
     7681 ggataaagac agataaatag tctgaatcag gtcctaattc atttttaatc acatctgcta
     7741 atttttttgg ctgttttaat ttttctttca atttattata agtacttata actgcctcgt
     7801 cttcagttgg catagcccat aattctaatt taactggata accagataat gtcgcaacat
     7861 caacgtcaaa acctgcttcc attaaatgat gtaaaggtaa taacatttca acaggatgat
     7921 tacccgtaga gaacattttt ccattttcca ataatacata tcgctcttca gcagcaatca
     7981 tcaatacttt ccatttacca tctttatagg cacctttgtg ttcaacacca tcaaaatctg
     8041 ttttaggtgc tgtatattga ctaagggaat atggtgatgg gaaaaatgcg ttatcttctg
     8101 ctttatctgg cgttggttgc ttacttaatt catttacatc ttgtgacata gtttattcct
     8161 actttctcta tatagttact actagtatag acatatacta cacactataa acatgtatta
     8221 ctattaaaat gctcattatc aaaaataatc aaaatgaatt tctttgaaaa aaatacaact
     8281 aaattctaca agatgctttt aaaattcaaa cttcaatttt ggggagcact aaacattgat
     8341 atacgaactt actgttaata aactaaacat taaaaaaagc ccacgacaat atttgccgtg
     8401 ggaataaata aatattaaat caacttcatt tcttttccta ctttttcata agccgcaatt
     8461 gcttcatcta acatgtcttt tgtatgtgca gctgtaggca tatttcttac acgtcctgta
     8521 cctcttggta ctgttgggaa aacgatagat ttcacataga caccttcgtc ttttaaacgc
     8581 ttactaaatt cttgagttgt tttttcatca ccaataatta ctggtgtaat tggagtttct
     8641 gactcacctg tatcatatcc taattttgac aatccatttt ttaaatattg tgcattgttc
     8701 cataatttat catgtaattc agttgaatcc attaactttt taactgcttc agttattgct
     8761 ttggtatccc caggtgctaa tgatgtagag aataagaatg gtcgtgattg tgcttttaac
     8821 caatctatta actcttttgt acctgctaca taaccgccaa cgacaccaat tgctttagaa
     8881 agcgtaccta tttggaaatc gattttatct tgtaaaccaa aatgtttaac cgtaccagcg
     8941 cctttaccca taacacctga accatgagcg tcgtcaacat aagttaataa accaaattct
     9001 tctgcaattt ctacaatttc aggtaattta gccacatcac catccatact aaaaacgcca
     9061 tcagtgatat acatcacttt attgtattga cctgattcaa ctgcttcttt tgctttcgca
     9121 cgtaaatcat ccatgtctga atggttaact cgaataattt tagctttaga taagcgacat
     9181 ccatcaataa ttgatgcatg attaagctca tctgataaaa tagcatcatt tttattcatg
     9241 acagctgaaa tagcagccat attacaatta aatcctgatt gataagctat tgcagcttct
     9301 gttcctttaa attttgctag tgtttcttct aattcgtcgt gtaaatctaa tgtaccattg
     9361 attgtacgaa cagcgcctgc acctacacca tgtgtatcaa tagctgcttt tgcagctgat
     9421 ttcaaatctt catttgttgc tagtcctaaa taattatttg aagataagtt aatgtatgat
     9481 ttcccattga ttttgatttc tggtccgttt gcaccttcaa ttgtatctat ttcattatac
     9541 aaaccatttt cttttagata atttatattt tcctctaaaa actcatgtaa tgattgaacc
     9601 acagtatata atccccctta agtattgttt ggtaatacct ttatcataca gtaattctac
     9661 ataacttgaa agccttatca ttcatagata aaatatctca aagcaatcat ttagtacgat
     9721 aatttcataa aatgttatac tataaacatt attttagaaa gtagtgataa gggtgttaga
     9781 ttggttccaa ctagcaaata ataaagaaaa taaaacaatt caactcagac gttatttaca
     9841 ccaatatcct gagttatctt ttgaagaatt tcaaacacat gattatattg ttaaccaatt
     9901 aagccagtta tcttgtgata ttgaaactcc tatcggccgc aatggtatca aagccacatt
     9961 taaaggcttg ggcacaggac ctaccattgc attaagagct gactttgatg cgttacctgt
    10021 cgaggaatta aacgatgtgc cttataagtc taaaaatcca ggatgtatgc atgcgtgtgg
    10081 gcatgatggt catacagcaa ttttacttac tgtagcagag attttagatg aacataaaca
    10141 tttattagaa ggtaatgttg tattaatatt ccagtacggt gaagaaatca tgccaggtgg
    10201 ttctcaagaa atgatagatg ctggatgtct ggaaaatgta gatagaattt acggtacaca
    10261 tttatggagt ggttatccta ctggaactat tcattcacgt gcgggggcaa tcatggcctc
    10321 tccagatgaa tttagcgtca catttaaagg tcgtggtggt catggtgcca aacctcatga
    10381 aaccattgac ccaatagtaa tcatggcaga gttcatttta agtgcacaaa aaataatttc
    10441 acgtacgatc gatccagtta aacaagctgt actttcattt ggaatgattc aagcaggcac
    10501 cacagatagc gttattcctg atcaagcatt ttgtaaaggt acagttagaa ctttcgattc
    10561 agatatacag aatcatgtaa tggacaaaat ggataagctt ctacaaggtt tagcaatagc
    10621 aaatgatatt aactatgatt tgaattatat taaagggtat ttgcctgtcc ataataacga
    10681 gaaagcatat caagttatta aagaagcaac aaatgactta catgtacgct ttaatgaatc
    10741 agatttaatg atgattggag aagatttttc acattatttg aaagtacgac caggtgcatt
    10801 ctttctgact ggttgtggta atgaaagtaa aggtattaca gcgccacacc ataatcccaa
    10861 atttgatatt gatgaaaaat cattaaaata cgctgtagct gtatttttaa aaatcataga
    10921 attagagcaa gtatttaaaa caaattaagc atttaaatta attgaaagat atgaatctga
    10981 gacataaact aatgaaatgc tcccttcaaa gtagacattg aaaaaatgaa aactttgaag
    11041 ggagcatttt acaatgtcca agactcaaca attttattta aacagtatta tcagttataa
    11101 gttttattag tcagtctttc atcaattatt gttcttctaa atcgaatagc cattacaaaa
    11161 tttacacatt aaaaaaagac ccctcgattg agggatcttc tttttatata aaatctaaga
    11221 aattagaaat tatttaatga tttcagtaac aacgcctgat cctacagtac gtccaccttc
    11281 acggattgag aaacgagtac cgtcttcaat cgcgattgga gcgattaatt ctactgtcat
    11341 ttcaacgtta tcaccaggca ttaccatttc agtaccttct ggtaagtgaa caacaccagt
    11401 tacgtcagta gtacggaaat agaattgtgg acgatagttt gagaagaatg gagtgtgacg
    11461 tccaccttcg tcttttgata atacgtatac ttctgctttg aattcagtat gtggtgtaat
    11521 tgaaccagga gcagctaata cttgaccacg ttgtacgtct tcacgagcaa caccacgtaa
    11581 taatgcacca atgttgtcac cagcttcagc gtagtctaat aatttacgga acatttcaac
    11641 acctgtaaca gttgttttag atgtgtcatg taaaccgatg atttcaactt cttcaccaac
    11701 tttgatttga ccacgttcaa cacggcctgt agcaacagta ccacgaccag tgattgagaa
    11761 tacgtcctca actggcatca tgaatggttt gtcagaatca cgttctggag ttggaatgta
    11821 agtatctaca gcttccatta attctaagat tttttcttcg tattgagcat cgccttctaa
    11881 agcttttaat gctgaaccag cgattacagg tacatcgtca cctgggaagt catattcgct
    11941 taataagtca cgaacttcca tttctactaa ttctaataat tcttcatcgt caaccatgtc
    12001 aactttgttt aagaatacta ctaatgctgg tacaccaacg ttacgtgata aaagaatgtg
    12061 ttcacgagtt tgtggcattg gaccgtcagc agcagatact actaagatac cgccgtccat
    12121 ttgagcagca ccagtgatca tgtttttaac gtagtcagcg tgtcctgggc agtcaacgtg
    12181 agcgtagtga cgtttgtcag tttggtactc aatgtgagaa gtattgattg tgataccacg
    12241 ttctttttct tctggagcgt tgtcaatcat gtcatatgat tgtgcaactg agtcaccatt
    12301 ttttgctaat acagtagcga ttgctgctgt taatgttgtt ttaccatggt caacgtgacc
    12361 gatagtaccg atattggcat gttctttaga acgatcgaat ttttcttttg ccattataaa
    12421 atctctccta ttctaattaa aaataatatt taaatttatc tctcatgata gtttctcacc
    12481 atcatgagaa attatttata gcgatgcaaa aagaatttta caattctttt ataatgcatt
    12541 caccccaaaa aatcaaacta taaagatgat agcttacatt taagctcacc ttgtatttta
    12601 acctaggcta gctagtcaaa acaagttata ttattcacct ttatttttct tgataatatc
    12661 ttcagcgatt gattttggaa cttcagcata gtgatcgaag tacatagtgt aagtaccgcg
    12721 accttgagtg tttgaacgta atgatgttgc ataaccgaac atttctgaaa gtggtacata
    12781 agcattaaca acttgtgcat taccacgagg ttccatacca tcaacacgtc cacgacgaga
    12841 tgttacgtca cccatgatat cacccatgta ctcttcaggc atttcaatag ttactttcat
    12901 cattggttct aagattacag gatcacattt tttagcagct tctttaagtg ctaatgatgc
    12961 agcaattttg aaggccattt cagatgaatc gacatcatgg tatgaaccat catataattt
    13021 agctttaaca tcaattaaag gataacctgc taaaacacca ttttccatag catctttaag
    13081 accagcttct actgatggaa tgtattcacg aggaactaca ccaccaacga tagcgttttc
    13141 gaattcgaaa cctgcgcctg tttcgtttgg tgtgaattca atgtgaacat caccgtattg
    13201 accacgacca ccagattgac gagagaattt accttgaact tgtgcagatg atttgaatgt
    13261 ttcacgatat gaaaccattg gagcacctac gttacattca acgttgaatt ctttcttcat
    13321 acggtctact aagatgtcta agtgaagctc acccatacca ccgatgataa cttgtccagt
    13381 ttcttcgtca gtgtgtgcat ggaatgttgg gtcttcttct tgtaatttaa ctaaagcttg
    13441 agtcatttta tcttggtcag ctttagattt tggctctact gataagtgaa taactggctc
    13501 tgggaattcc attgattcca agataatgtc atttttctca ccacataaag tatcaccagt
    13561 acctgtatct ttaagaccta ccgcagcagc gatatctcca gagtatacag tatcgatttc
    13621 ttgacgtgag ttagcgtgca tttgtaataa acgacctaca cgttcacgtt tacctttagt
    13681 agagttctta acgtatgaac cagatgtcat tgtacctgaa tacacacgga agaatgttaa
    13741 tttaccaaca taagggtcag tcataacttt gaacgctaat gcagcgaatt cagctgaatc
    13801 gtctgctttc gcgattactt cttcttcagg gttgctagca cggtgaccaa taattggttt
    13861 aacgtctagt ggtgaaggta agtaatcaat tacagcgtca agcattaatt gaacaccttt
    13921 gtttttgaaa gctgtaccac aaagtactgg gtagaattct acgttagtag tagcttggcg
    13981 gatagcttct tttaattcag aaactgaaat ttcttcgtca ccaagatatt tttccattaa
    14041 ttcgtcgcta gtttctgcaa ctgcttcgat taagctagca cgagcttctt cagctctatc
    14101 taagtggtct tcgggaattt caatttcttc aatttcagta cctaaatcat ttgtatattt
    14161 gaaacatttc atttcaacta agtcaatgat tgcttcgaat tcgtcttccg caccaattgg
    14221 taattggatt ggagcagcgt tagcttgtaa acgatcatgt aatgtactta cagagtattc
    14281 gaagttagca cctaatttgt ccattttgtt tacaaatacg atacgtggaa caccataagt
    14341 tgtagcctga cgccaaactg tttcagtttg aggttcaaca cctgattgtg catcaagtac
    14401 tgtaactgct ccgtcaagta cacgtaatga acgttcaact tctacagtga agtctacgtg
    14461 tccaggtgta tcgataatgt ttacacggtg accttcccaa gctgctgttg ttgcagcaga
    14521 tgtgatagta ataccacggt cttgttcttg ctccatccag tccatttgtg aagcaccttc
    14581 gtgtgtttca ccaattttgt ggatacggcc agtgtaataa agaatacgtt cagtcgtagt
    14641 cgttttacca gcatcaatgt gagccatgat accgatatta cgagtttttt ctaatgaaaa
    14701 ttctctagcc atgtattttt tctccttcca gtttatatta ctgaataaat acgatagata
    14761 tggcgatgcc ctaagcatga acgcttaatg aaaattcatt aatatagaac acactcaggg
    14821 taaaagcttt tatcttacca acggtagtga gcaaatgctt tgtttgcttc agccattttg
    14881 tgagtgtcct cacgtttctt aacggcacca cctgtattat ttgctgcatc taaaatttcg
    14941 ttagctaaac gatcttccat cgttttttca ccacgaagac gtgcatagtt aactaaccaa
    15001 cgtaaaccta aagtagtacg acgctctgga cgaacttcta ctggtacttg atagttagaa
    15061 ccacctacgc gacgagcttt aacttctaat actggcataa tgttgttgat tgcttcttcg
    15121 aatacttcta atgcatcacg accactgcgt tgttcaacta ggtcgaatgc tgaataaaga
    15181 attctttgtg ctgttccacg tttaccatct aacataattt tgttaattaa tttagttact
    15241 aacttagagt tatgaattgg atctggtaat acgtctcttt taggtactga tcctttacga
    15301 ggcataatgt aaatcctccc ttccttatat taaatatttt aagtttaaga tttaattaaa
    15361 aactaaattc ttagttttta ggtttcttag ttccgtataa tgaacgacct tgtctacgtc
    15421 cgtcaacacc tgaagtatca agtgctccac gtacaatatg gtaacgcaca cctggtaagt
    15481 cttttacacg tccaccacgt acaagtacaa cactgtgttc ttgtaagtta tgtccgatac
    15541 cagggatgta tgcgttaatt tcgatgttgt ttgataaacg cacacgtgca tatttacgta
    15601 acgctgagtt aggtttttta ggtgtcattg tacctacacg agtacataca ccacgttttt
    15661 gtggtgagtt taagtcagta aattttttct ttttactgtt gaaaccttta tttaaagctg
    15721 gagaatctga ttttttgatt ttgctttgtc ttggtttacg tactaattgg ttaatagttg
    15781 gcatgtgata tgtcctcctc tcttcacttt ttaatcccac acatccaggt ggttcatttt
    15841 taggttaaat aaaatttagt aagtaaaaca cttactaatt ctcatttaat caatgctact
    15901 attgtcgcat tgacgttaat acctacatgt ttacccaaag catgtttgct tttgaaaaaa
    15961 gatacaggta tatttttctg attgatttgg cttaacacgc gagtcattaa atatacttca
    16021 acgtcttcag caataatcaa agatgtaact tgatctttct ttaacgcttt aagcgtttct
    16081 ttaagaccaa ctacaaaatg ttgtttgtta aagcgtgcaa ctttttcctt agacaataat
    16141 ttatcctcca aagttctgct tgcatcaacc tttaatatat taacattgaa caagagaatt
    16201 cgtcaacatt aaatcaaata atttacataa aaataacaag aggctaaagc attagcctct
    16261 gttatatact tgttattccg ttacttcagt ttgagattca acttctgcaa ctggtttagc
    16321 tgttttttcg tattttacgt cgctataacg tctcatacca gtaccagctg gaattaactt
    16381 accaataatt acgttttctt taagacctaa taagtcatca cgcttacctt taattgctgc
    16441 atctgtaaga actcttgttg tttcttggaa tgatgctgca gataagaaac tttctgtttc
    16501 aagtgatgct ttagtaatac caagtaatac tggtttagct gttgcaggac gcttacggtg
    16561 tttaaatgct tctctatttg catctgtaaa gttatgaata tcaactaatg aacctggtaa
    16621 taacttcgta tcacctgctt cgataattct aactttacgt aacatttgtc gaaccataac
    16681 ctcaacgtgt ttatcgtcga tttctacacc ttgcatacgg taaacttttt gtacttcttt
    16741 taataagtag ctttcagtcg cgtttaatcc agcaacagat aagtaattct taggttcaat
    16801 agaaccttca gttaatactt caccacgttg aactggttga ccgatttcta caataattct
    16861 tgaagtacct gaagcaaggt atgatcttgt ttcattagca cctttaacaa caatttcttg
    16921 ttgtctatct tttgctaatt taatatcttc tacgacacct tcgatttccg taattaccgc
    16981 ttgaccttta gggttacgtg cttcgaaaat ctcttgaata cgaggaagac cttgtgtgat
    17041 atcgctacct gctaccccac ctgtatggaa tgtacgcatt gtaagctgtg tacctggttc
    17101 accgatagat tgggctgcaa ttgtaccaac tgcttcacca acttcaactt tttcaccagt
    17161 agcaaggttt ttaccgtaac atttttcaca aacaccatgt cgtgcgttac aagtaaatgc
    17221 tgagcgaata tacatttgtt caataccagc atctgtaatt ttcttagcaa tttcaggtgt
    17281 aattaattca tcaggacgaa tgattatttc atcagtttca ggatgatgaa ttgtttcttt
    17341 agaataacga ccttcaatac gttcgataaa tggttcaatc atttctgtac cttctttaat
    17401 atcagaaact aataaacctc tatcagtacc acagtcttct tcacgaacaa taacatcttg
    17461 tgccacgtca acaagacgac gagtaagata tcctgagtca gctgttttaa gtgctgtatc
    17521 ggcaagacct ttacgtgcac cgtgagttga gatgaagtat tcaagtactg ttaaaccttc
    17581 acggaatgaa gatgtgattg gtaattcgat aatcttacca gatggtgcgg ccatcaatcc
    17641 acgcatacct gctaactgtg taaagttaga tgcgttacca cgggcacctg aatcactcat
    17701 catgaagatt gggttagttt tatcaagtga ttgcatcaat tcaccttgaa tttgatcttt
    17761 tgcatctgtc caaatttcaa caactgcatt atatctttct tcttcagtga ttaaaccacg
    17821 gttgaattgt tttgtaattc tgtcgactaa tttttcatgc tcatcaagta tttgttgctt
    17881 atcaggtaat actacgatat cagcaacacc tactgtaata ccagctttag atgagaattt
    17941 gaatcctaag tctttcatac ggtctaacat cattgatgta tcagtgatgc taaatctgtt
    18001 gaatacttct gcaataatat tacctaagaa ttttttgttg aaaggttcaa ttaattcttc
    18061 attttcaaag tattctttta atccaccttc acctaaagtt gtaggatcga tgaaatatct
    18121 gtttggtgtc tttctttcta agttttcttg cgtaggttca ttaatataag caaatgaatc
    18181 tggaatgatt tcattgaata taattttacc tactgacgta gcaagaatct ttttgttttg
    18241 ttcttcagta aatgttggat tattgaacga acttgcatgt acaccaattc tagtgtgtaa
    18301 atgtacaaag ccatttgcat atgcttttaa tacttcattt gtattattaa agattgcgcc
    18361 tgtatttact gcatcttttc tttctaaagt aaggtaatag ttaccaagta ccatatcttg
    18421 tgatggtgta actacaggtt taccatcttt agggttcaag atgttttgtg ctgctaacat
    18481 caacattctt gcttcagctt gtgcctcttt tgataaagga acgtgaaccg ccatttggtc
    18541 accgtcaaag tcagcgttat aagctgttgt tacaagtgga tgtagacgaa tcgcacgacc
    18601 ttcaactaaa gttggttcaa atgcttgaat accaagtcta tgaagtgttg gtgcacggtt
    18661 aagtaataca ggatgttctc taattacttc ttccaatacg tcccaaactt catcatccat
    18721 acgttcgatt ttactcttcg catttttaat gttagttgca atttcacgtt gaactaattc
    18781 tttcattacg aatggtttaa atagttcaag tgccatttct tttggtaaac cacattggta
    18841 cattttcaag cttggaccta ctgcaataac tgaacgtcct gaatagtcaa cacgtttacc
    18901 aagtaagttt tgacggaaac gaccttgttt accttttaac atatgagata aagattttaa
    18961 tggacggtta cctgggccag taactggacg accacgacga ccattatcaa ttaaagcgtc
    19021 aacggcttct tgtaacatac gtttttcgtt ttgaacgatg ataccaggtg cacctaaatc
    19081 taataaacgt ttcaaacgat tatttcgatt aattacacga cggtataagt cgtttaagtc
    19141 acttgttgca aatcgtccac catctaattg aaccattgga cgaatttctg gtgggatgat
    19201 tggaagtaca tctaaaatca tccatgaagg tttgttacct gaattacgga atgattcaac
    19261 aacttctaaa cgtttaattg cacgagtaag tctttgacca gtagctgatt ccaactcatc
    19321 gcgtaacaat ttaagttctt cgtcaagatc aatctcttca agtaaatctt taataccttc
    19381 tgcacccatt tttgcaacga attgacctgg gtatttatca taataatctc tgaattcagc
    19441 ttcagataat aaagttttct tttctaaacc agttggacct ggatctacaa caacataaga
    19501 agcaaagtaa ataacttctt ctaatgctct tggtgacatg tcaagtaata atcccatacg
    19561 acttggtata cctttgaaat accaaatgtg agaaactgga gcagcaagtt caatgtgacc
    19621 cattctttca cgacgtactt tagatttagt tacttcaact ccacatctgt cacagaccat
    19681 gcctttgtag cgaacacgtt tgtatttacc acaactacat tcccagtctt ttgtaggtcc
    19741 gaaaattctt tcacagaata gaccatcttt ttcaggtttt aatgtacggt agttgattgt
    19801 ttcaggtttt ttaacttcac caaaagacca agaacggatt ttttcaggtg aagccaatcc
    19861 tattttcata taatggaaat tatttacatc aatcaaggag cctacctcct tcaatttaga
    19921 ttagctgtgc tatttgattg atttgcattt atttaaaatg gcttaaagta ctaaactgaa
    19981 tcatcaataa aattcagctt agtatctttt tgcctgtttt gtaaattgcg tattaatcag
    20041 taacttcttt ttgtgtttca ggagcatcat tttgttgtaa atctactttg cgttctacaa
    20101 catcatcgtc atcaacgtct gtcatttcga tttcattatc ttgctcatcc ataactttta
    20161 catctaaacc taaactttgt aattctttca tcaatactcg gaatgattct ggaacacttg
    20221 gtctagagat gttttcacct ttaacaatag cctcgtatgt tttcacacgt cctactgtat
    20281 catcggattt gtaagttaag atttcttgta atgtgtatgc agcaccatat gcttcaagtg
    20341 cccatacctc catctcacca aaacgttgtc caccgaattg cgctttaccg ccaagtggtt
    20401 gttgtgtaac aagtgaatat ggtcctgttg aacgcgcatg taatttatca tcaaccatgt
    20461 gcgcaagttt caacatgtac attacaccta ctgaaatacg gttatcgaat ggttcacctg
    20521 tacgtccatc ataaagtaca gttttaccat cacgagccat accagcttct tcaattgttg
    20581 accatacatc gtcatcgttt gcaccgtcaa atactggtga tgcaacgtga ataccaagat
    20641 ttttagcagc catacctaag tgtagctcta atacttgtcc gatgttcata cgagatggta
    20701 caccaagagg atttaacatg atatcgatcg gacgtccatc tggtaagtaa ggcatatctt
    20761 cttcaggaac aatcttagaa atgacacctt tgttaccatg tcgaccacac atcttatcac
    20821 caacatgaat tttacgtttt tgaacgatat atacacgtac taattggttt acaccaggtg
    20881 ataatgtatc gtcgccttct tcacgattga atacttttac atcaagaacg ataccgccag
    20941 cgccgtgagg tacacgtaat gaagtatctc taacttcacg tgctttttca ccaaagattg
    21001 catgtaacaa tctttcttcg gcagttaact cagttacacc tttaggcgtt actttaccaa
    21061 ctaaaatatc tccatctttt acttctgcac caatataaac gataccacga tcgtctaagt
    21121 tcttaagtgc actttcagaa acattaggaa tatctcttgt gatttcttca ggtcctaact
    21181 tagtatcacg tgcttctgat tcatactctt caatatgaat agaagtatac acgtcatctt
    21241 tcacaagtct ttcactcatg ataacggcat cctcatagtt gtaaccgtcc caagtcatga
    21301 aaccaactac tacgtttcta cctaatgcca tttctcctaa ttccatagat ggtccatctg
    21361 ctaaaatctc gttatactca acaacatctc caactgcaac gattggacgt tggttgtaac
    21421 atgtacctga gtttgaacgt ttaaatttag ctaatggata gcgatctaat tcaccttcat
    21481 gctcaacgcc gttctcttca actagacgac gaacaagaat ttcattagat tcaacatgtt
    21541 caacacgacc tctgtgctta gctgtaatag ccgcaccaga atcacgtgct gcaacgtgtt
    21601 ccatacctgt accaacaaat ggtgcttctg gattcatcaa aggcactgct tgacgttgca
    21661 tgttcgcacc catcaatgca cggtttgagt catcattttc taagaatgga atacatgctg
    21721 tcgctgctga aacaacttgc ttcggcgata catccatata atccattttt tctttagcca
    21781 taactgtatt gttaccacgg aaacgacata caacttcatc atccatgaaa cgaccatttt
    21841 catctaattt agagtttgct tgtgctacaa catagctatc ttcttcgtca gctgttaaat
    21901 agtcaatttg atcagtgata gcatgtgtat ctaaatcaac tttacgatat ggtgtttcaa
    21961 taaagccgaa ttcatttaca cgtgcataac ttgataatga gttaatcaat ccaatgtttg
    22021 gtccctcagg tgtttcaatt ggacacatac ggccatagtg agagtagtga acgtcacgta
    22081 cttccatttg agcacgttca cgtgttaaac caccaggtcc taatgctgat agacgacgtt
    22141 tatgcgttaa ctcagctaat gggtttgctt ggtccatgaa ttgtgataat tgagagctac
    22201 caaagaattc tttaatagat gcaataacag gtcgaatatt aattaattgt tgaggtgtga
    22261 tagactcagt atcttgaatt gacattcttt cacgtacaac tctttccatt cttgataaac
    22321 cgatacggaa ttggttttgt agtaattcac ctacagaacg taaacgacgg ttacctaaat
    22381 ggtcaatatc atctgtatat ccaataccgc ttaataagtt aaagaagtaa ctcattgaag
    22441 caatgatatc tgctggtgta atgcatttaa cttctgagtc agggaaagca ttaccaatta
    22501 cagttgtcgt acgaccttca tcatcgttag gaacatatac tttaattgat tgaatttcta
    22561 ctggctcgtc tataacgcta ccatgcaatt caaacacttc gctgtttgca tttgattcaa
    22621 gtacatccat gatttcgtcg attttacgac gatcaagcac tgtaccttct tcaactacaa
    22681 tttcaccagt ttcagtattt acaattggct cagctaattt ttgattaaat aaacgatgtt
    22741 ttaaatgtaa ttttttgttt gttttataac gacccacgct tgctaagtca tagcgttttg
    22801 gatcaaagaa acgtgaatac aatagacttt tagcattttc aacagttggt ggttcacctg
    22861 gacgtaaacg ttcatagatt tctaataacg cttgttcagt gttttcagtg ccgtctttct
    22921 ctaaagtatt acgtaaatat tcattgtcac ctaaaaggtc aacaatttct tggtcgcttg
    22981 agaaacctaa tgcacgtaac aatactgtta atggtagttt acgtgttcta tcaatacgta
    23041 cgtatacaac atctttagca tctgtttcat attctaacca tgcaccacgg tttggaataa
    23101 ttgttgcatc atagttttca cgaccatttt tgtcgatttt ttcattgaaa taaacggatg
    23161 gtgaacgaac taattgagat acgattacac gttctgcacc attgataacg aacgtacctg
    23221 tatcagtcat taatgggaaa tcacccataa agacttcttg ttctttaact tctcctgttt
    23281 ctttaatgat tagacgcact tttacacgaa gaggtgcagc ataagtagcg tcacggtttt
    23341 tagattcttc taaatcatat tttggttctc ctaaacggta atccacaaac tctaatgaca
    23401 aattaccagt aaaatcttca attggagaaa tgtctctaaa catttcgatt aaaccttctc
    23461 ttaggaacca ctcgtaagat ttagtttgaa tttctattaa gtttggtaat tctaatactt
    23521 ctgaaattct cgcgtagttt ctacgtttac gatgtcttcc atattggaca acttgacctg
    23581 ccaaacagat tcacccctca aaaattatgt gatccgcttt tagctaaata ccataagcat
    23641 attaataaaa agacaaaaag aaaacggcaa cactaatgat gacaccattt tcgattctat
    23701 atttattctt aattaactta tctatttaac gtaaaagtac acacttattg aacataaatt
    23761 tttacattat ataactataa cagaatatcc atttcatttc aagcttttat acttctcaga
    23821 atgtaatatc ctttatcttt atttaccact tctacatttc caaaaagttc attcattctt
    23881 ttctttgcag atggcatacc ttgcttcttc tgaattacaa cgaatagttc accgttcgag
    23941 tctaatctat gtaatgcttg ctcgaatata cgatgcacgg tttctttccc tgctcttatt
    24001 ggtggattgg ttaaaataaa atcaaaactt ttgtcttcca cagcagacaa agcatcactt
    24061 tcctttacga tcacattatc aataccattt aattttttgt ttttttcaac taaggctagc
    24121 gctctgtgat taacatctag cattgtaatt gaatgatgtg gtgatacttt agcaatcatc
    24181 aaaccaattg gtccgtaacc acaaccaaca tcggcaattc gcttacttgg accaggtgga
    24241 tgcgctttta aaaaagtttg aacaagaaca tctgaaccat aatctacttt atctttcgaa
    24301 aacactccgt tatcagttat taaatcaatt ttatgatggt ttaattgata ttgaatacgt
    24361 tgttcattgc taattacact tggatcttca tcgtaataat gactcattat tccacctcat
    24421 cttcactttg taactcgtca ttataattat aacatttcac tcagtcttta aatgaataaa
    24481 accccgttat caaaataacg gggttttaac ttctcaaaga atcattata
//
LOCUS       DAMQYT010000018        23915 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMQYT010000018 DAMQYT010000000
VERSION     DAMQYT010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23915
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.018"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            31..219
                     /locus_tag="PGA33_000602"
     CDS             31..219
                     /locus_tag="PGA33_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDG6472439.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANSIFSNEN"
     gene            complement(694..767)
                     /locus_tag="PGA33_000603"
     tRNA            complement(694..767)
                     /locus_tag="PGA33_000603"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(731..733),aa:Arg,seq:tct)
     gene            1131..1265
                     /locus_tag="PGA33_000604"
     CDS             1131..1265
                     /locus_tag="PGA33_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HDG6472440.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            1322..1456
                     /locus_tag="PGA33_000605"
     CDS             1322..1456
                     /locus_tag="PGA33_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HDG6472441.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            1580..2275
                     /locus_tag="PGA33_000606"
     CDS             1580..2275
                     /locus_tag="PGA33_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDG6472442.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDY
                     EINSWKQLNDIVR"
     gene            complement(2384..2823)
                     /locus_tag="PGA33_000607"
                     /pseudo
     CDS             complement(2384..2823)
                     /locus_tag="PGA33_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            3018..4631
                     /gene="bshC"
                     /locus_tag="PGA33_000608"
     CDS             3018..4631
                     /gene="bshC"
                     /locus_tag="PGA33_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HDG6472443.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            4775..5206
                     /gene="mraZ"
                     /locus_tag="PGA33_000609"
     CDS             4775..5206
                     /gene="mraZ"
                     /locus_tag="PGA33_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDG6472444.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            5222..6157
                     /gene="rsmH"
                     /locus_tag="PGA33_000610"
     CDS             5222..6157
                     /gene="rsmH"
                     /locus_tag="PGA33_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDG6472445.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            6171..6572
                     /gene="ftsL"
                     /locus_tag="PGA33_000611"
     CDS             6171..6572
                     /gene="ftsL"
                     /locus_tag="PGA33_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDG6472446.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            6553..8787
                     /locus_tag="PGA33_000612"
     CDS             6553..8787
                     /locus_tag="PGA33_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDG6472447.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            9079..10044
                     /locus_tag="PGA33_000613"
     CDS             9079..10044
                     /locus_tag="PGA33_000613"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDG6472448.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            10046..11395
                     /locus_tag="PGA33_000614"
     CDS             10046..11395
                     /locus_tag="PGA33_000614"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDG6472449.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            11411..12730
                     /locus_tag="PGA33_000615"
     CDS             11411..12730
                     /locus_tag="PGA33_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDG6472450.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEESNDKNNDSASSHANDNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNVISVDNEQPQSAPKEQNSDSIDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEENPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            12836..14248
                     /gene="ftsA"
                     /locus_tag="PGA33_000616"
     CDS             12836..14248
                     /gene="ftsA"
                     /locus_tag="PGA33_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDG6472451.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            14281..15453
                     /gene="ftsZ"
                     /locus_tag="PGA33_000617"
     CDS             14281..15453
                     /gene="ftsZ"
                     /locus_tag="PGA33_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDG6472452.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
     gene            15713..16504
                     /gene="pgeF"
                     /locus_tag="PGA33_000618"
     CDS             15713..16504
                     /gene="pgeF"
                     /locus_tag="PGA33_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDG6472453.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LIYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            16528..17196
                     /locus_tag="PGA33_000619"
     CDS             16528..17196
                     /locus_tag="PGA33_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDG6472454.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            17193..17756
                     /locus_tag="PGA33_000620"
     CDS             17193..17756
                     /locus_tag="PGA33_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDG6472455.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            17768..18058
                     /locus_tag="PGA33_000621"
     CDS             17768..18058
                     /locus_tag="PGA33_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDG6472456.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            18168..18947
                     /locus_tag="PGA33_000622"
     CDS             18168..18947
                     /locus_tag="PGA33_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDG6472457.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            18971..19588
                     /locus_tag="PGA33_000623"
     CDS             18971..19588
                     /locus_tag="PGA33_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDG6472458.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK"
     gene            19809..22562
                     /gene="ileS"
                     /locus_tag="PGA33_000624"
     CDS             19809..22562
                     /gene="ileS"
                     /locus_tag="PGA33_000624"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDG6472459.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            22852..23649
                     /locus_tag="PGA33_000625"
     CDS             22852..23649
                     /locus_tag="PGA33_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDG6472460.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDILGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNNLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
     gene            complement(23710..>23915)
                     /locus_tag="PGA33_000626"
     CDS             complement(23710..>23915)
                     /locus_tag="PGA33_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDG6472461.1"
                     /translation="FTRMSVRGINKVKRELGFVLMAINIRKIAAQRAVHYKIHIKKAD
                     FYQIINRNQLFTLPKNLMSQPPS"
BASE COUNT         8811 a         3293 c         4532 g         7279 t
ORIGIN      
        1 tgattgaaga tttacaaggg ggtgtacaaa atgagaaatc aaattcaaaa actattagac
       61 agtgatttga gcagtttaca tatatcgaaa caaacaggag ttccacaaag cacaatacac
      121 agaatgagaa aaaatgaaag atcattagac aatatgtcat tgaaaaacgc tgaactactt
      181 tataaatttg ccaatagtat atttagcaat gaaaattaag aagaataatt agtgagttaa
      241 ataattaaaa aaccacgtct cgatggacgt ggttattttt tttagatgta tataattata
      301 attataggat ttcgggcccc tgaaaagtcc ctaaaatttt gaatagtatc aaaatctata
      361 ggaataatat aataataaaa atctctacgc atgcaaatgg tgagtgttaa aaatcttggt
      421 gtagtgttgg tgtagttcag gtgcagtata gatgaaattt gagcacaaaa ttacttaatt
      481 aaaaatgtat aaacaagtgt cagacgcgtg tcaattgtgt gtcaaaaaat tctataacga
      541 ataagcatgc ttgttttaat tactgtattt tcggggctta tgcgggggac gttttggtga
      601 cgcatcatac tatattactg aaattcaaaa caaaaagagc cccgtaatca cggaactctt
      661 ttgtttggta atgcgtataa aaatacctat aaacgtcctg ggagggattc gaacccccga
      721 ccgatggctt agaaggccat tgctctatcc agctgagcta ccaggacacg tttaacaaca
      781 caagaattat tatatctaaa tgaacttaaa ttagcaatac cttgtaaata aaaaatgttt
      841 atatttttca ctattataga gctatttatc taaaaaggtt caataagact taaatacgaa
      901 ttcaggcaac ttaattgtgt taaatacagt tttgaatgcc taactgtatt tcttttctct
      961 ttaaaataca gttaagtaca ttataagatg ttgtgcggat aaacaaacta attgtatcaa
     1021 atttatttta aaataacaac aacaaaacga taagcgaata acatttcggt gatttaaaag
     1081 ctacgcacgt ttttgttatc ttcaaattta aattttaagg agtgttttca atggaaggtt
     1141 tatttaacgc aattaaagat accgtaactg cagcaattaa taatgatggc gcaaaattag
     1201 gcacaagcat tgtgagcatc gttgaaaatg gcgtaggttt attaggtaaa ttattcggat
     1261 tctaatttca atatgttatg taagtaatca gtattatttc aaaggtgagg gagagattta
     1321 aatgactgga ctagcagaag caatcgcaaa tactgtgcaa gctgcacaac aacatgatag
     1381 tgtgaaatta ggcacaagta tcgtagacat cgttgctaac ggtgtgggtt tactaggtaa
     1441 attatttgga ttctaatata ataactaata ttctttaaaa taaactgggt gagcatactt
     1501 taatgttatg cactcagttt attttatttg cagaaatttg agcctctgtt aagatttaga
     1561 tacatagaca atataggaga tggggaaatt gggatataaa aatattttga tagactttga
     1621 tgatacaatt gttgattttt atgatgcaga agaatgggcg tttcactata tggcgaatgt
     1681 ttttaatcat aaagcaacaa aggatgattt tttaacattt aaaaaaatca atcaccaaca
     1741 ttgggaagct tttcaacaaa ataaattaac gaagtctgaa gtattatcag aacgatttgt
     1801 gaattacttc aaacatcatc aaatggaagt tgatgggcat cgtgcagatg tgttatttag
     1861 aaatggatta gcagaagcta aagttaaata ctttgatcaa acattagaaa caattgtcga
     1921 attatcgaaa agacatgatt tatatattgt tactaatggt gtaaccgaaa cgcaaaagag
     1981 aaggttaaat cagacgccgt tgcataaata tattaaaaag atatttatat ctgaagaaac
     2041 aggatatcaa aaacctaatc cggaattttt taattatgtt tttaatgata ttggtgagga
     2101 tgaaagacag cactcgatta tagttggaga ttctttaaca tctgacattc taggtggaat
     2161 caatgcgggt atagctactt gctggtttaa ttttagagga tttgatcata atccaggaat
     2221 tatacctgat tatgaaatta attcatggaa acaactaaat gatattgtac gttaagacaa
     2281 ataatttgaa aatgatttac ttatattagc ttaagaaagt aattgtaaaa aagacactac
     2341 gaaaagatgc attttcatgc tcgtagtgtc gaatattatt atcttatggt ttccacaata
     2401 agacatcatg tccctctata tttttagctt ccacatctgt aaatccatga tgtaaaaaga
     2461 aatccttaga atcatttctg ccaatggctt taattggcat attgaaactc tttgcaaaat
     2521 caatcaattc tgaagcgtac cctctgtttt gatattttgg taatacttct aacttccata
     2581 ataatatata atcttcaaaa tctgggaagt agatttcttc gacatcacct tttttagtaa
     2641 tgccattcta gctcctaatt gatctccgac aaatatgcca taaaatggtg aatctgaact
     2701 tgcatcaatc atttgaccgt ttaactcatt gaccatgtat aagtctttgt tgccaaacgc
     2761 tctaaagttt tcgaataatt cgtcagtttt gtaattaatt tcaagacgtt tgatttcact
     2821 catatttatc taccccttat ttgtttacta ctattattat acattaaacc acttgtgttt
     2881 ttcattgatt tgaaaatgta aaacaatgaa tattataaat tttccatgat taaattgatt
     2941 ttcaagccgt aaaattgtca atcaccggta tagactttat actatagttt gtaatcgcgt
     3001 acttaaggag aaaaaacatg gactgtaaag tagttagttt aaatgaaaaa gatcagttta
     3061 taccaaaaat aaagagcagt gaccctgtaa taacaggatt atttcaatat gatgcagctc
     3121 aacaaactag ttttgaaaaa aggatgtcta aagaaaataa tggaagagaa gcggcattag
     3181 cgaatgttat tcgtgaatat atgagtgatt taaagctttc aagtgaacaa gaattaaaca
     3241 tacaacattt agctaatggt tcaaaagttg tgattggtgg acaacaagca gggcttttcg
     3301 ggggaccatt gtatacattc cataaaatat tttcaatcat tactttatct aaggaattaa
     3361 cggatacaca taagcaacaa gtagtaccag ttttttggat tgcaggagaa gatcatgatt
     3421 tcgatgaagt gaatcataca tttgtttata acgaaaatca tgggtcgctg cataaggtta
     3481 aatatcatac aatggagatg ccagagacga ctgtctctag atattatcct gataaggctg
     3541 agttgaaaca aactttaaaa acgatgttca ttcatatgaa agaaactgtt catacacaag
     3601 gtctactgga gatttgtgac agaattattg accaatatga ctcgtggact gatatgttta
     3661 aagcactact gcatgaaaca tttaaagcat atggcgttct atttatagat gcgcagtttg
     3721 agccgttaag aaaaatggaa gcgcctatgt ttaaaaagat tttgaaaaaa catcagttgc
     3781 ttgatgatgc ttttagagca acacaacaac gtactcaaaa tcaaggcttg aatgcgatga
     3841 tacaaacaga tacaaatgtt catttattct tacatgatga aaatatgcgt caattagttt
     3901 cgtatgatgg taagcatttt aaattaaata aaacagataa gacatatata aaggaagaaa
     3961 ttataaatat tgcggaaaat caacctgaat tattttctaa taatgtagtg acaagaccat
     4021 taatggaaga atggttattt aacacggtgg catttgttgg aggaccgagt gaaattaagt
     4081 actgggctga actaaaagat gtatttgaac tatttgatgt tgaaatgcct atcgtgatgc
     4141 caaggcttag aattacttat ttaaatgacc gtatagaaaa attactttcg aaatacaata
     4201 ttccattaga aaaagtgtta gtcgatggtg ttgaaggaga aagaagtaag tttattagag
     4261 aacaagcatc acatcaattt attgaaaagg tagaaggtat gattgaacaa cagcgtcgtc
     4321 taaacaaaga cttattagat gaagtggcgg ggaatcaaaa taatattaac cttgtgaata
     4381 aaaataatga aattcatata caacagtatg attatttgtt aaaacgttat cttttaaaca
     4441 ttgaaagaga aaacgacatc agtatgaagc aatttagaga aattcaagaa acactccatc
     4501 caatgggagg attacaagaa agaatatgga atccacttca aattttgaat gattttggga
     4561 cagatgtgtt caagccctcc acctatccac cactttctta cacttttgat catattatta
     4621 taaaacctta atataccaag ggtttagccc gatttatctt aatgataaat cgggcatttt
     4681 tttgtttttt aaaataaatt tcacaaattt tgtataaata gtggtggata gtggggagat
     4741 gtggtaaatt atatataagg tgaggtgata aaaaatgttc atgggagaat acgatcatca
     4801 attagataca aaaggacgta tgattatacc gtccaagttt cgttatgact taaatgagcg
     4861 ttttattatc acaagaggcc ttgataaatg tttattcggt tacactctag acgaatggca
     4921 acagattgaa gagaaaatga aaaccttacc tatgacaaaa aaagacgcac gtaagtttat
     4981 gcgtatgttc ttctctggtg ctgttgaagt agaacttgat aagcaagggc gtattaacat
     5041 ccctcaaaac ttgaggaaat acgctaattt aactaaagaa tgtacagtaa tcggtgtttc
     5101 aaatcgtatt gagatttggg atagagaaac ttggaatgat ttctatgaag aatctgaaga
     5161 aagtttcgaa gatattgctg aagatttaat agattttgat ttttaaaatg gaggaattga
     5221 agtgtttcat catatcagcg ttatgttaaa cgaaaccatt gattatttaa atgtaaaaga
     5281 aaatggtgtg tacattgact gtacgctagg tggagcggga catgcccttt atttactaaa
     5341 tcaattaaat gacgacggaa gattaatagc aatcgatcaa gaccaaactg caattgataa
     5401 tgctaaagag gtattaaagg atcatttgca taaggtgact tttgttcata gcaacttccg
     5461 tgaattaact caaatattaa aagacttaaa cattgaaaaa gtagatggaa tttattacga
     5521 cttgggtgtt tcaagcccac aactcgacat tccagaacga ggattcagtt atcaccatga
     5581 cgcaacatta gacatgcgta tggaccaaac acaagaacta acagcatatg aaattgttaa
     5641 caattggtca tatgaagcgt tagtgaagat tttttatcgc tatggcgagg agaaattttc
     5701 aaaacagata gctcgaagaa tcgaagcaca tcgcgaacaa caaccaataa caacaacatt
     5761 agaattagtt gacattataa aagaaggtat tcctgcaaaa gcaagaagaa aaggcggaca
     5821 tcctgcaaaa cgagtatttc aagcactacg aattgcagta aacgatgaat tgtcagcttt
     5881 tgaagattca atagaacaag cgattgaatt agtgaaagta gatggcagga tttcggtaat
     5941 cactttccat tctttagaag atcgtttatg taaacaggtg ttccaagaat atgaaaaagg
     6001 tccagaggta ccaagaggat taccagttat accagaagca tatacaccta agttaaagcg
     6061 tgttaatcgt aaaccgatta ccgctacaga agaagattta gatgacaata acagagcacg
     6121 aagcgcgaaa ttacgtgtag ctgaaatact taaataagga gcaatttata atggctgtag
     6181 aaaaagtgta ccaaccatat gacgaacaag tttataatag tataccgaag caacaaccac
     6241 aaactaagcc cgaaaagaag actgtttcga gaaaagtggt tgtacaatta actaaatttg
     6301 aaaaagtttt atacataact ttgattactg taattgctat gttaagtatt tatatgctat
     6361 ctttaaaaat ggatgcgtat gatacgcgag gaaagattgc agatttagat tataaaatag
     6421 ataaacaatc aagtgaaaac agtgctttac aatctgaaat caaaaagaat tcttcttatg
     6481 aacgcatata cgaaaaggct aagaaacagg ggatgagcct tgagaacgat aatgtaaagg
     6541 tagtgcgtag taatggcgaa gcaaaaaatt aaaattaaaa aaaataaaat aggggcagtc
     6601 ctacttgttg gtttattcgg actgctcttt tttatattgg ttttaagaat ttcatatatc
     6661 atgattactg gacattctaa tggtcaagat ttagtcatga aggcaaatga aaagtattta
     6721 gttaagaatg cacaacaacc agaacgagga aagatatatg atcgtaatgg taaagtgcta
     6781 gcagaagatg tagaaagata taaacttgtt gcagtaatag ataaaaaggc gagtgccaat
     6841 tctaaaaaac ctaggcatgt agttgataaa aaagagactg caaagaaatt atctacagtc
     6901 attaatatga agccagagga aattgaaaag agacttagtc aaaagaaagc tttccaaatt
     6961 gaatttggac gcaaaggaac aaatttaacg tatcaggaca aattgaaaat agagaaaatg
     7021 aatttgcctg gtatttcttt attgcctgaa acagaacgct tttatccaaa tggcaatttt
     7081 gcatcacact taattggtag agctcagaaa aatccggata ctggtgaact taaaggtgca
     7141 cttggagttg aaaagatttt tgatagttat ttaagtggat ctaaaggatc attgagatat
     7201 attcatgata tttggggata tatcgcacca aatactaaaa aagagaagca gcctaaacgt
     7261 ggtgatgatg tccatttaac aatcgattca aatattcaag tatttgttga agaagcttta
     7321 gatggcatgg ttgaaagata ccagccgaaa gatttatttg cggttgtcat ggatgccaaa
     7381 actggagaaa ttttagcata cagtcagcga ccaacattta atcctgaaac tggtaaagac
     7441 tttggtaaaa agtgggcaaa tgacctttat caaaacacat acgagcctgg atcaacattt
     7501 aaatcatatg ggttagcagc tgctattcaa gaaggtgctt ttgatcctga taagaaatat
     7561 aaatctggac atagagatat tatgggttca cgtatttcag actggaatag agtcggttgg
     7621 ggtgaaatcc caatgtcact cggatttact tattcatcta atacattgat gatgcattta
     7681 caagatttag ttggtgcaga caaaatgaaa tcttggtatg aacgatttgg atttggaaaa
     7741 tcaactaaag gtatgtttga tggagaagca cctggtcaaa ttggatggag taatgagttg
     7801 caacaaaaaa cgtcatcatt tggtcaatcg acaacagtaa cacctgttca aatgttacaa
     7861 gcgcaatcag cgttctttaa tgatggtaat atgttaaaac catggtttgt gaatagcgtt
     7921 gaaaatcctg ttagtaaaag acaattttat aaagggcaaa aacaaatcgc aggcaaacca
     7981 ataacaaaag atactgctga aaaagttgaa aagcaattgg atttagttgt gaatagtaag
     8041 aagagtcacg ctgcaaacta tcgtattgat ggttatgagg tcgaaggtaa gactggtaca
     8101 gcacaagtcg ctgcacctaa tggtggtgga tacgttaaag gtccaaaccc atattttgta
     8161 agttttatgg gtgacgcgcc gaagaaaaat cctaaagtta ttgtatacgc tggtatgagc
     8221 ttggcacaaa aaaatgacca agaagcttat gaattaggtg ttagtaaagc gtttaaacca
     8281 ataatggaaa atactttgaa atatttaaat gtaggtaaat caaaagatga cacatctaat
     8341 gcagagtata gtaaagtgcc agatgttgaa ggtcaagaca aacaaaaagc tattgataat
     8401 gtgagtgcaa aatcattaga accagttact attggttctg gcacacaaat aaaagcacaa
     8461 tctataaaag cagggaataa agtcttacct catagtaaag tactgttatt aacagatgga
     8521 gacttaacta tgcctgacat gtcaggatgg acgaaagaag atgtcattgc ttttgaaaac
     8581 ctaacaaata ttaaagtaaa tttaaaaggt agcggttttg tgtcccacca atcaattagt
     8641 aagggacaaa aacttactga aaaagataaa atagacgtag aattttcatc agagaatgta
     8701 gacagcaatt cgacgaataa ttctgattca aattcagatg ataagaagaa atctgacagt
     8761 aaaactgaca aggataagtc ggactaacaa gaatggctac attaaattag gatgtattgt
     8821 cactatttgt ttttactaaa gtaatgctgt gatagaaaac aacatcttca tttaatcttt
     8881 ataaataagt gttgatagag aattttaagg tagacgaggt cagattaact tgtttgttat
     8941 aggcaaactt tttatactgg cctcttatgc cgtaaaaaag aaaaattata aattttgatt
     9001 acaactttgt ttatttaatt gaaaaattga taatatatta gtggttaaaa aagtctggat
     9061 taagaagagg agatagttat gatttttgta tatgcgttat tagcgctagt gattacattt
     9121 gttttggtac ctgttttaat acctacatta aaaaggatga aatttggtca aagtattcga
     9181 gaagaaggtc cacaaagcca tatgaagaag actggtacac caacgatggg tggactaaca
     9241 tttctattaa gtattgtgat aacgtctttg gtggctatta tatttgtaga tcaagctaat
     9301 ccaatcatac tgttattatt tgtgacgatt ggttttgggt taattggttt tatagatgat
     9361 tatattattg ttgttaaaaa gaataaccaa ggtttaacaa gtaaacagaa gtttttggcg
     9421 caaattggta ttgcgattat attctttgtt ttaagtaatg tgtttcattt ggtgaatttt
     9481 tctacgagca tacatattcc atttacgaat gtagcaatcc cactatcatt tgcatatgtt
     9541 attttcattg ttttttggca agtaggtttt tctaatgcag taaatttaac agatggttta
     9601 gatggattag caactggact gtcaattatc ggatttacaa tgtatgccat catgagcttt
     9661 gtgttaggag aaacggcaat tggtattttc tgtatcatta tgttgtttgc acttttagga
     9721 tttttaccat ataacattaa ccctgctaaa gtgtttatgg gagatacagg tagcttagct
     9781 ttaggtggta tatttgctac catttcaatc atgcttaatc aggaattatc attaattttt
     9841 ataggtttag tattcgtaat tgaaacatta tctgttatgt tacaagtcgc tagctttaaa
     9901 ttgactggaa agcgtatatt taaaatgagt ccgattcatc atcattttga attgatagga
     9961 tggagcgaat ggaaagtagt tacagtattt tgggctgttg gtctgatttc aggtttaatc
    10021 ggtttatgga ttggagtgca ttaagatgct taattataca gggttagaaa ataaaaatgt
    10081 attagttgtc ggtttggcaa aaagtggtta tgaagcagct aaattattaa gtaaattagg
    10141 tgcgaatgta actgtcaatg atggaaaaga cttatcacaa gatgctcatg caaaagattt
    10201 agaatctatg ggcatttctg ttgtaagtgg aagtcatcca ttaacgttgc ttgataataa
    10261 tccaataatt gttaaaaatc ctggaatacc ttatacagta tctattattg atgaagcagt
    10321 gaaacgaggt ttgaaaattt taacagaagt tgagttaagt tatctaatct ctgaagcacc
    10381 aatcatagct gtaacgggta caaatggtaa aacgacagtt acttctctaa ttggagatat
    10441 gtttaaaaaa agtcgcttaa ctggaagatt atccggcaat attggttatg ttgcatctaa
    10501 agtagcacaa gaagtaaagc ctacagatta tttagttaca gagttgtcgt cattccagtt
    10561 acttggaatc gaaaagtata aaccacacat tgctataatt actaacattt attcggcgca
    10621 tctagattac catgaaaatt tagaaaacta tcaaaatgct aaaaagcaaa tatataaaaa
    10681 tcaaacggaa gaggattatt tgatttgtaa ttatcatcaa agacaagtga tagagtcgga
    10741 agaattaaaa gctaagacat tgtatttctc aactcaacaa gaagttgatg gtatttatat
    10801 taaagatggt tttatcgttt ataaaggtgt tcgtattatt aacactgaag atctagtatt
    10861 gcctggtgaa cataatttag aaaatatatt agcagctgtg cttgcttgta ttttagctgg
    10921 tgtacctatt aaagcaatta ttgatagttt aactacattt tcaggaatag agcatagatt
    10981 gcaatatgtt ggtactaata gaactaataa atattataat gattccaaag caacaaacac
    11041 gctagcaaca cagtttgcct taaattcatt taatcaacca atcatttggt tatgtggtgg
    11101 tttggatcga gggaatgaat ttgacgaact cattccttat atggaaaatg ttcgcgcgat
    11161 ggttgtattc ggacaaacga aagctaagtt tgctaaacta ggtaatagtc aagggaaatc
    11221 ggtcattgaa gcgaacaatg tcgaagacgc tgttgataaa gtacaagata ttatagaacc
    11281 aaatgatgtt gtattattgt cacctgcttg tgcgagttgg gatcaatata gtacttttga
    11341 agagcgtgga gagaaattta ttgaaagatt ccgtgcccat ttaccatctt attaaagggt
    11401 gtgagtattg atggatgata aaacgaagaa cgatcaacaa gaatcaaatg aagataaaga
    11461 tgaattagaa ttatttacga ggaatacatc taagaaaaga cggcaaagaa aaagatcaaa
    11521 ggctacacat ttttctaatc aaaataaaga tgatacatct caacaagctg attttgatga
    11581 agaaatttac ttgataaata aagacttcaa aaaagaagaa agcaatgata aaaataatga
    11641 ttctgcttct agtcatgcga atgataataa tatcgatgat tctacagact ctaatattga
    11701 aaatgaggat tatagatata atcaagaaat tgacgaccaa aatgaatcga atgtaatttc
    11761 agtcgacaac gaacaacctc aatcagctcc taaagaacaa aatagcgact cgattgatga
    11821 ggaaacagta acgaaaaaag aacgaaaaag taaagtaaca caattaaagc cattaacact
    11881 tgaagaaaag cggaagttaa gacgtaagcg acaaaagcga atccaataca gtgttattac
    11941 aatattggta ttgttgattg ctgttatatt aatttacatg ttttcaccac ttagtaaaat
    12001 tgcgcatgta aatataaatg gaaataatca cgttagtact tcaaagataa acaaagtttt
    12061 aggtgttaaa aatgattcaa ggatgtatac gtttagtaaa aaaaatgcta ttaatgatct
    12121 cgaagagaat ccattaatca aaagtgttga gatacacaag caattaccaa acacattaaa
    12181 cgtagatatc acagaaaatg aaattattgc tttagtgaaa tataaaggta aatatttacc
    12241 tttattagaa aatggtaaat tgcttaaagg ttcaaatgat gtcaaaatta atgatgcacc
    12301 tgtcatggat ggtttcaaag gtacaaaaga agatgatatg attaaggcgt tatctgaaat
    12361 gacacctgaa gttagacgat atattgccga agtgacatac gccccaagta aaaacaaaca
    12421 aagcagaatt gaattgttta cgacagatgg acttcaagta atcggtgata tttcgacgat
    12481 atctaagaaa atgaaatatt atccgcagat gtcacaatca ttatcaaggg atagttcggg
    12541 taaactaaaa acacgaggct atattgattt atcagtcggt gcttcattta tcccataccg
    12601 tggaaacacg tctagtcaat cagaaagcga taaaaatgtg actaaatcat ctcaagagga
    12661 aaatcaagca aaagaagaat tacaaagcgt tttaaacaaa attaacaaac aatcaagtaa
    12721 gaataattaa aaaattttga tattgtctat gtttatagtt cacaagccat tcaacgtatt
    12781 gtaaactaag gatagtgtat ttttttaata gtaatttgtc aggaggtgcc tatctatgga
    12841 agaacattac tacgtaagta ttgatattgg atcatcaagc gtaaaaacaa tagtaggcga
    12901 gaaatttcac aatggtataa atgtgatagg tacaggacaa acctacacga gcggtataaa
    12961 aaatggttta attgatgatt ttgatattgc gcgacaagca atcaaagaca caattaaaaa
    13021 ggcatcaatc gcttcgggtg ttgatattaa agaagttttc ctgaaattac ctatcattgg
    13081 aacggaagtt tatgatgaat caaatgaaat cgacttttat gaggatacag aaatcaacgg
    13141 ttcacatatc gaaaaagtat tagaaggtat tagagaaaaa aatgatgtgc aagaaacaga
    13201 agtaattaat gtgttcccga ttcgttttat agtcgataaa gaaaatgagg tttcagaccc
    13261 taaagaatta attgccagac attcattaaa ggttgaagca ggcgtaattg ctattcaaaa
    13321 atcgatttta attaatatga ttaaatgcgt agaagcatgt ggtgttgatg tattagatgt
    13381 ttactctgat gcatataact atggttcaat cctaacagct actgaaaaag agttaggtgc
    13441 atgtgtcatt gatattggtg aagacgttac gcaagttgct ttttatgaac gcggtgaatt
    13501 agtagatgct gattctatcg aaatggcagg gcgtgatatt acagacgata ttgcacaagg
    13561 attaaacact tcttatgaaa ctgctgaaaa agttaaacac caatatggtc atgcattcta
    13621 tgattctgct tcagatcaag atatcttcac tgttgaacag gttgatagtg atgaaacagt
    13681 acagtatact caaaaagatt tgagtgactt tattgaagcg cgtgtagaag aaatattctt
    13741 cgaagtattt gatgttttac aagatttagg attaacaaaa gtaaatggtg ggtttattgt
    13801 aactggtgga tctgcaaact tacttggcgt aaaagaatta ttatcagata tggtaagtga
    13861 aaaagttaga attcacacgc catcacaaat gggaattaga aaacctgaat tttcttcagc
    13921 aatttctaca atttctagta gtatcgcttt tgatgagtta ttagattatg ttacaattaa
    13981 ttatcatgat aatgaagaaa ctgaagaaga tgttattgat gtgaaagaca aagataacga
    14041 atctaaatta ggcggatttg attggtttaa acgtaaaaca aacaaaaaag atactcatga
    14101 aaatgaagta gagtcaacag atgaagaaat ttatcaatca gaagataatc atcaggaaca
    14161 taaacagaat catgaacatg ttcaagacaa agataaagat aaagaagaaa gtaaattcaa
    14221 aaaactaatg aaatctctat ttgaatgatt attggccaat aaaactagga ggaaatttaa
    14281 atgttagaat ttgaacaagg atttaatcat ttagcgactt taaaggtcat tggtgtaggt
    14341 ggtggcggta acaacgccgt aaaccgaatg attgaccacg gaatgaataa tgttgaattt
    14401 atcgctatca acacagacgg tcaagcttta aacttatcta aagctgaatc taaaatccaa
    14461 atcggtgaaa aattaacacg tggtttagga gcaggagcta atcctgaaat cggtaaaaaa
    14521 gctgcagagg aatctcgtga acaaattgaa gatgcaatcc aaggtgcaga catggtattt
    14581 gttacttctg gtatgggtgg cggaactggt actggtgcag caccagtcgt tgctaaaatt
    14641 gcaaaagaaa tgggcgcatt aactgttggt gttgtaactc gtccatttag ttttgaagga
    14701 cgtaaacgtc aaactcaagc tgctgctgga gtagaagcta tgaaagctgc agtagataca
    14761 ttaatcgtta taccaaatga ccgtttatta gatatcgttg acaaatctac gccaatgatg
    14821 gaagcattta aagaagctga caacgtgtta cgccaaggtg tacaaggtat ctcagactta
    14881 atcgctgttt ctggtgaagt aaacttagac tttgcagacg ttaagacaat tatgtctaac
    14941 caaggttctg cattaatggg tattggtgtt tcttctggtg aaaatagagc ggtagaagct
    15001 gctaaaaaag caatctcttc tccattactt gaaacatcta tcgttggtgc acaaggtgtg
    15061 cttatgaata ttactggtgg cgagtcattg tcattatttg aagcacaaga ggctgctgat
    15121 attgtccaag atgctgcaga tgaagacgtt aatatgattt tcggtacagt tattaatcct
    15181 gaattacaag atgagattgt tgtaacagtt attgcaactg gttttgatga caaaccaaca
    15241 tcacatggtc gtaaatctgg tagcactgga ttcggaacaa gcgtaaatac ttctagcaat
    15301 gcaacttcta aagatgaatc attcacttca aattcatcaa atgcacaagc aactgatagt
    15361 gtaagtgaaa gaacacatac aactaaagaa gatgatattc ctagcttcat tagaaataga
    15421 gaagaaagac gttcaagaag aacaagacgt taatcggtta atatatatac acaaataatt
    15481 caacacaaat catcagataa catatctgat gattttttta ctaattttta gaacatgtag
    15541 aaggacattt aagtttttca aagttattaa aagtgtttaa gtatcgtgtg aaaattaagt
    15601 caaaaattat ttgcgcaaca ttttaacttt aaacataaat gttatattat ataattatta
    15661 actttgtaca gttagacgaa gataatttaa atgaaatgat ggtgacgatc gagtgaatga
    15721 taattttaaa aagcaaccgc atcatttaat atatgaagag ttattacaac aaggtattac
    15781 tctaggtatt acaactagag gagatggttt aagtgactat cctaaaaatg cttttaatat
    15841 ggcgagatat attgatgatc gcccatataa tattactcaa catcaattgc aattagctga
    15901 agaaattgcg tttgatagaa aaaattgggt gtttcccatt caaacacatg aaaataaagt
    15961 cgcttgtatt acaaaggatg atataggcac aaatatagac actttaactg atgcgcttca
    16021 tggtattgat gcgatgtaca catatgatag taatgtctta ttaacgatgt gttatgcaga
    16081 ctgtgtacca gtatattttt atagtacaaa acatcatttt attgcattgg cgcatgcagg
    16141 ttggcgtggt acctatactg aaattgtaaa agaagtgcta aaacatgtga actttgattt
    16201 gaaagactta catgtcgtta ttggaccatc tacatcatca agttatgaaa ttaatgatga
    16261 tattaaaaat aaatttgaaa cattgccaat tgatagtgcc aactatattg aaactagagg
    16321 acgagatcgt catggtattg atttgaaaaa agccaatgct gcattattaa tttattatgg
    16381 tgttcctaaa gaaaatattt atacgacagc gtatgctaca tctgaacatt tagaattatt
    16441 tttctcttat cgattagaaa aaggtcaaac aggacgcatg ttagcattca ttggtcaaca
    16501 gtaaacaagg aggagatatg tttgcgtgtg aaagataatt tacaacaaat ctcaacacaa
    16561 attaatgaca aaagtgaaaa aaataatttt tcaacaaaac caaacgtgat tgcagttaca
    16621 aaatatgtta caatagagcg agctaaagaa gcgtatgagg ctggaataag acattttggt
    16681 gagaatagat tggaaggctt ttttcaaaag aaagaagcat taccatcaga tgcggtgatc
    16741 cattttatag gatcattaca atctcgaaaa gttaaggacg ttataaacga cgtagattat
    16801 ttccatgctt tagatcgatt gagcttagcc aaagaaatta acaaacgtgc agaacataaa
    16861 attaaatgtt tcttgcaagt gaacgtttcg ggagaagctt ctaaacatgg tattgcttta
    16921 gaagatgttg atcagtttat agatgatctt aaaaaatatg acaaaatcga aattgtaggt
    16981 ttaatgacga tggcaccatt gacagatgat gaagcatata ttagatcgtt atttaaacag
    17041 ttacgtttga aaaaagaaga aatacaacga ctcaatttag aatatgcgcc ttgtgatgaa
    17101 ttatcaatgg gaatgagtaa tgactatctt attgcagttg aagaaggtgc gacgtttgtt
    17161 agaattggga ctaaacttgt aggagaagag gagtgagcca cttggcttta aaagatttat
    17221 ttagtggatt ttttgtaata gatgatgaag aggaagtaga agtacctgac aaacaacaac
    17281 aggtaaatga agcgccagca aaagagcagt cacaacaaac aacaaaacaa aacgcaatca
    17341 aatcagtccc tcaaaaatct gcatcaagat atacaacaac gtcagaagaa aggaataacc
    17401 gtatgtctaa ttattcaaaa aataattcac gtaatgttgt aactatgaac aatgctacac
    17461 caaacaatgc atcacaagaa agttcaaaaa tgtgtttatt cgaaccacgt gttttttcag
    17521 atacacaaga tattgctgat gagcttaaaa accgccgtgc gacacttgtc aatttacaac
    17581 gtattgataa agtatcagcg aaaagaatta ttgatttttt aagcggtact gtttatgcaa
    17641 tcggtggaga tatccaacgt gtaggtactg atattttctt atgtacgcct gataatgtgg
    17701 aagtagctgg aagcattaca gaccatattg aaaatatgga acattcattc gactaaggag
    17761 ttaacatatg gatataaatg tgctagctac aatatttaaa ttcatccttt ttgttgttga
    17821 aatttattat ttcggcatga ttatatattt ctttacatct tgggtaccaa gtattagaga
    17881 aactaaggta ggttattttt tagcgaaaat atatgaacct ttcttacaac catttagaaa
    17941 agtaattcca cctattggaa ttatcgacat atcatcaatc gctgcaatta tcgttttagt
    18001 attattccaa aaagggttac tccaaatctt taattggatt ttaattcaat tacaataaat
    18061 taagcgacat aacgaggcaa gggtacatga tactttagcc tcgtttttga tatgcatttt
    18121 tctgaatata agggcaatag atggtatttt ataatttttt taaggtagtg attaacatag
    18181 atatttatca acactttaga caggaagaat acgaattaat tgatcagcta acggataaat
    18241 gtgatcaagc ggaacagcat tatgcaccag tattaacgca ttttttagat ccaagagggc
    18301 aatatatatt ggaagtgatt tgtggcagtt atgaagattt aaacgtatct ttttatggtg
    18361 gacctaatgc tgaaagaaaa agagcaatca tttcgccgaa ctattatgaa cctaaagaaa
    18421 gcgactttga attaacttta atggaaatag attatcctga aaaattcgtc actttaaaac
    18481 atcaacatat tttagggaca ttaatgtctt taggtatcga acgcgaacaa gttggagata
    18541 taattgtgaa tgaacgaatt caatttgttt tgacaagtag attggaatca tttattatgt
    18601 tagaattaca acgtattaaa ggcgcatcag ttaaacttta tactattcca gtaacagata
    18661 tgatacaatc taatgagaat tggaaaaatg aaagtgcaac agttagttct ttaaggttag
    18721 atgttgttat taaagaaatg atacgtaaat cacgtacgat tgcgaaacaa ctaatcgaaa
    18781 aaaaacgtgt taaagtgaat cacactattg ttgattcagc agattttcaa ttacaagcaa
    18841 atgatttaat atccatccaa ggttttggta gagcacacat tactgactta ggtggtaaaa
    18901 ctaaaaaaga taaaacgcac attacctata gaacattatt caaatagtaa tgatttaagg
    18961 aggataacaa atgcctttta caccaaatga aattaagaat aaagagtttt cacgtgtaaa
    19021 gaatggttta gaacctactg aagttgctaa ttttttggag caactaagca ctgaaattga
    19081 acgtcttaaa gaagataaaa aacaacttga aaaagtaatc gaagagagag atactaatat
    19141 taagtcttat caagacgtgc atcaatctgt aagtgatgct ttgatacaag ctcaaaaagc
    19201 tggtgaagaa actaagcaag ctgcagagaa acaagctgaa gcgattatag ctaaggcaga
    19261 agcgcaagct aatcaaatgg ttggtgacgc ggtagaaaaa gcacgccgtt tagcattcca
    19321 gactgaagat atgaaacgtc aatcaaaagt atttagatcg cgtttccgta tgttagttga
    19381 agcgcaatta gacttattaa aaaacgaaga ttgggattac ttgttgaatt atgatttaga
    19441 cgctgaacaa gtgacgcttg aaaatattca tcatttgcat gaaaatgatt taaagccaga
    19501 tgaagttgca gcaaatgcac aaaataatgc atcaaataca ccagacaata atcaacaatc
    19561 caatgattca gaaacaacta agaagtaaga attaaataaa gacagacgcg taatatacat
    19621 ttaacttttc acagcgaatt aggtaatggt gagagcctag taaaagcatg tatgttatat
    19681 cactggcttt ttaatattta aataatgtaa tgagagaact ctaagttgag ttaataaggg
    19741 tggtaccgcg agcaatcgtc ccttttaatt taacttagag ttttttaaat ttttaaggag
    19801 tgaaaaaaat ggattacaaa gaaacgttat taatgcctaa aacagatttc ccaatgcgag
    19861 gtggtttacc aaacaaggaa ccgcaaattc aagaaaaatg ggatgcagaa gatcaatacc
    19921 ataaagcgtt agaaaaaaat aaaggtaacg aaacattcat tttacatgat ggcccaccat
    19981 acgcgaatgg taacttacat atgggacatg ccttgaacaa aattttaaaa gactttattg
    20041 tacgttataa aactatgcaa gggttctatg caccatacgt accaggttgg gatacacatg
    20101 gtttaccaat tgaacaagca ttaacgaaaa aaggtgttga ccgaaagaaa atgtcaacag
    20161 ctgaattccg tgagaaatgt aaagaatttg ctttagaaca aattgaatta cagaaaaaag
    20221 attttagacg tttaggtgtt cgtggtgact ttaatgatcc atatattaca ttaaaacctg
    20281 aatacgaagc tgcacaaatt cgtatttttg gagaaatggc agataaaggt ttaatttata
    20341 aaggtaaaaa gccagtttat tggtctcctt caagtgagtc ttcattagca gaagcagaaa
    20401 ttgaatatca cgataaacgt tcagcatcaa tttacgttgc atttgacgtt aaagatgaca
    20461 aaggtgtcgt tgatgcagat gctaaattta ttatctggac aacaacgcca tggacaattc
    20521 catcaaatgt tgcgattacc gttcatcctg aattaaaata tggtcaatac aatgtaaatg
    20581 gcgaaaaata tattattgca gaagccttgt ctgacgctgt agcagaagca ctggattggg
    20641 ataaagcatc aatcaaatta gaaaaagaat acacaggtaa agaattagag tatgttgtag
    20701 cacaacatcc attcttagac agagaatcgt tagtgattaa tggtgatcat gttactacag
    20761 atgctggtac aggttgtgta catacagcac caggtcacgg ggaagatgac tatattgttg
    20821 gtcaaaaata tgaattgcca gtaattagtc caatcgatga taaaggtgta tttactgaag
    20881 aaggcggcca atttgaaggg atgttctatg ataaagctaa taaagccgtt actgatttat
    20941 taacagaaaa aggtgcacta ttaaaattag actttattac acatagctat ccacacgact
    21001 ggagaacaaa aaaacctgta atcttccgtg ctacaccaca atggtttgcc tcaatcagta
    21061 aagtaagaca agatatttta gatgcaatcg aaaatacaaa cttcaaagta aattggggta
    21121 aaacacgtat ttacaatatg gttcgtgacc gtggcgaatg ggttatttct cgtcaacgtg
    21181 tgtggggtgt accgttacca gtattttatg ctgaaaatgg cgaaattatc atgacgaaag
    21241 aaacagtgaa tcatgttgct gatttatttg cagaacacgg ttcaaatatt tggtttgaaa
    21301 gagaagcgaa agacttacta ccagaaggat ttacacatcc aggcagccct aacggtacat
    21361 ttactaaaga aacagacatt atggacgttt ggtttgattc tggttcatca caccgtggcg
    21421 tgttggaaac aagaccggaa ttaagtttcc cagcggatat gtatttagaa ggtagtgacc
    21481 aatatcgtgg ttggttcaac tcttctatca caacttcagt tgctacaaga ggagtatcac
    21541 cttataaatt cttactttct catggttttg ttatggacgg tgaaggtaag aaaatgagta
    21601 aatctttagg taatgtgatt gtacctgacc aagtggttaa acaaaaaggt gctgatattg
    21661 cgagactttg ggtaagtagt acggactatt tagctgatgt tagaatttct gatgaaattt
    21721 taaaacaaac atctgatgtt tatcgtaaaa tcagaaatac attaagattt atgttaggta
    21781 acattaacga tttcaatcct gacacagata gcattcctga atcagagtta ttagaagtgg
    21841 atcgttactt gctaaatcgt ttacgtgaat ttactgcaag tacgattaac aactatgaaa
    21901 actttgacta cttaaatatt tatcaagaag ttcaaaactt tatcaatgtt gagttaagta
    21961 atttctattt ggattacggt aaagatattt tatatattga acaacgtgat tctcatatcc
    22021 gtcgtagtat gcaaacagtg ttatatcaaa ttttagttga tatgacgaag ttgttagcac
    22081 caatcttagt gcatacagct gaagaagttt ggtctcatac accacatgtt aaagaagaaa
    22141 gtgttcactt agcagacatg cctaaagttg tagaagtaga tcaagcttta ttggataaat
    22201 ggcgtacatt tatgaattta cgtgatgatg tgaaccgtgc attagaaact gctcgtaatg
    22261 aaaaagttat tggtaaatca ttagaagcta aagttacgat tgctagtaac gataaattta
    22321 atgcatctga attcttaact tcatttgatg cattacatca attatttatc gtgtcacaag
    22381 ttaaagttgt agataagtta gacgatcagg caacagctta tgaacatggt gatattgtca
    22441 tcgaacatgc agatggtgaa aaatgtgaaa gatgttggaa ctattcagag gatcttggtg
    22501 ctgttgatga attgacgcat ctatgtccac gatgccaaca agttgtaaaa tcacttgtat
    22561 aattgaaatt gtataaagta ctcatacaga tgatataaat taaagctctc ttcataatca
    22621 tgttgtagtt tttgttgaca tgatgaagag agtttttttg tgaataaaaa aatgaccaag
    22681 ttaccggtca tatatgtaaa aaatgtgcga tttactaaaa taaaaattat tcaggaatgg
    22741 tacaaattct ctgaggcata taaatgcgtt atagttgcta ttctcaatta tgttcgcgat
    22801 aattttaagt aaaagtaagc acagatattg aatttgatag gagttaattg aatgtatcat
    22861 aacagtaacg caaactttgt caatggtatc actttaaatg tgagagataa gaatgaatta
    22921 aagccatttt atgaggacat attaggatta aatattataa atgagacatt aacatcgata
    22981 caatatgaag taggtcaaaa taatcatgtc attacacttg ttgaattaca aaatggacgt
    23041 gaacctttaa tgtccgaagc gggactgttt catatcgcaa ttaaactacc tcaaattagt
    23101 gatttagcta atttactaat tcatttaagc gaatatgata ttccagttaa cggaggtata
    23161 cagcctgctt cgttatcatt attttttgaa gacccggaag gaaacggttt taaattttat
    23221 gttgataaag acgaagcgca atggacgagg caaaataatt tagtaaaaat tgatattaga
    23281 ccattaaatg taccgagatt agtgagtcat gcaacaaaat tgttatggtt aggtattcca
    23341 gatgacgcta ttataggtgc attgcatatt aagacaattc atttatcaga ggtaaaagag
    23401 tactacctcg attattttgg attagagcaa tcggcatata tggatgatta ttcaatattt
    23461 ttagcatcga atggctatta tcaacatttg gccatgaatg attgggtatc agcaacgaaa
    23521 cgtgtagaaa attttgatac gtatggatta gcaattgttg actttcatta tcctgaaaca
    23581 acacatttaa atttacaagg tccggatggt atctattatc gctttaatca tatcgaagtt
    23641 gaagattagt atatactttg aatggacgaa ccatataatg aatcgttttt aatgatcttt
    23701 ttatacaagt tatgaaggag gctgggacat taagttctta ggcaatgtaa aaagctgatt
    23761 tctattaatt atttgataga aatcagcttt tttgatatgt attttataat gtacagctcg
    23821 ttgagctgct attttcctta tattaattgc cattaataca aaacctagct ctcgtttaac
    23881 tttatttatt cctcgaactg acattcgagt gaaac
//
LOCUS       DAMQYT010000019        23496 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMQYT010000019 DAMQYT010000000
VERSION     DAMQYT010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23496
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.019"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            28..948
                     /locus_tag="PGA33_000627"
     CDS             28..948
                     /locus_tag="PGA33_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDG6472462.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            complement(1056..1475)
                     /locus_tag="PGA33_000628"
     CDS             complement(1056..1475)
                     /locus_tag="PGA33_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472463.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(1472..1963)
                     /locus_tag="PGA33_000629"
     CDS             complement(1472..1963)
                     /locus_tag="PGA33_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDG6472464.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQSTITWPEQLGHKIAIPHKG
                     ALK"
     gene            2060..3007
                     /gene="corA"
                     /locus_tag="PGA33_000630"
     CDS             2060..3007
                     /gene="corA"
                     /locus_tag="PGA33_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDG6472465.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            3038..4087
                     /locus_tag="PGA33_000631"
     CDS             3038..4087
                     /locus_tag="PGA33_000631"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDG6472466.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRNLNIHRG"
     gene            complement(4135..5343)
                     /gene="gltS"
                     /locus_tag="PGA33_000632"
     CDS             complement(4135..5343)
                     /gene="gltS"
                     /locus_tag="PGA33_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDG6472467.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(5680..6288)
                     /locus_tag="PGA33_000633"
     CDS             complement(5680..6288)
                     /locus_tag="PGA33_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDG6472468.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            6687..7334
                     /locus_tag="PGA33_000634"
     CDS             6687..7334
                     /locus_tag="PGA33_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDG6472469.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSTIKNQPVTSETPEKACTSETPKTPEETDNQL"
     gene            7565..8464
                     /locus_tag="PGA33_000635"
     CDS             7565..8464
                     /locus_tag="PGA33_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6472470.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLKLESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            8457..9689
                     /locus_tag="PGA33_000636"
     CDS             8457..9689
                     /locus_tag="PGA33_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6472471.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGITAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGQLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            9673..9858
                     /locus_tag="PGA33_000637"
     CDS             9673..9858
                     /locus_tag="PGA33_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472472.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            9907..10233
                     /locus_tag="PGA33_000638"
     CDS             9907..10233
                     /locus_tag="PGA33_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDG6472473.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            10263..11282
                     /locus_tag="PGA33_000639"
     CDS             10263..11282
                     /locus_tag="PGA33_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDG6472474.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(11518..12171)
                     /locus_tag="PGA33_000640"
     CDS             complement(11518..12171)
                     /locus_tag="PGA33_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDG6472475.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            12345..13031
                     /locus_tag="PGA33_000641"
     CDS             12345..13031
                     /locus_tag="PGA33_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HDG6472476.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(13097..14356)
                     /gene="sdpC"
                     /locus_tag="PGA33_000642"
     CDS             complement(13097..14356)
                     /gene="sdpC"
                     /locus_tag="PGA33_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HDG6472477.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            14646..15581
                     /locus_tag="PGA33_000643"
     CDS             14646..15581
                     /locus_tag="PGA33_000643"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HDG6472478.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            complement(15837..16256)
                     /locus_tag="PGA33_000644"
     CDS             complement(15837..16256)
                     /locus_tag="PGA33_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HDG6472479.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(16378..17262)
                     /locus_tag="PGA33_000645"
     CDS             complement(16378..17262)
                     /locus_tag="PGA33_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDG6472480.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            17422..19083
                     /gene="hutU"
                     /locus_tag="PGA33_000646"
     CDS             17422..19083
                     /gene="hutU"
                     /locus_tag="PGA33_000646"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HDG6472481.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            19083..20321
                     /locus_tag="PGA33_000647"
     CDS             19083..20321
                     /locus_tag="PGA33_000647"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HDG6472482.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTVSFKA"
     gene            20598..21719
                     /locus_tag="PGA33_000648"
     CDS             20598..21719
                     /locus_tag="PGA33_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDG6472483.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQSAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            21830..22711
                     /locus_tag="PGA33_000649"
     CDS             21830..22711
                     /locus_tag="PGA33_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDG6472484.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKILVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            23198..>23496
                     /locus_tag="PGA33_000650"
     CDS             23198..>23496
                     /locus_tag="PGA33_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472485.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAIV
                     SNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFA"
BASE COUNT         8074 a         3365 c         4453 g         7604 t
ORIGIN      
        1 taaaatatat aaaggtaggt taagacattg attagaaacc gtgttatgaa tagcgttgtc
       61 aataaatatt tgcttcacaa tcgctcgatt atgtttaaaa atgatcaaga tgttgaaaga
      121 tttttttata aaagagaaat tgaaaataga aaaaaacata agcagccttc aacattaaat
      181 gttaaagcaa atttagaaaa attgtcatta gatgatatgc aagtctttcg ctttaatttc
      241 agacatcaaa ttgataagaa aattttatat attcacggtg gattcaatgc actacaacca
      301 tcaccgttcc attggagatt gttggataaa atcactttaa gtacattata tgaggttgta
      361 ctgcctatct atccaaagac accagagttt catattgacg atactttcca agcgatacaa
      421 cgtgtttatg atcaattagt atctgaagta ggacatcaaa atgtcgtagt catgggtgat
      481 ggttcaggtg gtgcactggc attatccttt gtacaatctc ttttagataa tcaacagcca
      541 ttaccgaata aattgtattt aatctcacca attttagatg caacactatc taataaagat
      601 atttcggacg ccttaatcga acaagatgcg gttctaagtc agtttggtgt caatgagatt
      661 atgaaaaaat gggcgaatgg cctaccatta acagataagc gcatatcgcc aattaatggc
      721 acaatagaag gattgccacc agtttatatg tttggtggtg gacgtgaaat gacacatcct
      781 gatatgaagc tatttgaaca aatgatgttg caacatcatc aatacattga gttttatgat
      841 tatcctaaga tggtacatga ttttccaatt tatccaatta gacaatcaca taaagctatt
      901 aaacaaattg ccaaatcgat agatgaggat gtaacacaaa ataactaata acatataaat
      961 gacccagtta cgatttcatg caagatgata tttgacatga aaacgtaatt gggttttgtt
     1021 tttgaaaata tagcaagggt tatatagact aattgttaat gagcttctga gtgaaaatca
     1081 ccaataagtg tattgatatg tgtgatgata aagtattgcc ctttaatttg ataaaattga
     1141 tctaaaaaat tatcgaattg acgtggaata tcactaagtt ctgcaaagtt agctgtttta
     1201 tcataattgc gatgagcaac ctcattttga aatttatcat aatgttctag gaaatgttga
     1261 tcttcaagct gttgttccac aatagtcata atattatcga tggcttgctt ttgtccttga
     1321 acataagcca tatgcacgcc acgcttaagt agctgatttg ctggtccgtt aatgtataac
     1381 cctttttgtg catttaattc tgttaattca aacttaacat catctaataa atctagtaat
     1441 tcgatatgaa tcttttgttg taatgcatct gtcattttaa tgctcctttg tgtggaattg
     1501 ctattttatg tcctaattgt tcaggccaag tgatagttga ttgattttta taatagtgtg
     1561 ctatttgtgt tgaaaatgat tcaggaaaag catcagaacg acgtgtgtgc tgattaattc
     1621 ccatcatcat tacttcattt gttgatgcta gtgtaccatc ttctttagtt aatgttaaaa
     1681 ataaatgcaa ccgcttataa tcgtaatcat aaatatataa agtaacagta aatacatcgc
     1741 caagagacaa ttctgagagg tatgtcgtat gttcttctag tgtaaatagc gtatatgcta
     1801 aattttcgcg ttcttttaaa gaaagaccgt ggctgtaatt aaaacgattc acgacgtcac
     1861 taaaaatgat attataattt gcatcatgca tatgattgtt atggtcaatg aattcagatg
     1921 ttacggtttg agtatgtgta aaaagttgtt tgataataac catctcctta gtgactatca
     1981 tactaaaata aattaagaca tacacgttat ttaattcatg ataaaataat agagaattcg
     2041 ttatttaggg ggagtatcga tggctttaaa gatacggtat caaactacat atgaaccgtt
     2101 taaagtagtt gatgatatca aagagatacc aaaagatgcg actattgttt ggtatgattt
     2161 tgatgaaccg aatgaacaag aaaatgaatg gtttaaagca cattttaatt ttaatgattt
     2221 agaagtggat gatgcaatta acggtatgcc acgagctaaa tataaatctt ataaagatta
     2281 tcaatactta gtattccata gtattatggg gtcaaactat tcaccaatag cactaaatat
     2341 ttttatacaa gacaatgtat tagtaacata tcatcatcaa acgttggaat cattaaataa
     2401 agtagtctat aagtatatga atacattaga tgcagaatta gattgtgcag atgtcgtcat
     2461 attaatttta gatatgatgg ttgataaata tttcaacttt gtatatgcac ttgaagatag
     2521 tgtttatcat tttgaagacc gccatgtaga tgatcgcttt aataaaatgg tcatggatag
     2581 tgtctttaag ttacgttcgg atttgattaa agtaaaacgt gtactgtttc ctatgcaaga
     2641 gctaattgat acaatgaaac aaaatggtga tttgattata gataataaac attcgctata
     2701 cattcaacat atcgatgacc acttgattaa gcaacgtaat atcattcgta ctgctcaaga
     2761 aatgacaaac gaaatacgag aaaattttga gtcatacacg tcatttagaa tgaatagtat
     2821 tatgcaagta ctgacattag tatcagttat attttcaccg ttaacattca tagctgggat
     2881 ttatggtatg aactttgtaa atatgccggc attacattta cattatggat attatatttg
     2941 cttagctgta atgtttgtta tagctgtcgt attaatcatc tttttcagac gtaaaaaatg
     3001 gttttaaatt agaaatgacg aaaaggtagg tttaatcatg agtgattttc aaagagaaca
     3061 gagaaaaaat gaacatgttg aaatagcaat ggcccaatct gacgcaatgc attcagattt
     3121 tgataagatg cgttttgtgc atcattctat cccatcaatt aatgtaaatg atatcgattt
     3181 gacatcacag acgcctgact taacgatggc atatccggtt tatattaatg caatgacggg
     3241 tggtagcgag tggacgaaaa acatcaatga aaagctagct gtagttgcaa gagaaactgg
     3301 cttagcgatg gcagttggat caacacatgc ggcattgaga aatccacgca tggctgagac
     3361 gtttacgatt gcgcgaaaaa tgaatcctga aggcatgatt tttagcaatg ttggtgcgga
     3421 cgtaccagta gaaaaggctt tggaagcagt tgaattactt gaggcacaag cgttacaaat
     3481 ccatgttaat tctcctcaag aattagttat gcctgaaggg aatcgtgaat ttgtgacttg
     3541 gttagataat atagcgtcga ttgtatcacg agtgtctgtt ccagtcatta taaaagaagt
     3601 tggatttggt atgagcaaag aattaatgca tgacttacaa caaataggcg tcaagtatgt
     3661 cgatgttagt ggcaaaggtg gtactaactt tgtagatatt gaaaatgaac gtcgtgcaaa
     3721 taaagatatg gattacttat catcatgggg acagtctaca gttgagtcat tacttgaaac
     3781 aacggcttat caaagcgaaa tttcagtttt cgcgagtggt ggtttacgta caccactcga
     3841 tgcaattaaa agtttagcac ttggtgcaaa ggcaactggt atgtcacgtc cgtttttaaa
     3901 tcaagttgaa aataatggca ttgcacatac agtagcttat gtagaatcat ttattgaaca
     3961 catgaaatca ataatgacga tgttagatgc taaaaatatt gacgacttaa cacaaaaaca
     4021 aatcgtattt agtccggaaa tattgtcatg gatagaacaa cgtaacttga atatacatcg
     4081 aggataaata aaagaggagg cgaaggcctc ctcttttatt tatgagtttg gtgtttaact
     4141 aaaccattgt atgaatccca taatgactat aacaccaatt aaatcgatta agaatgcacc
     4201 aacaataggt acaactaaat atgctttagg tgagtttcca tattttttag taataacatc
     4261 taaatttgcc atggcatttg gcgttgcacc aagcccatga ccgataaaac cacctaccat
     4321 tactgcagca tcataatctt ttcctaaacc tctaaataaa attaaaacag caaataaaat
     4381 cataacgaca acttgaacta aaacgataat aataagaggt atagcaagtt tataaatttc
     4441 gattaattga atgctcatta gcgcaagaga taagaaaata cctaatgcga catcgccaat
     4501 ttgattagta atttttaaat ctacaatatt aaaattaaaa ctttcagaga tatttcggac
     4561 aataacagct acaaataatg agccaacgta aattggaaca ttaatccctg tttgagctgt
     4621 aaacaaatga ctgaaataac ttccaactgc catacagaat acaacgattg taaattgaat
     4681 gaagaatact tcagtggcat taaatttact atgtaaatgt tcgttatatg ctacttggct
     4741 ataatcttta aatgtatcgt cactatgttg aggctttaag ttataacgtt tgattaaaaa
     4801 ttttacaact ggaccaccga taagccctcc aaatacaaga cctaaagttg cagctgcaag
     4861 agccgctgtc agtgccgaat caatacctaa atcttgaatt gtcttaccat aagcagcagc
     4921 attaccatga ccgccttcca tagacatgga acctgctgtt aatcctaaca aaggtttaat
     4981 atttaatact tttgctagtg atacaccaac tatgttttga atgactgaaa tgatagcaca
     5041 aaacataaag tataatagca agactttgcc acctaattta aataatttca atgatgcacc
     5101 aagaccgatt gtcgtaaaga atgctaacat gaagaaatct tgaatgaatg aagcatctaa
     5161 tttaatctta accatgccaa atgaatccaa agccgcaact aaaatagcaa agattaagcc
     5221 gccaatcact ggtgctggta tacaaatacg ttttaaaaaa ttaacgtgat taacgatagc
     5281 cttaccaagt aaataaagga tacaagctaa acataatgtt gtaattgcat taagttctat
     5341 cattttcaaa tcccccttgt tgaatatccc tctccctaaa taagctttta tatgtaagcg
     5401 ttataaaagg gcgcttttaa cattatacat gcaacaaatc aatatttcta gtttttgaaa
     5461 ataatatcaa taatattaat gcatctaaat ttaataatta taaataattc tattgttata
     5521 ttttataatt aagaacaagt tcaataagag gagatgaatc aaaaaatatg atggatacaa
     5581 agaaaaacaa tcaacccatc cgcatgttga cggcacgatt gattgactta taaggagata
     5641 gatttcattt tcatgatagt aatcaatgaa agatggcatt tatttccaag tatcttcggg
     5701 aaacatacaa tctgatttac gcatgcgaga cacaaatgga ttacctttca ctgtgtaacg
     5761 taaagattta tgtgtccaat cacctttatt tggaataccg attcgtggac tagcaataat
     5821 atctttagga tatttacgat tcttagtatc aatagacaat ctacagtcat ttaacgtagc
     5881 gccatcgata gcccgtggaa tgttaaatgc cttagtccat tttcctgggc cattcgttac
     5941 ctcgtagcct ttcttacctc tgttacggaa catagcggat aaaccttctt ctggttcaat
     6001 tgcgcggata agtacgcctt caggtatacc ttcagatttt gttacaaaat taatgagtaa
     6061 atgcgtatgc atgacatgtg cataaattgt accaccacgt ttatataatg acgtgacttt
     6121 aggtgttatt ttaccgccat agccatgagc cgcacgatca ttcaaaccta agtaagcttc
     6181 cgtttccacg atgtagcctg tatacgtttg agtggtatcc tgataaatca ctttgacacc
     6241 taataagttt ttagcaattt gtcttgtatc attattaacg aaatccataa tattctcctc
     6301 gcaattatat gattaattta aataaaaatg aaaaagatat ttttattgtg gtaaatgttt
     6361 gataaaatag tattgttaag aattataaaa atgtaaagta tatactttgt tatggaatat
     6421 tttatttgca tagattgaga ctcttttaaa cgtgaacatt gcagtatgac atatttttag
     6481 tatagttaac gaagcgttga ctttcaaatg agtctccata tagatgtgta cttgtagcgg
     6541 gttttcattt tactagtgat gaaatataaa gttcattttc caaagtgatt gacgattgaa
     6601 atgaagtggc ttgataatga atcttgtaaa ttataacggt aacaataatt aaaaatataa
     6661 atatgattaa cgaaagggag attacaatgg aacaattacc tcaagtaggt tttatgaaat
     6721 cattcgtttt attttggaaa aattatgtaa attttaaggg gagatcaaga cgtagtgaat
     6781 attggtatat ggcactatgg catttgatac ttatggttcc agctgtgtgt gtgctaataa
     6841 taaacctatt tctattcttt atttctatag cggcggataa cgaaacatta atagttatga
     6901 gtatattgat gattatcctt acatctgtgt atacttttgt ctatagtttg gcaacgttta
     6961 ttccaaattt agcgttgtgc gttcgaagat ttcacgatat atcaagaaca atgttatttc
     7021 caatgattat gacagtattc agtattatat tttatatagt attacagatt atcgatagtt
     7081 attatgacag tgattttaca ttaatgccga tgggtcttaa tattttgtta gtacttcttt
     7141 attttgttta tttaggccta acaattgtta tgatagtatt cctatgcttt gacagtaagc
     7201 cggcaaataa atatggcgaa agcccaaaat acccgtctac aattaaaaat caaccagtga
     7261 catctgaaac accggaaaaa gcgtgtacat ctgaaacacc gaaaacaccg gaagaaacag
     7321 ataatcaatt gtaatgatat aaatatctcg gcacaatgat gctgggatat ttttttacaa
     7381 ataaggcaat aaaggcatgg aaaaaacata tctagtgcta atgatactta acatgcaaaa
     7441 atggcactat ttaaattttt caatttcaga aattttaaaa ataaattcat tttaaaaata
     7501 ttgcatttat agagaatgga aatgttatat taagataaat acatattttt ttaggaggca
     7561 aagtatggga cttgtaattg aacatgttac gaaacgtttt ggcaagatga cggcagtaaa
     7621 tgatatttca ttaaagttag aatcaggtaa aatgttaggt tttctaggaa gaaatggtgc
     7681 aggtaaaaca acgacatttc gtatgatttt aggcttaagt gaaccaactg aaggacacat
     7741 tacgtataac ggtaaaaaac tagataaaac aatgtacaat cgtattggtt atttaccgga
     7801 agaacgcggt ttacatggga aattgacagt tgaagaagaa ttgaaatatt tggcaacttt
     7861 aaagggaatg tcaaaaacag aaatacaaca gcaaatatcg tattggcttg agcgttttga
     7921 tattactgaa aaccgcaaaa aacgaattga tagtctatca aaagggaatc aacaaaaaat
     7981 tcagctgtta gcaagtatgt tacataaacc agaattacta atattagatg agcctttcag
     8041 tggtctagac ccagttaatg ttgagttatt aaaagaagca gttaaagact taaatgattg
     8101 gggcagtaca atcgtttata gttctcaccg tatggagcat gttgaagaat tatgtgatga
     8161 tgtttgtatt ttagataaag gtcaacttgt tgtttctggt gatatcaatc atgtacgtgc
     8221 atctaatggt aacaagaaag ttgtcattga atcagaaaca acactaccag atttaacaaa
     8281 cattagaggc atcattcata gtgaaaacat gaagcaaggt cttcagttga cgattgagaa
     8341 tgaagacgta gccaaagata tttatcaagt tgtggcacat caaggttatg ttaaacgatt
     8401 ccaagttgta gaaccatcat tacaagatat attcattgag aaggttggtg gcaaggatgc
     8461 ataagttttg ggcgacattt actttgacat atatcaaaaa aattaaagct aaatcatttg
     8521 taatttttat gattattatt gctgccttaa tgattggcct atctaacatt gataagatta
     8581 ttaacatgtt tgatgatggt ccagataaaa ttggtgtagc agcaccgaat gaacaaatct
     8641 ataaggtttt caagcaacag gcgaatacat ttcatagtga tgctaagttt actaaagttt
     8701 ctatagaaga tgctgaaaaa gaagtaaaaa aacataaact tgataaagct tatattatca
     8761 aggtgaatca aaatcgcacg ttacaaggga caatcattag tgagaagcga gtaagtcatg
     8821 aagattcaca aaaagtacaa gcactgctta cagctattca aacaaatatg gtagccgggg
     8881 aattaaatat caacaaagaa gacttacaaa aattacaagc acaaagtaaa gttgataata
     8941 aggtgatttc taatgatgaa gtcgataaag tatcagaagg acaaaagatt ttcaactatg
     9001 ctttagctta tggcattatc tttctaatgt tcttcattgt tttaaattat gcatctcaaa
     9061 tagctatgga aattgctagt gagaaaacat cgcgtgtcat tgaaatgatt attacaagta
     9121 tttcaccaat ccaacatatt tttgctaaaa ttttaggcgt tatagcagtt gccgtgactc
     9181 agattatttt aattgtattg atggcagtta tatgcatttt tgcctttgac ttaaaagact
     9241 tgctacaagg ttttaacgtg gaaatgaatc aattgtcttg gcaaattatt attgtcggaa
     9301 ttataagtag tattgttgga attacggcgt atgtattact tgcagccatc ctcggatcat
     9361 taacatctag aatagaagat ttaaatcaat ctttgatgcc actgacatta cttggtatga
     9421 ttgcatttta tatcgcgata ttcacgataa ataatcctga tggacaactt gctttaatca
     9481 caagttatat tccgttttta gcgccatttc aattagttgt acgcgctcaa acttcagggc
     9541 ttcaaatata tgaggtcatc ttaagttctt taatctcaat tgtaatggta gctgtcttga
     9601 tatggattgc aattaaaaca tataaagatt cagtacttac atttgaacgt ggcttattta
     9661 attcaatgaa acgtgtattt aaaaaataaa attgatgaga gtgacacaca tttatcatat
     9721 aaaatgatga tgaaagcgtg ttactcttat ttttttatat ttagctatag aataatgtct
     9781 aaaaaagtgt acaatatgaa tgtgaaaacg aactttttaa aatgggaatg tgaagtaaga
     9841 agaaatgtaa acaaatgata tgtagccgat agattattga tagcaaatgt gtgatggagt
     9901 gatttcatgt tatatccgat ttttatattt atattagctg gcttatgtga aataggtggg
     9961 ggatacctga tttggctgtg gcttagggaa ggacagtctt cacttgttgg gctaataggc
    10021 ggtgcgatac tcatgttata tggtgtcatt gcgacatttc aatcatttcc atcattcgga
    10081 agagtatatg cagcatatgg tggcgtattt atcatcatga gtttgatttt tgcgatggtc
    10141 gtagataaac aaatgccaga taaatatgat gttatcggtg caatcatttg tattgtaggt
    10201 gttttagtaa tgttgctacc cagcagagct tgaacacttg tggttagaag ggagaagtaa
    10261 aaatgatagt tgaagttgag caccaaagac aaggtgtaga tttaatcaaa attgataatg
    10321 atgaaactaa aattattttt acgaattatg gagcaagagt agtgtcttgg aaatatcatg
    10381 ataataatat tgttcttgga aatgttgttg aagcggatga attttatcat agtaatcctt
    10441 ttaaatttgg tgcttctata ggaagatata gcggtagaat tgataatgct aaatttaagt
    10501 tgaaaggtaa agaatatcag ttagaaaaaa acaacggtga acatcattta catggtggat
    10561 gtcatggttt ggacaataag ttatttgatt atgaaattag aaatgaaatt gctcaaatta
    10621 aagttatatt taagacggtg ttgaaatcag ctgatgatca tttcccgggt gatatcgatg
    10681 tgacgattac acatatttat gatgcggatc accaatggtc gattgaatac gaagcagttg
    10741 catcagaaga cacattgttt agtcctacga atcatgtgta cttcaatttg aatcgagata
    10801 ataatgtcgt tgataatcat cgcatttcaa gtaatcaatt agatatgtat gtattagatg
    10861 agcgaaatat tgttacaggt gatattctag atttacatga agtgtttgaa gataacaaga
    10921 taaaattgag tgatattttt acatcacaac atgcgcaatt aagccaacaa atgacacgtt
    10981 ttggtggtct cgatcaccca tttacagttg gtgaacataa aatgtatgtt gaaaatcatg
    11041 aatttatgtt agaggtcgat acagatatgc ctcatgttgt gttctttact tttaaccagc
    11101 ctgatgagtg ggatagtcca tttaatattt ataaaccaca ttctggcttt acattagaaa
    11161 cacaattttt accaaatgat attaatttat atggtgaaaa agcaccgtca attttaaaag
    11221 ctggtgaagt gttcaattcg aaaacaagtt tccgtatcga agaaaaagta gaaaatgatt
    11281 aaaagtatcg gcatttatat aatatttatg ttatttggcg gaattttgaa taggttatca
    11341 agaatcgtat caaatgaagt gacatcatta atatacagtt tgaaataaag aaaatatata
    11401 acataaaaaa ggaatcaata gcatcagtgg ttatagataa gactattata caaacgatgt
    11461 gtaagttgat tcctttttgt tttgaaaagt tcatttaaca cttataatga aaggtaatta
    11521 tttcaatgtt ctgttataca ttttttgaag tttatcgcgt cttgttggtg acagaaatgg
    11581 attgtttagt gcatatcgta acatatcttg attttgtcta tcattataat aaaggtcatt
    11641 aagctgttgt acagataaac gatgttcgat tggtgcttct tgtattaagt aaagctgatc
    11701 atttggagat acatagcctt gttttaatac ccggaaatag aaacctgttt taccagatgt
    11761 agacatgcgc ttcactaaat caggaatatt atattttgct tgaattttcc agcatggttc
    11821 tctaatttca gaaacctcaa ttaacgcttc acctaaacga tattgattac caaagtaaac
    11881 atcagattcg tctaaataat caaatgttaa attctctcca aacatcgcat gagtaggtag
    11941 tgtaggtaaa tcatcttgat acagtgcata attatgctta ctaaacccac aaactgcttt
    12001 atgtggtcca ccatgatctt tataagcctg ttcatcacca gtgaaaccag ttgaagataa
    12061 ccatgttgtt tgtgaaatct tagttttatc taaagcagag cgcatcggtc ttttcgagct
    12121 ataaggcaaa tcttggattt ttcctgtcga aattgcgtga attgatatca tatcattctc
    12181 cttaacgagt attatttaaa atatattgta ctataaaaca tttttagaat tgtaaattct
    12241 gattgatatg tttaaaaaaa tgttagaaat aagggtatgt taatttatac gagtcatgta
    12301 aaatatgaat tgaatagatt acataaatat gggaggctac cttcatgaaa gatgtcaaag
    12361 cactcaagtt aatgacatta aatgatgttt taagtcaaat caatggtgac atgacgctgg
    12421 gaattggtac aggaagtaca atggaattac tcttacctca aatggcgcaa ctaattaaag
    12481 aacgtggtta caatattaca ggtgtatgca cttctaataa aattgcattt ttagcaaaag
    12541 aattaggtat taaaatatgt gaaatcaatg atgttgatca tatcgactta gcaattgatg
    12601 gtgctgatga agtagatcca tctttaaata taattaaagg tggcggtggt gcgctgttca
    12661 gagagaaagt tatagatgaa atggcatcgc gttttgttgt ggttgtcgat gaaacgaaaa
    12721 tagttcaata cttaggtgag acgtttaagt taccagtaga ggtggataaa tttaactggt
    12781 atcacatatt acgtaaaatt gaatcatacg ctgatataaa agtagaacgt cgtgtaaacg
    12841 aagatgtcgc atttataact gataatggca attatatttt agattgtaag ttaccaaaag
    12901 gaattgaccc atataagttc catgagtatt taattcatct gacaggtgtg tttgagacag
    12961 gatacttctt agatatggca gatcaagtta ttgtaggcac tcaagagggt gttaaaattt
    13021 tagaaaaata atatatagta tagaaaagcg cttgctagca tcatcttttt aagaggtact
    13081 agcaagcgct ttgttattat ttgtttttat ctgaagattg ttcttcagtt ttttcgatat
    13141 gtttttctgc atcaattaca actgatgaac gtctgtcttc tttataagtc gcaggttctt
    13201 ttaatgattg ctctatttca tcttcatttg aatcgtttgt taaatcgtca ctttgtggtg
    13261 cttgtttaac tgattctgct gattctactg actccgaagt gatgtcatga tcttcatcat
    13321 gattagattc agtttgatta cctatgtgat gttgaggttc tgttttttca gtaccttcat
    13381 gaatgcttgg ttcgtcagcc actgtatttt cagtatcatt tttagcaaca ccaaccgttg
    13441 atgcagtagc tacacctgtt ttttcagcat ctttaatatt tgaagaagat ttttcagcct
    13501 cagtatgatt agtttcttca tcgtcattga catggtctaa ataattatta ggctcaagtt
    13561 cttcaaggtt tcgtcttgtt gttaaatatg caatacctcg gataattaag cttaaaccaa
    13621 tgtatcctac tgcaacgatt gctgttgaaa tggcgatgac tttgattgaa aatagatcgc
    13681 cgatttcttc gctaaacaag aaaataagtc cgaatgtcat tgaagcatgg aatgtcgttg
    13741 caatataaat tgtacgtcct ttagtcgctc taattaattc accaagaatc atagagaatg
    13801 agaatgtata aaggaagtta taagcagcaa attctgtacc ataagttgta tttgctgaga
    13861 aaactgaata catcaaacca acaacaatac ttgcaaagaa cgtgttcatt ttcgtttcaa
    13921 caatattttg taagtatgaa cggaatccga attctactac gaacgccatc agaatatgtc
    13981 caatcagaat gtgtgtaata ggtactgata agcctgttga ttgtaataaa ataaagctat
    14041 ctgcaaatgt attaaagctg tacataccaa taattagaat aattaaaggt aaaattaaag
    14101 ctagcaatag acgttcaatt actttcaggc tgattgagaa ttttaaacct gcaagttgga
    14161 cctttttata tttgaaaacg agtatacaaa tgattgcagc aattaatggt gctagatctg
    14221 taacttcaaa tataaagtgt ttgacaccaa ttatagactg gaaatctctg agcataatgg
    14281 ataacgccat tgtaatgaca aagaagacga aaatcgtcat tgcccattga aaaccagaaa
    14341 ttttattgtt cttcattata tgtaacctcc attaggtaac aagcaaaata tcttttagta
    14401 acaattatac atattacaag ccgagagata aattgtctat gtcaatatta atgaaaatgt
    14461 aatagtcttg acgtatttta ttaacataaa tcagctaagt attcgttatt acatagaaaa
    14521 taggggtaaa agtaatgtag ataagtattt ttaagatacg gatttaacta tgaattgaat
    14581 tcaaaatttc ctatacgtta tactttaatt gttaataagc cacataaata agagggggaa
    14641 atgctgtgta caagcaaggt gaaccaaatt tatggactgg aaggttagat agtgaaacag
    14701 acccgaaaaa atttagacat tttcaaacag taacatttga agatttgtct aagctggaaa
    14761 agagtagtat gccatcaggg gtcggtatat taggctatgc tgttgacaaa ggtgttgctt
    14821 taaacaaggg gcgcattggt gcaaaagaag gaccagatgc gattaaacaa gcatttgcag
    14881 gtttgccgga tttgaatcaa tgtgaaactt tagtcgatta cggaaatgtt tatcatgatc
    14941 atgaggaatt aattgatact caaaaagaat ttgctatgct tgcagcgaag tcaattgcta
    15001 atcatagaca aacattttta ttaggtggtg gacatgatat tgcgtatgct caatatttag
    15061 caacacgtaa agtctatcca acacaatcta ttggtgtgat aaatattgat gcgcattttg
    15121 atacacgtgc tgaacaacaa tctacatctg gaacgagctt tagacaaatt ttagaagaag
    15181 atgaaaacac agattattta gtgcttggta ttgctcaagg tggtaatacg caaagtttat
    15241 ttgattatgc taaagagaaa aagattgatt atgtctttgc agatgaatta ttgagtcacg
    15301 tatcaccaac aattaaagat atgattgaac gttttgtaca tgaacatgat gtcattatgt
    15361 ttacgatttg tatggatgtc attgatagtg cgtttgcgcc tggggtaagt gcgccggcag
    15421 tgttaggttt gtatccacat actgttcttg aattagcaaa acgtattatt ccaagtgata
    15481 aggtgtcttc agttagtatt gctgagatga atccaacata tgacgctgac aatagaactg
    15541 ctaagctcgt tgctaattta gtgcatcatt ttttaaaata attaaaaata tttatatgtg
    15601 acgagatttg cattgcaggg cagaatgtga gtatttctta atagaagtta tattaacgtt
    15661 gatatatttg aaaaataatt gcagtaagaa gactcttaag ttgtagtcat cttactgctt
    15721 atttatgttt gttgaaatag caaaaataac aactgtattg tttgaaaagt tctcttatta
    15781 tataataatt aattaaaatt ttaacggagt tcaagaggcc tttttataat gacaccttat
    15841 ttgtaaaatg tcatatgtgg tttagcctct ttataataat ttaatctgtt ctcaagtgtg
    15901 ccagtatgta attctagctt atgaccatca gggtcggtaa agtaaattga ttgtctatct
    15961 ctaatatctc taactcttcc ttctaaaata ttcacgttat tatcttttaa cctctgatgc
    16021 caatatttaa attcgctgtc atctatagtg aaagctatat gtgtatatga aaagtgaatt
    16081 tcattacgtg gtatatcttt ttcttcattt aaagcaatcc ataggcctgc aagctcaaaa
    16141 taagcagttt ttttaccagt caatagcaat ttcccaagta aaatatctct ataaaaatgt
    16201 attgaatcgt ttaaatttct gactgaaaag catatatgat taatagattt taacatctgc
    16261 ttcacttctt tcttagaatt tttataataa attatttttg tatcgatttt tgaaatgacg
    16321 tcattaattt ttatacgatg atctgcgcaa ctgcataaga ggctctaata acttaagtta
    16381 aagagcctct aaaagtccat ataaataatc ttggcattgt tgtacgaatg tatgtacttg
    16441 ttcggagtgt ttgcgttttt tatggtaatt gatataaatt tttcggccta agtttggacg
    16501 aattttttta tattctaaat tagacgtgtg aaatgattgg taataaaatc tcggaatgat
    16561 agcgtaacct aagccgagat gaacaaatcc aactgctgat tcgaatcgat ctgtttctac
    16621 aacgacattt ggacgaatat ttcttctatt aaaatagtca tctaagtgtt tgcgcacttg
    16681 agaatttttg tttggtaata tgagtggcaa attttcaaca tctacccaat tttgattttt
    16741 aaatgtttcc ttgggtgcta ataaaatgta agattcctca tataaaggaa tggatcttat
    16801 atcttcgtgg gttatttttt catttgttat agctaaatga atattaaaat tcagtaattg
    16861 ctctatagat tgatgtttat catgtatttc atataaacga tattgctgtt cagggtagtc
    16921 ggaatggtgc tttcgaatta aattcgcaat ccattgattc gtagattcaa gagtcccaat
    16981 ttttatcctt ggttctgatg taacgcttaa atcatacatt ttttccatcg tggatcgata
    17041 ttgttgaact aattcgctcg cataacgata aaactgtatt cctttttcgg taatcttgat
    17101 gtcttttgtt gaacgtgtaa ataagtcata acctaaatct gcttccattt ttttaatcgt
    17161 agcagttaaa gatggctggc taatatgtaa aaattgtgca gctttagtaa aactattata
    17221 tttcacgata gccaagaagt attctaactg aataattttc atgtgttttg ctccttttat
    17281 ttatagattc aagctatgga taattagttt atttatattt gttggctata ttttaacata
    17341 ctataatgaa tattgaagat aataatgtaa gcgttttaaa tttagtggaa ttaacttctt
    17401 aaaagaacag gagcgaattt tatgagaaaa attcaagcaa aaaaaggttt gagtattgag
    17461 tgtaaaggtt gggaacaaga agcagtactt agaatgttat acaacaattt agacccagaa
    17521 gttgctgaaa gaccagaaga tttagttgtt tatggtggta ttggaaaagc agcacgtaat
    17581 tgggaagctt ttgaagcaat agagaaaacg ttacgagaat tagaatcaga tgagacaatg
    17641 ttagtacaat caggaaagcc agttgctgta tttaaaacac atgaagaagc gccacgtgta
    17701 ttaatttcaa attcagtatt agtgcctgaa tgggcaaact gggatcattt taatgaatta
    17761 gataaaaaag gtttaatcat gtatggacag atgacagccg gtagttggat atatattggt
    17821 tctcaaggta ttgtgcaagg tacttatgag acatttgccg agctaggtaa tcaacacttt
    17881 aatggagatt tagcaggcac tgttacactg acagcaggtt taggtggtat gggtggtgcg
    17941 caaccgttag caattactat gaatcatggg gtagcaattt gcgtggatgt tgatgaaaca
    18001 cgtgttgata agcgaattga tacgaaatac tgtgatgtta aaacagctga tttagatgaa
    18061 gcattaaaat tagcagaaga ggcgaaagaa cgtggagaag gattatcaat cgggttagtt
    18121 ggaaatgctg tagacatcca tcaagcgatt ctagaaaaag gatttaaaat tgacattatt
    18181 actgaccaaa caagtgcaca tgatccgcta aatggatatg tgccacaagg atactctgta
    18241 gaagaagcga aagtattgcg tgaaaaagat ccgaaaaaat atgttgaact gtcacaagct
    18301 tcaatggcaa agcatgttga attaatgctt gaattccaaa aacgtggcgc tgtagcattt
    18361 gattatggta acaatattcg tcaagtagcc ttcaataacg gagtgaaaaa tgcttttgac
    18421 ttcccaggct ttgtaccagc ttacattaga ccattattct gtgaaggtaa agggccattc
    18481 cgctttgctg cgttgagtgg tgatccaaaa gatatcgagc gtgcggatga agaaatgcgc
    18541 aaacttttcc cagaaaatga aaagttatta agatggcttg atttagctga agaaaaaatt
    18601 tcatatcaag gactaccatc acgtattgct tggttaggct atggagaaag agcgaaaatg
    18661 ggcttagctt taaatcgtct tgtacgtgac ggtgaaattt cagcaccaat tgttattgga
    18721 cgagaccatt tagatgctgg ttcagttgct agtcctaacc gtgaaacaga aagtatgaaa
    18781 gatggtagtg acgcggttgg tgattgggct gtattgaatg cactcattaa cacagctgca
    18841 ggtggttcat ggatttcatt ccatcacggt ggcggtgttg gcatgggata ttcacttcat
    18901 gcgggtatgg ttgttgtagc agatggatca gagcgtgctg aaagaagatt ggaacgtgta
    18961 ttgacgactg acccaggtat gggtgttgcc cgacatgttg atgctggcta tgacatcgct
    19021 attcaaacag ctaaagaaaa aggtattcat attccaatga ttgataaagc aggtgataag
    19081 taatgaatga tttaataatt aatcatatag cagaattaat tttaccgaga tcaacagata
    19141 aacctttgaa gggtaaggaa ttagatgaat taaatgttgt gaaaaatggt acagttgtca
    19201 ttaaagatgg caaaattgta tatgctggaa cacatacaga tgactacgat gcgactgaga
    19261 cgattgatgc tagtgggaag gtagtgtccc cagcattagt agatgcacat acccatttaa
    19321 catttggtgg atctcgagaa catgagatgt cattaaaacg tcaaggtaag tcttaccttg
    19381 aaatattaga aatgggtggt ggcatattat ctaccgttaa tgctactaga gaaacatccg
    19441 aagatgactt gtttaaaaaa gcagagcatg atttgcttac tatgattaaa cacggcgtgc
    19501 ttgcagttga aagtaagagt ggttatggat tagatagaga aaatgaactg aagcaattga
    19561 aagtgtctaa tcgtttagct gagaaatatg atttagacat gaaacatact ttcctagggc
    19621 ctcatgctgt acctaaagag gcaagttcaa atgaggcatt tttagaagaa atgattgcgt
    19681 tacttccgga agtaaaacaa tatgcagact ttgcggatat tttctgtgaa acaggtgtat
    19741 ttacaataga acaatcgcaa cattatatgc aaaaagccaa agaagcaggt tttaaagtga
    19801 aaatacatgc ggatgaaatt gatccgttag gcggactgga attagcaatt gatgaacaag
    19861 caatatcagc agaccactta gtagcttcta gtgataaagg aaaagaaaag ttgcgaaata
    19921 gtgatactgt agctgtcctc ttacctgcaa cgacgttcta tttaggtaaa gaagattatg
    19981 cagatgcaag aggcatgctt gacaataacg gtgcgattgc attagcaact gattataacc
    20041 ctggtagtag tgtcacaaac aacttacaac ttgttatggc gattgcagcc ttgaaattaa
    20101 agctatcacc taatgaagtt tggaatgctg ttacggtcaa tgctgctaaa gcaatagaca
    20161 ttaacgcggg tacaattaac acaggtgata aggctaattt agtaatttgg gatgcaccaa
    20221 atcatgaata tattccatat cattttggta tcaatcacgc agaaaaagtt attaaagacg
    20281 gtaaggtcat cgtagataac acagtatctt ttaaagcata aaataattag aaagctctaa
    20341 gcattggttt aaactaatgt ttagagcttt ttgtggtttg taaaaacaag tatattcgag
    20401 aacacgtttt tgtatagagt ctcaattata aaagttagaa tagtttgaca acagtgttac
    20461 gatattatta aacaactgtc tatcattaac gaaagcaatt aacaagagta cattgttgtg
    20521 aaatcatcgt ttaatagtca aagatgatca tcgtagggta gacaacatat cattgactta
    20581 aggaagggga atgaccgatg ggattaaaag atcaagctgt tacctggaga aggtattttc
    20641 atcaatttcc agaactatcc gataaagaat ttaagactac acaaaaaatt aaagatattt
    20701 taacagaaca tcatattaga atattagact taccacttgc gactgggctt gttgctgaag
    20761 tgggacaagg tctgagttgt atagctgttc gggctgacat tgatgcttta ccgattcaag
    20821 agcttgttga acaggatttt aaatctgaaa atgaaggtgt tatgcatgcg tgtggtcatg
    20881 atattcacat ggctagtatt ttggctacag ctgtaaaatt aaaagaaatt gagggcactc
    20941 ttactgggcg tgttaaattc atttttcaat cagcagaaga actaggacat ggcgctttta
    21001 aaattattga aacacatgca ttaaaagatg tacaagcagt attgggtttt cataatgacc
    21061 catcgcgttc ggtaggtaca tttgcaatca aaacaggggc aattacatca gcagtagatc
    21121 gttttgagtt tcatattaaa ggcgtgggtg gtcatgctgc aaagccagaa caatgcaacg
    21181 atccagttat tgtgttggcg caattgatta atagtattca atccatagtt agtagaaatc
    21241 tatctgcgtt tgatgaagcg gtagtaacaa ttggacaaat atcatgtggt aatacttgga
    21301 atgtcattgc tgatcatgcg tatgtacaag gcacagtgcg tagttttgat cctgtggtac
    21361 gaaagcttgt agaaacacga ttacaagata ttgcggacgg attggctcaa gcatataata
    21421 tgaaaatcaa tttaaattat acgcatttac ctggcgctgt gatgaatgat gaggcattga
    21481 cgcataaagc aattgcagta gcccaacatg ttggatataa ggtggaaatg atggaacagc
    21541 cgcttacgat tggagaagat ttttcaggtt atagtcaaca ttttccaagt gtatttgcgt
    21601 taattggttc tcatagtgaa tatgatttac atcatcctca atataaaccg gatgaaagga
    21661 ttctggaaaa agtacctgag tattttgttg aatttgtaaa gagactattg catgaataat
    21721 tcgagcttgt ttttataaag aagaaagtaa tttttagtgt aatcatgatt aagttcctag
    21781 gaagattggg taaataataa ccattaagcg aataaggaga tgtaaaaaaa tggcagctca
    21841 agatcctaga acaaaattta aaacaacgga ttatgaaaaa caagaacaag aggtaccggg
    21901 tttacaatct gaaatgacgc cagcacccga ttgtggcgaa acgtcttacc aaggtcatca
    21961 acgcttacaa ggctataaaa tattagtcac aggtggtgac tcagcaatag ggcgtgcagc
    22021 agcaattgcg tatgctaaag aaggtgcaga tgtagcgatt aactatcttc cgagtgaaga
    22081 acaagatgca caagaagtac gccaagtgat tgaagaaagt ggtcaaaagg cagtgttaat
    22141 tcctggtgat ataagagatg aacagttcaa ctatgacctt gttgaacaag catatcaaca
    22201 attaggtggc ttagataatg ttactttagt tgctggacat caacagtatc atgatgatat
    22261 tcatggattt acaactgagg catttacaga aacatttgaa acgaatgtct atccgctatt
    22321 ttggacagtt caaaaagcat tggaatattt aaaaccgggt gcgtcaatta caacgacatc
    22381 atctgtacaa ggttataatc ctagtcctat tcttcatgat tatgccgctt caaaagcagc
    22441 aattatttca ttaactaaaa gtttttcaga agaattagga ccaaaaggaa ttagagtgaa
    22501 ctgtgtagcg cctggtccat tctggtcacc attacaaatt tctggtggac aacctcaaag
    22561 taaaatccca acatttggtc aaaaaacacc tttaggtcgt gcaggtcaac ctgttgagtt
    22621 atgtggaact tatgtgctat tagcgtcaga agaatcaagt tatacaaccg gacaagtgtt
    22681 cggtgttagc ggtggagtgc aaatagatta ggtttgtatg aagcttttaa ttgaacaaca
    22741 taatattaat ttagttactt caatccatta aattaaaatg aattggtgaa gtgtatttta
    22801 aatagtcggt aaaggtgtgt atctttatcg gcttatttta atactctgtc ataaggagcg
    22861 tttgctcagg gtgtggatta taagttttaa gcagaataaa ctttcttaat gaggatgtca
    22921 ggacaacaac gaaaaagtta aaaactgttg cgggattggg tttagggacg gttagtggaa
    22981 gaaagttaaa ttcattaaca agtgaaatgc ctaaggctag atattaaacg tcagaacgga
    23041 caaagaataa taggatatgt tttcaattaa atagcgaagt atttatatgt taagatgatg
    23101 taaattacat agaaacgcta cccgtagaag tactgatcat agaaacgctg ataaacgtaa
    23161 atttgcaaac tcgatattaa acaaaggggt tgttattatg gcattaatag tatctagagc
    23221 tatattattt ttgattttag cattagcttt tggaatagcg actgtttttc ctgaacaaaa
    23281 attaccatta tatatagtat tcacattatt tgtagcagtt gcgatcgttt cgaatgtatg
    23341 gtttacacat caaaacgtta aaaagcatat cattaagttt aacaaatacg tcgcatactt
    23401 tttatccttt gtctcagggt tactttacat tggatttgta atttatacat taactatagg
    23461 gagctatgat gtcttaccaa ttattatatt tgcaat
//
LOCUS       DAMQYT010000020        22261 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMQYT010000020 DAMQYT010000000
VERSION     DAMQYT010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22261
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.020"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            22..726
                     /locus_tag="PGA33_000651"
     CDS             22..726
                     /locus_tag="PGA33_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDG6472486.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYS
                     IAIHSDVTSNKKRYYKSYSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTI
                     VPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALAQKYREIDIKLSTEDKKL
                     LSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISED
                     DMNEIEKQLKIKFFSN"
     gene            1119..1655
                     /locus_tag="PGA33_000652"
     CDS             1119..1655
                     /locus_tag="PGA33_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472487.1"
                     /translation="MHNNNEFNKKLKDFIGSDKRMALVKGYVNEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNTIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
     gene            complement(1768..3309)
                     /gene="guaA"
                     /locus_tag="PGA33_000653"
     CDS             complement(1768..3309)
                     /gene="guaA"
                     /locus_tag="PGA33_000653"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDG6472488.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCRGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(3334..4800)
                     /gene="guaB"
                     /locus_tag="PGA33_000654"
     CDS             complement(3334..4800)
                     /gene="guaB"
                     /locus_tag="PGA33_000654"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDG6472489.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(4838..6106)
                     /locus_tag="PGA33_000655"
     CDS             complement(4838..6106)
                     /locus_tag="PGA33_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDG6472490.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILLLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(6106..6684)
                     /gene="xpt"
                     /locus_tag="PGA33_000656"
     CDS             complement(6106..6684)
                     /gene="xpt"
                     /locus_tag="PGA33_000656"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDG6472491.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(6915..7017)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7197..7604
                     /locus_tag="PGA33_000657"
     CDS             7197..7604
                     /locus_tag="PGA33_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDG6472492.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHQEVHENNSAYEEIDITHFAEASKGPKA"
     gene            7747..8409
                     /locus_tag="PGA33_000658"
     CDS             7747..8409
                     /locus_tag="PGA33_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472493.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            8527..9483
                     /locus_tag="PGA33_000659"
     CDS             8527..9483
                     /locus_tag="PGA33_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472494.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKALAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQSKNDNAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
     gene            complement(10274..10375)
                     /locus_tag="PGA33_000660"
     CDS             complement(10274..10375)
                     /locus_tag="PGA33_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472495.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNSVVIGGKGK"
     gene            10460..11848
                     /locus_tag="PGA33_000661"
     CDS             10460..11848
                     /locus_tag="PGA33_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDG6472496.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLIYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FDSNDNAALTSH"
     gene            complement(11929..12684)
                     /locus_tag="PGA33_000662"
     CDS             complement(11929..12684)
                     /locus_tag="PGA33_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HDG6472497.1"
                     /translation="MSEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            13176..13745
                     /gene="ahpC"
                     /locus_tag="PGA33_000663"
     CDS             13176..13745
                     /gene="ahpC"
                     /locus_tag="PGA33_000663"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDG6472498.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            13761..15284
                     /gene="ahpF"
                     /locus_tag="PGA33_000664"
     CDS             13761..15284
                     /gene="ahpF"
                     /locus_tag="PGA33_000664"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDG6472499.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKDLLTEIT
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKKLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMNTGEEHLLNLDGIFVQIGLLPNTSWLNDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            15414..15656
                     /locus_tag="PGA33_000665"
                     /pseudo
     CDS             15414..15656
                     /locus_tag="PGA33_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15729..16355
                     /locus_tag="PGA33_000666"
     CDS             15729..16355
                     /locus_tag="PGA33_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HDG6472500.1"
                     /translation="MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            16610..16993
                     /locus_tag="PGA33_000667"
     CDS             16610..16993
                     /locus_tag="PGA33_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472501.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFTSTIITLAIGGKSVEKNDSP"
     gene            complement(17059..17640)
                     /locus_tag="PGA33_000668"
     CDS             complement(17059..17640)
                     /locus_tag="PGA33_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDG6472502.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYDKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSRAEAVDLKVENCKPYIIE"
     gene            17818..18027
                     /locus_tag="PGA33_000669"
     CDS             17818..18027
                     /locus_tag="PGA33_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472503.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILAFLFPKFPHINLLLF
                     NPILSTFSNIIIHIDHFHANKKDDN"
     gene            complement(18066..18317)
                     /locus_tag="PGA33_000670"
     CDS             complement(18066..18317)
                     /locus_tag="PGA33_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDG6472504.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIILIAIVTLILRAMRK"
     gene            18617..18880
                     /locus_tag="PGA33_000671"
     CDS             18617..18880
                     /locus_tag="PGA33_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDG6472505.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            complement(19018..19590)
                     /locus_tag="PGA33_000672"
     CDS             complement(19018..19590)
                     /locus_tag="PGA33_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDG6472506.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTMNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(19771..20139)
                     /locus_tag="PGA33_000673"
     CDS             complement(19771..20139)
                     /locus_tag="PGA33_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDG6472507.1"
                     /translation="MKTILKTITYLVLTIIGAYAALFILKTIDSHGITDQFNPLVKKD
                     DSYVKTTEVSTRMDDQLRSYSQSVFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            complement(20508..21119)
                     /locus_tag="PGA33_000674"
     CDS             complement(20508..21119)
                     /locus_tag="PGA33_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDG6472508.1"
                     /translation="MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQ
                     DKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLE
                     GTYTVAGRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV"
     gene            21319..22026
                     /locus_tag="PGA33_000675"
                     /pseudo
     CDS             21319..22026
                     /locus_tag="PGA33_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505297.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            22065..>22261
                     /locus_tag="PGA33_000676"
     CDS             22065..>22261
                     /locus_tag="PGA33_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDG6472509.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNYFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLAT"
BASE COUNT         7535 a         4007 c         3397 g         7322 t
ORIGIN      
        1 tgattaggag aacactattt aatgtctaaa aatatcaatc aagcaaatag taaattaaat
       61 acgtcaaaca aaaaactaaa acaagcttat tcaaaatccg acagtaaaaa catgaaagtt
      121 atatacatgc ctcattggct agagttttat tcaattgcta tacatagcga tgttacaagc
      181 aataaaaaaa gatattataa atcatatagt agaggaacag tggtatatgt aaaactaggg
      241 agtaatattg gaagtgagtt ttctggtaat catttttgtg taatattaga taacaaagac
      301 aataaaggaa aagaaacagt tacaatagtt ccactttctt ccaaaggtaa taaaaattat
      361 ttgaagttaa acgaatcggt gctaaatcta acagcaacag atttaaaaaa acagattatc
      421 gatatcagta gtaaagtaca agcattagcc caaaaatata gagaaattga cattaaactt
      481 agcacagaag ataaaaagct tttgtctaac ttaaatcaaa aagctaatga actgtataga
      541 gtgataggtg tttattccaa gcacaaaggg aaagatacat acgttaatat atcagcaatt
      601 acaacaataa gcaaaagacg tataagcaaa ataaatgatt ctgatcctac tggaacgata
      661 ataatttcag aagatgacat gaatgaaatc gaaaagcaat taaagataaa gttcttttct
      721 aattgaattg taatattaca tctgatataa ttgatttaca aattcccggt aaccattccg
      781 gcttcattct cttgaagaat gacatattct cagcgtttta gctgaaggtc agatgatacg
      841 tcatctggcc tcttttttta tataaatgcc ctttaagtga aatatacaac aatctgcaat
      901 caatattaga catcacaaaa cacacaataa attacacaat gtgttataag ccacatgtca
      961 ctattatcgc agctagctgc gtcactcaaa acgaatacgg aacgcgttgg ttatttcaat
     1021 cataatatta ctctgcaaat acacagccct gtaaccgagt aaatggatag agatttgaac
     1081 aaatgaaaac aatcaactaa tggaaaggat aaaatattat gcacaacaat aatgaattca
     1141 acaaaaaact gaaagatttt ataggtagcg ataaacggat ggctttagta aagggttatg
     1201 ttaacgagta taaattaaca actgttttaa gagctttaaa taattccgat tacacaaaag
     1261 ggacgataca cactcgttca ataggcaacc taaaacaaat agtagataat cgaattattc
     1321 ctaaaaatat aactcaaaac acaatcttca aattgagtaa tttaacctta gaggttaacc
     1381 tctatgaaag aaacacaatt tatcacggag actttgctat ttactatcct gtagaatctg
     1441 ctctaataat agaaacagat actcctaggc taattaatca tataaataac aacccagtcc
     1501 aaaaaatatt tataataaca acaaatgact ggagctttaa tacacttgaa ttagaaaaaa
     1561 tagtagacga aactataatt tatgatttaa aacaagaaga tcctaaacaa tatgaaattt
     1621 tatataaaaa taaacacgga aaattgcctt attgatgaat taaaacgcaa acttagaaca
     1681 ccttacccct tttccgtcct tattgtaatt ttccaactac tcaaattaaa aaccacccgt
     1741 gcaaacaggt ggttttatta tatataatta ttcccactca attgtgcttg gtggttttga
     1801 tgtaatgtca tagactacgc ggttgacgtg atctacttcg tttacgatac gactagaaat
     1861 cttttgtaag acttcccaat cgatgcgtgc gaagtcactt gtcataccgt cgatagatgt
     1921 tactgcacga atacctactg tgtgatcata cgtacggtag tctcccataa cacctactga
     1981 ttgaatgttt ggtaacactg tgaagtattg ccaaatttct ctttcaagac cttcttctct
     2041 aatcacttgg cgtaaaatcg cgtctgattc tctaacgatt tctagtttat cttcagtaat
     2101 ttctccaagt acacgaatac caagaccagg tcctgggaat ggttgtctcc atactaaatg
     2161 ttctggaata cctaactcaa tacctaattt acgtacttca tctttaaaca atgtattgat
     2221 tggttcgatt aattcgaatt ccatgtcttc tggtaatcca ccaacattgt ggtgtgattt
     2281 gattgtttgt gctgttttag tacctgattc gatgacgtct gtatatagtg ttccttgcgc
     2341 aaggaagtct acacctttca gttttgatgc ttcatcatca aatacgtata caaattcatt
     2401 accaatgatt ttacgttttt gttcaggatc tgaaacacct tttaatttat tcataaagcg
     2461 atctttcgca ttaacacgaa taatattcat gttgaaacct tcaccgaatt gctccataac
     2521 catgtcgcct tcacctttac gaagtaagcc atggtctaca aagatacatg ttagttgatc
     2581 acctattgct ttatgcaata gtacagctac aacagatgaa tctacgccgc cactcatcgc
     2641 acataataca cgacggtctc ctacgcgttg acgaatcttt tcaatttcga tttcgataaa
     2701 gttttccatt gtccattgac ctctacaatc acaaacacga cggacaaaat tatttaataa
     2761 atcattacca tattctgtat gacgtacttc tggatggaat tgaacaccat aaatgcgacg
     2821 tttcttatct tcgattgctg catagtctgt gcttgggcta tcagcgataa cttcaaagcc
     2881 ttctggaatt tcaataactt tatcagaatg actcatccaa acagtttgtt ctgctggtaa
     2941 gccagcgaat aactcatctg acttcgcatt aatgattgct ttaccgtatt cacgttcatt
     3001 ggcacgttca actttaccac ctaataattt agtagttaat tgcatgccgt aacaaatacc
     3061 aagtactgga attcctaaat tatatatttc cggatcaatt gtaaatgaac cttcttcata
     3121 aactgaattt ggaccacctg ataagataat accttttgga ttcattttct taatttcttc
     3181 aattgaaatt tcatgatcgt gtaattcact ataaacgccc atttcacgaa ttcggcgtgt
     3241 aattaattgg ttgtattggc taccaaagtc taagacaagg attaactctt gttcttttgc
     3301 catttccata tttgtcgttc tcctttatct taattagaat gagtagttcg gtgattcttt
     3361 cgtaatttga atattatgtg gatggctttc tgctaaacca gcaggaccca tacgtgtaaa
     3421 ttgtgcttct tcgcgtaatt ctcttaaatc gtgtgaacca gtataaccca taccagcacg
     3481 cacaccgccc attaattggt aaattgtatc ttgtaacgca cctttataag ccgtacgtcc
     3541 ttcgatacct tcaggaacaa atttcttagg cgctttgtcc tcttggaagt aacggtcgtt
     3601 tgaacctttt tccatcgcac ctaaagagcc cataccacgg tatactttat attgtctacc
     3661 ttggaaaatt tctgttgcgc ctgggctttc ttcagtacct gctaataagc tacctaacat
     3721 aaccgcatgt ccaccagcag ctaatgcttt aatgatatct cctgagaatt taataccacc
     3781 atcagcaatg atagctttac catgtttgcg tgcttcagtt gcacaatcat aaattgctgt
     3841 aatttgtggt acaccaacac ctgctacaac acgcgtcgta caaattgaac ctgggccaat
     3901 accaacttta acaatatctg cacccgcttc aaataaatct tttgttgctt ctgcagttgc
     3961 tacgttacct gctactaatg tgatttctgg gtaagtcttc ttaatatgtt tcacttgatc
     4021 gataacacct ttagagtgac catgtgctgt atcgataact aagacatcca cacctgcttc
     4081 gactaatttt tgagcacgaa tatcagtatc ttttgaaata ccaattgctg cggctacaag
     4141 tagacgacca tgttcatctt ttgctgcatt agggaattcg ataacttttt caatatcttt
     4201 aatagtaata agaccttcta gacgtccgtc tttaactaat ggtaactttt caatcttatg
     4261 tttttggaga attttttctg cttcttcaag tgttgtattc actggagctg taattaaatt
     4321 ttcttgcgtc attacatcta caattttaat cgagaagtct tcaataaaac gtaagtcacg
     4381 gtttgttaaa atacctacta agttgcgatc ttctttatta tcaacaattg gtacacctga
     4441 aatacggtat ttacccatta atgcttctgc ttcataaacg ctttcttctg gcgttaagaa
     4501 aaatgggttt gaaatgacac cattttctga gcgttttact ttttgaactt cgtccgcttg
     4561 ttcttcaacg cccatatttt tatgaataac acctaaacca ccttgacgag ccatagcaat
     4621 cgccatttta gattcagtta cagtatccat accagcagaa ataactggaa tatttaattt
     4681 aactttgtct gataattgta cgcttaaatc aacgtctttc ggtaaaatat cagattgtgc
     4741 tggaattaat aacacatcat caaacgttaa tgattctttt gcaaatttac tttcccacat
     4801 taaaaacagc ctccattttt caaattaatt agttatatta tttcacattt tcttccgttt
     4861 gtttatactt tattccatta aaaaagaaat taagaataat tgcagagatt gcgcctaaaa
     4921 caattccgtt ttgagtcaac catgcaaatt gttcacctaa acctttaaat gcttgtggta
     4981 cagcgcttat accagtacct aatcctactg atacagcgat aattaataaa ttgttttgat
     5041 ttttaaaatc gatatgtcct aatatactaa caccatatgc cattaccatg ccaaacatag
     5101 ctatcatcgc accgcctaac acaggtagcg gtatgatatt tgctaatgcg ccaagcttag
     5161 gtatacaacc acatataagt aataacacga ccatgccgta tataacattg tttttcttag
     5221 cgccggataa agaaacaagt cctacatttt gcgaataggc tgtatacgga aatgaattga
     5281 atatagaacc taacactatc gctagacctt ccgcagtata acctttacga aaatcttttc
     5341 tttctaactt cttaccggta atttcactta acgcatgata gacacctgtc gactcaatta
     5401 aactaacgat agctacaata aagaacacta acgtcgatgt cacatcaaag ctaaatccag
     5461 agaatctaaa cggcactggg atgcctaacc aaccggcatg attgacttga ttaatatcga
     5521 ccatcccaag taagccagca cctatcgttc ctaaaacgag tccaattaat atggcaatac
     5581 tcttaataaa tccagttgtg aatctttgta aaagaagaat aatgattaat gtcattaaac
     5641 ctaacaaaat gttcttaaca tctccatagt cctttgcacc ttgacctcca gctaagtaat
     5701 tcattgctac tggcattaaa ttgataccaa tgatagtaac aacactaccc gttactactg
     5761 gtgggaagaa ttttacaaga tgtgaaaaga aaggcgcgat gataataact aatatccctg
     5821 ataaaaatag cgaaccataa agtacatcta ttcctttcgt ttgaccaatt aaaatcatgg
     5881 gcgcaacagc cgtgaatgta catccaagaa cgattggtaa tcctgttcct gttactttat
     5941 tggcttgtaa aaatgtggca accccacaca taaatatatc tactgtaact aagtaagcga
     6001 tttgttcagg tgtaaacttc aaacttgtac caacaatgat tggaactaag atagcacctg
     6061 cgtacatagc taaaagatgt tgaacactta ggattaaatt tttcattatt cttctcccac
     6121 caatgtcact ttgtttcctt ctagtgaagc aaccttgcag agagaagaaa ctgttaaacc
     6181 tgcttcttct aaacgttgat gcccattttg gaaactcttt tcaacaacaa taccaatacc
     6241 agctgtctta gcattcgctt gctgtgcgat atcgtataat cctaatgaag catcaccatt
     6301 tgctaaaaag tcatcgatga taagtacagt atcttcttct gataaaaact cttttgaaac
     6361 aatgaccgta cttgttttat ttttagtaaa tgaatgaata gatgtttcat aataaccatc
     6421 cgtcaaagtg ctaggttttg cttttttcgc aaataaacat ggcacatcaa aatgcagtgc
     6481 agccatgatt gcaggtgcga taccggaagc ttcaatggtt aagattttag taatcccttt
     6541 atctttaaat tgctcgtaaa aagtgcgacc aacttcattc attaactttg catcaatttg
     6601 atgatttaaa aatccatcga cttttaaaat cttctcatca atgacaacgc cgtcttcctt
     6661 tactttttgt cctagtaact ccactcttaa aacctcctca gatttgtgtg aaacattttg
     6721 ctcttaaatt ggtgctagat acaaaaaaat ccccaaacta aataatagtt tcagggttta
     6781 tgagtgaacg aacatgcata acgaatttgt catgcaatca atgtaaaaga agtttcatca
     6841 aataaactgt gaccatcata taaaatgata taaatcaccc accatggtta caatttaatg
     6901 gctgaagcta ctcctagtat tgtgttgtta ctcatagtca tgtcgttcaa ggcaacatgg
     6961 tagaaacttc taaagccata ttctttagat tatatgagtt tatgtaaatt atttaacgat
     7021 aatagcaaat tttcggcatt ttttcaataa ctgcttaggt aatcttttaa tagttttata
     7081 aatgtacaga gacgtttaat gcgaatgaag acttataact taataatgta agggttttac
     7141 tttataataa gaagggtaaa agattaaata aacaaatttt taaaggggcg acaactatgg
     7201 cagatattac tgtagtaaat gacactggcg aattatacaa cgtaatcaat caaaagaaat
     7261 ctgaaggtta tttagaatca gagctaacaa ttattagtaa aagtaaatta cacctaaatg
     7321 acttacacga ctctgaaatc tcccttatct caacaagtgg aacatttagt gatagaatga
     7381 ctaaactgtt aacaggagaa gatggtgaac atgcagtttt atcacgctac aacttagcgc
     7441 ctgatgaatt agaaaaatat aaacaactta ttttagacga taaaatgtta gttgtagcgg
     7501 ttcgtgacaa atcatcacat caagaagtgc atgaaaataa ttcagcttat gaagagattg
     7561 atattacaca ttttgctgaa gcatctaaag gtcccaaagc ttagttatca cactgaattc
     7621 aaaatgagat tactgtactg tctaatttaa gatagtacag ttttttatac catttaaaag
     7681 tttatgaaca gtcttttaca gcaggcgatt atgctataat attatttaaa ttggggaggt
     7741 gttcttatgg aatttaaaac cattcaatct gtcaaagatc cacattacaa cgaagcactt
     7801 aaactttacg atactaagtt aaatttatct ttaactgaag ataaacgtat catcaaacgc
     7861 tcattagaaa acaacaaaac cgaaaacgat tatgtcttta tcgtaggctt agaaaacgat
     7921 gttgttgtca gtctagcaac tgcacattat gaagccacaa ccaattcagc atttttaatt
     7981 tatttattcg caaaagacct acccaaccat gatgagttaa tgacgcaaac attacatcaa
     8041 attgaacatg aactaaactt attatcaaac cttgttcatt ctagaagtat taacttttta
     8101 atgatggaag tccctaaaga acctgcagat ttaacacaag ataacggcct cattattgaa
     8161 caccgtagac agtttttata cgaacatcaa tttgaaagac aaactgaaat tgattacatt
     8221 catccaacta atgatggtca cactaaaaaa gtggacttat ttttaaagaa aaatattaaa
     8281 ctgacaaagg atatctacgg tacaagtgtt aaatcaaatt atattttgaa gtatgtattt
     8341 gccaatggta tcgcacgtga aattatttat gctttattag aagaaatgga tttacgtaaa
     8401 ccattttaaa caaacttgaa atcactaaca ataacgttta aactatgtat gaagcaagac
     8461 tgacgtttca ctgcccgtag acaaacgtca gtatacataa agtatatgca gaaaggactt
     8521 taaagtatgt taactaaaga atttgcacaa cgcgtagaac taagtgagaa gcaagtacgt
     8581 aaaattgttc aacatttaga agaacgtggt taccaactaa gcaagactga atatcgtggt
     8641 cgtgaagcaa ctgatttcaa agaagaagat atcgagcttt tcaaagacat tgccgacaaa
     8701 gtaaaacaaa caaatagtta tgatctagcg tttgatgaat tagaaaaaga aaaagacttc
     8761 ctgcaagtca ttgtcaaaaa cgatgacaaa aacttaccta ctaatcaaaa tgtcgctcaa
     8821 ctagtagaag atttacgcct agaaatccag aaaatgcgcg aagaacgtca cctacttggt
     8881 caaatgatga atcaagtaca tcagcaacaa caagaattaa aagaacttca aaatcaactt
     8941 acatctaaaa tcgattcaaa tagcgaatcc ttaaaagcca tccaaacatc acaagaggct
     9001 atccaagaag cgcaagcctc tcaagcaaaa gcattagctg aatcaacaaa taaagttgaa
     9061 aagaatgctg ttactgaaga taaagctgac agtaaagatt cgaaggttgc tggagtgaat
     9121 acatcaacag acgctaagac ggatactaaa gcagaaaatg caggcgatgg cactgctact
     9181 aaagtagata aagaggatca aatttctgca actgaagcaa ttgaaaaagc cagcgttgag
     9241 cagtctaaaa atgacaatgc agctgagact tcaaataaag aagctacagt agatgcagaa
     9301 gcgcaacatg atgctgaaca acaagtagca gaagcccatg cagaagcttc taaacaagct
     9361 acttcgaacg attcattgga agcgaaagca gaaaatgaca gcacagcttc acagtcagaa
     9421 atgtcagaac caaaacctca agaagagaaa aaaggtttct tcgcacgttt attcaacctg
     9481 taaacttgtg atttgaacac aaatttaata ctcaattcat atatagcttt gatttaaaaa
     9541 ctgattttta taaacctata gaactgtaat ttactttcaa atttaatact caattcataa
     9601 aacctgaaac aagctgatat actccattca atatctactt tgaagagagc atatcaaact
     9661 aatttgtttc aggtttttta attattcatt atgattaata ttgaattttg aataaatttt
     9721 tatagtatgt tacttttaaa tgggatggct tctgtgtact tctaaataga ttcatcttaa
     9781 taagtccatt caatttaaat gtttggctgt gttagtaact gacgaatcgc acaagtgcac
     9841 actcatattc acatttacta caaacgcctt tttgctcaaa caaaaatatt tgtttacggt
     9901 agcttttgaa ctaccacctg cacacacgaa cactcaaaag atgctaattt tgattcgaca
     9961 gaatttttta caacgtgttt gttcgaaacg tcttttcaag gttgatcatt tattatcttt
    10021 tcattcaata aatatgaggt tagcaatttt tatcaaaccg ccatagccac atctcgattc
    10081 aaggataaat ttgttttctc atatcttatc tctcatgaca gtgcacgata tatatctttt
    10141 ccaaatattc atcacttttt tcctgttaaa atcatttcta ttattaatgc tttaatgaca
    10201 acttatttaa tttattgcat ttaatgccct gcttcatttt caaatattaa ctttaatcga
    10261 ctagctcatc acactattta ccctttccac ctatcactac tgaattcaaa acgatttgtt
    10321 tacaaatccc cttatgccaa cggtcgaatt tcatcatgtt tacatttaat tgcaaatgcc
    10381 ctcttgaaat attttaaaat taatattatt cttagcatac tagtcggaat tatattcaaa
    10441 aatttgagga gtgacgttca tgaatgcttt tcttacactg attaatatta ttgtattggt
    10501 gattttcatc gtaatattgc acatgatggc tcgcaaacat atatcttttg cgaaacgtgt
    10561 gtttacagct ttagggattg gtatagtatt cggtgtgtta ttgcatctta tttatggcac
    10621 acattcgaat gttattacat caacaagtga ctggttcaac attgtcggac aaggttacgt
    10681 tgcattgttg caaatgattg ttatgccttt aattttcatt tctattgtag ctgcattcac
    10741 taaaattcaa atcggagaaa agtttgctaa aattggtagt ctcattttta tctttttaat
    10801 tggcactgtc accattgctg cgattgttgg tgttgtgtat gccttggtgt ttggtttaga
    10861 tgcatcaaca attaaccttg gtaacgctga acaagcacgt ggaagcgaaa ttgctaaaca
    10921 agcgaaagat ttaacagcac atacgttacc acaacaaatt ttggaattat tgcctaaaaa
    10981 tccattctta gatttcactg gacaacgtgc aacttccacg attgctgtcg tgatatttgc
    11041 ttcgtttata ggatttgctt atttacgcgt agcacgcaaa caacctgatc atggtgaatt
    11101 gcttaagcgt gcgattgacg ctatctattc actagtaatg gcgattgtga cgttcgtctt
    11161 acgtttaacg ccatacggtg tattagcaat tatggccaac acattatcga caagtgactt
    11221 tggcgccatt tggactttag gaaaattctt aatcgcatcg tacgctgcat taattacgat
    11281 gtatatcatt cacttaatta ttttaagcct gttaggtatt agcccaattc gctatgttaa
    11341 aaagactttg gaagtattaa tctttgcatt cacatcacgc tcaagcgcgg gtgccttacc
    11401 attgaatgta caaacacaaa caagacgtct aggcgtgcca gaaggtattg ccaatttcgc
    11461 tgcgacattt ggattgtcga ttggacaaaa tggttgtgca ggcatttacc ctgccatgct
    11521 tgcaattatg gtcgcacccg tcgctaatgt agaaattgat ttacaattta ttgtaacgtt
    11581 aatcgctgtc gtgattatta gttcattcgg cgttgctggc gtcggtggcg gggctacatt
    11641 tgcatccata ttggtgttgt caacgttaaa cttgccagtc gctttagccg gcgtgctcat
    11701 ctccgttgaa ccattgattg acatgggtag aacagcatta aacgtgaacg attcaatgct
    11761 agctggtaca ggtaccgcca agttaactaa acattgggat aaagatactt tcgattcaaa
    11821 tgataatgct gcattaactt cacactaatt cattgcaaga aatacccgcc ccctctagct
    11881 atacttatca ttttaagcta gaggggggcg ggttattttg gtatcttgct atcgctgtat
    11941 taagcctgat ttttgcaatt gttctaacat atctaatctt gctttgtttc ctaggaacat
    12001 ctcaatttgc tgcgaccatg tttctttgcg attcccgttt gtacgttgat catagtactc
    12061 gctgattgtc tggtcgtaat ctgccatttg tgcatactgt gtttccttat cagcatgata
    12121 cttattatga tggaagacat ggtcaaatgg taagcgtggc ttggctgcac cattttcgtc
    12181 atctgcgggt tcccctactg ccataccaaa taccgggaag acatagtcag gtaagtctaa
    12241 aatttctcga acgcgttcaa catcatttct taatgatcct aaaaagacaa tgccataccc
    12301 catatcttca gcagttaccg caatattttc ggcaactaat gctgcatcga ttgcacctac
    12361 tagcaaacct tccgttgaac catatgcatt ttccatatca gtttcagcat gttgatcaac
    12421 taaatgatga cgataataat caataacaaa gacgaataaa tagccatttt ctacaacata
    12481 aggttgtcca gaaacttctc gtaaattttc tttaatcttc tcatcgtcga taccaataat
    12541 tgagtatgct tgcaggaaac ttgacgtcga agcgctttgt ccagcttcta ccaatttctt
    12601 aacaacgtct tcacttaaag gtttattctt aaattttcta acagaatgat gctttttcac
    12661 aagattatat acatgttctg acacgttacc cactcctcga tactttacaa ttaatttaaa
    12721 atcttcatat tgcaattgta cacgtccaaa cttttttata gcaattattt gcttataagt
    12781 taataattga actttttaaa atattgtctc atactttaaa aatccagttt ctcactcatt
    12841 accttcatcc atctcgaaca aatattctca caccattatc aataaggttg acttagttga
    12901 gaatacaaat cttctttttt tacatttttt acattaaaat caagatatta aatatagaca
    12961 caacctttta aatcatcaac tacaaaatcc attatcaaag cactttatat tatctttctt
    13021 tattttaaaa gtaatcattc actttacaaa gttatccagt tgactattct ggatcaaaat
    13081 gtaataatgc taactaagag attagaatta ttataattaa gcgcaatcaa ttgatagttc
    13141 taattcatac ttaaattctt aggaggaaga tatttatgtc attaattaac aaagaaatct
    13201 taccatttac agcgcaagct ttcgatccaa aaaaagatca atttaaagaa gttacacaag
    13261 aagatttaaa aggttcttgg agcgtagtat gcttctatcc tgctgacttc tcattcgttt
    13321 gtccaactga attagaagac ttacaaaacc aatatgaaga attacaaaaa ttaggcgtaa
    13381 atgtattctc agtatcaact gatactcact tcgtacacaa agcatggcat gaccattcag
    13441 atgcaattag caaaatcact tacactatga ttggtgaccc atcacaaaca atcactcgta
    13501 attttgatgt attagatgaa gctactggtt tagctcaacg tggtacattc attatcgacc
    13561 cagacggtgt tgtacaagca tctgaaatta acgctgacgg aattggccgt gacgctagta
    13621 cattagctca caaaatcaaa gcagctcaat atgttcgtaa aaaccctggc gaagtatgcc
    13681 cagctaaatg ggaagaaggc gctaaaacat tgcaacctgg tttagattta gtaggtaaaa
    13741 tctaaggagg cattacaacc atgcttaatg ctgatttaaa acaacaactt aaacaactat
    13801 tagaactaat ggagggcaac gttgaattcg ttgccagcct tggttcagat gataaatcca
    13861 aagaacttaa agatttgttg acagaaatta ctgatatgtc acctagacta tctctttctg
    13921 aaaaatcttt aaaacgtaca ccaagtttct cagtcaatcg tcctggcgaa gaaacaggtg
    13981 taacatttgc aggtattcca ttaggtcacg agtttaactc acttgtttta gcaattttac
    14041 aggttagtgg tcgtgcacct aaagaaaaac agtcaatcat tgaccaaatt aaaaaattag
    14101 aaggttcatt ccattttgaa acattcatta gtttaacgtg tcaaaaatgt cctgatgtcg
    14161 ttcaagcact taacttaatg agtgtgatca accctaacat cacgcattct atgattgatg
    14221 gtgcagtgtt ccgtgaagaa tctgaaaaca tcatggcagt ccctgctgtc tttttaaatg
    14281 gcgaagaatt tggcaatggt cgtatgacaa tccaagatat tctttcgaaa ctaggcagta
    14341 cggcagatgc atctgagttt gaaaataaag aaccttatga tgtcttaatc gttggtggtg
    14401 gtcctgctag tggtagtgca gcgatttaca cagcacgtaa aggtttacgt actggtatag
    14461 ttgctgatcg tatcggtggc caagttaatg atactgctgg tattgagaac ttcattactg
    14521 ttaaagaaac aactggttct gaattttctt ctaacttagc agcgcacatt gatcaatatg
    14581 acattgatgc aatgacaggt atacgtgcta cagatatcga aaagactgac gaagcaatta
    14641 aagttacgtt agaaaacggt gctgtcttag aaagtaaaac agtcattatt gctactggtg
    14701 caggttggcg taagctaaac attccaggtg aagagcaatt gattaataaa ggtgttgcat
    14761 tctgccctca ctgtgacgga cctctatttg aaaataaaga cgtagcagtt atcggtggcg
    14821 gtaactctgg ggttgaagca gcaattgacc ttgctggtat cgttaatcat gttacattat
    14881 tcgaattcgc tagcgaatta aaagcagaca acgtgttaca agatcgttta cgttctttat
    14941 caaatgttga tatcaaaaca aatgccaaaa ctactgaagt tgtcggagaa gaccatgtta
    15001 caggtatacg ttacgaagac atgaacaccg gcgaagaaca tctacttaac ttagatggta
    15061 tctttgttca aattggttta cttccaaaca catcatggtt aaacgatgct gttgaattaa
    15121 acgaacgtgg tgaaattgtg attgatcgta acaataatac gaatgttcct ggaatatttg
    15181 ctgctggcga tgtcacagat cagaagaaca aacaaattat catttcaatg ggcgctggtg
    15241 caaatgcagc attaaatgcc tttgactata ttatcagaaa ctaaagtttc attcatttca
    15301 ctgacagtat cttacatttg ttaagatgct gttatttttt tatcccgaca taaaatcatg
    15361 ttatgataaa cttaatatat acaacgagat gtgcgataat gcttaaagta tgtacctctc
    15421 gcaaacaaat caatatgaaa aatgatattg agaaagaaaa gtatgtgaac gaaattatta
    15481 tgcttttacg tacgatacct acgtctaaat tagtacaacc aattatgcag ttatttatag
    15541 ataaagctat ccttaacaac gtccaaaatg atgcattatt gatagaaaaa gcaactcatg
    15601 cacgtaatca tttacgtcta tggtcattgc aaggtcatcg tgacattcca aactaattca
    15661 atgttactct attaaacatg aatacatatt ttttcaatta aagataaact aaggagttat
    15721 attacattat gaaaaagaga ttactactaa gtacattttt agcatcgaca ttaattctta
    15781 caggttgcgc gtccgatcaa tctgataacg aagatcatca tacaagtact ggcatccatg
    15841 cacctaaaag cgctaaaaaa ttagaaacaa aagatatctt taactcagat aagaaaaata
    15901 gtgatattag tgatgcagag atgaagcaag ctatagaaaa gtatttgtct gtaaatagcg
    15961 atatacttga taacaaatat attatgcaac ataaactaga taaacaaatt gacagtcaaa
    16021 caaaagtgac tgaaaaacaa gcagaaacac ttagccactt atctaactta gctgttaaaa
    16081 acgatttgca tttcaagaaa tttgtcactg aaaacaatat tcctaaagaa tataagaaac
    16141 ccgttgaatt aatgatgaat tacttcaaag ctttaaatag tacaatcgcc aatgttgatg
    16201 aagatattga aaagcttagc tatcaaccgc aaaataaaat caatgttgtc gacgtgccaa
    16261 caaaatatgc cggtgatgtt aataaaaagc aacaagataa aattaaagat ttcttaaaat
    16321 ctaaaggtat caagagtgat gttatcgata aataactgaa agccatcatt gcgaaatgta
    16381 acacatcatt acatgagttt taattcatga caattcttta tctttaaaag tgactaatca
    16441 atttagctaa agtcatcatt tgggcatcaa gcagcatatt actgtttgat gcctttttaa
    16501 ttgccttaaa ttctctatct atgccaaaat tcccattagt tgacgcattt gactatatac
    16561 ctaacatatt ccatacttat attgactcta taaacaaagg atgatcatga tgaaagttcg
    16621 aattactttt attattttag caattttatc gacgattgtt tgcttgttct tagcagcaat
    16681 gcatccaaca ggaccaaaca cagtaacttt tgaacagcca tatttattca ccctcaacat
    16741 cattataatg gtattagtcg cattaccttc acttgtttta gctatatatg attatatgag
    16801 ttttagaatt tcttctgcta ttttacaatt tctaggggct atctcttggt tctttttatc
    16861 attgatatta tcgctcacac aatttacacc ttttacatta gcgtcattta taacttcaat
    16921 tattttgttc acaagcacaa ttatcacatt agccattggt ggtaagtctg ttgaaaagaa
    16981 tgattcccct taaattccaa atgaaaaaaa ggttctgaag gccgctataa aacacagttt
    17041 ttcagaacct ctatacttct attcaatgat atatggtttg caattttcta cctttaaatc
    17101 cacagcttct gcccttgaaa ctttgttaaa ataaaccatc aaacaacgaa tgacaacttg
    17161 atgtgcaaca atgacaatat catctttttg tgtatcttca ttgacaacat gattcataaa
    17221 atgttctacg cgttgatata catcttcata actttctcct tcaggcgctt tttgtgaaaa
    17281 actatgacga aagtctttaa agtttggatc attgaaatat ttatcatatt tcggattcgc
    17341 actgatttca tctttatatt caccctcaaa tacgccaagt gaacgttctc ttaatagagg
    17401 ggtagtcgtt gatgcaatgt catatggaaa aatatgttca aacgtttgct gtgttcttaa
    17461 taagtctgaa acatatacat gtttaatctg tttctcttta aaataatcac ataaatcgtc
    17521 tgcacttttt gtgccagtat ccgttaacgg cacatctaat tgtccacaaa aataagatcg
    17581 aaaatgttta ttatcataat tcgattttga ttcgccatgt ctaactaaat aaatcgtcat
    17641 aatattactc cttaccttat gtatttcata tctaccataa cactttgact actaattcga
    17701 tatcaatctt aatattctat tctaaaaaaa gaattaattc atattttaaa ttaatgactc
    17761 taaaaatagt attgtcttta atcaagtgat gtgttactat atgggtgtag ttcttaaatg
    17821 actacacagt cttttatatt catgaacata atactcctgg atggcgcaac ccaacgtcat
    17881 ccttttttta tttgtcaaat actcgctttt ttatttccaa aatttcctca tattaattta
    17941 ttacttttta atcctatctt atctacattt tcaaatatta taatacatat tgaccatttt
    18001 cacgcaaata aaaaagatga caactgaatg ccatctttat ttcaatttcc aattgaattt
    18061 ttttattatt tacgcatagc tcttaaaatt aacgttacga ttgcaattaa gataattgaa
    18121 ccaattaatg ctggcaagat gtaaatactt cctaattcag gaccccattg tccgaatagt
    18181 ttgccaccta cccatgatcc aataatacct gcgataatat tgcctaaaat accacctggg
    18241 atatctttac ccataatagc accagcagcc catccaatta agccaccgac aattaacatt
    18301 ccaataaatc caaacataat tttcagtctc ctttttctat ttattttgcg ttattctaag
    18361 tagtacccct tatttacaat tctaaaacaa attcaaatta tttttatcca aatattttta
    18421 aaagtagtaa ttgaatatca attttattca atgtagctat cgttatttaa agtctctgta
    18481 ccgataatat catatacatt tacattattt tttctgccga attcatagct tgattatttt
    18541 atgttatagg actagaatat acacatatta ttagagcatc tttgaatttt aaatcaagaa
    18601 gcgaggttaa tgaacaatga atatgcatat tttatataac ttacgaacta aacataattt
    18661 agaaattgac gaattagcac agcaattaaa tgagaaatat ggtactaaat atgaagcaca
    18721 tcaaatttgg gaatgggaga atcatcacca tgaacctaaa tttaaagatg ccatgcattt
    18781 agctgacttc tttgatgcac catatgaaat gtttttagaa agtaaggtta aagaatatca
    18841 gaaacattta gaagaagtcg atattcgcat ggataaatag atgcaaataa accctcacaa
    18901 cacgtttggc atatatcctt tcaaatctat actggatata ttacattacg ttgtgagggt
    18961 attttattaa ttaatatgaa ttaagacatt ttacaagcgt taatgcaacg aatcttttta
    19021 gtgatcttgc tcactcttta atactttacc gttcttagca tcaacagtaa cttcttgttt
    19081 tttattacct tttttcaaat cgatattgta aacaagtttg ccatcatctt tttcaagtga
    19141 ccattcttta atatcaccat caaattcttt ttgtccttct ttaatggctt ttttgtaatc
    19201 tatagcatcg ctatatttaa agttatcatt ttcattcatt gtatcttctt tttcagtctt
    19261 tttgttaatc acttttttat ttttatcagc aacaagtact tctgactctt caccagattt
    19321 ttgttgcgtc actttataag cccattcacc attagaattt tcaaatgaaa ttcctttcaa
    19381 cttttggcct ttgtaagttt cttcagcttt tttcacagca tcttctgggc ttgttttaac
    19441 atcttttaaa gcaataacat cttttgttgt attagtgtct tgattagtat ttgactctgt
    19501 tgattttgtt tcatcttttg gagtatcatt gccacatgca gtaagcacca ccgctgacat
    19561 tgataacact gctaatgatt ttaatttcat aatatcactc tctcttccta tttttgaaac
    19621 tcataacaaa agcttatatg ctatatagat tgtattaccc cttgttttta attttattca
    19681 taattattac aaatattttt aaattaatcg tcatgcgtta ctttcgttcg tactcctttt
    19741 ataaatgaac catgtaatat aagcatgcta ttatcgactc agtttgtcta atgctttctt
    19801 tggtacttct tccttttcaa cttcttcaaa agtttctaca tgatgacctt tatgtgtaat
    19861 tttcaagtat ctatgcggtt taacatcaaa tgtagcagta tacattaatt gcgtctcttt
    19921 cccttcttta ttaaaaacac tttgactata acttcggagt tgatcatcca ttctagtaga
    19981 cacctctgtc gttttaacat aagaatcatc cttctttact aatgggttaa attgatctgt
    20041 tataccatgg gagtctattg tttttaaaat gaataaagca gcataagcgc caatgatagt
    20101 aagtacaaga tatgttattg tttttaaaat cgttttcaaa agatcacctc tgacaaaata
    20161 taaaatagga tgtatctgaa aacaacttac ataattgtaa ttaaagtgta agtattagga
    20221 gaaaatgatg gtatttgaat ctgaaagata cgaatcgttt ttaaactaag ttattttata
    20281 gagactttat tatgaaattg aaagaagtta attttaaggt tgtcgaatgt aaaaacatat
    20341 tgattaacca taaaagagaa attatttttg tagatttcgg cataaacgtc attaaagaat
    20401 tttctatatg atggtgctaa tcataacaaa gaaagctagg cacctttaga tacctaactt
    20461 tcaaagtatt agcgataaaa cactatatat actaatttag cttaatttca aacttgttca
    20521 atgtcattaa cacttttcac aagtttgaaa gttacatttt ttatatcggc tgtattaatt
    20581 tttacatttt ccctatcttt ttgtagctct ttatgcgact ctaatgtata tttaccacca
    20641 tcttttgtat ttatgacgat gttaccttta ggcaaatgtt ctcccatata caagccatag
    20701 gaaatatgag caaatcttat gatatgatcc aattctttta aagtgacaac ttctttatta
    20761 agagtaatat tcctcttagg tgtatacact ctgccagcaa ctgtgtatgt tccttctaaa
    20821 tgtatacgaa cttggttttc atttaaaggt ctctctgaat aaacccaatt cctagattta
    20881 ttagattttc catttattgt atccttgctg tatctatcgt aaagtttttt aaccaaagct
    20941 ttttctgaat tatttggtac ttcttcaact ttttgtaatt gtttatcttg tacacttgag
    21001 gaattttgtg ttgacgcgtc tgcttttgga tatattccaa atgttccccc atagatgata
    21061 cctagcgata gaatagattt taatacgatt gaattttttg agtcatattt tttgaacata
    21121 tttaattacc tccttgatgt aaagctttat ttgctacaat tataaaaata atagacgtgt
    21181 tcatgaatta aattcatctt aactcttgat taactttaat ttgctaccac tctgaattta
    21241 ataactataa atcgtctaca cataattgga caaaatctaa gagaataaaa tttgttaatt
    21301 taaaatagca agcaattcaa agttatatgt gtaatagata aaatagatat ccctatagtg
    21361 atgcgttact agctaaacat aataacacat tagaagataa tgaagttaag gagttactgg
    21421 attgtttcga ctatgtaatt aagtataaaa atatccaacg acaaaacgta attataaaat
    21481 ggtaaaagct atggtacagt ttcaaattgc taatgacatg cgtatcggtg agctacttgc
    21541 aataaagaga gtaaatataa actatgaaga taaaacgcta gatatcgacg gtaaagttaa
    21601 ttggataact gaaaaaagac gggagcattc ggagtaaagg agacaactga aagaagtaat
    21661 agctataagg ccacagggct cactacccaa agcatcgact tactaagaac acttatgctt
    21721 ggaatgataa gtttattgat agagagtaca tattcacaaa tacgtctggt agccctatcg
    21781 actcgaacaa aattagccac attattaaag gggggcgctg atattagttc tattaagaaa
    21841 cctataacga cgcatacatt acatcattcg catatatcta cacttgctca attaggaatt
    21901 aacttaaaag caatgcaaga gcatgtaggt cattcagatt ataaaaaaaa tctagagata
    21961 tacacacatg ttactaatca gatggcgaaa gatatgatga ataaatttga acgattgggg
    22021 agttaaaatt ggaaaaagat gatacactag cagaaattaa gcctatgctc aattttgatg
    22081 agcaaatagc gaaattaaaa cagatgaata tattttttaa tattattgac accgaaaaag
    22141 caaatgaaat tcttagaaaa aataattact tcttcaaact agcttatttc cgaaaaaatt
    22201 tcgaaaaaaa gaatggcggc tatttcatag aatttgctta tttatcagat ttagcaacta
    22261 t
//
LOCUS       DAMQYT010000021        21228 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMQYT010000021 DAMQYT010000000
VERSION     DAMQYT010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21228
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.021"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..302
                     /locus_tag="PGA33_000677"
     CDS             <1..302
                     /locus_tag="PGA33_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HDG6472510.1"
                     /translation="LTKKQLKEYIEDYKKSDDILINLYIETYEFYCRLRDELKNSDLM
                     IEHTNKAGASNIVKNPLSIELTKTVQTLNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            292..1983
                     /locus_tag="PGA33_000678"
     CDS             292..1983
                     /locus_tag="PGA33_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDG6472511.1"
                     /translation="MTIKVLNEPSPKLLTTWYAEQVTQGKIKTSKYVKKECERHLRYL
                     ENGGKWVFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLR
                     RFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMI
                     KASPKLDKNFRTLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLI
                     SVIKNSRAARLQPLLIYITTAGYQLDGPLVDMVEAGRDTLDQIIEDERTFYYLASLDD
                     DDDINDSSNWIKANPNLGVSINLDEMKEEWEKAKRTPAERGDFITKRFNIFANNDEMS
                     FIDYPTLQKNNEIVSLEELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLSHSWI
                     PKHKVEYSNEKIPYREWEEDGLLTVQDKPYIDYQDVLNWIIKMNEHYVVEKITYDRAN
                     AFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQL
                     KLDRNGNWLPSKQSRYRKIDGFAAFLNTYTDIMNKVVSDKGEGNIEFISIKDIMR"
     gene            1988..3226
                     /locus_tag="PGA33_000679"
     CDS             1988..3226
                     /locus_tag="PGA33_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDG6472512.1"
                     /translation="MNVIAKENIVTRIKKKLIDNWIDQSASKLYDFSPWKNKSFWGVI
                     NNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFIN
                     QIETIRNEKGNAYVLIERDIYHQPSKLFLLNPDVVEMLIENQSRELYYSIHAATGNKL
                     IVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGS
                     NVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVAN
                     VFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRY
                     FKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPI
                     DTPLELRKSLKGGDKNVNES"
     gene            3210..3983
                     /locus_tag="PGA33_000680"
     CDS             3210..3983
                     /locus_tag="PGA33_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDG6472513.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLEKTDAVSNSAYLDKAKDLDQEHLKQ
                     MLDAETWLTAEEALSFGLIDEILGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMNY"
     gene            3995..5158
                     /locus_tag="PGA33_000681"
     CDS             3995..5158
                     /locus_tag="PGA33_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDG6472514.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVKKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGPLPS"
     gene            5227..5505
                     /locus_tag="PGA33_000682"
     CDS             5227..5505
                     /locus_tag="PGA33_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDG6472515.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            5517..5849
                     /locus_tag="PGA33_000683"
     CDS             5517..5849
                     /locus_tag="PGA33_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472516.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIVRSSKTEYLPQTNHLVKIDSALYSDKLFNIVEIRIDTPDIGY
                     NTVVLSEK"
     gene            5846..6247
                     /locus_tag="PGA33_000684"
     CDS             5846..6247
                     /locus_tag="PGA33_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472517.1"
                     /translation="MSVEIKGIPEVLNKLESVYGKQAMQAKSDKALNEASEFFIKALK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAASERKYREIIKKELAR"
     gene            6248..6643
                     /locus_tag="PGA33_000685"
     CDS             6248..6643
                     /locus_tag="PGA33_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HDG6472518.1"
                     /translation="MNILNTIKGILLSDAELKTHINSRIYYYKVTENAETSKPFVVIT
                     PVYDLPSDFMSDKYLSEEYLIQIDVESSNNQKTIDITKRIRYLLYQQNLIQASSQLDA
                     YFEETKRYVMSRRYQGIPKNIYYKNQRIE"
     gene            6678..7319
                     /locus_tag="PGA33_000686"
     CDS             6678..7319
                     /locus_tag="PGA33_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDG6472519.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSDDLNTVIGRTKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDSEDHSEDSAGSLPS"
     gene            7411..7866
                     /locus_tag="PGA33_000687"
     CDS             7411..7866
                     /locus_tag="PGA33_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDG6472520.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVA
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILK
                     YTSEHPEFVTVDENTGAIHGVAEGTSVITAMSTDGSDKSGQISVTVTNG"
     gene            7924..8274
                     /locus_tag="PGA33_000688"
     CDS             7924..8274
                     /locus_tag="PGA33_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472521.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALQDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            8316..8474
                     /locus_tag="PGA33_000689"
     CDS             8316..8474
                     /locus_tag="PGA33_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472522.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            8488..14688
                     /locus_tag="PGA33_000690"
     CDS             8488..14688
                     /locus_tag="PGA33_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDG6472523.1"
                     /translation="MNEKVEGMTLELKLDHLGVQECMKGLKRQLGVVNSEMKANLSAF
                     DKSEKSMEKYQARIKGLNDRLKVQKKMYSQVEDELKQVNANYQKAKSSVKDVEKAYLK
                     LVEANKKEKLALDKSKEALKSSNTELKKAENQYKRTNQRKQDAYQKLKQLRDAEQKLK
                     NSNQATTAQLKRASDAVQKQSAKHKALVEQYKQEGNQVQKLKVQNDNLSKSNDKIESS
                     YAKTNTKLKQTEKEFNDLNNTIKNHSANVAKAETAVNKEKAALNNLERSIDKASSEMK
                     TFNKEQMIAQSHFGKLASQADVMSKKFSSIGDKMTSLGRTMTMGVSTPITLGLGAALK
                     TSADFEGQMSRVGAIAQASSKDLKSMSNQAVDLGAKTSKSANEVAKGMEELAALGFNA
                     KQTMEAMPGVISAAEASGAEMATTATVMASAINSFGLKASDANHVADLLARSANDSAA
                     DIQYMGDALKYAGTPAKALGVSIEDTSAAIEVLSNSGLEGSQAGTALRASFIRLANPS
                     KNTAKEMKKLGIHLSDAKGQFVGMGELIRQFQDNMKGMTREQKLATVATIVGTEAASG
                     FLALIEAGPDKINSYSKSLKNSNGESKKAADLMKDNLKGALEQLGGAFESLAIEVGKD
                     LTPMIRAGAEGLTKLVDGFTHLPGWVRKASVGLALFGAAIGPAVLAGGLLIRTVGSAA
                     KGYASLNRRIAENTILSNTNSKAMKSLGLQTLFLGSTTGKTSKGFKGLAGAMMFNLKP
                     INVLKNSAKLAILPFKLLKNGLGLAAKSLFAVSGGARFAGVALRFLTGPIGATITAIT
                     IAYKVFKTAYDRVEWFRNGINGLGETIKFFGGKIIGGAVRKLGEFKNYLGSIGKSFKE
                     KFSKDMKDGYKSLSDDDLLKVGVNKFKGFMQTMGTASKKASDTVKVLGKGVSKETEKA
                     LEKYVHYSEENSRIMEKVRLNSGQISEDKAKKLLKIETDLSNNLIAEIEKRNKKELEK
                     TQELIDKYSAFDEQEKQNILTRTKEKNDLRIKKEQELNQKIKELKEKALSDGQISENE
                     RKEIEKLENQRRDITVKELSKTEKEQERILVRMQRNRNAYSIDEASKAIKEAEKARKA
                     RKKEVDKQYEDDVIAIKNNVNLSKSEKDKLLAIADQRHKDEVRKAKSKKDAVVDVVKK
                     QNKDIDKEMDLSSGRVYKNTEKWWNGLKSWWSNFREDQKKKSDKYAKEQEETARRNRE
                     NIKKWFGNAWDGVKSKTGEAFSKMGRNANHFGGEMKKMWSGIKGIPSKLSSGWSSAKS
                     SVGYHTKAIANSTGKWFGKAWQSVKSTTGSIYNQTKQKYSDASDKAWAHSKSIWRGTS
                     KWFSNAYKSAKGWLTDMANKSRAKWDNISSTAWSNAKSVWKGTSKWFSNSYKSLKDWT
                     GDMYSRAHDRFDAISSSAWSNAKSVFNGFRKWLSKTYDWIRDIGKDMGRAAADLGKNV
                     ANKAIGGLNSMIGGINKISKAITDKNLIKPIPTLSTGTLAGKGVATDNSGALTQPTFA
                     VLNDRGSGNAPGGGVQEVIHRADGTFHAPQGRDVVVPLGVGDSVINANDTLKLQRMGV
                     LPKFHGGTKKKDWLDQLKGNIGKKAGEFGATAKNTAHNIKKGAEEMVEAAGDKIKDGA
                     SWLGDKIGDVWDYVQHPGKLVNKVMSGLNINFGGGANATVKIAKGAYSLLKKKLVDKV
                     KSWFEDFGGGGDGSYLFEYPIWQRFGRYTGGLNFNGGRHYGIDFGMPSGTNVYAVKGG
                     IADKVWTDYGGGNSIQIKTGANEWNWYMHLSKQLARQGQRIKAGQLIGKSGATGNFVR
                     GAHLHFQLMQGSHPGNDTAKDPEKWLKSLKGSGVRSGSGVNKAASAWAGDIRRAAKRM
                     GVNVTSGDVGNIISLIQHESGGNAGITQSSALRDINVLQGNPAKGLLQYIPQTFRHYA
                     VRGHNNIYSGYDQLLAFFNNRYWRSQFNPRGGWSPSGPRRYANGGLITKHQLAEVGEG
                     DKQEMVIPLTRRKRAIQLTEQVMRIIGMDGKPNNITVNNDTSTVEKLLKQIVMLSDKG
                     NKLTDALIQTVSSQDNNLGSNDAIRGLEKILSKQSGHRANANNYMGGLTN"
     gene            14688..15512
                     /locus_tag="PGA33_000691"
     CDS             14688..15512
                     /locus_tag="PGA33_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDG6472524.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNASGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            15521..17104
                     /locus_tag="PGA33_000692"
     CDS             15521..17104
                     /locus_tag="PGA33_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDG6472525.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKTPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIRTTDIKYSNGFIKEGTYRTETIGSKATINFDCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSETIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            17104..17394
                     /locus_tag="PGA33_000693"
     CDS             17104..17394
                     /locus_tag="PGA33_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472526.1"
                     /translation="MATEEVKIKALLENDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDKLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            17410..19320
                     /locus_tag="PGA33_000694"
     CDS             17410..19320
                     /locus_tag="PGA33_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472527.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTMQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSLIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFPTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
     gene            19320..20786
                     /locus_tag="PGA33_000695"
     CDS             19320..20786
                     /locus_tag="PGA33_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDG6472528.1"
                     /translation="MSNLEKSVAINLENTAHYENISNLDITFRTGESDSSVLLFNIIK
                     NNQPLLLSEENIKARIAIRGKGVMVVAPLEILDPFKGILKFQLPNDVIKRDGSYQAQV
                     SVAELGNSDVVVVERTITFNVEKSLFSKIPSETKLHYIVEFQELEKTIMDRAKAMDEA
                     IKNGEDYASLIEKAKEKGLSDIQIAKSSSIDELKQLANSRISDLENKAQAYSRTFDEQ
                     KRYMDEKHEAFKQSVNSGGLVTSGSTSNWQKAKITKDDGKIMQITGFDFNNPEQRIGD
                     STQFIYVSQAINYPRDVSTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEAGSWSEW
                     SELAINDYNTPFETVQSAQSKANMAESNAKLYADDKFNKRYSVIFDGTANGVGSTLYL
                     NESLDQFILLIFYGTFPGGDFTEFGSPFGGGKISLNPSNLPDGDGNGGGVYEFGLTKS
                     SRTSLTISNDVYFDLGSQRGSGANANRGTINKIIGVRK"
     gene            20786..21175
                     /locus_tag="PGA33_000696"
     CDS             20786..21175
                     /locus_tag="PGA33_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HDG6472529.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQLVTLNKKLEEVKGETENA"
     gene            21168..>21228
                     /locus_tag="PGA33_000697"
     CDS             21168..>21228
                     /locus_tag="PGA33_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDG6472530.1"
                     /translation="MLKLISPTFEDIKTWYQLKE"
BASE COUNT         8144 a         2902 c         4279 g         5903 t
ORIGIN      
        1 aattaacaaa aaaacagctg aaagaatata tagaggatta taaaaaatct gatgacatat
       61 taattaattt gtatatagaa acgtatgaat tttattgtcg gttaagagat gaacttaaaa
      121 atagtgattt gatgatagag catacaaaca aggctggtgc gagcaatatt gttaagaatc
      181 cattaagcat agaactgaca aaaacagttc aaacactaaa taacttactc aagtctatgg
      241 gtttaactgc agcacaaaga aaaaagatag ttcaagaaga aggtggattc ggtgactatt
      301 aaagttttaa atgaaccttc accaaaacta ttaacaacat ggtatgcaga gcaagtcact
      361 caagggaaaa taaaaacaag caaatatgtt aaaaaagaat gtgagagaca ccttagatat
      421 ctagaaaatg gaggtaaatg ggtatttgat gaagaattag cgcaccgtcc tattcgattc
      481 atagaaaagt tttgtaaacc ttccaaagga tctaaacgtc aacttgtatt acaaccatgg
      541 caacatttta ttattggcag tttgtttggt tgggttcata aagaaacaaa actgcgcagg
      601 tttaaagaag ctttgatatt tatggggcga aaaaatggta aaacaactac tatatctggt
      661 gttgctaact atgctgtttc tcaagatgga gaaaacggcg ctgaaatcca tcttttagca
      721 aacgtaatga aacaagctag aatattattc gatgaatcta aggcgatgat aaaagctagc
      781 ccaaagcttg ataaaaattt cagaacatta agagatgaaa tccattatga cgcaacgata
      841 tcaaaaatta tgccccaagc atcagatagc gataagttag atggattgaa tacacacatg
      901 gggatttttg atgaaattca tgaatttaaa gactataaat tgatttcagt tataaaaaac
      961 tcaagagctg caaggttaca acctcttctc atctacatta cgacagcagg gtatcaatta
     1021 gatggtccac ttgttgatat ggtagaagcg ggaagagaca ccttagatca aatcatagaa
     1081 gacgaaagaa ctttttatta tttagcatct ttggatgatg acgatgatat taatgattcg
     1141 tcgaactgga taaaagcaaa tcccaactta ggtgtctcta taaatttaga tgagatgaaa
     1201 gaagagtggg aaaaagctaa gagaacacca gctgaacgtg gagattttat aaccaaaagg
     1261 tttaatatct ttgctaataa tgacgagatg agttttattg attacccaac actccaaaaa
     1321 aataatgaaa ttgtttcttt agaagagctg gaaggcagac catgcacgat tggttatgat
     1381 ttatcagaaa cagaggactt tacagccgcg tgtgctactt ttgcgttaga taatggtaaa
     1441 gttgcagttt tatcgcattc atggattcct aagcacaaag ttgaatattc taacgaaaaa
     1501 ataccctata gagaatggga agaagatggc ttattaacag tgcaagataa gccttatatt
     1561 gactaccaag atgttttaaa ttggataatt aagatgaatg agcattatgt agtagaaaaa
     1621 attacttatg atagagcgaa cgcattcaaa ctaaatcaag agttaaaaaa ttacgggttt
     1681 gaaacggaag aaacaagaca aggagctttg accttgagcc ctgcattgaa ggatttaaaa
     1741 gaaatgtttt tagatgggaa aataatattt aataataatc ctttaatgaa atggtatatc
     1801 aataatgttc agttgaaact agacagaaac ggaaactggt tgccgtctaa gcaaagcaga
     1861 tatcgtaaaa tagatggctt tgcagcattt ttaaacacat atacagatat tatgaataaa
     1921 gttgtttctg acaagggtga aggaaacata gaatttatta gtattaaaga tataatgcgt
     1981 taaggaggtg aatgttatcg caaaagagaa tattgtcaca cgcataaaga aaaaattgat
     2041 agacaattgg attgatcagt cagcttctaa gctttatgac tttagcccat ggaaaaataa
     2101 atctttttgg ggtgtaatca ataatacgct tgaaactaat gaaacgatat tttcagctat
     2161 tacaaagtta tctaattcga tggctagttt gcccttgaaa atgtatgaag attataaagt
     2221 agttaataca gaagtatctg atttacttac agtgtcaccg aataattctc tgagcagttt
     2281 tgattttatt aatcaaattg aaacaatcag aaatgaaaaa ggtaatgcat atgtgctaat
     2341 tgaacgagac atctatcatc aaccatcaaa gcttttctta ttaaatccag atgttgttga
     2401 aatgttaatt gaaaaccaat cacgtgaact ttattattcc attcatgctg caactggaaa
     2461 taaattgatt gttcataata tggacatgtt gcattttaaa cacatcgtgg catctaatat
     2521 ggtgcaaggc attagtccga ttgatgtgtt gaagaataca actgattttg ataatgcagt
     2581 aagaaccttt aatcttacag aaatgcaaaa acctgattct ttcatgctta aatatggttc
     2641 caatgtaggt aaagaaaaaa ggcagcaagt gttagaagat ttcaaacagt actatgaaga
     2701 aaacggtgga atattattcc aagagcctgg tgttgaaatc gaaccgttac ctaaaaaata
     2761 tgtctctgaa gatatagtgg caagcgagaa tttaacaaga gaaagagtag ctaacgtttt
     2821 tcaattgccc tcagtattct taaatgcaag atcaaataca aatttcgcga aaaatgaaga
     2881 gttaaacaga ttttacttgc agcatacctt attgccaatc gtcaaacagt atgaagaaga
     2941 atttaatcgg aaactactta ctaaaacaga cagagaaaaa aataggtatt ttaaatttaa
     3001 cgttaaatct tatttaaggg ctgatagtgc aacacaagca gaagtgtact ttaaagcagt
     3061 tcgtagtggt tactacacta taaatgacat tagagagtgg gaagatttac caccagttga
     3121 aggtggagat aagccgctaa taagcggtga tttataccca attgacacgc cacttgaatt
     3181 aagaaaatct ttgaaaggtg gtgataaaaa tgtcaatgaa agctaagtat tttcaaatga
     3241 aaagaaaatc aaaaagtaaa ggtgaaatat ttatttatgg tgatattgta agtgataaat
     3301 ggtttgaaag tgatgtaact gctacagatt tcaaaaataa actagatgaa ctaggagaca
     3361 tcagtgaaat agatgttcat ataaattcat ctggaggcag tgtatttgaa gggcatgcaa
     3421 tatacaatat gctaaaaatg catcctgcaa aaattaatat ctatgtcgat gccttagcgg
     3481 catcaattgc tagtgttatc gctatgagtg gtgacactat ttttatgcac aaaaatagtt
     3541 ttttaatgat tcataattca tgggttatga ctgtaggtaa tgcagaagaa ttaagaaaga
     3601 cagcggattt acttgaaaaa acagatgctg ttagtaattc agcttattta gataaagcaa
     3661 aagatttaga tcaagaacac ttaaaacaga tgttagatgc agaaacttgg cttactgcag
     3721 aagaagcctt gtctttcggc ttgatagatg aaattttagg agctaatgaa atagctgcta
     3781 gtatctctaa agagcaatat aagcgtttcg agaacgtccc agaagattta aagaaagatg
     3841 tagacaaaat cactaaaatt gatgatgtag atacatctga attggttgaa acacctaaag
     3901 aaagtatgtc actagaagaa aaagaaaaaa gagaaaaaat taaacgcgaa tgcgaaattt
     3961 taaaaatgac aatgaattat taggaggaaa tgaaatgccg acattatatg aattaaaaca
     4021 atccttaggt atgattggac aacaattaaa aaataaaaat gatgaattga gtcagaaagc
     4081 aacagatcca aatattgata tggaagacat caaacaacta gaaacagaaa aagcaggttt
     4141 acaacaaaga tttaacattg ttgaaagaca agtgcaagac attgaagaga aagaaaaagc
     4201 gaaagttaaa gataaaggag aagcttatca atctttaagt gataatgaga agatggttaa
     4261 agctaaggca gagttttatc gtcacgcgat tttaccaaat gaatttgaaa aaccttcaat
     4321 ggaggcacaa cgtttattac acgctttacc aacaggaaat gattcaggtg gagataagct
     4381 cttaccaaaa acactttcta aagaaattgt ttcagaacca tttgctaaaa accaattacg
     4441 tgaaaaagct cgtctaacta acattaaagg tttagagatt ccaagagttt catacacttt
     4501 agacgatgat gatttcatta cagacgtaga aacagcaaaa gaattaaaag caaaaggtga
     4561 tacagtcaag ttcactacta ataaattcaa agtatttgct gcaatttcag atactgtaat
     4621 tcatggatca gatgtagatt tagtaaactg ggttgaaaac gcactacaat caggattagc
     4681 agctaaagag cgtaaagatg ccttagcagt aagtcctaaa tctggattag aacacatgtc
     4741 attttataat ggatctgtta aagaagttga gggagcagac atgtatgatg ctattattaa
     4801 cgctttagca gatttacatg aagattatcg tgataacgca acaatttata tgcgatatgc
     4861 agattatgtc aaaattatta gtgttctttc aaatggaaca acaaatttct ttgacacacc
     4921 agcagaaaaa gtatttggca aaccagtagt atttacagat gcagcagtta aacctattgt
     4981 gggagatttc aattattttg gaattaacta tgatggaaca acttatgaca ctgataaaga
     5041 tgttaaaaaa ggcgaatatt tgtttgtatt aacagcatgg tatgatcagc aacgtacatt
     5101 agacagtgca ttcagaattg caaaagcaaa agaaaataca ggtccattac ccagctaagc
     5161 cccaaaaggt taatgtaaca gctaaggcta aatcagctgt aatatcagcc gaataggggt
     5221 gatgaaatga gtttagaaga aattaaattg tggttgagaa ttgactataa tttcgaaaat
     5281 gatttaattg aaggtctcat tcaatcggct aagtctgaat tactattaag tggggttcca
     5341 gattatgaca aagatgactt ggaatacccg cttttttgta cagcgattaa atatatcatt
     5401 gcaagagatt atgaaagtcg tggatactca aatgaccaat ctagaagcaa ggtgtttaat
     5461 gaaaaaggat tgcaaaaaat gattttgaaa ttaaaaaagt ggtaggtgat ttttaaatgg
     5521 aatttaatga atttaaagat cgcgcgtatt tttttcaata tataaacaaa ggaccatatc
     5581 cagatgaaga ggaaaaaatg aaattgtata gttgcttttg taaaatttat aatccttcta
     5641 tgaaagatag agaaatttta aaagcgactg aatcaaaatc aggattaacc ataattgtca
     5701 ggtcttctaa aactgaatat ctaccacaaa caaatcactt agttaaaatt gacagtgcat
     5761 tatattccga taaattattc aacattgtag aaataagaat tgatacacca gatattggct
     5821 ataatacagt ggttttatca gaaaaatgag tgtagaaatt aaagggatac ctgaagtgtt
     5881 gaataaatta gaatcggtat acggtaaaca agcaatgcag gctaagagtg ataaagcttt
     5941 aaatgaagca tctgaatttt ttataaaggc tttaaagaaa gagttcgaga gctttaaaga
     6001 tacgggtgcc agtatagaag aaatgactaa atctaagcct tatacaaaag ttggcagtca
     6061 agaaagggct gttttaattg aatgggtagg ccctatgaat cgcaaaaaca ttattcactt
     6121 gaatgaacat ggttatacaa gagatggaaa aaaatataca ccaagaggtt ttggagttat
     6181 tgcaaaaaca ttagctgcta gcgaacgtaa gtatagagaa attataaaaa aggagttggc
     6241 cagataaatg aatatattaa acaccataaa aggaatttta ttatctgatg cagagctcaa
     6301 aacacatata aattctagaa tatactatta caaagtcact gaaaacgctg aaacttccaa
     6361 accctttgtt gttattacac ctgtttatga tttgccttca gactttatgt ctgataaata
     6421 tctcagtgaa gaatacttaa ttcaaataga tgtagaatct tcaaataatc agaaaacaat
     6481 tgatataaca aaacgaataa gatacctgtt atatcaacaa aatttaattc aagcatctag
     6541 tcagttagat gcttattttg aagaaactaa acgttatgtg atgtcgagac gatatcaagg
     6601 catacccaaa aatatatatt ataaaaatca gcgcatcgaa taggtgtgct ttttaatttt
     6661 taaggaggaa ataagcaatg gcagaaggac aaggttctta taaagtaggt tttaaaagat
     6721 tatacgttgg agtttttaac ccagaagcaa caaaagtagt taaacgcatg acatgggaag
     6781 atgaaaaagg tggtacagtt gacctaaata tcacaggttt agcaccagat ttagtagata
     6841 tgtttgcatc taacaaacgt gtatggatga aaaaacaagg tactaatgaa gttaagtctg
     6901 acatgagtat tttcaatatt ccaagtgatg atttaaacac agttattgga cgtactaaag
     6961 ataaaaatgg tacatcttgg gtaggagaga atacaagagc accgtatgta acagtaattg
     7021 gcgaatcgga agatggttta acaggtcagc cggtatatgt agccttactt aaaggtactt
     7081 ttagtttaga ttcaattgaa tttaaaacac gaggtgaaaa agcagaagcc ccagaaccta
     7141 caaaattaac aggtgactgg atgaatagaa aagttgatgt tgatggaacg tcacaaggta
     7201 ttgtatacgg ttatcatgaa ggtaaagaag gagaagcaga attcttcaaa aaagtattcg
     7261 ttggatacac ggacagtgaa gatcattcag aggattctgc aggttcgtta cccagctaat
     7321 ccccaaaatg ttgaagtagc agttaattca aaatctgcaa cagtttcagc agaatagggg
     7381 ctttcaaaat aaatcaaagg agaataattt atgactaaaa ctttaaaggt ttataaagga
     7441 gacgacgtcg tagcttctga acaaggtgaa ggcaaagtgt cagtaacttt atctaattta
     7501 gaagcggata caacttatcc aaaaggtact taccaagtgg catgggaaga aaatggtaaa
     7561 gaatctagta aagttgatgt acctcaattc aaaaccaatc caattctagt ctcaggcgta
     7621 tcatttacac cagaaactaa atcaattatg gtaaataccg atgacaatgt tgagccaaac
     7681 attgcaccaa gcacagcaac gaataaaata ttgaaatata caagtgaaca tccagaattt
     7741 gttactgtag atgaaaatac aggagcaatt cacggtgtag ctgaaggtac ttcagtaatc
     7801 actgctatgt ctactgatgg aagcgataag tcaggacaaa tttcagtgac agtaacaaac
     7861 ggatagggat ttaaggcgca gtatatctgc gtctttttta tttgaataaa aggagctaat
     7921 acaatgatta aatttgaaat taaagatcgt aaaacaggaa aaacagagag ctatacaaaa
     7981 gaagatgtaa caatgggcga agcagaaaaa tgctatgagt atttagaatt agtaaatcaa
     8041 gagaataaaa aagaagcacc taacgcaaca aaaatgagac aaaaagagcg acagttatta
     8101 gtagatttat ttaaagatga aggattgact gaagaagatg ttctgaacaa gatgagtact
     8161 aaaacttata caaaagcctt acaagatata tttcgagaaa tcaatggtga agatgaagaa
     8221 gattcagaaa ctgaaccaga agagatggga aagacagaag aacaatctca ataaaagaca
     8281 ttttatcgaa cattaagaaa atacaacgtt tctgtatgga gcagtatggg tggacattaa
     8341 ctgaagtcag aaaacagccg tatgtaaaac ttttagaaat acttaatgaa gagaataaag
     8401 aagagactga agaaaaacaa agtgaacaaa aagtcattac aggtacggat ttaagaaaac
     8461 tttttggaag ctagaaagga ggttaatatg aatgaaaaag tagaaggcat gaccttggag
     8521 ctgaaattag accatttagg tgtccaagaa tgcatgaaag gtttaaagcg acaattaggt
     8581 gttgttaata gtgaaatgaa agctaatctg tcagcatttg ataagtctga aaaatcaatg
     8641 gaaaaatatc aggcgagaat taaggggtta aatgataggc ttaaagttca aaaaaagatg
     8701 tattctcaag tagaagatga gcttaaacaa gttaacgcta attaccaaaa agctaaatcc
     8761 agtgtaaaag atgttgagaa agcatattta aagttagtag aagccaataa aaaagaaaaa
     8821 ttagctcttg ataaatctaa agaagcctta aaatcatcga atacagaact taaaaaagct
     8881 gaaaatcaat ataaacgtac aaatcaacgt aaacaagatg cgtatcaaaa acttaaacag
     8941 ttgagagatg cagaacaaaa gcttaagaat agtaaccaag ctactactgc acaactaaaa
     9001 agagcaagtg acgcagtaca gaagcagtcc gctaagcata aagcacttgt tgaacaatat
     9061 aaacaagaag gcaatcaagt tcaaaaacta aaagtgcaaa atgacaatct ttcaaaatca
     9121 aatgataaaa ttgaaagttc ttacgctaaa actaatacta aattaaagca aacagaaaaa
     9181 gaatttaatg atttaaacaa tactattaag aatcatagcg ctaatgtcgc aaaagctgaa
     9241 acagctgtta ataaagaaaa agctgcttta aataatttgg agcgttcaat agataaagct
     9301 tcatccgaaa tgaagacttt taacaaagaa caaatgatag ctcaaagtca tttcggtaaa
     9361 cttgcaagtc aagcggatgt catgtcaaag aaatttagtt ctattggaga caaaatgact
     9421 tccctgggac gtacaatgac gatgggcgta tctacaccaa ttactttagg gttaggtgca
     9481 gcattaaaaa caagtgcaga ctttgaaggc caaatgtctc gagttggagc gattgcgcaa
     9541 gcaagcagta aagacttgaa aagcatgtct aatcaagcag ttgacttagg agctaaaacc
     9601 agtaaaagtg ctaacgaagt tgctaaaggt atggaagaat tggcagcttt aggctttaat
     9661 gccaaacaaa caatggaggc tatgccaggt gttatcagtg cagcagaagc aagtggtgca
     9721 gaaatggcta caactgcaac tgtaatggct tcagcgatta actctttcgg tttaaaagca
     9781 tctgatgcaa atcatgttgc tgatttactt gcgagatcag caaatgatag tgctgcagat
     9841 attcagtaca tgggagatgc attgaagtat gctggtactc ctgcaaaagc attaggagtt
     9901 tcaatagagg acacttccgc agcaattgaa gttttatcta actcaggttt agagggttct
     9961 caagcaggta ctgccctaag agcttcattt atcaggctag ctaatccaag taaaaataca
    10021 gctaaggaaa tgaaaaaatt aggtattcat ttgtctgatg ctaaaggtca atttgttggc
    10081 atgggtgaat tgattagaca gttccaagat aatatgaaag gcatgacgag agaacaaaaa
    10141 ctagctacag tggctacaat agttggtact gaagcagcaa gtggattttt agccttgatt
    10201 gaagcgggac cagataaaat taatagctat agtaaatcct taaagaattc caatggcgaa
    10261 agtaaaaaag cagcagattt gatgaaagat aatctcaaag gcgctctgga acaattaggt
    10321 ggcgcttttg aatcattagc aatcgaagtc ggtaaagatt taacgcctat gattagagca
    10381 ggagcggaag gtttaacaaa attagttgat ggatttacac atctccctgg ttgggttaga
    10441 aaagcttcag taggattagc actttttggt gcagcaattg gacctgcagt tcttgctgga
    10501 gggttattaa tacgtacagt tggaagtgct gctaaaggat atgcgtcatt aaatagacgt
    10561 attgctgaaa atacaatcct ttcaaatact aattcaaaag caatgaaatc tttaggtctt
    10621 caaacattat ttcttggttc tacaacagga aaaacgtcaa aaggctttaa agggttagcc
    10681 ggagctatga tgtttaattt aaaacctata aatgttttga aaaattctgc aaagctagca
    10741 attttaccgt tcaaactttt gaaaaacggt ttaggattag ctgcaaaatc tttatttgca
    10801 gtaagtggag gcgcaagatt tgcgggtgta gccttaaggt ttttaacagg acctataggt
    10861 gctacaataa ctgctattac aattgcgtat aaagttttta aaaccgcata tgatcgtgtg
    10921 gaatggttca gaaacggtat taacggttta ggagaaacta taaagttttt tggtggtaaa
    10981 attattggcg gcgctgttag aaagctagga gagtttaaaa actatcttgg aagtatcggc
    11041 aaaagcttca aagaaaagtt ttcaaaagat atgaaagatg gttataaatc attaagcgac
    11101 gatgaccttc tcaaagtagg agtcaacaag tttaaaggat ttatgcaaac catgggcaca
    11161 gcttctaaaa aagcgtctga tactgtaaaa gtgttaggga aaggtgtttc aaaagaaaca
    11221 gaaaaagctt tagaaaaata tgtgcattat tctgaagaaa atagcagaat catggaaaaa
    11281 gtacgtttaa actcgggtca gatatcagaa gacaaagcaa aaaaactttt gaaaattgaa
    11341 acggatttat ctaataacct tatagctgaa atagaaaaaa gaaataaaaa ggaactcgaa
    11401 aaaactcaag aacttattga taagtatagt gcattcgatg aacaagaaaa gcaaaacatt
    11461 ttaactcgaa ctaaagaaaa aaatgacttg cgaattaaaa aagagcaaga actcaatcag
    11521 aaaatcaaag aattgaaaga aaaagctttg agtgatggtc agatttcaga aaatgaaaga
    11581 aaagaaattg aaaagcttga aaatcaaaga cgtgatatca ctgttaaaga attgagtaag
    11641 actgaaaaag agcaagagcg tattttagta agaatgcaaa gaaacagaaa tgcttattca
    11701 atagacgaag cgagcaaagc aattaaagaa gcagaaaaag caagaaaagc aagaaaaaaa
    11761 gaagtggaca agcaatatga agatgatgtc attgctataa aaaataacgt caacctttct
    11821 aagtctgaaa aagataaatt gttagctatt gctgatcaaa gacataaaga tgaagtaaga
    11881 aaagcaaaat ctaaaaaaga tgctgtagta gatgttgtta aaaagcaaaa taaagatatt
    11941 gataaagaaa tggatttatc cagtggacgt gtatataaaa atactgaaaa gtggtggaat
    12001 ggccttaaaa gttggtggtc taactttaga gaagaccaaa agaaaaaaag cgataaatac
    12061 gctaaagaac aagaagaaac agctcgtaga aacagagaaa atataaagaa atggtttgga
    12121 aatgcttggg acggcgtaaa aagtaaaact ggcgaagcct ttagtaaaat gggcagaaat
    12181 gctaatcatt ttggcggcga aatgaaaaaa atgtggagcg gaatcaaagg gattccaagc
    12241 aaattaagtt caggttggag ctcagccaaa agttctgtag gataccacac taaggctata
    12301 gctaatagta ctggtaaatg gtttggaaaa gcttggcaat ctgttaaatc gacaacagga
    12361 agtatttaca atcaaactaa gcaaaagtat tcagatgctt cagataaagc ttgggcgcat
    12421 tcaaaatcta tttggagagg cacatcaaaa tggtttagca atgcatataa aagtgcaaag
    12481 ggctggctaa cggatatggc taataaatct cgcgcgaaat gggataatat ttctagtaca
    12541 gcttggtcga atgcaaaatc cgtttggaaa ggaacatcga aatggtttag taactcatac
    12601 aaatctttaa aagattggac tggggatatg tattcaagag cccacgatcg ttttgatgca
    12661 atttcaagtt cggcatggtc taacgctaaa tcagtattta atggttttag aaaatggcta
    12721 tccaaaacat atgattggat tagagatatt ggtaaagaca tgggaagagc tgcggctgat
    12781 ttaggtaaaa atgttgctaa taaagctatt ggcggtttga atagcatgat tggcggtatt
    12841 aataaaatat ctaaagccat tactgataaa aatctcatca agccaatacc tacattgtct
    12901 actggtactt tagcaggaaa gggtgtagct accgataatt cgggagcatt aacgcaaccg
    12961 acatttgctg tattaaatga tagaggttct ggaaacgccc caggtggtgg agttcaagaa
    13021 gtaattcaca gggctgacgg aacattccat gcaccccaag gacgagatgt ggttgttcca
    13081 ctaggagttg gggatagcgt aataaatgct aatgacactc tgaagttaca gcgtatgggt
    13141 gttttaccaa agtttcatgg aggtacgaaa aagaaagatt ggctagacca acttaaaggt
    13201 aatataggta aaaaagcagg agaatttgga gctacagcta aaaacacagc gcataatatc
    13261 aaaaaaggtg cagaagaaat ggttgaagca gcaggcgata aaatcaaaga tggtgcatct
    13321 tggttaggcg ataaaatcgg cgatgtgtgg gattacgtac aacatccagg gaaactagta
    13381 aataaagtaa tgtcaggttt aaatattaat tttggaggcg gagctaacgc tacagtaaaa
    13441 atagctaaag gcgcatactc attgctcaaa aagaaattag tagacaaagt aaaatcgtgg
    13501 tttgaagatt tcggtggtgg aggcgatgga agctatctat ttgaatatcc aatctggcaa
    13561 agatttggac gctacacagg tggacttaac tttaatggcg gtcgtcacta tggtatagac
    13621 tttggtatgc cttctggaac aaacgtttat gccgttaaag gtggtatagc agataaggta
    13681 tggactgatt acggtggcgg taattctata caaattaaga ctggtgctaa tgaatggaac
    13741 tggtatatgc atttatctaa gcaattagca agacaaggcc aacgtattaa agctggtcaa
    13801 ctgataggga aatcaggtgc tacaggtaat ttcgttagag gagcacactt acatttccaa
    13861 ttgatgcaag ggtcacatcc agggaatgat acagctaaag atccagaaaa atggttgaag
    13921 tcacttaaag gtagtggcgt tcgaagtggt tcaggtgtta ataaggctgc atctgcttgg
    13981 gcaggcgata tacgtcgtgc agcaaaacga atgggtgtta atgttacttc gggtgatgta
    14041 ggaaatatta ttagcttgat tcaacacgaa tcaggaggaa atgcaggtat aactcaatct
    14101 agtgcgctta gagacatcaa cgttttacag ggcaatccag caaaaggatt gcttcaatat
    14161 atcccacaaa catttagaca ttatgctgtt agaggtcaca acaatatata tagtggttac
    14221 gatcagttat tagcgttctt taacaacaga tattggcgct cacagtttaa cccaagaggt
    14281 ggttggtctc caagtggtcc aagaagatat gcgaatggtg gtttgattac aaagcatcaa
    14341 cttgctgaag tgggtgaagg agataaacag gagatggtta tccctttaac tagacgtaaa
    14401 cgagcaattc aattaactga acaggttatg cgcatcatcg gtatggatgg caagccaaat
    14461 aacatcactg taaataatga tacttcaaca gttgaaaaat tgttgaaaca aattgttatg
    14521 ttaagtgata aaggaaataa attaacagat gcattgattc aaactgtttc ttctcaggat
    14581 aataacttag gttctaatga tgcaattaga ggtttagaaa aaatattgtc aaaacaaagt
    14641 gggcatagag caaatgcaaa taattatatg ggaggtttga ctaattaatg caatcttttg
    14701 taaaaatcat agatggttac aaggaagaag taataacaga ttttaatcag cttatatttt
    14761 tagatgcaag ggctgaaagt ccaaacacca atgataacag tgtaactatt aacggagtag
    14821 atggtatttt accgggcgca attagttttg cgcctttttc attagtatta aggtttggct
    14881 atgatggtat agatgttata gatttaaatt tatttgagca ttggtttaga tctgtgttta
    14941 atcgcagaca tccttattat gttattactt ctcaaatgcc tggtgttaaa tatgcagtga
    15001 atacagctaa tgttacatct aatttaaaag atggttcttc aactgaaatt gaagtaagtt
    15061 taaatgttta taaagggtat tctgaatcag ttaattggac cgatagcgag ttcttattcg
    15121 actctaattg gatgtttgaa aatggaattc ctcttgattt cacacctaaa tatactcata
    15181 catcaaatca atttactatt tggaacggtt ctactgatac gataaatcca cgattcaagc
    15241 acgatttgaa aatattaatt aatttaaatg cgagtggagg atttgaactg gttaactata
    15301 caacaggtga tatttttaag tacaacaaaa gtatagataa aaacactgat tttgttttag
    15361 atggtgtgta tgcatatcga gatataaata gagtgggaat tgatacaaat agaggcatta
    15421 taacattagc gccaggtaaa aatgaattta agattaaagg agacgtcagt gatattaaaa
    15481 ctacatttaa gtttcctttt atttataggt aggtgattta atggattatc atgatcattt
    15541 atcagtaatg gattttaatg aattgatttg tgaaaattta ctagatgtag attatggttc
    15601 ttttaaagaa tattatgaac tgaatgaagc taggtacatc acctttacag tttatagaac
    15661 tactcataat agttttgttt ttgatttatt gatttgtgaa aacttcataa tttatcatgg
    15721 tgaaaaatat acaattaagc agacagcgcc aaaggttgaa ggtgataaag tttttattga
    15781 agttacggca tatcacataa tgtatgaatt tcaaaatcac tcagtggaat caaataagct
    15841 tgatgacgac agtagcgaaa ctggtaaaac gccagaatac tctttagatg agtacttaag
    15901 atatggattt gcaaatcaaa aaacttcggt caaaatgacc tataaaataa ttggagattt
    15961 taagcgaaaa gtaccgattg acgaattagg taacaaaaac ggcttagaat actgtaaaga
    16021 agcggtagac ctgtttggct gtataattta cccaaatgat acagagattg gtttttattc
    16081 tcctgaaaca ttttatcaaa gaagcgagaa agtgattcga tatcaatata atactgatac
    16141 tgtatctgca actgtcagta cattggaatt aagaacagct ataaaagttt ttggaaaaaa
    16201 gtatacagct gaggaaaaga aaaattataa tcctattaga acaactgaca ttaaatattc
    16261 aaatggtttt ataaaagaag gtacttatcg taccgaaaca attgggtcta aagctactat
    16321 taactttgat tgcaagtatg gtaatgaaac agttagattt acaataaaaa agggctctca
    16381 aggtggaata tataagttga ttttagacgg caagcaaatt aagcaaattt cttgttttgc
    16441 taagtcggtt cagtctgaaa caatagattt aataaaaaat attgataaag gcaagcacgt
    16501 tttagaaatg atatttttag gagaagaccc caaaaataga attgatatat cttcaaataa
    16561 aaaagctaag ccttgtatgt atgttggaac tgaaaaatca acagtcttaa atttaattgc
    16621 tgataattca ggtcgcaatc aatacaaagc aattgtcgac tatgtcgcag atagtgcaaa
    16681 gcagtttggg attcgatatg ctaatacgca aacaaatgaa gatatcgaaa cacaggataa
    16741 gctgttagaa tttgcaaaaa agcaaataaa tgatactcct aagactgaat tagatgttaa
    16801 ttatataggt tatgaaaaaa tagagccaag agatagcgta ttttttgttc atgaattaat
    16861 gggatataac actgaattaa aggttgttaa acttgatagg tcacatccat ttgtaaacgc
    16921 aatagatgaa gtgtctttca gcaatgaaat aaaagatatg gtacaaattc aacaagcact
    16981 taacagacga gttattgcac aagataatag atataactat caagcaaatc gtataaatca
    17041 tttatacact agtactttga attctccttt cgagacaatg gatataggga gtgtattaat
    17101 ataatggcaa cagaagaagt taaaatcaaa gcgctacttg aaaacgataa acagtacttt
    17161 ccagctacac actggaaagc tataaatggg ataccttatg caggcagtag tgatattgat
    17221 ggattgcctc aagacggtat catttcggta gatgataaaa ataaattaga taaattaaaa
    17281 ataggcgaag caggaattat tcaaaatagc attgtacaga aatccccaaa cggtaaattg
    17341 tggaaaataa cagttgacga tagtgggaaa cttggtacag tgctatttta ttagaaagga
    17401 aggtgcatta tggaaaattt gtatttaata aaggatttgg gagctttagc aggtcgagat
    17461 tatagagcta aagaaattca aaacctgcaa agaatagagc aatttgcgct tggcttgaca
    17521 acagagttta agttgcatca gaaagctaaa acaatgcaac acttcgctga gcaaatttat
    17581 tataatggta gatcgcaagc agcagtaaac aaatctttac aaagtcaaat taacgcactt
    17641 gttgtggcac cacgtaataa cagtgctaat gagattgttc aagctcgagt taatgtaaac
    17701 ggcgaaacct ttgacacatt aaaagaacat ttagacgatt gggaaaccaa aactcaaatt
    17761 aataaagagg aaactataag agaattaaat aagaccaaac aagaaattct tgatatcgag
    17821 tatcgttttg aacctgataa gcaagagttt ttatttgtga cagaacttgc acctcttaca
    17881 aatgcagtaa tgcaatcctt ctggtttgat aatagaacag gcatagtata catgacacaa
    17941 gctagaaata atggctatat gctaagtcgt ttaagaccta atggtcaatt tatagacagc
    18001 tcattgattg taggtggggg tcatggtaca cataacggtt atagatatat tgatgatgag
    18061 ttatggattt atagttttat cttaaatggt aataatgaga atacattagt tcgtttcaag
    18121 tatacgccta atgtggaaat tagctatggc aagtatggta tgcaagatgt atttacagga
    18181 cacccagaaa aaccctacat cacccctgtc ataaatgaaa aagaaaataa aattctatac
    18241 agaattgaga gacctagaag tcagtgggaa cttgaaaact caatgaatta tatagagata
    18301 agaagtttag acgatgttga taaaaatatt gataaagttt tgcataaaat cagtatccct
    18361 atgagactaa caaacgaaac ccaaccaatg cagggtgtga cttttgatga aaaatacttg
    18421 tattggtata caggagacag taatccaaat aatagaaact atttaacggc tttcgattta
    18481 gaaacaggag aagaagcgta tcaggttaat gctgactatg gtggaacact agattcattt
    18541 cctggcgaat ttgcggaagc agaaggtttg caaatatact atgacaaaga tagtggtaaa
    18601 aaagctttga tgctaggtgt tactgtcggt ggtgatggaa atagaacaca tcgtattttc
    18661 atgattgggc aaagaggtat tttagaaata cttcactcaa gaggcgttcc ttttatcatg
    18721 agtgacacag gtggtagagt taaaccttta ccaatgaggc ctgataaact taagaatctt
    18781 gggatgttaa cagagccagg tctttactat ttatacactg atcatacagt tcaaatcgat
    18841 gatttcccat taccaagaga atggcgtgat gcaggttggt tcttggaagt taagccacca
    18901 caaactggcg gtgatgtaat tcagatattg acgcgtaata gttatgcaag gaatatgatg
    18961 acttttgaaa gggtgctttc tggaagaact ggagacattt cggactggaa ttatgtgcct
    19021 aaaaatagtg gtaaatggga gagagtacct tcattcatca caaaaatgtc agatattaac
    19081 atagtaggca tgtcgtttta tttaactacg gatgatacaa aacgttttac agattttcca
    19141 actgaacgta aaggggtagc tggttggaac ttatatgtag aagcttcaaa cacaggtggc
    19201 tttgttcata ggctagttcg taatagtgtt acagcatctg ctgagatact attgaaaaat
    19261 tatgatagta aaacaagttc agggccatgg actttacacg aagggagaat tataagttaa
    19321 tgagtaattt agagaaatct gtagctataa atttagaaaa cacagcgcat tatgaaaata
    19381 tttcaaatct agatataact tttagaacag gagagagtga ttcttctgtt cttcttttta
    19441 atatcattaa aaataatcaa ccgttattac tgagtgaaga aaatatcaaa gcacgaatag
    19501 cgattcgagg taaaggagta atggtagttg ctccactaga aatattagat ccatttaaag
    19561 gtattttaaa atttcaatta cctaatgatg taattaaaag agatggaagt tatcaagctc
    19621 aagtttcggt tgcagaatta ggtaattcag acgtggtagt tgtagagaga actatcacat
    19681 ttaacgttga aaaaagtttg tttagcaaga ttccctctga aacaaaacta cactatattg
    19741 ttgagtttca agaattagaa aaaactatta tggatcgcgc gaaagcaatg gacgaggcta
    19801 taaaaaatgg tgaggattat gcgagtctga ttgaaaaagc taaagaaaaa ggtctatcag
    19861 atattcaaat agcaaaatct tcaagtattg atgaattaaa gcaacttgct aatagccgta
    19921 tatctgattt ggaaaataaa gcgcaagcat attcaagaac attcgatgag caaaagcgat
    19981 atatggatga gaaacatgaa gccttcaagc agtcagtgaa tagtggtggt ttagtcacaa
    20041 gtggttctac ttcaaattgg caaaaagcta agattactaa agatgatggt aagataatgc
    20101 agattactgg atttgatttt aataatccag aacaaagaat aggtgattca acccaattta
    20161 tttatgtttc gcaagctata aattatccaa gagatgttag tactaacggt actgtcgaat
    20221 atttagtagt aacttcagat tacaagcgta tgacttatcg accgaacggt acaaataaag
    20281 tgtttgttaa aagaaaagaa gcgggttcat ggtctgagtg gtcagaatta gctattaatg
    20341 attacaatac accttttgaa actgttcaaa gtgcccaatc aaaagctaat atggccgaaa
    20401 gtaacgctaa attatacgca gatgacaagt ttaataaaag gtattcggtt atttttgatg
    20461 gaacagcaaa tggtgtgggc tctacattgt acttaaatga gagtttagac caatttattt
    20521 tattaatttt ttatgggact tttccaggtg gtgactttac agagtttggc agtccttttg
    20581 gaggaggaaa gatttcattg aatccctcaa atcttccaga tggtgatgga aatggtggag
    20641 gtgtttatga gtttggatta actaaatcta gtcgtacatc tttaactata tcaaacgatg
    20701 tctatttcga cttaggaagt caaagaggct ctggtgcgaa cgcaaataga gggacaatta
    20761 acaaaattat aggagtgaga aaataatgca aatattagtt aacaagcgta atgagataat
    20821 ttcatacgct atcattggcg gctttgaaga aggtattgat attgaaaatt taccagaaaa
    20881 tttctctcaa gtttttagac ctaaagcctt taaatattca aatggggaaa tagtttttaa
    20941 cgaagattat tcagaagaaa aagatgactt gcatcaacag attgacagtg aagaacaaaa
    21001 cacagtcgct tctgatgaca tcttacgaaa aatggttgct agtatgcaga aacaagttgt
    21061 tcaaagtaca aagttatcga tgcaagttaa taagcaaaat gcactaatgg caaaacaact
    21121 tgtgacactt aataaaaaat tagaagaggt taaaggagag actgaaaatg cttaaattaa
    21181 tttcaccaac attcgaagat attaaaacat ggtatcaatt gaaagaat
//
LOCUS       DAMQYT010000022        20559 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMQYT010000022 DAMQYT010000000
VERSION     DAMQYT010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20559
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.022"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            51..1508
                     /locus_tag="PGA33_000698"
     CDS             51..1508
                     /locus_tag="PGA33_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDG6472531.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            1501..2223
                     /locus_tag="PGA33_000699"
     CDS             1501..2223
                     /locus_tag="PGA33_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDG6472532.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            2374..3501
                     /gene="queG"
                     /locus_tag="PGA33_000700"
     CDS             2374..3501
                     /gene="queG"
                     /locus_tag="PGA33_000700"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDG6472533.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPLIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            3506..3976
                     /gene="trmL"
                     /locus_tag="PGA33_000701"
     CDS             3506..3976
                     /gene="trmL"
                     /locus_tag="PGA33_000701"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDG6472534.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEVTNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            4135..4734
                     /locus_tag="PGA33_000702"
     CDS             4135..4734
                     /locus_tag="PGA33_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HDG6472535.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            4759..4911
                     /locus_tag="PGA33_000703"
     CDS             4759..4911
                     /locus_tag="PGA33_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472536.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
     gene            5688..6083
                     /locus_tag="PGA33_000704"
     CDS             5688..6083
                     /locus_tag="PGA33_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472537.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPVIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITAILYYTLDGFYASLTGVLGTLVAL
                     NPYTTIVSAVLYILTAIATVEATKTKYRN"
     gene            6279..7664
                     /gene="fumC"
                     /locus_tag="PGA33_000705"
     CDS             6279..7664
                     /gene="fumC"
                     /locus_tag="PGA33_000705"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDG6472538.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAAIANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(8127..8948)
                     /locus_tag="PGA33_000706"
     CDS             complement(8127..8948)
                     /locus_tag="PGA33_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDG6472539.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTIDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            9111..10223
                     /locus_tag="PGA33_000707"
     CDS             9111..10223
                     /locus_tag="PGA33_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HDG6472540.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            10245..10868
                     /locus_tag="PGA33_000708"
     CDS             10245..10868
                     /locus_tag="PGA33_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDG6472541.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            11224..11688
                     /locus_tag="PGA33_000709"
     CDS             11224..11688
                     /locus_tag="PGA33_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDG6472542.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            11867..12991
                     /locus_tag="PGA33_000710"
     CDS             11867..12991
                     /locus_tag="PGA33_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDG6472543.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLNQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASKQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            13060..13404
                     /locus_tag="PGA33_000711"
     CDS             13060..13404
                     /locus_tag="PGA33_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HDG6472544.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            13787..13885
                     /locus_tag="PGA33_000712"
     CDS             13787..13885
                     /locus_tag="PGA33_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472545.1"
                     /translation="MPFDSLAVLITTEIAFPTEIFEKYQRTLQIKF"
     gene            14176..15372
                     /locus_tag="PGA33_000713"
     CDS             14176..15372
                     /locus_tag="PGA33_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDG6472546.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            15362..18298
                     /locus_tag="PGA33_000714"
     CDS             15362..18298
                     /locus_tag="PGA33_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDG6472547.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQSNIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            18295..19236
                     /gene="yhaM"
                     /locus_tag="PGA33_000715"
     CDS             18295..19236
                     /gene="yhaM"
                     /locus_tag="PGA33_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HDG6472548.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(19357..20319)
                     /locus_tag="PGA33_000716"
     CDS             complement(19357..20319)
                     /locus_tag="PGA33_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HDG6472549.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
BASE COUNT         7550 a         2876 c         3616 g         6517 t
ORIGIN      
        1 aatgtattaa tatgtataat tatgaatatt aattagggag ggctgtataa atgaagtgtt
       61 taattaggtt tatattagta ttagggttgt taattagcag tgctatggtg tatatcaatc
      121 ccacagcaca tgctgaacaa gatcaaacat gggagaagat taaagaacgc ggtgaactta
      181 gagtgggtct ttctgcagat tatgcaccaa tggaatttga gcatacagtt aatggtaaga
      241 ctgagtatgc aggtgtagat attgatttag ctaaaaaaat tgcgaaagat aataatttaa
      301 aattaaaaat cgtcaatatg tcatttgata gtttgttagg agctcttaaa actggaaaaa
      361 ttgatattat tatttccgga atgacttcaa cgcctgaacg taagaagcaa gttgattttt
      421 cagattcata tatgatgact aaaaatatca tgcttgtaaa gaaagataaa gttaatgaat
      481 ataaagatat caaagacttt aataataaaa aagtaggggc acaaaaggga actgaacaag
      541 aaaaaatcgc tcaaaccgaa attgaaaatg catctattac ttcattaagc cgtttaccag
      601 acgttatatt agcattgaaa agtggaaagg ttgaaggtgc tgtagttgaa aaacctgtag
      661 cagaagcata tttaaaacaa aatcctaaat taggaatttc caatgtgaaa tttaatgaag
      721 aagaaaaaga tacagtgata gcagtgccaa aagattcacc aaaattattg tcacaaatta
      781 ataaaacgat taaggaggtt aaagataaag gattaatcga taaatatatg actaatgctg
      841 caaatgcgat gaatgatgac agtggtttta tttctaagta tggtagtttt ttcttgaaag
      901 gaattaagat tacaatatta atttcactta tcggtgttgc attaggttct attttaggtg
      961 cattcgttgc gttaatgaaa ttaagtaaaa taaaaattat ttcatggatt gcttctatct
     1021 atatcgaaat attaagagga acaccaatgt tagttcaagt atttatcgta ttctttggta
     1081 taactgctgc attaggacta gacatttcag ctcttgtttg tggaacaatt gcattagtta
     1141 ttaattcctc agcttacatt gctgaaatta ttcgtgcagg tataaatgct gttgataaag
     1201 gccaaatgga agccgcacgt agtctaggtt tgaattatag acaaacgatg aaaagtgtaa
     1261 ttatgccaca agcaattaaa aatattttac cagctttagg taatgaattt gtcactttaa
     1321 ttaaagaatc atctattgtg tcaacaattg gagttggcga aattatgttt aatgcacaag
     1381 tggttcaagg tatttcattt gacccattca caccattaat agtggcagca gcattatact
     1441 ttgtcttaac atttgtactt acacgcatta tgaacatgat tgaagggaga ttgaatgcca
     1501 gtgattaaaa taaacaatct taataaagtt tttggagata atgaagtttt aaaagatatc
     1561 aatcttgaaa tcaatcaagg ggaagtagta gcaataatag gtccatctgg tagtggtaaa
     1621 agtacattgt taagatgtat gaatttatta gaagtaccta ctaaaggtca agtgattttt
     1681 gaaggcaatg acttaacgga aaaagggaca caagtagata aactacgtca aaaaatgggt
     1741 atggtatttc aaaacttcaa cctatttcca cataaaaaag ttgtcgataa tattatttta
     1801 gctcctaaat tattaaagaa agataataac gatgaattac ataaggaagc attgtcgtta
     1861 ttagataaag tgggattaaa agaaaaagca gatgtatatc cgaatcaatt atcaggtggt
     1921 caaaaacaaa gggtagcaat tgcaagagct ttagcaatgc atccagatgt tattttattc
     1981 gatgaaccaa cttcagcatt agatcctgag gtagttggtg atgtattaaa agtaatgaaa
     2041 gacctagcca aagaaggtat gaccatggtg gttgtgacac atgaaatggg atttgccaaa
     2101 gatgtaagtg acaaagtcat atttatggca gatggcgttg tcgtagagtc aggcacacca
     2161 gtcgaaatat ttgaacaacc gcaacatgaa agaacacaaa atttcttagc aagagtatta
     2221 taacaaccta acgaagcttg aatatatgat acgcaccaca aagttatatc atatattcaa
     2281 gctttttatt attaaaatta tgtgaaatga aacctcaata tgaataatgg ttgtgttaaa
     2341 ataacatagt attattttat ggggggcata cgattggata caaagcaatt aaagcaagat
     2401 atcattgact atgcatatac aattggtatc gacagtattg gatttactac tgccgatccc
     2461 tttgatgaat tgaagcaaaa gctagaagca tatcatgcaa atggttatgc ctcaggattt
     2521 gaagaatctg atattgcttt acgaacggag cctaaattat ccttaccaac agcaaggtca
     2581 atcatagcaa ttgcagttgg ttatcctaac aaactgaaag gtgcacctaa gagtgttaga
     2641 ggtgatcgca gaggcttatt tgctagagca tcgtggggtc aagattatca tacaattatg
     2701 cgtaaacgat tagacatgtt agctgcattt attgaatcta aagttccaga tgttgaaatc
     2761 aaatctatgg tagatacggg tgtattatca gatagggcag tagcagaacg tgcaggttta
     2821 ggatttgttg gtcgtaatgg ctttgtcatc aatcctaaac taggaacatg gacatacctc
     2881 ggtgaaatgt tagtcagtat accttttgaa cctgatgatc cattattaga tagctgtggt
     2941 gattgtacaa tttgtgttga tcgttgtcca acaagtgcat tagttggaaa tggccaatta
     3001 aatagtcaga aatgcattag ttttttaacg caaacaaaag gctatatgcc tgaccaatat
     3061 cgttataaaa ttgggaatag attatatggt tgcgatacgt gtcaacaagt ttgtccgaaa
     3121 aatagaggta ttaataccga acaagatgac atcattttgg aaccagaaat tttaaagcca
     3181 agattagtac ctttactacg tatgtctaat aaagaattca aacaaacata tggtcacctt
     3241 gcaggtgctt ggcgtggtaa aaagccgata caacgaaatg ctattttagc attggcgcat
     3301 tttaatgaag tggatgcaat tccagaattg aaaaaagttg caacaacaga tgaaagaccg
     3361 ttgattcgag caactgctta ttgggccatt ggtcaaattc ttggggaaga agcaagagat
     3421 tttatcaatg ctaattatga tcaagaggat gcagaagttc agaatgaaat gataaaagga
     3481 ttagacacaa ggagagaata gtaatatgac aaatcatatc gttttatatc aaccagaaat
     3541 cccagctaat actggtaaca ttgcacgtac gtgtgcaggt acaaacacac atttacattt
     3601 aattaaacct cttggattta gaactgatga taaaatgtta aaaagagccg gtttagatta
     3661 ttgggaattt gtgaatatta cgtatcatga tagtattgaa gcattttttg aagtcacaaa
     3721 tggcgagtat tacttattaa caaaatttgg taaaaagact tatagtgatt ttgatttttc
     3781 aaatcatgac aaagattttt acttcatttt tggaaaagaa acgacaggat taccagactg
     3841 ggtgaaagag aaatatcaag acacagcgtt aagaattcct atgagtgaac atattcgttc
     3901 attaaattta tcaaatactg ctgcgttgtt aatttatgaa gcgttacgtc aacaagattt
     3961 tccgggattg aattaaaatt tactcatttt cattataaaa atgatgctta agtgctatat
     4021 ttagagtata agcatgttaa agttaaatgg tttgatatat tgaaagttag ggtataataa
     4081 ttattaatat cgccgttttt atgtaaaatg caaactaaga ggagtgtaga aatcatggca
     4141 atgaacttta aagtctttga caatagtcaa cttgtagcag aatatgctgc tgatattatt
     4201 agaaagcaat ttaacaataa tcctactaca attgcaggtt ttcatttaga tacagatcaa
     4261 gcgccagttc tagatgaatt aaagaaaaat gttgaaaaac atgctgttga ttttagccaa
     4321 ataaatattt tagattatga cgataaaaaa tcatatttcg aagcgttagg tgtaccagca
     4381 ggtcaagttt atccaattgc ttatgaaaaa gatgcaatcg aattaatcgc tgataagatt
     4441 aaaactaaag aaaataaagg gaaattaaca ttacaagttg tttctatcga tgagcaaggt
     4501 aagttaaatg ttagtattcg tcaaggacta atggaagcaa gagaaatttt cttagtagtg
     4561 acaggtgcta ataaacgaga tgtagttgaa aaattatatc aagaaaatgg taaaacaagc
     4621 ttcgaaccag ccgatttaaa agcacataga atggtaaatg ttattcttga taaagaagcg
     4681 gctgcaggtt tacctgaaga tgttaaagct tactttacgt cacgctttgc ttaattaaaa
     4741 atctgtgagg tattgattat gtctaaagac aaagatccaa aattaaatta tcatgaagaa
     4801 gaaaacagta tggtaacgga ttttgaagat ttaaaagaat taggtaaaga aatggaacaa
     4861 atctctgatc aaaatgatca agaaaagaat tctgaagaag acagtcagta aagtattata
     4921 attgtgtaat attcagttat ttatcatgta aataatgtat agtatagaac atatatcaaa
     4981 taggattgat tatcttgaat atgaagttag gtagcatttt gttacctagc ttttttatta
     5041 tattcataga agctgacttt ctgctagttt cagtgttggg gctgtttccc caacttacat
     5101 tgtttgtaga aattgaggat ccaatttttc tatgttgtgg ccccgccaac ttgcatgtta
     5161 ttttaagctg actttccacc aacttctgag tttgtggccc gtttccctaa tttgcaatgt
     5221 ctgtagaaat tagaaatccg atctcactat tttggggcct cgccaacttg catgttattt
     5281 taagctgact ttccaccagt ttctgagttt gtggcccgtt tccccaattt gcaatgtctg
     5341 tagaaattgg gaatccaatt ccactatgtt ggagctccac caacttgcat gaaatagaag
     5401 ctgacattcc gaagcctcta tgttgtggac tgcagtcaac tactgccaat ataactttgt
     5461 aaagcataaa acattgattt gtgtaccaga catagtaata tatcaaaagt tcaattttaa
     5521 atattaaaac accatttaaa cggaattgat aattaattac tccagacata attgaaagaa
     5581 gtcatctatc agtaatattt ttaatgaaat cattaaaccg cgtttcttaa tatgccaaga
     5641 ttatgatatg ctattgaaga atgaaattag aggagattta tatatttatg ttaggaaaat
     5701 actggattca attattgata gcaacagtta ttataagttt aatatcaatt aaagcttttc
     5761 cattggcaat tggggcatta tatttacctg ttattttcaa agtaataaaa ttacaactca
     5821 atttatcaaa aggattaatt gatgatgtta atgcgcaaac attcattaaa agtaatcaat
     5881 caggtattgt aatcagtgta atttgttgtt tattaataac agcaatattg tattatacac
     5941 ttgatgggtt ttatgcgagt ttaacaggag ttttaggtac gcttgttgct ctaaatccat
     6001 atacgacaat tgttagtgct gtattatata tacttacagc tattgctacg gttgaagcta
     6061 ctaaaacaaa gtatcgtaat taaacttagt tcaataataa ctaaataatg atagataaca
     6121 cactaatcgt atgaccgtat cactgtaata aagggtgaac acgtgcgatt agtgtatttt
     6181 tatttcaaat tatgaaaacg ttttaatcat aaattgctca attaaattat ttaatgatat
     6241 actacagtta aataaacagt gataagggga gaaattgaat gtcagtaaga attgaacatg
     6301 atacttttgg agaaatagaa gtacctgcag ataaatattg gggtgctcaa acagaaagaa
     6361 gtaaacgtaa tttcccagtt ggtaaagagc gtatgccaat cgaagtagtt tatggttttg
     6421 cacaactaaa gcgtgcagca gcaatagcta attttgattt aggaaaatta agcgaggcaa
     6481 agaaagatgc cattgtatac gcatgtgatc aaattttatc aggtgaatta gatgaacact
     6541 tcccactagt tgtatggcaa acaggaagcg gtacacaaag taatatgaat gtgaacgaag
     6601 tagtaagtta tgttgctaat atgtatttaa aagatcatca aagtgatgaa agtatccacc
     6661 caaatgatga tgtaaataaa tctcaaagtt cgaatgatac attcccaact gctatgcacg
     6721 ttgcattata tcaagaggtt gaaacaaaat tagaacctgc attaaaactt ttaagaaata
     6781 ctttgaaaga aaaagaagat aaatttgatt caattattaa aattggtcgt acacatttac
     6841 aagatgcaac gccgatcaaa ctaggacaag agattagtgg ctggcgttat atgcttgacc
     6901 gttgtgaaac aatgttatct gaatctaaga agcacatttt aaatcttgcc atcggtggta
     6961 cggctgttgg tactggtatt aatgcgcatc ctgaatttgg tgataaagtg gcacattata
     7021 tttcagaaaa tacgggttat ccatttgtat cttctgaaaa taaattccac gcacttacag
     7081 cgcatgatga agttgttcaa ttgcatggaa cattgaaggc attagcagga gacttaatga
     7141 aaattgctaa tgatgtgaga tggttggctt cagggccacg agctggtttg gcagaaattt
     7201 ctatccctga aaatgaacca ggttcatcaa ttatgcctgg taaagttaat cctacacaat
     7261 gtgaaatgtt aacaatggtt gcagtccaag taatgggtaa tgatacagtt gttggcttcg
     7321 caagttcaca aggtaacttt gaattgaatg tttataaacc agttattatg cataatacac
     7381 tacaatcaat ttatctttta gctgatggta tggaaacatt taataacaat tgtgcagtgg
     7441 gcattgaacc aatcgaagag aatattgata attatttaaa tcaatcatta atgttagtta
     7501 ctgcattaaa tccacatatt ggttatgaaa aagcagctca aattgctaag aaagcccata
     7561 aagaaggttt aactttaaaa gaatctgcaa ttcaaactgg atatgttaca gaagaacaat
     7621 ttgaagcatg gattaaacca gaagatatgg tagatcctca ttaattaaat attaatataa
     7681 aataaagttt gggcattaca ggtagatgta gtgttcaagc tttatttttt gtctatgtca
     7741 aattgaactt ataagtctga tttgatatag agccatgaaa aggaaaaact tttcatggag
     7801 tccgttttat tatagaacct attttttata tgatgaattg ttgtacatgt attggccaaa
     7861 caacctgtga taaaaatctt atgatgtcat gtattgtcca tagatacacg taacgcagtt
     7921 gaaaaagact acaagtcatt atccaaggat gttataaaat gaataattac taaaatatca
     7981 aaatataaaa tcctaagcat cgtaaaaaac aaaaaaaccg aacatctgaa aaaattaata
     8041 attttccatc tatccgattt aagttgatac ttgatttagt tacaatgcgg tattgcatgt
     8101 ttaaatatca tgcaaaacta atcaaattag tcatccaaag aaacggaaat gacttcctta
     8161 gtcaagggat gcacaaactc tattttgtaa ctttcaagtt ctaattgtct taacgttgaa
     8221 tcaccatata aaggatcacc aataacaggg tgaccaattt cagctaaatg cacacgaatt
     8281 tgatgagtac gtccagtatc caacttgatt tcaagttggc acacagcttc ttttatcatt
     8341 tttgaagtta aaatgtgtgt aattgcacgc tgtccagtag gagacacacg tcgtttattc
     8401 gaatggaatt tatctttacc aattggcata tcaatcgttt gtggttttaa aggtaaaagt
     8461 gccttaacat ttgctttgta tatccgcgta atatcattgt cttctaacat gcgatcaaga
     8521 atttttttca ttaaaggatt tttagcaaca attaataatc ctactgtttc ctggtccagt
     8581 cgatgaattg gttcgacata gtcactatca atagtgtaaa tcacatgatt cattaaagta
     8641 ttgctttctt ttaaatcatt agggtgagtc ttaacacctt taggtttcat tacgattgcc
     8701 atatcatcgt cttcgtattt aatttgtgca taacgataac ttggaacata attacttttt
     8761 tcatcgatgg ttggaacaaa tacatcgtcg ccagtatgca ctttatccat taatcgcgca
     8821 ggtttatcat taatagtaat atctttagac atatttaaat gatgtaaatc ttttttaggt
     8881 accttaagtt gttggaaaat atctcgtaaa cttaagtcat taaagttttc tggtatttta
     8941 aatttcataa aatcctccta tagtctttaa tatcatatca caaatcagtt ctccgaaata
     9001 taactattga gtaatcaagg ccattttatc atgtttacgt ctaagattta aaaatataat
     9061 aactcaatgt ataatagtta atacacgtaa aaatgaattg gagcgatttg atggaacaaa
     9121 ggacgcgact agctttatta aaagagattg ccgaattttt aaacgaagaa actgaaatgt
     9181 atagcatgac ccaaggtgcg ttaaaatatt taattgaggg cagtaatttc acgacaggct
     9241 ggatattttt catcaatagc gtaggtgaac atgaacttgt atcacatgtg gctctaccac
     9301 aatctttgac agcagaccac tgtcactata ttaaagatgg gtcttgttgg tgtgtgaaag
     9361 catttaatca acgtaggcta atgaaagcgt cgaatatcgt caattgttct cgtattaact
     9421 tagcctctaa agcatttcct agccaaaatg acaatattac acatcatgca acggtaccac
     9481 ttaaatcggg tcaagaacaa ttcggcattt taaatgtagc gtctccaaat actgaaatat
     9541 atagcgatga agatttagag ttgctagagt ctgtcgcatt tcaattaggg tcagccatca
     9601 aacgtattta tttaacagac cgtgaaaaag aagcggcaaa aattaatgaa cgtaatcgtt
     9661 tagctagaga tttacatgat tcagtaaatc aaatgttatt ttctgtaaaa ctaacagcac
     9721 atgctgcgta tggcatgtca aacgaatcca tcgctaaaca agcattcaag acgattgagg
     9781 aaacgagtca aaatgccgtt aatgaaatgc gtgcattgat ttggcaactt aagccagttg
     9841 gacttgagca aggtttaatt catgctttga cagcatatag taaattaatg catatacaat
     9901 taaatgtaaa tgtagaaggt ttaatcgatt tatctaatga aatcgaagaa aacatataca
     9961 gagcattaca agagtgtatt aataatgtta agaaacatgc tgatacgaat aaaatggatc
    10021 ttacattaaa acaaatgaac gatatactat atattgacgt gattgattat ggtcaaggat
    10081 ttgagatcga taatgttcag atagcttcat cacacggtat taataacata aaacaaagag
    10141 ttaaattatt aagaggtaaa gtgacctttc attctcagcc aacaaagggt acacaaatac
    10201 aattcacaat tcctataaaa tagcttgatt taaggagata acccatgaac aaagtaatat
    10261 tagtagatga ccattatatt gtgcgacaag gattgcgatt tttattatcc acgattgaaa
    10321 acatagaagt tttacaagac tttgcagatg gagaaacatt tttagaatat ttaaaagagc
    10381 atgagcaccc tgatattgtg ctattagatt tagtgatgcc tggcatgaat ggtattgaaa
    10441 ttacggaata tattaaggca cattatccgg atattaaagt tttggtatta acaagttatg
    10501 ttgatgatga acatgtaatt tcagcaatca ataaaggtgc tgatggttat gaaatgaaag
    10561 acgttgagcc tcagcaatta attgaaacta ttagacgagt tatgaacggt gaaaaaatga
    10621 tacatcctaa ggcacaagat gtattcgaaa cagttagcca aaaaccacac tacacgaata
    10681 agttgtcaaa gagagaaatt gaagtgttac gtgaaatggt taaaggtaaa acaaataaag
    10741 agattgcaga aactttattt gtatctgaaa aaacaattaa aacacatgtc agtcatatat
    10801 ttagtaaatt acaagttagc gatcgtacac aagcagcaat ttatgcaatg gaaaataagt
    10861 tgatttagaa tgagctttta aatatttgtc gaaaaataga caatttttaa tctgattttg
    10921 atgaattttt tatagcgata gggtttatta tacagttttc tttgtaaatt tgatatcatt
    10981 ataaaagtgt aagttttatc aaatctagtg attatgcata aaaatttact tcatttactt
    11041 tcgcttcatt ataaagtaaa cttaactctt gttgacttga tcaaaattta atacaaatgt
    11101 gaaattgact cttttcattt tattaaaaat atattaagat gtaatctgtg tttcaagaaa
    11161 tgtgtattat tgcaatttct tgtgtccgga taaaattccc caaacagaaa ggtagataac
    11221 agaatggata gacagagttt tacagattta attcaaacaa aatttaaaat ggttcgtata
    11281 gaggctggtt atacgcaaga tactatggcg caaacaattg gactttctaa aaagacttta
    11341 gtacaaatcg aaaaagagag agtattacca aactggacaa cttgtatttc aatttgtgca
    11401 ttattcagag actctgaagt attaaacagt acatttggct gtgatccatt agaaatcgtt
    11461 caaacaattt caagaaatca ctgtgcatat ccaaaccatg caccaacaag tgatatttat
    11521 tggaataaca ttgagactcg taatgggtac attttacaaa gtaacaaagt aagcaatatt
    11581 tatcgtgtat taaatccaga taaccaacca attttcggta cttctaaaat gagagaagct
    11641 gaaacatact ttaacagaaa tgctaaagaa gaattagtac atatctaggc ctatatactt
    11701 ttaataaata ataaaagcga tgtaacacac ctgtttcgag aggtgaggca tcgctttttg
    11761 tatgtaaact aataataaat tgagcagaaa gtgaaatgat taatgaatta aaaaataaaa
    11821 atagtggtat aatgaataac tatgttgtca caaaggagtc aaaattatga atgcactatt
    11881 tattatcata tttatgattg tagtaggggc gataattggt ggcattacta atgtaattgc
    11941 aattagaatg ctgtttcacc cctttaaacc atactatata tttaaattta gagtcccatt
    12001 tacaccaggt ttaataccga aaagacgcga agaaattgca actaaaattg gccaagtgat
    12061 tgaagagcat ttgcttacag aaactttaat taatgaaaaa ttgaaaagcg agcaatcaca
    12121 gcaagcaata gaatctatga ttcaacagca gttacaaaag ttgacgaaag atcaattgtc
    12181 aataaaacaa attacttctc aaatcgatat tgatttagaa caagtattac aaactaatgg
    12241 aaatcaatat attgaatcac aattgaataa ttattataca aagcatcaaa accaaacaat
    12301 agcatcttta ttgccaaatc aacttgtaac atttttaaat cagcatgtag ataacgcaac
    12361 agacttatta tgtgatcgtg caaggaatta tttatcatct gcaaaaggca cacaagatat
    12421 taatgatatg ttggatactt ttttcaatga gaaaggtaag ttaattggta tgttgcaaat
    12481 gtttatgaca aaagagagta ttgcagatcg cattcaacaa gaacttatac gtttaacatc
    12541 tcatcctaaa gcaagaacaa ttgtgacatc gttaattacc aatgaatatc aaacttttaa
    12601 agataaacca ttgaatgaac tgttagatgc atcgcagttt aatgaaatcg ctgaaaatct
    12661 atcagtgtat gtaacaacat atgcaagcaa acaagcgaat aagccagtgg ttacgttaat
    12721 gcctcaattc gttgattatt tagaaggtca actctctagc aaattagcaa atttaatcat
    12781 tgaaaaatta tcgattcact tatctacaat tatgaaaaaa gtggatttac gaggattaat
    12841 tgaagaacaa attaatacgt ttgacttaga ttatattgaa aaattaatta tagaaattgc
    12901 taacaaagaa ctaaagttga ttatgtcatt aggtttcatt ttaggtggta ttattggatt
    12961 tttccaaggt ttggttgcaa tctttgtcta aaaaagcgag atgtggtaaa gtaatatcga
    13021 acgttttaat aaacatcaca acaaaggagt gctataacca tggcagtaaa tttatatgat
    13081 tatgcaaatc aattagaaca agctttaaga gaaagcgaag aatacaaagc aatcaaagaa
    13141 gcattcgcta atgtaaaagc taacgaagaa tctaaaaagt tattcgacga gttccgtgaa
    13201 actcaaatta acttccaaca aaaacaaatg caaggtgaag aaattgctga agaagattta
    13261 caaaaagcgc aagaacaagc gcaagcaatt gaaaaagatg aaaacatctc tgcattaatg
    13321 aatgctgaac aaaaaatgag tcaagtattc caagaaatca accaaattat cgttaaacca
    13381 ttagacgaaa tttacgctga ctaatattaa aataatattt ttcaatcctt taaatagagg
    13441 aatagactgc cgacagagtc cgagactttg tgggtagttt ttttagtttt gataacggaa
    13501 gttagaggct ctctgtcaaa ttgggcagat gcattcagta acggaagtta ggctagtaaa
    13561 cgtgtcctaa cttccttttt gcattttgaa gtgtaaagtt ttcaacataa tactattagt
    13621 tcggtcatgt atcggactga tggaaaagcg tttcactttt aatgactcat taagaacggc
    13681 ctgaaaatgt ttggcgtatt aagtgcaatg atagttttga catttagttt ctaattggtc
    13741 attactgccg agcaaatcta gtagagtaat catgtaaatc tttaatgtgc catttgattc
    13801 actagcggtg ttaataacta cggaaattgc atttccgact gaaatttttg aaaaatatca
    13861 acgtacgcta caaataaaat tttaaactgt tataaatgtg tctcaatttc atatgttcat
    13921 cgacgatatg aagcgtatta tggtaaaatg aagaaataat aaacttgtta ataaataaaa
    13981 catcacgatt tgactaaagc actttattat tgtgtagata atagtttttt aacgaaataa
    14041 aaatggcgac tggttttaat aaatcagcta atgaatcact acacctataa gtatgaatat
    14101 agtgattaga atgctttgta tagttggatt ttgcaaaatt gatgttagaa cctttaatta
    14161 aggagatatg gatgtatggt taaatttatt cattgttcgg atttacattt agatagtcct
    14221 ttcaaatcta agagtcatat aagccctaaa atttttgagg atgttcaaaa aagtgcttat
    14281 gaaagtttta aaaatattgt agatattgca ttacaacaag atgttgattt tgtaattatc
    14341 gcgggtgatt tatttgatag tgaaaacaga acattacgtg ctgaaatttt cttaaaacag
    14401 caatttgaac gtttacaaaa tgaacaaatc tttgtttatg tttgtcatgg taatcacgat
    14461 cctttatcat caaagatttc atcaaactgg ccagataatg tttctgtatt ttcaaataaa
    14521 gttgagacgt atgaagcaat tactaaatct ggtgaaacaa tttatattca cggatttagt
    14581 tatgaaaata gagcaagtta tgagaacaag attgatgaat atccatcaag tcaaggccaa
    14641 aaaggcatac atattggtgt cttgcatggt acgtatagta aatcttcagt taacgaaaga
    14701 tataccgagt tcattttaga agatttaaac agtaaattgt atcattattg ggctttaggt
    14761 catatacatg aacgtcaaca attaagtgat atgcctgtaa ttaactattc aggtaatatt
    14821 caaggtagac attttaatga gcaaggtgaa aaaggttgct tattaatcga gggtgaccac
    14881 ttaaaattaa agactaaatt ttatcctaca cagtatatta gatttgaaga agcaactatt
    14941 gaaacggata agacatctaa gcaaggttta tacgaggtca ttcaaaactt taaagaacaa
    15001 gtgagagaag aaggaaaagc cttttatcgt ttaacgcttg ttattaatag tgagacatta
    15061 atttcacctc aagatttatt acaagttgaa gaaatgatta cagattatga agaaaacgaa
    15121 aatcaatttg tatatattga tgagttaaaa atacaatatg cacaaaatga tgagtcacct
    15181 ttagttaatg aattttcagc ggaattatta gtcgatcaaa ctgtttttga taaagcgatg
    15241 tcagatttat atttaaatcc aagggcatct aagttcctag acgattatgg aacattcgac
    15301 catacagcat tagttaatcg tgctgaagaa atattaaaag ctgaaatgag aggtgaacaa
    15361 aatgataatt aaatcacttg aaatttatgg ttacggtcaa tttgttcaac gtaaaattga
    15421 atttaataaa aacttcactg aaatttttgg tgaaaatgaa gcgggtaaat cgacgattca
    15481 agcattcatc cattcgatat tatttggatt tccaactaaa aagtctaaag agccaagact
    15541 agaaccacgt ctaggtaacc aatacggtgg taaattagta cttattcttg atgatggctt
    15601 agagattgaa gttgaacgaa ttaaaggcag tgctcaaggt gatgtgaaag tatatttacc
    15661 taatggtgct gtgcgtgatg atgcttggtt acaaaagaaa cttaattata tttctaaaaa
    15721 gacatatcaa ggtatctttt catttgatgt actagggctt caagacattc atagaaatct
    15781 aaatgaaaaa caattgcaag attatttatt acaagcaggg gctttaggat caactgaatt
    15841 cacgtcaatg cgcgaagtga ttaatcgtaa aaaagatgaa ttatataaaa aatcaggtaa
    15901 aaatccgatc attaatcaac aaattgagca attaaaacaa ctagaaagtc aaattcgtga
    15961 agaagaagca aagctagaaa catatcatcg cttagtagat gatcgagata aatcatcacg
    16021 tcgattagag aatttaaagc ataatttaaa tcaattatca aaaatgcatg aagaaaaaca
    16081 aaaagaggtt gctttacatg atcattcaca agaatggaag tctctagaac aacagttaaa
    16141 tattgagcca atcacattcc cagaaaaagg tgtggatcgt tacgaaaaag cacgagcgca
    16201 taagcaatcg ttagaaagag atattggttt aagaaatgag cgtttagctc aacttaaaga
    16261 agaagcgact caattagagc cagttaaaca atctgatatt gacgccttca ttagtttgaa
    16321 tcaacaagaa aatgaaatta aaaataaaga atttgaactt actgcaatcg aaaaggatat
    16381 tgcgaataaa caacgtgata aagatgaatt gcaatcaaat attggttggt ctgaaacgca
    16441 tcatgacgta gatagttcag aggcaatgaa aagttatgtc agtgagcaaa tcaagaataa
    16501 acaagaacaa gctgcataca ttaaacaatt agaacgtagt ttagaagaaa ataaaatcga
    16561 agataatgcg gttcatagcg aactagattc tgttgaagaa aaaatagttc ctgaagagac
    16621 ttttgaaaag aaaaaagaat actcacaaca agtcattgaa ttaaacgaaa aagagaactt
    16681 gtatagcaaa ttgaaagaac gtttcgaaat tgagcaacaa gaaaaacaaa aacgtcaaaa
    16741 attgttacga acaacgttta ttcttttaac attagtaggt attggtttaa ctgccttctc
    16801 atttatttca aacaacatgt tgtttggtat tattttcgca gtattaactt tagtatttgt
    16861 tatcggtatc atcatgtcta aatcaaaaga agtagattat agcgaggcca tcacagatga
    16921 aatcgaagaa attaaagcac aactagccat tttagatgaa aattatgact tagattttga
    16981 tcttgatgag caatatcgca ttcgtgatca ctggcaacaa gctttgaaaa ataaagatat
    17041 tttagaagag aaacgtcagt atattgaagg tcgtttaaat gatgcgaaag ggcgtcatga
    17101 tgaattacaa tcgacagttg agaacgttaa ggatgaatta tacttatcat ctaaaatttc
    17161 aaatgattta attgttgata gtatttctac aatggcgaat attaaagcat tagatcaaca
    17221 tatcagtgat ttaaatcaac aacgccagca attggttcaa gaattagata cattctacaa
    17281 tcatgctgaa gctgttacaa aatcacaatt tgtttatttc aacaaattat ctttattcca
    17341 cgatgtgcaa caatggttga aatcagctga agacacgaat gaaaaatggc gtattaatgc
    17401 tgaaaatacc aaacttgtta caaatgaatt aaatcattta aatgctcaat tagaagaaaa
    17461 taataaagaa attacagcac tattcgattt tatcaatgtt ggtactgaag aagattttta
    17521 ccaacatcat gaagattacc aaacttatac tagtaatttg agtcgtttta atgatttaac
    17581 taaatatctt gagaaccaaa actattctta tgaattaagt tctagtttaa gtgaaaaaac
    17641 aactgcacaa cttgaagaag aagatcattt attggctact caagttgacg aatataatga
    17701 gcaatatctt gaaatgcaag cacaagtcag tgatttaagt gcacaaatca atcacatgga
    17761 aactgataca acgcttgcta atttaagaca tgaatatcac agtcttaaaa atcaacttaa
    17821 tgatatcgca aaagattggg caagtttaag ttatttacaa agtttagttg atgaacacat
    17881 taaacaaatt aaagataaac gtttgcctca agttattaat gaagcggtag aaatattgaa
    17941 gcatttaaca gatggcagat atacgatgat taactataat gaagattcaa ttacggttaa
    18001 acatgttaat ggtcaattat atgatcctgt tgaactaagt caatctacaa aagaattact
    18061 ttatgtagct ttacgtatca gtttaattaa ggtactaaga ccatattatc cgttcccatt
    18121 aattgttgat gatgcatttg ttcattttga taaaaaacgt actgaaaaaa tgttgaatta
    18181 tttacgatca ttatcagaac actatcaagt actatacttt acatgtgtaa aagataatat
    18241 tgttccatca aaagaagtga ttacattaaa caaaatagag gaaggcggga aacgatgaga
    18301 aatatagaga atctaaatcc cggagattca gttgatcact ttttcttagt gcataaagct
    18361 acacagggtg taacagcaca aggtaaagat tatatgacat tacatttgca agataaaagt
    18421 ggtgaaattg aagcgaaatt ttggacggct acaaaaaatg atatggcaac aatcaagcct
    18481 gaagaaattg tacatgttaa aggtgacatc ataaactatc gcggaaataa acagatgaaa
    18541 gtcaaccaaa ttagactagc gacaactgaa gatcaattaa aaacagaaca atttgtagat
    18601 ggtgcacctt tatcaccggc agaaatacaa gaagagattt ctcattattt gctagatatt
    18661 gaaaatgcta atttacaacg tatcacacgt catttattga aaaaatatca agaacgattt
    18721 tacacatatc cagctgctag ttctcatcat cataactttg cgagtggctt aagctatcat
    18781 gtattaacga tgttacgtat tgcaaaatca atttgtgaca tttatccatt gttaaacaaa
    18841 agtttgttat atagtggtat tattttgcat gatattggta aagttagaga attgagtggt
    18901 cctgttgcga cgtcgtatac agtcgaaggt aacttattag gacacatctc gattgcgagt
    18961 gatgaagtag ttgaagcagc tcgtgaattg aacattgaag gagaagaaat catgttgtta
    19021 agacatatga ttttatctca tcatggtaag ttagagtatg gttctccaaa actgccatac
    19081 ttaaaagaag cagaaatttt atgctatatc gataatatcg atgctagaat gaatatgttt
    19141 gaaaaggcat ataaaaaaac tgacaagggt cagtttacag ataaaatatt tggtcttgaa
    19201 aatcgtagat tctacaatcc tgaatcactc gattaagaat taaaagatat cggacagatg
    19261 agtatactga aacatgagct catctgtccg atttttaatg cgaaaaaacc gagcggccgt
    19321 ggtatgattt ttgaaccacg gtcgctcggt tttgtgttat tggctcatgc cggattgtcc
    19381 gccttgtgcg ccaccttgtt taagtttttc agggtttaag attttatctt cgacaactga
    19441 tttaatatca cgatctttaa agtcaacatc gtattctttt aatagatctt tgtatgcatc
    19501 agtcaataat tttggatttt tttgtacttt ctgatcgact aatttttctt tcaggctttg
    19561 tttttcactg ttaaagtctg ttggtttatc agctttaata atatgatatc caaagcttga
    19621 tttaacaacc tctgatactt caccatcttt aagcttaaat agtgcttttt caaaatcttt
    19681 atcagtttgt cctttaagaa cataacctaa ttcgccatct tttttagctg aaccagtatc
    19741 cattgattct tttttagcga tttcaccaaa tttacttgga tcttttgaaa cttctttttg
    19801 aatttcttca gctttttgtt tcgcttcttt atcatctaag ccttctttgt cgcttttctt
    19861 agatttaact ttaattaaaa tgtgtgaagc tttcttgctg tcttctttaa tttcagaatc
    19921 agagatttta attttatctg atagtaattc tttatgataa gcagcagtac gtaaattttc
    19981 tttatattta tcggctgtta aaccttgctg ttgaagggcc ttttcaaatt tatctttacc
    20041 gccgtattgc ttttgcattt tttcaatttg ttcgtcaatc ttcttatcat taactttatt
    20101 tttatattta tcagctaaaa ttttatttaa catttcagta aatgatgcat ttgcaatttg
    20161 atctttaccg atttttttca ttgtatctgc aactgttacg tctccagctt tagaagaaat
    20221 taatgtattt tctttagagt ctgtggcact agcgccacaa gcgcctaata ataaagcact
    20281 agctgttacc ggaacgatta atttgtttat catcttcata gttgaaactc ctttgtaagt
    20341 taatcatcgt gaatataaca tagttttata ttatcaccaa tcaataaccc atgacatcag
    20401 actagagcat taggtttagg ggtagaaatt gatgaaaata aaaaaacaag gtctatatac
    20461 atttgattgt aagctattac atagcctaaa aacaaataat aacaccttgc tataattatt
    20521 ttgttataaa tgatatggat ttttaatttt tttcaaaat
//
LOCUS       DAMQYT010000023        19727 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMQYT010000023 DAMQYT010000000
VERSION     DAMQYT010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19727
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.023"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(86..952)
                     /gene="galU"
                     /locus_tag="PGA33_000717"
     CDS             complement(86..952)
                     /gene="galU"
                     /locus_tag="PGA33_000717"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDG6472550.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKALGL"
     gene            complement(1132..3954)
                     /gene="fnbB"
                     /locus_tag="PGA33_000718"
     CDS             complement(1132..3954)
                     /gene="fnbB"
                     /locus_tag="PGA33_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HDG6472551.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPKTVQAP
                     KVETSRVDLPSEKVADKETTGTQVDIAQPSNVSEIKPRMKRSTDVTAVAEKEVVEETK
                     ATGTDVTNKVEVEEGSEIVGHKQDTNVVNPHNAERVTLKYKWKFGEGIKAGDYFDFTL
                     SDNVETHGISTLRKVPEIKSTDGQVMATGEIIGERKVRYTFKEYVQEKKDLTAELSLN
                     LFIDPTTVTQKGNQNVEVKLGETTVSKIFNIQYLGGVRDNWGVTANGRIDTLNKVDGK
                     FSHFAYMKPNNQSLSSVTVTGQVTKGNKPGVNNPTVKVYKHIGSDDLAESVYAKLDDV
                     SKFEDVTDNMSLDFDTNGGYSLNFNNLDQSKNYVIKYEGYYDSNASNLEFQTHLFGYY
                     NYYYTSNLTWKNGVAFYSNNAQGDGKDKLKEPIIEHSTPIELEFKSEPPVEKHELTGT
                     IEESNDSKPIDFEYHTAVEGAEGHAEGTIETEEDSIHVDFEESTHENSKHHADVVEYE
                     EDTNPGGGQVTTESNLVEFDEDSTKGIVTGAVSDHTTIEDTKEYTTESNLIELVDELP
                     EEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYEGGQ
                     NSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNNGNQSFEEDTEKDKPKYEQ
                     GGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTLPQVSGHN
                     EGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPTEPGK
                     PIPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVVPTKKAQSKKSELPETGGEESTN
                     NGMLFGGLFSILGLALLRRNKKNHKA"
     gene            complement(4636..7692)
                     /gene="fnbA"
                     /locus_tag="PGA33_000719"
     CDS             complement(4636..7692)
                     /gene="fnbA"
                     /locus_tag="PGA33_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HDG6472552.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAP
                     QTAQPANIETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVE
                     VAQPRTASESKPRVTRSADVAEAKEASNAKVETGTDVTSKVTVEIGSIEGHNNTNKVE
                     PHAGQRAVLKYKLKFENGLHQGDYFDFTLSNNVNTHGVSTARKVPEIKNGSVVMATGE
                     VLEGGKIRYTFTNDIEDKVDVTAELEINLFIDPKTVQTNGNQTITSTLNEEQTSKELD
                     VKYKDGIGNYYANLNGSIETFNKANNRFSHVAFIKPNNGKTTSVTVTGTLMKGSNQNG
                     NQPKVRIFEYLGNNEDIAKSVYANTTDTSKFKEVTSNMSGNLNLQNNGSYSLNIENLD
                     KTYVVHYDGEYLNGTDEVDFRTQMVGHPEQLYKYYYDRGYTLTWDNGLVLYSNKANGN
                     GKNGPIIQNNKFEYKEDTIKETLTGQYDKNLVTTVEEEYDSSTLDIDYHTAIDGGGGY
                     VDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHA
                     DVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESNLIE
                     LVDELPEEHGQAQGPVEEITENNHHISHSGLGTENGHGNYDVIEEIEENSHVDIKSEL
                     GYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGNQSFEEDTEKD
                     KPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPSYQFGGHNSVDFEEDTLP
                     KVSGQNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEV
                     PSEPETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPT
                     KKPQSKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNHKA"
     gene            complement(7676..7777)
                     /locus_tag="PGA33_000720"
     CDS             complement(7676..7777)
                     /locus_tag="PGA33_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472553.1"
                     /translation="MLLKTEQYRLAFIKFKKLMNFAFKGRYYSEKQS"
     gene            complement(8161..9519)
                     /locus_tag="PGA33_000721"
     CDS             complement(8161..9519)
                     /locus_tag="PGA33_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HDG6472554.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(9636..11189)
                     /gene="gntK"
                     /locus_tag="PGA33_000722"
     CDS             complement(9636..11189)
                     /gene="gntK"
                     /locus_tag="PGA33_000722"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDG6472555.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVEALELLGISKEMLPELVPTTYVMKGM
                     KERYATLMGLNKDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPIEENVTVYQEIVSIFINLSRSLTENYEQIADFQR
                     QHIAENKTQ"
     gene            complement(11213..11893)
                     /locus_tag="PGA33_000723"
     CDS             complement(11213..11893)
                     /locus_tag="PGA33_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDG6472556.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(12047..12811)
                     /locus_tag="PGA33_000724"
     CDS             complement(12047..12811)
                     /locus_tag="PGA33_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDG6472557.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISA
                     ILMKNKWPFLIALPFMIGYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            complement(12976..13668)
                     /locus_tag="PGA33_000725"
     CDS             complement(12976..13668)
                     /locus_tag="PGA33_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDG6472558.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(13899..14315)
                     /locus_tag="PGA33_000726"
     CDS             complement(13899..14315)
                     /locus_tag="PGA33_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDG6472559.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            complement(14454..16277)
                     /locus_tag="PGA33_000727"
     CDS             complement(14454..16277)
                     /locus_tag="PGA33_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDG6472560.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKSAFLQVAKTAVDSFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSEENVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLAIFENIAK
                     HFELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
     gene            complement(16755..18032)
                     /locus_tag="PGA33_000728"
     CDS             complement(16755..18032)
                     /locus_tag="PGA33_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDG6472561.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            18579..19190
                     /locus_tag="PGA33_000729"
     CDS             18579..19190
                     /locus_tag="PGA33_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDG6472562.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            complement(19199..19297)
                     /locus_tag="PGA33_000730"
     CDS             complement(19199..19297)
                     /locus_tag="PGA33_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472563.1"
                     /translation="MPNKLKKAVNHFNRWNDSLLFVYRNLKFMPLC"
     gene            complement(19287..>19727)
                     /locus_tag="PGA33_000731"
     CDS             complement(19287..>19727)
                     /locus_tag="PGA33_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDG6472564.1"
                     /translation="SGVLFLPITVSVILGSLFYKRFSKKYNEIMILRVTITGFAIFTL
                     LFGWLNESNVIILSVIIFILGTCVGIVPALLSTIISKRFEHIKGKVLGVFNFVRYIGM
                     TVGALLIGIISQPLVAFYFTTITIMLIVIFLYIKIVDFQLKYAK"
BASE COUNT         5784 a         3826 c         2891 g         7226 t
ORIGIN      
        1 cacaacacat aaattaagca tgacactcat tcaatttagt tcaccatttc gtgttccaat
       61 tttactgagt atcatgcttt taatgttata aacctaatgc tttaataaat cgtgttaatt
      121 cttctcgcat actgtcatct ttcaatgcat attctatggt agttttaacg aagcctaatt
      181 tttctccaac gtcataacgt tcgccttcga agtcatatgc atacacttgg ttatcattat
      241 tcatacgttc aatcgcatct gttaactgaa tttcgttacc tgcgccttct ttttgcgttt
      301 ttaaataatc gaaaatttca ggcgttaata cataacgtcc cataatagct aggtttgatg
      361 gtgccgtacc ttgtgctggc ttttcaacaa actttttcac ttcatactga cgtccgtttt
      421 tagttaatgg gtcaataatt ccataacgat gagtatctgc ttccggaact tcttggacac
      481 ctataactga gtgccctgtt tcttcataaa cgtcaatcaa ctgtttcact gctggcactt
      541 cagattcaac aatatcgtca cctaataata ccgcaaatgg ttcattgccg ataaattgac
      601 gcgcagaact aatcgcatgc cctaaacctt tttgttcttt ctgccttaca taaaaaatat
      661 tcgcaagttc cgttgaatac tgaactttct ctagtaattc agatttacct ttttctttta
      721 acaccatttc taattctttt tgactatcaa aatgatcttc aatcgcgcgt ttgtggcgac
      781 ctgtcactat aataatatct tcaattccag ctcttgcagc ttcttcaacg atatattgta
      841 ttgtgggttt atctaagata ggaagcattt cctttggcat cgctttagtt gctggtaaaa
      901 atctagtccc taaaccagca gcgggaatga ttgccttttt tatttttttc aaagttaatg
      961 tgctcctttt cctaagtatt aaatctatgt atcaacgtca ttttaacact aattagaacg
     1021 ccttcatagt gtcattgagt atgtaattat ttcttgggaa atttgtttta attttaaaaa
     1081 acaggcttac ttcatataat ttatgaaata aacctgtcaa ttttggattg attatgcttt
     1141 gtgattcttt ttatttctgc gtaataacgc taaacctaaa atgctaaata atccgccgaa
     1201 caacatgccg ttgtttgttg attcttctcc acctgtttca ggtagttcag atttcttaga
     1261 ttgtgctttt ttagttggta ccactgcttt aaccttttca ttgatttcaa taacaggtgt
     1321 tactacttta ccttgttcca ctggtttaga aggtttttta ggttcttctt tagcaggtgg
     1381 tattggttta ccaggttcag ttggtacctc tggcgttggc ggtgttggtg tttccggctc
     1441 gcttggtact tctggtgtcg gtggtgttgg tgtttccggc tcgcttggta cttctggtgt
     1501 cggtggcgtt ggtggcacga ttggaggtgt tgtatcttct tcaatcgttt gttgaccttc
     1561 attatgacca cttacttgtg gaagtgtatc ttcttcaaag tcaacactat tgtgtccacc
     1621 gaattgataa tttggtttat ctttatttgt atcttcttca ataatttcag tgtgcttatt
     1681 gaatccgtga atatgtggca cactgtcgaa gtcgatatca atgatattac caccttgttc
     1741 atacttaggt ttgtctttct ctgtatcttc ttcgaatgat tggttaccat tattttgacc
     1801 atgaatttga ggtacactat cgaaatcgat atctacgata ttgccacctt gttcatattt
     1861 cggtttatct tcttctgtgt cttcctcaaa tgactgatta ccgctatttt ggccaccttc
     1921 gtaacctaat tcactcttaa tatccacgtg gctattttct tcgatttctt caatcacgcc
     1981 ataattaccg tgaccatttt cagttcctaa accagaatga gaaatatgat gattgttttc
     2041 agtaatttcc tcgattggtc cttgcgcttg accatgttct tcaggtagtt catctactag
     2101 ttcaatcaga ttactttcag tcgtatattc tttcgtatct tcaattgttg tatgatcgct
     2161 aacagcacca gttacaatac cttttgtaga atcttcgtca aattcaacta ggttagactc
     2221 agtagtaacc tgaccaccac ctgggtttgt atcttcttca tattcaacaa catcagcatg
     2281 atgttttgaa ttttcatgtg tcgattcttc aaagtctaca tgaatagaat cttcttcagt
     2341 ttcaatggta ccttctgcat gaccttctgc accttcaaca gctgtatgat attcaaaatc
     2401 aattggctta gaatcattac tttcttcgat tgtaccagtc aattcatgct tctccactgg
     2461 cggctctgat ttaaattcaa gttcgatagg agtactatgt tctataatag gttcctttag
     2521 tttatctttg ccgtcgcctt gagcgttatt agagtaaaat gcaacgccat ttttccaagt
     2581 taaattactt gtataataat agttataata tccaaaaagg tgtgtttgaa attctaagtt
     2641 gctagcattt gaatcataat acccttcata ttttattaca taatttttac tttggtctaa
     2701 attattaaag tttaaagaat aaccaccatt agtatcaaaa tctaaactca tattatcagt
     2761 cacatcttca aatttgctga catcatcaag ctttgcatat acgctttcag ctaaatcgtc
     2821 tgaaccaatg tgtttatata ccttaactgt tggattatta acccctggtt tatttccttt
     2881 agttacttga ccagttactg tcacagagct taacgactgg ttgttaggtt tcatgtacgc
     2941 aaaatgacta aatttcccat ctactttatt taaagtatca attcgaccat tagctgttac
     3001 tccccaatta tctctaactc cacctaaata ttgaatatta aatattttgc taaccgtagt
     3061 ctcacccaat ttaacttcaa cattttggtt acctttttgc gtcactgttg taggatcaat
     3121 aaatagattt aaagataatt cagcagttaa atctttcttt tcttgtacat attctttaaa
     3181 cgtatatcta acttttcttt ctccaattat ttctcctgtc gccataactt gaccatctgt
     3241 actttttatc tccggaactt tacgcagtgt tgagatacca tgagtttcaa cattatcgct
     3301 taatgtgaaa tcaaaataat ctcccgcctt aattccttct ccaaatttcc atttatattt
     3361 caaggttact ctttctgcgt tatgaggatt tacaacattc gtatcttgtt tatgtcctac
     3421 aatttcacta ccttcttcta cttccacttt atttgttaca tctgtacctg tcgctttagt
     3481 ttcttccact acttctttct ctgcaactgc tgtaacgtca gttgatcttt tcattcttgg
     3541 tttaatttct gagacgttac ttggttgagc tatgtcaact tgagttcctg tagtttcctt
     3601 atcagcaact ttttccgatg gcaaatcaac tcgcgaagtt tctacttttg gtgcttgcac
     3661 agttttcggt gcttcttctg ttgttacttg tgttgattgt gatggttgct cagttgatgt
     3721 cgcgctgtat gattgtgttt catctattgt attaacgtta tttgtagttg tttgtgtttc
     3781 gcttgcttta ctttcagtag ctgaactccc actttcctct actgtagtat tgttttgttc
     3841 cgatgctgca gcttcttttt cttgtcccat tccaacaacg atcattgttc ctaagaatac
     3901 tgaggccgct cccaatttgt gttttcttat gccgtatcta agattgcttt tcactataat
     3961 attctccctt aaatgcaaaa ttcatttatt tttaaaactc aataaatgca attctatatt
     4021 gttcggtttt taaaagcaat gaaaaaaagc gagttaataa aaagttaaga ttgttgttaa
     4081 ctttatgtat aatgagtttt ttattatttg aaactcacat atatattgca tacaaagctc
     4141 ttgaacacct tgatataaca ggcttgtatt ttttatactt actttttaaa ttaaattcaa
     4201 attcatctaa tttaaaacaa tatactaaac catacataat aatcgcctgt acaatgcatc
     4261 attaacaagt cactgaaacg cctttcattg tattaataac gtcactataa tttttatatc
     4321 gttcggtttt tgtttgattt taatgattat ttatacaaaa acagccgtat ttcaagccga
     4381 cattttaaat ttaactaaat ttgcatctag ttaataattg catttatcaa atttgtctta
     4441 ttgatccaat ctaatttgta ctcacaaact agtttaaaat tctaacttta tctctcagtt
     4501 cgttatcaat catcagacat aaaccaatga agcaatcaga aaacactcta attttctatt
     4561 agaaatttga tttaatataa aaaaacaggc ttacttcata taatttatga aataaacccg
     4621 tcaatttttg tttaattatg ctttgtgatt ctttttattt ctgcgtaata atgctaaacc
     4681 tagaatgctg aataatccgc cgaacaacat acctttgttt gttgattctt ctccacctgt
     4741 ttcaggtagt tcagatttct tagattgtgg ttttttagtt ggtgccactg ctttaacctt
     4801 ttcattgatt tcaataacag gtgttactac tttaccttgt tccactggtt tagaaggctt
     4861 tttaggttct tctttggcag gtggtactgg tttaccaggt tcagctggta cctctggtgt
     4921 tggcggtgtt ggagtttctg gctcactcgg cacttctggt gtcggtggtg ttggtgtttc
     4981 cggctcactt ggtacttctg gtgttggtgg cgttggtgtt tccggctcac ttggtacttc
     5041 tggtgtcggt ggcgttggtg gcacgattgg aggtgttgta tcttcttcaa tcgtttgttg
     5101 accttcattt tggccgctta cttttggaag tgtatcttct tcaaagtcaa cactattgtg
     5161 tccaccgaat tgataacttg gtttatcttt atttgtatct tcttcaataa tttcagtgtg
     5221 cttattgaat ccgtgaatat gtggcacact gtcgaagtcg atatcaatga tgttaccgcc
     5281 atgttcatac ttaggtttgt ctttttctgt atcttcctcg aatgactgat tacctttatt
     5341 ttgaccatga atttgaggta cactatcaaa atcgatatct acgatattgc caccttgttc
     5401 atatttaggt ttgtcttctt ctgtgtcttc ctcgaatgac tggttaccgc tattttggcc
     5461 accttcataa cctaattcac tcttaatatc aacgtggcta ttttcttcga tttcttcaat
     5521 cacgtcataa ttcccgtgac cattttcagt tcctaaacca gaatgagaaa tatgatgatt
     5581 gttttcagta atttcctcga ctggtccttg tgcttgacca tgctcttcag gtaattcatc
     5641 cactaattca atcagattac tttcagttgt atattctttc gtatcttcaa ctgttgtatg
     5701 atcgctcact gcgccagtta caataccttt tgtagactct tcgtcaaatt caactaagtt
     5761 agactcagta gtaacctgac caccacctgg gtttgtatct tcttcatatt caacaacatc
     5821 agcgtgatgt tttgaatttt catgtgtaga ttcttcaaag tcaattggat ttgattcctc
     5881 agaggactca gtgtatcctc caacgtgacc tgcttcgcta tccacagcag tatggtaatc
     5941 gatatcaata gctgatgaat ccgtttcttc tattgtttca atgtatccat caacatatcc
     6001 acctccacca tctatagctg tgtggtaatc aatgtcaaga gttgatgaat catattcctc
     6061 ttcaacagta gttactaaat tcttatcata ttgacctgta agagtttctt taattgtatc
     6121 ttctttatat tcaaatttat tattttgaat aatcggacca tttttcccat ttccgttcgc
     6181 tttattactg tataaaacta aaccattatc ccaagttaag gtatatcctc tatcataata
     6241 atacttataa agttgctctg gatgtcctac catttgtgtt ctaaaatcaa cttcatcagt
     6301 accatttaaa tactctccat catagtgaac aacataagtt ttatctagat tttctatatt
     6361 caatgaatag cttccattat tttgtaaatt caaattccca ctcatattac ttgtgacttc
     6421 tttaaattta gaagtatctg tcgtatttgc atatacactc ttcgctatgt cttcattatt
     6481 acccaagtat tcaaatatcc taacttttgg ttgatttcca ttctgattac tacctttcat
     6541 taaagttcca gtaacagtca cacttgtcgt tttaccatta ttaggtttaa taaatgcaac
     6601 atgcgaaaat ctattattcg ctttattaaa tgtctcaatc gatccattta aattggcata
     6661 ataattccca ataccatctt tatatttaac atctaattcc tttgaagttt gttcttcatt
     6721 tagtgttgaa gttatagttt gatttccatt agtttgtaca gttttaggat caataaataa
     6781 attaatttct agttcagccg ttacatcaac cttatcttca atatcatttg taaatgtata
     6841 tctaatcttt ccaccttcta aaacttcacc tgtcgccatt acgactgaac catttttaat
     6901 ttctggtact tttctagcag ttgatacgcc atgcgtattt acattatttg ataaagtaaa
     6961 gtcaaagtag tcaccttgat gtaaaccatt ctcaaatttc aacttatatt ttagtaccgc
     7021 tcgttgtcct gcatgaggtt ctactttatt tgtattgtta tgcccctcaa tagaaccaat
     7081 ttctactgta actttacttg ttacatctgt acccgtttcc actttcgcgt tactagcttc
     7141 cttagcttcc gctacatctg ctgatcttgt cacacgtggc ttactttctg atgccgttct
     7201 tggctgtgcc acttcaactt gtgtttctgc gacttgattt tgtgtagcct ttttaggtgt
     7261 taaatctact tgtctttgat ctccgctatt gtcttgagat tgtgttgttt ccttaacttg
     7321 aggtttcgct tcttccttaa ctacctcttc tttaactgtt tctatatttg ctggttgtgc
     7381 agtttgtggt gcttgtactg cttttggtgc ttcttcagtt gttacttgtg ttgcgtttga
     7441 cggttgttct gttactgttg cgttatatga ttgagtttct tctatatgat taacgttagt
     7501 tgcagttgtt tgtgtttcac ttgttttatt atcagtagct gaattcccat tttcttctac
     7561 tgtagttgtc ttttgttctg atgctgcagc ttctttgtct tgtcccatcc caacaacgat
     7621 cattgttcct aagaatactg atgctgctcc caatttatgt tttctaatgc cgtacctaag
     7681 attgtttttc actataatat ctccctttaa atgcaaaatt cattaatttt ttaaacttaa
     7741 taaatgcaag tctatattgt tcggttttta aaagcaatac aaaaaagcga gttaatgaaa
     7801 agttaagatt gtcattaatt ttatgttaat tgataatttt attatttgaa atatacctat
     7861 aaattgtatt caagtcatca gaaacccttg tcacacaagg cttgtatttt ttatacttat
     7921 ttttttaaat taaattcatc attatctaat ttaaaacaat atactaaacg tttcataatt
     7981 atcgcctgta caatacgcac aaaaacatgt cttgaaacgc ctttcatcac tctaaaatac
     8041 ccaatatact ttttatatcg ttcggattct gagtatttca gacgattttc tgcataaaaa
     8101 taaacgtgtt tcaaggcaat atattgcaat tacctaaaaa cacgtttact taatatttag
     8161 ttaaacaaat aagctaatga ataaaatgaa gatgatacct gaaacggaaa taatcgtttc
     8221 taataatgac catgttaaga atgtttcttt tacagttaaa ccaaaatatt ctttaaacat
     8281 ccaaaatcct gcgtcattta catgagacaa aatcacacta cctgcaccta tcgcaagtac
     8341 aactaatgca acatttacat ctgatgattg taataatggt aagacaatac ctgtagttga
     8401 aatcgcagct actgtagccg aacctaatgc gatacgtagc acagctgcaa caatccatgc
     8461 tagtaaaatc ggagacatct ctgtaccttc aaacatttta gcaattgtat ttccgacacc
     8521 gccgtcaatt aatacttgtt taaatgtacc gccaccgcca ataatcaata acatcattcc
     8581 gattggataa atcgcattcg tcactgattc cataatatga ttcatcttac gctttctcat
     8641 taatcccatc gtaacgattg caaataatac tgctattagc atggctgtcc ctgctgttcc
     8701 tatcatataa atgatagatt caaatagatt tgtaggtttg tcatgcccag ttacaagttg
     8761 cgttatcgta gacactaaca ttaatatgac tggtaatgtt gctgttaata aactcatacc
     8821 aaatcctggc atctcttgat ccgtaaattc tttttgtgca cctaacgctg aaatatcgcc
     8881 ttctcgtgta tacgcagacg gaatcatttt ttgtgcaact ttgttaaata taggccctgc
     8941 aatgagtgta actggaatgg caataatcat accatacagt aatacatctc caacatttgc
     9001 ctttaattct tttgcgatga ctaccggtcc tggatgtggt ggtaaaaagc catgtgtcac
     9061 tgataaagct gttaccatag gtagtcctag ttttaacact gaaacatttg cgcgttttgc
     9121 tactgtaaat actaatggaa tcagtaagac taaacctact tcaaagaaca atgcaatacc
     9181 gacgataaat gctgcaacaa gcattgccca ttgtacatgt ttttgaccaa atttttgaat
     9241 caacgtgtct gcgattcgag ttgcaccacc accatcagca agcaatttcc caagtatggc
     9301 acctaaaccg aatatcagtg caatgtggcc gagcgtactg cccattcctt tctcaatcgt
     9361 ctccataatt ttagtcaatg gtatacctag cattaacgct gtaatcatcg atgtgataat
     9421 taatgaaata aatgtattta atttaaaccc aataattaat actaataaaa taacgatacc
     9481 taaaacaaca ctgattaacg gccatatttc gttaaacatg acattcccct ctttctcttt
     9541 tcaatagaat gtaacaccgt cgtcgcgcac gtattccaca taatatatag tagtcatggc
     9601 acaacaactt tatctaagat gcttgagtga cgtatttatt gtgttttatt ttcagcgata
     9661 tgttggcgtt gaaaatctgc aatttgttca taattctctg ttaaagaacg acttaaattg
     9721 ataaaaatgg atacgatctc ttggtaaaca gtgacatttt cttcaatcgg cgtatgattg
     9781 tttgtggcac cgaccatcga tgaaacgatt gaaaaatctt caatgtcacc tacagcttta
     9841 agtccgagca cgcaggcacc taagcatgaa ctttcataac tttcaggaac cactaactct
     9901 gtgtcaaata tatctgacat catttgacgc catacttcac ttttcgcaaa accacctgtt
     9961 gcttttatca tcttaggtgt ttcattcatt acttcaataa gcgcaagata gacggtatac
    10021 aaattgtaaa gaacaccttc taatgcagcg cgaatcatat gttctttttt atgagataaa
    10081 gttaaaccga agaatgaacc tcttgcattt gcgttccaaa gcggcgcacg ttctcctgct
    10141 aaatagggat ggaatattaa accatctgca cctggtttaa cacgctttgc aatttgagtt
    10201 aagacatcat aaggatcaac accgagacgt ttcgcagttt cgacttcact cgctagcaac
    10261 tcgtcgcgca accatctcaa tacgacacca ccattattta caggacctcc gatgacgtag
    10321 tggtcctctg ttaagacata acaaaatatt ctacctttgt aatcagtacg cggtttatct
    10381 atcacagtac gaatcgcccc agatgtaccg attgtgacag caacttctcc tttaccaaca
    10441 ctattgacac ctaaattaga aaggacccca tcactcgcac caataacaaa cggtgtatct
    10501 ttattaagcc ccattaatgt tgcataacgt tctttcatac ctttcatcac atacgttgtt
    10561 ggaactaatt ccggcaacat ttccttggaa atacccagca gttctaatgc ctcaacatcc
    10621 caatctaatg tttctaaatt aaacatccct gttgcggaag ccattgaata atcaatgata
    10681 tatgtatcaa ataaatgata gaaaatgtat gttttaatat ctgcaaactt agcagtacgt
    10741 tgaaatacat cttgccattc atgtttcatc caaaaaatct tcgctaatgg cgacatagga
    10801 tgaatcggtg tgcctgttcg ctggtaaatc gcattgccat catgcacttc atttattact
    10861 gttgcatatt ttgcagcgcg gttatctgcc caagtaatat tatttgttaa tctttgatgt
    10921 tgctgatcca tcgcaatcaa gctatgcatt tgcgcactaa atgacacaaa cttaatgtcg
    10981 tctttattaa ctttggattc tctcataaca tatttaatag tcattagtac tgcatcaaat
    11041 aattcatctg ggttttcttc tgagacatca acgtttggtg tgtgtaaatc atagcctatt
    11101 tgatgtttca tgataaaagt tccattttca tcatataaga ctgacttggt actcgtcgtt
    11161 ccaatgtcga caccaatcat atatttcatg ataaatcctt ctttctttca ttttaattca
    11221 accaaaatcc ttcaatatct ttaccaacat cgtcgaaatt taaatgaaac gcttctttca
    11281 aaatttgact gtcgtattgt tccactgcat caataaacac ttgatgatta tgatgtatgc
    11341 gttcaaaatc ttgcgggttc tgttgcatac gttgtcgcat ggatgttaaa acgagtgcca
    11401 tcattactgg ttttaaatga ctccaaaatg agtttaaata ttgatgatta gatgctttga
    11461 ttaatgtttc atgaaattca aagtcatgct tcgtaaatga ttctgcatcc tcaaatttta
    11521 ctgccacttt catcatttca agttgtttct tcatttcttt tacgatgggt agtcgctctt
    11581 gatttttaac tcttgaaaat gcaaatgact ctaacatcag tcgcaaatca tacatttctt
    11641 tcttttcttg ttccccaaac ggcaacacat gtgcacccat tctttctaat tggatgagtt
    11701 gattttgttg caataattta aatgcatctc gaattggcga acgactcaca ttaaattgct
    11761 ttgccatttg attttcagtg agtaacgtac cttcagctat gtgaccattc acaatgccta
    11821 agcgtaattc tgccgcgata ccttctccag ttgtcatacc ttccaaccat ttctctggat
    11881 atccatacat catcaaagtc actccttcat tacacgacat acttgtatac aagtatgtta
    11941 atatagttat tatgagtttg caagcgcttt ctttacgagc actaaaatag tgaccacccc
    12001 ttttcgattt aaatttaaag gaaatggtca ctatcacacg aatgatttaa ttgttatgtt
    12061 gtatgtggga tatttctaat tgttctgtac tcatatgcgc tttaggtact tcaatgcaat
    12121 aatgcgtttc atgacagttt ggacattcga atcgacgtgt tgtcgctgta tgtttcgctt
    12181 tgataactgc ccacaaagat ggtgagaata tatgctggca gttaggacat aaataggcaa
    12241 ccttttgttg gtaataaaaa gtaacaccaa tgccataacc aatcataaat ggtaaagcaa
    12301 ttaaaaacgg ccatttattt ttcatcaaaa ttgcacttat aatgctagaa tattgaatta
    12361 ttcctataat accagcacta atccaaatgt tacgacgaat acttttcatt tcagctgatt
    12421 tactcatgac atgctctatg tcttttaagt gtgtgattgg agacgtcgac gcttcattta
    12481 cgtaatattg aacattttta attttgttta ataccgcttg ttgctgttta acttgttggt
    12541 taatttcttg ttgtttcata gttagtaaag tattgagcgt cttcaaagta ccttcacctt
    12601 ttagcaacat atctatatcg cttaacgcac aacctaaatc tttaagcaat aagattaact
    12661 ctaatgtttg tcgctgttgt tctgtataca cacgacgctt tccttctgta aatccttgtg
    12721 gtttcaaaat acctttgcga tcataatatt gaatcgttcg tgttgtcaca ttgcataatt
    12781 ttgcgagttc tccagtcgaa tagttagaca tagattccac ctcctataat taccatagtt
    12841 gatgacccga cgtcacgagc aagtacaatt tccacatttt aaagaaattt attatactag
    12901 gcgtcttatt tttatgattt cgtaccatgt tgatttacaa actcactcaa actaagtaac
    12961 acacctacta aacatctact ctgttatttc agaatgaatt tgttgtaatt tatcttcaac
    13021 ttcagtaatc tctgtcgcac attctttcag taaatctcga tacttttccg tctctgcatt
    13081 gtttttataa cgtattttat gttctaaact tgcccacata tccataccta tcgttctaat
    13141 ttgaatttca acaggcaata cctcaacgcg ttctgctaaa aagactggaa tagatacaac
    13201 gatatgtaaa ctgcggtaac cattttcttt agggtgctga atataatctt tacgttttat
    13261 caattgtacg tcttcttgtt taagcaacat ctcttcaatc aaataaatat catctaagta
    13321 attacatact acgcgaattc cggcaatatc cagtatatgt tctttggcag tttctgcgct
    13381 aatctgtaat cctttacgat taagcttttc tattaaacta cgcatttctt tcacacgtcg
    13441 ctccatatga tgtataggat tgtgtttata aatgtgattg aaattgtcat ctaaaatact
    13501 cagtttagtg cttatttcct ttagtgctga cgaataaata tgatctaact ctacgaaacc
    13561 taataacgta tcaaatgctt catcaccatt ttcaaattta cttaagctat ttttaaaatc
    13621 atgtcgcaaa tcctctaaat atagtgatgg ttttcgatct acatacattt ttatactaac
    13681 ctccgatata taatcatctt tattgtacct aacattttat taagataata ctaatacact
    13741 atcgaacttt gggctgatac tgaaacagca caaagaaact caaacaattc gaattatgta
    13801 tcaaaacctt caattacagg aagtgatttt aaaatgattt aacgcaaaaa aacacctgtt
    13861 accgttatat aggtacaagt gcttaattga tagagatgtt atacgtcttt aaatgattcc
    13921 acaacttttg gatgtggacc atccataagc ggttctcttt gtcgaacgcc accactttgg
    13981 ttgccaattg attcgctatc aaagtacgat ttatcttgat ttgattgttc ttgaatatgt
    14041 tcttcattat cagttgttgc atattcacta tccacctctg ttttttccat tgttgctgta
    14101 tggaattgta cgaagttctc ttcttctaat gctttgattt cttctttcga taacgctcga
    14161 taccaatctt tcgctttttt cgccgcaata ggaacaacat ctttttcaag atatccttct
    14221 tttaacattg cattcgcaat ttctatcgct ttttgccgtt taattacttc aaattctttc
    14281 catttttcgg ggtaaccttt cattgtaaag ggcatttcct taacctccaa tatgttataa
    14341 ttcatgttat ataccaccat aattataaaa tgaaacatat taaacacatt tttttagact
    14401 cccgtcataa taacttcaga caaacgttag tctgacctga taaatattta attttaaacg
    14461 ttcttgtctc tctcacgaaa accattgtgt tgaatattca tcattgctga ttgaatataa
    14521 ttatactgtg gtaaatctgg taatatcttt atttctaatt ctgtatttaa ttcgaaatgc
    14581 ttagcgatgt tttcaaatat tgctaaatac tcgcccatta attgttcatt tattgtaagt
    14641 ctatcttcat tagccatggc tctatttaac ataaagctaa tttcttctaa tgcgaataag
    14701 ctaggataat aattttgaat caatgtctta tcactaaata attcaccatt agctgcatta
    14761 taaacttgcg tcatgttatt taatttcaca gataggttca tagccgtgtt tttctgatac
    14821 acatcatctt tatattgatt ttcagaaaat aaataatgaa agagcgttgc ttctttacga
    14881 acaacttcag ccattacatt aggaagcaat gcggatgcgg tacgttgtcc aaggacaaat
    14941 aaacctatga ttgcaatcac aatacccatt gcaacgtcaa taactcttgg aaacgcaatg
    15001 ttaattgtta aattctgtga tgctaatccg ttcattaaaa taacttgtat tgtaataaaa
    15061 actactgcaa tcgcataatt tgctcccacc aatgcttcag taaacaatgc tgcaatgccc
    15121 attaaaatta ctgcaacagg tgttggtatt gaaaacaaca atatgactga aagtactaac
    15181 acacctaaaa tagtacctaa acctcgtgcc atacctctct cgattgcatg tatagttgat
    15241 gttcctagta atattgtatg tgcagataac ggtatccagt atgctttttc aaaatcaaac
    15301 attaacgcaa taaatatcgc tatcatcata atcgctgtat atctcaatgt atttctaaaa
    15361 acaatagagt ctaatgttaa attttgataa atgcgtttac tatataaagg ttttcgcatg
    15421 tctgcttcat acgcaatacg cgcttcattt gcatgcacca tttcatcaat tttcaatata
    15481 tggttgaata aattttgaaa ttcctctgtt actgtcactt cttttcgcca atatcttaca
    15541 ttttcctcac tattatctag ttgtgcaatg atatgatcca tcatttcaat taactcatct
    15601 ggcaatggtc gaatttgttt agcgttcaac tctaacagtt cagaataaat cccttgggca
    15661 gatgtgtgta ataataataa tttttggaaa cgtctactta atttatcgtt acttgaagta
    15721 gaagtgatta ataatttatc agatgcttta aaactatcca ccgctgtttt tgctacttgt
    15781 aaaaaagcag atttatcatt ataagtgtgt aacaacttag atatgagttt aaaatcattt
    15841 tgaattgctt gttcttcagc tttgttttta gaaaatacga tcgtgattaa caccatcata
    15901 gtggcaagta taccgcctac taaaatcgca aagcctctat ataatgcttc ttcgggagct
    15961 ataggtaagt taatgggtag actgaatgtc acaatgaaga atgtcgatga cggtccagct
    16021 atttttaagg cagtaaatat ataaaatggt ataaccgtaa caattaataa tgtcattcca
    16081 aaaacgagtg gcgttttggc tgttagcgta ccaagcatca tacatattgc aaacgctaaa
    16141 ttacaaatta ttacagttcg cagcttagat cgcgacggac ctttaaaaac ataaatatgg
    16201 gctagtgttc cggttgcaac taataatcca aattggaaat taccacataa gtaaccgatt
    16261 attgcaggaa taatcattaa cagtccctgt cttacacctt tataaaagtc taagttaagt
    16321 cgattgattt ttatgagtga tgtcaaaaag atgtcatttc gacttctcct ttccttgtca
    16381 aaatatttcc ataatctttt tattagcata gttaaaatga atttgttgct ataaatttta
    16441 attacgcaac atatgtcatt ataacacctt atgctcaatt atctaaattt aaaaactttt
    16501 aaagtccaat taaaattaat ttctaatgtt atttaacatc gttgaacctt tggtcgccca
    16561 cctttgcgct agttaaaatt tatatgtact tttacaagcg ttacctgact aatgttttaa
    16621 aaccttattt ctttacatat atcatttcaa taacttcatt ttcatcttaa accatatctg
    16681 ttttaaaaag tttcaatttc aacgcaaaaa aagcaataag ccccaactat gtgaaagctt
    16741 actgcttgtt taaattattt ttctattgct ttatcatcac taacaattaa aggttgatct
    16801 ggtttcacat agaaccacat aatcacagcg attactgctg gaacgattaa caattggaat
    16861 gtcattgtcc aacccaaatg ttctacgaat agacctgcta atagcggact gattaatgca
    16921 cctatattac cccataagtt catccaccct gatactgtac cagagaagtt acgtcctaag
    16981 tcagttgctg cagcccaact catacccatt gaaataccga taccaccaag acctagtgat
    17041 aaccagaaaa tacttacata taaatttcct gtgcgtacag caaagataat tgaaactgca
    17101 aacacaataa atcctgcaat tgcaattaca cctctagcta cgaattttga acgacctaat
    17161 cctaatacac ggtcagaaat tgcacctgct gataagatta agaagaacat taataaccaa
    17221 ggtaatgaac taatgctcat ttctttaaag ttaacgtgga atacttctgt taaatacgtc
    17281 ggtaaccaaa ttaagaataa tgtaatgata aattgtacaa caaagtattg aattgcaatt
    17341 gcatagaagc taaaacgttt aaagaaatca ttccatggtg gtgatgactt ttcagtagct
    17401 acgatatcac gattttccat aatgaaacgt ttctccgctt cattaaccat tctatgttgc
    17461 tcaggtaagt ctttggcaat aatcgcccat aatacagcca ttaaaatacc tactgcacca
    17521 aaaatgtaaa atactgcttg ccagttaaat gcgttaacaa tagcaattgt aactattggt
    17581 gctaatacag gtccgaaata tgatcctgct aataatgcac ttgatgctct acctttttca
    17641 tttttagaga accaaaatga attaaacaca gcattagaag ggtacattgg cgcctcacct
    17701 acaccaaata agaatctcac taaataaatt aaaccgtggt tcttaatcat acccgttaag
    17761 attgtaaatg cactccacca aactaatgca attgtaatca tcttacgtgg accgaatttt
    17821 tcagccaaca tccccgatgg tacttgcatt aatgcataac ctagagagaa gaatgatgcc
    17881 aataaaccaa attgtggttt tgtcatccct aaatcatcca tcatttgctt agcgatatat
    17941 gaaatgtttg ctctatccat atacgcaata accccaataa agaagaacgc aagcgcgaac
    18001 atccacctta cgttagttcg tttttccttc ataactattc ctacttccct aattaatttt
    18061 ttaaaactta ccctgaaacg ttaaaatttt cggacatctg gcatgtccac ccctactttg
    18121 tcacaaaatt gtaccaaagt tattttttat aatattatca gacatggaaa gcgtttacaa
    18181 tagaatctat aaaaatttta ttaaattccg cttaactttt gctattaaaa cgatataaag
    18241 gtacttttag ttacatagta gcctatcgtt tgttaattaa aaatactgca atcctagtgt
    18301 tttaaaacgt atattgcagg ctttcttaca tattcgacat tcattttaaa ttgcatataa
    18361 ctacattaac cattcattgt gcagcgcctg ttgtttagat gatcatttat tgactttttg
    18421 ctcttatagc aagcaatttt ttgatttatt gcttataata cattttaaga caaatgtttg
    18481 tttcaacatc gcatgcgaca tcattaatta ttcatttgaa tttatccagt ttctatttgt
    18541 ctgcaattgc tgaaaattaa aggagcttat ttttaataat ggaacaaatt atcactgaat
    18601 ttattagccg ttttgggtat gcagccattt ttatattaat tttattagaa aatgtactac
    18661 ctatcgtacc atcggaaatt attctgacat ttgctggtct tatgtctgtt aaatcacatt
    18721 tatcaatttt aactttattt attattgcaa ctatcgcatc gtttataggg ctgttaattt
    18781 tatattatat ctgccgtttg atttcagaag aacgtctata tcgttttatt gatcgacacg
    18841 gtaagtggat taaattgaaa agtaaggatt taaagcgagc aaatgattgg tttaaaaagt
    18901 atggcgtatg ggctgtattt atctgtcgtt tcatacctgt attacgtgta ttgattacca
    18961 ttccagctgg tgtaaaccgc atgaatgttg tgacatttac cgttatttca ttaataggta
    19021 ctacaatctg gaatttcggt ttaattttac taggacgcac tttgagcgat agctttggta
    19081 tgttgatgac tggtcttcat acatattcac gtattatgta tgttgtcatc attatcgcag
    19141 tcatttactt tgctatacgt tacattggca aacgtaagag agtaaaataa tattgttgtt
    19201 agcataaagg catgaatttt aaatttctat aaacaaaaag cagtgaatca ttccatctat
    19261 tgaaatgatt cactgctttt ttaagtttat ttggcatact ttagctgaaa gtcaactatc
    19321 tttatataaa gaaatattac tattaacatg atagttatag ttgtgaagta aaaggctacc
    19381 aacggctgag aaatgatacc aattaataat gcaccgacag tcattccaat atatctcaca
    19441 aaattaaata cacctagcac tttccctttt atatgttcaa accttttaga aattatagta
    19501 gacagcaaag ctggcacaat accaacacaa gtacctaata taaatataat tactgataat
    19561 ataattacat ttgattcgtt taaccatcca aacaataacg taaatatagc aaatccagtt
    19621 atagtaacac gaagtatcat tatttcatta tatttttttg aaaatcgctt ataaaataaa
    19681 cttcctaata taacactaac agtaataggt aaaaacaaaa ctcctga
//
LOCUS       DAMQYT010000024        19594 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMQYT010000024 DAMQYT010000000
VERSION     DAMQYT010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19594
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.024"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(122..2098)
                     /gene="sfaD"
                     /locus_tag="PGA33_000732"
                     /gene_synonym="sfnaD"
     CDS             complement(122..2098)
                     /gene="sfaD"
                     /locus_tag="PGA33_000732"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HDG6472565.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPK
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPLIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSDFESSAKSWVETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            2200..3393
                     /gene="sfaA"
                     /locus_tag="PGA33_000733"
     CDS             2200..3393
                     /gene="sfaA"
                     /locus_tag="PGA33_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HDG6472566.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKIPEDASRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNM
                     YYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            3380..5137
                     /gene="sfaB"
                     /locus_tag="PGA33_000734"
     CDS             3380..5137
                     /gene="sfaB"
                     /locus_tag="PGA33_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HDG6472567.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            5144..6214
                     /gene="sfaC"
                     /locus_tag="PGA33_000735"
     CDS             5144..6214
                     /gene="sfaC"
                     /locus_tag="PGA33_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HDG6472568.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNHY"
     gene            complement(6315..6440)
                     /locus_tag="PGA33_000736"
     CDS             complement(6315..6440)
                     /locus_tag="PGA33_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472569.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
     gene            6606..7589
                     /locus_tag="PGA33_000737"
     CDS             6606..7589
                     /locus_tag="PGA33_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDG6472570.1"
                     /translation="MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            7601..8632
                     /locus_tag="PGA33_000738"
     CDS             7601..8632
                     /locus_tag="PGA33_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDG6472571.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIIHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFFIVFVTIIIPALEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWIIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            8629..9597
                     /locus_tag="PGA33_000739"
     CDS             8629..9597
                     /locus_tag="PGA33_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDG6472572.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MTFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            9833..11203
                     /locus_tag="PGA33_000740"
     CDS             9833..11203
                     /locus_tag="PGA33_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDG6472573.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(11335..11595)
                     /locus_tag="PGA33_000741"
     CDS             complement(11335..11595)
                     /locus_tag="PGA33_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472574.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            11772..12287
                     /locus_tag="PGA33_000742"
     CDS             11772..12287
                     /locus_tag="PGA33_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDG6472575.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPKGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            12331..13518
                     /locus_tag="PGA33_000743"
     CDS             12331..13518
                     /locus_tag="PGA33_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HDG6472576.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVRVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            13537..14220
                     /locus_tag="PGA33_000744"
     CDS             13537..14220
                     /locus_tag="PGA33_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDG6472577.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            14397..15740
                     /locus_tag="PGA33_000745"
                     /pseudo
     CDS             14397..15740
                     /locus_tag="PGA33_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            15839..16312
                     /gene="sepA"
                     /locus_tag="PGA33_000746"
     CDS             15839..16312
                     /gene="sepA"
                     /locus_tag="PGA33_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HDG6472578.1"
                     /translation="MIVNYLKHKFYNLLTTMIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            16634..18076
                     /gene="lmrS"
                     /locus_tag="PGA33_000747"
     CDS             16634..18076
                     /gene="lmrS"
                     /locus_tag="PGA33_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HDG6472579.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTATGLI
                     IDQASWRVPFIIILGIAILAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALILSMVIIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            18224..19288
                     /locus_tag="PGA33_000748"
     CDS             18224..19288
                     /locus_tag="PGA33_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HDG6472580.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            19377..19562
                     /locus_tag="PGA33_000749"
     CDS             19377..19562
                     /locus_tag="PGA33_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472581.1"
                     /translation="MLSNKRVEKEAGCEHKKFLKKLLTLNSKVDNMIKSSKTTQLFLK
                     KNFKSVKLTIEITIKTI"
BASE COUNT         6474 a         2666 c         3597 g         6857 t
ORIGIN      
        1 cctgtttttt gtgattttta ataatttgaa tatggaaaat gtattattct ctcatttgta
       61 tagattgtat ttaataagtt aatgtaatcc ttgagctcac gattaataaa attctataac
      121 cttaattatt ttctcgatac aaagggttat taactttaat ataagtataa tgatgtgcct
      181 catcttcaag acgcattgtt gtaacacact tataatctat aaatggcgcg aacatggtat
      241 ctttaatttc atttatgcga ttctcattta cttgattaga tttgtgtgtc gaaagtacaa
      301 gttgatcaaa aatgttatct agtacatcac gaacgatata ccacatatgt ctttctaagt
      361 ttgaatcatt tgatgcttta gagattgtaa gaattaattc gcctaaatgg ttttgaacgg
      421 tagaataaaa ggctttgtta aacacagacg tttttgaatc agtaagaatt cttgattttt
      481 catggaaatg agatgtactg tatcccattt catttagttg tgctttatca attcttaaac
      541 cttcaaagtc tctaatatac atcgtgtcca atagaccatc tttccgaaat gttgcaatag
      601 cattttgcaa gtgagcctct aatgcaatgc catatttagt aactagtggg attacgagac
      661 caagcaatgc tttactataa gtttcaaccc acgatttcgc cgatgattca aaatcagaca
      721 atgatgcagc tgattgataa cgtttaatca atgtcacgat aggtgattca ttgttaaatg
      781 ggtaggttgc aactaagctt gaaggaatca atggtgtgac ttcttgtgga atcatttggt
      841 atatattttt tctaaataat gtacctaatt gttcacttcg ttcagtttga tagtctgctt
      901 cgtcttgctc attataaaaa tgaataccag caacttcatc aataattgtt gatgcatagg
      961 acttaaatat gacatctttc tccaaaatat catttaaaat acgtgtcatt agtggaccat
     1021 tgtgcgtcgt ttgttctgat aatgtacgaa tctcacctgt aatatgaacg tttgtcgaca
     1081 atttgatgtg tggcgacata gctgggtatt taggaactaa tgttctgaaa gataaaccag
     1141 cataataatc caacgtatgt tttgcttcaa tgattaattc tttatctact tctgcttgat
     1201 aatcagaatg taatacgtca tctaattgcc atggatgaac aatcataatg tgataatcat
     1261 taaagttaaa ctttggcgta aattcatttt ctaattgttt aattaagtcc ggaaatagtt
     1321 gatgaacagt tgtatcataa tctttagaca gtgacatagt acggcttaat ttactgtgaa
     1381 tcagtactat tttcaactta ataggttgat tgaattctga agaatatagg aatgtttgta
     1441 atgcatttaa acctttacgt aatttagccc caggatgtag cggatgacct tcaataacgg
     1501 cttgctctga acgcaagtaa ctatcttcgc tattttcgat aatattaaat aaaggtgcag
     1561 aatcatgttg cattgacagt gcttgatagc taattgcaaa tgtcatatta gttgcactgt
     1621 ttattaaatc ttgctgaaat tgatcactag cagcattttt taaatctggt gcttcaatta
     1681 aaatacactc aagaatttca tttggatggt gtactcgtgt aatcgtattt gtaatgtcat
     1741 ctttaatgta gaaagggcct tcaacatcaa ttcgatcaaa ggcgtgttct ccagtgatag
     1801 gagcatataa tgtttgctta gcttgtggga actggatttc tagtatatga gtcgttgaga
     1861 tatctaacat aatcaaatca cgactcaata ttttcttact ttgagtgcgc gctttaacta
     1921 agttttcgcg atgcattgat gtgaccaatc tctgagtgac tttatctctt ccttgtaaaa
     1981 tcatctcttt aaaaatatta gcccaatcgc tattatgttg ttgtaaaaat aaatatgttt
     2041 cttgttcttc tttgttaaat tttaatgtct gttctttaaa aattaagttc aagttcataa
     2101 ttcacctcta tgaaatattt tacaaaagca agatagattt gtataatcca tattaatgat
     2161 aatgattctt attatcaaca gaatgcgggt gtaagtttta tgacaaaata tttttttagc
     2221 agttcttttc tactatttct aggtaattgg attggacaaa tagggctaaa ttggtttgta
     2281 cttaccactt atcataacgc agtttatctg gggattgtca atttttgcag acttgtacca
     2341 atattattac taagtgtgtg ggcaggggca attgccgata aatatgataa agggcgattg
     2401 ctgagaatta caatttcatc atcattttta gtaactgcaa ttttatgtgt gctcacgtat
     2461 agtttcactg caattccaat tagcgtcatt attatatatg cgacattaag agggatttta
     2521 agtgcggttg aaacaccttt aagacaagca atcttaccag atttatcaga taaaatatct
     2581 actacacaag ctgtatcatt tcattcattc atcattaata tttgtcgttc aatagggcct
     2641 gccattgctg gtgtcatatt agcagtctat catgcgccaa caacatttct tgcacaggca
     2701 atttgttatt ttatcgcagt tttattatgc ttaccattac attttaaagt aactaaaata
     2761 cctgaagatg catcaagata catgccgtta aaagttatta tagattactt caaattacat
     2821 atggaaggtc gacaaatatt tataacatca ttattgatta tggcgacagg tttttcatat
     2881 acgacacttt taccagtttt gacaaacaaa gtatttccgg ggaaatctga aatatttggt
     2941 atcgctatga cgatgtgtgc cattggtggt attattgcaa cgctagtttt acctaaagta
     3001 cttaaatata ttggtatggt aaatatgtat tatttaagtt catttttatt tggcattgct
     3061 ttgttaggtg tggtatttca caatattgtc atcatgttca tttgtattac attgattggg
     3121 ttatttagtc aatgggcacg tacgacaaat cgcgtttatt ttcaaaataa tgttaaagat
     3181 tatgaacgtg gtaaagtact gagtattatt atgatggata gaggtatgat tccattggga
     3241 agtctattaa tgagtatatg tgcagatgtg tttggcattg ttagaacttt ttcaataatg
     3301 ggaataagta ctatatgcat tacaatggta ttctatttta taaatagaaa gttgaagtta
     3361 aagttggagg aaagtaatca tggtatatct tgaatgggca aaggcagata gaaatattca
     3421 atatcgtgta attaacgcca ttattaaaga acgtatttac cccgagcaaa catttatttc
     3481 gcaaaaagga tctttaattg aaattcagta tcatatgcat gtgttgacta ttgaagttgt
     3541 tagaaaaagt gcattagaac gctatgagtt tacaggtgat attacttatt taaataaagg
     3601 tgaaacgtca ttaattataa ctttagaagg tttattagat gtgttgaatc atgactttga
     3661 tatccctatt tcagagcgac tacgcgaaga gttaatacac agtcgagata gtttagttga
     3721 aacatataag caaatgtctc acagacaaac gttaataagt caaagtttta aattttcaag
     3781 gttaccacaa gatattaact ttttttcatg gttacaacat gtaaaagata gtgataagac
     3841 agatgattta acttattctg agagtttggt accagagggg catccaacac accctttaac
     3901 caaaacgaaa ttgcccttaa ctatggaaga agtacgagca tatgcacctg agtttgaaaa
     3961 agaaatccct ttgcaaatta tgatgattga aaaagaccat gttgtgtgca cagctatgga
     4021 tggtaatgat caatttatta ttgatgaaat aattcccgaa tactacaatc agattcgtgt
     4081 gtttttaaag agtttaggtt tgaaaagtga agactataga gcgattttag tacatccttg
     4141 gcaatatgat catacgatag ggaaatattt tgaagcatgg attgctaaaa aaatattaat
     4201 tccaacgccg tttacaatac tttcaaaagc aactttatca tttaggacga tgtctttaat
     4261 tgataaacca taccatgtta agttgcccgt cgatgcacaa gcaacaagtg ccgttagaac
     4321 agtctcaact gtgactactg tagatggacc aaagttaagt tatgctttac aaaacatgtt
     4381 gaatcaatat ccaggattta aagttgctat ggaaccgttc ggtgaatatg caaatgttga
     4441 taaagatagg gcacgtcagc ttgcttgtat tataagacaa aagcctgaaa ttgatggaaa
     4501 aggtgcaaca gtagttagtg caagtctagt taataaaaat ccaatagatc aaaaagttat
     4561 cgtggatagt tacttagagt ggttaaatca aggaattact aaagaaagta ttacgacatt
     4621 tattgaacga tacgctcaag cattaatccc gcctttaatt gcttttattc aaaattatgg
     4681 aattgcttta gaagcacaca tgcaaaatac agtagtgaac ttggggccac attttgacat
     4741 tcaattttta gtgagagatt taggtggttc tagaattgat ttagaaacat tacaacatcg
     4801 tgtatcagat attaaaatta caaatgatag tttaatagct gattctatag atgcagtgat
     4861 tgcaaaattc caacatgctg ttattcaaaa tcaaatggca gaattaatcc atcattttaa
     4921 tcagtatgat tgtgttgaag aaaccgaatt atttaacata gtacagcaag tagtagcgca
     4981 tgccattaac ccaacactac cacatgcaaa tgagttaaaa gatattttgt ttggaccaac
     5041 aattactgtc aaagcgttgt taaatatgag aatggaaaat aaagtaaagc aatatttaaa
     5101 tattgagtta gataatccga taaaaaaaga ggtgtagtac tacatggcac acgttaacat
     5161 aaatatatcg aagattaaat ataacgccaa agtacttcaa acagtttttc aaagtaaaaa
     5221 tatgcaattc acaccagtaa ttaagtgcat agctggtgac cgtacaattg tagaaagctt
     5281 aaaagcgtta ggtatcaatc atgttgcaga atccagattg gataacataa ttagtattgc
     5341 agatcaggat ttaacatata cgttattgcg tacgcctgcc aaaaaagaga tttcagatat
     5401 gatagaaaaa gttgatatga gtatccaaac agaattatct accatacatc aaataaatga
     5461 agtagcagaa gttttaggaa aaaaacataa gattttatta atggtagatt ggaaagatgg
     5521 ccgtgaaggt gttctgactt atgatgtttt agattatatt aaagagatta ttcacttgaa
     5581 aaatatccat tttgtaggtt tagcattcaa ttttatgtgt tttaaatctg atgcgccaag
     5641 tgatgatgat atctttatga ttaatcgatt cgtctcagct gttgaaagag aaataggata
     5701 tcgattgaaa attatttcag gtggtaattc gagcatgtta ccacaacttt tatataatga
     5761 tttgggtaaa ataaatgaat tgcgaatagg tgaaacgttg tttagaggtg ttgatacaac
     5821 gacaaatcaa gcgatcgcta tgttatatca agatgcgatt acattggagg ctgagatttt
     5881 agaaattaaa cctcgagtta atacacaaac gcatgaatca ttcttacaag ctattgtaga
     5941 tattggttat ttagatacaa aagtcgataa tatttcacct atggatcagc atatcaatat
     6001 tttaggcgct tcgagtgatc atttaatgct tgatttaaat ggtcaaggac actatcaagt
     6061 tggagatcat atatcttttt cattaaatta tgaagcactt tctcattcga tgtatatgaa
     6121 aaatttacat aaggtatata ttgatgattc aaagattgat acgctacttc aaaattttga
     6181 tgtgaaatct cctgctatgg taaaccacta ttaatatatt tatcaataag tctaagttga
     6241 caagttatac tacatatcgt ttgcacggtt gtatcataat tgttcaactt agattttttg
     6301 tatttgttga tttatcaaat taagtgcaac agttcgtcac ataaaattgc aacagataat
     6361 atcagctgaa ttacagggat aacggtcatg ctaaatggtg tcaattgtat taatgcaaag
     6421 atgatatagc aatgatacat tatcagtatt ttgtctaagg aaatgtgcta attgtagtca
     6481 taattattag aggaaatata tagtcataca ttttagaaat acaaaaaaga ttgaacgcta
     6541 cttgacaatg ataattgtta tcaataaaat aataaatgaa gttatacata ttaaggagtg
     6601 gaacgatgag aggtctaaaa acttttagta tattgggatt aatagttgcc ttacttttag
     6661 ttgcagcttg tggtaatacg gataattcaa gtaaaaaaga atcatcaact aaagatacta
     6721 tttcggtaaa agatgaaaat ggtacagtaa aagtacctaa agatgcaaaa cgtatcgttg
     6781 tattagagta ctcatttgca gatgcattag cagcattaga cgttaaacca gttggtattg
     6841 ctgatgatgg taagaaaaaa cgtatcatta aaccagttag agaaaaaatt ggggattata
     6901 cttctgtagg tacacgtaaa cagccaaact tagaagaaat tagtaaatta aaaccggatt
     6961 taattatcgc tgatagcagt agacataaag gtattaataa agaattaaac aaaattgcac
     7021 caacattatc attaaagagt tttgatggag actacaaaca aaatattaat tcgttcaaaa
     7081 caattgctaa agctttaaat aaagaaaaag aaggcgaaaa acgtcttgct gagcatgata
     7141 aattaatcaa taagtataaa gatgaaatta aatttgatag aaatcaaaaa gtgcttccag
     7201 cagtagttgc taaagctggt ttattagcac atccaaacta ttcatatgtt ggacaatttt
     7261 taaacgaact aggatttaaa aatgcattaa gtgacgatgt aacaaaaggt ttaagtaaat
     7321 atttgaaagg accttactta caattagaca ctgaacattt agctgattta aatccagagc
     7381 gtatgatcat tatgacagat catgctaaaa aagattctgc tgaattcaag aagttacaag
     7441 aagatgcaac atggaaaaag ttgaatgcag ttaaaaataa tcgcgtggat attgttgacc
     7501 gtgatgtttg ggcaagatct cgtggcttaa tttcttctga agaaatggct aaagaacttg
     7561 ttgaattatc aaaaaaagaa caaaagtaag gtggaagtaa atggctataa aagaaataag
     7621 tagccaatct gccatagatc ataaaagaaa aagacgcaca acactcacgt atatagtgag
     7681 tttgtgcttt ctttttattt gtatatattt aaatatggcg attggttctt cgaaaattaa
     7741 ttttagcgat atcattcact atgttactgg tcatacagat acgaaagcaa cgtttttatt
     7801 gcataatgta cgtatgccaa ggatgattgc agggttattt attggcggtg cattagcggt
     7861 atctggtttg ttaatgcaag caatgacacg caatccgcta gcatcaccta aaatttttgg
     7921 tgtcagttct ggtgcatcgt ttttcattgt atttgtgacg attatcattc cagcattaga
     7981 atattatgca ttatatttag gtgtgatagg tgcatttata ggtggtttaa ctgtttatac
     8041 actttcaggt gctacgaaag gtatgacacc cattaaatta gcattggctg gtatggccat
     8101 ccatttgttt tttagtagta tgaccgaagg tattattatt ttgaatgaga attcaaacga
     8161 acaagtgatg ttttggcttg tcggttcatt atccagtatg aagtgggatg aaattttaac
     8221 cattctacca tggattattg gtgctttaat tgtaacgatt tttattggac gtcaactaac
     8281 gataatggaa ctaggcgatg acattgctaa gggattgggt caaaatataa ataaagttag
     8341 aattatcatt ggactattag tcattattct tacaggtatg tcggtgtcag tggcaggacc
     8401 aattggattt gttggcttaa tcgtaccgca tatagtcaaa cgctatgtga gtaagaatta
     8461 tttagtcatg attccattaa catttattat aggtgcagat ttgttactac tatcagatgt
     8521 attaagtcgt ttaattacat atccttatga atcaccagta gggattgtta catcattcgt
     8581 aggtgcactt tacttcttat ttataacgat taaaggggtg aaaagaatat gatgacaacg
     8641 aaaattatac gtcgttatag tttaatcaca atccttttaa ttgtgagtat attcataagt
     8701 ttatgtgtag ggtctgtcat gattcatccg attgatgcta ttaagggaat ctttacacaa
     8761 gatgatttta ttttaaatga gtatcgtata cctagaacat tacttggtat tattatcggt
     8821 agtagcttgg ctatttcagg tgcaattata caagctgtaa taagaaaccc gttagcctca
     8881 cctgatgtta taggtatttc taaaggtgcc agtttagctg ctgtgattat tattatgacc
     8941 tttccaacag caccattgtt tgtattacct ataggttcat tcttaggtgc ctttgcagta
     9001 agtttatttt tatcattttt aatttcaaaa tatgatgtta aaggttcaaa gcttgcctta
     9061 attggtttag caataggcgc aatatgtacg gcgattgtgc agttcttatt aatcagaaat
     9121 ccactggaag caaacaaagc gcttgtttgg ttgacaggta gtctatatgg acatgatatg
     9181 tcgaatattt atactatatt accttggttt ttaataacga taccaatcat cattttattg
     9241 agttatcaat tagatgtatt gaatctaggt gatgctgttg caaccgcact cggtttaaaa
     9301 gttaaaacta ttaaaatgct attgttaatt ttagcagtaa tattagcagg atccgcaatt
     9361 tcagttgtcg gtggtattag ctttttagga ttaattgcac ctcatattgc gagacaatta
     9421 ataggaaata aaacattaca cgttattatc atgtcggggt taattggtgc tatattgtta
     9481 acatttggag atggtttagc ccgtggcatt catccgccac tggatattcc tgttggggtt
     9541 atcattgcaa ttatcggtgc accatacttc ttaatattat tacgaagaat gaaataacta
     9601 acatatgatt agagtttaaa aaaggttaag tacgaaatca gtaattgtac ttaacctttt
     9661 ttgaattcat cttgaaaaat tacaagaaat agcgtatatt taataactgt aattattcat
     9721 tattttgcat acataaacat tttgaaagat agaatgataa ggaatatgtt tgttaactga
     9781 atagaattct gtatatactt aaaacgaata catagagaaa tggattgatt ttatggatag
     9841 cttttcaagg aaagagatag ttattgggag acttaagttt attacgatgt ctttaattgg
     9901 tatcttatta tttttagtac caattccagt cgaacaagat ggacagaagc aaacaacgtt
     9961 gcccgtggca tttttagcag gcgtgcttaa agatgtatta ggtggcgtaa tgccattttt
    10021 aattgtgacg ataattactt tatcgggtat cataactctg atttgttcaa ctattttaaa
    10081 agataaattg aaacctgacg ggctaatgaa caatgctttt aacgttagaa ttggttggtt
    10141 aattttacga atattagcag ttgtttttgc atggatgaca tttttacgaa ttggttcaaa
    10201 ggttatttac tcagatgaaa cgggtggatt attgttctca agtttattgc caacattagt
    10261 ggcagtattc ttatttgcag ctctattttt accgctatta atggaatacg gtttgttaga
    10321 gatgttgggt cctattttta gaccggttat gagaccttta tttacattgc caggacgttc
    10381 gacggtagat aatttagcat catttattgg agacggaact gttggtgtct taattacaag
    10441 tagacaatat ggtgaaggat attattctcg tcgtgaggca acagttattt caacgacatt
    10501 tagtgtagtt tctatcacct ttgcaattgt agttgctgaa acagtgcaca tgcaaaatca
    10561 attttttgct ttctatttat cagtgatagt atcctgcctc gttgcagcag tcattatgcc
    10621 aagaatttgg cctttaaata aaattcctga tgagtatgct aaggaggtac ctgaaagtgc
    10681 gcgtactgag gcattgccag aaggaaagac ggcattgaga catggctttg atactgcaac
    10741 agaagtaggt attaaagcac cgggcgttat tgactttttt aagtcaggat taaaaacagt
    10801 cattgatatg tggtttgtta ttttgcctgt agtaatgagt attggtacta ttgctaccat
    10861 tattgcgaac tacacgccct tttttgtcat attgggtaaa ccttttgttc catttttaga
    10921 gttaatgcaa atacctgaag cggcacaggc ttctcaaacg attattatcg gttttgccga
    10981 tatgttttta ccttctatat taattgaagg tgtacaaaat gatataactc gttttgttat
    11041 tggggcactt agtatatcgc agttaatata cttgtctgaa gtaggtggcg tcatacttgg
    11101 ttcgaagatt cctgtaagca tcggcaaatt gtttatgatt ttcttgatta gaacaatcat
    11161 tacactacca attatttctt tgatggcaca tcttttgtta taaatatatg tgaaaaagga
    11221 caactgttaa aattatggga acagttgtcc ttttgtattt gtttatgctg ggtgttgaat
    11281 caattaaaaa aatcattcat gaagccctca ccatgaatga tttttttata caatttattt
    11341 agttaattca ttgtaaactt tttgatcatt ttgagtataa ataatgtatt cgtggtcttc
    11401 agtatcgata ataacgcgat tcgttttacc aaacgttgag ccgattctag atacatcttt
    11461 gctgtctaat ttaggaactg catgaatatc ttggtcttgt gtaatatttt taatttcact
    11521 tgtaggaatt ttgatatcag caactctcca ttgaatacgc acttcattat tatccttttt
    11581 tactgtcatt gccattattc aaacacatcc tttaaatatt ttgtaaatac atcataaacg
    11641 attttgtaat cgttttcaag agttatgtga tactttttta aattgtgtca tactaatgaa
    11701 ttttaacccg aaaatatgtt cgtgtttttg ttagggatgt ggtaaaatgt gagtattaaa
    11761 ggagaacaaa tatgacaggt aaaacacatg cttcatgcgg catgctcgtt ggagcgctaa
    11821 caacacaata ttttcaaacg gatatttttt cgtcggttac ggttatcatt ctagcaactc
    11881 tagctagctt attacccgat atatgtcaca ctcaaagtaa gattggcaga aggtttaagg
    11941 taattagttt ttttgtgaga ttgatctttg gacatcgaac ctttacgcat tcaatattat
    12001 ttatcgcaat catagcattt ttattgcaaa ttattcagac gcctaattat tatatggcag
    12061 caattataat tggattagtg tcacatgtta tactagatat gataacacca aaaggcgtga
    12121 aattatttta tccgttaccg tttaatgtga agacaccgat tcaatttaaa acaggtggac
    12181 ttgtagactt atctttagct acagcgttaa tggtcggaac agtttatgtt ttatttcagc
    12241 ctttcatcaa tgatataata agcaactgga atactaagtt tttatagaaa ataaattatt
    12301 aaaggttaca aactcagcag gaggcataaa atgctagata aaaatcaatt agctaaatat
    12361 aaacaagatc atttgtgtga atatgaaaaa ataatgagta acaatgaaaa agaagcactt
    12421 gaagagaagg tagcgtcatt agatttagat tttattgcca aattatataa cgatttatac
    12481 atcaataaga aaacaataga tgatgtatct gcagtttctg aagtgaaata tgatattaaa
    12541 tcacaaatga gtgatgatga gattaagcgt ttagaggaac aaggacttca agctattaaa
    12601 gaaggacaat ttgcagtact tttgatggca ggtggtcaag gaacaagact tggttacaag
    12661 ggccctaaag gttcgtttga aattgaaggt gtaagtttat ttgaacttca agctaaccaa
    12721 ttaaaaacat tgaatcatca atctggacat acgattcaat ggtatattat gacaagtgat
    12781 atcaatcatg aagaaacttt agcttatttc gaagcacata gttattttgg atatgatcaa
    12841 gaagcaatcc atttctttaa acaagataat attgtggcgc ttagtgaaga agggaagtta
    12901 attttaaatc aacagggtcg tattatggaa acgccgaatg gtaatggtgg cgtgtttaaa
    12961 tctttagaca aggcaggata cttagaagaa atgtctaata atggcgttaa gtatattttc
    13021 ttgaataata tcgacaatgt tttagtaaga gtgttggatc cactatttgc aggatttacg
    13081 gtagaacatg attacgatat tacttctaaa acgattcaac caaaaccagg agaaagtgtt
    13141 ggacgtttag ttaatgtcga ttgtaaagat acagtactgg agtattcaga attagatcca
    13201 gaagttgcaa atcaatttaa caatgctaat atcggtattc atgcatttaa acttggtttt
    13261 attttaaacg ctgtaaatcg tgagttacct tatcatttag ctattaagaa tttgaagcaa
    13321 ttagatgaga actttggtgt tattgaacaa ccaactttaa aattcgaatt gttctatttt
    13381 gacatattta cttatggaac tagttttgtc acgttacaag ttcctagaga agaagaattt
    13441 tcacctctta aaaataaaga aggtaaagat agtgttgcaa ctgcaacaga agatttacgt
    13501 agaatgggtt taatttaatg ataggcggtg actatgatgt ccaaatcaac taaaaaaagt
    13561 aatgacacaa tcgtagaaac atttaaggat atcatccctt tatcatttgg agaagaaatt
    13621 ggtaatgcgg catctcatgg tgttgcagct ttgttaacat tattagtgtt accctatgcg
    13681 gctgtgcaca gttatattgc acatggtacg cttgcgtctg tgagtatgtc tatttatgtt
    13741 atttctatct ttatgatgtt tatttcatct actatttatc attctatgca aaatgaaaca
    13801 cctcataaat atattttaag gattatcgat cacagtatga tttacgtagc aatttctggt
    13861 acatatacgc ctattttgtt aacagttgta ggtggttgga taggttggac agtatttatt
    13921 ttattatggg gaacgacact ttgggggatt ttatataaat ccatagctgt caaagttaac
    13981 caaaagttaa gcttaattgt ctatttagtg atgggctggg taggtattat cttgttacca
    14041 attattttta ttcgcacatc atggatattc atattattca ttttcttagg tggtgtgtct
    14101 tatacaattg gtgcatggtt ttatgcacaa aagaatagac catattttca tatgatttgg
    14161 catattttta ttgtaatagc atcactattg catttaattg caatcttata ttttatgtag
    14221 tcgtttagaa aagctggggg aaactccgct ttttttatta cataaatcat tctaataggg
    14281 gattgtattg taatagaatt atcgttaaga tttgaaagtc aaaagtgttt atacatatga
    14341 aaaccaatta tttgtaaata aaatcttata aataaatata tagaaggagg tatttcatgc
    14401 gattaaagtc aatcataact gttatagctt taatattaat tatgtttatg tcagcgattg
    14461 aatcatctat tatttcgctt gcattaccta caattaaaca agatttaaat gcaggaaatt
    14521 taatttcatt aattttcaca gcgtatttta tcgcattagt aattgccaat cctatagtgg
    14581 gcgaattact ttcgagattt aaaattattt atgtggcaat tgcaggatta cttttattta
    14641 gtataggtag ttttatgtgt ggtttaagta ctaattttac tatgctaatt atatcacgcg
    14701 tgatacaagg ttttggatca ggtgtattga tgtcattatc gcaaattgtt ccaaagttag
    14761 catttgaaat tccgttacga taaaaaatta tgggcatagt tggcagtgtt tggggtattt
    14821 cgagtatcat tgggccactt ctaggtggtg gtatattaga atttgcaaca tggcattggt
    14881 tattctacat aaatataccg attgcgatca ttgccattat attagttata tggacatttc
    14941 attttccaga ggaagaaaca gttgctaaat caaaatttga tactaaaggg ctcacgctat
    15001 tttatgtatt tattggattg attatgtttg ctttattaaa ccaacagctg ttacttttaa
    15061 atttcttgag ttttatctta gctatcgttg tggcaatgtg tttatttaaa gtagaaaaac
    15121 atgtttcttc accattttta cctgtggttg aatttaatcg ttcgattact ttagttttta
    15181 taactgacct tctaacagct atttgtttaa tgggattcaa tttatatatt ccagtctacc
    15241 ttcaagaaca actaggatta tctccattgc aaagtggatt ggttattttt cctttatctg
    15301 tagcttggat tacattgaat tttaatttac atcgaattga agcaaaacta tcaaggaaag
    15361 ttatttactt actatcattt acattgctac tagtaagtag tattatcatt tcatttggta
    15421 ttaaattgcc ggtacttata gcatttgtgt taattttggc aggattaagt tttggatata
    15481 tttatacgaa agatagtgtg attgtccaag aggaaactag cccattacaa atgaagaaaa
    15541 tgatgtcatt ttatggatta actaaaaatc ttggggcatc aataggttca acaataatgg
    15601 gatatcttta tgcgatacaa tcaggaatct ttggtccaaa cttacacaat gtgttaagtg
    15661 ctgttgctgt aattagcatt gggcttattg ttttatgggt cgtatttttt aaagaacagt
    15721 catctcaatc aaaagaatag aattatgatt aaaattgatg tagtgtaatt aaagtaagaa
    15781 ataataaatt tcctagatac tagataaatc gttcaatatt ttattaataa ggaggccaat
    15841 gattgtgaat tatcttaaac ataagtttta taacttgtta actacaatga ttgttctctt
    15901 tatttttgta ctttctggtg cgattttttt aacatttcta gggtttggtt tatatggatt
    15961 aagtagaata cttatttatt ttaggttggg tgactttaca tataatagaa gtatgtacga
    16021 taacctatta tattatggca gttatataat ctttgggtac ttcatcatat ttgcagtcga
    16081 gcatttaatg gattatttta gaaaaatgtt gcctgaaaat gcatatttta gaggcgcgac
    16141 ttttcatttg atttcttaca cagttgcaac aacgttattt tattttatta ttcatctgaa
    16201 ctatgtgtat atcaatatcg atttttgggt catcatggtt attatagggt tcttatatgt
    16261 ttgtaagctt caattttacc ctgaaagtaa aaatttaaat aatagaaaat agatatctga
    16321 agttgagggc acatatgtgt tccaacttct ttttgttcaa tagaagaatc gataatagtg
    16381 agtcttagtg ctcaatttgt ggtaaataag ttaaataacc attgtatgag tctgagacat
    16441 aaaataaatt attaggccct acaatgttat attgttagtt tttaagtaca cattagctgt
    16501 gcctaatgtg taagaaccaa taatgattta ttatgtagtg cttctttatt atttgagtac
    16561 tactcccatt atgttcatgg ctatacgtat taaagggaca agtgtatatc tgttttaaaa
    16621 aggaggaatt atgatggcta aagttgaatt aacaacccgg agacgaaact tcatagttgc
    16681 tgttatgttg attagtgcgt ttgtagctat tttaaatcaa acattattaa atacagcgtt
    16741 acctagtata atgagagaat taaatatcaa tgaaagtaca tcgcaatggc tagttactgg
    16801 gtttatgctt gttaatggcg tcatgatacc tctgacggca tatctaatgg atagaattaa
    16861 aactagacct ttatacttag cggcgatggg gacattttta ttaggttcta ttgttgcagc
    16921 cttagctccg aattttggag ttttaatgtt agctcgtgta attcaagcga tgggtgcagg
    16981 cgtacttatg cccttaatgc aatttacgtt atttacattg ttcagtaaag aacatcgagg
    17041 ttttgcaatg ggactagcag gtttagtaat tcaatttgca ccagcaatag gacctacagc
    17101 tacaggatta attattgatc aagcgagttg gcgagttcca tttattataa ttttaggaat
    17161 tgctatactt gcctttgttt tcggtttggt ttcaatctcg agttacaatg aagtgaaata
    17221 tacgaaatta gataagcgtt cagtaatgta ttcaactatt gggttcgggt taatgctata
    17281 cgcatttagt agtgcaggag atttaggatt tacaagtcca atagtaatag gtgcgttgat
    17341 attaagtatg gttattatct atttatttat acgtagacaa tttaatatta ctaatgcact
    17401 tttaaattta agggttttta aaaatagaac atttgcatta tgtacgatta gttcaatgat
    17461 tataatgatg tcaatggttg gacctgcgct gcttataccg ctatatgttc aaaacagttt
    17521 atctttatct gccttgttat caggacttgt tatcatgcct ggtgcaataa taaatggtat
    17581 tatgtcagtt tttacaggta aattttatga taagtatggt cctagaccat tgatttatac
    17641 tggttttaca attttaacaa ttactacaat tatgttgtgt ttcttgcata cagacacatc
    17701 ttatacgtat ttaatagtcg tatatgcaat tagaatgttt tcagtttctt tactcatgat
    17761 gccgataaat actacaggaa ttaattcttt gagaaatgaa gaaatctcac atggcacggc
    17821 tattatgaac tttggtcgtg taatggctgg ttcactaggc acagctttaa tggttacatt
    17881 aatgagtttt ggtgcaaaaa tatttttatc tacatcgcca tcgcatttaa ctgcaactga
    17941 aattaaacag caatccattg ctataggggt ggatatctca tttgcttttg tagctgtgct
    18001 tgttatggca gcttatgtga tagcactttt tataagagaa cctaaagaaa tagaaagtaa
    18061 tagaaggaaa ttttaaaata attatagtag ttggtctatt taaaataata ggctaactgc
    18121 tttttttatt taataaaaag ttttatactt ttagtgatag actaagcaaa aattgttatt
    18181 tgctatgatg tagatgtctt aaaatgatta agggggattt gctttgttaa cggtagatca
    18241 agtgaaagaa ttggtaggag aaattaaaga tcctattata gatgtgcctt taaaagaaac
    18301 agaaggtatt gttgaagttt ctattaagga agaaaaagaa catgtgagtg ttaaattagc
    18361 aatggcacaa ttaggtggtg caccgcaatt agatttacag atggctgttg ttaatgcatt
    18421 aaaagaaaac ggtgcgaaaa cggtcggtat acgatttgaa acattgccgg aagaaaaagt
    18481 aaatcaattt aaaccaaaag aagaaaataa acctaaaacg atagaaggcc tattatctca
    18541 aaataatcca gtcgaattta ttgcaatagc ctccggtaaa ggtggtgtcg gtaaatctac
    18601 tgttgcagta aatttagccg ttgccttagc tcgtgaaggg aaaaaagtcg gattagtaga
    18661 tgccgatata tatggattta gtgtaccaga tatgatgggt attgatgaaa agcctggaat
    18721 taaagggaag gaagtaattc cagttgaacg tcatggcgtt aaagttatat caatggcctt
    18781 ttttgtggaa gaaaatgcgc cagttatatg gagagggcca atgttaggta aaatgttgac
    18841 gaatttcttt acagaagtta aatggggaga cattgaatat ttaatactcg atcttccacc
    18901 tggaacagga gatgtagctt tagatgttca tacgatgtta ccttcaagta aggaaattat
    18961 tgtaacgaca cctcatccta cagcagcatt tgttgcagct cgcgcaggtg cgatggcaaa
    19021 acatacggat cattctattc ttggcgtaat tgaaaacatg agttattttg aaagtaaaga
    19081 gacgggtaat aaagaatatg tctttggcaa aggtggcggt actaagttag ctgatgaact
    19141 taatactcaa ttacttgggg aattaccttt agagcaacca tcttggaatc caaaagattt
    19201 tgcaccgtca atatatcaat cagatgatcg tctaggtaaa atttatagct cgattgcaca
    19261 aaaagttata gctgctacaa ataaataggt ataatatatg ctaaacgtac aaaattactt
    19321 ttattttgta cgtttttatg ttgatttgtt ttggaatgct ggtaaaataa gttcttgtgt
    19381 taagcaataa acgcgttgaa aaagaagcgg gttgtgaaca caaaaagttt ttaaaaaagt
    19441 tattgacttt aaattccaaa gttgataata tgataaagtc gtcaaaaacg acgcagttgt
    19501 ttttaaagaa gaatttcaaa agtgtaaaac ttaccattga aataactata aaaacaatat
    19561 agaataaata aagtaatcgt tcaaaaagtt attg
//
LOCUS       DAMQYT010000025        19140 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMQYT010000025 DAMQYT010000000
VERSION     DAMQYT010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19140
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.025"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(18..374)
                     /locus_tag="PGA33_000750"
     CDS             complement(18..374)
                     /locus_tag="PGA33_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDG6472582.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAK
                     DTKHNQAVVLQQLLNT"
     gene            complement(531..704)
                     /locus_tag="PGA33_000751"
     CDS             complement(531..704)
                     /locus_tag="PGA33_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472583.1"
                     /translation="MKLNQRYVKVFALYFVSIVTANIIVKNNNLIKTLIQTIAGYTVF
                     AVGLKYLTKRKNK"
     gene            complement(730..1875)
                     /locus_tag="PGA33_000752"
     CDS             complement(730..1875)
                     /locus_tag="PGA33_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HDG6472584.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGKNL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQV
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            2030..2137
                     /locus_tag="PGA33_000753"
     CDS             2030..2137
                     /locus_tag="PGA33_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472585.1"
                     /translation="MSVCTPTIERIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            2448..3401
                     /locus_tag="PGA33_000754"
     CDS             2448..3401
                     /locus_tag="PGA33_000754"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDG6472586.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYEEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(3721..5250)
                     /locus_tag="PGA33_000755"
     CDS             complement(3721..5250)
                     /locus_tag="PGA33_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDG6472587.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            5612..6547
                     /locus_tag="PGA33_000756"
     CDS             5612..6547
                     /locus_tag="PGA33_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDG6472588.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            6657..6755
                     /locus_tag="PGA33_000757"
     CDS             6657..6755
                     /locus_tag="PGA33_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472589.1"
                     /translation="MYVKNASKKHKTQKYMQKFQLLYFKRLKSQKI"
     gene            6886..8982
                     /locus_tag="PGA33_000758"
     CDS             6886..8982
                     /locus_tag="PGA33_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDG6472590.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLENHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDSISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRMHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            8967..9434
                     /locus_tag="PGA33_000759"
     CDS             8967..9434
                     /locus_tag="PGA33_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDG6472591.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            9457..9735
                     /locus_tag="PGA33_000760"
     CDS             9457..9735
                     /locus_tag="PGA33_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDG6472592.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            9962..11221
                     /locus_tag="PGA33_000761"
     CDS             9962..11221
                     /locus_tag="PGA33_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDG6472593.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQIICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            11239..12294
                     /locus_tag="PGA33_000762"
     CDS             11239..12294
                     /locus_tag="PGA33_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDG6472594.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAYHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKVLLKPLDLDENEGEN"
     gene            12296..12442
                     /locus_tag="PGA33_000763"
     CDS             12296..12442
                     /locus_tag="PGA33_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472595.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            12466..13509
                     /locus_tag="PGA33_000764"
     CDS             12466..13509
                     /locus_tag="PGA33_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDG6472596.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIIKVRATGICGSDTSR
                     YKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            14037..14753
                     /locus_tag="PGA33_000765"
     CDS             14037..14753
                     /locus_tag="PGA33_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDG6472597.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNVNGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            14746..15771
                     /locus_tag="PGA33_000766"
     CDS             14746..15771
                     /locus_tag="PGA33_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDG6472598.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEQHHIIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKTYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            15793..17487
                     /locus_tag="PGA33_000767"
     CDS             15793..17487
                     /locus_tag="PGA33_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDG6472599.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKENITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     FESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            17915..19084
                     /locus_tag="PGA33_000768"
     CDS             17915..19084
                     /locus_tag="PGA33_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol--glycerophosphate
                     glycerophosphotransferase"
                     /protein_id="HDG6472600.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQNAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGQASQRICQTIFK"
BASE COUNT         6609 a         2915 c         3670 g         5946 t
ORIGIN      
        1 acgataaaca tatataacta agtattgagc aactgctgta gtactacagc ttggttatgt
       61 ttagtatctt ttgctgcata taacaataga acatgattat gctgatttac aatatccttt
      121 aatttttcaa aagcatcttt ttgcgcatcc tgatcacgta attctttttc atatttttct
      181 ttaaaagctc caaaaagttt aggatcatgt tggaaccatt gtcgcaactc agtagaaggg
      241 gcaatgtctt ttaaccaata atctaggtta gcagttcttt tcgaaatacc tctcggccag
      301 actctatcga ctaggatacg aatagcgtcg gtattatctt tattgtcata aatccgtcca
      361 atatctacgg tcatcttgtg aactcctttc ttatgaaatt cagtgagcat acatcaatgc
      421 atgttgtggt gggacgacca aataaatttt gcgaaaatat catctctgtc ctactcccaa
      481 ttaaaaaaca gccatgacaa agtaaagtca tagctgtttt ggtatagatg tcatttattt
      541 ttacgtttag ttaaatactt caaaccaact gcaaagaccg tgtacccggc tatggtttgt
      601 atcaatgttt taattaaatt attattttta acaataatat ttgcagtaac aatacttacg
      661 aaatataatg caaatacttt tacgtaacgt tgattaagtt tcatatgagc actaaacctc
      721 ttttctcagt tatactgcaa cgcttagtct tggaataaat gtttcgtagt gtacgcgatc
      781 catatcgtaa tttaaagatt taagtgcttc gatcatagat tgtaagaatt ttgtaccacc
      841 acagatataa atttcaggtt tatttgctaa aaatacttgt aattcttcag caccaatata
      901 gccttgttta tcttttaagt gtgtatataa tttagcgttg tcatgatggc ttgcgatact
      961 gttgaagttg tctttgaaag gtaaatgttg ttcattttca gcaacttgaa ccatctgtgt
     1021 atctaaacct ttggcagagg cagcttcata catagctact aaaggtgtaa caccaatacc
     1081 tgaacctaag aaaagttgtg gttcagtcgt attctctaat acgaatccac ctacaggcgc
     1141 agctaaatta atcatatcgc cttctttaat ctcatcgtgt aaaattgttg aaacttcgcc
     1201 ttcatgttct gttgtgacat cacgtttaac gccaaaagtt aaatggtttt tttcacctga
     1261 tacgatagaa tagtgacgtt tagctctata tggaagttta tcactagaaa catcaactgt
     1321 gatgtattgg cctggtgtaa attcactaaa gtcatattct tcagtttcaa ctgtaaatga
     1381 tttaatgtct tcagattctt gtttaatatt ggtaattttg aatggtttaa aaccaatcca
     1441 catcatttga tcataaattt ctttttcaat ttggatgaac acatccgcaa taacgccata
     1501 tgcttttgcc caagcttgaa tgacagggtc attttcttct aatcctgtca cgtcttgaat
     1561 ggcttttaat aaatttttcc ccacaattgg ataatgttca gcataaactt gtagtgcgca
     1621 gtgtttatat gcgactggca taatgactgg tttaataaca cttaagttat cgatattaac
     1681 cgctgcggcc attacagctt gtgctaatgc tgaagattgc atgcctcgtt tttggttcgt
     1741 ttgattaaac atgtttaaaa gttcaggatg cgctttaaac atttttggat aaaagattga
     1801 cgtaatttct gtccctttct ctttaagtaa aggcaccgtt tgtttgataa tgtctttctc
     1861 ttgttctgta agcatgatac tcctccttta atctgtatat tttgattatt ctactaaaaa
     1921 tttcgatatt caattaattg gtttgagaaa atataaataa aatggcaaat ttgataattg
     1981 tatgacattt ttaatttttt aaatacttat caataagcat tgtgtacaat tgtctgtttg
     2041 cacaccgacg attgagcgca tttatttgac taattcaaaa aacattgttg ttttcctaga
     2101 aaaaagtaaa catgataata aaaatgtgaa agtgtaaata atcactggcg aagtacgaag
     2161 actaaagaca tctaagatgt aatcgtatac aaattaaaaa ggtgtaaaaa ttaaaataaa
     2221 atgtgaaata aatcacaatt taatattgac ccagtactta atgcatgtta cattttatat
     2281 gtgaaataaa tcacaaactt aaaagcggat gacacatgac cttttaagtt atgcgttgaa
     2341 aataaaagag atgtttattt gcttttgtat cgtcaataag cagcattaaa ctaacatatt
     2401 tgaagctaca tgtatgttag tgaattaatc ataagggagt ttttgtaatg aacaaattta
     2461 aagggaacaa agttgtatta ataggtaatg gtgcagtagg ttcaagctac gcattttcat
     2521 tagtgaacca aagcattgtt gatgaattag tcatcattga tttagacact gaaaaagttc
     2581 gaggagatgt tatggattta aaacatgcca caccatattc tccaacaaca gttcgtgtga
     2641 aagctggcga atacagtgat tgtcatgatg cggatctagt tgtcatctgt gctggtgctg
     2701 cacaaaaacc tggagaaaca cgtttagatt tagtatctaa aaacttgaaa atattcaaat
     2761 caattgttgg tgaagtaatg gcatcaaaat ttgatggtat tttcttggta gctacaaatc
     2821 ctgttgatat tttagcgtat gcaacatgga aattctctgg tttacctaaa gaacgtgtta
     2881 taggttctgg tacaatttta gactctgcac gctttagatt attgttaagc gaagcgttcg
     2941 atgttgcgcc acgtagcgtc gatgctcaaa ttattggtga acatggtgac actgaattac
     3001 cagtatggtc acacgctaat attgcgggtc aacctttgaa gacattactt gaacaacgtc
     3061 ctgagggcaa agcgcaaatt gaacaaattt ttgttcaaac acgtgatgca gcatatgaca
     3121 ttattcaagc taaaggtgcc acttattatg gtgttgcaat gggattagct agaattactg
     3181 aagcgatttt cagaaatgaa gatgccgtat tgactgtatc agcattatta gaaggcgaat
     3241 atgaggaaga agatgtttat attggtgttc cagcagtcat caatagaaac ggtattcgca
     3301 acgtcgtaga aatcccatta aacgacgaag aacaaagcaa gttcgcacat tcagctaaaa
     3361 cattaaaaga tattatggct gaagcagaag aacttaaata actttttata aaatctatac
     3421 catcccaaaa attgtaaaac cttacccaaa aaattgtata aagggctatt tgatacaact
     3481 atatgtgtcg agtagcccta tttttaatgt agtgaaagtc gttgttgaaa ttaaattcaa
     3541 atctggcact taagctttaa tcatagcatt aagatgattg tctagcagag gcgatttgcg
     3601 ggctcactac agtgcatgat gaacttaatg cttcaaatgt aacattaaaa ataaaagcaa
     3661 cgatgtcact tcttacttcg tacatcgttg ccattagttc tgttgatatt tcggttagtc
     3721 ttaatccccg agcaattctt caatttcatt tttgataact gtaacgtgag gcccataaat
     3781 tacttgcaca ccagtgcctt gctggattac acctttggca ccagtacttt cgagtaatac
     3841 tttatcgact ttgtcatttt gatgaagtgt gacgcgtagt ctcgttgcac aacagtcaac
     3901 gatttcaatg ttatctttgc ctcccaaacc agcaacaata gtttgtgctc tttcagtagc
     3961 ctcaacttgt tgtgctgcag ctttatcttc tcgaccaggt gttttgaaat taaatttcgt
     4021 aattaagaat ctgaaaacga tgtaatacaa acagaaccac acaattccaa taggtatgac
     4081 gtataggtag tttgttttac tattaccttg tagcacacca aagagtaaga aatcgataaa
     4141 gcctccactg aaggtttgac caattgtaat gttgaaaatg tctgccatca taaatgctaa
     4201 tccatcaaag aaggcatgga ttacataaag aataggtgcg acaaacaaga aactaaactc
     4261 taaaggttcg gtaatacctg ttaaaaatga agtgagtgca gcggataaca ttaaaccgcc
     4321 gacaactttt ttatgttcag gtttagctgt gtgataaatt gcaagtgcgg caccacataa
     4381 gccgaacatc atcgtaataa aacggcctga cataaagcgt gacacacctg aataatactt
     4441 cgtcacatct ggatcaccaa gttgagcaaa gaagatgttc tgcgtacctt gaactaagtg
     4501 ccctttgact tctaaagtac caccaagtgc cgtctgccaa aacggtaagt aaaaaatatg
     4561 gtgtaaaccg agtggaccta acaatcttaa gatgaagcca taaacaaaag taccgatggc
     4621 acctgttttc gttacaaatc caccaacatg ataaatgccg gcttgtatgc ttggccaaat
     4681 gaaaaacatc aatacaccta aaaagattgc ggcaaatgct gtgacaatag ggacaaatct
     4741 agagccacca aagaaaccta aatacggtgg taataccact ttgtgatatt tgttgtgaag
     4801 tattgcggtc ataatacctg tgataatccc gccaaaaaca ccggtttcaa ccgtttgtat
     4861 accgagcacc atgccttgtc cattttgtgc aagctgatct tttgccaatg tgcccgtgat
     4921 agttaataag ccattcatag ttgcgttcat aattaagaaa ccgagcagcg cagctaaacc
     4981 tgcagtacct ttatcgcttc tagataatcc gattgcgaca ccaattgcaa agatgaccgg
     5041 taaattttgg aaaacaatac tacctgcagc tgacattaat gtaaaaatat tttgtaataa
     5101 ggtaatatct aaaatagggt atgctttaac ggtgtttgga ttacttaatg caccaccgat
     5161 acccaacaat agacctgcag ctggtaagat tgcgataggt aacataaagg acttgccgaa
     5221 ctgctgtgct ttttcaaata aagatttcat caacatccct cctaattatt ctcaatatag
     5281 cttttgagaa atttaatatc aatatatatt ctgtgtatga aaatattttt cataaaaatt
     5341 gtttgaatca tgtaacaatc atataaattg ctgtttatat tgttgtgaaa atgagttgac
     5401 aaaagtcggt atagatatgt agacatccta tttttagcga ggtaggttga taaggcatat
     5461 cggataaatt tataagccat aaaatagata aatagtgttt gtatccaaaa atatgagaaa
     5521 gttaaaacta tttttcaaaa taaacatgat tcaccacata aataaatata cataagctat
     5581 cgataacaac ttaagaaagg tggatatata aatgaaaaga aagattatta tggattgtga
     5641 tccaggacac gatgatgcaa tagcattaat tttagcgggg gcaattgaca gtccactaga
     5701 gatattagct gtaacaacag tcgcaggtaa tcaatcagtt gacaagaata cgacaaacgc
     5761 cttgaacgta ttggatatta tgggacgcca agatatagca gtagcgaaag gtgcggatag
     5821 gccgttaatt aaaccagctg cctttgcttc tgaaatacat ggggaatctg gattagatgg
     5881 tccgaaacta ccgtcgacac catcacgtca agcagttgca atgccagcat cagatgtgat
     5941 tataaacaaa gtgatgacga gtgatacacc tgtaacaatt gtagcgacag gtcctcttac
     6001 gaatgtagca acggcattga ttcgtgagcc aagaatcgct gagcatattg aatctattac
     6061 tttgatgggt ggtggtacat ttggaaattg gacgcctaca gcagaattca atatttgggt
     6121 agatgctgaa gcagcgaagc gtgtttttga aagtgggatt actataaatg tgtttggttt
     6181 agatgtaaca catcaagttt tagccgacga tcacgtgatt gaacgctttg aaagtatcaa
     6241 taatcctgtt gcacagttcg tcgtagaatt attgcaattc tttaagaaga catacaagac
     6301 tcactttaat atggatggtg gtccaataca tgatgcttgt acaattttgt atttgttaca
     6361 accagaattg tttacaatgg tacccgttaa tatcgacatt gaacatcaaa gtccactaac
     6421 ttatggcact atggctgtcg atttaaatca tgttacaggt aagcctgcca atgcttattt
     6481 tgctacagca gttgatgttg aagaagtgtg gaacttgata gaccataagt tacgtacata
     6541 cgaataataa tacttaatta aatagataca gttaacccta aggcgcctga tataagcgtc
     6601 cttagggttt ttgtattggg ttacaatggt catgacaatt tgataatgat tgagatatgt
     6661 atgttaaaaa tgcttcaaaa aaacacaaaa cacaaaaata tatgcaaaaa tttcaattgc
     6721 tttattttaa aagattaaaa tcacaaaaaa tatagatata attgaaatgt tgttaaaaca
     6781 tcacaatgat gtacatgctc attttgaaaa tatgtgttca aagtgtgtac atcggtgtga
     6841 cgattgatga taaattgagc tcagatgctc aaaggagaat cgaacatgaa cggggataat
     6901 cagcaaatac tcagagaaat tgtattgaat cctactattc atggtaaaga acttgaatcg
     6961 atatttggtt tgtctcgtag acaactagga tatcgcattc aaaaaatcaa tttgtggctt
     7021 gaacaagagg gttatccaaa acttgaaaga acaagccaag gaaattttat tgtaagttct
     7081 gaaatcatga cgttattcaa gcgagatgta tcggagcagc aaatgttaaa cggcaacaat
     7141 gtcattttta gcatagaaac acgtcgttat tatttaatgc tcatgctttt tagtaaggaa
     7201 aacgcaatgt ctctaaacca tttttcaatt gatttacaag tcagtaaaaa tactgtcatt
     7261 cacgatataa atcatgtgaa agagcaattg gaaaatcatg gtttgtcact taagtattct
     7321 cgaaaacatg gttatgaaat tgttggtgat gaatttgaag ttcgccgttt cttcattaag
     7381 ttgattgatc aaaggttgaa tcatgatatt actaaaagtg aagttttaaa ggcgctcaac
     7441 ttaacattcg aagatatcgc atatcaaaaa gacaagatca aacaggtaga acaatttttg
     7501 aagagtcgct ttatagacaa atcacttagt tcattgcctt atgtcctttg tgtgattcgt
     7561 agacgaattc aaagtggtca tgtgatgaat ccattaaata ttaattatca gtatttgagg
     7621 gatacgaaag aatatcaagc aacggagatt atgacgcaac atgagccgga tttgccagaa
     7681 gcggaaaagt tatatttgac attacactta ctttcaacaa gtgtgcaatg gactgatttg
     7741 caagaatcag atagcatatc gaatttaacg atggccatcg ctcaaatgat tcaccatttt
     7801 gaacaaatca cttttattaa cattgaagat aaggagaaat tatcacagca actcttgtta
     7861 catttaacgc ctgcttttta taggattaaa tataacttaa cggatcgtga tgaattaata
     7921 aatcctttac aaggaaatta tcaatcctta tttcatatgg tgaaacaatc atgtcaatcg
     7981 ttaactgaat atttcggaaa atcgttgcct gataatgaaa tagcatattt aaccatgttg
     8041 ttcggaggta gtttgagacg tcaagatgaa aacttcgatg gcaagataaa agctattatc
     8101 gtgtgtacac aaggcacgtc agtatcacaa atgatgttat acgagttgcg aaacttattt
     8161 ccagaaatta ttttcttaga tgcgatttca cttagaacat ttgaaaatta cacattagat
     8221 tatgacatcg tcttttcacc aatgtttgtc ctaacacata aaaaattatt tatcacaaaa
     8281 gtagctttat ctgaaaatga gcaacgaaag ttacgtaaag aagtgatgaa gtacattaat
     8341 aaggaatcgg ctgacattga taaggaaata aacaagttaa tggcattaat tgaacgcaca
     8401 acgacagtta atgacattac agaactacgt gatggtttag aagattttat tgccaattat
     8461 aattcaattt caaccattaa tggatcgatt gtcacacaaa ataagacatt agatttagct
     8521 gacttgatac cggcaaggca cgtgaaaaga atgcatcatg ttgaaaatat tgatgaagct
     8581 attgctaaag caagtgatgt gttagttgct aatcatttta ttgatattaa atatattcat
     8641 gagatgcaac aggtatttga tgattcgtat atggttatca tgcaaaatat tgctattcca
     8701 catgcatact ctgaaaagca tgtacataaa acagcgatga gtatgttgat attacaagaa
     8761 ccaatataca tgtcagatgg cacagcaatc catattattg tacctattgc tgctgttgat
     8821 aaagtgacac acttaagagc gttactacaa ttgagagatg tggcgcaaga caatgacgca
     8881 attaagcgca tcatacaaag tcgcaaaaat tctgatgtaa atgagatttt aaaaaattat
     8941 tcaaataaag aagcgaggga aaatggatgg gacagcaatt agtgcataaa gaaaatataa
     9001 tgctcaattt gtcggcaact gataaagaat ccgtattgtc acaaatgtca gatgtgttat
     9061 ttcaaaatgg gttcgtgaag tcaacgttta aagatgcagt catcgacaga gaaaaagaat
     9121 ttgctactgg tttaccaacg catctatgtt cggtcgctat accgcataca gatgtcgaac
     9181 atattaacca tagaacgata ggtgtggctg ttctagaaaa agaagtgccg tttattgaaa
     9241 tgggaacact tgatcaacag acagaagtga aaatcgtttt tatgttagca atggataaag
     9301 tagatgatca acttaagctg ttacaacagt tgatgcaaat ttttcaaagt gaagaaaaat
     9361 tggagcagat tctacgaacg aaagatgaaa caattttagc aacactaatc aatgattatt
     9421 tggaatataa ctaaaaatta attggaggaa ttgaaaatga aacaagtatt agtagcgtgt
     9481 ggtgcaggta ttgcaacgtc aacagtagta aataatgcaa ttgaagaaat ggcaaaggaa
     9541 cacaatatta aagtagatat taaacaaatc aaaattacag aagttggacc ttatgaagac
     9601 actgcagatt tattagttac aactgcaatg acaaaaaaag aatataaatt cccagttatc
     9661 aacgcacgta atttcttaac tggtattggt attgaagaaa caaaacaaca aatcttaaca
     9721 gagttacaaa aataacggaa ttgatatgta acgtgggtcg ataacatatt aatatatgca
     9781 aattgcatat aaatgactat cacgacaaat gattgttgat gacatagaaa cgcagtgact
     9841 gtaattcaaa aggactgcag tcgaatttag caagggttgt cgttgaaatg actgtaacgt
     9901 caaatgtaat cacaatcgga aagtatcgtg acaatgcata tataacaggg agggtttaaa
     9961 tatgagttac ttcactgatt ttgtaagggg atttttagat ttaggtgcaa ctgttatttt
    10021 accggttgtc atattcttgc ttggcctatt ctttaggcag aaaattggag cggcatttag
    10081 gtctggttta acaataggtg tggcttttgt agggattttc ttagtcatcg atttattagt
    10141 taaaaattta gggccagcag cacaagcgat ggttaaaaat ttaggcgtca gtctgaatgt
    10201 gattgatgta ggttggccag caacatcatc tatcgcttgg gcatcatctg tcgcagcatt
    10261 tattattcca ctcggaatca tagttaacgt tgtattgcta gtaactaaag tgacaaagac
    10321 gatgaatgta gatatttgga atttttggca ttatacgttt acagcagcaa tggtttatgc
    10381 cgtatcaggc agtatttggc aagcgttatt agcagcagtt attttccaaa ttatctgttt
    10441 gaaagtagca gattggacag caccgatgat gagtgagttc tttgatttac caggtgtatc
    10501 gattgctaca ggaagtacaa tttcttatgc accaggtatt tacttagtta aattgttaca
    10561 aaaagtaccc ggtctgaata agttagatgc tgatcctgaa acaattcaaa aacgttttgg
    10621 cgcatttgga gagtctatat ttgtcggctt gattttaggt ttaggtattg gtgtgttagc
    10681 aggttacaaa cctggagaca tcattaattt aggaatgtca atggctgcag taatggtatt
    10741 aatgcctaga atggtaaaaa tcttaatgga aggtttaatg ccagtttcag agtctgcaag
    10801 aacatggcta aataaacgtt ttggcgaacg tgaaatttat attggattgg atgcggctgt
    10861 agcattaggt catccagcag ttatttcgac agcattaatt ttagtaccta tcactgtttt
    10921 attagccgtt attttaccag gaaaccaagt actacctttt ggtgacttag caacgatacc
    10981 atttgttgtc gcgtttattg ttggtgcagc aagaggaaac attattcatt ctgtcattgt
    11041 gggtacgatt atgattgcaa tttcactata tattgcaaca gacgtagcac ccattttcac
    11101 agatatggcg aaaggtacga atgtacaaat gccaaaaggt tcatctgaaa tttcaagtat
    11161 tgatcaaggt ggtaatatcg ttaactatct tatctttaaa ctatttagtc tattcaatta
    11221 aaaatcgagg tgtttgtggt gaaagcttta gtaaaaacaa gagaaggaca tggcaactta
    11281 gaacttcttg ataaagaagt tgcaacaccg ctagatgata aagtaaagat taaagtacat
    11341 tatgcaggaa tttgtggcac agatattcat acttatgaag gtcattataa agttaatttt
    11401 ccagtgacat taggtcatga attttctggt gaaatcgttg aagttggagc agacgttaaa
    11461 gattttaaag ttggtgaccg tgtcacatct gaaacgacat tctatgtttg taatgagtgt
    11521 gaatactgta aatcaaaaga ctataattta tgcaaccatc gaaaaggtat tggaacacaa
    11581 gttgatggcg catttactaa ttatgtcatt gcacgtgaag aaagtttgca tcatattcca
    11641 gacgaagtat cgtatcagtc tgcagctatg acagaaccat tagcatgtgc atatcatggc
    11701 gtttctaaga ttcaagtcaa ttcaggcgat gtagcagttg taatgggacc tgggccaatc
    11761 ggattacttg tagcacaagt gttaaaaagt aaaggcgcaa ctgttgtggt aactggattg
    11821 gacaatgaca aagtcagatt agataaagca gaagcattgc acatggatta tgtagtcaat
    11881 ttacaacaaa cagacttaaa aacgtatatc aatggaatta cagacggtta cggtgcagat
    11941 gttgttgttg aatgttcagg tgcagttcca gcagcacgac aaggtttgga tattttacgc
    12001 aaaaaaggtt tctacagtca aataggtatt tttaaggatg ctgaaattcc atttgatatg
    12061 gaaaaagtga ttcaaaaaga aataacagtt gttggtagta gaagtcaaaa gccagcagat
    12121 tgggaacctt cattgcaact tatggcggat ggtttagtaa atgctgaagc tttggtgaca
    12181 aaaatatatg atatttcgaa atgggacgag gcgtatcaac atttaaaatc cggcgaaggt
    12241 attaaagtat tacttaagcc gctcgattta gatgaaaatg aaggagagaa ttaatatggt
    12301 agaatcaatg ctaactttta tgcttgggcc attaagacaa atcactgatt tttatatgga
    12361 acatttactc gtaagtaatt ccattgtcat tgcaggttat tttgcgacag gtatttttaa
    12421 aaagaaaaaa gttgtgaatt aaatcaaatt tgaggtgatt tacaagtgaa agcattgaaa
    12481 ttatatggcg tggaagattt acggtatgag gataatgaaa agccagtcat tgaaagtgcg
    12541 aatgacgtta ttattaaagt acgagcgact ggcatatgtg gttcagacac gtcacgatac
    12601 aaaaaaatgg ggccatacat taaaggtatg ccatttggtc atgaattttc aggtgtagta
    12661 gatgccattg gaagtgatgt tacgcatgtt aatgtgggcg acaaagtgac aggttgccca
    12721 gcaatacctt gttatcaatg cgagtattgt ttaaaaggtg aatatgcacg atgtgaaaag
    12781 ttattcgtca ttggctcata tgaacctgga tcgttcgcgg aatatgtcaa attgccagcg
    12841 caaaatgttt taaaggttcc agacaatgtt gattacattg aagcagcaat ggttgagcca
    12901 tcagccgttg ttgcgcatgg gttttataaa tcgaatatac aacctggtat gactgttgca
    12961 gtaatggggt gtggcagtat aggtttgtta gctattcaat gggcacgaat atttggtgct
    13021 gcacatatca tcgctataga tatagatgcg cataaactag atattgcaac atcattgggc
    13081 gcacatcaaa caatcaattc aaaagaagaa aatcttgaga aattcatcga aaatcattac
    13141 gccaatcaaa tcgatttagc tatagaatca tcaggtgcta aagttacgat tggtcaaata
    13201 ttgacgctac ctaaaaaagg tggcgaggtg gtattactcg gaataccata tgatgatatt
    13261 gagattgatc gcgttcattt tgaaaaaatt ctgcgtaacg agttgacagt atgtggctct
    13321 tggaactgtt tgtccagtaa ttttccgggc aaagagtgga cggcaacctt acattatatg
    13381 aagacgaaag atattaatgt aaagcctatt atttctcatt ttttaccgtt agaaaaaggc
    13441 ccggagacat ttgataaatt agttaacaag aaagaacgat ttgataaagt catgtttacg
    13501 atttattagt atgcaccttt gaggacgaaa acgctggtat agttatagct atgaaagtgc
    13561 gaatgccgtc tggtctatag atactatcga aataattcat cttcgaatat acgttgataa
    13621 atagccggtt tacttgtgtg aaatatgctt gtgaatcggt tgttttgcat tttgtatact
    13681 taaaatgaga tggcaatatt tgataatttt taaagtgaaa atcaagtaca gccacttaat
    13741 aagataaatt tattataata tatggtaaaa tgatggcagt aataatgaat ttgaaaaaga
    13801 gtaaacatta atacctttaa caatttaata tcgtcagagt taatgattaa ctgcatggca
    13861 aaacaactta gaatggtcag ttacaaaaat acatttttat aaaaaattat cacactattg
    13921 tgacaactat ctttggatta ataaaagagg caagtgagca ataggttagg cttatgtgcg
    13981 ggcataggtc agtaatgtat aaatggaaat gatgtaatga cagaatggag gacaacatga
    14041 tttatgcagg tattttagca ggaggtattg gttcgagaat ggggaacgtg ccattaccaa
    14101 aacaattttt agatattgat aataaaccga ttttaatcca tacaattgag aagttcattt
    14161 tagtgagtga atttaatgag attattatcg caacgccagc acagtggatt tcccatacac
    14221 aggatatttt aaaaaaatat aacattacag atcaacgtgt caaagtagtt gcaggtggta
    14281 cggatcgaaa tgaaacaatt atgaacatta tcgaccatat tcgcaatgta aatggaatta
    14341 ataatgatga tgtgattgta actcatgatg ccgtaagacc atttttaact caacgtatta
    14401 ttaaagagaa cattgaagta gcagcaaaat atggtgcagt agatacagtc attgaagcaa
    14461 ttgatacgat tgtaatgtct aaagataaac agaacataca cagtatccct gtaaggaatg
    14521 aaatgtatca aggccaaaca ccacaatcat ttaatattaa attattacaa gatagttatc
    14581 gcgccttaag tagtgaacaa aaagaaatct tatcagatgc atgtaaaatc attgtcgaat
    14641 ctggacatgc agttaaattg gtacgtggag aactatacaa cattaaagtg acaacaccgt
    14701 atgatttaaa agtagcaaat gccattattc aaggtgatat tgccgatgat taatcaagta
    14761 tatcaactcg ttgcaccgag acagttcgac gtcacatata ataatgttga tatttatggt
    14821 aatcatgtca tcgtaagacc tttatacttg tctatttgtg cagctgatca aaggtattac
    14881 acaggtcgaa gagatgaaaa tgtactacgc aaaaaattac caatgtcatt agttcatgaa
    14941 gctgttggtg aagttgtatt tgatagtaaa ggggtatttg aaaaaggtac gaaagtagta
    15001 atggtaccga atacacctac agagcaacat catattattg cggagaatta cttagcctct
    15061 agttatttta gatctagtgg ttatgatggt tttatgcaag actacgttgt gatggcacat
    15121 gatcgtatcg ttccgctgcc taatgacatt gatttgagta cgatttcata cacagagtta
    15181 gtgtcagtaa gttatcatgc tatacaacga tttgaacgta aatctatacc tttgaaaacc
    15241 agctttggta tttggggtga tggtaactta ggttatatta ctgctatttt gctacgtaag
    15301 ttgtacccag aagctaaaac ttatgtattt ggtaagacag actataaatt aagtcatttt
    15361 tcatttgtag atgacatctt tacagtaaat caaataccag atgatcttaa aattgatcat
    15421 gcatttgaat gtgttggagg taaaggaagt caagttgcac ttcaacaaat agttgaacat
    15481 atttcaccag aaggcagtat tgctttgtta ggcgtaagtg aattacccgt ggaagtgaat
    15541 acacgattag tacttgaaaa aggattaacg ttgattggta gtagtcgaag cggctctaaa
    15601 gattttgagc aagttgttga tttatatcgt aagtacccag acatagttga aaagttagca
    15661 ttattaaaag gacatgaaat taatgtatgt acgatgcaag atatcgtcca agcgtttgaa
    15721 atggatttat cgacatcttg gggaaaaaca gtattgaaat ggacgattta ataaaccgat
    15781 gggggaataa caatgacaaa aacgaaacaa gcaatacata ttgataacat atactgggaa
    15841 cgtgttcagt tatatattga aggacatagt gaaggtgtcg atttaacatc aggacaattt
    15901 gttctgagga atttaaccga aacaaaaaca ttagaagcaa atgaaatgaa aatagacggt
    15961 aatacattta tatgtagatt caacgtcgca atattagacg atgggtatta tttaccaatg
    16021 gataaatatt tatttgttta tcatgaccag ttagagtata ttggacaact taatccaaat
    16081 attattgatc aagcttatgc ggcattaaat gaagagcaaa ttgaagaata caatgagctg
    16141 actacacaaa atggaaaagt gaactattta ttagcgtatg atgctaaagt tttccgtaaa
    16201 ggtggcgtat cacaacatac ggtctatacc attactccgg aaatagcaag tgacgttaac
    16261 gaatttgtat ttgatattga aatcacctta cctcaagaga aatcaggggt cattgcgaca
    16321 agtgcacact ggcttcataa acaaggtcat aaagcttcat ttgaaagtag aagtttctta
    16381 tttaaagcta tttttaatat tacaaagtta ctacatatta aaagaagcaa aacaatatta
    16441 ttcacatcag attcgcgtcc gaatttatca gggaatttca agtatgtata tgatgagtta
    16501 ctacgccaaa aagtagattt tgattatgat attaaaacgg tatttaagga gaatattacg
    16561 gatagacgca aatggagaga caagtttaga ttgccatatt tacttggtaa ggccgattat
    16621 atttttgttg atgatttcca tccattaatt tatacggttc gctttagacc atcacaagaa
    16681 attattcaag tgtggcatgc tgttggtgcc tttaaaacag ttggctttag tcgtacaggt
    16741 aaaaaaggtg gtccgtttat cgattcatta aaccatcgta gttacacgaa agcatatgtt
    16801 tcatcagaaa ccgatattcc attttatgct gaagcatttg gaattagaga agaaaatgtt
    16861 gtaccaacag gtgtaccacg tactgatgta ctatttgatg aagcttatgc aacacaaatt
    16921 aaacaagaga tggaagatga attgccaatt ataaaaggta agaaagttat tctattcgca
    16981 ccgacattta gaggtaatgg tcacggtacg gcacattatc cattttttaa aattgatttt
    17041 gaacgtttag caagatactg cgagaagcat aatgcagttg tgttattcaa aatgcatccg
    17101 ttcgtaaaaa atagacttaa tatttcacgt gaacatagac aatactttat cgatgtgtca
    17161 gatcatcgtg aagttaacga tattctcttt gttacagact tgttgattag tgattattca
    17221 tctttaatat atgaatatgc agtatttaaa aagccgatga ttttctatgc atttgactta
    17281 gaagattaca ttacgacgcg tgatttctat gaaccatttg aatcatttgt tccaggtaaa
    17341 attgtacagt cctttgatgc attaatggat gctttggaca atgaagatta tgaggttgaa
    17401 aaagttgtgc cattcttaga taaacatttt aaatatcaag atggtcgctc aagtgaacgt
    17461 ttagtcaaag atttgtttag acgctaatgt tggcatatat tacttgccat gcaaatgagt
    17521 cataaggcat agttttcaag aagggttctg acaatgaagt gagcaacatg tcatgatgag
    17581 aagcaggact atacaatgag aataaccttt tgatttttca tgcataaggc gattcaatca
    17641 aaagcaatca ccttccaact gaattgtcat tttgtaaaat aaaataatcg atccaatcgt
    17701 tatctaattc ataaatgtgt aaacacatac gttatgaatg gataacgatt ttttgttatg
    17761 ttaaagtggt acattaatca tgtatttcgt atgataatta acgacaagtg taatggttaa
    17821 atgtatttta tgatgaaatg ctataatagg catggttaca atgagcttgc tcatacatat
    17881 taatataatt acaaaaacac gtcggaggta cgacatgatt aaaaatacaa ttaaaaaatt
    17941 gatagaacat agtatatata cgacttttaa attactatca aaattgccaa acaagaatct
    18001 aatttatttt gaaagctttc atggtaaaca atacagcgac aaccccaaag cattatatga
    18061 atacttaact gaacatagcg atgcccaatt aatatggggt gtgaaaaaag gatatgaaca
    18121 catattccaa cagcacaatg taccatatgt tacaaagttt tcaatgaaat ggtttttagc
    18181 gatgccaaga gcgaaagcgt ggatgattaa cacacgtaca ccagattggt tatataaatc
    18241 accgcgaacg acgtacttac aaacatggca tggcacgcca ttaaaaaaga ttggtttgga
    18301 tattagtaac gttaaaatgc taggaacaaa tactcaaaat taccaagatg gctttaaaaa
    18361 agaaagccaa cggtgggatt atctagtgtc acctaatcca tattcgacat cgatatttca
    18421 aaatgcattt catgttagtc gagataagat tttggaaaca ggttatccaa gaaatgataa
    18481 attatcacat aaacgcaatg atactgaata tattaatggt attaagacaa gattaaatat
    18541 tccattagat aaaaaagtga ttatgtacgc gccaacttgg cgtgacgatg aagcgattcg
    18601 agaaggttca tatcaattta atgttaactt tgatatagaa gctttgcgtc aagcgctgga
    18661 tgatgattat gttattttat tacgcatgca ttatttagtt gtgacacgta ttgatgaaca
    18721 tgatgatttt gtgaaagacg tttcagatta tgaagacatt tcggatttat acttaatcag
    18781 cgatgcgtta gttaccgact actcatctgt catgttcgac ttcggtgtat taaagcgtcc
    18841 gcaaattttc tatgcatatg acttagataa atatggcgat gagcttagag gtttttacat
    18901 ggattataaa aaagagttgc caggtccaat tgttgaaaat caaacagcac tcattgatgc
    18961 attaaaacaa atcgatgaga ctgcaaatga gtatattgaa gcacgaacgg tattttatca
    19021 aaaattctgt tcattagaag atggacaagc gtcacaacga atttgccaaa cgatttttaa
    19081 gtgataactt aaaaacaata aaaaattata aattaattag ttaagtgata taaataataa
//
LOCUS       DAMQYT010000026        18540 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMQYT010000026 DAMQYT010000000
VERSION     DAMQYT010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18540
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.026"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..1341
                     /gene="brnQ"
                     /locus_tag="PGA33_000769"
     CDS             <1..1341
                     /gene="brnQ"
                     /locus_tag="PGA33_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDG6472601.1"
                     /translation="TFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIANLGFL
                     ITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPRLATTS
                     FEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLVLLAII
                     LLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATIKKLGI
                     ENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQITQYYLG
                     NYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLFANVGL
                     TKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKLIYQCTIFFTMIAALVDGLKASP
                     EFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQA"
     gene            1594..2778
                     /gene="rocD"
                     /locus_tag="PGA33_000770"
                     /pseudo
     CDS             1594..2778
                     /gene="rocD"
                     /locus_tag="PGA33_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
     gene            2817..3848
                     /locus_tag="PGA33_000771"
     CDS             2817..3848
                     /locus_tag="PGA33_000771"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDG6472602.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            3860..5101
                     /gene="argJ"
                     /locus_tag="PGA33_000772"
     CDS             3860..5101
                     /gene="argJ"
                     /locus_tag="PGA33_000772"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDG6472603.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            5117..5881
                     /gene="argB"
                     /locus_tag="PGA33_000773"
     CDS             5117..5881
                     /gene="argB"
                     /locus_tag="PGA33_000773"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDG6472604.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            6151..6630
                     /locus_tag="PGA33_000774"
     CDS             6151..6630
                     /locus_tag="PGA33_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDG6472605.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(6958..7602)
                     /locus_tag="PGA33_000775"
     CDS             complement(6958..7602)
                     /locus_tag="PGA33_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDG6472606.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(7615..14790)
                     /locus_tag="PGA33_000776"
     CDS             complement(7615..14790)
                     /locus_tag="PGA33_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDG6472607.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKVKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYFPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     VTPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIAVEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPETIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSE
                     LNVAQLRQAVQRLIARHEILRTQYIVVDDEVRQRIVADVAVDFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHHCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQVEQDLKQYLNDQLPKYMIPKT
                     ITHIDCMPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMYQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGNTLLTGATGFLGAYLIEVLQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEIMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTNPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            complement(15237..16487)
                     /locus_tag="PGA33_000777"
     CDS             complement(15237..16487)
                     /locus_tag="PGA33_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDG6472608.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGVFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(16873..17898)
                     /locus_tag="PGA33_000778"
     CDS             complement(16873..17898)
                     /locus_tag="PGA33_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HDG6472609.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKALGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(18071..18430)
                     /locus_tag="PGA33_000779"
     CDS             complement(18071..18430)
                     /locus_tag="PGA33_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HDG6472610.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
BASE COUNT         5980 a         3525 c         2851 g         6184 t
ORIGIN      
        1 acatttaaag aaaacatgtt tataggttct atgttatttg gtttattctt tggtgccggc
       61 aatcttatct tcccaataca cttgggtcaa gctgctggtt ctaacgtttt tatcgctaac
      121 ttaggatttt taattacagc aattggctta ccatttctag gtatcattgc tattggcatt
      181 tcaaagacat ctggtttatt tgaaattgca tcgcgtgtta ataaaacata tgcttacatt
      241 ttcacgattg ccttatatct agttatcgga ccatttttcg ccttacctag actggcaacg
      301 acatcatttg aaattgcatt ttcgccattt ttatcaccaa agcaaatcac tttatattta
      361 tttattttta gcttcgtctt ctttgtgatt gcatggtttt ttgcgagaaa gccatcaaga
      421 attttagaat atatcggtaa atttttaaat ccggtattct tagtattatt agcaattatt
      481 ttattatttg cttttatcca tccattaggt ggcatatctg atgcacctat tagtaaacaa
      541 tatcaatcac atgccttatt taacggcttt ttagatggat acaatacctt agatgcgcta
      601 gcgtcattgg catttggtat tatcattgtt gcaacgatta aaaagttagg tatcgaaaat
      661 ccaactgata tcgctaaaga aacaattaag tctggtacta tcagtatcat tatgatgggg
      721 atcatttata ccctactagc aatcatgggt acattaagta ttggtcattt caaacttagt
      781 gaaaatggtg gtattgcctt agcgcaaatt actcaatact acttaggtaa ctacggtatc
      841 gtcctgttgt cacttatcgt tatggttgct tgtttaaaaa cagccatcgg tttgattacg
      901 gcattttcag aaacattcga acaccttttc cctaaaatga attacctagc gattgcaaca
      961 gttgtaagct ttatttcgtt cttattcgcg aatgttggtt taactaagat tattatgtac
     1021 tcagtcccag tgttaatgtt cttatatcca ttagcaattg ccttgattgt actaacatta
     1081 tttagtagca aattccatca ttcaaaactt atttatcaat gtaccatttt ctttacaatg
     1141 attgctgcat tagtagatgg attaaaagct agtccagagt tcatttcaag cacatcattc
     1201 tcacaaactt tgattaattt cagccaaaaa tatttaccat tatcagacat tggtatgggc
     1261 tgggttgttc tcagtttgat tggtttcatt atcggcttca ttatttataa aattaagcat
     1321 cgtaaaattc cacaagcata atactatgcc acagtcatat gttaaacata tgcttgtggc
     1381 attttttatt catactacat taaactgcaa tcgtatacat acatatcaat gattatccac
     1441 aaaaaatatt agtactttca ttttacaaat cacattaata caaacacaac cttatcttta
     1501 tattattaaa tttacatttg acacttatat tgaacaactg taatatatta atattaattc
     1561 tttaaaatgt ataaatataa aggagggaga ccgatgaatt caatcattga attaactgat
     1621 tattatagct ctaataatta tgcaccactt aagcttgtca tttctaaagg taaaggtgtc
     1681 aaagtttagg atactgatgg caaacaatat atagattgca tttcgggttt ttcagttgca
     1741 aaccaaggcc attgtcatcc aacaattgtt aaagcgatga cagaacaagc ttcaaagttg
     1801 tctatcattt cacgtgtcct ttatagtgac aatctcggga aatgggaaga aaaaatttgt
     1861 catcttgcta agaaagacaa agtactcccc cttaactctg gtactgaagc tgttgaagca
     1921 gccattaaaa ttgctagaaa atggggctct gaagttaaag gcattactga cggacaagtt
     1981 gaaatcatcg ctatgaataa caattttcac ggtcgtacac ttggctcatt atcactatct
     2041 aaccacgacg catataaagc aggatttcac cccctacttc aaggcactac aacagtagat
     2101 tttggagaca ttgaacaatt aacacaagct atttcaccga atacagcagc aattattttg
     2161 gaaccaattc aaggtgaagg tggcgttaat ataccaccga aaggatatat tcaagctgtg
     2221 cgtcaactat gtgataaaca tcaaatatta ttgattgcag atgaaattca agttggtctt
     2281 ggtagaactg ggaaatggtt tgctatggaa tgggagcaag tcgttccaga catttatatt
     2341 ttaggtaagg cattgggtgg cggcttatac cctgtatctg ctgtacttgc aaataatgat
     2401 gtcatgcgtg ttctaacacc aggtacacat ggttcaacat ttggtggtaa ccctttagcc
     2461 attgcaatat cgacggcagc gcttgatgta cttaaagatg aacaactggt tgaacgatca
     2521 gaacgcttag gttcattttt attaaaagcg ttgctacaac ttaaacatcc tagtattaaa
     2581 gaaattagag gtcgtggttt atttataggc atagagctta acacagatgc tgcacctttt
     2641 gtggatcaac tgattcaacg tggaatctta tgcaaagaca cgcatcgtac tatcattcga
     2701 ttgtctccac ctctagtcat tgataaagag gaaatccatc aaattgttgc agcttttcaa
     2761 gacgttttta aaaattaaca attaatcatt tatatatgac ataggaggga ttcatgatga
     2821 ttaaagtagg tatcgttggc ggtagcggtt atggcgcaat tgaattaatt cgattgttac
     2881 aaacacatcc tcatgtaacg attgcacaca tctactcaca ttcaaaagta gatgaaccgt
     2941 tgaaattaac atttccacat ttacaacata ttatgcaaca tttcgaagca cttacagtgg
     3001 acaataatga ctgtgatgta attttctttg cgacaccagc acccgtaagt aaaacatgta
     3061 tccctccctt agtagaaaaa ggtattcatg ttatcgattt atctggcgca tttagaatta
     3121 agaatcgtga aatatatgaa gcatattaca aagaaactgc tgcagcacaa gatgatttga
     3181 atcatgctat ttacagcatt tcagaatggc aatcgtttga taacaatgga acgaagctca
     3241 tttctaatcc tggctgtttc cctacagcaa cattattagc attacatcca cttattagcg
     3301 aaaaaatagt agatttgtca tctattatta ttgatgctaa gaccggcgtg tcaggtgctg
     3361 gtcgttcatt atcacaacgt gttcattttt cagaaatgaa tgaaaatcta agcgcttatg
     3421 caatcggaaa ccataaacac aaaccggaaa tcgagcaata tttatctatc attgcgggtc
     3481 aagatgtatc agtcatattt acaccacatc tcgtaccaat gacacgaggt attttatcaa
     3541 caatatatgt caaattatca tctgaatata cgactgaatc attacataaa ttaatgacct
     3601 cttattatgc taatcagcca tttgtcagaa ttagagatat tgggactttt ccaaccacaa
     3661 aagaagtact cggtagtaac tactgcgata tcggcatcta tgtagatgaa acaacgcaaa
     3721 cagcaatttt agtatcagtg attgataacc ttgtcaaagg cgcaagtggg caagccattc
     3781 aaaatttaaa tatattatat gattttgaag tgacgactgg cctaaatcaa tcaccagttt
     3841 atccataagg ggtgttagaa tgaaacatca agaaacgaca tcacaacaat ataacttttc
     3901 aattattaaa catggcgata tcagtacacc tcaaggcttc acggctggtg gtatgcacat
     3961 cggtttacgc gctaacaaaa aagactttgg atggatttac tcatcgtctt tggcaagtgc
     4021 agctgccgta tatactttaa atcagtttaa agctgcacca cttattgtca ctgaagacac
     4081 tttacaaaag tctaaaggaa aattacaagc acttgttgtt aattcagcta atgcaaattc
     4141 ttgtaccggt caacaaggca tagatgatgc acgacaaaca caaacatggg ttgctcaaca
     4201 acttcaaata ccatctgagc atgttgctgt tgcttcaact ggggtcattg gtgaatattt
     4261 gcctatggat aaaattaaga ctgggaccga acatattaag gatgctaatt ttgcaacgcc
     4321 aggtgcgttt aacgaggcaa ttttaacaac tgatacctgt acaaaacata tcgctgtatc
     4381 actaaaaatc gatggtaaaa ccgttacaat tggtggtagc accaaaggtt caggtatgat
     4441 tcacccaaat atggctacca tgcttgcttt tataacaacc gatgcatcga ttgaatcgaa
     4501 tacacttcat caattattaa aatcttcgac tgaccataca tttaatatga ttactgttga
     4561 tggcgataca agtacaaatg acatggtatt agtcatggca aatcaccaag ttgaacacca
     4621 aatacttagt caagaccatc cacaatggga aacatttgtt gatgcattca attttgtctg
     4681 tacattttta gctaaagcta tagccagaga tggcgaaggc gcaacaaagt taatcagcgt
     4741 caatgtgtca ggcgctaaat caatatcaga tgcaagaaaa atcggtaaaa ctatcgtaag
     4801 ttcaaatcta gtaaaatcag ctatttttgg cgaagatgcc aattttggtc gaatcattac
     4861 agctattggc tacagcggat gtgaaattga tcctaactgc acatatgttc aactgaacca
     4921 aatacctgtc gttgataaag gtatggctgt actatttgat gagcaagcta tgtcgaatac
     4981 attaactcat gaaaatgtca caattgacgt tcagcttggt ttaggtaacg ctgcagcgac
     5041 tgcatacggt tgtgatttat cctatgatta tgtgcgtatc aacgcatcat atcgaacata
     5101 aggtggtgtt ggttagatga aatttattgt cattaaaatt ggtggcagta cacttagtga
     5161 catgcatcca tcaattatta acaacattaa gcatttacga tcaaacaaca tctaccccat
     5221 tatcgttcat ggcggtggcc catttattaa tgaagcatta tcaaaccagc aaatcgagcc
     5281 acactttgtt aatggcctaa gagtgactga taaagcaacc atgaccatta ctaaacacac
     5341 gctcattgca gacgttaaca ctgcattagt agctcaattt aaccagcacc aatgttctgc
     5401 aataggctta tgtggtttgg atgcacagct gtttgaaatt acatcttttg atcaacaata
     5461 tggatatgtc ggtgttccga ccgctttaaa taaggatgct ttacagtatt tatgtactaa
     5521 atttgtacct atcatcaatt cgattggttt caataaccat gatggagaat tttacaatat
     5581 taatgctgac acgcttgcct attttattgc atcatcatta aaagcgccta tttatgtatt
     5641 aagtaatatt gcaggtgtac tcatcaatga tgttgttata cctcaattgc cattagtcga
     5701 tattcatcaa tatattgaac atggtgatat ttatggaggt atgattccca aagtgctaga
     5761 tgccaaaaat gcgattgaaa atggctgtcc taaagttatc attgcatcag gaaacaagcc
     5821 aaatatcatt gaatctattt acaataatga ttttgttggc acaacaatcc ttaattcata
     5881 actatgaaat taaggcctaa caagttttga cacgcgagat gattccagtt cgattatcca
     5941 ttgcgctaaa acatttattt accgttcatc tcgttaacaa ttttgaatac agtacgatac
     6001 aatatgagat gtaaaaaact aataaccttt tacaaatttg tttatcaaaa tattttaagt
     6061 tttgcaaagc tttttattgt gattattttc acaaaatact ataatgagga tagtaaatag
     6121 agaggagtcc ttaagttgac gaaacgacaa atgggtatat tcatttatgc tggaattatc
     6181 ggtggcttgt tatctggaat tgtaaaatta ggttgggagg tcatgtttcc acctcgcaca
     6241 ccagaacgta atgcaacgaa cccacctcaa gagttattgc aacaattagg atttagtagt
     6301 gagtttacgc atcaaacata tacattttca aatatggaat tgccttgggt aagctttatt
     6361 gtccacttta gtttttctat cgtcattgca attatttact gcatattagt taaaaaatac
     6421 gcttacttag caatgggaca aggtgctgtt tttggtattg ctatttgggt attattccac
     6481 cttatcatta tgccaatcat gcatactgta cctgctgtgt gggatcaacc attccaagag
     6541 catctgtcag aattctttgg ccatatcgtc tggatgatga caattgaatt agtgcgacaa
     6601 cattttgtct atcgctataa attaaattaa tacactgact aacattaacg tgagttttaa
     6661 atcatcgttt gagtatgatg attgatgctc acgttatttt attaactgac atgatatgat
     6721 tccagccaac ttacgtgagc attaaagtct caaatgcgtc gtaacaaact attattttcg
     6781 gtaatttcaa tattgctcag tatattttta ccttatcact tactttaatc tcgtcatgat
     6841 tttgaatgat gccatcgtgt attcaccttt catttttcca ataaaaaaac atctaacagt
     6901 aaacatttag gcagtatagt ttaaaatcac tgcgcaatga tactgtcaga cgtcatatta
     6961 actactcaat aactgaaata cagacacttt tttataaccc cagggtgcct gtcctaagaa
     7021 acatacctgt accataaact gatcaaaaat aaattgtttg aacttcactt cacgtgattg
     7081 ataaaagtgt gattgtgtca tatcataaat gtccaatcct ttgattaaac cttcaccaat
     7141 caattttgta aaactttctt tttgtgtcca tatttgataa aaatcattta aactacatat
     7201 ttgatgtgct tcgtttgtag agaaacacgt cactaacgta cgccagtcta aacgttgtga
     7261 tatcttttcg atatcaatac caactggttc tttatcgaca acacacacga tataaggata
     7321 actatatgat aagctcacat agatgggctg tccatcacga ttgtgttgaa caatatctgc
     7381 cttacctcgt ggcgaaatgt gataatgcca ttcatgtggt aataaacctg tgtcatgttg
     7441 aattccatat tgcactaaaa tatctcccaa tctgtgcatg agtttatctt gattgtatct
     7501 atagttgact gtacgcggtt ttttatatga ccaacgactt tgtgatatta attcttcaat
     7561 acttttcaag ttactctgta attgcattac aaatactgtc ataactttcc ctacttactt
     7621 attgaatatt gttttgatat attgtgccca atgatacagc caattgttag ttatcgttgg
     7681 ccatttttca ctgatgtgat tcattatttt taatgttaat gttgtatcta tcattgctag
     7741 ttgttgttca cggtcaacac tagttaatcc aatcgtttcg tacatgtctt gtttctgtaa
     7801 aatttcatta aatgattcat cgctgacgag ttcaatttct ttgcgcttaa cgcattctaa
     7861 caaagatttc accggcattt tattaggtga tagcacatgg taaatgattt gtggtgtgtt
     7921 gacctgtgct aatgcgacaa tttgtcttgc agtcgtatcc acaaaagaaa aatctacagg
     7981 catttcagcc atgctaaccc cgatacaatc cagttgtaac aaatcattca ttaccattga
     8041 aaaacggtta gtctttatat ttctcatatg ccatcttcca ttgtaaggat tcgtcaaatt
     8101 accaacacgt acaatccgac catctaagcc attatttaca gcttctaata cttttaattc
     8161 actataaaat ttgctccgtg tatatggtga tgttagtagt tgccctttat agacatccgc
     8221 ttctgaaaat gtcacatctt ctgtgtctat atcaaaataa gttcccacac ttatcgtaga
     8281 cacatatatt aaccttgcat gatgttgttg tgccaaacgt atgacatcaa cagtaccttg
     8341 aacatttact ttttcaaatt catcatcatc accaaagtga tctgtacgag cacctgcatg
     8401 aataatcgta tccatgtttt ctggtaaaac aacatcatcc atacactcga aatcaccaac
     8461 aatgacttca atgtttgata acattatttc aaccgtctct tctgaaaaat aatcatttaa
     8521 attcgtcatc aacttatacc atgctatttc ctcattatca gcacgtatga aacaataaat
     8581 gcgatgactg tatccttgta gtacttcaat cagataagca cctaaaaaac ctgtcgcgcc
     8641 agtcaatagt gtatttccta gaggtcgatg acttagacta tcctctaaaa tacccaagtt
     8701 ataacgagac ataacaatct tttgtaattc cgaaagatta tccggtaatg caactaatga
     8761 ttgttgattt tggtacatat aattaacaat ctgtcgcacg gttttatatt ggtataatgt
     8821 ctgcattgaa atatgatggc caaatcgttt taaatgcgag acaactaaca tcgcctctaa
     8881 tgagttacca ccaagttcaa agaaatcatc gtcaacaccg acatcatttt gtttcaatac
     8941 ctctccaaat acatcaacaa atgtctgctc aatttcatta gagggttcgc tatacacttt
     9001 attagactgt tgtataggtg atggatttgg caaacgcgta gtatccacct tgtcattcgt
     9061 ggttaatggc atacaatcga tatgcgttat agtcttagga atcatatact taggcagctg
     9121 atcatttaaa tattgcttca aatcctgttc cacttgttgc tctccgacat aataagcatt
     9181 caatatatca tgcgtatcaa agtgacttac tgttacaaca caatcagata taccacgaat
     9241 agctaatatt gcattttcaa tttcatcaag ttcaatacgg tacccgttaa ctttcacttg
     9301 tttatctatt cttcctaaaa attcaatttg accatcagat gtataacgtg ctaaatcacc
     9361 actatgatac aactttcctt taccaaatgg attattttgc catttatcag ccattaattc
     9421 tggacgatta atatatccta tcgctaaact atcacctgca atacacaact cgcctggcat
     9481 accaataccg cataacaaac catctgacat aatatacact tggatgttag ataagggttt
     9541 gccaattgga atcgtctcag gtatcaaatc accacaatga tgtgaccaat acgatgtgat
     9601 gactgttgac tcagatggtc cataggcatt gaaatacgtg ccacaatgct tctcaatata
     9661 tttaacaaag gatgccgtac tagttgcccc gcctgtaatc aacttttcaa tataaaagtc
     9721 ttccataaca ctacacatct gtaacggaat cgacgcaacc gtcacacgat gcttattaat
     9781 gagttgttgt aactgttctg gattaacacg ttcctctcta tctggaatca caagcgtatg
     9841 accatttaac aaacaacaat aaatctccat aactgatgca tcaaaaacaa tatttgcatg
     9901 ttgcaaaaat acttcattgt cgcctaattg caattcagtt gaccatgcat gcactaaatt
     9961 caacaaattt cgttgtcgta tggcaacccc tttaggcatc ccggtcgtac cagatgtgta
    10021 aatagcatac atctcattat ctaacatcgc tgtgttttca agttgattgc catgtaaatc
    10081 atcatattgt tcattttcct ttgattcaac aaagccttta gcattttcca attgaatgac
    10141 tggtaatgtc gtttcaattt caacgccgta agacatgact gcagttactt tagcatcctc
    10201 caaaattgca ccttgtcgtt tattcggaaa atcaatatcg ataggtatat aagatgcacc
    10261 tactttaact gtcgccaaca tcgccgcaat catttcaaaa ctacgttctg taaacaaggc
    10321 aacccgttga ccattgccca caccatttga taggagcatg tgcgcaatgg catccacata
    10381 gttgcgtaat gtttcatacg tcattgtcaa atcattcatg actagcgcaa catgattacc
    10441 ttgtcgtgag acaacttcat taaagtaact tatgatagat ttatttcccg ggacattaag
    10501 cattcgatcg ttaacatgcg tattgaccca atttagaagt tcctccgtgc cgtttggtat
    10561 atcacaaatt tgtagtgtat cttgatgctt caaaatataa tcaatcataa tcatacattg
    10621 attacccatg tgacgaactg tttctgagtg atataaatcg gtattatact cgatattgat
    10681 tgtatagtca tcgcgatctt cttcaatgat gaaagataaa tcaaatttcg ccgtcactga
    10741 tttgggttga atgtgtgtta atttactatg cccaaaatga gcatgattcg tttcattgtt
    10801 ttgtagtact aacatgacat caaataatgg attccgtgag gcatcatgtg attgatctaa
    10861 gtcatttact aaacattcga atgggtattc ttgatgctcg tatgcctcca aactcatttc
    10921 cttaacctct tgtaaaaact gtgtccacat tttatcaggt gacggttgcc ctctatatac
    10981 caacgtatta gcaaacatgc ctagcatttg ctccgtgcct ttatgcatac gcgcactcat
    11041 cacactaccg acaacaacat catcttttcg agcatatcta cttaacaacg tcatgaccac
    11101 actcataaag aacataaaat cagtaatttg atgcttttct acatactttt gaagtagctg
    11161 tctcatttgt tgattcattg taaatgacat cattgctcca tttgtcgttt taatatttgg
    11221 tctaacatag tctgtcggta agcttaaaat aggtacttca tctttgaatt gagataacca
    11281 atattgtcta tgtttcgtca tatcacgatg cgacatccac tcactatagt ctttatattg
    11341 caatttaagt ggtaacaata atttatgttg ataaagtgcg ttaagatcat tcattaattg
    11401 tatattactc ataccgtcat taatgatatg atgcgtatct ataaagaggt atgcatgtaa
    11461 gggacttcta atgtatctca ctctaatttg acttggcttt tccaaattaa aaggtgctac
    11521 aaattggcgc atgatttctt gttcatccgt aaaatgcgtg ttaacttctt caaagtcaac
    11581 tgcaacatct gccacaatac gttgtcgaac ctcatcatct acaacaatat attgtgttcg
    11641 taaaatctca tgtcgcgcta tcaaacgctg cactgcttgt cgcaattgag ctacattaag
    11701 ttctgatgat aaccgccata aaaaaggtac gttatacacc gtatctttat ggtttgattt
    11761 ccataataaa tacatacgct tttgtgcaga gctcagcaca taatcatctt taactatagt
    11821 ttctggaatc acttcatagt tttgttcttg aaccttagca atcgcttgtg ctagttcaaa
    11881 tacagttggc ttttgtaata aatcaccaat ttgtaatcgt ttcccagtag atgcctctat
    11941 ccgattcacc actaacgttg cttttaatga atggccacct aattcaaaga aattatcatg
    12001 aatacctact tgattcacat gtaaaatatc tgcaaaaatt tggcatagca agtgttcggt
    12061 atctgtactc ggtgctacat aggcatccgt atcgacatag tccatgatag gcaatgcctt
    12121 cttatctaat ttcccattaa tagtaatagg aatttgctca atatgcatga aattaactgg
    12181 tatcatgtac tccggtaagg tcatacgtaa ttgtgattta atcttattat gtgataatgt
    12241 atgcatcgct tcataataag caacgatata ctgatcttga tcatgatttt gaacaataac
    12301 aactgcttta ttaatacctt gtatacgctc gagcgcatgc tcaacctctg acaactcaat
    12361 cctaaaccct cgaatcttaa cttgtttgtc ctttcgatat aaataatcta tgttgccatc
    12421 gggtaacaaa cgaacgatat caccacttct atacatcagc tgatttatat ttgaatcttt
    12481 gataaattta tctgctgtca attctggctg atttaaataa cctgcagcta acccaaagcc
    12541 acttgtacat aattctccag gaataccaac gccacaccga cgctcgcctt gcatgatata
    12601 aacatgagta cccagaatcg gtttaccaat aggaatacga tttggaactt tgttaggtat
    12661 attatacgtc gttgtaaatg ttgtattttc agttggtcca taaccattaa taatttgagg
    12721 atgcttcggt ttttgattaa gcaaatccac ccacttagca ttcaatactt ctccaccaat
    12781 taataaatac tttaacggta ccaatacttc tattcgttca ctagcaatct gattaaataa
    12841 tgaggaggtt aaccacatag tattaacgtc attttcattg attaattgtt ctaccgctat
    12901 tggatttaat aattgttctt ttttagcaac aatcagcttt ccaccattga gcaatgcacc
    12961 atatatttca aatgttgcag catcaaaggc tatagttcct gataacaaaa tcgtcgtctc
    13021 ttcatttaat ggtacataat gattttgatg gaccaatcga acaatacctc ggtgcggaat
    13081 tagtgtccct ttagggttac cagttgtccc cgacgtgtaa ataacataag catgatcttc
    13141 taacgtgtta catttagaaa gattatcaat atttttccac gctatcttat tcaaatcaat
    13201 gtgattaata ttttgtttac cattttcata taaagcttgg tacgttatta caactttagg
    13261 cgttacatct tttaaaatgt actcctgacg atcacttgga tagttcggat caattggcac
    13321 gtaagcccca ccagctttca acacacctat catcgctatt atcatctcaa tacttttttc
    13381 agctatgaca gcgacacgat cattaggttc aacaccatac tggtttctca aacggtgtgc
    13441 taaatcattc gcgcgtgcat tcaatgtttg atatgttata aacactccgt caaattgcac
    13501 agcgacatga ttcggcgttg cttcaacttg ttgctcaaat aaggtaacaa ctgtttgcgc
    13561 atcatctatc tcaggcaaac ttaaattgat atcgtcatat aattgaatat cacgttctgt
    13621 catcaaatta agttcatcta cagttgttcg tttatttcca ttttcttcag taatttgcaa
    13681 ataaatattt cgaactaagt cactcagcgt ctcgattgag agcaaatcat atgctgcact
    13741 gttatatata attttgaacc cgtgttgatg tggaaaaatt tcaatatccg ctaaagatgt
    13801 atgtgcatca tgtatttgat gtacatcctc aataacatca ttacaacaag acatcatatg
    13861 atgataacaa tgaaaaatag tctctagtga aagcgaagac ttcgcgcact gtaattgcga
    13921 catattttgc aacacacatt tattaaaatc tgttgtaaaa cgttgacata catcttttgc
    13981 atcgattgtt aacgttaacg gcacaatatt tccgtgtaaa tcatttggta aatgtgatgg
    14041 tacatgtata cctaatgtga catcatgttg ttgactcatt atatgattag ctaaatacac
    14101 actaacagcc aacgatgcca tatctattga tgtcatatca tcaatcaaat acgtttgata
    14161 taaagcttgt tcaaatggat gcttaattgg aaaataacta tcaatatgga tgtcagagtt
    14221 attctctaac cgaaaatagt ttgagtctaa tgctatatgc gatgcatctt gattgtcttt
    14281 atcatcattt ctatttatat gtgcatgctg tcgagtattg ttaataacag tattgccacg
    14341 atatgcattg cataaatcat caagaaaaat atcaatttga ctatcatcga aaatggacac
    14401 atgaaaatct aatagtatat atgcagcatc agcgaactga aacaatttaa ctttgaataa
    14461 aggtgaatca ttaaaatggt aagtacttaa ttcttgctta aaaaaagctt ctaaatcata
    14521 gtttgcggaa gaagatggaa cttgttttat ctcaataaaa ggcagaaatt catgaagtat
    14581 cattgttaaa ttgtcatcgg tagtaacatc aaaaaaatgt cttatagatg catgttgttg
    14641 cacaattgtc gataatgcat acatcatttt agtagcttca acatttttag cgagtttaac
    14701 ccaatacgca ttacggtgtg tcgttgattc tgtattattt ttgtatatac gaaaatattc
    14761 ctgttgaaat ctcaaattac ccataatcat aaaaagtcct tctttcatat cataatactc
    14821 attacttact gaaattgcat gatgatatga taaccgacga aatgttaatt aactcgttat
    14881 gtaatgatta atataaaaca ccattcgcaa catatgagcg atatattcta ccctaaaata
    14941 catcttgtat catcgttaca attggtatat ttttcaatgt aaattacata catcttcgat
    15001 aaatagcaca ctacaactcg ttaatcactt tctgttgttc acatctcatt gcaaactcaa
    15061 tattgttgtt acaaaatatc catgaagcaa gtttatatta aacaaacaac tcgcataaaa
    15121 caattgttat ccttaaattt taacaaattc ttaataaatt tatctctatt ttaattacga
    15181 ccaaattaat aggttttcca tataaaaaga tgcataaaat aaatatttaa ataaattcaa
    15241 tttgtattca cttgtttttg tcccccaaat acaccagcaa caagcatgct agcaccaatt
    15301 gttaaaacga taaacatata cagtcccatt tgtaatgacg ttaagaaaac acccaacaca
    15361 atccctaacc tagctagtgt ttctgaaaaa tgaataccta atgcattaac tgcactatat
    15421 gttcctcttt tagctttagg aataatttta aagcgttgtt ctgaaactat aggcgaataa
    15481 ataatttcac ctacagtcgc aattatcata aaaacaacta ataagccaaa ctgattaaga
    15541 taggttagac cactatagcc aacaatataa atcagcaaac cagtgattaa agctttttta
    15601 aaatctattt tcaatacaac tttcgagatt gaatacgtga gtaaaatgac gacgaccgta
    15661 ttaatcatta gcaagattgc taacatctta gcacctgtaa tatcatatga acctatactt
    15721 attgtttcaa actgatcctt tagtctaata gcaatatatg aggagattga aaattcaccc
    15781 atcatgatga tactgaaccc cgaaatcaat aacatataat tacggtcttt caaaactaat
    15841 ttataactgc gaaatatatt cattatttgt aatttttgat aacgacttgc atgcctcttg
    15901 tcatcacttt gctttacttg atttcggtct tgaggtaacc aaatatataa aataaagagt
    15961 acaattaaaa atatacaagc tgctattaag aaaagtagta acatactgta gccatacatc
    16021 aagccaccta acaatgcccc aatagctacc gataagtttg tcatccaata gctaatcttg
    16081 taaatataat gttccacgtc ttcggtaatt gcatccataa ttaatgtgtc cataactgga
    16141 aattgtaatc cccaaacgat tgtaaatatg gcatatgcaa cacaaaaacc aataatttgc
    16201 cacaattgat gtgacccaaa tacgcccatg aacacaagca ttatcaccat cgtcgcttga
    16261 taaataagta ctagcaactt tttcggaaat atctcaataa ggtaaccaga tataatggac
    16321 aatggaaatt ttagaaccac taaaccaaca agatatatac cgacaattga ttgacttaac
    16381 atatctgtta aatatagtgc tataaacggt ataaatgctg tcgtaataat tagctgtaaa
    16441 aaattgctaa tcaatcgtac tttcaaagtc gtacttaagc gtttcattaa taactacacc
    16501 cctttccaca tatatacaca ctaccattta tataaaatct attattagat tatacgttga
    16561 aacaaagaat ttttagttat acaaagttat ttaacaaaca aatgcatcga ctttacaatg
    16621 catcttgcat ataatgcctc aaaaaaatta gcactatatg actatatatt attccaaatg
    16681 aatgaaattt atcaataatt gcgtaagtga acacatatgt gcgtaaatgt taaatcattg
    16741 tcctaacata gctgtgacta tacttagatg taatcataaa aaaactactg ccatctttac
    16801 aacagcagta gtgcttatat gattcattta tataaaattg aaagcgttaa tcgctagccc
    16861 agcatcctta ttctatttag ctgtataact tttactagca atacgaccac ttgcaacaat
    16921 aatatcttta tcttggaaag gttcatgatt gaagaaacgc tctaaaatat ctttaactcc
    16981 atcttcaata cgtttttgtg cttctaaagt catacctgaa tagtgaaccg tcatagcatt
    17041 tctaggcatt gttctccatg gatgatcagc aggtgcaggt tgtggatacc aaacatcacc
    17101 agcatatcct tgtaaatgct cggatgctaa cgcttcaact aacgcatcgc gatttacaat
    17161 tttaccacgt gcagtattca ctaaataact gtgttttttc atacgactta aaacatcttt
    17221 atcaaataag ttatcagttt ctggtgttaa tggtgcatga attgtaatcg catcacttgt
    17281 tgaaacaagt tcatcaaagc ttacaaattt agacaattta tggtcttgtt gattgattgg
    17341 atcatagtgt tgtaatgtta cattaaatgg cgctaatctt tcagcaacaa gttgtccaat
    17401 tcgaccaaat ccaaaaatac caattgtttt gtgttgtaat tcatgcgcat gattacctac
    17461 ttgagacaag ttccattcac cttctactga ttgacgatga ccttcttcat agtttctaag
    17521 aagtattaat aaatccataa ccgcatgttc tgccacacta actgtattac ttcctgtaac
    17581 ttcaacgaca ccaatattgt gttcacttgc tgccgctaaa tctacatggt cagatcctac
    17641 acctgctgta attgctaatt tcaagttcgg tgctttttca atacgttcac gagtcatata
    17701 tgcaggataa aatggcgcac taataatcac atccatatct ggtaaatgtt tatctaagtc
    17761 ttcaccatta tctgctaata taatgaactc atgtcctctt tcctctaaaa atgtttttaa
    17821 tcctaatgct tttttagtat taagtaattg attttcttga ccttctactg cttctggaaa
    17881 taatgctacg attttcatat ccatctctcc taaatgataa attttgactt ccttaaagta
    17941 cgtgttgcat gttgcttgtt ttaaaaatat gacaaaaaaa cggcaccgtt tgacatacca
    18001 cccccttgaa ttattgttaa attcaaagcg aagttagcaa acagcaccgt gtaccttaca
    18061 ccagcaacac ttattctatt gacttatcga aaacaaatat tgaaatgact tcatcatcat
    18121 tcaaatcaat atctgtaaat aattttacaa acttagcgcc tacaagactt tccatctcat
    18181 ttacatctat ttctttatac atctgtttaa tcttctctgt gcgtgtataa tggagcatcg
    18241 attcattgcg tttgtcgttt aggtaaaaac tctcaacttt actcaaaaca cctgtcatat
    18301 gtgcaatcgc ccaattatct ttaaacgaca ctcgaatact attgggtcct ttacccacgt
    18361 attctttacg ataagatctt actagattcg agaattgttg ttctttagta tgcgtatcta
    18421 tagtattcac cttctttaaa ataagaagtt cattaattgt tcacattttc attatacacc
    18481 ttattaacac tttttcaatg ataacgatta ttattattac gaaatgtata ttttcctagt
//
LOCUS       DAMQYT010000027        18302 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMQYT010000027 DAMQYT010000000
VERSION     DAMQYT010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18302
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.027"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            164..1162
                     /locus_tag="PGA33_000780"
     CDS             164..1162
                     /locus_tag="PGA33_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDG6472611.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            1394..2275
                     /gene="rbsU"
                     /locus_tag="PGA33_000781"
     CDS             1394..2275
                     /gene="rbsU"
                     /locus_tag="PGA33_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HDG6472612.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGLPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            2290..2694
                     /gene="rbsD"
                     /locus_tag="PGA33_000782"
     CDS             2290..2694
                     /gene="rbsD"
                     /locus_tag="PGA33_000782"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDG6472613.1"
                     /translation="MKKSAILNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            2722..3636
                     /gene="rbsK"
                     /locus_tag="PGA33_000783"
     CDS             2722..3636
                     /gene="rbsK"
                     /locus_tag="PGA33_000783"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDG6472614.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKVAHIDTSYIIKTAEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSIGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(3734..3868)
                     /locus_tag="PGA33_000784"
     CDS             complement(3734..3868)
                     /locus_tag="PGA33_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472615.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESTSIGLVENYF
                     "
     gene            3887..4648
                     /locus_tag="PGA33_000785"
     CDS             3887..4648
                     /locus_tag="PGA33_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDG6472616.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYNQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKEH"
     gene            complement(5082..6518)
                     /locus_tag="PGA33_000786"
     CDS             complement(5082..6518)
                     /locus_tag="PGA33_000786"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDG6472617.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(6534..7325)
                     /locus_tag="PGA33_000787"
     CDS             complement(6534..7325)
                     /locus_tag="PGA33_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDG6472618.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDTQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YTPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAVGIQSEDGVDIVIHIGLNTVDLEGKGFKCFVKQNDHVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            7474..8178
                     /locus_tag="PGA33_000788"
     CDS             7474..8178
                     /locus_tag="PGA33_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDG6472619.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEI
                     ATPPKSVQDELQLNADDTVYYLERLRFVDNDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDQLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(8286..8987)
                     /gene="lrgB"
                     /locus_tag="PGA33_000789"
     CDS             complement(8286..8987)
                     /gene="lrgB"
                     /locus_tag="PGA33_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HDG6472620.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(8980..9423)
                     /gene="lrgA"
                     /locus_tag="PGA33_000790"
     CDS             complement(8980..9423)
                     /gene="lrgA"
                     /locus_tag="PGA33_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HDG6472621.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(9536..10276)
                     /locus_tag="PGA33_000791"
     CDS             complement(9536..10276)
                     /locus_tag="PGA33_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HDG6472622.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNNASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPTYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(10279..12033)
                     /locus_tag="PGA33_000792"
     CDS             complement(10279..12033)
                     /locus_tag="PGA33_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDG6472623.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(12280..12954)
                     /gene="scdA"
                     /locus_tag="PGA33_000793"
     CDS             complement(12280..12954)
                     /gene="scdA"
                     /locus_tag="PGA33_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HDG6472624.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAAALEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQSAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGELLVKMSELTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(13094..14818)
                     /gene="tarS"
                     /locus_tag="PGA33_000794"
     CDS             complement(13094..14818)
                     /gene="tarS"
                     /locus_tag="PGA33_000794"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDG6472625.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKNQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFKGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISLKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVQILLSKDIKKRDFKNIVKLNTA
                     HITYPLNK"
     gene            complement(14851..16539)
                     /locus_tag="PGA33_000795"
     CDS             complement(14851..16539)
                     /locus_tag="PGA33_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDG6472626.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            complement(16561..17586)
                     /locus_tag="PGA33_000796"
     CDS             complement(16561..17586)
                     /locus_tag="PGA33_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDG6472627.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            complement(17579..18295)
                     /locus_tag="PGA33_000797"
     CDS             complement(17579..18295)
                     /locus_tag="PGA33_000797"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HDG6472628.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
BASE COUNT         5862 a         3430 c         2790 g         6220 t
ORIGIN      
        1 tctctcgtct caaccttaat cgtatacttc agacgtctgt ttgtagacaa taaaagtcat
       61 tcacgtcttc atatgtcatc aaatgtttat catgatatga tgaatataat aatcgggtat
      121 ataactgtat gattaattac acaataaaga gaaggaatat aatatgaaaa aagtgtcaat
      181 taaagatgtt gctagagaag ctggtgtatc agttacaact gtgtcacata ttttaaatca
      241 taatgatagt cgtttttccg caacaacgat aaaaaacgta catgctgttt cagaacgttt
      301 aggctatgcc cctaataaac atgcaaaaca attgcgcggc agtaaaattc aaactattgg
      361 cgtcattttg cctagcttaa caaatccgtt tttctcagca ctgatgcaaa gtattcatga
      421 ccataaacca tctgatgttg atttatgctt tttaacatct acagcaactg atttgtatga
      481 caatattaaa catttaattg atcgaggtat tgacggatta attatcgcac aatacatatc
      541 atccccggac gccctaaata actatctaaa gaaacatcat gtaccttatg tcgtactgga
      601 tcaaaatgac catcaaggct atacagattt tgttcggaca aatgaatatc aaggtggaca
      661 acttgcagca caacatttaa tagaactcgg tcacaaccat atgataattg ttgcaccata
      721 tgacatgatg gcgaatatgt cgactcgtgt cgctggattt gtcgatactt tgcgcgcgaa
      781 tcaattgcca gaaccacaaa tcgtccatac tgaattatct aagcgcggtg ggctaaccat
      841 tgttgatgac atcatggttc aatctgccac tgcaatcttc gctattaacg atgaactcgc
      901 tattggcatt ttacgaggac taattgaaca tggcatcagt atcccgaaag atatctcatt
      961 aataggttat gacgacattg attatgcagc gtacgtctcg ccacctttaa ctactgtggc
     1021 acaacctata actgatattg gcaaaacatc tttaacctta ttacttcaac gattacagca
     1081 cttagataaa tccattgata tgattgaatt accaacgact ttaaaaattc gtgcaacaac
     1141 tggctatcat ctttcaaact aactacgtat cttccgaaat atactcatca ttgttaggcc
     1201 cttagcgttg ctttaatgct gagggttttt aatcataatt attttactaa gaaattaaaa
     1261 taataatgta tgaattttta aatatgattt aaacgttttc agtttttatg aaaacgcatg
     1321 cattttacaa ataaaaatgg tacgatggca ctggtaaaac gttttactaa aaacaaatca
     1381 tgaggtgtat aacatgagca ttgttgcatt acttatcggg ttaggcccct taattggctg
     1441 gggcttcttc ccaacagtcg cttcaaagtt tggtggtaaa cctgtacatc aaattatcgg
     1501 tgctactgta ggtacgttaa tcttcgctat tatattagcc gtagtcacat caagtggctt
     1561 acctactgga accaatttgc tattcgcctt attatcaggt gcaggatggg gattcggaca
     1621 aatcattaca tttaaagcgt tcgaattagt cggctcatct cgtgccatgc cagtcacaac
     1681 agcattccaa ttattaggcg catctttatg gggtgtcttt gcattaggaa attggccagg
     1741 cattggtcat aaaatcattg gatttacagc tttagtcgtt attctaattg gagcgcgtat
     1801 gacagtttgg agtgaacgca aagaagcaag taacgccaaa aatttacgtc gtgcagtggt
     1861 acttctgtta attggtgaat ttggatactg gttatattca gctgcaccgc aagcaacttc
     1921 tattgatggc ctaactgcct ttttacctca agcaatgggt atggtaattg ttgcagtcat
     1981 ttatggcttt atgaatatga aagcagagaa tccattccgt aataaaatta cgtggttaca
     2041 aattatttca ggtttcttct ttgcatttgg tgctttaaca tatcttattt cagcacaacc
     2101 taatatgaat ggtttagcaa ctggatttat tctttctcaa acatccgttg tgcttgctac
     2161 attaactggt atttatttct taaaacaaca taaaacgtca aaagaaatgg ttattacaat
     2221 catcggctta gtactcattt tagtagccgc ttctgttaca gtatttataa aataaggagt
     2281 gtagatgtca tgaaaaaatc agctatttta aatgaacata tttcaaaagc aatcgcgaca
     2341 attggtcatt ttgatttatt aacgattaat gacgctggca tgccaattcc aaatgatcat
     2401 cgtcgtatcg acctagctgt aactaaaaac ttaccacgct ttattgatgt cttagctaca
     2461 gtgttagaag aaatggaaat ccaaaaaata tacttagcag aagaaataaa agaacataac
     2521 cctacacaat tgcaacaaat taaacaattg atttcatcgg aaatcgaaat cattttcatt
     2581 cctcacgaag aaatgaaaag taacttagct cacccattaa ataaaggtaa tattcgtact
     2641 ggtgaaacaa cgccctactc taatattgca ttagaatcga atgttacttt ttaaaagtta
     2701 taacttgaaa ggagcgtaca catgaccaac aaagttgtta ttttaggttc aacgaatgtc
     2761 gatcaatttt taacagttga aagatatgca caaccaggcg aaacattaca tgttgaagaa
     2821 gcacaaaaag cattcggcgg aggtaaaggt gccaaccagg ctattgccac tgcacgcatg
     2881 caagcagaca caacatttat tactaaaatt ggcactgatg gcgttgctga tttcatctta
     2941 gaagatttta aagtagctca tattgataca tcatatatta tcaaaacagc tgaagcaaaa
     3001 acgggccaag cctttatcac tgtgaatgca gaaggacaaa acaccatcta tgtttatggt
     3061 ggtgcgaata tgacgatgac acctgaagat gttattaacg caaaagacgc tataatcaat
     3121 gcagactttg tcgttgcaca attagaagta cccatcccgg ctattatatc tgcatttgaa
     3181 attgccaagg cacatggtgt gacgacagta ttaaatcctg caccagcgaa agcattacct
     3241 aatgaattat tatcattaat cgatattatt gtgccaaacg aaacagaagc cgaattgtta
     3301 tctgggatta aagtaactaa tgaacaatct atgaaagaca atgccaatta ctttttatct
     3361 ataggcatta agactgtttt gattacgcta ggtaagcaag gtacatattt tgctactaaa
     3421 aatcaaagcc aacacatcga agcttataaa gtaaatgcga ttgatacaac tgctgcaggc
     3481 gacacattta ttggtgcatt tgtcagtcgc ttaaacaagt cgcaagataa cttagcagat
     3541 gctattgatt ttggtaataa agcgagctca ctcactgtac aaaaacacgg cgcgcaagca
     3601 tctattcctc tactagaaga agtaaatcaa gtttaaatga atcaaacaca gctatgatat
     3661 gaaggtttag catataacat gcaacattcg tatatcatgg ctgtgctttt ttatctttat
     3721 aaaacatcat ctattagaaa taattttcca ctaaacctat gcttgttgac tcatgtttag
     3781 ttataaatga agtgacaatt ttttgtaatc tttttaactt ccaaattatc gcatataaat
     3841 atgctatatt aatgataata attatcaatt aaaaggaggt tatgctatgt ctaaagaagc
     3901 tggtcataca tttttagcta aattaggaaa aactcgtcta cgccccggtg gtaaagaagc
     3961 aacagattgg ttaatacaac aaggggcatt ttcacaagat aaacaagtgt tagaagtggc
     4021 atgtaatatg tgcacaacat ctatttatct agctcataca tatggctgtc acattcaagg
     4081 cgttgatata aataagaaag cattagaaaa agcacaggaa aacatttcag cagcaggtct
     4141 tgaatcatat attcaagttc aacaagcgaa tgctgttaaa ttgccctttg atgacaatca
     4201 attcgatatc gttttaaatg aagcaatgtt aacaatgtta cccatcgcca taaaggaaaa
     4261 agcattacgc gagtactacc gagtcttaaa gcctgggggt atcttgttaa cacatgatat
     4321 tgtcatcgtt aatgaatcac atgccacaca tgttgttaaa tcattatctg cagcaattaa
     4381 tgtcaatgtc tcaccgcaga cgaaacttgg ctggttagat ttatataatc aagctggttt
     4441 taatcatgtg cattatcata ctggtccaat gagtttaatg acaccaaaag gtttaattta
     4501 tgacgaaggt attgttggaa ctataaagat tatcaacaat gctttgaaaa aagaaaatcg
     4561 accaatgttt tgtaaaatgt ttaaaacgat gactaaattg cgtaaagata tgaattatat
     4621 tacttttgtc gctaaaaaag agcactaaat ataatgccac taactgtact ttgtatctat
     4681 gtttgactat cactttaatt tctttgtgac actaatcatc tacttaacaa tatcgttatc
     4741 gttgattagt aagtcatcaa ttttggttaa agactttcat aaacactcaa acattaacac
     4801 tatacatagt tagtggcatt atttttttct taaaatttta acatcacggg attggaacag
     4861 aaatgatatt ttcgcaaaat ttatttctgt ccttccccaa cttgcattac ctgtagaatt
     4921 tcttttcgaa tttctctgtg ttggggcccc gccaacttgc attacctgta gaatttcttt
     4981 tcgaaattct ttgtgttggg ggcccgccat tataacactg taggtcctac tatattaatt
     5041 tatgtctcgg gctctaaccc taaaatactt ataatatacc tttataattt atctccattt
     5101 gatgctatca cttgttgata ccaatagaac gaatcttttt tcaagcgttc catcgtgcca
     5161 tgaccatcat tatctcgatc aacatatatt aaaccatagc gtttcttcat ttcaccggtt
     5221 gtaaatgaaa caatatcaat gattccccac ggtgtataac cgattaaatc tacaccatct
     5281 tgatcaactg cttctatcgc tgctgtaata tgtgctttta aatattcaat gcgataatca
     5341 tcatgaatat gtccatctac cacttcatca actgcaccaa aaccattttc cacaataaaa
     5401 agtggtaact gataacgatc atataacaca ttcaatgtat atcttaagcc atctggatca
     5461 atcgcccaac cccaatcact cgttgcgata tgcggattct ccacagaatg attcaaacca
     5521 ccgttgacga tgttgttttc aactgtagta tcaacatcat gttttacagc cgtagacatg
     5581 taataactaa agccaatata atcaaccgtg ccctgctgta aaatactgtc gtccccttct
     5641 tgccatccaa catcatatcc ttttcgtgcc aacatttttt tagcatagct tggataataa
     5701 cctctcactt ggacatccgg gaaaaagaaa cgtaagcgat tcgcaatttg tgcttccatc
     5761 atatctttcg gatgacacga atatggataa atgggtacat gtgaaatcat tgttccaatc
     5821 ttaaacttcg gattaatctc tttaccaaga cgaactgcta aagcactggc taaaagttca
     5881 tgatgtgcta cttgatacaa gacttcttca ggattatcat tttctgtcaa tgctacccca
     5941 gaattcgtcc ataaaaagat aggatttgat gtgtccatct gattattaat ttcattaaac
     6001 gtcatccaat atgtaacttt atctttatat ctttcaaata caacacgcgc aaaatgcaca
     6061 aaataatcga caacttctct atttctaaat ccaccataat gtttcgctaa atgaagtggc
     6121 atctcaaaat gtgataacgt cacaacaggt tcaatacctt gtgcaattaa ttcatcaaag
     6181 atacgatcat aaaaggcgag tccttcttca tttggcacat cttcatcccc attcggaaag
     6241 atacgtgtcc acgcaatcga cgtacgtaaa catttcaatc ccatttcttt aaacaaggca
     6301 atatcttcct tataacgatg ataaaaatca ataccttcat gatttggata atagtgattg
     6361 ggatctatag attctgtaat ctgacgtgct ttgccatgtg caccactcgt cataacatca
     6421 attacactta accctttacc acctttatca tatccacctt caaattgatt tgcggcaaga
     6481 gcgccacccc acatgaaatt ttgcggtaat tttgtcatta ttacaacaca ctcctagttt
     6541 ttaaatattt taaaaataac atcttcagtc gtaatgattt cattcattgt cagttctact
     6601 tttcctaaat cggcagagtt gctaataacg acaataacgt cagcattgta gccttgttgt
     6661 tgtatatatt gctggtcgaa ttgcaacaac gtttgccctg cttcaacatg atcattttgc
     6721 tttacaaagc acttgaaccc tttaccttcc aagtcaactg tattcaagcc aatatgaatg
     6781 actatgtcca caccgtcttc tgattgaata ccaactgcat gcttagttgg tacaatcata
     6841 gatattaaac cattgaacgg tgcgattact ttggattctt catgagcttt gattgctaag
     6901 ccttcaccta ccattttctc tctaaaaatt gaatctctta cttctttaag taaaatacga
     6961 cgaccagcga ttggtgcttt cactaaaatt ggtgttgtta cttttacttg aggtgtataa
     7021 cttgtatctt gactattaat attgtcagta ttctcactct cttcgacatt agcattctta
     7081 attaatcgct gtaattgctg atacacttca tcgacatttc catttaattt tatgactaac
     7141 tgcgtatcat tttctgctac agatgtcaca tcaactattt gtttcacttc atttgctgta
     7201 gaaggaattg tatgttgcat atgaattgta atgcacttcg tatcatacgt tgcatcaaca
     7261 atgttttcga caccacctac agctgtaaga atgtcttggg cttgttcttt atatttattg
     7321 ctcataaata ttaccccctc aacttgatag tagaatgaaa atgtatttac ttacctccaa
     7381 ttataattgt accggttcaa tttgtaaacg ccgatacaat tataatattt tgtgctataa
     7441 taattacaga caaagtgaaa cgaggacaga atattgttaa agtatgaaca tattgctaag
     7501 caacttaatg cgtttataca tcaatctaat ttcaaacccg gtgataaatt gccaagcgtg
     7561 acgcaattaa aagaacgtta tcaagtaagt aagagtacta tcattaaagc attaggctta
     7621 ttggaacaag atggtttgat ctatcaagca caaggcagtg gtatttatgt gagaaatatt
     7681 gctgatgcca atcgtatcaa cgtctttaag actaatggtt tctctaaaag tttaggtgaa
     7741 caccgaatga caagtaaggt acttgttttt aaggagattg caacgccacc taaatctgta
     7801 caagatgagc tccaattaaa tgcagatgat accgtctact atttagagcg attaagattc
     7861 gtggacaatg atgttttatg tatcgaatat tcttattatc ataaagaaat cgtgaaatat
     7921 ttaaatgatg atattgctaa gggctctatc ttcgactatt tagaatcaaa catgaaactt
     7981 cgtattggtt tttcagatat tttctttaat gtagatcaac tcacttcaag tgaagcttca
     8041 ttactacaat tgtctacagg tgaaccatgt ttacgttacc accagacttt ttatacaatg
     8101 actggcaaac cctttgattc atctgacatc gtatttcatt atcgtcatgc acagttttat
     8161 attcctagta aaaagtaata aatacataaa aacgtctata tcccagttat aaactggagt
     8221 atagacgttt ttttacgata ataacaatgg ctcaaattgc tattatcttg cttaggtttt
     8281 tcgttttaga agaatattgc tacaaagaca ggcacaactg ctacaacaac tacaccaact
     8341 aacactaaag ctatacttgc cattgattct tctacaggtc ctaattcttt ggctggtgct
     8401 acacctaatg tgtgaccact tgttccaagt gctaatcctc gggcaatagg gttagtaatt
     8461 cggaaaagct ttaagaattt attacctagg gcataaataa tgacaccatt taaaataact
     8521 gctaatgatg ttaattcttt tataccaccg ataccagctg atactggtaa cgcaatcgct
     8581 gtagttgctg cttgaggtaa cattgataaa ataacatcat tggcaaattg tgctaacttc
     8641 gcaaaagtta aaataattaa taacgctaca actgtaccga taccaatacc tccgatgata
     8701 cgatgccaat gtttaacaag cacttcacgc tttttatata acggaatcgc aaaacagatt
     8761 gttgccggtt ctaagaagaa gtaaataatg tctccaccta ttttgtaagt cttatacgga
     8821 atgcctgtta aatagaggaa ggccacacca aataccatac tgacaaatag cggtgcgaat
     8881 aagaagaaac gattagtttt ttcaaataat atggtcgcta agaaaaatgg tataacggat
     8941 aacagtattc cgaagtaagg tgtgtttagt gctaagtggt taatcatgag cttgtgcctc
     9001 ctctattttg atcttttttg tgactttgtc acctttagat ctcgaagtaa ctttcataat
     9061 aatttgtgtg acatagccag tacaaataag taatagtatt gttgagacga ttattagtcc
     9121 aatgattaaa aatggtgctt ggctaatgac acctaaagag ttaacaactg agataccggc
     9181 tggtacgaag agtaagccaa tgttatttgt tagtgtcgtt cctacttttt cgacttcgcc
     9241 taacttaaca gcaccagtac ataataatac aaataataat actaaaccga ttactgatgc
     9301 aggcatagga attggcataa atgattcaat tattttcgat acaaagagta ctaaagcaat
     9361 tacaatgact tggtgaaaaa agtgtgctgg ttttgatgcg tctttttgtt gtttcacgac
     9421 cattgcctcc tacgtttgat ttaactaaag tatagatggc tcacttcgat ttgcgtgatt
     9481 tttagtccga aatacaaaat atcataggta aaatgcataa aaaaaaggat tactgttaaa
     9541 gtaatcctat cgacgcttta aaatctttca taaatgaacg tccaacttgc atcttgacac
     9601 catttgtcaa tattaccata taagtatagt taaaccattg ttgcacttct ttaatgtgtt
     9661 tcgtgttaat aatatatgaa cgatgaatac gtataaaata agtgggattc aatcgttttt
     9721 cataacgatt caatggctct gttgtttcgt atttatgatt cgttgtatgt atggttgtaa
     9781 taccattatg tgtgccaatc ccaataatat tttgttgctt taacatgtga attttatcgt
     9841 caatttcaac aggtaagctt tgatcaaaat tcgccgacat atcattcgca attgcacttg
     9901 cgttattatc atctttggct ttagtcgcac gcactttatt gactgcttgt tcaatacgtt
     9961 tttgaccaaa cggtttcaaa atatagtctg tcgcatttaa ttcaaatgcc tgtactgcgt
    10021 attggtcatg tgcagttgca aaaataatcg caggtggctc tttcatcttt tgaatcttag
    10081 ctcctaattc gatcccattt tcatccatta aattgacatc taaaaatata atgtcatatt
    10141 gattgatcag tagtgcttcc aatgtttctt ttacattttc tgcctcatta atttcttcaa
    10201 aaccaccaat ttcatttaat aaatatgtta attcattacg tgctaatggc tcatcatcta
    10261 tgattaatgc tttcatattt attcctcctc ttgtctttca taaggaagta cacaccaaaa
    10321 agtggtaccg ctcgatgtcg attcaaattg taatgctgcg gattttccaa ataatccttt
    10381 taggcgtaag tttaaatttt ctaaagcact accagttcca gactctgatt ctacagatgt
    10441 ttctcccaac aaatgcattt tatctttaga aataccctga ccattatctt gtacaataat
    10501 acgtacatgt gttgcagttt ctttaatcac tgacacgtca atatcgttac cttgctttcg
    10561 attcgtaaac gcatgtttga tggcattttc aactaaaatt tgaattaaaa atggtggtac
    10621 aagcacatcg cgatatttgt cttcaacatt aatattgata ttaaatcttc ctggataacg
    10681 tgcttgttcg agtgataagt atgcacgcac ttgacttaac tctttatcta aagtaatcgt
    10741 atgttgctta gagccttgta aattcgctct gaaaaaataa ctcaattcta atagtaactc
    10801 tcgtgccttt tcgctattta ttctaactaa agctgagatc gtgttaattg aattgaagaa
    10861 aaaatgtgga ctcacttgtg cctgtaatga cttaatctca gcatctttca ataacttact
    10921 ttgcgtttcg gcttcaccaa gttcaatttg gctactaaaa atatttgcca atccttctgc
    10981 aagttgacgt tccacaaaag ttaaatcatt agggtttgta aaatacatct tcaatgtacc
    11041 gacgatagaa ccatgcatct caagtggtat cacgatagct gctctaagcg ggcaattcgg
    11101 atgactacaa ccaatctctt ctttagtatg aacttctttc aactttcctg atttcaatac
    11161 atctttagac agacttgtta atatttcatt tgttggtatg tgatgatcac tacctgcacc
    11221 tacatgcgat aagatttcat ttttgcttgt aattgctacg gcagatactt tcattaaatt
    11281 tttaataatc atcgcaattt gctgtgccga ttctctattc aatccttctt taaaatacgg
    11341 caatgtctgg ttcatcaatt gcagtacatc atgtgtttga acagccttca tttgctcctc
    11401 ttgctttaat gttgaaatga taatagacat aaaaatcgcc gtaccaacgc tattaacaat
    11461 aatcattggt agtgcaatta atgatatgag gtcaaccgca tatgctttgt cgtgggaaaa
    11521 tgttaaaatg ctcaacattt gaatcatttc cataacaatt ccaatcatgg cacttttcgc
    11581 aatactcggg taacgcttgc gtctttgagc ttgtaagcca aaataaccag caattatacc
    11641 aataaatata gatgagataa gataaacttg tgcatccgcc ccacccatat acactctgaa
    11701 aatacctgaa ataacgccaa caaatagacc tacaaaaggg ccaccaacta atcctgcgac
    11761 acctatcgtt aatacacgtg tgttagctaa agatacatca tcatctaaac ggaagtacac
    11821 acttcctgac aaactatgtt gatgatcgat gacgatacca gttaaattag acattaaggc
    11881 aaacaaactg aaaataatac ataattgcca acgtgctttc catgtacgtc gacgattcat
    11941 taagttttta aaatatggaa tattcatcaa cacataggcc aaaataataa ttaaacctac
    12001 acgctcaagt aataacattg ttagcgatag cacgatagca cctcagtgaa ttataattat
    12061 gtttatttta tcataatagt gcatgtaaaa ttcatgaata aattattaaa aaaacacgct
    12121 actatttaat tatctatacc attaaatttt catatcaatt tttatgctaa gttgaccatt
    12181 tttcttttca aataatgctg tttatgtcac tattatgact tttatacata gcgaaaaact
    12241 gctggactta tttttgccaa cagtttctaa tcgcgttatt tatgatactt ttttaaataa
    12301 tacgtgattc tctaaatgta cgtgttcatg tgttaacact tcaagtgctt ttaatctctg
    12361 ataaacaagt cgccaagtac cacacgcttc tatcggaggt tcataactag atgttaattc
    12421 gctcattttt actaacaatt ctcccgttgc aatgtggtct gaaactaaat catctatcac
    12481 agtattaata tcgtctacta cctcaccttg ctcatattta atgagttttg gaaaatcgac
    12541 atcgtcttct ttttgcatat gctctaacat gccattttta aatgtatcgt atgtttcttt
    12601 taactcaact aaatatggat gattaggtcc atgtactttc gataacttcg tcacataagg
    12661 tgttaaattt ttaaattctt ctcttagagg ttcatgatat gctgattgaa tatattgaat
    12721 aagtgatgaa acatttaaaa atttaggatt taacgaacct ggtgtattcg tttgttcaac
    12781 gtcgttgaga cgctgtaata attcgttcaa atctacattt ttcttttcta aggctgctgc
    12841 ttctatactt acttgtccgc cacaacaaaa atctattccc acacttctaa aaatatccgc
    12901 tgctttcgga taatcagtta ctacatctgc tactatgtca tttttattta tcattataga
    12961 taacctcctc atttccagtg tatacaattt aatgtatatg aaaatgaaaa tcagggaaat
    13021 gtcataatca tgtcataaac agcaaaataa agccctcaaa tcatgttggc tcacaatgat
    13081 ttgagggcat ttattattta tttagtggat aagtgatatg tgcagtgttt aacttaacaa
    13141 tatttttgaa gtctcgcttt ttaatatctt tgctaagtaa gatttgcact tcgttttctg
    13201 atgtttggct taacattccc atcaccatgt cgtcttttag tacgacacta gccatacctt
    13261 cttcaaatac ataaccacga tccagaccag ttaaaatgat tttgttttta ccttttaact
    13321 tcaatgagat tgaatccgtt aatgtaatag ctgtaaagta taacgaaatg ttattaaagt
    13381 cttttgtgaa atacggtgtt aatctataaa aatcattatt caaatgaatt aatgcacttg
    13441 tttctgcagt atatgcatat ttattacgtt gattaccaac acgcacttca aatacttccg
    13501 tacctatttg gacttctaag aaaacatccc aaattttttc tttaggtaaa tacgctttaa
    13561 tatcttctaa cataatgtta aaacgatatt gtttattcgc attgacactt aaaggtacat
    13621 acattgtgtg cttcttgttt cgtgaatgta atttaagtct gacatcataa tgtccttcgc
    13681 caatcataaa ttcatttaac gttagctctg taacaatttt atgattatca aatttaaatg
    13741 ctgtcatttt cacttttggc ttagcgatat caatgccttt aaagtatggt tcgccttcat
    13801 tgaattgaat gtttaattta ccatctacaa tatcaaaacg gtagtattga ccttgacggt
    13861 aactttcctc cacagttcta tagttgtcta tatctttcgc tctcgcgtag tgcaacaatg
    13921 gtcgtaattt actcatcacc aatgcatcta cacgttctgg aactgcttgt ataaagtctc
    13981 ctagagcatt aatccattgt ggttgatctt ctagtttaac tttaagtgag aagccattcg
    14041 tacgagaaaa actaaaatga cgatttaaaa attctgctaa gatttgattt ttatgggctt
    14101 cttctaaatc tgcatttaat atttctacag caatcaatct catgacttca taaaagtctt
    14161 caggtgaaac atacgcacta ctcatatgtt caccttcacg ctttgtagca taataatacg
    14221 ctttatcact taacacactg attcgatttg catttaaata tgctttcatt gtaaataatt
    14281 gatcttctgc actctttaat tcttctggaa aataaatgtc attatctttt agtaatgctg
    14341 ttctatagat tttagtcggg cttaaagtat agataattct tgaatttaac aaagtaactt
    14401 caggtgctgt ttctttaaac atagattgtg gtacaccacg accattaaca cctttcattt
    14461 taatcaataa gacatctgag tgatgttcat caataaatgc tgctgcatcc tttaaagtct
    14521 ctttgtttat atagtcatcg gagtccacaa ataatacaaa ttcaccttct gcttgtttta
    14581 acgccacatt tctaggttta cctggaccac cagaatttgt ttcgagttga cttactttca
    14641 agttcaattt attgcgatac ttttcaacta tttgtaacgt ttgatctgtt gaacagtcat
    14701 caactacaac cacttcaaat tcagttttcg gaaaatcttg tttcgcaagg ctattaagta
    14761 attctgttat atacttttct gaattgtatg ttggaactat tactgaaaat ttcatcatta
    14821 tacctctccc actttgacta ctatataaac ttagctacca aataaatttc tgactaaacg
    14881 ctcacttgat cggccatctt gatatttaaa atgtttatct aagaatggaa tgactttttc
    14941 tccttcataa tcttcattgt ccaaggcgtc cattaatgcg tcaaatgatt gcacaatttt
    15001 acctggaaca aatgattcat atggttcata aaaatcacgc gtcgtaatat aatcttctaa
    15061 atcaaatgca tagaaaatca ttggcttttt aaatactgca tattcatata ttaaagatga
    15121 atagtcacta attaataaat ctgttatgaa cagtatatca ttaacttctc taaagtcaga
    15181 aacgtcaaca aaatattgtt tatgtttgtc tgcaatatta agtctatttt tcacaaatgg
    15241 atgcatttta aataatacaa ccgcgttatt tttttcgcaa tatcttgcta aacgttcaaa
    15301 atcaattttg aaaaatgggt aatgtgctgt accatgacca ctacctctaa atgttggtgc
    15361 gaaaagaatg actttcttac ctttaataat tggtaattca tcttccatct cttgtttgat
    15421 ctgtgtcgca taagcttcat caaatagtac atcagtacgt ggaacacctg taggcactac
    15481 atttttctct ttaataccaa atgcttcagc gtagaatgga atatcggttt cagatgatac
    15541 ataagctttt gtatagctac gatgatttaa tgaatcaata aatggtccac cctttttacc
    15601 agtacgacta aagccaactg ttttaaaggc accaacggca tgccatactt gaataacttc
    15661 ttgagaacgt ctaaaacgca ctgtataaat caatgggtga aagtcatcaa caaagatgta
    15721 gtctgccttc ccaagtaaat atggcaatct aaacttgtcg atgatgccac gtctatctgt
    15781 aatattcgct ttaaaaacag tgtgaatatc atacttttta tctaaatttt gacgtaacat
    15841 ttcgttatag atgtattcaa agtttccaga catcgttggt ctagagtctg atgtgaacaa
    15901 caccgtattc ccttttttca agtggaaaaa tttcgtcgta ttaaatatcg ctttaaaaat
    15961 aaattgtctt gtattaaatg attgtttgcg gaaatactta cgtaattctt tatatttacg
    16021 aacgatataa atacttttaa cttccggagt cgttacaaca acatcaagga caaattcatt
    16081 aacatcgcta gaaatttcag gtgtaacagt ataaaccgtt ttcttcgaaa tgccgccttt
    16141 tctaaattct tttaggtaag tctgcaataa gaaattgatt ttaccatttt gtgtttctaa
    16201 ttcgttgtat tcttcttctt gttctggctt tagattttga tatgcatcat taatcacatc
    16261 tgggtttaac tgtgcaatat aatcaagttc ttgctcattc actaataagt acttatcttc
    16321 aggtaagtaa taaccattat ctaagatagc tacattgaaa cgacaaacga attgattccc
    16381 atctattttg acatcattcg ccttcattgt acgtgtctca gttaaatttc ttaatacaaa
    16441 attactatct tctaaatcta ggttttcact atgtccttca acgaataact gaacacgttc
    16501 ccaatagatt ttatctatat atatcttact tttaaccaac gttaattcat ccttttctat
    16561 ttacataatc cattttaata ctgttttacc ccaagatgta gacaggtctg cttcaaaagc
    16621 ttctgtaaga tcattaattg ttgcaatttc aaattcttga ccttttaaca acgctaattt
    16681 atctacaata tctgggtatt gaatgtataa gtctacaaca tcttggaaat cttttgaacc
    16741 acttcgacta ctaccaatca acgttagtcc tttttccaat actagacgtg tattaacttc
    16801 tactgggaac tcacttacac ctaacagtgc aatgcttcct tctggtgaaa tgtaatcgat
    16861 catttgattt atggctgatt gactaccgcg accacccaca cactcaaatg catgatcaaa
    16921 tgttaagcct tcaggtattt tattaataaa gaagacatca tcaacaaatg agaagtgact
    16981 caatttataa tctgttttac caaagacata tattttagac tctggatata atttacgtaa
    17041 taaaatggct gtaatgtaac ctaagttacc atcaccccaa ataccaaatg tatttttatt
    17101 tgaaatagat ttcttttcaa aacgacgaat agcatgcaaa cttactgtta caagctctgt
    17161 atatgaaata atacttaaat caatatcatc aggtagtggt acagctctat catgatttag
    17221 caacacaaaa tcttgcataa acccatcatg tccacttgat ctgaagtagc tcgattttaa
    17281 atagttttca gcaatgacat cgtctttttc tgtcggcgta ttcggtacca taactacttt
    17341 tgtaccttta ttaaacacac ctttactgtc aaatacgacc tcaccaacac cttcatgaat
    17401 taaagacatt ggcaatttct gagataagac attctcatca cggctaccag tataatatct
    17461 ttgatcggca gcacaaattg acatatataa aggacgtaca atgacatagt cactgtaaat
    17521 atctacgttg ttatacgtaa cttcaaattg tctaggtgca actaattgat atacttgatt
    17581 aatcatcggc aataccacct cgaataatag cattcgctac ttttaaatcg taaggtgttg
    17641 ttactttaat gttatataac tcacctttta caagtcgaac cggtttgttt gtttctacaa
    17701 taatcttaca agcatcagat aaaatactct tttgctcatc actcaactgt gcatagcttt
    17761 cttttaataa attaatatta aacgattgag gtgtttgacc ttggtacatt tcattacgca
    17821 ctggaattgc atcaatcgtt tgattatctt tagatgtaac aatcgtatct atagcatcaa
    17881 tcactgtatc tactgcaccg tactctaaag cagcttgaat attttcttta ataatacgat
    17941 gcgttaaaaa tggtctaact gcatcatgtg tcacaatgac atcgtcatcg ttaataccat
    18001 ttgttgattc aatatgttta acgatattca taattgtatc gttacgatcg ctaccacctt
    18061 gaatgacttc aattctttca tcagaaattt tgaattttct aagtgtatct ttcgtatgcg
    18121 tcatccattg ttgtggcgtc gcgataataa ttttttcaaa atcattaatt aaaataaatt
    18181 tttctaatgt atggattaaa atcggtttgt tgtctaaatc taaaaattgt ttaggtaaag
    18241 gtacgttacc cattcttgag cctatacctc cagctagaat accagcgtat ttcataaaat
    18301 ac
//
LOCUS       DAMQYT010000028        18285 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMQYT010000028 DAMQYT010000000
VERSION     DAMQYT010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18285
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.028"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            54..812
                     /gene="nagB"
                     /locus_tag="PGA33_000798"
     CDS             54..812
                     /gene="nagB"
                     /locus_tag="PGA33_000798"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDG6472629.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            889..1521
                     /gene="hxlA"
                     /locus_tag="PGA33_000799"
     CDS             889..1521
                     /gene="hxlA"
                     /locus_tag="PGA33_000799"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HDG6472630.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIIDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKDIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            1523..2071
                     /gene="hxlB"
                     /locus_tag="PGA33_000800"
     CDS             1523..2071
                     /gene="hxlB"
                     /locus_tag="PGA33_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HDG6472631.1"
                     /translation="MAKFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            2184..2831
                     /locus_tag="PGA33_000801"
     CDS             2184..2831
                     /locus_tag="PGA33_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDG6472632.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQRAGMQSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            3333..4733
                     /locus_tag="PGA33_000802"
     CDS             3333..4733
                     /locus_tag="PGA33_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDG6472633.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
     gene            5277..6653
                     /locus_tag="PGA33_000803"
     CDS             5277..6653
                     /locus_tag="PGA33_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDG6472634.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            6655..7794
                     /locus_tag="PGA33_000804"
     CDS             6655..7794
                     /locus_tag="PGA33_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDG6472635.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            7769..8134
                     /locus_tag="PGA33_000805"
     CDS             7769..8134
                     /locus_tag="PGA33_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HDG6472636.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            8137..8406
                     /locus_tag="PGA33_000806"
     CDS             8137..8406
                     /locus_tag="PGA33_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472637.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(8607..8774)
                     /locus_tag="PGA33_000807"
     CDS             complement(8607..8774)
                     /locus_tag="PGA33_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HDG6472638.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
     gene            complement(9281..10111)
                     /gene="thiD"
                     /locus_tag="PGA33_000808"
     CDS             complement(9281..10111)
                     /gene="thiD"
                     /locus_tag="PGA33_000808"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDG6472639.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
     gene            10295..10951
                     /locus_tag="PGA33_000809"
     CDS             10295..10951
                     /locus_tag="PGA33_000809"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDG6472640.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA
                     "
     gene            10952..11332
                     /locus_tag="PGA33_000810"
     CDS             10952..11332
                     /locus_tag="PGA33_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HDG6472641.1"
                     /translation="MLNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTVAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            11465..11833
                     /locus_tag="PGA33_000811"
     CDS             11465..11833
                     /locus_tag="PGA33_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDG6472642.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            11954..13438
                     /locus_tag="PGA33_000812"
     CDS             11954..13438
                     /locus_tag="PGA33_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDG6472643.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(13580..14032)
                     /locus_tag="PGA33_000813"
     CDS             complement(13580..14032)
                     /locus_tag="PGA33_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDG6472644.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(14045..14809)
                     /locus_tag="PGA33_000814"
     CDS             complement(14045..14809)
                     /locus_tag="PGA33_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDG6472645.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            complement(15358..16110)
                     /locus_tag="PGA33_000815"
     CDS             complement(15358..16110)
                     /locus_tag="PGA33_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HDG6472646.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRIVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHIINTNEFDQFFAIS"
     gene            16278..17264
                     /gene="pta"
                     /locus_tag="PGA33_000816"
     CDS             16278..17264
                     /gene="pta"
                     /locus_tag="PGA33_000816"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDG6472647.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            17267..18103
                     /locus_tag="PGA33_000817"
     CDS             17267..18103
                     /locus_tag="PGA33_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDG6472648.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFENENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
BASE COUNT         6320 a         2740 c         3349 g         5876 t
ORIGIN      
        1 gaatgaattc ataagttttt aaaatgtatt aaatttgtgg aggcatgtaa acaatgaaag
       61 tattaaactt aggatcgaaa aaacaagcat cattctatgt tgcatgtgag ttatataaag
      121 agatggcatt taatcagcac tgtaaactag gtttagcaac tggtggtaca atgacagatt
      181 tgtatgaaca acttgttaag ttattaaata aaaatcagtt aaacgtagac aatgtatcca
      241 cgtttaattt agacgaatat gtaggtttaa ccgcatcaca tccgcaaagt tatcactatt
      301 atatggatga catgcttttc aaacaatatc cttattttaa tagaaagaac attcatattc
      361 caaatggaga tgccgatgat atgaatgcgg aagcgtcaaa atataatgac gttttagaac
      421 aacaaggtca acgtgatatt caaattttag gtattggtga aaatggtcat attggattta
      481 atgaacctgg tacgccgttt gatagcgtta ctcatatcgt tgatttgact gaaagtacta
      541 ttaaggctaa tagtcgatat tttaaaaacg aagatgatgt tccaaagcaa gccatttcga
      601 tgggacttgc taatattctt caagccaaac gtatcatttt actcgcattt ggtgaaaaga
      661 aacgtgctgc tattacacat ttattaaatc aggaaatttc tgttgatgtt ccagccacat
      721 tacttcacaa acacccgaat gttgagatat atttagacga cgaagcttgc ccgaaaaatg
      781 ttgcgaaaat tcatgtcgat gaaatggatt gattgcaatg tttaattaag aaatgcctcg
      841 ggaaaggttc caatagaaag ataaaaagca ttggaaggat gatttttagt ggaattacaa
      901 ttagcaattg atttattaaa caaagaagac gcggctgagt tagcaaataa agtaaaagat
      961 tatgtagata tcgtagaaat cggtacgcca atcatttaca acgaaggttt accagcagtt
     1021 aaacatatgg cagacaacat tagtaatgta aaagtattag cagacatgaa aattatagat
     1081 gcagctgatt atgaagttag ccaagcaatt aaatttggcg cggatgtaat tacaatacta
     1141 ggtgttgcag aagatgcatc aattaaagca gctattgaag aagctcataa aaataataaa
     1201 caattactag ttgatatgat tgctgttcaa gatttagaaa aacgtgcaaa agaactagat
     1261 gaaatgggtg ctgattatat tgcagtacac actggttatg atttacaagc agaagggcaa
     1321 tcaccattag aaagtttaag aaccgttaaa tctgttatta aaaattctaa agttgcagta
     1381 gcaggtggaa ttaaaccaga tacaattaaa gatattgtcg ctgaaagtcc tgatcttgtt
     1441 attgttggtg gcggaatcgc aaatgcagat gatccagtag aagctgcaaa acaatgtcgc
     1501 gctgcaatcg aaggtaagta atatggctaa atttagtgac tatcaattaa ttctagatga
     1561 attaaagatg actttgtcac atgttgaagc ggatgagttt tcaacttttg catccaaaat
     1621 actacatgct gaacatatat ttgtagctgg caaaggacgt tcaggattcg tggcgaatag
     1681 ttttgcaatg cgcttaaatc agctcggcaa acaggcacat gttgttggag aatcaacgac
     1741 acctgcgatt aagtcgaatg atgtatttgt aattatctct ggttcaggtt ccacggaaca
     1801 tttaagatta ttagcagaca aagcaaaatc agtaggtgct gacatcgtat taattactac
     1861 aaataaagat tctgcaatag gcaatctagc tgggacgaac atcgttttgc ctgcaggtac
     1921 aaaatatgat gaacaaggct cggcacaacc attaggaagt ttgtttgaac aagcatctca
     1981 attattttta gatagtgttg taatgggatt gatgactgaa atgaatgtta cggaacaaac
     2041 gatgcaacaa aatcatgcta atttagaata aaataaagat agtcgataat atgatgccta
     2101 ggcagaaata ttatcgatta tttttttatt taaataataa attatagtat aatatcaata
     2161 ataaacgaat aggggtgtta atattgaagt ttgacaatta tatttttgat tttgatggta
     2221 cgttggcaga cacgaaaaaa tgtggtgaag tagcaacaca aagtgcattt aaagcatgtg
     2281 gcttaacgga accatcatct aaagaaataa cgcattatat gggaatacct attgaagaat
     2341 catttttaaa attagcagac cgaccattag atgaagcagc attagcaaag ttaatcgata
     2401 catttagaca tacatatcaa tctattgaaa aggactatat ttatgaattt gcgggtataa
     2461 ctgaagccat tacaagtttg tataaccaag ggaaaaaact tttcgtggtg tctagtaaga
     2521 agagtgatgt attagaaaga aatttatcgg ctattggatt aaatcacttg attaccgaag
     2581 ctgttggatc cgatcaagta agtgcatata aaccaaatcc tgaaggcata cacacaattg
     2641 tgcaacgcta caatttaaat agccaacaaa cggtgtatat tggtgattca acgtttgatg
     2701 ttgagatggc acaacgtgct ggtatgcaat ctgcagctgt cacttggggt gcacatgatg
     2761 caaggtcatt acttcattca aatccggatt ttattattaa tgatccatca gaaattaata
     2821 ccgtattata aaacttgtta aaacagagaa taccatggtt aaaatgcata ttcataaata
     2881 ttagattata cttagaaata tttcgcttta gattaggaat ttaaaataaa tatttattaa
     2941 acattatgaa tttttaaaga gtaatgtctg actcgttgat aatttatttt tgtaaaaata
     3001 aattaaagta atgacaaagt tattgaagta aattgagtat aaacatttaa atacgatgtc
     3061 gaaaatggcg atagcatatc acttacatga agttatgtgc tatcgctatt tttagttata
     3121 attccaaaaa gttaatcgtt cgatgattta agaattatta ttgtttaatt caaatgtatg
     3181 agggtataaa atcattgaat ttaattcgat aaagcgaaat ttttgaacaa acatactttt
     3241 gtatttatat aaaagtttaa attcttataa atttgacaaa actaattaac tccgtataat
     3301 tatgaaacat acaagaggga gtgtatgaat tcatggattt taataaagag aatattaaca
     3361 tggtggatgc aaagaaagct aaaaaaaccg ttgttgcaac cggtatcggt aatgcaatgg
     3421 aatggttcga ttttggtgtc tatgcatata caactgcgta cattggagcg aacttcttct
     3481 ctccagtaga gaatgcagac attcgacaaa tgttgacttt cgcagcatta gccattgcgt
     3541 ttttattaag accaattggt ggtgtcgtat ttggtattat tggtgacaaa tatggacgta
     3601 aagttgtatt aacatctaca attattttaa tggcattttc aacattaacc attggattat
     3661 tgccaagcta tgatcaaatt ggactttggg caccaatact attattgctt gcaagagtac
     3721 tacaagggtt ttcaacaggt ggagagtatg cgggggcaat gacatatgtt gccgaatcat
     3781 ctccagataa gcgtcgtaac tcattaggta gtggactaga aattgggaca ttatcaggtt
     3841 acatagctgc ttcaattatg attgctgtat taacattctt tttaacagat gaacaaatgg
     3901 catcatttgg ttggagaatc ccattcttac tcggtttatt cctaggatta ttcggcttat
     3961 atttacgtcg taagctggaa gaatcaccag ttttcgaaaa tgatgttgca acacaaccag
     4021 aaagagataa cattaacttt ttacaaatca tcagatttta ttacaaagat atatttgtat
     4081 gttttgtagc tgttgtattc ttcaatgtta caaactatat ggtaactgca tatttaccaa
     4141 cctatttaga acaagttatt aaattagatg caacgacaac aagtgtatta attacttgtg
     4201 tcatggcaat aatgattcca ttagcattaa tgtttggtaa gttagcggat aaaataggtg
     4261 aaaagaaagt atttctaatt ggtactggtg ggctaacatt attcagtatc atcgcattta
     4321 tgttattaca ttcacaatca tttgttgtaa tagtaatcgg tatatttata ttaggatttt
     4381 tcttatcaac ttacgaagcg acaatgccag ggtcgttacc aacgatgttt tacagtcata
     4441 taagatatcg aactttatca gtaacattta atatctctgt ttcgatattt ggtggtacga
     4501 cgccattagt tgcaacatgg ttagttacga aaactggaga tccattagca cctgcgtatt
     4561 atttaacagc aatcagtgtt attggctttt tagttattac attcttacat ttaagtacag
     4621 caggaaaatc tctaaaaggt tcgtatccaa atgtagataa cgagcaagat agagcttatt
     4681 atgcagaaca tccaaaagaa gcattatggt gggttaaaga acgtaagaat tagagatttt
     4741 aataaaaagt ataaatcaat cgtatataag cactttaaag ctagtaggtt ctgctaactt
     4801 taaagtgctt tttaaattga gaactgtaat tagccgtaat aaagtttttg tatatacata
     4861 aacccccact gcaatgatta tcgcaatggg ggaaagaggg gacttaaagc atatgtttag
     4921 ctttgaatac ttaaaattct cttgctattg aaatgttagg atgtaaatat gtcttagagt
     4981 attttgtcca acgcaattaa tattgagact ctaaccttca atattattat agagaacaca
     5041 aacttaaata gattgggtga cttatttgtg tcagttattg cgattgcgat aacttctttt
     5101 ctctatatac atatagtaac gtcttatcta ataaaaaaca tggtactaca gtatcaaatt
     5161 tatctagggc ttaagtttga tttttataat aggcaggttt acctgataaa aatacttatt
     5221 cattatataa tgttaacaat atgtatttta aagtttacat tgagtgaggg atattgatga
     5281 acgtaatttt agaacagttg aaaacacata ctcaaaataa acctaatgac atagcattac
     5341 atatcgatga tgaaacaatt acatatagtc aactaaatgc ccgcatcact agcgcagttg
     5401 aatctttgca gaaatattca cttaaccctg tcgttgctat taatatgaaa tcaccggtgc
     5461 aaagtattat ttgttattta gctttgcatc gtttacataa agtgcctatg atgatggaag
     5521 gtaaatggca aagtactata catcgtcaat tgattgaaaa atatggtatt aaagatgtaa
     5581 ttggagatac aggtctcatg cagaatatag actcaccgat gtttattgat tcaacgcaat
     5641 tacagcacta ccccaattta ttacatattg gttttacttc agggacaact ggactgccaa
     5701 aagcatatta tcgtgatgaa gattcatggt tggcttcttt tgaagttaat gaaatgttga
     5761 tgttaaaaaa tgaaaatgca atagcagccc ctggaccact atcgcactcg ttaacattat
     5821 atgcgttatt gtttgcttta agttccggtc gtacttttat aggacagacc acttttcatc
     5881 ctgaaaagtt acttaatcaa tgtcataaaa tatcatcata caaagttgct atgtttcttg
     5941 ttccaacgat gattaaatca ttattgttag tttacaacaa tgaacataca atccaatcat
     6001 tttttagcag tggagataag ctgcattctt ctatttttaa aaagataaaa aatcaagcaa
     6061 atgacataaa tttgattgaa ttttttggta catcggaaac cagttttatc agctataact
     6121 tgaatcagca agcaccagtt gaatcagtag gtgtgctatt tccaaatgtg gaattgaaaa
     6181 caacgaatca cgatcacaat ggtataggaa ctatttgtat aaaaagtaat atgatgttta
     6241 gtggctatgt aagtgaacaa tgtataaata atgatgaatg gtttgttact aatgataatg
     6301 gctatgtaaa agagcagtat ttatatttaa cgggacgtca acaggatatg ttaattattg
     6361 gtggtcaaaa tatatatcca gcacatgttg aacgcctttt aacgcaatct tcgagcattg
     6421 atgaagcaat tatcatcggt attccaaatg agcgttttgg tcaaataggc gtattgcttt
     6481 attctggtga tgtgacactt acacataaaa atgtaaaaca atttttaaaa aagaaagtga
     6541 aacgctatga aattccatcg atgattcatc atgtagaaaa gatgtattac actgcaagtg
     6601 gtaaaattgc tagagaaaaa atgatgtcga tgtatttgag aggtgaatta taatatgaat
     6661 caagcagtca tagttgcagc taaacgaact gcatttggga aatatggtgg cactttaaaa
     6721 catttagagc cagaacaatt gcttaaacct ttattccaac attttaaaga gaagtatcca
     6781 gaggtaatat ctaaaataga tgatgtagtt ttaggtaatg ttgttgggaa tggtggcaat
     6841 attgcaagaa aagcattgct tgaagcgggg cttaaagatt caatacctgg cgtcacaatc
     6901 gatcggcaat gtgggtctgg acttgaaagt gttcaatatg catgtcgcat gatccaagcc
     6961 ggagctggca aggtatatat tgcaggtggt gttgaaagta caagtcgagc accttggaaa
     7021 atcaaacgac cgcattctgt gtacgaaaca gcattacctg agttttatga gcgtgcatca
     7081 tttgcacctg aaatgagcga cccatcaatg attcaaggtg ctgaaaatgt ggccaagatg
     7141 tatgatgttt caagagaatt acaagatgaa tttgcttatc gaagtcatca attgacagcg
     7201 gaaaatgtaa aaaatggaaa tatttctcag gaaatattac ctataaccgt taaaggagaa
     7261 atattcaaca ctgatgaaag tctaaaatca catattccga aagataactt tggccgattt
     7321 aagcccgtga tcaaaggtgg gaccgttacc gctgcgaata gttgtatgaa aaatgatggt
     7381 gcagttttat tgcttattat ggaaaaagat atggcatacg aattaggttt cgagcatggt
     7441 ttattattta aagatggtgt tacggtaggt gttgattcta attttcctgg cattggtcca
     7501 gtaccagcca tttccaactt actaaaaaga aatcaattaa cgatagaaaa tattgaagtc
     7561 attgaaatta acgaagcgtt cagtgcacag gtagttgcct gccaacaagc tttaaatatt
     7621 tcaaatacgc aattaaatat atggggtggt gcattagcat caggtcatcc atacggtgca
     7681 agcggtgccc aattagtgac tcgattattt tatatgtttg acaaagagac tatgattgca
     7741 tctatgggga tagggggagg tctaggaaat gcagcattat ttactcgatt ctaaccagcg
     7801 attaaatgtg tcattttcta aggatagtgt ggctgcatat tatcagtgtt ttaaccaacc
     7861 ttatagaaaa gaagtaccac cattaatgtg tgcgtcatta tggccaaaat ttgatttatt
     7921 taaaaaatat gcaaatagcg aactgatttt aacaaaatca gcaattaatc aaactcaaaa
     7981 gatagaagta gacacaatat atgtagggca tttagaagat attgaatgcc gacagactcg
     8041 caatatcaca cgttatacaa tggctttaac attaactaaa aatgatcaac atgtcataac
     8101 ggttacacaa acttttatta aggcgatgaa gtagagatga agtttaatga gatatggata
     8161 aatgaatatt tggcgctcgt aaatgatgat aatccaatac ataatgagat tgtgccagga
     8221 caattagtga gtcaaatgat gctgatggct atgtcattag agacaaacca gtgtcaaatt
     8281 aactacgtta aacctatttt aataaatgaa aatatcgaat tcattgaaca acacgaacac
     8341 gaaattatag caattaatga cgatggagag attaaaataa aaatttcttt gagcacaaaa
     8401 aaataaccga tattagctgc atgaacgcat attaattagg agatgaaagg acagctaata
     8461 tcagttatgt attgttatta ttattgggaa cagagatgaa tataggttac gtttctttct
     8521 ttgcacgggg atgcattaat ctaaaataat aataacaact atatcaatgt ttaataaatt
     8581 ctggattatt ggaacgatta gtcaatttaa ctaactttca tatgatctat atcgtcttgt
     8641 aataaagaga gcaatttgaa tatttcagta tcactaaatg aatcgtcaca tttaattgaa
     8701 acatgctgaa acgttttggt tataatttca taaactggtg cgccttcatg gtgatactgt
     8761 cgataaataa tcataaccta tattacctcc tttgctactc tatggttata ttataaataa
     8821 catttttatg tgtgacatca accttaagta tcaacttttt atcagacata gaacgtatga
     8881 tttactaaga ctatttatgt ataaaagttc taaataaata tatatttata gagtcgcctg
     8941 gcagtcattt gggaaatata acatatatga ttagagaggc atctatcgca aaagaatgat
     9001 aatgatagag gtattgagca tatagatgag tttaagttca tcttgaaaat aaagggttat
     9061 ttagtcatag atgtagatgt ataggaaata tttgtatgta ttgttcgata tgtatgaaat
     9121 tttcaataaa agctaataac gcttatatgt aactttcaaa tttaaattat atacagagca
     9181 tgatgattat aaaaaaataa ccacatcaca taaattgagt tcatacccaa tttaagtggt
     9241 gtggctaata atgttgattt atagatgaac cgcctaatcg ttaaacctct gttacttcaa
     9301 catcgatatg ttcaatacgg ttgtatgcac cgtgatccac aggaccaaca aaatcattca
     9361 ttttccaacc gtttttaata gcagaagcga cgaaagcttt cgcgctaatc acagcttctt
     9421 tcggtgactt accgttagct aaatatgcag ttgttgccgc agcaaatgta caaccagcac
     9481 catggttata actttgttgg aacatgtctg ttgttagttg ataaaatgtt tgaccatcat
     9541 agtataagtc atacgattta tcttgatcta aagctttgcc acctttaatg atgacatgct
     9601 gtgcgccttt atcaaagata attgttgcag cctttttcat atcttcaatt gaatttaatt
     9661 tacctaatcc tgataattga cccgcttcaa ataagtttgg tgtcactacc gttgctttag
     9721 gtagtaaata tttaatcatc gcctcagtat ttccaggatt aagcacttca tcttcgcctt
     9781 tacaaaccat gacaggatct actacaaaat attgtgcatt agatgcctca tatacttctc
     9841 cagcacgttt gattatctcc tcagtaccta acatacctgt tttaatagca tcaggtccga
     9901 ttgataaagc cgtttcaagt tgtttttcaa atacatccat tggtaatggt gtaacatcgt
     9961 gtgaccatgt atctttatcc atagtaacga tggcagttaa agcgaccatg ccatacgtat
    10021 ctaattcttg gaacgttttc aaatctgctt gcatacctgc gccagcactt gtgtcagaac
    10081 cggcaattgt taaaactttc tttaaagcca ttgagcttca ctcctacata ataatattgt
    10141 attcatcata tcatttttaa cctaattgaa aaatattaag cattcaatat ttgatgattg
    10201 ttgaaatgaa tcattcatac tattgtaact tttgaaaatg tcattcactt tagataagtg
    10261 tgatatgtta aaatatgtcc tgaggtgaga ttgaatggaa tggtcgcaaa tttttcatga
    10321 cataacaacg aaacatgact ttaaagctat gcatgatttt ttagaaaaag aatattcgac
    10381 tgcaatcgta taccctgata gggaaaatat atatcaagcg tttgatttaa caccgtttga
    10441 aaatatcaaa gttgttatat taggacaaga cccgtatcat ggtccaaacc aagcacatgg
    10501 attagcattt tcagtgcaac ctaacgcaaa attccctcca tctttacgta atatgtataa
    10561 agaattagca gatgatattg gatgcgttag acaaacaccg catttacaag attgggcaag
    10621 agaaggcgtc ttgttattga atacagtttt aaccgtaaga cagggtgaag caaattctca
    10681 tcgtgatatt ggttgggaaa catttactga tgaaattatt aaagcagtgt ctgattataa
    10741 agaacatgtt gtctttattt tgtgggggaa acctgcacag caaaaaataa agcttatcga
    10801 tacatctaaa cattgtatta taaaatcagt gcatcctagt ccactgtctg catatagagg
    10861 attctttgga tcaaaaccgt attccaaagc gaatgcctat ttagagtcag taggaaaatc
    10921 accaattaat tggtgtgaaa gtgaggcgta gatgttgaat agagaaactt taatagcacg
    10981 aattgagcaa gaattagtac aagcagagca ggcacagcat gaccatgact ttgaaaaaca
    11041 tatgtatgcc atacatatat taacatcttt atatgcttca acatcaaata caccacatat
    11101 tggtgaacaa caaatgaatc gtcgtattgc taaccataat caaatgccac aatcacaaat
    11161 aacgcagcca actcatcaag tgacagttgc tgaaattgaa gcgatgggtg gtaaagtaaa
    11221 tacgcattca gcacatcatc ataataagtc atattcacaa ccttcaaacc aacaacaaag
    11281 attagcgaca gatgatgaca ttggcaatgg tgaatccata tttgattttt aaaaagcaac
    11341 aatgaaacat aattacttaa tagcttgtta agtatgtagg ttaataatca agacgcatat
    11401 acttttattc gagtgttcgg atttaaacat ttattaatac tgaattatat aaggagaggt
    11461 agcaatgaaa ttatttatta ttttaggtgc attaaacgcg atgatggctg tcggtacagg
    11521 tgcatttggt gcgcatggtt tacaaggaaa aataagtgat cactatttat cagtatggga
    11581 aaaagcaacg acgtatcaaa tgtaccatgg cttagcatta ttaattatag gtgtaattag
    11641 tggtacaact tcaatcaatg ttaactgggc tggctggtta atatttgctg gtattatttt
    11701 ctttagtgga tcattatata ttttagtatt aactcaaatt aaagttttag gtgcgattac
    11761 gccaattggt ggcgtattgt tcatcattgg atggataatg ttaatcattg cgacattcaa
    11821 atttgctggt taaattttaa aactttagat tacctatgta actaaacatt aaatttttaa
    11881 taaaaataat caagaaaaag agttacaaac tcatcttttg ggtatagaat accttcgagg
    11941 tgagttttta tttatggaaa aaaagaataa gcaaatagat agaggcgatt taaaacaaaa
    12001 cctatctgaa aagtttgtat gggcgattgc atatggttca tgtatcggat ggggcgcatt
    12061 catcttacca ggagactgga ttaagcagtc aggtccgatt gcagcatcaa ttggtatagt
    12121 tattggtgca ttattaatga tattaattgc ggttagttat ggcgcattag tagagagatt
    12181 tccagtatca gggggcgcgt ttgcctttag tttcttaagt ttcggcagat atgtgagttt
    12241 cttctcatca tggtttttaa cttttggtta tgtctgtgtc gttgctttaa atgcgaccgc
    12301 attcagttta ctagttaaat tcttattgcc agatgtctta aataatggga aactatacac
    12361 cattgcgggc tgggacgttt atattacgga aatcattatt gcgaccgtat tactacttgt
    12421 attcatgcta gtaacgattc gtggcgcaag tgtatctgga tcattacaat attatttctg
    12481 tgtggcgatg gtaatcgtcg tattattgat gttctttggt tcattctttg gtaataattt
    12541 tgcacttgaa aatttacaac cgttagctga acctagcaaa ggatggttag tgtctattgt
    12601 ggttattgta tccgtggcac catgggcata tgttggattt gataatattc cacaaacagc
    12661 agaagagttt aactttgcac caaacaagac atttaagctt atcgtgtaca gtttattagc
    12721 agcatcatta acttatgttg tcatgatttt atacactggt tggttatcaa caagtcatca
    12781 aagtttaaat gggcagttgt ggttaacagg tgctgttaca caaacagcat ttggttatat
    12841 tggattaggt gtattagcaa ttgcaattat gatgggtata tttactggtt taaatggatt
    12901 cttgatgagt tcaagtcgct tgttattttc tatgggacgt tcaggtatta tgccaacaat
    12961 gtttagtaaa ttacatagta aatacaaaac accatatgtc gcaatcatat tcctagtagg
    13021 agtgtcgtta attgcacctt ggctaggaag aactgcattg acttggattg tagatatgtc
    13081 atctactggt gtatccattg cctactttat tacatgtttg tctgcagcga aattattcag
    13141 ttataacaaa caaagtaata cgtatgcacc ggtttacaaa acgtttgcta ttatcggctc
    13201 atttgtatca ttcattttct tagcgttgtt attagtgcca ggttctcctg cagcactgac
    13261 tgcaccgtct tatattgcat tacttggatg gttaatcatc ggtttaatat tctttgtgat
    13321 tcgatatcct aaattgaaaa atatggataa tgatgaatta agtcgcttga ttttaaatag
    13381 aagtgaaaat gaagttgatg atatgattga agaacctgaa aaagaaaaaa ctaaataata
    13441 aaagaatcgc acaataaacc ttcttcattc ggaggcgtat cgtgcgattt tttgtattat
    13501 aaattgacat ttaagacgag gcagctgaac cttatatata attgctaaga gttagggctg
    13561 agccatttct aacaaatatt tataatcgtt taaaagattt cacgaaccca gaaacaatta
    13621 atttggaaat ttggtcggcg aataataaac ctaatgcgat ggcgcctgca ataagtgtaa
    13681 cctctagcat ggtattgatt gctgtactga aatttaataa gactaaattt tttgtagcat
    13741 cgtatgctaa gccaccaggt actaatggaa tgatacccgt taccataaaa atgatggcag
    13801 gttctttttg tttacgagcc atataatgac ttaacaagcc taatgctaaa ctaccaaaga
    13861 aactagagta tatagtgtgc acattaaagc cgttgaagaa taaggtgtaa accatccatc
    13921 cacacgtacc aacgaaacca catgatagat ataattttct aggtgcatca aaaatgacgc
    13981 agaagaacat tgaagctaaa aagctaaaga taaagtttaa gatccaaaac atagtctgat
    14041 actcctatac taaaattaat acgctaccaa cgccagcacc gatgccaaac gcagtaacca
    14101 atgcttctaa tgatttcgtt gtgaacatca acatgtgtcc accaaataaa tcttgtattg
    14161 cgtttgttat taatacacca ggaacaatag gcatgactgc cgcaatgata atagttgcca
    14221 agtcacctgt tggaataagt gtatgtccaa taacggcgat aatcccaata actaatgaac
    14281 caatgaattc tgggataaac tgtgcgtgta acttacgatc taaaatctca gtgactaggt
    14341 atcctagact acctgctaat atcgcagtta aaacatcaat caatctacca ccttgtaaat
    14401 ataagaaact cattgcaatc attgctgcag caaaaccttt aaagggaaga ctgctgtcac
    14461 gcttagcaac atatattttt tcaagttgcg tttttgcttc ggctaaagaa atttcattgt
    14521 ttgtaatttg acgcgaaatt ttattagctt gagaaatttt tattaagttt gtatctcgag
    14581 aggtaattct aaatattcta ggaaacgatt ccgaatgtaa cgtaaactgg atgacagtgt
    14641 ttgtaacaaa gctgttactt tcactgtaac caagtttttt tgcaatacgt gtcatggtat
    14701 cttctacacg cgtaccttct gcaccagatt ctaatagtat gcgagcagca agcatgacaa
    14761 cgtctttgat aagtacctct tgtttgtatt cttctgaatt tatgtccatt tcatcaccat
    14821 tgtttataag aatttaatac tcattatagt ttatacacta taaaataacc acatgagctt
    14881 tttgataagt tgttattgat tttaaaaaac taatatttga tactattgta ataataatca
    14941 tcggaatttt tagtagttaa aattctcttg aagtataaaa aggaaaaatt attgaaaaaa
    15001 attcgacaaa aatatgtttc aaatatttca aataggttaa aataatatta gttaaccatt
    15061 acaaaaattg tatagagtag cgactgtata atttctattg aggttaacgt ttatatgtag
    15121 tgatagtagt taaagttctc ccaaggaaga ctactcgggt acactttgct atgagcaaag
    15181 tgtactttgt tattgataat acattagcac atatatataa gtttaaacat acagatttca
    15241 actatttacc aaatcacgtc ttatgtatac gggaatatac tgaagataaa cgaaaaattc
    15301 caagcttaaa ccgataagct tggaattttt ttattaattt ataaacgtac caatgtatta
    15361 agaaatcgca aagaattgat cgaattcgtt tgtgttaata atatgtccta caaagaaact
    15421 accgaattca ccgtatcgtg ctgttgtttc atcaaagcgc atttcgtata caattttttt
    15481 gaattgtaat acgtcatctg agaacaatgt tacgccccat tcgaaatcat caaaccctac
    15541 agaaccagta ataaattgtt tgattttgcc agcatatttt ctaccaatca taccatggtc
    15601 atacattaat ttttggcgtt cttccatagt taacatgtac cagttataag tttcattacg
    15661 acgtttgttc attggataga aacaaatata atcagaatgt ggtaattctg ggtataatct
    15721 tgctttgata tgagggttct cataaggatc ttcatcagat ttaccagcta aataattgct
    15781 caattcaatg actgatacat atgaatatgt agggattagg aagtcagcaa tgcgcaattt
    15841 gttaaattca ttttcaatat gatttaaaga cttcatttca ggacgtaaga accataataa
    15901 caaatctgct ttttgaccag ttatattata aatagcttga tcaccagatt ttgatgatct
    15961 tacagttgct gtattttcta aaaatgattg aaattcagtg acaagtgcat cgcgttcgtc
    16021 ctttggaact atacgtaatg atgcccaatc aactgcataa aataaatgta gactatacca
    16081 accatctaat gtttcggctg cttgactcat gtttatcgct ccttttcaaa aatcatcagt
    16141 tctttataaa ttttatcata aagcaaaggg gtaacgataa aattatgatt acaaattggt
    16201 gacgtggcat tatgaaataa aatggcgtat aattataccg tgaatgatta ataagattta
    16261 tattacagga ggacattatg gctgatttat taaatgtatt aaaagacaaa ctttctggta
    16321 aaaacgttaa aatcgtatta cctgaaggag aggacgaacg tgttctaaca gctgcaacac
    16381 aattacaagc aacagattat gttacaccaa tcgtgttagg tgatgagact aaggttcaat
    16441 ctttagcgca aaaacttgat cttgatattt ctaatattga attaattaat cctgcgacaa
    16501 gtgaattgaa agctgaatta gttcaatcat ttgttgaacg acgtaaaggt aaagcgactg
    16561 aagaacaagc acaagaatta ttaaacaatg tgaactactt cggtacaatg cttgtttatg
    16621 ctggtaaagc agatggttta gttagtggtg cagcacattc aacaggcgac actgtgcgtc
    16681 cagctttaca aatcatcaaa acgaaaccag gtgtatcaag aacatcaggt atcttcttta
    16741 tgattaaagg tgatgaacaa tacatctttg gtgattgtgc aatcaatcca gaacttgatt
    16801 cacaaggact tgcagaaatt gcagtagaaa gtgcaaaatc agcattaagc tttggcatgg
    16861 atccaaaagt tgcaatgtta agcttttcaa caaaagggtc tgctaaatca gacgacgtga
    16921 caaaagttca agaagctgtc aaattagcac aacaaaaagc tgaagaagaa aaattagaag
    16981 caatcattga tggcgaattc caatttgatg ctgcgattgt accaggtgtt gctgagaaaa
    17041 aagcgccagg tgctaaatta caaggtgatg caaatgtctt tgtattccca agtttagaag
    17101 ctggtaatat tggttacaaa attgcacaac gtttaggtgg atatgatgca gttggtccag
    17161 tattacaagg tttaaattct ccagtaaatg acttatcacg tggctgctca attgaagatg
    17221 tatacaatct ttcaattatt acagcagcgc aagccttaca ataacgatgg atttagcgag
    17281 taaatatttt aatggcgtca actggcgata tatcgatcat tcttctggat tagaacctat
    17341 gcaatctttc gcattcgatg atacattttg cgaaagtgtg ggcaaagata tatcagataa
    17401 tgttgtgcgt acttggattc atcaacatac tgttattctt ggtattcatg attcaagatt
    17461 gccgttttta aaagatggca ttgattattt aacgaatgag attggttata atgccattgt
    17521 tagaaattct ggtggcttag gtgtcgttct agatcaaggt gtattaaata tatcgctgat
    17581 gttcaaagga caaacagaaa caacgattga tgaagcgttt actgtgatgt acctcttaat
    17641 tagcaaaatg ttcgaaaatg agaatgttga tattgatacg atggaaattg aacattctta
    17701 ttgcccagga aaatttgact taagtatcga tggtaagaaa tttgcaggca tatcgcaacg
    17761 aagagttaga ggcggtattg ctgtacaaat ttatctttgt gttgaaggct ctggttcaga
    17821 acgtgcattg atgatgcaaa cattttatga acatgcttta aaaggtgaag tgactaaatt
    17881 taaatatcct gaaattgaac catcttgtat ggcctcatta gagacattgc ttaacaaaac
    17941 gattactgtt caagatgtaa tgtttttact attatatgca atcaaagatc ttggcggtgt
    18001 attaaatatg acgccaatta ctcaagaaga atggcaaaga tacgatacgt attttgataa
    18061 aatgattgaa agaaacaaga aaatgataga tcaaatgcaa tagttaaata tttaacagcc
    18121 cctttcaagt tatcgtttgg ggctgttatt tttgaaagta tccataaaaa gcataaatat
    18181 gttattgacc taatatttaa attgaacatc attaaattaa aagtaatgat atgatggaaa
    18241 taataaattt aataagaaaa tttcatgttt tacgtattaa ataga
//
LOCUS       DAMQYT010000029        18246 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMQYT010000029 DAMQYT010000000
VERSION     DAMQYT010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18246
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.029"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            111..2038
                     /gene="abc-f"
                     /locus_tag="PGA33_000818"
                     /pseudo
     CDS             111..2038
                     /gene="abc-f"
                     /locus_tag="PGA33_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
     gene            complement(2182..2310)
                     /locus_tag="PGA33_000819"
     CDS             complement(2182..2310)
                     /locus_tag="PGA33_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472649.1"
                     /translation="MIKTMSIENMRTYQQRVNNLWIKGVDNVIYCGYQVGKQKIND"
     gene            2399..4009
                     /locus_tag="PGA33_000820"
     CDS             2399..4009
                     /locus_tag="PGA33_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HDG6472650.1"
                     /translation="MSTNQTFLIFVIAIILLTSVIGIVGRYMSRQRLLKSMETLWQTI
                     SPLEAFIRPNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMK
                     LYACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNIL
                     FLCPFLPVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLK
                     QGYTISKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLF
                     HSIQKSYTTHMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELT
                     HPLIADAVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPG
                     IVFTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAAS
                     ESVLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKAN
                     TRNAIELLKITSFPAKIYERAKDNVPKI"
     gene            complement(4111..5136)
                     /gene="tsaD"
                     /locus_tag="PGA33_000821"
     CDS             complement(4111..5136)
                     /gene="tsaD"
                     /locus_tag="PGA33_000821"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDG6472651.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITATINEALGDADVSIEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(5129..5593)
                     /gene="rimI"
                     /locus_tag="PGA33_000822"
     CDS             complement(5129..5593)
                     /gene="rimI"
                     /locus_tag="PGA33_000822"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDG6472652.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDLERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            complement(5566..6228)
                     /gene="tsaB"
                     /locus_tag="PGA33_000823"
     CDS             complement(5566..6228)
                     /gene="tsaB"
                     /locus_tag="PGA33_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDG6472653.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTVKLQDELQGEVIAQLPNASVMYQLLDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            complement(6209..6670)
                     /gene="tsaE"
                     /locus_tag="PGA33_000824"
     CDS             complement(6209..6670)
                     /gene="tsaE"
                     /locus_tag="PGA33_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDG6472654.1"
                     /translation="MIKINNLDEMNQFAIFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            6708..6863
                     /locus_tag="PGA33_000825"
     CDS             6708..6863
                     /locus_tag="PGA33_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472655.1"
                     /translation="MIIHCSIVSNFIFNLYIVTNFNLNFLSLKQIIYSKLIVRKFKIF
                     VDILKQI"
     gene            7181..8869
                     /gene="ilvD"
                     /locus_tag="PGA33_000826"
     CDS             7181..8869
                     /gene="ilvD"
                     /locus_tag="PGA33_000826"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDG6472656.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLTLPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            8897..10666
                     /gene="ilvB"
                     /locus_tag="PGA33_000827"
     CDS             8897..10666
                     /gene="ilvB"
                     /locus_tag="PGA33_000827"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDG6472657.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            10666..10920
                     /locus_tag="PGA33_000828"
     CDS             10666..10920
                     /locus_tag="PGA33_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDG6472658.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            11057..12061
                     /gene="ilvC"
                     /locus_tag="PGA33_000829"
     CDS             11057..12061
                     /gene="ilvC"
                     /locus_tag="PGA33_000829"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDG6472659.1"
                     /translation="MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVV
                     IGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNA
                     LAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQGASGQA
                     RNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGY
                     QPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVKENMKA
                     VLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK
                     "
     gene            12091..13620
                     /locus_tag="PGA33_000830"
     CDS             12091..13620
                     /locus_tag="PGA33_000830"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDG6472660.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            13623..14669
                     /gene="leuB"
                     /locus_tag="PGA33_000831"
     CDS             13623..14669
                     /gene="leuB"
                     /locus_tag="PGA33_000831"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDG6472661.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFVN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            14683..16053
                     /gene="leuC"
                     /locus_tag="PGA33_000832"
     CDS             14683..16053
                     /gene="leuC"
                     /locus_tag="PGA33_000832"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDG6472662.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            16054..16626
                     /gene="leuD"
                     /locus_tag="PGA33_000833"
     CDS             16054..16626
                     /gene="leuD"
                     /locus_tag="PGA33_000833"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDG6472663.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYVEIEVDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            16641..17909
                     /gene="ilvA"
                     /locus_tag="PGA33_000834"
     CDS             16641..17909
                     /gene="ilvA"
                     /locus_tag="PGA33_000834"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HDG6472664.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
     gene            complement(18119..18233)
                     /gene="rrf"
                     /locus_tag="PGA33_000835"
     rRNA            complement(18119..18233)
                     /gene="rrf"
                     /locus_tag="PGA33_000835"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6249 a         3064 c         3217 g         5716 t
ORIGIN      
        1 tatcttagtt tcaagacggc gttttatact gtaaaatggt tattaatgta ataattacga
       61 cttttttcat aagttaataa aatttgagaa agacgaaagg tgaagatcga atgatacttt
      121 tacaactcaa tcatatatca aaatcgttcg atggtgaaga tatatttact gatgttgatt
      181 ttgaagtaaa aactggtgaa cgaattggta tcgtaggaag aaatggcgcc ggtaaatcaa
      241 cattgatgaa aattatagct ggcgtagaaa actatgattc tggaaatgtg tcaaaaatta
      301 aaaacttaaa actaggttat ttaactcaac aaatgacttt aaattctaat gcaacagttt
      361 ttgaagaaat gtcaaaacca tttgaacata ttaaacgaat ggaaagctta atcaaagaag
      421 aaacagattg tttatcaaaa catgcaaatg attacgatag tgatacatat aaaacacata
      481 tgtcccgtta tgaatcttta tcgaatcaat ttgaacaatt agaaggatat caatatgaaa
      541 gtaaaattaa aacagtactt tacgggttaa attttagtga agaagatttc aataaaccta
      601 tcaatgattt tagcggtggc caaaaacacg tttatcttta gctcaaatgc tattaaacga
      661 acctgattta ttacttttag atgaacctac taaccactta gatttagaaa cgacaaagtg
      721 gcttgaagat tatctacgtt attttaaagg tgcaatcgtc atcatcagcc atgatcgtta
      781 ctttttagat aaaatagtta ctcaaattta tgatgtggct ttaggtgatg tcaaacgcta
      841 tgttggtaat tacgaggaat ttatacagca acgggattta tattatcaaa aacgaatgca
      901 agaatatgaa agtcaacaag cagaaataaa acgattagaa acttttgttg agaaaaatat
      961 tacccgtgct tcaacaagtg gaatggcaaa aagtagacgt aagattttag aaaaaatgga
     1021 acgcattgat aaaccaatgt tagatgccaa aagtgcaaat attcaatttg gctttgaccg
     1081 gaatacaggt aatgacgtca tgcatgtaaa aaatttagaa atcggttatc aaactgcaat
     1141 taccaaacct atgagtatag aggtctctaa aggcgatcat atagcaatca ttgggccaaa
     1201 tggtattgga aaatcgacct taattaaaac tattgctaat caacaaaaag cgcttaatgg
     1261 cgatattact ttcggcgcaa atttacaaat tggttattat gatcaaaagc aagcagaatt
     1321 taaatctagt aaaacgattt tagattatgt gtgggatcaa tatccgttaa tgaatgaaaa
     1381 agatattcga gcagttcttg gacgtttctt atttgtacaa gacgatgtta aaaagataat
     1441 taatgattta tctggtggtg aaaaagcacg tttactacta gcactactta tgttgcaacg
     1501 tgacaatgta cttattttag atgaacctac caaccacctc gatatagatt caaaagaaat
     1561 gttagagcaa gcactccaac attttgaagg aacaatacta tttgtttccc atgatcgtta
     1621 ctttattaac caattagcaa acaaggtatt tgatttaaca attgatggcg gaaagatgta
     1681 tttaggagat tatcaatatt acattgaaaa agttgaggaa gcggaagcgt tgaaagcgca
     1741 ccaagaagaa cagtcagtta acgtacaagc acataaaaag tctatggaac aatcgtcgta
     1801 tcataaccaa aaagaacaaa gacgtgaaca acgcaaactt gaacgacaaa tatccgagtg
     1861 tgaaaatgaa atagaaactt tagagactac tatcttacaa atagacgagc aattaaccca
     1921 accagaagtg tataacaatc cacaaaaagc aaacgaatta gctatccaaa aacaagatag
     1981 cgaacaaaaa ttagaacacg ctatgtctaa atgggaagaa ttacaacaaa aactataaaa
     2041 atatacataa ctctaactgt aaaatcgcta tagttagagt tattttttta caaattttaa
     2101 tttttttatt cacaataaaa acgctgatat tatagctatc cacagactta tcctagttac
     2161 ccacaagtaa attaacacta atcaatcatt tattttctgt tttcccacct gatatccaca
     2221 ataaatgacg ttatcaacac cttttatcca caagttattc actctttgtt gataagtacg
     2281 catgttctct attgacattg tttttatcaa aattcattgt taagacattt tctttatgaa
     2341 ataataataa tattgaagta tatttttatt attattaaaa ataaataagg ggatacttat
     2401 gagcacaaat caaacatttt taatatttgt tatagcaatt attctactta catctgtaat
     2461 aggaattgtt ggacgataca tgagtcgtca acgtctatta aaatctatgg aaacattatg
     2521 gcaaacgatt tctccattag aagcttttat cagaccgaac tcacatttcg actatgagta
     2581 taagctctac aaggaaaaat ttgaatcaca ttcattagtt gatgataaaa cttggtccga
     2641 cttaaatatg aatgcaatct ttcataagat gaattataat ttaacagcta ttggtgaaat
     2701 gaagctatat gcctgtttac gtggaatgct ttcaattacg aacaaatcat tacttagttt
     2761 atttaatgat aatgctgaat ttagaaaaaa cgtaacatat catttagctt tgattggtaa
     2821 aactgtttat ccaacatttc cagaccaaat cacaccggta aaacgtcaaa atatattgtt
     2881 tctatgcccg tttttaccag ttatctcatt tgcagttata tttattaatt cacaagttgg
     2941 tattttatta tttcttatga gttgcctatt taatatcatt ttatcagcaa ctttaaagcg
     3001 tacatatgag gatgacttaa aatctatctt ttatgcatcc aatgttttaa aacaaggcta
     3061 tactatctca aaaattaaac atgctccaca acctgaagtc aattttaagc aatttagaac
     3121 agcacgtcat ttaacgagtg ttttagctga agttaatgat gaagatatcg gagctatggt
     3181 aattaaacta gttaaactta ttttcatgct tgattatgtt ttatttcatt caattcaaaa
     3241 aagctataca acacatatga atgagcttaa aaattgtttc gattacatag ctgaattaga
     3301 taatcattat gcattagcaa tgtatcgtag aacgctagaa tgttatactg aaccacaaat
     3361 tgatgattca aatgatggca tagtattctc tgagttaaca catccactca tagcagatgc
     3421 agtagccaat gatttctcac tatcacaaaa tatactgtta actgggtcta atgcttcggg
     3481 taaatcaaca tttatgaaat ccatagcaat aaacattatt cttgcatcgg ctatacaaac
     3541 agttaccgca agcaaatttg tatatcaacc tggaattgtg ttcacgtcaa tggcaaatgc
     3601 cgatgatgtg ttatcaggcg atagttattt tatggctgaa ttaaaatcta ttaagcgtat
     3661 tgttgaaatt ccagataatc aaaaaatata ctgctttata gatgaaattt ttaaaggtac
     3721 caacacaact gaacgaattg ccgcttcaga atcagtacta tcatttttac atgaaaaatc
     3781 taactttaga gttattgcag caacacatga tattgagtta gctgaactct taaaacaacg
     3841 ttatgaaaat taccatttca atgaggtaat agaaaataat aacatacatt ttgattacaa
     3901 aattaagcct ggcaaagcaa atacacgtaa tgccatcgaa ttattaaaaa tcacttcatt
     3961 tccagcaaaa atatatgaac gagcaaaaga taatgtcccg aaaatttagc atttaacttt
     4021 aaacataaaa acgtcagcta tcacatgaca gaagactatg aacagtttca ataatgttca
     4081 tagtaatcat gttaataact gacgtttatt ttattctgca gaatactctt ctaaatctat
     4141 attgctgtgc ccatttaatg ctaaatcagc aaatcgacct tgctgataca aatagtggcc
     4201 ggcaacgcct atcattgcag cattatctgt gcataattta ggacttggga tagttaattg
     4261 aatgtcattg actttgcatt gatccgctaa agattgacgt aatcctttat tactcgccac
     4321 gccaccagca acaattaatc gctgaacacc atattcttta caagcttgaa tagctttaaa
     4381 cgtgagcacc tctacaacac tgttttgaaa gctcgttgct acgttagctt caatgattgg
     4441 aatatttttt tgtcgttgat tgtgaagttg attgattacg gcacttttca acccactaaa
     4501 actaaaatca taactatctt tatccaacca aacacgaggg aatgaataag tatcttcacc
     4561 ttcagcagcc aaccgatcaa cttgtggacc acctggataa tttaaaccaa ttgttcgtgc
     4621 cactttatca taagcctcac ctactgcgtc atctcgtgtt tcaccaatga cttcaaatga
     4681 taaatgatcc ttcatataaa ctaattcagt atgtccacct gaaacaataa gtgcaattag
     4741 cgggaatgtt aatggctctt ctatgtgatt agcatatata tgtcctgcaa tatgatgaac
     4801 aggaataagt ggcttatcgt aagcaaatgc caatgctttg gctgcattaa cacctattag
     4861 taacgcacca attagtccag ggccttctgt aaccgctatg gcatcaatat cttctattga
     4921 tacatcggca tcccctagag cctcgtttat tgttgctgtt ataccttcaa cgtgatgtct
     4981 acttgccact tcgggaacga caccgccaaa tcgtttatga ctttcaatct gacttaaaac
     5041 tgtatttgat aaaatatctc tgccattttt tataacacta acgcttgttt catcacaact
     5101 tgtttcaaca gctagtatta atatatcttt agtcatttaa attcacccac ataaccattg
     5161 cgtcctcacc ttcaccataa taatttttac gtttaccacc atattgaaat cctaaatttt
     5221 catatacatg ttgtgccact ttattattaa ctcttacttc taaactcatc acatcacaag
     5281 tgtgacttgc atagtttatt ccgtatttta aaagcatttg acctaaacca tagcctctat
     5341 aattatcatc gattgcaact gttgtaattt gagcttgatc gataacaatc cataaaccta
     5401 aataaccaat aatttgttgt tcaaattcta agacaaaata tttcgcaaag ttattttgct
     5461 ctatttcatg ataaaatgcg tcaattgtcc aagaactgtc attgaaactc cgacgctcaa
     5521 gatcaaagac ttgtggcaca tcttctttag tcatctctct aatgtttaat tgttcttttg
     5581 actgttgatc caatttcgtt ccgcctcagc taatttatgg tatttaggag taaatgtatg
     5641 tacgtctgaa ggtttatcta gcaattgata catgactgat gcatttggta gctgcgcaat
     5701 cacttcacct tgtaattcat cttgtaattt tacagtatct ttcccaatat aaataaatgg
     5761 ttggtttaaa tcttctaaaa aagctcgcaa tgcctctatc gacatatatt gatcttctaa
     5821 aatagtcact aattgaccat tttgccactg gaatatgcct gtataaactg cttgtcgtct
     5881 tgcatcaaac acaggaacca ataatttatc agtatgatcg attgttgctg ccaatgcctt
     5941 taatgatgaa acaccatata atttaacatc taacgcatac gctaatgttt tagcaacagt
     6001 aacaccgata cgtaagccag tatatgaacc aggaccttca gcaacaataa tcgcatctaa
     6061 ttgctgttta gctattttgc tctgctcaaa tagctgacta atcgcaggca ttaattgcac
     6121 tgaatgattt tgtttagaat ccgtagttat ttcagctaaa acttcatcgt tttgcatcaa
     6181 tgctactgat aatggttgat tcgatgtatc aatgagcagc gaattcatgg ataattgcct
     6241 ccttaatttg ttcataatgt tctccttgcg cgaacaactc aatttgtctt gtattttcag
     6301 atattgttga aatgttaata gataaatgcg tcgctggaag taaatctttt ataaattgac
     6361 tccattcaat aacagtaatt gcctgatctt cgaaaaattc atcaaatcct aaatcttcat
     6421 cagaatcttc taagcgataa caatccatat gatgcaattt taaattttta cccctatatg
     6481 atttaatgat gttaaatgtc ggggaattaa tcgtacgtct tacaccaaga gcttttccta
     6541 taaattgcgt taacgttgtt ttacctgctc ctaaatctcc gttaagtaaa atcaaatcac
     6601 cacttttcaa ttgctcaact aaaaatatag caaattgatt catttcatct aaattattta
     6661 tctttatcaa tgttgattct cctatattat gcttttcatt cataaaaatg attatccatt
     6721 gttcaatcgt atctaacttt atatttaacc tttatattgt aacaaatttc aacttaaatt
     6781 tcttatcttt gaaacagatt atctattcaa agttaattgt aagaaaattt aaaatatttg
     6841 ttgacatact aaagcagata tagtaaatta aatttatcaa atttttagac aattctaact
     6901 attaaagtga tatataccat tcacggaagg agtataataa aatgcttaat caatatactg
     6961 aacatcaacc gacaacttca aatattatta ttttattata ctctttagga ctcgaacgtt
     7021 agtaaatatt tactaaacgc tttaagtcct atttctgttt gaatgggact tgtaaacgtc
     7081 ccaataatat tgggacgttt ttttatgttt tatctttcaa ttacttattt ttattactat
     7141 aaaacatgat taatcattaa aatttacggg ggaatttact atgcgaagcg acatgatcaa
     7201 aaaaggagat caccaagcac cagcaagaag tcttttacat gccacgggcg cgctaaaaag
     7261 tccaactgat atgaacaaac catttgtagc tatttgtaac tcttatattg atattgttcc
     7321 tggacatgtt cacttgagag agcttgcaga tatagctaaa gaagcaatta gagaagccgg
     7381 tgccattcca tttgaattca atacaattgg tgttgatgat ggaatagcta tgggacatat
     7441 cggaatgcga tattctctac catcacgtga aattattgca gatgcagctg aaactgtaat
     7501 taacgctcat tggtttgacg gcgtatttta cattcctaat tgtgacaaga ttacacccgg
     7561 tatgatttta gcagccatga ggacaaacgt accagctatc ttttgctctg gtggaccaat
     7621 gaaagctggc ttatctgcac atggaaaagc attaacactt tcatcaatgt ttgaagcagt
     7681 cggcgcattt aaagaaggat cgatttctaa agaagaattt ttagatatgg aacaaaatgc
     7741 ctgccctact tgtggttcat gtgctgggat gtttactgca aattcaatga actgtttgat
     7801 ggaagtttta ggtctaacat taccatacaa cggtactgca cttgcagtca gtgatcagcg
     7861 acgagaaatg attcgccaag cagcttttaa attagttgaa aatattaaaa atgatttaaa
     7921 accacgtgat atcgttactc gcgaagcaat tgatgatgca tttgcacttg atatggctat
     7981 gggtggttca acaaacacgg tactgcatac gttagccatt gccaatgaag ctggtattga
     8041 ttatgactta gagcgcatta atgctattgc caaacgcacg ccatatttat caaaaatagc
     8101 acctagttca tcgtattcaa tgcatgatgt gcatgaagct ggtggcgtcc cagcaattat
     8161 taatgaattg atgaagaaag atggcacgtt acacccagat agaatcacag ttactggcaa
     8221 aacgttacgt gaaaataacg aaggcaaaga aattaagaac tttgatgtca ttcaccctct
     8281 tgatgcacca tatgatgcac aaggcggttt atctatctta tttggtaata tcgcccctaa
     8341 aggcgcagtt attaaagttg gcggcgttga tccatctatc aaaacattta ctgggaaagc
     8401 aatttgtttc aattcgcatg atgaagctgt tgaagcaata gacaatcgta ccgttcgtgc
     8461 aggccacgtc gttgtcatta gatatgaagg acctaaaggt ggaccaggta tgcctgaaat
     8521 gttagcacct acttcctcta ttgttggtcg cggcttaggt aaagatgttg cattaattac
     8581 tgatgggcgt ttttccggtg ccacaagagg tattgcagtt ggtcatattt cccctgaagc
     8641 tgcatctggt ggaccaattg ccttaattga agatggtgat gagattacta ttgatttaac
     8701 aaatcgtaca ttaaacgtaa accagcctga agatgttcta gcgcgtcgcc gagaatcttt
     8761 aacaccattt aaagcgaaag taaaaacagg ttatctagct cgttatactg ccctagtaac
     8821 tagcgcaaat acaggtggcg tcatgcaagt ccctgagaat ttaatttaat ttatttttat
     8881 attggagatg gttaaaatgt ctaaaactca acatgaagta aaccaaaata ttgacccttt
     8941 aaaaatggct gaatcacttg aacctgaaca actaaatgaa aaaactttaa atgatatgcg
     9001 ttcaggatca gaagtgctag tagaagctct acttaaagaa aatgtggatt atttattcgg
     9061 ttatcctggt ggtgccgtac tacctttata tgacacgttt tatgatggta aaatcaaaca
     9121 tattttagca agacacgaac aaggtgctgt tcatgctgca gaaggttatg cacgtgtatc
     9181 tggtaaaact ggcgtcgttg tagttacaag cggtccaggt gcaactaatg taatgacagg
     9241 tattacggat gcacattgcg actctttacc tctagttgta ttcactggac aagttgctac
     9301 accaggcatt ggtaaagatg cattccaaga agcggatatt ctatctatga cttcaccaat
     9361 tacaaaacaa aattatcaag tgaaacgtgt tgaagatatc cctaaaatcg tacacgaagc
     9421 tttccatgta gctaattctg gacgcaaagg tcctgtagtg attgattttc caaaagatat
     9481 gggtgtttta gctacaaatg tggatttatg cgacgaaatc aatattccag gttatgaagt
     9541 tgttacagaa ccagaaaata aagacattga cactttcatc tcacttttaa aagaagcgaa
     9601 aaagcctgtc gtattagccg gcgcaggtat taatcaatca aaatcaaatc aattattaac
     9661 acagtttgtt aataaacatc aaatcccaac agttactact ttactaggtc taggtgctgt
     9721 accttatgag gatacactat ttttaggtat gggaggaatg catggttctt atgctagtaa
     9781 catggcatta actgagtgtg atttactcat taatttaggt agccgcttcg atgatagatt
     9841 agcaagcaaa cctgatgcct ttgcacctaa cgccaaaatt gtacatgtag atattgatcc
     9901 ttcagaaatc aataaagtta ttcatgtaga tttaggtatt attgcagact gtaaaagatt
     9961 tttagaatgt ttaaatgata aaaatgttga gactatagaa cacagtgact gggttaaaca
    10021 ttgtcaaaat aataagcaga aacacccatt taaacttggt gaagaagatc aagtattttg
    10081 taagccacaa caaacaatcg aatatatcgg caaaattaca aatggtgaag caattgttac
    10141 tacagacgtg ggacaacatc aaatgtgggc agctcaattt tatccattta aaaatcacgg
    10201 acaatgggtt acaagcggtg gtttaggaac aatgggattc ggtattcctt cgtcaattgg
    10261 tgccaaatta gctaatcctg ataaaacagt cgtatgtttc gtcggtgacg gtggtttcca
    10321 aatgacaaac caagaaatgg cacttttacc cgaatatggt ttagatgtca aaatcgtact
    10381 aatcaataat ggaacattag gtatggttaa acaatggcaa gataagttct ttaatcaacg
    10441 cttctcacac tcagtattta atggtcaacc tgattttatg aaaatggcag aagcatatgg
    10501 cgtcaaaggt ttcttaatcg ataagccaga acaactggaa gaacaattag atgcagcgtt
    10561 tgcttatcaa ggaccagctt taattgaggt tcgtatttcc cctactgaag ctgtaacccc
    10621 aatggttccg agtggcaaat caaatcatga aatggagggc ttataatgac aagaattctt
    10681 aaattacaag ttgcggatca agtcagcacg ctaaatcgaa ttacaagtgc ttttgttcgc
    10741 ctacaatata atatcgatac attacatgtt acacattctg aacaacctgg gatttctaac
    10801 atggaaattc aagtcgatat tcaagatgat acatcacttc atatattaat taaaaaatta
    10861 aaacaacaaa ttaatgtttt aacggttgaa tgctacgacc ttgttgataa cgaagcttaa
    10921 ttttaagaca aaggcaatga tgcgctaatt agttatagat atatcatagg ctgctagtta
    10981 acatctgcca ctattacaaa gttatatttc agaattttcg aaacacaaaa tatttaatta
    11041 tttggaggaa tttattatga caacagttta ttatgatcaa gatgtaaaaa cggacgcttt
    11101 acaaggcaaa aaaattgcag tagtaggtta tggatcacaa ggtcacgcgc atgcacaaaa
    11161 cttaaaagac aatggatatg atgtagtcat cggcattcgc ccaggtcgtt cttttgacaa
    11221 agctaaagaa gatggatttg atgtgttccc tgttgcagaa gcagttaagc aagctgatgt
    11281 aattatggtg ctattacctg atgaaattca aggtgatgta tacaaaaacg aaattgaacc
    11341 aaatttagaa aaacataatg cgcttgcatt tgctcatggc tttaacattc attttggtgt
    11401 tattcaacca ccagctgatg ttgatgtatt tttagtagct cctaaaggac cgggtcattt
    11461 agttagacgt acatttgttg aaggttctgc tgtaccatca ctatttggta ttcaacaagg
    11521 cgcttcaggt caagcacgta atattgcttt aagttatgca aaaggtattg gtgcaactcg
    11581 tgcaggtgtt attgaaacaa catttaaaga agaaactgag acagatttat ttggtgaaca
    11641 agcagtactt tgcggtggtg tatcgaaatt aattcaaagt ggctttgaaa cattagtaga
    11701 agcgggttat caaccagaat tagcttattt tgaagtatta catgaaatga aattaatcgt
    11761 tgatttgatg tatgaaggcg gtatggaaaa tgtacgttac tcaatttcaa atactgctga
    11821 atttggtgac tatgtttcag gaccacgtgt tatcacacca gatgttaaag aaaatatgaa
    11881 agctgtatta actgatatcc aaaatggtaa cttcagtaat cgctttatcg aagacaataa
    11941 aaatggattc aaagaatttt ataaattacg cgaagaacaa catggtcatc aaattgaaaa
    12001 agttggtcgt gaattacgcg aaatgatgcc ttttattaaa tctaaaagca ttgaaaaata
    12061 agatagacct acaatgagga gttgttaaat atgagtagtc atattcaaat ttttgatacg
    12121 acactaagag acggtgaaca aacaccagga gtgaatttta cttttgatga acgcttgcgt
    12181 attgcattgc aattagaaaa atggggtgta gatgttattg aagctggatt tcctgcttca
    12241 agtacaggta gctttaaatc tgttcaagca attgcacaaa cattaacaac aacggctgta
    12301 tgtggtttag ctagatgtaa aaaatctgac atcgatgctg tatatgaagc aacaaaagat
    12361 gcagcgaagc cggtcgtgca tgtttttata gcaacatcac ctattcatct tgaacataaa
    12421 cttaaaatgt ctcaagaaga cgttttagca tctattaaag aacatgtcac atacgcgaaa
    12481 caattatttg acgttgttca attttcacct gaagatgcaa cgcgtactga attaccattc
    12541 ttagtgaaat gtgtacaaac tgccgttgac gctggagcta cagttattaa tattcctgat
    12601 acagtcggct acagttacca tgatgaatat gcacatattt tcaaaacctt aacagaatct
    12661 gtaacatctt caaatgaaat tatttatagt gctcattgcc atgacgattt aggaatggct
    12721 gtttcaaata gtttagctgc aattgaaggc ggtgcgagac gaattgaagg cactgtaaat
    12781 ggtattggtg aacgagcagg taatgcagca cttgaagaag tcgcgcttgc actatacgtt
    12841 cgaaatgatc attatggtgc tcaaactgct cttaatctcg aagaaactaa aaaaacatcg
    12901 gatttaattt caagatatgc aggtattcga gtgcctagaa ataaagcaat tgttggccaa
    12961 aatgcattta gtcatgaatc aggtattcac caagatggcg tattaaaaca tcgtgaaaca
    13021 tatgaaatta tgacacctca acttgttggt gtaagcacga ctgaacttcc attaggaaaa
    13081 ttatctggta aacacgcctt ctcagagaag ttaaaagcat taggttatga cattgataaa
    13141 gaagcgcaaa tagatttatt taaacaattc aaggccattg cggacaaaaa gaaatctgtt
    13201 tcagatagag atattcatgc gattattcaa ggttctgagc atgagcatca agcactttat
    13261 aaattggaaa cactgcaact acaatatgtc tctagcggcc ttcaaagtgc tgttgttgtt
    13321 gttaaagata aagagggtca tatttaccag gattcaagta ttggtactgg ttcaatcgta
    13381 gcaatttaca atgcagttga tcgtattttc cagaaagaaa cagaattaat tgattatcgt
    13441 attaattctg tcactgaagg tactgatgcc caagcagaag tacatgtaaa tttattgatt
    13501 gaaggtaaga ctgtcaatgg ctttggtatt gatcatgata ttttacaagc ctcttgtaaa
    13561 gcatacgtag aagcacatgc taaatttgca gctgaaaatg ttgagaaggt aggtaattaa
    13621 ttatgactta taacattgtt gccctacctg gtgatggaat cggtccagaa attttgaacg
    13681 gatctctatc attgcttgaa attataagta ataaatataa ctttaattat caaatagagc
    13741 accacgaatt tggtggtgcc tctattgata cattcggcga gcctttaact gagaaaacct
    13801 taaatgcgtg taaaagagca gatgctattt tactgggtgc aatcggtgga cctaaatgga
    13861 cagatcctaa caatcgacca gaacaaggat tattaaaatt gcgtaaatcc ttaaatttat
    13921 ttgtaaatat acgccccact accgttgtca aaggcgctag ttctttatca cctttaaagg
    13981 aagaacgcgt tgaaggcaca gatttagtta tagtccgtga attgacaagt ggtatttatt
    14041 ttggagaacc tagacatttt aataatcacg aggccttaga ttctcttact tatacaagag
    14101 aagaaataga acgcattgtt cacgtagcat ttaaattggc cgcttcaaga cgaggaaaac
    14161 taacatcagt tgataaagaa aatgtattag cttctagtaa attgtggcgc aaagtcgtaa
    14221 atgaagtaag tcaattatat ccagaagtaa cagtaaatca cttacttgtt gatgcttgta
    14281 gtatgcattt aatcacaaat ccaaaacaat ttgacgtcat cgtatgtgaa aacttatttg
    14341 gcgatatttt aagtgatgaa gcttcagtga ttcctggttc acttggttta tcaccttctg
    14401 ctagttttag taacgatggt ccaagattgt atgagcctat tcatggatca gcaccagata
    14461 ttgcaggtaa aaacgttgcc aatccatttg gaatgattct atctttagcg atgtgtttac
    14521 gtgaaagctt aaatcaacca gatgctgcag atgaattaga acaacatatt tatagcatga
    14581 ttgaacatgg gcaaacgaca gcagatttag gcggcaaatt gaatactact gatattttcg
    14641 aaattctatc tcaaaaattg aatcactaag ggggagatgt aaatgggtca aacattattt
    14701 gacaaggtgt ggaacagaca tgtgttatac gggaaattgg gcgaaccgca actattatac
    14761 attgatttac accttataca tgaagttact tctcctcaag catttgaagg acttaggctt
    14821 caaaacagaa aattaagacg cccagattta acatttgcaa cactcgatca caatgttcct
    14881 actattgata tattcaatat taaagatgaa attgcaaaca aacaaatcac aacattacaa
    14941 aaaaacgcca tagattttgg ggtgcatatt tttgatatgg gttctgatga acaaggtatt
    15001 gttcacatgg taggacctga gacaggactt acacagcctg gcaagacaat cgtttgtggt
    15061 gactctcaca cagcaacaca tggagcattt ggtgctattg catttggaat tggaacaagt
    15121 gaagttgaac atgttttcgc aactcaaacg ctatggcaaa caaaacccaa aaacttaaaa
    15181 atcgatatta atggtacctt accaacaggc gtctatgcta aggacattat tctgcattta
    15241 attaaaacgt atggtgttga ctttggtaca ggctatgctt tggaatttac tggcgaaaca
    15301 attaaaaacc tttcaatgga tggtcgaatg actatttgta acatggctat cgaaggtggt
    15361 gccaaatacg gcataatcca acctgatgat ataacatttg aatatgttaa agggagacca
    15421 tttgccgata acttcgctaa atcagttgat aagtggcgtg agctatattc tgatgacgac
    15481 gcgatatttg atcgtgtaat tgaacttgat gtttcaacat tagaaccaca agtgacatgg
    15541 ggaactaatc ctgaaatggg tgttaatttc agtgaaccat tccctgaaat caatgatatc
    15601 aacgatcaac gtgcgtatga ttatatgggg ttagaaccag gtcaaaaagc tgaagacatc
    15661 gacttagggt atgtttttct cggttcatgt acaaatgcta gactatcaga tttgattgaa
    15721 gctagtcata ttgttaaagg aaataaagtt catccaaata ttacagctat tgtcgtacca
    15781 ggttctcgta cagtaaaaaa agaagcagaa aaattaggtc tagatactat ctttaaaaat
    15841 gcaggatttg aatggcgtga accaggatgt tcaatgtgtt taggcatgaa tcctgaccaa
    15901 gtacctgagg gcgtacattg tgcatctaca agtaatcgaa actttgaagg acgacaaggc
    15961 aaaggtgcaa gaacacattt agtatcccct gctatggcag cagcagcagc tattcatggt
    16021 aaatttgtgg acgtaagaaa ggtggttgtt taaatggcag caatcaaacc tattacaaca
    16081 tataaaggta aaatagtccc tctcttcaac gacaatatcg atacagacca aatcattcct
    16141 aaggtacact taaagcgtat ttcaaaaagt ggctttggtc catttgcttt tgatgaatgg
    16201 cggtacttac ctgatggttc agataatcct gatttcaatc ctaacaaacc acaatataaa
    16261 ggggcttcta ttttaattac tggagataat tttggatgtg gttcaagtcg tgaacatgct
    16321 gcttgggctc ttaaggacta tggttttcat attattattg caggaagttt cagtgacata
    16381 ttttatatga attgcactaa aaatgcgatg ttgcctatcg ttttagaaaa aagtgcccgt
    16441 gaacatcttg cacaatatgt tgaaattgag gtcgatttac caaatcaaac tgtgtcatca
    16501 ccagacaagc gtttccattt tgaaattgat gaaacttgga agaataaact tgtaaatggc
    16561 ttagatgaca ttgcaatcac cctacaatat gaatcattaa tagaaaaata tgaaaaatca
    16621 ctttaaggga gttgaatatt atgacagtca aaacaacagt ttctacgaaa gatatcgatg
    16681 aggcattttt aagacttaaa gatattgtca aagaaacacc tttacaatta gaccattact
    16741 tatctcaaaa gtatgattgt aaagtctatt taaaacgaga agatttacaa tgggtacgtt
    16801 cttttaaatt aagaggtgct tacaacgcta tttctgtttt atcagatgaa gctaaaagta
    16861 aaggtattac atgtgcaagt gcaggtaatc atgctcaagg tgttgcctat acagctaaaa
    16921 aacttaattt aaacgctgtt atctttatgc cagtcactac acctttacaa aaggtaaatc
    16981 aagtaaagtt ctttggaaat agtaacgttg aagttgtact cactggtgat acatttgatc
    17041 actgtttagc tgaagcttta acttatacaa gtgaacatca aatgaacttt atagatccat
    17101 tcaataatgt tcatacaatt tctggacaag gtacgcttgc taaagaaatg ctagaacaag
    17161 caaagtctga caatgttaac tttgattatc tatttgccgc aattggtggt ggcggtttaa
    17221 tttcaggtat tagtacttac tttaaaacct attcacctac cacgaaaatt ataggtgttg
    17281 aaccttcagg tgcaagtagt atgtatgaat ctgttgtggt aaataatcag gtagtcacat
    17341 tgcctaatat cgataaattt gtggacggtg catctgtagc tagagttggc gatattacat
    17401 ttgaaattgc aaaagaaaat gtagatgatt acgttcaagt agatgaaggt gcagtttgtt
    17461 ctacgatttt agatatgtat tcaaaacaag caattgtagc agaacctgct ggcgcattaa
    17521 gtgtaagtgc gcttgaaaac tataaagatc atattaaagg taaaacagtg gtttgtgtca
    17581 ttagtggtgg taataatgat attaatcgaa tgaaagaaat tgaagaacgt tcattactat
    17641 acgaagaaat gaagcattac tttatcttaa atttccctca acgtccaggt gcattgagag
    17701 aatttgtaaa tgacgtatta ggacctcaag acgatattac taaatttgaa tacttaaaaa
    17761 aatcttctca aaatacaggt actgtcatta ttggtattca acttaaagat catgatgatt
    17821 taatacaact caaacaacgt gtaaatcatt tcgatccttc caatatttat attaatgaaa
    17881 ataagatgtt atattcattg ttaatttaac acatagtaag aaaaacagtc ataaattgat
    17941 ttctaattga aatcatctta tgactgcttt ttattatact ttacatttct cgtttcgtca
    18001 gattcaaacg ttttcacttc gccaagccat ctttctttgt gtttgctttt attttgacgt
    18061 tttagacata aaaaaagaga ccttgcggtc tcaatgcggc tcatcgcatc cactttttgc
    18121 ctggcaacgt tctactctag cggaacgtaa gttcgactac catcgacgct aaggagctta
    18181 acttctgtgt tcggcatggg aacaggtgtg acctccttgc tatagtcacc agacatatga
    18241 atgtaa
//
LOCUS       DAMQYT010000030        17299 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMQYT010000030 DAMQYT010000000
VERSION     DAMQYT010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.030"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            138..860
                     /locus_tag="PGA33_000836"
     CDS             138..860
                     /locus_tag="PGA33_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDG6472665.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            complement(1046..1321)
                     /locus_tag="PGA33_000837"
     CDS             complement(1046..1321)
                     /locus_tag="PGA33_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472666.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(1476..2540)
                     /locus_tag="PGA33_000838"
     CDS             complement(1476..2540)
                     /locus_tag="PGA33_000838"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDG6472667.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTAFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            2758..3624
                     /locus_tag="PGA33_000839"
     CDS             2758..3624
                     /locus_tag="PGA33_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDG6472668.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRLDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDKILQDTTS"
     gene            3966..4244
                     /locus_tag="PGA33_000840"
     CDS             3966..4244
                     /locus_tag="PGA33_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HDG6472669.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLMSKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
     gene            4259..4627
                     /locus_tag="PGA33_000841"
     CDS             4259..4627
                     /locus_tag="PGA33_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472670.1"
                     /translation="MSNKLDEINKIITAKHEQMDDLYDEKREVKALIDESDALNHSID
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKVEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            4615..4911
                     /locus_tag="PGA33_000842"
     CDS             4615..4911
                     /locus_tag="PGA33_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HDG6472671.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTQQ"
     gene            5245..6351
                     /locus_tag="PGA33_000843"
     CDS             5245..6351
                     /locus_tag="PGA33_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HDG6472672.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTEIYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVLTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQDVIENYQKNQMYLDDYSCCEVSCTC"
     gene            6345..7685
                     /locus_tag="PGA33_000844"
     CDS             6345..7685
                     /locus_tag="PGA33_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HDG6472673.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            7783..8673
                     /locus_tag="PGA33_000845"
     CDS             7783..8673
                     /locus_tag="PGA33_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDG6472674.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQ
                     SPVKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(8820..9650)
                     /locus_tag="PGA33_000846"
     CDS             complement(8820..9650)
                     /locus_tag="PGA33_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDG6472675.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWLEFEIRQYTHSNITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(9670..10680)
                     /locus_tag="PGA33_000847"
     CDS             complement(9670..10680)
                     /locus_tag="PGA33_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDG6472676.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSP
                     NN"
     gene            complement(10778..11482)
                     /locus_tag="PGA33_000848"
     CDS             complement(10778..11482)
                     /locus_tag="PGA33_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDG6472677.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            complement(11475..12032)
                     /locus_tag="PGA33_000849"
     CDS             complement(11475..12032)
                     /locus_tag="PGA33_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDG6472678.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVGRND"
     gene            12163..12462
                     /locus_tag="PGA33_000850"
     CDS             12163..12462
                     /locus_tag="PGA33_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HDG6472679.1"
                     /translation="MSLNKEQRRITAEELQAHFEASTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            12482..13303
                     /locus_tag="PGA33_000851"
     CDS             12482..13303
                     /locus_tag="PGA33_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HDG6472680.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK"
     gene            13320..13697
                     /locus_tag="PGA33_000852"
     CDS             13320..13697
                     /locus_tag="PGA33_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDG6472681.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(13847..14077)
                     /locus_tag="PGA33_000853"
     CDS             complement(13847..14077)
                     /locus_tag="PGA33_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDG6472682.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(14297..14797)
                     /locus_tag="PGA33_000854"
     CDS             complement(14297..14797)
                     /locus_tag="PGA33_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472683.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICACLLANVRHSSIVCLNNIC
                     CIVDMRCLNTKIGGIDVSETDEPQGFERTHNILNINQSSLGVVTYITNKLKSTLKQHI
                     IIARGKKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            complement(14995..15564)
                     /locus_tag="PGA33_000855"
     CDS             complement(14995..15564)
                     /locus_tag="PGA33_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDG6472684.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            15719..15847
                     /locus_tag="PGA33_000856"
     CDS             15719..15847
                     /locus_tag="PGA33_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472685.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSENSEFCDILFFIVLSYR"
     gene            15974..17029
                     /locus_tag="PGA33_000857"
     CDS             15974..17029
                     /locus_tag="PGA33_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDG6472686.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
BASE COUNT         5734 a         2649 c         3181 g         5735 t
ORIGIN      
        1 gtttaaaaaa tgcttttaga tacataaggg attttcgaaa tataaatgca ttaaaatgaa
       61 tttctaactt ttttgactga catttaatat gaatcatata taatagaagt taatgattaa
      121 atgtgggagg ttaaatcatg attaatgctg aacctattat cagtaaaatg aaaaatcaaa
      181 aaattaatta tgacaaggtg ttgaagaaat taatcgggca gtgggaaaga gaagcaatca
      241 gacctaaaat cttattgcat agttgttgtg caccttgtag tacatataca ttagagtttt
      301 taacacaata tgcagacatt gcaatttatt tcgcgaattc aaatattcat ccgaaaaatg
      361 agtacttacg acgtgctaaa gtgcaagaac aatttgtgga agattttaat cgcaagactg
      421 gtgcgaatgt gaagtatatt gaagcacctt atgaaccgca taagtttgtg aaaatggtga
      481 aggataaaga attagctgat gaaaaagaag gcggcttacg ttgtaccgct tgttttgaaa
      541 tgcgtttgga tattgtagcg aaagcagctg tagaacatgg ctatgattat tttggcagtg
      601 caatcacatt atcacctaaa aagaacgcac aattaatcaa tgaacttggt atggatgtcc
      661 aaaaaatata cgatgtgaac tatttgccaa gtgattttaa gaaaagtaaa ggttatgagc
      721 gttccataga aatgtgtaat gactataata tctttaggca gtgttattgt ggatgtgtct
      781 ttgcagcgat gcaacaaggt attgatttta aaacagttaa taaagaagcg aaagcatttt
      841 tagaacaata tccagattaa tcattggaaa tgagataaaa ggcaagaacg tatacatgcc
      901 atatcccatt aaaaattatc aaatagaaac ccgagatttt agtgaaatga atcatactaa
      961 aatctcgggt tatttatata atcgtaaata ttgtagatat aatgaataac tggatcctga
     1021 gacataaaac aatgtctcac tctaattaca tcattttatg aacaaatctt ataattgcta
     1081 aaatgacaat gacaagtgtt gaaataagtg aaaacacttt aaacttttta tagtcatttt
     1141 tcgtttcatt ttctcctgtt ttcatcattt cacgtagctc ttgctcgatt tctaaatcaa
     1201 gatctgtttt acgataacca ttcaaacctt gacgttcatc ttcttcaggt tgattttgtt
     1261 gctgtgtgtc tttgttgtca gaagtcgcta ctgttttttt attatctgtt tctttagtca
     1321 taacaaacgc ctccgttata aaacgctata tttaatgata tgtgatattt aagccattat
     1381 tttgaaaaat ggtgataaat atatcttctt gagttatatc ataattttaa taagacgatt
     1441 cagcaagttt taaagtatta tttgactatg ttggattagg catctagtcc tataatatca
     1501 ctgacattgt caaaatgatg atcttttaag taacgtgcga tgcctttgtt cattttctta
     1561 gttaaacctg ggccttcaat aacaagtgat gaataaattt gaataagtga cgcaccgtga
     1621 cgcatcattt tgattgcatc ttcagtactg aatacgccgc ctgtacctat aattaaaaat
     1681 tcaccatttg tttgctgata agcatactta atcaatttta aattacgttc aaataatgga
     1741 cgaccactca aaccgccttc ttcgacttta ttagcagaag ttaaaccatc tcgttgtcgc
     1801 gttgtgtttg ctaagatgat accgtcaaat gtctcagtaa tcgctggtaa tagtgctttt
     1861 aagccatcga aatccatatc agacgttagt tttaaataaa ttggcactgt tacatcatgt
     1921 tgttttttaa atgctgttaa agcttggcat aacattgaaa attcatcttt atcatggaag
     1981 ttttgaagat tttcagtatt tggagaactg atgttgactg tgaaaaatga aacgtcgtgt
     2041 ttaaacgtat caataacctt tatataatct tgataacgcg cttcataagg tgtcatttta
     2101 ttcacaccaa cattgatacc aacaggtact tgataagcat ttttacgcaa atgacttagt
     2161 gctttgttca taccaatatt attgaagccc attcgattta tcaaggcgtc atcttctaat
     2221 aatctaaaca tgcgtggttg agggttaccc ggttgaggtt taggtgtgat accacctaat
     2281 tctaaagcac cgaatccaag gtgttccaat gcttttggta cttcgcaaga tttgtcgaaa
     2341 ccagctgcta agccaattgg attgtcgtac gtattacctt gtatcgtttg tgataacgtt
     2401 ggattcttat aagtaaatag tttatcgacg actgggaata aaaccggaaa cttttgtaac
     2461 gtttttaatg catcgatagt tagtccgtgt gctttttcgg gttcgatttt gaataagaaa
     2521 ggtttaatta atttgtacat gagtatgctc ctatttcatt atatttgagg cttactatcc
     2581 tcaacttaat atatgtgaaa tatattcttt taatagacta gcatttccat acataatttc
     2641 ctagttaaaa ctaaaaagtt ttgaaaattg acgcaagttt gaataacgtt tttaagatta
     2701 aatcatccta attaggcaat attatagtat aaagtaagta gattggaagg tgtttgtatg
     2761 aatgaacaat ggttagagca tttaccttta aaagatatta aagagatttc accagtgagt
     2821 ggtggtgatg taaacgaagc atatcgagtc gaaacagata cggatacatt tttcttactt
     2881 gtccaacgtg gacgtaaaga atcattttat gctgcagaaa ttgcaggttt aaatgaattt
     2941 gaacgtgcag gtatcacggc acctagagta attgcaagtg gcgaggttaa cggtgatgcg
     3001 tatttagtga tgacgtattt agaagaaggg gcttcaggga gtcaacgcca attagggcaa
     3061 ctcgtagctc aattacacag tcagcaacaa gaagaaggca aatttggctt ctcattacct
     3121 tatgaaggtg gcgatatttc ttttgataat cattggcaag acgattggtg taccattttt
     3181 gtcgacaaac gcttggacca tttgaaagat gagttgctaa atagagggct atgggatgcc
     3241 aacgatatca aagtatatga caaagtgcga cgtcaaattg tggcggaatt agaaaagcat
     3301 caaagtaaac cgtctttatt acatggtgac ctatggggtg gtaattatat gttcttacaa
     3361 gatggtcgtc cggcgttatt tgatccagcg ccattatatg gtgacagaga attcgatatc
     3421 ggtattacaa cggtatttgg tggttttacg agcgaatttt atgatgcgta taataaacat
     3481 tatccactcg caaaaggtgc atcctataga cttgaatttt atcgtttata tttattgatg
     3541 gtccatttat tgaaatttgg tgagatgtac cgtgatagtg ttgcgcattc tatggataag
     3601 attttacaag atacaacaag ttagttaaga cgttagattg agataaatag ataatatgca
     3661 cagatatttt tacaatgaga agcgatacag ctgcctcaat aaaaatattt gtgcgttttt
     3721 attgttggaa aataaaattt taatcgctat tgttaatttc tgtaatgtaa aacaaggttg
     3781 agttacaata aaagtgattt tataactttt tgttcaataa aattctagga atgatacata
     3841 tttattgata caataatttt gaatataatc ataaaacaat atttaagtat aattgaatgt
     3901 ttgaatatca tatattgata cagtttctaa taattttaaa ataatttaaa tggagagagg
     3961 tgtaaatgat gagtacagtt caaagtgata tttttaagac caatagtgca tcatcatcta
     4021 ttaaaagcgc tgttgaaaca tgtaataatg tgtcgaaacc ggataaagat gaaagtacaa
     4081 cagtaagtgg aaataataat gctcatagtg tgatagatga tttgatgagt aagaatcaat
     4141 ctgttgctga agcaatacga actgcgagcg ataatataca aaaagttggt gaggcttttg
     4201 accaaactga cgtaatgatt ggtaatgaaa ttggtaaaaa ttaaaacgtg gtgaaatgat
     4261 gtcgaataaa ctggatgaaa tcaataaaat aatcacagcg aaacatgagc aaatggatga
     4321 cttatatgat gaaaagcgag aggttaaagc attgatagat gaaagtgatg cgcttaatca
     4381 ttcgatagat caattatatc aacatttagg tgagcgttat tatagtagca atatggctag
     4441 tcgtatggaa cagttccgcg atgaatttca ttttgcgaaa cgacgttcaa cggaagcgtt
     4501 atacgagcag caacagcaaa ttcaacatgg cattcgtaaa gtggaagaag agatgattga
     4561 cttggaaatg cgaaggaatg ttgaaattga gacggtgaca aaggaggaaa ataaatggaa
     4621 acaataggaa gcattattta tttaaaagaa ggttcgcaaa agttaatgat tattaataga
     4681 ggaccaattg tagaaattga aaatcaaaag tatatgtttg actattctgc atgtaaatat
     4741 ccgattggtg ttgtagaaga tgaaatttat tattttaacg aggaaaatat agattcagtt
     4801 atttttaaag gttattctga tcaagatgag gttagatttc aagagttgtt tgaaaatatg
     4861 aaacaaaatt tggatagtga aatacaacgt ggagaagtta cacaacaata aagaaatact
     4921 ttttctttat tggggtggga cgacgaaata aattttgtaa aaatatgatt tctgtctcac
     4981 cgccaaaatg ttatctccaa gtactgaggc tggatgatac catgaaatgt cattcataat
     5041 catttgaact aaacgtagca gccttaaatt ttaaaaaaag acacatacca acttccgaaa
     5101 tgtagatgaa ttctctacaa taacggaagt ttttctttta atattgaaat ttctcaagga
     5161 taggtctata ctttataaat cgtaattatt acgatttata atcaaaaaca ataacttgaa
     5221 atagatcatt gagggagtgt taatatgcaa catcataaag tggctattat cggtgccggt
     5281 gctgcaggta taggtatggc cattacctta aaagatttcg gtataacaga tgtcattatt
     5341 ttagaaaaag gaacagtagg acattcattt aaacattggc cgaaatcgac ccgtacgatc
     5401 acgccatcat ttacgtctaa tggatttggc atgcctgata tgaatgcaat ttccatggat
     5461 acttcaccag catttacatt taatgaagaa catatttccg gagaaacata tgctgaatat
     5521 ttacaagtgg ttgccaacca ttacgagctg aatatctttg aaaatacagt tgtcacaaat
     5581 atatctgtag atgatgcata ttatacgatt gcaacgacaa cagagatata tcacgcggat
     5641 tatatctttg tcgcaacagg tgattataat ttccctaaaa agccatttaa atatggtatt
     5701 cattatagtg aaattgaaga ctttgataac tttaataagg ggcaatatgt ggttatcgga
     5761 ggtaatgaaa gtggctttga tgctgcatat caacttgcaa aaaatggctc tgacatcgca
     5821 ctttatacta gcacaaccgg tttaaatgat ccggatgctg atcctagtgt tagattgtca
     5881 ccttatacac gtcagcgact aggtaatgtc attaagcaag gtgctcgcat cgaaatgaat
     5941 gtacattata cagttaaaga tattgatttt aacaatggac agtatcatat cagttttgat
     6001 agcggacaaa gtgtgcttac acctcatgaa ccaatactag caactggctt tgatgcaaca
     6061 aaaaatccaa tcgttcaaca attatttgtg acaacaaatc aagatattaa attaacaaca
     6121 catgatgaat cgacacgtta tccgaatatt tttatgattg gtgcaacagt tgaaaatgat
     6181 aatgccaaat tatgctatat ctataaattt agagcgcgat ttgcagtact tgcacatctt
     6241 ttaacacagc gggaaggctt accagctaaa caagatgtca ttgaaaatta tcaaaaaaat
     6301 caaatgtatt tagatgatta ttcatgttgt gaagtgtcat gcacatgtta gaagtgaaat
     6361 atgatatgag aactgggcat tatacgccca tacctaatga acctcattat ttggttatta
     6421 gtcatgcgga taaacttacc gcaacagaaa aagcgaaatt aagattatta atcataaaac
     6481 agaaattaga tatttcattg gcagaaagtg tagtttcttc gcctatagcg agtgaacatg
     6541 tgatagaaca attgacacta tttcaacatg agcgacgaca tttaagacct aaaataagtg
     6601 cgacattttt agcctggttg ttgatatttt taatgtttgc attgccaatc ggtatcgctt
     6661 atcaattttc agattggttt caaaatcagt atgtgtcagc atggatagaa tatttaactc
     6721 aaacaacatt gctcaatcac gatatattac agcatatatt atttggtgat tatggtgtgc
     6781 tatcacttgg aacatattcg ctcgtatggg cattgccggt tgtaatattg attagtttat
     6841 caactgctat aattgatcaa acaggactca aatcatggat gatatgggca attgaaccgt
     6901 caatgttatg gataggatta caaggtaatg atatcgtgcc actattagaa gggtttggat
     6961 gtaatgcagc agctatttca caagcagcac accaatgcca tacctgcacg aagacacagt
     7021 gtatgagttt aataagcttt ggtagttctt gtagttatca aataggtgcg acattatcta
     7081 tttttagtgt agctggaaag tcatggctat ttatgccgta cttaatatta gtacttttag
     7141 gtggcatctt acataatagg atatggttga aaaagaatga tcaacaactt agcgttccgc
     7201 taccttatga taggcaatta catatgccaa atatacgtca aatgttgcta caaatgtggc
     7261 aaaatataca aatgtttatc gttcaagcgc tacctatttt tatcacaatc tgtcttattg
     7321 ttagtatttt atcactaacg ccaattttga atgttttatc acaaatattt acacctatat
     7381 tatcgttatt aggcatctcg tcagaattgt caccagggat tttattttca atgattcgaa
     7441 aagacggcat gctcttgttt aatttgcatc agggcgcctt attacaagga atgacagcaa
     7501 cacagttact actacttgtg ttttttagtt caacatttac agcgtgctcg gtcacaatga
     7561 cgatgctttt gaaacattta ggtggtcagt cagcactaaa attaattgga aagcaaatgg
     7621 tgacatcatt gtctttagtt attggtgtag gcatcattgt taaaatagta atgctgatta
     7681 tttaaaaaaa atgaactata actgaatata gagtcatgtc agtcaatagg agatctatct
     7741 tggaatatgc tattcatatg aagtataaga ggagagtcgc agatgaaaat agttattata
     7801 ggtgggtttt taggtggcgg taaaacgact gtcttaaatc atttgctcgc tgaatcatta
     7861 aaggaatcgc tgaaaccagc agtcatcatg aatgaatttg ggaaaatgag tgttgatggt
     7921 gccttagtat ctgaagacat acctttaagt gaactgacag aggggtgtat ctgttgtgca
     7981 atgaaagcag atgtatcaga acagttacat caattatatt taaaagagca accagacatt
     8041 gtatttattg aatgtagtgg gattgcagaa ccggtctctg tcttagatgc ttgtttaacg
     8101 cctattttag ctccgtttac aacaattaca catatgattg gtgtaataga cgcaagcatg
     8161 tataaacaca ttaaatcatt ccctaaagac atccaaggct tattttatga gcaattagca
     8221 tattgttctg tcttatttgt taataaaata gattcagcag atgttgaaac aacgagcaaa
     8281 ctattgaaag atttagaagt tattaaccca gaggccgata tacaagtcgg tatgcatggc
     8341 agcgtcactt tgccaatatc agttagacaa atgacagcaa cttctgacaa taaacataag
     8401 tctttacatc aaatgattaa tcatcaattt gtgcaatcac cagtcaaatg tactaaagca
     8461 gagtttataa aacgtttagc atgccttccg tctcatattt ataggttgaa agggtttatg
     8521 acatttgaag acaccgcaca tacgtatctc attcaattta cacaaggaca atatgaatta
     8581 acacctgtag cattttcaaa aaaagtgcca gaatatttgg tgcttatcgg aaagggtatt
     8641 tcaaaagaag actatcaatg tttggaacag tagtgttttc agtggaagag aatggttaac
     8701 atgccttcat gtataataac gagttgattt gaacgtttaa gcgtaaataa aaataagctt
     8761 ggtcagccat caaatataat ttgaaaactg tccaagctgt tttattagag aacaatcaat
     8821 taaccccaca tatttaataa tacatcagca aagccttcag gtttttgaat ataacctaag
     8881 tgaccgcctg gaatatctac aataggtatg ccagtttctt tatttatata aaagttaaca
     8941 tcttgtggga aggagcctct agaatctgtc ccatttagta gggtgatttt atcgctgtat
     9001 tttgtgaaat catccaaagt aatatttgaa tgcgtatatt gtctaatttc aaattctaac
     9061 cagaacatcg tacgtttgta ctgttctata cgtccttctt cagtatcagc aggttgagac
     9121 atcatttttg catcaattgg tgcgatattt aatgtttcgc caaatgtttt catgcctttt
     9181 tctaagcctt ctgttaaaat ttgatgcaca atgtcatcat ttttatcttt ccaataagta
     9241 ctgtctggta aaaatgtatt aattggtggt tcgtgaaatg caatcttttt aacgacttca
     9301 gggtaatctt ttaacacatg catcgcaacg attgaacctg aacttgaacc taatatatag
     9361 acaggttcat cacttaatga ctttgcaagt tcggcaatgt cctgtgcgtc gcgtttgaca
     9421 cgataatcac tgtcagggtt tgaagcggaa tcagggagtg gttcagttaa ctcgctttct
     9481 ccataatcac gacgatcaac ggctacaaca gtaaaatggt cttttaactg ttctgcaaga
     9541 ggcagaaaaa tgtctccggt accgtttgca ccaggaataa agatgagcac gggtccttgt
     9601 ccgacttggt ggtatcgtaa tttagcgcct tgtaattcta aagtttccat attcaatgac
     9661 ctccatttgt taattgttag gtgataaacc taataattta gcaccatttg tataacttat
     9721 tttctctttt tcttcatctg ttaaacccag ttcatctaaa aatacaccta atttttcagg
     9781 ctcaatatat ggataatcag cagcataaag aattctatca atacctactt ctttcttgac
     9841 taaatcaaac tgtggcttcg ttaacatgcc actcggtgtg atataaaaat tatttttaaa
     9901 gtaatagctt acagggtggt tcaaatgttc agcgaataaa gcttcatcca tacgttctaa
     9961 gaagaatggg ataaactcac cccaatgtcc aataatcata tttaactttg gataacgatc
    10021 aaaaatacca gataatacta gatgtattgc atgaatgccg acatcaatgt gccaaccata
    10081 accaaaacaa gcaaatgttg ccgcagttac ttcaggataa tttcctttat agtatgattg
    10141 ataaatgtca ctgttaactg gcgcgggatg tagataaatc ggtacgtcta aattttcagc
    10201 tgttttgaaa ataatgtcat atttgtcttg atcaagaaaa ccatcttgtg cacgtcccat
    10261 aatgagcgca cctttgaatc ctaaatcatt gatgcaacgt tcgaattctc gcgctgcggc
    10321 ttcaggctca ttgataggta aagttgcaaa gcctacaaag cgattgggat attgtgcaat
    10381 atagtttgcc aattgatcat ttgctttttg acataattca atggctttct gaccaaccaa
    10441 atttgaagga gaaccatttc cataagataa gacttgaatt tgaacgtctt gattattcat
    10501 aaattggata cgttcatcat gatgtgataa ttcgtcggca tttgtaaaac ctgtcttttt
    10561 ttcaaggcct tctaacatta ctttcatcgg tacaccttta ggatctgctg atatcgcatt
    10621 catcgtttct ttttgaatat cttcaatgac ataatgttct tcaaacgtaa tacttttcat
    10681 ttacttcgcc tccatattgt attgcatgtt tattgcatct attgcagaag cattttttat
    10741 atacctctaa tttcaatgtt tgtaacataa aattgatcta ccaaggcatc tctccatcgc
    10801 cattaataaa tgtacctgtt gggccatctg caccaatcgt tgctaattga atgattggct
    10861 tgattccttc agaaacgtgt ttggaattat tactaaaatc accaactaaa tcagtatttg
    10921 tagcgcctgg atcagcagca ttgatttgca tgttaggtaa tcctttagcg tattgtagcg
    10981 ttagcattgt tactgccgat ttagacgaac aataagctaa tgaattcact ttagattcag
    11041 ctgtttcggg gtttgtaacc attccaaatg aacctaaacc acttgatacg ttgacgacaa
    11101 caggttgttc agatttttct aagagaggga cgaatgtatt catcattcgt acgataccga
    11161 atacattcgt ttgatatact tcttcaacgt cacgaggtgt caatttggaa ggtgctgaaa
    11221 attgaccaga tatacctgca ttgttaatga ggatatcaag acggccttct ttttcagcaa
    11281 tcatgttata agcatttttg actgagtagt cacttgtaac atctaattgt acataatgaa
    11341 cacctaattt ttgtgatgct tgttgtcctc ttacatcatt ccgagaacct atataaactt
    11401 tgtaacccaa tgctttaagt gcctctgcac ttgcatagcc taacccttta ttgcctcctg
    11461 tgattaacac aattttagtc attacgtccc acctcatcta aataaatgtt taataaataa
    11521 tttctgtacg cttcaattga aatatggcga tgctctattt ggaaggcaaa tacactagtt
    11581 gataatgatt gcaacagcat atctgttttg aacttcgtgt aagtgtcgtc atcgctttta
    11641 aataagtcat aataaaaatc aaataattct tgataaaatg cgctttggta aaaacgtaat
    11701 ttattgttgc ctgcttcaat acattgcagt agtgccttat tatcgatttt aaattgtaaa
    11761 agataatcta acgacacttg cataacctca taattagaat gatagtcatc tttaatttgc
    11821 ttaaaatgag tgataaaaat atcaaggtct ctttgtatga cgtagtagca taaatcgctt
    11881 ttatctttga aatgtcgata caatgtcccc ataccgatac ctagttcttt agcaatacga
    11941 ttcatactaa tgttttcaac gccttcttca tcaaaaagtt tgtgcgctat ttcttcaatt
    12001 cgttgcctat tctcttttgc atcttttcgc atgattacac ctacttaaaa ttctctaaaa
    12061 ttgacaaacg gataactctc cgtttattat aaaacgtgtt aagaaagtta gcaatgaatt
    12121 tgcaataact attaaatatc ataaaagaaa agagtgttga taatgtcttt aaataaagag
    12181 caaagacgca tcacagctga agagttgcaa gcacatttcg aagcatctac cttatcggtt
    12241 caaatgattg ctgaaaaact gaatgtcact acagaagatg tggaaaaagt attagctatg
    12301 acagcgccac taggcatttt tagtcatcaa ttacaacgat ttattcattt agtatgggat
    12361 gtcagagatg taataaacga caatattaaa ggaaatggac aaacaccaga accatatacg
    12421 tatttaaaag gtgaaaaaga ggactattgg tttttaagat aaaaagagga gtggatctaa
    12481 aatgaaagat attttagtaa taggcgcaac agggaagcaa ggtaatgcag ttgtaaaaca
    12541 gttgttagag gatggatggt atgtaagtgc attaactaga aataagaata atcgtaaact
    12601 ttcagacata ggacatccgc atttatcaat cgttgaaggt gatctgagtg ataatgttag
    12661 tttgcaatca gcgatgaaag gtaagtatgg cttatacagt attcagccaa tcgttaaaga
    12721 tgatgttagc gaagaattaa gacaaggtat gaagataatt gaaatagctg agcaagaaaa
    12781 tattcaacat attgtgtata gtactgcggg aggggttaat cgaaatcgaa ctggcccaca
    12841 ctttgaagta ctagcaaaaa tagaaaatag attaatggaa tctaatataa atgcaacagt
    12901 tatcaaacca tcattcttta tggataattt tttacgcatt gctaaagtcg aagatgaacg
    12961 tattacatta ccagaattta ttaatccgaa tataaaattc acaatgattt cttctattga
    13021 tatagctaaa attgcatcgt atatttttgc acatccacaa agctttacgc atcaatcaat
    13081 agagattggt tccgatgaag ttacattaag tgaagcagca acaattttta gtgaagtgac
    13141 aggtaaatct actgttatag aaggagaatt tgttagtggt gttgcagaaa agcaatggtt
    13201 ggaagaaaaa ggttatgaag tagactttga attaatggct gaaataaatc caacaagatt
    13261 atcattaagt gattggctta aagtccaaaa ctataataag taatgaatgg ggtgcaatca
    13321 tgattcaatc aatgtggttt aatttgcatg tgcaagattt agaaaagagc gcacagtttt
    13381 ataaagcgtt aggatttaaa ataaatagaa acccacaaat gttagataaa atggtcggca
    13441 ttcaaatagg tcaaacaacc gtaattttaa tagaaaacaa gcattttcaa aatgtaagtc
    13501 agcaaagcct taatactgaa ccaaatgaag tgatgatttc tctaggtgtg aacacaaatg
    13561 aagaagttga ccagttggtg aataaagtga aagaagcggg tggcacagtc gttcaagaac
    13621 caacagtaag ccaagggttt tatggagcaa tgttcaaaga tcttgatgga caccatttta
    13681 attttttggt ctgctaaaat gaaattctca aaagaataaa taaaaagccc atacagagat
    13741 gaagcgtaat gtgcaccatg gtgcgacaca ttagtgtctt caatcagtat gggctttgtt
    13801 tatgaggtca gttggcacct taagtgcaat acctcgtgag caaatattaa ttttgaatat
    13861 aaccgtattg ttggcgaaga tcataaactt tatctggtgt gacatcctga ccgattggat
    13921 caaccacttt tattgtggaa aatgttgtta atacttgtcc tcggatcatg cttaagtagt
    13981 cttgacgcaa tgcatgttgt tcttgttttt cagctacagt aagtggttgt actttttctt
    14041 tatttgcaag ttcattaatt ctatctaaaa ttttcatatt gtcatatcct ccttagaatc
    14101 aagtgttgat atattttgag attattttta tatggtaatt tatcaacaca ttaagtaatg
    14161 tttttagttt ctcggtcttt tgaccagcag atttttggaa ttaaaataag ggatttgaga
    14221 cacgatttta ctagtagtac gatggcaaag tgatgttatt gaagtgtcgt ttgatataca
    14281 tgtctgcaat gttgttttat aagacaggat gacgtaactg agcaaagaaa tgagcattgt
    14341 cgaatgacat cgtgtgtctt attaaaattg caatgggcat agcataggta tattcacagt
    14401 cgattttctt gatttctaca ttatcataat acgtagtgta aaagttatac gacagtcgat
    14461 agtcgattcg ctttttacca cgagcaatta ttatgtgttg cttcaacgtc gactttaatt
    14521 tatttgtaat gtatgtcact acacccagac tactctgatt aatatttaat atattatgcg
    14581 tgcgttcaaa cccctgaggt tcatctgtct cgctcacgtc aatccctcct atcttagtat
    14641 ttaaacaacg catatcaact atgcaacata tgttgtttag acatactata ctactatgtc
    14701 gtacgtttgc caataaacac gcacatatca tttgttgcct aaacaacata ttgcctgaag
    14761 tgttgtttaa gtacgcaaaa acaaaaatgc ttgtcactaa aatctatatg ttttaatgac
    14821 aagcttattt tcattcagtt aacgactgct aatatgacat tgtagagtat aggacgttga
    14881 tttatgtcct agacacggct aatcaatcgt aatacgttaa attttattgt gtgtacaagt
    14941 atgaacctaa gtcacatagt gatttcaaga tgcatgacta aataccaagt tttattaaaa
    15001 atgtgctata tgatgaatta aaaatggaag gttgcgatcg ataaacacat cggctgtctc
    15061 ttgactcgtg ttaaaccaat gtaatgctaa gcctgagata ccagctgcta aaaaactcgc
    15121 actatttaca atgatatctc gttgatgctc cggatagctg ttttctaaca taatcgcaaa
    15181 aatatcttct aattcattat taatacgttg atgtgctttg ttgcaaaatg cttcactatt
    15241 taattcgcaa gactttgcag catctttgat ataactcgca attgaaatga aaatattacg
    15301 cagaaccttt tcattaatga cattagaaat gctcaaatta tcattcaagt cttttaaaat
    15361 ggttacagat aatgtgtagt ctaggaggtc ttctttatca gtgaaatgag cgtaaaatgt
    15421 tgcacgattt agcgtagcaa tatcagtgat atctttaact gtaatttgag ataatttctt
    15481 ttctctagaa attttaagaa aagcatcgac aagcaattgt ttggttctaa cgattcttgg
    15541 atctatggat ttactttcca tcatttgttc actacctaac atctgttgtt ttaacactta
    15601 ttatcagtat cttctattat attgtcaatt gtgttgatga tatgtaagtg ctgatacgct
    15661 cctaacaaat aatgtacctt tttggtaagc atttgaatgt ctttcttcta ttgtattaat
    15721 ggattttaaa aatgaagata aaataaaagt gtattgtaac aatatgtttg aaaaattgcc
    15781 aatttcttta tctgaaaatt cagaattttg tgatatacta tttttcatag tcttgagtta
    15841 tagatgatac tgctagccta aatataacaa catcggcatg cacatcttag aatttaagct
    15901 agtggcagct tataacttaa ggctctttat tatttgcata cagatgtata tacataaatg
    15961 gaaggatgat attatgacat caattaaaac aattacaccc aaagatttta tatttagagt
    16021 tttatcaggt gtcgccatag gtattgttgc gggtttagtt cctaatgcga tacttggaga
    16081 aatttttaaa tactttatgg attatcatcc gatttttaaa acgttattag gcgttgtcgt
    16141 tgcaattcaa tttacagtac ctgctttgat tggtgcattg gttgccatga aatttgacct
    16201 atcgccatta gcaattgctg ttgtggcaag tgcagcatat gtaggaagcg gtgcagcgca
    16261 gtttaaaaat ggtgcttgga tgattacggg tattggtgat ttaattaata caatgattac
    16321 agcagcgatt gcagtgttat tcattttact tattcaacat cgcgtaggta gtatggcatt
    16381 gattgtattc ccaacagttg ttggcggtat ttcaggtgca attggtgttt taatattgcc
    16441 gtatacgaaa atgattacaa cagccatcgg taatatggtt aatggtttta cagaattaca
    16501 accgattgtc atgagtattt tgatttcaat ggtatttagt cttattatta tttcgccact
    16561 atcaactgtg gccattgcct ttgcgatagg tattacaggt ttagcagctg gatcagcgtc
    16621 aattggtatc tccgcgactg aagcagtatt gataattggt acaagtaaag tgaatcgttt
    16681 aggggtgccg ttatccgtct tctttggagg cgttaaaatg atgataccta atatggtgaa
    16741 atatccaatt cttatgttac caatattaac gacagccatt gtatcaggtt tggtatcagc
    16801 attagtaggt attcatggaa cgaaagagtc agctggattt ggttttatag gaatggtagg
    16861 accgattaat gcatttaaat ttatggaggt tgattctgct tggttgagtg tcttactaat
    16921 tgtagtagcg ttctttgttg taccatttgt aacagcttgg cttgcagata tcatttatcg
    16981 taaagtgttt cgtttatata ctaatgatat ttttaaattc atgggataat aacagtatgt
    17041 tttttgaaaa tatgagacca aaaattattt aatgttattg tgaattgcga gaatgaagtt
    17101 gaacaatata attgagctgc agtttatttg cgctaataaa agataagaca attcacaaaa
    17161 gatgtgtgtt ttaaaacttt gaatatacaa caataatagc aatcaataca tgtgtagaaa
    17221 catatttgtt gattgctttt taattgcgtt taatgtacga aaataagaga agttacatgt
    17281 cagatatatt acagctatg
//
LOCUS       DAMQYT010000031        17293 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMQYT010000031 DAMQYT010000000
VERSION     DAMQYT010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17293
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.031"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..1735)
                     /gene="srrB"
                     /locus_tag="PGA33_000858"
     CDS             complement(<1..1735)
                     /gene="srrB"
                     /locus_tag="PGA33_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HDG6472687.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIK"
     gene            complement(1716..2441)
                     /gene="srrA"
                     /locus_tag="PGA33_000859"
     CDS             complement(1716..2441)
                     /gene="srrA"
                     /locus_tag="PGA33_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HDG6472688.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            complement(2574..3311)
                     /locus_tag="PGA33_000860"
     CDS             complement(2574..3311)
                     /locus_tag="PGA33_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDG6472689.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            complement(3304..3846)
                     /gene="scpB"
                     /locus_tag="PGA33_000861"
     CDS             complement(3304..3846)
                     /gene="scpB"
                     /locus_tag="PGA33_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDG6472690.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKDQLVELIENYSS
                     HGLMIQRFGMTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            complement(3839..4570)
                     /locus_tag="PGA33_000862"
     CDS             complement(3839..4570)
                     /locus_tag="PGA33_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDG6472691.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            4662..5168
                     /locus_tag="PGA33_000863"
     CDS             4662..5168
                     /locus_tag="PGA33_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HDG6472692.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPVFERIIKANYPDYDYNQETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            complement(5246..6133)
                     /gene="xerD"
                     /locus_tag="PGA33_000864"
     CDS             complement(5246..6133)
                     /gene="xerD"
                     /locus_tag="PGA33_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDG6472693.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(6182..6631)
                     /locus_tag="PGA33_000865"
     CDS             complement(6182..6631)
                     /locus_tag="PGA33_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDG6472694.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            complement(6736..7278)
                     /locus_tag="PGA33_000866"
     CDS             complement(6736..7278)
                     /locus_tag="PGA33_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDG6472695.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            7360..8268
                     /locus_tag="PGA33_000867"
     CDS             7360..8268
                     /locus_tag="PGA33_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDG6472696.1"
                     /translation="MQKNILKSGISLSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIFIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            complement(8484..8732)
                     /locus_tag="PGA33_000868"
     CDS             complement(8484..8732)
                     /locus_tag="PGA33_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472697.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILGDISVDQISDGTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(8748..9503)
                     /locus_tag="PGA33_000869"
     CDS             complement(8748..9503)
                     /locus_tag="PGA33_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDG6472698.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDLKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGEIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            9645..10460
                     /gene="proC"
                     /locus_tag="PGA33_000870"
     CDS             9645..10460
                     /gene="proC"
                     /locus_tag="PGA33_000870"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDG6472699.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ"
     gene            complement(10565..11485)
                     /gene="rnz"
                     /locus_tag="PGA33_000871"
     CDS             complement(10565..11485)
                     /gene="rnz"
                     /locus_tag="PGA33_000871"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDG6472700.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKI"
     gene            11798..13282
                     /locus_tag="PGA33_000872"
     CDS             11798..13282
                     /locus_tag="PGA33_000872"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDG6472701.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
     gene            13512..14378
                     /locus_tag="PGA33_000873"
     CDS             13512..14378
                     /locus_tag="PGA33_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDG6472702.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(14461..16110)
                     /locus_tag="PGA33_000874"
     CDS             complement(14461..16110)
                     /locus_tag="PGA33_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDG6472703.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDFG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(16126..17145)
                     /locus_tag="PGA33_000875"
     CDS             complement(16126..17145)
                     /locus_tag="PGA33_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDG6472704.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETIASQNKINYQI
                     IETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTCIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
BASE COUNT         5570 a         3122 c         2525 g         6076 t
ORIGIN      
        1 gtttaataat aaatgttgtg cctttcccta attcgctttt aacatctatg gaaccaccat
       61 gctcttcgat aatcatttta caaatgaaca aacctaaacc ggtaccttgt ttacctcgcg
      121 ttctcgctgc atcaacttta taaaaacgat caaatacttg ttgtaaatgt tctggtgcaa
      181 tgcctgtacc tgtatcttta atgtataaaa tatcttcgct ttcattttca tcacaagtaa
      241 ttgcaatttc atctccaggt ttcgtataac gtgatgcatt atcaattaag ttcgttagta
      301 cttggtccat gcgatccata tcataactcc aaacacgctt cttacaataa ttaaaagtca
      361 tatttagacc taaatcatca gcttgttggc gatacttaat tttcatctta tctagtaacg
      421 ctgcaatagg ctgaacttct ttatttacgg ataacccttc agcatccatg cgtgcgacat
      481 ttaacaattc attaactaaa cgatttaaac gtttcgattc atcaaggaca atggcaagcg
      541 attcttttat ttcatccggt tctgtaacaa taccatctac aattgattca gtataacctt
      601 gaagtaatga tatcggtgta cgtaattcat gtgatacatt agcaatgaaa tctttcttca
      661 tttgatctag attgtgctca ttagtcatat cacgaactgt cacaacaaca ccacttttac
      721 ctccctgttc aatcttgtcg atatagcttg tggtcacaac aaagaatcgt gcattcattt
      781 ctaaatcgcg catttcagtt tgttttgatt taaaagtatc ttctatttgt cttaataaga
      841 aagctttagc atcttcatca atattgtcca taatatcatt cgccatctta ttagataaga
      901 taatttgtcg actctcatta atacctagga caccttctac catagagtta attaagctgt
      961 ctctaatatt tttagatgtg gataatgcgt cgacatgctc ttcgatttct gtactcatct
     1021 gattaaatgc ctgtgataat tgaccaattt catctttcgt tgtgacagaa ggtttataag
     1081 agtaatcccc ttcagataca cgtgtagctt ggtctcttaa acgtcttaaa ggttttgtaa
     1141 ttcttgacga taagaaaaac gcaaagactg ttgtaattgt taagaaaata acagccgtaa
     1201 ttatggtgat aatcgtaata gcattatttg tatcttcgat tgatttcaag tctttatata
     1261 taaagactcc actatatttg ctatgactat tcttctgtgc ttttgttgga tagcctaaca
     1321 aaatatatgt ttgagatgag cccttttctt taatcgttac atttcgagta acagatttac
     1381 ctttatcaaa cacatcgtca aaatggtcgt tgttgactac ttcattcaac atttgctttt
     1441 taatattaga aagtgaagcc gttgattgac gatgtttatt atttataatc atcaacccac
     1501 caggattttc aattaatgtt tgactatatt ttattgcttc ttctttatta tgtgattgtt
     1561 cgaccagtga acttatacgt ctagcatctt ctcttatggc attttcggtt tcttgtgtga
     1621 aatagtattg cataaaggta attaaagcaa tacttaataa aattaaaact gtcgtcacta
     1681 ttaaaataat agttaaccac agtttaatta cgacactatt tagccggctc atcattagat
     1741 ttaacctcaa atttataccc aacgccccag actgtttgaa tcatatgcgc agcttcacta
     1801 gacacacgat ttaacttttc tctaagtcgt ttaacatgag tatcaactgt tcttaaatca
     1861 ccatagaatt cataatgcca aacttctttt aataattgtt cacggtcaaa tactttattt
     1921 ggtgttttag ctaaatatat taataattcg tactctttag gagtcaaatt aacttcttga
     1981 ttatcagcaa gtacgcgatg tgcatcatta tctatttcta aatgtttaaa ttcaatcaca
     2041 tcacgtgcgt gaggttcgct ttgttctaca gttgtagatt gcgttcttct tagaagtgct
     2101 ttaactctta agactacttc tcttggtgaa aatggtttga cgatataatc atctgcacca
     2161 gattcaaaac cttcaacacg gtttgtttct tcacctttag cagtcaacat aataatcggt
     2221 gtttgtttat gttcacgcaa tttagttgcc acctggatac catccatttc aggcaacatt
     2281 aaatctagta gtatgcaagc ataattattc tccattgcaa gttcataagc ctcttggcca
     2341 ttacttgctt catggatttc aaaagattct ctttctaaat acattttaag taatcttctg
     2401 attctatcct catcatctac gataagtatt tcgttcgaca tacaggtcat acctcccaca
     2461 catgcttttc tttacaaaag tattatatca caggaaagcg catacagtta ttaaaaacct
     2521 aaaattttgt gaaaaaattc tcaaactttg tgataaatac atattttatt taattatttc
     2581 ccatgttctg ctaaatgacg catcactttt acttcatgag gcgtcaatac acgtccttca
     2641 ccagcattca aaccgacaac atttaaaggc ccatattcaa tacgagacag tttcgtcact
     2701 tgatgaccaa aatgttcgaa cattcttctg acttggcgat tacgaccttc tgtaattgta
     2761 atttcaacca atgttgtgtt tttatcttta tcttgtttct taactttcac ttcagccggt
     2821 tgcgtcatac catcttctaa ttcaatacct ttttctagcg ctttcacttc ttctctcatt
     2881 aaataacctt ttaatttcgc aacatatttt ttcttaattt gatatcttgg atgtgtcatt
     2941 aaattagtaa attccccatc atttgtgagt aataacaatc cagaagtatc atagtctaaa
     3001 cgaccaacag gataaatacg tgcttctata tctttaaagt aatctgtaac cactgtacgt
     3061 cctctatcat cagatacact tgttatcact tgagttggtt tatggaataa aatgtaaatt
     3121 ttgtcttcta gttctatttt aataccttca acttcaatcg tatctgatgg cttcactttt
     3181 gttcctaatt cagtgacagt cgtaccattc actttcactt ttccttcaga aattaaagtt
     3241 tctgccttac gtcttgaagt ataaccgcta ttagctatac gtttttgtaa tcgttctaat
     3301 tctttagtca ttattttctc ctttttgatt gactagatta ctgaaaaaag catccatttc
     3361 ttcatcgtct tcttcagttg tcggcaaatc ttctatattg gaaataccaa atacatttaa
     3421 aaataaatca gtagtaatta actgttggct acgctgttca ttaaccactt tagcctcaac
     3481 tagtcctttg gcaatcaatg tcttaactgc accatctgaa ttgatactac gaattaattc
     3541 aatatcactt cttgataatg gctggttata agcaataatt gatagtactt ccattgctgc
     3601 ttgtgataat ttcatttgtg acttttgttc aattaattgt tcaatatacg ttgccgcttc
     3661 ttttttagtc gttaaaacat acgtcattcc aaatcgttgt atcattaatc catgtgatga
     3721 ataattttca attaattcaa cgagttggtc tttcgacata tctaatattt ctaatagttg
     3781 tttttcatct aaaccttcat cgccagctgt aaataaaagc gactctaata taccatgatt
     3841 atccaaaatg gtagttcact cctctaataa tgttaatatc ttcaaaatta cgttgctgct
     3901 caatattaat tattcctgct tttgacatct ctaaaatagc taaaaagtga gtgactactt
     3961 gttcaattgg ctcagaaaac gtaaacagac taaagaagtt aaaatgatct ttatctttca
     4021 atctcgatgt cacttgttct gtagcttgtt gaatggtaaa tgtctctttt cggatttcaa
     4081 cagatttagg tgtatttaac tcaactctat ttttaactct ttgataagct acaattaatt
     4141 cagttaaatc aatcgtatga tttggatccc aagattcatc tgtttccaaa tgagataaat
     4201 ctgtcggtct ttttgtaaaa taaaaatctc tttcttcttt catgtcattt aaaatagcag
     4261 tatattcttt ataattttga tattctatta aacgcccaac taaatcttcc cgtgggtcat
     4321 catcaacatc catatctgat gttgattgtg gtaatagcat cttactttta atcattaaga
     4381 gttctgacgc taatactagg tattcacttg caatattaat ttcaagctgt ttcattgcat
     4441 gaacgtactg catatactgc tctgttaatg cttgcatagg aatatcataa atatctattt
     4501 caaatttttg gataagatgc agcaataaat ctaatggtcc attgaaagca tctaatttaa
     4561 cttcatacat aatatctacc tcgtattgcg tcatatgtgc ataattctct aaaatattat
     4621 agcatgaaaa taacttactt ttaaattggg gtgataacaa aatgcaacag gcactgataa
     4681 atttctatta tcaatttcat actaagcaac attatttcct atgtcatgac attttagaag
     4741 atgcatggaa agctgaaaat aactacagta agcaagatgc agttgttagt ttaatcttgt
     4801 ttgcaactgc ttgttaccat tatcgccgca ataatttaaa aggcgcctat aagtctttta
     4861 ataaatctaa agaaataata caaaatgcta aagatagaga tgcgttatac cttaatttaa
     4921 atgattatca gttgttaatt gagcaacaga ttgcaaaatt gaatgcagca aagccttttt
     4981 catctgttat attaccaata actccagtct ttgaacgcat catcaaggcc aactatccag
     5041 actatgatta taatcaggaa acagcaacag atccttttat tgtagaccat cacatgcgcc
     5101 gtgatcgttc agaagtgatt gcagcaaaag aagaagcgat tcaattaaga aaacatagac
     5161 gtaattaatc aaccaattta aaaaagccac aatagcacag acatttttag tcatgtgtat
     5221 tgtgactttt gagttattgt tcactttatg ctctaggatg aaattggtta tacatttttc
     5281 taatttgaga tttcgaaaca tgtgtataga gttgggtagt agatatgtca gagtgaccta
     5341 acatctcttg cactgctctt aaatctgcgc cattttccaa taaatgtgtc gcaaaagagt
     5401 ggcgtaacgt atgtggcgtt aacgtctttt taatgtttgc ctttacacca ttttgtttaa
     5461 tcattttcca tattgcttgt cgtgataaag gtttaccatg catatttaaa aataagactt
     5521 cagtaacagt cttttttaaa agttgcggtc taatcgtttc aatataagta gttaagtact
     5581 caatgactgc gtcgcctaat ggtacaattc tttctttatc gcctttacca aatacgcgta
     5641 caaatcccat tattaagttc acgttttcta actctaaatg tatcaattca gatacacgca
     5701 ttcccgttgc gtacagaagt tctaacatcg tacgatcacg atatccatta attttattta
     5761 aatctggcgt ttctaataaa gccaacactt cgtcaacatt taaaacgtca ggcaattttt
     5821 tgtcatattt tggtgaatct aataataccg ttggatcttt cgccgcatat ttttctctta
     5881 tagcaaattg atgaaaacta cggattgttg aaataaatcg cgcaatagat ttagcagatt
     5941 gcccttggtc tattaaatgc cccaaacact cttgaattaa ttgtcgatct ataaaatcaa
     6001 tatgcgagat atgatgttca gtcatataat cttgatactt tttcaaatca cgtctataag
     6061 caccaattgt gttagaactt agtccttttt caatttgtat aaaacgcaaa tattcttcaa
     6121 taattgtttc cataaccaca cctctaaaaa aagcgaaggt taatttaacc tacgcaattt
     6181 actatccttt acctttagct tggcacgttt cacacacacc atggaaagtt aaacgatgat
     6241 ctaaaatttt aaaattgaac tcattttcaa ctcgattttc aacttctggt aacaaatctt
     6301 catcgatttc atctacacga ccacattcca tacatactaa atgatggtgg aaatgttttg
     6361 cgccttcttt tcttaaatca aaacgagcga cgccatcacc aaagttaatt ttgtcgacaa
     6421 cttttagttc agctaacaac tctaacgttc tgtatactgt cgccaagcca atttcaggcg
     6481 ctttatcttt tactttcaga tatacgtctt cagcacttag atgatctttt tcattttcaa
     6541 ttagaactct aacagtagct tcgcgttgtg gcgttagctt atatgatgat tgttgtaatt
     6601 gttgcttaac gcgatttaat cgttcttcca acgatgtcca ctcccctact aataattaaa
     6661 atcattacaa attatttcaa acttcacaat taaaactaac agttttctca ataaaatgca
     6721 agcttttctc atttgttatt tagaatgatt ataatttaat aataggtgtt gcaatgcaat
     6781 gatagtcttc gcatcttcta tttctttatt cattagcatc gatttaacat tctcaatcgg
     6841 aactttaatg acttcgacaa attcatcttc atctaagtga actgtacctt cttctaaatt
     6901 atccgtaaaa tatattgata attgttcatc gcaaaaacct ggtgaaccat acatatctac
     6961 aacatgtgtt aactctttgg caatatatcc tgtttcttct tctaattcac gttttgccgc
     7021 ttcgactcta tcttcatcat cttctaattt accagctgga atttctagca atggtttttc
     7081 tactggttta cgatactgtt tcactaatac gacttctttt ttaggtgtca ctgcacaaac
     7141 agcaactgca ccattatgat aaactaattc tcttgttgac gtttcaccgt ttggtaatgt
     7201 cactgtatga atttctacat ctacaatttt gccattataa ataactgttc gatcaattgt
     7261 tttttcattt aaatccatta taatcacgtt cctttaaatt catattatat attgatacac
     7321 tatgcttgtt agctaaagtg tatcgaaagg agaacagaca tgcaaaaaaa tatattaaaa
     7381 agtggtattt cattatctga attaggtcta ggttgcatga gtttaggtac agatttaaaa
     7441 aaagccgaac aaattataga ttgtgctgtt gaaaatggta tcacttattt tgatacagca
     7501 gatatgtacg ataaaggtat aaatgaatca gttgttggta aggcacttct taaatatcaa
     7561 caacgcgatg atatttttat tggtacaaaa gtaggcaatc gtttaacaaa agatggcagt
     7621 acaacatggg atccgagtaa atcctatatt aaagaggcag ttaaaggttc actaaagcgt
     7681 ttaggtatcg atcatatcga tttatatcaa cttcatggcg gaaccattga tgacccatta
     7741 gacgaaacaa taagcgcatt tgatgaattg aaacaagaag gaattatacg tgcttacggt
     7801 atttcttcta ttcgcccaaa tgtaattgat tattatttaa aacatagtca aatcgaaacg
     7861 ataatgtctc aattcaattt gattgataat cgtccagaat cattattaga tgcaattcac
     7921 aacaatgatg ttaaagtatt ggcaagagga cctgtgtcta aaggattatt aacttcaaac
     7981 agtgttaatg tgctcgacaa taaatttaaa gatggtattt ttgattattc tcatgatgaa
     8041 ttgggtgaaa caatagcctc tattaaagaa attgaaagta atttatctgc attgacattt
     8101 agttatttaa catcacatga cgtgcttggt tccatcattg taggtgcaag tagcgtcgac
     8161 caattaaaag aaaatattga aaactatcat actaaagtta gtttagatca gattaaaaca
     8221 gcaagagctc gtgtaaagga tttggaatat accaatcatt tagtgtagaa gtcattttca
     8281 gtaataaaaa cagcagcatg aggcgtttca ttataaaaat gccttactgc tgttgtttat
     8341 gtacaattcg ctataattta tgattatgat tactcactta tgatagaaat taaagcgttg
     8401 tcctcacgca tcagtattta gtaatttcgc cttgcggcat tgccttaagc aaacttctgc
     8461 cacttcatct cttaataatt ttattaaaac atctttctat atttcacttc gcatgttgat
     8521 tcatcattat tagttattat ttgtacaccc agcacatttc cttgcaacac aagtagtttg
     8581 aatttttcac aagtataata taatgtaccg tctgaaattt ggtctacaga aatatcgcct
     8641 aaaatatcca gcactgtaaa ttcttcaaat actgatagtt gttccgcata tcgtacacaa
     8701 agtcttacca cactctccga ttgacagttc attgccatcc cacctattta tgctttattt
     8761 ttaaataatt tagggaaaca tcgttcaaaa aatctaggcg caatttgata cattttcaac
     8821 gcatgatgca tccatttagg ccgattaatt tccaattgtt ttgttttaat gccataaatg
     8881 atatcttctg caagctgatt agcatcaagc ataatttccc ccatcttttt agcatacttc
     8941 attgatgggt cggctttttg atgaaaaggt gtatcaatcg ggccaacatt aactgtcatg
     9001 atatgtaagt ttggtgactc tagtcttaaa gcattcatta atgcataaaa ccctgctttc
     9061 gatgccccat aatgtgcagc atttgcttgt gtggaaaatg cagcttgact tgaaatacct
     9121 acaatatgtg cgttagatgt taaatatggt ctcaacacag tatataaaac attaaaacta
     9181 attaaattaa gctgatacgt ttcaatcatt tctgaaaaac tatggtctga aatagatttg
     9241 aaataaccta aacctgcact ataaatgaat ccatcgaatg atgtattgtc ttcaaattgc
     9301 agtgcctgta tcgacttcaa atcatttaag tcacaaggaa taacatttat agttttcccc
     9361 aattcctgtt caaagattct agttgcttta tcaacatcac gcaccaacaa cgttacatgc
     9421 actttatttt ctagtaactt tcggacaatc gataaaccta aaccactcgt accaccagtc
     9481 actataaaat gttgtccttt catcaattaa ccttcctttt caattatata gaatgcaatt
     9541 tatcaacttt acataattga gacaagttga ttatctttcc taatatatat acaataataa
     9601 gaaaatataa catacaaatc aaaaactaaa gggatgtgac gttaatgaaa ctcgtatttt
     9661 atggagctgg taatatggca caagctatat ttacaggaat tattaactca agcaacttag
     9721 atgccaatga tatatattta acaaataaat ctaatgaaca agctttaaaa gcattcgctg
     9781 aaaaactagg tgttaactat agttatgatg atgcgacatt attaaaagat gcagattatg
     9841 tatttttagg taccaaacca catgattttg atgctctagc aacacgcatc aaaccacata
     9901 ttacaaaaga caattgcttc atttcaatta tggcaggtat tccgattgat tatattaaac
     9961 aacaattaga atgccaaaat ccagttgcta gaattatgcc aaacacaaat gcgcaagttg
    10021 gacactctgt tactggcatt agtttttcaa acaactttga ccctaaatct aaagatgaaa
    10081 ttaacgattt agttaaagca tttggttctg taattgaagt atcagaagat catttacatc
    10141 aagtaacagc tatcaccgga agcggcccag catttttata tcatgtattc gagcaatatg
    10201 ttaaagctgg tacgaaactt ggtctagaaa aagaacaagt tgaagaatct atacgcaacc
    10261 ttattatagg tacaagtaag atgattgaac gttcagattt gagcatggct caattaagaa
    10321 aaaatattac ctctaaaggt ggtacgacac aagctggcct tgatacattg tcacaatatg
    10381 atttagtatc tattttcgaa gattgtctaa acgctgccgt cgaccgtagt attgaacttt
    10441 ctaatataga agaccaataa aaacaaaccc gccaacacat gtatgcatca tcgcaagcac
    10501 tgtgtttgac gggttatttt tataatttat tgttatttgg caagcattgt ttattacttt
    10561 gtcattagat tttaaaacta tcaaaatctt ttacaaaatt aaaattaggt gtatcttcat
    10621 tttgtatcaa tgtttgataa atttcattta tatcttctgt attatagcga ttgctcaaat
    10681 gtgtaatcaa cgtacgttta acattggctt cttttatcaa tgcaaatacg tcttcaatat
    10741 ggctatgatg ataattgttg gctaaatgct tttcaccatc tatataggtc gcttcatgta
    10801 ccatcacatc agcatctcta gaaatcacac gttcattaga acatggtttt gtatcaccaa
    10861 aaattgctac aactggaccc tgtttggact cacctctaaa atcttttgat tgataaactt
    10921 gaccattatg ttcaaatgta tcatgagatt ttacttcttg atatttagga cctggttcaa
    10981 gaccaatgtt ttttaacgct tcaacattga ttgtacctgt agtttcaggt gccattactc
    11041 tatatccata tgatggaata ccatgattaa gtaaatgcgc ctctacagta aaaccatcat
    11101 gatgatatgt cagatgatca tcgatttcaa tatatgtaat tggatagttt aaatgtgact
    11161 ctgataaatt catagacatt tccacatatg ctttaattcc ttttggtcca accaatgtaa
    11221 gcggcttctg ttcaccgccc tgaaaagaac gactagaaag taatcctggc aaaccaaaaa
    11281 tatgatcgcc atgcatatga gtaataaata tatgtgtcac ttttcctaat ttaattgcat
    11341 gatgtaaaat ttggtgctgt gtaccttcac caacgtcgaa aagccatatg gaattggaat
    11401 atggttctaa atttaaggcg attgcttgtg tatttctctc ttttgtaggc aaacctgcac
    11461 tcgttccaaa aaatgtaact tccatatatg cccctccttt tcttcaattc attttatcat
    11521 aaaatttgta tcaatttagt tttaatttat acttgaatca aaatctgtta aaaattcgtc
    11581 tttgctagtt aatttttcta actttaacgt agacataact atataaattt tgataattac
    11641 gttatactta tcattaataa gtatcacatt aaacatgata catgaatcga tatttcattt
    11701 aagacactgc atacagtcga gcatattgta tgacctactg aatggattat cttataataa
    11761 taaatcatat atctaattaa gaattgaggt tttaatcttg agtactaaaa acaaacacat
    11821 cccatgttta atcacaatct ttggtgcaac tggtgactta agccatcgta agttgtttcc
    11881 atcaatattc catctctacc aacaagacaa tttagatgaa catattgcca tcatcggtat
    11941 tggacgtcgt gacattacta atgatgattt ccgtaatcaa gtaaaatcat caattcaaaa
    12001 gcacgtaaaa gatacaaaca aaattgacgc gtttatggaa catgtcttct atcatagaca
    12061 tgatgttagt aatgaagaaa gctatcaaga attactagat tttagtaatg aattagatag
    12121 ccaatttgaa ttaaaaggta atcgactatt ctatttagca atggcaccac aattctttgg
    12181 cgttatttct gattatctaa aatcttctgg tcttactgat acaaaaggat ttaaacgcct
    12241 tgttatcgaa aaaccattcg gtagtgattt aaaatcagcc gaagcattaa acaatcaaat
    12301 tcgtaaatca tttaaagaag aagaaattta tcgtattgac cactatttag gaaaagacat
    12361 ggttcaaaat atcgaggtat tacgttttgc gaatgcgatg tttgaaccat tatggaataa
    12421 caaatatatt tcaaacatcc aagttacatc ttctgaaata ctaggtgttg aagatcgtgg
    12481 tggttattat gaatcaagtg gcgcgctaaa agatatggtg caaaaccaca tgttacaaat
    12541 ggttgcatta ttagctatgg aagcacctat tagtttaaat agtgaagata tccgtgctga
    12601 gaaagtaaaa gtacttaaat cactgcgtca tttccaatct gaagatgtta aaaagaactt
    12661 tgttcgtggt caatatggcg aaggctatat cgatggtaaa caagttaaag cataccgtga
    12721 tgaagatcgc gttgcagatg actctaacac acctaccttt gtttcaggta aattaacaat
    12781 tgataacttt agatgggctg gtgtaccatt ctatattcgt actggtaaac gtatgaaatc
    12841 taaaacaatt caagttgtcg ttgaatttaa agaagtacca atgaacttat actatgaaac
    12901 tgataaactg ttagattcaa acctattagt aatcaatatc caacctaatg aaggtgtatc
    12961 tttacatcta aatgctaaga aaaatacaca aggtatcgaa acagaacctg tccaattgtc
    13021 ttactcaatg agcgctcaag ataaaatgaa tactgtagat gcatatgaaa atctattatt
    13081 tgattgtctt aaaggtgatg ccactaactt cacgcactgg gaagaattaa aatcaacatg
    13141 gaaatttgtt gatgcaattc aagatgaatg gaatatggtt gatccagaat tccctaacta
    13201 tgaatcaggt actaatggtc cattagaaag tgatttacta cttgctcgtg atggtaacca
    13261 ttggtgggac gatattcaat aattgaatta aaacgcacat gttaaacaaa aataaatgag
    13321 cgaatgacaa ggattgatta cgaatcaagt aaatcaattg ttctatcatt cgcttttttt
    13381 ctagactata ttatgaaatt atattttaca atgcccaaaa ctattttaat aatcattgaa
    13441 caaatgggtg tataatttat agaaataatg tagaataaaa ataaatgatt gaattaattg
    13501 gagtgaaagt tttggacgtt atcaagcaaa tacaacaggc aattgtttat attgaagatc
    13561 gtttattaga gcctttcaat ttgcaagaat taagtgatta cgttggtctt tcgccatacc
    13621 atcttgatca atcatttaaa atgattgtcg gcttatctcc agaagcttat gcacgcgcgc
    13681 gtaaaatgac actcgctgca aatgatgtga ttaatggtgc tacacgactt gtagatatcg
    13741 ctaaaaaata tcactatgca aattcaaatg attttgcaaa tgattttagt gattttcacg
    13801 gcgtatcacc tattcaagcc tctactaaaa aagatgaatt acaaattcaa gagcgattat
    13861 atatcaaatt atcaactact gagagagcac cttatccata cagattagaa gagacagatg
    13921 atatttcatt ggttggatat gcacgattta tagacactaa gtatttgtca catcctttta
    13981 atgttccgga ttttttagaa gacttgctca ttgatggtaa aattaaagag ttacgacgat
    14041 ataatgacgt tagtccattt gaactatttg ttattagttg tcctcttgaa aatggtttag
    14101 aaatatttgt aggtgtacca agtgaacgtt atcctgcaca cttagaaagt cgatttttac
    14161 ctggcaaaca ttgtgcgaaa ttcaatttac aaggtgaaat tgattatgca actaatgaag
    14221 cttggtacta tattgaatca agtttgcagt taacattgcc atatgaacga aatgatttat
    14281 atgttgaagt gtaccctctc gatatttcat ttaatgaccc attcactaaa attcagcttt
    14341 ggattcctgt taaacagagt ccttatgacg aagattaaat aataaaaaac aaagaagccc
    14401 cctaatatat ctataggtct acaaatggcc ttagattcta ttagggggca tattaatatg
    14461 ttaatttagt tcgataacac atgcttcata tggacgtaac tgttttaaat taactttggc
    14521 atcataatta aatagcttta cttctccatg gcttaaatca aatggtacag ttaattctgc
    14581 ttcgtggtta gtaagattac ctacaataag aacttgcttt tcatttaatg ttctcgtgta
    14641 cgcaaaaact tgtgaatttt cagcatctac taaatcaaat tgaccatata cgtatacatc
    14701 attagacttt cttaattgaa ttaaatcttt ataaaattgt aatactgaat gctcatcttc
    14761 taattgttgt gcaacattga tagttttata attcggattc actgggaacc acggttcacc
    14821 atttgtaaat cctccattta acgtatcatc ccattgcatt ggtgtgcgag aattatctcg
    14881 gttctcatct ttatatttcg caagtaaagc gtctacatct ccaccttgag ctttcactat
    14941 ttgatagtca tttttaacag caacatcgtt aaacgtttca atactttcaa atggataatt
    15001 cgtcatacca atttcttgac cttgataaat gaatggcgta ccttgttgca agaaataaac
    15061 agctgcatga cttgttgctg attcatacca atacttgtca tcgtcacccc acgtcgatac
    15121 acgtcgtggt tggtcgtgat tttcaataaa caacgcattc caacctttat tttcaagttg
    15181 tttttgccat ctatttaata cagatttata cgaatttaca tcaaagtgag aatcaccact
    15241 attccacagt cccaaatgtt caaattggaa tatcatatta aatttaccat tttcttcccc
    15301 gacccagtca tcagcatcat cagggcttac accattcgct tcaccaacag tcataatgtc
    15361 atacttactt aatgagcgat ctttcatctc ttgtaaccaa gtttgtatac ctggctgatt
    15421 catatctaca tcaaatgctg gggcatatgt tttaccctca ggtacaggta agtcacccgc
    15481 ttcaaacgtc ttcttaatat gcgtaattgc atctactcta aatccatcaa tgcctttatc
    15541 aaaccaccag ttcatcattt caaatacagc atctctaact tccggattac cccaattcaa
    15601 atcaggttgt tttttactga ataaatggaa ataatattgc tcagtattag catcatattc
    15661 ccatgtagat ccattaaata tactttccca gttgttaggt tcagagccat ctggctttgg
    15721 atcttgccaa atgtaccaat cacgtttggg attgtcttta ctagatttgg attctataaa
    15781 ccaaggatgt tcatcagatg tatgatttac aactaaatct aaaataagct tcatgcctct
    15841 atcatgaaca ccttttaata aacgatcaaa gtcttccatc gttccaaatt catccataat
    15901 ctcttggtag tcactaatat cataaccatt gtcatcatta ggtgatttaa acattggact
    15961 gagccaaatg acatcgatac cgaaatcttt taagtagtcc aatttatcaa tcattccagg
    16021 taaatcccca ataccatcgt gattactatc attaaaactt cttggatata cttgatatgc
    16081 tactgcttct ttccaccatt gcttattcat tttaaaactc ctttgctatc gctgtgttga
    16141 ttttcttatt tttaattctg tatctataat gacgagttca ataacatcct gtgctttgtt
    16201 tttcaatata tttaaaattg ctgcaccagc ctgttgacct aacattcgag gcttgatgtc
    16261 aatacaggtt tgtggtggtg acgcaatttc ggttaaataa gaatcattga acgttgctgt
    16321 cattacatct ttcggaattt caatattaag ttcatatagg acacttaaaa tcgctaaatg
    16381 taacatagca tctaacgaaa tgattgcctg tttaatattt gggtccttca aacgcgtatg
    16441 tagattttgc atgtaattaa aaataacttc tctttcatta ctagtctcaa taatttgata
    16501 attaatttta ttttgagaag ctatcgtttc aaatccttga attctatctt ttgaaacttc
    16561 aaaatttcct ttttctgtaa taaatattaa ttcatctaca ccttgttcaa taacatgtcg
    16621 tgtcaaattt tcagaagcta atatattatc attatctata tgtgtaaatt gatgatctat
    16681 atccgatgta ggcttaccaa tcacaataaa tggcatgctt tcatcaatta acatttgttt
    16741 aatcggatca ttttcttttg aatagagcag tataaacgca tcaaccattc gttgtttaat
    16801 cattttataa acttcatcca ttaaatcatt catattattt gagactgtcg tttgtgtacc
    16861 atagccatgc tggttacacg tttcagaaat tcctagcaat acattgatgt agaatggatt
    16921 cagtcgaata ggctcctcag acccttttaa cactaaacca attttatatg tttggtttgt
    16981 aattaaagtt ctagcagcgg tattaggaaa ataattcaat tcttccataa ctttcttcac
    17041 ttttgaaatt gtcgcttcgc taatacgttg atttcctttt ataactcttg aaactgtcga
    17101 aggagaaaca ccggctttta gtgcaacatc tttaatcgta accatttaat cacctcctgt
    17161 taatttctgc attggaaaac gcttccaacc actgtataat accagtttag tcacactttc
    17221 taaaaaagtc aaaagatttg tgcaaacgat tgcataaaac gataaaaata aaaccttcat
    17281 actgaaattc aat
//
LOCUS       DAMQYT010000032        17205 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMQYT010000032 DAMQYT010000000
VERSION     DAMQYT010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17205
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.032"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(683..1543)
                     /gene="folD"
                     /locus_tag="PGA33_000876"
     CDS             complement(683..1543)
                     /gene="folD"
                     /locus_tag="PGA33_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDG6472705.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            1744..2226
                     /gene="purE"
                     /locus_tag="PGA33_000877"
     CDS             1744..2226
                     /gene="purE"
                     /locus_tag="PGA33_000877"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDG6472706.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            2213..3337
                     /gene="purK"
                     /locus_tag="PGA33_000878"
     CDS             2213..3337
                     /gene="purK"
                     /locus_tag="PGA33_000878"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDG6472707.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            3341..4045
                     /locus_tag="PGA33_000879"
     CDS             3341..4045
                     /locus_tag="PGA33_000879"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDG6472708.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLK
                     QLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            4045..4308
                     /gene="purS"
                     /locus_tag="PGA33_000880"
     CDS             4045..4308
                     /gene="purS"
                     /locus_tag="PGA33_000880"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDG6472709.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            4310..4981
                     /gene="purQ"
                     /locus_tag="PGA33_000881"
     CDS             4310..4981
                     /gene="purQ"
                     /locus_tag="PGA33_000881"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HDG6472710.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLATEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            4974..7163
                     /gene="purL"
                     /locus_tag="PGA33_000882"
     CDS             4974..7163
                     /gene="purL"
                     /locus_tag="PGA33_000882"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDG6472711.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            7142..8626
                     /locus_tag="PGA33_000883"
     CDS             7142..8626
                     /locus_tag="PGA33_000883"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDG6472712.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            8619..9647
                     /locus_tag="PGA33_000884"
     CDS             8619..9647
                     /locus_tag="PGA33_000884"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDG6472713.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDVSRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            9650..10216
                     /gene="purN"
                     /locus_tag="PGA33_000885"
     CDS             9650..10216
                     /gene="purN"
                     /locus_tag="PGA33_000885"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDG6472714.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            10231..11709
                     /gene="purH"
                     /locus_tag="PGA33_000886"
     CDS             10231..11709
                     /gene="purH"
                     /locus_tag="PGA33_000886"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDG6472715.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPATVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPSGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            11731..12978
                     /locus_tag="PGA33_000887"
     CDS             11731..12978
                     /locus_tag="PGA33_000887"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDG6472716.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESDHQAILDFAKRQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYKK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(13243..14049)
                     /locus_tag="PGA33_000888"
     CDS             complement(13243..14049)
                     /locus_tag="PGA33_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDG6472717.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG"
     gene            complement(14042..15442)
                     /locus_tag="PGA33_000889"
     CDS             complement(14042..15442)
                     /locus_tag="PGA33_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6472718.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQLP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(15457..16032)
                     /locus_tag="PGA33_000890"
     CDS             complement(15457..16032)
                     /locus_tag="PGA33_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDG6472719.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(16109..16209)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(16616..16750)
                     /gene="graF"
                     /locus_tag="PGA33_000891"
     CDS             complement(16616..16750)
                     /gene="graF"
                     /locus_tag="PGA33_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HDG6472720.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     gene            16902..>17205
                     /locus_tag="PGA33_000892"
     CDS             16902..>17205
                     /locus_tag="PGA33_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472721.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGNFPIVSAIVIIIFGILVMLIGHTLLRTIKYD"
BASE COUNT         5973 a         2554 c         3259 g         5419 t
ORIGIN      
        1 ttataaataa tgaggaaata attattattt tgaaaatctg ccacaattgt aaaattaata
       61 atttagggat aatttgaaat caaatataac tttattcgac agtttgaact taaatttttg
      121 tgtatcaaaa caataagaaa agggaggcga aaatgataaa gaaccactaa tgattaacta
      181 tgtagtagta ctttatcata gtcacagctt ctgtgttggg gcccatcccc aacttgcaca
      241 ttattgtaag ctgacttttc gtcagcttct ttgttggggc cctgactaga attgaaaact
      301 tgttacaagc ttattttcat tcaattaact actgtctata taaaattgtt gagccaagta
      361 caaagtgaaa tgctcccttc aaagccttca tttattcaat gtctactttg aagggagcat
      421 ttgatcgatt aatgtcccag atttttcttg ttattcattg caattgcaaa ataatattta
      481 atgtgggtta aaagacttta ttaaaactca gttattaggg agtgggacag aaatgatatt
      541 ttcgtaaaat ttatttcgtc gtcccacccc ggcaaggttg actagaattg aaaaaagctt
      601 gttacaagcg aattttcgtt cagtcaacta ctgccattat aactttgtag agcatagaac
      661 attgatttat gtctcaggct ctttacgaat caatacctcg acgcattttt tctgcaagca
      721 aagtattatt taataccata gtaattgtta atggaccaac gccaccagga actggtgtaa
      781 tagctccagc aatttcttta accgcatcat aatcaacgtc accttttaat ttgccatttt
      841 catctggcgt attgccaaca tcgataatta ctgctccttc tttgaccaca tcttttgtta
      901 ctaaaccagg cttaccaact gcactgacaa tgacatcagc atcttttaaa tatgatgcca
      961 tatcttttga acgagaatgt aagattgtta ctgatgcatt tttttgaagt agtaacttag
     1021 aaactggttg tccgacaata tgacttcgtc caattacaac tgcattttta ccttctaaat
     1081 caatatcagc atgttttaat atttccatga tgccgagcgg tgtgcaaggt acaaaagttt
     1141 gttcatcgat atataatttc cctatattta ttggatgaaa accgtccaca tctttttcag
     1201 gattgattgc ttctaatatt ttctgttcgc taacttgttt tggtaatggt acttgtacca
     1261 aaataccact tacagaatca tcattattta gtctatttag ttcgtttaat acttcttctt
     1321 cagtagctgt ttcttccaaa tgtacgattt ctgaaatcat accaattttt tcagctgctt
     1381 tcttttttga tctaacataa ctttgactag cgccatcatt accaactaat ataacggata
     1441 atttaggtgt aaaacccttt tcttttagcg cttcaacttg atcttgtaac ccctgtctgt
     1501 agtctttggc aatttgttta ccatctaaaa ttttagcaac cataaaaact tctcctccta
     1561 ttgtcaatat cctaactttg ttagtttata aaactaattt acatagcaaa agacgtaaat
     1621 tcaaacaaaa aatcaaaata aagttcgatt tttgattgaa aaagcagaaa ttgcttgtta
     1681 tgctatatct ataatataca actaaagtaa ttcacaattc aaacttttga aagggtgtac
     1741 aaagtgaaag tagcagtcat tatgggcagt tcttccgatt ggaaaattat gcaagagagt
     1801 tgtaacatgt tggattattt tgaaattccg tacgaaaaac aagtagtatc cgcacatcgt
     1861 acgccaaaaa tgatggttca atttgcttct gaagcgagag aaagaggtat aaacattatc
     1921 attgcaggcg ctggcggtgc ggcacattta ccaggtatgg ttgcatcatt aacgacgcta
     1981 ccagttattg gagtgccgat tgaaacaaaa agtttaaagg gtatagattc tttattatca
     2041 attgttcaaa tgccaggagg tattccggtt gcaacgactg caattggtgc agcaggtgct
     2101 aaaaacgcag gtatacttgc agcaagaatg ttaagtattc aaaatccttc tttagttgaa
     2161 aaactaaatc agtatgaatc ttcgttaatt caaaaagtgg aggacatgca aaatgaactt
     2221 caataaatta aagtttggtg cgactattgg cattattggt ggtggtcagc ttggaaagat
     2281 gatggcacaa tcagctcaaa aaatgggtta taaagtggtt gtattggatc cttctgaaga
     2341 ttgtccatgt agatacgttg cacacgaatt tatacaagcc aagtatgacg atgaaaaggc
     2401 actcaatcaa ttaggacaaa aatgtgatgt gattacttat gaatttgaaa acatttcagc
     2461 ccaacaatta aaactattat gtgaaaagta caatattccg caaggttacc aagctataca
     2521 gttattacaa gatcgcttaa ctgaaaaaga aacattaaaa agtgctggta ccaaagttgt
     2581 cccgttcatt tcagtaaaag aatctacaga tattgacaaa gcaattgaaa cattaggata
     2641 tccttttatt gtaaaaacta gatttggtgg ctacgatggc aaaggtcaag ttttaattaa
     2701 caacgaaaaa gacttacaag aaggttttaa attaattgaa actagtgaat gcgtagctga
     2761 aaaatatttg aatatcaaga aagaagtatc tcttactgtt acaagaggaa acaacaatca
     2821 aatcactttt ttcccattac aagaaaatga gcatagaaat caaatacttt tcaaaacaat
     2881 tgttccagcg agaatagata aaacagctga ggcgaaagag caagttaata aaattatcca
     2941 atcgattcat ttcattggaa catttacagt tgaatttttt atagatagta acaaccaatt
     3001 gtatgtgaac gagatagcac caaggcctca caattccgga cattattcaa ttgaagcatg
     3061 tgattattca caatttgata ctcatatttt agcagttacc ggacaatcat taccaaattc
     3121 aattgaatta ttaaagccag cagtcatgat gaacttacta ggtaaagatt tagatttatt
     3181 ggaaaatgaa tttaatgaac atccagagtg gcacttacat atttatggta agtctgagcg
     3241 taaagatagc agaaaaatgg ggcatatgac tgtactaacg aatgatgtaa accaaactga
     3301 acaagatatg tacgctaaat ttgaggggag taattaaagc atgacattat tatatgaagg
     3361 aaaagcgaag cgcattttct caacaaatca agaaaatgaa ttaagagttg aatataaaga
     3421 tgaagttact gctggaaacg gggctaagaa agacacaatg gcaggtaagg ggcgattaaa
     3481 taatcaaatt acttctatta tatttaaata tttacaagaa aatggaatag aaagtcactt
     3541 tattaaacaa ttatctgaaa cagaacaatt agttaagcct gtgaaaataa ttccattaga
     3601 agtagttgtt cgtaatattg ctagtggatc tattacaaag cgtttaggtt ttgaaaatgg
     3661 tgaagttttt agagaaccac ttgtagaatt tttctataaa aatgatgcgt taaatgatcc
     3721 gttgataacg gatgaccatg ttaaattgct caatatagca tcagatgaag atattgaaat
     3781 actaaaatcc aaagcattaa agattaataa tgtgttgaaa caattaatgg atgctatgaa
     3841 tttaaaatta gtagatttta aaatcgaatt tggaaagact gagactggtc aaattttgtt
     3901 agcggatgaa atatctccag atacatgtcg aatttgggat aaagctacca atgcaaactt
     3961 tgataaagat gtatatagaa ataacactgg atcactgatt gaaacatatc aaatattttt
     4021 aaacaaattg gaggatttaa aataatgaaa acaattgaac tacatatcac attacaacca
     4081 caagtattag atacgcaagg acaaacgctt actcgagctg tacatgactt aggttatgca
     4141 caagtgaatg atattcgtgt aggaaaagta ttatatatga cagtggatga ggttagtgat
     4201 gaaaaggtac acaacattat tacaactcta agtgaaaaat tgtttgcaaa tacagtgatt
     4261 gaagaatata gctataaagt gttagatgat gaaaaggaga atgcataaaa tgaaatttgc
     4321 ggttcttgtt tttccaggtt cgaattgtga tagagacatg tttaatgctg ctattaaaag
     4381 tggtgttgaa gcggaatatg tagattatag agaaacatca ctaagtggat ttgatggcgt
     4441 acttattcct ggtggatttt cattcgggga ttacttaaga tctggggcaa tggctagtgt
     4501 agcgccgatt atttcggaag ttaaacgtct tgcaactgaa ggtaagccag tattaggtgt
     4561 ttgtaatggg tttcaaattt taactgaaat aggcttatta cctggtgcat tattgcataa
     4621 cgattcacat ttatttatta gtagaaatga agagttagaa atagtgaata atcaaacggc
     4681 atttacaaat ctttatgaac aaggtgaaaa agttatatat cctgtagctc acggtgaagg
     4741 tcattattat tgtactgatg aaatatatca acaattaaaa gctaacaatc aaattattct
     4801 gaaatatgtg aataatccga acggttcata tgatgatatt gcaggaattg ttaacgaaaa
     4861 aggcaatgta tgtggcatga tgccacatcc tgaaagagct ttagaaacgt tgttaggtac
     4921 tgatagtggt gtgaaattat ttgaagcgat ggtaaaaagt tggagggaac aacatgtcta
     4981 aatttatcga accaagcgtt gaagaaatta aacttgaaaa agtatatcaa gatatgggat
     5041 taagtgatca agaatatgaa aaagtttgcg atattttagg cagacaacct aactttacag
     5101 aaacaggtat cttttctgtt atgtggagtg aacattgctc ttataaacat tctaaaccgt
     5161 ttttaaagca atttcctacg tcaggtgacc atgtgcttat ggggcctggt gaaggtgcag
     5221 gggtagtcga tataggtgat aatcaagccg tagtatttaa agtagagtct cacaatcatc
     5281 catcagcaat tgaaccatat caaggggctg ctacaggcgt tggtggaatc attcgtgaca
     5341 ttgtctctat tggggctaga cctattaatt tgttaaacag tcttagattt ggagaattag
     5401 ataataaaca aaaccaaaga ttacttaaag gtgttgtaaa gggtatcgga ggttatggta
     5461 actgcattgg tattccaaca actgctggtg aaatcgaatt tgatgaacgt tatgatggca
     5521 atccacttgt taatgcaatg tgtgttggtg ttatcaatca cgacatgatt caaaaaggca
     5581 cagcaaaagg tgtaggtaat tcggtcattt atgttggttt gaaaactggt cgagatggta
     5641 ttcatggtgc tacttttgca tctgaagaat tgacggaaga aagcgaaagt aaacgacctt
     5701 ctgtacaaat cggtgatcca tttgtaggta aaaaattaat ggaagcaaca cttgaagcaa
     5761 ttacatttga tgaattagtt ggtattcaag atatgggtgc tgctggttta acatcttcat
     5821 cgtctgaaat ggcggcaaaa ggtggtagtg ggttacattt gagattagaa caagtgccaa
     5881 cacgtgagcc aggtatttct ccttatgaaa tgatgctttc agaaactcaa gaacgtatgt
     5941 tactagttgt tgaaaaaggt actgaacaaa aattcttaga tttatttgat aagcacgaat
     6001 tggatagtgc tgttataggt gaagttacag atacaaatcg ttttgtttta acatatgatg
     6061 acgaagttta tgctgacatt ccagttgaac cactagctga tgaagcacct gtatatattt
     6121 tagaaggaga agaaaaagat tataatactt ctaaaaatga ttatacacac atcgatgtta
     6181 aagatacttt ctttaaatta cttaagcatc cgactatagc atctaaacac tatttatatg
     6241 atcaatacga ccaacaagtt ggtgccaata cgataattaa gccaggactt caagcatcgg
     6301 tagtacgtgt ggaaggcaca aataaggcaa ttgcttcaac aattgatggt gaagcgcgtt
     6361 atgtatataa caatccatat gaaggtggaa agatggtagt agctgaagct tatcgaaatt
     6421 taattgccgt gggtgcaaca ccattagcaa tgacagattg tttaaattat ggttctcctg
     6481 aaaagaaaga aatctatcaa cagttgatag attcaacgaa aggtatggca gaagcatgcg
     6541 acattcttaa gacaccagta gtttctggta atgtatcttt atataacgaa acgaaaggta
     6601 cttctatttt cccaacacca gttgttggaa tggtaggttt gattgaaaat gtaaattatt
     6661 taaatgattt tgaacctcaa gttggagata aattatattt aatcggtgat actaaggacg
     6721 actttggtgg tagtcaactt gaaaagttaa tttatggcaa agttaatcat gaatttgagt
     6781 cattagattt gagttcagaa gttgaaaaag gtgaatcaat caagaccgct attcgtgaag
     6841 gactattatc acatgttcaa acagttggta aaggtggctt actgattacc ttagctaaac
     6901 taagtgcgca ttacggttta ggattaaaat cttcaataga tataacaaat gcacaattgt
     6961 ttagtgagac gcaaggccga tatgttgttt ctgttaaatc aggtaaaact ttaaatattg
     7021 ataatgcaat agaaattgga cttttaacag atagtgataa tttcaaggta acaacaccat
     7081 atacagagat tagtgaaaat gtttcagata ttaaacaaat atgggaaggg gcaattgctc
     7141 aatgtttaac tactcaggat taaacgaaga atgtggcgtg tttggtattt ggaatcatcc
     7201 tgaagcagcg caactaacat atatgggact tcatagtttg caacatcgtg gtcaagaagg
     7261 tgcaggtata gttgtttctg atcaaaatga attaaaaggc gagcgaggat taggcttact
     7321 aactgaagcg attaaagatg atcaaatgga acgattaaaa ggatatcaac atgcaattgg
     7381 tcacgtccgt tatgctactt caggtaataa aggtattgaa aatattcaac cgtttctgta
     7441 tcacttttat gatatgagtg taggtatttg tcataatggt aacctcatta atgctaaatc
     7501 attgcgtcag aatttagaaa aacaaggtgc tatcttccat tcgtcttctg atactgaagt
     7561 cattatgcat ttgatacgtc gaagtaaagc tcctactttt gaggaagcgt tgaaagaaag
     7621 tttgcgaaaa gttaaaggcg gttttacatt tgcgatttta actaaagatg cgttatatgg
     7681 cgcagtagat ccaaatgcta tcagaccact tgttgtaggt aaaatgaaag atgggacata
     7741 catccttgca agtgaaacat gtgcaataga tgtgttaggt gcagaatttg ttcaagatat
     7801 tcatgcaggt gaatatgtcg tgattaacga taaaggtatt acagttaaat cttatacaca
     7861 tcatacgaca actgcaattt ctgcgatgga atatatttat tttgctagac cagactcaac
     7921 aatagctggt aaaaatgtcc atgcagtacg taaagcttct ggtaaaaaat tagcccaaga
     7981 aagccctgta aatgctgata tggtcatcgg tgtacccaat tcatcgctat cagctgcgag
     8041 tggttatgct gaagaaatag gtttgccata tgaaatggga ctagttaaaa atcaatatgt
     8101 tgcaagaaca tttattcaac caactcaaga attacgtgag caaggtgtga gagtgaagtt
     8161 atctgcggta aaagatatag tagatgggaa aaatatcatt cttgttgatg attccattgt
     8221 tcgcggtacg acaattcgac gcattgtgaa aatgttaaaa gattctggtg caaataaagt
     8281 gcatgtgcgt atagcatcac cggaatttat gtttccaagt ttttacggaa tcgatgtttc
     8341 aactacggca gaattaattt ctgcaagcaa atcacctgaa gaaattaaag attatattgg
     8401 cgctgattca ttagcatatc tatctgtaga tgggttaatt gaatcaattg gtttagatta
     8461 tgacgcgcca tatagtggct tatgtgtaga aagtttcact ggagattatc ctgcagggtt
     8521 atatgattat gaagcaaatt ataaagcgca tttaagtcat cgacaaaagc aatatatttc
     8581 taaaaacaaa cacttttttg atagcgaggg aaatttaaat gtctaaagca tatgaacaat
     8641 ctggtgtaaa tattcatgct ggttatgaag ctgtagaaag aatgtctagt catgttaaac
     8701 gtacgatgcg taaagaagtt atcggtggtt taggtggatt cggtgctaca tttgatttat
     8761 cacaattaaa tatgacagcg ccagttttag tttctggaac agacggagta ggtacgaaat
     8821 taaaactagc tatcgactat ggtaaacatg attcgatagg tatcgatgca gtcgcaatgt
     8881 gtgttaatga tattttaacg acaggtgcag aaccattata ttttttagat tacattgcta
     8941 caaacaaagt tgttcctgaa gttattgaac aaattgttaa aggtattagt gatgcatgtg
     9001 ttgaaacgaa tactgcactt atcggtggag agactgctga aatgggtgaa atgtatcacg
     9061 aaggcgaata tgatgtagct ggatttgctg ttggagcagt tgaaaaggat gactatgtag
     9121 atggttcaga agtgaaagag ggacaagttg ttatagggct tgcgtcaagt ggcattcatt
     9181 caaatggata cagtttagtg cgcaaattaa ttaatgaatc aggcattgat ttggcatcaa
     9241 acttcgataa tcgtccattt atcgatgtct ttttagaacc aactaaatta tatgtcaaac
     9301 ctgtacttgc tttgaaaaaa gaagtttcta ttaaggcaat gaatcatatt actggtggag
     9361 gtttttatga aaatattcca cgtgcattgc cagccggata tgctgctaga attgatacta
     9421 catcatttcc aacaccaaaa atatttgatt ggttacaaca acaaggcaat atagacacaa
     9481 atgaaatgta taacattttt aatatgggta ttggctatac ggttatcgtt gatgaaaaag
     9541 atgtatcacg cgctttgaag attttagcag aacaaaatgt ggaagcctat caaattggtc
     9601 atattgtgaa aaatgagtca actgcaattg aattgttggg ggtataacaa tggttaaaat
     9661 tgcgattttt gcatcaggtt caggaagtaa ctttgaaaat atagttgagc atgttgaatc
     9721 aggaaaactt gaaaatattg aagttacggc gctatatacg gatcatcaaa atgcgttttg
     9781 tatagataga gcaaaaaagc acgatattcc tgtttatatt aatgaaccaa aacaatttga
     9841 ttcaaaagca gcgtatgaac aacatttagt aacattatta aatgaagata aggtagagtg
     9901 gattattcta gctggctaca tgcgtctaat tggtccagac ttattagctt catttgaagg
     9961 taaaatattg aatatacatc catctctatt gccgaaatat aaggggattg acgcaatagg
    10021 ccaagcatat catagtggcg atactattac tggttcgaca gtacattatg ttgatagtgg
    10081 tatggatacg ggagaaatta ttgaacagag aaaatgtgat attagaccgg acgattcaaa
    10141 agaacaatta gaagagaaag taaaaaaatt ggaatatgag ttatatccaa gtgttattgc
    10201 taaaattgta aaataaggag tatgtatgtg atgaagaaag ctattttgag cgtatcaaat
    10261 aaaacaggta ttgtagagtt tgctaaagcg ttaacgcaat taaattatga attatattca
    10321 acaggtggta ctaaacgtat attagatgaa gcaaatgtac cagttcgttc tgtttcagac
    10381 ttaacacatt ttccagaaat aatggatggc cgtgttaaaa cattacatcc ggctgttcat
    10441 ggtggcattt tagctgatcg aaataaaccg cagcatttaa atgaattatc agaacaacat
    10501 atagatttaa ttgatatggt agtagttaat ttatatccat tccaacaaac tgttgcaaac
    10561 cctgatgtga cgatggacga agcaattgaa aatattgata ttggtggtcc aacaatgtta
    10621 cgtgctgcag ctaaaaacta taaacatgta acaacaattg tacatccggc agattatcaa
    10681 gaagtattga cgcgattaag aaacgattcg ttagatgagt catatagaca atcattaatg
    10741 attaaagttt ttgagcatac tgcagaatat gatgaagcga ttgtacgttt ctttaaaggg
    10801 gataaagaaa ctttaagata tggagaaaat ccacaacaat cagcgtattt tgtgagaact
    10861 tcgaatgcta agcacacgat tgcaggcgct aaacaattac atgggaaaca attaagctat
    10921 aacaatatta aagatgcaga tgctacacta gctttagtta aaaagtttga tacacctgct
    10981 acagttgcgg ttaaacacat gaatccatgt ggtgttggta tcggtgacac gatagaacaa
    11041 gcatttcaac atgcgtatga agcggatagt caatcaatat ttggtggaat tgttgcatta
    11101 aaccgagctg taacacctga gttagcagag caattgcata gtatcttttt ggaagtcatt
    11161 attgcaccaa aatttacaga tgaagcatta gatattttaa aacaaaagaa gaatgtaaga
    11221 ttattagaaa ttgatatgac tatagacagt aacgaagaag agtttgtttc agtatctggc
    11281 ggatatttag ttcaagataa agacaattat gtcgtgccaa aagaagaaat gaaagttgtt
    11341 acagaagtag cacctacaga tgaacaatgg gaagcaatgt tattaggatg gaaagttgta
    11401 ccatcagtaa aaagtaatgc aattatttta agtaataata aacaaactgt aggtataggt
    11461 gctggacaaa tgaatcgtgt cggtgctgct aaaattgcgt tagagagagc tattgaaatc
    11521 aatgatcatg tagcgttagt atctgatgga tttttcccta tgggagatac agttgaactt
    11581 gcagcacaac atggtataaa ggcaattatc caaccgagtg gttcgattaa agatcaagat
    11641 tcaattgata tggctaataa acatggtatt gcaatggtag tcacaggcac tcgacatttt
    11701 aaacactaat tttaaagggg cgtaacataa atgaatgtat tagtaattgg tgctggtgga
    11761 cgagaacatg cacttgcata taaacttaat caatcgaatc tagttaaaca agtgtttgtc
    11821 attccaggta atgaggcaat gacacctata gctgaagtac acactgaaat ttcagaatct
    11881 gatcatcaag cgatactaga ttttgctaaa cggcaaaatg ttgattgggt agttataggt
    11941 ccagaacagc cgctaattga tggattagca gacattttac gagcgaatgg tttcaaagtg
    12001 tttggtccaa ataagcaagc agctcaaatc gaaggctcaa aattatttgc taaaaagata
    12061 atggaaaaat ataatattcc aactgctgat tataaagaag ttgagcgaaa aaaggatgct
    12121 ttaacatata ttgaaaactg tgaattgccc gttgttgtca agaaagatgg gttagctgct
    12181 gggaaaggcg ttattattgc agatactatt gaagcagcca gaagtgctat tgagattatg
    12241 tatggtgatg aagaagaagg tactgttgta tttgaaacgt ttttagaagg tgaagagttc
    12301 tcgctaatga catttgttaa tggtgattta gcagtacctt tcgactgtat tgcacaagat
    12361 cataaacgcg catttgatca tgatgaagga ccaaatactg gtggtatggg ggcttattgt
    12421 ccagtaccac atattagtga cgatgtttta aaacttacaa atgaaacaat tgcacaaccc
    12481 attgcaaagg caatgcttaa tgaaggttat caattcttcg gtgtattata cattggtgct
    12541 attttaacta aagatggtcc aaaagtaata gaatttaatg cccgttttgg tgatcctgaa
    12601 gctcaagtat tattaagtcg catggaaagt gatttaatgc agcatattat tgatttagat
    12661 gaaggaaaac gtactgaatt caaatggaaa aatgaatcta ttgtaggggt catgttggca
    12721 tcaaaaggat atcctgatgc atatgaaaaa gggcataaag taagtggctt tgatttaaat
    12781 gaaaactatt ttgttagtgg attaaagaag caaggtgata cctttgttac ttcaggtggt
    12841 agagttatac ttgccatcgg aaaaggtgac aatgtacaag atgcacagcg agacgcatac
    12901 aaaaaagtat cacaaataca aagtgaccat ttattctatc gtcatgacat tgcgaataaa
    12961 gcactacaac ttaaataagt aaatttaaaa tactaagatt agctatgaac gaatctataa
    13021 cgatagattt tttcatagct tttttagttg tagagtctag gacattgatt tctgtaccaa
    13081 atttgtgatt atgcatatgt aatacaaaag aggcgccaca acatgtttgg atgaacaaaa
    13141 taacatgttt gtggcacctc ttttgtttag tatggaataa atggttttct ttttctatac
    13201 aatgaatttc taatttagta tctatacaat tatggataaa atttaaccta cacgaccaag
    13261 acgaacatca tctatgcccg tgatgggtaa ggtgattgaa caataatatg ccatagtaat
    13321 aatggcaatt aaaactataa taaagattat atctttatat gagaaaggta cgttgtaata
    13381 gtaagtacga ggaccatctc taaatccttt cgactccatc gcaactgata attgatgtgc
    13441 ctttctaata ttttggctta atagaggtat aattaaatgc ttaaatcgct ttaaccctct
    13501 ataatttgcc gcgtctatca tctgatagcg catttttaaa gatctgcgaa gctgtattaa
    13561 agaactaatc attaaaggta tcatacgaat ggcagccatg aatgcataag caacttttga
    13621 tttaaccttt aaatgttgca ttaaactata aaatatcatg acaacctgag atgtaagtgc
    13681 gattaagata ccgaaaaatg aaatagcaat ggttcttaat gatacatgta aaccacgaac
    13741 taaactttct gttgtaatat ggataaatcc gaatttcaaa attgtatggc taccattccc
    13801 gtataaaatc atgaacaggg aagagagtaa tgcaaagcca atacttatag ttataaaaat
    13861 tgctgtaatt ttaaactgag taccattaaa catcaataag aaaactaaca ttaagatagt
    13921 gatataaagc ataaaatcga aattatgcac aaatataata aagaaaaata gtataattcc
    13981 aagaaatagt ttcgttataa tgttgacatc atcaacaaat gattgccgaa ctttccattg
    14041 ctcatacatt cgtatcacca tcacaatcta gtaacgcacc atctgaaatt ttaagtcttc
    14101 ttgatggata acgttcaatt atttcatcgt catgtgtaac catgacaata ctttgtccca
    14161 aattaattcg cttttggaaa agtttgatca actggaatgt attatggcta tcaagtccaa
    14221 atgtcggttc atctaaaaag ataatatcag ctttagaact tagtgcggta gctacgctaa
    14281 ggcgtcgttt ttgaccaata gacaactcat aaggatgttg atcttttaca ttttgtaaat
    14341 ctaaaagttt taaaagttgt atcgtttcat catcactttg atctttagaa aggtgattaa
    14401 aatgaatgtt aatttcatca taaaccgaat ttgttataaa ttgtaattct gggttttgat
    14461 aaactaggta catgtgtttt gctgcatgtt taatttttgt taaacgctga ttttcaaaat
    14521 aaacatcacc ttgatattta atcaattgca taattgattc aagcaaggtt gttttaccac
    14581 taccatttgc ccctgtaatt gtaatccact cacctagacc aatttctaaa tctgagaatg
    14641 agagcaatgt tgatttaccg cgaataatac gtccattttt aaattgtaat aagtgtgagt
    14701 ttgttgttgg aaagtcaaca cgacttggtg cgaattccca tgcacgtgga tgccacacac
    14761 catattcact gagtaaatga acatacttct gtaatatgat ttcaggacat tcatcggcaa
    14821 tgatatttcc gttataatcc atcaaaatga cgcggtcgac atgattccag atgtgtttaa
    14881 ctttatgttc aacgattaca accgtttgat cttcccaaag ttcaattagt ttagtccata
    14941 aatcttctgt tgcttgaaca tctaacattg ctgtcggttc atctaaaaac aatgtttttg
    15001 attgttgaag aatggtttca acaattgcca atttctgttt catcccgcca cttaaatctt
    15061 tgatatacgt ttcaggggta acatttaaat tgaccatatt taaagcattg ataattaacg
    15121 catccatgtc ttcacgtggt aattgtctat tttctaaaac gaatgcaagt tcttcgtata
    15181 cttttggcat acaaaactgg ctatcagggt cttggaaaat aacgccactt aatgggtcaa
    15241 cgattagttc atcatatttc ataggtaatt caattaaatt aggaacaata ccacttaata
    15301 cattcagaag tgtactttta ccgcaaccag aaggaccgag taaaagtact ttttctttgt
    15361 cttgaatagt gatatttaaa tgatcgaaaa ttttacgttg accacttgga tattttaatc
    15421 gtaaatcact tacttttaac actctgaatg ctcctcttat aagttgtcgt aatcttcttt
    15481 agcagctggt ctaaataatt ttgttacgcc tgtcttatct aaagctttta ctaaaaggta
    15541 agataggacg ccggcgacta ctgcaccact aattaatcta aatacgatga ataatgttaa
    15601 gttccaacct gcaacttcat ttaaataacc atagaaataa tctatcggga aagccgcgat
    15661 tgctgtacaa aaacctgcta acatagctac cataactgaa cgtgattgat atttaaaaat
    15721 tgcaaagaca agttcacacg ctaaaccttg tataaaagcg taaacgattg tcggaatatc
    15781 gaaacgaccc ataatgatag tttcgccggc acctgcagca aattcagcca gtaaagcaat
    15841 acctggtttt ggaataatta gatagcagac aatcgctgcc atgaaccaaa ccccgtttgt
    15901 taattgttcg aggtgaaggc ctgtagcttg cacaccattg taaacaaacc accataaatt
    15961 gtaaataact gcgaatacta ctgaaataag tacggttact agtatttcag atagctttaa
    16021 accttttgac atttttacat cctcctaata aaaaaacgca caaccatcca taggaaagtt
    16081 atgcgttcac aatatatatt agtaaaacat atgtatagta acactttcct acgctagttc
    16141 aagctagatc aggttcaaag ggtttgaggg caagcctcat ctcagtataa aacaccccta
    16201 gtgtgtgcga tttatttaat taattatact gtaagacgtt tgtaaactta tgtcaatagg
    16261 ttgtcttcat gaaatttcgt ttaattcgat ttaaaattta taatattagc attggattta
    16321 aattgaagat gtagtaggaa tgttagtaat taaagatata aaaatatgtg acatgtaata
    16381 atattgagct gataaatgaa gagggatact tatcaatcat acctctttaa caacagtgaa
    16441 gaacccgtgc ataatggctt acgaattata gtttataagg aagaagaggg atacatgcgc
    16501 cgagcacatg cataaaagcc cctaacaact aaaagttgta aggaaggaga gggatacatg
    16561 cgccgagcac atgcataaaa tcccctaaca actaaaagtt gtaaggggat ttaaattaat
    16621 ttagtgtatc ttggatatct tgttttgttt gattaatatc ttctgttttt tcttcttttt
    16681 tatcttttaa tttttcttca acttctttag ctttttctgc tgctttttta ttttgatttt
    16741 cattagacat gattaattcc tcccaaattg gataattatt tatatataaa tcttacccgg
    16801 ttgtactttc gttaaacttt tctaagtcta tagcactatt tattcattta tctaaagaca
    16861 acaacattag attaatatat aatgattttg aggtgaacat aatgtctttt cttaggaaac
    16921 acgccgaaat tatttttagc tatttaatcg gtatcgtttc actcttcact ggtctcatta
    16981 ttttaattaa cttgccatta attaaacaat taaatggtgg taaaaaagtt gatacacatg
    17041 ttcataatgt gtgggaattt ctgaatgcat ttttcagtga aattattaaa gtaatgagtc
    17101 gatttatagg taatttccct atagttagtg caattgtgat aattatattc ggtattttag
    17161 ttatgttgat tggtcataca ttacttagaa ctattaagta tgact
//
LOCUS       DAMQYT010000033        16792 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMQYT010000033 DAMQYT010000000
VERSION     DAMQYT010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16792
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.033"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            429..1469
                     /locus_tag="PGA33_000893"
     CDS             429..1469
                     /locus_tag="PGA33_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDG6472722.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKENQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
     gene            1483..2550
                     /locus_tag="PGA33_000894"
     CDS             1483..2550
                     /locus_tag="PGA33_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDG6472723.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPSEQIISRLKAANVKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVITKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELIHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            2617..2715
                     /locus_tag="PGA33_000895"
     CDS             2617..2715
                     /locus_tag="PGA33_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472724.1"
                     /translation="MRFYFTNLIKRKHQAEDRRKILIKQKHQTKVR"
     gene            2763..3611
                     /locus_tag="PGA33_000896"
     CDS             2763..3611
                     /locus_tag="PGA33_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDG6472725.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            3624..4451
                     /locus_tag="PGA33_000897"
     CDS             3624..4451
                     /locus_tag="PGA33_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDG6472726.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            4478..5797
                     /locus_tag="PGA33_000898"
     CDS             4478..5797
                     /locus_tag="PGA33_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDG6472727.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            5881..6798
                     /gene="lipA"
                     /locus_tag="PGA33_000899"
     CDS             5881..6798
                     /gene="lipA"
                     /locus_tag="PGA33_000899"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDG6472728.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            6928..7311
                     /locus_tag="PGA33_000900"
     CDS             6928..7311
                     /locus_tag="PGA33_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDG6472729.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(7390..7656)
                     /locus_tag="PGA33_000901"
     CDS             complement(7390..7656)
                     /locus_tag="PGA33_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HDG6472730.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            7754..8188
                     /locus_tag="PGA33_000902"
     CDS             7754..8188
                     /locus_tag="PGA33_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HDG6472731.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVGIRKQFTYDYTALD
                     IKIIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            8188..8967
                     /locus_tag="PGA33_000903"
     CDS             8188..8967
                     /locus_tag="PGA33_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDG6472732.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            9000..9959
                     /locus_tag="PGA33_000904"
     CDS             9000..9959
                     /locus_tag="PGA33_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HDG6472733.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
     gene            10213..10323
                     /locus_tag="PGA33_000905"
     CDS             10213..10323
                     /locus_tag="PGA33_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472734.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            10371..10523
                     /locus_tag="PGA33_000906"
     CDS             10371..10523
                     /locus_tag="PGA33_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDG6472735.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            10539..11996
                     /gene="dltA"
                     /locus_tag="PGA33_000907"
     CDS             10539..11996
                     /gene="dltA"
                     /locus_tag="PGA33_000907"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDG6472736.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVNR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            11993..13207
                     /gene="dltB"
                     /locus_tag="PGA33_000908"
     CDS             11993..13207
                     /gene="dltB"
                     /locus_tag="PGA33_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HDG6472737.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            13225..13461
                     /gene="dltC"
                     /locus_tag="PGA33_000909"
     CDS             13225..13461
                     /gene="dltC"
                     /locus_tag="PGA33_000909"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HDG6472738.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            13458..14633
                     /gene="dltD"
                     /locus_tag="PGA33_000910"
     CDS             13458..14633
                     /gene="dltD"
                     /locus_tag="PGA33_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDG6472739.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKEYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(14896..15138)
                     /locus_tag="PGA33_000911"
     CDS             complement(14896..15138)
                     /locus_tag="PGA33_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDG6472740.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            15239..15562
                     /locus_tag="PGA33_000912"
     CDS             15239..15562
                     /locus_tag="PGA33_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HDG6472741.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            complement(15621..16685)
                     /locus_tag="PGA33_000913"
     CDS             complement(15621..16685)
                     /locus_tag="PGA33_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDG6472742.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
BASE COUNT         5998 a         2476 c         3018 g         5300 t
ORIGIN      
        1 gaattgaata acctaaactt acactggcac tgattattgt aaatattgct aaaacaaaat
       61 acatgttaat ccttctttct atatttggat ataaacaagt acttgtctaa agttatttaa
      121 aagataatta gaataaattt atgagaaact ggttgttatc attataatgg tttcaaatga
      181 ttataactat gtcataaact gaatttgttg aaatttttca ttatgcaaat ttattaataa
      241 caaacagctc gaactatagc atcattttac taatgaatgc attaaagtaa ctatgactaa
      301 aaatgcatat taattatcat tattaagact attatatata atgaatttta actggtttat
      361 taaacgagaa cgtcgggaat taagtaacta caataaaaat aagatatgac aataaggaga
      421 ctacacgcgt gatcattgcc ataattatat tgatatttat ttcgtttttc ttttcaggaa
      481 gcgagacggc attaacggct gccaataaaa caaaatttaa aactgaagct gacaaaggtg
      541 ataaaaaagc aaaaggcatt gtaaagttac ttgaaaaacc aagtgagttt attacaacga
      601 ttctaattgg gaataatgtc gcgaatattt tattaccaac acttgttaca attatggctt
      661 tacgttgggg gattagcgtt ggtattgcat cagctgtttt aacagttgtt atcattttga
      721 tctccgaagt gattcccaag tctgtcgctg caacatttcc agataaaata acaaggcttg
      781 tatatccaat tattaatatt tgtgtcattg tgttccgtcc tatcacatta cttttaaata
      841 agttgacgga cagtattaat cgaagtttat ctaagggcca acctcaagaa catcaatttt
      901 caaaagaaga atttaaaaca atgttagcaa ttgctggaca tgaaggtgct ttaaatgaaa
      961 ttgagacgag taggttggaa ggtgtcatta attttgaaaa tttaaaagta aaagatgtag
     1021 atacaacacc tagaattaat gtgacggcat ttgcttcaaa tgcgacatac gaagaagttt
     1081 atgaaacggt tatgaataag ccatacacta gatatccagt gtacgaggga gatattgata
     1141 acattattgg ggtgtttcat tctaaatatc tgttggcttg gagtaataaa aaagaaaatc
     1201 aaattacaaa ctattcagct aagccattat ttgtgaatga acacaataaa gctgaatggg
     1261 tattacgtaa gatgactatt tctagaaaac atttagcaat tgtgttggac gaatttggtg
     1321 gtactgaagc gatagtgtca catgaagact taattgaaga attattaggt atggaaattg
     1381 aagatgagat ggataaaaag gaaaaagaaa aactttctca acagcaaatt caatttcaac
     1441 aacggaaaaa tcgcaacgta tctatataag gagcgaacag ctatgtggaa taagaatcga
     1501 cttactcaaa tgttaagtat tgaatatcca attatacaag caggtatggc aggaagtacg
     1561 acaccgaaat tagttgcatc agtaagtaac agtggtgggt taggcacaat aggcgcaggt
     1621 tactttaata cgcagcaatt ggaagatgaa atagattatg tacgccaatt aacgtcaaat
     1681 tcttttggcg taaatgtctt tgtaccaagt caacaatcat ataccagtag tcaaattgaa
     1741 aatatgaatg catggttaaa accttatcga cgcgcattac atttagaaga gccggttgta
     1801 aaaattaccg aagaacaaca atttaagtgt catattgata cgataattaa aaagcaagtg
     1861 cctgtatgtt gttttacttt tggaattcca agcgaacaga ttataagcag gttgaaagca
     1921 gcgaatgtca aacttatagg tacagcaaca agtgttgatg aagctattgc gaatgaaaaa
     1981 gcgggtatgg atgctatcgt tgctcaaggt agtgaagcag gtggacatcg tggttcattt
     2041 ttaaaaccta aaaatcaatt acctatggtt ggaacaatat ctttagtgcc acaaattgta
     2101 gatgtcgttt caattccggt cattgccgct ggtggaatta tggatggtag aggagttttg
     2161 gcaagtattg tcttaggtgc agaaggggta caaatgggca ccgcattttt aacatcacaa
     2221 gacagtaatg catcagaact actgcgagat gcaattataa atagtaaaga aacagataca
     2281 gtcattacaa aagcgtttag tggaaagctt gcacgcggta tcaacaatag gtttatcgaa
     2341 gaaatgtccc aatacgaagg cgatatccca gattatccaa tacaaaatga gctaacaagt
     2401 agcataagaa aagccgcagc aaacatcggc gacaaagagt taatacatat gtggagtgga
     2461 caaagcccgc gactagcaac aacgcatccc gccaacacca tcatgtccaa tataatcaat
     2521 caaattaatc aaatcatgca atataaataa tcgaccgcaa tccacaaaag cacaagcacc
     2581 cccaaacatt attttagtgc ttgccatttt tgtggattgc gtttctattt taccaattta
     2641 atcaaacgaa aacatcaagc tgaagatcgc cgaaagattt taatcaagca aaaacatcaa
     2701 actaaagttc gctgaaatga ttatgataaa agttatatgg tatgatgaca ttggtgatat
     2761 atatgataaa catcggatta acaggttggg gtgatcacta ttcattatat gaagatttag
     2821 aacgccaaac cgataaactt aaaacatatg ctggacattt tccggttgtc gaattagatg
     2881 cgacatacta tgcgatacaa ccggaaagaa atatattgaa atggataaaa gaaacgcctg
     2941 atacatttga atttgtggtc aaaattcatc aagcactcac attgcatgca gactacaaaa
     3001 catttgcaga tacaaggcaa gaactatttg atcaatttaa gaatatgtta gagcccttac
     3061 atacacagaa aaaattagca atggtattgg ttcaatttcc gccatggttt gactgcaatg
     3121 cacaaaatat caaatatatt ttgtatgtaa gacagcaatt acaagcattt ccaatgtgtg
     3181 tagaatttag gcatcaatca tggtttagtg atgcatttaa agaacaaaca ttggcatttt
     3241 taacagaaca tcaaatcatt catgcagtag ttgatgaacc acaagtgaaa gatggcagtg
     3301 tacctttagt caatcgaatc acaaatgaaa ttgcgtttgt acgttatcat ggacgtaatc
     3361 attacggttg gactaagaaa gatatgtcag atcaagaatg gcgcgatgta cgctatttat
     3421 atgattataa tgagcaagaa ttaatagact tggcacaaaa ggcacaaata ttagcacaaa
     3481 aagctaagaa agtttacgtc atatttaaca ataattctgg tggtcatgca gcaaataatg
     3541 ccaaaacata tcagcgatta ttgaatatag aatatgaagg gttagcacca caacaattaa
     3601 aattatttta agaggcgacg actatgttat taacaattac attattagtt ttaatcggag
     3661 gtttgtcagc gattataggg tctatcgtag gcattggagg cggtattatt atcgttccaa
     3721 caatggttta cctcggtgtt gaacatggat tactacataa tattacaaca caagtagcga
     3781 tagggacgtc ttcagtcatt ctaattgtga caggactttc ttcatcactt ggatatttaa
     3841 aaacaaaaca agttgatatt aaaaatggtt ccatcttttt atttggacta ttaccaggtt
     3901 cattgcttgg gtccttcatt agtagatatt taacatttga gtcatttaat ttatattttg
     3961 gtatcttttt aattttcgta gccattttat taatggtaag aaataagatt aaaccgttta
     4021 aaattttcga taaacccaag tatgaaaaga cttatgtaga cgctaaaggt aaaacatatc
     4081 attatagtgt tccaccattg tttgctttta ttacaacgtt tttaattggt atattgacag
     4141 gtttatttgg tattggaggt ggcgcactaa tgacgccact aatgcttatt gtatttagat
     4201 ttccacctca tgtagctgtt ggaacaagta tgatgatgat tttcttttca agtgtcatga
     4261 gttctatagg gcacattgct caaggtcacg tagcttgggg ttatgcaatc attttaatta
     4321 tttctagtta ttttggtgcg aaaatcggtg tcaaagtgaa tcaatcaatt aagtcagata
     4381 cggtagtaac attattgaga acagtaatgt tgttaatggg tatatattta attattcgtg
     4441 cgttgattta atacaacttt aaaaggagga cgtcaatttg aggcttacaa tttatcatac
     4501 gaacgatatt catagtcatt tacatgaata cgaacgcatt aaagcatata tggcagaaca
     4561 tcggccacga cttaatcatc cttctttata tgttgatcta ggtgatcatg tagatttatc
     4621 cgcacctata actgaagcaa ctttaggtaa aaagaatgtg gcattactaa atgaagcaaa
     4681 atgtgatgtt gcaacaatcg gtaataatga agggatgacc atttcacacg aagctttaaa
     4741 tcacctttac gacgaagcaa aatttatagt gacatgtagc aatgttatag atgaatcagg
     4801 tcatttacca aataatatcg tttcttctta tattaaggac atagacggtg tgaaaatact
     4861 attcgttgca gcgacagcac cttttacccc attttatcgt gcactaaatt ggattgttac
     4921 cgatccactt gaatctataa aagaagaaat tgaacttcaa cgaggtaaat ttgatgtatt
     4981 aatcgtgcta agtcattgtg gcattttctt cgatgaaaca ttatgccaag aattgcctga
     5041 aattgatgtc atttttggta gtcatacgca tcattatttt gaacatggtg aaatcaataa
     5101 tggtgtactg atggcggcag ctggaaagta tggtaattat cttggagagg ttaatttaac
     5161 ttttgaggca cataaagtag tacataaaac tgcaaagatt attcctttag aaacattacc
     5221 tgaagttgaa acttcatttg aagaagaagg aaaaacgtta atgtccaatt cagtaattca
     5281 acatccagta gtgcttaagc gtagtatgaa tcacataact gaagctgcat acttattagc
     5341 tcaaagtgtt tgtgagtata cacatgcaca atgtgccatc atcaatgctg gcttactcgt
     5401 taaagatatt gtaaaagatg aagtgacaga atatgacatt catcaaatgt taccgcatcc
     5461 gattaatatg gtaagggtta gactttttgg tgtgaaatta aaagagatta tagctaaaag
     5521 taataaacaa gaatatatgt atgaacatgc acaaggtttg ggtttcagag ggaatatatt
     5581 tggaggatat attctttata atttagggta cattcattct acagggcgtt actatctgaa
     5641 tggagaagaa atcgaagacg acaaagaata tgtactaggt acgatagata tgtatacgtt
     5701 cggtcgttat ttcccaacat tgaaagaatt accaaaagag tatttaatgc cagagttttt
     5761 aagagatata tttaaagaaa aattattgga atattaaaaa gtaagattat tggattttca
     5821 tttgtcatga atttcgatat aatgtttaaa gatacactta acaggagggt atgtgttgtt
     5881 atggcgacaa aaaacgagga aatattacgt aaaccggatt ggttgaaaat aaaattaaat
     5941 accaacgaaa actatacagg acttaagaag atgatgaggg aaaaaaatct taatactgta
     6001 tgtgaagaag ctaaatgtcc taatatacat gaatgttggg gtgcacgtcg tacagcgaca
     6061 tttatgattt taggtgccgt atgtacaaga gcttgtcgtt tttgtgcggt taagacaggt
     6121 ttacctaatg aacttgattt aaatgagcct gaacgtgtag ctgaatcagt tgaattaatg
     6181 aatttgaaac acgttgttat cactgctgtt gcgcgtgatg atttaagaga tgctggttca
     6241 aatgtttatg ctgagacagt acgtaaagtt agagaaagaa atccatttac aacgattgaa
     6301 attttaccat cagatatggg cggggactat gatgcgttag aaacattaat ggcgtcaaga
     6361 cctgacattt taaaccataa tattgaaact gttcgtcgct taacaccgag agttcgtgcg
     6421 cgtgcgactt acgacagaac attagagttt ttacgtcgtt caaaagaatt acaaccggat
     6481 atcccaacta aatcaagtat tatggttgga ttaggtgaaa ctatagaaga aatttatgaa
     6541 acgatggatg atttacgtgc gaatgatgta gatattttaa cgattggtca atatttacaa
     6601 ccttcacgta aacatttaaa ggttcaaaaa tattacacgc ctttagagtt tggtaaatta
     6661 agaaaagtgg caatggataa agggtttaaa cattgccaag ctggaccttt agtacgtagt
     6721 tcttatcatg cggatgagca agtaaatgaa gctgctaaag aaaagcaacg ccaaggtgag
     6781 gcacagttaa atagttaata tttaaccatt aataaggcat aaaggcttag tttgtacaaa
     6841 acgaacgtgt catagaagta atcattattt ttatgaaaca ctaaaagtat aaactgagcc
     6901 tttcattgtc atagataggt gaagaatttg ataaaagtag atcaacatta ctttgaatta
     6961 atagaaaatt atcgcgaatg ttttaatgaa gaacaattta ttgctaggta ttcagatatt
     7021 ttagataaat atgattacat agttggtgac tatggttacg atcaattacg attaaaaggt
     7081 ttttacaaag attctaataa aaaagcagag atgagtaaac gtttttcaaa tattcaagat
     7141 tacatatttg aatattgtaa ctttggttgt ccttactttg tattaagaca tttgtctaaa
     7201 caagaggtta aaaagttaat cgaagaagtt catccgtctg atgtgataga tgacgacaat
     7261 aaacttcaag atgtgaagat taagccaacc attcaagata ctgaacatta ataaaaccct
     7321 tagctagatt gaaaatggga atcatgcaat tcaagcatgg acctgtaatc tagttagggg
     7381 tttttatctt taatgaatga cttcatttaa atactcagta atttcatcgc cttcttcagc
     7441 atttacacct aaaatatgag cgatatagcc ttcttctttt aaatcatcag taccgataat
     7501 accgaattta tttgtttgca tattaagtac gagtgtctta ccataatgtc tatttgtatg
     7561 gactaacatc aaatcatatc gactatgctc gccaacaaaa ccaacaaact gaacttgact
     7621 ctcttcgttg tcatcatata aatacatatc aatcattttg tagcgactcc ttttaaaagt
     7681 agtaaagtta gtataacgac aaatgaagta tactgcaaaa ttatgataat atataagtga
     7741 gaggtgacaa ggaatgtatt ttgtagacaa agataaacta actcagaaat tagcctattt
     7801 acaagcatta actgatgatt atcatgagag caagcacaat cattatgcat ttgaacgcat
     7861 tgctcaaatg ttgatagaat catcggtaga tatagggaat atgattatcg atgcatttat
     7921 tttaagggat cctggtaatt ataaagatgt gattgatata ttagaactag aaaatgttat
     7981 tactaaagaa acacagcagg cgattaataa aactgtcggt attcgtaaac aatttacata
     8041 tgattacaca gccttagata ttaagattat catgccaatg tttgatgacg cattacctta
     8101 ttacaaacaa tttattacag aagtaacgac atttttacat caagaaaatg taccagtaac
     8161 agcttttggt aaaggagaaa atcaataatg aaacagtata aagcgtattt aatcgattta
     8221 gatggcacaa tgtatatggg aacagatgag attgatggag caaaacaatt catcgattat
     8281 ttaaatgtaa aaggcattcc tcatttatac gtaactaata attcaacaaa aacacctgag
     8341 caagtaactg aaaaattacg tgaaatgcac attgatgcta aaccagaaga ggttgtaacg
     8401 tcagcgttag ccactgctga ttatatttca gaacaatcac caggagcatc agtatatatg
     8461 ttaggtggga gtggtttaaa tactgcgtta accgaagcgg gacttgtcat taaaaatgac
     8521 gagcatgttg attatgtagt tattggactt gacgaacaag ttacatatga aaagcttgcg
     8581 attgcaacgt taggtgtaag aaatggtgca acatttattt ctacaaatcc tgatgtatca
     8641 attcctaaag agcgtggttt attacctggt aatggtgcta ttacaagtgt tgtaagtgta
     8701 tcgacaggtg tatcgccaca atttattggt aaaccagaac cgattattat ggttaaagca
     8761 ttagaaattt taggattaga taaatccgaa gttgctatgg taggcgattt gtacgatacc
     8821 gatattatgt ctggtattaa cgtaggtatg gatacgattc atgtacaaac aggtgtatct
     8881 acgttagaag atgtgcaaaa taaaaatgtg ccaccaacgt attcttttaa agatttaaat
     8941 gaagcaatag ctgaattaga aaaatagata tagtcatttt ataaagtagg tgaattgatt
     9001 tggtaaaaat agttgtttcg aggaaaattc cagataaatt ttatcaacaa ttaagtaaac
     9061 ttggtgacgt tgttatgtgg caaaaatcat tagtgcctat gcctaaagat caatttgtga
     9121 cagcacttcg tgacgcagat gcttgtttta ttacattaag tgaacagatc gatgcagaaa
     9181 ttttagcgca atcaccaaat ttaaaagtaa ttgcgaatat ggctgtagga tatgacaaca
     9241 tcgatgttga aagtgcaaca gcgaataacg tggttgtcac gaatacacca aatgtactta
     9301 ctgaaacaac tgcagaatta ggatttacat taatgcttgc tatagcacgc cgtattgtag
     9361 aagctgaaaa atatgtagaa gcagatgcat ggcaaagctg gggtccttat ttattgtcag
     9421 gtaaagatgt cttcaattca actattggaa tatatggtat gggagatatt ggtaaagctt
     9481 ttgcaagaag gttgcaaggg tttaatacta atattcttta tcataatcga tcaagacata
     9541 aagatgcaga ggcggacttt aatgcaacat atgtttcttt tgaaacgttg ttagcagaaa
     9601 gtgattttat catctgtaca gcgccactta caaaagaaac acatcataaa tttaatgctg
     9661 aagcatttga acaaatgaaa aatgatgcaa tttttattaa tatcggtaga ggacaaattg
     9721 tagatgaaac agcattaatc gatgcactag acaataaaga aattttagca tgtggtttag
     9781 atgtattagc aaatgaaccg attgatcata cacatccatt aatgggacgt gataatgttc
     9841 tgattacacc acacattggt agcgcatcag taacaacacg ggacaatatg attcaattat
     9901 gtattaataa tatagaagcg gttatgacaa atcaggtacc acatactcca gtaaattgaa
     9961 aataagtatg gtttttaatc attggtaaaa caagcaaagc aattgttaaa tatgttatgc
    10021 ttgacttgcg aatgtatata tttgcgctga tgataattca ataacattat aaactcgtca
    10081 tattaatgtg atgagtttat ttgattttga cgtatataac aaaattgtta tatagactgt
    10141 aattgtttat tgtttaaata acctttaaaa tgaatgatgt tctcaacaaa gtgtatcatt
    10201 tacaataatt gagtgatgac aaccaattac tatgttgaat ctataaaatt aaaattgaat
    10261 ttcattatga atatcgatat aatgaattgt aaaaaacaaa ttttaaaacg tattttatat
    10321 tgaacggtct ttataaaggc gtttttacta agaactttaa gaggtgcaat atgaaatcta
    10381 aaagtaaaca gccacctaat aaatatgttg aagcattcaa accatattta ttaacactat
    10441 tgtatttggc aatatttatt actttatatt taatttatgg cagtggcgac acacacaata
    10501 acttcattta taatgagttc taatgaggga gacttaatat gacagatatt attaacaagc
    10561 tgcaagcgtt tgcggatgca aatccacaaa gcattgctgt tagacacaca actgatgaat
    10621 taacttatca acagttaatg gatgagtcta gtaaattagc acatcgatta caaggtagta
    10681 agaaaccgat gattttattc ggtcacatgt caccatatat gattgttggg atgattggtg
    10741 ccattaaagc aggatgtgga tatgtacctg tagacacttc aattcctgaa gaccgtatta
    10801 aaatgattat taacaaggtt caaccagagt ttgtatttaa tacgactgat gaatcatttg
    10861 aaagtttaga aggcgaagta tttacaatag aagatattaa aacatctcaa gacccagtaa
    10921 tttttgatag tcagattaaa gataacgaca cagtatacac aatctttaca tctggttcta
    10981 ctggggaacc taaaggtgtt caaattgaat atgcaagttt agttcaattt actgagtgga
    11041 tgttagaact taataaatca ggaaatgaac aacaatggct taaccaagcg ccattttcat
    11101 ttgatttatc tgtaatggct atttatccat gtttagcatc aggcggtaca ttaaatcttg
    11161 tagataaaaa catgattaat aaacctaaat tattaaatga aatgctaaca gcaacaccga
    11221 ttaacatttg ggtatcaaca ccatcattta tggaaatgtg tttattatta ccaacgctta
    11281 atgaagaaca atatggtagt cttaacgaat tcttcttctg tggtgaaatt ctacctcaca
    11341 gagcagcaaa agcgttagta aaccgtttcc caagtgcgac gatttacaac acatatggtc
    11401 caactgaagc tacggtagca gttacaagta ttcaaattac acaagaaatc ttagatcaat
    11461 atccgacatt acctgttggc gttgaaagac caggcgcaag attatctact acagatgaag
    11521 gtgaacttgt tatcgaaggt caaagtgtaa gtttaggata cttaaaaaat gaccaaaaaa
    11581 cagctgaagt atttaatttc gatgacggta ttcgtacata tcacactggt gataaagcga
    11641 agtttgaaaa tggtcaatgg ttcattcaag gtcgtattga tttccaaatc aaattgaatg
    11701 gctacagaat ggaattagaa gaaattgaaa cacaattacg ccagtctgag ttcgtaaaag
    11761 aagcgattgt tgtacctgta tataaaaatg ataaagttat tcatttaatt ggtgcaattg
    11821 tgccaacgac tgaagttacg gacaatgcag aaatgactaa aaatattaaa aatgacttga
    11881 aatcacgctt accagagtat atgattccta gaaagtttga atggatggaa caattgccat
    11941 tgacttcaaa tggtaaaatt gacagaaaga aaattgcaga ggtaattaac ggatgattcc
    12001 atatggtgat tttacattct tcttaattgc tttaattgca ttattaccag tcattatact
    12061 tggattttta ggtaagcgaa gttacattta taatggcgta gttacagcat ttatgattgt
    12121 gttaatcttt tcttctgata aacataatct gtttgaccaa aagtatttaa gtgttcaatt
    12181 aattagtttt attatttacg tcgtatggca agttttattg ataatgtttt attatcattc
    12241 aaaaccaaaa aataattcat tttcaaaatt tgtaactgta atggttttat caatattgcc
    12301 attagcactt gtgaaagtgt tacaaagtac atggttaggt ggacatcaga ttcacttcca
    12361 tgaaagtaaa ttaattgaat ttgttggttt cttaggaatt tcttatgtta cattcaaaag
    12421 tgtgcagtta attatggaaa ttcgtgatgg ttctatcaaa gaaattaaag tatggaaatt
    12481 aattcaattt atttcattct tcccaacgat ttcatctgga ccaatcgatc gttacaaacg
    12541 tttcgttaaa gacgataaaa aagtaccaac aggcaatgaa tatcgtgaat tagtattaaa
    12601 agcaattcac atgattatgc ttggtttctt gtataaatat attgttgctt actttattaa
    12661 cacatatgca atcatgccgt tacaattaga cttacatggc tttgtcaatt tgtggttata
    12721 tatgtacgca tacagcttat atttattctt tgactttgca ggttatagtt tatttgcgat
    12781 agcatttagt tatttattcg gtattaaaac accaccaaac ttcgataaac ctttcaaagc
    12841 gaaaaatatt aaagatttct ggaatagatg gcatatgaca ttatcattct ggttcagaga
    12901 ttgtatttac atgagatctt tattctacat gtctcgtaaa aaattattga agagtcaatt
    12961 tgcaatgtct aacgtggcat tcttaatcaa cttcttcata atgggaattt ggcatggtat
    13021 cgaagtgtat tacattgttt atggtttata ccatgcagca ttgtttatag gttatggcta
    13081 ttatgaacgt tggcgtaaga aacatccgcc acgttggcaa aatggtttca caacagcact
    13141 tagcattgtg attacattcc actttgtaac atttggcttt ttaatcttct caggtaaact
    13201 tatataataa aggagaattt aattatggaa tttagagaac aagtattaaa tttattagca
    13261 gaagtagcag aaaatgatat tgtaaaagaa aatccagacg tagaaatttt tgaagaaggt
    13321 attattgatt ctttccaaac agttggatta ttattagaga ttcaaaataa acttgatatc
    13381 gaagtatcta ttatggactt tgatagagat gagtgggcaa caccaaataa aatcgttgaa
    13441 gcattagaag agttacgatg aaattaaaac cttttttacc cattttaatt agtggagcgg
    13501 tattcattgt ctttctatta ttacctgcta gttggtttac aggattagta aatgaaaaga
    13561 ctgtagaaga taatagaact tcattgacag atcaagtact aaaaggcaca ctcattcaag
    13621 ataagttata cgaatcaaac aagtattatc ctatatacgg ctctagtgaa ttaggtaaag
    13681 atgacccatt taatcctgca attgcattaa ataagcataa cgccaacaaa aaagcattct
    13741 tattaggtgc tggtggttct acagacttaa ttaacgcagt tgaacttgca tcacagtatg
    13801 ataaattaaa aggtaagaaa ttaacattta ttatttcacc acaatggttt acaaaccatg
    13861 gtttaacgaa tcaaaacttt gatgctcgta tgtctcaaac tcaaattaat caaatgttcc
    13921 agcagaaaaa catgtctact gaattaaaac gtcgttatgc acaacgttta ttacagtttc
    13981 cacatgtaca caataaagaa tacttgaaat cttatgctaa aaaccctaaa gaaactaaag
    14041 atagttatat ttctggtttt aaagagaatc aattgattaa aatagaagcg attaaatcat
    14101 tgtttgcaat ggataaatct ccattagaac atgttaaacc tgctacaaaa ccagacgctt
    14161 cttgggatga gatgaaacaa aaagcagttg aaattggtaa agctgatact acatcgaata
    14221 aatttggtat tagagatcaa tactggaaat taattcaaga aagtaagcgt aaagttagac
    14281 gtgactacga attcaatgtt aattctccag aattccaaga tttagaatta cttgtaaaaa
    14341 caatgcgtgc tgctggtgca gatgttcaat atgtaagtat tccatcaaac ggtgtatggt
    14401 atgaccacat tggtatcgat aaagaacgtc gtcaagcagt ttataaaaaa atccattcta
    14461 ctgttgtaga taatggtggt aaaatttacg atatgactga taaagaatat gaaaaatatg
    14521 ttatcagtga tgccgtacac atcggttgga aaggttgggt ttatatggat gagcaaattg
    14581 cgaaacatat gaaaggtgaa ccacaacctg aagtagataa acctaaaaat taaaatacaa
    14641 atagcacata actcaacgat tttgattgag cgtatgtgct atttttatat tttaaatttc
    14701 atagaataga atagtaatat gtgcttggat atgtggcaat aataaaataa ttaatcagat
    14761 aaatagtata aaataacttt cccatcagtc caatttgaca gcgaaaaaag acaggtaata
    14821 actgattata aataattcag tattcctgtc tttgttgtta ttcataatat gttctgttaa
    14881 cttaatatct ttatattaga atacttgttc tacttctatt acaccaggca cttcttcgtg
    14941 taatgcacgc tcaataccag ctttaagagt gattgtagaa cttgggcatg taccacatgc
    15001 accatgtaat tgtaatttaa caataccgtc ttccacgtca atcaatgagc agtcgccacc
    15061 atcacgtaat aaaaatggac gaagacgttc aataacttct gctacttgat caaacatcgt
    15121 tgtatcttca gtaggcatca ggcaagtctc ctttcgagag atgaatttga taatctttat
    15181 tagtatatta taatagatag tgtaataaaa atctatcaaa ccaatggggg ataaatatat
    15241 ggagcacgtg agtgtggtag tttatggggc agatgttata tgtgcaagtt gcgttaatgc
    15301 gccaacatcg aaagatattt atgactggct acagccgcta ttaaaaagaa aatacccaaa
    15361 tatatcattt aaatatacgt atatagatat tacaaaagat aatgacaact taacagatca
    15421 tgatttacaa tttattgaaa gaatagaaca agatgaacta ttttatccat taattacaat
    15481 gaacgatgaa tatgtagcag atggttatat acaaacaaag caaatcactc gatttataga
    15541 ccaaaagctt gtgaatgaat aatatacaca taactaaaag caatgtcagt aatagtgtga
    15601 actgacattg ctttttaatt ttaaccattg tgatatttat ataaccaaag tacgccggat
    15661 tttaaaatag aagcgagtcg tcctgttaca gtgcggtcca tgatatatgc aaaaccttgc
    15721 ttatctccta atgacccaac gataccttgt acctttagtt ccggcatttt gtcaggtaat
    15781 ggttcattta gccattgctt tttaagcaca tcggcaattt gatcaccttg aacttcggct
    15841 aactgagcac ttggcgcatg tggtaaatca gcacaatcac caactacata gacgttacga
    15901 tatgttggta cttgatgata ctggttaact atcacgcgtc cattactatt tatatcaatc
    15961 ggcaagttac gaacaacttc aacaggttga attcctgctg tccatacaac taaatcaata
    16021 tctttaggtt catcacagtt atatatttta ccaggttcaa ctttattaat atttgaattt
    16081 ggaacaacgg taacattatt tttggcgaac catttcgcaa catacttact taatttttct
    16141 ggaaaatttc ttaaaattcg cggcccacgg tcataaagat atatttccaa gtctgatcta
    16201 ctttctctta attcgctggc aagttctatg ccgcttaatc cagcaccaac gataccgact
    16261 ttagcacctt ctggtagttc actaatacta tggaaagtat cccgagcctt tgagagtgtt
    16321 tgaatactat gtgtatattc ttcggctcct ggaacgttat gatatttatc ttcacatcct
    16381 aaaccaatga ttagctcatc ataatcaatt ttagaattac cgactgagac aatttgagca
    16441 tctaaatcta tgtcgttaat ttcaccataa actgtattca cttgtggatg attagggaat
    16501 ttcatacgaa catctttatc tgatttcgtg cccgcagcta aagcataaaa ttctggtttc
    16561 aatccatgaa atggcatacg atcaactaat gtgactgtat aatcttgtgg taaagaagta
    16621 gttaaaatgc gtgacatgat acgcatatta ccatatccgc cgcctaacaa aactaagttt
    16681 ttcataatga agtacccctt ttatatgtta atatttactt taattttgaa aagatcaaag
    16741 tgcatagata tatttgttaa tgaacagtta taaaatgcaa gtgtctttga tt
//
LOCUS       DAMQYT010000034        16587 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMQYT010000034 DAMQYT010000000
VERSION     DAMQYT010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16587
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.034"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..564
                     /locus_tag="PGA33_000914"
     CDS             <1..564
                     /locus_tag="PGA33_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HDG6472743.1"
                     /translation="DIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKK
                     DDKDLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQA
                     MFIYELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFE
                     EEYKKEQEREKEKEKQKDDDHSGLDEV"
     gene            649..902
                     /locus_tag="PGA33_000915"
                     /pseudo
     CDS             649..902
                     /locus_tag="PGA33_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            918..1241
                     /locus_tag="PGA33_000916"
     CDS             918..1241
                     /locus_tag="PGA33_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472744.1"
                     /translation="MNNEELEMRLLLMKQSIEQLQEELAPNLKTRDLVLLRYMYSYKE
                     INMLDSYLFQLATNKEQITKKQFKTKLENIREVPEIPIRQVNDILEGYKNSELYVELI
                     NSILK"
     gene            complement(2111..2472)
                     /gene="ssrA"
                     /locus_tag="PGA33_000917"
     tmRNA           complement(2111..2472)
                     /gene="ssrA"
                     /locus_tag="PGA33_000917"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2563..3027)
                     /gene="smpB"
                     /locus_tag="PGA33_000918"
     CDS             complement(2563..3027)
                     /gene="smpB"
                     /locus_tag="PGA33_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDG6472745.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            complement(3049..5421)
                     /gene="rnr"
                     /locus_tag="PGA33_000919"
     CDS             complement(3049..5421)
                     /gene="rnr"
                     /locus_tag="PGA33_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDG6472746.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSYRGQSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEGSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPNVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     NEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGRKKKGKQRKGKNQRNNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            complement(5455..6195)
                     /locus_tag="PGA33_000920"
     CDS             complement(5455..6195)
                     /locus_tag="PGA33_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HDG6472747.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPDEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDNEMDHFKPTET
                     LKELSEALDTIKEQVDEVLDPILVIQAENDNMIDPQSANYIYDHVDSDDKNIKWYSES
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            complement(6311..6544)
                     /gene="secG"
                     /locus_tag="PGA33_000921"
     CDS             complement(6311..6544)
                     /gene="secG"
                     /locus_tag="PGA33_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDG6472748.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            complement(6611..7069)
                     /locus_tag="PGA33_000922"
     CDS             complement(6611..7069)
                     /locus_tag="PGA33_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472749.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            complement(7409..8713)
                     /gene="eno"
                     /locus_tag="PGA33_000923"
     CDS             complement(7409..8713)
                     /gene="eno"
                     /locus_tag="PGA33_000923"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDG6472750.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
     gene            complement(8843..10360)
                     /locus_tag="PGA33_000924"
     CDS             complement(8843..10360)
                     /locus_tag="PGA33_000924"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDG6472751.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            complement(10363..11124)
                     /locus_tag="PGA33_000925"
     CDS             complement(10363..11124)
                     /locus_tag="PGA33_000925"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDG6472752.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            complement(11246..12436)
                     /locus_tag="PGA33_000926"
     CDS             complement(11246..12436)
                     /locus_tag="PGA33_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDG6472753.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            complement(12575..13585)
                     /gene="gap"
                     /locus_tag="PGA33_000927"
     CDS             complement(12575..13585)
                     /gene="gap"
                     /locus_tag="PGA33_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDG6472754.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            complement(13638..14651)
                     /locus_tag="PGA33_000928"
     CDS             complement(13638..14651)
                     /locus_tag="PGA33_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDG6472755.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            complement(15411..16040)
                     /locus_tag="PGA33_000929"
     CDS             complement(15411..16040)
                     /locus_tag="PGA33_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HDG6472756.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
BASE COUNT         5171 a         3141 c         2498 g         5777 t
ORIGIN      
        1 gatataaaaa ttaaaggcga tactattgtt agtgacaaat ttgaagctaa aataaaagaa
       61 ccgtttatca tcaatgaaaa agatgagaaa aagaaatata tcgcttttaa aatggaaatt
      121 actgctaaaa aagacgataa agatttaaat ccatcttcta tttctcatga ctatattaat
      181 atcactcaag atgataaaaa tacagtaaat aaattaagag atggttatct tttaagtgat
      241 aaaaaatata aagattggac agaacataac caagatcaaa ttaaaaaagg caaaactgca
      301 caagccatgt ttatctatga gttaagaggt gatggaaata ttaatttaaa tgtccataaa
      361 tactcagaag ataaaacagt tgattctaaa tcattcaaat ttagtaaact taaaaccgaa
      421 gatttttctc atagagcgga aacaagagaa gaagtagaaa agaaagaaaa agaatttgaa
      481 gaagagtaca aaaaagaaca agaacgagag aaagaaaaag aaaagcaaaa agatgacgac
      541 cacagtggtt tagatgaagt ataaatttgg tttgttgatt gtcttacatt cagttattta
      601 atttttagag aaactgactt atttatagct tagccaacat gatactataa agctatgatt
      661 aaaaataaaa aatctgtgtt taatggtgtt aaagaaatca gtccccgtgc cggaaaatca
      721 taaaaaaaag atgggataaa tttgtaaatc ccgcattgaa taatgcatta aaacaagaag
      781 aggctacatg gggaaatgta caaggtcaag tttcacaagc attgatggga actggtatta
      841 aagattctac tgcaagaagc atagggtttt gggtttccca agttggtcaa gcattaatat
      901 aaaaagagtg tgataaaatg aataatgaag aattagaaat gagattgtta ttaatgaaac
      961 aatcaataga acaattacaa gaagaactag cgcctaactt aaaaactaga gatttagtgt
     1021 tattaagata tatgtattca tataaagaaa ttaatatgct agattcttac ttgttccaat
     1081 tggctacaaa taaagagcaa ataacaaaaa aacaatttaa aacaaaattg gaaaacatta
     1141 gagaagtacc agaaatacct attagacaag ttaatgatat attagaaggt tataaaaata
     1201 gtgagttata tgttgagttg ataaatagca tcctaaaata aaaataaaca tactaagatt
     1261 agctatgaag gaatctatga cgatagattt tttcatagct attttttata gttataaaga
     1321 ggagtagtct gttctgactc ttggatttta aatatgtata ataaacaagt aagctttact
     1381 ctcgcctttt aaaattcgtt tgtagtatgt tgggttctta aaatcgtgaa taggaaaatg
     1441 caatgagaaa ggttaagtaa agtttttaac ttctcattta ttcaatggaa aattttttat
     1501 cgattactta gacattaata ttagtaaaag aagtagtgta gttgcaaatt ataaaaacta
     1561 aaaattccaa aaagagttta tcatccaaaa caataaaaat ggctacaatg gtatgttgtg
     1621 aaatgcattt cttaactacg acttctttta tttccgtctc gtcaactatt gctgattgtg
     1681 ttaataggca tattgattct aatgaaaata tcaaaactcg gctcatctct ttataactac
     1741 acccacttag tgacgtaaca aaaatgaaaa gttctttttc agtagccatt tatccaatta
     1801 agtgtagacg attcacattt ttatattgac atatataaca tggagaattt tttgcgtata
     1861 aagaagtttc ttcatgaaca atgttgaatg taaaactcag aatccaactt ttttgacact
     1921 tatttgacac acatcactgt ccccttatat ctcaaaaact aattcataat cacttattac
     1981 tctttcctcc atcaaataaa cacctataca aacactgcta tatcaatact cacaacaaat
     2041 tctgcaatgc attatcccct gaaaaacaca aaataaaaaa gcccacaacc acaaaggctg
     2101 taggctacaa atatggagac ggcgggattt gaacccgcgt ccaaaggtcc tgatacacat
     2161 ctttctacgt gtgtagttta tcgataggtt tcgcatcatg aaaagtgata aacaaccaac
     2221 atgatgctag tttgattaag tttcttctaa acagacttca aacggcagtg tttagcatat
     2281 cctattaagg ttgaatcgcg ttaacagcac ataggaaatg ctgttaggcg atgcagagtg
     2341 cgattaggca gctactgcga aattattgtt tgatttgcca gttattataa actgtgtgtg
     2401 ttgatgacga agacaaccct ccgacacgct taatgagctc gggggacccc tgtcgaatcc
     2461 atgaacgtcc ccgaaataat aaatcaaaca aatcatactt ttgtaattta caaaagcaca
     2521 tataactata tcaaatatag cccgattaag caactaaatc gcttaataac gggctttcat
     2581 atcgcgcgca acatctcgtt tgactgcttt ttctttcaaa gcttgacgtt tatcatattt
     2641 tttcttacct cgtgcaacac caagtaatac tttacaatgt ccatgcttca aataaagctt
     2701 taacggcaca atcgaataac caatctcacg tgtttgatca cccaatttaa tgatttcacg
     2761 cttgtgcaat aataattttc gagaacgaag aggatcgtga ttaaaacgat tcccttcttc
     2821 gtatggtgct atatgcatat tattcaaata catttcaccg tttttaactt gcgcataact
     2881 atctttaagg ttagcactac ctcggcgaat tgattttatt tctgtgcctt gcaatacaat
     2941 tcccgcttca atcgtatctt caatattata atcatgtctt gccttacgat tttccgctaa
     3001 tgtacctggt gatttcttct tagccattca tactcacctc attgctgctt attttttctt
     3061 acgacgtgct ttctttttca cacttttatc tttataaaat ggcttatgct tactattacc
     3121 tgatttatca ttattacgtt ggtttttacc tttacgttgc ttaccttttt tcttacgacc
     3181 cttatcatca gattttgatt tatctaatga tttaccacgc gttttggctt gaattgtctt
     3241 acctcgcgct gggcgctgtg atcgatcatt tttcggtaaa ggcataccta caatttgaaa
     3301 atcaattaat cgttcatcta catcaacatg cgtcacttta accttaactg tgtcaccaat
     3361 tctaaatact ttagcttgac gctcaccaat taatgccatt tgacgctctt caaaacggta
     3421 ataatcatca gtcatattcg caatatgaac cataccttct atcgtatttg gcaattcaat
     3481 gaacataccg aagttagcta ctgagctgac aataccttca aattcatcac caatatgttg
     3541 aatcatatat tctgcttttt tcaattcatc agtatcacgt tctgcctcaa tagcacgacg
     3601 ttcacgttta gaagtatgtt cagctaactc aggcaatttg tcttcccaac gcttcacttc
     3661 tttgttatcc attgatttct caattaaata cttacggatt aaacgatgaa ctgttaaatc
     3721 aggataacgt ctaattggtg atgtaaaatg cgtataatat tcagctgata agccaaaatg
     3781 tcccaagttc acatcatcat aatgcgcttg ttgcattgaa cgcaacatca ttgttgaaat
     3841 gaccatttgt tcaggtcgac cttctacttc ttcttgaacc ttttgaagtg ttgttggatg
     3901 aatatcttcg ccagtaccct taatcatgat gccaaagttt gtaataaaat caaagaattg
     3961 tcttaagcga tctgatttag gttgctcatg cactcggtaa ataaaaggta catctaactt
     4021 actaaaatgt tcagcaactg tttcatttgc aattaacata aatgattcaa ttagacgttc
     4081 accctcgcca cgttgtctta attgaacatc tgttggtata ccgtcttcgt taactaatac
     4141 ttttgcttca ctaatatcaa aatcgatttc accacgtcgt tttctcattt gaatcaaacg
     4201 attagataaa tcttgtgcta aatctagcat aggcgtaatt tcattatatt gttcgcgaat
     4261 gttaggatcc ttttcagtaa taatctgatt taccgcatca tacgtcattc gataatcaga
     4321 atgtataaca ctatcaaaaa tttcatgttt aacaacgcga ccactagcat cgatttccat
     4381 gcgacagctt agagttaaac gatcaacatt aggattcaat gaacaaatac cattacttaa
     4441 tcgatgtgga atcattggaa ttacacggtc aacaagatat acacttgtcg ctctatcata
     4501 tgcctcttta tccaatgcag aaccttctgt tacataatag ctgacatcag caatacttac
     4561 agttaattgc gtattaccgt tcgctaactt tttaacacta attgcgtcat ctaagtcttt
     4621 agcatcagca ccatcaattg tgattgtcaa ttcatcacgt aaatcatgac ggcctttaat
     4681 ttcagtattt tcaatatgat caggtactgc ttcagcttct tgtaacactt catcaggaaa
     4741 ttcaatttct atgccatgtt gatagataat agataaaata tctacgccag gatcattttt
     4801 atgtcctaaa atagcagaaa tatgtccttc tggattatct gaaccatcag catacttagt
     4861 aatttgtaca agtaccttat gaccatcgac tgcgcctaaa ctttgacctt taggaatgaa
     4921 aatatcttgc ataatacgtt tatcatccgg aataacaaag ccaaaatgtc tagcttcact
     4981 atacgtacca acaacttgag ttacagaatg cttctcaatc gacttaactt ccccttcgat
     5041 tttaccttta tgttcacctt ttgattgatg gatttctaca ataacagtat ctccatccaa
     5101 ggcacgatta attttcgtcg ggggaataaa tatatcttcc atatcctcat cttcaggtct
     5161 taagaatgca aagccttttt tattttgact taacgttcct tttatcaatt ttgattgacc
     5221 tctataacta tgcttttttt ggtatctgtc tgtttttgta cgttcgatta atcctgattg
     5281 ttctaactcc acaagcacct taattaaatc tctaaacgag tcggcactgc ttaaacctaa
     5341 tgcatcttga aaatctgaca ctgacatagg ttcatattca ggttgattaa taatctcttc
     5401 tatagattgc tttaaattca ttatgcccct cctttctaat gttaaaatct ctttttattc
     5461 tgaccagtct aatgactcta aaaattgata aatatcttca aatacttgtt ctttctcttt
     5521 atcaatcgta ataacatgtc cagattcact gtaccacttg atatttttgt catcagaatc
     5581 tacatggtca tatatataat ttgcggattg tggatcaatc atattgtcgt tttctgcttg
     5641 aatcactaaa ataggatcca acacttcatc aacttgctct ttaatcgtat ctaatgcttc
     5701 acttagttct tttaaagttt cagttggttt aaaatgatcc atttcattat caatagtctc
     5761 ttgatcttta ccttcatact ttttaaaatt gcgtgcatat tctaaaaagc cttcataaat
     5821 ggcaccttca gttttgccac ccataggagc acacatcgtt acaataccct ttacatctct
     5881 atttaagctt aattttaaag caaaatcccc acctaatgat agaccagcaa caacaatttc
     5941 atcataacct tgttcaacaa gataatcata accatctaac gcatctttaa accaaacgaa
     6001 aggactagat ttcagtattt catctggtgg tgccgcgtgg ccttcatatt gcggtgcata
     6061 tgatgtataa cctttctttt gtaaaaatcg acctaattga cgaacatcag acgaattgcc
     6121 tgtaaaacca tgtagtaata acacggcacg tttaccttcc tcaaaaaaga aaggttttgg
     6181 taattttatc tgcataaatc taacgctccc tttgcatact taactaggct atatatttta
     6241 catagcttat tgttaaacta ttacattctt aattataaat aaaaaagccc gacatttaca
     6301 tcgccggacc ttacatacca agataactta tgcaaatcat aagtacaaaa aataatattg
     6361 ataaaataat tgttaatcta tttaagaata aatcgacgcc acgttgtttt tgtttaccga
     6421 ataactgctc agcaccacca ctgatggcac ctgaaagtcc actgctttta ccttcttgga
     6481 gtagtacaac agttattaat gcaatacaat caatgattaa taatacgatt aaaaatgtat
     6541 gcataaattg tcctccgttc cttatatacg acaagtagta tatcatattt ttattgttaa
     6601 aatcaatttt ctagcgatta aatgtacttt tacgtttgat agaaacaaca agcatataaa
     6661 tcgctaatgc aaccgaacct ataataacaa ttaaaattgg caatgttaag tacgcaccgt
     6721 gtgcagcttt tattgcttga attgtgattg ataaattaat caacgtaaac aaactatttg
     6781 atacataaac tgcaaacata aaccaccaca cataagtatg tcgattccaa aaagtgatga
     6841 agcctgctag ataagccaaa atatacataa taccagtgaa tctaaagctg tttaaaatgt
     6901 aatcaatcgc tttatctgaa gtgttttgat tcgttaaatt aagtaatgct ttggcagtcg
     6961 cattatccaa cgaaacaaag taatgaataa tcaataatat tgagaatacg attgatgcag
     7021 ttgcaataaa gcgtcctgtt ttaatttctt tattcgttct atcagccatt tgtttactcc
     7081 tcatatattt aaatacgttc tataaaacct ttatttaatt ttacctttta ataaaagtga
     7141 ataccctgtc atacaatatt catttcaaca taaatttatt ataccagttt aagaatacaa
     7201 gttaaatatg tgttattagt tacctcatga ttattagtct gttatgatta atacttcaaa
     7261 ataaaaaacc taccacagtc tcaactctgt ggcaggctat aaatttaagc gtgaacttat
     7321 ttaagcgtga actcaaaatc tttatttaat ttataccgtc ataataatcc tcaactaaaa
     7381 ttatgtcagc atttgattat aaagaaaatt atttatctaa gttatagaat gatttgatac
     7441 cgtcatattt agcagtttca aataattcat cttcgatacg taataattga ttgtatttag
     7501 caatacggtc agtacgtgat aatgaaccag ttttaatttg accagcgttt gtagcaacag
     7561 caatatcagc aattgttgta tcttctgttt cacctgaacg gtgagaaact actgctgtgt
     7621 aaccagcttt ttgagccatt tcgattgcat caaatgtttc agttaatgta ccgatttggt
     7681 taactttaat taagattgag ttaccaattc cgttttcaat accttttgct aaaatttcag
     7741 tgtttgttac gaataaatcg tcacctacta attgtacacg gtcaccgata cgttctgtaa
     7801 gttgtttcca accatcccag tcgttttcgt ccataccgtc ttcaattgta atgataggat
     7861 atttgtctac taattgttct aagtagtcaa cttgttctgc agctgtacgt tttgcaccgt
     7921 gttcgccttc gaacttactg tagtcatata caccattttc atagaattct gatgatgcac
     7981 agtcaaatcc taagaatact tcttcacctg gtttgtaacc agctgcttcg attgcttgga
     8041 taattgtttc aacagcatct tcagtacctt caaatttagg agcgaaacca ccttcgtcac
     8101 ctactgcagt ttctaaacca cgtttgctta aaattgattt taagttgtgg aaaatttcag
     8161 taccccaacg taatgattct ttgaacgttg tagcacctac aggtaaaatc atgaattctt
     8221 ggaatgcaat tggagcatct gagtgagaac caccattaac gatgttcatc attggtactg
     8281 gtaactgctt accattaaat ccacctaaat atttgtaaag tggttgacct aataagtcag
     8341 ctgctgcacg tgctactgcg atagatacac ctaaaatagc atttgcacct aatttacctt
     8401 tgtttggagt accgtctaat gcgatcatca ttttatcaat agatacttga tctaatactg
     8461 aaaattcacc ttcaataatt tctggtgcga tgatttcatt aacgttttca actgctttag
     8521 taacaccttt acctaaataa cgtgatttgt ctccatcacg taattcaaca gcttcgtgtt
     8581 caccagttga agcacctgat ggtactaatg cacgaccaaa tgcgccactt tcagttaata
     8641 cttctacttc aacagttggg ttaccacgag agtctaagac ttcgcgagcg taaacatctg
     8701 taataattgg catgtttata atctccttta aatagttatt ctataattat tatagcttta
     8761 ttctttcatt taacaaaatg atattactat tattttcaaa aaataagtgt cattaagcgt
     8821 ttacttaaca tcttttacaa tattagtgtt taattaaaga ttcacctgtc atatcttcag
     8881 gttgttctac atttaataaa tctaataatg taggtgctaa gtcacctaag cgaccagttt
     8941 ctctaagtgt aacgccttct tttgttacaa tcactggtac tgggttcgtt gtatgcgtag
     9001 tcattggttg atcatcatcc gtcaatactt gatcagagtt accatggtca gcagtaataa
     9061 ttgcataacc gtccatgtct aaaatcttat caaccacttc tcctaaacat tcatcaaccg
     9121 cttcgattgc tttgattgtc ggctcaagca taccactatg tccaaccata tcagggttag
     9181 caaagtttaa aataattaag tccaagtcac ctttatttaa ctcttctaat aatgcatctt
     9241 taacttcata agcactcatt tctggtttca agtcatacgt tgcaacttta ggtgaatcaa
     9301 ttaaacgacg gcgttcacct ttaaattcct cgttacgtcc accactcata aagtaagtaa
     9361 cgtgagggta tttttcagtt tctgcaatac gtaattgagt taaattgtta ttttgtgcaa
     9421 tttcaccaat tgtattattt aaatcaactt tttcgaagac gatagccgca tcgatattgt
     9481 cattatactt agtgaatgtt gcatagaata agtctttaac ttgttcaact ttaaagcctt
     9541 cgaatgctct gttcgcaaaa atttccgata attgcgctgc tctatcaggt cggaaattat
     9601 agaagatcac tgcatctcca tcattaacac cgtcattttg attctcaacg atgaatggta
     9661 ctacgaattc gtcagttaag ccctcattat agcttgcttc tacaccttct ttggcagttg
     9721 cataagttgg ggcatcaaaa ttacgaatag cattgtaagc tttttcttca cgttcccaac
     9781 gtttgtcacg atccattgca taataacgac cagacacaga tgcaaattga ccaatgccta
     9841 attcattgaa tttagcttca gtctcttcga tgtatttcaa agcggatttt tgatctacgt
     9901 cacggccatc taaaaatgcg tgtacgtaaa ctttttcaac accttgtttt ttagcaagtt
     9961 ctaacaaagc aaataaatgt ttgtaatgac tgtgtacacc accgtcagac aataaaccaa
    10021 agatgtgtaa cgctgaatca tgtgaattca cgtgtgcaat tgcattattt aaaacatcat
    10081 tttcaaagaa atcaccgtct tcaattgatt tattgattcg agttaaactt tgataaacga
    10141 tacgtcctgc accgatattc atatgaccaa cttctgagtt acccatttgt ccttcaggta
    10201 gtccaacatc taagccactc gcttcgattt gagtcgttgg atatttgttg taataacgat
    10261 caaaattagg cttgtttgct aattttaccg cattaccatg ttcgctttcg cggttcgcaa
    10321 aaccatctaa aataattaac gcagttggtt tcttagccat gattattttg caccttctaa
    10381 caattgtacg aaatcttcaa ctttaagtga tgcgccacct actaatgccc catcaatatc
    10441 agtttgtgcc atgtattctt taatgttgtt aggtttaaca ctaccaccat attgaatacg
    10501 agttgcttct gatacttctt tgcttgataa gtcagcaata gtttgacgta caaatgcaca
    10561 catttcattt gcatcttcag atgttgatga tttaccagtt ccgattgccc agattggttc
    10621 ataagcaatt acaactgatt taagttgatc ttcagataaa cctgcaacag ctttcttaac
    10681 ttgctcacct acaacatcgt tagctttacc actttcacgc tcttcgtctg tttcaccaac
    10741 acatataatt ggagtcattc catgtttgaa aatagcgtgc gcttttttgt taatttcttc
    10801 atctgtttcg tggaataatt cacgacgttc agaatgaccg ataacaacgt atttaacgcc
    10861 taaatctgct aatgcaactg gagacgtttc acctgtgaac gcaccattat cttcgaaata
    10921 cgtattttga gcaccgattt ctaaaccttg tgcttttcct tctttaactg cagtagttaa
    10981 tgcatctaat tgaattgctg gtgcacaaat tactgattct acttcttttg aatctggtaa
    11041 tgttggtaat gcattgacga agtcttttgc ttcttgtact gttttgttca ttttccagtt
    11101 accagctata attggtgttc tcattaaaga cactccttgt tttgtaaata tttttgaaaa
    11161 gtgatgaaac acgatgtcat cttgtgactg ttttcccgta acaatgttaa acaaacatgc
    11221 cacatcactt taaactatca ctttattatt tattattgat tgctttgata ccaggcaatt
    11281 ctttaccttc taggtactct aatgacgcgc cgccaccagt tgaaatatga gtgaagtcat
    11341 tttcaaaacc taaagagatt gctgctgcag ctgaatcacc gccaccgata atcgtaattg
    11401 catctttaag gtttgcaatt gctttacata caccaattgt accttgtgca aagttactga
    11461 actcgaatac acccataggt ccattccata caacagtgtg cgcaccttct aattcatctg
    11521 caaataattt tacagtgttt ggtccaatat ccataccttc ttggtctgct ggaattgaat
    11581 cagatggtac tacagtgatt ttggcatcat tagaaaattc tttagcaact ttagtgtcta
    11641 ctggtaatac aattttatca ccatgttttt ctaataaatc ttttgcgaag tcgattttat
    11701 cttcttctaa taatgaaata ccaatttctt taccttgcgc ttttaagaaa gtataagcca
    11761 tacctccgcc gatgataatt ttatcagcta tgttaactaa gtttttgatg acattaattt
    11821 tgtcagatac ttttgctcca cctaaaatag caacaactgg tttatgtgga tcgttaacta
    11881 cgccgccaat aaacttaatt tctttatcca ttaagaatcc agctgcagtt tctaaatgtg
    11941 tagaaatacc aacattagat gcatgctcac gatgcgcagt accaaaagca tcatttacaa
    12001 acacatcacc taaagatgcc cagtatttac ctaattctgg atcattttta gattcttttt
    12061 taccgtctaa atcttcataa cgtgtatttt caactaataa tacgtcgcct tctttaaggt
    12121 ctttaatagc agcttcaagt ttttcgccgc gtgtttctgg tacgaaaaca acttctttat
    12181 ctaatttctt agataagtct tcagcaactg gacgtaaagt taattttgct ttatcacttt
    12241 cttctttcac tttacctaaa tgtgaaaata gtacgatttt accaccttgt tcgatgatgt
    12301 attgaattgt aggtaaagct tgaacgatac ggttgtcatt agtaatttca ccgtctttta
    12361 aaggtacgtt aaaatcagca cgtactagga ctgttttacc tttaagatct aaatcagaaa
    12421 caattttttt agccatggtg tttcctcctt gtaattagga aattttatat aaaaataagc
    12481 ggagaagcgt ttgtgctccc cgcttactca taagctttaa ttaatgctaa atagttctga
    12541 gcactagcgt atttgaataa aaactatact aaaattattt agaaagttca gctaagtatg
    12601 ctaatgtacg aactaattgt gcagtatatg acatttcgtt atcataccaa gctgcaactt
    12661 taactaattg acggtcgcca actgacatta cacgagtttg tgtagcgtcg aataatgaac
    12721 cgtaagtcat acctacaacg tctgaagaaa cgatttcgtc ttcagtgtaa ccgaatgatt
    12781 cgtttgaagc atttttcata gcttcgttaa cttgttcaac tgttacgtct tgtttttcta
    12841 atactactgt taattcagtt aatgaacctg tagctacagg aacacgttgt gcaccaccat
    12901 ctaatttacc atcgatttca ggaataactt taccgatagc tttagcagca cctgttgagt
    12961 tagggatgat gttttctgcc gctgcacgag cacgacgttt gtcacctttt ctgtgaggtg
    13021 cgtcttgtgt attttgatca cctgtgtaag cgtgaattgt agtcattaaa ccttcaacta
    13081 aaccaaagtc atcgtttaaa actttagcaa ctggtgctaa tgagtttgta gtacatgaag
    13141 cacctgaaac aactgtttca gaaccgtcta actcttggtg gttagtgttg aatacgattg
    13201 tttttaagtc accagtagct ggtgctgaga ttaatacttt tttagcgcct gcttcaatat
    13261 gagcttgtgc tttatcttta tcagtgtaga aaccagtaca ttctaatact acatcgatat
    13321 ttaagtcttt ccaaggtaat ttgcttgcat ctggttcact gaatgattta acttctttac
    13381 catttacgcg gaaaccacca tcaactacct ctacttcacc tgtgaaacga ccttgcatag
    13441 tgtcatattt taataaatgc gctaacatgt cgtcatctgt taagtcgttt actgctacaa
    13501 cttcaagacc ttctacttct tgaattcttc tgaatgctaa acgaccaatt ctaccaaaac
    13561 cattaattgc tacttttact gccattataa tggcctcctt taaaatgata tttaaaaagt
    13621 attaaacttt ttatctctta ttcaagtatt atctttgctg cggcttcatc agtgattaac
    13681 actgtattct tgggtgcaat cgtcaagtat gctttaattg cttcaccttt cgatttgcct
    13741 cctgcaactg caaaaataaa gtcttttgat tcaaggtctt ctaattgaag tccaattgtt
    13801 ttaaccttat ggacaatttg accttgtgta tcaaaataat aaccaaatgc ctctccgaca
    13861 gcttgatgat gttgaagttg ttcaatgacc ttttcaggtg attgacgtcg atgcgccatc
    13921 ttcagcgcat caccaatgcc gtgtaatata acgtttgctt gtttaatttt gtctaaagtg
    13981 tttatgactg atggctctaa caacaatgta ttatatgttg tttcactgac attatcaggt
    14041 acatacatcg tcgtataata accgccagct tgttgtgcca tactggctgc aattgtgttt
    14101 gcctgaaaga caacattttc gcctagtcca cctctggctg gtacgaagaa tacattatat
    14161 ggtaataaat gaattgcttc actaacacat gccatcgtgg atccgccagt tacagcaact
    14221 atcgcgtctt cttgtaatat gccttcaagt aattgacctg cttgtctacc taattctgtt
    14281 ttaacagatt gactattatc agcatcacca ggaacaacat gaacttcctt aatttgaaat
    14341 ttattcttaa taccttctga cagacgatta tcatctgcat agatatcaaa gtaacctttc
    14401 aattgcgaaa tcagttgctc accttcagct gtaatttcca ttccggtagg cttaactttt
    14461 atcaaatctt gtttcttaag catatctgtt tcagaacgca gtacacgttc agtcatatcc
    14521 atatgttcgc ttaaacttcg acgtccgaca ggctgatttt ttgagatagt agtaagaata
    14581 gaaaaacgtt tatacatttt atctatgaga tccggtataa gcttttgctg tgcttgcaat
    14641 aagtctttca ctactacctc ctccttatat ttataaatgt aaataaggat atgtcacaac
    14701 cagtggttgc ctttttaagt cccgcgtggg acaaaaatta acctctttac acttgcaata
    14761 gtactattct atttcaaaaa ttgcaagcat aaaacttaaa ttttctaaat aatttgataa
    14821 agcgcttgca atatattttc gatttgttat tcaacaaaac gttttatttt attcgctgta
    14881 gtcaaatgta tacccttata ctgactcgat aatcatttgc ttattatttg agactaggac
    14941 ataaatcaat gctctagaat ccaaaaagtc acattagtag tagttaaccg aacgaaaatg
    15001 cacttgtaac aagctttttt caattaaagt caggggcccc aacatagaga atttcgaaat
    15061 gaaattctac aggcaatgcg agttggggtg gggccccaac atagtgaaat tggctcctcc
    15121 caatttctac aggcaatgca agttggggtg gggccccaac atagagaaat tggatttcca
    15181 atttctacag gcaatggaag ttggggtggg acgacgaaat aaattttgcg aaaatatcat
    15241 ttctgtccca cctcactcaa aataaatttc actatccgta aaataaataa ctaactattt
    15301 aagtatcatc tctcccctaa ttaacaatta taaaaataag aaccaatatt cgagacaacc
    15361 taattaattg tgttgtctgt catactggtt catttttgtt tgactaaata ctacttatca
    15421 ttagcattat tttgtttatt tgttgttgat tggctatttt gttgtggtgc ttgtggtttt
    15481 gcttgctgat ttgattgatt gttttgttga ttattatttg gctttggtgt tggttgttgc
    15541 ggctgtggtt taggctgagc tggttgttta ttatcatcag ttttcttctg attatcatct
    15601 gatttatctt gagatgcttt gtcatcctta tctttcgact catcctttga tgctttatca
    15661 tcattctctt ctttagcagg acgttgcttt gtaggtgctt gttgctgagg tggcacatat
    15721 tgatattgat tctgctgtgg caattgctga tattggtttg cttgtgaatc tagctcattc
    15781 tgctcttttt cctcttgttg ctttttcttt ctctcttttt ctgcattttc tttttgacgc
    15841 ttttcttttt gttcctgctt ttgttcgttc aacatacgtt ctttagcttt attcgaatga
    15901 tctacatatg caaaaattgc aaatactaat cctccgagta atagcaatac cgtcgcaatg
    15961 ctaactactt tcgctgttgt actcattttc ttcttacttc catcttcatt tttattgtag
    16021 tcgtaatttt gattcgacat actctccctc acttcaaact aatttaaata tagaaattat
    16081 cacgcaacca ttaacatttt cacaactaat ttgcttacct agtttctaca aatattaata
    16141 aacatttact ctttttgcct tcttcaactt aaaactgcct tcactattac tcactcgtaa
    16201 atcggattca tttttacaaa gaaatagtag tcttatatat cttaacattt aataactaaa
    16261 tcaaacatat tttgagcctt ttttgaaaat taacatttta acctttttga ttttacaaca
    16321 aattatagct acgtattgaa aattaaagca ttggtttaag tgttgtgtta aaagttttta
    16381 tgtttagatt gttatattac tatgtattct taaatttgtt taaaaaatta atgctttaaa
    16441 ttgatgtata atgggaataa gaaataaata aaaacgaccc gcacgattaa cgtacgggtc
    16501 cactactaaa gggagtcaaa tttttacctt cgtttgtatc atgcagcgtt ttacacatac
    16561 ttttaagaga tgtttattcg ttatcga
//
LOCUS       DAMQYT010000035        16548 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMQYT010000035 DAMQYT010000000
VERSION     DAMQYT010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16548
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.035"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..652
                     /locus_tag="PGA33_000930"
     CDS             <1..652
                     /locus_tag="PGA33_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HDG6472757.1"
                     /translation="VPDKNDKNYRVYKQQDLEKLQKILILKSFDFDIAKIKQYISYDN
                     EQLRKLLSEQISKLDKKISDLQLIRRSVCEFIKGLSLIDTSILNKTLQSQYDKEASIK
                     YGHTKAYQSFIRRKDSLQSQDIRHKLTTIFNKFNHMSLSHYPIQDCSDLVFEWKAFMN
                     TIADFDDETLCCIAKTYEDDTRFKDYFNSYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(910..1563)
                     /gene="nreC"
                     /locus_tag="PGA33_000931"
     CDS             complement(910..1563)
                     /gene="nreC"
                     /locus_tag="PGA33_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HDG6472758.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(1586..2620)
                     /gene="nreB"
                     /locus_tag="PGA33_000932"
     CDS             complement(1586..2620)
                     /gene="nreB"
                     /locus_tag="PGA33_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HDG6472759.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(2644..3096)
                     /gene="nreA"
                     /locus_tag="PGA33_000933"
     CDS             complement(2644..3096)
                     /gene="nreA"
                     /locus_tag="PGA33_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HDG6472760.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(3116..3793)
                     /gene="narI"
                     /locus_tag="PGA33_000934"
     CDS             complement(3116..3793)
                     /gene="narI"
                     /locus_tag="PGA33_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDG6472761.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKPWMDAVGVSEHLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(3786..4361)
                     /gene="narJ"
                     /locus_tag="PGA33_000935"
     CDS             complement(3786..4361)
                     /gene="narJ"
                     /locus_tag="PGA33_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDG6472762.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(4354..5913)
                     /gene="narH"
                     /locus_tag="PGA33_000936"
     CDS             complement(4354..5913)
                     /gene="narH"
                     /locus_tag="PGA33_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDG6472763.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(5903..9592)
                     /locus_tag="PGA33_000937"
     CDS             complement(5903..9592)
                     /locus_tag="PGA33_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDG6472764.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(9901..10878)
                     /gene="cobA"
                     /locus_tag="PGA33_000938"
     CDS             complement(9901..10878)
                     /gene="cobA"
                     /locus_tag="PGA33_000938"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDG6472765.1"
                     /translation="MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVD
                     VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNII
                     SKLQHYSIEAKPGICIIGEVVDYTENTPKSYDPMKQFYVVSGSKHDALMLCEHLYDEG
                     YGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            complement(10869..11183)
                     /gene="nirD"
                     /locus_tag="PGA33_000939"
     CDS             complement(10869..11183)
                     /gene="nirD"
                     /locus_tag="PGA33_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDG6472766.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKGKEVGLFLTESGKIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(11187..13592)
                     /locus_tag="PGA33_000940"
     CDS             complement(11187..13592)
                     /locus_tag="PGA33_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDG6472767.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTLDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARRIFEV
                     EKV"
     gene            complement(13658..14389)
                     /locus_tag="PGA33_000941"
     CDS             complement(13658..14389)
                     /locus_tag="PGA33_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HDG6472768.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGKFTKAHEELQICAQNLQISKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHFTP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            complement(14619..15155)
                     /locus_tag="PGA33_000942"
     CDS             complement(14619..15155)
                     /locus_tag="PGA33_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6472769.1"
                     /translation="MTKTMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            complement(15388..16212)
                     /locus_tag="PGA33_000943"
     CDS             complement(15388..16212)
                     /locus_tag="PGA33_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDG6472770.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
BASE COUNT         4998 a         3415 c         2396 g         5739 t
ORIGIN      
        1 agttccagat aaaaatgata aaaattatcg cgtttataaa cagcaagact tagaaaaatt
       61 acaaaagatt ttaatactca agtcttttga ttttgatatc gctaaaataa aacaatacat
      121 ttcgtatgat aatgaacaat tgcgaaagtt attatcagag caaataagca agttagataa
      181 aaagatttct gacttacaat taattagacg ctctgtatgt gaatttatta aaggactctc
      241 tctaatagat accagcattt taaacaagac actacagtca caatatgata aagaagcatc
      301 tataaaatat ggtcatacga aagcatatca atcatttatt agacgtaaag acagcttaca
      361 atcgcaggat atcagacata aattgacaac tatcttcaat aaatttaatc atatgtcttt
      421 gagtcattat ccaatccaag attgtagtga tctcgtattt gagtggaagg catttatgaa
      481 cactatcgct gattttgatg atgaaacatt atgctgtatt gctaaaacat atgaagatga
      541 tacgcgtttc aaagattact ttaattcata tgataatcaa aatttagcat catacatttc
      601 agaagctgtt aattattttt tgagcaatgt gaataagagc gacaattttt aatcctcata
      661 cagatgctac aacactgaat acaccaacta aaataattgt tcaaagtaga aaactaatca
      721 tttattctct aaatcaataa ctatattaaa agttatacct ttgcaaagcg aattagtata
      781 ggttaccgaa aggagaaagg attaggttcc attcgattta taaaaaaata tatttcgaaa
      841 catacaaaag cgccagaaga taatcatttc ctctcgcgct tttatactga attatattaa
      901 tttcaaactc taaaactcta ataatttctt tttcaatgca tattcaacta gctcaggttt
      961 actctttaag ccaagctttg tcataatatg cgtcttatgt gcttctactg ttttcacaga
     1021 tacaaataat ttctctgcaa tttctttatt cccgtaacct ttggcaataa gtggcaatat
     1081 ttctagttct cgttttgata agattttaaa aggatctgtt gtgtttgcag tgtcctgatt
     1141 tgaattagat acaaattcat tcactaaaga tgttgtaagt ttcatatcta catatgtttc
     1201 acctttatat acagttcgaa tagctaacaa taattgttca tcaggtgcat ttttcaatat
     1261 gtaacctttc gcaccattac gcaacacatg gaacaaatac tcctcatcat caaacattgt
     1321 taatattagt attttagttt caggaaaact gtcagcaatt ttactcgtag cgataagacc
     1381 tgactcacct ggtggcatac ttaaatccat tagtaacaca tcaggtttat attccattac
     1441 tttttggtaa gcttcgacgc catctgcagc cgttgcaaca acttccatat cattttgata
     1501 atttaaaatc atagagaacc ccgtacggac aacagcgtga tcatcggcaa tgactatttt
     1561 caattttatt cccccaatgt atgtttcaaa ttggaatgtt caatgtaaca ttggtaccct
     1621 caccaatttt cgtttcaata ttgacgctac cgctgactaa ctcagctcgc tcattcattc
     1681 catataaacc gagtccagaa cctttaggct tagaacttgg atcaaaacca tttcccgcat
     1741 ctatcacttc tgctaccaaa tggcgcccag tttgacggat acctacattt atttcattta
     1801 catcagcgta tttcaacgca tttaaaatag cttcttgcac tactcgataa acaaccgttt
     1861 caatatcact atcaaagcga gtatttttaa tatttgatgt atatatgatt tttattccat
     1921 aattttcttc aaactgttta aaatatgatt taaaagctgc ttcaaggcct agatcatcca
     1981 aagaagcggg tcttaattca accgacatat tacgtatatc atcaattaat ttagcgacaa
     2041 tatattcaat attttctgcg tcttccaaaa gcttagttgt atcttcttga tattttaata
     2101 atctcaattg aacatctaca ttgagcattt cttgaatcac actatcatgt aactctctag
     2161 aaattcgctt tctttcattt tcttgggctg agattgtttt acgcatcata cgttgttgat
     2221 gcaatttctc ttgctgttca atttgtgatg aaacattttg aagcgtaaat gcatgaattc
     2281 ccctgtcttg atcaatcaac tgatatgttg ctgtaaatgg catcactttt tgatctttcg
     2341 tcttcataaa tacttggaaa ttcgtagctt gtacttgcat cgattctaag aaacagtctt
     2401 tacatgattg tacatcatag gcatttgtat acccttcaca tctgtgacaa atcgcattcg
     2461 ccacagcact gtaattatct tcttcagata atatatcttt agcagcatca ttcattgcaa
     2521 taattttacc gttatcatca gcaaaaacta tcttttcgat tgaatgctca taatattttt
     2581 tcaataaagt atctaactgt atactgtcct cattaatcat gacttacacc ctaattcatc
     2641 tcattattta tcatcattga aaataccaaa cttacgttga atatcatcat tatcaaatat
     2701 ttttggtaaa ggacgaccat ctctttgacc aaataatagt acgccataca cttgattctt
     2761 ataccaaagc ggcactgcta aaactgctgt taatgattcg ctcaataaaa ttggatagtc
     2821 aatcttttct tcaggcccta aagctaaacc aacattggct attaccatac gctttcctgt
     2881 tttcataaca gttccagcta atccacgacc ttttcttaaa ataatcaatt taaatcgatt
     2941 atttttatta cctgaaacat agtgccattt tattggagat gatggtttgt tagattcata
     3001 gaaagcgatt gccgcaaaat cataaccctc ttctttgcgt attttatcta atgtctcttg
     3061 aaatctacga tcttcaatta ttgcttctgg tgtcaaatcc tttcacctct tatgcttaca
     3121 ctttattctt acggtaaata atatatctgc gatttatata tgtcaaaggt acactccaaa
     3181 catgcaccaa acgtgtaaat ggccaacaag ccataatagt gaaacctaac aatatatgca
     3241 ttttaaatgc aatcggcaca ccactcatca atgacgcatc tggttttaac ataaataatt
     3301 gtctaaacca aattgataat gaagttctgt agttaaagtc tggatgttgt atatttgtta
     3361 ctaatgttgc gtaacatccc ataaatacga taagtaataa taagaaattt acaaatatat
     3421 ccgacgctga acttaatctt cgaatacttt tcgtagtaac acgtctcgct gttaataaaa
     3481 acatccctat caaagttatt ataccaaaga tgctaccaat ataaacagcg cctatatgat
     3541 ataaatgctc agacacaccc actgcatcca tccatggttt cggtattaac aatccaacta
     3601 cgtgtccaaa aaacactgga ataataccta agtgaaataa taaacttccc cacatcaacc
     3661 tttttctttc tattaattca ctagatttag ctgtccaaga aaatttatca taacgataac
     3721 gtgcaatatg acctgcgaca aagacaacta aacataaata cggaaatata acccataaaa
     3781 actgattaag catgatgttt cactcctttt ggtgatgtca aacataattt caatgttttt
     3841 ctaagtgctt gaatcacata ggcatatgga ttgttatctt caccaagtgc attcgccatc
     3901 acatatgttc catcctcaat aatcataatg attaattgaa tattctcttc agctcttgga
     3961 tcatttcgcc attctgccac ttgcaaaaat tgaagcatca acggtagata atcagaaagt
     4021 tcattatcta ccatttctag tccaaacatt tcatataata cctttaattt agctaacatt
     4081 tgcccacgtt ctttttgcgt atcaaatttg ttatacgtca tatataatgg tgcttttttc
     4141 gtaaaatcaa atgtatctgt ataaatcgct ttgatttctg ataatgaaaa tgtcatcatt
     4201 acatttctaa atgctactaa atcttcatat gctggatgat ctttcgaaat tgtttcttca
     4261 aaagtttttg gatgaaaagt taatttttct ggaaaacata actgttgtgc catatatcca
     4321 aaactttctt gatatttttt aaaattatcg aaattaatca cggaaaatcc ctccatagaa
     4381 attctcatta taaatttctt gaccagtttt ccctgaacct actgcaacgc cacagccttc
     4441 acagttatct ccaaaatgct cgccgccgta attgtatcct gtactacctt gtgcgtgata
     4501 cgtatctaaa taggtttctt tgtgtgatgt tggaataaca aatcgatctt catatttggc
     4561 tagtcctaat aaacgataca tgtctttagt ttggcgctcg gttataccta atcgctctaa
     4621 tcgagacgtg tcaaatggct gttgagtaac ttgagatctc atataacttc tcatcattgc
     4681 catacgttgt agggctcctt ttactggctc tgtatctcct gcagtgaaaa tattagctaa
     4741 gtattcaata ggtaaacgca tttcttcaat ggctgggaaa atcgcatctg gattttgagt
     4801 tgtattttta ccttcaaaat agctcataat tgggctaagt ggtgggcaat accaaaccat
     4861 cggcatcgtt ctaaattcag gatgtaacgg aaatgcaagt ttatattcaa ttgctaactt
     4921 ataaattgga gagttttgtg cagcttcaat ccaatcgtaa ccaataccat ctttttcagc
     4981 ttgagcaatg acttcttcgt caaatgggtt taagaatata tctaattgtt tttcatataa
     5041 atctttctcg tctactgctg aagctgcttc atgaactcga tctgcatcat ataataaaac
     5101 acctaagtaa cgcatacgtc ctgtacaagt ttcagagcat accgtaggca tacccgcctc
     5161 gattctcggg aaacagaaag tacacttttc agctttgttc gttttccaat tgaagtaaac
     5221 tttcttatat ggacaacctg tcatacagta acgccatcca cgacatgcgt cttggtcaac
     5281 taatacaatg ccatcttcat cacgtttata catagcacct gaaggacacg atgcaacgca
     5341 acttggattc aagcaatgtt cacataaacg tggtaaatac atcataaaag tttcgtcaaa
     5401 ttggaattta atatcttctt ctattttttg gatgttagga tcttttggac ctgtaacatg
     5461 accacctgct aagtcatctt cccagttagg tccccattca atttcaatgt tatcccccgt
     5521 aatttctgaa tacgctctag caactggcga atgcttccct gatttcgcag ttgttaaatg
     5581 ttcataatta tagttccatg gctcataata atctttaatt aatggcatat ctgggttata
     5641 aaaaatttta cctaaagcaa tttttgaaat tctacttcca gattttaatt caagtttccc
     5701 tttacgattt agtacccaac cacctttgta gtgttcttgg tcttcccaac gtttcggata
     5761 ccctacacct ggcttcgttt ctacgttgtt gaaccacatg tactcagcac ctggacgatt
     5821 tgtccaagtg tttttacatg tcacactaca cgtatggcat cctatgcatt tatctaaatt
     5881 taataccatc gcaacttgcg ctttaatctt caagccaatt aacctccttc atctttctaa
     5941 ctgctacata taaatccctt tggttcccaa ttggtccata ataattaaag tgataactaa
     6001 tttgtgcgta tcctccgact agttgtgttg gtttcaaatg gattctagtc ggcgcgttgt
     6061 gtgaaccacc acgtgtatct gtaatttctg acccaggcgt ttgaatatgt ttatcttgtg
     6121 catgatacat aaacattgta cctttaggca tacgatgcga aataactgct cttgccgtta
     6181 caacaccatt acggttatac acttctagcc aatcattatc ttggatatcg tgtttttcag
     6241 catcttcatt tgatatccaa accgttggac cacctctaaa tagtgtcaac atatgcttat
     6301 tatcttgata cattgagtgt atattccatt ttccatgagg cgttaaataa cgcagtacca
     6361 aagcatctgt accaccttta attttcttat ctctattccc aaataccatt ggcggcaatg
     6421 tcggtttata tactggtaag ctctccccaa attgttggaa aacttcgtga tccacataat
     6481 aactttgacg tcctgttaat gttctaaaag gtactagacg ttctatattc gttgtaaatg
     6541 gtgaatatcg tcgaccttgt ttatttgaac ctgggaatac tgctgtcggt attacttctc
     6601 gtggttgtga agttatattt aaaaacgaaa ttttctcagc agcgcgttcg ctagaaatat
     6661 cttttaacgg cattccagtt tgttcttcga gatcttcata tgatttttgt gataatttac
     6721 cattcgtagc agatgaaata cttagtattg catcagctac attacgtgct gtatcaatac
     6781 gtggacgatt cgctctcaca gaatcatcat ttgtatcact ccacgtacct aacatacttt
     6841 ttaattcttc atattgttca ctgacaccga aacttacacc atgtgctcca actttccctt
     6901 tttcaagtac aggaccaagc gtgacatatt tgtcgtaaat tttagtgtag tcgcgttcta
     6961 caattgcaaa gttaggcatt gtacgtccag gtaccgcttc aatttcaccc ttcgaccaat
     7021 ctttcactac gccgtatggt gttgaaattt cttgctttgt atcatgacta agtggagttg
     7081 tcacaacatc tttaaacgtt ccaggtaaat agtcttttgc catttctgaa aatgcttttg
     7141 ccaacgtttt ataaatatcc cagtctgaac gcgattccca taacggatca atggcaggat
     7201 tgaaaggatg tacatatgga tgcatatccg ttgatgataa atcatgtttt tcataccaag
     7261 tcgctgccgg caaaacaatg tcagaatata acggtgttgc cgtcattctg aagtctaaag
     7321 agaccactaa atctaactta cctgttgttt cttcacgcca cgtaatttct tctggctttt
     7381 catcttcatt tggtgtagct aataaccctg attttgtgcc aagtaaatgc ttcataaagt
     7441 attcttgacc ttttgcagaa cttgaaatta agtttgaacg ccatataaat aatgattttg
     7501 gatgattctt tttcaaatca ggatcttcta ttgcaaattg tgtttgtttt gatttcactt
     7561 catcaattgc acgttgcaaa atcgcttcat ttgaatctat accttcatct ttagcttctt
     7621 ctgcaaacaa caaactattt ttattaaatt gtggatatga tggtaaccaa ccaagtctag
     7681 ctgctaaaac attataatca gctggatgtt gatgctttaa ctcctctgtt ttagctaatg
     7741 gagattttaa acgatctaca tttgactctt catatttcca ttggtctgtt gcaaaataga
     7801 accaacttgt tccgttttgc aaacgtggtg gtccttgcca gtctttcgca aatgcgacag
     7861 tactccatcc ttcaatcgga cgacattttt cttgtcccac atagtgagcc caaccgccac
     7921 cgttcacacc ttgacagcca cataacataa ctaagtttaa gattgaacga taaatcgtat
     7981 ctgagttaaa ccaatggtta atacccgcac ccatgataat cattgaacgc ccttcagtat
     8041 cgatagcgtt ttgcgcaaat tctttcgcta cttgaatgac aacactttgt tttacgcctg
     8101 aaatggcttc ttgccaagca ggtgtatatt ttgattctgc atcgtcgtat ccttttgatt
     8161 ctaatttatg atcaaaacga cgcacgccat attgacttgc cattaagtca aaaattgtag
     8221 caatacggac tttgtcacca tttgctaaag tgacttgtcg agttggaatt ggacgattga
     8281 atatcccatc tccatcacta tcaaagtatg ggaattgaat tgtttctaat tcgtatccac
     8341 cttctgtcat tgataatgta gggttaattt tagaaccatc ttctgtttct agttttaagt
     8401 tccacttctt accttcttcc caacgttgac ccattgtgcc attaggtact actaaactat
     8461 cgctgattgc atcatgaata actggcttcc attcgccttg ctctgttgtt tgacctaagt
     8521 cactcgctct taaaaatcga cccgctttat atccattttc atcttcatcc agcatgataa
     8581 gaaacggcat atctgtatat tgtttagcgt aatttataaa gcgttcatta ggttgattaa
     8641 cataatgttc ttgtaaaata acatgcgtca ttgcttgtgc aattgcagca tctgaaccag
     8701 gattcggtgc tagccagtta tctgcaaatt tcacattttc tgcgtaatct ggtgctactg
     8761 aaatgacttt tgtaccttta tagcggactt cagtcataaa atgtgcatcc ggagtacgtg
     8821 ttaaaggtac attagagccc cacataataa tgtatgatgc gttataccag tcacttgatt
     8881 caggcacatc tgtttgctct ccccaaattt gtggagaggc aggtggtaaa tctgcatacc
     8941 agtcataaaa actaagcatt tcaccaccaa gcaaattgat gaatcgagca cctgctgcat
     9001 aactaatcat tgacatcgct ggaataggtg taaatcctgc gattcgatct ggaccatatt
     9061 tttttattgt atacagtaat tgtgctgcga ttatctctgt aacgtctttc caatttgaac
     9121 gcacgtgccc tcccatacct cgggcttgct tatattgttt ggctttgtct tcattttcaa
     9181 caatagacgc ccatgcagca acgcgattac cattgttttc ttctaatgct tcagtccata
     9241 aatcccagag ttttccacga atatatggat atttgattcg aagcggactg tattcatacc
     9301 aagagaatga cgcacctcgt ggacatcctc tcggttcata ttcaggcata tccggaccac
     9361 aacttggata gtcagtttgt tgattttccc aggtaatcac accatttttc acaaatactt
     9421 tccaagaaca tgagcctgta cagttaacac catgtgttgt tcttacttct ttatcgtggc
     9481 tccaacgttc tctgtacatt ttttcccatt ctctactttt actttctagg atcgaccaat
     9541 tcccattaaa tttttctgtt ggcttaaaga aattcaatcc aaattttccc atatttatat
     9601 cctcctacgt ataaaaatac gatgtgtaga tgtcgtgttt ttaaatactt taaaatgccc
     9661 aagactattg ctttaattag attgtacatt ttttcacaaa cataaaatat tagggaatca
     9721 cctaattact taaggaattt ccctatcaat aacgggattt cattgaaata atacacaatc
     9781 atgtatggtc atgcttattg ccaatctaaa tcgttcaaat ttggcacaac gacaaataag
     9841 gcttcaacac gaatatattc tctcggttga aaccttactt attcatttat tttttataaa
     9901 ttagtgacat aacactgtat tagcatctgc acgatcggtt gaaatatatg ttacattttc
     9961 ttgctgctta ataaatgcat catagtaatc atattgcgac gaatgatatg tgccattcga
    10021 tgtatcattt gggtttagca aacagccata accttcgtca tataaatgtt cacagagcat
    10081 aagggcgtca tgtttagaac cacttactac ataaaattgc ttcataggat catatgattt
    10141 aggagtgttt tcagtataat caacaacttc ccctataata catatacctg gtttcgcctc
    10201 aattgaatag tgttgcaatt ttgaaataat attacttaaa cgccccttaa caacaaactc
    10261 gttaaaacac gatgcttgaa agacaatcgc tatcgggtaa tcaatatctg tgtattgttg
    10321 tatctgtgtg ataattttcc ctaaacgttt tacccccata taaattgcta acgtgccacc
    10381 attcactaag gaattgacat ccacttcatt ttcttctgaa tctttaaagt gacctgtaga
    10441 aaatgtcaca cttttagcaa ctgtacgcat tgtcaaacct gtctgcatag tagcaactgc
    10501 tgcgctcgct gatgtcaccc ctggtacaat ttcaaacgca atatgatgtt catttagtgt
    10561 gtcgacttct tcttgcacac gaccaaatat cgctggatcg ccacctttaa gtctaacaac
    10621 cttgttatat cgacgcgctg cttccacgat acagtcattt attttttctt gctgaatatg
    10681 ttttgcatac ggctttttac caacatcgat aatttcagta gtcaaattcg catattgtaa
    10741 aattaacgga ttcactaatc gatcatatag aatgacatcc gcttcacgta ttaaacgctc
    10801 agcctttttc gtcaaataat tcggattacc tggacccgca cctatcaagt aaaccttgcc
    10861 atattcctct acagacatat atatacgttc ccgtctgtaa cttctacctc ataaacatct
    10921 acacaacctt catcaggttc ttgaacaata cctgtattta aatcaatttt ttgatcgtgg
    10981 agcgggcaaa atacatattc cccactcact gtcccttcag acaatggtcc ttgtttgtgt
    11041 ggacagatat tgtgaatcgc atgaattttg ccactttctg ttaaaaacaa ccctacctct
    11101 ttgcctttga caataacctt ttttccaatt aggggtgtta attcatctat agttgtcact
    11161 ttaatttttt cttttgtttc catgtattac accttctcca cttcaaaaat tctacgtgct
    11221 tgcgcattgc tagttattgc ttcccaaggt tcagcttcga ctgctttttt agcatccata
    11281 atgcgttcaa atagttcatt ttgtctttct gggtcaagta agacttcttt tacattttca
    11341 aatccaagtc ttcttaacca tggcgctgtt ctttcagcat atatacctgt ttcgcgataa
    11401 tattgcatca aagcaccaca taatttgatt acttcatctt ctgtttctac agttgttaaa
    11461 aattcagctt tttcaacttc tgtaccacca ttaccaccga tatagatttg gaatccattt
    11521 tcaactgaga taataccaaa atctttaaca cctgattcaa cacaacttct tgggcagcct
    11581 gatacaccca ttttgaattt atgaggtgta tcgatgtatt caaatgtttt ttctaaacga
    11641 atgccaagtc gtgtcgtgta ttgcgtacca aatcgacaaa actctttacc aacacagctt
    11701 ttaactgagc gtgttttctt accataagct gatgctgaac gcatacctag gtcttcccat
    11761 atatttggta attcttcttt tttaactcca tacaaaccaa cacgttgtga acctgtcact
    11821 ttaactagtg gcacatgata tttcttagcc acttctccta gacgaatcag ttggtctgca
    11881 tctgtaacac ccccacgcat ttgaggtata acagaaaatg taccatcatt ttgaatattc
    11941 gcatggtaac gttcgttagc aaatcttgat tctctttcat cttcatgatc atgtggataa
    12001 accatgttta aataatagtt gattgctggt cgacattttg gacatccacc tttattttta
    12061 aagtttaaaa catgtcgaac ttctttagat gtttttaaac ctttcgctct tatttgcgtt
    12121 actatttgat cgcgtgtcaa atcagtacaa ccacatatac cagcaggttt tgcggcaaca
    12181 aagtcatctc ctaaggtgtg ctgcaatatt tgagcaattt gcggtttaca tttaccacat
    12241 gaattccccg cttttgtttt agccgttact tcttcaactg ttgtaaagcc attttccgta
    12301 atcgcattta ctatagtacc tttatcaaca ccattacaac cacaaattgt ttcatcatca
    12361 gccatatcag caattgatag cgatgcctct tctccacctt tagtaagcaa tgatacaagt
    12421 gtgtaatctt cagtggattc accttttttc atcatgttat aaaagcgtga accatcatcg
    12481 atatcaccat atagtactgc accaactaca ttaccgtctt ttaaaaagat ttttttatag
    12541 ttattatcaa cactattaaa tatttcaata cctttaattt ctgcattttc tacaatttga
    12601 ccagcactat acaagtcaca cccagaaact tttaatgacg taaatgttgt tgatcccttg
    12661 tatccgttcg tttctttatt tgttaaatga tcagctaata ctttaccttg ttcatatagt
    12721 ggtgcaacga gtccataaac tttgccgtta tgttctgcac attcaccaac tgcatataca
    12781 ttgctatcac ttgtttgcat cacatcattg acaacaatac cacgattaac atctagacct
    12841 gattctttgg ctacttctgt gtatggtcgt atacctactg ccataacaac taagtctgcc
    12901 ggaatctcgc gtccatcagc caatttaaca ccctcaacat catcttctcc taagatttca
    12961 gttgtgttgg cttgcatttc aaacttcata ccttgctttt ctagatctgc tttaagcata
    13021 tttccagctt tacggtctag ttgcatttcc atcaaccatt cagctaaatg taacaccgtt
    13081 acttccatac cttgatctaa taaaccacgt gcacactcta aacctagtaa tcctccacca
    13141 attacaattg ctttcttttt agtcttagca atgttcatca tttgttcagt atcatcaagc
    13201 gttctccaac caattacact cggtaatgtc gaacctggaa ctggaatgac gaatgcttta
    13261 gaacctgtcg caaaaatcaa tttatcgtat gatacttcaa taccatttgc agtagtaact
    13321 gattgatttg ctctatctac ttcaattaca ggatcatttg taattaactc gataccatgt
    13381 tcctcatacc actcatatgg attcataatt gtttcttcaa ctgtcatttt attttgtaaa
    13441 atatttgaaa gcatgatgcg gttatagttt ggataaggtt ctttacctat taccgtaata
    13501 tcatataaat cgttggcgcg ctctaatatt tcttcgattg ttcgaatgcc cgccataccg
    13561 ttaccaatca ttactagttt ttgctttgcc ataaaatatg cccctttact ccataatatt
    13621 tatttcaaaa aaaggtatta atttttcgtt agtgctttta tattttcatt ggaatcatta
    13681 agctttctaa tctatcgtta atgatttgct ttaaaattgg gtcgaagtta attgaaggtg
    13741 tgaagtgtat atctgtatta ataaccatgt cattcatttg ctgcttcact ttgttaacaa
    13801 gtcttccgtc atataaaaat aatggtacga caatcaattt ttgataccgt ttcgagatgc
    13861 tttctaaatc atgtgtaaaa ctaatctctc catatagcgt tctcgcatat gtcggcttgc
    13921 taatttgcaa attttgagcg catatttgta actcttcgtg tgccttagta aactttccat
    13981 taatattgcc gtgtgcaaca accataactc caacttgttg ttcgtcacct gctaatgcgt
    14041 cacaaatacg ttgttcaatt aatcgtctca ttaaaggatg tgtgccaagt ggctcgctta
    14101 cttctacctt tatgtctgga taccgtcgtt tcatttcatg aacgatattc ggtatatcct
    14161 tgagataatg cattgcacta aagattagca atggtacaat tttaaaatgg tcaaccccac
    14221 tttgaatcaa cgtcgtcatt accgtctcta aatcctgatg ctcactttct aaaaacgcaa
    14281 tatcatagtg atgtatatca tcttttacta attcagaaat aaatgcttct aacgcttgat
    14341 tctgtcgtcc gtgcctcatg ccatgtgcaa caatgatatt cccattcaca tttaccaacc
    14401 ctttcacacg tattgtatac caaatcattt tgtttttgtg aaaagaatca cattataatg
    14461 taaaatcagg gaattccctg atgcctgtag tcatgcatat tccttataca ttttcccttt
    14521 ttgttaaatc aaaaaaagcg accgatatat gaatccctac tcaacattta tttgagcaag
    14581 cattaatata tcggtcgctt gtagtgtata ttattatctt aaaatggtgg ttggcctaat
    14641 attgtttcgt caaagcgctc gggtatcaat actttgcgca tgatcacacc taaatcgcca
    14701 tcatcatttt catgttcgct gtatatttca taacctcttt tttcataaat tttaagtaac
    14761 cacggatgca atcttgcaga tgtacctaaa gtaactgccg ctgactttaa cgtatctcgc
    14821 aaaaatgctt cttcaacata agtaagtaat tggctaccat agcctttccc ttcatactca
    14881 ggatttgtcg caaaccacca gacaaaagga taacccgaaa tacttttcac acttccccaa
    14941 ggatatctaa ccgtaatcgt agatataatt tcatcatcaa ttgtcatgac aaatgtagta
    15001 tttttatcta tattttcttt aacagcatct aaattagcat taaccgaagg ccaatcaata
    15061 cctagttctc ttagaggcgt aaatgcttca tgcatgagtt gttgcaattt ttctgcatct
    15121 tgttcacttg cgagtcgaat catcgttttt gtcatattaa tccccactct tttttaaatg
    15181 atttaaccat attttatttt taaaataaat atccatcaaa gtgtatcaat aaatttatca
    15241 catgtcagaa agtatgcttc atctgaatac accaatactc tcatgaaact tattaaaaat
    15301 tactctctca acgtaaaaaa ccattcaaat tcatgaatgg tttggaagaa tgattcattg
    15361 ttacgctatt taatcactac atcttaatta ttgttgctct aaacgattac gcttaccatt
    15421 taagaaagca taaacgagac ctacaaaaat accgccaccg acaaagttac ctaagaaagc
    15481 aaaaacgata ttttttaaaa cgtgtaacca tgaaactgca tcaaggttaa agaataccat
    15541 acctgcatat agacctgcat tgaacacaac gtgctcatat cccatgtata caaagaccac
    15601 gacaccacaa gctatgaaga atgcctttgt taagccgcct ttgaattgca tagagatgaa
    15661 aataccaata ttaataaaga agttacagaa aatacctttt gtaaaaatat tcaaccatgt
    15721 tgaatcaaca gtctttttct gaactaaagc tgttaaagct tgtgtcattt caggtgtcat
    15781 gacatgcgca aatttcatta agaaaaataa cacaaaccca cctaaaatat ttcctaagaa
    15841 acaatataat aaaatccaag tcatcttctt aacagaaacg actttataat accagcctac
    15901 tgtaaagtac atgaagttac ttgttaataa ttcagagtta gttaatacaa ctaaaatcaa
    15961 acctaaacta aacgcaatgg ctcccattaa attgataagt ccatctacgt gagtactcgc
    16021 aaactgcgtc tttattccga acataaatac agtaacgatt gagagtaaaa atccggccat
    16081 cattgcttta agcatatatc ggccaggtgt ttgctgtacc attacttgct tcatttgtac
    16141 tgaagatgat attgaatcaa caattgcgcc tgttgaatat gtatcttcaa ctgttttctt
    16201 attttctatc acagtacttc ccccatttac tttgatcaaa taaaatttac tttctaatta
    16261 tattagtaaa tcaatgtaat tgatagatta aatttcagaa aaatcttttt attctctcga
    16321 aaatgtagtt ttctttcata aaaacagcga gataaatcgc gtgattggct aatatcttta
    16381 tgatttaaga tattacgacg ctcattttat ctcgctttat gtaaagtatt acttattctt
    16441 ttttaaattt gaaaagtaat gtaaaccata tgcaaataca ctgtaaccaa ttgcagtacg
    16501 taaaataaac cttataactt tattaggtac acattttgac gttactga
//
LOCUS       DAMQYT010000036        16426 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMQYT010000036 DAMQYT010000000
VERSION     DAMQYT010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16426
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.036"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            7..660
                     /locus_tag="PGA33_000944"
     CDS             7..660
                     /locus_tag="PGA33_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDG6472771.1"
                     /translation="MELKVDDFVKNIKRPYLTVLGVFVVAVSLFFDAIMFFYAKLYDK
                     LPMYLLVFMAFTAVILIMMYIQEKNENYKVEKRYVVRYLTLNVIVGYTLPLLFVSIYV
                     FGVVGFGFDVFNYCLGIILMLFISWLGLFLFYKNEFDSENPNPAVNAIAIIIKLFAFG
                     GIFYISLIVPVTQQEEIFIGLSIFINIIVDALLVRSYFNYALYKSIKKDIENEGKTM"
     gene            694..1281
                     /locus_tag="PGA33_000945"
     CDS             694..1281
                     /locus_tag="PGA33_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HDG6472772.1"
                     /translation="MIIFILITIFAIYYIAMIASLFKSEGFSIIGLILDVVIMGTLIF
                     YYFIGARFVDHDLSNFLMFMDTGSYIFMYFAIKCLWVKPKVVNYLIAKELGESKEVIE
                     EQELDLQTSKIRGIYFFIISVVMLIITKLRMQPELQADAISMNPVFIFVGVIIILIWL
                     VLDIYRKKKYGIFLFKTIVPLVVTTWIIIATIILS"
     gene            complement(1424..2317)
                     /locus_tag="PGA33_000946"
     CDS             complement(1424..2317)
                     /locus_tag="PGA33_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDG6472773.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
     gene            2565..2858
                     /gene="esxA"
                     /locus_tag="PGA33_000947"
     CDS             2565..2858
                     /gene="esxA"
                     /locus_tag="PGA33_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HDG6472774.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            2942..5971
                     /gene="esaA"
                     /locus_tag="PGA33_000948"
     CDS             2942..5971
                     /gene="esaA"
                     /locus_tag="PGA33_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HDG6472775.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKTLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKSIADSVSGQLNQV
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLVLVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            5971..6429
                     /gene="essA"
                     /locus_tag="PGA33_000949"
     CDS             5971..6429
                     /gene="essA"
                     /locus_tag="PGA33_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HDG6472776.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            6401..6643
                     /gene="esaB"
                     /locus_tag="PGA33_000950"
     CDS             6401..6643
                     /gene="esaB"
                     /locus_tag="PGA33_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HDG6472777.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            6656..7990
                     /gene="essB"
                     /locus_tag="PGA33_000951"
     CDS             6656..7990
                     /gene="essB"
                     /locus_tag="PGA33_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HDG6472778.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            8012..12451
                     /gene="essC"
                     /locus_tag="PGA33_000952"
     CDS             8012..12451
                     /gene="essC"
                     /locus_tag="PGA33_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HDG6472779.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGIATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRIFNDEIDRRK
                     KILSQYRVTSISEYRKLTGETIPHVFILIDNFDAVKDSPFQEVFENMMIKMTREGLAL
                     DMQVTLTASRANAMKTPMYINMKTRIAMFLYDKSEVSNVVGQQKFAVKDVVGRALLSS
                     DDNVSFHIGQPFKHDETKSYNDQINDEVSAMTEFYKGETPNDIPMMPDEIKYEDYRES
                     LNLPDIVANGALPIGLDYEGVTLQKIKLTEPAMISSENPREIAHIAEIMMKEIDILNE
                     KYAICIADSSGEFKAYRHQVANFAEEREDIKAIHQLMIEDLKQREMDGPFEKDSLYII
                     NDFKTFIDCTYIPEDDVKKLITKGPELGLNILFVGIHKELIDAYDKQIDVARKMINQF
                     SIGIRISDQQFFKFRFIQREPVIKENEAYMVANQAYQKIRWFK"
     gene            12481..12873
                     /gene="esaC"
                     /locus_tag="PGA33_000953"
     CDS             12481..12873
                     /gene="esaC"
                     /locus_tag="PGA33_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HDG6472780.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSFALDDIESTAKDVQTAKSSANKDSVTVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKEIAGEKASEYFNKAMN"
     gene            12889..13203
                     /gene="esxB"
                     /locus_tag="PGA33_000954"
     CDS             12889..13203
                     /gene="esxB"
                     /locus_tag="PGA33_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HDG6472781.1"
                     /translation="MGGYKGIKADGGKVDQAKQLAAKTAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPMADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            13203..13877
                     /locus_tag="PGA33_000955"
     CDS             13203..13877
                     /locus_tag="PGA33_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HDG6472782.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKAALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDETLENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLFIRYPLKDKVLNVHIGVINTFIRELFGFDTDENH
                     INKAEE"
     gene            13877..14194
                     /locus_tag="PGA33_000956"
     CDS             13877..14194
                     /locus_tag="PGA33_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472783.1"
                     /translation="MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYY
                     SSEKAAHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            14204..16048
                     /locus_tag="PGA33_000957"
     CDS             14204..16048
                     /locus_tag="PGA33_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease YeeF family protein"
                     /protein_id="HDG6472784.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEELEEQKKKEEAIEIAALA
                     GMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNIKRAVTPQNHVTHGPKDSMVRSEGKHSISSHEMNSSKYVES
                     PNYTKVEFGEHYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGDRN
                     SHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKFINRPFKEKGHWYNLEKE
                     WQEFLNSGKEVKNIKMEVKYSGNSQRPTIFKVEYEINGERNIRRILNK"
     gene            16059..>16426
                     /locus_tag="PGA33_000958"
     CDS             16059..>16426
                     /locus_tag="PGA33_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDG6472785.1"
                     /translation="MTFEEKLSKIYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPGSDDLNYYTNIPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEDLEPWTSCEF
                     DFTREGELKVSFDYIDWINS"
BASE COUNT         6185 a         2238 c         3107 g         4896 t
ORIGIN      
        1 gaatttatgg aattgaaagt cgatgatttt gtaaagaata taaaaagacc atacttgact
       61 gtattgggag tatttgtagt tgcagtttca ttattttttg acgcgattat gtttttctac
      121 gctaaactat atgataaatt gcctatgtat ttactggtgt ttatggcctt tacagctgta
      181 attttgatta tgatgtacat acaagagaaa aatgaaaatt acaaagttga aaaaagatat
      241 gtggttagat atctcacact taacgttatt gtgggatata ctttgccatt gctttttgta
      301 tctatttacg tttttggtgt agtcggtttt ggatttgatg ttttcaatta ttgtctaggt
      361 attatcttga tgttatttat ttcttggtta ggtttatttt tattttataa aaacgaattt
      421 gatagtgaaa atcctaatcc tgcagttaat gccatagcaa ttattataaa attatttgct
      481 tttggaggaa tattttatat aagtctaata gttcctgtaa cacagcaaga agagattttt
      541 atagggttaa gtatattcat caatataatt gttgatgctc ttcttgttag gtcatatttt
      601 aattacgcgt tatataagag tattaagaaa gatatcgaaa atgaaggtaa gacaatgtaa
      661 aggaaagaca taaaaaataa taggagtcta accatgataa tttttatttt aataacaata
      721 tttgctattt attatatagc tatgattgct agtttgttta aaagtgaagg tttttcaata
      781 ataggtttaa tattagatgt tgttataatg gggacattga ttttttacta ttttataggc
      841 gctcgctttg ttgatcatga tttaagcaac tttttaatgt ttatggatac tggatcatat
      901 atttttatgt actttgcaat taagtgttta tgggtgaaac ctaaggtagt aaattatttg
      961 attgcaaaag aattgggtga atctaaagag gtcattgaag agcaggaatt agatttacag
     1021 acatcaaaga taagaggtat ctactttttc attatttcag tagtgatgtt aattatcact
     1081 aaattaagaa tgcaacctga gttacaggca gatgctatat cgatgaatcc tgtatttatt
     1141 tttgttggtg ttataatcat tttaatttgg ctagtactgg atatatatcg caagaaaaaa
     1201 tacggtatat tcttattcaa gacgatagtg ccactagttg ttacgacttg gattattata
     1261 gctacaatta tactttcgta aacgattttg attaatgggg aatgtagaga acatttcttt
     1321 gtaattttat aatcgaataa catacaaaat aaagcgacca attttgcagt gttacgaatt
     1381 gtaaactgcg aactggtcgc tttttattga tgtcatgatt aatttaatgg atgtaattat
     1441 atgatgaaac ttctgaagca gagatggttc ttgatgaaac gatatattca ccaatccagt
     1501 tcatttctga aattagaata cttccatcaa tattaacttt ttcaacgtag gctacatgac
     1561 caaatggacc atttactgtt tgtaaaattg atcctcgtgt tgggtgtcta tctactttga
     1621 agccattgct tgaagcttgg cctgcccagt ttttagcatc tccccaaaat gtactaatcg
     1681 tgtgtccatc tttggcacgt ttatcaaaga cataccatgt acattgtcca gcagtatata
     1741 agttgttctt acttgtgata agaggctgat caatgatttt accgttacct aatgctaaag
     1801 gtttaccgtc agcggtcttt gctttgtcat taaattcggc gatttgtaat tcgtcataca
     1861 attcgtctaa atcgatgcct gtaataagcc ctttgttatc ttttgaaaaa gcgttattta
     1921 attcatcgtc attgtcttcg acattcggta ttgctggtgt caaaggattg cttggtgacg
     1981 tttgaggcgg tgtgtgtgaa tcaattgcgt cattaatgtg cgtatactga ccacttaatg
     2041 aagaatggta ctgattgttg ttaagatcac gttgatttgc gtgttgatga ttgtcgtttg
     2101 tacgtgactg gttttgatga ttgttgtttg gcgtgttgtt tttgtcatat gtataagtat
     2161 acgcgccggt gtctttattc actttgaact gtgcgtttgg gtgtgctttc tttgcttctt
     2221 ctaatgtttt gctatcattc gtatatgctt gagccgagtt aggcgacata ctaaataaag
     2281 taagagttgt catcgtcagt aaaattgttt tcttcataat aaccatttaa tcctttatgt
     2341 atttaattta attttagtat acacatttat attacaaaaa tgaatggtta attaaaaata
     2401 tatgggtata ttcaatatat ttatttaaaa aaagctaaaa atacttaaaa aactatatac
     2461 atatactaat aatttatata ttatttgagt aaggagcact ttttcaaaaa atagtgtccc
     2521 taaaaagttt tgataaactt aaaatattca ggaggtttct agttatggca atgattaaga
     2581 tgagtccaga ggaaatcaga gcaaaatcgc aatcttacgg gcaaggttca gaccaaatcc
     2641 gtcaaatttt atctgattta acacgtgcac aaggtgaaat tgcagcgaac tgggaaggtc
     2701 aagctttcag ccgtttcgaa gagcaattcc aacaacttag tcctaaagta gaaaaatttg
     2761 cacaattatt agaagaaatt aaacaacaat tgaatagcac tgctgatgcc gttcaagaac
     2821 aagaccaaca actttctaat aatttcggtt tgcaataagc attctgaaat tggcaaagtc
     2881 acattttcta atgtggcttt gcttatcatt tttttaagaa aacaactgaa aggaaataag
     2941 catgaaaaag aaaaattgga tttatgcatt aattgtcact ttaattatta taattgccat
     3001 agttagtatg atattttttg ttcaaacaaa atatggagat caatcagaaa aaggatccca
     3061 aagtgtaagt aataaaaata ataaaataca tatcgcaatt gttaacgagg atcaaccaac
     3121 gacatataac ggtaaaaaag ttgagctggg tcaagcattt attaaaaggt tagcaaatga
     3181 gaaaaactat aaatttgaaa cagtaacaag aaacgttgct gagtctggtt tgaaaaatgg
     3241 tggataccaa gtcatgattg ttatcccaga aaacttttca aaattggcaa tgcaattaga
     3301 cgctaaaaca ccatcgaaaa tatcgctaca gtataaaaca gctgtaggac aaaaagaaga
     3361 agtagctaaa aacacagaaa aagttgtaag taatgtactt aacgacttta acaaaaactt
     3421 agtcgaaatt tatttaacaa gcatcattga taatttacat aatgcacaaa aaaatgttgg
     3481 cgctattatg acgcgtgaac atggtgtgaa tagtaaattc tcgaattact tattaaatcc
     3541 aattaacgac ttcccggaat tatttacaga tacgcttgta aattcaattt ctgcaaacaa
     3601 agacattaca aaatggttcc aaacatacaa taaatcatta ttgagtgcga attcagatac
     3661 gttcagagtg aacacagatt ataatgtttc gactttaatt gaaaaacaaa attcattatt
     3721 tgacgagcac aatacagcga tggataaaat gttacaagat tataaatcgc aaaaagatag
     3781 cgtggaactt gataactata tcaatgcatt aaaacagatg gacagccaaa ttgatcaaca
     3841 atcaagtatg caagatacag gtaaagaaga atataaacaa actgttaaag aaaacttaga
     3901 taaattaaga gaaatcattc aatcacaaga gtcaccattt tcaaaaggta tgattgaaga
     3961 ctatcgtaag caattaacag aatcactgca agatgagctt gcaaataaca aagacttaca
     4021 agatgcgcta aatagcatta aaatgaacaa tgctcaattc gctgaaaact tagagaaaca
     4081 acttcatgat gatattgtca aagaacctga tacagataca acatttatct ataacatgtc
     4141 taaacaagac tttatagctg caggtttaaa tgaggatgaa gctaataaat acgaagcaat
     4201 tgtcaaagaa gcaaaacgtt ataaaaatga atataatttg aaaaaaacgt tagcagaaca
     4261 cattaattta acagattacg ataaccaagt tgcgcaagac acaagtagtt tgattaatga
     4321 tggtgtcaaa gtgcaacgta ctgaaacgat taaaagtaat gatattaatc aattaactgt
     4381 tgcaacagat cctcatttta attttgaagg cgacattaaa attaatggta aaaaatatga
     4441 cattaaggat caaagtgttc aactcgatac atctaacaag gaatataaag ttgaagtcaa
     4501 tggcgttgct aaattgaaaa aggatgctga gaaagatttc ttaaaagata aaacaatgca
     4561 tttacaattg ttatttggac aagcaaatcg tcaagatgaa ccaaatgata agaaagcaac
     4621 gagtgttgtg gatgtaacat tgaatcataa ccttgatggt cgcttatcga aagatgcatt
     4681 aagccagcaa ttgagtgcat tatctaggtt tgatgcgcat tataaaatgt acacagatac
     4741 aaaaggcaga gaagataaac cattcgacaa caaacgttta attgatatga tggttgacca
     4801 agttatcaat gacatggaaa gtttcaaaga cgataaagta gctgtgttac atcaaattga
     4861 ttcaatggaa gaaaactcag acaaactgat tgatgacatt ttaaataaca aaaagaatac
     4921 aacaaaaaat aaagaagata tttccaagct gattgatcag ttagaaaacg ttaaaaagac
     4981 ttttgctgaa gagccacaag aaccaaaaat tgataaaggc aaaaatgatg aatttaatac
     5041 gatgtcttca aatttagata aagaaattag tagaatttct gaaaagagta cgcaattgct
     5101 atcagataca caagaatcaa aatcaattgc agattctgtt agtggccaat taaatcaagt
     5161 cgacaataat gtgaataagc tacatgcgac aggtcgagca ttaggcgtaa gagctaacga
     5221 tttgaatcgt caaatggcta aaaacgataa agataatgag ttgttcgcta aagaatttaa
     5281 aaaagtatta caaaattcta aagatggcga cagacaaaac caagcattaa aagcatttat
     5341 gagtaatccg gttcaaaaga aaaacttaga aaatgtttta gctaataatg gtaatacaga
     5401 cgtgatttca ccgacattat tcgtattatt gatgtattta ctatcaatga ttacagcata
     5461 tattttctat agttatgaac gtgccaaagg acaaatgaat ttcattaaag atgattatag
     5521 tagtaaaaac catctttgga ataatgtcat tacgtcaggt gttattggta caactggttt
     5581 ggtagaaggg ttaattgtcg gtttaattgc aatgaataag ttccatgtat tagctggcta
     5641 tagagcgaaa ttcatcttaa tggtgatttt aactatgatg gtcttcgtac ttattaatac
     5701 gtatttacta agacaggtaa aatctatcgg tatgttctta atgattgctg cattgggtct
     5761 atactttgta gctatgaata atttgaaagc agctggacaa ggtgtgacta ataaaatttc
     5821 accattgtct tatatcgata acatgttctt caattattta aatgcagagc atcctatagg
     5881 cttggtgcta gtaatattaa cagtacttgt gattattggc tttgtactga acatgtttat
     5941 aaaacacttt aagaaagaga gattaatcta atgttgatga atagcgtgat tgctttaact
     6001 tttttaacag catctagcaa taatggcgga cttaatattg atgtgcaaca agaagaggaa
     6061 aagcgaatca ataatgattt aaatcaatat gatacaacgc tatttaataa agacagcaaa
     6121 gcggttaatg atgcgattgc taagcagaaa aaagaacgac aacaacaaat aaaaaatgat
     6181 atgtttcaaa atcaagcgag tcactcgact cgcttgaatg aaactaaaaa agtgttattt
     6241 tccaaatcta acttagaaaa gacttcggag agtgataaaa gcccctatat tcaaaacaag
     6301 caggagaaaa aaatattccc gtacattttg atgtctgtag gggctttttt gactttagga
     6361 tttgtcattt tttcaattca taaagggaga cgaacgaaaa atgaatcagc acgtaaaagt
     6421 aacatttgat tttactaatt ataattacgg cacatatgac ttagcagtac cagcatattt
     6481 accgataaaa aacttaatag ctttagtatt ggatagtttg gacatttcaa tatttgatgt
     6541 caatacacaa attaaagtga tgacgaaagg tcaattactt gttgaaaatg atcgactcat
     6601 tgattatcaa atcgctgatg gagatatttt gaagttacta taggaggaaa aatagatggt
     6661 taaaaatcat aaccctaaaa atgaaatgca agatatgtta acgcctttag atgctgaaga
     6721 agcagctaaa acaaaattac gcttagatat gagagagatt cctaagtctt caattaaacc
     6781 agaacatttt catttaatgt acttattaga acaacattct ccatatttta tagatgctga
     6841 attaactgaa ctacgtgaca gtttccaaat acattatgac attaatgaca atcatacacc
     6901 ttttgataat attaaatcat ttactaaaaa tgaaaaatta cgttacttac tcaatatcaa
     6961 aaatttagaa gaagtaaatc gtacacgcta cacatttgtg ttggcaccag atgaattatt
     7021 tttcacaaga gatggattac ccattgctaa aacaagaggg ttacaaaatg ttgttgatcc
     7081 attacctgtg tcagaagctg aatttttaac aagatataaa gcgctggtta tctgtgcatt
     7141 caatgagaaa caatcatttg atgctttagt tgaaggaaac ttagaactac ataaaggaac
     7201 gccatttgaa actaaagtta ttgaagcggc aacgttagat ttactaacgg catttttaga
     7261 tgaacagtat cagaaacaag aacaagatta tagtcaaaat tatgcatatg tacgcaaagt
     7321 aggacatacc gttttcaaat gggttgctat cggtatgaca acgttaagtg ttttattaat
     7381 tgcattctta gcctttttat atttttcagt aatgaagcat aatgagcgca ttgaaaaagg
     7441 ataccaagca tttgtaaagg atgattatac gcaagtacta aatacgtatg atgatttaga
     7501 tggtaaaaaa ttagataaag aggcacttta catttatgcc aaaagttata tccaaacaaa
     7561 taaacaaggt ttagaaaaag ataagaaaga aaatttactt aataatgtga caccaaattc
     7621 aaacaaagac tacttattat attggatgga attaggacaa ggacatcttg atgaagcgat
     7681 taatattgcc acttatttag atgataacga tattacaaag ttagcgttga ttaataaatt
     7741 aaatgagatt aaaaataacg gagatttatc gaatgataaa cgttctgaag aaacgaaaaa
     7801 gtataacgat aaattgcaag atattttaga caaagaaaaa caagttaaag atgaaaaagc
     7861 gaaatctgaa gaagagaaag caaaagcgaa agatgagaaa ttaaagcaac aagaagagaa
     7921 cgaaaagaaa caaaaagaac aagcacaaaa agataaagaa aaacgccaag aagctgaaag
     7981 aaaaaaatag tataggactg aggcaaagac aatgcataaa ttgattataa aatataacaa
     8041 acaattgaag atgctcaatt tgcgagatgg taagacatat actattagcg aagacgagcg
     8101 tgcagatatt acgttgaaat cgttaggcga agtcattcat ttagaacaaa ataatcaagg
     8161 tacttggcaa gcgaatcata cttctattaa taaggtgctt gttagaaaag gtgaccttga
     8221 tgacattaca ttacagcttt atacagaagc tgattatgca tcatttgcgt atccttcaat
     8281 tcaagatacg atgacaattg gaccaaatgc gtatgatgat atggttattc aaagcttgat
     8341 gaatgccatc attattaaag attttcaatc aatacaagaa tcacaatacg tacgcattgt
     8401 gcacgataaa aatacagatg tgtatattaa ctatgaacta caagagcaac taacgaacaa
     8461 agcttacatt ggtgatcata tttatgttga agggatatgg ctcgaagtac aagctgatgg
     8521 tttaaatgta ttgagtcaga atacagtggc atcgtcatta attcgcttaa cacaagagat
     8581 gccacatgca caggcagatg attacaatac gtaccatcgt tcgccaagga ttattcaccg
     8641 tgaaccgacg gatgatatta agattgaaag accgccacag ccaatacaga agaacaatac
     8701 agtgatatgg cgttccatta taccgccatt agtaatgatt gctttaactg ttgtcatctt
     8761 tttagtgaga ccaattggta tttatatttt aatgatgatt ggtatgagta cagtaacgat
     8821 agtatttggt attacaacgt atttctctga aaagaaaaag tataacaaag atgttgaaaa
     8881 acgagagaaa gattacaaag cttatttgga taataaatct aaagaaatta acaaagcgat
     8941 taaagcacaa cgttttagtt tgaattacca ttatccaacg gttgctgaaa ttaaagatat
     9001 cgttgaaacg aaagcaccaa gaatatatga aaaaacatcg catcatcacg atttcttaca
     9061 ttataagtta ggtattgcga atgtagaaaa gtcattcaaa ttagattacc aagaagaaga
     9121 atttaaccaa cgtcgtgatg aactattcga cgatgctaaa gaattgtatg aattttacac
     9181 agatgtagaa caagcaccat taatcaatga tttaaatcat gggccaattg catatattgg
     9241 tgcacgacat ctcattttag aagaattgga gaaaatgcta attcaattgt caacattcca
     9301 tagttatcat gatttagagt ttctatttgt gacacgtgaa gatgaagttg aaacattgaa
     9361 atgggcacgt tggttgccac atatgacatt gagagggcaa aacattagag gatttgttta
     9421 caatcaacga acgcgtgacc aaattttaac gtcaatttat agcatgatta aagaacgtat
     9481 ccaagctgtg cgtgaacgca gcagaagtaa tgagcaaatt attttcacac cgcaattagt
     9541 gtttgtcatt acagatatgt cattaattat tgatcatgtc attttagaat atgtaaacca
     9601 agatttatca gaatatggta tttcattaat ctttgttgaa gatgtgattg aaagtttgcc
     9661 agagcatgta gataccatta ttgatatcaa gtctcgtact gaaggcgaac tgattacgaa
     9721 agaaaaagaa ttagttcaat tgaaatttac acctgaaaat attgataacg tcgataaaga
     9781 atatatcgcg cgacgtttgg cgaatttgat acacgtcgaa catttgaaaa atgcaattcc
     9841 tgatagtatt acatttttag agatgtataa cgtgaaagaa gtagatcagc ttgatgtggt
     9901 taatcgatgg agacaaaacg aaacatacaa aacgatggca gtacctttag gtgtaagagg
     9961 taaagatgat attttatcat tgaacttaca tgaaaaagca cacgggccac atggtttagt
    10021 tgctggtacc actggttcag ggaaatctga gattatccaa tcatacattt tatctttagc
    10081 tattaatttt caccctcatg aagttgcatt cctattgatt gactataaag gtgggggtat
    10141 ggcgaactta tttaaagatt tagtccattt agttggtacg attacaaact tagatggcga
    10201 tgaagcgatg cgtgccttaa catcaatcaa agccgaattg agaaaacgtc aacgtttatt
    10261 cggagagcat gatgttaacc atattaatca ataccataag ttatttaaag aaggtattgc
    10321 gacagaacca atgccacatt tattcattat ttccgatgag tttgccgaat taaaatcaga
    10381 acaacctgat tttatgaaag aacttgtatc aacggcacgt attggacgtt cgttaggtat
    10441 tcatttaata cttgcgacac aaaaaccatc gggtgttgtt gatgaccaaa tttggtctaa
    10501 ctctaaattt aagttggcat taaaagtaca agatagacaa gacagtaatg aaattttaaa
    10561 aacaccagat gcagcagaca ttacattacc aggtcgtgcg tatttacaag ttggtaataa
    10621 tgaaatttat gaattattcc aatctgcatg gagtggtgca acatatgaca tcgaaggcga
    10681 taaattagaa gttgaagata agacgattta catgattaat gactatggtc aacttcaagc
    10741 aatcaacaaa gacttgagtg gacttgaaga tgaagaaacg aaagaaaatc aaactgagtt
    10801 agaagcggtc atagatcata tcgaatctat tacaacacga ttagaaatcg aagaagttaa
    10861 gcgtccatgg ctaccaccat tgccagaaaa tgtatatcaa gaagatttag tagaaacaga
    10921 tttcagaaaa ttatggtcag atgatgcaaa agaagtggaa ttaacattag gacttaaaga
    10981 cgtaccagaa gaacaatatc aaggaccgat ggtattgcaa ttgaaaaaag ctgggcacat
    11041 cgcgttaatc ggaagtccag gatatggtag aacaacgttc ttacacaaca ttattttcga
    11101 tgttgcaaga caccatcgtc ctgatcaagc acacatgtac ttgttcgatt tcggtaccaa
    11161 tggtttgatg ccagttacag acataccaca tgtcgctgat tactttacag tagatcaaga
    11221 agacaagatt gctaaggcga tacgtatatt taatgatgaa attgatcgtc gtaagaagat
    11281 tttaagtcag tatcgtgtca ctagtatttc tgaatatcga aaattaactg gtgaaacaat
    11341 tccgcatgtc tttattctta ttgataactt tgacgcagta aaagattcac ctttccaaga
    11401 agtttttgaa aatatgatga ttaaaatgac gcgtgaaggg ctagcattag acatgcaagt
    11461 aaccttaact gcttcaagag ctaacgctat gaaaacacca atgtacatta atatgaaaac
    11521 gcgtatcgca atgtttttat atgataaatc agaggtgtcg aacgtagtag gacagcaaaa
    11581 atttgcggtt aaagatgttg tgggtcgagc attgttaagt agtgatgaca acgtatcatt
    11641 ccatattggc caaccattta aacatgatga gaccaaatca tataatgatc aaattaatga
    11701 tgaagtatcg gcgatgacag aattttataa aggtgaaaca ccaaatgata ttcctatgat
    11761 gccagatgaa attaaatatg aagattacag agaatcatta aacttaccag atatagttgc
    11821 aaatggtgct ttaccaattg gattagatta tgaaggtgtt acactacaaa aaattaaatt
    11881 aactgaacca gcaatgattt catcagaaaa tccgagagaa attgcgcata ttgctgaaat
    11941 tatgatgaaa gaaattgaca tattaaatga aaaatatgcg atttgtatcg cagactcaag
    12001 tggagagttt aaagcttata ggcatcaagt ggctaacttt gccgaagaaa gagaagacat
    12061 taaagcgatt catcaactaa tgattgaaga cttaaagcaa agagaaatgg acggcccatt
    12121 tgaaaaagat tcactttata ttatcaatga ttttaaaaca tttattgatt gcacgtatat
    12181 tccggaagat gatgttaaaa agcttattac aaaaggacca gaacttggct tgaacatttt
    12241 atttgtcggc attcataaag aattaataga tgcttatgat aaacagattg atgttgcacg
    12301 taaaatgatt aaccaattta gtataggtat tcgtatttca gaccaacaat tctttaaatt
    12361 tagatttatt caacgagaac ctgttattaa agaaaatgaa gcatatatgg tcgcaaacca
    12421 agcttatcaa aagattagat ggtttaaata gcaatgaatt aaataggagg gaggtatgtt
    12481 atgaatttta atgatattga aacaatggtt aagtcgaaat ttaaagatat taaaaagcat
    12541 gctgaagaga ttgcgcatga aattgaagtt cgttctggat atttaagaaa agctgaacaa
    12601 tataagcgat tagaatttaa tttgagtttt gcactagatg atattgaaag cacagcaaag
    12661 gacgtacaaa ctgcaaaatc tagtgctaat aaggacagtg taactgttaa gggaaaggcg
    12721 cccaatacgt tatatattga aaaaagaaat ttgatgaaac aaaagcttga aatgttgggt
    12781 gaagatatcg ataaaaataa agaatccctc caaaaagcta aggaaattgc tggcgaaaag
    12841 gcaagtgaat attttaataa agcaatgaat taatattgag gtgaagatat gggtggatat
    12901 aaaggtatta aagcagatgg tggcaaggtt gatcaagcga aacaattagc ggcaaaaaca
    12961 gctaaagata ttgaagcatg tcaaaagcaa acgcaacagc tcgctgagta tatcgaaggt
    13021 agtgattggg aaggacagtt cgccaataag gtgaaagatg tgttactcat tatggcaaag
    13081 tttcaagaag aattagtaca accgatggct gaccatcaaa aagcaattga taacttaagt
    13141 caaaatctag cgaaatacga tacattatca attaagcaag ggcttgatag ggtgaaccca
    13201 tgatgaaaga tgttaagcga atagattatt tttcttacga agaattaaca attttaggtg
    13261 gtagtaaatt gcctctcgta aattttgaat tgtttgatcc atcaaatttt gaagaagcta
    13321 aagctgcttt aattgaaaag gaattagtaa cagagaatga caagttaact gatgcaggtt
    13381 ttaaagtggc tacattagtc agagagtata ttagcgccat tgtaaatatt cgaattaatg
    13441 atatgtattt tgcaccattt agctatgaaa aagatgaata tattttgtta agccggttta
    13501 aaaataatgg gtttcaaata cgaattatca ataaagacat tgcatggtgg tcgattgtac
    13561 aatcatatcc tttattgatg agacaagaaa agtccaatga ttgggacttt aaacaaattg
    13621 acgatgaaac attggagaac ttaaataatg aaagtatcga tacgattggg cgtgttttag
    13681 aaattgaaat atacaatcat caaggtgacc ctcaacaaag tttatataac atttatgaac
    13741 aaaatgattt gttattcatt cgatacccat taaaagataa agtgctgaat gttcatattg
    13801 gtgtcattaa tacatttata cgagaattat ttggattcga tactgatgaa aatcatatta
    13861 ataaagcaga ggagtaatga cgttgagtgg aaaaattagt gttaaagctg aaacgattgc
    13921 acatgttgta aaagaattgg aaagcataag tcaaaagtat gatgaaatag ctcaaaactt
    13981 tggaaaaata gcgcaattaa attactacag tagtgaaaaa gctgcacatt ctatggaaaa
    14041 tggctatagt agtgctgcaa cagtcattag tggtctcaaa ggtccattga gtacactcgg
    14101 tggtggcgtc atgaattcag cacaaaagtt ctttgaagca gatgaacatt ggggtacgga
    14161 atttgccaag ctttactata atattgaggg ataggtgcat gacatgacaa aagatattga
    14221 atatctaaca gctgattatg acaatgaaaa gtcatctatc caaagtgtaa tagatgcaat
    14281 agagggtcaa gacttcttag atgtagatac aacaatggat gatgcggtaa gcgatgtcag
    14341 ttctttagac gaagatggcg caatatcatt aacaagtagt gtagtaggtc cacaaggatc
    14401 taaattaatg gggtattatc aaaatgagtt atatgattat gcatctcaat tagattcgaa
    14461 aatgaaagaa attattgaca cgccatttat agaagatata gataaagcat tcaaaggtat
    14521 aacgaatgtt aaattggaaa atatactaat taaaaatggt ggtggacatg gtagagatac
    14581 ctatggggct tctgggaaaa ttgcaaaggg agatgccaag aaaagtgaca gcgatgttta
    14641 tagcatcgat gaaatattaa aatcggatca agaatttgta aaagtaattg atcagcatta
    14701 caaagaaatg aaaaaagaag ataagaaatt atctaaaagt gattttgaaa aaatgatgac
    14761 tcagggcgct tcttgtgatt acatgacagt agctgaagcg gaagagctag aggagcaaaa
    14821 gaaaaaagaa gaagctatag agattgcagc actagctggt atggtagttt tatcttgtat
    14881 taatcctgtt gctggagcag tagctattgg tgcttattcc gcttattcag cagcaaatgc
    14941 agccacagga aaaaatattg taactggaag aaagctatct aaagaagaac gaatcatgga
    15001 aggactttcg cttattccat tgccaggtat gggcttcctc aaaggtgctg ggaaaagttt
    15061 aatgaaatta ggcttcaaag gcggagaaaa atttgcagtt aaaacaggat tgcaaaagac
    15121 aatgcaacaa gcagttagtc gtatttcacc taaaatggga atgatgaaaa acagtgtgtt
    15181 gaatcaatct cgtaactttg ctcaaaatac tcatgttgga caaatgctga gtaacatgcg
    15241 tggtcaagca actcatactg ttcaacaaag tagaaattgg attggacaac aagcacaaaa
    15301 tgtcaaacga atagtgaata atggacttga taaagaaata gcacatccat ttaaacaaca
    15361 acttgcacca gcgggaatgg gtggtataaa atttgctgaa acaactactt tgagaaacat
    15421 gggtcaaaac ataaagcgtg ctgttacacc acaaaatcac gtgacacatg gtccaaaaga
    15481 tagtatggtg agaagtgaag gcaaacatag tataagtagc catgaaatga attcatcaaa
    15541 gtatgttgaa tcaccaaact acaccaaggt tgaattcgga gaacactatg caagacttag
    15601 acctaagaaa ctaaaggcga atattgaata cacaacacct actggtcaca tatatcgaac
    15661 cgatcataaa ggtcgcataa aagaagttta tgtagacaat ctctctctaa aagatgggga
    15721 tcgtaatagc catgcacaaa gaactgtggg gggagaggat agattaccag acgatgatgg
    15781 aggtcactta atcgctagaa tgtttggtgg ttcaaaagac attgataacc ttgtggcaca
    15841 aagtaaattt atcaaccgtc catttaagga gaaaggtcat tggtataatc ttgagaaaga
    15901 gtggcaagag ttcttaaact ctgggaaaga ggtgaaaaat attaaaatgg aagtaaaata
    15961 tagcggtaat agtcaaagac cgactatatt taaagttgaa tatgaaatta atggtgaaag
    16021 aaatattaga agaatattaa ataagtagag gtgccaacat gacatttgaa gagaagctta
    16081 gcaaaatata caatgaaatt gcgaatgaga ttagcagtat gataccggta gagtgggaaa
    16141 aagtatatac aatggcttat atagatgatg gaggaggtga agtattcttt aattatacta
    16201 aaccaggtag tgatgacttg aattattaca ccaatatacc taaggagtat aacatttctg
    16261 tgcaagtatt tgatgattta tggatggatt tatatgattt atttgaggag ttaagagatt
    16321 tatttaaaga agaagattta gaaccatgga catcatgcga atttgatttt acaagagaag
    16381 gtgaattaaa agtttcattt gattatattg attggataaa ttcaga
//
LOCUS       DAMQYT010000037        16406 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMQYT010000037 DAMQYT010000000
VERSION     DAMQYT010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16406
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.037"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            27..875
                     /locus_tag="PGA33_000959"
     CDS             27..875
                     /locus_tag="PGA33_000959"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDG6472786.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            889..1713
                     /gene="purR"
                     /locus_tag="PGA33_000960"
     CDS             889..1713
                     /gene="purR"
                     /locus_tag="PGA33_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDG6472787.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            1730..2110
                     /locus_tag="PGA33_000961"
     CDS             1730..2110
                     /locus_tag="PGA33_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDG6472788.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNIDGHIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            2182..2484
                     /gene="spoVG"
                     /locus_tag="PGA33_000962"
     CDS             2182..2484
                     /gene="spoVG"
                     /locus_tag="PGA33_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDG6472789.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            2674..2805
                     /locus_tag="PGA33_000963"
     CDS             2674..2805
                     /locus_tag="PGA33_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472790.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            2828..4180
                     /gene="glmU"
                     /locus_tag="PGA33_000964"
     CDS             2828..4180
                     /gene="glmU"
                     /locus_tag="PGA33_000964"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDG6472791.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            4327..5292
                     /locus_tag="PGA33_000965"
     CDS             4327..5292
                     /locus_tag="PGA33_000965"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDG6472792.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            5442..6095
                     /locus_tag="PGA33_000966"
     CDS             5442..6095
                     /locus_tag="PGA33_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDG6472793.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            6406..6978
                     /locus_tag="PGA33_000967"
     CDS             6406..6978
                     /locus_tag="PGA33_000967"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDG6472794.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            6978..10484
                     /gene="mfd"
                     /locus_tag="PGA33_000968"
     CDS             6978..10484
                     /gene="mfd"
                     /locus_tag="PGA33_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDG6472795.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNVEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            10474..12000
                     /locus_tag="PGA33_000969"
     CDS             10474..12000
                     /locus_tag="PGA33_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDG6472796.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDGHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLYAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRITGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            12000..13193
                     /locus_tag="PGA33_000970"
     CDS             12000..13193
                     /locus_tag="PGA33_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDG6472797.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHNQFEDVYIDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVIFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEK
                     GESNT"
     gene            13190..13453
                     /locus_tag="PGA33_000971"
     CDS             13190..13453
                     /locus_tag="PGA33_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDG6472798.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            13471..13863
                     /locus_tag="PGA33_000972"
     CDS             13471..13863
                     /locus_tag="PGA33_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDG6472799.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            13968..14369
                     /locus_tag="PGA33_000973"
     CDS             13968..14369
                     /locus_tag="PGA33_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDG6472800.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            14549..15844
                     /gene="tilS"
                     /locus_tag="PGA33_000974"
     CDS             14549..15844
                     /gene="tilS"
                     /locus_tag="PGA33_000974"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDG6472801.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE"
     gene            15849..16388
                     /gene="hpt"
                     /locus_tag="PGA33_000975"
     CDS             15849..16388
                     /gene="hpt"
                     /locus_tag="PGA33_000975"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDG6472802.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
BASE COUNT         5894 a         2229 c         3305 g         4978 t
ORIGIN      
        1 ttaagataac gaaaagaggg cggaaaatga tatatgaaac ggcaccagcc aaaattaatt
       61 ttacgctcga tacacttttt aaaagaaatg atggctatca tgagattgaa atgataatga
      121 caacagttga tttaaatgat cgtttaactt ttcataaaag aaaagatcga aagatagttg
      181 ttgagattga acataattat gtgccttcta atcataaaaa tctcgcatat cgtgcagcgc
      241 aactatttat tgagcaatat caactaaagc aaggtgtaac aatttctatc gataaagaaa
      301 tacctgtttc tgctggctta gctggaggtt cggctgatgc agcagcaacg ttaagaggat
      361 tgaatcgact ttttgatata ggggcgagtt tggaagaatt ggctctacta ggcagtaaaa
      421 tcgggacaga tattccgttt tgtatttata ataaaactgc actatgtact ggaagaggag
      481 agaaaatcga gtttttaaat aaaccacctt cagcttgggt gattcttgct aaaccaaact
      541 taggcatatc atcaccagat atatttaagt tgattaattt agataagcgt tacgacgtac
      601 atacgaaaat gtgttatgag gccttagaaa atcgagatta tcaacaatta tgtcaaagtt
      661 tgtctaatcg attagagcca atttctgttt caaaacaccc acaaatcgat aaattaaaaa
      721 ataatatgtt gaaaagtggt gcagatggtg cgttaatgag tggaagcgga cctactgtgt
      781 atgggctagc acgaaaagaa agccaagcaa aaaatattta taatgcagtt aacggttgtt
      841 gtaatgaagt gtacttagtt agactattag gatagaaggg ttgaaaagat gagatataaa
      901 cgaagcgaga gaattgtttt tatgacgcaa tatttgatga accatccgaa taaattgatt
      961 ccattaactt tttttgtgaa aaaatttaaa caggcgaagt cttcaataag tgaagatgtc
     1021 caaattataa aaaatacatt ccaaaaagaa aagttaggta cagtaattac tactgctggc
     1081 gcaagtggtg gtgttacgta taaaccaatg atgagtaaag aagaggcgac tgaagttgtt
     1141 aatgaggtca ttactctatt agaagagaaa gaacgtttgt tacctggcgg atatttattt
     1201 ttatcagatt tggtaggtaa tccatcgcta ctaaacaaag ttggtaagtt aattgccagt
     1261 atttacatgg aagaaaaatt agatgctgtt gttaccattg cgacaaaagg tatttcattg
     1321 gcaaatgcgg ttgctaatat tttaaattta ccagtagtag tgattagaaa agacaacaag
     1381 gtgactgaag gttctacagt ttcaattaat tacgtttcag gatcttcaag aaaaatagaa
     1441 acaatggtac tttcgaagag aactttagca gaaaattcaa atgttttagt tgtcgatgat
     1501 tttatgaggg ctggtggctc tattaatggt gttatgaatt taatgaatga gtttaaagcc
     1561 catgtaaaag gggtatcagt acttgtagaa tcaaaagaag ttaaacaaag attgattgaa
     1621 gattatactt ccttagtgaa attatctgat gtagatgaat ataatcaaga gtttaacgta
     1681 gaacctggca acagtttatc taagttttca taaaaggagt tttagtatta tgaaaatcat
     1741 taacacaaca agattaccgg aagcacttgg accatattcg catgcaacag ttgtgaatgg
     1801 tatggtttat acttctggtc agattccatt gaatattgat ggacatatcg taagcgctga
     1861 tgttcaagca cagacaaaac aagttttaga aaatttaaag gttgttttgg aagaagcagg
     1921 atctgatttg aattctgttg cgaaagcgac cattttcatt aaagatatga atgatttcca
     1981 aaaaataaat gaagtgtatg gtcaatattt taatgaacac aagccagcgc gtagttgtgt
     2041 agaggttgcg cgtttgccaa aagatgtgaa agtagaaatt gaattagtaa gtaaaattaa
     2101 ggaattataa ttttcgatta atatgtttaa tcaagcttct aaataaaaca gagagatata
     2161 tactataggg gggctcacta catgaaagtg acagatgtaa gacttagaaa aatacaaaca
     2221 gatggacgaa tgaaagcact cgtttccatt acattagatg aagctttcgt aattcatgat
     2281 ttacgtgtaa ttgaaggaaa ctctggcttg ttcgttgcaa tgccaagtaa acgtacacca
     2341 gatggtgaat tccgcgacat cgcgcatcct attaattcag atatgagaca agaaattcaa
     2401 gatgcagtga tgaaagtata tgatgaaaca gatgaagtag taccagataa aaacgctaca
     2461 tcagaagatt cagaagaagc ttaatcaatt ttatatttag cgatgtaata catttgcaat
     2521 aagttgattt gatactgtcg ataaagcata aagctttgtc ggcagttttt ttagtttgta
     2581 ttaatgtttt tttattttta atgaaaggct aataaatata tacgttaaca gattatgatg
     2641 atatgaaaat tattgattca ttgctgataa aaattgcgtt tgaatgatgc tcgtattttt
     2701 gaagtaagaa aaaagttgtt tttaaaatta caacgaatta aaaacaatgc cttttatatg
     2761 ttgaaagagt attgcagatt aaattataat aatgacgaag tgtaaaattt aatgggggtt
     2821 aatgttcatg cgaagacacg cgataatttt ggcagcaggt aaaggcacaa gaatgaaatc
     2881 taaaaagtat aaagtgctac acgaggttgc tgggaaacct atggtcgaac atgtattgga
     2941 aagtgtgaaa ggctctggtg tcgatcaagt tgtaaccatc gtaggacatg gtgctgaaag
     3001 tgtaaaagga catttaggcg agcgttcttt atacagtttt caagaggaac aactcggtac
     3061 tgcgcatgca gtgcaaatgg cgaaatcaca cttagaagac aaggaaggta cgacaatcgt
     3121 tgtatgtggt gacacaccgc tcatcacaaa ggaaacatta gaaacattga ttgcgcatca
     3181 cgaggatgct aatgctcaag caactgtatt atctgcatcg attcaacaac catatggata
     3241 cggaagaatc gttcgaaatg cgtcaggtcg tttagaacgc atagttgaag agaaagatgc
     3301 aacgcaagct gaaaaggata ttaatgaaat tagttcaggt atttttgcgt ttaataataa
     3361 aacgttgttt gaaaaattaa cacaagtgaa aaatgataat gcgcaaggtg aatattacct
     3421 ccctgatgta ttgtcgttaa ttttaaatga tggcggcatc gtagaagtct atcgtaccaa
     3481 tgatgttgaa gaaatcatgg gtgtaaatga tcgtgtaatg cttagtcagg ctgagaaggc
     3541 gatgcaacgt cgtacgaatc attatcacat gctaaatggt gtgacaatca tcgatcctga
     3601 cagcacttat attggtccag acgttacaat tggtagtgat acagtcattg aaccaggcgt
     3661 acgaattaat ggtcgtacag aaattggcga agatgttgtt attggtcagt actctgaaat
     3721 taacaatagt acgattgaaa atggtgcatg tattcaacag tctgttgtta atgatgctag
     3781 cgtaggagcg aatactaagg tcggaccgtt tgcgcaattg agaccaggcg cgcaattagg
     3841 tgcagatgtt aaggttggaa attttgtaga aattaaaaaa gcagatctta aagatggtgc
     3901 caaggtttca catttaagtt atattggcga tgctgtaatt ggcgaacgta ctaatattgg
     3961 ttgcggaacg attacagtta actatgatgg tgaaaataaa tttaaaacta tcgtcggcaa
     4021 agattcattt gtaggttgca atgttaattt agtagcacct gtaacaattg gtgatgatgt
     4081 attggtggca gctggttcca caatcacaga tgacgtacca aatgacagtt tagctgtggc
     4141 aagagcaaga caaacaacaa aagaaggata taggaaataa tcatttacgt atttaaaatg
     4201 gctaggataa aaggataatc ctatgtaata ttaatgtaat ctttatgatt taatgattcg
     4261 catagtaatg gagttacatt ttatatataa tagtaattgc gtaagtaaat aattggagga
     4321 ctataaatgt taaataatga atataagaat tcgtcattaa agattttttc attgaaagga
     4381 aacgaagcat tagcgcaaga agttgctgac caagtaggaa ttgaactagg taaatgttca
     4441 gttaaacgtt ttagtgatgg agaaattcaa attaatatcg aagagagtat tcgtggttgt
     4501 gacgtattta ttattcaacc aacatcatat cctgtgaatc tacatttaat ggaattatta
     4561 attatgattg atgcttgtaa acgtgcttct gcagcaacaa tcaatattgt agtgccatat
     4621 tatggatatg caagacaaga tagaaaagcc cgtagccgtg agccaatcac tgctaaatta
     4681 gttgcaaact taatcgaaac agctggcgca actcgtatga ttgcgttaga cttacatgca
     4741 ccacaaattc aaggattctt tgatattcca attgaccact taatgggtgt gccaattctt
     4801 gctaaacatt tcaaagatga tccgaatatt aacccagaag aatgtgtcgt tgtttcacca
     4861 gaccatggcg gcgttacacg tgcacgtaaa ttagctgaca ttttaaaaac tccaattgca
     4921 attatagata aacgtcgtcc tagaccaaat gttgctgaag tgatgaacat tgttggtgag
     4981 attgaaggac gtacggcaat tattattgac gatattattg atacagcagg tacaatcact
     5041 ttagctgcac aagcattaaa agataaaggt gctaaagaag tatatgcttg ttgtacacac
     5101 cctgttttat caggaccggc taaagaacgt atcgaaaatt ctgctataaa agaattaatc
     5161 gtaacaaact caattcattt agatgaagat cgcaaaccat ctaacactaa agaattatct
     5221 gttgctggtt taatcgcaca agctatcatt cgtgtatacg aaagagaatc agttagcgta
     5281 ttatttgact aatatttaaa aggcgtttga cgaacatatt ccaaacgtgt ataatagttt
     5341 cgttcgtgat tatacgaata aataaacact tgcaagcaac gatgatgttg atgggtaagt
     5401 gaggtgctcg ttttgagcaa aaatgaaagg tggaaatgag aatggcttca ttaaagtcaa
     5461 tcatccgtca aggtaaacaa acacgttcag atcttaaaca attaagaaaa tctggtaaag
     5521 taccagcagt agtatacggt tacggtacta aaaacgtgtc agttaaagtt gatgaagtag
     5581 aattcatcaa agttatccgt gaagtaggtc gtaacggtgt tatcgaatta ggcgttggtt
     5641 ctaaaactat caaagttatg gttgcagact accaattcga tccacttaaa aaccaaatta
     5701 ctcacattga cttcttagca atcaatatga gtgaagaacg tactgttgaa gtaccagttc
     5761 aattagttgg tgaagcagta ggcgctaaag aaggcggcgt agttgaacaa ccattattca
     5821 acttagaagt aactgctact ccagacaata ttccagaagc aatcgaagta gacattactg
     5881 aattaaacat taacgacagc ttaactgttg ctgatgttaa agtaactggc gacttcaaaa
     5941 tcgaaaacga ttcagctgaa tcagtagtaa cagtagttgc tccaactgaa gaaccaactg
     6001 aagaagaaat cgaagctatg gaaggcgaac aacaaactga agaaccagaa gttgttggcg
     6061 aaagcaaaga agacgaagaa aaaactgaag agtaatttta atctgttaca ttaaagtttt
     6121 tatactttgt ttaacaagca ctgtgcttat tttaatataa gcatggtgct ttttgtgtta
     6181 ttataaagct taattaaact ttattacttt gtactaaagt ttaattaatt ttagtgagta
     6241 aaagacatta aactcaacaa tgatacatca taaaaatttt aatgtactcg attttaaaat
     6301 acatacttac taagctaaag aataatgata attgatggca atggcggaaa atggatgttg
     6361 tcattataat aataaatgaa acaattatgt tggaggtaaa cacgcatgaa atgtattgta
     6421 ggtctaggta atataggtaa acgttttgaa cttacaagac ataatatcgg ctttgaagtc
     6481 gttgattata ttttagagaa aaataatttt tcattagata aacaaaagtt taaaggtgca
     6541 tatacaattg aacgaatgaa cggcgataaa gtgttattta tcgaaccaat gacaatgatg
     6601 aatttgtcag gtgaagcagt tgcaccgatt atggattatt acaatgttaa tccagaagat
     6661 ttaattgtct tatatgatga tttagattta gaacaaggac aagttcgctt aagacaaaaa
     6721 ggaagtgcgg gcggtcacaa tggtatgaaa tcaattatta aaatgcttgg tacagaccaa
     6781 tttaaacgta ttcgtattgg tgtgggaaga ccaacgaatg gtatgacggt acctgattat
     6841 gttttacaac gcttttcaaa tgatgaaatg gtaacgatgg aaaaagttat cgaacacgca
     6901 gcacgcgcaa ttgaaaagtt tgttgaaaca tcacgatttg accatgttat gaatgaattt
     6961 aatggtgaag tgaaataatg acaatattga caacgcttat aaaagaagat aatcattttc
     7021 aagaccttaa tcaggtattt ggacaagcaa acacactagt aactggtctt tccccgtcag
     7081 ctaaagtgac gatgattgct gaaaaatatg cacaaagtaa tcaacagtta ttattaatta
     7141 ccaataattt ataccaagca gataaattag aaacagattt acttcaattt atagatgctg
     7201 aagaattgta taagtatcct gtgcaagata ttatgaccga agagttttca acacaaagcc
     7261 ctcaactgat gagtgaacgt attagaactt taactgcgtt agctcaaggt aagaaagggt
     7321 tatttatcgt tcctttaaat ggtttgaaaa agtggttaac tcctgttgaa atgtggcaaa
     7381 atcaccaaat gacattgcgt gttggtgagg atatcgatgt ggaccaattt cttaacaaat
     7441 tagttaatat ggggtacaaa cgggaatccg tggtatcgca tattggtgaa ttctcattgc
     7501 gaggaggtat tatcgatatc tttccgctaa ttggggaacc aatcagaatt gagctatttg
     7561 ataccgaaat tgattctatt cgggattttg atgttgaaac gcagcgttcc aaagataatg
     7621 ttgaagaagt cgatatcaca actgcaagtg attatatcat tactgaagaa gtgatcagcc
     7681 atcttaaaga agagttaaaa actgcatatg aaaatacaag acccaaaata gataaatcag
     7741 tgcgcaatga tttgaaagaa acgtatgaaa gctttaaatt attcgaaagt acatactttg
     7801 atcatcaaat actacgtcgc ttagtagcgt ttatgtatga aacaccttcg acaattattg
     7861 agtatttcca aaaagatgca atcattgcag ttgatgaatt taatcgtatt aaagaaactg
     7921 aagaaagttt aacagtagag tctgattcgt ttattagcaa tattattgaa agtggtaatg
     7981 gatttatagg acaaagtttt ataaaatatg atgattttga aacattgatt gaaggctatc
     8041 ctgtcactta tttttcatta ttcgctacaa caatgccgat aaaactaaat catattatta
     8101 aattttcatg taaacctgtc caacaatttt atgggcaata tgacattatg cgttctgaat
     8161 ttcaacgata tgttaatcaa aactatcata tcgtggtttt ggtcgaaacc gaaactaaag
     8221 ttgaacgtat gcaagcgatg ttaagtgaaa tgcatattcc atcaataaca aaattgcatc
     8281 gctcaatgtc atcggggcaa gcagtgatta ttgaaggcag tttatctgaa ggatttgaac
     8341 tacctgatat gggattagtt gtcattactg agcgtgagct ttttaaatca aaacagaaaa
     8401 agcaacgaaa acgtacgaaa gctatctcaa atgctgaaaa aattaagtct taccaagatt
     8461 taaatgtggg agattatatt gttcatgtgc atcatggtgt tggtagatat ttaggtgttg
     8521 agacgctcga agtggggcaa acgcatcgtg attatattaa attgcaatat aaaggtacgg
     8581 atcaactatt tgttccagta gatcaaatgg atcaagttca aaaatatgta gcttcggaag
     8641 ataagacgcc aaaattaaat aaactcggtg gcagtgaatg gaaaaaaaca aaagctaaag
     8701 ttcaacaaag tgttgaagat attgctgaag agttgattga tttatataaa gaaagagaaa
     8761 tggcagaagg ttatcaatat ggggaagaca cagctgagca aacaacattt gaattagatt
     8821 ttccatatga acttacgcct gaccaagcta aatctatcga tgaaattaaa gatgacatgc
     8881 aaaaatcgcg tccaatggat cgcttgctat gtggtgatgt tggttatggt aaaactgaag
     8941 ttgcagtgag agcagcattc aaagctgtaa tggaaggaaa gcaggttgca tttttagttc
     9001 ctacaactat tttagctcag caacattatg agacgttaat tgagcgtatg caagattttc
     9061 ctgttgaaat tcaattaatg agtcgtttta gaacgcctaa agagataaaa caaactaagg
     9121 aaggacttaa aactggattt gttgacatag ttgttggtac acacaaatta cttagtaaag
     9181 atatacagta taaagattta gggctgttga ttgtagatga agaacaacga tttggtgtac
     9241 gccataaaga gcgtattaaa acattaaaac ataatgtaga tgtactaaca ttgactgcaa
     9301 ccccaatacc tagaacattg catatgagta tgctaggtgt gcgggatttg tcagtgattg
     9361 aaacgccgcc agaaaatcgt ttcccagttc aaacatatgt attagaacag aacatgagtt
     9421 ttatcaaaga agctttagaa agagaactat cccgtgatgg ccaagtgttt tatctttata
     9481 ataaagtgca atccatttat gaaaaacgag aacaactcca gatgttaatg ccagatgcta
     9541 acattgcagt tgctcatgga caaatgacag agcgcgattt agaagaaacg atgttaagtt
     9601 ttatcaataa tgaatatgat attttagtaa cgacgacgat tattgaaaca ggtgtcgatg
     9661 tcccaaatgc aaatactttg atcattgaag atgcagatcg ctttggattg agtcagttgt
     9721 atcaattaag aggtcgtgtt ggtcgttcaa gtcgtattgg ttatgcatac ttcttacatc
     9781 cagcaaataa ggtactaact gagactgcag aagatcgatt acaagcgatt aaagaattta
     9841 cggagttagg ctcaggattt aagattgcga tgcgtgattt gaacattcgt ggtgctggta
     9901 atttgttagg taaacaacag cacggcttta ttgatacagt tggatttgat ttgtacagtc
     9961 aaatgttaga agaagctgta aatgaaaaac gtggtattaa ggaaccagaa tctgaggtgc
    10021 cagaagtcga agttgattta aacttggatg catatttgcc aacagaatat attgcaaatg
    10081 aacaagctaa aattgaaatt tataaaaagc tacgaaaaac tgaaacattt gatcaaatta
    10141 tcgacattaa agatgaatta attgatcgtt tcaatgatta tcctgttgaa gtagcacgtt
    10201 tgcttgatat agtggaaata aaagtacacg cattacattc aggtatcacg ttgattaaag
    10261 ataaagggaa aataattgat attcatttat ctgtaaaagc cactgaaaat attgatggcg
    10321 aagtgctgtt caaagcaaca caacctttag gtagaacaat gaaggttggt gttcaaaata
    10381 atgcaatgac aattacttta acgaaacaaa atcaatggct tgatagtttg aagtttttag
    10441 ttaagtgcat tgaagaaagt atgagaatca gtgatgaagc ataaagaagc atttaatggc
    10501 gttgtcgtgt taactgctgc attaattgtc attaaaattc tgagtgctgt atatcgaatt
    10561 ccatatcaaa atatattagg cgatacaggt ttgtatgcat atcaacaagt gtatccaatt
    10621 gtagcattag gaatgatatt atcgatgaat gccattccta gtgcaattac acaaaatata
    10681 gggaagtatc atagtgacga agcatatgca aaagcagtcg cttatataca attagttggt
    10741 atattattat ttattgctat ttttgtgttt gcgaacaata ttgcacatat gatgggtgat
    10801 ggccatttaa caccaatgat tcaagctgca agtttaagct ttatatttat aggtatgctt
    10861 ggcgtgttaa gaggttatta tcaatctgca aataatatga cagttccggc tatttcccag
    10921 gttatagaac aagttatacg agtaggtatt atcattgtta ctattgttat ttttgtagac
    10981 agaggttgga cgatatatga agcgggaaca attgctattt tagcatcaac gataggtttt
    11041 ttaggttctt caatttattt agtagcgcac cgacctttta agtttaaaat ggtaaataac
    11101 actgcaaaga tcgtttggaa acagttcgca ctttcggttt tgattttcgc tatcagtcaa
    11161 ttaatcgtaa ttttatggca agtgattgat agtgttacta ttattaagtc acttcaagcg
    11221 atacgcgtgc cattcgatgt tgccataact gaaaaaggag tctatgaccg tggtgcatca
    11281 tttattcaga tgggattgat tgtaactaca acatttagtt ttgcgctcat tcctctgtta
    11341 agtgacgcaa ttaaaatgaa taatcaggta cttatgaatc gttatgcaaa tgcgtcatta
    11401 aagattacga ttttaataag tacagcagca ggaataggat taattaattt attgccttta
    11461 atgaacggtg tgttttttaa gacgaatgat ttaaccttaa cgttaagtgt ttatatgatt
    11521 acggtcattt gtgtatcgtt aattatgatg gatatggcat tattacaagc gcaacatgct
    11581 gtgagaccta tttttgttgg tatgacggca ggattggtta ttaaatttat acttaatatc
    11641 attttgattc gtttaagtgg cattattggt gcgagcatta gtactgttgt atcattaatt
    11701 atattcggta cgattatcca tattgctgtc acgagaaaat accacttata tgcgatgaga
    11761 cgatttttta tcaatgttgt tttaggtatg gtatttatgt cgattgttgt tcaatgcgtg
    11821 ttaaacatag tgacaacaca cggtagaatc actggactca ttgaattatt atgtgcagca
    11881 gtattaggta tcattgcatt gtttttctat atttttagat ttaatgtttt gacatataaa
    11941 gagttaactt atttaccatt tggttcaaag ttgtatcaaa ttaagaaagg aagacgttga
    12001 tggcacatac cattacgatt gttggcttag gaaactatgg cattgatgat ttgccgctag
    12061 ggatatataa atttttaaag acacaagata aagtttatgc aagaacgtta gatcatccag
    12121 ttatagaatc attgcaagat gaattaacat ttcagagttt tgaccatgtt tatgaagcac
    12181 ataaccaatt tgaagatgtc tatattgata ttgtggcgca attggttgaa gctgctaatg
    12241 aaaaagatat tgtctatgcg gttccgggtc atcctagagt tgctgagaca actacagtga
    12301 aattactggc tttagcaaag gacaatactg atatagatgt gaaagtttta ggtgggaaaa
    12361 gctttattga tgatgtgttt gaagcagtta atgtagatcc aaatgatggc ttcacactgt
    12421 tagatgcgac atcattacaa gaagtaacac ttaatgttag aacgcataca ttgattacgc
    12481 aagtttatag tgcaatggtt gctgctaatt tgaaaatcac tttaatggaa cgatatcctg
    12541 atgattaccc tgttcaaatt gtcactggtg cacgaagcga tggtgcggat aacgttgtga
    12601 catgcccatt atatgaattg gatcatgatg aaaatgcatt caataatttg acgagtgtat
    12661 tcgtaccaaa aatcataaca tcgacatatt tgtatcatga ctttgatttt gcaacggaag
    12721 tgattgatac tttagttgat gaagataaag gttgtccatg ggataaagtg caaacgcatg
    12781 aaacgctaaa gcgttattta cttgaagaaa catttgaatt gttcgaagct attgacaatg
    12841 aagatgattg gcatatgatt gaagaactag gagatatttt attacaagtg ttattgcata
    12901 ctagtattgg taaaaaagaa gggtatatcg acattaaaga agtgattaca agtcttaatg
    12961 ctaaaatgat tcgtagacac ccacacatat ttggtgatgc caatgctgaa actatcgatg
    13021 acttaaaaga aatttggtct aaggcgaaag atgctgaagg taaacagcca agagttatat
    13081 ttgaaaaagt atttgcagag cattttttaa atttatatga gaagacgaag gataagtcat
    13141 ttgatgaggc cgcgttaaag cagtggctag aaaaagggga gagtaataca tgagattaga
    13201 taaatattta aaagtatcac ggttaataaa gcgacgtacg ctagcaaaag aagtaagtga
    13261 tcaaggtaga attacaataa atggtaatgt tgctaaagct ggatcggatg ttaaagttga
    13321 agatgtgctg acgattcgct ttggtcaaaa attagtaaca gttaaagtaa ctgcattaaa
    13381 tgaacatgca tctaaagata acgcgaaggg tatgtatgaa atcattgaag agcgtcgact
    13441 tgaagaagcg taaattggag gtgacaagca atgaaaaata aagtagaaca tatagaaaat
    13501 cagtacacgt cgcaagagaa caagaaaaaa caacgtcaaa aaatgaaaat gcgtgttgtt
    13561 cgtaggcgta ttacagtatt tgcgggcgta ttacttgcga taattgttgt tttatcaatc
    13621 ttgcttgttg tccaaaaaca tcgcaatgat attgatgcac aggagcgaaa agcgaaagaa
    13681 gcacagtttc aaaagcaaca aaatgaagaa attgcgttaa aagaaaagtt gaataatctg
    13741 aatgacaaag attacattga aaaaattgcg cgtgatgatt attacttaag caacaaaggt
    13801 gaagtgattt ttaggttgcc agaagacaaa gattcgtcta gctcaaaatc ttcgaaaaaa
    13861 taaatccaaa ttgattcaaa attatccgag tatagacatt gtgaaaaaat ccaaacaagg
    13921 atataataag ggaaaatcga atcaaatcgg gaggatttat ttaacatatg tcaatcgaag
    13981 ttggaaataa gcttaaaggt aaagtcactg gtattaaaaa gtttggtgca ttcgtagaat
    14041 tacctgaagg aaaaagtggt ttagttcaca ttagtgaagt cgcagataat tatgttgaaa
    14101 acgtagaaga gcacctttct gttggtgatg aagtagacgt aaaagtatta tctattgctg
    14161 atgatggaaa aattagtctt tcaattaaga aagctaaaga ccgtccacgt agacaacata
    14221 cgagtaaacc aagtcatcaa aaaccagtgc aaaaagccga agattttgaa aagaaattaa
    14281 gcaatttctt aaaagatagt gaagataaat taacttcaat caaacgtcaa acagaatcta
    14341 gacgcggtgg caaaggttca agacgttaat taaaataaat aaagactgct tcgataagga
    14401 atatatttag aatgatgcgt atcgaataat cgattgcagc gttagacaat ctaagactgt
    14461 ttcttaaata aggagcagtc tcttttattt gtaatgatat aactaagact tataccattt
    14521 ttgaaaattg taaaagtgag gtgatgttat gcagttaaat agtaatggtt ggcatgttga
    14581 tgaccatatt gttgtcgctg tttctacagg tattgatagt atgtgtttat tgtatcaact
    14641 actaaatgat tataaagata gttatagaaa actaacatgc ttacatgtca atcatggcgt
    14701 taggtcagct tcaattgagg aagccagatt tttagaagca tactgcgaac gtcatcacat
    14761 cgatttacat atcaaaaagt tagatttgtc gcatagtctc gaccgaaata acagcattca
    14821 gaatgaagct cgaattaaac gttacgaatg gtttgatgaa atgatgaatg tattagaagc
    14881 ggatgtattg ctaacggcgc atcatttgga cgatcaatta gaaactatta tgtatcgtat
    14941 ttttaatggg aaatcaacac gtaataaact aggatttgat gagttatcga agcgaaaagg
    15001 ttatcagatt tatcgaccac ttttagctgt ctctaaaaaa gaaataaaac aattccaaga
    15061 gagatatcat attccatatt ttgaagatga atctaataaa gataacaaat atgttagaaa
    15121 tgatattcgt aatagaatta ttccagctat tgatgaaaat aatcaactta aagtatcgca
    15181 tttattaaaa ttaaaacaat ggcatgatga acaatatgat attttgcaat attcagctaa
    15241 acaatttatt caagaatttg tgaagtttga tgaacagtca aaatatttag aggtttctag
    15301 acaagctttt aataacttac caaactcatt aaagatggtt gtgttggact gcctattatc
    15361 aaagtattat gagttgttta atattagtgc taaaacatac gaagagtggt ttaaacaatt
    15421 tagtagtaag aaagcacaat tcagtattaa tctcacggat aaatggataa ttcaaatcgc
    15481 atatggtaaa ttaataataa tggctaaaaa taatggcgat acatatttta gagttcaaac
    15541 aattaaaaag ccaggtaatt atatttttaa caaatatcga ttagagatac attctaattt
    15601 accaaaatgt ttatttccgc ttacagtgag aacacgacaa agtggcgata catttaaact
    15661 gaatgggcgc gatggttata agaaagtgaa tcgcctgttt atagattgta aagtgccaca
    15721 gtgggttcgg gatcaaatgc caatcgtatt ggataaacaa cagcgcatta ttgcggtagg
    15781 agatttatat caacaacaaa caataaaaaa atggattata attagtaaaa atggagatga
    15841 atagcgttat gcataatgat ttgaaagaag tattgttaac tgaagaagat attcaaaata
    15901 tctgtaagga attgggagca caattaacaa aggattatca aggtaaacca ttagtatgcg
    15961 tgggtatctt aaaaggctca gcaatgttta tgtcagattt aattaaacga attgataccc
    16021 atttatcaat tgatttcatg gatgtttcta gttatcacgg aggcactgag tcaactggtg
    16081 aagttcaaat cattaaagat ttaggttctt ctattgaaaa taaagacgta ttaattattg
    16141 aagatatctt agagactggt actacactta agtcaattac tgaattatta caatctagaa
    16201 aagttaattc attagaaata gttactttat tagataaacc aaaccgtcgt aaagcggaca
    16261 ttgaagctaa gtatgtaggt aaaaaaatac cagatgaatt tgttgttggt tacggtttag
    16321 attatcgtga attataccga aacttaccat atatcggtac gttaaaacct gaagtgtatt
    16381 caaattaatt ttttaatcaa tttcag
//
LOCUS       DAMQYT010000038        16281 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMQYT010000038 DAMQYT010000000
VERSION     DAMQYT010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16281
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.038"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            31..2718
                     /gene="harA"
                     /locus_tag="PGA33_000976"
     CDS             31..2718
                     /gene="harA"
                     /locus_tag="PGA33_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HDG6472803.1"
                     /translation="MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKNYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVVTNDQSSSVASNQTNTNTSNQNTSTINNANNQPQATTN
                     MSQPAQPKSSTNADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVA
                     DTDVAENSSTATNPKDASDKADVIEPESDVVKDADNNIDKDVQHDVDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDKDADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNN
                     HTGKAAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLS
                     QSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            2849..2941
                     /locus_tag="PGA33_000977"
     CDS             2849..2941
                     /locus_tag="PGA33_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472804.1"
                     /translation="MKIILLLFLIFGFIVVVTLKSEHQLTLFSI"
     gene            complement(2921..3826)
                     /locus_tag="PGA33_000978"
     CDS             complement(2921..3826)
                     /locus_tag="PGA33_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDG6472805.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
     gene            4208..5470
                     /locus_tag="PGA33_000979"
     CDS             4208..5470
                     /locus_tag="PGA33_000979"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDG6472806.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(5564..6838)
                     /locus_tag="PGA33_000980"
     CDS             complement(5564..6838)
                     /locus_tag="PGA33_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDG6472807.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            7010..7627
                     /locus_tag="PGA33_000981"
     CDS             7010..7627
                     /locus_tag="PGA33_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDG6472808.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            7755..9221
                     /locus_tag="PGA33_000982"
     CDS             7755..9221
                     /locus_tag="PGA33_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HDG6472809.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGVVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDK
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            9328..10458
                     /locus_tag="PGA33_000983"
     CDS             9328..10458
                     /locus_tag="PGA33_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDG6472810.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(10419..10514)
                     /locus_tag="PGA33_000984"
     CDS             complement(10419..10514)
                     /locus_tag="PGA33_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472811.1"
                     /translation="MKPLVQSFTTSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(10608..12212)
                     /locus_tag="PGA33_000985"
     CDS             complement(10608..12212)
                     /locus_tag="PGA33_000985"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDG6472812.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHTLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAV
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(12199..13359)
                     /locus_tag="PGA33_000986"
     CDS             complement(12199..13359)
                     /locus_tag="PGA33_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDG6472813.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINFLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKEHFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(13473..13919)
                     /locus_tag="PGA33_000987"
     CDS             complement(13473..13919)
                     /locus_tag="PGA33_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDG6472814.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPQI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            13999..14742
                     /locus_tag="PGA33_000988"
     CDS             13999..14742
                     /locus_tag="PGA33_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDG6472815.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVTRKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            complement(14958..15560)
                     /gene="rpsD"
                     /locus_tag="PGA33_000989"
     CDS             complement(14958..15560)
                     /gene="rpsD"
                     /locus_tag="PGA33_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDG6472816.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(15804..16268)
                     /locus_tag="PGA33_000990"
     CDS             complement(15804..16268)
                     /locus_tag="PGA33_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDG6472817.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQIVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
BASE COUNT         5515 a         2500 c         2850 g         5416 t
ORIGIN      
        1 ttaacactaa taataggagt tgttaacaac atgaacaaac atcacccaaa attaaggtct
       61 ttctattcta ttagaaaatc aactctaggc gttgcatcgg tcattgtcag tacactattt
      121 ttaattactt ctcaacatca agcacaagca gcagaaaata caaatacttc agataaaatc
      181 tcggaaaatc aaaataataa tgcaactaca actcagccac ctaaggatac aaatcaaaca
      241 caacctgcta cgcaaccagc aaacactgcg aaaaactatc ctgcagcgga tgaatcactt
      301 aaagatgcaa ttaaagatcc tgcattagaa aataaagaac atgatatagg tccaagagaa
      361 caagtcaatt tccagttatt agataaaaac aatgaaacgc agtactatca ctttttcagc
      421 atcaaagatc cagcagatgt gtattacact aaaaagaaag cagaagttga attagacatc
      481 aatactgctt caacatggaa gaagtttgaa gtctatgaaa acaatcaaaa attgccagtg
      541 agacttgtat catatagtcc tgtaccagaa gaccatgcct atattcgttt cccagtttca
      601 gatggcacac aagaattgaa aattgtttct tcgactcaaa ttgatgatgg agaagaaaca
      661 aattatgatt atactaaatt agtatttgct aaacctattt ataacgatcc ttcacttgta
      721 aaatcagata caaatgatgc agtagtaacg aatgatcaat caagttcagt cgcaagtaat
      781 caaacaaaca cgaatacatc taatcaaaat acatcaacga tcaacaatgc taataatcaa
      841 ccgcaggcaa cgaccaatat gagtcaacct gcacaaccaa aatcgtcaac gaatgcagat
      901 caagcgtcaa gccaaccagc tcatgaaaca aattctaatg gtaatactaa cgataaaacg
      961 aatgagtcaa gtaatcagtc ggatgttaat caacagtatc caccagcaga tgaatcacta
     1021 caagatgcaa ttaaaaaccc ggctatcatc gataaagaac atacagctga taattggcga
     1081 ccaattgatt ttcaaatgaa aaatgataaa ggtgaaagac agttctatca ttatgctagt
     1141 actgttgaac cagcaactgt catttttaca aaaacaggac caataattga attaggttta
     1201 aagacagctt caacatggaa gaaatttgaa gtttatgaag gtgacaaaaa gttaccagtc
     1261 gaattagtat catatgattc tgataaagat tatgcctata ttcgtttccc agtatctaat
     1321 ggtacgagag aagttaaaat tgtgtcatct attgaatatg gtgagaacat ccatgaagac
     1381 tatgattata cgctaatggt ctttgcacag cctattacta ataacccaga cgactatgtg
     1441 gatgaagaaa catacaattt acaaaaatta ttagctccgt atcacaaagc taaaacgtta
     1501 gaaagacaag tttatgaatt agaaaaatta caagagaaat tgccagaaaa atataaggcg
     1561 gaatataaaa agaaattaga tcaaactaga gtagagttag ctgatcaagt taaatcagca
     1621 gtgacggaat ttgaaaatgt tacacctaca aatgatcaat taacagattt acaagaagcg
     1681 cattttgttg tttttgaaag tgaagaaaat agtgagtcag ttatggacgg ctttgttgaa
     1741 catccattct atacagcaac tttaaatggt caaaaatatg tagtgatgaa aacaaaggat
     1801 gacagttact ggaaagattt aattgtagaa ggtaaacgtg tcactactgt ttctaaagat
     1861 cctaaaaata attctagaac gctgattttc ccatatatac ctgacaaagc agtttacaat
     1921 gcgattgtta aagtcgttgt ggcaaacatt ggttatgaag gtcaatatca tgtcagaatt
     1981 ataaatcagg atatcaatac aaaagatgat gatacatcac aaaataacac gagtgaaccg
     2041 ctaaatgtac aaacaggaca agaaggtaag gttgctgata cagatgtagc tgaaaatagc
     2101 agcactgcaa caaatcctaa agatgcgtct gataaagcag atgtgataga accagagtct
     2161 gacgtggtta aagatgctga taataatatt gataaagatg tgcaacatga tgttgatcat
     2221 ttatccgata tgtcggataa taatcacttc gataaatatg atttaaaaga aatggatact
     2281 caaattgcca aagatactga tagaaatgtg gataaagatg ccgataatag cgttggtatg
     2341 tcatctaatg tcgatactga taaagactct aataaaaata aagacaaagt catacagctg
     2401 aatcatattg ccgataaaaa taatcatact ggaaaagcag caaagcttga cgtagtgaaa
     2461 caaaattata ataatacaga caaagttact gacaaaaaaa caactgaaca tctgccgagt
     2521 gatattcata aaactgtaga taaaacagtg aaaacaaaag aaaaagccgg cacaccatcg
     2581 aaagaaaaca aacttagtca atctaaaatg ctaccaaaaa ctggagaaac aacttcaagc
     2641 caatcatggt ggggcttata tgcgttatta ggtatgttag ctttattcat tcctaaattc
     2701 agaaaagaat ctaaataatt aactaaatat agcatatgta tgattaactt tgtagacaat
     2761 gtgaaagcaa ttaatttata aactattgat tggtttaatg gctttccttt agagtaaata
     2821 aaaagaacag cagtgagaaa ttttctaatt gaaaataatc ttactgctgt ttttaatatt
     2881 tggattcatt gttgtggtta ctttaaaaag tgagcatcaa ttaacgcttt tttcgattta
     2941 acaaatgtga tttaatatca tattttaatg cgtcgttgta ttctttttca gtgatttgat
     3001 cttcgattaa catacgcttt aatacataat gttgtctttg aatactatat ttcaaatctt
     3061 tatccgattt taacgttcca tctttttcgt agggtgtata gccatatggg ctttgcaaca
     3121 aaccgataag gtatgcagat tgtgcaattg ataaatcttt tggtggaata ccaaatagac
     3181 tatatgaagc ggatgcaatt ccggaaatat tagcgccatt ataatctcta ccgaagggaa
     3241 ctatatttaa atatgtatat ataatttcat cttttgagag taggtgttct aatctaattg
     3301 ctaggcgaag ttcatttgct tttctactat atgttttttc gttggtaaga acttgatttt
     3361 taacaagttg ttgtgtaatt gtgctaccac ctgaactttg atcagtatta aaaatatctt
     3421 gtatcattgc tcttaaaatc gcctttggta agatgccatc atgtttataa aataaagtgt
     3481 cttcagatga cgttaatgct ttaatgacat ttggacttga tgttttaggg cctataatga
     3541 gtgagttttg agaatggtta tactcatata ataaattttt gttattatga tctaataatt
     3601 catcgccagg tatttgtcga acttttttta ttaaagcatc atctgataat gagtcggacg
     3661 ttttagttaa atgatgaaaa tataaagaca tcgcaatcac agcgatagca acgatggcaa
     3721 tgaaaatcat aaatataaaa acaatgacat gtttgatagc tttatatatc ttttcaaaat
     3781 gatatatacg atgattcaat tgatgattgt tgtcttgatt cgtcatcttt aacctcctaa
     3841 attacgtata gtataccata tttaagcaat ttgactttta ttttaacgtt aattataatt
     3901 agggaaaatg cataaagaat aggataaaag gacaagtaat attatttgaa atcatcagag
     3961 agctagtggt cgctgtgaac tagtgttttt aataatatga atacaccttt ataactaaca
     4021 tgtcctacgt ttattaatga acgtatcatt aatagagtga acattatctt atgattatgt
     4081 ttaagtttgg tggtaacgtg caatcgcgcc cttacatatt tgttgtaagg gtttttttta
     4141 cgttcaaaaa actttaaagt attttaacaa gtattcaata aataattata aataggagga
     4201 aataataatg acgaatgtat taattgaaga tttaaaatgg agaggtctta tttatcaaca
     4261 aactgatgaa caaggtattg aagatttatt aaataaagaa caagtgacgt tatactgcgg
     4321 tgccgatcca acggcagata gtttacatat tggtcactta ctaccattct taacattaag
     4381 acgttttcaa gaacatggac atcgtcctat cgttttaatt ggcggtggta caggtatgat
     4441 tggtgatcca tcaggtaaat cagaagaacg tgtgctacaa acagaagaac aagtagataa
     4501 aaatatcgaa ggtattagta agcaaatgca caatattttt gaatttggaa cagaccatgg
     4561 tgcagtgctt gttaataata gagactggtt aggacaaatc tcattaatta gttttttacg
     4621 tgactatggt aaacacgtcg gcgttaatta catgttaggt aaagattcaa tccaaagtcg
     4681 tttagaacat ggtatttcat atacagaatt cacatacacg attttacaag ctattgattt
     4741 cggtcatttg aatagagaat tgaattgtaa gattcaagta ggtggatcag atcaatgggg
     4801 taatatcaca agtggtattg aattaatgcg tcgtatgtat ggtcaaacag acgcatacgg
     4861 tttaactatt ccgcttgtaa ctaaatcaga tggtaagaaa tttggtaagt ctgagtcagg
     4921 tgctgtttgg ttagatgctg aaaaaacaag tccttatgaa ttttatcaat tctggattaa
     4981 tcaatcagac gaagatgtaa ttaaattctt aaaatacttt actttcttag gaaaagaaga
     5041 aattgatcgc ttagaacaat ctaaaaatga agcaccgcat ttacgtgaag ctcaaaaaac
     5101 attagctgaa gaagtaacta aatttattca tggtgaagat gcattaaatg atgcaatccg
     5161 tatttcacaa gcattattta gtggtgattt aaaatcatta tcagcgaaag aattaaaaga
     5221 tggatttaaa gatgtgcctc aagtgacatt atcaaatgac acaacaaata tcgttgaagt
     5281 ccttattgaa acaggcattt ctccttctaa acgacaagca cgtgaagatg ttaacaatgg
     5341 tgcgatttat attaatggtg agagacaaca agatgttaat tatgctttag caccagaaga
     5401 taaaattgat ggcgaattta cgattattcg tcgcggtaag aaaaaatact tcatggttaa
     5461 ctatcaataa atataattgc atagctaaat aaattagagc ctactcatat tcattcctaa
     5521 gaatgtaatg agtaggctct taatgtactt ttctgtctgt aaattatcta aagaaatctc
     5581 tatcgccttg gccaggtgat tgacgttctg atcggctttg acgttttggt tgttcttttt
     5641 gttgttttaa tttcacttta acttcttttg ttttaccatc acggataacg gtaacagtga
     5701 ctgattcacc aggtttttta ttttcatata aatagcttct taaatcaaca tcatctttaa
     5761 ttttcttgcc atcaatttct gtaataatat cacctttttt aagatcaata tcactatcag
     5821 ctttggcgac ataaataccg tcttctctat cagtatgaag ttgctcgcgc tcttcttcag
     5881 gaatatcttt caaattaatt aaaccaatac caatcgaagg gcggtcaatt ttaccatgtt
     5941 ttacaagttg ttcaattgtt actttaactt cattacttgg aatagcaaac ccgatacctt
     6001 caacttgtgt cgcagcaatt ttcattgagt taataccaac taaattacca ttaatatcta
     6061 ctaatgcgcc acctgagtta cctgggttaa tagcagcatc tgtttgaaga acgctaactt
     6121 ttgtattgcc accagttgtc tcagcgtcaa tcgtacgttc gcttgctgaa atgataccag
     6181 atgttacaga gttagcaaat tgtaatccta atgggttacc cattgcgaat acgctatcgc
     6241 cagtttgtac ttttgaagag ttggcaaatt gaatcgcttt aatacctttt gtattttcaa
     6301 ttttaagtac agcaatatca gttactgcat ctttaccaac taatttcgct ttaacttgtt
     6361 ttttattatg taattggact ctaatttcat ttgcgccatc aataacatga ttgtttgtaa
     6421 cgatataagc tgaattgttg tttatttgat agataacacc tgaacctact ccagcttcag
     6481 atggtttaga tgatttgcct tttaataagt cgtctacact tgatgctttt tgcatgttaa
     6541 taactccaac aattgtaggg gagacagatt ttatcatttc atgaacggta ccgaatttct
     6601 tgctttgacc gtctaattga ttgccacctt tattatttgt tgtctgaaca gttgaaccat
     6661 ctttatttaa aattgtacta tttaatactt tgcctatacc aagtactaga agtgcaccaa
     6721 taattccagc aatcaatgca acgatgactg ttttaaacca tggaaattta ggtcttctgt
     6781 atctaggtgt ttggctatgg tttgttgtag aatgatctgt atgattaaaa tctgacatac
     6841 ttaacctcca ttatatgatt tatatatgct ttaattatgt ctttttttta taattattgc
     6901 tagtaataca ctatacaact ttagatgtat tttcagacta tcttgttcta gtaaaaatat
     6961 agtgataaaa tctagttgtt ataaacaaat aagttaagta ggtggcacaa tgtattcagt
     7021 gattagtaag attttgaatt tcattttagt taaaatgtca aaatcattat acgtaatcgg
     7081 taaagataat attcctaagg atagtaaata tgtcgtaact tgtacgcatg aaagttataa
     7141 cgaagtcatt atgctaggta tggcattata tcctaatcaa attcattata tggctaaaaa
     7201 agaattattc aaaaataaat ggattggtaa atttttaacg tctttaaatg catttcctgt
     7261 agatcgtgaa aatccaggac ctagtacttt aaaacgccca atcaatttgt tgaaagataa
     7321 taagacagtt ggtatcttcc caacaggttc tcgcacatcg caagaaggtg cacctttgaa
     7381 acgtggtgct tcaacaattg caatgttaag taagtcacca atattacctg ttgcttatgt
     7441 tggtcctact aagattcacg gtcttttaac aggacaagca tacataaata ttggcaaacc
     7501 aatcgatatt aacgatttac ctaaagattt aaaacgaaac gagcgtatcg attatattac
     7561 aaaagagatt gaaacacgaa cagcaaagtt acaacaagaa ttgcatgaaa ttgtaaaaag
     7621 tttataaaat aacttgaggt agaaatatag ctggttagta agcctaggtg actataacgg
     7681 agtatcgaag tagttattct agttacatta gtaatcttta tatttctaca ttattttaag
     7741 aaggatgaca ttcaatgtat aaatttttcc aaaatttagg gcgttcgtta atgttaccag
     7801 ttgccatttt accggctgca gcaattattg ccggaattgg taatacgtta aatgcacttc
     7861 atgcgacgcc gaaaatcgca atgttcttta ctacggtagg tactacgatt ttagagcaat
     7921 taggtatttt atttgcaatt ggtgtagcta taggaatggc aaagaaaaat gatggtgtag
     7981 tcgcgcttgc agctacgtta ggatattttt tagttacagt tgtattatca ccaatgaaat
     8041 tagcgccttt attaggaatg aaagcttcgg aaataaattc agcttttgaa aagatgaata
     8101 atggcaatgt ctttgttggt attgtaatag gtttaatagc agcttatgca tacaataagt
     8161 ttagtgaaac agaattacca ttagcattat cattttttag tggtaaacgt ctagttccaa
     8221 ttatgactgc attttactgt acatttttag ttgtcatatt gttattctta tggccactac
     8281 tttattcatg gattgtaaaa tttggtgaat ctatagtcgg tttaggatcg tttggagctt
     8341 tcatatatgg tgttgccaat agattattaa ttcctacagg attacatcat gcactgaata
     8401 gtgtgttctg gtttgatacg attggtatta atgatattgg taaattccaa agtggcaaag
     8461 atgctataaa aggtattaca ggacgctatc aagcgggatt cttcccaatc atgatgttcg
     8521 gtattccagc ggctgcgtta gcaatgtatc atacagctaa aactactcag aaaaaacaag
     8581 tttacggttg gttcctagcg agttcagttg cagcattttt cgttggtgtt acagagccga
     8641 ttgagtttgc atttatgttt gttgcgccaa tcttatatgt tgttcatgcg ttattaacag
     8701 gattatcttt atttatcgca gctacattcc attggacagc tggattttca tttagtgcag
     8761 gtttgataga ttatgtgtta tcactaatta atccagtatc aaatcatcca ttgatgttgc
     8821 ttgttcaagg tgttgtattt ttcatcttat actatgtgat tttccgtgta gtaatccaag
     8881 tatttaactt gaatacgatt ggtagaggtg aaaatgaatt agttgaccca acagttgtaa
     8941 aagataatat tgctcctggt gaaaatgata ttaaacaaag taaatatcat caacatgcta
     9001 tacaaatatt agaaggttta ggcggtcaag agaatattgt taatttaacc aattgtgcaa
     9061 caaggttgcg tctagagtta aaagacacat ctattattga taaacagaag attaaaaacg
     9121 ccggtgcagt tggtgttact gttaatggca aacattcaac tcaagttatt gtcggtacac
     9181 acgtacagca ggttgctgat gaaattgaaa aacatttata aaatttaaac agctaaagcg
     9241 caaatctaac ttcaataggg tttgcgcttt ttttagtcaa atagtagaat gatttatatc
     9301 tgaattttca gaaatggagt ggggattgtg aagaaaattt tatttgatgt tgatggcgtt
     9361 tttttaagtg aagaacgttg tttcgatgtc tctgcattaa cggtatatga attattaatg
     9421 gataagtgtt atttaggtct tcattcacat atagattggg aaactttgac tgataacgat
     9481 atacaagaca ttaggaatcg aatttttcaa aaagataaga ttttaaacaa attaaaatcg
     9541 ctagggctga attcaaattg ggatatgtta tttatcgttt ttagtattca tttaattgat
     9601 attttgaaaa agttatcaca tgatgaaatt gaggcattca tgtatcaaga tgagcctgtc
     9661 gaattgaagt tgcaaaatat aagcacaaac ttagcagact gttttaattt aaatgaacaa
     9721 ctacctttac aatttttgga taatgtaaaa gttggtaaaa ataatattta tgctgctttg
     9781 gaagagtttg caacaacaga gttacatgtt tcggatgcta ctttatttag tttgaagggt
     9841 gcattatgga cgttagcgca agaagtttat caagaatggt atttaggatc gaagttgtat
     9901 gaagatgttg aaaagaaaat agcacgaact acttttaaga caggttatat ttatcaagaa
     9961 attattttga gaccagtaga tgaagttaag gtacttctga atgatttaaa aggtgctggt
    10021 ttcgaattag gtattgcaac aggtcgtcct tatactgaga ctgttgtgcc atttgaaaat
    10081 ttaggattgt taccatattt tgaagctgat tttattgcaa cagcaagtga tgttttagaa
    10141 gcagagaata tgtatccgca agcacgacca ttaggaaagc cgaatccttt tagttatatc
    10201 gcagctttat atggtaataa tcgcgataaa tatgaatctt atatcaataa gcaagataac
    10261 attgtaaata aagatgacgt atttatagta ggcgattcgt tagctgactt attaagtgct
    10321 caaaaaatag gtgcaacgtt tattggaaca ttaacaggtt taaaaggtaa ggatgctgca
    10381 ggtgagttag aagcgcatca tgccgactat gttattaatc atttaggtga acttagaggt
    10441 gtactagata atttgtaatt tgattgttgt ttgacagcat aacttgtagt gaatgattga
    10501 accaaaggtt tcatattgag ttacaatgaa attaataatg aaaaaatgcc aagaagcaat
    10561 ggaagtaatc caatgtcttc ttggcatttt gaatttacat aaattgttta tgactgtacc
    10621 gtcaattcag ttgtgaaaat ttgattgtat tcaccaactt gtttaagttc atcaattata
    10681 ttgtttgaaa caggttgatc aacggataaa atcattagcg catctccgcc cgcttcagtt
    10741 ctacctaaag tcatagatgc aatgttgata ttgtatttac ctaacaatgc gccagttttt
    10801 cctaccatac ctggagtatc attatgatat gacacaattt gatattgatt tggctttaag
    10861 tctacagaaa aattattaat tctaacaatt cttggaccga aacctgtaaa gacagaagcg
    10921 ccaactttaa cggaatcgct atcgtttgat agttctacct ctaagtagtt actaaaacct
    10981 gtctctgctt tattattttc aatatttaat gtcacttgtt gttgatttaa caacattaga
    11041 gcattgataa tattgacttc aggaccaaga tcatctttta aaatatgagt aataattgta
    11101 cgtgttaata aactactatc aatagaggct aagtcgccac cgtacgtaat tttaatagag
    11161 cttggtgcat tgtacattaa ttgaatagct aattcaccaa ctgtttggct taaattgatg
    11221 aatgatttta cagtatcatc tatattgctt aagtccattt taggtgcatt cactgcatgc
    11281 gttacagtac catcaattaa aatttcgatg atttcatttg aaacagaaat tgccactttt
    11341 tcttgagctt cgactgttga agcacccaaa tgaggtgtaa caataatttt atcatgtgca
    11401 acaagaggcg agtcagttgc aggttcatgt tcaaacacat cgatagctgc ccgactaatt
    11461 tgtccttcgt ctaatgcttt tattagcgcc ttttcatcaa taataccacc acgtgccaca
    11521 ttgattattt gcaaactagg ttttgctttg gcaaaaaaga cagcattaat taagcctttt
    11581 gttttaggtg ttagtggtgt atgtaatgta acgaaatcag aatgttgggc aatctcatca
    11641 actgttgcct tcgtaatgct taaagatttt gctttttcat ccgttaagta agggtcaaaa
    11701 gctagtattt tcattccgaa actttgcgca cgtttagcaa cacctaaacc aattctacca
    11761 gcaccaatga cacctaatgt tttatgataa agctcagtac ctttaaatgc atttcgattc
    11821 cattctttat ttgtaagtga ttggtgtgct tgcggaatat ttcgtgccat tgataataac
    11881 attgccagtg tatgttcagt agctgaaatc gtattaccat ctggggcatt aataactaaa
    11941 atacctttta atgttgcagc atcaatatta atattatcta caccaacacc ggcgcgtgcg
    12001 attactttta aagaatcagc agcatttatg atattttcag taaccgtagt ttgactacga
    12061 acgattaaag catggtatga aggtataatt ttgattaatg cttcttcgga caagccagtt
    12121 tgaatatcta cattgaattg ttcgtgatct aataatgctt tgataccatc ttttgatatg
    12181 ggatctgcaa cgagtacatt aaattgcttc atgaataacc tccatatatt ttgatatacc
    12241 tttaccgata tagttaactt tacggtgttc agttaaaata atttctaaag cagatactac
    12301 cgataaaata tcgaaaggac taattttccc catatgacca attcttaaaa tttgaccttt
    12361 aagatggcct tgaccaccag caattgttat tttaaagcga tttttaagtt catctttgat
    12421 tattttcact tcatcatttg tattaggttt gaatgctgta accgttggag atgcatcttt
    12481 atcattgact aataaagtta aatctaatgc ttttaaggcg cttcttaatg catttctaat
    12541 tgcatagtgt cgtgctatta cgtgattgaa accttctgct tttacggttt caacgtatgc
    12601 atttacacct ctaaataaag acacatttgg tgtgaacggt gtagaattgt cagcttgtga
    12661 cgaaatgtat ttatttaagt ctagataaaa ttttggcgta gttacttctt tgaaatgttc
    12721 ttttgcacgg tggctataag ctacaaaagc taatcctgga ggtaacataa ttgctttttg
    12781 actaccagaa acaagtacat caattttatc tttgttaatg tcaacatcaa cagcaccaat
    12841 gcaacttacg ccgtcaacta caaaataaat attactatta aattgattaa tggcatttcc
    12901 taactcgtga atagggtgta gcactgtcgt agatgtttcg caatattgac taaatactgc
    12961 tttaacttca acatttaaag ttgaaaggaa attgatgaaa tctttgacat ctacagcttc
    13021 tccccatgtt acgtcataaa tatgcacatt tttgtaataa gtttgtgcaa tttgtttaaa
    13081 tcggttacca aaggcacctg aaacaatgac aacgaagtga tcttcagggt ttacaatgtt
    13141 caacatacta gcctccaaga cgcttgtacc gctagatgtt aaaataagta catcattttg
    13201 actcccaaat attggcttta gaccttgaaa tgcttgttgt gcgatgtctt caaaatcttt
    13261 agaacgatga ccaaccatag gtgcttgaat ttctctcata atggcatcag gtacaggggt
    13321 tgggccaggt gttaataaca acggttgatg ataatacatt tatatgcctc ctataaataa
    13381 gatttcacta ttttagcaaa ttttctgaaa atttaaaagc ctaaaaaagt gataacgcta
    13441 tattgtcgaa caaacgttat cacttaacga ttttatttgg cctgaatgat gggatagatt
    13501 tttatgtcca cattatttct tacagcattt gaaatcatgc aattattatc tgcaattgtt
    13561 atcaattttg gtaatcgctt ttctaattgt gctatttgat cacttggaat ttgaatttga
    13621 ggatggtgca caatttttga catactgaat tttccgttat ttaaacaagc tgttccaatc
    13681 gattgttgtt caattgaaat atctgtgaac tttgcacgtt caagagtagc tgctaatgag
    13741 atgatataac atgatgaagc ggctgatact aacatttcat cgggatttgt tcctatacca
    13801 acaccaccta aagaagcagg tatagaaata ttctctgaaa gtatgtcgcc ttgaacgttt
    13861 ccgacattgt tacgaccacc ttgccaagaa gtttggactt taaagtcatg ttgatgcaat
    13921 tgctttaacc tccaatataa ttgtgatagt ttaattttag aatagtttat caaagtttaa
    13981 cagaaaggtg acttatcaat gactctgaat aaactgaaag atgaattaca aattgtttcg
    14041 caccgtggat tgccgagtga ttttcctgaa aatacaatgg tcggttatcg agaggtaatg
    14101 gggctcaatg ttgctatgtt agaaatagat gttcatttga ccaaagacca acattttgtt
    14161 gtgatacatg atgaaacaat tgatagaaca tcggatggta aggggcgtat tgctgattac
    14221 acattatcgc aattaaaatc atttgatttt ggtagttata aagatgttgc ttttaaggga
    14281 gaacgaatac ccacgttaga tgaagtactt tcattatgct taaagtatga taaaaagcta
    14341 ttgattgaat tgaaaagtcc gaatttatat ccagaaatag aatgtaaatt gctggcattt
    14401 ttggaagaaa agaaagtcga tgcgacacaa gtagttatac aatcttttga tatcgaatgc
    14461 atcgaaaagt taaacacatt gggcagtata tatgaattag gtgtgctttg tagtaaacgt
    14521 aattattggt ataaaaagcc aaacttttca agaattgctc aaattgctag ttatgttaat
    14581 cctaattatg cattagtgac cagaaaattt gttgataaag cacatcatca tcaattacag
    14641 gttatgccat acacagttaa taaattgaaa actggagaaa aattaagaca aatgggtgta
    14701 gatggattaa ttacagataa tccgaaatta tttattaagt gagacgatga atggtaattt
    14761 gatagaggga tgaggattcg tatatcgcaa ttgataattt taaaaactaa tgatgaaata
    14821 ctaaagataa ttaaacaaat aagacttgta aattctaagt tgattgtaaa atagggtagc
    14881 tccatataat tttgaatcta tcatttttaa attaataaaa aaaacaccca caattgtggg
    14941 tgtttgtatt gaacgtatta tcttgagtag tactcaacga ttaattgttc gttaatttca
    15001 gcaggtaatt cgctacgttc tggtaaacgt acgaaagtac cagttaagct gtcagcatca
    15061 aagtttaagt actcaggtac gaaattgttg atttcaactg attcaacgat gatgtttaat
    15121 ttttgagatt tttcacgaac tgaaattgtt tgaccaggtt taacagaata agatggaata
    15181 tcaacacgtt taccatctac taagatatga ccgtggttaa ctaattgacg tgcttgacga
    15241 cgagtacgag ctaaacctaa tgaataaaca acagcgtcta aacgacttgc taataagatc
    15301 atgaagtttt caccgtgtac accgaatttt ttaccagcga tgtcaaatgt gttacggaat
    15361 tgtctttcag tcattccata taagtaacgt aatttttgtt tttcacgtaa ttgtaaacca
    15421 tattctgata attttttacg ttggtttgga ccatgttgtc ctggtgcgta aggacgtttt
    15481 tctaattctt taccagtacc gcttaaagag atacctaaac gacgagattt tttccagttt
    15541 gaacctctga atcgagccat aataagactc ctcctttaat tcttttttgt tgttatgaat
    15601 aaacaaaaaa gagtgttgca tgcttactaa gatatattgt tttatgtgtc ttcaccttat
    15661 agcgacagtt acgtgacacg tccgcattgg gaacaacata gcgctcaata atatacaact
    15721 gctactttcg ttaattcaca caaagattat tgtaacattt tcctgctaac tgtcaacact
    15781 ttattgaaaa tgctgatgtc cttttatgcg agttgctttt caataatttt aacaattgct
    15841 tcaagatgtt ctttgtcatt gtcatcaaat cgatccgtta taggggcatc gatatctaag
    15901 acgccgataa ttttatcatc tttgaaaatt ggaacgacaa tctcagactt actattagca
    15961 tcacaagcga tatgtccttc gaattgatga acatcagcta caatttgtgt acgacgttct
    16021 gaaacggctg taccacatac accttttcca attggaatgt ggacacaagc ggggtgtcct
    16081 tggaaaggtc caagtataag ttcattttgt tccaataagt aaaagccaac ccaattaatt
    16141 tgatctagat tatcatttaa taaggcagac atgttactta aaatagcaat catgtgatgt
    16201 tcatcttcaa taaggcttgc tgcttgtttc tttaataatg tgtagtttgt tgggttaatt
    16261 gttgtcatat ttaaaacctc t
//
LOCUS       DAMQYT010000039        16111 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMQYT010000039 DAMQYT010000000
VERSION     DAMQYT010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16111
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.039"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..953)
                     /locus_tag="PGA33_000991"
     CDS             complement(<1..953)
                     /locus_tag="PGA33_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HDG6472818.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNP"
     gene            complement(1303..1665)
                     /locus_tag="PGA33_000992"
     CDS             complement(1303..1665)
                     /locus_tag="PGA33_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDG6472819.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
     gene            complement(1662..1832)
                     /gene="mazE"
                     /locus_tag="PGA33_000993"
     CDS             complement(1662..1832)
                     /gene="mazE"
                     /locus_tag="PGA33_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDG6472820.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            complement(1917..3065)
                     /gene="alr"
                     /locus_tag="PGA33_000994"
     CDS             complement(1917..3065)
                     /gene="alr"
                     /locus_tag="PGA33_000994"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDG6472821.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            complement(3131..3490)
                     /locus_tag="PGA33_000995"
     CDS             complement(3131..3490)
                     /locus_tag="PGA33_000995"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDG6472822.1"
                     /translation="MIHGIGVDLIEIDRIQALYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            complement(3494..3985)
                     /locus_tag="PGA33_000996"
     CDS             complement(3494..3985)
                     /locus_tag="PGA33_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDG6472823.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLIAFNILNWLFWEI
                     LDNHLSIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            complement(3972..5555)
                     /locus_tag="PGA33_000997"
     CDS             complement(3972..5555)
                     /locus_tag="PGA33_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDG6472824.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            complement(5548..6027)
                     /locus_tag="PGA33_000998"
     CDS             complement(5548..6027)
                     /locus_tag="PGA33_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472825.1"
                     /translation="MSDYTKLTKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLFIKPLWQYHCRFYKVDQLSVQYRTSFFIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEERTMSNLRGVESDV"
     gene            complement(6235..6795)
                     /locus_tag="PGA33_000999"
     CDS             complement(6235..6795)
                     /locus_tag="PGA33_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDG6472826.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RMKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHVSTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            complement(6815..8842)
                     /gene="kdpB"
                     /locus_tag="PGA33_001000"
     CDS             complement(6815..8842)
                     /gene="kdpB"
                     /locus_tag="PGA33_001000"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDG6472827.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNLKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
     gene            complement(8861..10537)
                     /gene="kdpA"
                     /locus_tag="PGA33_001001"
     CDS             complement(8861..10537)
                     /gene="kdpA"
                     /locus_tag="PGA33_001001"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HDG6472828.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPIGGLGPMVLMMLNVVFGGEGVGLMNLLIFVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            complement(10561..10671)
                     /gene="kdpF"
                     /locus_tag="PGA33_001002"
     CDS             complement(10561..10671)
                     /gene="kdpF"
                     /locus_tag="PGA33_001002"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDG6472829.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            10809..13466
                     /locus_tag="PGA33_001003"
     CDS             10809..13466
                     /locus_tag="PGA33_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDG6472830.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVNLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SYEQIEFAVIDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIYTDFNEGDV"
     gene            13466..14161
                     /locus_tag="PGA33_001004"
     CDS             13466..14161
                     /locus_tag="PGA33_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDG6472831.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(14530..16050)
                     /locus_tag="PGA33_001005"
     CDS             complement(14530..16050)
                     /locus_tag="PGA33_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDG6472832.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
BASE COUNT         5352 a         2999 c         2295 g         5465 t
ORIGIN      
        1 gggttttgaa gctttaaaat tgcttcatag ataatttgaa caatatcttg tgggtgcata
       61 tgtttatgtt ttttaatata ttctaaaagt ttttgtttat ctataaaggt accttcacta
      121 tttctagctt cagtcacacc atccgttaaa atgataatta aatcatcaag gtatattgga
      181 atttcttgtt gttgatatcg tgtttgtgaa ctgattccta acactctacc tctaactgaa
      241 atttcttcaa attcttcttt ttcagcgcga taaatatatc caggctcatg accagctgaa
      301 ctacaataca ataaatggtt catttcttca tataaaccat aaaacattgt gacgaacata
      361 ttttgattaa tattcttttc aacaacacga tttaaacgtt ttaaaccatc actcggtaat
      421 tgtgagtgtc cataagaatc catgccaaac tttatcatac tcattgctaa agcagctggt
      481 ataccttttc caatgacatc tgcaacagca aagctcattg tgccatcgtt atggtcaatt
      541 aaattaaaat aatctccact tactttttgt gccgccactg aaataacgcc aatttgaata
      601 ctatcaaatt gtggaatatc tgttttaagc attgtttgtt gtaagctaga agctaagtct
      661 atctctttat cgtgaacttg aagtttatct accaatcttt gataatctcg ataactataa
      721 ccaaagcctt taacgatttc ttgtaagaca tctaatgtct tgaacacatc ttcacgcgtt
      781 aagtttaacg tcaatatata gtttttgtga atatcgacaa tgtcttcagg caagacgtcc
      841 ttacgaatca cttcgtcagt gtatttctca cactttttta tcaattctac tttatcttgg
      901 cacgttaaac tttcatcaat taaaccctta taatgttgct taaattcttc cacgattagt
      961 tgcctcctca cttcccttac aaagcaaatg cttaagcaag gataattatg attcacttac
     1021 taacatttat ttaattgcac ttatcaattt tgtcgtttgc aattttgctg tagatgatac
     1081 ttttaagcta aaaaaaactt taggatggaa tacctaaagc ctaaaaaact taagacatcc
     1141 ttttataata aaaaatatga atatcataac tggtatttgc taaaatcttt gtttataaga
     1201 aaattcttcg tacagaattt agaaatagtt ataattatta taaagttatt gtcttttata
     1261 tcaatatttt atttatctct gaaaaataca tataatagac gcctaatttt tctggtgagc
     1321 tactgcattc agccctaaac taatcattag tgcattatct acttctttca ttttatcatc
     1381 ggataagtac gtcagttttt ctttcaatcg ttttttatca agtgtacgaa tttgttctaa
     1441 taatataact gagtctttat ccaacttata ctttttcttt tcaatctcta catgtgtcgg
     1501 tattttcgct ttattaatcc taccagttat tgccgcaaca ataactgtag gactatattt
     1561 attaccagta tcattttgaa ttatgactac aggtctgact cccccttgtt cagatccctg
     1621 tactggtgat aaatctgcta aataaacatc tcctcgtcta atcattcatt cgttgaatta
     1681 gaagataaat atgtttcgtt gcaatcgcat gcttcacact ctatcggaaa agcttcgttc
     1741 gctagggaga gatttaaatc agccatttgt gaatatcctt cttttaaaga ttgttctaag
     1801 ctatgacttc tattttgact aaaagataac atatgagaac ctccaattga ttccaaaagt
     1861 tattaaatga cctttaatta ctctaatgat aacaaatttt atatgcactg accatattat
     1921 tttaacaatt catttgttac aaaacgttga tcaccatcat gatagattcg cggcaaacgt
     1981 ctcgacaagt tacaaagcac ttcataatta atagtatgtt gcttttcagc taccacctct
     2041 accgactgtg gactttctct atgattatct attaaaatca ccgaatctcc agctttaact
     2101 tgatctggca ctttaacaat tgtctgatcc atacatacgc gaccaataac ttcgcattga
     2161 tgaccattta catttacaaa gctaccttgc attatgcgta aatagccatc tgcatatcca
     2221 ataggtaaca atgctattgt agttgggtca gtagctgtat aagttgcacc ataacttaca
     2281 gactcacccg cttgtagcgt ctttgtttga actacattag caattaattg cacacttggt
     2341 ttaaggtgta ctttaacttt ttgctgtaca tactctgatg gataatatcc ataaagggaa
     2401 attcctggtc ttattgcatt acagaattgg caatccatta atagagagcc tgctgagttc
     2461 tgacaatgta tatattcagg tttaattgct tcattgacca tatctttaaa acgttgatat
     2521 tgttcagttg tcatatctcc tggttcgtca gcacaggcaa agtgtgtaaa cacgccttca
     2581 aatacaagtt gctcatattg ttgaatgatt tcaatcactt cttgatacgt tttagtatct
     2641 ttaataccta aacgtcccat tcctgtatct aatttaatgt gcaaccataa ctttttctct
     2701 tgctcaccag aaatgttttt aattgcttct ttcaaccact gtttagacgg aaccgttaag
     2761 gcaactcggt gttgtatcgc tttatcaata tctttagctg gtaacacacc taagactaaa
     2821 attttagcag taatcccatg cattctaagt tctatcgctt catctaacgt tgctacagca
     2881 aaaaatgtgg cgccattttc cattaaatga cgtgctactt taacactacc tagtccatag
     2941 gcattggctt taacgacagc catcactgtt ttatttggat gcaatgtact gaatactttg
     3001 aaatttgatg caacagcgtt taaatctaca ttcatatacg cagatctata atatttatcc
     3061 gacatattac ttcctcctgt aattcccaca cgttttaaaa ctagatctta attatcattg
     3121 tataacaaat ttaaaatgct gacttttcta aaacaacttg gctcatcgca taatgctcag
     3181 tgtgtgagat actaacatgt acgataaacc cttcgtaatc aatctttggt ttgccaagtt
     3241 cgtcgttgta acagtctata tcgttaaaag ctacatgttt tcctaagcct gtgcctaatg
     3301 ctttactgaa cgcttctttt gtagcaaacc tgccagctaa aaattcaatt ttacgttgct
     3361 catgtgtgaa attgttgaat ttgtgctgtt catttttagt taaaatccgc tcaaccaatt
     3421 ttggttgctt actatataac gcttgtattc gatcgatttc gattaaatct acaccaattc
     3481 catgtatcat tgattattcc tcatttttat tgttttctaa atgtctctcg ttatttaacc
     3541 atcgtttcaa tctacgtgct tcacttctat ctattaattc aatttcagct tgaccacccg
     3601 ctgtataaag ggtcaaagtt gctaatcgat agcgtctcat tataggacca acatcgatat
     3661 caatattttg aatacgaaaa tatggtatta ccttttcatc caaaaataaa atgccgtttc
     3721 gtacacgcaa atggtgtttt tcaaatgcat atctgcagtg cttatatcga tagactggcg
     3781 ctataacaag cgtgaaaaca gcaacaagta atattataat cacactacta acaatggata
     3841 aatggttatc taaaatctcc caaaatagcc agttcaaaat attaaatgcg attaaaagta
     3901 caagcgctat gggtatccaa aacagcacag cacttaacct catcactttt ttagcgtgtg
     3961 gtgacataaa attataatcc ctcatcattt tcacctctta aataccatga tttcaattta
     4021 tttgcatctt cacttcttgc gtattttaag ttaatcgatt gggctcccac acctttagca
     4081 acaataaagc taaaattatt taaattgttg cgtttaagta atgtatgttg ccaagtgtca
     4141 aatcctataa tgtgatgcgc tttaaaataa taaatatttc gtttcaatag ctcgaaattc
     4201 tggatagtaa tttgttcttc tgtcatttta aaacccgcat gtttgacata aagatatcct
     4261 ttgatcacaa ataaaccaat aatgactatt gttataatcg taaataacaa taataattga
     4321 ttccaaaaat aacagcctat acctgccata gctatgacaa taatactagg tattaaaaag
     4381 tgtctgtgga aacctgacaa aggcatacct tcattaactt gttgataaga taaatctggt
     4441 actaaattct ggataatttg atatgctttg tctcgtttaa taaacggcaa tatcggcaca
     4501 ctacctgaat cattgtcatc atcttcgatt tttttatttt caaaatcact tgtgatgata
     4561 aaatgtattg ctgtgtagcc aaataatctt cgtatatatg actgacgttc aacaacagct
     4621 tgcaatcgtt tcgttggtac agttactttc ttcttgttaa agagaccata ttcaatttta
     4681 agttgctcat tttcaagcat caccgaaaag ccataaaatc ttatcattgt tataatcgtt
     4741 ccaataatat atgccactat taatactagt aaaatgatga ttaatactga aatacttaca
     4801 atttgaaccc attgactaat ttcatgattt agcttcgacc atgggatcaa ctctcttaca
     4861 gccccgtaaa tcggtactaa agctgctaac gttacaccaa tggcgccact ggtcattgcc
     4921 ataaatagtg attctttaaa attcatctga tatataggaa tgcgtttatt tttctgatta
     4981 agcatactat cagtgttctg cacttcatct aagcgacctt ctgcgatgtc ttccacatta
     5041 ccttcaatgt catgattaca gttgtcattc ttctcagcac tagacttttg cgccacttct
     5101 gtcttcaact ctgtttgcaa ttgatcaata tatcgttcaa gatattcacc ttgttttttc
     5161 gaaataacac ttaagacaat accatcactt ggtgttttga tttgtaaatc cacaccacca
     5221 ataatttgat tgacgacccc ttgtgtcata tcaacggact gaatacgttt tatatttaat
     5281 tctttacgct ttttattaaa aatacctgtt gttaaaatga aataattatc ctcaatccaa
     5341 tatcgcgtgt tcataattcc gacaatttga gaaatgtatg atattaaaaa gaatacaaat
     5401 acaataccta tccataaata tgattcggga ttcgtataat caaaatcttt caattgaaag
     5461 ataatgaaaa taaaaaagac gactatgttt tgtttgatag cattgattat gccattaaaa
     5521 tatgaaatcg gatgtaattt ttgaggttca gacatcactt tcaacccctc tcaaattcga
     5581 catagttctc tcttcgatta ttttaacatc gtcatgagac atcatcggta aataaatagt
     5641 atgacctgca gtcataaatc caactttata caaattaagc actttactaa ttggattaga
     5701 tttaatcgac aagtattgta aacgttcaat tcgactcgtt tcttctttat atataaaaaa
     5761 tgatgtacga tattgtacac ttagttgatc aactttataa aagcgacaat gatattgcca
     5821 taaaggctta ataaataatt ttaatgtact cagagcacct aaaaccaaca aaatataaag
     5881 taagtaatgt ggccattcaa atcttaacca tataaaataa aaaatgacat acacagctac
     5941 actcaatata aattctaagc cattcgtaat gtagtaatac aacaatgctg acttaggact
     6001 cttagtcaac ttagtataat ctgacatata cccctctccc caaataaaaa attatacgga
     6061 tttataatct atttcatttt atttttatat gatgataatt atagcatatg gaatatttca
     6121 tgctaattta ttcttcctaa aggtacatct aaaaatttaa ttaagcagaa agtgcttgaa
     6181 ttgctaaaaa gacaccatgt ataattttat caacatgatg cctttcatct ataatcaatc
     6241 tttcatctta tcaagagcga tatttagttc aagcacattc acataatcat ttgttaacac
     6301 accacgctgc ttacgatgtt gaatcaagtc ggccactctt gaagtagata catgacgagc
     6361 atcagcaata cgaggtgctt gcttcaatgc attttcgacc gtaatatgcg gatctaagcc
     6421 cgacccagaa cttgttgcag catctattgt tacatttgaa ttcccaaatt taacatgatg
     6481 tttcatgcgt gctattaatt cggtgtttcc attcgattca ttacttccac ctgaagatac
     6541 gccgttttta tataattttt caggattcat attataatca actgcactcg gtctcccgtg
     6601 aaaatatcgt gtctctgtcc agtgctgtcc aatcaatttt gatccaacta tacgattgtc
     6661 atacgtaatt aaactgccat ttgcttgttg ataaaaaaat atttgaccaa ttaacgtgat
     6721 agctaacggg aataaaaatc cacataatac catagttatt atcgttaaac aaatactatt
     6781 tcttatcgta ttcatggtac aggctccttc ctctttacac aaaaaattgt acaatcatat
     6841 ctattaattt aatgcctaaa aacgggacga ttaatccacc taatccataa atcaacatat
     6901 tatttataaa gattctatca atgctgtaac cctttacttt tacacctttc atggcaattg
     6961 gaattaaggc aacaatgatt aatgcattga atatcaaagc tgatataatt gcagatttag
     7021 gtgacgataa atgcataata tttagtgaag tcatttctgg aattgttgac atcattagtg
     7081 caggtaaaat tgcaaagtat tttgctacgt cattagccaa actaaatgtc gttaatgcac
     7141 ctctcgtcat taataattgt ttgcctattt ttacaacctc tattaacttt gtaggattcg
     7201 aatctaaatc aattagatta gctgcctctt tagcactaat tgtccctgag ttcatagcta
     7261 atcctatatt cgcttgtgct agcgcaggtg catcatttgt accatctcct gtcatcgcaa
     7321 caatatggcc tttcgcttgt tcatctttga tgactttaat tttatcttcg ggtttacact
     7381 ctgcaacaaa tctatcaacc ccggcttctt ttgcaattgt agctgctgtt aaagcattat
     7441 cacctgtaca cataactgtt tcaatcccca tttttctcaa ttcagtaaat cgttctacaa
     7501 gaccatcttt aatcacatct tttaaataaa tcacgccaag catgacattg ttttcaatga
     7561 ctattaatgg tgtgccacct ttactcgata catccataca gagagactca atattaagag
     7621 gaatattgcc ttgttgttgt ttgacaagat ttatcatact attaggtgca cctttgaata
     7681 ccgatatttc atttgtaatg attccgctca ttctagtttc agctgtaaaa ggcttatatg
     7741 tgccatcaat gtctttaggc agctcattta tatacatctg cttcgctaat cgtacaatac
     7801 tttttccttc tggcgtatca tcgtagattg atgacatata agcagcgact atcaattttt
     7861 caagcatttg ttgattcact ggtaaaaatt cactagcgat tcgattgcca taagtgattg
     7921 tgcctgtctt gtctaaaatc attacatcga catctccaca tacttctaca gcacgcccac
     7981 ttttcgctaa tacattgaat tgagtaacac gatccatgcc tgcaatacca atcgccgata
     8041 acaaaccacc gattgtcgtt ggtattaaac atactgttaa cgcaatgagc atcgcaatag
     8101 gtaaaattaa atgcaggtaa gatgctattg gatataacgt tacaataacg actaaaaata
     8161 taattgttaa cgttgttaat aatgtaaaaa gtgcaatttc atttggtgtt ttatttcttt
     8221 ccgccccttc aactaaggca atcattttat ctaaaaaaga tgtacccgct tcactctcaa
     8281 cacgtatttc taaccaatca gatgttacaa gtgtaccgcc aatgactcca tcaaaatcgc
     8341 cacctgattc ttttatcaca ggtgcagact caccagtaat tgcagattca tcaacggttg
     8401 ctaatccatt tattacaacg ccatcagcag ggattgtttc tccattttct acccgaatat
     8461 tttgtccggc ttttaactct gtggcgttca ctatccgata cgcaccattt tcttctatca
     8521 atcgagcagt taaatttgat tgtgcttgtc ttaaactatc agcttgcgct tttccacgac
     8581 cttcagcaaa ggcttctgaa aaattagcaa acaatatagt tattaataat atgataaaaa
     8641 ttgtaatcaa ataacctcgc gatagatagc tagttccaaa tatgtcagga aaacatatta
     8701 atatcaacgt taaaatcatt ccaacctcaa cgacaaacat tatcggattt tttattaatt
     8761 gtttaagatt cagcttataa aaactcattt tcaaagcttc tatcaccatt gtttgattga
     8821 aatatttatt tacatgatgc attttttcca tccctttact ttattttaaa gttaaaaatt
     8881 caccaatagg accaagtaat agtactggaa taaatgtcaa accacttagt aaaacgataa
     8941 atacgattag tgatacgcca aaataaggtt tatcaatcgc tattgtatat ttatcttgat
     9001 ggtatgattt tttattcact aaacttgatg caatcattaa ttgcaaaata attggtatat
     9061 aacgagaaag caacataatg attcctgtag agatattcca gaatgttgta tcatctttca
     9121 gtccttcaaa ccctgatcca ttgttcgcag cagctgatgt catttcatac ataacttgtg
     9181 aaataccatg aaaagacgga ttcgttatac tttcacttgc tccaggaatc ataaaagcaa
     9241 gtgctgaaaa tactaaaatt aaaattgggt gtatgagaaa gactaagaca atacatttca
     9301 tttcacgggc gccaattggc atatttaaat attctggtgt tttaccaacc atcaaactgc
     9361 atataaacac cgtcagtaag acaaatatca ataaattcat gagtcctacg ccttcgccac
     9421 caaatacaac atttagcatc attaatacca ttggtcctaa tccacctata ggcgttaagc
     9481 tatcatgcat gttattaaca gaacccgttg taaatgccgt cgtaataact gtaaatagtg
     9541 ctgacaaacc tgctccaaac cgtacctctt taccttccat attcggtcca taaatgccta
     9601 aattcgctag tattggatta ccacgatact cactccacat agttaatgta agaattgcta
     9661 taaaaatgaa aaacattgcg acaaataata tcaacgcatg acgatgtact cgtttaccat
     9721 gtctacttaa catgcgacca aataagaaca acattgacat aggaagtaac atcatactgc
     9781 ccatttctat aaaattgctc caaatatttg gattttcaaa aggtgtcgca gaatttcctg
     9841 ctaaaaatcc tccaccattc gtaccaagat gttttattga ttcaagtgat gcaataggtc
     9901 caaatgcaat atgttgaata tgtccgctta aagtccgaat cattaaatta gcatgcaacg
     9961 tttgtggtac accttgagtc atcaataaaa tactaattaa acatgataat ggtaaaagta
    10021 ctcggacaat aaaccgaaca atatcttgat aaaaattacc aatgatatta gttaatccag
    10081 ttaaacgtct caacatcgct atacaaacgg cgtaacctga tgcactagat gtaaacatta
    10141 aatatgtcat tacaatcatt tgcgttaaat atgtcacatc tgattcaccg ttatagtgtt
    10201 gtaaattact atttgttaaa aaagatattg ctgtattaaa cgctaaatct atcgattggt
    10261 ttaaattatg atttggattt aaaaaaagcc attgctgaac tattagcaat acaaatgtta
    10321 taaaccccat aaatccatta aatgccagaa aatgtttgac atatgtttta gctgacatgt
    10381 gttctaaatc tgtgccgata attttaaaac acatattttc aaatctagta aatattaaat
    10441 ctactcttga cgattgcacc aatgctacgc gatatagata tccactaaaa acatacgtaa
    10501 tcataaccat cattgttaga aacaaaatta tttccatgat aaccctcact taatatattt
    10561 ctaaaatttt tcactacgaa ttaaggcata aaataaatac aaaactaatg caataactac
    10621 cagtaataaa acgatgagca ttgccataac ctccttacaa cacaacaaca tcgtaacaac
    10681 ttgtttatga gagaaatatt aattttcaaa cttagttatt aagaaatcat taagatgtgt
    10741 atgcagaaat aaattttata gcatttaatt gtgaagaata ttatgatatt gctatcgagg
    10801 tgaaggttat gtcaaacact gaatcgctaa acataggaaa aaagcgtgga tctttaacaa
    10861 tttacatcgg ttacagtcca ggtgttggta aaacatttga gatgctttca aatgccattg
    10921 aactatttca aagtaacgtt gatattaaaa taggatatat tgaaccgcat cagcgtgatg
    10981 aaacaaatgc attagctgaa caattaccta aaatcacaac caattttact aaacatggta
    11041 gtcatcattt tcaatattta gatgtcgacc gcataatcga agaatcgccg acaatagtac
    11101 ttatcgatga gttagcacat acgaatattt ctagagatcg tcatgagaaa cgatatatgg
    11161 atattgaaga aattttaaat catggtatcg atgttcatac cactttgaac attcaacata
    11221 ttgaaagttt aagtagtcaa attgaactga tgaccggtgt acatgttaaa gaacgtgtac
    11281 ccgactattt cataatgagc gccgatgtat tagaagtcgt agatatctca cctgaacaat
    11341 taattaaacg cttaaaagct ggcaaggtat ataaaaagga taggctagat gtagcattta
    11401 gtaatttctt tacgtatgcc cacctaagcg aactgcgtac attgacgtta agaacagttg
    11461 ccgacttgat gagtgataaa gaaaaagtcc gacacaacca taaaacgtca ctcaaacctc
    11521 atattgctgt ggcaattagt gggagcattt ataatgaagc agtaattaaa gaggcattcc
    11581 atattgctca aaaagaacat gcgaagttca ctgctattta tatagatgta ttcgaaaaaa
    11641 acaggcaata taaagatagt caaaagcaag tgcatcaaca tctcatgctt gcaaaatcat
    11701 taggagcaaa agtaaaagta gtttatagcc aaaccgttgc attaggatta gacgaatggt
    11761 gtaaaaatca agatgtaacc aaattaatta tcggacaaca tattagaaat aagtggcgag
    11821 actttttcaa tacaccttta attgaccatt taatgtcctt tgaacatagc tataaaatcg
    11881 aaatcgttcc aatcaaacaa atacctgttg aattgaaaat gaacaaatca ccctatcgtc
    11941 ctaaaggcaa acgtttcgcc atagatatgt taaaaatgat tttgattcaa ataatttgtg
    12001 taatgatggg actgtggatt tatcaacttg ataagcatga gtctagtacg attattttaa
    12061 tgatttttct catcggcatc attttattat ccatttggac gcggtccttc atcattggct
    12121 ttttagcagc aattattaac gtatttgttt ttaattattt ttttacggaa cctagatata
    12181 catttgaagt atatcgcttt gactatccta ttacatttat cgttagcatt ttaacaagta
    12241 ttttaacgag tgctttatta aaacaaatta aattccaata ctccattact aaaaagcaac
    12301 tttatcgtac agatttatta tttcaattta atgattcgat taaacaaact tatacagttg
    12361 aaaacttact aataaatgct ggataccaaa ttaatcaatt attgcaacaa tcaattacta
    12421 tatacgttat caatcaatcg aaagtaatta aaacaatacc attgcaaaac catatcgata
    12481 atacgactca acagcatgaa caagcattaa gctgggtaat taaaaatgaa agacaagctg
    12541 gtgcaacaac agatacattt ccaggcatta ataaatggtt gataccaatt ggcacatctc
    12601 cgattaaagg cattctagcc attgattacc aaagttctca agtgattaat ccatatgatg
    12661 catcaatatt agaatcaatg ttaaatgaac tttctctcgc cgttgaaaat gtgacgttac
    12721 ttaaacaaac gagagaatcc atgttacagg ctgaacgtca actgacacat tcaaattttt
    12781 taagatcaat ttcgcatgac atacgtacac cgttaacaac aattatggga aatttagata
    12841 ttttagtatc acatagtaaa gatatgtcta tcatagaaaa ggaacaatta cttgtacata
    12901 gttttcaaga aagtcagtat ttatatctat tagtgacaaa tattttatca ttaacaaaat
    12961 tacagtcatc aaatgttcag ataaaattgc aaccttatct cgttagtgaa ttagtagaag
    13021 aaatcgatat gattttagaa cgtcgccatt taaaaaagcg tataactgta tcatcctcag
    13081 taaatttaca gtttatacac attgacagta agttgatttt acaagcttta ttcaatttga
    13141 ttgaaaatgc agttaaacac acttctactg acaccaaaat caatttatct attcgttatg
    13201 ctagctatga acaaatcgaa tttgccgtta tagacgaagg acctggaatt agtttagaag
    13261 aacaacaaaa gatattcgag cctttttaca caggatcaaa taaatatttt aaagataacc
    13321 aaaaagaaag catgggacta ggcttatatt tagtacagac tatcctacac aaacatcaat
    13381 caaacttaca atataaaccg aaccaaccac acggcagtat attctatttc aatatatata
    13441 cagactttaa cgaaggagac gtataatgca atctaaaata ttgataattg aagatgatca
    13501 cgcaatcaca catttacttg atgttgcatt aactttagat tattacaatg taactacagc
    13561 cgacaatgcc acacaagcac actttaaaat tcaaattgat aaaccagatg tcattttatt
    13621 agatttaggt ttaccagata aagatggatt atgtttgatt tcagaaatca ggcaacatac
    13681 tgacattcct atcattgtaa taagtgcaag acaagaagaa caaacaatta ttcaagcttt
    13741 agataacggt gcgaatgact atatgactaa accttttaat gttgatgagc ttcgggcacg
    13801 aatacgagtc atcgagagaa tcgctaagtc acatcaagaa actaacattg tatttactaa
    13861 cggtttgcta agcatcgact ttggctccaa atctgttgtt attaataatc aggaggtaca
    13921 tttgacgcca aatgaattta gcttgttaga actattgtca aatcataaag gaaaagtact
    13981 cacttatgag atgatattaa aacgaatata tggctatgtt aataagactg aaatgcctag
    14041 tttaagagtg catatgacat cattaagaca aaaactttca caatgtcatg aggacgcaaa
    14101 agatattatt aaaacacatc cacgcattgg ttatcaaatg ttgcagtgga aagagaaata
    14161 attaaataaa aaagatcgct gccattaaac gaatgattgg cagtgatctt ttttatattc
    14221 aacttattat ttatcgcgca ctagcgtcag caaaaagccc tattgttttt tataagaaca
    14281 tgatactgaa atttcataaa gtttatttat gttcgattat tcttaattta aaaatagttc
    14341 tttaaattat atactcacca catttggtgg agaaccatat ttacacagaa tgatataacc
    14401 gcatacagat ctcaatccaa ttaaatattt aacctttaaa acgttagtac tttgatatct
    14461 acagagaatc aattattgaa aacaaaaaag agctcaagac attacatctt taagctctta
    14521 atctataaat tatttttgat ggtcagcaaa tgtgcgacct ttcataggtc tgcttccacc
    14581 gctgctcttc tctttacggt cgaatttttt tgtacttttc ttcttacttg agtatccttt
    14641 tgaacgttta ctcttactgt caaatttagg attaccacgc ttactatttc tgttacgaga
    14701 accacttggt ttaccgttac ggcctttgcg agataatggt ttttcaaaag ttaattgaac
    14761 ttcaacttca tcgtttgctt ctactaactc ttgtaaaagt gcagcaacta aatcaacatc
    14821 gttatattca tttaacaact ctgtagaaat gcgtttcaag cgtgattctg actctttaga
    14881 catccagttt tcaacttttt ctttgatgtc atcttcacgt gcttgaagta cttctttacg
    14941 atgtggtgga cgaagtgcac tcatttttct accgtttgca tcttcaattt gtctgatata
    15001 atccatttcg attggattaa caaacgttac agcgatacct tctttaccag cacgacccgt
    15061 acgaccaata cggtgtgtat agctttcagt atcttgaggt atatcaaagt tataaacatg
    15121 actcacacca gaaatatcta gtcctcttgc tgctacatca gtagcgacta aaatattaat
    15181 ttggtcattt ttaaatttct ttaatacttc taaacgtttc gcttgtgtaa tatcaccatg
    15241 taaaccttca gctttatatc ctttagaaat caaagcactt gttaattcat caacacgacg
    15301 ttttgtacgt ccgaatacga ttgctaattc aggttgatga acatctagga aatttgtaaa
    15361 tgtatcaaat ttctctaatt ctttaacaat tgtatagaat tcttcgattt gtggatcaga
    15421 catttcatta ttcattgtct taatgatttt tggtgatttc ataaattgtt gtactaaagc
    15481 ttggattgct ttaggcattg tagctgagaa caacattgtt tgacgttgta ctgctggaat
    15541 tttatccata ataaatctca tatcatcgat gaatcccata ttcatcattt catcagcttc
    15601 atctaaaatc aaagtatgaa ttccgtccgt ttttaatgtg cgacgattta aatggtcgat
    15661 aacacgccca ggtgttccga ctacgatttg tgggcctttt ttcaaggctt taatttggcg
    15721 ttcgataggc ataccaccga atacagtaac aacttggaca ccttgtccac ggctaaattc
    15781 tcttaattgt tcagctacct gcattgccaa ttctcttgta ggtgctaaaa tcaacgattg
    15841 aaccccttgt ttccctacta ctttctcaat taaaggaata ccgaatgctc ctgttttacc
    15901 tgtaccggtt tgagcttgcc caaggatatc aattccttgt aacgcataag ggatactgtc
    15961 tttttggata ggtgtcggct ctttaaatcc cattgattca agtgactgaa ccgtattatc
    16021 cgaaatccct agttctttaa aattttgcaa aataattctc ctttttacct gtttatttct
    16081 caatatactt ttgcatataa gttcattaat a
//
LOCUS       DAMQYT010000040        15859 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMQYT010000040 DAMQYT010000000
VERSION     DAMQYT010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15859
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.040"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..897)
                     /locus_tag="PGA33_001006"
     CDS             complement(<1..897)
                     /locus_tag="PGA33_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HDG6472833.1"
                     /translation="MIPTLEEIIDKYGNLVDYLKFDVTVEVYEDLLLLRSLSDLNEHQ
                     KMDRVSFIKKHLNSNFDYLLDDTEFRSINKIYSNLNIMTHINSQNNNFKRNPFINEEQ
                     LKSLLAIKKVDAHMSYDSNITSKALAEINKTQKRLVTKIDTLYNQSKKENEYVRLGEK
                     ISYTKLENHWKYLYNEIQFYNLNNQLISYVALEQEYAWAFLNELFYLIELYLKAFKGQ
                     KKTDYEFGKKLNEFIQSYVIILLIDIRLPVVRLYIIRDIVDTCEGEKDNYKRITLIEE
                     SIKKYRYVKDQIKRFKNGLEESL"
     gene            1036..1326
                     /locus_tag="PGA33_001007"
     CDS             1036..1326
                     /locus_tag="PGA33_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472834.1"
                     /translation="MNKKSKQQEKLYNFIIAKSFQQPVGSTFTYGELRKKYNVVCSTN
                     DQREVGRRFAYWIKYTPGLPFKIVGTKNGSLLYQKIGINPCNNSTPSKGGDC"
     gene            1326..3113
                     /locus_tag="PGA33_001008"
     CDS             1326..3113
                     /locus_tag="PGA33_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDG6472835.1"
                     /translation="MSYTLFEIGPFALDSFGWKETIPPKKDGDSEKVVRMASPIIVNA
                     RFSDPITGVEKLIITNNNGKKDIFESDILTTRNLPSLIKYGYSINEKYIRSLSYALQL
                     MRDRLPLSELYEGVGILETPFGYLISLDKVLKSIQFNQSSPSYPIVDSAYDLTPKGTF
                     DNWFNMYIDEVKGHLLLELAVIFGISALVTSFLKHKHEIEFAGILFSFTGQSSTGKST
                     AAALAVSVAGNPTKGNETLFRSWNATRNALEGYLSNNYGIPIVFDELSSTTLRDTTGL
                     LYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFNDSAQNDGLRVRTIEINEQF
                     TTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDETNNT
                     GIRMFKRYAVITTSAKILGRVLSTDIDIANIRDYFIDYHTHTVSERSLADKAIDVIIQ
                     FVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKIIANVFKQMLNNHQFQDVNNVVN
                     ALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDVEFASILGLTKDKSLLQNW
                     TPSNDNKAAKELFKSANEGIGPSGVHEDF"
     gene            3347..4696
                     /locus_tag="PGA33_001009"
     CDS             3347..4696
                     /locus_tag="PGA33_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDG6472836.1"
                     /translation="MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQ
                     SGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRNLKNALKLIKLCQTYHVHILSVH
                     DGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
                     NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYC
                     GRVNNQYGQFDNMFPSIVSTSIYEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLT
                     NMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEVCRDFYQNQRIYTKI
                     KSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSING
                     HQIQHIIQNIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA"
     gene            4718..6346
                     /locus_tag="PGA33_001010"
     CDS             4718..6346
                     /locus_tag="PGA33_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDG6472837.1"
                     /translation="MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFE
                     VKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVMVYKTNRLARNTSDLLKIVEDLH
                     KQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
                     LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPF
                     SISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQ
                     MRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNF
                     RNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDI
                     AYKQQQYDEVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNE
                     DKTLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKL
                     NNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI"
     gene            6868..7218
                     /locus_tag="PGA33_001011"
     CDS             6868..7218
                     /locus_tag="PGA33_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472838.1"
                     /translation="MKTITQELKQYITHLFQLSNNEAWECEALEEAAENILPERFIND
                     SPLVHLTLETYTYYNDELHELSIYPFLMYANNQLISVGYLDHFDMDFLYLTDTQNIII
                     DERHLLKQGGQDHE"
     gene            7305..7616
                     /locus_tag="PGA33_001012"
     CDS             7305..7616
                     /locus_tag="PGA33_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDG6472839.1"
                     /translation="MKINRYITRGINESIPLDLQILLWHMVEKKDNQPHTDYLHIFKL
                     QEDENILSITHEQEQPTYKLEYHYTNYEKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            7631..8137
                     /locus_tag="PGA33_001013"
     CDS             7631..8137
                     /locus_tag="PGA33_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDG6472840.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKTWDEKKAACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSERYGAVYLVNLFSNIRTPENLKHIKNPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEIMHPLNPKA
                     RQKWILKS"
     gene            8189..9796
                     /locus_tag="PGA33_001014"
                     /pseudo
     CDS             8189..9796
                     /locus_tag="PGA33_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(9787..10047)
                     /locus_tag="PGA33_001015"
     CDS             complement(9787..10047)
                     /locus_tag="PGA33_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HDG6472841.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(10019..11005)
                     /locus_tag="PGA33_001016"
     CDS             complement(10019..11005)
                     /locus_tag="PGA33_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HDG6472842.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            11102..13111
                     /gene="mecA"
                     /locus_tag="PGA33_001017"
     CDS             11102..13111
                     /gene="mecA"
                     /locus_tag="PGA33_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HDG6472843.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWNHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(13157..13585)
                     /locus_tag="PGA33_001018"
     CDS             complement(13157..13585)
                     /locus_tag="PGA33_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HDG6472844.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(13682..14425)
                     /locus_tag="PGA33_001019"
     CDS             complement(13682..14425)
                     /locus_tag="PGA33_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HDG6472845.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
     gene            complement(15262..15633)
                     /locus_tag="PGA33_001020"
                     /pseudo
     CDS             complement(15262..15633)
                     /locus_tag="PGA33_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016912861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
     gene            15687..>15859
                     /locus_tag="PGA33_001021"
     CDS             15687..>15859
                     /locus_tag="PGA33_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDG6472846.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYTLSYRDISEILRERGVNVHH
                     STVYRWVQEYAPI"
BASE COUNT         5777 a         2662 c         2420 g         5000 t
ORIGIN      
        1 taacgattct tctaatccat ttttaaatct tttaatctga tcttttacgt atctatactt
       61 tttaatagat tcttctatca gtgtaattct tttgtaatta tctttttctc cttcacatgt
      121 atctacaatg tctctaataa tatataatct aacaacaggt aatcgtatat ctataagtaa
      181 aataatgaca tatgattgaa taaattcgtt taattttttc ccaaactcat aatctgtttt
      241 cttttgtcct ttaaatgcct tcaaatacaa ttctatcaaa taaaatagtt catttaaaaa
      301 agcccaagca tattcctgtt ctaatgcaac ataactaatc aattgattat taagattata
      361 aaactgaatc tcattatata aatatttcca atgattctcc aattttgtat acgatatttt
      421 ttctcctaac cgtacatatt cattttcttt ttttgactga ttatagagtg tgtctatctt
      481 tgtaactaat cttttttgcg ttttgtttat ttctgccaat gcttttgaag taatatttga
      541 atcataactc atatgtgcat ctactttctt tatcgcgagt agtgatttca attgttcttc
      601 atttataaat ggattacgtt taaaattatt attttgacta ttgatatgcg tcatgatatt
      661 taaattactg taaatcttat tgatacttct aaactcagta tcatctaata aataatcgaa
      721 atttgagttt aaatgttttt ttataaatga aactctatcc atcttttgat gttcatttaa
      781 atcgcttaaa cttcttaata acaacaaatc ttcataaacc tcaactgtta catcaaattt
      841 caaataatca actaagtttc catacttatc aataatttct tctagcgtag gtatcaatat
      901 ataccccccc tccccatttt ttgttccgcg tctaatcata atgcttcatt tatgcttatt
      961 atagcaatca attgaagata gtttaaccta tatgatattg attgactcct taaaaattat
     1021 tggaggaatt ttaaaatgaa taagaaatct aagcagcaag aaaagcttta taattttata
     1081 attgctaaat cttttcaaca accagttgga agtacgttca cttatggtga attaagaaag
     1141 aaatataatg tggtttgtag cacgaatgat caacgtgaag taggacgtcg ttttgcttac
     1201 tggattaagt acacgccagg attaccattt aaaattgtag gaacaaaaaa tggcagttta
     1261 ttatatcaga aaatagggat aaacccatgc aataatagca cgccttcgaa aggaggtgat
     1321 tgctaatgag ttacacattg tttgaaattg gcccatttgc tttagattcc ttcggttgga
     1381 aagaaacgat accacctaaa aaagatggtg acagtgaaaa agtggttaga atggctagcc
     1441 ctattattgt taatgcaaga ttttctgatc caattactgg tgttgaaaag ttgattatta
     1501 ctaacaacaa tggtaaaaaa gacatttttg agtctgatat cttaacaaca cgaaacttac
     1561 cttctttaat taaatatggc tacagtatta acgagaagta tattagatca ttgagttatg
     1621 ccttgcaatt gatgcgagat agattaccac tttctgaatt atatgagggt gtaggtatac
     1681 tagagacacc ttttggctac ttgatatcgc tagataaagt tctcaaatct attcaattta
     1741 atcagtcctc accttcatac cctattgtag atagtgctta tgacttaact cctaaaggta
     1801 catttgataa ttggtttaac atgtatattg acgaggttaa aggtcatctt cttttagaat
     1861 tggcagtcat atttgggatt tctgccttag tgacaagttt tctgaagcat aaacatgaaa
     1921 tcgaatttgc aggaattctc tttagtttta caggtcaatc ctcaaccggt aagtcaaccg
     1981 cagctgcttt agctgtatca gtcgctggta accctaccaa aggtaatgaa actcttttta
     2041 gaagttggaa tgccacacga aacgcacttg aaggttattt gagtaacaat tatggcattc
     2101 ctatagtatt tgatgagctt tcatctacga cacttagaga taccacgggt ttgttatatt
     2161 ccatcgctga aggtcaaggt cgtcaacgtt caaacgttca tggagaagtt aaaacgccta
     2221 agaattgggg tacttctgtc atttcaacgt cagaatacag tatttttaat gattcagctc
     2281 aaaacgatgg attacgtgta agaaccatcg agattaatga acaattcact acaaatgcga
     2341 ccaatgctga caatattaaa aaagctgtag cactaaacta tggtcatgtt ttaccgttag
     2401 tagctaaata tcttataaat cgtgaagatg aagttattca atggttttac aaggaagtag
     2461 attggtttga agctaaattg aaagatgaaa caaacaatac aggtattcgt atgtttaagc
     2521 gttatgcagt cattacaaca tctgcgaaaa tattaggtcg tgttttatct acagatattg
     2581 atatcgctaa tataagagac tactttattg attatcatac tcatacagtc tctgaacgct
     2641 cattagctga taaagctatt gatgtcatca ttcaatttgt ggcacaaaac agaggtaagt
     2701 tttcagatga aggtgctttg aaaaatatgt ttgagaacta tggattaatc tcattaaaag
     2761 atgaccatat cgaagtaaaa ataattgcga acgtttttaa acagatgcta aacaatcatc
     2821 aatttcaaga tgttaataat gttgtcaacg ccttaagaga taaaggtttc atattagctg
     2881 accgtggacg ccaaacaact aaaagaagtg tcaaagataa tagcggtaaa aaacaatcac
     2941 ttgtatttta ccatctaaaa ttagatgtag agtttgcatc tatcctcgga ttaactaaag
     3001 ataagtcgtt acttcaaaat tggacaccgt caaatgataa caaagctgcg aaagaacttt
     3061 ttaaatccgc taatgaaggg ataggccctt caggagttca cgaagatttc taattgtctt
     3121 tatcatacaa ctgtgaagca tcaaactgaa taatcgacgt attgaagata gaacaaataa
     3181 cggagtgaaa aacatggatg aacttatcga gatattagcc gatttggtaa ttgaagaaat
     3241 caatcaaaca aatgactaat atggacgccc aacatctcat tgatgttggg cttatatatc
     3301 aattcatcca taaatatata ttatcactgt gaaaggagga gccgatatga aacaagtcat
     3361 aggctattta cgtcaaagta cgatgaaaca acaatctctt gcagcacaga aacaagctat
     3421 cgaagcaata gccgaaaaac atcatattca acatatcaac ttttatagcg acaaacaatc
     3481 aggtcgcaaa gataatcgta gtgggtatcg acaaataaca caattaattc aacaaggaca
     3541 gtgtgacata ttatgctgtt atcgtcttaa taggttgcat cgtaatctga aaaatgcatt
     3601 aaaactcatc aaattatgtc aaacgtacca tgttcatatc ttaagcgtac atgatggtta
     3661 ctttgatatg gatcaagctt tcgaccgact caagcttaat atcttcatca gtttagccga
     3721 acttgaatcg gataacattg gagaacaagt cagaaatggg cttcaagaaa aagcaaagca
     3781 aggtcgattg attacaacac atgcaccttt tggttacgaa tatcacaacg gaacattcat
     3841 catcaatcaa aatgagtcac caacggtaaa ggctgtattc aattattaca ttaaaggtca
     3901 tggttataag aaaattgcac agttattaga agaagataac acgtatatca atcgacaacc
     3961 ctatcaagtt cgtaacatta tcatcaatcc taattattgt ggtcgtgtca acaatcaata
     4021 tggccaattc gacaatatgt ttccttctat tgtttccaca agtatatatg agcaagcgca
     4081 gagacttcga ttgcaaaaac aaaccaaaca gacaccttcg gataatcaac tcaaacaaaa
     4141 aatcaaatgc ccatgttgta atgcaacact tacaaatatg accattagaa aaaagaatca
     4201 tacattacgt tactacgtct gtcctaaaaa catgaatgct tcacgctttg tctgtgattt
     4261 taaaggcatc aatgcacaaa cacttgaaga taaagtatta gaagtgtgcc gagactttta
     4321 tcaaaatcaa cgcatctaca caaaaattaa aagtgcgatt gacaaacgca tcaaaagaca
     4381 aagaaacata gaaaaacatc acacattgac tcaagaacaa ctgatagaaa agttggcaca
     4441 aggcatcatt gatgcagaaa cgttcagaga gcaaacacaa tcattacgtc aacaaccgca
     4501 acgcactaca tctatcaatg ggcatcaaat acaacacatc attcaaaata ttattcaaaa
     4561 acgtttcacg ttaaacatat tgtaccccta tattgaaacc attcacatta cgaaagataa
     4621 aaatcttata ggaatctatt tcaaaaatga accactcaat attgtcaatc aaaccatgca
     4681 atcatcgatt gcataaatga agaaaggatg taacataatg caacaactta aaacaaaacg
     4741 tgtcggtatc tatgtacgtg tatcaacaga aatgcaaagc acagaaggtt atagtatcga
     4801 cggacaaatc aatcaaatca aagaatactg tgacttccat cattttgaag ttaaagatat
     4861 atacgctgac cgtggtattt caggtaaatc tatgaacaga cctgagctcc aacgtatgtt
     4921 gaaagatgca aaagaaggca atatcgactg tgttatggta tacaaaacaa accgattagc
     4981 tcgtaataca tctgatcttc tcaaaattgt cgaagattta cacaaacaaa atgtcgaatt
     5041 tttcagtttg tcagagcgta tggaagtcaa tacttcttct ggtaaactca tgttacagat
     5101 acttgcgagt ttctcagaat tcgaacgtaa taacattgtc gagaacgtat ttatgggtca
     5161 aacgagacgt gcccaagaag gctattatca aggcaattta ccactaggtt atgacaaaat
     5221 accagatagt aaacacgagc taatgattaa ccaacatgaa gctaatattg taaaatatat
     5281 attcgagtgc tatgccaaag gacatggcta tcgtaaaatt gccaatgcat tgaatcacaa
     5341 aggatatgtc actaaaaagg ggaaaccttt cagtattagt tcaatcacat acatcttagc
     5401 taaccctttc tatatcggca aaattcaatt tgcgaaatac aaagattgga gtgaaaaacg
     5461 tcgtaaaggg ctgaatgata aaccagtgat agctgaaggt aagcattccc ccattattaa
     5521 tcaagattta tgggataaag tacaaatgcg taagaaacaa gtcagtcaaa aaccccaagt
     5581 ccatggcaaa ggaacgaatc tgcttacagg cattattcac tgtccccaat gtggcgcacc
     5641 tatggcagca agcaatacca cgaatactct taaagacggg actaagaaac gtattcgtta
     5701 ctattcatgt agtaattttc ggaacaaggg ttccaaagta tgttcggcaa acagtgtaag
     5761 agctgatgtg attgaagatt atgtgatgaa gcaaatactt gaaatagtca aaagtgataa
     5821 agtcattcaa cgcgtagtaa cacacgtcaa tcaagaaaat caagttgatg gcgctgcact
     5881 tcatcacgat attgcttata agcaacaaca atatgatgaa gtacaaatca aaataaataa
     5941 cttgattaaa accatcgagg ataatccgga cttaacatca gtaatcagac caagtattca
     6001 aaaatatgaa aagcaactca atgacattac gaatcaaatc aaccaactca aaaatcaaca
     6061 aaatgaagat aagactttat ttgatgccaa agaaatcagt aaactattac aacacatctt
     6121 tcatgatatt aagcacatag aaaaatctcg actcaaagca ttgtatctat cagtgattga
     6181 tcgcattgat attaaaaaag atggtaatca taaaaaacaa ttctatgtca cactcaaact
     6241 taataacgaa atcattaaac aacttttcaa taataaacaa ctcgacgaag tgcatctcag
     6301 cacttcgtct ttatttttgc cccaaacact atatcttact atttagctaa tagaatcatt
     6361 gttataacaa taattttcac tatattcttt cgcttatttt atctaactat tttcaagaat
     6421 tgctctttat caatttcaat aacacttatc taatgaaaca gttattgtcg taattttttc
     6481 ataactatac aatgcttata ttgaaattgc taaagaacat cccacaatga ttttttatcg
     6541 ggaaatgatt gggatttgca gattcgaaga aacagaagga ttgtgcaagc acaatcccaa
     6601 aatcccgtta ctttttataa ataaataata tatataagga agcagagaca caaccctatc
     6661 atcaatactt ataaataatg agttgattgt ttgtcaaaat aggcggaatt tcaggatttc
     6721 cttggcatac caacgtttgt agcgggtttt tatatggaat tttctcttat tcccaccggg
     6781 attcgtaata aatgacaaat caaatatata ttatctcaat gatgtatatg aactacatca
     6841 ctattacact atctaggagg caataatatg aaaaccatca ctcaagaact caaacaatat
     6901 ataacccacc tattccaact atctaataac gaagcttggg aatgtgaagc gctggaggaa
     6961 gcagcagaaa atattctacc ggaacgtttt attaatgatt ctccactggt acatctcaca
     7021 ctcgagactt atacctacta taatgatgaa ctacatgagc taagtatcta tccatttcta
     7081 atgtatgcca ataaccaact catcagtgtc ggatacttag accattttga catggacttt
     7141 ctgtacctta cagatactca aaatatcatt attgatgaac gtcatttact aaaacaagga
     7201 ggtcaggacc atgaataatt ggatcatagt tgctcaatta tccgtcacag ttatcaacga
     7261 aatcatcgac atcatgaaag aaaagcagaa gggagggaaa tagcatgaaa atcaatcgat
     7321 acatcacaag aggaattaat gaaagtattc cactagacct tcaaatctta ctttggcaca
     7381 tggtagaaaa aaaagataac cagcctcata cagattacct acacattttt aaattacaag
     7441 aagatgagaa tatactctca atcacacatg aacaagaaca acccacatac aaattggaat
     7501 atcactatac aaactatgaa aaaaatcaaa atgcattacc taagaaagtc tacgtcattc
     7561 gtgaagatga cgtggacgct ttttattatg tgatgctttt accagaagaa tactaaagga
     7621 agtgacgatg atgaatacaa tcaaaagtac gatacacaca gaagccatat ttagcgatga
     7681 tgaacagcac cgctatttac tcaagaaaac atgggatgaa aagaaagctg catgcacagt
     7741 gataacgatg tatcctcatt tagacggtgt attatcactc gatctcacaa ctgttcttat
     7801 cctcaaccaa ttagccaatt cagaacgata cggtgctgta tatcttgtaa atctattctc
     7861 taatattaga acacccgaaa acctcaaaca tatcaaaaat ccatacgatg agcacactga
     7921 tattcatttg atgaaagcga ttagtgaaag tgacacagtg attcttgctt atggtgccta
     7981 tgcgaagcga ccagttgtta tcgaccgtgt cgaacaagtg atggaaatgt taaaacctca
     8041 taaaaagaaa gtaaaaaagc tcatcaatcc agtaacaaat gaaattatgc atccactcaa
     8101 ccctaaggca cgtcaaaaat ggattttgaa atcatagcaa atacatggag gtcaatatgg
     8161 aaacaaaaca aatcaacatc gttttactgt aaatgatatt gttgaaacaa ttcctgacaa
     8221 tgaattcgac gaattcagac atcaccgtgg tgcaacttcg taccatccta aaatgatgtt
     8281 aaaagtgatt ctatatgcct acacacaatc tgtattctca ggtcgtaaaa tagaaaaaat
     8341 gcttaatgat agcatccgaa tgatgtggct atcacaaaat caaaaacctt cttataaaac
     8401 aattaatcaa tttagagtaa atccaaaagt agatgcttta ttagaatctt tatttattca
     8461 attttacagt cagtgtgtaa aacaaaatct tatagatgat aaagctattt ttattgatgg
     8521 tacaaaaatt gaagcaaatg ccaatcgata tacatttgta tggaaaaaga gtattcaaaa
     8581 ccatgaatca aagatgaatg aggattctaa agccctctac catgaattgg taaccaataa
     8641 aatcataccg gaaattaaag aagatcatga taatgaatta acaaaagaag aaatagattt
     8701 gattggtagt cacttagata aagaaatcga agatttaaac caacatatca acaatgaaaa
     8761 atgtactaaa acaagaaaac aaatacgtct caaaagaact aaaatcaaaa aatacaaaaa
     8821 gcaaatcaat gattattttg agcgaaagta tcgatacgaa tttcaaaaat ctattttaaa
     8881 ggatagaaat agttattcta agacagatta tgatgcgaca tttatgagaa tgaaagaaga
     8941 tcacatgaaa aatggacaac ttaagccagg gtataattta caaatagcaa caaattccca
     9001 atttgtttta tcttataatg tgtatcaaaa tccaacggat actagaacga tgattccatt
     9061 tttaaattca attcaagaga cctacggtca tttacctgaa tatattgtag ctgatgcagg
     9121 ttatggtagt gaatcaaatt ataaggcaat tatagatgac tttaatcgaa cgccactcat
     9181 aacatatgga atgtttataa aagataaaac taaaaaatat aaaagtgaca tctttaatac
     9241 tcaaaattgg aactatgacg aaattaatga cgaattcatt tgtccgaata ataaacggct
     9301 aggttttaaa agatatgcct atcgtcatga taagtatggt tataagcgag acttcaaatt
     9361 atatgaatgt gatgattgtt cagaatgtcc tctgaaaaat caatgtatga acttcaattc
     9421 aaaaacaaac aaaaaaataa tgaagaatta taactgggaa tattttaaat cccaaattaa
     9481 caaaaagctt tcagaaccag aaacaaaaaa tatctacagt caaagaaaaa ttgatgtgga
     9541 acctgttttt ggatttatga aggctatttt gggtttcact cggatgtctg tccgaggact
     9601 caataaagtc aaaagagaac ttggttttgt attaatggca cttaatataa gaaaagtagt
     9661 agctcaacga gctgaaaata atcaaaaaat ttataaaaaa gacaatttct atattatttc
     9721 aatagaaatt gtcttttttc acttatccaa gaactttatg tcccggactc attatgattt
     9781 atttttttat tgctgacttg ccattaattc tgcttgctct acaacttgtt ccacagccat
     9841 tttttgaaga tctggtggat agccatattt ctttaatagg cgtctaactt gaactctcat
     9901 tttagcttta gcactgtctc gtttagacca atcaacactc atgttatcct taactgtttt
     9961 agttaactca tgtgcaatgg ctcttaactc tttatcgccc aaagcttctt tagctgtttc
    10021 atgtgaagct cgatatacta catacaatat acgcaacaat acgcttgttt cgattaggtt
    10081 taggcattga atcataaagt gcaatatact taacacgttc tttaatattt gaattaaaac
    10141 ctagtaaata ttgtgctgcc acattattta tgtgctgaga ttttaaaata gagcatttta
    10201 atatcgattc accataacgt atatgttcat ggcgattcaa aatttttaaa acgtttctat
    10261 cacatacttt ttcacagtca ttgtccatca ttgttttact tatatatagt gcaggattaa
    10321 accagaatat cattttaaaa acaacataaa gctggttgaa tattaagtca tgacttttca
    10381 catgtgatag ttcatgtaga ataatatatt caatttcttt gtcattcatg gtttcgacta
    10441 cgacagttgg tagtacaatt tgggatttca ctaaaccaaa taccatcgga ttatcaatgt
    10501 ttgaactata actaattgtt atatgctttt tgtagaactg catcttactt tgacatactt
    10561 taagtcgttc attaagatat gacgattcca atgacgaact tttaataaca tcaatttgtc
    10621 ggaatgcctt aatcatataa aataagcaca acaaactacc aaatacccat atcaaaagaa
    10681 tcatatacgt tatatttgag gtctcaaact gattaacatt aattgctaag tctttcgtaa
    10741 cagatgattg ttgaccatct aacatatgac taaccgaaga agtcgtgtca gatacatttc
    10801 gattcatcat atcttttgaa aatgtaaaat tcgatatttt gtaaaatggt attaatggaa
    10861 ttaacgtgga gacgagcact aataaccaaa tcttatgtga cataatattt tgagtatatt
    10921 ttatatagag cattctcact aaaaaaatta cacatatcgt gagcaatgaa ctgattatac
    10981 ttaacattaa aaaagatgat aacaccttct acacctccat atcacaaaaa ttataacatt
    11041 attttgacat aaatactaca tttgtaatat actacaaatg tagtcttata taaggagtat
    11101 attgatgaaa aagataaaaa ttgttccact tattttaata gttgtagttg tcgggtttgg
    11161 tatatatttt tatgcttcaa aagataaaga aattaataat actattgatg caattgaaga
    11221 taaaaatttc aaacaagttt ataaagatag cagttatatt tctaaaagcg ataatggtga
    11281 agtagaaatg actgaacgtc cgataaaaat atataatagt ttaggcgtta aagatataaa
    11341 cattcaggat cgtaaaataa aaaaagtatc taaaaataaa aaacgagtag atgctcaata
    11401 taaaattaaa acaaactacg gtaacattga tcgcaacgtt caatttaatt ttgttaaaga
    11461 agatggtatg tggaagttag attggaatca tagcgtcatt attccaggaa tgcagaaaga
    11521 ccaaagcata catattgaaa atttaaaatc agaacgtggt aaaattttag accgaaacaa
    11581 tgtggaattg gccaatacag gaacagcata tgagataggc atcgttccaa agaatgtatc
    11641 taaaaaagat tataaagcaa tcgctaaaga actaagtatt tctgaagact atatcaaaca
    11701 acaaatggat caaaattggg tacaagatga taccttcgtt ccacttaaaa ccgttaaaaa
    11761 aatggatgaa tatttaagtg atttcgcaaa aaaatttcat cttacaacta atgaaacaga
    11821 aagtcgtaac tatcctctag gaaaagcgac ttcacatcta ttaggttatg ttggtcccat
    11881 taactctgaa gaattaaaac aaaaagaata taaaggctat aaagatgatg cagttattgg
    11941 taaaaaggga ctcgaaaaac tttacgataa aaagctccaa catgaagatg gctatcgtgt
    12001 cacaatcgtt gacgataata gcaatacaat cgcacataca ttaatagaga aaaagaaaaa
    12061 agatggcaaa gatattcaac taactattga tgctaaagtt caaaagagta tttataacaa
    12121 catgaaaaat gattatggct caggtactgc tatccaccct caaacaggtg aattattagc
    12181 acttgtaagc acaccttcat atgacgtcta tccatttatg tatggcatga gtaacgaaga
    12241 atataataaa ttaaccgaag ataaaaaaga acctctgctc aacaagttcc agattacaac
    12301 ttcaccaggt tcaactcaaa aaatattaac agcaatgatt gggttaaata acaaaacatt
    12361 agacgataaa acaagttata aaatcgatgg taaaggttgg caaaaagata aatcttgggg
    12421 tggttacaac gttacaagat atgaagtggt aaatggtaat atcgacttaa aacaagcaat
    12481 agaatcatca gataacattt tctttgctag agtagcactc gaattaggca gtaagaaatt
    12541 tgaaaaaggc atgaaaaaac taggtgttgg tgaagatata ccaagtgatt atccatttta
    12601 taatgctcaa atttcaaaca aaaatttaga taatgaaata ttattagctg attcaggtta
    12661 cggacaaggt gaaatactga ttaacccagt acagatcctt tcaatctata gcgcattaga
    12721 aaataatggc aatattaacg cacctcactt attaaaagac acgaaaaaca aagtttggaa
    12781 gaaaaatatt atttccaaag aaaatatcaa tctattaact gatggtatgc aacaagtcgt
    12841 aaataaaaca cataaagaag atatttatag atcttatgca aacttaattg gcaaatccgg
    12901 tactgcagaa ctcaaaatga aacaaggaga aactggcaga caaattgggt ggtttatatc
    12961 atatgataaa gataatccaa acatgatgat ggctattaat gttaaagatg tacaagataa
    13021 aggaatggct agctacaatg ccaaaatctc aggtaaagtg tatgatgagc tatatgagaa
    13081 cggtaataaa aaatacgata tagatgaata acaaaacagt gaagcaatcc gtaacgatgg
    13141 ttgcttcact gttttattat gaattattaa taagtgctgt tacttctccc ttaaatacaa
    13201 tttcttcatt ttcattgtat gttgaaagtg acactgtaac gagtccattt tcttttttta
    13261 tggatttctt atttgtaatt tcagcgataa cgtacaatgt attacctggg tatacaggtt
    13321 taataaattt aacgttattc atttgtgttc ctgctacaac ttcttctccg tatttacctt
    13381 cttctaccca taatttaaat gatattgaaa gtgtatgcat gccagatgca atgatacctt
    13441 taaatctact ttgttctgct ttttctttat ctatatgcat atattgagga tcaaaagttg
    13501 ttgcaaattg gataatttct tcttctgtaa tatgaaggct ttttgttttg aatgtttctc
    13561 ctactataaa atcatcgtat ttcatatatg tctctctttc ttattcaaat taatttttta
    13621 gtatgtaaca tgttaaaggt aagtctaccg tcactgaaac gtaagactca cctctaactt
    13681 tctattgaga caaatgcacc attttatctg cattgtctgt aaagatacca tcaactcccc
    13741 aattagcaag ttggtttgca cgtgctggtt tgtttacagt ccatacgttc aattcataac
    13801 ccgcttcttt taccattttt acttttgctt tagtaagttt ggcatcttca gtgtttacta
    13861 ttttagcatt acagtaatct aaaagtgttc tccagtcttc acgaaacgaa gttgtatgga
    13921 atataactgc tctgttatat tgtggcatga tttcttctgc aagtttaaca agcacaacat
    13981 taaagcttga aatgagcact tcttgattct gatttaagtt tgttaattgt tcttccactt
    14041 gcttaaccat acttttagaa agtgctagtc cattcggtcc agtaatacct tttaattcta
    14101 catttaaatt catattatat tcatttgcta tttttactac atcatcgaaa gttggcaaat
    14161 gttcatcttt gaatttttca ccaaaccaag atcctgcaga agcatcttta atttcatcat
    14221 aattcaattc agttatttcc ccggacatat ttgtagtccg ttctaaataa tcatcatgaa
    14281 tgataatcag ttgttcatct tttgtaattg caacatctaa ctccaaccag tttatacctt
    14341 ctacttctga agcagcttta aatgatgcaa ttgtattttc cggagcttta ctaggtaatc
    14401 ctctatgtcc atatacagtt agcatattac ctctccttgc atttttattt ttttaattaa
    14461 cgtaactgta ttatcacatt aatcgcactt ttatttccat taaaaagaga tgaatatcat
    14521 aaataaagaa gtcgatagat tcgtattgat tatggagtta atctacgtct catctcattt
    14581 ttaaaaaatc atttatgtcc caagctccat tttgtaatca agtctagttt ttctgtaccc
    14641 cttatctgca attttactta ggattgcttt taacttaccc cttatcagca attttactga
    14701 gaactgcttt taacgcacct cttatctgca attttgccta gaactgcttt taacgtacct
    14761 cttatctgca attttactga gaactgcttt taacttaccc cttatcagca attttgcatg
    14821 gaattgcttt taacgtacct cttatctgca attttactta gaactgcttt taacaaacct
    14881 cttatctgca attttactta gaactgcttt taacgtacct cttatctgta attttactga
    14941 gaactgcttt taacaaacct cttatctgca attttactta gaattgcttt tactattcct
    15001 cttattagta taatctcagt aagaatgcgt ataaaaatga aaattacaac cgattttgta
    15061 agtgctgacg cctgagggaa tagtatgtgc gagagactaa tggctcgagc cataccccta
    15121 ggcaagcatg cacgtacaaa atcgtaagat aaaaaaataa gcatatcact gtaaacttta
    15181 aaaaatcagt ttagtgatat gcttatttat ttcgagttag gatttatgtc ccaagctcat
    15241 caagcacaat cggccactag tttatttctc tatcttatat gttctgatat ggtcttctat
    15301 actgtataag tatacttttg aatatggatc ttgtgtcaat tcacgttcga aatcaaattc
    15361 ttgattatca aatctgttaa agaatgtttc gtattcttcg actgataatt gctctctaga
    15421 ttctagcata tttaagtgtt tctctttatc taatgctttg tcatatcctt taacgattga
    15481 accactaaag atttctccta ctgctcctga accataacta aatagacata ctttctcttc
    15541 tggttggaat gtgtggttct gtaataacga aattaaactt aagtataatg atcctgtata
    15601 aatgttacca acatctctat tccataatac ggttctgttg caaagttgaa tttatagtat
    15661 aattttaaca aaaaggagtc ttctgtatga actatttcag atataaacaa tttaacaagg
    15721 atgttatcac tgtagccgtt ggctactatc taagatatac attgagttat cgtgatatat
    15781 ctgaaatatt aagggaacgt ggtgtaaacg ttcatcattc aacggtctac cgttgggttc
    15841 aagaatatgc cccaatttt
//
LOCUS       DAMQYT010000041        15774 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMQYT010000041 DAMQYT010000000
VERSION     DAMQYT010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15774
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.041"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(167..1069)
                     /gene="cvfB"
                     /locus_tag="PGA33_001022"
     CDS             complement(167..1069)
                     /gene="cvfB"
                     /locus_tag="PGA33_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HDG6472847.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDYLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKIALTKKGWSRMDSKE"
     gene            1216..2817
                     /locus_tag="PGA33_001023"
     CDS             1216..2817
                     /locus_tag="PGA33_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDG6472848.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNISFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSYKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            3677..4882
                     /locus_tag="PGA33_001024"
     CDS             3677..4882
                     /locus_tag="PGA33_001024"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDG6472849.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGMNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIISNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            4946..5935
                     /locus_tag="PGA33_001025"
     CDS             4946..5935
                     /locus_tag="PGA33_001025"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDG6472850.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            5937..6824
                     /locus_tag="PGA33_001026"
     CDS             5937..6824
                     /locus_tag="PGA33_001026"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDG6472851.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNIEALKTHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLRETYDTFKAGENE"
     gene            6821..7543
                     /locus_tag="PGA33_001027"
     CDS             6821..7543
                     /locus_tag="PGA33_001027"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDG6472852.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKGADVAIDFSNPNLLFPLLDEDFHLPLVVATTGEKEKLLNKLDELSQNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            7570..8289
                     /gene="dapD"
                     /locus_tag="PGA33_001028"
     CDS             7570..8289
                     /gene="dapD"
                     /locus_tag="PGA33_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDG6472853.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDAKKEIVAALRKLND"
     gene            8432..9583
                     /locus_tag="PGA33_001029"
     CDS             8432..9583
                     /locus_tag="PGA33_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDG6472854.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            9588..10673
                     /locus_tag="PGA33_001030"
     CDS             9588..10673
                     /locus_tag="PGA33_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDG6472855.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTLKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            10663..11928
                     /gene="lysA"
                     /locus_tag="PGA33_001031"
     CDS             10663..11928
                     /gene="lysA"
                     /locus_tag="PGA33_001031"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDG6472856.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
     gene            complement(12168..12569)
                     /gene="msa"
                     /locus_tag="PGA33_001032"
     CDS             complement(12168..12569)
                     /gene="msa"
                     /locus_tag="PGA33_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarA expression modulator protein Msa"
                     /protein_id="HDG6472857.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNINILAAMMIVLVIPIMISGI
                     LFFKTNIDKTYIFFNIIFIDFYYYIYNVHLMTLPKFNNYIKAEMMELEDIDVLITSKD
                     FGFDEILFYTLYLLLILIVLYYLKKQVKHKI"
     gene            complement(12766..12966)
                     /gene="cspA"
                     /locus_tag="PGA33_001033"
     CDS             complement(12766..12966)
                     /gene="cspA"
                     /locus_tag="PGA33_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HDG6472858.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
     gene            complement(13137..13445)
                     /locus_tag="PGA33_001034"
     CDS             complement(13137..13445)
                     /locus_tag="PGA33_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDG6472859.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFIVLQNNEVYYKLPIID
                     "
     gene            13608..13877
                     /locus_tag="PGA33_001035"
     CDS             13608..13877
                     /locus_tag="PGA33_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDG6472860.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            13896..14525
                     /locus_tag="PGA33_001036"
     CDS             13896..14525
                     /locus_tag="PGA33_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDG6472861.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            14557..15693
                     /locus_tag="PGA33_001037"
     CDS             14557..15693
                     /locus_tag="PGA33_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDG6472862.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
BASE COUNT         5624 a         2482 c         2755 g         4913 t
ORIGIN      
        1 taatagaaat cagctttttt acattgccta agaacttaat gtcccagcct attcgtttga
       61 attatttaat tttttacttt gaaaggaatg tttaaaaaac tacttcatct gtccgattta
      121 atattgaata aaaaaaacac gcatcctacg acacgtgtaa aaatgattat tcttttgagt
      181 ccattcgact ccaacctttt ttagttaaag cgattttacc tgtttctata ttaataatct
      241 tctgtttata taagtgaccg attgcacgtt tgaatgaacc tttactcata ttgaatactt
      301 ctttaatcgc ttcagggctt gatttgtccc agaatggtaa ttcaccatca tattcaacta
      361 gtaaatcaaa gatgacttgg ccgtcatcgt ctaaacgttc atgtgcaagt ggtaaaaatg
      421 aaccatttaa ctcaccttta tcattatgcc cgataattct aacttgaaca gattcaccta
      481 atcttggttc agctttacgt tctgattcat gtacgaaaat tttgtaacct gattcgctta
      541 ataaaaagct accaattcgt aatacgcggt aaggtttggc ttcaatgact tcgtttttta
      601 aattatcgtc gtgtacaggt gtaaacatat tttctacaac agattcactc gctaaacgtc
      661 catacatatg attctcacgg tcaattcgta atgtaactag caaataatca ccaggttgtg
      721 gccatagtga tttcactttt ggtaaatctt cccatggtac taacacttca cggggtaatc
      781 caacatctat acgtgcccca tcgcgatccg ttttaagtac tttagcaaag tcatatttat
      841 ctttcgtaat atcaggcata ttttgagttg caaataattc acctgaacgg tttggataaa
      901 tgaagaaact atattcttca cctacttcta attcatcatc atcgttcatt tctgattggt
      961 ttaactttac gttttcaccg tttggtcctt ttaaaaggta agttgaacct tgtaacccta
     1021 ctacttcaag gaattctata gaacctacta tatctttgtc taatgccatg ttattctcct
     1081 tcgattcttg tattctttat tattataaca tgtgtgagtc attctatcat tacaacttat
     1141 atgaggtcga aactgtgacg ataatatgat ataattcttc cgttagaata tcgaatgaaa
     1201 aaaaggagaa aatgcatgtt acaagtaact gatgtgagtt tacgttttgg agatcgtaaa
     1261 ctatttgaag atgtaaatat taaatttaca gaaggtaatt gttatggatt aattggtgcg
     1321 aatggtgcag gtaaatcaac attcttaaaa atattatctg gtgaattaga ttctcaaaca
     1381 ggacatgttt cattagggaa aaatgaacgt ctagctgttt taaaacagga ccactatgct
     1441 tatgaagatg aacgcgtgct tgatgttgta attaaaggtc acgaacgtct ttatgaggtt
     1501 atgaaagaaa aagatgaaat ctatatgaag ccagatttca gtgatgaaga tggtatccgt
     1561 gctgctgaac ttgaaggtga atttgcagaa atgaatggtt ggaatgctga agctgatgct
     1621 gctaaccttt tatctggttt aggtatcgat ccaactttac acgataaaaa aatggctgaa
     1681 ttagaaaaca accaaaaaat taaagtatta ttagcgcaaa gtttattcgg tgaaccagac
     1741 gtactattac tggatgagcc tactaacggt ctcgatattc cagcaatcag ttggttagaa
     1801 gatttcttaa ttaactttga taatactgtt atcgtagtat cgcatgaccg tcatttctta
     1861 aataatgtat gtactcatat cgctgattta gacttcggta aaattaaagt ttatgttggt
     1921 aactatgatt tttggtatca atctagtcag ttagctcaaa agatggctca agaacaaaac
     1981 aagaaaaaag aagaaaaaat gaaagagtta caggacttta ttgctcgttt ctcagctaac
     2041 gcttctaaat ctaaacaagc aacaagtcgt aaaaaacaac tcgagaaaat tgaattagat
     2101 gatattcaac catcatcaag aagatatcct ttcgttaaat tcacacctga gcgcgaaatc
     2161 ggtaatgact tactaatcgt tcaaaatcta tctaaaacga ttgacggtga aaaagtatta
     2221 gataatattt cattcacaat gaatccaaat gataaagcaa ttttaattgg ggatagtgaa
     2281 attgcgaaaa ccacattgct taaaatatta gccggtgaaa tggaaccaga cgaaggttca
     2341 tataaatggg gtgtaacaac gtcattaagt tacttcccta aagataactc agaattcttt
     2401 gagggcgtta atatgaacct tgtagattgg ttaagacaat acgcaccaga agatgagcaa
     2461 actgaaacat ttttacgcgg cttcttaggc cgtatgctat ttagtggaga agaagttaag
     2521 aaaaaagcta gtgtactttc aggtggagaa aaagtacgtt gtatgctaag taaaatgatg
     2581 ttatcaagtg caaacgttct tttacttgat gaaccaacaa accacttaga cttagaaagt
     2641 attactgctg ttaatgatgg acttaaatca ttcaaaggtt ctattatctt tacttcatat
     2701 gactttgaat ttatcaatac aatcgcaaac cgtgttatcg atttaaataa acaaggtggc
     2761 gtttcaaaag aaattccata tgaagaatat ttacaagaaa tcggcgtttt aaaataaaca
     2821 taatatgtag aatttctttt cgaaaatctc tgtattgagg cccgaccaac ttgcacatta
     2881 ttgtaagttg gcttactgtc accttctata ttgggactta ttccgcaact tgtattgttt
     2941 gtagaaatta ggaatccaat ttctctatgt gttgcaccga caaggattga aaaaagctag
     3001 ctacaaagtc attttcattc agtcaactac tgtcaatata acattgcagg gcctagggca
     3061 ttgattcatg tccgagacta tattcaattt aatagacatt aactgggcgt ctcgatgcta
     3121 atatttatag catcgaggtg tcctttttat tttatttttt aacacatcag catagtgaac
     3181 accccttctc taaattacaa atacagttat caacgcatta aaagagtaca tttatttaat
     3241 gtgcatattg attatgctat aagtattttg aattttattt ttcaaaaagt ctcattatca
     3301 ccacctttta aaatataagc aacgctataa cttgaatttt caggttattc caaaaaatct
     3361 aaattatcgg tttacattca tatttgtcat atgtataatg aacacatacc aatttaataa
     3421 tattttgatg aggcgcatca atcatgagta aagtttagat tactgtctgc taacagctaa
     3481 atttgaaagg gtgcgatgcc gaagcaatta taatagcagt tataatttgt tggacttttt
     3541 ggttaagagc tgagagtttg tcattattta aaaataatgg agtgcatcac ttgtatatag
     3601 attaagagca agttcgcatt ccgaacttgt tcttttttta ttattgtgtg cccttcctaa
     3661 caattaggag gattatatgg taacaagaag tgtgttgaaa tttggcggat catccgtcag
     3721 tgattttaca aaaataaaaa ggatcgctga aatgttaaag gagcgagtca atcaagatga
     3781 acaattaatt gtcgttgtaa gtgctatggg taacacaaca gatcaattaa tgacgaatgt
     3841 atcaaccttg actaaagcac caaaacaaca agaactggca ttattattga caaccggaga
     3901 gcaacaaact gtatcttatt tatcaatggt attaaatgat atcggtatga atgccaaagc
     3961 aatgactggc tatcaagcgg gtattaaaac cattggccat catttaaaaa gtaaaattgc
     4021 tcaaattaat cctcaaacat ttgaacaagc ctttcaagaa aacgatattt tagtagttgc
     4081 tggatttcaa ggcatcaatg aacatcagga attaacaact ttaggcagag gtggttctga
     4141 tacgaccgct gtggcacttg ctgttagtaa tcaaatacct tgtgaaattt ataccgacgt
     4201 tgatggtgtg tatgccactg acccaagact tttaccaaag gctaaacgac tagacatcgt
     4261 ctcatatgaa gaaatgatgg aaatgagcgc tttaggtgct ggtgtacttg aaacaagaag
     4321 tgttgaatta gctaaaaact ataatatccc tttatattta ggaaaaactt tatcgaacgt
     4381 gaaaggaaca tggattatat caaatgaaga aatattagag aaaaaagcag ttactggtgt
     4441 ggctttggat aaacatatga tgcatgtaac aattagttat cccctacctg acaatcagct
     4501 acttacccaa ctatttacgg aacttgaaga aggtgctgta aatgttgata tgatttcaca
     4561 aatcgtcaac ttggatgggc tacaactatc cttcacgatt aaagatagtg attttcatca
     4621 aatttctatg attcttgaaa cattaaagaa tcaatatgaa gcattagctt ataaaatcaa
     4681 tgagcattat gtcaaaattt cattaattgg ctcaggcatg cgtgatatgt caggtgtggc
     4741 atcaaaagca tttttgacat taattgaaaa taatatacct ttctaccaaa caacaacatc
     4801 tgaaataagt atttcatacg tcattgatga ttttaatggg caacaagcgg tagaaaaact
     4861 atatgacgca tttaacattt aatggtaaaa tgattgttaa aatattctaa aaattggaaa
     4921 ttattataaa atggagtgac aagttatgac aaagttagca gttgtgggtg caacaggatt
     4981 agtaggtaca aaaatgttgg agacattaaa tcgtaaaaat attcctttcg atgaattagt
     5041 attattttca tcagcacgtt ctgcagggca agaagttgaa tttcaaggaa aaacatatac
     5101 agttcaagaa ttaactgatg ctcgtgcaag tgaacatttc gattatgtat taatgagtgc
     5161 tggtggcggt acaagcgaac actttgcccc actttttgaa aaagctggtg caatcgttat
     5221 agacaattca agtcaatggc gtatggcaga agatattgat ttaatcgttc cggaagtcaa
     5281 tgaacctaca tttacaagag gtatcattgc caatccaaac tgctctacga ttcaatctgt
     5341 tgtacctcta aaagtattgc aagatgctta tggtttaaaa cgagtggcat atacaacata
     5401 tcaagctgta tcaggttcag ggatgaaagg taagaaagat ttagctgaag gtgtaaatgg
     5461 taaagcacca gaagcatatc cacatccaat ttataataat gtgttaccgc atattgatgt
     5521 gtttttagaa aacggatata caaaagaaga acaaaaaatg attgatgaga cgagaaaaat
     5581 tttaaatgcg ccagacttaa aagtaacagc aacatgcgca cgtgtgcctg ttcaagatag
     5641 tcatagtgtt gaaattgatg taacgcttga caaagaaaca acagcagaag atattaaagc
     5701 gttatttgat caagatgacc gcgttgtttt agtagacaat ccagagaaca atgaatatcc
     5761 aatggcaatc aattctacta ataaagatga agtgtttgtt ggccgtatac gtagagatga
     5821 ttcattagaa aatactttcc atgtatggtg tacatcagac aatttattaa aaggtgctgc
     5881 attaaatgct gtacaagtat tggaacaagt tatgcgttta aaaggagcga attaaaatga
     5941 cacatttatt tgagggtgtt ggcgttgcac ttacaacccc ttttacaaat aacaaagtta
     6001 atattgaagc tttgaaaaca cacgttaatt ttttactaga aaataatgcc caagcaatca
     6061 tcgttaatgg aactactgct gagagcccta ctttaacaac agatgaaaaa gaacgcattc
     6121 taaaaacagt tattgatctt gtagataaac gtgttcctgt catagcagga actggcacta
     6181 atgatactga aaagtcaatc caagcttcaa tccaagctaa agccttaggg gctgatgcaa
     6241 ttatgttaat tacgccctac tacaacaaaa cgaaccaacg tggtttagtc aaacactttg
     6301 aagcgattgc agatgctgtg aaattaccag tcgtgctgta caatgttcct tcaagaacga
     6361 acatgacaat tgaaccagaa actgtagaaa tattaagtca acatccttat atagttgctt
     6421 taaaagatgc tacgaatgat tttgagtatt tagaagaagt gaaaaagcgc attgatacaa
     6481 attcatttgc attatatagt ggcaatgatg acaacgtcgt cgaatactat caacgtggcg
     6541 gtcaaggggt tatctctgtt attgccaatg tcattcctaa agaatttcaa gcgttatacg
     6601 atgctcaaca aagtggatta gatattcaag atcaatttaa accaatcggc acactgttat
     6661 cagctttatc agttgatatt aacccaattc ctattaaagc tctaacaagt tatttaggat
     6721 ttggaaatta tgaattacgt ctaccattgg ttagcctaga agatacagat actaaagtgc
     6781 ttcgtgaaac atatgacaca tttaaagcgg gtgaaaatga gtgaaaatat tactaattgg
     6841 ctatggcgca atgaatcaac gcgttgctag attagcagaa gaaaaaggac atgaaatcgt
     6901 tggggtcatt gaaaatacac cgaaagcaac aacgccatat caacaatatc aacatattgc
     6961 agatgttaaa ggtgccgatg ttgcaataga tttttcaaat ccaaatctgc ttttcccttt
     7021 attagatgaa gattttcatt tgccattagt tgtggcaaca actggcgaga aagaaaaact
     7081 acttaataag ttagatgaat tgagtcaaaa tatgcctgtg tttttcagcg cgaacatgag
     7141 ttatggcgtt catgcattga ctaaaatttt agcagctgct gttcccctac ttgatgattt
     7201 cgacatcgaa ttgactgagg cacatcataa taaaaaagta gatgcaccaa gtggtacgtt
     7261 agaaaaattg tatgatgtga tcgtatcttt gaaagaaaat gtaacacctg tgtatgatag
     7321 acatgaatta aatgaaaaac gccagccaca agatattggt atacattcta ttcgtggagg
     7381 tacgattgtc ggtgaacatg aagttctatt tgctggcact gatgaaacga ttcaaatcac
     7441 gcatcgtgca caatcaaaag atatttttgc gaatggtgca atacaagcag cagaacgctt
     7501 agttaataaa ccaaacggct tttatacgtt tgacaactta taaacatatt aaaggagatc
     7561 gattatttta tggtacaaca tttaacagct gaagaaatta ttcaatatat aagtgatgct
     7621 aaaaagtcta caccaataaa agtatattta aatggtaatt ttgaaggcat cacatatcca
     7681 gaaagtttta aagtatttgg ttcagaacaa tctaaagtaa tcttttgtga agcggatgat
     7741 tggaaacctt tttacgaagc atatggtagt caattcgaag atatagaaat tgaaatggat
     7801 cgtcgcaatt ctgctattcc attaaaagac ttaacaaata cgaatgcacg aattgaacca
     7861 ggtgcgttta ttagagaaca agccattatt gaagatggtg ctgtcgttat gatgggcgca
     7921 acaattaata ttggcgcagt cgttggcgaa ggtacaatga ttgatatgaa tgctactctc
     7981 ggtggtcgtg ctacaactgg taaaaatgta catgtagggg ctggcgcagt attagcaggt
     8041 gtgattgaac cccctagtgc ttcaccggtt ataatcgagg atgatgtatt aatcggtgca
     8101 aatgcagtta ttttagaagg tgtacgtgtt ggtaaaggtg ctattgttgc agctggcgcg
     8161 attgtgacac aagatgtacc agctggtgca gttgttgctg gtacacctgc aaaagtgatt
     8221 aagcaagctt ctgaagtaca agatgctaaa aaagagattg tagcagcatt aagaaaactg
     8281 aatgactagt gacgtcaacg tataataatt tcaaggttga gatacgatta tgtctcaacc
     8341 ttattgttta gcattgaata aacttatgat cacaaatttc gaaatacgtt acactattta
     8401 tatagtaatt taacacaaag gattgataat tatgaatgaa ttagaatttg ttacgaaaca
     8461 tcgccgtcat ttacatcaac atcctgaatt aagcttacat gaatttgaaa caactgctta
     8521 tattaaagcg tttttagata gtttaaatat taaatacgat tgcccattgg aaactggcgt
     8581 cattgcatac ttagaaggta atggctcaca tacgatagcg tatagagctg atattgatgc
     8641 gttacctatt ttagaggaaa atgatgtgcc ttatcgcagt caatctgatc atgtgatgca
     8701 tgcttgtgga catgatggtc atacaactgc attaatgctt tttgtacaac gttgcaaaga
     8761 catgcaagat gcaggtcaat taccgcaaaa tgtcgttttc attttccaac ctgcagaaga
     8821 aactggtggc ggtgcaaatc gattaataaa agccggtgcc tttgataagt atccaattga
     8881 agcggtattt ggtattcatg ttaacccatt tgctgatgaa ggcattgcag tgataagaga
     8941 tgaagaaatt acggccagcg caacagagta tcgctttttc ttaacaggcc tgtcaagtca
     9001 tgttgctgat aaagaacaag gtcattcttg tggtgaagca ttacaacatg tattaactca
     9061 aatatcacaa attcaacaat ttcaccttaa cggtttgaaa cgaaatattg ttcatattgg
     9121 tcattttaaa gctggtgaag cgattaacac tgtaccaagt aatggctatt tagaaggtac
     9181 tattcgtaca tatgatattg atgatttaac aatcgttaaa aatcaaatgc acaagatagc
     9241 agaaagtgtc aagcttctgt ttaatgtaga ttgtgaagtt aaatttgcag aaggttatcc
     9301 ccctacaatc aatagtccga aattacgtac tcaaatagag gacgccttaa taaaagctga
     9361 tttaaatgtc tatgacaaac caacgccatt cttatttggg gaagatttta gtttttatgg
     9421 tcaacaacta gctccagctt actttgtttt tataggaaca cgaaatgaag ataaaggttt
     9481 tgtaactggt ttgcacacat cacatttaaa ttttgatgaa aaagtgttaa taaacgtggt
     9541 taatttttac gaaaatttat taaataatta caaagaggtg taatacattg acagcaacat
     9601 ggtctgtaaa taagaaaata tttttacaaa atgcaatcac agtcaaaaac aatcagccat
     9661 taatggcagt tgttaaaaat aatgcatatc actatgacct agaatttgct gtaactcagt
     9721 ttatccatgc aggtatagat acatttagca caacatcact acgagaagca attcaaatta
     9781 gacaacttgc tccagatgca acaatctttt taatgaatgc agtttacgag tttgatttag
     9841 tccgtgaaca tcaaatacat atgactttgc cgtcgttgac atattactat aaccataaaa
     9901 atgatttagc tggtattcat gttcacttag aatttgaaaa tttattacat cggtctggat
     9961 ttaaagattt aaacgaaatt aaagaagtat tgaaagatca ccatcataat caaaatgcaa
    10021 aaatgattat tagtggttta tggacccatt ttggatatgc tgatgaattc gatgtgtcag
    10081 attataatgt tgaacgttca caatggatgg aaattgttga agcactttta tctgaaggtt
    10141 atcagttcga cctaatccat gctcaaaata gtgcgagttt ttatcgggaa ggacaaatat
    10201 tactacccca ccatacacat gcgcgtgtag gtattgcgtt atacggttca agaccatata
    10261 gttcactgaa tcaacatgat atagttcagt cattaacttt aaaagcacat gttattcaag
    10321 tgcgcgaagt acaagctggt gattattgcg gttatagctt tgcctttgaa gtgactaaaa
    10381 acaatacaaa attagctgta gttgatatcg gttatggcga tggaatttta agaactcgtg
    10441 ctaaacatga agcacttatc aatggtaaac gctacccgat acgtgcatta atgatgagcc
    10501 atatgtttgt tgaagtagat ggcaatgtac atgcacaaga tgaagttatt ctttataata
    10561 atgatatccg catcgatgaa tataccttta aaggtgttgg tgcaaattct gaacaattaa
    10621 gtgctatgaa tcatgattct ttaaaaaagg agtacatttc aaatgactgt taaatataat
    10681 caaaatggcg aattaacaat ggatggtatt agtttaaaaa cgattgcaca aagctttggt
    10741 acacctacca ttgtttatga tgaactacaa attagagaac agatgcgccg ttaccatcgc
    10801 gcatttaaag atagtggatt aaaatacaat atttcatacg cctcaaaggc atttacttgc
    10861 attcaaatgg tcaaacttgt agctgaggaa gatttacagt tagatgttgt ttctgaaggt
    10921 gaattatata cagctttaga agcaggtttt gaaccgagtc gcatccattt ccatggtaac
    10981 aataaaacga aacatgaaat taggtatgct ttagaaaata atatcggtta ttttgttata
    11041 gattcattag aagaaattga attaatagac cgctatgcta atgatacggt tcaagttgta
    11101 ttacgagtta atccaggtgt tgaagcacat acacacgaat ttattcaaac tgggcaagaa
    11161 gatagtaagt ttggattatc aattcaatat ggcttagcta aaaaagcaat tgacaaagtc
    11221 caacaatcta aacacttaaa attaaaaggt gtacattgtc atattggttc acagattgaa
    11281 ggtacagaag catttattga aactgctaaa attgttttac gttggcttaa agagcaaggc
    11341 attcaagttg aattattaaa ccttggtggt ggctttggta ttaaatatgt tgaaggtgac
    11401 gaaagtttcc ctatcgaaag tggtattaaa gatattacag acgcaataaa atccgaaatt
    11461 aaagttctag gtatagatgc accagaaata ggtattgaac cgggacgatc aattgtaggt
    11521 gaagctggcg ttactttata tgaagttgga accattaaag aaattccaga gattaataaa
    11581 tatgtttcaa tcgatggcgg tatgagtgat catatcagaa ctgcacttta tgacgcaaag
    11641 tatcaagcat tgcttgttaa tagaaatgaa gaagcagatg acagtgtaac tatagctgga
    11701 aaattatgtg agtctggtga tatcattatt aaagacgcta aattaccttc atcagtcaaa
    11761 cgtggagact atcttgctat attatcaact ggtgcatatc attactctat ggcatccaat
    11821 tacaatcaaa tgcaaaagcc ttctgtgttt ttcttaaaag atggcaaagc acgtgaagtt
    11881 ataaagcgac aatcgttaag acaactcatt attaatgata caaaataaaa ataaacaaag
    11941 taatccccga agcacagaaa attatactgt gactcgggga atttttatgc attatagatg
    12001 actgtagaaa acatgcttgg acatatactc taaaaaagga agaacagcac atttttagtt
    12061 agaaagtatt tatttctttt tgtcattaat cctttatatt tagttagccc tattcaacta
    12121 atttaaagca tcatatacat accacacttt aaacaagcat catgaaatta gattttatgc
    12181 tttacttgct ttttcaaata atataaaacg ataagaatta acaacaaata taatgtataa
    12241 aacagaattt catcaaatcc aaaatcttta cttgtaatta atacatcaat atcttctaat
    12301 tccatcatct ctgctttaat ataattatta aactttggca aagtcattaa atgtacattg
    12361 taaatatagt aataaaagtc gataaatatg atgttaaaaa atatgtatgt tttatcaata
    12421 ttagttttga aaaatagtat cccagaaatc attatcggaa tcactaatac aatcatcatt
    12481 gctgctaaaa tattaatatt taatccaatt gcagataata caccgaaaac taataaatta
    12541 gcaactaaac taaggataag atatttcata aaatcacctc gtcgttgtac accataatta
    12601 tattatataa atatattttt ctattgcaaa caatgttggt gatataaaaa aagagttctc
    12661 aatttggttg agaaactcat cgcgttaagt atttgtaata agcatataaa aacaccttac
    12721 ttcttggtaa ggtgttagtt aaaatcaatg attcaaatct aagaattata gtttaacaac
    12781 gtttgcagct tgtggaccgc ggtcgccttc aactacttca aactcaacag cttgaccttc
    12841 ttctaatgat ttgtaaccat cttggttaat tgctgaaaaa tgtacgaata cgtcattttc
    12901 tccttcaact tcgataaagc cgaatccttt ttcagcgtta aaccatttaa ctgtaccttg
    12961 tttcataatc tgaaacctcc aagactaaaa ttcattcaat atgcttattc gcatattaca
    13021 aaaaatacat gcaaatttca ctcaaatatt ctttgcaata tggaataatc atctgcttaa
    13081 cttgtattat agtgcagtta ttaccaaatt gcaatacaat aataaaaaat attcatttaa
    13141 tcaattattg gtagtttata atatacttca ttattttgta aaacaatgaa actataaaat
    13201 atttgtaaat cctcttcaca atcttcgcaa tagttcatgt cacaattatt ataaaatgca
    13261 taaatattat atttgcctgt gacatagtta tcgaactgaa ctttacattg attaatcaat
    13321 tgttgatagt gcttaaacat gtcatcaaaa tcttgatatt gatatttttc aacaatgttt
    13381 tctggccagt cactgaataa ccaccatccc tcataatcgg ctctaatttt ggtaactgtc
    13441 cacattatta aagacccctt ccatacttca aaaactctat acttattttt atacctttat
    13501 cctctgaaaa tcaagtttga cgacttgaaa caaacaaagt agtaactata gtaattatta
    13561 gtttttttaa gtaaaactac gtataataac attatgaggt ggtaaaaatg agacatatac
    13621 atttacaagt attcggacgc gttcaaggcg tcggatttag atattttaca caacgcattg
    13681 caatgaacta taacattgtc ggtactgttc aaaatgtaga tgactatgta gagatatatg
    13741 cacaagggga tgacgcagat atagagagat ttattcaagg tgtaattgaa ggtgcctcac
    13801 cagcatcaaa tgtaacaagc catcaacttg aagagttaga actaaatcaa aaattatcgg
    13861 attttcgatc aatataaaat gctaaataaa ggaagatgac agtgagatat aatatttctc
    13921 atatatttgg ggtgttagtg ggaattcctg tagcgttttt aacaagcata tttgggatga
    13981 ttgcacttga tgtatctttt ttaattgata tgtctattgg tattgttggc tttttaatga
    14041 catacctacc gatacaaaaa ctcacttcac gcaaatattt aaacgaaatt ggtttgacta
    14101 gaaaagacta tcgctatatt cgaaatcagt taaatcatac acaccaaaaa cttagaggta
    14161 ttttaaaaac gtatgtcaat ataagatcaa ttaaagattt taggcagatt aatgatatat
    14221 accaaatttc acgttctatt tatacgacag ttagacagag acctgcatca ttttataaag
    14281 ttgaaggctt tttttattct catattgata atgctttaaa tttggttgat gcatatacac
    14341 gtctagcaaa aatgcccaaa aaatcaatta atgaacagca aaagttagaa caaacacgaa
    14401 ttactttgga tgaggtcaaa cgaacattaa tcgctgattt aaagcgtctc aacgaagatg
    14461 attatgaacg tttagatatt gaaatggaat taaataagtt acatcaaaaa catcatcaag
    14521 attgatgtaa aatagcaact attggagaga tgattgatga ctgaaaataa aagtttcaaa
    14581 gaaagccatc cactagatga ttttataagc gataaagaat tatcgaatac tactattcaa
    14641 aaagaaaagt taacaattga acaacaaaaa caggtagaca caatcagtaa acaaattaac
    14701 cctttagaca atgaaggttt attagcgttt ggttctgatt tacagaaaca aatgtctcaa
    14761 ttttcacatc aaatgttgga tgaagtacaa agtaaagatg ttggtcctat tggagatact
    14821 ttgtcagatc taatgtcaaa actaaagtca gttaatccaa atgagttaaa tactgataaa
    14881 ccatctatgt taaaaagaat ttttagcaga gcaaagtcgt ctatcaatga aatcttttca
    14941 agaatgcaat cagttagtgc tcaagtcgat cgcataacga ttcaactgca gaaacatcaa
    15001 acacatttaa caagagatat tgaattatta gatacgctat atgataaaaa caaacaatac
    15061 tttgatgact tatcattgca tatcattgct gcacagcaaa aaaagttgca attagaaaat
    15121 gaaaagctac cacaattgca acagcaagcg cagcaatcca ctaatcaaat ggatattcaa
    15181 caagttgcag atatgcagca atttatagat agactagata aacgcatata tgacttacag
    15241 ctttcaagac aaatagcttt gcaaactgcg ccacaaattc gtatgattca aaatgttaat
    15301 caagcacttg ccgagaagat acaaagttca attttgacaa gtattccact atggaaaaat
    15361 caaatggcca ttgcgcttac attaatgaga cagcgtaatg cagttgctgc acaacgagct
    15421 gtcactgata caactaatga tttattaaca gcaaatgctg aaatgttgaa acaaaatgcg
    15481 attgaaactg caacagaaaa tgagcgtggc attgttgatc ttgatacatt gaaacgtaca
    15541 cagcgtaaca ttattgagac aattgaagaa acattaatta ttcaacaaca cggtcgcgaa
    15601 gaacgacaat tagctgaaaa agaattacaa caattagaac aagatttaaa gtcacattta
    15661 gtgaacatca aaggaccgaa taaacaatca taaattacga attagatata acaaaaaaga
    15721 gcttgggaca ttaagttcct taaagttctt aggcaatgta aaaaagctga tttc
//
LOCUS       DAMQYT010000042        15621 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMQYT010000042 DAMQYT010000000
VERSION     DAMQYT010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15621
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.042"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            209..1192
                     /locus_tag="PGA33_001038"
     CDS             209..1192
                     /locus_tag="PGA33_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDG6472863.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            1383..2309
                     /gene="pstC"
                     /locus_tag="PGA33_001039"
     CDS             1383..2309
                     /gene="pstC"
                     /locus_tag="PGA33_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDG6472864.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            2311..3228
                     /gene="pstA"
                     /locus_tag="PGA33_001040"
     CDS             2311..3228
                     /gene="pstA"
                     /locus_tag="PGA33_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDG6472865.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            3275..4126
                     /locus_tag="PGA33_001041"
     CDS             3275..4126
                     /locus_tag="PGA33_001041"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDG6472866.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            4133..4774
                     /gene="phoU"
                     /locus_tag="PGA33_001042"
     CDS             4133..4774
                     /gene="phoU"
                     /locus_tag="PGA33_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDG6472867.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(4914..6728)
                     /gene="pepF"
                     /locus_tag="PGA33_001043"
     CDS             complement(4914..6728)
                     /gene="pepF"
                     /locus_tag="PGA33_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDG6472868.1"
                     /translation="MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
     gene            6933..7277
                     /locus_tag="PGA33_001044"
     CDS             6933..7277
                     /locus_tag="PGA33_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472869.1"
                     /translation="MTLFDMPNYLWITTLIMILLTIFCCLVLNKWFVSAVITFVILGV
                     LAFFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYG
                     YEGAELRRKDKK"
     gene            7225..7410
                     /locus_tag="PGA33_001045"
     CDS             7225..7410
                     /locus_tag="PGA33_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472870.1"
                     /translation="MKNTAMRALNFAVKIKNNIRQLYAQNHRLLSYTAVTIISKEMEY
                     SAIVVFVLTFLYFTWLT"
     gene            7581..8567
                     /locus_tag="PGA33_001046"
     CDS             7581..8567
                     /locus_tag="PGA33_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6472871.1"
                     /translation="MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKIKTNTPLISESSDRHA"
     gene            8560..9390
                     /locus_tag="PGA33_001047"
     CDS             8560..9390
                     /locus_tag="PGA33_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6472872.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITLIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            9377..10150
                     /locus_tag="PGA33_001048"
     CDS             9377..10150
                     /locus_tag="PGA33_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6472873.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENVSRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKGSKEEALSKVDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            10143..10844
                     /locus_tag="PGA33_001049"
     CDS             10143..10844
                     /locus_tag="PGA33_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6472874.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQAFSY"
     gene            10968..11441
                     /locus_tag="PGA33_001050"
     CDS             10968..11441
                     /locus_tag="PGA33_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDG6472875.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGN
                     KVYRCYIDMEHPRKSICNCPHADGRRVICKHMIALLFTASPEAANKHIMMLNEVEEDY
                     QLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEEEMFEDEFY"
     gene            complement(11489..12256)
                     /locus_tag="PGA33_001051"
     CDS             complement(11489..12256)
                     /locus_tag="PGA33_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDG6472876.1"
                     /translation="MRFVFDIDGTLCFDGRLIDQTIIDTLLQLQHDGHELIFASARPI
                     RDLLPVLPSVFHQHTLIGANGAMISQQSKISVIKPIHTDTYHHIFKIIQKYELDYIID
                     DDWNYAAQLDAENAIFERLDPHKLASCIDVANIDTPIKIILLNIDPAQITTILDELDK
                     YHQELEMIHHSNEYNIDITAQNINKYTALQYIFDADVKYIAFGNDHNDIVMLQHASSG
                     YIIGPSEAYTHAILKLDKIKHINNNAQAICKVLKSYK"
     gene            complement(12945..14204)
                     /gene="femB"
                     /locus_tag="PGA33_001052"
     CDS             complement(12945..14204)
                     /gene="femB"
                     /locus_tag="PGA33_001052"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HDG6472877.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
     gene            complement(14223..15485)
                     /gene="femA"
                     /locus_tag="PGA33_001053"
     CDS             complement(14223..15485)
                     /gene="femA"
                     /locus_tag="PGA33_001053"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HDG6472878.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
BASE COUNT         5321 a         2401 c         2530 g         5369 t
ORIGIN      
        1 tttaaatttc gatgattttt caggaagcat tttaatttta ctaatgaagc aatatttttt
       61 agattaacaa aaattaatat ttacattttc ttaacaattt tttatgtaac atttacagtt
      121 tctaaaaatg aggttaataa ttcaaggtta agataaagat gtaatcaata caaatactat
      181 ttgttgttca tacagggagg atatttcaat gaaaaaatgg caatttgttg gtactacagc
      241 tttaggtgca acactattat taggtgcttg tggtggcggt aatggtggca gtggtaatag
      301 tgatttaaaa ggggaagcta aaggtgatgg ctcatcaaca gtagcaccaa ttgtggagaa
      361 attaaatgaa aaatgggctc aagatcactc ggatgctaaa atctcagcag gacaagctgg
      421 tacaggtgct ggtttccaaa aattcattgc aggagatatc gacttcgctg atgcttctag
      481 accaattaaa gatgaagaga agcaaaaatt acaagataag aatatcaaat acaaagaatt
      541 caaaattgcg caagatggtg taacggttgc tgtaaataaa gaaaatgatt ttgtagatga
      601 attagacaaa cagcaattaa aagcaattta ttctggaaaa gctaaaacat ggaaagatgt
      661 taatagtaaa tggccagata aaaaaataaa tgctgtatca ccaaactcaa gtcatggtac
      721 ttatgacttc tttgaaaatg aagtaatgaa taaagaagat attaaagcag aaaaaaatgc
      781 tgatacaaat gctatcgttt cttctgtaac gaaaaacaaa gagggaatcg gatactttgg
      841 atataacttc tacgtacaaa ataaagataa attaaaagaa gttaaaatca aagatgaaaa
      901 tggtaaagca acagagccta cgaaaaaaac aattcaagat aactcttatg cattaagtag
      961 accattattc atttatgtaa atgaaaaagc attgaaagat aataaagtaa tgtcagaatt
     1021 tatcaaattc gtcttagaag ataaaggtaa agcagctgaa gaagctggat atgtagcagc
     1081 accagagaaa acatacaaat cacaattaga tgatttaaaa gcatttattg ataaaaatca
     1141 aaaatcagac gacaagaaat ctgatgataa aaagtctgaa gacaaaaaat aataagacgc
     1201 aatttcaaat gtgtcttgaa acatgatttt gatggtgaat cattatttag agtacaaagc
     1261 ttgatttatc gagacgctga ttttgacatt cagttagtct acaagcttat caacttaaaa
     1321 tagtggttca tcattatttt acaaatctaa ttattttggg agtaatagaa agaggtttga
     1381 ttatgacttc atctactaat gttaaagctt taatcgaaaa aaataataat aaaaaaggaa
     1441 agcataatga caaaattata ccagttattt tagccgcaat ttcagcgatt tccattttaa
     1501 caacactagg tatattaatc acattgcttt tagaaaccat cacttttttc accagaattc
     1561 caataactga atttctattt tctactactt ggaatcctac cggttcagac cctaagtttg
     1621 gtatctgggc attgataata gggactttaa aaatcacagt tattgcgact atatttgcag
     1681 ttccagtcgg gcttggtgca gcaatttatt taagtgaata tgcaagtgac cgtgcacgac
     1741 gcataattaa accgatatta gaaattttag caggaatacc aacaattgtg tttggtttct
     1801 ttgcattaac ctttgttaca ccagtattaa gatctttcat accaggtctt ggagagttta
     1861 atgctataag tcccggctta gttgtcggta ttatgattgt ccctctcatc acaagtttga
     1921 gtgaggatgc aatggcatct gtaccaaata aaattcgaga aggtgcctat ggacttggag
     1981 caactaaatt agaagtagca actaaagtcg tacttcccgc agcaacatca ggtattgtag
     2041 cttcaatcgt tctcgcgatt tcaagagcaa ttggagaaac gatgattgta tcattagcgg
     2101 caggtagttc gccaacagct tcattaagtt taacaagttc gattcaaaca atgactggat
     2161 atattgttga gatagcgaca ggtgatgcaa catttggatc aaatatttat tacagtattt
     2221 atgctgtagg gttcacacta tttatcttta ccttaatcat gaatttactt tctcagtgga
     2281 tttctaagcg ttttagggag gagtattaat atggaaacga cagataataa tagacaatca
     2341 ctcgtcgatc aacaacttgt ccaaaaacat ttatcatcca gaacggttaa aaataaagtg
     2401 ttcaaactca tatttttagc atgtacatta ttaggacttg tcgtacttat tgcgttgtta
     2461 actcaaacat tgattaaagg ggtaagtcat ttaaatttac agtttttcac taatttttct
     2521 tcttcaacac catctatggc tggcgttaaa ggcgcgttaa tcggttcact ttggttaatg
     2581 ttaagtatca ttccattatc aatcatccta ggaataggta cagctatata cttagaagaa
     2641 tatgcgaaaa acaacaaatt tactcagttt gttaaaatca gtatttccaa tttagctggt
     2701 gtaccatcag ttgtatttgg gttattaggt tatactttgt tcgttggtgg tgcagggatt
     2761 gaagccttga aaatgggtaa cagtatattg gcagcagcgc taacaatgac cttactgata
     2821 ttaccaatta ttattgtttc aagtcaggaa gcaattagag ctgtacctaa ctcagtacgc
     2881 gaagcttctt acggcttagg tgctaataaa tggcaaacga taagacgtgt tgtcttacca
     2941 gcagcgttac ctggtatttt aactggattc attttgtctc tttcaagagc actgggagaa
     3001 acagcgccac ttgtgctaat cggtataccg actatattat tggcaacacc tagaagtata
     3061 ttggatcaat tttcagcatt acctatccaa atatttactt gggcgaaaat gcctcaagaa
     3121 gaattccaga atgttgcatc ggcaggcatt atcgttttac tagttatctt aatcttaatg
     3181 aatggcgttg cgattatttt acgtaacaaa tttagtaaaa aattctaatt taaacaatca
     3241 atctcattta tctattaaaa agggagtttt aaatatggcg caaacacttg cacaaactaa
     3301 acaaatatct caaagtcata cgtttgatgt ctcacaaagt catcataaaa caccagatga
     3361 tacaaactca cattctgtta tatattcaac acaaaattta gacttatggt atggcgaaaa
     3421 tcatgcatta caaaatatta atttagatat ttatgaaaac caaattactg ccattatagg
     3481 tccatctggt tgtggcaaat caacatatat taaaactttg aatcgaatgg ttgagttagt
     3541 accttctgtg aaaacagctg gtaaaatatt atatcgagat caagacattt ttgatcaaaa
     3601 atattctaaa gaacaattac gtacaaatgt gggcatggtc tttcaacaac ctaatccatt
     3661 tccaaaatca atatacgata atattactta cggtccaaag attcacggta ttaaaaataa
     3721 aaaagttctt gatgaaatcg ttgagaaatc attacgtggc gctgcaattt gggatgaatt
     3781 aaaggatagg ttgcacacaa atgcatatag tttatccggt gggcaacaac aacgtgtttg
     3841 tatcgcgcgt tgtttagcaa ttgaacctga agtcatttta atggatgaac cgacatcagc
     3901 attagatcca atctcaacat taagagtaga agagttggtt caagaactaa aagaaaagta
     3961 tacaattatt atggttacac ataatatgca acaagcagct cgtgtatcag ataaaactgc
     4021 atttttctta aatggttatg tcaatgaata tgatgatact gataaaattt tctctaaccc
     4081 atcaaacaag aaaacagaag attatatttc aggaaggttt ggttgatata taatggcaat
     4141 aattagacaa cgatatcagg agcaacttga tgatttaata aaagaattac gtcggttagg
     4201 tgcaaatgtc tatgtgagta ttgaaaatgg tataaaatca ttaagtattg acgatagagg
     4261 ctttgcacga caaacagtta aaaacgataa acatatcaat caattaaatt atgatattaa
     4321 tgagcgagtt atcatgttaa ttacaaagca acagcccatt gcgagtgatt tgcgtatgat
     4381 gatttcttca ttaaaaatcg cctccgattt agaaagaata ggagataatg cctcgagtat
     4441 tgccaatatt cgattgcgta caaagattac agatgattat gtgttaaccc gtttaaagac
     4501 aatgggtaaa ttagctatgt taatgttaaa ggacttagat caagcattta aaaagaaaga
     4561 taccgtatta ataagagaaa taattgagcg tgatgaagat atcgatgact tatatagtca
     4621 tattattaac gcaacgtatc ttattgataa cgatccattt gtcgctgcac aagctcattt
     4681 agcagcaaga catttagaac gtattggtga tcatattatt aacatcgctg aaagtgttta
     4741 tttttattta acaggtacac attacgaaca ataacttaaa gttattacta taaaatccct
     4801 tacgataaat atatatttct attattcata aaccctcaaa aaaaccaaga ttctcacaat
     4861 tagtaatgtg aaaatcttgg tttatattgt tctactataa attgtctcgc atcttagtta
     4921 tttgcttgct caatttcatc tgttaatttt tcaacttcat cgactaaatc agaaatatat
     4981 tgaattgtag atttaagtgg ctgttctgta gtaatgtcta cacctgcaat gtttgcaagt
     5041 tcgacaggtg atacactacc accttttttc aatgtttcta accaagcatc aacagctggt
     5101 tggccttcat ttttaatctt ttgagaaacg acagttccga ttgttaagcc agcagaatac
     5161 gtatacgaat ataatcccat atagtaatga ggttgacgca tccatgttaa ttcagcaccc
     5221 tcagtcatgt ctactgcatc tccaaaaaat tgtttataaa catttagcat tatttcattt
     5281 aatgtcggcg catttaaaga ttcaccttga tctactttgt gatacacttc acgttgataa
     5341 gcagcttcta ataaatgggt aaccatatta tgataatatg ttctagataa aattgagcca
     5401 ataacccaac gcttaaatct tggattatca cttgtgttaa ataaataatt ggccatcaac
     5461 atttcattca ttgtagaagg ggcttcaaca aagtacattg atgcttctga ttcaagatat
     5521 ggttgatgtt tttgagctaa tgtaaaatga cctgcatgac ctaattcatg tgctaagaca
     5581 aatgcttcag ccattttacc agtccaagaa ataaacacat atgaatgtgt aaagtatgga
     5641 cttgcacaaa atgcgcctgt atctttacct ttattttgtg caaaatcaat ccatcgctga
     5701 tcgtatgctt cacgtaacat gtttgtatag tcatcaccta aaacacttaa cgcaccgaaa
     5761 atataatttt ttgagtcttc aattgaaatc tctggttcat aatcagggtc tacagaaatc
     5821 ttcaagtctt caaaacgcat gttatctaat ccgtgaatac gttgtaaaag tttagcatat
     5881 ttctgcataa ctggtgccaa gtcacgcata atcatatcga tttgacggtc aaacatatca
     5941 cgcgttactt cttgactatg taataaataa tcgatgactg attcaaatcc acgtaaatca
     6001 gcttcaattt tttcttgttg tacttgcata ttatatgtag ccgcggtagt atgctgatat
     6061 tttcgaatcc catcgctaaa cgatttgaaa cttttacgtc taaactcagg atctttatta
     6121 tcttcataat cattttcaaa cgtagcatag tcgacagggt acgttgtacc atcatgttca
     6181 aatgaatcga atgtaatatc tagcattttt gtcgtgccgt ataaatcgta aggactgttt
     6241 agcgtaggtg ataaagttgc taatacttgt tctaccgacg cagataattg gaatggcttt
     6301 tgttttatta actgtttaat atagtgttga tatggacatg attcttctaa ttgttgaagt
     6361 atttcttctg gtaattcaag tatttctgac tctacaaatg ataattggct aacaatttta
     6421 ccgtatgtag tagataattt agcgctcaat acttgtgcct cgatattact agtatctaca
     6481 cttaaacgta gttctgcata attacttaag cgatctaagg caattaaaat attttctaat
     6541 tcagctaaag cagtattaat ttgttcgatt gaatttaatg ttgttgcata tgtatgatga
     6601 aattgatttg cttgttgtac tagagcgtca atactttcat aatattgttg atcatcttta
     6661 aataagtcta ctaaatccca tgtttctgaa acaggaacat cttctcttaa aggtaaacct
     6721 tgagacatat aatcactcct ttggtataat acattcatta tacataattt tgtctcctaa
     6781 acgataatgt tacgatttcc atgtcgcaag gaacaatgat ttttctatta gtagatagaa
     6841 tattgcttga aactgactgc tattgtttaa gtttttaaaa atttataata aaatgtatac
     6901 aaggaaagta atgaaataaa aggagaatga atatgacatt attcgatatg cctaattatt
     6961 tatggatcac aacattaata atgattttat taacaatatt ctgttgctta gttttaaata
     7021 aatggtttgt atctgcagta attacatttg ttatattagg tgtgcttgca ttttttattc
     7081 caaattttca agatataaaa tatcaaccat tattaggata cgctgcattt ttagcgatta
     7141 tgagtttatt aattagtttt ctattatggt attttactag aaattggcgt aaagaaagaa
     7201 aagcgcgtaa attagaaaag caaattgaaa aatacggcta tgagggcgct gaacttcgcc
     7261 gtaaagataa aaaataatat tcgacaacta tatgcacaaa atcatcgact tttaagttac
     7321 actgcagtaa caattatttc gaaagagatg gaatacagtg ctatagttgt ttttgttttg
     7381 acttttttat attttacatg gttgacttga ttcaaaatat tatattttat aatgattttg
     7441 ttaaaaaatt aaatatactt caatttcttt atggataata ctataatgaa cgttagttct
     7501 taatatgtaa gttgcatata catgcgccat tctcatgtaa aaagaaagtg ttagtaactt
     7561 acacaaggag ggggactatt atgttcataa taaagtctat gttgtataga ctgatgcaaa
     7621 tgatagttgt tttatttgtt atttctacac ttacattcat tttaatgaaa ttatcaccag
     7681 gcaatcctgt tgataaaata ttacatcttg atgtagcaca agtgtcgacg gaacaaataa
     7741 atgcaaccaa agataaattg ggattaaatg attctttatt ggtacagtgg tggcattgga
     7801 tgaatcattt attacatttt aatttaggaa aaagttttga atcaaaagaa ccggtaacac
     7861 agatattatt caactatgca cccataacat tactcatatc attttcaaca ttagtagtgt
     7921 cgttatgcat atctataccg ctcggcatta tagcagccaa acgatttcat aagtggacgg
     7981 ataaagtgat tagagtgatc tcaacactat cgattagttt accagcgttc tttatcggta
     8041 ttattttatt atttattgtc acaaatttaa tgaatataga tagcgttata ctaagtcagt
     8101 ttatattacc tgtaatcacg ctatctttag gtatgtgtgc atacatcatt cgtttagtgc
     8161 gttctaattt attgatgtta ttgcaaagta atatcgtaca agcatcaaga ttacgcggta
     8221 tgaatgaacg ttatatttta attcatgatt tactaaaacc aacaattttg ccgattatcc
     8281 cattactagg gatttcactt ggcagtctaa taggtggtac tgtagtgatt gaaaatttat
     8341 ttgatatacc tggtattggt tatctattaa tggatagtat taaatctcga gattatcctg
     8401 ttattcaagg atgcgtgtta tttattggct tcttcgttgt tattatcaat acgattgctg
     8461 atttattaac gttattactt gatccgaagc agcgtttaca attaggaaat ccaaaaatca
     8521 aaaccaatac accattgata tcagaaagta gtgaccgtca tgcataaaat attttcaaag
     8581 aataacctga tattttttgt attcgttgca tttatttttg tggtaattgt actgcaattc
     8641 tttgtcagta gtgaaaatgc aaccaaagtc aatttatcac aaacttttga acctattagt
     8701 tggttgcatt tattaggaac tgatgattat gggagagatt tatttacccg aattattatc
     8761 ggtgcacgtt caacattgtt tgttactgtt ttaacattaa tagctatcgt tgtcataggt
     8821 gttacactag gtctatttgc cggatacaaa aaagggtgga ttgaacgatt agtgttaagg
     8881 tttattgatg ttggtctaag tattccagaa tttatcatca tgattgcttt agcaagtttt
     8941 tttcaaccat ctttatggaa tttagttatc tcaattacat taataaaatg gatgaattac
     9001 acaaggttga ctagaagtat agttaatagc gaaatgaata agccttatat aaaaatggca
     9061 caattatttc atgtaccaac aagaacaata ttaatacgtc atttaacacc taaaattata
     9121 ccggctatta tcgttttgat ggtcgttgat ttcggtaaaa tcattctata tataagttca
     9181 ctatcattta ttgggttagg tgcacaaccg ccaacaccag agtggggcgc tatgttgcaa
     9241 caaggtcgtg attttatttc gtctcatcca attatgttga ttgcacctgc ttcagtcatt
     9301 gctataacta ttttaatttt taatttaacc ggtgatgcac taagagatag attgctgaaa
     9361 caacggggtg aatatgatga gtctcattga tatacaaaat ttaacaataa agaatactag
     9421 tgagaaatct cttattaaag ggattgattt gaaaattttt agtcaacaga ttaatgcctt
     9481 gattggagag agcggcgctg gaaaaagttt gattgctaaa gctttacttg aatatttacc
     9541 atttgattta agctgcacgt atgattcgta ccaatttgat ggggaaaatg ttagtagatt
     9601 gagtcaatat tatggtcata caattggcta tatttctcaa aattatgcag aaagttttaa
     9661 cgaccatact aaattaggta aacagttaac tgcgatttat cgtaagcatt ataaaggtag
     9721 taaagaagag gctttgtcca aagttgataa ggctttgtcg tgggttaatt tacaaagcaa
     9781 agatatatta aataaatata gtttccaact ttctgggggc caacttgaac gcgtatacat
     9841 agcaagcgtt ctcatgttgg agcctaaatt aatcattgca gacgaaccag ttgcatcatt
     9901 ggatgctttg aacggtaatc aagtgatgga tttattacag catattgtat tagaacatgg
     9961 tcaaacatta tttattatca cacataactt aagtcatgta ttgaaatatt gtcagtacat
    10021 ttatgtttta aaagaaggtc aaatcattga acgaggtaat attaatcatt tcaagtatga
    10081 gcatttgcat ccgtatactg aacgtctaat taaatataga acacaattaa agagggatta
    10141 ctatgattga gttaaaacat gtgacttttg gttataataa aaagcagatg gtgctacaag
    10201 atatcaatat tactatacct gatggagaaa atgttggtat tttaggcgaa agtggctgtg
    10261 gtaaaagtac gctcgcttca ttggttcttg gcttgtttaa acctgttaaa ggagagattt
    10321 acttaagtga caatgctgtg ttaccgattt tccaacaccc tttaactagc tttaaccctg
    10381 attggacgat tgagacctca ttaaaagaag cgttatatta ttacagaggt ctaactgata
    10441 atactgctca ggatcaatta ttattacaac atttatctac ttttgagtta aacgcgcaat
    10501 tattgactaa attaccaagc gaagtgagtg gcggacaatt acaaagattt aatgtcatgc
    10561 gttcgttatt agcacagcct cgcgttttaa tatgtgatga gataacttca aatttagatg
    10621 ttatagctga acaaaatgta atcaatatat taaaagcgca aacgattacg aacttaaatc
    10681 attttatcgt tatttctcat gatttatccg tgttacaacg cttagttaat agaattatcg
    10741 ttcttaagga tggcatgata gtcgatgatt ttgcaataga ggaattattt aatgttgata
    10801 gacaccctta tacaaaagaa ttagtgcaag cattttcata ttagttattt aagaatgcga
    10861 taattctaga cttgttataa aatatagata aatcaagtat tttaatctag acacttatct
    10921 attttatttt ctttatttaa aaataataat aaaaaggagt atcattaatg ggattacttg
    10981 atattgcaag tattcgttct atagaaagag gctttaatta ttatcaaagt gaatgcgtca
    11041 ttaacttaaa atcattttca gaaacgcagc atgaggctga agtaaagggc agtggcaaca
    11101 aagtatatcg ttgttatatt gatatggaac atcctagaaa atccatatgt aattgtcctc
    11161 atgctgatgg aagacgagtg atatgtaaac atatgattgc attactcttt acagctagtc
    11221 cagaagcagc aaataaacat ataatgatgt taaacgaagt tgaagaagac tatcaattac
    11281 gcagaaatat gtggattgat agtcttaaag aaatgattaa tgatatgagt gaagaagaac
    11341 tccgcgatgc atatttaaac atgttaattg aacatggaga aatggcagaa ttatttggat
    11401 tagatgaaga ggaagaaatg ttcgaggacg aattttatta aaatagcccc tatcgattga
    11461 taatgattat catttgatag gggtgttttt atttatatga ttttaagact ttgcaaatag
    11521 cttgtgcatt attattgatg tgtttgattt tatcaagttt caatattgcg tgtgtgtatg
    11581 cttctgatgg tcctataata tagccactac tagcatgttg taacatgaca atatcattgt
    11641 ggtcattacc aaatgctata tatttaacat ctgcatcaaa tatatattgt aatgcagtat
    11701 atttgttaat attttgcgct gttatatcaa tgttatactc atttgaatgg tgaatcattt
    11761 ccaattcttg atggtattta tctagctcgt ctaatatagt tgtaatttgt gccgggtcta
    11821 tatttaataa aataatcttg attggtgtgt cgatatttgc aacatcaata caactggcca
    11881 gcttatgtgg atctaaacgc tcaaaaatcg cgttctcagc gtcaagttgt gcagcataat
    11941 tccaatcatc atcaataata taatctaact catacttttg aattattttg aagatatgat
    12001 gatatgtatc agtatgaatt ggtttgataa cagaaatctt tgattgctgt gaaatcatag
    12061 caccatttgc gccaattaat gtgtgctgat gaaatactga tggtaaaact ggcaacaaat
    12121 cacgaatcgg acgtgctgat gcaaatataa gttcatgacc atcatgttgt aattgtaaca
    12181 atgtatcaat aatagtctgg tcaattaatc ggccgtcaaa acaaagcgta ccatcaatat
    12241 caaagacaaa tctcatcaaa tcactccaaa caatataata ccatgattat agcataagta
    12301 agttatgtga cgatattaga taatgattat gatgtagggt accttttgcg ttacacacat
    12361 ataacttcct ataatatata ttatgtaaac tagaatgtga aattccataa ggggacttat
    12421 ataactgtat gtttgtgtag tgtttatgtc agtaagctaa atttacattc atgttatgtt
    12481 tcattaaacc aattactcac gttatcattt tggtgcatat taaatctttt atatcgatca
    12541 tacatctatc atcattttta tttctaaaat acaaactgaa tactttgcta gaatttgtta
    12601 cagcaatcat tgctcatgca ttttataaat tacaattaga caaatatgac atttgatatc
    12661 acacacttgc aaacacacac atatataatc agacataaat tgttatgcta aggtttattc
    12721 accaaaaata taatacatat tggcttgttt tgagtcatat tgaatgaatt aaaaagtata
    12781 ctcaactcaa tcatttacaa ataggttgtg caattcatat ttatatttat tgcaatcact
    12841 tgttaaactt gtcatttaat atatcattat tcgaggcttt attaaaaaac atataaaaaa
    12901 gagcctatag tatcaataac tataagccct aacatcattt acatctattt ctttaatttt
    12961 ttacgtaatt tatccaatgt tgtgaacaac caatatttca ctttatgaat tggtttatag
    13021 aaatccccta ttaattcttc gatttgtaca ttaaatccac gtttaaagcg gtatacgcca
    13081 taatcttcac tgttttccgt aaaatcacct gataaaccat agaaattata acgatcatag
    13141 ccattatcga agcaatagtt aatcataaac caatgcatca tgtatggtcc cataaattga
    13201 ttatattttt ctgatgaacc accagagaaa taattcactt catatgcatt tgcaaaataa
    13261 acgccagaag caaggtttag aattgggccg tccgttttgc tcaattcact tgcattcaat
    13321 aattcatgct gatcatgatc aatttgctta tctaattctg caattttctt catttgctta
    13381 tctgatttgt tttctttcgc catcatttga tcacgacgat tttctttgtc attcaattcc
    13441 tgttgcaact ttaacacata ttcatcaagg tcaatatatg ctaatggtac taatacttta
    13501 tctccatatg tgtcaataaa gttataaaaa taatcatctg tttttgatac aaatccagca
    13561 cgctcttcag tttcacgata taaatctaag aaaagattga actcatcacg ttcaaggaat
    13621 ctgactttaa caccatagtt tatcgcttta ttaatattac gtttacgttg actatcaaat
    13681 gtctttttca atgtttcggg tgttttacct tcaaggttta atacgcccat ccatcgtact
    13741 tggctcgatg tatcatactc agttgtaaag ccatgatgct cgtaaccatg tgatttaaac
    13801 aagtttacta gggcatcatt tttctcgcga ccttcaaatg gcacgatatc tttatcatat
    13861 agatgatata accaatacgg atctaattta acatataaac attgatgttg ctgtaaatat
    13921 ttatctaact cttttaaata ataatcaact aatcctaaat ctgaaaaatc cattactgga
    13981 ccacgattcg aatagtaaac ataacttccc atagtaggaa ttttagagaa aaggcttgct
    14041 gcaattactt tgttattgtc gtctttaata cctaataaaa ctacttcaaa gccatcattc
    14101 tcacgggtaa ctatattttc ttttacttgg aaataatgac tttccaatga tggattttgt
    14161 acaaagttgt caaattcggt aacagttaac tctgtaaatt tcatgttttg ataattccct
    14221 tcctaaaaaa ttctgtcttt aactttttta agtgcggtat atgctgcgta aacaggttta
    14281 ttaattggtt taataaagtc accaacatat tcaataattt cagcattgta accttttttg
    14341 aatttaacta caccagcatc ttcagcatct tctgtaaatt taccactaac accatagaaa
    14401 ttataacggt caatgccatg atttaatgca taattaatca tttcccattg cactgcataa
    14461 cttccggcaa aatgacggaa tgcatttgat gtaccaccag cataataaac aacttcaaat
    14521 ggattgataa agaagaaacc agcagagata ggtaattcat taccatgttc ttcttgtaga
    14581 cgtttacctt cttcaatctt ttgctcattt gcatcaagtt gttgttgtaa gttatctcgc
    14641 ttgttatgtg cttttttatt ttcaggacgt ttttcaatat cctttaacgc tttatttaaa
    14701 tctttattta aaatatcacg ctcttcgttt agttctttaa tatattcatc aaagttgata
    14761 tacgctaaag gtactaacac acggtctttg taatatttta agcgattgta gtaaaatttg
    14821 tcatcacgat cagcaaaagc ttttgattct gacgtatctt ccataaatga tctaaaaatt
    14881 ggtagttctt cttcagataa aaatcttact ttaacaccat tctttttaac ttttttcgtg
    14941 tttctttttc taagtccatc catattttta atgatgtcat ctgctgtttt atcttttaaa
    15001 tctaacactg agtgataacg aatttgtagc acaggatcaa atcctttatg gaatccagta
    15061 tgttcaaatc ctaagttact cattttatca aagaaccaat cattaccagc attacctgta
    15121 atctcgccat catgattcaa gtattgatat ggtaaatatg gatcgatatg taggtataga
    15181 caacgatgtt ttttaacata ttttgataat tcattaaaga aaaagtgtac gagttcttga
    15241 ttttcataat caatcactgg accgcgattt gaataaaaat acttgaacac tttcataaca
    15301 ggtacagcag taagtaagca agctgcaatg acctcgttat tattgttttt tattcccact
    15361 aaatgtgttt cataaccttc agcaagcttt aactcatagt ggccaacagt ttgcgtgaaa
    15421 tgactgtatg gcatgctatc tgtaaaggca ccaaactctt tagctgttaa atttgtaaac
    15481 ttcattatca ttactcctat ttgtctctcg ttaattaatt tcatttccgt atttgcagtt
    15541 tttctatttc ccctctgcaa atgtcaaaaa taataaatct aatctaaata agtatacaat
    15601 agttaatgtt aaaactaaaa c
//
LOCUS       DAMQYT010000043        15454 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMQYT010000043 DAMQYT010000000
VERSION     DAMQYT010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15454
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.043"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            143..592
                     /locus_tag="PGA33_001054"
     CDS             143..592
                     /locus_tag="PGA33_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HDG6472879.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWMLHGQDYTAIQQ"
     gene            complement(673..1260)
                     /locus_tag="PGA33_001055"
     CDS             complement(673..1260)
                     /locus_tag="PGA33_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDG6472880.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSFI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(1238..1336)
                     /locus_tag="PGA33_001056"
     CDS             complement(1238..1336)
                     /locus_tag="PGA33_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472881.1"
                     /translation="MPIINVGDLWDNEHNVYNHWGGLDINDYRSTY"
     gene            1393..3075
                     /locus_tag="PGA33_001057"
     CDS             1393..3075
                     /locus_tag="PGA33_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDG6472882.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGTIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKQQD
                     VSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN"
     gene            complement(3204..3587)
                     /locus_tag="PGA33_001058"
     CDS             complement(3204..3587)
                     /locus_tag="PGA33_001058"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDG6472883.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(3589..4440)
                     /locus_tag="PGA33_001059"
     CDS             complement(3589..4440)
                     /locus_tag="PGA33_001059"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDG6472884.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITIVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            complement(4433..5251)
                     /gene="panB"
                     /locus_tag="PGA33_001060"
     CDS             complement(4433..5251)
                     /gene="panB"
                     /locus_tag="PGA33_001060"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDG6472885.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            5324..6184
                     /locus_tag="PGA33_001061"
     CDS             5324..6184
                     /locus_tag="PGA33_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDG6472886.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HISFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIAHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            complement(6270..6974)
                     /gene="budA"
                     /locus_tag="PGA33_001062"
     CDS             complement(6270..6974)
                     /gene="budA"
                     /locus_tag="PGA33_001062"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDG6472887.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(7252..8211)
                     /locus_tag="PGA33_001063"
     CDS             complement(7252..8211)
                     /locus_tag="PGA33_001063"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDG6472888.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
     gene            8898..10346
                     /locus_tag="PGA33_001064"
     CDS             8898..10346
                     /locus_tag="PGA33_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDG6472889.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAITFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            complement(10427..11764)
                     /locus_tag="PGA33_001065"
     CDS             complement(10427..11764)
                     /locus_tag="PGA33_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDG6472890.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEIAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            12022..12438
                     /locus_tag="PGA33_001066"
     CDS             12022..12438
                     /locus_tag="PGA33_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472891.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPYILEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            12561..13451
                     /locus_tag="PGA33_001067"
     CDS             12561..13451
                     /locus_tag="PGA33_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HDG6472892.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVESKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDNLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNAELIASFSRALASDLRADQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(13644..15140)
                     /gene="mqo"
                     /locus_tag="PGA33_001068"
     CDS             complement(13644..15140)
                     /gene="mqo"
                     /locus_tag="PGA33_001068"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDG6472893.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
BASE COUNT         4851 a         2853 c         2486 g         5264 t
ORIGIN      
        1 cttcatttaa atcaatttca tcttataatt tatcgtgtat tttacaaaag attgacttca
       61 attcatcgta aaagttatac ttttgccatt ttttaatgta acatggtgtt agtaataaaa
      121 ataatacatt gaggtgtttt acatgacagc attattccct tatatcgctt ttgaaaattc
      181 aaaagaagcc cttgcatatt acgaagaagt atttggtgca actgacgtta aacgtttaga
      241 agttggcgaa gaacaagcgt cacattttgg tatgactaag gaagaagcgc aagaagcaac
      301 tatgcatgct gaatttgaag tgcttggcgt aaaagtgtta tgttctgatt cttttggtcg
      361 cgctgacaaa attaataatg gcatatcatt attaattgat tatgatgtta acaataagga
      421 agatgctgat aaagttgaag cattctatga gcaaattaaa gatcattctt caattgaaat
      481 agaattaccg tttgctgacc aattctgggg tggcaaaatg ggcgtcttta ccgataaata
      541 cggtgttcgt tggatgttac atggtcaaga ctatactgca atccaacaat aatgttattg
      601 ataaagatat catttaataa gcttatgtta tttagcaagg caagccactc tcgattcatg
      661 gcttgcctta ttttattgta tatttttaat tatcgttgct ggcccttgat tgacaatcgt
      721 attaaaatgc cttattaagt caactttgtc tatacggttt ggatcttcta cccaatgtct
      781 tataaaagac aatcccgcac ctgaaacata actcatgaaa taagaaaatg gtataccatt
      841 aatttgatca ttttcatttt tataacgttg taaatgacac ataattaaat cataaagttt
      901 ttcgtggata tttgaatctt tacccatacg aaacatcaat tgataaaatg cgatgtcttt
      961 ttctatcatt tctattaaaa cggtcataat ttgatgtatg ttatccgtgg ataacttaac
     1021 tgctccattt aacttctcat catgaatgaa gtctcttatt tcctccaact gctggtcctc
     1081 taatttttca agcaaatcat acttatcata ataatgcgta taaaatgtac tacggttaac
     1141 atcagctaaa tctgcaattt gttgcacagt aatctcttct aattggtgtt gatgtaaaag
     1201 ttcaataaat gcatttctca ttgcaacttg tgattttcta atacgtcgat ctatagtcat
     1261 ttatatcaag tcctccccaa tgattataaa cgttatgttc attatcccac aaatctccaa
     1321 cattgatgat tggcacacaa tgtttacctg tttaatatag gtgatacaaa caaacagaaa
     1381 aaggtgataa caatgaacca acatttacta ggaaatccaa aattaactgt aactcatgtc
     1441 aatgaagtta aagccggtat taaccacatc gttgtcgaca gtgttcaata tggaaatcaa
     1501 gaaatgatta tggaaaaaga tgtcactgtg gaaatgcgcg atggcgaaaa attatatatt
     1561 aatattttca gaccaaataa agatggcaaa ttccctgtag ttatgtctgc agatacttac
     1621 ggtaaagata ataagcctaa aatcacaaat atgggtgccc tttggccaac attaggtacc
     1681 attccgacat ctagttttac acctgaagaa tcaccagacc caggattttg ggtgccaaat
     1741 gattatgttg tagttaaagt tgcattacgc ggtagtgaca aatccaaagg cgtcttatct
     1801 ccatggtcaa aaagagaagc ggaagattat tacgaagtga ttgaatgggc agcaaatcag
     1861 tcatggagta atggaaatat cgggacaaat ggtgtttctt atcttgcggt gactcaatgg
     1921 tgggtcgcat cattaaatcc accacattta aaagcaatga ttccttggga aggcttaaat
     1981 gatatgtata gagaagtagc ctttcacgga ggtataccag atactggctt ttatcgtttc
     2041 tggactcaag gtatttttgc gagatggaca gataatccaa atatcgaaga tttgattcaa
     2101 gcacaacaag aacatcctct gttcgatgat ttttggaaac agcgtcaagt gccattatca
     2161 caaattaaaa cacctctact aacatgtgct agttggtcta cacaaggttt gcacaaccgt
     2221 ggctcttttg aaggatttaa acaagctgca tctgaagaaa aatggctata tgtgcatgga
     2281 cgtaaagagt gggaaagtta ctacgctaga gaaaatctcg aacgccaaaa atcattcttt
     2341 gatttttacc ttaaagaaga aaataacgat tggaaagata cgcctcatgt catttatgaa
     2401 gttagagatc aattttataa aggcgaattc aaatcagcgt cagcgttccc tttacctaac
     2461 gcagaatata caccattgta tttgaatgct gaaaatcaca cattgaatca tgcaaagatt
     2521 agtagcgcgc atgtcgcaca atatgactct gaagataaac aacaagatgt aagttttaaa
     2581 tatacgtttg acaaagatac tgagttagtt ggaaacatga acttaaaact atgggtaagc
     2641 actaaagact cagatgatat ggatttattt gcaggtatta aaaagttaga tcgtcgtggt
     2701 aatgaagtta acttccctga ttttaatcat attgaaaatg gtcaagtagc tactggttgg
     2761 ttacgcgtat cacatcgtga attagatcaa gaaaaatcct caatcgcgca accttggcat
     2821 aaacatgaaa cagaattaaa gttgtcacaa gatgagattg tacctgttga aatcgaattg
     2881 ttaccttcag gcacgctatt taaacaaggc gaaacattgg aagttgttgt aaagggtagt
     2941 gaaattgtaa ttggtaatag tactcctggc atgaaaacac gttatgaaca tgaagaaacc
     3001 gtaaataaag gcatgcacat gatttatact ggtggtaaat atgattcaca attaatcatt
     3061 cctatcgtta attgatatgc agcaattacg gtcgcttttg attaaaagtg acatagtgat
     3121 aggactgtat aacaagagaa agccacacgc ttggaatctt aaaccaaggt gtggcccttt
     3181 ttattattga tggctattta attttataac actatcgtat tttctttttc atgaatcatt
     3241 tcaataatga cattatcttc attcattact gctactttag gtgcatggtt tttaatttct
     3301 tcttcattca actgtgcata agtcatgatt atgactacat cgcctacttc aacaagtctt
     3361 gacgctgcac cgtttaaaca aattttacca ctacctcttt caccagctat tacgtatgtt
     3421 tcaaaacgtg caccattatt attattcacg atggctactt tttcatttgg caagatgtct
     3481 accgcttcca atatatctga atcaatcgta atgctaccta cataatttaa atttgactca
     3541 gtcactcttg ctctatgaat tttagcattc atcattgttc ttatcacttt attcagctcc
     3601 aattattata ttatctatta aacgcgcttt tgaaaattta acagctaacg agataaatat
     3661 gcgtccagtt atttcgtgtt gttctactaa ttgaggataa ctataaacag caacttcttc
     3721 aatgcgtcca cttatatgtg attcaagata ttcagtaacc ctgtctataa ttactttact
     3781 ttgacgttca ccgtcttgat acaacgcttg tgctaatagc aaacttttac ttaaatgtac
     3841 cgcttcttgt cgttcttgct ccgttaaata aacatttctt gaacttttcg ccaaaccatc
     3901 tgcttctcga acgatatcaa taccaataat ttcaacggca tgattgaagt cttttaccat
     3961 ttgctcgaca atagccaatt gctgggcatc ttttttacca aaataagcat aatccggcat
     4021 aacaatatta aatagcttat taactactgt taccacccca tcaaaatgcc ctggacgctt
     4081 cgctccttct aacacatcag ctaatgggcc tactttgaca tcaataccta attcacctgg
     4141 atacatatct tctactgcag gatgaaaaac aatgtccgct cctacttctg atactaattc
     4201 taaatcttta tcaatttgtc tcggataagc atcgaaatct tcgtttggac caaattgtaa
     4261 tggattaaca aatacactca caattgtaat atcatttgta ctaactgatt cgcgtaccat
     4321 cgttaaatgt ccatcatgta aggcacccat tgttgggata aaaccaatcg ttgtgcctga
     4381 gcgtttggct gctttaacaa tgtgttgcat ctcttttacc gtagtaatca gcttagtcat
     4441 tgttattaac ctcattcata attttctttt tatacgtata ttcttctgat ggaaatgcac
     4501 cagatttaac ttcttgatcg tattgtttta aaccatccac accaacacta aaatcagcaa
     4561 attgcttcac aaatttcgct ttatgttcaa caccataatt taacatatcg tgataaacca
     4621 atacttgacc atctgtacct tttcctgcac caataccaat gactggaatt gttaagtgct
     4681 tgctaatttc ttctgctaaa tcatttggaa ttgcttcaag tactaacgca actgcaccag
     4741 cttgttctac atttttcgcg tctaaaataa gttgctccgc tgcttctttc gttgcacctt
     4801 gtaatttata ccccataacg ccaacacttt gaggtgttaa tcctaaatgt gcaacaacag
     4861 gaataccaat tgccgttgct ttttcaataa atggtgtaat atgcgctcct tctgctttaa
     4921 ttgcatttgc attcgtctcc tgataaagct ttagagcatg atttaagtct tgtgtcatag
     4981 agatgcctac tgcaccaatc ggcatatcaa caactacaaa tgtatttggt gcgcctcttc
     5041 ttactgcacg accgtgatga atcatatctg ctaacgtcac ttgtacggta ctttcataac
     5101 ctaatacagt cataccaagt gaatccccaa caagaatcat atcaataccc gctgcttcca
     5161 cttgtttagc acttggaaaa tcataagctg ttaccataga aattttagtt tgcttttgtt
     5221 tcatatctat taattgactt actgttttca atgttattca acctcttttt gcagtattat
     5281 tagaaatagt ataatgaatc ttttatagaa aggaaagaag aagatgttat cagttgcgat
     5341 tatcggccca ggcgctgtcg gtacaactat tgcctatgaa ttacaacaat cattgcctca
     5401 tacaacgctt atcgggagac acgccaaaac aataacatat tatactgtac cacatgcacc
     5461 tgcacaagat attgttgtga aaggttatga agatgtcaca aacacatttg atgttattat
     5521 tattgcagtc aaaacacatc aacttgatgc agttattcct catttaactc atcttgcaca
     5581 tgaggacacg ctcatcattt tagcccaaaa tggttatggt caactcgaac atatttcatt
     5641 taaaaacgta tgccaagcag ttgtctatat aagtggtcaa aagaaaggcg atgttgttac
     5701 gcacttcaga gattatcaat tacgtataca agataatgca ttaactcgac aatttagaga
     5761 tttggtccaa gacagtcaaa tcgacattgt attagaagca aatattcaac aagctatttg
     5821 gtataaattg ttagttaact tgggcattaa ttctatcaca gcactcggta gacagaccgt
     5881 tgcaatcatg cataatcctg aaatacgtac actgtgtaga cagctattac tagatggttg
     5941 tcgtgttgca caggcagagg ggttaaactt ttcagaacaa actgtcgata ctattatgac
     6001 tatatatcaa ggttatcccg acgaaatggg aacgagcatg tattacgaca ttgcgcatca
     6061 gcaacctctt gaagtcgaag cgattcaagg ctttatttat cgacgtgcac gtgaacacaa
     6121 tcttgacacc ccatatcttg atactattta tagcttttta cgcgcgtatc aacaaaatat
     6181 gtaagcacat atataatata aaaagacctt aacgatgcat ttagaaccac ctgttcctaa
     6241 tatcgctaag gtcttttaat tatattagac tattcagctt ctctaatttc ttctgccaca
     6301 tctttatagt ctattttcgc tttaacaaac gtctcgttat ttaccgggaa atgttgttgg
     6361 aatgtttcaa agttttgtat ctcaacgaca acgtcatcca cttcaaagtc aagaacatgt
     6421 ccaccataag ctctttcatc atccgcaaaa tgtatatgaa aaccagcaga ccctacgcca
     6481 tgaaataatt ctggtgtaaa aaatccaaca atggcaccac gaatatcttg tcttttttcc
     6541 tcaggttgtc tgcgtgctga atcaatcaaa cgtgtatatg gcggttgctg agcaggcatc
     6601 attcgtacat gcatatgttt aaatgtgcca taaattttaa cagccgaaaa taaattctca
     6661 cttaacattt catttttaat ttgtgcaaat acatcatctt gtgataattg ttgcaatgga
     6721 aatgtcttac tcgctttaaa attagtaatc gatgcatacg gtactttctc atcgcctttt
     6781 aattctataa attctttatg ctcgttagca tgatatgcct ttccgtctaa aaatattact
     6841 tcgccatcag accctgttaa cgttgcaatc cctaaattcc catgttctaa caattcatta
     6901 attgtagctg tgccttctag taagccagcc attaacgtac ctaatgtacc atgttgatac
     6961 aagacattcg tcattccaaa accatctccc tatagttatt cctcgttgtc atgctctact
     7021 tcgtgattaa tgcagaacta ctttccttta tactcaatga cgcctcaaca tttttattca
     7081 tgtcactaaa ttttcacgag tcataatact ttattcaatt attattttac gcaaaagtat
     7141 tgcctctatc aatgcttgct aaaaaaatgg acctatcagt gccacgtgtt actgcaacac
     7201 taatagatcc tcttaccatt aaaatatttc taattaatac ttaaaatagg cttagtcttc
     7261 taataaatat ttaattgaat caaatgtatc ttctaatgtt ttaacagatt tatcgaacaa
     7321 cgcttgttct tcggcactta atggcatttc ataaatttta actgcgccat gttggttaac
     7381 taatgttggt acacctaggt aaacgccttt gtgaccacca tattgaccat ctaattgtat
     7441 agaaacattt aagacattat tttcattatt taaaatggct tttgaaatgc gcatcaatgc
     7501 taatgcaata ccatagtatg ttgacccttt agcttggata atttcataag cagcgtcacg
     7561 tgtattcaca taaatttctt ccgctttagc ttcgctacca gtttgttctt ttaatgtgtc
     7621 atatactgaa atacctgcta catttgcttg tgaccaaact gcaagttcag tatcaccatg
     7681 ctcgccaata atactagcgt caacacttga aggtgcaaca ccaagttctt ggctaattaa
     7741 atattgtaaa cgtgcactgt ccaatacagt acctgaaccg ataacacgct ctgctggtaa
     7801 tccagtatat tcttttacaa atcttgttaa aatgtctaca gggtttgccg cgattaagaa
     7861 atatccatca aagccactat ccataacact cttaacgatg ctcttcataa tcttagtatt
     7921 tttttcaact aattgtaaac gtgtttcacc tggcttttga ggtgcaccag ctgtaataac
     7981 aactaaatct gcatctttac agtcttcgta ttcacctgct ttcacatcaa ctggtgaagg
     8041 actgtggact gtaccatggt ttaaatcttg aacatctgct tttactttgt cttttgcaat
     8101 gtcaataatt acaaattcat cagcaacacc ttgcgtaacc attgcaaagg catagcttga
     8161 tcctacagat ccatctccga ttaatacaac ctttttacca aatgttttca taattatgtc
     8221 tcctaaccta attgaattta ttttacacgt gactacacgt aaaaatatct atactgaaat
     8281 taaagattaa caattttatt ttattataat attatgctac caaattacaa acataaaact
     8341 gagatgtaag cggagtcaaa ttacactcta tcgccaataa atattgatat ctcaagcttt
     8401 agcacgtaat ttgaaatttg tgacaatttt tagcattcta aaattgcgta acgacgctat
     8461 ttaaaggctt taacaaacta ttgagctttc tatttttttc attttaaaaa ggatggctat
     8521 tcgacttaaa tgacttacta tataacctcc tattttccgc cttaccctca tcatatttga
     8581 tgcatcattt tatagtcaaa gaattacatt tcatacaaac tagccaatca gacataaaca
     8641 tgaaattgat tttaaaaata taatgtgcaa aattgttaga aaattagcaa aaaatagtac
     8701 ttattcattt aatatctgtt ataatcagtt ttatcattta aaaaatctat ggatgttatc
     8761 aagacaagtc attaatacgt aacataaagt tgtgattttt cagaattttt agacaaaata
     8821 ttgattattt gtgctatgat ataacatatt aaaaaacacc gtatagaatt gtttatgaat
     8881 gtttgggagg aaagtttatg ggaagttttt tcaataaaat agcacgaaaa gaggatccgg
     8941 ctatctatca aaataaagat ggtcatttaa agcgtacact tcgggtgcgt gatttcttag
     9001 ctttaggtgt aggaacaatt gtatcgacat ctatctttac gctacctggc attgttgctg
     9061 cagaacatgc aggaccggcc gttgcgttat cattcttact cgctgctatt gttgctggtt
     9121 tagttgcatt tacttatgca gaaatggctg ccgctatgcc atttgcaggt tcagcctatt
     9181 cttgggtcaa tgtattattt ggtgaatttt ttggatgggt tgccggttgg gctctattag
     9241 ctgaatattt tatcgccgta gcctttgttg catcaggatt ctcagcgaat ttacgcggac
     9301 ttgtgaaacc aattggcatc gaattacctg cagcattatc aaatccattt ggtacaaatg
     9361 gcggttttat cgatattatt gctgctatcg ttattttatt aactgcatta ttactatcac
     9421 gtggtatgtc ggaagcagct cgtatggaaa atattttagt tattttaaaa gtattagcta
     9481 ttattttatt tgtcatcgta ggtttaacag caataaatgt tagtaactat gtgccattta
     9541 ttccagaaca caaagtaact gctacaggtg actttggtgg atggcaaggc atatatgctg
     9601 gtgtttcaat gattttctta gcgtatatcg gtttcgattc tatcgcagca aactcagcag
     9661 aagcacttga tcctcaaaag acaatgccta gaggtattct tggttcttta agcgttgcta
     9721 tcgtattatt tattgctgta gcacttgtgt tagttggtat gttccattac tcacaatacg
     9781 caaacaatgc tgaacctgtt ggttgggctt tacgtcaaag tggtcatggt gttgtagcag
     9841 ctattgttca agctatctct gttatcggta tgtttacagc attaattggt atgatgttag
     9901 caggctcacg tttactttat tcatttggac gtgacggctt attaccttca tggttaagcc
     9961 acttaaacga caaacattta cctaatcgcg cactagttat acttactatt attggtgttt
    10021 taattggttc tatgttccca ttcgcatttt tagcacaatt aatttcagca ggtacacttg
    10081 ttgcatttat gttcgtttca ttagcaatgt atcgtttgag aaagcgtgaa ggtaaagatc
    10141 taccaattcc tgcatttaaa ttacctttat atcctgtgtt accagcaatt acatttgttt
    10201 tagtattgct agtattctgg ggattaggat tcgaagcgaa attatacact ttaatttggt
    10261 ttattgttgg tatcatccta tatttaagtt atggacttag acattctaag aaaaatgacg
    10321 tagctgaata ccacccacca aaataaaaaa taaaaaaaat catttcccat ctgattaaca
    10381 tccagatgtg gaaatgattt tttattttat attaagataa tcgctattac caaccttgtc
    10441 cagatatgtc atattgatct aagttacctg cttccaaagc agtcagtgca tcttctatag
    10501 tgtttaacgc cgtgtctaat tgctcatatg ttattaccaa tggcggttgg aatcgcaaca
    10561 catttcctgc tacagctata ataactacgc catgctcaaa gcagtaatta caaattttaa
    10621 gtgccgcact ggcatcacgt gttttgagtt ttttgtcgga aacaatatca ataccaatgc
    10681 tcagaccttt acctctaaca tcgcctacac tattgtattt agatacccat tgatccattc
    10741 gtttcctaac atattcccct ttttccgcac tagcctgaag aagcgactga tcttcaatca
    10801 tttgaattgt ggctaatgca gcttcacaac taactggatt agcacctgtt gtaaataaat
    10861 gtgctggtgc ttctaaacaa ttcataatct ctttgcgtcc aacaattgct gacataggca
    10921 taccacctgc taaggatttt ccgaaagtga ttaaatcagg cgtaaaatta aaatgcgaga
    10981 ctgaactcca tgtacctgtt ctcccaaaac cttgttgaat atcatcgaca gcgattaaaa
    11041 taccatgttc acgacaaatc ttttctaacg cttcaaaata ccctggaact ggttctaaaa
    11101 gtccaccatc gccttgtatc gtttcaataa caatacatgc tacttcgtca gcaggtacat
    11161 acttcgcaaa catttctttt aagggtgcta aatattcttc tactgaatta gcttgtggct
    11221 gctcgtacat accacgatat ttatctggaa acggaatatg ataaaaacca ttcaataacg
    11281 gaccataatg tttgcgcata tttaaactaa tagctgacat agacaatgag ccaaaagttg
    11341 aaccatgata tgcattagtg aaactaatga tataaggacg ccctgtatat gctctggcaa
    11401 atttaatgat gccatcattt gcgtctgatc cggttaatcc gaaggtcact cttttttcaa
    11461 aatctccagg tgcaatctca caaagcttct tagctaaacg taccaacggt tcatgataca
    11521 tatatgctgg tgtataatga ataaatttat caacttgtgc ctttatcgct tctgttactt
    11581 ctctaggtgc atgacctacg ttttgagaac tcgcactcga taacaaatcg atatatgtct
    11641 tcccctcaat atcaaccaat gttgctccat atccatgatc aatcactaac ggataatatt
    11701 taatacgtcc tgatttcgca aaataatgtt catcctcttg aattaattga tgtgctttac
    11761 tcatatacat tcccctccca ttgacagtat taatgtgcaa gcaaagtggc ctctcttaaa
    11821 tcgattataa aatccgattt aaaatcctgc tcaaataccg tcatgcctct atgaatacta
    11881 tccaatatac taataaaatt cagatttgta aatatattct gataattaag aattttatat
    11941 caatctactt tgtttatgcg cataattttg ttcgattagg gaattgttta ctatattcca
    12001 tttgactctg aggtgatacg aatgcgttta aaagtattgt tccatttcat tgctgctatt
    12061 ttcatatcat tcatgttgct atggatgacc atgttgtttg acttaataag caatcaatca
    12121 catttaaaag ctttattatt gaatttagat tttctaattc cttctgacaa tacaccatat
    12181 attctagaaa taatatgtca cttactaatt ggtagcgtca tttattttgt atttgtgttg
    12241 ctgtttcata cttctaaaag gctttactat ctatgttata tccctttatt tttcttattt
    12301 atagcattgt atcccttttt agtatttatt gcgcaacgtc caatttttca atttagtgtg
    12361 acagaattaa tcggttggat tattacacat attttcttta tgtcattaat ggcgttagtc
    12421 atacctagaa taaaataata tttttaatta aactgacaat tatttttcac attttataca
    12481 cttttcaagg tttatgactt gagaaatgtt ctcattttta tcatactgga ggtgtattga
    12541 aaagttaagg agtggaaagt atgaataaag agcaattaga aaaaatgaaa aatggaaaag
    12601 gctttattgc cgcattagac caaagtggtg gtagtacacc aaaagcactt aaagaatatg
    12661 gtgtaaacga agatcaatat agcaatgaag acgaaatgtt ccaacttgtt cacgatatgc
    12721 gtacacgtgt ggtaacttca ccttcattct caccagataa aattttgggt gctattcttt
    12781 tcgaacaaac aatggatcgc gaagtagaaa gcaaatacac tgcagattac ttagctgata
    12841 aaggtgttgt tccgttctta aaagtagaca aaggtcttgc tgaagagcaa aatggtgttc
    12901 aattaatgaa accaatcgac aacttagaca atttattaga ccgtgcaaac gaacgtcaca
    12961 tttttggtac aaaaatgcgt tctaacattt tagaattaaa tgagcaaggt atcaaagacg
    13021 ttgttgaaca acaatttgaa gttgctaaac aaattattgc taaaggttta gttccaatta
    13081 tcgaaccaga agttaatatt aatgcaaaag acaaagctga aattgaaaaa gtattaaaag
    13141 ctgaacttaa aaaaggttta gatagcttaa atgctgatca attagtaatg ttgaaattaa
    13201 caattcctac tgaaccaaac ttatacaaag agttagctga acatcctaat gttgttcgtg
    13261 tcgttgtatt atcaggtggt tacagcagag aaaaagcaaa tgaattactt aaagataacg
    13321 ctgaacttat cgcaagtttc tcacgtgcat tagctagtga tttaagagct gatcaatcta
    13381 aagaagaatt cgataaagct ttaggtgatg ctgtagaatc aatctacgac gcatctgtaa
    13441 acaaaaacta atttatacat taaactattt tttgatataa actactaaaa aggactcata
    13501 catgtgcttt aaaggcattg tgtatgagtc ttttttgatt aaagttgatt ttgccgtatg
    13561 tattagatta cgtaaaaaaa tcggacagct gaaaaatctt taagatttct cagttgtccg
    13621 attttaaata tagctattag aatttagttt tcgtagtaac ctaattctaa gtcttttgaa
    13681 gtttgtttac ggatttttct cattaatttt tcgtcttcaa ttaatgattc accgtatgat
    13741 ggaatcattt tcttaatttt aggtgcccat tcagttttgt attcagggaa gttacgttct
    13801 aatacttcta acgcaactga aactgaagtt gaagcccctg gtgattcacc taataatgca
    13861 attacagtgt ggtcttgtga gttaaccact tctgtaccga attggatgaa tcctttaccg
    13921 tgttcaggtg tatctttgat aacttgtaca cgtttaccag cagtgtataa ttgccaatct
    13981 tcattacgtg cttctggata gaaagtacgt aagtggttca tacaaccttc ttttgtcata
    14041 attacttggt caaatgagta tttaattaaa ggtaagtttt taactgctgc tgctaataaa
    14101 gttgtaatgt tgtatgtttt aacagacttg aataaatcta agttagaacc atttttcaag
    14161 aatttaggtc caacattagc aaatggtcca aataataatg ttctttgacc atcaatgtaa
    14221 cgcgtatcta aatgaggtac agtcattggt ggtgtaccag gtggctcttt accataaact
    14281 ttggcatcgt gttgttcaat aacttgtggg tttgtacaag ctaagaattg accactgata
    14341 gggaatccac ccaaatgttt actttcaggg atacctgttt tttgtaataa tggaattgct
    14401 ccaccgccag caccgatgaa tacgtagtca gttacttgtt tgaatttctc accagttagg
    14461 cgatttttaa cagtaacttc ccattgacca tttgataatt gttcaaaatc aacaacttca
    14521 tggttaaatt gcactgtagc atttggatgt gcttcaatgc ttttagccat tttacgtgtt
    14581 aattcaccga agtttacatc tgtaccttcg tcaattttac ttgccgccat gataccaggg
    14641 ttatcttcac ggcctttcat catcaatgga atccattttt tcattacttc gatgtcttca
    14701 gtatattcga tattatcgaa catagggaaa gctttcatcg cttcgtaacg atcttttaag
    14761 aatttaacat tgtttttacc tctaacataa ctgatgtgtg gtaatggatt gataaattct
    14821 cttgggttct cgatgctacc gcttttcact aagtgacccc agaattgttt tgaaatctca
    14881 aactcttcgt taatcacttt cgctttttcg atgtcgatag aaccatcagg ttgtaaaact
    14941 gtgtagttca actcacataa tgctgcatga cccgtaccag cattatttct ttcgtttgaa
    15001 ctttcgattg caggacgatc caagcgttcg taaacgtgga tattccagtc tggctcaatt
    15061 tcttttaaca ttgaaccaaa tgttgtgcta agtacaccgg ctccaattaa aacgatgtct
    15121 ttactattag acttagccat tggtttcacc tctccaaaaa ttgtaaatgt gtatcatcaa
    15181 tatgaaagtt acataaaact gacatatttc tttaaaatat caacgccatt gataacttcc
    15241 tgttttaatt gatacgctgt aacaaaatac tatagttagt gcttacatgt atatgttaaa
    15301 gcaagcagtg gtaaatgtaa attataatta ttcattaact ttgcaatata ttaaatcttt
    15361 tattcataga agataaatat caaatcaatc ataattattt gacaacaaat agctaacgat
    15421 tgttttaatc tacatttggc ttatagcatt ttaa
//
LOCUS       DAMQYT010000044        15422 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMQYT010000044 DAMQYT010000000
VERSION     DAMQYT010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15422
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.044"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..155
                     /locus_tag="PGA33_001069"
     CDS             <1..155
                     /locus_tag="PGA33_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HDG6472894.1"
                     /translation="EIYIINNTKNLIYHLYDDRGLWLAFNNNEDYARYSEKYNDLISE
                     FDNEDI"
     gene            complement(248..409)
                     /locus_tag="PGA33_001070"
     CDS             complement(248..409)
                     /locus_tag="PGA33_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472895.1"
                     /translation="MLLILELRFWNLEFGFRNSTFGIQNYNEWKILIIACLMTWFCSD
                     FYLMNCFFV"
     gene            complement(446..1186)
                     /locus_tag="PGA33_001071"
     CDS             complement(446..1186)
                     /locus_tag="PGA33_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDG6472896.1"
                     /translation="MFNLKHDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNVCHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLMTE"
     gene            1392..2183
                     /locus_tag="PGA33_001072"
     CDS             1392..2183
                     /locus_tag="PGA33_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDG6472897.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            2551..2979
                     /gene="lacA"
                     /locus_tag="PGA33_001073"
     CDS             2551..2979
                     /gene="lacA"
                     /locus_tag="PGA33_001073"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HDG6472898.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            2995..3510
                     /gene="lacB"
                     /locus_tag="PGA33_001074"
     CDS             2995..3510
                     /gene="lacB"
                     /locus_tag="PGA33_001074"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HDG6472899.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            3523..4455
                     /gene="lacC"
                     /locus_tag="PGA33_001075"
     CDS             3523..4455
                     /gene="lacC"
                     /locus_tag="PGA33_001075"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HDG6472900.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            4459..5439
                     /gene="lacD"
                     /locus_tag="PGA33_001076"
     CDS             4459..5439
                     /gene="lacD"
                     /locus_tag="PGA33_001076"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDG6472901.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            5461..5772
                     /locus_tag="PGA33_001077"
     CDS             5461..5772
                     /locus_tag="PGA33_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDG6472902.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            5778..7490
                     /locus_tag="PGA33_001078"
     CDS             5778..7490
                     /locus_tag="PGA33_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDG6472903.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            7508..8920
                     /gene="lacG"
                     /locus_tag="PGA33_001079"
     CDS             7508..8920
                     /gene="lacG"
                     /locus_tag="PGA33_001079"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDG6472904.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIIDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            9186..10055
                     /locus_tag="PGA33_001080"
     CDS             9186..10055
                     /locus_tag="PGA33_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDG6472905.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIIMVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
     gene            10338..11339
                     /locus_tag="PGA33_001081"
     CDS             10338..11339
                     /locus_tag="PGA33_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDG6472906.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            11573..12574
                     /locus_tag="PGA33_001082"
     CDS             11573..12574
                     /locus_tag="PGA33_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDG6472907.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISTNPAENEGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEIPLVDYIF
                     CTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTP
                     DMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVINI"
     gene            12881..14443
                     /locus_tag="PGA33_001083"
     CDS             12881..14443
                     /locus_tag="PGA33_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDG6472908.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPTGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLIKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
     gene            14618..15154
                     /gene="amaP"
                     /locus_tag="PGA33_001084"
     CDS             14618..15154
                     /gene="amaP"
                     /locus_tag="PGA33_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDG6472909.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            15167..15406
                     /locus_tag="PGA33_001085"
     CDS             15167..15406
                     /locus_tag="PGA33_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDG6472910.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
BASE COUNT         5393 a         2272 c         2940 g         4817 t
ORIGIN      
        1 ctgaaatata tataattaac aatactaaaa acttaatata tcatttatat gatgatagag
       61 gtctatggct agcttttaat aataatgaag attatgcaag atattctgaa aaatataatg
      121 atttaatatc ggaatttgat aatgaagata tctaattttt taatattcgt agcttttatt
      181 tttctactta ttttatgttt atttttaatc ttacaaatga caaaccatta aaagtagcat
      241 cccaacatca aacaaaaaaa caattcatca aataaaaatc gctacaaaac caagtcatta
      301 aacacgcaat aattaaaatt ttccactcat tataattctg aattccaaat gtcgaattcc
      361 gaaaaccaaa ctccaaattc caaaaacgca actccaaaat taaaagcatt tccctaccat
      421 tcgggaaatg ctttttacat actgattact ctgtcattaa tgattttaca acggaaacca
      481 tgtcgtcatg tatgaccaaa gtagcgtcgc tatcataagg tgttcgatct ttattgataa
      541 taattaaatt gtcgccttta aaatgtgata ttaatcctgc ggcaggttgt acaacgagtg
      601 atgaacctag tacaacaagg gtgtcagcat gttcaatttt atttaatgcc cttatgatgg
      661 taggttgatc taacatttca ccgtataata cgatgtccgg tcgaatggca ccaccacaat
      721 tatcacaatg tttcaaagtt ctatcaataa catctgactt cgtataagat ttatgacata
      781 cattacaata aaaacgattt aacgtgccat gtaattcatc aacatgttga cttccagcgt
      841 ctgagtgcaa accatcgata ttttgcgtga tgacacctaa agattgttga ttacgttcta
      901 attttgcaat ccaatcatga acgatattgg gcatcgtatc gacaaatagt aagcgcttat
      961 ggcagaaatt gataaaacct tcaggatcat cttctaaata atcacggctt aacaagtatt
     1021 ctggcgaaag cccatctttt gaaatttcat caaataagcc acccattgaa cggaaatctg
     1081 gaacgccact tgcgacagat acaccagcac ctgtaaaaaa tgtaatacga ttcgaactat
     1141 ctataatatg ttttagtgtc tctaaatcat gtttcaagtt aaacacccca tcaaaataat
     1201 gaatgattta taacgtcttt tacttttatt atagtatatt cattttacga ttatcaaaag
     1261 cgcagtttaa acagttcttt ttaaaaatga attatacata taatcgaaca gttaaattat
     1321 attagttttt acgcaatttt tattatagaa taagcatgta tattaaaatt gatgtctaaa
     1381 cataaaggat aatgacccat cgcctaaata aaggaggcaa ttttacattg aataaacatg
     1441 aacgtttgga cgaaattgct aaactagtga ataaaaaggg cacgataaga acgaatgaaa
     1501 tcgtcgaagg tttaaatgtg tctgatatga cagttcgaag agatttaatt gaattggaaa
     1561 ataaagggat tttaacgaag attcatggtg gtgcacgcag taattcaaca tttcagtata
     1621 aagaaatatc gcataaagaa aaacataccc gtcaaattgc agaaaaacga tttatagcta
     1681 aaaaagctgc atcattaatt gaagatgggg atactttgtt tttcggacca ggaacaacag
     1741 tggaactatt agcagaagaa gtcaatcatc atacgctcac aattattacg aattgtttgc
     1801 cggtgtataa aattttgtta gaaaaacaaa cagcacattt tcgtgtctat ttaattggtg
     1861 gtgaaatgcg ccatattaca gaagcatttg taggtgaaat ggcgaacgcc atgttggaaa
     1921 aactaagatt tagcaagatg ttctttagta gtaacgcagt aaataaaggt gctgtgatga
     1981 catctacatt agatgaagcc tatacgcaac aactggcact aagtaattca attgaaaaat
     2041 acttgttaat cgatcatacg aaagttggca aagaagattt tacatcattt tgtcagttaa
     2101 acgaattgac tgcagtggtc atggactatg aagatgaaga aaaagtagaa acgattaaaa
     2161 catatattga agtagtcgac taaattaaaa gtataactgc attgttaaaa tatgtatcta
     2221 tatgctcata agtgaatgtg aatttgcatt catttatggg ctatttttta tgcaaattta
     2281 tgtcaacggg aacctcgaca aagttttact acaatttcag acgaatacct aattaaaaca
     2341 acaccacaat atgaatgttc acttattgtt tgaaaatacg tcataaacaa acatattata
     2401 aacatattat gttgatttat tgtttgttta tgtttataat ttaaacgtaa tcaaattgaa
     2461 agccttttct caagattttc ataacctaca aataaatatg tcacatttta agaaaggtat
     2521 ttcaaaatta aagtaaaaag gagtcttatt atggcgatta ttattggttc agatgaagct
     2581 ggcaaacgat taaaagaagt catcaaatca tacttattag acaataaata tgatgttgtt
     2641 gacgtaacag aaggacagga agttgacttt gttgatgcaa ctttggctgt agcaaaagat
     2701 gttcaaagtc aagaaggtaa cttaggtatt gttatcgatg catttggtgc aggtagcttt
     2761 atggttgcta caaaaatcaa aggcatgatt gctgctgaag tatcagatga acgctctgga
     2821 tatatgacta gaggccataa caattcaaga atgatcacta tgggatctga aattgttgga
     2881 gacacattag cgaaaaatgt cgttaaaggt tttgttgaag gtaaatacga tggtggtaga
     2941 caccaaatcc gcgtagatat gcttaataaa atgtgctaag gaggaaaaat tactatgaag
     3001 attgcattag gatgcgacca tattgttaca gatacaaaaa tgcgtgtatc tgaattttta
     3061 aaatcaaaag gacatgaagt cattgacgta ggaacatacg atttcacaag aacacattat
     3121 ccaatttttg gtaaaaaagt tggcgaacaa gttgttagcg gtaatgcaga cttaggtgtt
     3181 tgtatttgtg gaacaggtgt tggtattaac aatgctgtaa ataaagtacc tggcgttcgt
     3241 tcagcactag tacgtgatat gacatcagcg ttatacgcta aagaagaatt aaatgcgaac
     3301 gttattggct tcggtggacg tattataggt gagttattaa tgtgcgatat tatcgatgca
     3361 tttattaatg ctgaatataa accaactgaa gagaacaaaa aattaatcgc taaaattaaa
     3421 catttagaaa caagcaatgc agatcaagct gatccacatt tctttgatga attcttagaa
     3481 aaatgggaca gaggcgaata ccacgattaa gggacgacac atatgatttt aactttaaca
     3541 ttaaatccat ccgttgatat ttcataccca ttaacagcat tgaaattaga tgatgttaat
     3601 cgtgttcaag aagtaagtaa aacagctggt ggtaaaggtt taaatgtaac aagagtactt
     3661 gcgcaagtag gtgagccagt ccttgcgagt ggttttattg gcggtgaatt aggtcaattt
     3721 attgctaaaa aattagatca tgccgacatc aagcatgcat tttataacat taagggtgaa
     3781 acacgaaact gcattgcaat tttacatgaa gggcaacaaa cagaaatttt agagcaggga
     3841 ccggagattg ataatcaaga ggctgcagga tttattaaac attttgaaca attattagaa
     3901 aaagttgaag cagttgctat ttcaggtagc ttacctaaag gattaaatca agattattat
     3961 gcacaaatca ttgaacgatg ccaaaacaaa ggtgtgcctg tgatattaga ttgctcgggt
     4021 gcaactttgc aaacagtttt ggaaaatcca tacaaaccta cagtcattaa accaaacatt
     4081 tctgaactat atcaattgct taaccagcca cttgatgaaa gtttagaaag tttgaaacag
     4141 gcagtgtcac aacccttatt cgaagggatt gagtggatta ttgtgtcttt aggtgcacaa
     4201 ggtgcattcg ctaagcacaa tcatacattc tatagagtga atattccgac aataagtgta
     4261 ttaaatccgg ttggttcagg tgattctaca gttgctggaa ttacttcagc aattcttaat
     4321 catgaaaacg accacgattt actaaaaaaa gccaatactt taggcatgct gaatgcacaa
     4381 gaagcacaaa caggttatgt caatttaaat aattatgatg atttattcaa tcaaattgaa
     4441 gttttagagg tgtaaaaaat gtcaaaatcg aatcaaaaaa tcgcatcaat tgaacaatta
     4501 agtaacaacg aaggtatcat ttctgcatta gcatttgacc aacgtggtgc tttaaaacgc
     4561 atgatggcaa aacatcaaac tgaagaacca actgtagctc aaattgaaca attaaaagta
     4621 ttagttgcgg aagaattaac acaatatgca tcttcaattt tattagatcc tgaatatggt
     4681 ttaccagctt cagatgcacg taacaaagat tgtggattat tacttgctta tgaaaaaact
     4741 ggatatgacg taaacgctaa aggtcgttta cctgactgtt tagtagagtg gtctgcaaaa
     4801 cgtttaaaag aacaaggcgc aaatgctgtt aaattcttac tttactatga tgttgatgat
     4861 gctgaagaaa ttaacattca aaagaaagca tacattgaaa gaatcggttc agaatgtgtt
     4921 gcagaagata ttccattctt tttagaagta ttaacatacg atgacaacat tcctgataac
     4981 ggtagtgttg aattcgcaaa agttaagcct agaaaggtta acgaagcaat gaaattattc
     5041 tcagaaccac gctttaacgt tgatgtattg aaagttgaag taccagtaaa catgaaatat
     5101 gtagaaggct ttgctgaagg tgaagtggtt tatacaaaag aggaagcggc acaacacttt
     5161 aaagatcaag atgcagcaac tcatttacca tacatttact taagtgctgg tgtttctgcg
     5221 gaattattcc aagaaacttt aaaatttgct cacgaagctg gtgctaaatt caacggggta
     5281 ctttgtggcc gtgctacatg gtctggtgct gttcaagtat acattgaaca aggtgaagat
     5341 gctgctcgtg aatggttacg tacaactggt ttcaaaaata ttgatgactt aaataaagta
     5401 ttaaaagata ctgcgacatc ttggaaacaa agaaaataag tttaaaaggg gattaatgct
     5461 atgaatagag aagaagtcca attattaggt tttgaaattg ttgcatttgc aggggatgca
     5521 cgttctaagt ttttagaagc attaacagca gctcaagctg gagattttgc aaaagcagat
     5581 gcactgattg aagaaggaaa caattgcatt gcggaagcgc atagagcaca aacaagcctg
     5641 ttggctaaag aagcgcaagg tgatgatatt gcatacagtg taacgatgat gcatggtcaa
     5701 gatcatttaa tgacaacaat tttactgaaa gatttaatga agcatttatt agaattttat
     5761 aaaagagggt gatgacaatg atgcaaaaat tgatcgcaca aattgaaaaa gggaagccat
     5821 ttttcgagaa attatcacgt aacatatatt taagagccat ccgtgatgga tttatttctg
     5881 caatgcctgt catcttattc tcaagtatct ttctattaat tgcatatgtg ccaaatatat
     5941 ttggttttaa atgggataaa ggcatggaag ccattttaat gaaaccatac aactatacga
     6001 tggggcttgt agcattttta gtagcaggaa cgacagcgaa atctttaaca gattcattta
     6061 accgtaagct agaaagtaca aatcaaatta actttatttc tacaatgcta gcagcgatgt
     6121 gtggattctt gttcttagct tctgatccag ccaaagatgg tggattctta agtgcattta
     6181 tgggaactaa aggtttatta acagcattct tatcagcatt tgtaactgtt attgtttata
     6241 acttctgtgt gaagcgcaat attacaatta aaatgccgaa agaagtaccg ccgaatattt
     6301 cacaagtatt taaggactta attccatttt cagcggtaat catcattctt tatgcattag
     6361 atttagtcat tcgcaacagc tttaaatcaa atgtagcgga aggtatttta aaattattcg
     6421 aaccattatt tacagcagca gatggatgga ttggtgtcac aattatcttt ggtgcctttg
     6481 cattattctg gtttgtaggt attcatggtc cgtcaattgt agagccagca attgcagcca
     6541 ttacatatgc gaatatcgaa gcgaacttca agttgcttca agctggagaa cacgcagata
     6601 aaattattac atcaggtaca caaatgttta tcgttacatt tggaggtacg ggtgcaacat
     6661 tggtcgtccc gttcatgttt atgtggatga cgaaatctaa acgaaacaaa gcgattggac
     6721 gtgcatcagt tgtaccaaca ttctttggtg taaacgaacc aattttattt ggtgcaccac
     6781 tagtattgaa tcctgtgttc tttattccat ttgtattagc accaattgtt aacgtatgga
     6841 ttttcaaact tttcgttgaa gtgttaggaa tgaatagttt tagtgtgaat ttaccttgga
     6901 caacaccagg tccattgggc attatcatgg gtacaggttt tggtttatgg tcattcgtac
     6961 tagctattac tttgattgtt gtagatatta ttatttacta cccattccta aaagtttatg
     7021 atagtgaaat tcttgatgaa gaagaaggac gtaaagaaag taattcagat ttaaaagaaa
     7081 aagttgcagc aaactttgat acgaaaaaag ctgattcaat tttagcggca agtggtgtat
     7141 cagacgatgc agctaaagct tcaaatatca cagaacaaac aaatgtctta gtactttgtg
     7201 ccggtggtgg tacaagtgga ttattagcca atgcacttaa taaagcagct gaagaatatc
     7261 atgtgccagt gaaagcggca gctggcggat atggcgcaca tatggacatt atgaaagagt
     7321 accaattaat cattttagcg ccacaagttg cgtctaacta tgaagatatt aagcaagata
     7381 cagatagatt aggtattaaa ttagcgaaga ctcaaggtgc tgaatatatc aaattgacac
     7441 gagatggcca agcagccctt gatttcgttc aacaacaatt tgaaaattaa gaataggagt
     7501 ttttcatatg actaaaacat tacctgaaga ttttattttt ggtggagcaa cagcagcata
     7561 tcaagcagaa ggtgcgacaa atacggatgg taaaggtcgc gtagcatggg atacgtattt
     7621 agaagaaaac tattggtaca ctgcagaacc agcgagtgat ttttataaca gatatccagt
     7681 ggatttagaa ttaagtgaaa aattcggtgt taatggtatc cgaatttcaa ttgcatggtc
     7741 tcgtatcttc ccaaatggat atggcgaagt taaccctaaa ggtgttgagt attaccataa
     7801 gttatttgca gagtgtcata aacgtcatgt tgaaccattt gtaacattgc atcattttga
     7861 tacaccagaa gtgttacaca aagatggcga ttttctaaat cgtaaaacga ttgactactt
     7921 tgtagattat gctgaatatt gttttaaaga attcccagaa gtaaaatatt ggactacatt
     7981 caatgaaatt ggacctattg gtgatggtca atatttagta ggtaaattcc ctccaggtat
     8041 taaatatgat tttgaaaaag tattccaatc acatcataac atgatggtgg ctcatgctag
     8101 agcagtaaaa ttatttaaag atggcggata caaaggagaa attggtgttg tacacgcatt
     8161 gccaacaaaa tatccgttcg atccatcaaa tcctgaagat gttcgtgcag cagaattaga
     8221 agatatcatc cataataaat tcatcttaga tgcaacatat ttaggtaagt attctcgtga
     8281 aacgatggaa ggtgtgcaac atatcttatc tgttaatggc ggtaaattaa acattacaga
     8341 tgaagattat gccattttag atgcagccaa agatttaaac gacttcttag gtatcaatta
     8401 ctacatgagt gattggatga gaggttacga tggagaatct gaaatcacgc ataatgcgac
     8461 aggtgataaa ggcggttcta aataccaact taaaggtgtc ggacaacgtg aatttgatgt
     8521 cgacgtacct cgcacagact gggactggat gatctatcct caaggtttat atgatcaaat
     8581 catgcgtgtt gttaaagatt atcctaacta tcataagatt tatatcactg aaaatggttt
     8641 aggatataaa gatgaattta ttgaatctga aaaaacagtt catgatgatg cacgtattga
     8701 ttatgtaaga caacatttga acgttattgc agatgctatt atagatggtg caaatgttaa
     8761 aggttacttt atttggtcat taatggatgt gttctcttgg tcaaatggtt atgaaaaacg
     8821 atatggttta ttctatgtcg attttgagac tcaagaacgt tatcctaaga aaagtgctta
     8881 ttggtacaaa gagttagccg aaacaaaaga aattaaataa ttatagatat taaaaggcct
     8941 atcagtcgag tgctggtagg tctttattta tagaaagaga taaattaaac atgcatatac
     9001 atatttatat attaatcaat gggtgacaca ccattgaaag ctaaagaagc aatcaattaa
     9061 caaacgttaa cggacaactt tccaaagtca tcttaaaagt taatgatgta ttcaaaatca
     9121 actaaaggac atttttaatc acatccttta tacttatata aaagacatga atgggagtta
     9181 aaagtgtgaa gaaaattttt gtaattatta cgacattgtt agcggtcgct attattatag
     9241 gctctattat tatggtggtg tttagtcaac gacaggcaca aacatttaaa atacaacaac
     9301 agcaatttgt taaaaagcca attccaacat tgttcttaca tggatttggt ggtagtgcca
     9361 attctgaaaa gtttatggtg aagcaagcag aaaaaagagg ggtaactaaa gatatcatca
     9421 ctgcttacgt ttcaaaggat ggtgcagtga catttaaagg gaaactaaga aaagatgcag
     9481 tgaatcctat tgtgaaaatt gaacttgaaa ataacagaca aggctatttg gataagaatg
     9541 ctgcgtggtt taaaaatgta ctgactaagt tacaatctga atacaatttc gataaattca
     9601 actttgtcgg tcactcaatg gggaacttaa catttgcaca atatatgatg acatatggta
     9661 acgacaagtc actgccacag ttaaacaaac aagtgaatat tgcagggaca tttaatggtg
     9721 ttttaaatat gaatgaagat gtcaatgaaa ttacagttga taaagatggt aaaccaagtc
     9781 gaatgaatca accttatcag caattacgcg tgctaaaaga tatttataaa ggtaaaggta
     9841 tcgaagtgct aaatatttat ggggatttga aagatggtac acattcagac ggcagagtgt
     9901 ctaatagttc ttcgaaatca ttaaaatatt tactgggaaa tagtccgaaa agttatcgag
     9961 aatctaaata tgaaggtgag cctgcacaac atagtcagct tcatgagaat gaaaatgttg
    10021 ctaatgagtt aatcgacttc ttatggaaaa aataaagcaa ctgctagttt ttctggggca
    10081 aaaaagcttg taatattaga gcttttcaaa aattatctat tgtttgagag tttccataaa
    10141 atgtctaagt cgtaacattt taaatttatg attatagcaa atataatgtg gttgaagtta
    10201 catgaatgaa tcagtgctta agtcaaaata taacaactaa gaatgtattt gcatacagac
    10261 atcacgaaga cacaattcaa acttaacgct taagtaacaa ataagtaaaa gtttcaaata
    10321 aagaaaggtg attaatgatg caaaataaac aaattctttt taacaaaata ccagaaggca
    10381 tgcctcaaga ggatacattc aaaattgaag agattgacac gcctaaactt gagagcgatg
    10441 gtgtacttgt acaaacgctg tatatttcag tggatccata tatgagagga cgtatgacta
    10501 aggcagattc gtatgtacag ccttttgaaa ttggtaaacc gattgttagc catgtcgttg
    10561 caaaagtaat tgattcgaca ctcgcagatt ataaaaaagg agatgtcgtt gtaggtatgt
    10621 tgccttggcg aatcattaat catgtccaag cggatcaaat tactaaagtt ccaacgacag
    10681 atgtaccact tgatttatat ttaagtgttt taggaatgcc aggacaaaca gcatatcatg
    10741 gtctgcttga tattggacaa cctaaagccg gtgatactgt tgtggtttca gctgcatctg
    10801 gtgctgtagg ttcagttgta ggtcaaattg ctaagattaa aggttgccgt gtagtaggta
    10861 ttgcgggtgg cgacaaaaaa gtaaattatt taacagagac actgggcttt gacgctggta
    10921 ttgattataa gaaagaggat tttgcagaag cgttagcaca agcagtacca gatggtattg
    10981 atgtttattt tgaaaatgtt ggtggaacag ttggcgatga agtatttaag catttaaatc
    11041 gttttgcacg tgtaccagta tgtggcgcaa tttcgtcata taatcatcct gaagccgata
    11101 tcggaccacg tattcaaggt acgcttatta agaagcaagt aatgatgaga ggcttcttgg
    11161 taagtgaatt cgctaatgct tttaaagaag ctagtgaaca gcttgcaaca tgggttcaag
    11221 aaggtaaaat tcagtcacaa gtaaccattg aagatggttt tgaaaatgcg ccacatgcat
    11281 ttaaaaattt atttactggc gataactttg gtaaacaagt tattaaagta acggaataat
    11341 aaagtatacg ttttaacaaa ctaactttgt taattgacca aaatttaata attttttatc
    11401 taaagtaaat ttgttcagca acatggtaga aataagtcta aaatttaata cgaaagtcgt
    11461 ctgtaattat ttatatattc aaatgaaaaa ggagtcatga tgctgcgata taagcacaca
    11521 tggcttctat tttgtataat aagaaataag ttcataaatg aggtgaaacg atatgaaaat
    11581 gataggattt gagaagccct ttaaattgga agaaggaaat ttatttaaag tatacgaaca
    11641 aagaaagcca acgccagaaa acgatgatat cttagtcaaa gtaaatagca ttagtgtaaa
    11701 tcctgtagat acaaagcaac gacaaatgga agtcactcaa gcacctcgtg tacttgggtt
    11761 tgatgctatt ggtactgttg aagctatcgg accagacgtt acattattta gtccgggtga
    11821 cgttgtattt tatgctggat cgccaaatag acaaggatca aatgcgacat atcaattggt
    11881 gtcagaagct atagttgcaa aagcaccaca caacataagt gctaatgaag cggttagtct
    11941 accgctaact ggtattacgg catatgaaac gttttttgat acatttaaaa tttctacgaa
    12001 tcctgctgaa aacgaaggga agtctgtctt aatcattaat ggtgccggtg gtgtaggcag
    12061 tatagccact caaattgcta aacggtatgg tttaactgtc attacaacag catcacgaca
    12121 agaaacaaca gagtggtgtg aaaagatggg cgcagatatc gtattgaatc ataaagagga
    12181 tttagtgcgt caatttaaag aaattccatt agtagattat atattttgca cctataatac
    12241 agacttgtat tacaatacta tgattgaact gataaaacca ttagggcata ttacgactat
    12301 cgtagctttt aatgaagatc aagatttaaa tgcattaaaa ttaaaaagta taacatttac
    12361 acatgaattt atgtttgcgc gtccaattca tcgaacgcct gatatgatta aacaacatga
    12421 gtatttagaa gatattacga aaaatataga atcagggcat taccaaccaa caacgacaca
    12481 agtttttgaa ggtttatcac ctgaaaactt atatcaagca catcaattgt tggaaaaaca
    12541 atcaatgatt ggaaaattag taattaatat atagattagt aagtaaagtc gccttatgca
    12601 gaaaatactg tataggcgac ttattttttg tgtctagcga ctattgattc taaacggaaa
    12661 tatggcaact ttgcaggtat caaatcaata atgcactaga aaggcattac ttaaatacaa
    12721 taaaatactt tgttgctcaa tgataaataa tttgagtgtg ttttatatat catttttaaa
    12781 agatgaggca ttattaattg tgtctatata aaattacaaa tgattaaaat gttgcagtta
    12841 tgggtatgaa cttaccatta cacagaaagg gtttgtattt atgaaaaata aatcagtaag
    12901 tctagtgttt tgggtatctt tagtgatatg tacgatattt gttgcttttg gagcaatatt
    12961 tccgaaacag ttagaaaaac tcacgcaaaa cattactaca tttattgcgc tacatttttc
    13021 ttggtattat ttattgctcg ttttagttat tttatttgtc tgtgtgtata ttttattttc
    13081 gagatacgca aacattacat taggtgaaga aggcgaagat cctgaattct cattgccgtc
    13141 ttggtttgct atgttattta gtgctggtat gggaatagga cttgtgttct ggacaacggc
    13201 agaaccaatc agtcatgcct tcaaattaac gcctattcat aaagctggca cgcaatctgc
    13261 aattaatgat gcaatgcagt tttcattttt ccattggggg attcatgcat gggcagtcta
    13321 tggtattgtc gcgctagtgt ttgcatattt tagctttcat aaaggatatc ctggcttagt
    13381 cagcgctaca ttgacgcctt tattaggtga aaaggcaatg cgtggacctt taggtggtgc
    13441 cattgatgta ttggcagtca tagctacagt aacaggcgtt gctgcaacat taggtttcgg
    13501 tgcattgcaa ataaacgaag gtttacattt tttatttaat gtaccgagta acttcacgat
    13561 gcaagtaatc ttgattgtca ttgcaaccat attatttact tggtcagcat ggtcaggtat
    13621 cgataaaggt atcaaaacgt tgagtaatat caacatgtta cttgcttttg tagtacttat
    13681 tggtcttttt attgttgggc caacgctata cattttaaat acatttacga atggtctagg
    13741 taattatatt gcaaatttct ttagtatgag tttacgcatt ccgacaggtg gtcaaaaatt
    13801 ccaatggtta caaaattgga caatctttta ctgggcatgg tggatatctt gggcaccatt
    13861 tgtaggtata tttattgcac gtgtgtcaaa aggacgtacg attaaagaat ttattctagg
    13921 tgtacttttt gtcccggcgt tagtttgctt tatattcttt gcggtattcg gcgcgtcagc
    13981 aatttactta caagataatc atattgccga tattgctaaa gctgcgacag aaacagcaac
    14041 ttttgcgacg ctacaacatt atccgctagg ttttgtatta agcttaatta ctttacttgt
    14101 tattatgatt ttctttgtta catcagcgga ttcggcaact tatgttttag gtatgttgag
    14161 ttcaagtggt gatattaatc cgaagtcatt tgtcaaagtg agttggggta tcattatggc
    14221 attatttgca atcatcatga tttacacagg tggtactcaa gcaattcaaa atttattaat
    14281 cattgctgcg ttaccatttt ccgtggtcat tatagcgatg atatggtcgc ttataaaatc
    14341 tttgagtgag gagaaaccga gaaatagtaa taaggtttta attaaacatc gtgacccaga
    14401 tattttagaa tatcgttctt caaaacatat tgacgaaaat taattaataa tgaggtttta
    14461 tttacaaaaa ctagggaata tattactaaa tggtacttca tcttatatat taaaaataaa
    14521 tttaaagtta ttaatttatt atgtttaaga aaaaaactga tgggtacata tttaatatgt
    14581 ttttaaatta actttaacta aggaggtgtc gttaggggtg aaacgactta aaaactttat
    14641 ccttggctta ttaatagtag taatcgtggg gttcctgtta ttcatgtata tccaagatgg
    14701 acgtataact gaatatcaag actacttcct tcaatttgaa tggttccaac cattacttat
    14761 ttcacttgct gcactattga tattaatagg tcttatatta gtatttagta tcttcaaacc
    14821 tacgcatcga aaacctggat tatacaaaga cttcgatgat ggtcatgttt acgtatctcg
    14881 taaagctgtt gaaaagacgg cattcgatac ggttgcaaaa tatgatcaag taagacaacc
    14941 aaatgtagtt gcgaaactat ataacaaaaa aaataaatct tatatcgaca ttaaaactga
    15001 ctttttcgta ccaaataatg ttcaagttca atctttaact gaagcaattc gttcggatat
    15061 taaaaagaat gtagaatatt tcacagaaat gccagtacgt aagttagaag ttaatgtgag
    15121 agatcagaaa acttctggtc cacgagtgtt gtaagggagg tatatcatgg ctaacaatca
    15181 taaccaaaac ggacaagact ctacacaaca agtgattaat tttttaaaag tatttaaatg
    15241 gagaatcgtt ggtttcctag cgtttctatt aatcgcgata ttattcttaa cgttaggttt
    15301 ttggaaaaca gtacttatca tcgttttatg tttaattggt gtaggtattg ggtatatgaa
    15361 agaccgtaag caagatttta tgaatttttt aaatcgatgg agttaattca tcaatttaga
    15421 at
//
LOCUS       DAMQYT010000045        15362 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMQYT010000045 DAMQYT010000000
VERSION     DAMQYT010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15362
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.045"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            128..2650
                     /gene="mprF"
                     /locus_tag="PGA33_001086"
     CDS             128..2650
                     /gene="mprF"
                     /locus_tag="PGA33_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDG6472911.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVAITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAW
                     LFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKAS
                     SLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGEFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(2853..3362)
                     /gene="msrA"
                     /locus_tag="PGA33_001087"
     CDS             complement(2853..3362)
                     /gene="msrA"
                     /locus_tag="PGA33_001087"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDG6472912.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            3499..4482
                     /locus_tag="PGA33_001088"
     CDS             3499..4482
                     /locus_tag="PGA33_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDG6472913.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            complement(4570..4755)
                     /locus_tag="PGA33_001089"
     CDS             complement(4570..4755)
                     /locus_tag="PGA33_001089"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDG6472914.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            4903..6165
                     /locus_tag="PGA33_001090"
     CDS             4903..6165
                     /locus_tag="PGA33_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDG6472915.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            complement(6302..7393)
                     /locus_tag="PGA33_001091"
     CDS             complement(6302..7393)
                     /locus_tag="PGA33_001091"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDG6472916.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            7558..8589
                     /locus_tag="PGA33_001092"
     CDS             7558..8589
                     /locus_tag="PGA33_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDG6472917.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGN
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            9081..10487
                     /locus_tag="PGA33_001093"
     CDS             9081..10487
                     /locus_tag="PGA33_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDG6472918.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVIKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITELMVIEKYEHVMHIVSEVTGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            10484..11050
                     /locus_tag="PGA33_001094"
     CDS             10484..11050
                     /locus_tag="PGA33_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDG6472919.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTKDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            11056..12054
                     /gene="trpD"
                     /locus_tag="PGA33_001095"
     CDS             11056..12054
                     /gene="trpD"
                     /locus_tag="PGA33_001095"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HDG6472920.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALEKYHQMRGE"
     gene            12056..12838
                     /gene="trpC"
                     /locus_tag="PGA33_001096"
     CDS             12056..12838
                     /gene="trpC"
                     /locus_tag="PGA33_001096"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HDG6472921.1"
                     /translation="MTILSEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYDQYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKTNHYYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            12838..13470
                     /locus_tag="PGA33_001097"
     CDS             12838..13470
                     /locus_tag="PGA33_001097"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HDG6472922.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            13463..14677
                     /gene="trpB"
                     /locus_tag="PGA33_001098"
     CDS             13463..14677
                     /gene="trpB"
                     /locus_tag="PGA33_001098"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDG6472923.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGDDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMVERGLAND"
     gene            14670..>15362
                     /locus_tag="PGA33_001099"
     CDS             14670..>15362
                     /locus_tag="PGA33_001099"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDG6472924.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIK"
BASE COUNT         5379 a         2235 c         2820 g         4928 t
ORIGIN      
        1 acttcttaat acagatatca atatgacaaa agtgttatat aaatcaaagg taaatgatat
       61 gctattttta tggtttatat gatatatttt gatttataac agaaataatt agaattgatg
      121 tgaaaaaatg aatcaggaag ttaaaaacaa aatattttca atcttaaaaa ttacgtttgc
      181 tacagcttta tttatttttg tagcaatcac attgtatcgg gagttatctg gtattaactt
      241 taaagatacg ttggttgaat ttagtaagat taaccgtatg tccttagtgt tactatttat
      301 tggtggtggg gcatcgcttg ttattctatc aatgtatgat gtgattttat ctagagcttt
      361 aaaaatggat atatccttag gcaaagtttt aagagtaagt tatatcatca atgcattgaa
      421 tgcgattgta ggtttcggtg gctttattgg tgcaggcgtt agagcaatgg tttataaaaa
      481 ctatacgcat gataaaaaga aattagttca ctttatatcc ttaatactta tttcaatgtt
      541 gacaggttta agcttattat cattgctaat tgtattccat gttttcgatg catctttaat
      601 cttagataag attacatggg taagatgggt attatatgta gtgtcatttt tcttaccatt
      661 attcattatt tattcaatgg ttagaccacc cgataaaaac aatcgttttg taggattgta
      721 ctgcacttta gtgtcgtgtg ttgaatggtt agcagctgca gttgtattat atttctgtgg
      781 tgtaattgtt gacgctcatg tatcattcat gtcctttatt gcaatattta tcattgctgc
      841 attatcaggt ttagtcagct ttattcctgg tggtttcggc gctttcgatt tagttgtatt
      901 actaggattt aaaactttag gtgtccctga ggaaaaagta ttattaatgc tacttctata
      961 tcgttttgcg tactattttg taccggtaat tattgcatta attttatcat catttgaatt
     1021 tggtacatca gctaagaagt acattgaggg atctaaatac tttattcctg ctaaagatgt
     1081 tacgtcattt ttaatgtctt atcaaaagga tattattgct aaaattccat cattatcatt
     1141 agcaatttta gtattcttta caagtatgat cttttttgta aataacttaa cgattgttta
     1201 cgatgcttta tatgatggaa atcacttaac gtattatatt ctattggcaa ttcatactag
     1261 tgcttgttta ttacttttac tgaatgtagt tggtatttat aagcaaagta gacgtgccat
     1321 tatctttgct atgatttcaa ttttattaat cacagtggcg acattcttca cttacgcttc
     1381 atatatttta ataacatggt tagctattat ttttgttctg cttattgtag ctttccgtag
     1441 agcacgtagg ttgaaacgcc cagtaagaat gagaaatata gttgcaatgc ttttattcag
     1501 tttatttatt ttatatgtta accatatatt tattgctgga acgttatatg cattagatat
     1561 ttatacgatt gaaatgcata catctgtatt gcgctattac ttctggctta cgattttaat
     1621 catcgctatc atcataggta tgattgcatg gttgtttgat tatcaattta gcaaagtacg
     1681 tatttcttct aaaattgaag attgcgagga gattattaat cagtacggcg gtaattattt
     1741 gagtcacttg atatatagtg gtgacaagca gtttttcact aatgaaaata aaacagcatt
     1801 tttaatgtat cgttataaag caagttcatt agtggttctt ggagatccgt taggtgatga
     1861 aaatgccttt gatgaattgt tagaagcatt ctataattac gctgagtatt taggctatga
     1921 tgttatattc tatcaagtta cagatcaaca catgccttta tatcataatt tcggtaacca
     1981 atttttcaaa ttaggtgaag aagcaattat tgatttaacg caattttcaa cttcaggtaa
     2041 aaaacgccgt ggatttagag cgactttaaa taaattcgat gaacttaata tttcgttcga
     2101 aattattgaa ccaccgtttt caactgaatt tataaatgaa cttcaacatg taagtgattt
     2161 atggctagat aatcgtcagg aaatgcattt ctctgttggt gaatttaatg aagaatactt
     2221 atctaaagcg ccaattggtg taatgcgaaa tgaagaaaat gaagtaattg cattttgtag
     2281 tttaatgcca acatacttta atgatgccat ttcagtcgat ttaattagat ggttgccaga
     2341 gttagattta ccattaatgg atggtctata cttgcatatg ttactttgga gtaaagaaca
     2401 aggttataca aaatttaata tgggtatggc aacgttatcg aacgttggtc aattgcatta
     2461 ttcatattta agagaacgac ttgcaggccg tgtctttgaa catttcaacg gtctatatcg
     2521 tttccaagga ttacgtcgtt ataaatctaa atataatccg aattgggaac cacgcttttt
     2581 agtttatcgt aaagataatt cgctttggga atcactttct aaagtaatgc gtgtaatacg
     2641 tcacaaataa ttaaaatcca agtgctaaga ggtatacagt tatgcctgat gcgcttggat
     2701 tttttggttt ctataaagaa tgatttataa aaaacggtac agataagaat atttaagttt
     2761 cctatctgta ccgtatttta atattgaaat gagctaaagt taagtcgcct aacttttatt
     2821 atcagtccaa tcagtttcat caagtataaa tgttattgct tatttttgta ttcttggcgt
     2881 attttttgtt cttctgcata gcgctctgga tttttcttat aaaaatcttg gtgatagtct
     2941 tcggctttgt aaaattgtga cgctggtaat atttttgttg caattgcctt atcagcatta
     3001 atcgtatttt taagctgctc gatataagtc tcagcgagtt ctttttgatg atcattagtg
     3061 tagaaaatag ctgtttgata ttgaggacca cggtcttgat attgaccacc tgtatctaat
     3121 gggtcaatga ctgagaaaaa tatttctaat aacttattgt atgagaataa tgcaacatca
     3181 tattgaattt caacagtttc taaatgacca ctcgtacctg attttacttg ttcgtaagta
     3241 ggattttcaa tatgtccgcc catatatcca gaagttactt tttctatgcc gtcaaaggtg
     3301 tcaaatggtt tcgtcataca ccaaaagcaa cctccggcaa aataagctgt attaatattc
     3361 atttttgaca tcctttcatt agaccttagt acgatttatt aagaaatcac ttgctttttg
     3421 aattgttttt atataacgtt aatatgtgat tattatagta cacaaaaatc tatttttgaa
     3481 gatataaggt aggtaaatat ggataaagaa actaatgaca acgaatatag acgtcaaagt
     3541 gaacatcgca cttcggcgcc taagcgaaaa aagaagaaga aaattaggaa attacctatc
     3601 attcttctga ttgttgtaat tttacttatc gcattagttg tatatattgt acatagttac
     3661 aatagcggtg tagaatatgc caagaaacat gcgaaagatg ttaaagtaca tcaatttaat
     3721 ggaccagtaa aaaatgatgg taaaatttct attcttgtac tcggtgcaga taaagcacaa
     3781 ggtggacaat caagaacaga ttctatcatg gttgttcaat atgactttat caataaaaag
     3841 atgaaaatga tgtctgtcat gcgtgatatt tatgcagata ttccaggata tggaaaacac
     3901 aaaattaatt cagcatacgc tttaggtggt ccagagctac ttagaaaaac acttgataaa
     3961 aatttaggaa ttaatcctga atattatgca gtagttgatt ttactggatt tgagaaaatg
     4021 attgatgaat taatgcctga aggtgtacca attaatgtcg aaaaagatat gtcgaaaaat
     4081 attggtgtat ctttgaaaaa gggtaaccat aggttgaatg gtaaagaatt acttggttat
     4141 gcaagattcc gtcacgaccc tgaaggtgac ttcggacgcg tgcgacgtca gcaacaagtg
     4201 atgcaaacat tgaaaaaaga aatggttaat tttagaacag ttgttaaatt accaaaagtt
     4261 gcaggtattt taagaggcta tgtgaataca aacattcctg attcagggat tttccaaaca
     4321 ggtttgagtt ttggtatccg aggtgaaaaa gatgttaagt cattgactgt gccaatcaag
     4381 aactcatacg aagatgtcaa tacaaatact gatggtagtg cattacagat taataaaaac
     4441 acaaataaac aagctattaa agacttttta gatgaagatt aaaaataaac aaggcgattt
     4501 ctatcatact cagatagaag tcgccttgtt cgttttcaaa gaattaattg gcatgcatct
     4561 tatgaagaat tattgatctg actttcttac gccagccaca ccataatggt ttggtttcat
     4621 ttcttctata acaacgtgaa ttgcttgtct atttgccccc gttgtttttt ctacggcgtc
     4681 agttacttcg ctaactaaat tttttaattg ttcatccgaa cgaccttcta ataattttac
     4741 attgacgatt ggcatcatcc aaacctccta atcattatta atttgagtat atcatgtaat
     4801 acttaaaaat tcatttgcaa aataacagaa catttgttct aaaatagttg aaatagaaca
     4861 cgtgttcgta tataataaga ttcaagtaag ggaggaataa cagtgtataa ttatcattta
     4921 ttagaagata gggatgttct atgtattgac caaaaaagtt tttttgcgag tgtttcttgt
     4981 attgaaaagg ggctagatcc attagaaaca aagctagctg ttgttgcaga tactaagcgt
     5041 cagggttctg taatattggc tgcgacacct aaattaaaag aattaggcat caagacaggg
     5101 tcgcgattgt ttgaaatacc acatagaaat gatatttaca ttatcaatcc aagtatgcgt
     5161 aaatatctta atgtttcagt tgctatttct aagattgcat tgcgttatat tccacctgaa
     5221 gatttacacc aatatagtat tgacgaattt tttatggatg ttactgatag ctatcataga
     5281 tttagttcta cagtacatgc attttgcgaa agacttaaac gtgaaattta tgaagaaaca
     5341 ggcatttatt gtactgtggg cattggttct aatatgttat taagtaaaat tgctatggat
     5401 gttgaagcga agcatagtca aaatggtata gctgaatggc gatatcaaga tgtaccaacg
     5461 aaattatggc caattcagcc cttgcgagat ttttggggta ttaatcgtcg aacagaagcc
     5521 aaattgaata aaagaggaat ttttactata ggagatttag cgaaatatcc atataaattt
     5581 ttaaaaaaag agttcggtat tttaggtgtt gatatgcatc tacatgcgaa tgggatagat
     5641 cagagtaaag tacgtgaaaa gcacaagatc agcaatccat cgatatgcaa aagtcaaata
     5701 ttaatgagag attatcattt tgatgaagca aaagtagtaa tgcaagagtt aattgaagat
     5761 gttgctagca gagttcgagc aagaaaaaaa gtggcaagaa cgatacattt tgcctttggc
     5821 tatagtgatg aaggcggtgt acataagcaa tatactttga aagatccaac aaacttagaa
     5881 aaagatattt ataaagtagt aatgcatttc gcagataaat tatgtaataa acaagcacta
     5941 tatcgtacgc taagtatatc tttgagtcaa tttattaatg aggatgagcg acagttaagt
     6001 ctgtttgaag atgaatacca acgcaaacgt gacgaatgtc tagctaaaac gatagaccaa
     6061 ttacatttga aatacggcaa aggtattgtg tccaaagcag tatcgtttac agaagcaggt
     6121 acaaaacacg gcagattagg tttaatggct ggacataaaa tgtaatgact atacggttta
     6181 agtaatatat aactgtgatt cgtataaaat aagtctctaa agataaatat ttcatatatc
     6241 acaatagatt ttcacaataa tatctaagaa tacatggaat ttatcaaaag agacttaata
     6301 attattggat ataacaatca aaatcactca atgcttgcat accgcgttct cggtcagtag
     6361 ggtttttgaa actaattttt aaagcaccgt atatatcttc gcgtacttct aagattctta
     6421 agttgcttat agatatgtta tgtaaactca ggatataagt cactttactt atcatacctg
     6481 attcatccgg aatgtctaca tatagatcat acgcagtatt tagtccacct agttgtttag
     6541 cgggtagtgc gtcgcgatac gatttagctt gggcaaaaaa tgataacaat ttttcagaat
     6601 cattgctttc aattagtctt tctaaatctt gaaactgact ttttagctgt cgaatcattt
     6661 ctaaaatata cgttttatta ctcaaggtga tatctttcca catttgtgca ttactactag
     6721 ctatacgagt gatatcacga aaaccaccag ctgcaagttt attaactaaa tgatgttctt
     6781 gaccgttctt ttgactaaca tgaactaaac tagatgcaac gatatgaggt aaatgactta
     6841 cgacgcttgt tacgtagtcg tgttcttcag cagtagttac aataaattta gcaagagtag
     6901 gtgataacag ttcttttaac gtgtttgctg cttgctcatt tcttggctca ttgtagacta
     6961 aaatataata agcgttttca aataagtgct ttttagcatt tagtacacca gatttatgac
     7021 taccagccat tggatgacca ctgactaaat gaatattatg ctttaataaa ttgcattcgt
     7081 gttgctgtat cattgcttta gtactaccag tatcagaaac aataacacca ggtttagttg
     7141 gcatatctat aagctcgcta agatatttat ttgtgatagc aacaggtgtt gcataaataa
     7201 ttacatcggc ttttttaata gcttcactat aatttaaaca tttttcatta ataatgccga
     7261 ttgatttagc tttatctaac tgagaagtat ctgcatcgta tgcaataata ttagtattag
     7321 ggttatggta ttttatattg ctagcaagac ttccaccaat taatccaagc ccaacaaata
     7381 aaactgttgt catataaatc accttatttc gaaattttca gaataataac attgtaaatg
     7441 agctgttgac acagtgcaat agtaaataaa aatcgataat agcattaata gataaacgga
     7501 gataaatcat ctacaataaa gagtatagta acacaatggc aacggagggg taaatcaatg
     7561 gaaccaatat tagaaatgat taaaacatta acaggtatta atagtccttc aggagacaca
     7621 gaagaagcaa ttcaatttgt cgaaaaatat gcaaaagact tgggttatca aacaacacta
     7681 acaaataaag gtgcgttatt aataacagtg ccaggcaaaa atgatgaagt acaacgctgt
     7741 attactgctc atgttgatac tttaggtgca atggttaaag aaattaaaga agatggtcgc
     7801 ttagcaatag aattaattgg aggattcacg tataacgcga ttgagggtga atattgccaa
     7861 attaaaactg atgctggtca aatatataca ggaacaattt gtctgcatga aacaagtgtt
     7921 catgtatata gaaataatca tgaaatacct agagatcaaa agcatatgga aataagaatt
     7981 gatgaagtaa ctacatcaga agaagataca aagagtttag gtatttcagt aggtaatttt
     8041 gttagctttg atccacgtac agttatcacg tcatcaggtt ttattaaatc tcgtcattta
     8101 gatgataaag ctagcgtagc gatgatacta caattactaa agaaattaaa agaagagcaa
     8161 ataatattac cacatacaac gcaattttat atttctaata acgaagaaat aggttacggt
     8221 gcaaatgcat caattgattc gaaaatcaaa gaatatattg cattagatat gggcgcgttg
     8281 ggagacggtc aagcatcgga tgaatataca gtttctattt gtgccaaaga tgcttcaggt
     8341 ccatatcata agcaattgaa atcgcaccta gttaatcttt gcaaaataaa taacattcca
     8401 tataaagtag acatatatcc atattatggt tcagatgctt cagcagcttt acatgctggt
     8461 gcggatatca gacatggttt atttggcgct ggcattgaat catctcatgc aatggaacga
     8521 acacatattg attctattaa agcgacagag aaattactat atgcatattg cttatcacca
     8581 attgagtaaa caattagtgt tgacaaatgt gaacgaccta tgtaatataa tgaactataa
     8641 aaataattag aattttctaa agaaatagta gcagatatga aacgtagcaa atagaaagct
     8701 aatgggtgat gggaattagc acgccatatc ttgtgaattg gactttggaa aacaattgaa
     8761 tgagttttga aagtgaacat gaattatgtt aactaaggtg gcaccacggt aacgcgtcct
     8821 tacaggtata tgcgttatgt ggtgtctttt tatttagaca aaatgtagta gttaattaaa
     8881 ggtagcaaca gaaagttagt ggatgatgtg aactaacacc gagattaatg aaattgggtt
     8941 ttgtctgcaa cagaaaaatt atatatagta aagagtgaac tatgaatatt tcgaatattc
     9001 ggttaattta ggtggtacca cgcgtcagcg tcctttatat tgataaggat gctggcgctt
     9061 ttttgaaagg agcgtataga atggatatat tttataaaaa aataaaagca aatgtaacgc
     9121 ccgaagtttt agcacaactt cattccaaga agatcatttt ggaaagtaca aatcaacaac
     9181 aaactaaagg tcgctattca gttgttattt ttgatattta tggcacttta actttagata
     9241 atgatgtatt atcagtaagt actttaaaag aatcgtatca aatcactgaa agaccgtacc
     9301 attatttaac gactaaaata aatgaagact accataatat tcaagatgag caacttaagt
     9361 cattaccatt tatatctgga tatgttggga cgtgtagctt tgatttagta agacatgaat
     9421 ttcctaaatt gcaatcaata caattagaag atcacaagca gcacgatgta aggttatata
     9481 tggttgaaca agtttatgta tttgaccatt acaaagatga gttatatatc atcgcgacga
     9541 atcaattttc aaattcaaca aaatcagatc ttgagaatcg agttattaag tctatcgaag
     9601 acttaactaa aatccaacca ttcatgccta cacaagattt tgattttaaa actaaagaaa
     9661 ttcaatcaaa catttctgaa gaaagattta tcgaaatgat tcagtatttc aaagagaaaa
     9721 taacagaagg ggatatgttc caagttgtgc catcaagaat ttacaaatat gcgcatcatg
     9781 ctagtcagca tttaaatcaa ctttcgtttc aactgtatca aaatttaaaa cgacaaaacc
     9841 caagtccata tatgtattat cttaatatcg atcaaccata tattgtcggt agttctcccg
     9901 aaagtttcgt aagtgtcaaa gatcaaattg taacaactaa tcctattgca ggtacgattc
     9961 aacgtggtga gacgacacaa atagataatg agaatatgaa acaactactt aatgatccaa
    10021 aagaatgcag cgaacatcgt atgctagttg atttaggacg taatgatatt catagagtaa
    10081 gtaaaatcgg tacctcaaaa attactgaat taatggttat tgaaaaatat gaacatgtta
    10141 tgcatatcgt aagtgaagtc acaggtaaaa taaatcaaaa tttatcgcca atgacagtta
    10201 ttgcgaattt attaccaaca ggtaccgttt caggtgcacc aaaattacgt gcaattgaaa
    10261 gaatatatga acaatatcca cataaacggg gcgtttatag tggtggtgtt ggatacataa
    10321 attgtaatca taacttagat tttgcattag caattcgaac gatgatgata gatgagcagt
    10381 atatcaacgt agaagctggt tgtggcgttg tatatgattc tattcctgaa aaagaactga
    10441 atgaaacgaa attgaaagct aaaagcttat tggaggtgag cccatgatct tagttgtaga
    10501 taattatgat tcctttacat ataacctagt ggatattgtt gctcaacata ctgacgtcat
    10561 tgttcaatac cctgatgatg ataatgtgct gaatcaatcg gtggacgctg ttattatatc
    10621 tcctggtcca gggcatccat tagacgatca acagttaatg aaaatcatat caacctatca
    10681 acacaaaccc attttaggta tttgtttagg ggctcaggca ctgacttgtt actacggtgg
    10741 agaagtcatt aaaggcgaca aggttatgca cggcaaagtt gatacactaa aggttatatc
    10801 gcatcatcaa catctgttat atcaagatat accagaacag ttttcaatta tgagatatca
    10861 ttcattaata agtaaccctg acaattttcc agaagaattg aaaattactg gacgtaccaa
    10921 agattgtata cagtcattcg agcataaaga aagaccgcat tatggtattc agtaccatcc
    10981 tgaatcattt gctacagact atggtgtcaa aataattaca aatttcatta atctagtgaa
    11041 ggaaggatga aaaccatgac attactaaca agaataaaaa ctgaaactat attacttgaa
    11101 agcgacatta aagagctaat cgatatactt atttctccta gtattggaac tgatattaaa
    11161 tatgaattac ttagttccta ttcggagcga gaaatccaac aacaagaatt aacatatatt
    11221 gtacgtagct taattaatac aatgtatcca catcaaccat gttatgaagg ggctatgtgt
    11281 gtgtgcggca caggtggtga caagtcaaat agtttcaaca tttcaacgac tgttgctttt
    11341 gttgtagcaa gtgctggcgt aaaagttata aaacatggta ataaaagtat tacctcaaat
    11401 tcaggtagta cggatttgtt aaatcaaatg aacatacaaa caacaactgt tgatgataca
    11461 cctaaccaat taaatgaaaa agaccttgta ttcattggtg caactgaatc atatccaatc
    11521 atgaagtata tgcaaccagt tagaaaaatg attggaaagc ctacaatatt aaaccttgtg
    11581 ggtccattaa ttaatccata tcacttaacg tatcaaatgg taggcgtctt tgatcctaca
    11641 aagttaaagt tagttgctaa aacgattaaa gatttaggta gaaaacgtgc aatcgtttta
    11701 catggtgcaa atggtatgga tgaagcaaca ctatctggtg ataatttgat atatgaattg
    11761 actgaagatg gagaaatcaa aaattacaca ttaaatgcga ctgattatgg tttgaaacat
    11821 gcgccgaata gtgattttaa aggcggttca cctgaagaaa atttagcaat ctcccttaat
    11881 atcttgaatg gtaaagatca gtcaagtcga cgtgatgttg tcttactaaa tgcgggttta
    11941 agcctttatg ttgcagagaa agtggatacc atcgcagaag gcatagaact tgcaactaca
    12001 ttgattgata atggtgaagc attggaaaaa taccatcaaa tgagaggtga ataatatgac
    12061 gattttatca gaaattgtta aatataaaca gtcactttta caaaatggct attatcaaga
    12121 caaacttaat accttgaaaa gtgtgaagat tcagaataaa aaatctttta taaacgcaat
    12181 tgagaaagaa ccaaagctag caattattgc agaaattaaa tcgaagagtc ctacagttaa
    12241 tgacttacct gaacgagatt tatcgcaaca aatctcagat tatgaccaat atggtgcaaa
    12301 tgccgtgtcc attttaactg atgaaaagta ctttggtggt agttttgaaa gattacaagc
    12361 attgacgaca aaaacaacat tacccgtatt atgcaaagac tttattatag acccgcttca
    12421 aattgatgtt gctaaacaag ctggtgcatc tatgatttta ttgatcgtta acatcttatc
    12481 tgataaacaa ttgaaagatt tatataacta cgctatatcg caaaatctag aagtgttagt
    12541 tgaagtacat gatcgccatg aattagaacg tgcctataag gttaatgcta aattgattgg
    12601 tgtaaataac agggacttaa aacgatttgt tacaaatgtg gaacatacaa atactatttt
    12661 agaaaataaa aaaacaaatc attattatat ttctgaaagt ggtattcacg atgcatctga
    12721 tgtaagaaaa atcttgcata gtggtatcga tggcttacta ataggtgagg cgcttatgcg
    12781 ttgtgacaat ctatctgaat ttttaccaca actgaaaatg caaaaggtga agtcatgatg
    12841 aaattgaaat tttgtggctt tacatcaata aaggatgtta cagcggccag tcaattacct
    12901 attgatgcga taggtttcat ccattatgaa aaaagtaaaa ggcatcaaac aattacccaa
    12961 ataaaaaagt tagcgtctgc tgttccaaat catatcgata aagtatgtgt catggtaaat
    13021 cctgatttaa caacaattga acacgtatta agcaatacgt caattaacac aatacagtta
    13081 cacggcacag aatctattga ttttatacag gaaattaaaa agaaatattc aagcattaaa
    13141 atcactaaag ctttagctgc agatgaaaac ataatccaaa acataaataa atataaaggg
    13201 ttcgtagatt tatttattat cgacacaccc tcagtgtcgt atggtggtac cggtcaaaca
    13261 tatgactgga ctattttgaa gcacataaaa gacatacctt atttgatagc aggaggcatt
    13321 aactctgaaa atattcaaac agttaatcaa cttaaattat cacatcaagg ttatgatctt
    13381 gcatcaggta tagaagtaaa tgggcgaaaa gatatagaaa aaatgacagc aattgtaaat
    13441 attgtgaaag gagatagaga caatgaataa acaaatacaa acagaagcag atgaattagg
    13501 tttctttggt gaatatggag ggcaatatgt tccagaaaca ttaatgccag caattattga
    13561 gttgaaaaaa gcttataaag aggcaaaagc agacccagag tttcaaagag aactggaata
    13621 ctatttatca gagtatgtag gacgcgcgac accacttaca tatgctgcat catatactga
    13681 aagcctaggt ggcgctaaaa tatatttgaa acgagaggat ctaaatcata caggcgccca
    13741 taaaattaat aatgcgttag gtcaagcgtt gcttgctaaa agaatgggca agaagaagct
    13801 tgttgctgaa actggtgcgg gtcaacatgg tgtagctagt gctacggttg ctgcattatt
    13861 tgatatggaa cttgttgtct ttatgggaag tgaagatatt aaaagacaac aacttaatgt
    13921 atttagaatg gaattacttg gtgcaaaggt tgtggcagtt gaagatggtc aagggacttt
    13981 atcggatgca gttaataaag cattgcaata ttgggtaagt catgtagatg atacacatta
    14041 tttattaggt tctgcattag gtccagaccc gttcccaacg attgttagag attttcagag
    14101 tgtgattggt aaagaaataa aatcacagat attgaagaaa gaaggtcgac ttccggatgc
    14161 aattgtagca tgtatcggtg gtggctcaaa tgcaatcggt acattttatc catttattaa
    14221 agatgatgtt gcattatacg gtgttgaagc cgcaggtcaa ggcgatgata ctgataaaca
    14281 tgcacttgca attggcaaag gatcacctgg cgtattacat ggtactaaaa tgtatttaat
    14341 tcaagatgaa gatgggcaag tgcaactagc acattctatt tcagcaggac ttgattatcc
    14401 tggtattgga ccagaacatt cttattacca cgacattggt agagtaactt ttgaaaatgc
    14461 tagtgataca caagcaatga atgctttaat caactttaca aaacatgaag gtattatacc
    14521 tgcaattgaa agtgcacatg cactgagtta tgttgaaaga ctagcgccta cgatgtcgaa
    14581 agaagatatt attgtagtaa ctatttctgg acgtggcgat aaagatatgg aaacaattag
    14641 acaatatatg gtagagcgag gtcttgcaaa tgactaaatt atttatacct tatattatgg
    14701 gcaataaaga tttgattgaa aatgcaacat tgttgagtga aaatggtgca gatataattg
    14761 aaattggagt acctttctct gatccggttg ctgatggtcc agttatcatg gaagcaggtc
    14821 aacaagcgat taaacaaggc atcacgatag attatatttt caatcaatta gaaaaacatg
    14881 gtgatcaaat taagtgtaac tatgtattaa tgacgtatta taatattatt tgtcattatg
    14941 gagaacaagc gttttttgaa aaatgtcgag atactggtgt ctacggctta attattcctg
    15001 atttaccata tgaattatcg cagcgtttaa aacaacaatt tagtcactat ggcgtcaaaa
    15061 tcatatcgtt agttgcgatg actactgatg acaaacgtat aaaagatatc gtatcccatg
    15121 cggaaggctt tatttatact gtgacgatga atgcgacaac agggcaaaac ggtgcgtttc
    15181 atccagaatt aaaacgaaaa attgagtcaa ttaaagcgat agccaatgtg ccagttgtcg
    15241 caggatttgg tataagaaca ccacaacatg ttgcagatat aaaagaggtt gcagatggca
    15301 ttgtcattgg tagcgaaatc gttaagcgat ttaaatctaa cacgcgtgag gaaatcatta
    15361 aa
//
LOCUS       DAMQYT010000046        15130 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMQYT010000046 DAMQYT010000000
VERSION     DAMQYT010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15130
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.046"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..760
                     /locus_tag="PGA33_001100"
     CDS             <1..760
                     /locus_tag="PGA33_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDG6472925.1"
                     /translation="LGLLIGLFLYYLTQNWIVCFITSELFATIYTLVKLRGLTIGEYQ
                     SEDNNVVKDYVMLLSTNSLNNLNLYLDRLILLPIIGGTAVTISFLSTFIGKMLATFLY
                     PINNVVLSYISVNESDNIKKQYLKTNLIAIAALCLVMIICYPITIIIVSLLYNIDSSL
                     YSKFIILGNIGVLFNAVSIMIQTLNTKHASITLQANYMTLHTITFIFITILMTIAFGL
                     NGFFWTTLFSNIIKYVILNIIGLKSKFINKKDVD"
     gene            761..1966
                     /gene="cap8L"
                     /locus_tag="PGA33_001101"
     CDS             761..1966
                     /gene="cap8L"
                     /locus_tag="PGA33_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HDG6472926.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQSQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKALVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            1977..2534
                     /gene="cap8M"
                     /locus_tag="PGA33_001102"
     CDS             1977..2534
                     /gene="cap8M"
                     /locus_tag="PGA33_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HDG6472927.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            2534..3421
                     /gene="capN"
                     /locus_tag="PGA33_001103"
     CDS             2534..3421
                     /gene="capN"
                     /locus_tag="PGA33_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HDG6472928.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGHVGKSDQVDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSMLNKYFNKLSVFRKLFGNLIYSNTLYENNNALEIIPG
                     KMSLVIADIMDETTTKDKA"
     gene            3475..4737
                     /gene="cap8O"
                     /locus_tag="PGA33_001104"
     CDS             3475..4737
                     /gene="cap8O"
                     /locus_tag="PGA33_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HDG6472929.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPDASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILSFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTEACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKVLSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            4814..5959
                     /gene="cap8P"
                     /locus_tag="PGA33_001105"
     CDS             4814..5959
                     /gene="cap8P"
                     /locus_tag="PGA33_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HDG6472930.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVVLLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRTDKPDEFVPLRHK"
     gene            complement(6024..6350)
                     /gene="isdI"
                     /locus_tag="PGA33_001106"
     CDS             complement(6024..6350)
                     /gene="isdI"
                     /locus_tag="PGA33_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HDG6472931.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(6357..6740)
                     /locus_tag="PGA33_001107"
     CDS             complement(6357..6740)
                     /locus_tag="PGA33_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDG6472932.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRVGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
     gene            7167..8654
                     /locus_tag="PGA33_001108"
     CDS             7167..8654
                     /locus_tag="PGA33_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDG6472933.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGNPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            9300..10259
                     /locus_tag="PGA33_001109"
     CDS             9300..10259
                     /locus_tag="PGA33_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDG6472934.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEGIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(10321..10524)
                     /locus_tag="PGA33_001110"
     CDS             complement(10321..10524)
                     /locus_tag="PGA33_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472935.1"
                     /translation="MDKESVVASLARNKKIAVETMAGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDSIKEIDENHLNDAT"
     gene            10703..11215
                     /locus_tag="PGA33_001111"
     CDS             10703..11215
                     /locus_tag="PGA33_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HDG6472936.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            11557..12297
                     /locus_tag="PGA33_001112"
     CDS             11557..12297
                     /locus_tag="PGA33_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6472937.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGLQQKISDE
                     EINAQLKLVDLEDRGKHFPEQLSGGMKQRVALCRAHVHKPNVISMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            12311..13285
                     /locus_tag="PGA33_001113"
     CDS             12311..13285
                     /locus_tag="PGA33_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDG6472938.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKERSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            13282..14043
                     /locus_tag="PGA33_001114"
     CDS             13282..14043
                     /locus_tag="PGA33_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6472939.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWSFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            14056..15087
                     /locus_tag="PGA33_001115"
     CDS             14056..15087
                     /locus_tag="PGA33_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDG6472940.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNVQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
BASE COUNT         5203 a         2232 c         2852 g         4843 t
ORIGIN      
        1 tttaggtttg ctgataggac tatttctata ttatttaacc caaaactgga ttgtttgttt
       61 tattaccagt gaattgtttg caacgatata tacattggtt aaattacggg gattaactat
      121 aggcgagtat caaagtgaag ataataatgt ggtaaaagat tatgtgatgc tactgagtac
      181 aaatagcctt aataatttga atctctattt agatagatta atcttattac caattatagg
      241 tggaacagct gtaactatat catttctttc aacatttatt gggaaaatgt tagctacatt
      301 tctatatccg attaataatg tagtactttc atatatttct gtaaatgaaa gtgacaatat
      361 aaagaagcaa tatttgaaaa ctaatctaat tgctatagct gccctatgtt tagtcatgat
      421 tatatgttat ccaattacaa taattattgt ctctttactg tataacattg attcaagttt
      481 atattcgaag tttattattt taggtaatat aggtgtttta ttcaatgcag tgagtattat
      541 gatccaaact ttaaatacaa aacacgcatc aataacatta caagcgaatt atatgacgct
      601 tcacacgatt acatttatat tcataactat tttaatgaca attgcgtttg gtctaaatgg
      661 attcttttgg acaacgctgt tcagcaacat tattaagtat gtgattttaa atattatagg
      721 tttaaagtct aaattcatta ataaaaagga cgtcgattag atgagtgaaa aaaagatttt
      781 gattttatgt cagtattttt atccggaata tgtatcttct gcgacgttac caactcaatt
      841 ggcggaagat ttaattgcga atcacattaa tgtcgatgtc atgtgtggat ggccatatga
      901 atatagtaat cataaacagg tttctaaaac cgagatgcat cgtggtattc gcattcgacg
      961 tctcaagtat tcgaggttta ataacaaaag taaggttgga aggatcatca atttctttag
     1021 tttattttca aaattcgtga ttaatatacc taaaatgttg aaatatgatc agattcttgt
     1081 ttactctaat ccaccaatct tgccattaat accagacgtt ttacacagac tgcttaagaa
     1141 aaaatattct tttgtggtgt atgatatagc acctgataat gcgattaaga caggtgcaac
     1201 tcgtccaggt agcatgattg ataagctgat gcgttacatt aatagacatg tctacaagaa
     1261 tgctgaaaat gtcattgtcc ttggtacgga aatgaaaaac tacttactaa atcatcaaat
     1321 ttctaaaaat gctgacaata tccatgtgat tcctaactgg tatgacatgc gtcaattaca
     1381 agacaatcgt atctataatg acacatttaa agcttaccgt gagcaatacg acaaaatttt
     1441 attgtatagc ggtaatatgg ggcagttaca ggatatggag acacttatct catttttaaa
     1501 attaaataag gatcagtctc aaacgttaac aatactttgt ggtcatggta agaaatttgc
     1561 agatgtcaaa acggcaatag aagaccatcg tattgaaaat gttaaaatgt ttgagttttt
     1621 aacaggtaca gactatgctg acgtattaaa aattgcggat gtatgtattg catcgctgat
     1681 taaagaaggc gtcggtttag gcgtgccgag caagaattat ggctatcttg cagctaagaa
     1741 agcgttggta ctcatcatgg ataagcaatc tgatatcgtt caacatgttg aacaatatga
     1801 tgcgggtatc caaattgata atggcgatgc acatgccatt tataacttca tcaacactca
     1861 ctcgagtaag gaattgcacg agatgggtga gcgcgcacat caactgttta aagataaata
     1921 tacgagagaa attaatacta tgaagtatta caatctgttg aagtgaggag ataattatga
     1981 agcgattatt cgatgtagtg agttcaatat atggtttagt agttttaagt ccgattctgt
     2041 taattacagc attactaatt aaaatggaat cacctggacc agccattttc aaacaaaaaa
     2101 gaccgacgat taataatgaa ttgtttaata tttataagtt tagatcaatg aaaatagaca
     2161 cacctaatgt tgcaactgat ttaatggatt caacatcgta tataacaaag acagggaagg
     2221 tcattcgtaa gacctctatt gatgaattgc cacaattatt gaatgtttta aaaggagaaa
     2281 tgtcaattgt aggtcctaga ccagcgcttt ataatcaata cgaattaatc gaaaaacgta
     2341 caaaagcgaa cgtgcatacg attagaccag gtgtgacagg actagctcaa gtgatgggga
     2401 gagatgatat cactgatgat caaaaagtag cgtatgatca ttattactta acacatcaat
     2461 ctatgatgct tgatatgtat atcatatata aaacaattaa aaatatcgtt acttcagaag
     2521 gtgtgcatca ctaatgagaa aaaatatttt aattacaggc gtacatggat atatcggtaa
     2581 tgctttaaaa gataagctta ttgaacaagg acatcaagta gatcaaatta atgttaggaa
     2641 tcaattatgg aagtcgacct cgttcaaaga ttatgatgtt ttaattcata cagcagcttt
     2701 ggttcacaac aattcacctc aagcaaggct atctgattat atgcaagtga atatgttgct
     2761 gacgaaacaa ttggcacaaa aggctaaagc tgaagacgtt aaacaattta tttttatgag
     2821 tactatggca gtttatggaa aagaaggtca tgttggtaaa tcagatcaag ttgatacaca
     2881 aacaccaatg aaccctacga ccaactatgg tatttccaaa aagttcgctg aacaagcatt
     2941 acaagaattg attagtgatt cgtttaaagt agcaattgtg agaccaccaa tgatttatgg
     3001 tgcacattgc ccaggaaatt tccaacggtt aatgcaattg tcaaagcgat tgccaatcat
     3061 tcccaatatt aacaatcagc gcagtgcatt atatattaaa catctgacag catttattga
     3121 tcaattaata tcattagaag tgacaggtgt gtaccatcct caagatagtt tttactttga
     3181 tacatcgtca gtaatgtatg aaatacgtcg ccaatcacat cgtaaaacgg tattgatcaa
     3241 catgccttca atgctaaata agtattttaa taagttgtcg gtctttagaa aattattcgg
     3301 caatttaata tacagcaata cgttatatga aaataataat gcacttgaaa ttattcctgg
     3361 aaaaatgtca cttgttattg cggacatcat ggatgaaacg acaaccaaag ataaggcata
     3421 agtcatctat taaataaaat caacatacaa atcgttttat ttggaggtta tagtatgaag
     3481 ttaacagtag ttggcttagg ttatattggt ttaccaacat caattatgtt tgcaaaacat
     3541 ggcgtcgatg tgcttggtgt tgatattaat cagcaaacga ttgataagtt acaaagtggt
     3601 caaattagta ttgaagaacc tggattacaa gaggtttatg aagaggtact gtcatcggga
     3661 aaattgaagg tatctacaac gccagatgca tctgatgttt ttatcattgc cgttccgacg
     3721 ccgaataatg atgatcagta ccggtcatgt gacatttcgc tagttatgcg tgcattagat
     3781 agtattttat catttttaga aaaaggaaat accattattg tagagtcgac aattgcgcct
     3841 aaaacgatgg atgattttgt aaaaccagtc attgaaaatt tagggtttac aataggtgaa
     3901 gatatttatt tagtgcattg tccagaacgt gtactgccag gaaaaatttt agaagaatta
     3961 gttcataaca atcgtatcat tggcggtgtg actgaagctt gtattgaagc gggtaaacgt
     4021 gtctatcgca cattcgttca gggagaaatg attgaaacag atgcacgtac tgctgaaatg
     4081 agtaagctaa tggaaaacac atatagagac gtgaacattg ctttagctaa tgaattaaca
     4141 aaaatttgca ataacttaaa tattaatgta ttagatgtga ttgaaatggc aaacaaacat
     4201 ccgcgtgtta acatccatca gcctggtcca ggtgtaggcg gtcattgttt agctgttgat
     4261 ccgtacttta ttattgctaa agaccctgaa aatgcaaagt taattcaaac tggacgtgaa
     4321 attaataatt caatgccggc ctatgttgtt gatacaacga agcaaatcat caaagtgttg
     4381 agcgggaata aagtcacagt atttggttta acttataaag gtgatgttga tgatataaga
     4441 gaatcaccag catttgatat ttatgagcta ttaaatcaag aaccagacat agaagtatgt
     4501 gcttatgatc cacatgttga attagatttt gtggaacatg atatgtcaca tgctgtcaaa
     4561 gacgcatcgc tagtattgat tttaagtgac cactcagaat ttaaaaattt atcggacagt
     4621 cattttgata aaatgaagca taaagtgatt tttgatacaa aaaatgttgt gaaatcatca
     4681 tttgaagatg tatcgtatta taattatggc aatatattta attttatcga caaataaaat
     4741 gtgtcaaact agggcataca tgattaagga aagataagct gtcatgtgtt tgaacttcag
     4801 agaggataat gttatgaaaa aaattatggt tattttcggt acgagacccg aagcaataaa
     4861 aatggcacca ttagtaaaag aaattgatca taatgggaac tttgaagcga acattgtgat
     4921 tacagcacaa catagagata tgttagatag tgtgttaagt atatttgata ttcaagctga
     4981 tcatgattta aatattatgc aagatcaaca aacattagca ggccttacgg cgaatgcact
     5041 tgctaaactt gatagcatca ttaatgagga acaaccggat atgattttag tacatggtga
     5101 tactacaacg acttttgtag gaagtttggc agcattttat catcaaattc cggtcggaca
     5161 tgtagaagct ggacttcgaa cacatcagaa atactcacca tttcctgaag agttaaatcg
     5221 agtcatggta agtaatattg ctgaattgaa ttttgcgcca acagtaattg cagctaaaaa
     5281 tttacttttt gaaaacaaag acaaagagcg tatctttatt actggaaata cagttattga
     5341 cgcattgtca acaacagttc aaaatgattt tgtttcaacg attattaata aacataaagg
     5401 caagaaagtt gttttactaa cagcgcatcg tcgtgaaaat attggggaac cgatgcatca
     5461 gatttttaaa gcagtaagag atttggcaga tgaatataaa gatgttgtct tcatttatcc
     5521 aatgcatcgt aatccaaagg taagagcgat tgccgaaaaa tatttatctg ggagaaatcg
     5581 gattgaatta attgagccat tagatgcgat tgagttccat aattttacaa atcaatcgta
     5641 cctcgtgctg acagattctg gtggtattca agaggaggct cctacatttg gaaaacctgt
     5701 gttggtatta aggaatcata cagagcgtcc cgaaggcgtt gaggcgggaa catcgagagt
     5761 aattggcaca gattatgaca atattgttcg aaatgtgaaa caattgattg aggatgatga
     5821 agcgtatcaa cgtatgagtc aagcgaataa tccatatggt gatggacaag catcacgacg
     5881 tatttgtgaa gcaatagaat attattttgg attgcgcaca gacaagccgg atgaattcgt
     5941 acctttacgt cacaaataat aaaaaacccc taatcatgaa gttggtttag acaaccagcg
     6001 gtgactaggg gtttttaata tatttatttt tgatagtggt agccaatatc atatttgaat
     6061 actttatttg ataatattgg actttgctgt ccatcgtcat cactttttaa acgtacattt
     6121 ttatgagctt ctttaaatac atcggaattc aaccaattat taaagctatc ttcagattcc
     6181 caaatagtta agattttaac ttcgtctgta tcctcggtat ttaatgtttt agtgacaaac
     6241 atttgttgga agccttcaat agtttcaata ccttgtctat tgtaaaaacg ttcaatcgtt
     6301 tcttccgcac tgcctttttg taattgtaat ctattttctg ccataaacat gggcaatcac
     6361 tcctctattt tatgatttga tttgggtaat gtttttacaa atgtaaagag tacagcggtt
     6421 tgtatgataa ccattatgat taatcctaca cggactgcaa gaacatccac catataaatt
     6481 gaaaaaccta ttacaatgta taagctaatt aaaattttaa ttttctgttg tagcgtgtag
     6541 cctcgatgta aataaaagtt ttctacatat tctttataaa ttttttgatt aataagccaa
     6601 ttgtaaaagc gatctgaact tcgagcaaaa caaaaaactg ctacgagtaa aaaaggggtc
     6661 gttggcagta aaggtaatac ggcacctgca ataccaagcg ctgtaaatat taagccaatg
     6721 acgattaaaa taagtcgcat tgaaaaaact ccattctagt actaatgcgc atgtaatatt
     6781 gttttagtaa tataactcat gctaaatata atgtgtatga taagtgcaat gactcagtaa
     6841 aatgaaacga tgttgaatta tccttgtcac attaacgcat tttaagcgcg actttcataa
     6901 caaccaaact atttaatgag aattattctc aagtattata gttatattat gtgttttatt
     6961 tttgaaaagt gcaatatgtt ttcgaaaata agattatttt tatgtgcaaa aacgacgcaa
     7021 aagttttaaa aatgagactt ctgtgagctg attattttat aaaatgtaaa cgcttactat
     7081 ataatgtgaa tcatatcgtt taaaagcatt attaaatatg atgctaagag atttatatta
     7141 tagccaataa acaaaggaga gataatatgg cagtaaacgt tcgagattat attgcagaga
     7201 attatggttt atttatcaat ggggaatttg ttaaaggtag cagtgacgaa acaatcgaag
     7261 tgactaatcc agcaactgga gaaacactat cacatattac aagagcaaaa gataaagatg
     7321 tcgatcatgc agtcaaagtg gcgcaagagg catttgaatc atggtcatta acttctaaat
     7381 cagaacgtgc acaaatgttg cgtgatattg gtgataaatt aatggcacaa aaagataaaa
     7441 ttgcaatgat tgaaacatta aataatggta aaccgattcg tgagacaaca gcaattgata
     7501 ttccatttgc tgcaagacat ttccattatt tcgcaagtgt tattgaaaca gaagaaggta
     7561 cagtgaatga tatcgataaa gacacaatga gtatcgtacg acatgagccg attggcgtcg
     7621 taggtgctgt tgttgcttgg aacttcccaa tgctattagc tgcatggaag attgcgccag
     7681 ccattgctgc aggtaataca attgtgattc aaccttcgtc ttcaacacca ttaagtttat
     7741 tggaagttgc taaaattttc caagaggtat tacctaaagg tgttgtcaat atactaacgg
     7801 gtaaaggttc agaatcaggt aatgcaattt tcaatcatga tggtgtagat aaattatcat
     7861 ttacgggctc aactgatgta ggttatcaag ttgccgaagc tgcagcaaaa catctagtac
     7921 ccgctacatt agagcttggt ggtaaaagcg ccaatatcat attagatgat gctaatttag
     7981 accttgcagt tgaaggtatt cagttaggta ttttattcaa ccaaggtgaa gtatgtagtg
     8041 caggttctcg attattagtt catgaaaaaa tttatgatca attggtgcca cgtttacaag
     8101 aggcattttc aaatattaaa gttggaaatc cacaagatga agctacacaa atgggtagtc
     8161 aaactggtaa ggatcaatta gataaaattc aatcatatat tgatgcagca aaagaatcag
     8221 atgcacaaat tttagcaggc ggtcatcgct taactgaaaa tggattagat aaagggttct
     8281 tctttgagcc gacattaatt gctgtgccag acaatcatca caaattagca caagaagaaa
     8341 tatttggacc agtgttaaca gtgattaaag tgaaggacga tcaagaagca attgatatag
     8401 ctaatgattc tgagtatggt ttagcaggcg gtgtattttc tcaaaatatc acacgtgcat
     8461 taaatattgc taaagctgta cgtacaggac gtatttggat taacacttac aaccaagtac
     8521 cagaaggcgc accatttggt ggttataaaa aatcaggtat cggtcgagaa acttataaag
     8581 gtgcgttaag taactatcaa caagttaaaa atatttatat tgatacaagc aatgctttaa
     8641 aaggtttgta ctagaataaa tatcgtttct gaagcgtgtt tgtaggtcag tctagcggta
     8701 agtcttaaca tttaacggcg ttgtttagat tttaagcaaa acaaaatata taggaacacg
     8761 tatcatgata ttaggatata atgactaaaa taatagcagt aggatggttt ttaattgcaa
     8821 atcatcttac tgctgttttt aattatgcta atttgcgatg cggctattat aaggacagag
     8881 ttgtttatta attatggtga tttagaaata tgaagttcaa tatgcaaagt catcgtttgt
     8941 tttaatatgc ggaacaatca ttaaagttat tgcgattttt tgaacttaat gaaactaaac
     9001 aataaatttg agatactttt ttgtcatttt tatgtaacta acacaataat ctcgtacatt
     9061 attaaaattt tctatatgat aggaataaag caaagcgcga gtgtgctgta aaagttttcc
     9121 aaggtgatat tacataaagc tataaagggt aaagattaat gagttgtcat gtaaatgacg
     9181 atgatgtata aatcatggtt aattacggaa gcattaatat taacctgaga agctataaag
     9241 aattattttt aaaagcgaca atattaaata cgacgcattt atttaggagt ggcaaacgta
     9301 tgaatgggaa aaaggcgaat acgataaaca gatacaaata ttttcatcat gtcaatcatc
     9361 aaaaaattca acaaagttct aaaaagacgc tgtgggcatc actaatcatc acattgttat
     9421 ttacagtgat tgaatttgtc ggaggtttag tatctaattc attggcatta ctgtcagatt
     9481 catttcatat gcttagtgat gtattagcac ttggtttatc tatgttggcc atttattttg
     9541 caagtaaaaa gccgactgca cgatacacat ttggatattt aagatttgag atattagctg
     9601 catttttaaa tggtttagca ttaattgtaa tttcaatctg gattttatat gaaggtattg
     9661 tacgtattat ttatccgcaa ccaattgaaa gtggcattat gtttatgatt gctagtattg
     9721 gtttactcgt caatattatt ttgactgtta tccttgtaag gtctttaaaa caagaagaca
     9781 atatcaatat tcaaagtgca ttatggcatt tcatgggaga cttattgaac tctattggtg
     9841 tcatcgttgc agttgtattg atttacttta caggatggcg catcatcgac ccaatcatta
     9901 gtattgtaat ttcactcatc attttacgtg gtggttataa aattacgcgt aatgcgtggt
     9961 taattttaat ggaaagtgtg cctcaacatt tggatactga tcaaattatg gcagatatta
    10021 aaaacataga tggcatatta gatgtacatg aatttcattt gtggagtatt acaacagagc
    10081 attattcatt aagtgcccat gttgtgttag ataaaaaata tgagggtgat gattatcaag
    10141 cgattgatca agtatcatca ttgttgaaag aaaaatatgg cattgcacat tcaacgttgc
    10201 aaattgaaaa cttgcaattg aatccattag atgagccata cttcgacaaa ttaacataaa
    10261 taaaacattg tagcgcctaa aacattaatc tatgtcatag gcgcacgttt cgttttatac
    10321 ttatgttgca tcatttaaat gattttcgtc aatttctttg atgctatcta catctaacac
    10381 gacatcttta ggtttcaaaa tatgaatatg tttttcatca tttgtatgta aaatgcgttc
    10441 tatgatgtac ctttgaccgg ccattgtttc tacagcaatc tttttgtttc tagctaaact
    10501 tgctacgaca gattctttat ccataatgat agccccctat atatatgttt atttacttat
    10561 accctaacat gatttttata ctctttgaaa atatatttta cagaatttta tctaaatatt
    10621 taaaaaaata tcttaatatc cttgtaatcc gataagaatt atagtaatat tttttcaacc
    10681 attgttatag gaggtcttat taatgacatt atttttatta gaagctaaca atcttgattt
    10741 tgcatcaacg aaagaagaac tagaagcaaa ggcagcatca ctatctacga agacaattcc
    10801 aacattaatt gaagtacaag ctactgaaaa tttaactcat ggttatttta ttgtggaagc
    10861 aaatgacgaa gcagaagcta aacaattttt aacagaagca gatattagta ttcaattagt
    10921 caaagaagta cgcttagttg gtaaagattt agatgaagtt aaaaatggtg atgcacatgt
    10981 tgattacctt gtaacttgga acattccgga aggcattacg atggatcaat atttagcacg
    11041 taaaaagaaa aattctgttc attatgaaga agtgccagaa gttgaattta aacgcacata
    11101 tgtatgtgaa gatatgtcta aatgtatttg tttatacaac gcacctgatg aagaagcggt
    11161 acgtcgcgcg cgcaaagcag ttgatacacc gattgatggc atcgaaaaac tttaataaga
    11221 caacaagttg atgagatata tgtatatagg tttggcatgg atttcgattg cagttaatta
    11281 gaatagctca atgctataaa tgtaagtagt tgatatgaag aaactaatga actaaatgca
    11341 agtattgtct aaaacaatca ttttattgaa atttagtaga gctgaaatta atataacgtc
    11401 gttaattgaa taacgcttat gttataagag cactcatacc aaaccataat catctataga
    11461 tataacaatt cacgatataa gggctgtgtt tggcatagcc ctttagatat acacttaatt
    11521 cctattaaaa tagtaggggt taaaaggggg cttgtcatga ttaaaattca acaattacaa
    11581 catcactttg gatcacataa agtaattcat aactttaatt tggacattag caagggagaa
    11641 atagtcactt tcatagggaa aagtggttgc ggaaagtcta ctttactcaa tattatcggt
    11701 ggatttattc atccatcgtc tggtcgtgtc attattgata acgaaattaa acaacagcca
    11761 tctccagatt gtttaatgct atttcaacat cataatttgc tgccatggaa aacgattaat
    11821 gacaacatta ggattggatt acaacagaaa attagtgatg aagagattaa cgcacagctt
    11881 aaattagttg atttagaaga caggggaaag cattttcccg agcaactgtc cgggggtatg
    11941 aaacaacgtg tggcactatg tcgagcgcat gtgcataagc ctaacgttat atcgatggat
    12001 gagccattag gtgcattaga tgcatttaca cgttataaac ttcaggatca actagtgcaa
    12061 ctaaaacata aaacgcaatc aactattatt ttagtgacgc atgacattga tgaagctatt
    12121 tatctttccg accgcattgt tctgttaggt gaagggtgca atattatttc tcaatatgaa
    12181 attacagcat cacatccacg cagtcgtaat gatagccacc tacttaagat tcgtaatgaa
    12241 attatggaaa catttgcatt gaatcatcat caagttgaac ctgaatatta tttataagga
    12301 gtgagtgacg atgaaaaggt taagcataat cgtcatcatt ggaatcttta taattacagg
    12361 atgtgattgg caaaggacgt ctaaagaacg gtctaaaaat gcccaaaatc agcaagtgat
    12421 taaaattgga tatttgccga ttacacattc agctaatttg atgatgacta aaaaattatt
    12481 atcacaatac aatcatccga aatataaact agaattagtt aaattcaata attggccaga
    12541 tttaatggac gcattaaaca gtggtcgtat tgatggtgca tcaactttaa tagagctagc
    12601 gatgaaatca aaacagaagg gctcaaatat aaaggctgtg gcattgggcc atcatgaagg
    12661 caatgtcatt atgggacaaa aaggtatgca cttaaatgaa tttaataata atggcgatga
    12721 ttaccatttt ggtataccac atcgttattc aacacattat cttttacttg aggaattacg
    12781 taaacaatta aagattaaac cggggcattt tagctatcat gaaatgtcgc cagcagaaat
    12841 gccagccgca ttgagtgaac acagaattac agggtattct gtagccgaac cattcggtgc
    12901 actgggtgaa aagttaggca aaggtaagac tttgaaacat ggtgatgacg ttatacctga
    12961 tgcgtattgc tgtgtgctag tactgagagg ggaattgctt gatcaacaca aggatgtagc
    13021 gcaagcattt gtacaagatt ataaaaagtc tggctttaaa atgaatgatc gcaagcaaag
    13081 tgtagacatt atgacgcatc attttaaaca aagtcgtgac gttttaacac agtcagcggc
    13141 atggacatcc tatggtgatt taacaattaa gccatccggc tatcaagaaa ttacgacatt
    13201 ggtaaaacaa catcatttgt ttaatccacc tgcatatgat gactttgttg aaccgtcatt
    13261 gtataaggag gcatcgcgtt catgacacgt cccacaaata acaaatttat attacctatt
    13321 atcacattta ttattttctt aggcatttgg gaaatggtca ttattattgg gcattaccaa
    13381 cctgtattgt taccgggtcc tgctcttgta ggaaaaagta tatggtcttt cattgttact
    13441 ggagaaattt tccaacattt agcaattagt ttatggagat ttgtagcggg ctttgttgtc
    13501 gcattgttgg ttgctattcc attgggcttc ttgcttggaa ggaatcgttg gctatacaac
    13561 gctatcgaac cgctatttca attgattagg ccgatatctc cgatagcatg ggcaccattt
    13621 gttgttctat ggtttggtat tggtagtttg ccagcgattg cgattatttt tatcgctgct
    13681 tttttcccaa ttgtgttcaa tactattaaa ggcgttagag acattgaacc tcaatattta
    13741 aaaatagcag caaatttaaa tttaactggg tggtcattgt atcgcaatat attatttccc
    13801 ggggcattta aacaaatcat ggctgggata catatggcgg taggaacaag ttggatattt
    13861 ttagtttctg gtgaaatgat tggtgcacaa tcgggattag gttttttaat cgttgatgca
    13921 cgaaatatgt tgaacttaga agatgtttta gcagcaatat tctttatcgg attatttggt
    13981 tttattattg atcgattcat tagttatatt gagcagttta tacttagaag atttggtgaa
    14041 taaggagaga tgatgatgac tttagaaacg cttatcaaag aacaattaga tcctcattta
    14101 gtagaagttg atgaagggac gtattatccg agaacattta ttcagcaatt atttgtagat
    14161 ggttatttcg gtgaggcggc attgagaaaa aatgctgaag taatcgaagc tgtatcgcag
    14221 tcttgtttga caacaggatt ttgtttatgg tgccaattag ctttttcaac gtatttagaa
    14281 aatgccacgc agccacattt aaataatgac ttacaacagc aattgttatc tggagaaata
    14341 ttaggtgcta ccggattgtc taatccgatg aagtcattta atgatttaga aaagttgaac
    14401 cttgaacaca cttatgttga tggacaattg gttgtcagtg gacgtatgcc agctgtaagt
    14461 aatattcaag aagaccatta ttttggtgcg atttcgaaac atgaatcatc agatgaattt
    14521 gtcatgttca ttctacgtgc caatcaagat ggtatcactc ttgttgaaaa aaccaatttt
    14581 ttaggagtca acgggtcagc aacgtatcaa atcacattga atcaagtcgt agtgccacaa
    14641 tcacaaatta tcacgcatga tgcgaagcag tttgcggcaa ctattcgccc gcaatttatt
    14701 gcttaccaaa ttccaatagg attaggctca attaaaagtt ctttagagtt aattgatgca
    14761 ttttcaaatg tgcaaaacgg aataaatcaa tatttagagt atgatgttga agcttttaaa
    14821 aaacgttatc gtcaacttag agaggaatat tatgcaatat tagatgacgg taacttaact
    14881 tcacatttaa atgaattaat atcattgaag aaggacatcg gctatttatt gttagatgta
    14941 aatcaagctt ctgttgtcaa tggtggttct agagcgtaca caccatattc gccacaagtt
    15001 cgcaagttaa aagaaggatt cttcttcgca gcattgacac cgacattaag acatttaggt
    15061 aaacttgaag cagagttgaa ggggtaagtg tgataagctg attttttgtt tagatgcgtt
    15121 tgttgaaaca
//
LOCUS       DAMQYT010000047        15116 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMQYT010000047 DAMQYT010000000
VERSION     DAMQYT010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15116
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.047"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(195..365)
                     /locus_tag="PGA33_001116"
     CDS             complement(195..365)
                     /locus_tag="PGA33_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472941.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(427..525)
                     /locus_tag="PGA33_001117"
     CDS             complement(427..525)
                     /locus_tag="PGA33_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472942.1"
                     /translation="MIKKKHLSGSLVPDRCFLIISEEVERFMENAY"
     gene            complement(544..1443)
                     /gene="sdaAA"
                     /locus_tag="PGA33_001118"
     CDS             complement(544..1443)
                     /gene="sdaAA"
                     /locus_tag="PGA33_001118"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDG6472943.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            complement(1457..2137)
                     /gene="sdaAB"
                     /locus_tag="PGA33_001119"
     CDS             complement(1457..2137)
                     /gene="sdaAB"
                     /locus_tag="PGA33_001119"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDG6472944.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            complement(2140..3183)
                     /locus_tag="PGA33_001120"
     CDS             complement(2140..3183)
                     /locus_tag="PGA33_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDG6472945.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            3734..3937
                     /locus_tag="PGA33_001121"
     CDS             3734..3937
                     /locus_tag="PGA33_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472946.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            4120..5055
                     /locus_tag="PGA33_001122"
     CDS             4120..5055
                     /locus_tag="PGA33_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDG6472947.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPETLDE"
     gene            complement(5137..6105)
                     /locus_tag="PGA33_001123"
     CDS             complement(5137..6105)
                     /locus_tag="PGA33_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDG6472948.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVKVDKHFGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(6238..6582)
                     /locus_tag="PGA33_001124"
     CDS             complement(6238..6582)
                     /locus_tag="PGA33_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDG6472949.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEH"
     gene            complement(6624..7034)
                     /locus_tag="PGA33_001125"
     CDS             complement(6624..7034)
                     /locus_tag="PGA33_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDG6472950.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(7414..9480)
                     /locus_tag="PGA33_001126"
     CDS             complement(7414..9480)
                     /locus_tag="PGA33_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HDG6472951.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGIGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(9780..11519)
                     /locus_tag="PGA33_001127"
     CDS             complement(9780..11519)
                     /locus_tag="PGA33_001127"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDG6472952.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAIASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(11566..12255)
                     /locus_tag="PGA33_001128"
     CDS             complement(11566..12255)
                     /locus_tag="PGA33_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDG6472953.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(12248..12643)
                     /gene="cidA"
                     /locus_tag="PGA33_001129"
     CDS             complement(12248..12643)
                     /gene="cidA"
                     /locus_tag="PGA33_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HDG6472954.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(12856..13734)
                     /locus_tag="PGA33_001130"
     CDS             complement(12856..13734)
                     /locus_tag="PGA33_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDG6472955.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            13901..14119
                     /locus_tag="PGA33_001131"
     CDS             13901..14119
                     /locus_tag="PGA33_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDG6472956.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            14508..14939
                     /locus_tag="PGA33_001132"
     CDS             14508..14939
                     /locus_tag="PGA33_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDG6472957.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
BASE COUNT         4686 a         2902 c         2256 g         5272 t
ORIGIN      
        1 cgtcgtccca ccccaactcg cacattattg taagctgact tttcgtcagc ttctgtgttg
       61 gggcccaaaa agcttgttac aagcgcattt tcgttcagtc aactactgcc aatataactt
      121 tgtagagcat attacattga tttacattgt cccttttatt tattcttttc aaatactatc
      181 cccatagctt tgatttaacg ctttttctca ataacaaaac gaatatagta gaacatgaaa
      241 acgataatca tgctgagcga taaagattta aataatagat tgacccacgt tccctcagtc
      301 gtatatccat atgtaatcgt tgtgttaatg atgaatgcta taaagatgat tgatagtctt
      361 agcatatcat cactcctttt aagttatttt agatatacgg gggcgctttt gcaatcacta
      421 ttttgattag tatgcatttt ccataaatct ttcaacttct tcagagataa ttaagaagca
      481 tctatctggt actaatgatc cagacagatg cttctttttt atcaatattt tattgttatc
      541 tcattaatta tttttaacca tatcttcagc tgtgccaaag attttacgtt taattgcttc
      601 gccagttggt gtgcctgcta gtccacccaa tccagtttca cgtaatgatg caggaaggtt
      661 acgaccaacc ttatccattg cttcaataac ttcatcaaca gggattctac tttcaatacc
      721 tgctaatgct aaatctgctg aaattaaagc gttacccgaa ccaattgcat ttctcataac
      781 acaaggaatt tcaacaagtc cggctactgg atcacaaact aaacctaata aattacttat
      841 cgctaatgcc atagcgtgcc cggatgcttc tggtgatcct ccgaatatag ctactgctgc
      901 agctgcggcc attgcagatg ctgaaccaac ttcagcttgg cagccacctg ttgcaccagc
      961 tacacttgca ttgtttgcta cgacacgccc aaacaatgct gaagtgaata agaaatcaat
     1021 catttgctct tctgttaaat catgtgtttt ttctaattta aaaagtgcac cgggaatggt
     1081 acccgaggaa ccagctgttg gcgttgcaca aataataccc atcgcagcat tgacttcatt
     1141 tgttgcaatg gcacctttga ctgcgtcaat catttcatat ccagacaaag catgatgtgt
     1201 ttcattataa tcacgtagtt tagcagcatc atgaccagtg tagcccgtta cactttcaac
     1261 cccatcacct gtcgtccctt tgattactgc gtctcgcatg acatctaaat tttgtttcat
     1321 ttgcgctcgc acttcatcac gtgatttacc gcttaattcc atttcttctt taaccatgat
     1381 atccgcaaat gacatattat tttctacggc ataatctata gtctctctaa ttgaatcaaa
     1441 catgtttatt ccccctctaa tttatatagg aaacgtttac gtcactgtat ttctctttaa
     1501 ttgtatttaa tgttgattct gagattgctt tatttaatgg tattacaacc aagcatttat
     1561 cttcatctat cttaataaat tcatctttac agtctaattt catatcgttg atatcattga
     1621 tgaaatgatt tacttgtgct ttagtcatat ttccgtcaac aactaaaatt ggtaatccat
     1681 gatttaaatc tacttctagt ccatttatat gaataccttt aattttaatt gtaccaccac
     1741 cgattgaaat accgataatt tcaatgtagc gaccatcatt acgagatgat ttgatataag
     1801 cacagtttgg atgttgacca atactatcgc cttcttcttc gatgatatct attttaatac
     1861 catcatcagc tgcaatttct aatgaagatt taattcggtt atcaaatgtt gaatatccca
     1921 ttgctccacc cacaatagcg acatctgtac catgtccttg gtgtgtttga gcaaatgatt
     1981 cataataatg tatttcaata tttttaggaa tatctcccaa tattgcgcgt gctgaattcc
     2041 caatctttac tgcaccagcc gtatgagaac ttgaagggcc catcataact ggtccgataa
     2101 tatcgaaagc actttgataa tcatagctct ttgccataat taaacactct ccttaatatg
     2161 attctttttg ctcggcattt taaagttgat attcattaaa ttaaacttat taattagtgt
     2221 tccaataata taggctaaga caatgctgac aataatcact gttgcgattg ttacaattga
     2281 tgtcactgca ttattaaagc caaacaatac gatggcgcct gcaattggtg ttgccatacc
     2341 tttgacacct attactagtc cgctaaatgt cacgatacat gcgttgacga ccccaatcag
     2401 tgcatttgta ccatatagtt gtactggata ttgcgctatt aaatcaattt gcgtcaatgg
     2461 ctcaatacaa actgcaaatg cttttgacgg tccaccaatg tttaattttc ggaataaaat
     2521 aaggttaaca aatgagctac ctgtacatgt tagtgctcca atagccatag gaacacctgt
     2581 cagtcctaat aaacttgtta ataccattga acttagcggt gtcatacctg taacaggaat
     2641 cactagtcct aaaatgaccg ctaatgcata tggattgtta tcacctaccg cagtgacagc
     2701 actacctatt tgttttaatg ttgctagcac accaggtgta atgattgatg caagtccgaa
     2761 agcaattgct ggtgcaaata agatcaccac aattaagtcc aagccttctg gaactttctt
     2821 ttcaatccat ttaattaaaa aagctacgcc ataagctgcg atgaatgctg gtaataattt
     2881 aaagtcatgt aatactaaac caacaatgac cgcaaatact ggtgcaacgc ctaagtttaa
     2941 gcacgttaga atacctactg cgataccgct taaacttcct gctaaatccc caatatcttg
     3001 tagaaattta atatcaaata cgccaccaat agcataactt aagaatgctt gtggtagaaa
     3061 tgtcgcacaa gctgcaccgg ataatgcttg tagtccttgt ttaccgtacg gtgcatactt
     3121 taaaaatagc gtcatgatca ctaaaaccaa gactaatgtg cctacaccta acagaatatc
     3181 catttcccaa aaacctctct ctatgtttat tttattttca gaccataaac atcgtacacc
     3241 cttaagaaaa cgttttcaac ttttatctgt tatcaaatca aatatttaag tgaaatattt
     3301 gatatttgtg aaagattttt aaaatggatt gtttcaaaaa ctacttatgt tgtcgttaat
     3361 atttactaat taacttttta ctctatattt caaacagttg tgtgacagtt ttttgataac
     3421 ttttttacat ctgaaagtaa gtaatatttc taaaaacttt taatatttat acactttatc
     3481 tttcgagcta tttatttttt gtttttgtgc tgtgtttata aaaccgcaca ctatattcaa
     3541 aaataactta ttacacatat atttgcaata agataattaa agtaggatat tatttttagt
     3601 tttctgatag gaatgatgat agtctatagg ttgaatcttt actttttttt aaagctaaat
     3661 ttacatcaac ttaacaatgg ttggttatac tgaagatgaa aatatttagt ataacttagt
     3721 ggaggcgata aaggtgcaat tgagtcattc cgttaaagtt gcaatttcta tctatttagc
     3781 acttatcttt ataacgttca cttcttattt agtcattatt ttatatacga gtatgactgg
     3841 acatgatgta tcacatttcg tgttagatag tcagcattct catcatggat ctcttacgca
     3901 aaaacatttg agtcttcctg aaatctcatt taaatagttt atcctctgtg tttcaacatt
     3961 catttcccat atcgattcat ttatctatca tctagaccac tacatcttag atgatttttt
     4021 tattttctca tttcactctt tcttaaaagt cgatataatg aattaaatca ttatcataca
     4081 ccgacatatt ttatgttgtt ggtgttaagt tttaaagggg tgagatactt ggcgaataat
     4141 cattcagctt tgcgtttaaa aggaattata cttgccattg tcggtgcttg tttatgggga
     4201 ttaggtggta ctgtttctga tttcttgttc aaatataaga atattaatgt cgattggtac
     4261 gtcactgctc gacttgtagt cagtggtgtt ttcttactta ttatgtacaa aatgatgcaa
     4321 cccaaacgtt caatatttag cgtattccaa gatcgacgta tgttaggcaa attacttatc
     4381 ttcagtatac tgggcatgtt agtagtacaa tatgcttata tggcatctat taatacaggt
     4441 aatgctgcga ttgcaacatt actacaatac attgcgccag tttatattat tatttggttt
     4501 gtcataagag gcgttgcaaa actaacatta tttgatgtgc ttgctattat catgacacta
     4561 ttaggaacat ttttattatt aacaaatggt tcattttcta atttagtcgt caatcctgca
     4621 agtttattct ggggtatttt agctggtgta gcactcgctt tttacacaat ttatccttca
     4681 gacctactta accgcttcgg ttcgattcta attgtcgggt gggcaatgct tatttctggt
     4741 gttgcgatga atttacgcca tccaatttgg cacattgata tcactaaatg ggacatatca
     4801 attatattat ttttaatctt tggtattatc ggtggtaccg cactcgcatt ttatttcttt
     4861 atcgacagtt tacaatacat atcagcgaaa gaaacaacat tattcggaac tgttgaacct
     4921 gtcgtagccg ttatcgcaag cagtctatgg ttacatgtgg cattcaaacc atttcaaatc
     4981 gtaggcatca ttcttattat gattttaatt ttattactat cacttaaaag acaacctgaa
     5041 acattagatg aataagaaaa ctctgataat cactttagca agtaactatt atttaacaac
     5101 gtagttacct tataggtgat atcagagttt tttattttag ttaataatat ttttcacttg
     5161 gtataaaaag cgtcgtcgct ctggtatcgg aaatactgga taaatatgga attggtaata
     5221 tccaggtata aagtcatgtt cgataccttt cgcactcaac aattgcgata agttcaaagc
     5281 atctgggtac agtacttctt ttgtaccaac agttaatgta atacgaccta aaccatctaa
     5341 gtcaccatta attggtgata ctttgtagtt atctaaaggt gtgtcccctg cccattgttc
     5401 agctaagaac acactgccat ccacacctac cattgggtct ttctttaagt agtcaggaat
     5461 ttcaggatgc tgcatcgttg catctaaaac tggtgaaatt aatacaatat gtcctggttg
     5521 cacaatatgt ttttctttta acaattgagc aaatgataaa gcaatttgac cgcccgcaga
     5581 gtcacccatt acaacgattt gtttagaatc tgctacttga ttcaataaat catggtacaa
     5641 cttttcaaaa agcacatacg tcgcttgata atcttgatgc ggaatcttcg gatatactgg
     5701 catgatgact ttagcattga gtgtttctgc aagttcatca ataaattcga aatgaatttt
     5761 gagtgggtct tggaaccatg cgcctccatg tgcatataat acaacgcgtt gatgcttatc
     5821 ttgtttatcg ttaacggtat aaacggttga accgaagtgc ttatccactt ttactggcat
     5881 cttaaactga taattggttc cctcatattc gcctgctgtc tgtggcgcca ttttttctaa
     5941 tgctttcatc gcttcttctt tatacataaa acgacgctta gctctagata atcgtatacc
     6001 ttgttctaac atataacttt tcacactgcg tttttcttta attctaatat gtgcgtatgc
     6061 tgcaactaaa aatccaaacg caagtgtaga ccatttttta cgcatttgtc gtcactcctc
     6121 tttgatgatt tctactatag ttctactgta ccatgctgat tctaattact accaatattt
     6181 actgggaaat aagttatgat gtctacataa tcttagcaat gctaatcatt tgcgatatta
     6241 atgctcatcg acatcgttat ttacactatc attcatttgc ttaagatgat gttcaaaacg
     6301 ttgcatatcg ataaaacgcc cttgacgatg catttctttt ccattcataa aaattaaatc
     6361 cacaggtaca gtgaaaatat ttaattctcc agcaatagct tccacctgac tttgattaat
     6421 cacagctaat ggcacattgg gatatgattg catcaagtct tcaatttgtg gtaaaacggc
     6481 atgacacacg ctacactgat cgcgcatgac atgtacaact gctaacggat attgatgaat
     6541 aaattcacgg aatgcttcga cactatttaa ttgcttaacc atttcgaaca ccttcttact
     6601 ttttatattt taaaatgaag tcattatttt gttgctaaaa tgtcttgaaa cacttgtggc
     6661 acagaggtta tcttcttatc actcaatctg taaaaaccgc ctgtctgtgt accaatacaa
     6721 tgcacgccat gtgcggacac aatttcaaac tgaaacaccc atttcatctt gcttgcttta
     6781 actaagtaaa tacgacctac tggtttgtca tgtattgtga caggtacttt atactctgct
     6841 tccgttttca taagtatagg tgaaatgcct tgattgatca ttgttgagta attcatgtgc
     6901 tgattaataa acgctgttct caaatcttcg aaccatctaa tataaacgat attactaacg
     6961 acacccatcg catcaatgtc gtaaccattc acttcgattt ctttctctgc aactaataga
     7021 ccttctgttg tcataatgac aaatctcctt atcttacaaa tagatttaaa atcatgttaa
     7081 tttattttta ttactttcat gataaaaata caagcaattg aactttatta cggaaaatca
     7141 aacttttcaa tgagactatt aatttggcta gtcgcgaaga tgcatttttc taaaatgtga
     7201 caaaaacata agcaatgttt tctatatcta acactaatat ttaacttatt atcatcaaca
     7261 cacctgctat acatcattgc gcactaaaaa ggcagtagct tcattttaag ctactgcctt
     7321 ttgtaacgta ttctctttca actcgctact tcgcatacgc actacgaatg actaaaagtt
     7381 cattcatttg atatgctgtt tcatttttaa tcattatttg actgtcataa ttagcttacc
     7441 tggatcagca tcttgtacat cttcaatgac aagttcttta ttttcaagat ttgtaataat
     7501 cattggtgta acgatgcttg gtgcgtgtgc tttcaagtat gctaaattca ctttcattaa
     7561 tggttgacct tgtgttactt tttcatcaac gttaatcaga ctttcgaatc cttcaccatt
     7621 cagtttcact gtatcgatac caatatgaat aagtacttcg acgccacttt cagattctaa
     7681 tcctatcgca tgtttcgtag ggaagattgt tttcactgta ccatcaaacg gtgctacaat
     7741 ttcacctttt tcagggataa agccaacacc atcacccatc attttaccag cgaacacttg
     7801 atcaggtact tctgataatg gaatgatttg accgatacct ggtgcataga tgtctgttgt
     7861 tccaagtgct tctacgtgaa ctggttcatc tgacatttct tcagtcactg tcgtttcact
     7921 tggtttcgta atttcaccac tcataatctt ggccatatca tgtttaattt gatctgattt
     7981 tggaccaaag atagcttgca tattgtttcc aacttctaat acacctgatg cgcctaaagc
     8041 tttaatacct gctacatcta cttttgattt atcaaccact tctacgcgta gacgtgtaat
     8101 acatgcatct aaatgtttaa tgttttcttt tccacccatt gcatctaaga catcaaatgg
     8161 taattttgcg acactagagt tacgaatttc agtttcttca tcttcacgac ctggtgtttt
     8221 caatttaaac ttacgaattg caaagtcgaa taagaagtaa tacacgatag catatacaat
     8281 accgactgga ataactaata atgcgtgtga acgatcccag tttaataaac catataaaat
     8341 ataatctatg aaaccacctg agaatgtcat accaatttta acgcctaata aatgcattac
     8401 taagaatgat gtaccagcta ataatacgtg aattccataa agtactggtg ctacaaataa
     8461 gaatgaaaat tctaatggct cagtgatacc agttaaaaat gcagttaatc ctgctgataa
     8521 cattaaacca cccacgactt ttttacgttc tggtcgtgca tttttataaa tagcaaatgc
     8581 cgccgctggt aaaccaaaca tcataaatgg atatttacca gtagtaaatg caccagcagt
     8641 aaatggtacg ccatctttca attgtgccat ccaaatacgt tggtcaccac gaaccaattc
     8701 acctgcgtga tttgtataac ttccgaattc aaaccagaac ggtgaataga aaatatgatg
     8761 taaaccaaat ggaattaatg agcgttcaat aataccgaat ataaacgttg ttaatgttaa
     8821 atttttattt aataagaaat tcgataaact atttaatcca tcttgaattg gtggccacgc
     8881 aaagcttaaa agcacacctg ttgcgattgc tacgaccgat gtcacaatcg gtacaaatcg
     8941 tttacctgca aagaatccta aaaatggtgg tagtgtaata ttataaaatt tgttgtaaca
     9001 ccatgcggct aaagcaccca tgataatacc accaaacaca cccgtttgta acgttggaat
     9061 acctaatact aaagcatgtg ctggttcttt cgctgcttga cttaattctt ttgccccttt
     9121 ggcatatgag aaaatgtcat caattgtaat gtgcaacact ttccccattg ttgcattcat
     9181 aattaagtaa cctactagcg ctgctaatgc tgcaacaccg tctcctcctg ctaatccaag
     9241 tgctgtacca actgcaaata ataatggcaa gttatcaaat acaacttgtc ctgctgcttc
     9301 catgaccgac gaaatcatta caatgatatc gttttttaac catggtgcaa tttctactaa
     9361 ttgttcgttg tgcattgcgt taccaaacgc taataaaata ccagctgctg gtaaaatcgc
     9421 aacaggtaac attaatgctt taccgatacg ttgcaattgt ccaaacaatt tcttaaacat
     9481 ataaacatcc cctatctcaa tttccgcttc catgtacaaa aaaagcaggc atgaacaaac
     9541 aagactattc tcatagtgcg gttgtttgct catgctgcat taccagtaac acgcaagtga
     9601 tatctaattc atagctatta tacatatttg taaaagcgtt ttcaacaact ttttcacaat
     9661 tttatcatca attcagtacg atcatttgtg ataaataaac aaaacggcaa ttcccatttt
     9721 gttgaaatag aaattaccgt ttttatagtt caattcaatt gtgataacct ttaaatcagt
     9781 tataagaaac gttttgctgc cactgaaatt ggtggaattt gatctaaatc taaatgttta
     9841 tcttcagtaa ttgatctaaa tgcccactta ccataaccaa gcgcttcttc atttacaatt
     9901 ttacctggta atggcgcagc attaggatca acatatacat ctacaatcgt tggtacatct
     9961 tgtgctaatg cctcttcgac tatagcatct acttcgctag cactcttaat tgtataacct
    10021 ttaccacctg ctgcctcagc aaattttgca tgatccatat cagaaaaatc aactgcatat
    10081 tctaattcac cagctgcttg ttgttcatat ttaataaatg ctaactgttt gttattaagt
    10141 acaaatacag ttaaaggtaa atcatattgt actgctgtag cgaagtcttg cattaccatt
    10201 tggaatgcac cgtcaccagc aattgcgatg gcttgtctat ttggatatgc aatttttgat
    10261 gcaattgcac ctggaagacc gcaacccatt gtacctaacc aacttgaaat gatgaacttg
    10321 ttatttacac caaggtttaa gtatcgagtt gaccaaactg ttgctgtacc tacatctgct
    10381 gaaatcactg catcatcttt aataaattta ttgattgatg ccattaatcg ttctggacgt
    10441 aatggtttac tattattatt tttatcttgt tccatccatt tatcccaaac cgctttacgt
    10501 tctaacgttt tgtttaagaa tggtctttca gcaacatgtt taatattttc agttaactga
    10561 tgcaacgcaa ttttactatc tccaacaatt cctacattaa tattgaaacg atgtccgata
    10621 tttttaggat ttgtgtcaat ttgaatggct ttaatatttt tcttaggtaa gtaatccaca
    10681 tatggatagt ttgtaccaac cataattaat aaatccgctt cctgcattgt ttgataagat
    10741 gttttggtac cgattttacc taagttaccg atactatatg gatgatcatc cggcaagatt
    10801 gttttagctg gtaatgaatg aatgacagga attttagcca tttcaataaa ttcacgtagc
    10861 tcatctttcg catgtttcgc acctacacca attaacatga caggcttttt acttttatta
    10921 attagtttaa ccgctttttt gatgtcttta tattttggtg atactactgt tggtcttgat
    10981 gtatctactg gtttattcgt tgtatcttta attttttcag ttaataagtc gttaggacaa
    11041 ataacaacag ctacaccttt ttgttcatat gccgtacgaa ttgcttcgtt aacgatttca
    11101 aacacattgt cacctttttc aatttggtga ttataaacgg ctacatcttc acataatttt
    11161 tgtaaatttg tttcttggaa tgctttcgtt ccaagtgctg tactattcgt ttgtccagat
    11221 aatattaatt gcggtacatt atccattttg gcatcataca taccatttaa taaatgaatt
    11281 aaaccagggc caccgatact taatgccaca ccgattttac cagttaattt tgtgtaacca
    11341 gcagccgcta agcttgctac ttcttcatga cgtacatgat aaaatttaaa ttgatctctc
    11401 actgtacgta aactatcgac tactgcgtcg attgagtctc ctggaatacc atacaagtga
    11461 tctatatccc atgcttgtaa tgctttaact aatgcttcat ttgcttttat ttttgccatt
    11521 attactaata gcctcccttt ctgtctatca ttttaaatat ctaaattaat agaataataa
    11581 aattagaaca ggtattaaca ctgaactaat aactgcagtt aaaatcatcc caattgaact
    11641 aaatgcaccg gattcaatat ccatttctag tgctttagct gtgccaaatg catgtgacgc
    11701 attcccatac gttaatcctt tggcgataga agattcaaat cttccaaatc ttaataacat
    11761 cgaacctaaa atactaccga ttaaacccgt tgtgataata aaaagtacgg tcatcgtatc
    11821 tgtaccacct agttcatgtg acacttcgat acctactgcg gctgttatag atcggggcaa
    11881 taacgttaca atgacgtctt tagaatagcc aaatgcttta agtgttaaga acactaacat
    11941 gaaattcagc atgacgccag ttaatacact tgcaaaaatg atagagacat tgtctttaat
    12001 tttctctcta tttttataaa gtgggtacgc taaacatacg accgttgcgt ttaaaatatg
    12061 gttgatccaa ctgccacctt tcatataccc gttataacta attccaaaga taagtaagac
    12121 aaaaataatt cctaaagatg caattaatgc tggattcaaa aatgggttcg gatatttttg
    12181 ttgtaacctt ttagcgaaat aatataaaac gacagtcaac aaaatcatta ataaggcttg
    12241 cacgtaatca ttcataagcg tctacacctt tacgatgttt atgtttaaca gacatttttt
    12301 cagcaatata acctgaagat aatgcaacga tacatgttcc tatgataatg actgcgaaaa
    12361 agagtatata atttagcgta atttcggaag caacatccat aatacctaca actgacggta
    12421 tgaagaaaaa gaccatcgtc tttaataaaa agtttgcacc gtcttctacc caagttagcg
    12481 gtacaatctt aaattgtaat agtaaataaa ataaaaatag accaacaata ctgccggcta
    12541 agggaagatg aaaaatcttt tgaatttctg tgccaatata agtaatcaca atgatgattc
    12601 ctagttgtag tagtagtttg attattaatt ggactttgtg catggcgcca tccctttcta
    12661 aatatgtcta aattgttaca ataactatta taaagatggc gtacacaata taaaaatact
    12721 ctttttttat agtttgtatt actattttct atggaaacgc tctctaaatt tcacttcatt
    12781 ttcacaaaaa aacactcatt atttcaatga cccacgtaat gatgcgcctt aaataatgag
    12841 tgctttatta atatgttagc ctaaacggtc tttcaaaaat tctatccatt tcgttgtggc
    12901 atgacttaat tgtttatcct tcttccaaac aacacctaat tcccaatgta catgagcatc
    12961 ttcaatgcgt agcagcttca catctccatt tagttgctct gaaattgatg ttggtaaaat
    13021 actaatacct aattcattcg taactaaatc ttctataaca tgccattgtg aaatttgcgc
    13081 tacagtattc ggaacaaagc caacgttttt agcattttca ataattttat cattcaagta
    13141 aaagtcttta ttaaataaaa tgaagtcttc acctgctaaa tcttcgagtt taacagtttc
    13201 atattttgcg agtcgatgct ctctgctcac gataagtcgc aaatcttcct tatctagggt
    13261 agtataatcg aaaatatgat gatcgactgg caaagtggtc acgcctatat ctacttcatc
    13321 attgataatt tgctgttcaa ttgttttacc gccattttct atcaaattat atgtaacatt
    13381 tggatattgt tgatggaatg caccaagaat attgataaga atcttcatat tcatgactgc
    13441 cgacatgccc atgtttatat gtcctgtttc cagtccattc aagcgttcca tttcagatgg
    13501 taaataatca tacagtgcaa caatttcttt acttttctca taaaaaattt gacctgcatc
    13561 ggtaagaatt aaatgtcttt tacttctatc aaataagggc gtgcccattt cattttcaat
    13621 atctttaatt gctttactaa ttgtaggttg tgcaatatat aatgatttgg aagcattagt
    13681 catgcctcct tgcttaacga cttcaataaa atatttcata tgtttgatat ccactaacat
    13741 catcctaact atttcaacac gtttatttca ttatacacaa cttcttaaac attcaataaa
    13801 tttagattta taaaattgct ttataaaaag ttttatgtaa aatacacatt cttttatggt
    13861 attatattaa tggctcaagt atattaatcg aggtgaggta ttgagctttt acgaatttat
    13921 gcaaagtttt cttggtgatg acacaccatt aggtgaattg gttgattgga ttaatcaaga
    13981 tagcaatttc cctagagaag tgaagagcca tagtgaaata ttgtcatatt ttcgaacaaa
    14041 tccatgtcct gaaagcatct cagtacctgt aattaaacgg gcactatcag ttttcaacca
    14101 attcacaaat gtacaatgat acatgaatac atggagtcac atcatctcat gttagcgctc
    14161 tgattgcagg gcgctatttt taatgttatt gctacattta gtatattgtg attgctattt
    14221 tctttcagtt atagaaccat gtaccttttt tttaaatcat tttatatttt cccactaata
    14281 tccgctgtta atcaatcctg acatccttgt atcactatga caattaattg ttaaatacat
    14341 gaatttctac attttatgaa aaaatccatt tttattacaa ttcaacactt tatatgacaa
    14401 cttcattaca gttactttta ttgttgattg cttacattgt tttctaaaaa aaatttgtta
    14461 tcataattaa cgttgaataa agaaaaaaat taacttggga gataaaaatg gaatataaaa
    14521 agatactaat tcgtttatta attgcttttg cagtactttt ctcagcagat ttcacttatc
    14581 aatccgtcga acaaacgcat caatcgcatg ccgcagttaa ttattatagt aaaaaccaat
    14641 gtacatggtg ggcatttaaa cgtcgcgcac aagtcggtaa acctgtttct aatagatggg
    14701 gcaatgctaa aaattggtat tacaatgcac gtaaatcaaa atatgcgact ggtcgtacac
    14761 caagaaaatt tgctgtcatg caatcaactg caggatatta tggacatgtc gcagttgttg
    14821 aacaagtata taaaaacggt agtattaaag tttcagaata caacttttat cgcccattaa
    14881 aatacaatac acgtgtacta agcaaaaagg cagcacgtaa ctttaactat atttactaat
    14941 caaaaaactt ctatcacgaa cgcttcaatt tcctgtatgc gtgtgataga agtttttatt
    15001 ttatgaaatt atattattac ttctacaaat ttcaaattgc cgtaattgaa cgtatatttc
    15061 ttcttcaact attatttcat ctttagcata atctatatat aaaattttat gctatt
//
LOCUS       DAMQYT010000048        14847 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMQYT010000048 DAMQYT010000000
VERSION     DAMQYT010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14847
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.048"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            414..878
                     /gene="accB"
                     /locus_tag="PGA33_001133"
     CDS             414..878
                     /gene="accB"
                     /locus_tag="PGA33_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDG6472958.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQAQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            878..2233
                     /gene="accC"
                     /locus_tag="PGA33_001134"
     CDS             878..2233
                     /gene="accC"
                     /locus_tag="PGA33_001134"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDG6472959.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            2248..2610
                     /locus_tag="PGA33_001135"
     CDS             2248..2610
                     /locus_tag="PGA33_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDG6472960.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            2670..3059
                     /gene="nusB"
                     /locus_tag="PGA33_001136"
     CDS             2670..3059
                     /gene="nusB"
                     /locus_tag="PGA33_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDG6472961.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKGHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            3076..4413
                     /locus_tag="PGA33_001137"
     CDS             3076..4413
                     /locus_tag="PGA33_001137"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDG6472962.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            4406..4636
                     /locus_tag="PGA33_001138"
     CDS             4406..4636
                     /locus_tag="PGA33_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDG6472963.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            4614..5495
                     /locus_tag="PGA33_001139"
     CDS             4614..5495
                     /locus_tag="PGA33_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDG6472964.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDTTKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
     gene            5927..6379
                     /gene="argR"
                     /locus_tag="PGA33_001140"
     CDS             5927..6379
                     /gene="argR"
                     /locus_tag="PGA33_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDG6472965.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            6395..8074
                     /gene="recN"
                     /locus_tag="PGA33_001141"
     CDS             6395..8074
                     /gene="recN"
                     /locus_tag="PGA33_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDG6472966.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELISDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            8225..9646
                     /gene="lpdA"
                     /locus_tag="PGA33_001142"
     CDS             8225..9646
                     /gene="lpdA"
                     /locus_tag="PGA33_001142"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDG6472967.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDTNEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            9662..10654
                     /locus_tag="PGA33_001143"
     CDS             9662..10654
                     /locus_tag="PGA33_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDG6472968.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEERETLKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            10654..11637
                     /locus_tag="PGA33_001144"
     CDS             10654..11637
                     /locus_tag="PGA33_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDG6472969.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGL
                     QQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            11650..12924
                     /locus_tag="PGA33_001145"
     CDS             11650..12924
                     /locus_tag="PGA33_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDG6472970.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTSTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTAEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIIKKPVVINDMIAIRNMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            13275..13712
                     /locus_tag="PGA33_001146"
     CDS             13275..13712
                     /locus_tag="PGA33_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDG6472971.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            13726..14706
                     /locus_tag="PGA33_001147"
     CDS             13726..14706
                     /locus_tag="PGA33_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDG6472972.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEVLLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
BASE COUNT         5553 a         2029 c         2814 g         4451 t
ORIGIN      
        1 aataagtctc ataaagctat tgcctaaaat gattataggt tatatgctga tatgaggctt
       61 tttattttta aaataatttt taaaatataa aacgaatcgt ctaattggta gtgaaaaaac
      121 atagacatga gtagaggtat atatctacat atcatattgt tgaggataaa acataatagc
      181 taataaaaat aacattgata tgtttaactg aatgcataaa atttttaatc aagagttaag
      241 atgagtcaat gttaagtaat aaatacacaa gtgtttttaa tttaaaatgt ggttttatta
      301 taactttggg atagacttat aatgtcgcca gcaatatgaa catgcttgaa ttgaagagtt
      361 gtctcaagta aaatagacgg gtagatgaaa acaaactgaa ggagtcagta ataatgaact
      421 ttaaagaaat caaagaatta attgaaattc tggataaatc aactttaacg gaaatcaata
      481 ttgaagatac taaaggcaaa gtgacgctta agaaagaaaa agaaactgag attatcacgc
      541 cacaaatctc acaaatgcca gttgaagctg cggcaatgcc tatgcctcaa gcacaatcaa
      601 ctgatagcaa taaaactgaa gctccaaagc caacttcaga taatcacaaa acaattaatg
      661 cacctatggt aggtacattt tacaaatcgc catctccaga cgaagaagca tatgtgcaag
      721 ttggggacac tgtttcaaat gaaacaacag tgtgtatttt agaggcaatg aaactattta
      781 atgaaattca agcagaaatt tcaggtgaaa ttgttgaaat cttagtagaa gacggacaaa
      841 tggtagagta tggccaaccg ttatttaagg tgaaataatg aaaaaggttt taattgcaaa
      901 ccgcggtgaa atcgcagtta ggattattcg cgcttgtcgt gatttaggca tccaaactgt
      961 tgcaatctat tctgaagggg ataaagatgc gctacatact caaattgctg atgaagcata
     1021 ttgcgtaggt cccactttgt ctaaagattc atatttaaat attccgaaca tcttatctat
     1081 tgcaacttct acaggttgtg atggcgttca tccgggttat ggctttttag ctgaaaatgc
     1141 tgattttgca gaattatgcg aagcatgcca attgaagttc attggaccaa gttatcaatc
     1201 tatccaaaaa atgggtatca aagatgttgc taaggcagaa atgatcaaag ccaatgttcc
     1261 agttgttcct ggtagtgacg gtttaatgaa agacgtctca gaagctaaga aaatcgccaa
     1321 aaaaattggc tatccggtca tcattaaagc tactgctggc ggtggcggaa aaggtatccg
     1381 tgttgctcgt gatgaaaaag aacttgaaac tggcttccga atgacagaac aagaagctca
     1441 aactgcattt ggtaatggtg gactttatat ggagaaattc atcgaaaact tccgccatat
     1501 tgaaatccaa attgttgggg acagctatgg taatgtaatt catttaggag aacgtgattg
     1561 tacaattcaa agacgtatgc agaaattagt ggaagaagca ccttccccaa ttttagatga
     1621 tgaaacacgt cgtgaaatgg gaaatgccgc agttcgtgca gcgaaagctg taaattatga
     1681 aaatgcggga acaattgagt ttatatatga tttaaatgat aataaatttt attttatgga
     1741 aatgaataca cgtattcaag tagaacatcc tgtaactgaa atggtaacag gaattgattt
     1801 agttaaatta caattacaag ttgctatggg tgacgtgtta ccgtataaac aagaagatat
     1861 taaattaaca ggacacgcaa ttgaatttag aattaatgct gaaaatcctt acaagaactt
     1921 tatgccatca ccaggtaaaa ttgagcaata tcttgcacca ggtggatatg gtgttcgaat
     1981 agagtcagca tgttatacta attatacgat accgccatat tatgattcga tggtagcgaa
     2041 attaatcata catgaaccga cacgagatga agcgattatg gctggcattc gtgcactaag
     2101 tgaatttgtg gttcttggta ttgatacaac tattccattc catattaaat tattgaataa
     2161 cgatatattt agaagcggta aatttaatac aaacttttta gagcaaaata gcattatgaa
     2221 tgatgaaggt taataggagg tcaatccatg gtcaaagtaa ctgattattc aaattcaaaa
     2281 ttaggtaaag tagaaatagc gccagaagtg ctatctgtta ttgcaagtat agctacttcg
     2341 gaagtcgaag gcatcactgg ccattttgct gaattaaaag aaacaaattt agaaaaagtt
     2401 agtcgtaaaa atttaagccg tgatttaaaa atcgagagta aagaagatgg catatatata
     2461 gatgtatatt gtgcattaaa acatggtgtt aatatttcaa aaactgcaaa caaaattcaa
     2521 acgtcaattt ttaattcaat ttctaatatg acagcgatag aacctaagca aattaatatt
     2581 cacattacac aaatcgttat tgaaaagtaa tgtcatacct aattcagtaa ttaaataaag
     2641 aaaaatacaa acgtttgaag gagttaaaaa tgagtcgtaa agaatcccga gtgcaagctt
     2701 ttcaaacttt atttcaatta gaaatgaagg acagtgattt aacgataaat gaagcgataa
     2761 gctttattaa agacgataat ccagatttag acttcgaatt tattcattgg ctagtttctg
     2821 gcgttaaagg tcacgaacct gtattagacg agacaattag tccttattta aaagattgga
     2881 ctattgcacg tttattaaaa acggatcgta ttattttaag aatggcaaca tatgaaatat
     2941 tacacagtga tacacctgct aaagtcgtaa tgaatgaagc agttgaatta acaaaacaat
     3001 tcagtgatga tgatcattat aaatttataa atggtgtatt gagtaatata aaaaaataaa
     3061 attgagtgat gttatatgtc agattattta agtgtttcag ctttaacgaa atatattaaa
     3121 tataaatttg atcaagatcc acatctccaa tctgtattga ttaaaggtga gctttcaaat
     3181 ttcaaaaagc attcaagtgg tcatttatac tttaatgtga aagataaaga aagcgttata
     3241 agtgccatga tgttcaaagg tagtgcttct aaattaaact tcgaacctaa agaaggagat
     3301 gaagtcttat tagaagcacg tgtttctgtt tttgaacgtc gtggaaacta tcaaatttat
     3361 gtaaataaaa tgcaattaga tggtataggg aacttatatc aaaaattaga agcattaaag
     3421 aaaaaattaa ctgaagaagg ttgttttgat aaagcgaata agaaatcaat acctaagttt
     3481 cctaaaaaaa ttgcagtttt aacagcgagt acaggtgccg caattcgaga tatccattca
     3541 acgataaata gtcgttttcc attagctgaa caaatacaaa ttagtacttt ggttcaaggt
     3601 gaaaaagcaa aagacgacat tattgaaaaa attgaatatg cagatagttt aggtgtagat
     3661 accattattg taggtcgagg cggtggatcc atagaagatt tatggaattt caacgaagaa
     3721 gctgtcgtcc gtgcaatata taattgtaag acaccgatta tatcagcagt tggtcatgaa
     3781 acagacttta cattaagtga ttttgctgca gacatcagag ctgcgactcc aactcaagct
     3841 gctgttattg caacaccaga ccaatatgaa ttgctgcaac aaatacagca atatcagttt
     3901 acattgactc gtttcatcaa gaaacattta gaacaacaac gtaaacatgt tgaacatttg
     3961 tcatcgtatt acaagtttaa acaaccaact ttattgtatg atcaacagat acaacgtcga
     4021 gatgatttag aaaagagact gaaacaacaa attcaggcaa cttttgaaca acagagacat
     4081 cgattaatgc tgttgcaaca acgttataat ttaaaagcat tactaagtag tgttaatcaa
     4141 gaacaacaaa ataatcttca attgacaaac caacttgtta aattattgaa ttctaagata
     4201 ttaagttata aaaatgattt gaaaaataaa gtagaaaact taaacaattt aagtccaact
     4261 aatacaatgt tgcgtggata tgcaattgtt aataaaaaag acgaagtcat tacgagtact
     4321 aaagatttaa cagaaaatga tcaattgaca ttaacaatga aagatggctt agtagatgca
     4381 aaagttacga aagtaaggtg taataatgac taaagaaacg caaagttttg aagaaatgat
     4441 gcaagaatta gagcaaattg ttcaaaaatt agataatgaa acagtatctt tagaggaatc
     4501 attagattta tatcaacgtg gtatgaaact atcagcagct tgtgacacaa ctttaaaaaa
     4561 tgccgaaaaa aaggtgaatg acttaataaa agaagaagct gaggatgtaa aaaatgacga
     4621 atctaccgat gaataaatta atagatgaag tcaataatga attatcggtt gcgataaata
     4681 aatcagtaat ggatactcag ctagaagaaa gtatgttgta ttcattaaat gctggaggta
     4741 aacgcatccg accagttctg ttattactca ctttagattc actaaatacc gagtatgagt
     4801 taggtatgaa gagcgcaatt gcactagaaa tgattcatac atattcactt attcatgatg
     4861 acctaccagc gatggataat gatgattatc gacgaggaaa attaacaaat cataaagtat
     4921 atggtgagtg gactgcgata ttagcaggtg atgctttatt aactaaagca tttgaactta
     4981 tttcaagtga tgatagatta actgatgaag taaaaataaa agttctacaa cggctgtcaa
     5041 tagcaagtgg tcatgttgga atggtcggcg gtcaaatgtt agatatgcaa agcgaaggcc
     5101 aaccaattga tcttgaaact ttggaaatga tacacaaaac aaaaacagga gcattattaa
     5161 cttttgcggt tatgagtgca gcagatatcg ctaatgtcga tgatacaact aaagaacatt
     5221 tagaaagtta tagttatcat ttaggtatga tgttccagat taaagatgat ttattagact
     5281 gctatggtga tgaagcaaag ttaggtaaaa aagtgggcag cgatcttgaa aataataaaa
     5341 gtacgtacgt gagtttatta gggaaagatg gcgcagaaga taaattgact tatcatagag
     5401 acgcagcagt ggatgaacta acgcaaattg atgaacaatt caatacaaaa cacttattag
     5461 aaatcgttga tttattttat agcagggatc actaaaagca aatatatcga ttagcaacaa
     5521 ttgtaaagtt tgaaatgtct caaaaggcat attcaactga cgttgtttgt taatcgattt
     5581 ttctcatgtc gaaacttaaa attatgttga agactttcat ttaaaaaata gaattttcaa
     5641 tttctatacg acaatattcg tatctgtgat atcaatgtga ttttaattaa atatgttcaa
     5701 acaattgacg cttattaaat ctaataatta gcaagtttat tacacatata ttcattttta
     5761 tacatatttt aagcaattgt tatgcgtaat atgaaaaata tttataaata gataaccaag
     5821 atagataaac catgtttacc ctttttacgt aaaggtgttt agtagtcgta aatcagtgca
     5881 tgtgttaaaa taaatgtata aatattcgta atcagaggtg tttactgtgc ccaaaaaatc
     5941 ggttaggcat ataaaaatta gagaaattat ttcaaatgaa cagatagaga cacaagatga
     6001 attagttaaa cgattaaacg attatgattt aaatgtcact caagcaactg tttctcgtga
     6061 tattaaagaa ctacaactta ttaaagtacc tataccttca ggtcaatatg tttatagttt
     6121 accaaatgat agaaaattcc atcctttaga aaaattggga cgttatttaa tggattcctt
     6181 tgttaatata gatggtactg ataatttact tgttctaaaa acattacctg gtaatgcaca
     6241 atctattgga gctatattag accaaatcaa ttgggaagaa gtactaggca caatttgtgg
     6301 tgatgatact tgtttaatta tttgtcgaag caaagaggca agtgatgaaa tcaagtcaag
     6361 aattttcaat ttgttataag gatgcgatga gtttatgtta caaaccttat caatcaagca
     6421 atttgctatt attgaagaat tagaaattca attttctgat ggtttaactg ttttaagtgg
     6481 tgaaacgggt tcaggtaaat caattattat agatgcaatt ggacaattaa ttggaatgag
     6541 agcttcatct gattttgtaa gacatggtga aaaaaaggct gtcatcgaag gtatatttga
     6601 tattgacgag agtaaagatg ctatacatat attgaaaaat atggatattg atgtagatga
     6661 agatttttta ttagttaaac gggaaatttt cagctctggt aaaagtcttt gtaaaattaa
     6721 taatcaaaca gttactttac aagatttacg aaaagttatg caagaattat tagatattca
     6781 tggccaacat gaaacgcagt ctttgttgaa acaaaaatac catttaacat tattggataa
     6841 ttacgcagaa tcacgttatc aagatttgtt ggataaatat caccaaactt ttcaaaatta
     6901 taaagccaaa aagcaagagt tagaagattt agaatcagca gaccaagcat tgctacagcg
     6961 tttagattta atgaaattcc aattagaaga attgtctgaa gcacatctaa aagaaggcga
     7021 aatagagcaa cttgaaatag atattaaacg cattcaaaac tctgaaaaat taagcttagc
     7081 gcttaataac gcacatatga cattaacgga tgaaaatgct atcactgatc gtttatatga
     7141 actaagcaac catttgttaa caataaacga tatagttcca aacaaatatg acaaattaaa
     7201 agaagatatt gatcaatttt actacatttt agaagatgca aaacatgagt tatatgatga
     7261 aatggctaat acagaattcg acgaacaagt gttaaacgag tatgaatctc gtatgaattt
     7321 gctgaataat ttaaaacgta aatatggaaa agatatttca gaattaatcg catatcaaga
     7381 aaagcttaat aatgaaatca ataaaattga aaactatgaa caaagtacat cgcagttacg
     7441 agaagaaata aatgcattgt ataatcaagt tatagaggtt ggacaagcgt tatcaaagca
     7501 gcgtcgtata gtcgctaggg agttaagaga ccacattgta tcggaaattc aaaacttaca
     7561 aatgaaagac gcaaatcttg agatttcatt taaaaaatta gaagaaccga atattgatgg
     7621 aatcgaattt gtagaatttt taatcagtcc aaataaaggg gaaccattaa aaagtttaaa
     7681 taaaattgcg tcaggtggag aactttctag aattatgtta gcgttaaaaa gtatttttgt
     7741 taaatcgaga ggtcaaactg caattttatt cgatgaggtt gactcaggtg tatctggaca
     7801 agctgcacaa aaaatggcag agaaaatgcg agatatagca gaatatatac aagttatttg
     7861 tatctctcac ttaccacaag ttgcatcgat gagcgaccat catttattaa tttcgaaatc
     7921 atcaaaagat gatcgtacaa caacacaagt tcaagagtta attagcgatg ataaagtaga
     7981 tgaaattgca cgaatgattt caggtgcaag tgttactgat ttaactcgag aaaatgcaag
     8041 agaaatgata caacacaatc aaagacgtag ataatttcca aagtgtgttg aatactcatt
     8101 tatggattta tttatgtgca ttcaaactaa atagacaatc atgtaaaata ggtaattaat
     8161 ggacatgaaa actatgtatt aataaatttt caatggatat atatcattag ttaggagtaa
     8221 gaacatgtca gagaaacaat atgatttagt cgttctcggt ggaggtactg caggatatgt
     8281 tgctgcgata cgtgcgtctc aattaggtaa aaaagtggca atagtggaac gacaactatt
     8341 aggaggtact tgtctccata aaggttgtat tcctactaaa tcgctgctta aatctgctga
     8401 agtatttcaa acagtaaaac aggcagcaat gtttggcgtt gatgtcaaag atgctaatgt
     8461 taattttgaa aatatgttgg cacgaaaaga agacattatt aatcaaatgt atcaaggtgt
     8521 aaagcattta atgcaacaca atcacattga catttataat ggcacgggac gtattctagg
     8581 tacatctata ttttcacctc aaagtggcac aatttctgtc gaatatgaag atggtgaatc
     8641 agacttatta cccaaccaat ttgtattaat tgcaactggt tcttcgcctg cagagttgcc
     8701 attcttatca tttgatcatg ataaaatttt atctagcgat gatatattat cattaaagac
     8761 gttaccatca agtattggta ttattggtgg tggtgttatc ggaatggaat ttgcatcatt
     8821 gatgatagat ttaggtgttg acgtaacagt tatagaagca ggtgaaagaa ttttaccaac
     8881 tgaaagtaaa caagcttcac aactattaaa aaaatcattg tcagcacgtg gtgttaaatt
     8941 ctatgaggga ataaaacttt ctgaaaatga tataaatgtt aacgaagatg gtgttacgtt
     9001 tgaaatttcc tcagacataa ttaaagtaga taaagtgttg ctatcgattg gtagaaaacc
     9061 taacacatca gatattggtt taaacaacac taaaataaaa ctttcaacat caggacatat
     9121 tttaacgaac gaatttcaac aaactgaaga taaacatatt tatgcagctg gtgattgtat
     9181 aggaaaatta caattggcac atgttggatc aaaagaaggt gttgtggctg ttgatcatat
     9241 gtttgagggg aacccaatcc cagtaaacta taacatgatg cctaagtgta tttattcaca
     9301 acctgaaatt gcttctatcg gtttaaacat tgaacaagca aaggcagagg gaatgaaagt
     9361 taaaagtttt aaagtaccat ttaaagcaat tggtaaagca gtgattgata gccatgacac
     9421 aaacgaaggg tatagcgaaa tggtgattga tcaatcaact gaagaaattg tgggtattaa
     9481 tatgattggt ccacatgtaa cagaattgat taatgaggca tcactgttac agttcatgaa
     9541 tggctcggca ttagaattag gactaacaac acacgcacat ccttccatct ctgaagtgtt
     9601 gatggaatta ggattgaaag cagaaagtag agctattcac gtataaatag gaggatttaa
     9661 catgattgat tataaatcat taggccttag cgaagaagac ctaaaagtaa tatataaatg
     9721 gatggattta ggtagaaaaa tagatgaaag actgtggtta ttgaaccgtg caggtaaaat
     9781 tccatttgtt gtaagtggtc aaggacaaga agcaactcag attgggatgg catacgcatt
     9841 agaagaaggt gatattacag cgccttatta tagagatcta gctttcgtta cttatatggg
     9901 tatctcagcg tatgatacgt ttttatcagc gtttggaaag aaagacgatg ttaactctgg
     9961 tggaaaacaa atgccatcac actttagtag tagagctaag aatattttgt ctcaaagttc
    10021 accggtagca actcaaatac cccatgctgt tggtgctgct ttagctttaa aaatggatgg
    10081 taagaagaaa attgcaacag caactgttgg tgaaggtagc tcaaaccaag gtgacttcca
    10141 tgaaggttta aacttcgcgg gtgtacacaa acttcctttt gtatgtgtca ttataaataa
    10201 taaatatgca atttcagtac ctgattcatt acaatacgca gctgaaaaat tatcggatag
    10261 agcacttggt tacggtatac atggtgaaca agtagatggt aatgatccat tagcaatgta
    10321 taaagcgatg aaagaagcaa gagatcgagc tatttctggt caaggttcaa ctttgattga
    10381 agcagtaaca agtcgtatga cagctcattc atcagatgat gacgaccaat atcgtacgaa
    10441 agaagagcgt gaaacgctta aaaaagcaga ctgcaatgaa aagttcaaaa aagagttgct
    10501 ttcagctggc attatcgatg atgcttggtt ggcagaaata gaagcggagc ataaagacat
    10561 catcaataaa gcgacaaagg ctgctgaaga cgcgccatat ccaagtgtcg aagaagccta
    10621 tgcatttgtg tacgaagaag ggagtcttaa ctaatggcta aattatctta tttagaggcg
    10681 atacgccagg cacaagactt agcattacaa caaaataaag atgtgtttat tcttggtgaa
    10741 gatgttggta gaaaaggtgg cgtatttggt actactcaag gactgcaaca aaaatatggt
    10801 gaagacagag ttatcgatac gccattagct gagtcaaata ttgttggaac tgccattggt
    10861 gcagctatgg taggtaaaag accaattgca gaaattcaat ttgcagattt tattttacct
    10921 gcgacaaatc aaattattag tgaagctgcg aaaatgcgtt atcgttcaaa taatgattgg
    10981 caatgcccat taacgattcg tgcaccattt ggtggcggtg ttcacggagg actatatcat
    11041 tcacaaagta ttgaaagtat atttgcttca tcgccaggtt taacaattgt tattccttcc
    11101 acaccgtatg atgctaaagg gcttttatta tcatctatcg aatcaaatga tccagtattg
    11161 tactttgagc ataaaaaagc ttatcgtttc ttaaaggaag aagtgccaga agaatattac
    11221 actgtacctt taggaaaagc agatgtgaaa cgtgaaggtg aagatttaac agtattttgt
    11281 tatggattaa tggtcaatta ttgtttacaa gcagcggata ttttagctgc agacggtatc
    11341 aatgttgaag tcgttgattt acgaactgtt tatccattag ataaagaaac aattattgat
    11401 cgtgctaaac atacaggtaa agttttactt gtaactgaag ataatttaga aggtagtatt
    11461 atgtcagaag tgtcagcgat tattgcagag cattgcttgt tcgatttaga tgcaccaatc
    11521 atgcgtttag ctgctccaga tgtaccatct atgccatttt ctcctgtatt agaaaatgaa
    11581 attatgatga atccagaaaa aatcttaaat aaaatgcgtg aattagcaga attctaggga
    11641 gggaaagtca tggaaataac aatgcctaag ttaggtgaga gtgttcatga aggcaccatt
    11701 gaacaatggt tagtttctgt tggtgatcat attgatgaat atgaaccatt atgtgaagtt
    11761 attacagata aagtgacagc tgaagtccct tccacgatat caggaacaat tacagaaatt
    11821 ttagttgaag cggggcagac agtagctatt gatacaatta tctgtaaaat tgaaactgct
    11881 gatgaaaaga caaatgaaac aactgaagag atacaagcaa aagtggatga gcatactcag
    11941 aaatctacta aaaaagctag tgcaacagtg gaacagacat ctactgctaa acaaaatcaa
    12001 ccacgtaata atggtcgctt ttcacctgtt gtatttaaac tcgcttcaga gcatgacatt
    12061 gatttatcac aagttgtagg tagtggattt gaaggtcgtg taactaagaa ggatataatg
    12121 tcagttattg aaaatggtgg taccacagct caatctgaca aacaagttca aacaaaatca
    12181 acatcagtag atacatcaag taaccaatca tctgaagaca atagtgaaaa cagcacaata
    12241 ccagtaaatg gtgtgcgtaa agcaattgcg caaaatatgg ttaatagtgt aacagagatt
    12301 ccacatgcat ggatgatgat tgaagtagat gctacaaatc ttgtgaatac gagaaatcat
    12361 tataaaaaca gctttaaaaa taaagaagga tataatctaa cgttctttgc tttctttgta
    12421 aaagctgtag cagatgcttt aaaagcatat cctttattaa atagtagctg gcaaggaaat
    12481 gaaattgtct tacataaaga cattaatatt tcaattgctg ttgctgatga aaataaatta
    12541 tacgtacctg tgattaagca tgcagacgaa aagtcaatca aaggtatagc tagagaaatt
    12601 aatactttag caacgaaagc gcgtaataag caattgacag ctgaagatat gcagggcggt
    12661 acatttacgg taaataatac tggtacattt ggttcagtat catcaatggg tattataaat
    12721 catccacaag cagcgatttt acaagtagaa tcaatcatta aaaagccagt agtaattaat
    12781 gatatgattg caattcgtaa catggttaat ttatgtattt caattgatca tcgtatttta
    12841 gatggtttac aaacaggtaa atttatgaat catattaaac agcgtatcga acagtatact
    12901 ttagaaaata caaatatata ttagtgataa catagatgca tctatcgaca acttgtttta
    12961 tcttgttctt gtcgatggat gtattatttt ttggcactaa aatatgtgca atatattcaa
    13021 aaaagataaa gaacaataat caacatggtt gaatgcattt ttgcagtaaa gtcaaaataa
    13081 gacatcatac ttgaaacata ttaatgaaaa catgtgaaca aattagttac catgatttta
    13141 agcacaataa tgtttggtat attgttaaaa ttgtgtctaa atataggtgt gattcagatt
    13201 agtttattga acaatatgtt attaattagt agaatgagga tagtttaaat ataaagggat
    13261 aggtgattga acttatggac atgaatttcg atttatacat gaacggtgtt gtagaacaag
    13321 caaggaatga aattgaatct gcgggatatg agcaattaac tactgcagaa gatgttgaca
    13381 aagttcttaa acaagatggt acaacactag ttatgatcaa ttctgtatgt ggttgtgcag
    13441 gtggtatcgc aagaccagca gcatcacatg ctttacatta tgacgtatta cctgatcgtc
    13501 tagtgacagt atttgctgga caagataaag aagcgacaca aagagcgcgt gaatacttcg
    13561 aaggttatgc gccttcaagt ccgtcatttg cattagtaaa agatggaaag attacagaaa
    13621 tgattgaaag acatcaaatc gaaggtcatg atgtgatgaa cgtaattaat caattacaaa
    13681 cattattcaa taaatattgt gaagaaagat aagaggcgct aacccatgtt aaagttaaat
    13741 ccttacaaga ttggatttag aacaataaaa acagcagtgg gtatgacttt aggtgtaatt
    13801 attagtaagc tgttaggttt agataattat gcttcaagcg ccatattagt cgtattatgt
    13861 attaaacata caaaagtaca ttcgctacaa gcgattattt caagattagt atcatgtttt
    13921 ttagtattgt ttttaggttc agcaatattt agtttattag gtcagagtcc aattgtactc
    13981 ggtattatcg tattgttatt tataccatta actgtcgtat taaaagtaca agaaggtgtc
    14041 attacgagtt gcgttatatt acttcatgtt tttaatgcaa aatcaattga tgcacattta
    14101 attgttaatg aaacattatt actgttaatt ggactaagca ttgcatttac aatgaattta
    14161 atgatgccaa gtttagacaa acaactagac gaatacaaat gtaaaattga gcaacaaatt
    14221 gctgatattt ttagtaaata tagttatatt tgtgaaaaat atgaagatac cattgcgatt
    14281 gaatttgaag tgttactttt aaatattaaa aaggcgaagt ctatcgcgtt ccgagatgtt
    14341 aaaaatcatt ttgttagaaa cgaaaattca tactatcatt attttgatat gcgagaagag
    14401 caagtggaat tgttaatgag aatgaaaccg ctcatcgaaa gtatctgtca taaagatccg
    14461 cttttaaatg atgttgcgtc actgctgaaa gaaattggtc taaatgtgaa tagtaatgac
    14521 tatacagcaa tgcgtttaca caatttgtat gagacgcgtt tgaaattgga tgaactgcca
    14581 ttgccaacaa cacacaaagc gcttaactcg agagcaagta ttattcaaat attaaatgaa
    14641 ttagaagaat atttacaaat caaatcgcaa tttggttcat tgaaaatgca tagtgaagta
    14701 gcttaaaaaa taatccatcc gtatgagcca tgtttgaaag taatcgttgc aatacgataa
    14761 cttattaata catgcgttta taatggatgg atttttaatt gtatgatttt gtataggtag
    14821 ttgagatata gtttataaaa ttggaat
//
LOCUS       DAMQYT010000049        14751 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMQYT010000049 DAMQYT010000000
VERSION     DAMQYT010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14751
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.049"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(174..1172)
                     /gene="sirC"
                     /locus_tag="PGA33_001148"
     CDS             complement(174..1172)
                     /gene="sirC"
                     /locus_tag="PGA33_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HDG6472973.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1169..2164)
                     /gene="sirB"
                     /locus_tag="PGA33_001149"
     CDS             complement(1169..2164)
                     /gene="sirB"
                     /locus_tag="PGA33_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HDG6472974.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2180..3172)
                     /gene="sirA"
                     /locus_tag="PGA33_001150"
     CDS             complement(2180..3172)
                     /gene="sirA"
                     /locus_tag="PGA33_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HDG6472975.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3403..4383
                     /gene="sbnA"
                     /locus_tag="PGA33_001151"
     CDS             3403..4383
                     /gene="sbnA"
                     /locus_tag="PGA33_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HDG6472976.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4380..5390
                     /gene="sbnB"
                     /locus_tag="PGA33_001152"
     CDS             4380..5390
                     /gene="sbnB"
                     /locus_tag="PGA33_001152"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HDG6472977.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5411..7165
                     /gene="sbnC"
                     /locus_tag="PGA33_001153"
     CDS             5411..7165
                     /gene="sbnC"
                     /locus_tag="PGA33_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HDG6472978.1"
                     /translation="MQNHTAVNTAQAIILRDLVDALLFEDIAGIVSNSEITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRVSKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTVPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7158..8414
                     /gene="sbnD"
                     /locus_tag="PGA33_001154"
     CDS             7158..8414
                     /gene="sbnD"
                     /locus_tag="PGA33_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HDG6472979.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            8404..10140
                     /gene="sbnE"
                     /locus_tag="PGA33_001155"
     CDS             8404..10140
                     /gene="sbnE"
                     /locus_tag="PGA33_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HDG6472980.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPMSKLSQVIEQSGLAPE
                     AWLECYLNRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            10049..11899
                     /gene="sbnF"
                     /locus_tag="PGA33_001156"
     CDS             10049..11899
                     /gene="sbnF"
                     /locus_tag="PGA33_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HDG6472981.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDMYLKDELKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEELQWQWVKGIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            11874..12650
                     /gene="sbnG"
                     /locus_tag="PGA33_001157"
     CDS             11874..12650
                     /gene="sbnG"
                     /locus_tag="PGA33_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HDG6472982.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDEG"
     gene            12650..13852
                     /gene="sbnH"
                     /locus_tag="PGA33_001158"
     CDS             12650..13852
                     /gene="sbnH"
                     /locus_tag="PGA33_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HDG6472983.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERKILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPKIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHRF
                     PLKHINLGGGIGVNYADLTNQFEWDNFVERFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13856..14620
                     /gene="sbnI"
                     /locus_tag="PGA33_001159"
     CDS             13856..14620
                     /gene="sbnI"
                     /locus_tag="PGA33_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HDG6472984.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
BASE COUNT         4894 a         2426 c         2953 g         4478 t
ORIGIN      
        1 attatagaat atgagtcggt gttatttgta tataaagtat taatatgtaa ttaagaaagt
       61 cgacgattgt taacaataac aacaactata tgtataaaaa aacctcgcaa cggttagtta
      121 actgaatcat ttagctaact tcgttgtgag gtcattttgt tttaataata tcgttataac
      181 tttttcacgg ttaataataa gtatatgaag aatggggcac caaaagcagc aataaataca
      241 cctgctggca cttctttagg caagaataag gtacgcccaa ttaagtctgc aataacaatt
      301 gatatggcac caatcattgc tgacattagt aactttttag cataacttcc gcgaacgatt
      361 gttttcgcga tatgtggtgc gattaaaccg acaaacccaa tgttacctac taaactgatt
      421 gccatagata cgagtatagt agaagtgatt aattggatta gtttcatacg ttgtacatgt
      481 aagcctaagc caatcgctac agggtcatca agtatagata ttttcatttt tggtataaca
      541 agaaataaca acggcacaac agctaaaata accataccca aaatgattgt atctttaaac
      601 gtagcaccgt aaagacttcc gactagccat gtataagctt tggcagcaga taattgcttc
      661 gttgtaatga gtaatccttg gacaagcgca ataaacaacg tttgcatcga aataccgatg
      721 attatgagtg ttgtcgggcg tatttgtcct ttcgtttgaa acactaatag tatcatcatt
      781 gcaactgcgc cacctaatac tgcaaatagt ggaagtaaat gtattgttaa atggctgaaa
      841 aatgcaataa agacaacagc acttaagcta gcaccacctg tgataccgat aatatcaggt
      901 gaggcaattg gattttttaa tacattttgc aacattaaac cactcattcc tagtgcggca
      961 cctgctaaaa tcgcaagtgt aatgcgaggt aagcgtaata cttctaaagt gaattgatcc
     1021 atactgtcat ttggatttat aaagtacatc agtacgcgtt gtaatggtat aaagcttgaa
     1081 ccaatcatca tacttaccac tgaaacgatg gctaaaaaga ttaacgcgaa gatgagatgg
     1141 taattgtctt ttttattaat cttttcggtc ataagcgttg acgtcctttc ttcataatat
     1201 agattaagac aatagcgcca atgacagcgg taacgacacc gataggcaac tctagtggct
     1261 taattattat acgagcaaca atgtctgaaa tgatcattag gattgctcca gctaatgcag
     1321 taaaaggaat taaatactta tagtttggtg gtaataatcg tttgctaata ttcggtacga
     1381 taagacccac aaagacgatt gatccagcta cggctaccga aataccggct aacatactga
     1441 tgagcataat aatcatccat ttgattaatt ttatgttttg accgaggccg gttgcaatgt
     1501 cgtcacttgt catcaagatg ttgatgtgtg cagccatgct aaatgcaatt aaaataagta
     1561 tcaatacaag cggaataatc catgggatat cccaaatatt acgtaatgaa acggagccac
     1621 ttaaccaaaa taataggcct tgtaagtctg tttcgttcat aataagtatg ccttgagtaa
     1681 aggctgtaaa tagcatcgca atcgcagcac ctgccaaaat gacacggtga ggtgagaata
     1741 gtgtttgtct aaacatacct agtgcaacaa ctaatacagt aacaacaata gcccccaaaa
     1801 atgcaataac tacaatcatt ttaaaagatt gaatttggat aaatgtaata ctaaaaatga
     1861 caaaaaatac tgcgcctgca ttgacaccga aaagccctgg tgaggctatt gggtttcgtg
     1921 taagtgcttg catcaacaaa cctgagacag caagggcagc accagtcaat aacgcaatga
     1981 ttgttctcga cgcccgtgca ccagtgacaa catcatgtaa atcgttttca ctatcaaagt
     2041 tgaataacgc ctgtatcacc gtacctggtg acacaagcgt atttccaatc attaaactta
     2101 agatagctac tattgcaaga cataaaccag caataacgat ttggtatttt ggtttaagta
     2161 gcatcgtaaa actccttaat tattttgatt gtttttcaat atttaacttt tcatataaat
     2221 cgtcaataag ttttaatgaa gatttatatc cgccagctaa gttccaagtg atttcatcta
     2281 aatcatcaga tacttggttg tttttaactg cgtctaaatt tttccactct ttacttgaag
     2341 tccattcgct ttcagtcttt ttaactaatg cagcatcttt cgcatttgga tctgatttta
     2401 ctacaaaaat atgatcagcg ttcattaatg gaatgctttc tttagatgta agttggataa
     2461 tatctttacc attatcaact tgtttttgta agtctttatt acgtttgaat cctaaatcat
     2521 ttaagatttc accagcatat ccaccagcat aaattcttgt atgatcagca cggaagttaa
     2581 caactgaagc tttcaatggc catgcatctt tatactttgc ttttgcatct ttttggaatg
     2641 cagctacttt atcatcgtac tttttaagta aatcttcagc ttctttttct ttccctaaag
     2701 ctttccccat taacttagtt gtatctttga atttgaaaac tgtatcagta gaaactgttg
     2761 gtgcgatttt agataattga tcgtaaactt tttcatttct aacttttgac gcgacaatta
     2821 agtccggttt taatttagag atttcctcta agttaggtgc aggttcttga cctacaatct
     2881 tagtatcttt taaatcattt tttatgtatt cgaatttcgg tttttgtgtc catgattcta
     2941 cagcacctac aggtttaaca cctaaagata cagcgacgtc agtggcacct tgatatagcg
     3001 taacaacacg ctttggtttc cctttaattt cagttgtacc cattgcatgt ttaattgaag
     3061 ttgtttcctt atctttgtta tcagatgatt gtttatttga attcccacta catcctgcta
     3121 aaacaagtag gaaagcaagc gtaacaacaa gcattttaat tactttattc attgactaat
     3181 tagcctcctt cgtgatgtat gacaatgaga atcattatca cgatttagta tgaattaaat
     3241 tttttcctaa gtcaataaaa tatttatgat ttacatgcaa cttataatta tttgacatat
     3301 aaatgcataa aaaatataat cctaattact tgatagtgag aatcattatc aattaggtaa
     3361 cacacaatat tatagaattt taaatttgag gaggaagcgc ttttgattga aaaaagtcaa
     3421 gcatgtcacg attcattgtt agattctgta gggcaaacac ctatggttca acttcatcaa
     3481 ctatttccga aacatgaagt gtttgcaaag ttagagtata tgaatcctgg aggcagcatg
     3541 aaagatcgac ctgccaagta catcattgaa catggtatta aacatggttt aatcactgag
     3601 aatacacatt taattgaaag tacttctggt aatttaggca ttgcgttggc aatgatagct
     3661 aaaatcaagg gattaaaact cacgtgtgtt gttgatccta aaatatcacc aacaaatttg
     3721 aaaattatta aaagttatgg tgccaatgta gaaatggttg aagaacctga tgcacatggg
     3781 ggttatttaa tgactcgtat tgcaaaggtg caagaactgt tagccactat tgacgatgca
     3841 tattggatta atcaatatgc gaatgagtta aattggcaat cccattatca tggtgcaggc
     3901 acagagattg ttgaaacaat taagcaacct atagattatt ttgtcgcgcc agtcagcacg
     3961 acaggtagca ttatgggtat gagtagaaaa ataaaagaag tgcatccaaa cgcacaaatt
     4021 gttgctgttg atgcgaaagg gtcagtcatt tttggtgaca aacctattaa tagagaatta
     4081 cctggtatcg gtgctagtcg tgtacccgaa atattgaata gatcagaaat taatcaagtg
     4141 atccatgtag atgattatca atctgctttg ggctgtcgaa aactgattga ttatgaaggc
     4201 atatttgccg gaggttcaac aggttcgatt attgcagcga ttgagcagtt gataacgtca
     4261 attgaagaag gtgcaacaat tgtcacgatt ttaccagatc gaggcgatcg ttacttagat
     4321 ttagtttatt cagatacatg gttagaaaaa atgaaatcaa gacaaggagt taaatcagaa
     4381 tgaatagaga gatgttgtat ttaaatagat cagatattga acaagcggga ggtaatcatt
     4441 cacaagttta tgtggacgca ttaacagaag cattaacagc ccatgcgcac aatgattttg
     4501 tacaaccgct taagccgtat ttaagacagg atcctgaaaa tggacacatc gcagatcgaa
     4561 ttattgcaat gccaagtcat atcggtggtg aacacgcaat ttcaggtatt aagtggatag
     4621 gtagtaagca cgacaatcca tcgaaacgta atatggagcg tgcaagtggc gtcattattt
     4681 tgaatgatcc agaaacgaat tatccaattg cagttatgga agcaagttta attagtagta
     4741 tgcgtactgc agcagtttca gtgattgcag caaagcattt ggctaaaaaa ggatttaaag
     4801 acttaacaat cattggatgc gggctaatcg gagacaagca attacaaagt atgttagagc
     4861 aattcgatca tattgaacgc gtgtttgttt acgatcaatt ctctgaagca tgtgcacgct
     4921 ttgttgatag atggcaacaa cagcgtccgg aaattaattt tattgcgaca gaaaatgcta
     4981 aagaagcagt atcaaatggt gaagtagtca ttacatgtac cgtaacggat caaccataca
     5041 ttgaatatga ttggttacaa aagggtgcat ttattagcaa catttctatc atggatgtgc
     5101 ataaagaagt ctttattaaa gctgacaaag tcgtagtaga tgactggtca caatgtaatc
     5161 gagaaaagaa aactattaac caattggtgt tagaaggtaa attcagcaaa gaagctcttc
     5221 atgctgaact aggacaactt gtgacaggtg acataccagg acgtgaagac gatgatgaga
     5281 tcatattact taatccgatg ggtatggcta tcgaagatat ttcaagtgct tattttattt
     5341 atcaacaggc acaacaacaa aatattggga caacattgaa cctatattaa gaatgcgagg
     5401 tgtctgaaca ttgcagaatc atacagcagt caatacagca caagcgataa tattaagaga
     5461 tttagttgat gcattattat ttgaagatat agccggaatt gtatcgaata gtgagattac
     5521 taaagaaaac ggacaaacgc ttttgatata cgaacgtgaa acacaacaaa taaagatacc
     5581 tgtttatttt agtgctttaa atatgtttcg ttacgaaagt tcacaaccaa ttacgataga
     5641 gggaagggtg tctaagcaac ctttaacggc agctgaattt tggcaaacaa ttgctaatat
     5701 gaattgtgat ctaagtcatg aatgggaagt ggctcgcgtt gaagaaggac tgactactgc
     5761 tgccacacag cttgctaaac aattatcaga attagattta gcgtcacatc cttttgtgat
     5821 gtcagagcag tttgcaagtt taaaagatcg tccatttcat ccattagcta aagaaaaaag
     5881 aggattaaga gaagcggatt atcaagtgta tcaagctgaa ttaaatcaat catttccttt
     5941 aatggttgca gcagttaaaa agacacatat gattcatggc gatactgcaa atatcgatga
     6001 attagaaaat ttgacagtac ctataaaaga acaagcgaca gacatgttaa atgatcaagg
     6061 gttatcaata gatgactatg tactatttcc ggtacatcct tggcaatatc agcatattct
     6121 gccgaacgtc tttgcgaaag agattagtga aaagttggtt gtactattac cgttaaaatt
     6181 tggagattat ctgtcgtctt caagtatgcg ttcattaatt gatattggcg caccgtataa
     6241 ccatgtcaaa gtaccatttg caatgcagtc attaggcgca ttaaggctaa cgcctacgcg
     6301 ttacatgaaa aacggagaac aagcagaaca attattacgt cagcttatag aaaaagatga
     6361 agcactagct aagtatgtca tggtttgtga tgaaacagct tggtggtcat atatgggtca
     6421 agataatgat attttcaaag atcaattagg tcatctaact gttcagctaa gaaagtatcc
     6481 cgaagtgcta gccaaaaatg atacgcaaca gctagtgtca atggcagcac tcgcggcaaa
     6541 tgatcgcact ttatatcaaa tgatttgtgg aaaagataat atttctaaaa atgatgtcat
     6601 gacgttattt gaagatatcg cgcaagtctt tttaaaggta acactatcat ttatgcaata
     6661 cggcgcatta ccagagttgc atggtcaaaa tatattgttg tcatttgaag atggacgtgt
     6721 acaaaaatgc gtgttacgtg atcatgatac tgtcagaatt tataaaccat ggctaacagc
     6781 acatcagctt tcattgccga agtatgtcgt cagagaagat acacctaata cgctaattaa
     6841 tgaggatttg gaaacattct ttgcttattt tcaaacatta gctgtatcgg taaatctata
     6901 tgccattatt gatgcaattc aagatttatt tggtgtaagt gagcatgaac ttatgtcgtt
     6961 gttaaaacaa attttaaaaa atgaagtggc aactatttcc tgggttacaa ctgatcagct
     7021 agctgtcaga cacattttat ttgataaaca gacgtggcca ttcaaacaaa ttttattacc
     7081 attgctatat caacgtgata gtggtggagg tagtatgcct tcaggtttaa ctaccgtacc
     7141 aaatccaatg gtgacatatg attaatcagt ctatatggcg cagtaacttt cgcattttat
     7201 ggctcagtca gtttatagcg attgctggac tgacagtact tgtgccatta ttgccaattt
     7261 atatggcatc actacaaaat ctatcagtcg tagaaataca gttgtggagt ggtatagcga
     7321 ttgctgctcc agctgtaacg acgatgatag cttcgccgat atgggggaag ctaggtgata
     7381 agatcagccg aaaatggatg gtgttaagag cgttacttgg tttggcggta tgcttatttt
     7441 taatggcatt gtgtacgaca ccattacagt ttgtacttgt gaggttattg cagggactat
     7501 ttggtggtgt tgttgatgca tcaagtgcgt ttgcgagtgc agaggcgcca gctgaagatc
     7561 gtggaaaggt attaggaaga ctgcaaagtt cagtcagcgc agggtctctt gtggggccat
     7621 taattggcgg tgttacagct tcgatattag gttttagtgc gttactgatg agtattgccg
     7681 ttattacttt tattgtctgt attttcggtg cattaaaatt gattgaaacg acacatatgc
     7741 caaaatcaca aacaccaaat attaataaag gtattcgccg ttcatttcaa tgtctattat
     7801 gcacacaaca aacatgtcga tttattatcg ttggcgtttt agcaaacttt gctatgtatg
     7861 gcatgctaac tgcattatca ccacttgctt catcagtgaa tcatacagcg atagatgacc
     7921 gtagtgtgat tggattttta cagtccgcat tttggacggc ttcgatatta agcgcgcctt
     7981 tatggggacg ctttaatgat aaatcatatg ttaaatcagt atatatattt gccacgattg
     8041 catgtggttg tagtgcgata ctgcaaggtt tagcgacgaa tatagagttt ttaatggctg
     8101 caagaatact tcaaggatta acatatagtg cattgattca aagtgtcatg tttgttgtcg
     8161 tgaatgcgtg tcatcaacaa cttaaaggca catttgttgg aacgacgaac agtatgttag
     8221 ttgttggtca aattattggc agtcttagtg gcgctgccat tacaagttat actacaccag
     8281 ctactacgtt tatcgttatg ggcgtagtat ttgcagtaag tagtttattt ttaatttgtt
     8341 caaccatcac taatcaaatc aacgatcaca cattaatgaa attatgggag ttgaaacaaa
     8401 aaagtgcaaa ataaagaatt aatacaacat gcagcgtatg cggctatcga acgcatttta
     8461 aatgaatatt ttagagaaga aaatttatat caagtaccac ctcaaaatca tcaatggtct
     8521 atacaattat cagagctcga aactttaacg ggtgaatttc gctattggtc tgcgatgggg
     8581 catcatatgt atcatccaga ggtatggctt atcgatggaa aaagtaaaaa aataacaact
     8641 tataaagaag caattgcgcg tattttgcaa catatggctc aaagtgcaga taatcaaacg
     8701 gcagtgcaac aacatatggc gcaaattatg tctgacatcg ataatagcat tcatcgcacg
     8761 gcacgttatt tgcaaagtaa cacaatagac tacgtagagg atcgttatat cgtttcagaa
     8821 caatctttat acttaggtca tccatttcat ccgactccta agagtgcaag tgggttttca
     8881 gaagcagatt tagagaaata tgcacccgaa tgtcatacat cattccaatt gcattattta
     8941 gctgtgcatc aagatgttct gctcacgcgc tatgtagaag gtaaagaaga tcaggttgag
     9001 aaagtgttgt atcaattagc agacatagat atatcagaga tacccaaaga ttttatttta
     9061 ttaccaacac atccttatca aatcaatgtg ttgcgacagc atccacagta tatgcaatat
     9121 agtgaacaag gtttaataaa agaccttggc gtttccggtg attcagtgta cccgacgtct
     9181 tcggttagaa ctgtattttc aaaagcatta aacatttatt taaaattacc gatacacgtt
     9241 aaaatcacta attttatacg tacgaatgac cttgaacaga ttgaacggac aattgatgcc
     9301 gcgcaagtta tcgcatcagt caaagatgag gttgaaacac cccattttaa attgatgttt
     9361 gaagaaggat atcgtgcatt gttaccgaat ccattagggc aaacagttga acctgaaatg
     9421 gatttattaa caaatagtgc catgattgtt cgtgaaggga taccgaatta ccatgctgat
     9481 aaagatattc atgtattggc gtcattattt gaaacgatgc ctgattcacc gatgtctaag
     9541 ttatcacaag tgattgagca aagtggttta gcaccagaag catggcttga atgttatttg
     9601 aatcgtacat tattgccgat attaaagctg tttagtaaca caggcattag tctagaagca
     9661 catgtacaaa atacattaat tgaattaaaa gatggcatac ccgacgtatg ctttgtcaga
     9721 gatcttgaag gcatttgtct atctagaacg attgctactg aaaaacagct tgtgccaaat
     9781 gttgtggcag catcaagccc tgttgtatat gcacatgatg aagcatggca tcgtcttaaa
     9841 tattacgttg tagtaaatca cttaggacat ttagtatcaa ctattggtaa agcgactaga
     9901 aatgaagttg tgttatggca acttgtagcg catcgtctta tgacttggaa aaaagaatac
     9961 gcgaataacg cagtatttgt tgactgtgta gaagatttat atcaaacgcc gaccattgcg
    10021 gctaaagcga atttgatgag taaattgaat gattgtggtg caaaccctat ttatacacat
    10081 ataccaaatc caatttgtca taacaaggag gtatcgtatt gtgaatcaaa caattcttaa
    10141 tcgtgtaaag actagagtga tgcaccaact ggtatcatca cttatttatg agaatattgt
    10201 tgtgtataaa gcgtcatatc aagacggtgt cggtcatttt acaatagaag gacatgattc
    10261 agagtatcgt tttactgctg aaaagacaca tagctttgat cgtatacgta tcacatcacc
    10321 aattgagcgt gtcgtaggag atgaggcaga tacaacaaca gactatacac aattattgag
    10381 agaggttgta tttacatttc ctaaaaatga tgaaaagcta gaacaattta ttgtcgagtt
    10441 attacagaca gaattaaaag atacgcaaag tatgcagtat cgagaatcaa acccaccagc
    10501 aacacctgag acatttaacg actatgaatt ttatgcgatg gaagggcatc agtatcatcc
    10561 aagttacaaa tcacgtttag gatttacgtt gagtgataat ttgaaatttg gtcctgattt
    10621 tgtaccaaac gttaaactgc agtggttagc tatcgacaaa gataaagtag aaacgacggt
    10681 atcaagaaat gttgtagtta acgaaatgtt acgtcaacaa gttggcgata agacttatga
    10741 acattttgta cagcagattg aagcatctgg caaacatgta aatgatgttg agatgatacc
    10801 tgtacaccca tggcagtttg aacatgtcat ccaagttgat ttggctgaag aaaggcttaa
    10861 tggcacagta ctatggttag gggaaagtga tgagctatat caccctcaac aatcgattcg
    10921 tacgatgtcg ccaatagaca cgacaaaata ttatttaaag gtaccaataa gtataacgaa
    10981 cacttcaacg aaacgagtgt tggcgcctca tacaattgaa aatgcagcgc aaattacgga
    11041 ttggttaaag cagatacagc aacaagatat gtatttaaaa gatgaattaa agacagtttt
    11101 tctaggggaa gtcttaggac agtcttattt aaatacacaa ctttcgcctt ataaacaaac
    11161 tcaagtttat ggtgcgttag gtgttatatg gcgtgaaaat atatatcata tgttaatcga
    11221 tgaagaggat gcgataccat ttaatgcact ttatgcaagt gataaggatg gtgtaccatt
    11281 cattgaaaat tggattaaac aatatggttc tgaagcttgg acaaagcaat ttttagctgt
    11341 agcgattcgt ccaatgattc atatgcttta ttatcacggt attgcctttg aatcgcatgc
    11401 acaaaatatg atgctcattc atgaaaatgg ttggcctaca cgtattgcct taaaagattt
    11461 ccatgatggt gttcgtttta agcgtgagca tttaagtgaa gctgcttcac acctgacatt
    11521 aaagccaatg ccagaagcac ataaaaaagt gaatagtaat tcatttattg aaacagatga
    11581 cgaacgttta gtacgcgact ttttacatga tgcatttttc tttattaata tcgccgaaat
    11641 catcttattt attgaaaagc aatatggtat cgatgaggag ctgcaatggc aatgggttaa
    11701 aggcatcatc gaggcgtatc aagaagcatt tccagagttg aataactatc aacatttcga
    11761 tttgtttgaa cctacgattc aagttgaaaa gttaacgaca cgtcgattat taagtgactc
    11821 cgagttaaga attcatcatg ttacaaatcc attaggtgta ggaggtatca atgatgcaac
    11881 aactatctct gaaacataga ttaaacaatg gtgattcagt ttatggcatt tttaattcta
    11941 taccggaccc attgatgatc gaggttatcg cagcaagcgg gtatgacttt gttgtgattg
    12001 atacagaaca cgtggcgatt aatgatgaga cactagcgca tttaattcgt gcagctgaag
    12061 cagcgcatat tataccaatt gtacgtgtca ctgcagtgat agatagagat atcattaaag
    12121 tgttggatat gggtgcgaga ggtattattg tgccacacgt taaagatcgt gagacagttg
    12181 agcatattgt gaaattaagt cgttattacc cgcaaggatt aagaagtttg aatggtggtc
    12241 gcatggcaag atttggacgt acaccattac ttgatgcaat ggagatggct aatgagcata
    12301 ttatggtgat tgccatgata gaagatgttg aaggggttat ggccattgac gatatagcac
    12361 aagtcgaagg tttagacatg atagtcgaag gtgccgcaga tttatcgcag tcacttggca
    12421 taccatggca aacgcgtgat gatcaagtaa catcacatgt tcaacatatt tttgaagttg
    12481 tgaatgcaca tggtaaacat ttttgtgcat taccacgtga agatgaagat attgcaaaat
    12541 ggcaggcaca aggtgtacaa acatttattt taggtgatga tcgcggaaaa atatatcgcc
    12601 atttaagtgc atctctagcg acgtctaaac agaaagggga tgaaggctaa tgcgtatagt
    12661 tcaacctgtt attgaacaat taaaagcaca atctcatcca gtttgtcatt atatctatga
    12721 tttagtcgga ctggaacatc atttgcaaca tattacatcg tcattgccga gtaattgtca
    12781 aatgtactat gcaatgaaag caaatagtga acgaaaaatc ctagatacaa ttagtcagta
    12841 tgttgaagga ttcgaagttg catctcaagg tgaaatagca aaaggtcttg cttttaaacc
    12901 agcaaatcat attatttttg gtggccctgg taagacagac gaggaactaa gatatgcagt
    12961 aagtgaaggt gttcagcgta ttcatgttga aagtatgcat gaattacaac ggctaaatgc
    13021 catcttagaa gatgaagata agacacaaca cattttattg cgtgttaatt tagcaggacc
    13081 atttcccaat gcaacgttgc atatggcagg acgcccaaca caatttggta tttctgaaga
    13141 cgaagttgat gatgtcattg aagctgcgct cgcaatgcca aagattcatc tagatggatt
    13201 tcattttcat tctatttcta acaatttaga ctcgaattta catgtcgatg tagtgaaact
    13261 ttattttaaa aaagcaaagg catggtctga aaaacatcga tttccactca aacatatcaa
    13321 tcttggtggt ggcataggcg ttaactatgc agatttaact aaccaatttg aatgggataa
    13381 ttttgtagaa cgttttaaaa cacttatcgt tgagcaagaa atggaagatg tgacattgaa
    13441 ctttgaatgt gggcgcttta ttgtggcaca tattggttac tatgtgacag aagtgctaga
    13501 cattaagaaa gtacatggtg cttggtatgc catattaaga ggaggtacgc aacaatttag
    13561 actgccggta tcttggcaac ataaccatcc ttttgacatt tatcgctata aggacaatcc
    13621 atattcattt gaaaaagttt caatttcgag acaggacaca acgttagtcg gtcaattatg
    13681 tacaccgaaa gatgtctttg ctagagaagt acagatagac gcaatcagta caggcgacgt
    13741 tattgttttc aaatatgcag gtgcatacgg atggtctatt tcacatcacg atttcttaag
    13801 ccatccacat cctgaattta tttatttaac gcaaacaaag gaggatgaat aactattgaa
    13861 tcatattcat gaacatttaa aattggtacc agtagataag attgatcttc acgaaacatt
    13921 cgaaccttta agattggaaa aaacgaaaag tagtattgaa gcagatgatt ttatacgtca
    13981 tcctatttta gtgacagcga tgcaacatgg tagatatatg gttatagatg gtgtgcatcg
    14041 gtatacaagt ttgaaagcgt taggatgtaa gaaagttcca gtgcaagaaa tccatgaaac
    14101 acaatattca attagtacat ggcaacataa agttccattt ggtgtgtggt gggaaacgtt
    14161 acaacaagaa catcgcttgc catggactac tgagacaaga caagaagcgc catttattac
    14221 aatgtgtcat ggtgatacag aacaatattt gtatacaaaa gatttaggcg aagcacattt
    14281 tcaagtatgg gaaaaggttg tcgcaagtta tagtggttgt tgttctgtag agagaattgc
    14341 acaaggtaca tatccttgtc tttctcaaca agatgtactc atgaagtatc agccattgag
    14401 ttataaggaa attgaagcgg ttgttcataa aggggaaact gtgccagcag gtgtgacacg
    14461 ctttaatatt tcaggacgat gtcttaatct tcaagtacca ctggcattac ttaaacaaga
    14521 tgatgatgtt gaacaactgc gcaattggaa gcagttttta gcagataagt ttgccaatat
    14581 gagatgctat actgaaaaag tatacttggt ggagcaatag ttttactgtg atgttgaggg
    14641 aaatatgatg atttagcgta ttgatagcga aaatataata aaacaatata gtgtggagaa
    14701 cttttgatat tttataaata ttgaagttct ccatttttgt attttgcata t
//
LOCUS       DAMQYT010000050        14538 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMQYT010000050 DAMQYT010000000
VERSION     DAMQYT010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14538
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.050"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            278..3232
                     /gene="fdhF"
                     /locus_tag="PGA33_001160"
     CDS             278..3232
                     /gene="fdhF"
                     /locus_tag="PGA33_001160"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDG6472985.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTAIKEKHGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPESELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRQPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            3232..3705
                     /locus_tag="PGA33_001161"
     CDS             3232..3705
                     /locus_tag="PGA33_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HDG6472986.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDDDMNRSLTYMLNMLKGMSREE"
     gene            3933..4166
                     /locus_tag="PGA33_001162"
     CDS             3933..4166
                     /locus_tag="PGA33_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472987.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            4188..4964
                     /locus_tag="PGA33_001163"
     CDS             4188..4964
                     /locus_tag="PGA33_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDG6472988.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            5275..6399
                     /locus_tag="PGA33_001164"
     CDS             5275..6399
                     /locus_tag="PGA33_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDG6472989.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLFVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(6491..7444)
                     /locus_tag="PGA33_001165"
     CDS             complement(6491..7444)
                     /locus_tag="PGA33_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HDG6472990.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(7806..8306)
                     /locus_tag="PGA33_001166"
     CDS             complement(7806..8306)
                     /locus_tag="PGA33_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDG6472991.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
     gene            complement(8543..8965)
                     /locus_tag="PGA33_001167"
     CDS             complement(8543..8965)
                     /locus_tag="PGA33_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HDG6472992.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDIDFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYYNGQEYIIPLTIRFI"
     gene            9213..10295
                     /locus_tag="PGA33_001168"
     CDS             9213..10295
                     /locus_tag="PGA33_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HDG6472993.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
     gene            10390..11790
                     /gene="nhaC"
                     /locus_tag="PGA33_001169"
     CDS             10390..11790
                     /gene="nhaC"
                     /locus_tag="PGA33_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDG6472994.1"
                     /translation="MMKRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNESMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVEEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            11964..12206
                     /locus_tag="PGA33_001170"
     CDS             11964..12206
                     /locus_tag="PGA33_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6472995.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKALETLLA"
     gene            complement(12223..13026)
                     /locus_tag="PGA33_001171"
     CDS             complement(12223..13026)
                     /locus_tag="PGA33_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDG6472996.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNNYNNGYSYNNYSRYN
                     NYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSGYT
                     SGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQTTQ
                     GAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            13636..>14538
                     /locus_tag="PGA33_001172"
     CDS             13636..>14538
                     /locus_tag="PGA33_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDG6472997.1"
                     /translation="MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDL
                     KRNYVTIIMLNDLYQIDGNDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIY"
BASE COUNT         5051 a         2018 c         2726 g         4743 t
ORIGIN      
        1 aagatatggc aaaaagcgtg aaacagataa ttggaaaact aataatgata attattttca
       61 attaaacaaa aagatgattt aatttcgcgc aaaatatttc aaataaaaat gattgtaaaa
      121 aggcaaaata caaaatttta cttaacaact agtacataaa gtaatacaat taaattaatt
      181 ctatctgaaa gatgtgtggg gcatcgttat tttaggtgga tatgagcaat ttattaaaag
      241 tcatttacgg aaaatatata tagacggggt gagtaatatg caagaacatt tggtggttac
      301 acttgatgga aaagattatc ttgtagaacc aggtacgaat ttacttgaat ttattaaatc
      361 acaagatact tttgtacctt caatttgtta taacgagtcg atgggaccaa ttcaaacatg
      421 tgatacatgt actgttgaga ttgacggtaa aattgaacgt tcatgtagta cggtgattga
      481 tcgtccaatg actgtaaata ctgtgaacaa tgatgtgaaa gatgctcaaa aagaagcgct
      541 tgatcgaatt ttagaaaagc atatgctgta ttgtacagta tgtgattata ataatggtga
      601 ttgtgaaatt cataatacga tggatgcatg ggggcttcag catcaaacgt atgaatataa
      661 agagaagcca tacgaaaaag attacggccc attttatcgt tatgatccaa atcaatgtat
      721 cttatgtggg cgttgtgtag aagcatgtca agatatcgaa gtgaatgaaa ctattagaat
      781 tgattgggat cgtgaacatc cacgtgttat ttgggataat gatgtaccga ttaatgagtc
      841 ttcatgtgta tcttgtggtc aatgtgcgac ggtatgtcca tgtaatgcca tgatggaagt
      901 gaatatggaa ggtaatgcgg gttatatgac tgatacggaa cctggttcat tagcagcaat
      961 gattgattta actaaaaaag cagaacctgg ttatggtcca ctatttgcaa tctcagattc
     1021 agaagcagaa atgcgtaaag agcgtattaa gaaaactaaa acagtgtgta cttattgtgg
     1081 tgtgggttgt tcatttgaag tttggactaa agatagagaa attttaaaag tacaaccatc
     1141 acatgattct ccagcaaata aaattgcgac ttgtgttaaa ggtaagtttt catggggaca
     1201 tattaattca gatcaacgat taactaaacc actagtaagg aaaaatggtg agttccatga
     1261 agtagaatgg gatgaagctt taaacgtcat tgcagataat tttacagcta ttaaagaaaa
     1321 gcatggccca gatgcactat cattcatttc ttcttctaaa gcgacgaatg aagaatcgta
     1381 tttaatgcaa aaattagcaa gacaagttat tggcacaaat aacgttgata actgttcaag
     1441 atattgccaa gcacctgcaa caaaaggctt atttagaacg gttggacacg gcggtgactc
     1501 aggtagtatt gaagatttag aaaaagcggc aatgtctgta ttgataggta ctaatacagc
     1561 cgaagctcat ccagttatcg catcacgcat gaaacgtgca caaaaattat ttggtcaaaa
     1621 aatacatgta tttgatatta gaaaacatga aatggcagaa cgtgcggatc gtttttatca
     1681 acctaaacca ggtacggatt tagcgtggtt aagtgcagtg actaagtata ttattgatca
     1741 tgatttacac gataaagcat ttattgatga gtgggtagat gattttgatg aatattacaa
     1801 atcattagaa acatttacaa tggcttttgc tgaagaagca acaggtattc ctgaatcaga
     1861 attgattaaa tttgctgaag aatgtgctaa agctgaatct gttgtaattt gttgggcaat
     1921 gggtattaca caacaagaca ttggtagtga ctcaagtaca gcgatttcaa acttattatt
     1981 agtaacaggt aattatcgtc gtcctggtac tggtgcgtat ccattacgtg gacataataa
     2041 tgttcaagga tgtagtgata tgggaagtat gcctgataag attactggtt atcaaagtat
     2101 tgaagcggat gatattcgcg ctaaatttga aaaagaatat ggcgttaaat tgaatccaaa
     2161 agctggtaaa gataatcatg aaatggtaga aggtatacat gacggagaag tacactcatt
     2221 gtacttatat ggtgaagata cgggtattgt ggattcaaat attaattttg tacaagctgc
     2281 gtttgaaaaa ttagatttca tggtagtcca agatgaattt ttaacattca cagcaacata
     2341 cgcagatgtt gtattgccag caagtccttc acttgaaaaa gacggtactt ttacaaatac
     2401 cgaacgtcgt attcaacgtt tataccaagc attagaacct cttggtgatt caaaacctga
     2461 ctggaaaatt ttccaagcaa ttgctaatag attaggattt gattggaatt acaagcatcc
     2521 tagtgaaatt atggatgaag tagcacgctt aacacctcta tatgctgggg taagttatga
     2581 tcgtttagaa ggattcaata gtttacaatg gccagtacaa cctgatggta ctgatgagcc
     2641 tattctatac ttagaaggat tcaattttga taatggtaaa gcaaaattat tcccattatc
     2701 atttgataat tactttaagc aagatgaaat ttatgatatt catgtaaaca atggtcgatt
     2761 gttagagcac ttccatgaag gtaatatgac ttatcaaaca ccaatgatta aatacaaagt
     2821 gccacgtgca tttgttgaaa tttctccaga gcttgctgaa gatagaggca ttcatgaagg
     2881 tgcagaagtt aagctgattt ctgaaacagg agaagcggta ttacaagttc acgtgacaga
     2941 tcgtgttaaa ggtaaagaaa tctatattcc attaaataat gatgcgatgg aaaatggaga
     3001 tctaggtgca attaacttat taactaatag tgatgttgat caatacacgg atacaccatc
     3061 ttataaacgt actagctgtc gtttggaggt aattacgaaa cgtggtaagt caccgttgaa
     3121 ccctaataat ttccgtgtca ataaaaaacg tcaaccgcag tacagtgttc aagtacagaa
     3181 aaaatgggaa cgttcagatt atgttttccc gggaaatcag gtggataaat aatggctgag
     3241 agaatatctt ctaaaattag acgcttagaa aaatcagaag aacaaattaa gttagaaagt
     3301 ttaaatgaag ttacagaggc aattgcagcg aataaagata gtatattaaa agcaatcaag
     3361 cttattaaaa cattagatga tgcaaaacta ttagatgctt taaatggtgc aattcgagga
     3421 cgtcaagtca tcataaataa atttgcagtt gaacttaata aagatattta tacagggcta
     3481 ttatctaata tggcttcaat ggtattttta ttaggtgaac tcaatgtatc agatttaagc
     3541 gacttcctaa ataaagttaa taaaggatta cacgttgcca atcaagcgag tccaaatgct
     3601 aagacaacta taagaagtct attgggcgta ttgaaagatg acgacatgaa ccgtagttta
     3661 acatacatgc ttaatatgct aaaaggtatg tctcgagaag aataacatta caatgaaaaa
     3721 gtgtttctac atgcaagcag tgtaggagca ctttttttaa tttttgtaaa aattaaaaag
     3781 ggtgtttttg atggaataaa tgaataatat tgaaaaacaa aaaaatcacg tgctattgtg
     3841 aatcaatcat cgacaggcct atgaaacatc atgtattatt atgattatgt aagttattta
     3901 aataaaaagg gtgagtgcat attaattcga gaatgttaaa aacgtcagct gtaatttcta
     3961 atacattatt agtcataggt ttagtctgtc tatttatgat gaaattagtg ttagctatta
     4021 ctttttttgc tgtttcatta tcaattagtc tagttgtctt taatatgatg ttcagagata
     4081 gaacaacgat gaaagtaatc gttaacgctt cttttttaat cgtaatatta gcgattgtag
     4141 tggcatattt tttattgtct aagtaagaaa aatgaggttt tgtctatatg aagaagaatt
     4201 tcaagttacg catttcaacg ctactattga tagttatttt agttgttttt gctgtattac
     4261 tcatcgtgaa tgaaactaaa ttgtttaaaa atgatgtgaa ttactctttt gatgaggctg
     4321 tttcaatgca acaagggaaa ggtattgtac agacaaaaga agaggatggt aaatttgttg
     4381 aagcaaataa taatgaaatt gctaaagcaa tgactatttc acataaagac aatgatatga
     4441 agtatatgga tatcacagaa aaagtgccaa tgtcggaatc tgaagttaac caattgctaa
     4501 aaggtaaggg gattttagaa aatcgaggga aagtttttct agaagctcaa gaaaaatatg
     4561 aggttaatgt catttatctt gttagccatg cattagtaga aacaggtaac ggcaaatcag
     4621 aattagcaaa aggcattaaa gatgggaaaa aacgctatta caactttttt ggtataggag
     4681 cattcgatag tagtgctgtt cgtagtggga aaagttatgc tgaaaaggaa caatggacat
     4741 caccagataa ggcgattatt ggtggtgcaa agttcattcg taatgaatat tttgaaaaca
     4801 atcaactgaa tttatatcaa atgcgatgga atccagaaaa tcctgcgcaa catcaatatg
     4861 cgagtgacat tcgctgggca gataaaattg ccaaattaat ggataaatcc tataagcagt
     4921 ttggtataaa gaaagatgat attagacaaa catattataa ataagacatc ggtgcttaaa
     4981 ggagctggaa caatttattg tttcgagctc ctttagcgca ttctgagtgt gttagttaaa
     5041 tggattttaa cctaacaaaa aacgctatat agcatcaaat atgctatatc ccacatcatt
     5101 gttacaaatg tacatgatgt aaatgaatat tgctgtctaa atgtgcatgt aatatacaat
     5161 ggtgcagata atacacttaa gtccttaaaa atgaaacgtt agttccaaga gtcattttta
     5221 aacaatagtg catgtgataa aatagaaaag aatgaaaaat atagaggtga caatatgaag
     5281 atagcaatta taggtgcagg catcggtgga ttaacagctg ctgcattatt acaagaacaa
     5341 ggtcatacta ttaaagtctt tgaaaaaaat gagtcagtta aagaaattgg cgctgggatt
     5401 ggtatcggag ataatgtgct taaaaaacta ggtaatcatg acttagctaa aggtattaaa
     5461 aatgctgggc aaatcttatc tacaatgaca gtgttagatg acaaagatcg cctgttaact
     5521 actgttaaat taaaaagtaa tacattgaat gtgacgttac cacgccaaac attaattgac
     5581 attattaaat cttatgtaaa agatgacgca atatttacaa atcatgaagt cacgcatata
     5641 gataatgaga cagataaagt taccatacat ttcgcggaac aagaaagtga agcatttgat
     5701 ttatgtattg gtgctgatgg aattcattct aaagtgagac aatctgtaaa tgctgacagt
     5761 aaagtattat atcaagggta tacatgcttt agaggtttaa ttgatgatat tgatttaaag
     5821 catccagatt gtgcaaaaga atactgggga agaaaaggaa gagtaggtat tgttccgtta
     5881 ttaaataatc aagcatattg gttcattaca attaactcga aggaaaacaa tcataaatat
     5941 agttcgtttg gtaaacctca tttgcaagca tactttaatc actatccaaa tgaagttaga
     6001 gagatcttag acaaacaaag tgaaacaggt atcttattgc ataatattta tgatttgaaa
     6061 ccactcaaat cttttgttta tggtcgtact attttactag gagatgcagc acatgcgaca
     6121 acgcctaata tggggcaagg tgctggacaa gcaatggaag atgctatcgt attagtaaat
     6181 tgttttaacg catacgactt tgaaaaagca ttacagcgtt atgataaaat acgtgtcaaa
     6241 catactgcaa aagtaattaa gcgttctaga aaaatcggta aaattgccca atatcgtagt
     6301 cgtttatttg ttgcagttag aaatcgtatt atgaaaatga tgccaaatgc attagcagct
     6361 ggacaaacta aattcttata taaatcgaaa gaaaaataat acaacaatat gaaaaccccc
     6421 gtatgttgaa acgagagctc aacatatggg ggttcttgtt tttataatgt tattataata
     6481 aattcaatta ttagttaacg acaaattgtg gtttctcacc ttgaacggca ctaattgcag
     6541 cattagcaac aattttagac atcatgtcac gtgcttcaaa tgtagcatta ccaatatgcg
     6601 gtgttaatac tacattatta agtgatttta agtcatcggt aatatctggt tcaaattcat
     6661 atacatcaag tgcagcacct tcaatttcat tatctttcaa tgcttgcact agtgcttgtt
     6721 cgtgcacgat tggaccacga gaggcattga ttaaatacgc cgtagatttc atcattttaa
     6781 attgttctgt atcaattaaa tgatgcattt taggattata agcagcgttg atagtgataa
     6841 aatctgcatt ctttaatagt gtatctaaat ctacatattt tgcaccgatt tctcgttctt
     6901 tttcttcttt gcgattaggt ccagtgtata gcacatccat gtcaaatgct cttgcacgac
     6961 gagctactgc actaccaatt tcacctaaac cgataatgcc gattgttttc ccagatactt
     7021 ctctacctct gaaaaataaa ggtgcccatc catcaaatcc agttgtacgt gataattggt
     7081 ccccttcaac aatacgacgc gctactgcaa gtactaatcc aattgttaaa tcagcagtcg
     7141 cgtttgttga tgctttaggt gtgtttgtaa catctatact tttttctcgg gcatactcga
     7201 tatcaatatt attaaaacca gcgccatagt tggcaatgat ttttaagtct ttaccagcat
     7261 cgataacatc tttatcaacg tttgtagata ataaactaat taaggcagtc gcgtttttaa
     7321 cacctttaat taaagtgtct ttatcgacta atcctttacc ttcatacatt tcaacttcaa
     7381 aatgttcttg taaaagtttt aaacctactt ctggtattgc accagcaaca taaacttttt
     7441 ccataaaaga tcactccttt tatcttagta tagtagaaga ttagacagta tacaactatg
     7501 tcatgatgtc ttgtgtatca atgatgtaag cgcgtacttt tgatggaggt gatataactt
     7561 aggcactgta gaactatgaa tattgtaatg tggaaaaact ggatcaatta aattagataa
     7621 cgtagtttta aagttaatag tattagaaaa aattaatatt ttgaatatgg gaggaaatat
     7681 aaataagtag gtggcaacga aaaatagcaa aaaaagagct tctcctataa aggaaagctc
     7741 aaagtttttt gatgacatat gtactagaat taagtttcaa gacaatatgt atcatcgtgt
     7801 ttatattaaa tatggatgta gttgtagtta cctgcttcac ttgcagaaat agttctagaa
     7861 cttactgaga aaggtccgcc actataattc atttctgaaa ttgtaactga accatcactg
     7921 tttacacttt ctacatatgc aacgtgacca aatggtcctt cagaagattg taggatagcg
     7981 cctttagaag gtgtatgatt tactgtgaat ccagcacctt gtgcagcagc agcccaatta
     8041 ttagcatttc cccaagtaga accgatttct ccgccaactt tatcatatac ataccaagta
     8101 cattgtcctg cagtgtataa gttaccagaa tgtgaaattg atgatgtagt tgtcgtagtt
     8161 gtcgtagtcg ttgtagtttg agtcgtgttg tagttatagt tgttgtaatt tgtataattt
     8221 tcagcagcat ctgcatgatg tgcttgacct actaatgctg tgccgattcc tgctgttaac
     8281 gtagttgctg ttactaattt tttcatgaat aaagtcctcc aaagttctat atcttttttt
     8341 ataaataaaa cgtagcgact gttttattct cacatctcga attgatgaca atagttactt
     8401 taacaaaatt aatgcttctt gtggggaatg ttattgattt gtaaaagaat aaaaaaactt
     8461 tgactaattt tgtaataaaa attagtcaaa gttacaatga gattaacaga taattaatag
     8521 gaaatattta tttgtaatat gtttaaataa atcgaattgt taaaggtatt atatattctt
     8581 ggccattata atatttgaca cacgcaataa ttgtgaatac aaaagataat attgagaaag
     8641 cgaatatgga taaaataccg ataaacgtaa tgatgaaacc tataataata atgaaatcaa
     8701 tatctgtagc aattaggaaa acgcctatta aagtgataac gactaaaacg atagaccaaa
     8761 taatataaga aatcgtatag ttaagataat tttttccagc acgatcaact agtttcgatt
     8821 catctttttt caataaccat attatcagtg gaccaataat agatgtgaat aaacttaata
     8881 aatagataag catcgccata atgttctcat cattggattt gcgattcggt tgatgatttg
     8941 ttacgtcgtt catttcagtt gtcatattag acactccttt gaaaattgta atattatctt
     9001 taactataac aaaatataat caaaaataaa catgtttatt aaacaattat taaaaataaa
     9061 aataattggt ggacgtcggc gtttaaatag gttaatttaa ggttatatat acttaacatt
     9121 tataatgatg cgtaatgaat tcgcatcatt tttatattgt cttacgtata atttgttttt
     9181 aattttaacc aaagatagaa agagggttgt ttatgaaaat agcaattgta ggatcaggaa
     9241 atggcgcagt tacggcagca gtagatatgg tgagcaaagg ccacgatgtt aaattatatt
     9301 gtcgtaatca atctataagt aagtttcaaa acgcaatcga aaagggcgga tttgatttta
     9361 ataatgaagg tgatgaacgt ttcgtaaaat tcactgatat tagtgatgat atggaatatg
     9421 ttttaaaaga tgctgaaatt gttcaagtga ttattccatc ttcatacata gagtattatg
     9481 ctgatgtaat ggcagagcat gtaactgata atcagttgat attcttcaac atggctgcag
     9541 caatggggtc aattcgtttt atgaatgttt tagaagatag acatattgaa acaaaaccac
     9601 aactagcgga agctaatacg ttgacgtatg gtacgcgtgt cgattttgaa aatgcagcag
     9661 ttgatttatc tctaaatgta cgtcgtatct tcttttcaac atatgataga agctgtctaa
     9721 atgattgtta tgacaaagtt tcaagtattt atgatcattt agtaaaagag gaaagcttaa
     9781 ttaaaacaaa tcttgaaaat ggtaatcctg aagtgcatcc aggaccaaca ttattgaatg
     9841 tcggtcgcat tgattatgct ggcgagttcg ctttatataa agaaggaatt actaaacata
     9901 cagttagatt acttcatgca atcgaattag aacgtttgaa tttaggccgt agattaggtt
     9961 ttgaattatc aacagctaaa gaatcacgta ttgaacgtgg ttatttagaa cgtgataaag
    10021 aagatgaacc attaaatcgt ttgtttaata caagcccagt attttcacaa attccaggac
    10081 caaatcatgt agaaagcaga tatttaactg aagatattgc atatggttta gtactatggt
    10141 caagcttagg tcgtgttatt gatgtaccga caccaaatat agatgcagta attgtaattg
    10201 catcaaccat tttagagaga gacttctttg aggaaggctt aacagttgaa gaaattggtt
    10261 tagataagct tgatttagaa aaatatttaa aataaatgat ggcttgaaga tagaaaagga
    10321 tatagcatta tgcaaaagca ataaattgaa gaaaagaggt ttctcatcaa taagcgaagg
    10381 ggacgataga tgatgaaaag aaaacccacc tttttagaat caatttcgac aatgattgta
    10441 atggttattg ttgttgtaac aggctttgtg ttttttgata ttccaattca agtattatta
    10501 attattgcct cagcatatgc cacatggatt gcaaaacgtg taggcttaac atggcaagat
    10561 ttagaaaaag gcattgcaga acgtttaaat actgcaatgc ctgcaatttt aattatacta
    10621 gcggtaggaa ttatagtagg cagttggatg ttttctggca cagtgccagc cttgatttat
    10681 tatggcttag atttattgaa tccaagctat tttttaatat cagccttttt tataagtgct
    10741 gttacatctg tagcaactgg tacagcatgg ggctctgcat caactgcagg gattgcactt
    10801 atttctattg gtaatcaatt ggggattcct ccagggatgg cagcgggtgc tattatagca
    10861 ggggctgtgt ttggcgataa aatgtcacca ttatcagata caactaattt agcggcgctt
    10921 gttactaaag ttaatatatt taaacatata cattcgatga tgtggacgac gatacctgca
    10981 tcaatcatag gtttattagt atggtttatt gctggatttc aatttaaagg gcattcaaat
    11041 gataaacaga ttcaaacttt gttatcagag cttgcacaga tttatcaaat taacatatgg
    11101 gtctgggttc ccttaattgt gatcattgtt tgtttgctat ttaaaatggc tacagtgcca
    11161 gctatgctaa tatcaagctt ttctgccatt atagtgggga cttttaatca tcatttcaaa
    11221 atgacagatg gtttcaaagc aacatttagt ggttttaacg aatcaatgat acatcagtct
    11281 catatttcat ccagtgtgaa aagcttgtta gaacagggtg gtatgatgag tatgacccaa
    11341 atattagtaa cgatattttg cggatatgca tttgcaggta ttgtagaaaa agcaggatgt
    11401 ttagaagtct tattaactac tatttctaaa ggcatccatt ctgtaggaag tttaatatgt
    11461 attactgtta tttgttgtat tgcgcttgta ttcgctgcag gtgttgcttc gattgtaatt
    11521 attatggtcg gtgtgttaat gaaagatttg ttcgaaaaat accaagtctc tcgttccgtt
    11581 ttatcaagaa cacttgaaga ctcaagtacg atggtcttac ctttaatacc atggggaaca
    11641 tcaggtattt actatacgaa tcaacttcat gtctctgttg aagaattttt catatggaca
    11701 gtaccatgtt atttatgcgc aattatagca attatctatg gttttacagg gataggtatt
    11761 aaaaagtcat cgaattcacg tttaacttaa tgtgagcgtg gaatatatat aatatgttga
    11821 aacactttaa tcatttataa ttgtagcggt tataatttga aaaggtttta acttagaata
    11881 aatatcctct atacatatac tgaatatgtt ttgtagcgga acatgttgat atatgtaatg
    11941 taagttttat gtcatgattt gtaatgacta aattaattga gaatttgaag gcaagtatat
    12001 ttgtaagtac tttaactaaa aatttatcaa tgtatagccg atttgacatg cctaaatttg
    12061 ggtgtgtcaa tggctgtatg ttgtttattc tttattacag agtgaatcgg attggtgaaa
    12121 atcgaaattt tgagattttt accaattcga tttttttcat agaaattaaa aaagccaaca
    12181 aggctcttga aaccttgttg gcgtaaacat agccatcact aattagtgaa tgaagttata
    12241 accagcagct tggctagctg agattgtacg tgaagttaca acacctgggc cataaccata
    12301 gttcatttct gaaactctta ctgaaccatt gctgttaaca ctttcaacgt atgcaacgtg
    12361 accgtatgca ccttgagttg tttgcataat tgcaccagct tttggtgtat tgttcactgt
    12421 gtaaccagct cttgcagctg cgttagccca gttacttgca ttgccccaag ttgaaccgat
    12481 tttaccacct acacgatcaa atacgtagta tgtacattga ccagaagtgt ataagttacg
    12541 tcctgaagta taaccacttg agattgaacg gccatttgat gatggagcca tagttgtagt
    12601 tacttgaaca ttgttgcttg aagtgctgta gcttgcacct aaaccaccag tacggtagct
    12661 gtttgtgttg taactattat agttattgta gttatatgat tgattattat ttgagtagtt
    12721 gttgtaacgg ctgtagttat tgtagctata accgttgttg taattgttat agttattgta
    12781 accattgtag tagtaatagc tgtagtagcc attatcttgg tttaattgac ttggatgcca
    12841 gttacctttc catgtgtaat ggtagttacc ttgtgcatca atagtgtaag tatagctata
    12901 tgatgttggg tcgtttggat tataaccgta gttatcttgc tcagaagcat gagcttgatt
    12961 tcctgatgca attgcgattg tagcgaatcc tgcagttgcg atagtagctg tagcgatttt
    13021 cttcatttta aaaatatcct cctaaaaatt ttaaatctaa aatattttcg taatgtccgt
    13081 gtgacaaaat taatgttata agttatctct cgtaattaaa cgacaagaaa gactataaca
    13141 gaaattagcg tccttgtgtg ctttgttaac gttttgtaat tttttgctaa tatcttgaca
    13201 caatagaatt ttaaaagtat agaaatttgc attttgcaaa acttataact acggcattct
    13261 ttgtgaaaac tgaatgtttc gaaaataagt ctgttacaaa tttgtaatat tactgaaaat
    13321 tctaaatgta tattttgtgc ataatatagg acttttaatc agaaaatgca taaaaattcg
    13381 acacgaacga tcaagtttct atgattatga aagctttggt atttggatga aaatgtatat
    13441 ttaatggata aaatatccta atttagcata aaaaaatgtt ttaataaaag tattatttga
    13501 tataatcgat ttatgttttg ttactgctaa aaaacatgtg gcgatataat tggtagtgaa
    13561 tattagatgt tgacaattga tttgagaaag acaaacagga gtaaatcaat atatttgtat
    13621 atgtgaggat gtaagatgac agataattat tcactacata tttataaaag tatgaaaatg
    13681 agtagtgtga aggaagcgaa cttaaaaatt atatattggt tacaaggtac aggtgtagtt
    13741 tcaatcaatc tacaacaata tgatctaaaa agaaattatg tcaccattat tatgttgaat
    13801 gatttgtatc aaattgatgg taatgatgat agtgtatgtt gtgttgttga agtaagtgca
    13861 aagacatttt tgaagtttat gaatgcaaac tatatgatgc gtggcaagat attaacaaat
    13921 gaggttgcta gcacttttag aatcttgatt aagtatttaa tctacagcaa aattaaacaa
    13981 cagtcttata atgctaatga taagattatt aattacatgt gtgttgaatt aatgtcatta
    14041 aatacatacg atggtagtcg tcatttagta gccgaagaag tgcacgatta tttaacaaac
    14101 aaccaccata aaaagataaa taggaaagat gtcattaatc aagttagtat tacaaacaaa
    14161 gctttaagtg acatgtttaa agcgacacct tatagtaatt ttattcaata tttaaatcat
    14221 attagactgg agcattgctt aattgatatt ttaacttcta agaagcctat tgaagaaatc
    14281 gcaagtagtc atggttttaa tcattattct cgttttatac acctctttaa agaaacgtat
    14341 ggtaacactc ctaaattaat tagaaaaaga tatagtccaa catctcattc aattaaccat
    14401 tcccaattag ttgaaattga taaaaatata attgaattat ttgatgatac taatcaaagt
    14461 tcttcaagta tgcgcgagat tgatatacct tttgagccta caaaaaggag tcaaatgtat
    14521 caacctaaaa acatttat
//
LOCUS       DAMQYT010000051        14381 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMQYT010000051 DAMQYT010000000
VERSION     DAMQYT010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14381
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.051"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            64..6624
                     /gene="sasC"
                     /locus_tag="PGA33_001173"
     CDS             64..6624
                     /gene="sasC"
                     /locus_tag="PGA33_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HDG6472998.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDKDVANNRGLANSAQNTPNQSATTNQATNQALVNHNNGSIVNQAT
                     PTSVQSSTPSAQNNNHTDGNTTATETVSNANNNDVVSNNTALNVPTKTNENGSGGHLT
                     LKEIQEDVRHSSNKPELVAIAEPASNRPKKRSRRAAPADPNATPADPAAAAVGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNGIRPSTNRSVPTVNVVNNLPGFTLIN
                     GGKVGVFSHAMVRTSMFDSGDNKNYQAQGNVIALGRIHGTDTNDHGDFNGIEKALTVN
                     PNSELIFEFNTMTTKNGQGATNVIIKNADTNDTIAEKTVEGGPTLRLFKVPDNVRNLK
                     IQFVPKNDAITDARGIYQLKDGYKYYSFVDSIGLHSGSHVFVERRTMDPTATNNKEFT
                     VTTSLKNNGNSGASLDTNDFVYQVQLPEGVEYVNNSLTKDFPSNNSGVDVNDMNVTYD
                     AANRVITIKSTGGGTANSPARLMPDKILDLRYKLRVNNVPTPRTVTFNETLTYKTYTQ
                     DFINSAAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNGLKRRAQT
                     ILDENRNNVPLNKRVSQAYIDSLTNQMQHTLIRSVDAENAVNKKVDQMEDLVNQNDEL
                     TDEEKQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQREIINATPDVTEDEIQDALNQLATDETDAIDNVTNATTNADVETAKNNG
                     INTIGAVVPQVTHKKAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHAL
                     EQINQATTNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDAT
                     QEERQAAIDKVNAAVTAANTNILNANTNADVEQVKTNAIQGIQAITPATKVKTDAKNA
                     IDKSAETQHNTIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGT
                     QNIVVIQPATQVKTDTRNVVNDKAREAITNINATTGATREEKQEAINRVNTLKNRALT
                     DIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATT
                     EEKQVALNQVDQELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQI
                     NQHYNAKLAEINATPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAEN
                     NIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAINQ
                     INQNQTNDQVDTTTNQAVNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDN
                     EKEVASQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKIN
                     QKANELRAKINQDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDS
                     IALVTPDHIVRAAARDAVKQQYEAKKREIEQAEHATDEEKQVALNQLANNEKRALQNI
                     DQAIANNDVKRVETNGIATLKGVQPHIVIKPEAQQAIKASAENQVESIKDTPHATVDE
                     LDEANQLISDTLKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEE
                     NNKNQLDAIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNI
                     KVILPKVQVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQIN
                     HADKTAQVNQDSINAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQ
                     NIAIAQVEKELIKAKQQIASAVTNADVAYLLHDEKNEIREIEPVINRKASAREQLTTL
                     FNDKKQAIEANIQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIH
                     DLDVHPIKKPDAEKTINDDLARVTALVQNYRKVSNRNKADALKAITALKLQMDEELKT
                     ARTNADVDAVLKRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKV
                     KVLIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEATKVSPKEIQPAPKV
                     CTPIKKEETHESRKVEKELPNTGSEGMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(6741..8009)
                     /locus_tag="PGA33_001174"
     CDS             complement(6741..8009)
                     /locus_tag="PGA33_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDG6472999.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKHISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHASME"
     gene            8416..10077
                     /locus_tag="PGA33_001175"
     CDS             8416..10077
                     /locus_tag="PGA33_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDG6473000.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNIHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            10074..10769
                     /locus_tag="PGA33_001176"
     CDS             10074..10769
                     /locus_tag="PGA33_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDG6473001.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYIENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            10786..11208
                     /locus_tag="PGA33_001177"
     CDS             10786..11208
                     /locus_tag="PGA33_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDG6473002.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFKEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            11958..13367
                     /gene="sapep"
                     /locus_tag="PGA33_001178"
     CDS             11958..13367
                     /gene="sapep"
                     /locus_tag="PGA33_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HDG6473003.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            13371..14219
                     /gene="dat"
                     /locus_tag="PGA33_001179"
     CDS             13371..14219
                     /gene="dat"
                     /locus_tag="PGA33_001179"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HDG6473004.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     IESHSI"
BASE COUNT         5400 a         2241 c         2601 g         4139 t
ORIGIN      
        1 cgtatatttt tagcataatt cttaacaaca cagcagagaa cagacaacca ggaggaaaat
       61 gaaatgaatt tgttaaagaa aaataaatat agtattagga agtataaagt aggcatattc
      121 tctactttaa tcggaacagt tttattactt tcaaacccaa atggtgcaca agccttaact
      181 acggataata atgtacaaag cgatactaat caagcaacac ctgtaaattc acaagataaa
      241 gatgttgcta ataatagagg tttagcaaat agtgcgcaga atacacctaa tcaatctgca
      301 acaaccaatc aagcaacgaa tcaagcattg gttaatcata ataatggtag tatagtaaat
      361 caagctacgc caacatcagt gcaatcaagt acgccttcag cacaaaacaa taatcataca
      421 gatggcaata caacagcaac tgagacagtg tcaaacgcta ataataatga tgtagtgtcg
      481 aataataccg cattaaatgt accaactaaa acaaatgaaa atggttcagg aggacatcta
      541 actttaaagg aaattcaaga agatgttcgt cattcttcaa ataaaccaga gctagttgca
      601 attgctgaac cagcatctaa tagaccgaaa aagagaagta gacgtgcggc accggcagat
      661 cctaatgcaa ctccagcaga tccagcggct gcagcggtag gaaacggtgg tgcaccagtt
      721 gcaattacag cgccatatac gccaacaact gatcctaatg ccaataatgc aggacaaaat
      781 gcacctaacg aagtgctgtc atttgatgac aatggtatta gaccaagtac caaccgttct
      841 gtgccaacag taaacgttgt taataacttg ccgggcttca cactaatcaa tggtggcaaa
      901 gtaggggtgt ttagtcatgc aatggtaaga acgagcatgt ttgattcagg agataataag
      961 aactatcaag cacaaggaaa tgtaattgca ttaggtcgta tacatggaac tgatacgaat
     1021 gaccatggcg attttaatgg tatcgagaaa gcattaacag taaatccgaa ttctgaatta
     1081 atctttgaat ttaatacaat gactactaaa aacggtcaag gcgcaacaaa tgttattatt
     1141 aaaaatgctg atactaatga tacgattgct gaaaagactg ttgaaggcgg tccaactttg
     1201 cgtttattta aagtacctga taatgtgaga aatctcaaaa ttcaatttgt acctaaaaat
     1261 gacgcaataa cagatgcgcg tggcatttat caactaaaag atggttacaa atactatagc
     1321 tttgttgact ctatcggact tcattctggg tcacatgttt ttgttgaaag acgaacaatg
     1381 gatccaacag caacaaataa taaagagttt actgtaacaa catcattaaa gaataatggt
     1441 aattctggtg cttctctaga tacaaatgac tttgtatatc aagttcaatt acctgaaggt
     1501 gttgaatatg tgaacaattc attgactaaa gattttccaa gtaacaattc aggcgttgat
     1561 gttaatgata tgaatgttac atatgatgca gcaaatcgtg tgataacaat taaaagtact
     1621 ggaggaggta cagcaaactc tccggcacga cttatgcctg ataaaatact cgatttaaga
     1681 tataaattac gtgtaaataa tgtgccgaca ccaagaacag taacatttaa cgagacatta
     1741 acgtataaaa catatacaca agatttcatt aattcagctg cagaaagtca tactgtaagt
     1801 acaaatccat atactatcga tatcatcatg aataaagatg cattacaagc cgaagttgac
     1861 agacgtattc aacaagctga ttatacattt gcgtcattag atatctttaa tggtctgaaa
     1921 cgacgcgcac aaacgatttt agatgaaaat cgtaacaatg taccattaaa taaaagagtt
     1981 tctcaagcat atattgattc attaactaat caaatgcaac atacgttaat tcgaagtgtt
     2041 gatgctgaaa atgcagttaa taaaaaagtt gaccaaatgg aagatttagt taatcaaaat
     2101 gatgaattga cagatgaaga aaaacaagca gcaatacaag ttatcgagga acataaaaat
     2161 gaaataattg gtaatattgg tgaccaaacg actgatgatg gcgttactag aatcaaagat
     2221 caaggtatac agaccttaag tggggatact gcaacaccgg ttgttaaacc aaatgctaaa
     2281 aaagcaatac gtgataaagc aacgaaacaa agggaaatta tcaatgcaac accagatgtt
     2341 actgaagacg agattcaaga tgcactaaat caattagcta cggatgaaac agatgctatt
     2401 gataatgtta cgaatgctac tacaaatgct gacgttgaaa cagctaaaaa taatggcatc
     2461 aatactattg gagcagttgt tcctcaagta actcataaaa aagctgcaag agatgcaatt
     2521 aaccaagcaa cagcaacgaa aagacaacaa ataaatagta atagagaagc aactcaggaa
     2581 gagaaaaatg cagcattgaa cgaattaact caagcaacca accatgcttt agaacaaatc
     2641 aatcaagcaa caacaaatgc tgatgttgat aacgccaaag gagatggtct aaatgccatt
     2701 aatccaattg ctcctgtaac tgttgttaag caagctgcaa gggatgccgt atcacatgat
     2761 gcacaacaac atatcgcaga gatcaatgct aatcctgatg cgactcaaga agaaagacaa
     2821 gcagcaattg acaaagtgaa tgctgctgta actgcagcaa acacaaacat tttaaacgct
     2881 aataccaatg ctgatgttga acaagtaaag acaaatgcga ttcaaggaat acaagcaatt
     2941 acaccagcta caaaagtaaa aacagatgca aaaaatgcca tcgataaaag tgcggaaacg
     3001 caacataata cgatatttaa taataatgat gcgacgctcg aagaacaaca agcagcacaa
     3061 caattacttg atcaagctgt agccacagcg aagcaaaata ttaatgcagc agatacgaat
     3121 caagaagttg cacaagcaaa agatcagggc acacaaaata tagtagtgat tcaaccggca
     3181 acacaagtta aaacggatac tcgcaatgtt gtaaatgata aagcgcgaga ggcgataaca
     3241 aatatcaatg ctacaactgg cgcgactcga gaagagaaac aagaagcgat aaatcgtgtc
     3301 aatacactta aaaatagagc attaactgat attggtgtga cgtctactac tgcgatggtc
     3361 aatagtatta gagacgatgc agtcaatcaa atcggcgcag ttcaaccgca tgtaacgaag
     3421 aaacaaactg ctacaggtgt attaaatgat ttagcaactg ctaaaaagca agaaattaat
     3481 caaaacacaa atgcaacaac tgaagaaaag caagtggctt taaatcaagt ggatcaagag
     3541 ttagcaacgg caattaataa tataaatcaa gctgatacaa atgcggaagt agatcaagcg
     3601 caacaattag gtacaaaagc aattaatgcg attcagccaa atattgttaa aaaacctgca
     3661 gcattagcac aaatcaatca gcattataat gctaaattag ctgaaatcaa tgctacacca
     3721 gatgcaacga atgatgagaa aaatgctgcg atcaatactt taaatcaaga cagacaacaa
     3781 gctattgaaa gtattaaaca agctaacaca aatgcagaag tagaccaagc tgcgacagta
     3841 gcagagaata atatcgatgc tgttcaagtt gatgtagtaa aaaaacaagc agcgcgagat
     3901 aaaatcactg ctgaagtggc gaagcgtatt gaagcggtta aacaaacacc taatgcaact
     3961 gacgaagaaa agcaggctgc tgttaatcaa atcaatcaac ttaaagatca agcaattaat
     4021 caaattaatc aaaaccaaac aaatgatcag gtagacacaa ctacaaatca agcggtaaat
     4081 gctatagata atgttgaagc tgaagtagta attaaaccaa aggcaattgc agatattgaa
     4141 aaagctgtta aagaaaagca acagcaaatt gataatagtc ttgattcaac agataatgag
     4201 aaagaagttg cttcacaagc attagctaaa gaaaaagaaa aagcacttgc agctattgac
     4261 caagctcaaa cgaatagtca ggtgaatcaa gcagcaacaa atggtgtatc agcgattaaa
     4321 attattcaac ctgaaacaaa agttaaacca gctgcacgtg aaaaaatcaa tcaaaaagcg
     4381 aatgaattac gtgctaagat taatcaggat aaagaagcaa cagcagaaga aagacaagta
     4441 gcactagata aaatcaatga atttgtaaat caagccatga cagatattac gaataataga
     4501 acaaatcaac aagttgatga tacaacaagt caagcgcttg atagcattgc tttagtgacg
     4561 cctgaccata ttgttagagc agctgctaga gatgcagtta agcaacaata tgaagctaaa
     4621 aagcgcgaaa ttgagcaagc ggaacatgcg actgatgaag aaaaacaagt tgctttaaat
     4681 caattagcga ataatgaaaa acgtgcatta caaaacatcg atcaagcaat agcgaataat
     4741 gatgtgaaac gtgttgaaac aaatggcatt gctacactaa aaggtgtaca acctcatatt
     4801 gtaattaagc ctgaagcaca acaagcaata aaagcaagtg cagaaaatca agtagaatca
     4861 ataaaagata caccacatgc aacagttgat gaattagatg aagcgaatca attaattagc
     4921 gacacactca aacaagcgca acaagaaata gaaaatacaa atcaagatgc tgctgttact
     4981 gatgttagaa atcaaacaat caaggcaata gagcaaataa aacctaaagt aagacgtaaa
     5041 cgagctgcgc ttgatagcat tgaagaaaat aataaaaatc aactcgatgc aatccgaaat
     5101 acgttggata ctactcaaga tgaaagagat gttgctattg atactttaaa taaaattgta
     5161 aatacaatta aaaatgacat tgcacaaaac aaaacgaatg cagaagtgga tcgaactgag
     5221 actgatggca acgacaacat caaagtgatt ttacctaaag ttcaagttaa accagcagcg
     5281 cgtcaatctg ttggtgtaaa agccgaagct caaaatgcac taatcgatca aagcgattta
     5341 tcaactgaag aagaaagact agctgctaaa catttagtag aacaagcact taatcaggct
     5401 attgatcaga tcaatcatgc agataagact gcccaagtta atcaagatag tataaatgct
     5461 caaaatatta tttcaaaaat taaaccagcg acaacagtta aagcaacagc attacaacaa
     5521 attcaaaata tcgctacaaa taaaattaat ttaattaaag caaataacga agcgacagat
     5581 gaagaacaaa atattgcaat agcacaagtt gaaaaagagt taattaaagc taaacaacaa
     5641 attgctagtg cagtgactaa tgcagatgtg gcatatttat tgcatgatga gaaaaacgaa
     5701 attcgtgaaa tcgaacctgt tattaacaga aaggcgtctg ctcgagaaca attgacaaca
     5761 ttattcaacg ataaaaaaca agcaattgaa gcgaatattc aagcaacggt agaagaaaga
     5821 aatagtatat tagcacagtt acaaaatatt tatgacactg ctattggaca aattgatcaa
     5881 gatcgtagca atgcacaagt tgataaaaca gcatcattaa atctacaaac aatacatgat
     5941 ttagatgtac atcctattaa aaagccagat gctgaaaaaa cgattaatga tgatcttgca
     6001 cgcgtcactg ctttagtgca aaattatcga aaagtaagta atcgtaataa ggctgatgca
     6061 ttaaaagcta taactgcttt aaaattacaa atggatgaag aattaaaaac agcacgcact
     6121 aatgctgatg ttgatgcagt tttaaaacga tttaatgttg cattaagcga tatagaagca
     6181 gtaattactg aaaaagaaaa tagcttactg cgaattgata acattgctca acaaacatat
     6241 gcgaaattca aagcgatcgc aacaccagaa caattagcta aagtaaaagt attaattgat
     6301 caatatgttg cagatggcaa tagaatgatt gatgaagatg cgacattaaa tgacatcaaa
     6361 caacacacgc aattcattgt tgatgaaatt ttagcaatta aattaccagc tgaagcgacg
     6421 aaagtatcac caaaagaaat tcagccagct ccaaaagttt gtacgcctat taaaaaagaa
     6481 gagacacatg aatcgcgcaa agttgaaaaa gaacttccaa atacaggttc tgaaggaatg
     6541 gatttaccat tgaaagaatt tgcactgatt acaggtgcgg ctttgttagc tagaagacgt
     6601 actaaaaacg aaaaagaatc ataattaacg aaatgacctt aatataacga cactttttag
     6661 tgactcagtt atattaaggt catttttatt tgaatgaaaa agttgatcta ctaaagagaa
     6721 tcgaaccaaa ttttatacta ttattccatt gatgcatgtg agtaatgtcc ggcatataat
     6781 ccagcgacat gtcctgttac gagtgcactt gtaatattat aaccaccagt ataaccatgt
     6841 atatctaata cttcaccaca taaaaataat cccggaacta atttagacat cattgtttta
     6901 ggttgaattt ctttaagtga cacaccacca cctgtgacaa atgccttatc tataggtaat
     6961 gtcccgttca ccttaaatac aaacccttta aacatattta ctaagtcgtt caattgttga
     7021 tttgataagt gatgtgatgt ggtattttca tcgattcctg cttgttccag catgaacagt
     7081 aagtagcgct cttcaattag accatgcaaa ctgtttttaa tgattttatc tggtgtgtcc
     7141 gataataatg atgtgatgtg ttgttttaat tgttcatggt ttaattcagg aaatgcatcg
     7201 attgccatag aaatgtgctg tgtcttttga tttttttgtt ctttataaac aaactgacta
     7261 catcttaatg cagctggacc actgatacca aaatgagtaa ataacatatc catttgatga
     7321 ctgatgtgtt ttttaccatt tttcttaagt actgacaatt caacatcttt taaacttaaa
     7381 ccttttagac gattggattt gatgaaaggt tcagctgatg taattggaac ttcggtcggg
     7441 aataactcag taatggtatg acctaaatct tgtgcgaact tataaccatc accagttgaa
     7501 ccagtttgag ggacacttgt accacctgta gcaatcacta gcgaatggct ttcataacta
     7561 ttattttgag tatgtacagt gaaagtttgg tctgtattaa cttcgattct actaacagct
     7621 tcttcttctt taatcgttac atgttggcgt tcgatagttg tcactaatgt atcaaccacg
     7681 tcttgtgctt tgttggaaac tggaaacata cgcccgtgat cttcttcttt taatttaaca
     7741 cccctagact caaaaaaatc tatgatggat tcattatcaa aaattgaaaa gggactatat
     7801 aaaaatttcc catttccagg aatgttctta ataatttcag catatggtaa tcgattagtt
     7861 acgttacatc taccgccacc agatattttg agtttacgac ctagaccttt ctttttttca
     7921 atgagtaaca cactgctact ttgttcgctt gcagctactg ccgccattaa gccgctaggt
     7981 ccgcctccga taataattgt ttgatacata tacgcttcct ccaaatttgt tactggacat
     8041 tcaattgcta ttttatagta cttttttaaa ctttgctata tctcttgtgt tatgagatga
     8101 caattatgtt gaatatattt ataatgtgaa cttcataaaa aatgaagatg tatgtgtatg
     8161 ataagaatga tttttctact gttatgcgat gttcatctca aatagtagta taatgtttat
     8221 atgaaatttc gtaatattct aatcaaaatc tagtattcaa tgacaatgtg ttgataattt
     8281 tgttatgata attcattgaa tatacgacaa tgaaataaag ttttgtgata tattcataat
     8341 aatgaaaaat gattttacag agaaacagct tttagtttac aaaactttaa taaacatgag
     8401 atagggagat tcgtaatgag tgaaagtaaa gaaatggtgc gtggaacctt tttaattacc
     8461 attagtatat taattaccaa ggtattgggc gtacttttta tcattccatt caactattta
     8521 attggtggac aagaaaatat ggcgccgttc acatacgctt atgcaccata taatattgca
     8581 attgcagttg cgacagcagg tgtaccatta gcagcttcga aatatgttgc gaaatataat
     8641 gctattgggg catataaagt aagtcagaaa ttttataaat cgagttttat tgtaatgagt
     8701 attaccggtg ttttaggttt cttggtactg tatttcttag caccttatat ttctgaatta
     8761 acattagcta gaaacattca tgataaaaac ggttggtcag tcgatgatat tacttggatt
     8821 ataagaatca ttagtatggt tgttatcttt attcctgtat tagcgacatg gagagggata
     8881 ttccaaggtt ataaatctat ggggccaaca gcagtgtcag aagtaactga gcaaattgca
     8941 cgcgtgattt tcatattaat cggaagttat ttagtactaa atgtctttga tggctctatt
     9001 ttattagcca atggtattgc tacttttgca gcagcagttg gtgcgattat tggtatattc
     9061 acactctggt attactggag aaagcgtaag cataatattg atcgtatggt cgaatctgat
     9121 tacacagata tagatgtgtc atatggcaag atgtataaag aaattattgc ttatagtata
     9181 ccgttcgtta ttgtaagttt gaattatcca ttatttaact tagtagatca atttacacat
     9241 aacggtgctt tatctttagt tggtatacct tcacagttac aagatatatt ctttaatatg
     9301 ctaaatatgt caactaataa aattgtaatg attccgacat cattaagtgc tggttttgca
     9361 gtgagtttaa ttccttatat tacaaaaaca tttgcagaag gtcgcttaca tgaaatgcat
     9421 catcaaatta gaacatctat tggtgtatta atgtttataa ctgttccagc tagtattgga
     9481 attatggcct tggcacaacc attattcaca gttttctatg gttacgatcc aattgtttta
     9541 ggtcatgacc ctaaccatga tggtagtcgc ttattattct attatgcgcc tgtagcaata
     9601 ctaatttcac tattaagtgt aacggcatca atgttacaag gtatagataa acagaaatta
     9661 acagtttatg ttattttagc ttctgtagtt atcaagttag cgttaaacta tccattaatt
     9721 atgttattcc atacacctgg tgcaatttta agtactagta tcgcattatt atttgcaatt
     9781 ggatgtaatt tctatattct taaaaaatat gcgaagttta aattcagcta tagttggatt
     9841 cattttgcaa aaatattctt gtattcattt attatgatgc taggtgtaga attagtattt
     9901 ttccttgcaa atctattctt agaacctact aaacttggtt acttaatcat tattatttta
     9961 ggtgttacag taggtattct aatttacggt acaatcacaa ttaaaacgcg tcttgcggac
    10021 gaattcttag gtgaaattcc tgaaaaatta agacgtagag ttaggttttt acgatgagaa
    10081 tagataaatt tttagcaaat atgggcgtcg gtacacgtaa tgaggtcaaa cagttactta
    10141 aaaaaggcct tgtaaatgtc aacgaacaag taattaaatc accaaaaaca catatagaac
    10201 cagaaaacga taagataaca gttcgtggag aattaataga atacattgaa aatgtctata
    10261 ttatgttaaa taagccaaaa ggttatatat cagcaactga agatcatcat tcaaaaaccg
    10321 ttattgattt aatacctgaa taccaacatc taaatatttt tccggttggt cgtcttgata
    10381 aagatacaga agggctttta ttgataacga atgatggtga ttttaatcat gaattaatga
    10441 gtccaaacaa acatgtttct aaaaaatatg aagttatttc agcaaatcct atcactgaag
    10501 acgacattca agcttttaaa gagggtgtaa cactgactga tggcaaggtt aagcctgcaa
    10561 tattaactta tattgataat caaacgtcac acgtaacaat ttatgaagga aaatatcatc
    10621 aagttaaacg aatgtttcat agcattcaaa atgaggtatt acatttaaga cgtatcaaaa
    10681 ttgctgattt agaattggac agcaatttag attcaggaga atatcgttta ttaactgaaa
    10741 atgattttga caaattaaat tataaataaa agggagacga ttgatatggc aaaaggtaat
    10801 ttatttaaag cgattttagg tataggtggc gctgtagcag ctgtacttgt tacacgtaaa
    10861 gatagtcgtg acaagctgaa agcagaatat aataaatata aacaagatcc tcaaagctat
    10921 aaagataatg ctaaggataa agcgacgcaa ttaggaacaa ttgctaatga aacaattaaa
    10981 gaagtaaaaa caaatccgaa agaatatgct aatagattaa aaaataatcc aaaagcattt
    11041 ttcaaagaag aaaaatcaaa atttaccgaa tatgacaata agactgacga aagtattgaa
    11101 aaaggtaaat ttgatgatga aggtggcgca gcaccaaata ataatttacg tatcgtcact
    11161 gaagaagatt taaaaaagaa taaaaatgca ttatctgata aagaataaga tgtgagtagg
    11221 acagaaatga taagggagtg ggacagaaat catattttag caaaatttat ttcgtcgtcc
    11281 caccccggca aggttgacta gaattgaaaa aagcttgtta caagcgtatt ttcattcagt
    11341 caactactgc caatataata ttgtaaacta taggacattt attagtgttt caggttcttt
    11401 atcgttagtc atagctaata tgcatataga aagaagaata catgcgtgaa actcattcat
    11461 aagaaatact aatatctaaa gaaaacgtat ttctttatct tttcgaaatt ctctgtgttg
    11521 gggcccttac cccaacttgc acattattga aagctgacta ttggccagct tctgtgttgg
    11581 ggcccttacc ccaacttgca cattattgaa agctaactat tggccagctt ctatgttggg
    11641 gccccgccaa cttgcattgt ctgtagaatt tcttttcgaa attctctatg ttggggcccc
    11701 ggggcgcatt ttcgttcggt taattactgt caatattaaa ttgtgtgcct agtacattga
    11761 cttatatctc gagctcttga atatgtgtat tttaaatata taaattaaaa aaggaacaaa
    11821 gcagctattt atgatttgtg tcatatcaat agcatcgttt tgttcctttt tattattttc
    11881 tgacatctta ttgtagtttt tatatctttt catgtaaaat agaatttaac ttatagaaaa
    11941 aaggaagtcg atttcgaatg tggaaagaaa aagttcaaca atacgaagat caaatcatta
    12001 atgacttaaa aggattatta gcaattgaaa gtgtgagaga tgatgcaaaa gcatcagaag
    12061 acgcaccagt tggtccaggt cctcgtaagg cattagacta catgtatgaa attgcacata
    12121 gagatggatt tacaacacat gatgtggatc atattgcagg aagaattgag gcaggtaaag
    12181 gaaatgacgt attaggtatc ttatgtcatg ttgacgttgt tcctgctggt gatggatggg
    12241 atagtaatcc gttcgagccg gttgtaacag aagatgctat catagctaga ggtacacttg
    12301 atgacaaagg tccaacaatt gctgcttatt atgcaattaa gatattagaa gatatgaatg
    12361 tggattggaa gaaacgtatt catatgatta ttggtacgga tgaagaatct gattggaaat
    12421 gtacggatcg ctattttaaa acagaagaaa tgccaacatt aggttttgca ccagatgcag
    12481 aatttccatg tattcatggt gaaaaaggca ttacaacatt tgatttagtt caaaataaac
    12541 ttactgaaga tcaagatgaa cctgattatg aattaataac ttttaaatct ggtgaacgtt
    12601 acaacatggt acctgatcat gcagaagcaa gagtgcttgt taaagaaaat atgacagatg
    12661 ttattcaaga ctttgagtac tttttagaac aaaatcattt acaaggtgat agtactgttg
    12721 atagtggcat tctagtttta acagttgaag gtaaagcggt tcatggtatg gatccatcta
    12781 tcggtgtgaa tgcgggtctt tacttactaa aattcttagc atcattaaat cttgataata
    12841 atgcacaagc gtttgtagca tttagtaatc gctacttatt taattcagat tttggtgaaa
    12901 agatgggaat gaaattccat acagatgtca tgggtgacgt gacaactaac attggtgtta
    12961 ttacatatga taatgaaaac gcaggtcttt tcggtatcaa cttacgctac ccagaaggat
    13021 ttgaatttga aaaagctatg gatcgttttg caaatgagat tcaacaatat ggctttgaag
    13081 tgaaattagg taaagtccaa ccaccacatt atgttgataa aaatgatcct tttgtacaaa
    13141 agttagttac tgcatataga aatcaaacaa atgatatgac tgaaccttat actataggtg
    13201 gcggtactta tgcgagaaac ttagacaagg gtgtagcatt tggcgcaatg tttagtgatt
    13261 ctgaagattt aatgcatcag aaaaatgaat atatcactaa aaaacagtta tttaacgcaa
    13321 ctagtattta cttagaagca atttattcat tatgcgtgga ggaataatat atggaaaaaa
    13381 tttttttaaa tggtgagttt gtaagtccaa gtgaagcaaa ggtttcatac aacgacagag
    13441 gatacgtatt tggcgatggt atttatgaat acattcgagt atataatggt aagttattta
    13501 cagtaacaga acattatgaa agatttttac gtagtgccaa tgagattggt ttagatttaa
    13561 attattctgt agaagaatta attgaactat ctcgtaaatt agttgatatg aatcaaattg
    13621 aaactggggc aatttatatt caagcaacgc gtggtgtagc tgaaaggaat catagcttcc
    13681 cgacacctga agtagaacca gcaattgttg cttatacaaa gagttatgat cgtccttatg
    13741 atcatttaga aaatggtgtg aatggtgtta ccgttgaaga tatccgatgg ttacgttgcg
    13801 acattaaaag cttgaactta ttaggaaatg tattagcaaa agaatatgct gtgaaatata
    13861 atgcagttga agcaattcaa catcgaggtg aaactgtaac tgaaggatct tcaagtaatg
    13921 cttatgcaat taaagacggt gtgatttata cacatccgat taacaactat attcttaatg
    13981 gtattacacg aattgtaatt aaaaaaattg ccgaagacta taacatccca tttaaagaag
    14041 aaacgtttac tgtagatttc ttgaaaaacg cagatgaagt tattgtttca agtacttcag
    14101 ctgaggttac acctgttatt aaattagatg gtgaaccagt taatgatggt aaagttggcc
    14161 caattacacg tcaactacaa gaaggatttg aaaagtatat agagtcacac agtatttaaa
    14221 attctttcat catattttta gattaaatta cttaaattta tcaactttcg taagtgaatt
    14281 atgttataat ttaaaacgaa gcttaaaagt gttgtttaga aaacacatat tagtaaaaag
    14341 tgccattttg tttctcattt tcagttgaaa tataatgata a
//
LOCUS       DAMQYT010000052        14223 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMQYT010000052 DAMQYT010000000
VERSION     DAMQYT010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14223
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.052"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            294..1775
                     /locus_tag="PGA33_001180"
     CDS             294..1775
                     /locus_tag="PGA33_001180"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDG6473005.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVD"
     gene            1759..2019
                     /locus_tag="PGA33_001181"
     CDS             1759..2019
                     /locus_tag="PGA33_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HDG6473006.1"
                     /translation="MALLINKRFIDEGKTIDVYLFEALNNQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            2019..3581
                     /locus_tag="PGA33_001182"
     CDS             2019..3581
                     /locus_tag="PGA33_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDG6473007.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
     gene            3883..4686
                     /locus_tag="PGA33_001183"
     CDS             3883..4686
                     /locus_tag="PGA33_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDG6473008.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            4905..7229
                     /locus_tag="PGA33_001184"
     CDS             4905..7229
                     /locus_tag="PGA33_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDG6473009.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDSVDQDTVKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHIPSISDDKDNVMRENHIVDDNPDNDINTPSLSKTDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            7246..8604
                     /locus_tag="PGA33_001185"
     CDS             7246..8604
                     /locus_tag="PGA33_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDG6473010.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMILMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            8743..10254
                     /locus_tag="PGA33_001186"
     CDS             8743..10254
                     /locus_tag="PGA33_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDG6473011.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSDNFIVKNR"
     gene            complement(10434..10522)
                     /locus_tag="PGA33_001187"
     tRNA            complement(10434..10522)
                     /locus_tag="PGA33_001187"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10485..10487),aa:Ser,seq:gct)
     gene            complement(10525..10599)
                     /locus_tag="PGA33_001188"
     tRNA            complement(10525..10599)
                     /locus_tag="PGA33_001188"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10565..10567),aa:Asn,seq:gtt)
     gene            complement(10741..11310)
                     /locus_tag="PGA33_001189"
     CDS             complement(10741..11310)
                     /locus_tag="PGA33_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HDG6473012.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
     gene            11520..11738
                     /locus_tag="PGA33_001190"
     CDS             11520..11738
                     /locus_tag="PGA33_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HDG6473013.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
     gene            complement(11819..12805)
                     /locus_tag="PGA33_001191"
     CDS             complement(11819..12805)
                     /locus_tag="PGA33_001191"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDG6473014.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            13004..13180
                     /locus_tag="PGA33_001192"
     CDS             13004..13180
                     /locus_tag="PGA33_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HDG6473015.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            13195..13797
                     /locus_tag="PGA33_001193"
     CDS             13195..13797
                     /locus_tag="PGA33_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDG6473016.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLSSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            14030..14175
                     /locus_tag="PGA33_001194"
                     /pseudo
     CDS             14030..14175
                     /locus_tag="PGA33_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
BASE COUNT         5022 a         1940 c         2576 g         4685 t
ORIGIN      
        1 attttataaa gcaatttatt gttctatgtt ttatttgtat atttaaaatt atccagtata
       61 caattatagc atatttttgg aaacaattat gatattatac catgttacaa gatggtttta
      121 ataatttaag atgagccata attgtaaaac taattcataa taccgtatgt tttattttta
      181 atagtagaaa ttagaaaatg ctgattagta ggatataaca gtgaaattat aaatttatta
      241 acatcaacaa aacgtgtata ataaacatat tgtagaaaaa ggagcggttc agtttggatg
      301 caagtacgtt gtttaagaaa gtaaaagtaa agcgtgtatt gggttcttta gaacaacaaa
      361 tagatgatat cactactgat tcacgtacag cgagagaagg tagcattttt gtcgcttcag
      421 ttggatatac tgtagacagt cataagttct gtcaaaatgt agctgatcaa gggtgtaagt
      481 tggtagtggt caataaagaa caatcattac cagctaacgt aacacaagtg gttgtgccgg
      541 acacattaag agtagctagt attctagcac acacattata tgattatccg agtcatcagt
      601 tagtgacatt tggtgtaacg ggtacaaatg gtaaaacttc tattgcgacg atgattcatt
      661 taattcaaag aaagttacaa aaaaatagtg catatttagg aactaatggt ttccaaatta
      721 atgaaacaaa gacaaaaggt gcaaatacga caccagaaac agtttcttta actaagaaaa
      781 ttaaagaagc agttgatgca ggcgctgaat ctatgacatt agaagtatca agccatggct
      841 tagtattagg acgactgcga ggcgttgaat ttgacgttgc aatattttca aatttaacac
      901 aagaccattt agattttcat ggcacaatgg aagcatacgg acacgcgaag tctttattgt
      961 ttagtcaatt aggtgaagat ttgtcgaaag aaaagtatgt cgtgttaaac aatgacgatt
     1021 cattttctga gtatttaaga acagtgacgc cttatgaagt atttagttat ggaattgatg
     1081 aggaagccca atttatggct aaaaatattc aagaatcttt acaaggtgtc agctttgatt
     1141 ttgtaacgcc ttttggaact tacccagtaa aatcgcctta tgttggtaag tttaatattt
     1201 ctaatattat ggcggcaatg attgcggtgt ggagtaaagg tacatcttta gaaacgatta
     1261 ttaaagctgt tgaaaattta gaacctgttg aagggcgatt agaagtttta gatccttcgt
     1321 tacctattga tttaattatc gattatgcac atacagctga tggtatgaac aaattaatcg
     1381 atgcagtaca gccttttgta aagcaaaagt tgatattttt agttggtatg gcaggcgaac
     1441 gtgatttaac taaaacgcct gaaatggggc gagttgcctg tcgtgcagat tatgtcattt
     1501 tcacaccgga taatccggca aatgatgacc cgaaaatgtt aacggcagaa ttagccaaag
     1561 gtgcaacaca tcaaaactat attgaatttg atgatcgtgc agaagggata aaacatgcaa
     1621 ttgacatagc tgagcctggg gatactgtcg ttttagcatc aaaaggaaga gaaccatatc
     1681 aaatcatgcc agggcatatt aaggtgccac atcgagatga tttaattggc cttgaagcag
     1741 cttacaaaaa gttcggtggt ggccctgttg attaataaaa gatttattga tgaaggtaaa
     1801 actattgatg tttatttatt cgaagcatta aataaccaga taatcattgc tataccagat
     1861 tggttttggt catatcagat ggcaatgaca ttagatgaag aaacttgttt tgaagcaata
     1921 ctcatgcaat tgtttgtttt taaagaagag gaagaggcag aatcgattgc atcacaacta
     1981 acagattgga tagaaacata taaaaaggag aaagactaat gaacttaaag caagaagttg
     2041 agtctagaaa gacttttgcg attatttcac atcccgatgc agggaaaaca acgttaactg
     2101 aaaaactatt gtacttcagt ggtgctattc gtgaagcggg tacagttaaa gggaagaaga
     2161 ctggtaaatt tgcgacaagt gactggatga aagttgaaca agagcgtggt atttctgtaa
     2221 ctagttcagt aatgcaattt gattacgatg attataaaat caatatctta gatacaccag
     2281 gacatgaaga cttttcagaa gatacgtata gaacattaat ggcagttgac agtgctgtca
     2341 tggtcataga ctgtgcaaaa ggtattgaac cacaaacatt gaagttattt aaagtttgta
     2401 aaatgcgtgg tattccaatc tttacattca ttaataaatt agaccgagta ggtaaagaac
     2461 catttgaatt attagatgaa atcgaagaga cattaaatat tgaaacatac cctatgaatt
     2521 ggccaattgg tatgggacaa agtttctttg gcatcattga tagaaagtct aaaacaattg
     2581 aaccatttag agatgaagaa aatatattac atttgaatga tgattttgag ttggaagaag
     2641 atcatgcaat tacaaatgat agtgattttg aacaagcgat tgaagaatta atgttggttg
     2701 aagaagcggg tgaagccttt gataatgacg cgctgttgag tggagactta acacctgtat
     2761 ttttcggttc agctttagct aactttggtg tacaaaattt cttaaatgca tatgttgatt
     2821 ttgcgccaat gccaaatgcg agacaaacaa aagaagacgt tgaagtaagc ccgtttgatg
     2881 attcattttc aggatttatc tttaaaattc aagccaacat ggaccctaaa caccgtgata
     2941 gaattgcctt tatgcgtgtc gttagtggtg catttgaacg tggtatggat gttactttgc
     3001 aacgtactaa taaaaagcaa aagatcacac gttcaacgtc atttatggca gacgataaag
     3061 aaactgtgaa tcatgctgta gcaggcgata tcattggact atatgatact ggtaattatc
     3121 aaattggaga tactttagtt ggtggaaaac aaacctacag tttccaagat ttaccacaat
     3181 ttacgccaga aatttttatg aaagtttctg ctaaaaacgt catgaaacag aagcatttcc
     3241 ataaaggtat tgaacaatta gtacaagaag gtgcgattca atactataaa acattacaca
     3301 caaaccaaat tattttaggt gctgttggtc agttacaatt tgaagttttc gaacatagaa
     3361 tgaaaaacga atataatgtt gatgttgtta tggagccagt aggccgtaaa attgcacgtt
     3421 ggattgaaaa tgaagaccaa attacagata agatgaacac atcaagatcg attttagtga
     3481 aagatagata tgacgattta gtattcttat ttgaaaatga atttgcaaca agatggtttg
     3541 aagagaaatt ccctgaaatt aaattgtata gtttacttta acagctcaat tgtataatcg
     3601 aatttgttac attaaaaata attgtttcgt tgaagaaaaa taaattgtat attttaaaag
     3661 aaaaaggtat actatgatgt atcaaatgaa taacctatgg cattttgtca gaggggagta
     3721 acttaagaat catgaccgta taaatgattc gacactttat cgtcattacg aagatatctt
     3781 ccggtaaagt gggcaattta aattgcttag tgagaccttt gctatttatt tagcataggt
     3841 ctttttgttt gtacttaact tatttattta aaggagttgt acatgttaat ggatccaagt
     3901 ttgatcttac cttatttatg ggtacttgtc gttttagtat ttttagaagg cttattagca
     3961 gcagataacg cgattgttat ggctgtaatg gttaagcact taccacccga acaacgtaaa
     4021 aaagctttgt tttacggttt gttaggtgca tttgtattta gatttttagc attattctta
     4081 attagtatta tcgcgaactt ttggtttatt caagctgcag gagcggttta cttaatttat
     4141 atgtcaatca aaaatctgtg gcagttcttt aaacacccag aaattgaaag tcctgaagct
     4201 ggagatgatc atcattatga tgaatctggt gaagagatta aagcaagtaa caaatcattc
     4261 tggggaactg tgttgaaaat agaatttgca gatatcgcat ttgccattga ttctatgctt
     4321 gctgctttag ctattgctgt aacacttcct aaagttggta ttcactttgg tggtatggac
     4381 ttaggtcagt tcgtagtcat gttcctaggt ggaatgattg gtgttattct aatgcgttat
     4441 gcagcaacat ggtttgtaga gctattaaac aaatatccag gacttgaagg tgcagccttc
     4501 gcgatcgttg gttgggtagg tgttaaatta gttgtcatgg tattagcgca cccagacatc
     4561 gctgtattgc ctgagcactt cccacatggc gtattatggc aatctatttt ctggacagta
     4621 ctaattggat tagtaattat cggttggtta ggttcagttg ttaaaaataa aaaatcgcat
     4681 aaataattga tgtgaagcgg acaatcttaa tttagtttaa ggttgtcctt tttcatttaa
     4741 ttgagtgatt tatgaaaaat ggattttgaa gaatgtgaat caaaagatgc gatatagtat
     4801 taagaaaatg tgccttttat atttagcatt ttttcaatag aaattatata gattttaaag
     4861 caaattaggt gttaatgtgt cataatgata agtgatttta ttgaatggag tggacattag
     4921 tggatattgg taaaaaacat gtaattccta aaagtcagta ccgacgtaag cgtcgtgaat
     4981 tcttccacaa cgaagacaga gaagaaaatt taaatcaaca tcaagataaa caaaatatag
     5041 ataatacaac atcaaaaaaa gcagataagc aaatacataa agattcaatt gataagcacg
     5101 aacgttttaa aaatagttta tcatcgcatt tagaacagag aaaccgtgat gttaatgaga
     5161 ataaagctga agaaagtaaa agtaatcagg atagtaagtc agcatataac agagatcatt
     5221 atttaacaga cgatgtatct aaaaaacaaa attcattaga ttcagtggac caagatacag
     5281 tgaaatcaaa atattatgag caaaattctg aagcgacttt atcaactaaa tcaaccgata
     5341 aagtagaatc aactgaaatg agaaagctaa gttcagataa aaacaaagtt ggtcatgaag
     5401 agcaacatgt actttctaaa ccttcagaac atgataaaga gactagaatt gattctgagt
     5461 cttcaagaac tgattcagac agctcgatgc agacagagaa aataaaaaaa gacagttcag
     5521 atggaaataa aagtagtaat ctgaaatctg aagtaatatc agacaaatca aatacagtac
     5581 caaaattgtc ggaatctgat gatgaagtaa ataatcagaa gccattaact ttaccggaag
     5641 aacagaaatt gaaaagacag caaagtcaaa atgagcaaac aaaaacctat acatatggtg
     5701 atagcgaaca aaatgacaag tctaatcatg aaaatgattt aagtcatcat ataccatcga
     5761 taagtgatga taaagataac gtcatgagag aaaatcatat tgttgacgat aatcctgata
     5821 atgatatcaa tacaccatca ttatcaaaaa cagatgacga tcgaaaactt gatgaaaaaa
     5881 ttcatgttga agataaacat aaacaaaatg cagactcgtc tgaaacggtg ggatatcaaa
     5941 gtcagtcaac tgcatctcat cgtagcactg aaaaaagaaa tatttctatt aatgaccatg
     6001 ataaattaaa cggtcaaaaa acaaatacaa agacatcggc aaataataat caaaaaaagg
     6061 ctacatcaaa attgaacaaa gggcgcgcta cgaataataa ttatagtgac attttgaaaa
     6121 agttttggat gatgtattgg cctaaattag ttattctaat gggtattatt attctaattg
     6181 ttattttgaa tgccattttt aataatgtga acaaaaatga tcgcatgaat gataataatg
     6241 atgcagatgc tcaaaaatat acgacaacga tgaaaaatgc caataacaca gttaaatcgg
     6301 tcgttacagt tgaaaatgaa acatcaaaag attcatcatt acctaaagat aaagcatctc
     6361 aagacgaagt gggatcaggt gttgtatata aaaaatctgg agatacgtta tatattgtta
     6421 cgaatgcaca cgttgtcggt gataaagaaa atcaaaaaat aactttctcg aataataaaa
     6481 gtgttgttgg gaaagtgctt ggtaaagata aatggtcaga tttagctgtt gttaaagcaa
     6541 cttcttcaga cagttcagtg aaagagatag ctattggaga ttcaaataat ttagtgttag
     6601 gagagccaat attagtcgta ggtaatccac ttggtgtaga ctttaaaggc actgtgacag
     6661 aaggtattat ttcaggtctg aacagaaatg ttcctattga tttcgataaa gataataaat
     6721 atgatatgtt gatgaaagct ttccaaattg atgcatcagt aaatccaggt aactcgggtg
     6781 gtgctgtcgt caatagagaa ggaaaattaa taggtgtagt tgcagctaaa attagtatgc
     6841 caaacgttga aaatatgtca tttgcaatac ctgttaatga agtacaaaag attgtaaaag
     6901 atttagaaac aaaaggtaaa attgactatc ccgatgtagg tgttaaaatg aagaatattg
     6961 ccagtctaaa tagttttgaa agacaagcag ttaaattgcc aggaaaagtt aagaacggtg
     7021 ttgttgtaga tcaagttgac aacaatggtt tagcagatca atctggtctg aaaaaaggtg
     7081 atgtaattac tgaattagat ggcaaacttt tagaagatga tttacgcttt aggcagatta
     7141 tatttagtca taaagatgac ttgaaatcaa ttacagcgaa gatttataga gatggtaaag
     7201 agaaagaaat taatattaaa ctaaaataat gttgaggtga acaacgtgtc aatttttagc
     7261 cagtttttaa aaagatcaag ccctcaacaa ggtattgtat tgtactatat cgtcgcaatt
     7321 gtcattgcat ttttattatt aaacttaccg tatgttcata aaccaggtgt agaagtaaat
     7381 ccaattgaca cattatttgt tgccgtatcc ggaattagtg ttactggatt gtctccgata
     7441 agtattgtcg atacctattc tacatttgga caattaatta tcctcgtgat attaaatatt
     7501 ggtggaattg gcgtcatggc aattggtacg atgttatggg tggtactagg taaacatatt
     7561 ggaattagag aacgtcagtt aattatgtta gataataaca aaaacacaat gagtggtacc
     7621 gtcaaattga ttattgatat tgtaaaatca atatttgtaa tcgaactcgt aggagccatg
     7681 ttattagcat tttactttta tcgagataat ccagatttaa aatatgcaat catgcaaggt
     7741 gtttttgttt ctatttctgc cactaccaat ggtggattag atattacagg taagtcatta
     7801 attccttatg cacatgatta ttttgtacaa gcgatagtta tatttttaat aattttagga
     7861 tcaatcggct tcccagtatt attagaagtt aaagcttata ttcaaaatag ggttactaat
     7921 tttagatttt cattatttac taaaattacg acatcaacat atttattcct atttattgtt
     7981 ggggtattag ccattctatt atttgaacat aaccatgcgt tcaaaggttt aagttggcat
     8041 caatcgttat tctattcgct gtttcaatca gcgactacaa gaagtgcggg tcttcaaaca
     8101 attgatgtga caacactaag tgaccccact aatattatca tgggtatttt aatgtttata
     8161 ggatcttcgc caagttcggt tggtggcggt attcgtacaa caactttcgc tattttaatt
     8221 ttgtttttaa ttaactttag taataatgcc gataaaacat ccattaaagt ttacaataga
     8281 gaagtacaca ttatggatat tcaacgttca tttgcagtat ttacaatggc gacaatttta
     8341 acatttttag gaatgctaat tatatcagct actgaaaatg gtaagcttac atttttacaa
     8401 gtattttttg aagtcatgtc tgcatttgga acttgtggac tatcgcttgg tgtcacaagt
     8461 gatattagtg atatttctaa ggtcgtacta atgatactca tgtttatagg acgtgttggc
     8521 ttaatatcat ttatcattat gatagcagga cgtcgagaac cagataaatt ccattatcca
     8581 aaagaacgta ttcaaatagg ataatataat agcaatctaa gtttagttaa tgtagatttt
     8641 aactggaact tagattgctt ttttagtttg tatttttaac ttattttata agacgattgg
     8701 tttcgaaaat ggtaaactag taacaatgag aggtgtaaca tgatggaaaa aaatgaaaac
     8761 attaatgtag agattttaac tacgtcagat atgcatagtc atttcttaaa tggtgattat
     8821 ggttcaaata tttatagagc tggtacttat gttaaccaag taagagcaca aaatcatcgc
     8881 gtcattttat tagatagtgg cggaagttta gctggctcgt tagcggccta ttattatgct
     8941 attgttgcac cttataaacg acatccaatg ataaagttaa tgaacagaat gcattatgat
     9001 gctagcggtg tgagtccaag tgaattcaag tttggtttat catttttaac tcgttcaatt
     9061 gctttggcac gttttccatg gttatcagca aatattgaat acaatgttac taaggagcct
     9121 tatttttcaa ctccatattg tattaaacat tttggtgact taaaaattgc tatcgtaggc
     9181 gtcacagcag atggtttaat ggaaaatgag tattctgaaa tggagcaaga tgtatctatt
     9241 gaaaagacat tagtggcatc aaaacgttgg attagatata tccatgaagt tgaagagcca
     9301 gattttttga ttgtaattta tcatggtgga ttgaataaaa ttagtaatag tacgaaaaat
     9361 aaaaaggcaa gttcgaatga agctgaaaaa ttaatggaag aactcggtgt tatagattta
     9421 atgattacag ctcatcagca tcaaacaata gtaggtcaag atcatgaaac gtattatgtt
     9481 caggctggtc aagatgccaa agagcttgta catctttcga ttaattttaa aaagagaaca
     9541 acaacttatg atgttgaaag cattgattct aaagtgattg acttaaatga gtatgaagag
     9601 gatcaagaat tattagattt aacattctat gatagaaaag cagtggctta ttggtcacag
     9661 gaaatcataa gtgataaagg tttgatgtta tcagtaaatg ggttacaaga tttagtctgt
     9721 caaacacatc cattttcgca attattacat gatgcaattc accttgcatt tgataatgat
     9781 ataacatgtg tccacgtgcc tatgaacgga gagaaggggt tgagtggaca gattcgaaat
     9841 gaagatttgt atcatgcata cccatatcca gataagccaa tggatatgac aattagtggt
     9901 caaaatatca aagatatatt ggagtatagt tattcacatt tagattttgt taacgagcaa
     9961 ttaagcttaa caattattga tgaaacgtta tgtacaatgt ggcaaggatt caattatgag
    10021 attgatatga atcaagaacc tgggcaacga gtaatgttag atcaaattga tttgactaag
    10081 agttatagag ttacaatgac tgactattgt tatcgtaact acaagaatta tttaaaaaat
    10141 gctattatac atgaatcata cgatgaaaca atgagtacat taattgcaga gaagttaaga
    10201 gatccgaatt atcgtatttc atgtagtgat aattttatag ttaaaaacag gtaaatattg
    10261 ctatgtaata aattaattat aagttcaaca agtataaaaa cctttcttag agaagattat
    10321 ctaggaaagg tttttagaat tttaaaattg atataaaaga acagtgaaat tctcattcat
    10381 tatatataag taaacaaaaa agctaaaatg gtatatacca ttttagcaag taacggaaac
    10441 ggagggattc gaaccctcgc gccgctttcg cgacctacac ccttagcagg ggcgcctctt
    10501 cagccaactt gagtacgttt ccaatggctc cacaggtagg actcgaacct acgaccgatc
    10561 ggttaacagc cgatagctct accactgagc tactgtggat taatttgtat gtgtttgaat
    10621 atcaagcaca aatattatta taacaagatt aagttaattt gcaatagata ccttaaaaaa
    10681 atatgcagaa catttacact atatttataa aacattccgc atattttaaa aataaacaat
    10741 ttatttatct tcaaataaag aatagcgtgt caatgcttca aacacattca atgtggcttt
    10801 attataatct attggttggt cgggattttt tgatatcatg cgagattgtt tatctaccat
    10861 gtaatagtaa aaaatggaat tgttatattg atgccataaa ctgtggtatg aaacatgaag
    10921 aatgattgat tcattattaa taaatgtcac tttacattta cgatttttta attctttaat
    10981 actttcgata taatgcatat ttaaccaaat attttcattt tgtctgtcag aatgtgttgg
    11041 gaaaaaataa gttggaaata atggtgttag taaaataggt ggtttactag aaatgccagt
    11101 aatgcggttt gtctctgctt tcttgccaag ataagtatta ccataaaatt tgcaagagcg
    11161 ttcaatgatt ttctggactt taaaaggatt ttgaatacgc gttttgtcaa accgaattat
    11221 ttcactaccg tttctttgat cgtcatcatc aaatgctggt cgaataacca tgtctccttt
    11281 gcgtatcaca taaatatttt gagaatacat aagcaaaacc tcgctttatt aagttttaaa
    11341 ccattaatat aaaaatacaa ttaatgatat aaagatattt tataataaaa ttctgagttg
    11401 tcaagttatt attttggtga aaatgtacaa taatttttaa ctaatagtca gaatattcat
    11461 tgatttcaaa attagaaaaa tgtatactta ttataaatta aagaaaggta gtggtttcta
    11521 tgaaatacaa tactaatgtt aaacatacaa ctttagaagc gtttgtcaca actgtcaatg
    11581 atttgggtat tgaattaatt atcaatgaag cacttcgaga ggtaagaaaa cgacagctca
    11641 tagaacttat agatgacgca ctcgtcaata aagatgaagc agcatttaat caatatacgg
    11701 cagaatacaa aaatttggag gcatttctcg gtgaataaca ttgattcgaa ttccataacg
    11761 caatcataaa aggactattc ttaacatctc atacatgtaa gaatagtcct ttaatcgttt
    11821 atgacattaa tctaattaat tcatgtctat ctatgtcacc gaaataatga tataaatcat
    11881 attctgatag tgcttcttct atgtgctcaa agtcgtgtag acagccaact aatgcatttt
    11941 caagatcagt gacatctccg acaccaaaga aatcaccgaa tatttttgca tgttcgattt
    12001 tacctcgttt aacatcaaac ttaatttgta caaatccttt ttcaaatttt tcctcgcgtt
    12061 caaagttata tttagggttt ctaccataat tccattccca agttctatat ttgtcgttac
    12121 ttaacttttc aatattttcc caatcttcat ccgttaattt atattcttct acttcagttt
    12181 ctccaaagat agttttcaag atgatttttt taaattcttc aatttctaaa gggtcattta
    12241 aaaattcttg aatgtttgct acccgtttac gaacagattt aatacctttt gatttaatct
    12301 tagctggatt aacttttagt gcattctgaa cttcgtctaa atcactattt aacatcaacg
    12361 taccatgact aaacattcta tttttaactt taaccatagc attccctgag attttcgctt
    12421 gtccaacttg aatatcgtta cgaccagtta attcagcatt tacgcctaaa gattgtaatg
    12481 cttgaacaat tggttcagtg aatttttgga aattgtggaa actgttacca tcatcatctg
    12541 ttataaaact aaagtttaaa ttgccagtat catgataaac agcgccacca ccagaaattc
    12601 ttcttactac atcgatgttg tgagcatcga tatatgtctg atttacttcc tctatcgtat
    12661 tttgattctt tccaacaatg atagatggtc tatttatgta aaataaaaag taactttctt
    12721 ctgctggtaa attttttaaa acatattctt ccattgctaa gtttaaagtt ggatctgtaa
    12781 tattattatt actaatgaat ttcattacaa tctctccctt atatctatat atattctcta
    12841 cttatttatg ccttaacttt gctcaaaatg ccaattatat actcacaaat cattctaaat
    12901 atcggcttaa agttacattt tattattttt gattgttaat agtttatttg ttaagtgaat
    12961 tttagtacaa tataatttag ttatgattta aggaggactt aaaatgactg ttgcagaagt
    13021 gggtaacatt gttgagttta tggatggatt aagaggtcgt gttgaaaaaa tcaacgataa
    13081 ctctgttatt gttgacttaa caattatgga aaattttaat gaccttgatt taccggaaaa
    13141 aactgttatc aatcataaac gatataagat tgttgaataa gaaggtaagt tataatgaat
    13201 aaaatctcga aggctttaac ttggtttatt ataagtttca ttatatttca tctcatatta
    13261 tttattatgt ggggcgaaca ccaagaatac tggtatttat atacaggtat aatgctaatt
    13321 gctggtatca gttatgtatt ttatcaaaga gatattgaat ctaagcggtt gcttacatca
    13381 attggtgttg gtattattac ggcaattatt ttaattatgc ttcaactttt atcctcactt
    13441 ataacttcta atttaagtta tagttcatta attaaagaat tagcaagaac aggtgtcaat
    13501 tggaagtggc aaatgttagt aactttactt tttgtcattc catgtcacga gttatatatg
    13561 agaactgttt tacaaaaaga attaacacat ttttcaatac cgaaatgggt agctatatta
    13621 ataactgcaa tttgttctag ttcattattt atttatttag ataattggtg gatagtaaca
    13681 tttatatttg ttgcacaagt gatattatca ttaagttatg agtatactcg acgtatagca
    13741 acaacatccg ttgcacaaat tgtagctatt atattgttat taatttttaa tgcttaaata
    13801 agcttttctt agacgtatgt gttctaagaa aagctttttt gcttaagaat agcaatacga
    13861 agattcgagg catgcttcat tatagtggta atatttttta acagcaagcg gttattattg
    13921 agattaggtt ttagtcagtt tttattacta aatattttat aaaaatggta taatattttt
    13981 gagcaagttg aattgatggt ggctatctga gtaaaggggg gtggtgccta tggcattact
    14041 taacactttt gaaaggaaaa gcctattgtg atatctattg caaacgcatt acatttaatg
    14101 ttaagtttcg gtatgtttat cgtcactttc attggtatag tagtagcaat aataaattta
    14161 agcaataaaa aataaccatc atttcaaaac tttgacatag aatgatggtt aaagaaaatc
    14221 tca
//
LOCUS       DAMQYT010000053        14100 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMQYT010000053 DAMQYT010000000
VERSION     DAMQYT010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14100
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.053"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            43..1329
                     /gene="rseP"
                     /locus_tag="PGA33_001195"
     CDS             43..1329
                     /gene="rseP"
                     /locus_tag="PGA33_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDG6473017.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTEKKLTK
                     VSSETKYVLGFQPASEHTLFKPIVFGFKSFLIGSTYIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            1349..3052
                     /locus_tag="PGA33_001196"
     CDS             1349..3052
                     /locus_tag="PGA33_001196"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDG6473018.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQSLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            3316..7626
                     /locus_tag="PGA33_001197"
     CDS             3316..7626
                     /locus_tag="PGA33_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDG6473019.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            7916..8383
                     /gene="rimP"
                     /locus_tag="PGA33_001198"
     CDS             7916..8383
                     /gene="rimP"
                     /locus_tag="PGA33_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDG6473020.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            8404..9579
                     /gene="nusA"
                     /locus_tag="PGA33_001199"
     CDS             8404..9579
                     /gene="nusA"
                     /locus_tag="PGA33_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDG6473021.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDADTTESTEEVNDVSVETNVEKESE"
     gene            9600..9884
                     /locus_tag="PGA33_001200"
     CDS             9600..9884
                     /locus_tag="PGA33_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDG6473022.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            9881..10198
                     /locus_tag="PGA33_001201"
     CDS             9881..10198
                     /locus_tag="PGA33_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HDG6473023.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            10203..12320
                     /gene="infB"
                     /locus_tag="PGA33_001202"
     CDS             10203..12320
                     /gene="infB"
                     /locus_tag="PGA33_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDG6473024.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIIQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            12706..13056
                     /gene="rbfA"
                     /locus_tag="PGA33_001203"
     CDS             12706..13056
                     /gene="rbfA"
                     /locus_tag="PGA33_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDG6473025.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            13225..>14100
                     /gene="truB"
                     /locus_tag="PGA33_001204"
     CDS             13225..>14100
                     /gene="truB"
                     /locus_tag="PGA33_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDG6473026.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHP"
BASE COUNT         5151 a         1909 c         2816 g         4224 t
ORIGIN      
        1 ttttaaaata aaagaaattt ataaacaatc gaggtgtagc gagtgagcta tttagttaca
       61 ataattgcat ttattattgt ttttggtgta ctagtaactg ttcatgaata tggccatatg
      121 ttttttgcga aaagagcagg cattatgtgt ccagaatttg cgatcggtat ggggccaaaa
      181 atttttagtt ttagaaaaaa tgaaacactt tacactatta ggttattgcc tgttggtgga
      241 tatgttcgta tggcaggaga tggcttagaa gagccaccag tcgagcccgg tatgaacgtt
      301 aaaattaaac ttaatgaaga aaatgaaata acacatatca tattagatga tcatcataag
      361 tttcaacaaa ttgaagcgat cgaagttaaa aaatgtgatt ttaaggatga cttattcata
      421 gaaggtatca ctgcttatga taatgaaaga catcatttta aaattgctag aaagtctttc
      481 tttgttgaaa atggtagctt agttcaaatt gctccgagag acagacaatt tgcacataaa
      541 aagccatggc cgaaattttt aacattattt gcgggaccgt tatttaactt tatattagct
      601 ttagtcctat ttattggtct tgcatattat caaggcacgc ctacgtctac tgtagaacaa
      661 gtcgcagata agtatccagc tcaacaagca ggattacaaa aaggtgataa gatcgtccaa
      721 attggcaaat ataaaatatc tgaatttgat gatgttgata aggcgttaga taaagttaaa
      781 gataataaga cgactgttaa atttgaacgt gatggtaaaa caaagtcagt tgaattaaca
      841 cctaaaaaga ctgaaaaaaa actgactaaa gtaagttcag agacgaagta tgttctcgga
      901 ttccaaccag cgagtgaaca tacacttttt aaaccaattg tattcggatt taaaagcttt
      961 ttaatcggta gtacttatat ttttacagct gtagtaggta tgttggctag tatatttacg
     1021 ggcggattct catttgatat gttaaatggt ccggttggta tttatcataa cgtcgactca
     1081 gttgttaaag cgggtatcat tagcttaatt ggttacactg cgttattaag tgtaaactta
     1141 ggtattatga atttaattcc tattcctgca ctagacggtg gtcgtatttt atttgttata
     1201 tatgaagcga ttttcagaaa accagttaat aaaaaagcgg aaacaacgat tattgctatt
     1261 ggtgccattt tcatggtcgt tataatgata ttagtaacgt ggaatgatat tcgacgatat
     1321 ttcttataat ttaggaggat aaataattat gaagcaatcc aaagttttta taccaacgat
     1381 gcgtgacgtg ccatcagaag cagaagcaca aagtcatcgt ttattattga aatcgggttt
     1441 gataaaacaa agtacaagtg ggatttatag ttatttaccg ctagcaacac gtgtgttaaa
     1501 taatattact gcaattgtgc gacaagaaat ggaacgtatc gattctgttg aaattttaat
     1561 gccagcgtta caacaagctg aattatggga agaatcagga cgttggggtg catatggccc
     1621 agaattaatg cgtttacaag atagacatgg aagacaattt gcattaggtc caacacatga
     1681 agaattagtt acatcaatag taagaaatga attgaaatca tacaaacaat taccgatgac
     1741 attattccaa attcaatcta aattccgtga tgaaaagaga ccacgttttg gtttacttcg
     1801 tgggcgtgaa tttattatga aagatgcata ttcattccat gctgacgagg catcattaga
     1861 tcaaacgtat caagatatgt atcaagcgta tagccgtatt tttgagagag ttggcattaa
     1921 cgcaagacca gtagttgcag attcaggtgc tataggcggt agccatacac atgaatttat
     1981 ggcattaagt gctatcggtg aggatacaat cgtttacagt aaagaaagtg attatgctgc
     2041 taacatcgaa aaagcagaag tcgtttacga accaaatcat aagcatacta ctgtgcaacc
     2101 tttagaaaaa attgaaacac caaatgttaa gactgcgcaa gaattggcag acttcttagg
     2161 tagaccagta gatgaaatcg ttaaaacgat gattttcaaa gttgatggcg aatatattat
     2221 ggttttagtg cgtggccatc atgaaattaa tgacattaaa ttaaaatctt atttcggcac
     2281 agataatatt gaattagcaa cacaagacga aattgttaat ttagttggtg caaatcctgg
     2341 ttcactaggt cctgtaattg ataaagaaat caaaatttat gcagataatt ttgtgcaaga
     2401 tttaaataat ttagttgtcg gtgctaacga agatggttat cacttaatta atgtaaatgt
     2461 aggtagagac ttcaacgttg atgaatatgg cgatttccgt tttattttag aaggcgaaaa
     2521 gttaagtgat ggttcaggcg ttgcacattt tgctgaaggt attgaagttg gtcaagtatt
     2581 caaattgggt actaagtatt cagaatcaat gaatgctaca ttcttagata accaaggaaa
     2641 agctcaatct ttaattatgg gttgttacgg aattggaatt tctagaacgc taagtgcgat
     2701 tgttgaacaa aatcacgatg ataatggaat tgtttggcct aaatcagtta ctccgtttga
     2761 tttacattta atttctatta atcctaagaa agatgatcaa cgagaactag cagatgcact
     2821 atatgctgaa tttaatacta aatttgatgt gttgtacgat gatcgtcagg aacgtgcagg
     2881 tgttaaattt aatgatgccg atttaattgg tttaccactg cgaattgttg ttggtaaacg
     2941 tgcatcggaa ggtattgtag aagttaaaga acgtttaaca ggtgatagcg aagaagttca
     3001 cattgatgac ttaatgactg tcattacaaa taaatatgat aacttaaaat aattaagatc
     3061 gaatgaatta taagagtagg aaaaagctga aagaaatctg atgcttatgt cctgctctta
     3121 ttatttttga tataatgatt attcgatgaa aaatgactga agacatagta taattaaaga
     3181 taaatttgtt ttaacaatat aatgattagc caaatataaa gcatttaatt ttctatcatt
     3241 actatgctca cataatctaa atattgttcg aacacgtaaa agtaatttct atttaaggtg
     3301 gtaattgtct tggcaatgac agagcaacaa aaatttaaag tgcttgctga tcaaattaaa
     3361 atttcaaatc aattagatgc tgaaatttta aattcaggtg aactgacacg tatagatgtt
     3421 tctaacaaaa acagaacatg ggaatttcat attacattac cacaattctt agctcatgaa
     3481 gattatttat tatttataaa tgcaatagag caagagttta aagatatcgc caacgttaca
     3541 tgtcgtttta cggtaacaaa tggcacgaat caagatgaac atgcaattaa atactttggg
     3601 cactgtattg accaaacagc tttatctcca aaagttaaag gtcaattgaa acagaaaaag
     3661 cttattatgt ctggaaaagt attaaaagta atggtatcaa atgacattga acgtaatcat
     3721 tttgataagg catgtaatgg aagtcttatc aaagcgttta gaaattgtgg ttttgatatc
     3781 gataaaatca tattcgaaac aaatgataat gatcaagaac aaaacttagc ttctttagaa
     3841 gcacatattc aagaagaaga cgaacaaagt gcacgattgg caacagagaa acttgaaaaa
     3901 atgaaagctg aaaaagcgaa acaacaagat aacaacgaaa gtgctgtcga taagtgtcaa
     3961 attggtaagc cgattcaaat tgaaaatatt aaaccaattg aatctattat tgaggaagag
     4021 tttaaagttg caatagaggg tgtcattttt gatataaact taaaagaact taaaagtggt
     4081 cgccatatcg tagaaattaa agtgactgac tatacggact ctttagtttt aaaaatgttt
     4141 actcgtaaaa acaaagatga tttagaacat tttaaagcgc taagtgttgg taaatgggtt
     4201 agggctcaag gtcgtattga agaagataca tttattagag atttagttat gatgatgtct
     4261 gatattgaag agattaaaaa agcgacaaaa aaagataagg ctgaagaaaa gcgtgtagaa
     4321 ttccacttgc atactgcaat gagccaaatg gatggtatac ccaatattgg tgcgtatgtt
     4381 aaacaggcag cagactgggg acatccagcc attgcggtta cagaccataa tgttgtgcaa
     4441 gcatttccag atgctcacgc agcagcggaa aaacatggca ttaaaatgat atacggtatg
     4501 gaaggtatgt tagttgatga tggtgttccg attgcataca aaccacaaga tgtcgtatta
     4561 aaagatgcta cttatgttgt gttcgacgtt gagacaactg gtttatcaaa tcagtatgat
     4621 aaaatcatcg agcttgcagc tgtgaaagtt cataacggtg aaatcatcga taagtttgaa
     4681 aggtttagta atccgcatga acgattatcg gaaacgatta tcaatttgac gcatattact
     4741 gatgatatgt tagtagatgc ccctgagatt gaagaagtac ttacagagtt taaagaatgg
     4801 gttggcgatg cgatattcgt agcgcataat gcttcgtttg atatgggctt catcgatacg
     4861 ggatatgaac gtcttgggtt tggaccatca acgaatggtg ttatcgatac tttagaatta
     4921 tctcgtacga ttaatactga atatggtaaa catggtttga atttcttggc taaaaaatat
     4981 ggcgtagaat taacgcaaca tcaccgtgcc atttatgata cagaagcaac agcttacatt
     5041 ttcataaaaa tggttcaaca aatgaaagaa ttaggcgtat taaatcataa cgaaatcaac
     5101 aaaaaactca gtaatgaaga tgcatataaa cgtgcaagac ctagtcatgt cacattaatt
     5161 gtacaaaacc aacaaggtct taaaaatcta tttaaaattg taagtgcatc attggtgaag
     5221 tatttctacc gtacacctcg aattccacgt tcattgttag atgaatatcg tgagggatta
     5281 ttggtaggta cagcgtgtga tgaaggtgaa ttatttacgg cagttatgca gaaggaccag
     5341 agtcaagttg aaaaaattgc caaatattat gattttattg aaattcaacc accggcactt
     5401 tatcaagatt taattgatag agagcttatt agagatactg aaacattaca tgaaatttat
     5461 caacgtttaa tacatgcagg tgacacagcg ggtatacctg ttattgcgac aggaaatgca
     5521 cactatttgt ttgaacatga tggtatcgca cgtaaaattt taatagcatc acaacccggc
     5581 aatccactta atcgctcaac tttaccggaa gcacatttta gaactacaga tgaaatgtta
     5641 aacgagtttc attttttagg tgaagaaaaa gcgcatgaaa ttgttgtgaa aaatacaaac
     5701 gaattagcag atcgaattga acgtgttgtt cctattaaag atgaattata cacaccgcgt
     5761 atggaaggtg ctaacgaaga aattagagaa ctaagttatg caaatgcgcg taaactgtat
     5821 ggtgaagacc tgcctcaaat cgtaattgat cgattagaaa aagaattaaa aagtattatc
     5881 ggtaatggat ttgcggtaat ttacttaatt tcgcaacgtt tagttaaaaa atcattagat
     5941 gatggatact tagttggttc ccgtggttca gtaggttcta gttttgtagc gacaatgact
     6001 gagattactg aagtaaaccc gttaccgcca cactatattt gtccgaactg taaaacgagt
     6061 gaatttttca atgatggttc agtaggatca ggatttgatt tacctgataa gacgtgtgaa
     6121 acttgtggag cgccacttat taaagaagga caagatattc cgtttgaaac atttttagga
     6181 tttaagggag ataaagttcc tgatatcgac ttaaacttta gtggtgaata tcaaccgaat
     6241 gcccataact acacaaaagt attatttggt gaggataaag tattccgtgc aggtacaatt
     6301 ggtactgttg ctgaaaagac tgcttttggt tatgttaaag gttatttgaa tgatcaaggt
     6361 atccacaaaa gaggtgctga aatagatcga ctcgttaaag gatgtacagg tgttaaacgt
     6421 acaactggac agcatccagg gggtattatt gtagtacctg attacatgga tatttatgat
     6481 tttacgccga tacaatatcc tgccgatgat caaaattcag catggatgac gacacatttt
     6541 gatttccatt ctattcatga taatgtatta aaacttgata tacttggaca cgatgatcca
     6601 acaatgattc gtatgcttca agatttatca ggaattgatc caaaaacaat acctgtagat
     6661 gataaagaag ttatgcagat atttagtaca cctgaaagtt tgggtgttac tgaagatgaa
     6721 attttatgta aaacaggtac atttggggta ccagaattcg gtacaggatt cgtgcgtcaa
     6781 atgttagaag atacaaagcc aacaacattt tctgaattag ttcaaatctc aggattatct
     6841 catggtacag atgtgtggtt aggcaatgct caagaattaa ttaaaaccgg tatatgtgat
     6901 ttatcaagtg taattggttg tcgtgatgat atcatggttt atttaatgta tgctggttta
     6961 gaaccatcaa tggcttttaa aataatggag tcagtacgta aaggtaaagg tttaactgaa
     7021 gaaatgattg aaacgatgaa agaaaatgaa gtgccagatt ggtatttaga ttcatgtctt
     7081 aaaattaagt acatgttccc taaagcccat gcagcagcat acgttttaat ggcagtacgt
     7141 atcgcatatt tcaaagtaca tcatccactt tattactatg catcttactt tacaattcgt
     7201 gcgtcagact ttgatttaat cacgatgatt aaagataaaa caagcattcg aaatactgta
     7261 aaagacatgt attctcgcta tatggatcta ggtaaaaaag aaaaagacgt attaacagtc
     7321 ttggaaatta tgaatgaaat ggcgcatcga ggttatcgaa tgcaaccgat tagtttagaa
     7381 aagagtcagg cgttcgaatt tatcattgaa ggcgatacac ttattccgcc gttcatatca
     7441 gtgcctgggc ttggcgaaaa cgttgcgaaa cgaattgttg aagctcgtga cgatggccca
     7501 tttttatcaa aagaagattt aaacaaaaaa gctggattat ctcagaaaat tattgagtat
     7561 ttagatgagt taggctcatt accgaattta ccagataaag ctcaactttc gatatttgat
     7621 atgtaaaatg aaataatcaa ggtatttatt taatgcgtat ggcgtagtca aagaaataca
     7681 aaattgttgc tggacacaaa attatgcccg tatttctttt caatgtctta cgagtctatt
     7741 caaatgtaat ggtgaaataa aggaacaaac ttttacaaga atctctgatt aatagtgaag
     7801 tcatttgttt caagcataaa cttatgctat aattaagttg cttaaaaatt agtgaactca
     7861 ggcagaagag tgggagattc ccgctctttt ctatttgcca aaaagggagg cctgtatgag
     7921 taaaattaca gaacaagtag aagtgattgt taaaccaatt atggaagact tgaattttga
     7981 acttgtagac gttgaatatg tcaaagaggg tagagatcat tttcttagaa tctctattga
     8041 taaagaaggt ggcgtagatt taaatgattg tacgctagct tctgaaaaaa taagtgaagc
     8101 tatggatgca aatgatccta ttcctgaaat gtattattta gacgtagcgt cacctggtgc
     8161 agaacgtcca attaaaaaag aacaagattt ccaaaatgca ataactaaac ctgtatttgt
     8221 ttctttatat gtaccaattg aaggtgaaaa ggaatggtta ggcattttac aagaagtcaa
     8281 taatgaaaca attgtagtac aagttaaaat caaagcaaga acgaaagata tagagatacc
     8341 gagagacaaa atagcaaaag cacgtcacgc agttatgatt taacgtgatg aggaggaaaa
     8401 aacgtgtcaa gtaatgaatt attattagct actgagtatt tagaaaaaga aaagaagatt
     8461 cctagagcag tattaattga tgctattgaa gcagctttaa ttactgcata caaaaagaat
     8521 tatgatagtg caagaaatgt ccgtgtggaa ttaaatatgg atcaaggtac tttcaaagtt
     8581 atcgctcgta aagatgttgt tgaagaagta tttgacgaca gagatgaagt ggatttaagt
     8641 acagcgcttg ttaaaaaccc tgcatatgaa attggtgata tatacgaaga agatgtaaca
     8701 cctaaagatt ttggtcgtgt aggtgctcaa gcagcgaaac aagcagtaat gcaacgtctt
     8761 cgtgatgctg aacgtgaaat tttatttgaa gaatttatag acaaagaaga agacatactt
     8821 actggaatta ttgaccgtgt tgaccatcgt tatgtatatg tgaatttagg tcgtatcgaa
     8881 gctgttttat ctgaagcaga aagaagtcct aacgaaaaat atattcctaa cgaacgtatc
     8941 aaagtatatg ttaacaaagt ggaacaaacg acaaaaggtc ctcaaatcta tgtttctcgt
     9001 agccatccag gtttattaaa acgtttattt gaacaagaag ttccagaaat ttacgatggt
     9061 actgtaattg ttaaatcagt agcacgtgaa gctggcgatc gctctaaaat tagtgtcttc
     9121 tctgaaaaca atgatataga tgctgttggt gcatgtgttg gtgctaaagg cgcacgtgtt
     9181 gaagctgttg ttgaagagct aggtggtgaa aaaatcgaca tcgttcaatg gaatgaagat
     9241 ccaaaagtat ttgtaaaaaa tgctttaagc ccttctcaag ttttagaagt tattgttgat
     9301 gaaacaaatc aatctacagt agttgttgtt cctgattatc aattgtcatt agcgattggt
     9361 aaaagaggac aaaacgcacg tctagctgct aaattaaccg gctggaaaat tgatattaaa
     9421 tcagaaacag atgcgcgtga agcgggtatc tatccagtag ttgaagctga aaaagtaact
     9481 gaagaagatg ttgctttaga agatgctgac acaacagaat caaccgaaga ggtaaatgat
     9541 gtttcagttg aaacaaatgt agagaaagaa tctgaataat aggttggagt gaagtatcta
     9601 tgaaaaagaa aaaaattccg atgcgaaaat gtattctttc aaatgaaatg catcccaaaa
     9661 aagatatgat tcgtgttgtt gttaataaag aaggcgaaat ctttgcggat gttactggaa
     9721 agaaacaagg ccgtggcgca tatgtttcta aagatgttgc tatggttgaa aaagcacaac
     9781 aaaaagaaat tttagaaaaa tattttaaag catctaaaga gcaattggat cctgtttaca
     9841 aagaaattat tagattaatt tatagagaag agatcccaaa atgagtatag atcaaatatt
     9901 aaacttttta ggattagcaa tgagagctgg taaagtaaaa acaggtgaat cagtcattgt
     9961 taatgagatt aaaaaaggaa atttgaagct cgttattgtt gcaaatgatg cgtctgataa
    10021 tacagctaaa ttaattacag ataaatgtaa gagttacaaa gttccattca gaaagtttgg
    10081 aaatcgaaat gaattgggaa tagcacttgg aaaaggtgag cgtgttaatg tagggattac
    10141 tgacccaggc tttgctaaaa agttgctatc aatgatagat gaatatcata aggagtgatt
    10201 atatgagtaa acaaagaatt tacgaatatg cgaaagaatt aaatctaaag agtaaagaga
    10261 ttatagatga gttaaaaagc atgaatattg aggtttcaaa tcatatgcaa gctttggaag
    10321 atgaccaaat taaagcatta gataaaaagt tcaaaaaaga acaaaagaac gacaataaac
    10381 aaagcactca aaataatcac caaaaatcaa acaatcaaaa ccaaaataaa gggcaacaaa
    10441 aagataacaa aaagaatcaa caacaaaata ataaaggcaa caaaggcaat aaaaagaata
    10501 atagaaataa taagaaaaat aacaagaata ataaaccaca aaatcaacca gctgctccaa
    10561 aagaaatacc atcaaaagtg acatatcaag aaggtattac agtaggcgaa tttgcggata
    10621 aattaaatgt tgaatcatca gaaattatca aaaaattatt cttacttggt attgttgcta
    10681 atatcaatca atcattaaat caagaaacaa tcgaattaat tgccgatgat tatggcgttg
    10741 aggttgaaga agaagttgtg attaatgaag aagacttatc aatctatttc gaagacgaaa
    10801 aagatgatcc agaggcaatt gagagaccag cagttgtaac aattatggga catgttgacc
    10861 atggtaaaac gactttatta gattcaattc gtcatacaaa agttacagca ggtgaagcag
    10921 gcggaatcac tcaacatatt ggtgcatatc aaattgaaaa cgatggcaaa aaaatcactt
    10981 tcttagatac accgggacat gctgcattta caacgatgcg tgcgcgtggt gcacaagtaa
    11041 cagatattac tattttagta gtagcagctg acgatggtgt tatgccacaa acaattgaag
    11101 caattaacca tgctaaagaa gcagaagtac caattattgt tgcagtaaat aaaattgata
    11161 aaccaacttc aaatcctgat cgagttatgc aagaattaac tgaatatggt ttaattcctg
    11221 aagattgggg cggcgaaaca attttcgttc cactttctgc attaagtggt gatggtatcg
    11281 acgatttatt agaaatgata ggattagttg cagaagttca agaacttaaa gcaaatccta
    11341 aaaaccgtgc tgttggtaca gttatcgaag ctgaattaga taaatcacgt ggtccttctg
    11401 catcattatt agtacaaaac ggtacattaa atgttggtga tgcgattgta gttggtaata
    11461 cttacggccg tatccgtgca atggttaatg acttaggtca aagaatcaaa acggctggtc
    11521 catcaacgcc tgttgaaatt acaggtatta atgatgtgcc acaagctggg gatcgctttg
    11581 ttgtatttag tgatgaaaaa caagctcgtc gtattggtga atcaagacac gaagctagca
    11641 ttatacaaca acgtcaagaa agtaaaaatg tttcattaga taacctgttt gaacaaatga
    11701 aacaaggtga aatgaaagat ttaaacgtta ttattaaagg tgatgttcaa ggttctgttg
    11761 aagctttagc tgcatcatta atgaaaattg atgttgaagg cgtaaatgtt cgtatcattc
    11821 atacagcggt tggtgcaatt aatgagtcag acgtgacact tgctaatgcc tcaaatggta
    11881 ttatcattgg tttcaatgtt cgtccagaca gtggtgcaaa acgtgctgca gaagctgaaa
    11941 atgttgatat gcgtttacac agagttattt ataatgttat cgaagaaatt gaatcagcga
    12001 tgaaaggttt acttgatcca gaatttgaag aacaagttat cggacaagct gaagttcgtc
    12061 aaacattcaa agtttctaaa gttggtacta ttgctggatg ttatgttact gaaggtaaaa
    12121 ttacgcgaaa tgctggtgta cgtattattc gtgatggtat tgttcaatat gaaggcgaat
    12181 tagatacact taaacgtttc aaagatgatg ctaaggaagt tgcaaaaggt tatgaatgtg
    12241 gtattacaat tgaaaactac aatgacctta aagaaggcga tgttatcgaa gcatttgaaa
    12301 tggttgaaat taagcgttaa ttaaataaat tacaagctaa aagtatagtt aagattgata
    12361 tgctccctat aaatattgca ctttttaagt gtctacttta tagggagcat atttgatact
    12421 agcttttggt tttttattag aatagattac ctattaaaag ttacgttata tggacatgat
    12481 tttgtataaa attttgtggt ggcctagaat gatttttaat gacaaaatat aatgtcgact
    12541 attattggaa aattttctgt tgaaatgcct atcttacggc aaactttatt tgattttata
    12601 ggcttaattt attaaaataa cgtgtgagct aaaataattg tttaagcatt gttacactaa
    12661 aaaatgcaaa taacaattga acttaaagat aaagaggtga caagaatgag cagtatgaga
    12721 gcagagcgtg ttggtgaaca aatgaagaag gaattaatgg atatcatcaa caataaagtc
    12781 aaagatcctc gagttggttt tattacaatt acagatgttg ttttaacaaa tgatttatcg
    12841 caggctaaag tatttttaac tgtattaggt aacgataaag aagtagaaaa tacatttaaa
    12901 gcacttgata aagcaaaagg cttcattaag tctgaattag gttctagaat gcgattacgt
    12961 attatgccgg aattaatgta tgaatatgat caatcaatcg aatatggtaa taaaattgaa
    13021 cgaatgattc aagatttaca caaacaagat agataattta gtgttaggta tctggaaaat
    13081 gtttgataat ttcttaatat cggtatatta acattaaaca gttaatacat agatgtgtag
    13141 aaatagttaa cattttccag tttttttatg aataaattta gttgatacgc tattaaaata
    13201 tattttaaaa aagaaggtga ctatatgtat aatgggatat taccagtata taaagagcgc
    13261 ggtttaacaa gtcatgacgt tgtattcaaa ttgcgtaaaa tattaaaaac taaaaaaata
    13321 ggtcacacgg gtacgcttga tcccgaagtt gcaggcgtgt taccggtatg tataggtaat
    13381 gcaacgagag ttagtgatta tgttatggat atgggcaaag cttatgaagc aactgtatcg
    13441 ataggaagaa gtacaacgac tgaagatcaa acgggtgata cattggaaac aaaaggtgta
    13501 cactcagcag attttaataa ggacgatatt gaccgattgt tagaaagttt taaaggtatc
    13561 attgaacaaa ttccgccgat gtactcatcc gtcaaagtaa atggtaaaaa attatatgaa
    13621 tatgcgcgta ataatgaaac agttgaaaga ccaaagcgta aagttaatat taaagacatt
    13681 gggcgtatat ctgaattaga ttttaaagaa aatgagtgtc attttaaaat acgcgtcatc
    13741 tgtggtaaag gtacatatat tagaacgcta gcaactgata ttggtgtgaa attaggcttt
    13801 ccggcacata tgtcgaaatt aacacgaatc gagtctggtg gatttgtgtt gaaagatagc
    13861 cttacattag aacaaataaa agaacttcat gagcaggatt cattgcaaaa taaattgttt
    13921 cctttagaat atggattaaa gggtttgcca agcattaaaa ttaaagattc gcacataaaa
    13981 aaacgtattt taaatgggca gaaatttaat aaaaatgaat ttgataacaa aattaaagac
    14041 caaattgtat ttattgatga tgattcagaa aaagtattag caatttatat ggtacaccct
//
LOCUS       DAMQYT010000054        13783 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMQYT010000054 DAMQYT010000000
VERSION     DAMQYT010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13783
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.054"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            330..2939
                     /gene="adhE"
                     /locus_tag="PGA33_001205"
                     /gene_synonym="adhC"
     CDS             330..2939
                     /gene="adhE"
                     /locus_tag="PGA33_001205"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDG6473027.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQKTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
     gene            3284..3952
                     /gene="capA"
                     /locus_tag="PGA33_001206"
     CDS             3284..3952
                     /gene="capA"
                     /locus_tag="PGA33_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HDG6473028.1"
                     /translation="MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKY
                     QANTQILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSK
                     LSSMLTITNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKA
                     DGTAVKVAPKTVVNLIGAFFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSI
                     QKFN"
     gene            3968..4654
                     /gene="cap8B"
                     /locus_tag="PGA33_001207"
     CDS             3968..4654
                     /gene="cap8B"
                     /locus_tag="PGA33_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HDG6473029.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIGSERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            4657..5421
                     /gene="cap8C"
                     /locus_tag="PGA33_001208"
     CDS             4657..5421
                     /gene="cap8C"
                     /locus_tag="PGA33_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HDG6473030.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMMDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVINGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            5441..7265
                     /gene="cap8D"
                     /locus_tag="PGA33_001209"
                     /pseudo
     CDS             5441..7265
                     /gene="cap8D"
                     /locus_tag="PGA33_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
     gene            7255..8283
                     /gene="cap8E"
                     /locus_tag="PGA33_001210"
     CDS             7255..8283
                     /gene="cap8E"
                     /locus_tag="PGA33_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HDG6473031.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKQFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVYYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            8296..9405
                     /gene="cap8F"
                     /locus_tag="PGA33_001211"
     CDS             8296..9405
                     /gene="cap8F"
                     /locus_tag="PGA33_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HDG6473032.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            9409..10533
                     /gene="cap8G"
                     /locus_tag="PGA33_001212"
     CDS             9409..10533
                     /gene="cap8G"
                     /locus_tag="PGA33_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HDG6473033.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGL
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            10536..11162
                     /locus_tag="PGA33_001213"
     CDS             10536..11162
                     /locus_tag="PGA33_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HDG6473034.1"
                     /translation="MRIAIEKIIGLLKNQSSKESNVKIHRLAYITNSKFDGNNYIDRW
                     CKIRNSHIGEYSYIGFGSDFNNVEVGRYCSISSDVKIGLGKHPTHFFSSSPIFYSNNN
                     PFNIKQKFIDFNDQPSRTTIKNDVWIGANVIIMDGLTINTGAVIAAGSVVTKNVGAYE
                     VVGGVPAKVIKKRFDNKTIEKLLESKWWEKTPDKLKGFSVEYLNKKDT"
     gene            11167..12276
                     /locus_tag="PGA33_001214"
     CDS             11167..12276
                     /locus_tag="PGA33_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDG6473035.1"
                     /translation="MRILNIVSSNIVQDPRVLKQIETIKGVTDDYKIVGMNNSQATNK
                     RLENLDCNYRLLGSKVDPKNILSKLIKRIRFATGVIREIKAYKPDVIHANDFDVLLMV
                     YLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKE
                     YYQSKGYKKEANVITNAPILNDSREFKEIENFKEIVYQGQIVMDRGYEEFIIASSAFK
                     QNAPSFIIRGFGPHEEVIKELISYNPENIRLDKPVEVKELVDKLAESNVGVVLTKPVS
                     INFEYTVSNKIFECIHAGLPVILSPVKEHIYLNEKYKFGIVLKEVTPLEIEKAVRKLR
                     DNHDLFNHLRQNAIKASKILNWQIESERLVELYKF"
     gene            12290..13456
                     /locus_tag="PGA33_001215"
     CDS             12290..13456
                     /locus_tag="PGA33_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDG6473036.1"
                     /translation="MKFFVLCAIISMNIFIVISTFTKEVLGFPIEPVYYSTMVGIALI
                     TTVFAIYKIIVTQEIPRGLILLIAICLLYLAFYYFSPDKEEKLAKNNILFFLTWAVPA
                     AISGIYIKYINKATVERFFKLVFFIFSVSFIFVILIPKLTGEIPSYINFGLMNYQNAS
                     YLSAFTAGLGIYFIMKGSVKHKWIYVLFTIIDIPIVFIPGGRGGAILLILYGLFAFIL
                     ITFKRGIPIAVKSIMYIFALSISSVLIYFLFTKGSNTRTFSYLQGGTLNLEGTSGRGP
                     IYEKGIYFIQQSSLLGYGPFNYYKLIGNIPHNIIIELILSFGLLGFFIIMICILLLVY
                     KMIRNYDPNTIDLLVMFIAIYPITLLMFSSNYLVVSEFWFVLFYFITKGRRHHG"
     gene            13449..>13783
                     /locus_tag="PGA33_001216"
     CDS             13449..>13783
                     /locus_tag="PGA33_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDG6473037.1"
                     /translation="MAKKVFIMDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSI
                     LTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFASILLISILIESIALIIVFL
                     YFFNLNIID"
BASE COUNT         5029 a         1922 c         2591 g         4241 t
ORIGIN      
        1 atttgtaaca tctgttgata ggtatcgaca aggacctttt aatgttgtgt tttgtgcgat
       61 ataacaagct ttttaggcta ttaaagaata ttcagaatta aatatataat tatgaataaa
      121 ttatgtcatt gaaaaaatta tgattataaa atcattctaa aaacaccgaa ataacaatga
      181 tttcatgaaa acatttattt taaaatttga tatttgttca aataatattc gaaattaaac
      241 ttttttgtat agaattttct ttatatcctg agagacatgt actataatgt ttgtgaaata
      301 attcacaaag tataaaggag tggttgtata tgttaactat acctgaaaaa gaaaatcgtg
      361 gatcgaaaga acaagaagtg gcaattatga ttgatgctct agctgacaaa gggaaaaaag
      421 cattagaagc attatctaaa aagtcacaag aagaaattga tcatattgtt catcaaatga
      481 gcttagcagc tgttgatcaa catatggtgc tagcaaaatt agcacatgaa gaaactggaa
      541 gaggtatata cgaagataaa gcgattaaaa atttatacgc ttctgaatat atatggaatt
      601 caataaaaga caataagaca gtagggatta ttggtgaaga taaagaaaaa ggattaacgt
      661 atgtagcgga accaattggt gttatttgtg gtgttacgcc aacaacaaat cctacgtcga
      721 caactatttt taaagcgatg attgcaatta agacaggaaa tccaatcatt tttgcattcc
      781 atccaagtgc acaagaatcg tcgaagcgtg cagcagaagt tgtattagaa gcggcaatga
      841 aggcaggtgc acctaaagat attattcagt ggattgaagt gccttctatc gaagcaacaa
      901 aacaattaat gaatcacaaa ggtattgcat tagttctagc aacaggtggt tcgggcatgg
      961 ttaagtctgc atattcaact ggcaaaccgg cattaggtgt gggaccaggt aacgtgccgt
     1021 cttacattga aaaaacagca cacattaaac gtgcagtaaa tgatatcatt ggttcaaaaa
     1081 catttgataa tggtatgatt tgtgcttctg aacaagttgt agtcattgat aaagaaattt
     1141 ataaagatgt tactaatgaa tttaaagcac atcaagcata ctttgttaaa aaagatgaat
     1201 tacaacgctt agaaaatgca attatgaatg aacaaaaaac aggtattaag cctgatattg
     1261 tcggtaaatc tgcagttgaa atagctgaat tagcaggtat acctgtcccc gaaaatacaa
     1321 aacttatcat agccgaaatt agcggtgtag gttcagacta tccgttatct cgtgaaaaat
     1381 tatctccagt attagcctta gtaaaagccc aatctacaaa acaagcattt caaatttgtg
     1441 aagacacact acattttggt ggattaggac acacagccgt tatccataca gaagatgaaa
     1501 cattacaaaa agattttgga ctaagaatga aagcttgtcg tgtacttgta aatacaccat
     1561 cagcggttgg aggtattggt gatatgtata acgaattgat tccgtcttta acattaggtt
     1621 gtggttccta cggtagaaac tcaatttcac ataatgttag tgcgacagat ttattaaaca
     1681 ttaaaacgat tgctaaacga cgtaataata ctcaaatttt caaggtgcct gctcaaattt
     1741 attttgaaga aaatgcaatc atgagtctaa caacaatgga caagattgaa aaagtgatga
     1801 ttgtctgtga ccctggtatg gtagaattcg gttatacaaa aacagttgag aatgtattaa
     1861 gacaaaaaac ggaacagcct caaattaaaa tatttagcga agtcgaaccg aacccatcaa
     1921 ctaatacagt atataaaggt ctggaaatga tggttgattt ccaaccggat acaatcattg
     1981 cacttggtgg tggttcagcg atggatgctg caaaagcaat gtggatgttc tttgaacacc
     2041 ctgagacatc attcttcggt gctaaacaaa agttcctaga catcggtaaa cgtacttata
     2101 aaataggcat gcctgaaaat gcgacgttca tttgtatccc tacgacatca ggtacaggtt
     2161 cagaagtaac accatttgca gttatcacag atagtgaaac aaatgtaaaa tatccgttgg
     2221 ctgattttgc tttaacacct gacgttgcaa ttattgaccc tcaatttgtg atgagtgtgc
     2281 caaaaagcgt tacagcagat acaggaatgg atgtactaac gcatgcaatg gaatcatatg
     2341 tatctgtaat ggcttcagac tacacaagag gtttgagtct acaagcgatt aaattgacgt
     2401 tcgaatattt aaaatcatct gttgaaaagg gtgataaagt ttcaagagag aaaatgcata
     2461 acgcatcaac tttggctggt atggcatttg caaatgcatt cttaggcatt gcacactcaa
     2521 ttgcgcataa aattggtggc gaatatggta ttccgcatgg tagagcgaat gcgatattac
     2581 taccgcatat tatccgttat aatgccaaag acccgcaaaa acatgcatta ttccctaaat
     2641 atgagttctt cagagcagat acagattatg cagatattgc caaattctta ggattaaaag
     2701 gtaatacgac agaagcactc gtagaatcat tagctaaagc tgtctacgaa ttaggtcaat
     2761 cagtcggaat tgaaatgaat ttgaaatcac aaggtgtgtc tgaagaagaa ttaaatgagt
     2821 caattgatag aatggcagag ctcgcatttg aagatcaatg tacaactgct aatcctaaag
     2881 aagcactaat cagtgaaatc aaagatatca ttcaaacatc atatgattat aagcaataat
     2941 ctatctgata ataatcatct aactcacctg aaattacaaa agtaaaaaat gccacataaa
     3001 ctttaagtcg ataatcatta tacggttatc ggcttttatt tattgccaaa tcttcagaga
     3061 gatacaaact agacaatcat ttttttaaat aaagaaaata ttaagattga tactcatttc
     3121 acaaactatt actactttag agtataatta tttttaattt catataaata aaaaggcgaa
     3181 aataatgcgg tttaaaagta attaattgtt caaacgatat gtaatatgta aatactatat
     3241 atataatacc aattttaatg aaaattttta agggaggtaa ataatggaaa gtacattaga
     3301 attaacaaaa attaaagaag tattacaaaa aaacttgaag attttaatta ttttaccgct
     3361 attattttta attattagcg ctattgttac atttttcgtc ttatcaccta aatatcaagc
     3421 taatactcaa attttagtga atcaaactaa gggtgacaat cctcagttta tggcgcaaga
     3481 ggttcaaagt aatattcaac ttgtaaatac gtataaagaa attgttaaaa gtcctagaat
     3541 tttagatgag gtgtcaaagg acttaaatga taagtattca ccatctaaat tgtcgagtat
     3601 gttgacaatt acaaaccaag aaaatacgca acttatcaac atccaagtta aaagtggtca
     3661 taaacaagat tcggaaaaaa ttgcgaatag cttcgctaaa gttacaagta aacaaattcc
     3721 gaagattatg agtgtggata acgtatcaat tttatctaaa gcagacggta cagcagttaa
     3781 agtcgcacca aaaactgtag tgaatctaat cggtgcattc tttttaggat tagttgtcgc
     3841 gcttatatat atcttcttca aagtaatttt cgataagcga attaaagatg aagaagatgt
     3901 agagaaagaa ttaggattgc ctgtattggg ttcaattcaa aaatttaatt aaggatggtt
     3961 gctacttatg tcaaaaaagg aaaatacgac aacaacacta tttgtatatg aaaaaccaaa
     4021 atcaacaatt agtgaaaagt ttcgaggtat acgttcaaac atcatgtttt caaaagcaaa
     4081 tggtgaagta aagcgcttat tggttacttc tgaaaagcct ggtgcaggta aaagtacagt
     4141 tgtatcgaat gtagcgatta cttatgcaca agcaggctat aagacattag ttattgatgg
     4201 cgatatgcgt aagccaacac aaaactatat ttttaatgag caaaataata atggactatc
     4261 aagcttaatc attggtcgaa cgactatgtc agaagcaatt acgtcgacag aaattgaaaa
     4321 tttagatttg ctaacagctg gccctgtacc tccaaatcca tctgagttaa ttgggtctga
     4381 aaggttcaaa gaattagttg atctgtttaa taaacgttac gacattatta ttgtcgatac
     4441 accgccagtt aatactgtga ctgatgcaca actatatgcg cgtgctatta aagatagtct
     4501 gttagtaatt gatagtgaaa aaaatgataa aaatgaagtt aaaaaagcaa aagcacttat
     4561 ggaaaaagca ggcagtaaca ttctaggtgt cattttgaac aagacaaagg tcgataaatc
     4621 ttctagttat tatcactatt atggagatga ataagtatga ttgatattca taaccatata
     4681 ttgcctaata tcgatgacgg tccgacaaat gaaacagaga tgatggatct tttaaaacaa
     4741 gcgacaacac aaggtgttac agaaatcatt gtaacatcac atcacttaca tcctcgatat
     4801 accacaccta tagaaaaagt gaaatcatgt ttaaaccata ttgaaagctt agaggaagta
     4861 caagcactaa atctaaagtt ttattatggt caggaaataa gaattaccga tcaaatcctt
     4921 aatgatattg atcgaaaagt tattaacggt attaatgatt cacgctattt actaatagaa
     4981 tttccatcaa atgaagttcc acactatact gatcaattat ttttcgaatt acagagtaaa
     5041 ggctttgtac cgattattgc acatccagag cggaataaag caataagtca aaaccttgac
     5101 atactatacg atttaattaa caaaggtgct ttaagtcaag tgacaacggc gtcattagcg
     5161 ggtatttccg gtaaaaaaat tagaaaatta gcaattcaaa tgattgaaaa caatctgaca
     5221 catttcatcg gttcagatgc gcataacaca gaaatcagac cgttcttaat gaaagactta
     5281 tttaatgata agaaattacg tgattattat gaagatatga acggatttat tagtaatgcg
     5341 aagttagttg ttgatgataa aaaaattcct aaacgaatgc cacaacaaga ttataaacag
     5401 aaaagatggt ttgggttata aacagcaaat gaggggtttt atggcacatt tatctgtgaa
     5461 attgcggctt ttaatactag cattaatcga ttcactgata gtgacatttt cagtattcgt
     5521 aagttattac attttagaac cgtatttcaa aacatattct gtcaaattat taatattggc
     5581 agctatatca ctattcatat cgcatcatat ttcagcattt atttttaata tgtatcatcg
     5641 agcgtgggaa tatgccagtg tgagtgaatt gattttaatt gttaaagctg tgacgacatc
     5701 tatcgttatt acgatggtgg tcgtgacaat tgttacaggc aatagaccgt tttttagatt
     5761 gtatttaatt acttggatga tgcacttgat tttaataggt ggctcaaggt tattttggcg
     5821 tatttatcgg aaataccttg gaggtaagtc atttaataag aagccaactt tagttgttgg
     5881 tgctggtcaa gcaggttcaa tgctgattag acaaatgttg aaaagtgacg aaatgaaact
     5941 tgaaccggta ttagcagtcg atgatgacga acataaacgc aatatcacaa ttactgaggg
     6001 tgtaaaagtc caaggtaaaa ttgcggatat tccagaacta gtgaggaaat ataagattaa
     6061 aaaaatcatc attgcaattc caactattgg tcaagagcgt ttgaaagaaa ttaataatat
     6121 ttgccatatg gatggcgttg agttattgaa aatgccaaat atagaagacg tcatgtctgg
     6181 tgagttagaa gtgaaccaac ttaaaaaagt tgaagtagaa gatttactag gcagagatcc
     6241 tgttgaatta gatatggata tgatatcaaa tgaattgacg aataaaacta ttttagttac
     6301 gggtgcaggt ggttcaatag gatcagaaat ttgtagacaa gtttgtaatt tctatccaga
     6361 acgtattatt ctacttggcc atggtgaaaa cagtatttat ttaatcaatc gtgaattgcg
     6421 aaatcgcttc ggaaaaaaat gttgatatcg ttcctattat agcggatgtg caaaatagag
     6481 cgcgtatgtt tgaaattatg gaaacgtata aaccatacgc agtttatcat gcagcagcac
     6541 acaagcacgt gccgttaatg gaagacaacc ctgaagaagc agtacgtaat aatattttag
     6601 gtacgaaaaa tactgctgaa gctgctaaaa atgcagaggt aaagaaattc gttatgattt
     6661 ctacggataa agccgttaat ccgcctaatg tcatgggagc ttcaaagcga attgcagaaa
     6721 tgattattca aagtttaaat gatgaaacgc atcgaacaaa ttttgttgca gtgagatttg
     6781 gtaatgtact tggatcgaga ggatctgtga ttccactttt caaaagtcaa attgaagaag
     6841 gtgggccagt tactgtgaca catcctgaaa tgacacgtta ctttatgaca attcctgaag
     6901 cttctagact agttttgcag gcaggggcat tagcagaagg tggcgaagta tttgtgctag
     6961 atatgggaga accagtgaaa attgtagatt tggcacgtaa tttaattaag ctaagtggta
     7021 aaaaagaaga cgacatacgc attacttata cagggattag acccggcgaa aaaatgtttg
     7081 aagagcttat gaataaagat gaggttcatc ctgaacaagt atttgaaaaa atttatcgtg
     7141 gcaaagtaca acatatgaaa tgtaatgaag ttgaagcgat tattcaagac atcgtcaatg
     7201 actttagtaa agaaaaaatt attaactatg ccaatggcaa aaagggagat aattatgttc
     7261 gatgacaaaa ttttattaat tactgggggc acaggatcat tcggtaatgc tgttatgaaa
     7321 cagtttttag attctaatat taaagaaatt cgtatttttt cacgcgatga gaaaaaacaa
     7381 gatgacattc gaaaaaaata taataattca aaattaaagt tctacattgg tgatgtgcgt
     7441 gatagtcaaa gtgtagaaac agcaatgcga gatgtttatt acgtattcca tgcagcagct
     7501 ttaaaacaag tgccgtcatg tgaattcttt ccagttgagg cagtgaagac aaatattatt
     7561 ggtacagaaa atgtcttaca aagtgctatt catcaaaatg ttaaaaaagt catatgttta
     7621 tctacagata aggcagcgta tcctattaat gctatgggta tttcaaaagc aatgatggaa
     7681 aaagtattcg tagccaaatc aagaaatatt cgtagtgaac aaacgcttat ttgtggtaca
     7741 agatacggta atgtgatggc ttcaagagga tcagtaatac ctttgtttat cgacaaaatc
     7801 aaagctggag aacctttaac gattacagat cctgatatga caagattttt aatgagctta
     7861 gaagatgcgg tagaactagt tgttcatgca tttaagcatg cagagacagg agatattatg
     7921 gttcaaaaag caccaagctc aacggtaggg gatcttgcga ccgcattatt agaattgttt
     7981 gaagctgata atgcaattga aatcattggt acgcgacatg gagagaaaaa agcagaaaca
     8041 ttgttgacga gagaagaata cgcacaatgt gaagatatgg gtgattattt tagagtgccg
     8101 gcagactcca gagatttaaa ttatagtaat tatgttgaaa ccggtaacga aaagattacg
     8161 caatcttatg aatataactc cgataataca catattttaa cggtggaaga gataaaagaa
     8221 aaacttttaa cactagaata tgttagaaac gaattgaatg attataaagc ttcaatgaga
     8281 taggagagat tgacgttgaa tattgtaatt acaggagcaa aaggttttgt aggaaaaaac
     8341 ttgaaagcag atttaacttc aacgacagat catcatattt tcgaagtaca tcgacaaact
     8401 aaagaggaag aattagagtc agcattgttg aaagcagact ttgtcgtgca tttagcgggt
     8461 gttaatcgac ctgaacatga caaagaattc agcttaggaa acgtgagtta tttagatcat
     8521 gtacttgata tattaactag aaatacgaaa aagccagcga tattattatc gtcttcaata
     8581 caagcaacac aagataatcc ttatggtgag agtaagttgc aaggggaaca gctattaaga
     8641 gagtatgccg aagagtatgg caatacggtt tatatttatc gctggccaaa tttattcggc
     8701 aagtggtgta agccgaatta taactcagtg atagcaacat tttgttacaa aattgcacgt
     8761 aacgaagaga ttcaagttaa tgatcggaat gttgaactaa cgctaaacta cgtggatgat
     8821 atcgtcgctg aaataaagcg tgctattgaa ggaactccaa cgattgaaaa tggtgtacct
     8881 acagtaccaa acgtatttaa agtgacattg ggagaaattg tagatttatt atacaagttc
     8941 aaacagtcac gtctcgatcg aacattgccg aaattagata acttgtttga aaaagatttg
     9001 tatagtacgt atttaagcta tctacctagt acagacttta gttatccctt acttatgaat
     9061 gtggatgata ggggttcttt tacagaattt ataaaaacac cggatcgtgg tcaagtttct
     9121 gtaaatattt ctaaaccagg tattactaaa ggtaatcact ggcatcatac taaaaacgaa
     9181 aaatttctag tcgtatcagg taaaggggta attcgtttta gacatgttaa tgatgatgaa
     9241 atcattgaat attatgtttc tggcgacaaa ttagaagttg tagacatacc agtaggatac
     9301 acacataata ttgaaaattt aggcgacaca gatatggtaa ctattatgtg ggtgaatgaa
     9361 atgtttgatc caaatcagcc agatacgtat ttcttggagg tatagcgcat ggaaaaactg
     9421 aaattaatga caatagttgg tacaaggcct gaaatcattc gtttatcatc aacgattaaa
     9481 gcatgtgatc aatattttaa tcagatatta gtacacactg gtcaaaatta tgattataca
     9541 ttgaatcaaa ttttctttga tgatttggaa ttaagacaac cggaccacta cttagaggca
     9601 gttggaagta accttggaga aacgatgggg aatattattg cgaagacata tgatgtttta
     9661 ttacgcgaac aaccagatgc acttttaatt cttggtgata caaatagttg tttagcagca
     9721 gtatctgcta aacgattaaa gattcctgtg ttccacatgg aagcgggtaa tagatgcttt
     9781 gatcagaatg tacctgaaga aatcaatcgt aaaattgttg accatgtcag tgatgtgaat
     9841 ctaccttata cggaacatag cagacgttat ttattagatg aaggcctcaa taaagcgaat
     9901 atctttgtga caggatcacc gatgacagaa gtgatagaag cgcatcgaga taaaattaat
     9961 cacagtgacg ttttaaataa actaggatta gaaccgcaac aatacatttt agtatctgcg
    10021 catagagaag agaatatcga taatgaaaag aattttaaat cattaatgaa tgcgataaat
    10081 gatattgcca aaaagtataa aatgcctgtg atttattcaa cgcatccaag aagttggaag
    10141 aaaattgaag aaagtaaatt tgaatttgat ccattagtta aacagttaaa gccatttggt
    10201 ttctttgatt ataatgcatt gcaaaaagat gcatttgttg tgctatcaga tagtggaaca
    10261 ttgtcagaag agtcgtctat tttgaagttc cctggtgtcc ttattcgaac ttccacagaa
    10321 agaccggaag tactagataa aggtacggtt attgtaggtg gtattaccta taacaatcta
    10381 atccaatccg ttgaactagc aagagagatg caaaacaata acgaaccgat gattgatgct
    10441 attgattata aagacactaa cgtttcgaca aaggtagtta aaattattca aagctataaa
    10501 gatattatca atcgaaatac ttggaggaaa tgacgatgag gatagcgatt gaaaagataa
    10561 ttggtttgct gaaaaaccag tcctctaaag aatcgaatgt taagattcat cgcttggcgt
    10621 atattacaaa ctcaaaattt gatggcaata actatataga tagatggtgt aaaatcagga
    10681 attctcacat tggtgaatac agttatattg gatttggtag tgattttaat aatgtagaag
    10741 taggaagata ttgttcgata tcttcggatg taaaaattgg gttaggaaaa catcctacac
    10801 acttttttag ctcatcaccg attttttatt ctaataataa tccatttaac ataaagcaaa
    10861 agtttataga ctttaatgac caaccaagcc gtacaacaat taaaaatgat gtgtggattg
    10921 gtgcaaatgt aattattatg gatggtttaa caataaatac tggtgcagtc atagcagccg
    10981 gctcagttgt tactaaaaat gtaggagcat atgaggttgt tggtggtgtt cctgcaaaag
    11041 tgattaagaa gcgatttgac aataaaacaa ttgaaaaact tttggaaagc aagtggtggg
    11101 agaaaacgcc tgacaaacta aaaggatttt cggttgaata tttaaataaa aaggatactt
    11161 aatgatatga gaattttaaa tattgtatcg agtaatattg ttcaagaccc aagggtactt
    11221 aaacaaatag aaacaattaa aggcgttacg gatgattata aaattgttgg aatgaataat
    11281 tcacaagcta ctaataagcg attggaaaat ttagattgta attatcgttt gttaggtagc
    11341 aaggtagatc caaaaaatat tctttctaaa ttaattaagc gtataagatt tgcaacaggt
    11401 gttatccgag aaattaaagc ttataaacct gacgtgattc atgcaaatga tttcgacgta
    11461 ttattaatgg tctatttaag caattataaa aaagctaata ttgtttatga tgcgcatgaa
    11521 atatatgcga aaaatgcctt tattaataaa gttccactta tttcaaagtt tgtagaaagt
    11581 atagaaaaac acatagtaaa acatcgtgtt aatgccttcg taacagtaag tcatgcagca
    11641 aaagaatatt atcaatctaa aggatataag aaggaagcga atgttattac gaatgcacct
    11701 attttaaatg atagcagaga atttaaagaa atcgaaaact ttaaagaaat tgtatatcaa
    11761 ggtcaaattg taatggacag aggatatgaa gagtttatta ttgcttcatc agcttttaaa
    11821 caaaatgctc cttcattcat aattcgaggg tttggtccgc atgaagaagt gataaaagaa
    11881 ctgattagtt ataacccgga aaatattagg ttggataaac cagttgaagt aaaagaattg
    11941 gttgataagt tagcagaaag taatgttggt gttgtcttga cgaaaccagt atctattaat
    12001 tttgaatata cagtatctaa taaaattttt gaatgtatac atgctggttt accagtaatt
    12061 ttatctcctg tcaaagagca tatttatctc aatgaaaaat ataaatttgg cattgttcta
    12121 aaggaagtta cgccgttaga aattgaaaag gcggttagaa aattaagaga taatcacgat
    12181 ttgtttaatc atttacgtca aaatgcaatt aaggcgtcta aaattttgaa ttggcaaata
    12241 gagagtgaac ggttagtaga attatataaa ttttaaagag aggtagacta tgaaattttt
    12301 tgtactttgt gcaattatca gcatgaacat atttatagta atctctacat ttactaaaga
    12361 agtattaggg ttccctatag agccggtgta ttactcaacc atggttggta tagcattaat
    12421 tactacggtg tttgctattt ataagataat tgtcacccaa gaaattccgc gagggttaat
    12481 attattaatt gctatatgtt tgctttatct agctttttat tatttttcac cagataagga
    12541 agagaaacta gctaaaaata atattctatt ctttttaaca tgggcagttc cagcggcaat
    12601 tagtggtatt tatattaaat atataaacaa ggctacggta gaaagatttt ttaaattagt
    12661 atttttcata ttttctgttt catttatttt tgtaatttta ataccaaaac ttacaggtga
    12721 gatacctagc tatatcaatt ttggacttat gaactatcaa aacgcttcgt acctttcagc
    12781 atttactgcc ggattaggca tttatttcat tatgaaaggt tcagttaaac ataagtggat
    12841 atatgttcta tttacaataa ttgatatccc tattgtgttt ataccaggag ggcgtggagg
    12901 tgctatttta ttaattcttt acggcttatt tgcatttata cttattacgt ttaaaagagg
    12961 aatacctatc gcagtaaaaa gcattatgta tatttttgca ttaagcatat ctagtgtatt
    13021 gatttacttt ctttttacaa aaggttcgaa tactagaaca ttttcatatc tacaaggtgg
    13081 aacacttaat ttagaaggta cttctggaag aggaccgatt tatgaaaaag gtatttactt
    13141 tattcaacaa agttcgttat taggctatgg gccatttaac tattataaac taatcggaaa
    13201 tataccacat aacatcatta ttgagttgat tctatcattt ggcttattag ggttttttat
    13261 cataatgatt tgcattttgc tactagttta taaaatgatt aggaactatg atccaaacac
    13321 tatagattta ctcgttatgt ttatagcaat ctatccaatc acattattaa tgtttagttc
    13381 aaattattta gttgtaagtg aattttggtt tgtgttgttc tattttatta caaaaggacg
    13441 gcgtcatcat ggctaagaaa gtttttatta tggatagcgt aaagacaata attggtacgt
    13501 tgcttatagc tttaggatta caatttttag cttatccaat tattaatcaa cgagtaggta
    13561 atgaagcgtt cggttctatt ttaacgattt atacaataat aacaatcacg agtgttgtat
    13621 taggcaatac gcttaacaat atacgattga ttaatatgaa tctatacaaa tccaatcatt
    13681 actactggaa atttgcatcg atacttttaa tctcaattct gattgagagt atagctttaa
    13741 ttattgtatt tctttacttt tttaatttga acatcatcga tat
//
LOCUS       DAMQYT010000055        13568 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMQYT010000055 DAMQYT010000000
VERSION     DAMQYT010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13568
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.055"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(112..684)
                     /gene="raiA"
                     /locus_tag="PGA33_001217"
     CDS             complement(112..684)
                     /gene="raiA"
                     /locus_tag="PGA33_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDG6473038.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            complement(745..1419)
                     /locus_tag="PGA33_001218"
     CDS             complement(745..1419)
                     /locus_tag="PGA33_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDG6473039.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKHLNQDEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFLKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            complement(1412..2494)
                     /locus_tag="PGA33_001219"
     CDS             complement(1412..2494)
                     /locus_tag="PGA33_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDG6473040.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRK
                     RQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNKKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     SMNMIQAKKEIQRMNKLALKRGWIDE"
     gene            complement(2858..3724)
                     /gene="fakB1"
                     /locus_tag="PGA33_001220"
     CDS             complement(2858..3724)
                     /gene="fakB1"
                     /locus_tag="PGA33_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HDG6473041.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            3868..4509
                     /locus_tag="PGA33_001221"
     CDS             3868..4509
                     /locus_tag="PGA33_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDG6473042.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDIGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            complement(4679..5734)
                     /locus_tag="PGA33_001222"
     CDS             complement(4679..5734)
                     /locus_tag="PGA33_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDG6473043.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
     gene            6052..7122
                     /locus_tag="PGA33_001223"
     CDS             6052..7122
                     /locus_tag="PGA33_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDG6473044.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            7318..8079
                     /locus_tag="PGA33_001224"
     CDS             7318..8079
                     /locus_tag="PGA33_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDG6473045.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            8097..8591
                     /locus_tag="PGA33_001225"
     CDS             8097..8591
                     /locus_tag="PGA33_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDG6473046.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            8605..9831
                     /gene="pepT"
                     /locus_tag="PGA33_001226"
     CDS             8605..9831
                     /gene="pepT"
                     /locus_tag="PGA33_001226"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDG6473047.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(10020..11144)
                     /locus_tag="PGA33_001227"
     CDS             complement(10020..11144)
                     /locus_tag="PGA33_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDG6473048.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAYQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(11569..11889)
                     /gene="ytxJ"
                     /locus_tag="PGA33_001228"
     CDS             complement(11569..11889)
                     /gene="ytxJ"
                     /locus_tag="PGA33_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HDG6473049.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            complement(12043..12921)
                     /locus_tag="PGA33_001229"
     CDS             complement(12043..12921)
                     /locus_tag="PGA33_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HDG6473050.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            13041..13559
                     /locus_tag="PGA33_001230"
     CDS             13041..13559
                     /locus_tag="PGA33_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDG6473051.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYTKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
BASE COUNT         4489 a         2334 c         1937 g         4808 t
ORIGIN      
        1 gtcactatgt ttcattcaac aaattacatt atcgaactat gaaatagtca taatttgctt
       61 ttggagtata aaaaagcact tgtgcaaaaa cacaagtgct ttaaacttaa tttattgttc
      121 actagtttga atcaagccat atttaccgtc tttacggcgg taaacgatac ttgttccatc
      181 agtttctctg tctgtgaata caaagaagtc atgacctaat agattcattt gtaataccgc
      241 ttcttctgaa tccattggtt ttaagctgaa ttcttttgaa cgaataattt ctatctcgtt
      301 atcatcgtaa gcgtcattat caacttgtgt ttcttgcatt tcttgtaatt cggcaacaaa
      361 cacttcttga tctcctcgat cacggctctt acgattaata cgtgttttat attttcgaac
      421 ttgtctttca agtttattat taattaaatc aatacctgcg tataaatcat cgtttcgctc
      481 ttcagctctt aacgtaacat ttttcaatgg aattgttact tcaattttag tagctgaatt
      541 tgaataagtt ttaactttaa catgcgccac tgcatttggt acgtcattaa aataacgttc
      601 caacttacca attttttcct caatatagtt gcgaatagca tctgtgatag tgaggttatc
      661 tccatgaatt tcaaatctaa tcatagtaat ctctccttaa acctctttat tggtaactct
      721 ttattatatt ttaacatttt tacgctatcg tgcaaacgca aacactttga attttctgat
      781 atttttagca tataatttac accctgcacg atgaattgtt aatccagttg tataaatatc
      841 gtcaacgagt aatatttcct taccatttaa atctaattcc gtatcaataa taaatggatt
      901 ttcatctgcc agacgctctt tcttagttaa atgagactgt tttggtctat ttgacatctt
      961 taaaatctta tcaaagcgaa tccctttagc ttttagtact gcttctaccg ggttaaatgt
     1021 tctagataaa tcatgtgccg gcgaagaagg aattggcaca atatagtcat aagatgtttg
     1081 tggtatttca atcaaatgtg ccaataattc acataaataa tagtctttca aaaatttata
     1141 ctgatgtatc atctctttca ttaaaccgtc atattgaaat tgacaatata attgttccat
     1201 taaattaaag tgtgccgata gaaacttgca gtctaaacaa tacgcttcat cttgatttaa
     1261 gtgttttaag caccttgaac atcgccttgc tttaatatca agtttaatat tgtcccaatt
     1321 ctctttgcat ctgtcacata atctattagg tttcttgaac aaattataaa tggttatatt
     1381 ttcatataac ttagcaccac aactcaaaca attattcatc aatccaacct ctttttaatg
     1441 ctaatttgtt catcctttga atctcttttt tagcttgaat catattcata cttacccctt
     1501 catgaaaaaa caatactttt ccagtaggac attctaattt tcgtccaaca cgtccagcaa
     1561 tttgtattaa agcctcttga gtatattgat gtgcatcgat aacaacaaca tccaaatttg
     1621 ccattgtaaa tccacgttct aatattgtcg tagtaaaaat gacatcgaaa tgtccattcc
     1681 ttaattgttc aactttttca aagcgaaaaa catcctcgct atggacgtat gttaatttag
     1741 taattttctg cttataaacc gaaaatgttt taatcattgt ttctatattg ttaaaaaaca
     1801 ccagtgtata acgttgatta ttaatttgat cttgtaaaat tcggtataac attttctgaa
     1861 tcttcttatt attaagtttg aaataacgat atttaggaac tggaagtgat tttttatgaa
     1921 agcgagctgg caatttaatt atattttcgt ggggaatctc tgacagaagt tgtttcggtg
     1981 gtgttgctgt catataaatt gttgcatgtt caactttaga agatgacttc aatgcttgtt
     2041 gtaaattttt atccattgat aaaggaaagg catcgacttc atcaataaaa atagtatcaa
     2101 agtgctgttt gaatcggtaa agttgatgca ctgtgcatac aacaaaatgc ccttcaaatt
     2161 gttgtcttga ttgctggtgt agtatgtcta tatcttcatt aagaaatgcg tctttaatac
     2221 gtttactaat ttctacaaca acatctacac gtggtgacac aatagctata ttatctccct
     2281 gtattcttgc atattgaatg ccttgaaaca tcatttctgt cttacctgca cctgttacgg
     2341 catataacaa aatcgtttgt ctctttctaa tggctcgaac aatatgttct gaggcataag
     2401 actgctgttc cgacaattca aatgagagat gataatatgc ttgtgatgaa ctttgcgaac
     2461 tctctgttat tttatatctt gttacattat ccattcgacc cagtgatata caatttctgc
     2521 aatatacaat tttcttggaa tgtactgagg aatcatactg ataaaaatgc tttggatttg
     2581 tactttcaca ttgaacacaa taatagtttg aattttgttt gatgacacct gttacttctt
     2641 ttttaatttt ttcattagta agtgaagtat gaggatgaat taatttcccg taatgtttaa
     2701 tgcttaaaac ctcatttcaa cgtaaaaaag agcctaatat tataatgatt ttcttatatc
     2761 tttagcattt tggctttcaa atgatgaaca tttaaagcca tgtatacgag agataatttc
     2821 caattacaat atagactctt ttattaaaat tttataatta tgttaatctt atttttctgc
     2881 caacatagcc taaacctaat ccaccagaac ctaaatgtgc tgcaacaact ggaccaaact
     2941 cagagtatgc tacttgataa gctgaaggac aatcatcttg taactttttg tataacgctt
     3001 gaccatcttc gaaatgatct ccatttatga caaataaagt tacttcttca aagtctttta
     3061 caatatctaa tactttcttt tctaatgttt gaatggcacg ctttttagta cgaacttttt
     3121 cttctggtat aatcttgcca tcttcaaact taagaactgg cttcattttc aataatgttc
     3181 caacccatgc ttgagcacca gtaattcgac cacttttttg taaattcttt aagtcatcaa
     3241 caattaaata tgcacctgtg tgttcacgca tattagttaa atcatcaata atttgctgtg
     3301 gctcgtatcc ttcttcaacc atttcaatag cacgtaatac ataacatcct tcaatcattg
     3361 ctgcaagctt actatcaaat gcatgtacat taacaccttc aaccatttcc cctgcttgat
     3421 aactagattg ataacttcca ctaatcccac ttgataagca aatgacaatg atatctgtgt
     3481 aaccttgatc tcttaacatt tcatatttag taatccattc gccaattgct ggttggcttg
     3541 ttgttggaat cgtttgagac gatgccattt tattataaaa ttcttcaatt gctatttcat
     3601 tactttctgt aaagttcttc ccatcatcaa aagtcacact taatggcgct atttgaatat
     3661 tatatttatc gattaagtcc tgcgacagat aacttgtaga atcggtcatc acagcaattt
     3721 tcatagtcgt cctcctaaaa tatactcata gcaatgatac aatatttcag ttaaaaagaa
     3781 aatggcaatc aaacgattta tagtcatgtc attttcagtg tataatgaag attaaataat
     3841 cttaagctta tgaattaagg agttattatg acacaaaata tcattactgt taaaaaagaa
     3901 cacattattg aaaatgtgat tagtaaatca agattcattg ctcatatcaa acctgttcaa
     3961 aatgaagatg aagctaaagc atttattgct gcaataaaaa aagaacataa agatgccact
     4021 cataattgtt ctgcatatac tattggacct gaaatgaata ttcaaaaagc gaatgatgat
     4081 ggtgaaccga caggaactgc cggtgtccca atgttagaca tattaaaaaa gctcgatgtt
     4141 cataatgcgt gtgtcgtagt aacaagatat tttggtggta ttaaattagg tggtggcggt
     4201 ttaattcgcg cctatagcgg tgctgttaga gacgtcattt atgatatcgg gcgtgtcgaa
     4261 ctacgagaag cagtaccatg tatagtaaca ctgaattatg atcaaactgg taaatttgaa
     4321 tatgaattag cttcaactca ttttatgtta agaaaccaaa cttacactga taaggtaagc
     4381 tactatatcg atgtcgttaa aagtgactat gaagcgttta ttaacttttt aaatcaaatg
     4441 actgcaggaa atttcgattt gactgaagaa actccaaagc agttaccttt cgatatacca
     4501 actgaataaa aataaaaagc actatcataa gacggtaaat atcaattgcg tattattaaa
     4561 cgacaattga agtacacgtt tatggcagtg ctttttatat ttgaaattta atacaaaaaa
     4621 attgtaaaga gtaaagccat ataatacaaa ttatcccata cagctatgct ttcattccct
     4681 attcctcttt atgagatgac ttacgactaa ttaaatttaa tattggtcgg tagttgttat
     4741 ctattaatcc tgtaaattca acaattaatt caatactaaa tatgattaat acaaacatta
     4801 atacaacacc taatggtggc gatacataca aaataatgct cgaaagacta aataaaatag
     4861 agattgaata gattaataat actgtttgtc tatgtgtgta gcctaaagct aatagtttat
     4921 gatgcaaatg cgatttatca gcttgcatta tatgctgccc ttttttcaca cgtcgaatca
     4981 ttgcgaacaa agtatcaatg aatggaactg ctaagataac aattgggaag aacaatgcaa
     5041 taattgtaat atttttgaat ccgagtaaag aaaggaatcc gatgataaat ccaatcatta
     5101 aagccccact atcacctaaa aatattttgg caggatggaa attgtaaaat aaaaacccaa
     5161 ttaaagagcc taataaaaca caacaaatca tcgttatgaa aatattagct tgtaaaattg
     5221 caatgaaccc tattgtaatg agtccgattg cagaaacacc cgacgccaaa ccatcgagtc
     5281 catcaattaa gttaattgca tttgtaatag caacaatcca aatcacagta attggaatac
     5341 taagaaatcc aaaatgaatc gttgtaccca ttggcaacga aataaaatca atagtcacac
     5401 cataaaaagc aactactaag gcagcggcaa tttgaccagc caattttata tacggtttca
     5461 aatcgtagat atcatctaca agcccaagta cgtacataat aatcgcacca ataataagtg
     5521 gtttgatttc tgtttcaata ggatgaccaa tccaaatacc tattaaaaat gaaaagagaa
     5581 tcactgtacc acccataact gaaataggtt tagtgtgtac ttttctaaaa tttggtttat
     5641 caactaaatt taatcttttc gatattgcaa taacaattgg tgttatcgtc aaactgacaa
     5701 tcattgttac tgcaactagt aataatgtaa ccatttattc accttcatcg atattaattg
     5761 tttttaacgt tatatttatt taatattgtc tttatagaat gatatatcta ttttctacgt
     5821 atatcttata gctttattat aaattaattc gttcaatcaa tgatatcata ttaacaacta
     5881 tacgattcat ttcttatata tcgcctatta ttcatttttt catcatatca tttaaggcaa
     5941 cattttaaca tcaaagtcta tatcatacat taattttata tgtaaactaa tgacaaattg
     6001 taagttttca cttacaatag ctagtggtca attaaaggag tcgattcaga catgttcgaa
     6061 gcatttatat acaatatatc tgttatagtc gctggaatct atttattcca tcgcttacag
     6121 tattcagaaa acaaacgtat ggttttttct aaggcttatg tcactgtttt aatgacaatc
     6181 gtatcattat tattatctgt ctaccctatc ccttatcgtg aagattattt aattcactta
     6241 acattcgttc ctttattgtt tttaggtaga tttacaaata tggtctatac attatctgcc
     6301 acagtaattg tagcaattgt tgaaattgta gtatttaaca actcgattat gtacggtgtt
     6361 acgttgattg ttattgctgc agtaacaagt gctattggac catttttaaa acaaaatgat
     6421 gtgctatcat tattaatact taatgttgtt acaataatca ttttatttgg tgtcgcgtta
     6481 gtgagtccta tttacacact atcggaagtt atcattttaa taccaatttc attaattatc
     6541 actttagctt cagcgataac atttgtcgat atttggcatt tcttctcact cgtaaatcgt
     6601 tatgaaaacg aagataaata tgattactta acaggcttag gtaatgtaaa agaatttgat
     6661 agacatttaa atgaaatttc acgaaaagct gaaaaagaac atcaaagtat cgcattatta
     6721 ttaatcgata tcgatggatt taaagatgtc aatgatacct actcacacaa atcaggtgat
     6781 gctgtattaa aacaaatgtc tcaattactt aaaaactatg tgccgaatca atttaaaatt
     6841 tttagaaatg gtggcgaaga gttctcagtt gtcattcaca attattcact tgatcaaagt
     6901 gttaaattag ctgaaaatat tcgttcgggt gttgaaaaat catcattcca tttaccaaac
     6961 aaagaagtta ttaaattgtc agtgtctatc ggtgttggtt atttaacaga tgacgatcca
     7021 aaatcacagc gtaaagtgtt taaagatgca gacgatatgg tgcatgtagc caaaaatcaa
     7081 gggcgaaaca aagtaatgtt taaccctatt atcaatttat aacaacgaca aaactgttaa
     7141 tcctttgcta ttattaaatc atagtataat agcaaagatt aacagttttt cttatttaaa
     7201 tttgaaccat acaagttcat catactaaca caaagtctca ctattgaaag ttgtaacaac
     7261 aagttgtaaa cttcaattgt tcaaataaat aacacatatc attggaggtg aattgatatg
     7321 ccagattcaa tcacaattat agatgaaaac aaagtgattg atgttgtatt aattgcaggt
     7381 agaattttac ttgaatcagg tgctgaaaca tatcgagttg aagatacaat gaaccgtatc
     7441 gcacatagtt atggtcttca taatacatat agttttgtca gttcaactgc aattattttt
     7501 tcattaaacg atcgaacaag tacaagatta attcgtgtac aagagcgtac aacagattta
     7561 gagaaaatcg ctttaacgaa tagtctttca cgtaaaatat caaataaaga actcacaatt
     7621 gatgaagcta aatctgaatt tattcattta cagcatgcat cattacaata ttctttttta
     7681 acgaatttct ttgcagctgc cattgcatgt ggcttcttcc tatttatgtt tggtggtgtc
     7741 gcatcagatt gttggattgc agtcattgct ggcggatccg catttttaac attcagcttt
     7801 gtgcaacgct atatacaaat taaatttttc tcagagtttg ttgcagccgc tgtggtcata
     7861 tccattgcag ccacatttac taaattaggc attgcaacca atcaagatat cattactata
     7921 gccagtgtca tgccacttgt ccctggtata ttaattacca atgccattcg agatttatta
     7981 gcgggtgaat tacttgcagg tatgtctcgc ggtgttgaag ctgcattaac agcattcgca
     8041 atcggtgctg gtgtcgcaat cgttttatta atcatttaag gaaggactgt taaagcatgc
     8101 tattttattt atttcatttt acaatcagct ttatatcaac agtacttttc tctatcattt
     8161 tcaatgcacc caaacgcctc ttagtagcat gtggatttgt gggtgccatt gcatggacga
     8221 tttaccaatt aacggtagat ttagagtttg gaaaagttgg cgcttcattt ttgggaagct
     8281 taattttagg cttaatgagt catactatga gtcgcagata taaacgaccg gtaattatat
     8341 tcatagtgcc aggcattata ccattagtac ctggtggtgc agcttatcaa gcgactcgtt
     8401 ttttagtatc aaatgattat acaagtgctg taaatacatt tttagaagtt acactgattt
     8461 caggtgcgat tgctttcggt atattagttt ctgaaattct atattaccta tacacacgta
     8521 tcaaacaact gtatggtaaa atcaaaggta agacatataa aaaatcttac aacatgaata
     8581 atagagttta aggggtcgaa tataatgaag aaccaattga tagatagatt aacaagatat
     8641 acgacaattg atacgcaatc tgatccaaaa tctacaacaa caccttcaac tgaaaagcaa
     8701 tgggatttgt tacatttatt agaaaaagaa ttacaacaat taggattgcc aactgattta
     8761 gatgaaaatg gctacttatt tgctacatta gaaagtaaca tcgatgtcga cgtcccaaca
     8821 gttggatttt tggcgcatgt tgatacatca cctgatttca atgcttcaaa tgttaaaccg
     8881 caaattattg aaaactatga tggtaagcca tacaaactag gcaatactaa acgtgtctta
     8941 gatcctaaag tatttccaga acttaacagt ttagttggtc atacattaat ggtaactgat
     9001 ggtacatcgt tactaggtgc agacgataaa gcgggtattg tggaaattat ggaagctatt
     9061 tgttatttac aagaacatcc agaaattaaa catggtacca ttcgcattgg atttacacca
     9121 gacgaagaaa tcggtcgtgg tccacataaa tttgatgttg accgcttcaa tgctgatttt
     9181 gcttatacta tggatggtag tcaatatgga gaattacaat atgaaagctt taacgctgct
     9241 gaagcggtta ttacatgcca cggtgtaaat gttcatcctg gttcagctaa aaatgcaatg
     9301 gtaaacgcaa tacgtttagg tgaacaattc gatagtttgc tacctgatag tgaagttccg
     9361 gagcgaacag aaggatacga aggcttttat cacttaatga actttgaagg aactgttgaa
     9421 aaagcaactt tgcaatacat tattcgtgat catgataaaa aacaattcga attgcgtaag
     9481 aaacgtattt tagaaatacg tgacgatatc aatgcccatt ttgaaaatta tccagttaaa
     9541 gttgatatat cggatcaata tttcaatatg gcagaaaaaa tattaccatt gcctcatatt
     9601 attgatatac ctaaacgtgt ctttgccaaa ttagatattc cagcaaacac tgaacctatt
     9661 cgcggtggta cagatggttc acaattgtca tttatggggt taccaactcc aaacatcttt
     9721 acaggttgcg gtaacttcca cggcccatat gaatatgcat ccattgatgt tatggaaaaa
     9781 gcagtacaag taatcattgg aattgtagaa gatatcgctg aaaatcacta aaaaccaacg
     9841 attatatact agttcaactc aggcttaatt aatatataaa aatcacgcta acattcagtg
     9901 caatgatagc gtgatttttc atatttttta cgtatacttt cgatcttcta attcgttttg
     9961 ctaaccatca taaatgttat taaccatcat gacagcatca ccaatttagt ccttctttac
    10021 taaacctcaa cattaaaatg tgttttcaac agttttaaag actgaaccgt ataatgccta
    10081 atttgtaacc ccattttaaa gtcagtataa gtttctggca tatcgataaa tgtattaaac
    10141 attgctgtaa ccccttgtga ttcaaataaa tcaaactttt cagcagttgc acaaattgca
    10201 atggcaacct tttgatgttt atgacaaagt tctgcaatac gcaatgttgt cgtttctagc
    10261 aactgatcat tttcatttaa tccttctcca aaaataatta aatccgcttg ttcaactaaa
    10321 ttttctaaat gtgttagttg gtctactaat gcatgactgg ttaatatttc agcttgatac
    10381 agtccattca agactgctgc aattccacca ccagcaccac cacgttcaac tggaccaatt
    10441 gcaattttca attcactttt aaataactca ctaaaatacc aaattaaatt atcgatttct
    10501 gctgcttgat tatgattcaa ctgatacgca tcataagttt gcatgatttc actttgctta
    10561 ccatataatc gacttgaaaa atccgacatt acttgaattc ttgctgtttc cattttaggg
    10621 tgtaagttcg acatatccat acgacgaata tatttaatta caccagcacc ttgtctcata
    10681 tctacgacac gcccttcgtc atcatagaat tgagcaccta atgcttgtaa catacctgca
    10741 ccagcatcaa aactatcaat cccacctagt gaaattacaa catgttttgc gtcgttatct
    10801 aatgcatgtt taatcatttc tcctaaacca taacttgttc gttcaacaat tggtttttta
    10861 ccttttaaaa ataaattccc ctcgataacg gtcatccctg tatcagtttg tccgtaaaca
    10921 ccttcaactt cattcatatc tgcatcatgt actggtatac gatacttttg cccagattgc
    10981 cataaaaata cagaatctaa taattcatgt cttccattaa acaatggtac ttgaactaca
    11041 tctgcagttt caatttggct tgcaactgcc tcttcaacat atctattagc ttgataactt
    11101 gaaataattc catgaaactc atccatggct actaatactt tcataacctt gaccacctta
    11161 ctttattatt tgttgtgata cgttttttat tcataaaaga ttaatcttaa attttagatt
    11221 ttatctcgtg attatatgcg aacatttaac atgacatgat tattaattat tccagtaaat
    11281 aacaatatgc ttcttttggt ttagcatata tttcctatta aactaactta caaatcatgt
    11341 ttaaattttc ttattggatt cgtcgttttc caaaatattt tcaataaaaa tactcgaata
    11401 tattatacat atgtttgtaa aaaagtacta acttaactgc ttctaaaggt gacataaaat
    11461 aaaaggtcag aacatcatta tagccaaact actctgattc acacgaattt aagttgtgct
    11521 attcacgtct agtagtgtaa ggcaaaatgt tccaacccta tagtttcatt attcttctgc
    11581 ttgtgctaat gacgacacat tgatatcacc gtggtctcga ttccaaacca tttcaccatt
    11641 tacaaaataa aatgcttgag gtgattcatg tttaacgttc gtttttttag caatataatc
    11701 tgacaaatcg cgttcttgtt ggacaatcaa ataataaccg tccatatcgc gttcatataa
    11761 aaatttatta aattgatcgt acgcatttgc cgatattgga caagtttcac tatgttttaa
    11821 tacaaaaaca tatttatttt cctcaataac ctgttcaaat tggtcaattg aacttagctt
    11881 tatagccact ctattcacct ctaaaaatat ttatcacatt taaatagcat ctgcgtgaca
    11941 tttttataca tatagttaat atcaatgttt accattatac acatagtaag ttgacatgtt
    12001 aaccataaat tcaaaatttt taactttgaa aaatgattat agttattttt cttctaattt
    12061 ttcaagcttt ttttcaaagg ctttatcgcc gtgagttata tctataccct ttttcggcaa
    12121 cttatctaca tcgacttttg ataacttctc actaaccttt attgtttcaa tacgtgtatt
    12181 ataatagttc tgcagactgt aatacatttc tattgcgttt ttccttgcat tattaacatg
    12241 cttatcacta atattagttt ggttaatatc ggtaatttgc ttttcaatca ttggcataat
    12301 gtgattttta atcgcgcctt ttacttcatt ttctttacta tcatctaggt tcttcttcgc
    12361 agtatctctt aacgctttat tattatgttc taattttgtc gtaagattag ttgcttcact
    12421 tttattatca gctaatttaa tttctgattc aactttgtat ctattttttt caaattgttt
    12481 cgtataatct aaaatgtctt cgttatactt gatagattga ttacataaac ctatgaattt
    12541 ttttaattga tatatcgcat ccttcttctc atttgcaagc gtcatatatt ctttttttaa
    12601 tttcttaact ttcattgtat catcaggcaa attttttgct gacttataat atgcttcaaa
    12661 tttaggtatg agatggtttt taacatcttc ttgtagtgcc ttaaattctt tactattttt
    12721 atcagttgta tcagttttac ttagatgatc aatttctttc aaatgtatat tatccatgac
    12781 atgttgtaat tctttttgtt tttgctcaac atctttaaat tgcttagtaa attgatgttg
    12841 tgcctctttt tctttattac cacaagcact tattactaca aataaaatcg ctaaaacagc
    12901 gataataaca atttttttca tgattgcact ccttaacaga ttcattatac aaaaacatgt
    12961 ttattatgaa aatgaattat gttatagtat cccagacttt atcgtgatta aattaatttt
    13021 caatatcaaa ggagtttatc atgtattcaa atgttcaacc attcattaat agtgatttaa
    13081 ctaattgttt tcaatcacaa tacactgaaa ttgaatcatt attattcaac ctcttagaca
    13141 gcccagtaaa atatacaaaa catattggcg gtacgcatca ttttaaatat tcaacagagc
    13201 caattttaga tattttagta ggtgtagaaa atttgcatga tattacatcc ttagatgaaa
    13261 aacgcttaaa ttatgtaggg ttttatcgtt tgcatcataa atatactaag aaagttatga
    13321 tggcgaagtt taacaatttt catgaactaa aacaacaggt gcgtctacat atcattcaaa
    13381 tgaataccca aacatttgaa tcttatcttg cacttgatta cctcttatca actaatgatg
    13441 atattgcttt gcaattcaat acaaaaaaac accaaatatt gcaacatagc ttaacaatta
    13501 gacaatatga gatgcaaaag caacaatact ttgaaaaact atgtaaaaaa tacttataac
    13561 gacaataa
//
LOCUS       DAMQYT010000056        13532 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMQYT010000056 DAMQYT010000000
VERSION     DAMQYT010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13532
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.056"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..208
                     /locus_tag="PGA33_001231"
     CDS             <1..208
                     /locus_tag="PGA33_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDG6473052.1"
                     /translation="TNDDYNVKQLRKRYDIPTNKAPKLLLKGTGNLKGSSVGYKKIEF
                     TFLENKNENIYFTDSLHLEPSEDK"
     gene            272..1042
                     /locus_tag="PGA33_001232"
     CDS             272..1042
                     /locus_tag="PGA33_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDG6473053.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAKIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMATQNKGEALKVKGMVLYMNRNTKTT
                     KGYYYVNAIKNDKDGRPQENEKRYPVKMVDNKIIPTKEIKDKNIKKEIENFKFFVQYG
                     NFKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKDIEFTFVEKKEENIYFSDGLIFKPSEDK"
     gene            1109..1876
                     /locus_tag="PGA33_001233"
     CDS             1109..1876
                     /locus_tag="PGA33_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDG6473054.1"
                     /translation="MKRLNKLVLYISFLILVISFTAGCGIGKEAEVKKSFEKTLSMYP
                     IKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMVVQPKGERMKSKGMVLYMNRNTKTTT
                     GKYIVSETLHDEDGRPKSKDKEYPVKMVDNKIIPTKGIKDENIKKEIENFKFFAQYGS
                     FKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYKIPTNKAPKLLLKGSGD
                     LKGSSVGYKDIEFTFVEKKGENTFFTDSLHLEPSEDK"
     gene            1970..4207
                     /locus_tag="PGA33_001234"
     CDS             1970..4207
                     /locus_tag="PGA33_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDG6473055.1"
                     /translation="MQRDYLIRVETESMSDFKRLNSLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDVHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFHHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHDYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFQPTNHQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFQVNEVLAYDVIPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLDQYDSRDYIDAFKIFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHRLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCKNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEEGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            4358..5536
                     /locus_tag="PGA33_001235"
     CDS             4358..5536
                     /locus_tag="PGA33_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDG6473056.1"
                     /translation="MNQQLIETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKTMIENGVLDGVDHV
                     LGVHVMSTMKTGKVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEDMYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEMCEPQPPSED
                     FAYYAKERPSAFIYTGAAVENGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            5538..6926
                     /locus_tag="PGA33_001236"
     CDS             5538..6926
                     /locus_tag="PGA33_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDG6473057.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGTDM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRVIQGVSAACIMPSTLAIMKTYYQGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEITNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLNVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(7419..9080)
                     /locus_tag="PGA33_001237"
     CDS             complement(7419..9080)
                     /locus_tag="PGA33_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDG6473058.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDLWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(9406..11181)
                     /locus_tag="PGA33_001238"
     CDS             complement(9406..11181)
                     /locus_tag="PGA33_001238"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDG6473059.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHISGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKIDVTTSQKIAREILIDRNGNAE
                     SIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFT
                     INRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSPFDLRVLMDAIYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHKLL"
     gene            complement(11355..12227)
                     /locus_tag="PGA33_001239"
     CDS             complement(11355..12227)
                     /locus_tag="PGA33_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDG6473060.1"
                     /translation="MNKLQDTTFLSYLFTIILWGSAFPMIKIALNDFSAESLSAFRLI
                     LATIILLPFVIIKKLPTPELRDIPVIFILGFCGFVIYHTALNFGEALISAGISGILVS
                     TTPIFSSALAYIFLKEHFSKWNWLSSLVAFIGISIISISKDDYTTINVLGVFIILLAS
                     FSESLYFTFQKKYIEKYGFIAFTLYTIMASSPFMLIFIPEIINDIHGATFTSIVSVLY
                     LAIFPTIIPYVLLAYIVKSVGVSDATMSLYLTPIVSLLLSYLLLDELPTTLAIIGGII
                     TLLGVSLSNFFQNT"
     gene            12340..>13532
                     /locus_tag="PGA33_001240"
     CDS             12340..>13532
                     /locus_tag="PGA33_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDG6473061.1"
                     /translation="MAKYKDIASDIRDKIITGDWFYGMKIPSHRQLAIQYNVNRVTII
                     KSIELLEAEGFIYTKVGSGTYVNDYLNEAHITNKWSEMMLWSSQQRSQYTVQLINKIE
                     TDDSYIHISKGELGISLMPHIQLKKAMSNTASHIEDLSFGYNNGYGYIKLRDIIVERM
                     SKQGINVGRENVMITSGALHAIQLLSIGFLGQDAIIISNTPSYIHSTNVFEQLNFRHI
                     DVPYNQINEIDTIIDRFINFKNKAIYIEPRFNNPTGRSLTNEQKKNIITYSERHNIPI
                     IEDDIFRDIFFSDPTPSIKTYDKLGKVIHISSFSKTIAPAIRIGWIVASEKIIEQLAD
                     VRMQIDYGSSILSQMVVYEMLKNKSYDKHLVKLRYVLKDKRDFMLNILNNLFKDIAHW
                     EVPSGG"
BASE COUNT         4712 a         2057 c         2442 g         4321 t
ORIGIN      
        1 aactaatgat gattataatg taaaacaatt acgtaaaaga tatgacatac caacaaataa
       61 agcgccgaag ctgttgttga aaggtacagg gaatttaaaa ggttcatcag ttggatataa
      121 aaaaattgaa tttacttttt tagagaataa aaatgaaaat atttacttta ctgatagtct
      181 acatcttgaa ccgagcgagg ataaataatc atcttttaac cagaatatga aatcaatcta
      241 ggacaaccta ataaaaggag agagttgtaa gatgatgaaa cgattaaaca aattagtgtt
      301 aggcattatt tttctgtttt tagtcattag tatcactgct ggttgtggca taggtaaaga
      361 agcgaaaata aagaaaagtt ttgagaagac attgagtatg tatccgatta aaaatctaga
      421 ggatttatac gataaagaag gttatcgtga tgacgaattt gataaaaatg ataaaggtac
      481 atggattatt ggttctgaaa tggcaactca aaataagggg gaagctctga aagttaaagg
      541 tatggtcttg tatatgaata gaaataccaa aacaacaaaa ggatattatt atgtaaatgc
      601 aataaagaat gataaagacg gaagacccca agagaatgaa aaaaggtacc cagttaaaat
      661 ggtcgataat aaaatcattc caacaaaaga aattaaagat aaaaacataa aaaaagaaat
      721 cgaaaacttt aagttctttg ttcaatatgg aaactttaaa gatttgtcga agtataaaga
      781 cggagatatt tcatataatc cagaggtgcc gagttattcg gctaaatatc aattaactaa
      841 tgatgattac aatgtaaagc aattacgtaa aagatatgac ataccaacaa ataaagcacc
      901 gaagttgttg ttgaagggta cagggaattt aaaaggttca tcagttggat ataaagacat
      961 tgaatttacg tttgtagaga aaaaagaaga gaacatttac tttagtgatg ggttaatttt
     1021 taaaccaagt gaggataaat aatcatgact cgatcaaaat atgaaatcaa tccaggaaag
     1081 ataataagaa gaataggatt gtgaaattat gaaacgatta aacaaattgg tattgtatat
     1141 tagttttttg atattagtca ttagtttcac tgctggttgt ggcataggta aagaagcgga
     1201 agttaagaaa agctttgaaa aaacattgag tatgtatccg attaaaaatc tagaggattt
     1261 atacgataaa gaaggttatc gtgatgacga atttgataaa aatgataaag gtacatggat
     1321 tattggttct gaaatggttg ttcaacctaa aggggaacgt atgaaatcaa aaggtatggt
     1381 tctatatatg aatagaaaca ctaagactac aaccgggaag tatatagtga gtgaaacact
     1441 tcatgatgaa gatggtagac ctaagagtaa agataaagaa tatcccgtta aaatggtaga
     1501 caataaaatc attccaacta aaggtatcaa agacgaaaat ataaaaaaag aaatcgagaa
     1561 ttttaagttc ttcgcgcaat atggcagctt taaagatttg tcgaagtaca aagacggcga
     1621 tatatcatat aatccagagg taccgagtta ttcagcaaaa tatcaattaa ctaatgatga
     1681 ctataatgta aaacaattac gaaaaagata taaaatacca acgaataaag caccaaagtt
     1741 attgttgaaa ggttcgggag acttaaaagg ttcatcagtt ggatataaag acattgaatt
     1801 tacttttgtg gagaaaaaag gtgaaaatac tttttttact gatagtctac atcttgaacc
     1861 gagtgaggat aaataatcat ccacacacac gattcaatat gaaattcaaa tatgttgctg
     1921 taaaataatg taaaataaac gtattcatat tactgaattg agggatagta tgcaacgtga
     1981 ttatttaatt cgagtagaaa ctgagagtat gtcagatttc aaaaggctca atagtttaat
     2041 gattggtttt gttattaaag gtgaggcaca catttatgat gaaaataata tgacgcaatg
     2101 caacagtggc gacattttca tcattaacca ccgcgacttg tatcgatttc aacttcaaca
     2161 agatggcatc atatgttata tccaattcca aatgaaatat ttagcagaca agtttgatga
     2221 tgtgcattgt ctatattttc acttaacaga tgcgaccaca acaaagaata tacatcaact
     2281 gagaaatata atggcaagac tggtttcaac acatatccga cataatgagt tgtctaaatt
     2341 gactgagcaa caacttgtga ttcagttgct tatgcatatg attcattatg tcccgcgtac
     2401 atatcattcg aaccaaagta tcttaaatga tgataaagtg aatcaagtat gcgactatat
     2461 cgagttacat tttcatgaag atttaagcct ttcagaatta agcgaatacg ttgggtggtc
     2521 agagagccat ctgtctaaaa agtttacaga atcgctaggt gtaggattcc atcatttctt
     2581 aaatacgacg cgaattgagc atgcgaaact cgatttgaca tacacagatg aaaccattac
     2641 tgatattgca ttgcaaaatg gcttttcaag tgcagcgagc tttgcgagaa catttaaaca
     2701 ctttacgcat caaacaccta aacaatatcg aggtgatcgt ccagcaatca ctgaaaacca
     2761 gcaatcggca caacatgatt atcacgaccg tgaattgata ttacttttaa atgactacat
     2821 tgaagaaatg aatcatttta ttgaagatat tgaaaagatg aactataaag agattgcctt
     2881 tcaaccaacc aatcaccaac taaatcaatt taatcatatt attcaagtgg gctatttgag
     2941 gaatttgctc aatacacagt atcaatcaca gttgcttaca tgtcatcatg attttcaagt
     3001 caatgaagta ttagcatatg atgtgattcc atatatcatg aaaaagctca atgcgccatt
     3061 cacgtatgat gcagaaattt cgaatatatt ttatgatatt gatttgtgtt tagacttttt
     3121 attagatcat aactttagtt tgaccatgca tttggatcag tatgactcac gtgattatat
     3181 cgatgcattt aaaattttta tccatcacgt tgccctgcat gtcagtcata gaaaagattt
     3241 gaagttcaac ttgtatgtga cgacattgca cacgtctttg attgaaatga ttgattattt
     3301 taaagcatta ttccctaatg gtggcttgta cattcactta gatcaagcta cggaaagaca
     3361 tctaccatta ttgaaacgac ttgagccaca catcgaccat tttgtatttg atgccaattc
     3421 aaatgacgct gttgatttta ataaaatgaa tgatgatgaa tttaaaaccg cgagtcaaat
     3481 gattattaat aaaacgaatt accttatcga cttaatgcat cgtcatcgcc taaagcgtcc
     3541 actcatttta ctcaattgga atacattgac gggtgataca tttattacaa acggcgaata
     3601 ttttagaggt ggtatcatca ttgagcaatt attaaaatta agttctaaag tagagggtat
     3661 cggttattgg ttgaattatg atttgcacgt cagtcattgt aaaaatgaac gggattatat
     3721 gaattctatt gaactatttc atcaatataa tggaaaacgt ccggtctatt tcacggcatt
     3781 gctatttaat aaattaacaa gcaatatttt gtattctgat gatacatgta ttgtcacggg
     3841 gactgattca aattttcaaa tattgttata tgatgcaaag cattttaatc cgtacttagc
     3901 gttggacaat caaatgaata tgcgtgcgac ggaaatgatt catttgaaca ttaatgccct
     3961 ggaagaaggt atgtataaga ttaaacattt taccttagat aaagaaaatg gtgcgttatt
     4021 taatctttgg cgcaaacatc atacgataca tggcatggac aaggactcta tagattacgt
     4081 taatcgaatg agttttccga aattagaagt atatgatata gatatcacgg acacactggc
     4141 attaaacatt aaaatgatta cgaatgggat tcacttaatt gaagtaaaac gttacccaag
     4201 ttcataaaat gatcacaaat cacaaatttt gatatacata atttgtgatt ttttatattc
     4261 aaagctaaaa ttgcaaaaaa ttaatggtta acatctctgt tgttggcaat ataaataatg
     4321 attaatcatt tatgatgtaa ctaaggagat gaaggatatg aatcaacaat taattgaaac
     4381 tttaaaatct aaagaaggca aaatgattga gatcagacgt tatttacatc agcatccaga
     4441 attatctttt catgaagatg aaacggcgaa atacatcgct gaattttaca aaggtaaaga
     4501 tgtggaagta gaaacgaatg tcggaccacg tggaattaaa gtaacgattg attcagggaa
     4561 acctggtaaa acattagcaa tccgtgcaga ctttgacgca ttacccatta ctgaagatac
     4621 aggattatct tttgcatcac aaaataaagg tgttatgcac gcatgtggtc acgatgcaca
     4681 tacagcatac atgcttgtat tagcagagac gcttgctgaa atgaaagata gttttacagg
     4741 aaaagtcgtt gtgatacatc aaccagctga agaagtacca ccaggtggtg ctaaaacaat
     4801 gattgaaaat ggtgtattag acggtgttga tcatgtatta ggtgtacacg tcatgagcac
     4861 aatgaaaaca ggtaaagtgt attacagacc tggttatgtt caaacaggac gcgcattctt
     4921 caaattgaaa gttcaaggta aaggtggtca tggttcatca ccacatatgg ccaatgatgc
     4981 cattgttgca ggtagctact tcgtcacagc gttacaaaca gttgtatcta gacgactaag
     5041 tccatttgaa accggtgttg tcacaatcgg ttcatttgac ggtaaaggtc aattcaatgt
     5101 cattaaagat gttgttgaaa ttgaaggtga tgtacgtgga ttaacagatg ctacaaaagc
     5161 aacaattgaa aaagaaatta aacgtttatc aaaaggatta gaggatatgt atggtgtaac
     5221 ttgcacctta gaatataacg atgattatcc agcattatat aatgatccag agtttactga
     5281 gtacgtggct aagacgttga aagaagcaaa ccttgatttt ggtgttgaaa tgtgtgaacc
     5341 acaaccacct tcagaagact ttgcatatta tgctaaagaa cgcccaagtg cctttattta
     5401 tacaggtgca gcggtggaaa atggtgaaat ttacccacat catcatccta aatttaacat
     5461 ttcagaaaaa tcattactta tttctgcaga agcagtaggg acagttgttt tagattacct
     5521 taaaggagat aactaacatg aatgaaacgt atcgcggggg caacaagtta atcttaggta
     5581 ttgtattagg tgttattaca ttttggttgt ttgcacaatc acttgtaaat gttgtaccga
     5641 atttacaaca aagttttggt acagacatgg ggacaattag tattgcggtc agtctaactg
     5701 cactattttc aggcatgttt gttgttggag caggtggtct ggcagataaa attgggcgcg
     5761 tgaaaatgac gaatatcggt ttattattaa gtattattgg ttcagcatta attattatta
     5821 cgaatttacc ggcattatta attttaggtc gtgttataca aggtgtatca gcagcgtgta
     5881 ttatgccttc tacattggcc attatgaaaa cttattatca gggtgctgaa cgtcagcgtg
     5941 ccttaagtta ttggtctatc ggttcttggg gtggcagtgg tatctgttca ctcttcggtg
     6001 gtgcagttgc gacaactatg ggttggagat ggattttcat cttctcaatt atcgttgccg
     6061 tactttcaat gttactcatc aaagggacgc ctgaaacgaa atcagaaatt accaatacac
     6121 ataaatttga cgttgcaggg ctaattgttc tagtagttat gttgctaagt ttaaacgttg
     6181 tcattactaa aggtgcagca cttggttaca catcattatg gttctttggt ttgattgcaa
     6241 tcgtaattgt agcattcttt attttcttaa atgttgagaa aaaagtagat aatccactta
     6301 ttgattttaa attatttgaa aataaaccat atacaggtgc aacgatttcg aacttcttat
     6361 taaacggtgt tgcaggtaca ttaattgtag cgaatacatt cgtgcaacaa ggtttaggtt
     6421 atacagcatt gcaggcagga tacttatcaa ttacttattt aatcatggtg ttattgatga
     6481 ttcgagttgg tgaaaaatta ttacaaaaaa tgggttctaa gcgaccaatg ttattaggta
     6541 cattcattgt ggtcattggt attgcactta tttcattagt attcttacca ggcatatttt
     6601 atgttatcag ttgtgtcgta ggatatttat gtttcggact aggcttaggt atttatgcaa
     6661 caccttctac agatacagct atttcgaatg caccgttaga taaagttggc gttgcttcag
     6721 gtatttataa aatggcttca tcacttggtg gcgcattcgg tgtcgcaatt agtggtgctg
     6781 tatatgctgg tgcagttgct gcaacgagca ttcatacagg tgcgatgatt gcactttggg
     6841 ttaacgtatt aatgggaatc atggcattta tcgcaatttt attcgcgatt cctaatgatg
     6901 ataaacgtgt caaagatgcg aaataataac gatgacacgc caagtgtttt gataatgata
     6961 taaatgaaag taaagcaaat taaatgagga aatgtataga gcagtgcata tagataaatc
     7021 tatgattgga ttaacactaa tataaatata aatgaaaatg aaaaaccatc cacttaaatt
     7081 tttgataagt taggtggatg gttatttatt gcgttgttct aaaaatttgg acatttatat
     7141 ggaaagaact atatataatg tgaaggttat ggcaattcgc ttcaatgaag gcgacgtgta
     7201 aaagtgtata ttaaacgttt cggggtcaga ctccgagaac agtgtccgtc atgcttaccg
     7261 catactaatg aagcattatt atatcagtgt ttatagccga tttaagataa taaattttgc
     7321 taaacaaaaa agccaaccca tgaatgttgg attggctttt tacatgcatc tgaatctcta
     7381 attttaaaaa aatatgaata taaataagac agtaaaaatt aaatttcagt tgttgcaatt
     7441 tcttcatctg taggtacatc atcgttaagg ccaacaagtg cttcagaaac atttcgtgaa
     7501 tgataaccga tacgttcaag aacaccaatc atatcgatat atagtaatcc gccttttgtt
     7561 gtacattcac cacgattaag gcgtttaata tgacctttgc gtagtttatg ttcaatatta
     7621 aatgattctc tactacgttc tacaatttca tcttttttcg ttttgtcata aacatctaac
     7681 atgtcgatgg ccttatcaaa tgactcagca acatggttga ataatttatc cataccgcgt
     7741 tgtgcatctt ctgtaatgcg aatatcttca tcatgttggc gttttaattg agcgacatac
     7801 tcttctgtta gctctgctac ttttaaaata gagcgattga catcaaacat aactgctaaa
     7861 cgctcaacgt ctgccttcgt aatggctttt gtagaaattc taactaaata atttcgaatg
     7921 ctatcattga ttgtttcaac agcttgatgc ttttgttcaa gctttttgat caatttttta
     7981 tcgtcttttg taatttcgcg aatgtcttca aacattgata agacaatctg acccacattt
     8041 tgtaattctt tttgagtttc ttgtaatgca acaccaggtg cgtgataaac aagatctttg
     8101 tttaagtgct gaggtttata gtcatcagca atatctttac ctgggacaag ctttgtaaca
     8161 atccatgcta aacctgctac aaatggtaat tgaatcaaag tatttgttat gttgaagata
     8221 ccatgtgata ctgcaatcgt catcgctggt tttaagtgcc ataaatcttg taacaaacta
     8281 atcaaatgaa tcacaactgg caagaaaatt gtgaagataa ttaccccgat taagttaaag
     8341 atgacgtgta caagcgccgc acgttttgca gcgattgagc cggctaaact agctaagata
     8401 gctgtaatcg tggtaccaat gttatcgcct agtaacacag ggattgctgc gtttaagcta
     8461 attaaatctt gttgataaaa ttcttgtaaa ataccaatcg tcgcacttga actttgaact
     8521 agtgctgtta accctgcgcc gacaatgaca gcgagtattg gatttgtaga catatcaagc
     8581 attaattgct taaatccatc taatgatgct aaaggtttaa cggcatcacc cataaattct
     8641 agaccgaaga atagtgaacc gaaaccgaat agtatgcggc caatgttatt gattttagag
     8701 cgtttaaaga aaaagattaa gaatgcacct aatgctaaaa ttggcattgc atattcgcct
     8761 aaatctatac cgataataaa tgcagttacc gttgttccga tattagcacc cattatcact
     8821 ccaatggctt gtttcaatgt cataaatcca gctgttacca gtccgattgt gataactgtc
     8881 gtacctgaac tactttgtat taaaatagtt acaacgatac ctgcaataac acctaatact
     8941 ggatttgatg taaatttgtt taaaatatct cgtagcctgt ctcctgctga tgcttgaagc
     9001 ccgtctccca tgatttttaa gccgtaaagg aaaataccta aaccacctaa aaaggagaaa
     9061 atgacttctg taaccgacat ttccattatt tcacctcaaa taagctttat atttagatta
     9121 tcgcttataa ttgtaaattt aatgttaaga ttaggtaaaa ttatttaaca atatatgtta
     9181 tttgtatatg acttgtaaaa tatcgtcact tattatgtaa attttcagtg tgaaatggca
     9241 ggtttgcaat cacttgttta acaaaatgat gcaatcaatc atgtaattat gtttcatcaa
     9301 aaaaatcatg agagtggaac aacgaaataa tttttatgaa aacatcattt ctgtcccagt
     9361 ctcatgattt gaaatcacca aataaaaatc tattaatggt tttcgttata acaatttgtg
     9421 ttcttttaat aatgactcaa tgtacgtacc ttttatcttt ttaaggaatc ctgctaatgc
     9481 gagtttttgc attttcgaat ctttagtaat ctcacgcaaa tcttggtggt cattcagttc
     9541 gtatatggca tccattaaga cgcgaagatc aaatggacta ttgatgactt ctggaatacc
     9601 acgatctata tttagtaatt gataaacagc ttccatggca gtacgaaccg aatattctgt
     9661 tgtaaataca gtgtctcgct ctgtttctgc aaagttacca ataaatgcta agttctgaga
     9721 ttgatgcggg acgactaaag gtctgtcgcc gatagcacgc gtcatgaaat aagatgtgat
     9781 atatggcata taaacaggaa tcgtattaga tgcatgtttt gctaagtctt caattttgtc
     9841 agttgataca cctaagtgat acagccattc ttggcatatt tcattaccac tacattctgt
     9901 aattggcttt ttaatataat cgccgtttac atctgaatat aaggcataaa tccatgtaga
     9961 tatttcattt tcaggttggt ctttaaactg ttgctgacga ttgattgtaa aactcatttg
    10021 ccatgcagaa tcattgattg taataatacc gcctgtaact gttttgcctg caagtgggtc
    10081 acgtttacaa atactttcta ttgtatcgat aatctcttta ttgtttgttg tagaagttgc
    10141 tgaaacaaac caactttttt taggaatatt ttggcaaaac ttatcaggat taccaaattc
    10201 aggactttgt cgcgctaaat ttttccatag tgtccaacta ccacctaatt cgtcagttgg
    10261 tggcgctggt gtatcattat caccataagt agagctttct gtaatactac cgtttgtcac
    10321 aaagacaaga tcgtttatag tcagtttaat agattctgca ttaccattac ggtcaattaa
    10381 tatttctcgg gcaatttttt gacttgtcgt aacatctatt ttaatatctt cgacttttac
    10441 atcgtattca aattgaaccc catgcgattt taaatattca accataggta atactaaaga
    10501 ttcatattga ttatatttag tgaattttaa agctgaaaag tctgcgagac cactaatatg
    10561 atgaacgaat cgcattagat agcgacgcat ttccattgca gaatgccacg gttcaaatgc
    10621 aaacatcgtt ttccagtaaa tccaaaagtt tgaattaaag aagtcatcgg aaaatacatc
    10681 tgttattttg acatcatcta aatcttcttc attcgttaag cataaatcta aaatttcttt
    10741 aatcgccgtt ttagtcaaag tgaagtctcc gtctgtgact aaacgttgac cctgtttctc
    10801 aataacacga cagcgagaat agttagggtc ttctttgttt agccaataga actcatctaa
    10861 tacagacgcg ttatcgattt ctaatgaagg gatagatctg aataagtccc acaaacattc
    10921 aaagtggttc tccatttcac gaccaccgcg gacaacatag ccttttaaag gcatattttc
    10981 accatcaaga ctaccacctg ctttaggtaa ctcttctaaa atatgaatct tcgaaccttc
    11041 catttgacca tcccttatta aaaaacaagc tgcagcaagt gaagctagac cagatccgat
    11101 taagtaagcg gatttgtttt ctacattttc aggtttttta gggcgcgcaa atgcttcata
    11161 atttccataa ctgtaataca tatccaccaa gtcctttcgt attagaatac actcagacta
    11221 tacccctttg tgaattgcaa atattagtat ttatattggt aaatttgacg aaattagatc
    11281 atttttatat ttttgatttt taaaatttta tcactcgaat gtttttggat tctgaggtgc
    11341 gggacttgga ataattatgt attttgaaag aagttactta aactaacgcc tagtagagtg
    11401 ataattccgc ctataatagc aagggttgtt ggtagctcgt ctaataacag ataagataat
    11461 aataaagaaa cgataggtgt taaataaaga gacattgttg catcagagac accaactgac
    11521 ttcacaatat aagcaagcaa aacgtatgga attatagtag ggaatatagc taaataaagt
    11581 accgatacta ttgatgtaaa agtggcgccg tgtatatcgt tgatgatttc aggaataaaa
    11641 ataagcataa atggtgagct tgccattatt gtatatagtg tgaaagcgat gaagccgtat
    11701 ttttctatgt attttttctg gaaagtaaaa tacaaacttt cactaaaaga tgcaagtaaa
    11761 ataataaaaa cacctaatac attaatagtt gtgtaatcat ctttacttat tgaaataatg
    11821 gatattccta taaatgcgac gagtgaactt aaccaattcc attttgaaaa atgctctttt
    11881 aaaaatatat aagctaaagc actagaaaaa ataggcgttg tagagactag aattccagat
    11941 atacctgcac taatcaaagc ttcaccaaaa tttaaagctg tgtgatatat cacaaatcca
    12001 caaaatccta aaataaaaat aacagggata tctcttagtt caggggtagg caatttcttt
    12061 ataattacga acggcaagag aattattgtt gctaaaatta aacgaaatgc cgacaatgat
    12121 tctgcactaa aatcatttaa cgcaatcttt atcattggaa atgcagatcc ccacaatatg
    12181 atagtaaata aatatgatag aaaagtagtg tcttgaagtt tattcattaa tatcatcact
    12241 cctttaatta tgtgtttcta tattaaaaaa tatgatttaa aatgagtaca accaattttg
    12301 aatggattta cctatccaat tttaaaaggg agggagaaga tggctaaata taaagatatt
    12361 gctagtgaca taagagataa aataatcaca ggggattggt tttatggaat gaaaatacct
    12421 tcacataggc agttggcgat acagtacaac gtaaatagag taacgattat taaaagtatt
    12481 gagttattag aagctgaagg atttatctat actaaagtag gtagtggaac atatgttaat
    12541 gactatttga atgaagcaca tattacaaat aagtggtctg aaatgatgtt atggtcctct
    12601 caacaaagaa gtcagtatac ggtgcaatta attaataaaa ttgagacaga tgattcgtat
    12661 atacatataa gtaaaggtga attgggtata tcgttaatgc cacatattca attgaaaaaa
    12721 gccatgtcta atacagccag tcatattgaa gacttatctt ttggttataa taatggctat
    12781 ggttatatca agttaagaga tattatcgtt gaacgaatgt caaagcaagg tataaatgta
    12841 ggtagagaaa atgtaatgat cacttcaggc gctttacatg ccattcaact tttatctatt
    12901 gggtttttag gtcaagatgc cataataatt tcgaatacac catcatatat tcactctaca
    12961 aatgtttttg agcaattgaa ttttagacat attgatgttc cttataatca aattaatgaa
    13021 attgatacca tcattgatag atttattaat tttaaaaata aagcgattta tatagaacct
    13081 aggtttaata acccgacagg tcgttcttta acgaatgagc aaaagaaaaa tataattact
    13141 tatagcgaaa gacataatat tcctatcatt gaagatgata tctttagaga tattttcttt
    13201 agcgatccaa ctccttctat caaaacttat gataaattgg gaaaagttat acatataagc
    13261 agtttttcaa aaacgattgc accagcaata agaataggtt ggattgttgc ttctgaaaaa
    13321 ataatagagc aattggcaga tgtaagaatg caaattgact atggatccag tattttgtca
    13381 caaatggttg tatatgagat gttgaaaaat aagtcttatg ataaacactt agtaaagtta
    13441 aggtatgttt taaaagataa acgagacttt atgttaaaca tcctcaataa tttatttaag
    13501 gatatagcac attgggaggt tccaagtgga gg
//
LOCUS       DAMQYT010000057        13239 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMQYT010000057 DAMQYT010000000
VERSION     DAMQYT010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13239
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.057"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            111..1649
                     /locus_tag="PGA33_001241"
     CDS             111..1649
                     /locus_tag="PGA33_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDG6473062.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            1684..3816
                     /locus_tag="PGA33_001242"
     CDS             1684..3816
                     /locus_tag="PGA33_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDG6473063.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLDQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDVDGNTSFENEQTSYLTSVFEKHLSDEKSEQLLHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFISDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            3828..4262
                     /locus_tag="PGA33_001243"
     CDS             3828..4262
                     /locus_tag="PGA33_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDG6473064.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            4262..5368
                     /locus_tag="PGA33_001244"
     CDS             4262..5368
                     /locus_tag="PGA33_001244"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDG6473065.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVEDSSIEAGLRRVLAETSQYITNEFDFTEAEQAAYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(5687..13123)
                     /locus_tag="PGA33_001245"
                     /pseudo
     CDS             complement(5687..13123)
                     /locus_tag="PGA33_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
BASE COUNT         3604 a         2211 c         2359 g         5065 t
ORIGIN      
        1 ttaattttgt cacatttatt ttgacaaagt tgatttttgt ttatattgag taacaagtaa
       61 cctctctata cactatatat agtcacatat attaaaaaag aggtgtaaac atgtcacaaa
      121 ctgaagagaa aaaaggaatt ggtcgtcgtg ttcaagcatt tggatcattc ttaagtagta
      181 tgatcatgcc aaacattggc gctttcatcg cttggggttt tatcgcggcc atttttatag
      241 ataatggatg gttacctaac aaagatttag ctactttagc aggaccaatg attacttatt
      301 taatcccatt attgatcgca tttagtggtg gtcgtttaat ttatgattta cgtggtggta
      361 tcatcgcagc aacagcaact atgggggtca tcgttgcatt acctgataca ccaatgttac
      421 ttggtgcaat gattatgggt ccacttgttg gttggttaat gaagaagacc gaccaattga
      481 ttcaacctag aacgccgcaa ggttttgaaa tgttatttaa taacttttct gctggtattt
      541 taggatttat catgacgatt gcaggattta aaattttagc accacttatg aaatttatta
      601 tgcatatttt atcagtggca gttgaagcat tagtacatgc acacctactt ccacttgtaa
      661 gtattttagt agagcctgca aaaattgtat ttttaaacaa tgcgattaac catggtgtat
      721 tcacaccgct tggtgcagat caagctgcaa aagctggtca atcaatttta tacacaattg
      781 aatctaaccc tggaccaggt ttaggtatct tacttgctta tatgattttt ggaaaaggca
      841 ctgcaaaagc aacgtcatat ggtgctggaa ttatccactt cttaggtggt attcatgaaa
      901 tttatttccc atatgtatta atgcgtcctt tattatttat cgctgttatt ttaggtggaa
      961 tgactggtgt tgcaacttat caagcaacag gatttggatt taaaagtccc gcgtcaccag
     1021 gttcatttat agtttactgt ttaaatgcac ctagaggtga attcttgcac atgttactcg
     1081 gtgtcttcct tgctgcactt gtatcattcg ttgtagctgc tttaattatg aagttcacta
     1141 gagaaccaaa gcaggattta gaagctgcga cagctcaaat ggaaaatact aaagggaaaa
     1201 aatcaagcgt tgcttctaag ttagtatctt ctgataaaaa tgttaataca gaagaaaatg
     1261 ctagtggtaa tgttagtgaa acatcttcat cagatgatga tcctgaagcg ctattggata
     1321 attacaacac tgaagatgtt gatgcacaca attacaataa tataaatcat gttatttttg
     1381 cctgcgatgc gggtatgggt tctagtgcaa tgggtgcaag catgttacgt aataaattta
     1441 aaaaggcggg cattaatgat attacagtta caaatactgc gattaatcaa ttgccaaaag
     1501 atgctcaatt agttatcact cagaaaaaac taactgatcg tgctattaaa caaacaccaa
     1561 atgccatcca tatttcagtg gataatttcc ttaattcacc aagatatgaa gaacttttaa
     1621 ataatctaaa aaaagatgat caagcataat aattaaataa attaaaaaat ggaggatacc
     1681 gccatgttat tgagtacacg tgaaaaagaa atgatagccc tattgattaa gtaccacggt
     1741 caatatatca ctatacacga cattgctcag caacttgcgg tgtcctctcg tactattcac
     1801 cgtgaattaa aaggtgttga agcatattta acttcatttt cattaacttt agaacgcgca
     1861 aacaaaaaag ggctacgcat tgctggcaca gattctgatt taaacgattt gaagcaatcg
     1921 attgcacaac atcaaaccat tgacttatct gttgaagagc agaaagtaat tattatatac
     1981 gctttgatac aagccaagga gccagttaaa caatatagtt tagcgcaaga aattggcgtt
     2041 tctgtccaga ctttagcaaa gatgttagat gatttagagc ttgatttaaa taagtaccaa
     2101 ctatctttat ctcgaaagcg tggcgaaggc atttacttgg taggtactga atcaaagaaa
     2161 cgtgaatttt taagtcaatt aatggtgaat aacttaaata gtactagcgt ttattcagta
     2221 attgaaaatc attttgtctt tcattcatta aatcaaatcc acaaagactt tgttgactta
     2281 gagcgcattt ttaatgttga aagactatta atggactacc taagtgcctt accctaccaa
     2341 cttaccgaat caagttattt aactttaact gtccatatcg tgctctccat ttcacgtata
     2401 aaaaatggag agtatgtcgc attaaacgat gatatttatg attctgtaca aaacacattt
     2461 gaacacaaag tagcaagcga acttgctgat aaacttggtc aaatatatga cgtcacgttt
     2521 aatcaggcag aaattgcttt cattactatc catttacgtg gagctaaacg aaaaaatctt
     2581 aatgatacat cattaaataa tcgttgtgaa gaaaacaaaa ttaaagcgtt tgttaacaaa
     2641 gtagaaatga tttccggtat gacatttgca gatttggata ctttagtaga tggactgacg
     2701 ctacacctta atcctgcaat caatcgtttg caagctaata tcgagaccta taatccgtta
     2761 acagacatga ttaagttcaa atatccaaga ctatttgaaa atgtaagatt agctttaaat
     2821 gattgttggc ctgatttgat ttttccagag agtgaaattg cttttatagt tttacacttt
     2881 ggtggctcga ttaaaaacca aggtaatcga tttttaaaca tattagtcgt ttgcagcagt
     2941 ggtatgggaa ctagtcgtct attatcaact cgtctagatc aagtttttag tgagattgag
     3001 cgtattacac aagcatcagt cagcgatttg aagtcactag atttaagtca atatgatggc
     3061 attatttcta ctgtgaattt agacatcgac tccccctatt taacggtaaa cccattatta
     3121 ccagatagtg atatcagtta tgtcgcacag tttttaaata caaagtctac gttccaagag
     3181 acgcatgata aatcatcaaa catgattgat aaggatgatg ttcatgttga aacgaaagat
     3241 gttgatggca acacatcttt tgaaaatgaa caaacttcat acttaacttc agttttcgaa
     3301 aaacatttaa gtgacgaaaa atcagaacaa ttattgcatc atatgcgttc gggtttaact
     3361 ttgcttgatt cagtgaaaat agttagtacc gaagttaaac agtggcaaac atatatcgca
     3421 gattatctat atcaatgcga tgtaataaac gatccaacgt cattcgctga actactagag
     3481 caacgattga ttgacaatcc aggatggata ttaagtccat atcctgttgc aataccacac
     3541 ctaagagaca atatgattaa acaccctatg attctaatca cagttttaga agaaccgtta
     3601 acattgccta gtattcaaaa tgacaatcaa acaattaaat atatgatttc catgtttatt
     3661 tctgacaatg attttatggc atcactggta agtgacttgt ccgaattttt aagtttgaaa
     3721 ttagaatcta ttgatacttt tatggaaaat ccacaggaac ttgaaacatt attaagaaac
     3781 aaatttttag aacgaattaa aaaacaattt atttaggagt gtacaaaatg agcgaattat
     3841 ttagtaatga caatatcttt ttaaatgtaa atgttaacag ccaaaatgaa gcaattgaaa
     3901 aagcaggtaa agccttagtt gatagtggtg ctgtaacaga tgcttatatt caagcaatga
     3961 aagatcgtga gcaagtcgta tcaacattta tgggaaatgg cttagcaatt cctcatggca
     4021 cagatgaagc taaaacaaat gtgattcact caggtttaac attattacaa atccctgaag
     4081 gcgttgactg ggatggcgaa gtagttaaag ttgtcgtggg aattgctggt aaagatggcg
     4141 aacatttaga cttgttatct aaaattgcaa ttacatttag cgaagaagaa aatgtggatc
     4201 gtatcgttca agcaaaatct gcagaagaaa ttaaacaagt attcgaggag gcagatgcat
     4261 aatgaaagca gttcactttg gtgctggtaa cataggtcgt ggtttcattg gttatattct
     4321 tgcagacaac aatgttaaag taacatttgc agacgtcaat gaagaaatca ttaatgcttt
     4381 agctcatgat catcaatacg atgttatttt agctgatgag tctaaaacaa cgacgcgcgt
     4441 gaataatgtt gatgcaatta attcaatgca accttctgaa gcgttgaaac aagcaattct
     4501 agaagctgat attattacaa cagctgttgg tgttaacata ctacctatta ttgctaaatc
     4561 ttttgcgcct ttcttaaaag aaaaaacaaa ccatgttaat attgttgctt gtgagaatgc
     4621 tattatggca actgatacat tgaaaaaagc agtacttgat attactggcc ctcttggtaa
     4681 caatattcat tttgctaact cagcagttga tagaattgta ccattacaaa agaatgaaaa
     4741 tatattagac gttatggttg agccatttta cgaatgggtt gttgaaaaag atgcatggta
     4801 tggtccagaa ctaaaccata ttaaatatgt tgatgattta acaccatata ttgagcgtaa
     4861 attattaact gtgaatacag gacatgcata tttagcgtat gctggtaaat ttgcaggtaa
     4921 agctacagtt ttagatgcag ttgaagatag ttcaattgaa gctggcttac gccgtgtttt
     4981 agctgaaact agtcaatata ttactaatga atttgatttt actgaagcgg aacaagctgc
     5041 ttatgttgaa aaaataatag atcgtttcaa caattcttat ttatctgatg aagtaacacg
     5101 tgtcggacga ggtacattac gtaaaattgg ccctaaagat agaattataa aaccattaac
     5161 atatctttat aataaagatt tagaacgcac tggtttatta aatacagctg cattgttatt
     5221 gaagtatgat gatacagcag accaagaaac tgttgagaaa aataattaca ttaaagaaca
     5281 cggtttaaaa gcgtttttaa gtgaatatgc taaagttgac gatggcttag ccgatgaaat
     5341 aattgaagcg tacaattcac tttcataatt tattgagctt tgtttgaaac aagaagtttc
     5401 caacgttatt cgttaacaat cagtaataat gtagtagttc ccttgaatta acaatattaa
     5461 atttctgaac ataaaaaata ctcccttcaa catagacact taacttgtgt tatgtatgaa
     5521 aggagtattt ttgcgttaat aatttgtttt attttcgagc cacagccacc tattcaatgg
     5581 ctattggtca ttactaaaac aaattcatat taactgttag acttggttac ttagtaagga
     5641 atatttccct atgaaataac tagatgttca cattcttgaa taaattttat tcttcagttt
     5701 gttggtcttt cttagtgaat cttctaatta agaatgccat acctgcacct agagctaatt
     5761 cagcatatgg taaatcgtca ttatgtgaca taccagtatc tggtaaagtt ttagcttgtt
     5821 gtttagcttt attaactttt ccttgttgag ctgattttgt cttagcttgg tggtcgtcag
     5881 tgttagttac attaagcata tcttgattag cactattgct tccatttgaa actgtagctg
     5941 gagatgcatt ggcaccgtcg ttttgcgtag ctttattgtt tgcagctgaa ccaactgatt
     6001 tttgcgtatc attagtatct gctgttgccg tatcatcttt ttggctaaca ttagttgaag
     6061 tcattttttc ttttgcttca gaagatgcag atgttgatgg tttattcgaa acttcagtat
     6121 cagctttgct tggcgattta tctgcttcgt tagatgcaac gttagtttca gacttaagtt
     6181 gtcctgcatc agtttgattt gtcgtacttt cttctttatc ttttgatgta ttagaaggta
     6241 catttggttc tgttatgtct gctgaaggca atgtttcagt tgttgattca accatacttt
     6301 gatttgttga atcactacca tctttttctg ccttagcttt attttcagat tttggttgtg
     6361 caaccttgtc attagttgat tgagattcag cactattatt tacttcagca ttttgttttg
     6421 aatcatttac agatgcatta tctttgctat cagcagatga tgctgcttct gtgctcgcag
     6481 ttgttggagc cgttgctgtt gatcctgttg gtgcattctc gtttgttgct gtagttgtac
     6541 tattgttatt tgttgtgctt tctgctggcg ttgcattatc agtttctgtt acaggtttat
     6601 cagttgtgcc gttattagtt gattctactt ctggtttact agttacatcg ttatccattg
     6661 tcggactgtt tgttgatgca tctacactag aattgttatt agcttgcggt ttatcatttg
     6721 catcatcagt tgctgatgtt gctgttgttt cacctgttgc cgcatcacta ttatttggtg
     6781 ttgtcggaga agcgtctgct ttgccattag ctgtcgtctc agatacgtta ggttgtccag
     6841 tattttctgg tgttgcatta gcatttgaat ttgctgttgc atcattatta tctataccat
     6901 tattagtatc attagcatct ggatcattct gaggcacaat cgcttcaatt gcaggtatcg
     6961 ttacattttg taattcagca acttctgcat ttgtttgtgt tttatctaat ttatcagcaa
     7021 atctgtcaaa atatctacct aaatccgtac gtgcaatttc tttcgccgat gcatctgcat
     7081 ctgcattttt aattatttct atttgcttgt taaccacttc tctgattgct tccaaagcat
     7141 ttttcttaac ttcaggatta atacgttgtg ctttaagttg ttcaagcgca ctatttttga
     7201 cagtagcgat ttctgcattt gtagtttgat cagaaatatc ttcagttgct tttgataaaa
     7261 tgtcttctaa agcattcgta aacgcttctt tttcttcagt tgtagcatca gcgttgacat
     7321 ttacacctgc ttcaatctgg tctagtgcag tttctaattc ttcgatagct ttttgttttt
     7381 ctgttgagtc gatttgaatg ttatcaaatg cttcaagtcc ttgagctttc gctttttcaa
     7441 cttcagcagt tgttgttgca tcagtaatac cttgtttagc ttgatctgta atttgtttaa
     7501 tcattgctaa tgcttcagtt ttttcttcag cagttaactg gtcactttga tcaatagatt
     7561 ctttcgtatc ttctgcttta acttcaatag cttggttcgc tttaggttta acagtagcat
     7621 ctacttgaat agcatcaatt gctgctttac cttgtgttaa tgcatcatca acgtcaccat
     7681 tatccacacc attattaatg ctttctaatg cagtttgaac attttggtca acttgcttaa
     7741 ttgcttgttg cttttcatct tgtgttgcat tagtgtcagc tgaaatatta tttttcttct
     7801 gatctgcata agcatataaa tctgttgtag ctgattcttt tttacctgtt ggaattgtgt
     7861 aatcgttaat attatctaag tcattatgaa tttgaacttc aatgtcatct ttagaagtag
     7921 cttgattaac attttgatcc gcagtttgtt ttaattcagc aagtttttgt ttcgcttcag
     7981 caatttcact tgaagtcgat gcgttagagt tatccgcttc gtttacttta gcattatatg
     8041 catcttcaat tttagctaaa gcatcttttt tgtactcact aaatgtttta actgcattaa
     8101 ttttagcttt tccttcttta actgcattat caacatattc atttgttgat gattgatcga
     8161 catttgtttt cgcttgattt aattcagtat caacttcttg ttttgcatca ttaatttctt
     8221 gttgtgatgc atttggtgtt tgttctattt gtgttttctt atcagctgca gcttgatcta
     8281 attctttttt acctgctggt ttcttaacag gatttgcatg aagttgttga actttttgta
     8341 ctgctgtatc tttagcagta gttacatcac ctgtagtagt tgctgcatta atattattta
     8401 aaccttcttc atatgctgct ctaactggtc caatatcgtt acctttttct tcattagtag
     8461 tctcattatt attaagtatt tcagttattt tattttgcat ttcagttagc aattccgctc
     8521 ttgcattcgt tttaacatct gttgatgctt gaattgggtc aattgcttga attgcattat
     8581 cttttgcagt gtttacatca tcgattgact gtgcattttc aatattttga ttaccttgtg
     8641 ttaattgtgc gtctacttgt tgatttgctt gttctttttc ttcagtagtc gcatctgcag
     8701 tttgtgcaat aagcgctttt tgttcgtttg cttttgttgc taattcatct ttcgcaacat
     8761 ctttaattgt tgtatctgca gtaatacctt gaatatcagc aactgcttga tctttaattt
     8821 gcgtaacatc attagttgtt tgtgcattta agatatcttg atacgctttt tctttagctt
     8881 ttaaaactaa atcttttgct gcatttttct cttcagttgt agcaccagtt gtattatcaa
     8941 ttgcttgatt ttgagttgtc acagcttgat caacatcatt tttagcattt gatttaaccg
     9001 ctgttgctgg ttgcgtgctt tgaattgaat tctttccagc gtctttcgcc tgatctacac
     9061 cattatcatc agttgcagct gtaatattat ttttcgcgtc tgtaactgct gttgctaatt
     9121 gttgaatagc tgcttctttt tcttctgttg tagcgttctg atcattattg ataacatttg
     9181 tttgcgttgc ttgtaattga tcaatttcat ctttagccgc ttgtttcttc acaacttttg
     9241 gtgttaccgc attaatcgct gcttctgcat ttgctttagc ttcatcaact tgtgcgttag
     9301 tagttgctgc tgaaatggct tgatttgctt taccattttc agtatttgct tcagcatcag
     9361 ctgcttgttt ttcttcatct gttgcatctg gcgtagcttg aatctcttgc aatttgttat
     9421 ttaaaattgc tgtgatttca ttacgtgcag ttgctttttt attaactgtt ggtgttactt
     9481 gatcaatact attttcacct gtcgttttcg cttggtctac atcattttga ctattagcag
     9541 cttcaatgtt gctatttgct tgtgtcacag cattatctac gtccgcatta gccgctgcaa
     9601 tttcttcagc agtaatgtct tgcgtttgag cgattgctgt tttacgttca ttcgctttcg
     9661 tagcaattgc ttctttcgca ttatctttag ttgttgttgc tggctgaatc gcttcaattt
     9721 tagcaattgc tgctttttta gccgcttcaa cttccgcatt tgtatgtgct gcatctattg
     9781 cggcatcagc tgttgttttt tcagtttgaa cttgttattt agcagctgct ttttcttcag
     9841 ttgttgagcc gttatttcca tcaattgctg tttcttgagc ttgtacttta tctgcaattg
     9901 cttgttttgc tgctggttta acatttgcat caggtgtaat ggctgcgatt gtagcttcat
     9961 ttgtagtttt tgcattatcc acatcattgt ttgctgcagc attatctata tcagcgtttg
    10021 cagtaactac tgcttgatcc actttgtctt tcgctgcttg ttgttcttca gtagtcgaat
    10081 cattcattgc ttcaattgct gttttacgtt cacttgcttt ttgagcgatt tccgcttttg
    10141 catccgattt cttagttgtg gcagcttgaa cttgattaat tgcagcaata ctattgtctt
    10201 tagctgttgt tacatcactg tttgtatttg cagcatcaag atttgttctt gcttcttgct
    10261 ttttagtatc taattctgta tatgcagctt gtttttcttc tgtagttgaa gcattgctat
    10321 tttgaatttc ttgtttacgt gtattatatg cgttttctac ttccgtatca gctgcaggtt
    10381 taacttttgc ttgtgtttga attgcattga ttttatctaa tacttttgat tttgtatcag
    10441 caacttcctg ttttgattta acaacttgga tgtcatgtaa accttgcttt tgagctgcat
    10501 ctactgctgc atcagcttct gctacttctt catttgttgc atttgaaact gtagcatttt
    10561 gagcttttct agctgttgca gcttgtttaa cttcattata ggcatccatt tttgtttgcg
    10621 tagagtcagt aatattttca atttttgaga tttcttcgtt tttaactctt tctaaatctt
    10681 gtgcagtcgt tgtcgcttca attgctttaa caccagaaac tttagctgca ttaatacgtt
    10741 caatagcttc cgctacttct tcatttgttg tatcaggatt taaaggtgct tgatcaattt
    10801 gtgcttgaac aacttcgtca aattcttcaa gagctgctgc tttagctgtt gtagcaggtg
    10861 tagtttgatt aatatcattg attgcttttg ttttaatggc ttctacttgt gcatttgttg
    10921 ttgctgcatc aatatcttta attgcctttg tttcaatttt accaatttta tcatttgcta
    10981 catctttttc atcttgtaat gatgcatttg actttttaat ttgttgttta cgagttgtaa
    11041 ctgcttggat aatatcttgt ttcgcttgag gtttaactgt tggtgtaatc acatcttgtt
    11101 ctaacactgc gataccatta tctttttcag ttgtgacacc ttgtgctgta gtttgtttat
    11161 taatttcagc gattgcatta tttttatcat tgttaatttt agttacaagt gctgcaactt
    11221 catcttgcgt aactttttta ctttgttgtg ctgctgtaac cttttcttga gctttagtat
    11281 ctaattcagc aattgctgct tgattatcaa ttaatgcagc ttgtaattta gttactaaac
    11341 catcaatatc cgcttgagat gcacgatttg cagttttaac atgattcgca tcttcattta
    11401 aaatagtatc tgcttgttgt ttaagtttat tgtattctgc aattgatgct gttgtgtaat
    11461 gactattatc aacttgtgag tttacttgtt gttgcaacgc atctttgttc atttcaaccg
    11521 ctactgaaaa tggatctgca gttagtgtaa cttctggttg cgcattatta attacaacat
    11581 ctgaagcagt acgatatgtt aatttttcat taaaatcaat atttttaggt gtatcgatat
    11641 tcgcaacatt aactttatat gataatttta aagatttatc tgggaataaa acttctttag
    11701 tgtgtgtacc acgtgccgtt gtcacacctt ggcttgtaaa tgtaacttta tttgcatttt
    11761 gatcataatt aacagtcata tttttcaata ctgtactgtc ttcattacca ttagggaatg
    11821 ttgtagttaa tgaattatta acgtaagtta caccttgtgg taatgttact tcatatttaa
    11881 attgatctgc ttttaatgaa gcaccaaaat taccattgtt accgatttca gtattaatag
    11941 tatattcttt attgttagtt gctttcggtg ctaaatctct atttttgaca tataaatgtg
    12001 atcctgagaa tagacctaca ttatcaatga atgaatagta tttataacca tctttatttg
    12061 ttgtaattct tgaagcatca gcaactgctg tattgtcagg aataaattgt aaatctaaac
    12121 gatcagcatc agttggtact ttaaataaat gtgcagtacc agtcttagca acatttacag
    12181 ttgctaattc agtatttttc ttagcatctt taataactaa atttgttgca ccttgcttac
    12241 tattcgtttg catagtagta aagttaataa ttaattctga atctggtttt acatttacag
    12301 tttttgaaat accgttaaag ttaccatgat ctgtagaatc atttgcattc acacgaccta
    12361 atgcagccac gtttccttta gcttgatagt tttgagggtt attcttatca aacatatcgc
    12421 ttcgtcttaa ttctgagtta acgaaaccaa tcttaccgtt gttaattaat gaataaccat
    12481 ttactttatc tgtaacagtt acagttggat cttgtctatt ctcatctgtt gatatggcag
    12541 gatcatcaaa tgttaatgtc gtattaatac tgccttcacc agtattgcta gcatttggat
    12601 cttgagtttg tgcgtttgct gctacaggtg ctgctggttg cgctgctgct ggagcattcg
    12661 ctggctgtgt ttgatttgcc ggtgttgcat tattattagg tgttgcttgg ttatttcctt
    12721 gacctgcttg gtttgccggt gttgcttgat ttccaggttg tgcttgtgca gcgttattcg
    12781 gatcagcttg attaccttgt ccagctggtt gtgtatttgg ttgtgctgct cctcctgctg
    12841 gattagcctg tccaccttgg tttgctggtt gtactgctgg ttgtccttgg ttggcaggtg
    12901 cagctggctg tgctgtagga ttagcttgag caccagcatt tgcgttaggc tgtgtattgg
    12961 catcagctgg ttgtgctggt tgattttgtg caggctgatt ttgctctgct gcagacgctg
    13021 ttgtcgggtt agtagatata aaagtaacag tggcaattaa agctgaaaaa ataccgacat
    13081 taaattttct gatactaaat ttttgttgtc tgaataaatt cattaagtca tcctcctggt
    13141 tgattattct cgctgttaaa tgatttcact taatcaactg ttaagataag tagtagcatc
    13201 tgcgttaaaa acacaaagca actctatcta attaaaatt
//
LOCUS       DAMQYT010000058        13146 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMQYT010000058 DAMQYT010000000
VERSION     DAMQYT010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.058"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..992
                     /gene="coa"
                     /locus_tag="PGA33_001246"
     CDS             <1..992
                     /gene="coa"
                     /locus_tag="PGA33_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HDG6473066.1"
                     /translation="PNFDKLVEETKKAVKEADDSWKKKTVKKYGETETKSPVVKEEKK
                     VEEPQAPKVDNQQEVKTTAGKAEETTQPVAQPLVKIPQGTITGEIVKGPEYPTMENKT
                     VQGEIVQGPDFLTMEQSGPSLSNNYTNPPLTNPILEGLEGSSSKLEIKPQGTESTLKG
                     TQGESSDIEVKPQATETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEAR
                     PRFNKPSETNAYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHGNGQVSYGARPTQN
                     KPSKTNAYNVTTHANGQVSYGARPTYKKPSKTNAYNVTTHADGTATYGPRVTK"
     gene            complement(1174..1281)
                     /locus_tag="PGA33_001247"
     CDS             complement(1174..1281)
                     /locus_tag="PGA33_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473067.1"
                     /translation="MCVRTRLVSSSSARLSKAIIIAVIVVYHLDVRGLF"
     gene            complement(1595..2779)
                     /locus_tag="PGA33_001248"
     CDS             complement(1595..2779)
                     /locus_tag="PGA33_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDG6473068.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIQVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GFKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            complement(2809..5070)
                     /locus_tag="PGA33_001249"
     CDS             complement(2809..5070)
                     /locus_tag="PGA33_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HDG6473069.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKISIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDTIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPISHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            complement(5256..6467)
                     /locus_tag="PGA33_001250"
     CDS             complement(5256..6467)
                     /locus_tag="PGA33_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDG6473070.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            complement(6579..8084)
                     /locus_tag="PGA33_001251"
     CDS             complement(6579..8084)
                     /locus_tag="PGA33_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HDG6473071.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDNDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILNQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            complement(8110..9687)
                     /locus_tag="PGA33_001252"
     CDS             complement(8110..9687)
                     /locus_tag="PGA33_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDG6473072.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEIYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
     gene            10128..11270
                     /locus_tag="PGA33_001253"
     CDS             10128..11270
                     /locus_tag="PGA33_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDG6473073.1"
                     /translation="MSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMLSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVVLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            complement(11584..13059)
                     /locus_tag="PGA33_001254"
     CDS             complement(11584..13059)
                     /locus_tag="PGA33_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDG6473074.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKMLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
BASE COUNT         4152 a         2449 c         2259 g         4286 t
ORIGIN      
        1 aacctaattt tgataaatta gttgaagaaa cgaaaaaagc agttaaagaa gcagatgatt
       61 cttggaaaaa gaaaactgtc aaaaaatacg gagaaactga aacaaaatcg ccagtagtaa
      121 aagaagagaa gaaagttgaa gaacctcaag cacctaaagt tgataaccaa caagaggtta
      181 aaactacggc tggtaaagct gaagaaacaa cacaaccagt tgcacaacca ttagttaaaa
      241 ttccacaggg cacaattaca ggtgaaattg taaaaggtcc ggaatatcca acgatggaaa
      301 ataaaacggt acaaggtgaa atcgttcaag gtcccgattt tctaacaatg gaacaaagcg
      361 gcccatcatt aagcaataat tatacaaacc caccgttaac gaaccctatt ttagaaggtc
      421 ttgaaggtag ctcatctaaa cttgaaataa aaccacaagg tactgaatca acgttaaaag
      481 gtactcaagg agaatcaagt gatattgaag ttaaacctca agcaactgaa acaacagaag
      541 cttctcaata tggtccgaga ccgcaattta acaaaacacc taaatatgtt aaatatagag
      601 atgctggtac aggtatccgt gaatacaacg atggaacatt tggatatgaa gcgagaccaa
      661 gattcaataa gccatcagaa acaaatgcat ataacgtaac aacacatgca aatggtcaag
      721 tatcatacgg agctcgtccg acacaaaaca agccaagcaa aacaaacgca tataacgtaa
      781 caacacatgg aaacggccaa gtatcatatg gcgctcgccc aacacaaaac aagccaagca
      841 aaacaaatgc atacaacgta acaacacatg caaacggtca agtgtcatac ggagctcgcc
      901 cgacatacaa gaagccaagt aaaacaaatg catacaatgt aacaacacat gcagatggta
      961 ctgcgacata tgggcctaga gtaacaaaat aagtttgtaa ctctatccaa agacatacag
     1021 tcaatacaaa acattacgta tctttacaac agtaatcatg cattctatga tgcttctaac
     1081 tgaattaaag catcgaacaa tcggaagcat atttctaaat tatttattca ttatagtctt
     1141 aaacataaca tgacctaata tattactaac ctattaaaat aaaccacgca catctaagtg
     1201 atatacgaca atcacagcaa taataattgc tttagaaagt cgtgccgaac tggaacttac
     1261 aagtctagtt cgaacacaca ctgatgtgag tggttttctt tattttaaac atgaacaatc
     1321 agataagtta ctagcattag caaatattat taaatcaaag ggcttcgatt cataaaattt
     1381 aaaacaatga ttaaaattag acgtgtaaat gttaaattct aaaacggaaa taaccaccat
     1441 cccattaaac cacttttttg ttcaatcact atatttcaca cagcttcatt aataaaacga
     1501 aattgcttca acccgcttca acttcaactg gcttcaactt cagcctactt cattcaataa
     1561 caaaacgaat ccgcttcatc caaaatcaac cattctaacg cacatattca aatatagcag
     1621 ctgcacccat gccgacacca atacacatcg taaccatgcc gtaacggcta tcgggacgtc
     1681 tacccatttc attaagtaaa cgcgcggtta acattgcgcc tgtagcacct aatggatgac
     1741 ctaaagcaat agcgccacca ttcacattcg tacgtgatat atctagacct acttctttaa
     1801 tagatgcaat cgtttgagaa gcaaatgctt cgttcaattc gatcaaatca atgtcttcaa
     1861 cagatagatt gctgagtgac aatacttcag gaatcgcata tgcaggccca atacccataa
     1921 ttttcgggtc aacgcctact gccttaaaac caacgaatcg tgcaataggt gtcacgccga
     1981 gttctttcac tttatctcca gacattaaaa ctacaaatcc tgcaccatca gaaagtgggg
     2041 cagatgttcc tgcagtcaca gtgccgtcag ctttaaatac tgtacgtaat ttggctaatg
     2101 cctccatcgt ggtgtcaggg cgtataaatt catcttggtc aaagatattt gtgtgtactt
     2161 ttggtcctgc gtttgtatat tcaactgagt ttacttgtat tggaataatt tcatctttga
     2221 accgaccatc acgttgtgcg tcataggcac gttgatgact tctgacagca taagcatctt
     2281 gatcttcacg tgatacgtca aattgggatg ctacattttc agcagttaaa cccataggat
     2341 atgacgcacc tatatcatca tattgtaagg ttggattgtt tgtgggctcg ttgccaccca
     2401 ttggtacggc actcatcaat tcaacgccac cagctacaag tatatctcct tgaccagcca
     2461 taatttgatt ggctgcaatc gcgatggttt gtaatcctga tgagcagtag cgattcactg
     2521 tttgacccgg taccgtgtca gataatcccg cacgcaatgc aatcgttcgt gcaatgtttt
     2581 ggccttgtaa tccttctgga aaagccgtac caacaatgac atcttcaatc atattcttat
     2641 tgaattttcc gtcaatacgt ttcaatacgc cttgtaatac tttggctgcg acatcatcag
     2701 gtctttcgtg gaataatgcg ccttgctttg ctttcgctgc ggctgaacgc ccataagcta
     2761 caatgtatgc ttcttgcatg gttatcatcc tctcttaatg actatctttt aattacgtaa
     2821 tggcttacca gtttttaaca tatgtgcaat tctttcatat gattttttag attttagtaa
     2881 gtcaataaag ccaattttct ccaacgattg aatgtaacgt tgattgataa atgtatttct
     2941 tggtaaatca ccacccgcta aaattgtggc gatatttaag gcaatatgat aatcatggtc
     3001 gctaataaaa tgaccccgtc tttgcgcatc taattgtcct tggatcaatg ctttgaagtc
     3061 ttcacctaaa gcgatatatt gatgtctagg attcggaata tagtttgttt ctgcttcata
     3121 tttcgcacgt ttgagcgcaa cttcgacacg ttgtgctgta ttgaaaataa tcgtatctgt
     3181 atcacgtaaa taaccataac gacgtgcctc aaaggcattt gtagagactt tcgcaaatgc
     3241 gatattcgtc agtacttttg tcatggaagc ttgtttgtca tcaaacttat gcgatgtgcg
     3301 taatatgcga tcagccattt ctgcaaggcc accgccactc ggtaataagc caacacctgc
     3361 ttcaacaaga ccgatatatg tttcacttgc agcgacaaca ataggtgagt aaagtacaag
     3421 ctcacagcca ccgcctaagg cacgaccttg aacagctgtg actactggtt tcaaactata
     3481 cttcaaacga ttaaagctat aatgtaattt atcaattgat tgtgcaacga catcatctac
     3541 aagaccgtct tcatgcgcct ttttcattaa gaaaaggtta gcacccacac tgaaattgtt
     3601 accatctgca taaataacca tacttgtgta atggtcattt tccagtaaat caatcgcatc
     3661 aactaacgca tcgttgaatt catcggtaat gacattattt ttactttgta atttcagtaa
     3721 cagttgatca tcatgagtta cggaaagttt ggcatcacct ttatcccaaa gttcatcttt
     3781 tacgaagtga gaaataggtg ttgcatattc aatggtctca tcttgtttat aaaagccacc
     3841 atctaaatca ctaatccatt gtggtaagtc tccaagttcg tcttccatac gtgttttaac
     3901 acgttcgtat cccattgcat cccataattg gaatggacca agtttccagt tgaaccccca
     3961 gacaagcgca cggtctatgt ctcggaaatc atcggtagct ttaggtacat tgatagcaga
     4021 gtaatagaaa ttattacgta atgtctccca taaaaatagt cccgcttcgt cttgcgcatt
     4081 gaatatggta tcaaggttat gcactaagtc tttattaaat tcatttaaaa ttggtaattg
     4141 tggttgcgat acaggtacat aatcttgttt ttcaacatcg taaacaagtc gagctttagt
     4201 ttctttatcc tttttgtaaa atccttgttt cgttttacgt ccgagtgcgc cattgtcaaa
     4261 caacgtattt acaattttga catcatgaaa ataaggtgtt tcttcaggta cttgttgcat
     4321 gcctttaatt acagacactg caatatctaa accgactagg tcagatagcg catatgtacc
     4381 tgttttagga cgaccaatcg cttgcccagt taaagcatcc acatctacaa tgcttatctt
     4441 gtgttgctcg gcgcgataca taatatcatt cattgtttgc gtgccgactc tatttgcgac
     4501 aaagccaggc acatcattga cgacaatgac acctttacct aacacatttt gcgcgaaatt
     4561 ttttacatct aatataatag attccttcgt gtgtgacgta ggtattaact ccactaattt
     4621 cataatacgt ggtgggttaa agaaatgtag accaaagaat cgttcttgat ccttctcgtt
     4681 aaatgcttga gcaatcgcat taattggaat acctgatgta tttgtagcga ataaagcatc
     4741 ttctttagca tgttgtagaa cttgttgcca aacagcatgc ttaatttcaa tatcttcttt
     4801 gactgcttcg atatataaat cagcatcatc atttaccaag tcatcatcaa aattaccata
     4861 tgttaaatga ctcgctagat ttaagtcgaa tagtagcggc cgtttcttat ctgtaatttt
     4921 atcgtaagat tttttcgcaa tgagatttgg atcgtttttg tccactacaa tatctaatag
     4981 ttttacttta agtccagcat tcacaaaaag tgctgccagt tgagcgccca ttgtgcctgc
     5041 gccaagaacg gttactttat taattgtcat agtgattcct ccaatttagt tgaggataag
     5101 ataaccatta agataattgg aataacgttg ctattttata aaattaatta agtatctttg
     5161 acagtcatct tagcctctta tttaaggaaa aagctttatg cttaaaataa gtctttttta
     5221 gtgaaattaa tgcatctcat ataattattt gctatttata cgaaagcaga atctccagtc
     5281 aaagcgcgtc caattactaa ggcattaatt tcatgtgtac cttcgtacgt gtaaatcgct
     5341 tctgcatcag agaagaaacg tgcaatatca taatcgtcag ctagtatgcc attaccacct
     5401 gtaataccgc ggcccatagc tactgtctca cgcaaacgta aggcattcat catcttcgcc
     5461 gttgaagttg caacctcgtc atattcacca tgtgcttgca tattagctaa ttgagcacat
     5521 gttgccattg cttgagctaa attaccttgc atcattgcta gcttttcttg tattaactga
     5581 tatttactaa ttggtttgcc gaattgctta cgctcagtga cataatctaa tgtggcacgt
     5641 aaagcgccag ccataccacc tgtagccata taagcaacgc ctgctctcgt tgaataaaga
     5701 attttggcaa tatctttaaa gcttgttatg ttttgtaagc gatccgcttc atctactttg
     5761 acattagtta atttaattag ggcgttagga acaatgcgaa gtgcgatttt attatcaatg
     5821 acttcaatat cgacgccatc ttgttctggt ctgactacaa agcaatgggg tttgccagtt
     5881 tctttattta ctgcgaatac tggaatgaca tcagatacat gtgcaccacc aatccatttc
     5941 ttttcaccat tgataaccca agtatcgcct tggcgttcag cgactgtttc aagacctccc
     6001 gcaacgtccg aaccgtgttc tggttcagtt aaagcaaagc atgtacgcag ttcatgtgac
     6061 tgtaatttag gtacatattt cgcaatttgt tctttgctac ctccgaaata gaaagtgtta
     6121 tgccctaaac cttggtgaac accgagtagg gtagctaagg aaatatcaaa tcgcgcgagt
     6181 aggtaagaca tgaaaaactg aaatagttga ctaggcattt tggcgtttgg acgatccttg
     6241 taaagtaatg gattgttaaa ataatttaat tctcccagat ctttaaaata gtcctcgggt
     6301 acagtagcgt ctatccaatg ttgattaata ttttcacggt acttactttc tagcaatgaa
     6361 tctacttgtt gtaaaaattc gacttcaccg tctgttaaac ctttagcaat actaagtaca
     6421 tcttcaggaa ataatgtttt taagaccgtt tctttttcaa atgtcatata aattcctcct
     6481 aaaaataata tgaatactaa tgtgaaatgc atttaattca aaaacaacac gctttatttg
     6541 taaacgctta cactaaatgt caaaaatttt tatcaccttt aaagtgtttg cgagactttg
     6601 tcattcatca tttgtcgaat cgcaagttta tctggtttct gcgtactgtt taacggcata
     6661 tgtgtcactg gtacatacat tcttgggact ttataacctg ctaaacgact tcgcatatgt
     6721 tgatttaaaa tttcagcgta atgaggttca tcttcgcgaa gtataatggc tgcagcaatt
     6781 gattcaccat attttggatg atcatagcca acgaccacac accggtctac tagtggatgc
     6841 tcagctaaag cattttcgac ttcggatggt aagacatttt cgccaccagt tatgattaat
     6901 tcttttttgc ggtcaataat aaatatatcg ccatcgttgt ccatcttcgc taagtcacca
     6961 gttaataaat atcgaccatg aaatgctttg gcagtctctg ctggtttatt ccaatatcct
     7021 ggcgtgacat ttttagcctt aattgcaagt tcgccaatct caccagtagg tacttcctca
     7081 ccgttatcat caaggatacg tgcatcaacg aacatgactg ctttaccaat actcattggc
     7141 ttacgttttg aattttccgg tgtattaaca agtacaagag gtgcttcagt taaaccatag
     7201 ccgttaataa tgtttatgcc atattgttta aaagctgctt ggatacttgg taatggttgt
     7261 gaaccacctt ggatgatata atccatagct ctaaaatttt caggattaaa attactagca
     7321 cgtagcgtac tataatacat tgtcggaatc atgataataa atgtagggtg atattgtgca
     7381 atcatgtcat tcaattcttc gccgttaaag taacgttgaa gaataagtgt gccacctgac
     7441 attaatactg gtaatacagt atcgttaaac cctaaaacat ggaacattgg tgttgataca
     7501 atcgtaatat agtttgaatt gaacttatac gtcagctcta agtttgcacc gttatgaaca
     7561 aatgattcat atgagaacat cacaccttta ggtgatccgg ttgtaccact tgtataaatt
     7621 aatgctgcaa gatcttgtgg ttcaacaggt gttgcttgaa aaggttggtg ataatctgga
     7681 tttacgattt catcatattg cgccacatca atatccatat gcaataagtt ttggtcaata
     7741 tcggtgagtg aacttaaatg tttttcagca tagaagagca gttttaattg tgcatcttcc
     7801 acaatggctg caatttcttt tgggttaagc cgccaattca atggtaaaaa aaccgcacct
     7861 gttttaaaac aagcaaacaa taaatctaat attgcaatat catttggcgc aaaaataccg
     7921 ataacatcgc cttttttaac accttgagat gttaaataat gtgccatatt atcagcgcgt
     7981 gcattgagtt gttggtatgt ccaagatgtt tgttttgcgt gatcaataac ggcaggcttg
     8041 tcatcatcga agtctgaacg cgtttttatc caatcgaaat tcattagtat acccccttta
     8101 gcttcacttt catactttat gaattgattg tttaagttgt ccccattttt ctttgtaaat
     8161 gctggtatca attaatttta aatgatcagc aataattggt ttaaaagcca tttgattcaa
     8221 aatatcttta tgcaaatcaa gacctggtgc aatttcaatt agtttcaagc cttgattggt
     8281 gagttcgaat actgcacgat cagtaacaaa atagatttct tgctcgagtg attgtgaata
     8341 ttgtgcatta aagtcgatat ggctcacatc tgatacaaat ttctggtttt gtccttcagt
     8401 ttcaatgttt aatcgttgat tatggcatga gacatgactg ccagctacaa aagtacctga
     8461 aaagataatt ttatttacag attgcgtaat gtctataaag ccaccacatc catttagtcg
     8521 gtcattgaag taagacacgt tgacattgcc gtattgatca acctcagcaa agctaagata
     8581 ggcaactgat acaccattgt tataaataaa atcccatgct cgatcatgag gcatgcgcac
     8641 atctgcattg taattcatac caaaatgttc acgactccca acgaatccac cgaaaatgcc
     8701 aacatctaaa atcggttgca catcatgttc aacacattct tcatgcaata aattagagag
     8761 ttcattattg atgccataac cgatgctaat tgtatcgcca taagttaaaa actgagcagc
     8821 acgtcggaga atcaatttgc gactattaaa aggtaatgcg ggttcaggta ttccatcaat
     8881 tcgttcttct ccagacaagg ctggtaaata atgactttga attacttggc ggtgattctt
     8941 ttcatcttct gtgacgtata cataatcgac aagatttcct gggataacaa cttcattcgg
     9001 ttttagttga tagtcgtcaa ctaaagcttt aacttgtaca ataactttcc catgattggc
     9061 tttcgcgttt aatgcgacat gataacactc gctcaagtac gcttcttgag ttaaataaat
     9121 gttaccttgt tgatctgcgt atgttcctct cagtagtgcc acatcaacgc tagggaatgt
     9181 gtaatgtaag tatgtttcat cgttgatggt tactaatgaa actaaatcat ccgttgttcg
     9241 tgtatttact ttaccgccac cgtatctagg atcaacagct gtgtttaatc cgattttagt
     9301 aataactcca ggtaataatt gattactctg acgataatga gttgcaatga taccttgtgg
     9361 taaaaaataa gcttcaatgt cattattttt cattgcttgt gccgttttgg aagaagccgt
     9421 taaaatactc ataatgacac gtttaatcat gcgacgttct ataaaatcat ctaaatccgg
     9481 tgcggcacct aaactatgaa tatcattcgc taatataaac gttaaatcat tgggcgtatg
     9541 atatgtgtca tgttgcgcta acacagcacg tagaacttcg gcgggtaagt tggctacagc
     9601 taatgctggt aaaccaatca catcaccatc tttaatgata tgttgtaagt cgtgccatgt
     9661 gatttgtttc aagcaagtca cctccatcac atttgataaa atatagcgtt tttacacttt
     9721 gtgtaaaccc ttacaagaaa tataacataa cgacgtttaa aatcaattag aaatatcttt
     9781 ttattctgat aatagacaca gtatagacac attttgacgg tcgataacaa ttgtaatatc
     9841 aagggtttgt aatgaattga atatcattaa aatacttata taaaaatatt gttcggaata
     9901 taaaaagtta aataggtttt gatttttaaa tatgaaatac aaagtgccca atcgaacaaa
     9961 gtatttatat taaaatatgg aaaatccatc aatattaaat taaaatagtt ttattatgaa
    10021 aagtgaaagt aggtaagtct atggaaggtc ttaatcatcg aagaaataca gaaaaagaag
    10081 agacaacaca aacgcaatca gttgcaccta atacaggtga agaggggatg tcatcagcaa
    10141 gtacacaatc aactaagacg tccgacatac ataatgaatc tatcgataaa caaatggaag
    10201 ctaaagcgca tgaaacagcg caaaatacag atttaaaaaa cgaagcaaga agtttatttg
    10261 ataatgcaac caaatcaatc ggtagactag cgggcaatga tgaaagctta aatcttaatt
    10321 taaaagatat gctttctgaa gtatttaagc cgcatactaa aaacgaagca gatgaaatat
    10381 ttatagcggg tactgctaaa actacgccag caatttgtga catatcagaa gaatggggga
    10441 agccatggct cttttctcga gtattcatcg ctttcacagt aacatttatt ggattatggg
    10501 tcatggcagc aatttttaat aacactaacg cgattccggg tctcattttt ataggggctt
    10561 taacagtacc attatcgggt ttgttcttct tttatgaatc aaatgcgttt aaaaatatta
    10621 gcatttttga agttattatc atgttcttta ttggcggcgt attttcatta ctaagtacga
    10681 tggtattata tagatttgtc gtttttagtg atcaattcga aaggtttggt tctttaacat
    10741 ttttcgatgc atttttagta ggattagttg aagaaactgg aaaagcactc attattgttt
    10801 atttcgtcaa taaattgaaa acaaataaga ttttgaatgg attattaatc ggtgctgcta
    10861 ttggtgcagg gttcgcagtt tttgaatcag caggttatat tttgaatttc gctttaggag
    10921 aaaatgtccc attattagat attgtcttca cacgtgcgtg gactgcgatt ggtggtcatt
    10981 tagtttggtc agcgattgtt ggtgctgcaa tagttattgc gaaagaacag catggctttg
    11041 aattcaaaga tatttttgat aaacgctttt taatattctt tttatcagcc gttgttttac
    11101 atggcatttg ggatacatct ttaactgtac ttggcagtga tacgttgaaa atatttattt
    11161 taatcgttat tgtgtggata cttgtattca ttttaatggg ggcaggttta aaacaagtga
    11221 atttactgca gaaagaattt aaagaacaac agaaaaaagt agacgaataa taattaaagc
    11281 ttatgttgct catatgtttg tgacataagc tatttttata atttgtcttt aaaagagtgg
    11341 aataggaata ctttttggag ttaaaaaagt gtttcacgtt aaacaaatag tgacaattag
    11401 atttatataa aatgaacatg attcactgaa agtatgtaat aatcatttta ttgaaattca
    11461 tcaaacagaa attaatacaa tcatataagc aaattaaacc acgccataat catattggat
    11521 gacttcggcg tggtttttat agttgaagca gggctgagac ataaatcaat gtcccacact
    11581 cccttatcgt tcaatcgttg ttcgataatc gattaaatag ataccttcag gtgttacttt
    11641 ataattttta accttagagt tagcagcgac tatttgatcg ttgtaagcaa tataactgtt
    11701 tggtacatct cgacttgata atttaataat atcattagaa atattgtgac gttccttaac
    11761 atctacagta tgattcaatt gattaattaa atcatcgacg ttgctattat tgtagtctcc
    11821 tttattaata gcaccatctt ttttatatgc ttgattaaag aaataacctg tatctccacg
    11881 aggaattgtt ccgaaactat acatcgttgc atcccatgca gaacggtctt ttaagtaacc
    11941 ttctatgtca tcaacacttt taatgtcgat ttcaatattt gcttttttag catctgattg
    12001 taatacttgc gcaattttcg atagctctgg acgaccgtca tacgtaatta acttaatttt
    12061 taaagggtgt tcttttgtat aaccatcttt agctaataac atttttgctt gttcgatatt
    12121 ttgtttggtt aacttaggtt ctttaatata tggaatttta tcattaaatg gactcgttgc
    12181 aggtttcgca taaccttgat aaatatgatc tgcaatacct tgtctatcaa tgatatgatc
    12241 taatgcttca cgaacggatt tagtcatttt tttattagta tgattataca taagtaaaga
    12301 agttctaaat ccagattctt ttgacacttt taaattttga ttattttcta tgtcttgaac
    12361 tttattaact gggacatcag ttattaaatc atctttttga gattctaaat ttctgacgcg
    12421 attattgccg tcttcttggt acgtcacagt aatatgatca agtttcggtt taccttgcca
    12481 atagtcctta aaattcgaca atgatatttt tcgagattgc ttataatctt ttatttggta
    12541 agggcctgta ccaacaggag tttgattaac atctgattta gcatctgtat cataaattgc
    12601 cataaaagga ttagctaatt cagatacaag ttcagggtaa gcggagttgg ttttaattgt
    12661 cagtttttga cctttagcgg taattgatga tattggtaat gaatatttga ccaagtcgct
    12721 ttttttcatg ctattttcaa ggctagattt cactttttct gcagtcaatt tttgaccgtt
    12781 ttgaaattta atattatctt ttaattctat atctaacgtt gtatcatttg gttgatgata
    12841 cgatttcact aatgcttttt ctatttttcc ttgatcattt gttttaaata atgattctgc
    12901 agcaccaatc ttaactggta catctgtttc ataaggtgca atagactttg tttttaacgg
    12961 taacgaaata tttaagtctt tgccagatga atgcattgag ccacatcctg ataacactaa
    13021 tactgctgaa aatatagttg ctagtctttt aaacttcatt tcattaacac tctctttcta
    13081 attactatgt aaaacccaac aattaatatt ttaaaacttt attttgttaa agtaaaatgt
    13141 tgttca
//
LOCUS       DAMQYT010000059        12646 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMQYT010000059 DAMQYT010000000
VERSION     DAMQYT010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12646
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.059"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(231..431)
                     /locus_tag="PGA33_001255"
     CDS             complement(231..431)
                     /locus_tag="PGA33_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HDG6473075.1"
                     /translation="MWIVISIVLAIFLLILLSSISHKMKTIEALEYMNSYLFKQLVKN
                     NGVEGLEDYENEVERIRKRFKS"
     gene            complement(499..651)
                     /locus_tag="PGA33_001256"
     CDS             complement(499..651)
                     /locus_tag="PGA33_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDG6473076.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYEKI
                     RAEVSW"
     gene            complement(648..1034)
                     /locus_tag="PGA33_001257"
     CDS             complement(648..1034)
                     /locus_tag="PGA33_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473077.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGIFALLLVITLPIYTVASYQHK
                     ELHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKVE
                     VKTIGYRIHFLNLYPVLYEVKKVDKQ"
     gene            complement(1027..1263)
                     /locus_tag="PGA33_001258"
     CDS             complement(1027..1263)
                     /locus_tag="PGA33_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473078.1"
                     /translation="MINIPKMKFPKKYTEIIKKYKNKAPEEKAKIEDDFIKEIKDKDS
                     EFYSPTMANMNEYELRAMLRMMPSLIDTGDDNDD"
     gene            complement(1256..1459)
                     /locus_tag="PGA33_001259"
     CDS             complement(1256..1459)
                     /locus_tag="PGA33_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473079.1"
                     /translation="MTEVKIKTISDRVYYTTTDLASDDYINLVMNLVIEDFLPVKDVF
                     NNEVWVKRDEIESFTFIKEANDD"
     gene            complement(1456..1662)
                     /locus_tag="PGA33_001260"
     CDS             complement(1456..1662)
                     /locus_tag="PGA33_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HDG6473080.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAERKY
                     EAQVKRDAVIKVGQLFENIRECGK"
     gene            complement(1699..2235)
                     /locus_tag="PGA33_001261"
     CDS             complement(1699..2235)
                     /locus_tag="PGA33_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HDG6473081.1"
                     /translation="MTNTLTIDQLQELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWF
                     NTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKNSEF
                     HNMFNFKDKEFAQDAVVSTPQIIFKEFYPDQLAIVIAIDIAFNLYSIDQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            complement(2228..2476)
                     /locus_tag="PGA33_001262"
     CDS             complement(2228..2476)
                     /locus_tag="PGA33_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDG6473082.1"
                     /translation="MNNREQIEQSIISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEDQEND"
     gene            complement(2491..2733)
                     /locus_tag="PGA33_001263"
     CDS             complement(2491..2733)
                     /locus_tag="PGA33_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473083.1"
                     /translation="MTDSARKERLNQFFGSKRYLYQDNERVAHTHVVNGTYYFHGHIV
                     PGWQSVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            complement(2748..2948)
                     /locus_tag="PGA33_001264"
     CDS             complement(2748..2948)
                     /locus_tag="PGA33_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473084.1"
                     /translation="MATQKQVDYVMSLQEQLELEDCEKYTDEQVKAMSHKEVSNVIEN
                     YKTSIRNEELYYECMSFGLPNC"
     gene            complement(2951..3208)
                     /locus_tag="PGA33_001265"
     CDS             complement(2951..3208)
                     /locus_tag="PGA33_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HDG6473085.1"
                     /translation="MSIISNRKVDMNETQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVDRVFDLWEG"
     gene            complement(3208..3609)
                     /locus_tag="PGA33_001266"
     CDS             complement(3208..3609)
                     /locus_tag="PGA33_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVL family protein"
                     /protein_id="HDG6473086.1"
                     /translation="MARRKVIRVRIKGKLMTLREVSEKYHISPELLRYRYKHKMRGDE
                     LLCGRKDSKSKDEVEYMKSQIKDEEKEREKIRKKAILNLYQRNVRAEYEEERKRRLRP
                     WLYDGTPQKHSRDPYWFDVTYNQMFKKWSEA"
     gene            complement(3609..3794)
                     /locus_tag="PGA33_001267"
     CDS             complement(3609..3794)
                     /locus_tag="PGA33_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HDG6473087.1"
                     /translation="MQHQAYINASVDIRIPTEVESVNYNQIDKEKENLADYLFNNPGE
                     LLKYNVINIKVLDLEVE"
     gene            complement(3807..5759)
                     /locus_tag="PGA33_001268"
     CDS             complement(3807..5759)
                     /locus_tag="PGA33_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HDG6473088.1"
                     /translation="MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAI
                     DMTKVDNEPFHADFETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMV
                     NSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLE
                     KWQQFIDYCIRDVEVEMTIAHKIKDFPVTAIEQAYWVFDQHINDRGIKLSKSLMLGAN
                     VLDKQSKEELLNQAKHITGLENPNSPTQLLAWLKDDQGLDIPNLQKKTVQEYLKEATG
                     KAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLT
                     KHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFS
                     AIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELA
                     LGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRK
                     THHTHGVRFYMKKGFLMIELPSGRALAYPKASVGENSWGSQVVEFMGLDLNRKWSKLK
                     TYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETI
                     MNKPVDWAKGLNLNSDGFTSPFYMKD"
     gene            complement(5827..6384)
                     /locus_tag="PGA33_001269"
     CDS             complement(5827..6384)
                     /locus_tag="PGA33_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HDG6473089.1"
                     /translation="MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQESKYSISLIIP
                     KSDTSTIKAIEQAIEAAKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFIN
                     ASSKQAPGIIDQNKIRLTDSGTVVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEK
                     GEPLGGASAAEDDFDELDTDDEDFL"
     gene            complement(6410..7576)
                     /locus_tag="PGA33_001270"
     CDS             complement(6410..7576)
                     /locus_tag="PGA33_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HDG6473090.1"
                     /translation="MKLDHSNRAHAKLSASGAKQWLNCPPSIKASEGIADKSTVFAEE
                     GTFAHELSELYFSLKYEGLTQFEFNKAFQNYKRNQYYSEELREYVEEYVANVEEKYNE
                     ALSRDNDVIALFETKLDLGKYVPESFGTGDVIIFSGGVLEIIDLKYGKGIEVSAIDNP
                     QLRLYGLGAYELLSLMYDIHTIRMTIIQPRIDNFSTEELPISRLLQWGADFVKPLARL
                     AYNGGGEFKAGSHCRFCKINHSCRTRAEYMQNVPQKPPHLLSDEEIAELLYKLPDIKK
                     WADEVEQYALNQAKENDKNYPGWKLVEGRSRRMITDTKAMLEKLVEAGYKPEDITETK
                     LLSITNLEKLIGKKAFSKITEGFIEKPQGKLTLATESDKRPAIKQSAEDDFDKL"
     gene            complement(7573..7935)
                     /locus_tag="PGA33_001271"
     CDS             complement(7573..7935)
                     /locus_tag="PGA33_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473091.1"
                     /translation="MAILEDIFEELKLLNNNLRVLNTELSTVDSSIVQEKVKEAPMPK
                     EETAQLESIEEVKETSADLTKDYVLSVGKEFLKKADTSDKKEFRNKLNELGADKLSTI
                     KEEHYEKIVDFMNARINA"
     gene            complement(7949..8209)
                     /locus_tag="PGA33_001272"
     CDS             complement(7949..8209)
                     /locus_tag="PGA33_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDG6473092.1"
                     /translation="MYYKFGEIKNKIISFNGFEFKVSAMKKHDGISIQIKDMNNIPLK
                     SFHVVDLSELYFATDAMRDVINEWIEENTDEQDRLINLVMKW"
     gene            complement(8214..8516)
                     /locus_tag="PGA33_001273"
     CDS             complement(8214..8516)
                     /locus_tag="PGA33_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HDG6473093.1"
                     /translation="MTKKYKDMTQEEVKDLLSEKSGELYELAKEIKGESKFDILLFSS
                     IGVIDGDYLAGSSSVIGHTFDLASLLDSTKSYKDIVNVLQMCKSQKFHGIDDNKEG"
     gene            complement(8509..8826)
                     /locus_tag="PGA33_001274"
     CDS             complement(8509..8826)
                     /locus_tag="PGA33_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473094.1"
                     /translation="MAENIKTEQHYYTKDFSGYRNEEDNFVANQELTVTITLNEYRKL
                     IEIKAVKDKEEDTYRGKYFAEERKNEKLEKENIKLKNKIYELQNEEDNEEDEENEEEV
                     NND"
     gene            complement(8919..9080)
                     /locus_tag="PGA33_001275"
                     /pseudo
     CDS             complement(8919..9080)
                     /locus_tag="PGA33_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
     gene            complement(9092..9355)
                     /locus_tag="PGA33_001276"
     CDS             complement(9092..9355)
                     /locus_tag="PGA33_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDG6473095.1"
                     /translation="MPKIIVPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKYYRKDNLGVENLYIDYSPTGTLINISKLEEYLIRKHKKWY"
     gene            complement(9380..9595)
                     /locus_tag="PGA33_001277"
     CDS             complement(9380..9595)
                     /locus_tag="PGA33_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473096.1"
                     /translation="MNKKSEGLDIIIPRIFRRDHASVESLTENERRLRKEILGSIKKG
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            9642..9881
                     /locus_tag="PGA33_001278"
     CDS             9642..9881
                     /locus_tag="PGA33_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473097.1"
                     /translation="MISIHKRLLTQYLDKEIVTSLDLHLINGEVIKVQEHIKDAESKT
                     LHIIHPKDRVVSLDHVLYFDINVKGEKNNDSPYPS"
     gene            complement(9878..10054)
                     /locus_tag="PGA33_001279"
     CDS             complement(9878..10054)
                     /locus_tag="PGA33_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473098.1"
                     /translation="MNEEKLKMILLLLEDIPRDEWNRLVNEVNNQYSYQADKVGLASD
                     NCQQIANNYKHYGF"
     gene            complement(10138..10329)
                     /locus_tag="PGA33_001280"
     CDS             complement(10138..10329)
                     /locus_tag="PGA33_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDG6473099.1"
                     /translation="MPEQLSVRKWRLVRDLKQQEVADILGVNAKTVGHWEKDDTNLSN
                     VTVYALAKLYDIEVDQIKV"
     gene            10490..10819
                     /locus_tag="PGA33_001281"
     CDS             10490..10819
                     /locus_tag="PGA33_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDG6473100.1"
                     /translation="MENNKVRKILSENLQELMNDKNIDQRELAEAIGVSQPTVSNWIQ
                     QTKYPRIKRIQQLADYFNVPKSRITESKKDIHQETIAAHFDKEGLTEEEIEEVNRFIE
                     WVRNRDK"
     gene            10832..11293
                     /locus_tag="PGA33_001282"
     CDS             10832..11293
                     /locus_tag="PGA33_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDG6473101.1"
                     /translation="MGLYEELCINNEKIKIEETDQLPNFQPGCYMNGKIYIRRNLSEV
                     RKAEVLYEQLAHHKLTYGNILDQTKWINRKFENYARRHGFTSAVPLHEIVEAHNYGVR
                     NLYELSEYLQLSESYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            11311..11745
                     /locus_tag="PGA33_001283"
     CDS             11311..11745
                     /locus_tag="PGA33_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473102.1"
                     /translation="MKKVIGLLLVSTLALTACGEKEKPKKEENKKSQTQNHKDSKPKT
                     QQEKMKKVEDKNPPNNSIQNNSNNQNQSQNNQLNNNSDPSNNTPANINENDSQNTNLN
                     DEYVVSPGWTKDEQAKAFEEYKKGKEEEARAGASAVPGANIN"
     gene            11774..12169
                     /locus_tag="PGA33_001284"
     CDS             11774..12169
                     /locus_tag="PGA33_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473103.1"
                     /translation="METNKTIDLMNYVEFPKRYTEAKGKLVAQPITTINSARRVENED
                     MTVCYILDQDDDVMDFIFDRDIITVYCPENGTATDEYFCEIIFNSDDTFTLKRLSNYV
                     TIKDRSYPMSKINDVNITGKVVRLFRDFK"
     gene            12259..12405
                     /locus_tag="PGA33_001285"
     CDS             12259..12405
                     /locus_tag="PGA33_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473104.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
     gene            complement(12402..>12646)
                     /locus_tag="PGA33_001286"
     CDS             complement(12402..>12646)
                     /locus_tag="PGA33_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDG6473105.1"
                     /translation="LFNNNGKPVFIVIKDFENRIIEEGELKTYNSAGSDFDLLEVERQ
                     DFKVSDLPSNDELYIKHTLVDLKQQIKLDLYLMNEY"
BASE COUNT         3841 a         2399 c         1770 g         4636 t
ORIGIN      
        1 aaaactttac gcgaaatcat ttatgataaa tacgaaaatg aaaacttgag aactttaatt
       61 ttattgcact caggaaaagc ctttcccgta actaatataa tctcagaaaa ttcaaccttc
      121 ttaattatca agacttcaaa gacaatttca cataaattag ttttaattta caaaaacgaa
      181 ataaaaaata tccgcttctt ttaatcctgg agagggcttt agccccctct ttagcttttg
      241 aatctttttc taattcgttc aacttcattt tcataatctt ctaaaccttc aacaccatta
      301 ttttttacta actgcttgaa aagataagaa ttcatatact ccaatgcttc tatggttttc
      361 atcttatgag aaatgctact taacaagatc aataaaaata tagctaaaac gattgaaatg
      421 acaatccaca tatttacaac acctccagtg ctattgctaa acacattaat ataattaatt
      481 caaaaatgat aatagctatt accatgaaac ttcagctctg attttttcgt aatcactcgg
      541 cgcctctaca tcatcattag ccgtcatcat aatatacact tgctcagtta catacttacc
      601 taactcatac attgctagta agaataatag tcttagtatt tgtttaatca ttgtttatct
      661 accttcttta cttcgtataa gaccggatat aaatttaaaa agtgtattct ataaccgatt
      721 gttttaactt ctaccttatc gcctactttt aacctagctt gtatatctgc gctatcaaat
      781 ttctttttga ataataagtc ggaattttca atgacttgtt tgttgtctaa tacaatatag
      841 aacttgtctt ctttatcttg tctcttgtta tatttatctg taatagttcc ttgatgtaat
      901 tctttgtgtt ggtaactagc cactgtatag ataggcaatg tgataacaag tagcaatgcg
      961 aatataccga ataatgacag tattccaaca ataaagatgt cgaacccatc catattttta
     1021 agttttttaa tcatcattgt catctccagt atcaattaaa ctaggcatca ttcttaacat
     1081 agcccttaat tcatattcat tcatattagc catcgtagga ctgtaaaatt cactgtcttt
     1141 atctttaatt tctttaataa aatcatcttc aatcttagcc ttttcttcag gtgctttatt
     1201 tttatatttt ttgattattt cagtgtactt tttcgggaat ttcattttag gtatgttaat
     1261 catcgtttgc ctccttaata aatgtaaatg attcaatctc atctctttta acccatactt
     1321 cattgttgaa cacatctttg accggaagaa aatcctcaat cactagattc ataacaagat
     1381 taatataatc gtcagaagct agatctgttg ttgtgtaata aactctatct gaaatagttt
     1441 taattttaac ctccgtcatt tcccacactc ccttatattt tcaaataact gacccacttt
     1501 aataactgca tctcttttaa cttgtgcctc gtatttgcgc tcagcttctt ctttactctc
     1561 tgcctcaaca actgtaaacc tttgattgct tttagcttta gttatgtgtg tatgcttgcg
     1621 tcctgttgaa tctttgaatg ttgtgactaa gtattgcgtc acttccccaa aacctccttg
     1681 actcgatcta agatgtcttt acacgtatcc ttttcctgcg tctgctgttc catcttgtct
     1741 ttcatgattc cttttcattt tctttttgta tgcgtcaatg agctggtcga tagaatataa
     1801 gttgaaagct atgtctatcg ctattacaat tgccaattgg tcaggataaa attctttgaa
     1861 tattatctgt ggtgtgctaa caactgcgtc ttgagcaaat tctttatctt taaaattaaa
     1921 catgttgtga aattcactat ttttaaaact tgattcaatc gcttctttta tctcttctga
     1981 tgacactcct acttgattcg caatactcaa tccaaacgcc aacatgtccg ctaattcatc
     2041 taactgaaca tctaacggct tacctggttt cttcttccag ttcttaaacg tttccaatgt
     2101 attaaaccat tcaaagaatt caactacata tgctattttg ctatctcgta agttcagcgt
     2161 tggtattcta tcgtcgaact ccttttgtat ttgtaataac tcttgtaact gatcaattgt
     2221 taatgtatta gtcattttcc tgatcctcct catatttata gaccacttgc cccgtcataa
     2281 tccctactgc ttcatcaaga ccaatatctt ctttgagtgc atcttgcata gcattaggta
     2341 aaccttcaag tatttcatca aacgcttgcg ctttcttata cacgtcttca atctctttta
     2401 gtaatccctc tgtgtcattg ccgttatacg cactagcact gataattgat tgttcaattt
     2461 gttcgcggtt attcatcatt tccatctcct ctaaaataaa gttagttgct tctgttcctc
     2521 gtattccaaa ccatgttgct ttatatatat ttcgagctct tcagcagtat caaatgtctt
     2581 tttaacgctt tgccaacctg gcacgatatg cccatgaaag taatacgtgc catttactac
     2641 atgagtatgt gccactcgtt cgttatcctg atacagatat cttttagagc cgaaaaattg
     2701 gtttaagcgt tctttacgtg cgctatctgt catggtcgtc actcctttta acaattaggc
     2761 agtccaaacg acatgcattc gtaatatagt tcttcattcc ttatgcttgt cttatagttc
     2821 tcaatcacat tgctaacttc tttatgactc attgctttaa cttgttcgtc tgtatatttt
     2881 tcgcagtctt ctaattccag ttgctcctgt aatgacatta cgtaatcaac ttgtttttgc
     2941 gttgccatcg ttacccctcc cacaagtcaa acactctatc gacgtaaaac ttcgcctttg
     3001 ctaaatcctc atgaccattc tttaacggtg ctctagacaa gtatttgatt gcattaccta
     3061 ttgcgaatgc taattgtggt ggatactgtg ccgtaacttg ttcgatgaaa tctataattt
     3121 caatgtcgcc gtatgtgtaa tgcgcaggtt gtttaacatt gtcttgtgtt tcattcatat
     3181 ctacttttct gttactgatt atgctcatta tgcttcactc catttcttga acatttggtt
     3241 ataagtgaca tcgaaccagt acggatcacg tgaatgtttt tgaggcgttc catcataaag
     3301 ccatggtctc aatcttctct ttctttcttc ttcatattcc gctctcacat ttcgttggta
     3361 taggttcaaa atcgcttttt ttctgatttt ttctctctct ttttcttcat cttttatttg
     3421 actcttcata tattcaactt catctttaga ttttgagtct tttcttccac acaataattc
     3481 atcgccgcgc attttatgtt tgtatctgta tctaagaagt tctggagata tatgatattt
     3541 ttctgaaact tctctcaatg tcattagttt tcctttgata cgcactctta taacttttct
     3601 tctagccatc attccacctc taaatctaaa accttgatat ttataacgtt atattttaat
     3661 agttcacctg gattattaaa taaatagtcc gccaaattct ctttttcttt atcaatctga
     3721 ttgtaattaa cactttcgac ttctgtagga attctaatgt caacagaagc attgatataa
     3781 gcttgatgtt gcattcaatc acactcctaa tccttcatat aaaacggaga agtaaatccg
     3841 tcactattca aattcaatcc ttttgcccaa tcgacaggct tattcatgat agtttcgatt
     3901 tccttaagtc catttgaacc tctaggtatt tctacaatta cttcatcatg gacatgtcca
     3961 actattttaa aacctgatgc ttcaagccta gctatagaaa tcgcaagtaa atcccttgca
     4021 gttgcttgaa caatattctc gactaacttc ccaccatacg tttttaactt tgaccattta
     4081 cggttaagat ctaagcccat aaattcaaca acttgactac cccaactatt ttcaccaact
     4141 gaagcttttg gataagctaa agctcttcca ctaggcagtt caatcattag aaaacctttt
     4201 ttcatataaa atctaactcc atgcgtatga tgcgtctttc gggatttcac agtattaatt
     4261 gcagcctctt ggcaagcctt ccaaaaatta actatgttag gatttgcgtt acgccaacta
     4321 tcaactaaac cttgtaattc attttcttca atgcccattt ccaatgcgcc catcgctttt
     4381 aaagctccag cgccaccttg ataacctaaa gctaattcgg acacttttcc cttttgtctg
     4441 agagggtcgc ctttagttat gctttctacc gggacattaa acatttgaga agccgatgct
     4501 tcatatatct ttccgtgtgt gttgaacaca tctaaacgcc attgttcttt tgcataccat
     4561 gctatgactc ttgcctctat tgcagaaaaa tcacttactg ctagttcatt accttcttca
     4621 gcagtaaatg tcgtcctaac taattgactt aataagtctt gaggatgaac attgagtaat
     4681 aaatctaaat cgtcaaaacg ttgttcttta ataagatctc ttgctatttc taattcagta
     4741 tctgaaatat aatgctttgt taaattctga agttgtacac ctctacctgc ccatcttcca
     4801 gtaccggcac cgtaaaattg aaacagacct cttacccgtt catcactgca catcatgtca
     4861 tgcattttgt tgtatttttt cacactggtt ttagacattt gcaatctaat ttctagcatt
     4921 tttttagctt ttcctgttgc ttcttttaag tactcctgaa ccgttttctt ttgtaaatta
     4981 ggtatatcta atccttggtc atcctttaac caagccaata attgtgtagg actattagga
     5041 ttttctaaac ctgttatatg tttagcttga ttaagcaatt cttctttact ctgcttatcg
     5101 agcacattag ctcctaacat caatgattta gaaagcttaa tacctctgtc gtttatatgt
     5161 tggtcaaaaa cccaatatgc ttgttcaatt gcagttactg gaaagtcttt aattttatga
     5221 gcaatcgtca tttctacttc tacatctcga atacagtaat ctataaattg ttgccatttt
     5281 tcaagatcat gttcaggcaa gtttcttgtt cttcctccat taacttttgt tggtttacaa
     5341 ggtatagaga aataacgaat taaattttta cctgctttat ctttttggtt ttgtagtctt
     5401 aaaacttctc caactttatc aagcgaagca ggtaagccaa tacgcattga attaaccatt
     5461 gtgcaaatcc attcttcagg tggcatctgt ttattaaaat gtttagcaag acaagttctt
     5521 tcgaaattag cattgaatgc atactttttt acagcaggat caaaaagagc aattttaaac
     5581 gtctcaaaat cagcgtggaa aggctcatta tctactttag tcatgtcaat cgcactaatc
     5641 gctccaccat ctattgaata agctataatt aaaatttcga aatcttcagc ttctgtgtat
     5701 ttataggcac cacatttcga aatatcatta ctgctatatg tttcaatatc tatattcata
     5761 aatctcaaat tcttgacacc tcaatttctt taaaattaaa gtggggctaa aaccccacct
     5821 attgacttat aagaaatcct catcatcagt gtctaattca tcgaaatcat cttctgctgc
     5881 acttgcaccg ccaagaggtt cgcctttttc tacaagttga atgttgttca atccaactgc
     5941 gataccctta ttaccatttg tgttgaaagg aaatagatta attgaagctc taatataatc
     6001 accacttaca acagttccag aatccgttaa tctaattttg ttttggtcaa taataccagg
     6061 tgcttgtttg cttgatgcgt taataaaata agcgtcttga taattgacat catcttctct
     6121 ttcagtatct ccatcacgta atggaagttt cagatttgca ggaactttgc ctccaaactt
     6181 actaactttc ccttcttctt tagcagcttc tatagcttgt tcaatggctt ttatcgtact
     6241 tgtatctgat ttaggaatga ttaaactgat tgaatacttt gattcttgcc cttcttgcat
     6301 actgtgaggt tcaaaaatat gtgcatatga tgctcttact tttcctgtaa tcactttagt
     6361 tttatttaat acttttgctt tcatgtttat ataccgtcct tttaaatttt tatagtttgt
     6421 caaaatcatc ttcagcagat tgctttatag ctggtcgttt atccgactcg gtagcaagtg
     6481 ttaatttacc ttgcggcttt tctataaaac cctctgtaat tttagaaaat gcttttttac
     6541 caattaattt ttctaaattc gtaatgctaa gtaacttggt ttctgtaata tcttcaggtt
     6601 tataacccgc ttcaactaac ttttcaagca ttgcttttgt atcagttatc attcttcgtg
     6661 aacgaccttc tacaagcttc caaccaggat agtttttatc attttctttc gcttgattta
     6721 gtgcatattg ttctacttca tcagcccatt ttttgatatc aggcagttta tataaaagtt
     6781 ctgcaatctc ttcatcactt aacaaatgtg gtggcttttg aggcacattt tgcatgtatt
     6841 ctgcacgtgt tctacatgaa tgatttatct tacagaatct acaatgacta cctgctttaa
     6901 actctcctcc accgttataa gcaagtctgg ctaatggttt aacaaaatcg gctccccatt
     6961 gaagtaatct tgatattggt aactcttcag tagaaaagtt atctattcga ggttgtatga
     7021 tagtcatgcg aattgtatga atgtcataca ttaaactaag cagttcatat gcgcccaagc
     7081 catataatct aagttgagga ttatctatag ctgaaacttc aatgccttta ccatatttaa
     7141 ggtcaataat ttcaagtaca ccacctgaaa atataatgac atcaccagtt ccaaaagatt
     7201 cagggacgta tttacctaaa tctaattttg tttcaaataa agctattaca tcattatccc
     7261 tactcaaagc ttcgttatat ttttcttcta cattagctac atactcttcc acatattcac
     7321 gcaattcttc gctgtaatat tgatttctct tataattttg aaaagcctta ttaaactcaa
     7381 actgtgttag gccttcatat ttaaggctga aatataactc acttaactca tgggcgaatg
     7441 taccttcttc ggcaaaaact gtacttttat ctgcaatacc ttcacttgcc ttaatactcg
     7501 gtgggcagtt tagccattgt tttgctccac ttgcactaag ctttgcatga gctctatttg
     7561 agtgatctag cttcatgcat ttattctcgc attcataaaa tcaacaattt tttcataatg
     7621 ctcttctttg atagtagata gcttatccgc accaagttcg ttaagtttat ttctaaattc
     7681 tttcttatca gaagtatctg cttttttaag gaactctttt cctactgata aaacataatc
     7741 tttagttaaa tcagcagaag tttccttaac ttcttcaatt gattccagtt gagctgtttc
     7801 ttcttttggc attggtgctt ctttaacttt ctcttgtaca attgatgaat ctactgttga
     7861 tagttcagtg tttaacacac gtaaattgtt atttaatagt tttaattctt caaaaatatc
     7921 ttctaatatt gccattgatt aaatcctcct accatttcat gactaagtta attagtctgt
     7981 cctgttcatc tgtgttctct tcaatccatt cgtttataac gtcacgcatt gcatccgtcg
     8041 caaaatatag ttcgcttaaa tctacaacat gaaatgattt aagtggaata ttattcatat
     8101 ccttgatttg tatactgata ccgtcatgtt ttttcatcgc agacacttta aattcaaacc
     8161 cgttaaagct gataatttta ttttttatct caccaaattt gtaatacatt gttttagccc
     8221 tccttgttat catcaatacc gtgaaatttt tgtgatttac acatttggag aacattgaca
     8281 atgtctttat aactcttagt gctatccaat aaggaagcaa gatcgaaagt atgaccaatc
     8341 acagaacttg aacctgctaa ataatctccg tcgataactc ctattgatga gaaaagcaaa
     8401 atatcaaatt tactttctcc cttaatttct ttcgctaatt catacaattc tccgcttttt
     8461 tcagataata agtcttttac ttcttcctga gtcatgtctt tatatttttt agtcattgtt
     8521 gacttcctcc tcgttttctt cgtcctcctc gttatcttct tcgttttgta attcataaat
     8581 tttgtttttt agttttatat tttctttttc caatttttcg ttttttcttt cttccgcaaa
     8641 atacttacct ctgtaagtat cttcttcttt atctttaaca gcctttattt caataagttt
     8701 tctgtactcg ttcaatgtga ttgttactgt caattcttga tttgctacaa agttatcttc
     8761 ttcatttctg tatcctgaga aatctttagt gtaataatgt tgttcagttt taatattttc
     8821 agccatagtt gactacctcc gtatattttg atttaattaa gttgtatatt ttgataaatg
     8881 tttgtcactg ttacttgttg acgcaagtag cagttttttt attcttcata aaagtattct
     8941 ttgtagtata tgaatgttgc gatacttgcg aatcccgcaa ttgaccatgc tgtagtgaag
     9001 tacagcaatg gcataagcac aatcgctaag actgtgaagc acagtactgc tattaattag
     9061 cttttatatg tgtcgctcat ttaatatcct cctaatacca ttttttatgc tttctgatca
     9121 aatactcttc caatttagaa atattaatca gagtgcctgt tggtgaataa tcaatgtata
     9181 aattttctac acctaaatta tctttgcggt aatatttcaa ccagttgtat actgtacttc
     9241 tacatactcc aaacaattga tggatttgtg taggtgttgc gtataacttt ttcacaaatt
     9301 tttcttcgcc tctatatgtg ttttctggtg ttggtggtac tatgattttt ggcatctcta
     9361 tcactccttt cgataaatgt taaagtttgt tattattcgc tctgtattga agttctctat
     9421 ctaatgcata gaaaactttg tttatttcta agtagctgta atcacctttt ttaatacttc
     9481 ctaatatttc ctttcttagt cgacgttcat tttctgttaa agattctact gacgcatgat
     9541 ctcttctgaa aatccttggt atgattatgt ctaacccttc tgattttttg ttcatttgtt
     9601 gttccacctt tcgtgtataa tgttgttatc aacctaagga ggtgataagt atacataaga
     9661 gattgctcac tcaatattta gataaagaaa tcgttacttc tttagattta catttaatta
     9721 atggtgaagt tattaaagta caagaacata taaaagatgc tgaaagcaaa actctacaca
     9781 tcattcatcc aaaagataga gttgtcagtt tagatcatgt tttgtatttc gacattaacg
     9841 ttaaaggtga aaagaataac gatagtcctt atccatctta aaatccatag tgcttatagt
     9901 tatttgctat ttgttgacag ttatcactgg caagtcctac cttgtcagct tggtaactgt
     9961 attgattatt tacttcattt actaatcgat tccattcgtc tcttggtata tcttcaagaa
    10021 gtaagagaat catctttaat ttttcttcgt tcatttgttg ttcctccttt taagttgttt
    10081 atgtccattt gttacattac ctaaaagtta taaaaagtta tacgaaggtc aaaaaattta
    10141 gacctttatc tggtctacct caatatcata taactttgct aaagcgtaaa ctgtaacatt
    10201 acttaaatta gtgtcatcct tttcccaatg accaactgtc tttgcattga cgcccaatat
    10261 atctgctact tcttgctgtt tcaagtccct tacaagtctc cattttctta cggataattg
    10321 ttccggcatg tcgtccacct ccctttcaca cgtataactt tatattactt tgtattactt
    10381 gtcaacagat aaagttatat ttcttcataa aaagttataa aaagtattgt aaagtaatgt
    10441 cggttatggt aagttaatat tacattaagt aatattaaag gagacaacta tggagaataa
    10501 taaagtcaga aaaattttat ctgaaaacct tcaagaactt atgaatgata aaaatattga
    10561 tcagagagaa cttgctgaag ctattggagt ttctcaacct acagtctcca attggattca
    10621 acaaactaaa tatccacgaa ttaaaagaat tcaacaactt gcagattact tcaatgtacc
    10681 gaaatcaaga attactgaat caaaaaaaga tatacatcaa gaaacaattg ctgctcattt
    10741 tgataaggag ggattaactg aagaagagat tgaagaagta aatagattca ttgaatgggt
    10801 tagaaataga gacaaataaa gggtgtttat attgggatta tacgaagaac tttgcataaa
    10861 taacgaaaaa ataaagatag aagaaactga ccagcttcca aatttccaac ctggatgcta
    10921 tatgaacgga aaaatttata taaggcgtaa tttatcagaa gtacgtaaag cagaagtgtt
    10981 atatgagcaa cttgcccacc acaagttgac gtatggcaac attttagacc aaacaaaatg
    11041 gattaataga aaatttgaaa attatgcacg tagacatggt tttacttcag ctgtaccgct
    11101 acatgaaatt gtagaagctc acaactacgg tgttcgtaat ttgtacgagt tgtcagagta
    11161 tctgcagttg agtgaatcat acatactaga agctatagaa caatataaaa agatatatgg
    11221 tattggaact cactatggcg agtattctat tacgtttgag ccgttgagag tttttaaata
    11281 taaggaaata taaacaaagg agagatacat atgaaaaaag taatcggact gctactagta
    11341 agtacattag ctttaacagc ttgtggtgaa aaagaaaaac caaaaaaaga agaaaataaa
    11401 aagtcacaaa cacaaaatca caaagatagc aaaccaaaaa cgcaacaaga aaaaatgaaa
    11461 aaagttgaag ataaaaatcc acctaataat agcatacaaa ataattcaaa caatcaaaac
    11521 caatcacaaa acaatcaact taataataat tcagatccat ctaataatac tcctgcaaat
    11581 ataaatgaaa acgattcaca aaatactaat ttaaatgatg agtatgtcgt ttcgcctggc
    11641 tggactaaag atgaacaggc taaagctttt gaagagtaca aaaaaggaaa agaagaggaa
    11701 gcaagagctg gtgctagcgc agtaccagga gccaatatta actaataaaa caatataaga
    11761 aagaagagct aatatggaaa caaataaaac aatcgattta atgaattatg tggaatttcc
    11821 aaaaagatac acagaggcaa aaggcaaatt agttgcacaa ccaataacta ctataaatag
    11881 cgcaagaaga gttgaaaatg aagatatgac tgtttgctac attttagatc aggatgatga
    11941 tgtaatggac tttatctttg acagagatat aattactgtt tactgtcctg aaaacggaac
    12001 tgcgactgat gaatattttt gtgaaattat atttaactca gatgacacat ttaccctaaa
    12061 gcgattatct aattacgtta ccattaaaga tagaagctac ccaatgtcaa aaataaatga
    12121 cgtaaacatt acgggcaaag tcgtcagatt atttagagat tttaaataaa cttggcttta
    12181 attacgatta aaagtaccta tatagcgtga cgagaaaaag gattaaaaaa aatcaaaaaa
    12241 cgcctactag tgtagacgtt gaatggtggt gagaatttta tggtagataa aaacaaaaaa
    12301 caagaaacta ctcgtagtaa cccattaaac aaaagttttg aaaagtcagg cgccagcgaa
    12361 aaattaaaaa gcactttatc agaaaaagct aagaaaaaag attagtattc attcattaaa
    12421 tataaatcca atttaatttg ttgtttaagg tctacaagcg tatgtttaat atacaattca
    12481 tcgtttgacg gtaaatcaga tactttgaaa tcttgtcgct caacttctag taaatcgaaa
    12541 tcgctaccag ctgaattata ggttttaagt tcaccctctt caatgattct gttttcaaag
    12601 tcttttataa ctataaatac tggtttaccg ttgttattaa acaact
//
LOCUS       DAMQYT010000060        12260 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMQYT010000060 DAMQYT010000000
VERSION     DAMQYT010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12260
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.060"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(125..292)
                     /locus_tag="PGA33_001287"
     CDS             complement(125..292)
                     /locus_tag="PGA33_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473106.1"
                     /translation="MKRIVILENVIVDNPIWLNHVDYLVKSVLLKSIKLRFQEVRKFE
                     ILVKIMNRIHI"
     gene            complement(420..923)
                     /locus_tag="PGA33_001288"
     CDS             complement(420..923)
                     /locus_tag="PGA33_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDG6473107.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(916..1503)
                     /locus_tag="PGA33_001289"
     CDS             complement(916..1503)
                     /locus_tag="PGA33_001289"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDG6473108.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKGDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGDKNV"
     gene            complement(1515..2315)
                     /gene="racE"
                     /locus_tag="PGA33_001290"
     CDS             complement(1515..2315)
                     /gene="racE"
                     /locus_tag="PGA33_001290"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDG6473109.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(2551..3366)
                     /gene="sdhB"
                     /locus_tag="PGA33_001291"
     CDS             complement(2551..3366)
                     /gene="sdhB"
                     /locus_tag="PGA33_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HDG6473110.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(3366..5132)
                     /gene="sdhA"
                     /locus_tag="PGA33_001292"
     CDS             complement(3366..5132)
                     /gene="sdhA"
                     /locus_tag="PGA33_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDG6473111.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(5184..5798)
                     /locus_tag="PGA33_001293"
     CDS             complement(5184..5798)
                     /locus_tag="PGA33_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HDG6473112.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(6122..7903)
                     /gene="uvrC"
                     /locus_tag="PGA33_001294"
     CDS             complement(6122..7903)
                     /gene="uvrC"
                     /locus_tag="PGA33_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDG6473113.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            8069..8164
                     /locus_tag="PGA33_001295"
     CDS             8069..8164
                     /locus_tag="PGA33_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473114.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(8227..8541)
                     /gene="trxA"
                     /locus_tag="PGA33_001296"
     CDS             complement(8227..8541)
                     /gene="trxA"
                     /locus_tag="PGA33_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDG6473115.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(8714..11062)
                     /locus_tag="PGA33_001297"
     CDS             complement(8714..11062)
                     /locus_tag="PGA33_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDG6473116.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSIKGTKPIFKEDRTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(11072..>12260)
                     /gene="polX"
                     /locus_tag="PGA33_001298"
     CDS             complement(11072..>12260)
                     /gene="polX"
                     /locus_tag="PGA33_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HDG6473117.1"
                     /translation="DQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVKE
                     VAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDEK
                     VSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITIDDINGD
                     IHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIKA
                     LDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLAN
                     ACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADIV
                     RKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNTK
                     LKK"
BASE COUNT         3698 a         2386 c         1712 g         4464 t
ORIGIN      
        1 acattaacta tttattaact aaacattaat atttgtgtca ctacatttct gaatttcaag
       61 aattttgcta ctccatattt taaccatcaa attattgata taatttatga ctttagaaat
      121 ccttttaaat atgtatacga ttcatgattt taactaaaat ttcaaatttc ctgacttctt
      181 ggaatcttaa ttttatactt tttaataata cacttttaac caaataatca acatgattca
      241 accatatagg gttatcaaca atcacattct ccaaaattac aattcttttc ataacataaa
      301 tgtgttattc attccaatta tcttataaaa ttgtcagaaa tacttttaaa caaacattca
      361 tgacacgacc tcctagttaa atcagtctat aaactaaaaa aagccatagc tacttctatt
      421 taaaaagtag tatgactgat tgtttcgtga tgtcgatttt tgaaattgat ggtgccatgt
      481 aaagtttgat catcaataat aacttcagca tatgtttctt ccattgaact tctagattga
      541 gatatacttc caggattaat aacatgaaca ccattaatat actcatattt tgccacatgt
      601 gtatgtccat aaaatgcaaa caaacaacct aattcaagtc ctttttcagc taataaatct
      661 cttgttcgat tgacttgata taaatgtcca tgagtataaa atgctttcac gtcattatat
      721 tttgcgaccg cttcattttc aaattctggg taaaaatcac aattgccttt tactctatta
      781 aataagctaa gttccgtatc atcatacgcg aattctgaat ctcctaaatg taaatataca
      841 tctgcatctg ggtgcatttc ataaatttga tataaaacgc ctgattcagt atggttatca
      901 ctcacaataa tccatttaga catttttatc accttcaaga aaagcttgaa gtaaattaat
      961 cgcatttcgt ctatgactaa tttgcccttt ttgttctttt gaaagttgag ccatggtttt
     1021 atctaatttc ggtacataaa atatcggatc atatccgaaa ccattttcgc catattttcc
     1081 atctgcaatt tcacctgaaa cagtaccttt aaatactttt gtttccatat cagggccact
     1141 catacttatg acacaaacaa attgcgcacg acgatcagtt gtattaccaa gcttatttaa
     1201 taatttttca atatttgctt catcgccttt attttcacca gcataacgtg cagagtatat
     1261 acctggctca ccatttaatg caaaaacttc tagtccactg tcatcagcta tgaccgtttt
     1321 attcaatgct tttgcagcag cttctgattt taatatagca ttttcttcaa atgttgatcc
     1381 tgtttcttcc acatcaaaat ctggtattag ttctgaaata cctattacgt ggtaatctgg
     1441 aaatattact ttaaagtcat ttattttccc ttgattattc gatgcaataa caatctcttt
     1501 cattaaaaat cctcctagtc attcactgat atacgttcca cattgacaga taaatttaac
     1561 cactctttga taatgttagt aatgtgagta ggatcacctg ttgcaaaaaa tcgatgatct
     1621 ggatgttcag tataacttgc atgttcatta ctaaatgtta gcaatgcact aacttcacga
     1681 gccgtttcta atccagacga aatcactgtc tttttaccac caaaataatc atagataggt
     1741 ttatagagca atggatagtg ggtacatcct aaaatgacag tatcagactc actattacgc
     1801 caacgtttca gtgtttgatg aatgacaatg cttgtaattg ttggatcact atatctcatt
     1861 tgttctacaa gtggcacaaa acctggacag gcaacgccat gtacctctac atgtggattg
     1921 atacgtttaa tatgcgtacg atatgcttca gatttaattg tgccttccgt tcctagtact
     1981 aatacatttt gatttctagt agtcattatt gctgttctag cacctggttc aattacgcca
     2041 atcactggga ttgataaggt cttttgtaaa tattctaaag ctacagcagt tgctgtatta
     2101 caagcaatca cgagcatttt tatatcaaat tccattaatt tacgagcgat ttcaactgta
     2161 tattgtttta cttgttctcc tggtcttggc ccatatggac atcgtccaat atcacctaag
     2221 taataaatcg tctcatttgg caactgacgc ataatttctt tagctactgt caaacctccg
     2281 acaccagagt ctattacacc tattggttta ttcatattcg gtcatcctta cttttaattt
     2341 aatttttagt ttatcataac taagcattgg attttagtat tatgcactgt gtttaccatt
     2401 tttgtcatta taatatttat tttaaatcag cccactatca tattgtcatg taatctgctt
     2461 attaaaaaaa tcccttccaa gttattgtgt atctccattc aatttaattt tgaaaggaac
     2521 ataacatttt aactcaaaag ggattaattt ttattctact tcatggtctg aaccaaagaa
     2581 tgatttaaac atgtggaatg ttgtttctct gttcattgct gcaatggatg ttgttaatgg
     2641 aataccttta gggcaagcat taacacagtt ttgtgaatta ccacactgct gtaagccacc
     2701 agtacccatt aatgcattta aacgttcatc tttagtcata gatcctgttg ggtgcaaatt
     2761 aaacaaacga acttgcgaga ttgcttgtgc accaacgaat ttattatttt cagtaacatt
     2821 aggacaaacc tctaaacata caccacatgt catacattta gataattcat aagctgtttg
     2881 acgttttttc tctggcatac gtggtcccgg acctaaatca tacgttccat caattgggat
     2941 ccatgctttc atacgtttta agttatcgaa cattctagaa cgatcaactt gtaagtcacg
     3001 gataactggg aaagtattca ttggctctaa acgaataggt tgttctaatt gatcaacaat
     3061 cgcagaacaa gattgtcttg cacgaccatt gataaccata gaacatgctc cacatacttc
     3121 ttctaagcag ttcatatccc agacaacagg tgttgttttt tcacctttaa tattaactgg
     3181 gttacgtcta atttccatta aacaagcaat gacgtttaaa ttttcacgat atggaatttc
     3241 aaatgtttct tcataaggct tagaatcact tgtatcttgt cgtttaataa ttaattttac
     3301 tgttttttgt ttcggtttag attgtgtttc atgttgtgga gtgtttttca ctgattgttc
     3361 agtcattatt ttttaccccc tttagactta cttgtgtaat cacgtttacg aggtggtatt
     3421 aaactcacat cgacgtcatc ataagtaaac tgcggttttt caaatgcgcc ttggaatgag
     3481 gccattgtcg tttttaacca ctcttcatca ttacgctctg ggaattctgg tttataatgg
     3541 gcaccgcgtg attcgttacg gttatatgca ccaatcgtaa taacacgtgc aagtactaac
     3601 atgttccata gttgacgggt aaagaatacc gcttggttac tccaagtttg agtatcttcc
     3661 atatcaatat cttcataacg tttcatcaat tcaacaatct ttttatctgt ttctaacagt
     3721 ttttcatttt cacgaacaac agttacattt gctgtcataa tttcaccaag ttcacggtgt
     3781 aatttatatg cattttctgt accgcgcata gctaataatt tatcaaaacg ttcttgctct
     3841 tcagctttac gcttttcaaa aatactttcg tccatatcag tatatgatcg atcaatattt
     3901 gaaatataat caatcgcgtt tggacctgct actgtaccac cataaatcgc tgataacaat
     3961 gaattggcac ctaagcggtt accaccatgt tgagagaagt cacattctcc agctgcaaat
     4021 aaccctttaa tatttgtcat ttgatcataa tctacatata gaccacccat tgaatagtga
     4081 acagctggga aaatcttcat tggtactttg cgtgggtcat caccagtgaa tttttcataa
     4141 atctcaatga taccacctag ttttacatct aactcatgtg gatctttatg tgacaaatca
     4201 agatatacca tgttttcgcc atttatacct aatttttggt taatacatac atcgaaaatt
     4261 tcacgcgttg cgatatcacg aggtactaag ttaccataat caggatattt ctcttctaag
     4321 aagtaccaag gcttaccatc tttatatgtc caaattcgtc caccttcacc acgtgctgat
     4381 tcactcatta gtcgcagttt atcatcacca gggattgcag taggatgaat ttgaatgaac
     4441 tcaccattag cataaatagc gccttgttgg taaacaatgg aagccgctga tcctgtatta
     4501 atcattgagt ttgttgtttt accgaaaata ataccagggc cacccgttgc cataataact
     4561 gcatctgaac caaatgtttc aatctcagca gttgtcatat tttgtgcaac gatacctctt
     4621 gcactatcat cgtcaccttt aactatgcca aggaattccc atccttcata cttcgtaact
     4681 aatccatcta cttcatatgc acgaacttgt tcatccaatg catataataa ttgttgtcca
     4741 gttgttgccc ctgcatatgc tgttctgtga tgtaatgtac caccgaaacg tctaaaatct
     4801 aatagacctt catttgttct attgaacatt acgcccatac ggtctaataa atgaataatt
     4861 ttaggtgctg cctctgtcat cgctttaaca ggtggttggt ttgcaaggaa atcgccacca
     4921 tacactgtat catcaaagtg aatccaagga gaatcgcctt cccctttagt attgaccgca
     4981 ccattaatgc caccttgggc acaaacagag tgcgaacgct ttactggtac aactgagaac
     5041 aaatctacat gtgcaccttt ttctgccgct ttaattgttg acattaagcc cgctaggcca
     5101 cctccgacaa caataagatg tttctctgcc ataaaaattt cactccccta aattttcaat
     5161 ctatatttgt taaatgcgat gtattacata aaggcaataa ttgcagtaac accaatatac
     5221 gaaataacta aaaatacgat taatgaaacc catgtaaata ctcgttgtga ttttggagat
     5281 tgaagtccac cccaagtaac taagaatgac cataagccat ttgcaaagtg gaacacaaca
     5341 gcaataatac aaataatata aaatattgcc catccaggat gttgcaatgt ttcgtgcatt
     5401 aaatcgtaat tcacttcttt gccgtaaaat gctttttgta aacgtgtttg ccataaatgg
     5461 ataccaataa agataaatgt taagatacca ctcactcttt ggaagaagaa catccagttt
     5521 ctaaaaatcg agtaatgtcc aacattttct tttgctgtaa atgcaatgtg tataccaaac
     5581 aaaccgtgat ataacaacgg aatgtatata aataaaaatt ctacaataat tagaaatggt
     5641 aatgattcca taaagttaga tgccttatta aacgcttcag caccttgtgt tgcttggtga
     5701 ttcactaata aatgaacgac caaaaatgca cctattggga taatacctaa taacgagtga
     5761 atacgtctta gataaaattc attttttgat tgagccaaaa ggagtccccc ctgtgaacga
     5821 atatttaatt tattgagcta tttatattaa acgtacgctt aaccccctaa agtgatatcg
     5881 tttctagcgt tgtcattatc attaagcgaa acattttaaa gacaaataca cactgcacga
     5941 tcaccaaact gcatgtcgaa caatgtaaca tttggattcg atatttaaaa ttgcttgtga
     6001 tgataaactt tctcatttag aaaacgcttc cacgtacatt caaaaaaata actttgttaa
     6061 ccatattgta acattatttc atatattttg gggcatgaga atgattctca cgcccagtaa
     6121 tttatttatg caattgttca tgtaggttct ttgcgacgtt ttcaggaata cctatatttt
     6181 taaaatcttc aagtgtagct tccttcattt tcttgattga accgaatgaa cgcaataata
     6241 atgttttacg tttgttaccg ataccatcta tatcatcaag tattgatttc aagcctgtct
     6301 tttgacgtgt ttgtctatga aatgtgattg cgaatctgtg aacctcatct tggatacggt
     6361 gcaacaaata aaatgcctgg ctatttttct tcagtggtac aatttctgca ctagcgccat
     6421 ataataattc agatgtttgg tgtttatcat ttttctgcaa acctgcaaca gggatatcaa
     6481 gacctaattc gttttgtagc acatcaataa ccccgttcat atgtccttta ccaccatcta
     6541 ctattattaa atcaggtaat ggtaatcctt cgtttaaaac gcgagaatat cgtcgtctta
     6601 ctacttctct cattgatttg taatcatctg gacctttaac cgttttgatt ttatactttc
     6661 tataattttt cttatctggt ttaccgtcga caaatgtaac cattgctgac actggatcca
     6721 caccttgaat attagaatta tcgaatgctt caattctaat tggtgtttga attcccattt
     6781 gtgttccaag ttcttcaata gctttaatcg ttctggactc atcacgtgat attaattcaa
     6841 atttattatt taaggatact ttagcgttat gtgcagctag gtcaaccata tcttttttgg
     6901 gacctcgcgc gggttgaacg attttagtgt ccacaacaga ttgaatcatt tctttatcca
     6961 aattacgtgg tacatgaact tccttaggta aaatatgttg gtttaagcta taaaattgtc
     7021 caataaatgt ataaaattct tcttcttctg tttgctgtaa tggaatcatc gttgtatctc
     7081 gctttatcat attaccttgt cgtataaaga aaacttggat acacatccat cctttatcaa
     7141 cactataacc aaagacatca cgaatcgttt tatctgatga cataattttt tgtttgtttg
     7201 tcagattttg aatatgttga attaaatctc tatattcttt agcccgttca aaatcaagtg
     7261 attcacttgc agttaacatt cgctcttcta aactttttaa aattgttttg tcttccccat
     7321 tcagaaaatc agtaatttcc ttcgtcattt gtgcgtattt actcaaatca acgtcatata
     7381 cacatggtcc taaacattgt ccaatatggt aataaagaca taatttatct ggcatcttat
     7441 cacatttgcg atatggatat attctgtcta ataacttttt agtttcttga gcagaatatg
     7501 cattcggata cggtccgaaa tatttgccag taccttgttt tacagttctc gtcactagta
     7561 gtctaggata tttctccttc gtaattttaa taaatggata acttttatca tcctttaata
     7621 atatattata tcttggttga tattgtttaa tcagattcaa ttccagtaaa agtgattctg
     7681 tttcacttga cgtgacaata aactcaaagc gacgtatttc accaaccagt ctcgttgttt
     7741 tagcatcatg agcacccgta aaatatgatc gcaatcgatt tcttagcttt ttagctttgc
     7801 caacatatat cacttgatca ttacgatctt tcattaaata gcatcctggt tccataggta
     7861 cgacatttaa tttattttta attcgttgct tatagtcttc caaaaatatc cccccttcta
     7921 attttaaaat gaatgatgct acaaaagtgc ttcaaattta tcaccactaa ttctagttaa
     7981 taatcaacat cttcgagtat gacttttcat gcacatcatg taatattgaa catggtttta
     8041 tcaaaccaac tgctacaaat ctatcagcat gctagattat catttaacgt tcatagattt
     8101 attaatccca ccattctcaa gtcgtaaaca atatgatgac ttatcattca actttttata
     8161 ttaaaaacac aaacaaaaac tggagcaatt cattaaagaa atgccccagt cgtcattggt
     8221 tgtaacttat aaatgtttat ctaaaacttc agctaagttt tcttttggtt ggaaaccaac
     8281 aactttatca actggttgac cgtctttaaa gacgattaat gttggaatac tcatcacttc
     8341 atatttagct gcagttgatg gattttcatc aacatctaat tttaaaatgt cagctttacc
     8401 ttcatagtca gctgctaatt cttctaatac cggagcgatc attttacatg gaccacacca
     8461 tgttgcccaa aaatctacta gttgtacacc agattctact tttgaatcaa aatctgcatc
     8521 tgttactttt acgattgcca taaatgccaa tcctccttaa ttattattat gatgttcaaa
     8581 ttatatcaga taacatgctt catgtatttc tatttgctca atttttatgc ttatttcaaa
     8641 taacatgttt gaaatgtatt cttgttgctt tacattattt taactgcagc tatttaattt
     8701 atcaaattat aatttatttt agtgttgcaa cggtaacgcc aaatccacct tcgcttggca
     8761 taccacctct aaagtcacta acacttttat gctttttcaa atgttgttgt acaccttttt
     8821 gaagtgcacc tgtaccttta ccatgaatga tataaacttg ttcgtaatta cttaaaacgg
     8881 cttgatctaa atattgatct agttcaatta aagcatcctc ataacgatag cctcgtaagt
     8941 caagttcagt tttaattgtt tgacgatttt gacgtgtaac cattttcgtt ggcttaactt
     9001 tttctttttg ttttttctct aaatcttcaa taggtaactt cattttaata attcccattt
     9061 gaacaattgc ttcttcatca ttgacaattt ctaaaacttc acccttttga ccgtaagata
     9121 atacttttac ttcatcacca gcaacaattt tatcgtattt ttgcttttgt acattttgct
     9181 ttatagattt cgcttcataa tgatcatcta atcgtttctt cttatcaatc aattcatgtt
     9241 ctttaacatc tgcacctttt tgttcacgca attgtcttaa gtctttaata atatcgtcag
     9301 cttcttttgt tgcagcctta atcttctgat ttgctttttc tttcgcttcc tctattagag
     9361 acttttcata attttggaat tgttgatact gcttagataa atcatcatgc acttgctccg
     9421 cttcttttac aagacggtcc agttctaacc tctgtgtctc tacacgtttg taattacgct
     9481 ctaatgattc aatcatttca tttatttctt tttcatcagt accaatcatc gtcttagcct
     9541 tattaataat attcaaacta agacctaact ttttagaaat gtcaaaagca tttgaacgac
     9601 ccggcacacc cattaataac ttatacgttg gactcaaagt atctacatca aattctacac
     9661 tcgcattcat aacgccttct cgattataac tatatgcttt aagttcagga tagtgcgtcg
     9721 ttgccattac tagagaacca atttttctaa catgatctaa aatgctcatt gctaatgcag
     9781 caccttcact cggatctgta cctgcaccta attcatcaaa taaaactaaa ctatgtttgt
     9841 ctgcatgctt taaaatttca actatattcg tcatatgaga tgaaaaagtt gataatgatt
     9901 gttctattga ttgttcatct ccgatatcgc aatatacatt tttaaataca cttaactgac
     9961 taccatcaag tgtgggaatc aacaatcctg attgagccat aacaataatt aaacctaatg
    10021 tttttaatgt tacagtttta ccacctgtat tcggtcctgt aataattacc gtttcaatat
    10081 cttccataaa ttcgatggta ttagctacaa cagtctcacg atttaataat ggatggtatg
    10141 ctttaggtaa atatacagta cggtcctctt taaatatcgg ctttgttcct ttaatacttc
    10201 tactatatct cgcttttgcg attaaaaaat ctaactgacc catgacttgt tctgccacaa
    10261 gtagtgcatc tttgtccgca gccacataac cagttagttg cgttaaaatg cgttcttttt
    10321 caattgcttc gtcatgacgt aatcgactaa tttgattatt catttcaaca actgatgatg
    10381 gctcaatata caatgtttgt cctgaagcag attgatcatg tacaatccca ttaaaatctt
    10441 gtcgatattc agctttgaca ggtataacgt ttctttcatt cctaactgtt acaatagcat
    10501 ctgataattt tttctgattt gcttggcttt taacaatacg gtccaaattt tgtctaatac
    10561 gttgattcgt gctagaaatt ttacttctaa tcccttgcaa ttcataactc gcattatcat
    10621 ataaatcata cgtatcgcat gtttcattta tttgttgaaa aagatcagtt aacacaggta
    10681 attgattcat cttgtcatct aatattgggt atttaacacc ttcatcttct tcaaccaatt
    10741 gattataaaa tgtcttgaat tgattttgta cttgaattaa tctttttatc aagttaagct
    10801 ctgatacatt taaaacgccg ccaatatcag cgcgatgaat gaatgctgat actttagata
    10861 agccactcaa gcttggtaaa cgatgcttat tatagatttg agcaatctca tccgtttctt
    10921 ccatttgaaa aacaaccgtt tcaaaattag tagctggcat catttgattg accttttcca
    10981 agcctaagtc actaatagtt tcattggcaa cgagtgattt tattttttca aattctaaga
    11041 cgtctaatgt tttttgtctc ataaaatccc tctatttctt aagttttgta ttattttcaa
    11101 taaagtcttt aaaagcttca cgcgataatg cgttaatcac tctatctttt gttacaaatc
    11161 ctttttgcgc agttgctacg ccataattca taaaatctaa atgatttgta tgatgcgcat
    11221 cagtgttaat agttaatttc acatttggat atttacgaac gatatcagcg ctcagatcca
    11281 gtcgatgtgg attggcatta atttctaata ctgtattcgt ttcttcagct aatgccatta
    11341 attgttcaat attcggttta taaccatctc ttctacctat aatacgccct gttggatgcg
    11401 ctatatgtcg cacgtatgga ttgcgacatg cattagctaa tcgttccata atttgttctt
    11461 ctgattggtt aaagctttga tgaatagctc caattacata atcaagttgt gctaaaattt
    11521 catcatcata atccagcgag ccatcaggta atatatccat ttctgtacct gaataaatat
    11581 caatttcact atattcttta tctaaagcct taatttcttc gttttgtctt aaaagtcttt
    11641 ctacttgtaa gccattagca acacgtaaac tttgtgaatg atcagtaatt accatgaatt
    11701 tataaccttt tgcgatattt gcttctacca tgtctcgaat agaaaacgca ccatcactat
    11761 acgttgtatg catatgaata tcaccattaa tatcatctat tgtaatgata ttacttagat
    11821 ctttatcaaa ttcgctacca tcttctcgca tagcaggtgg tataaaattc acattaaaat
    11881 gttcatatat cttggcttca ctatcatatt gaattaatgt accatcagct tgttcaattc
    11941 catattcact tactttttca tcacgtgctt tagcaagttg tcgaattctt atattatgtt
    12001 cttttgaccc agtaaaatgc tgcaatgtat gataaaaagc acttggttca attaatcgaa
    12061 aatcgacacc aatcgtttca tcatcatacg ctaattctaa tgaaactttt gtgttcccca
    12121 ctgcaacttc ttttacttta ttgggaatat ttaataattg ctgctgcact gcttttgggt
    12181 tatcggtact tattatgaaa tctaaatctt tgctcatttc tttaaaacga cggaagcttc
    12241 ctgcagatga atattgatcg
//
LOCUS       DAMQYT010000061        12102 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMQYT010000061 DAMQYT010000000
VERSION     DAMQYT010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12102
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.061"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..435)
                     /locus_tag="PGA33_001299"
     CDS             complement(<1..435)
                     /locus_tag="PGA33_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HDG6473118.1"
                     /translation="MKFTALAKATLALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAF"
     gene            complement(801..1499)
                     /locus_tag="PGA33_001300"
     CDS             complement(801..1499)
                     /locus_tag="PGA33_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HDG6473119.1"
                     /translation="MKFTVIAKAIFILGILTTSVMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSIDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGSADKGRIVINMKDEKKHEIDLSEKLSFERMFDV
                     MDSKQIKNIEVNLN"
     gene            complement(1843..2538)
                     /locus_tag="PGA33_001301"
     CDS             complement(1843..2538)
                     /locus_tag="PGA33_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HDG6473120.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVQAKEKQERVQHLYDIK
                     DLHRYYSSESFEFSNISGKVENYNGSNVVRFNQENQNHQLFLLGKDKEKYKEGIEGKD
                     VFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFSINKEEV
                     SLKELDFKIRQHLVKNYGLYKGTTKYGKITINLKDGEKQEIDLGDKLQFERMGDVLNS
                     KDINKIEVTLKQI"
     gene            complement(2959..3654)
                     /locus_tag="PGA33_001302"
     CDS             complement(2959..3654)
                     /locus_tag="PGA33_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL6"
                     /protein_id="HDG6473121.1"
                     /translation="MKLKTLAKATLVLGLLATGVITTESQTVKAAESTQGQHNYKSLK
                     YYYSKPSIELKNLDGLYRQKVTDKGVYVWKDRKDYFVGLLGKDIEKYPQGEHDKQDAF
                     LVIEEETVNGRQYSIGGLSKTNSKEFSKEVDVKVTRKIDESSEKSKDSKFKITKEEIS
                     LKELDFKLRKKLMEEEKLYGAVNNRKGKIVVKMEDDKFYTFELTKKLQPHRMGDTIDG
                     TKIKEINVELEYK"
     gene            complement(4100..4804)
                     /locus_tag="PGA33_001303"
     CDS             complement(4100..4804)
                     /locus_tag="PGA33_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HDG6473122.1"
                     /translation="MKMTAIAKASLALGILATGTITSLHQTVNASEHKAKYENVTKDI
                     FDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKNRKFTRVQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
     gene            complement(5168..6094)
                     /locus_tag="PGA33_001304"
     CDS             complement(5168..6094)
                     /locus_tag="PGA33_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HDG6473123.1"
                     /translation="MKITTIAKTSLALGLLTTGVITTTTQAANATTPSSTKVEAPQST
                     PPSTKIEAPQSKPNATTPPSTKVEAPQQTANATTPPSTKVTTPPSTNTPQPMQSTKSD
                     TPQSPTTKQVPTEINPKFKDLRAYYTKPSLEFKNEIGIILKKWTTIRFMNVVPDYFIY
                     KIALVGKDDKKYGEGVHRNVDVFVVLEENNYNLEKYSVGGITKSNSKKVDHKAGVRIT
                     KEDNKGTISHDVSEFKITKEQISLKELDFKLRKQLIEKNNLYGNVGSGKIVIKMKNGG
                     KYTFELHKKLQENRMADVIDGTNIDNIEVNIK"
     gene            complement(6203..6340)
                     /locus_tag="PGA33_001305"
     CDS             complement(6203..6340)
                     /locus_tag="PGA33_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473124.1"
                     /translation="MFVKVAFLCLKSDETSNVPSVESHQNHFYLTNIMDFLIYLTMIQ
                     I"
     gene            complement(6459..7517)
                     /locus_tag="PGA33_001306"
     CDS             complement(6459..7517)
                     /locus_tag="PGA33_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HDG6473125.1"
                     /translation="MKMRTIAKTSLALGLLTTGAITVTTQSVKAEKIQSTKVDKVPTL
                     KAERLAMINITAGANSATTQAANTRQERTPKLEKAPNTNEEKTSASKIEKISQPKQEE
                     QKTLNISATPAPKQEQSQTTTESTTPKTKVTTPPSTNTPQPMQSTKSDTPQSPTIKQA
                     QTDMTPKYEDLRAYYTKPSFEFEKQFGFMLKPWTTVRFMNVIPNRFIYKIALVGKDEK
                     KYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVNHKVELSITKKDNQGMISR
                     DVSEYMITKEEISLKELDFKLRKQLIEKHNLYGNMGSGTIVIKMKNGGKYTFELHKKL
                     QEHRMAGTNIDNIEVNIK"
     gene            complement(7808..8503)
                     /locus_tag="PGA33_001307"
     CDS             complement(7808..8503)
                     /locus_tag="PGA33_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HDG6473126.1"
                     /translation="MKMKNIAKISLLLGILATGVNTTTEKPVHAEKKPIVISENSKKL
                     KAYYNQPSIEYKNVTGYISFIQPSIKFMNIIDGNSVNNIALIGKDKQHYHTGVHRNLN
                     IFYVNEDKRFEGAKYSIGGITSANDKAVDLIAEARVIKEDHTGEYDYDFFPFKIDKEA
                     MSLKEIDFKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINV
                     TDIDRIEIKVIKA"
     gene            complement(8789..9469)
                     /locus_tag="PGA33_001308"
     CDS             complement(8789..9469)
                     /locus_tag="PGA33_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HDG6473127.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKAEVKQQSESELKH
                     YYNKPILERKNVTGFKYTDEGKHYLEVTVGQQHSRITLLGSDKDKFKDGENSNIDVFI
                     LREGDSRQATNYSIGGVTKSNSVQYIDYINTPILEIKKDNEDVLKDFYYISKEDISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMNDGTTHTIDLSQKLEKERMGESIDGTKI
                     NKILVEMK"
     gene            9941..10786
                     /locus_tag="PGA33_001309"
     CDS             9941..10786
                     /locus_tag="PGA33_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDG6473128.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVPVRQLDYFNQESMVEAFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYAARLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNDQPQTLQSF
                     LQENI"
     gene            10805..11164
                     /locus_tag="PGA33_001310"
     CDS             10805..11164
                     /locus_tag="PGA33_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HDG6473129.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINLLLKNQGVYNGLIGVLLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            11688..11840
                     /locus_tag="PGA33_001311"
     CDS             11688..11840
                     /locus_tag="PGA33_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473130.1"
                     /translation="MVPEEKGSITLSKEAAIIFAIAKFKPFKNRIKNNPQKTNPFLKL
                     HENKKS"
BASE COUNT         3648 a         2089 c         1644 g         4721 t
ORIGIN      
        1 aaaagcatct tcaccctttt cttttgaaat atttaatatt ggtgcactga caactccaga
       61 tgttttatta ttctgtatta caccaccaac agtataaaat atatcgtgct tatctctttt
      121 ttcttgaaca aagaaaacat ctaacccctc ataactacgc tgttgaaatt tacttttatc
      181 atttccaggc agtaaaactt gaatcttaat acctctaaac ttaaaacgta agttgttttt
      241 accatgtttc aaagcactaa tatttttcat ttccatagtc tttccagtgt agtatcggta
      301 taatgcttcc ttgtcatgtt tatttactga cttttgattt tgttttgcat gacctgaatg
      361 aacttctgtt gttaaagttc ctgttgttaa aattcctaaa gctaatgtcg cttttgctaa
      421 tgctgtaaat ttcatattta tttgctccaa tcttaatata ttggattgtt ttcattacgt
      481 aatttgaatc atacattttt attataacgt gcatcgtttc atacgataat taacctcgat
      541 ttaaatatac ttgaattatt cttaatgaaa aacaaatata ttttaatgtt caggttaaat
      601 gactttcatt atttcattta gttgttttac cggattatcg tttactacaa ctgatgatgt
      661 tactctgttc tgctctttga attacaaaaa caacttaaca acagtaatgt atttaacaca
      721 gttccttata taaggacaca aatgccaatt cacattttgt cgttaagccg cttcttaagc
      781 tattatgcta ttaactcgaa ctaattcaaa ttcacttcaa tatttttaat ttgcttacta
      841 tccattacat caaacatacg ttcaaaactt aatttttcac ttaaatcaat ttcatgcttc
      901 ttttcgtctt tcatattgat aacaattcta cctttatcag ctgacccttc atacagtttg
      961 tattttttaa tcaacagttt tcttatttta aaatcaagtt ccttcaatga cacttcttcc
     1021 ttttgaataa agaaaaattc atcaatagaa aaaccgtctt tatcgtctat ttttttaact
     1081 agtaagttcg gcgttcttag agactcaaat attgatttta cgtttttctt tgttacacca
     1141 ctaacactaa atattcttcc atctaaatct actaattctg gtaccgcaaa gacatctaaa
     1201 ccatgtgttt tttctttgta tttattttca tcttttccca ataaaaacac ttggaacttt
     1261 tgattttgtt ggttaaaaag caaaacgtta gagtcataat aaccttgact ttggccacta
     1321 acatttgtta actcataact aggtcctgaa tagtattgat gtaacttgtt tgtatcatat
     1381 aaacggttca ttttttcata ctttcctttt gcattaaccg attgattttc tgttatcatt
     1441 acacttgttg ttaatattcc taatataaat atcgctttag ctatcactgt aaatttcata
     1501 ttcatgtgct ccaatcttaa tatattggat tgcttttatt acgtaatttg aataatacac
     1561 ctttattata aagggcattg agacagactg acattaacac tcgtttaatt aaacttaaat
     1621 cattgttaat gaagagctaa ttaatattca atgggcatca ctttcaagag caatgtaaat
     1681 ttttagtatg ctcactcata acctgcgtta taaaacgtaa aaaaaagcct aacgacaagg
     1741 ttgttttaaa tacgctcctt aatgtaaggt gtacgtatct attcaacatt tcatcgttaa
     1801 gctgcttcaa atttattact ttagagtcat tgattactta ctttaaattt gtttcaaagt
     1861 cacttcaatc ttattaatat ccttactatt caacacatca cccatgcgct cgaattgcaa
     1921 tttatcacct aaatcaattt cttgcttttc tccatctttc aaattgatag tgatcttacc
     1981 gtatttagtc gtacctttat ataaaccata atttttaact aaatgttgtc taattttgaa
     2041 atcaagttct ttcagtgaaa cttcttcttt attaattgaa aatgagtcaa ttgatgcatc
     2101 taaatttccg ccatacactt tattaacaaa taaatgtgta ttagtttcag aagatttgtt
     2161 atttttctta gtcacaccac caacagtaga taatctaccg tttggatcaa ttaattcttt
     2221 taccacaaag acatctttgc cttcaatgcc ttctttatat ttctctttat ctttacctaa
     2281 taagaataat tggtgatttt gattttcttg gttaaagcgt acaacgttag aaccgttata
     2341 attttcaacc ttaccactaa tattactgaa ttcaaaactt tctgatgagt agtatcgatg
     2401 taagtcttta atatcatata aatgttgtac tctctcttgc ttttcttttg cttgaactgc
     2461 ttggccttct gatgtaatca caccagtagt taataagcct aatgccaatg ttgctttagc
     2521 taacgttttt aatttcacag tactattctc ccaatctatt tataaatttg tcttaatata
     2581 tttttatatg attaattcaa ttacgtaata cgaacaatct actatcatta tataagtgag
     2641 atactgcgct atgaattaac ctctttttaa ctatttttga accaacgtta agttagttaa
     2701 tgaaatccta acagtattaa ttatttttta taacacaata ctaatttttt aactattcac
     2761 actttccctc aatttaatta tgttttccac attgtttcat gtcacgaaaa ggacaacgcg
     2821 cgactataag tatcaaccgt ttttccaaaa acttgatcat aaacccgctc cttttttcat
     2881 cataacaaaa taaaaaagaa gctaagcaat atgttatcgc ttaacttccc tacaattact
     2941 agtctgcttg tccaaagatt atttatattc tagctcaaca ttaatttctt tgattttggt
     3001 accatctatc gtgtcaccca tgcgatgcgg ttgtagtttt tttgtaagtt cgaaagtata
     3061 aaacttatca tcttccattt taactacaat tttacctttt ctattattaa cagcaccata
     3121 taatttttct tcttccatca atttttttct taatttaaag tccaactctt ttaacgagat
     3181 ttcttcttta gtaattttaa atttactatc tttagacttt tccgatgatt catcaatttt
     3241 tcttgttact ttaacatcga cttctttact aaattcttta ctatttgtct tacttaaacc
     3301 accaattgaa tattgtcttc cattaacagt ttcctcctcg atgactaaaa atgcatcttg
     3361 cttatcatgc tcaccttgag ggtatttttc aatatcttta ccaagcaagc caacaaaata
     3421 atcttttcga tccttccaaa catatactcc tttatctgtc actttctgtc tatacaaacc
     3481 atcaagattt tttaactcta tacttggctt gctatagtag tattttaacg atttataatt
     3541 gtgttgacct tgagttgatt ctgccgcttt tactgtttga ctttctgttg ttattacacc
     3601 agtagctaac aatcccaata ctaatgttgc tttagctaac gtttttaatt tcatagtact
     3661 attctcccaa tctatttata aattttgtct taatatattt ttatatgatt atttcaatta
     3721 cgtaatacga acaatctact atcattatat aagcgtaata ctaagctatg aattaacctc
     3781 tttttaacta tttttgaacc aatgttaagc taattaatgg aatcctaaca gcgttaatct
     3841 attttttaaa cttaacgcag cttttttaac tattcacact ttccctcaat ttaactatgt
     3901 tttccacatt gtttcatgtc acgaaaagga caacgcgcga ctataagtat caactatttc
     3961 cacaagtttt attggtgttt ttattattca tcgatacgct tcattttcat ctctccaaca
     4021 caaaaaagaa gctaagcaac ttatgttgcc taactcctct atactatcca tattttacta
     4081 ttatccatat ttcattgaat tatctaatgt tggcttctat tttttcaata tttctaccgt
     4141 caatgacgtc actcatgcga tttgtttgta attttttatt aagttcaaac gtataatagc
     4201 cgccatcttt cattatcact tttatcttac tatctttagg aaacttttta tacagatcaa
     4261 aattttgaat taaatactgt ctcaatttaa agtcgagttc tttaagtgaa atctcttctt
     4321 tataaatgta gtgtactcta ccgtacgtag caataccgtc accttcatct ctcttgattt
     4381 gaaatcttgg tgcgtttata taatcataat aagcgtcttg atttttctta gtgacaccac
     4441 catatgaaaa cactgtgcca ttacggtttt ccgcttcttt aacaacaaat atgtctaatc
     4501 ccggattttt acgtgcttta aatctttcaa tatctttacc aaatatctgt actcttgtga
     4561 attttctatt tttatcaaag ataaggtaat gcttgccacc tttgctataa cgataaccag
     4621 taacattttt aagttcctta cttgcgccac tatagtaatc tcttaagtca aagatatctt
     4681 ttgtcacatt ttcatatttt gctttatgtt cactcgcatt tacagtttga tgcaatgacg
     4741 ttattgttcc tgttgctaaa atacctaatg ctaaacttgc tttcgcaatt gctgtcattt
     4801 tcatagttgt atgctccatt cgtaattatt agatttgttc gcttacgtct attgaatcat
     4861 acagctttat tatagttagc gtatttgacc tttcacatta aaccatgttt aataatcatt
     4921 gaatcattat taagtaaatt aaggaatcta taatgttcgt taaataaaac tgatcccgtt
     4981 gtgcttcaca cccgatagat agggatttac agataaattc aggtctcttc cacgtcatat
     5041 ttggacccat cgaaaattcg ggttctcaaa tcatcgaaca taacaaaaga agctaagcaa
     5101 catgtaggcc gttgtcactt aacttcttgt ttttccgatg acagcttcta tttagagaat
     5161 gtcatgatta ttttatattc acttcaatgt tatcaatatt agtgccatct atgacatctg
     5221 ccatgcgatt ttcttgtaat tttttgtgca attcaaacgt gtactttcca ccgtttttca
     5281 ttttaataac aattttacct gaaccaacgt taccgtacag attatttttt tcaataagtt
     5341 gttttctcaa tttaaaatca agttctttca aggaaatctg ttctttagta atcttgaatt
     5401 ctgaaacatc atgagagatt gtacctttat tatcttcctt agtaattctt actcctgctt
     5461 tgtgatcaac ttttttacta ttactctttg tgataccacc gacagaatat ttttccagat
     5521 tgtaattatt ttcttctaaa acgacaaata catcgacatt cctatgtact ccttcaccat
     5581 attttttatc atctttacca actaaagcaa ttttatatat gaaataatct gggacaacat
     5641 tcataaatct tattgtcgtc cattttttta aaataatacc aatctcattt ttaaattcta
     5701 aacttggttt cgtataatac gctcttaaat ctttaaattt aggatttatt tctgttggta
     5761 cttgttttgt ggttggcgat tgtggtgtgt ctgatttagt agattgcatt ggttgtggcg
     5821 tgtttgttga tggaggtgtt gtcactttag ttgaaggcgg tgttgtcgca tttgctgttt
     5881 gttgcggtgc ttctacttta gttgagggcg gtgttgtcgc gtttggtttt gattgcggtg
     5941 cttctatttt agttgagggc ggtgttgatt gtggtgcttc cactttagtg gaagatggtg
     6001 ttgtcgcgtt tgctgcttgc gttgtcgttg tgattacacc tgttgttaaa aggcctagtg
     6061 ctaaacttgt tttagcaatc gttgttattt tcatagttgt atgctccatt cgtaattatt
     6121 agatttgttc gattacattc attgaatcat acagctttat tatagatggc gtattgctcc
     6181 attcacatta aaccttgttt aactatattt gaatcatcgt taagtaaatt aagaaatcca
     6241 taatgttcgt taaataaaaa tgattttgat gtgattcaac acttggcaca tttgaagttt
     6301 cgtcactttt aagacataga aatgccactt ttacaaacaa atgaatattc gtctttttac
     6361 atcattacgc ataataaaag aagctaagca acatgtaaac cgttgtcact taacttcttg
     6421 tttttccgat gacagcttct atttagagaa tgtcatgatt attttatatt cacttcaatg
     6481 ttatcaatat tagtgcctgc catacgatgc tcttgcagtt ttttgtgtaa ttcaaacgta
     6541 tatttcccac cgtttttcat tttaataacg attgttcctg aacccatgtt accgtaaaga
     6601 ttatgttttt caataagttg ttttctcaat ttaaaatcaa gctctttcaa ggaaatctct
     6661 tccttagtaa tcatgtattc tgaaacatcg cgtgaaatca taccttgatt atctttttta
     6721 gtaatgctta attctacttt gtgattaact tttttactat tagtcttcgt gatgccaccg
     6781 acagaatatt ttttcaattg atatttattg tcttctaaaa cgataaatac atcgatatta
     6841 tcgtaaggtc catctttata ttttttctca tcttttccaa ctaaagctat tttatagatg
     6901 aacctatttg gaataacatt cataaaccta accgtcgtcc atggtttgag cataaatcca
     6961 aactgctttt caaattcaaa actcggtttt gtataatacg ctcttaaatc ttcatattta
     7021 ggagtcatat ctgtttgtgc ttgttttatg gttggagatt gtggtgtgtc tgatttagta
     7081 gattgcattg gttgtggcgt gtttgttgat ggaggtgttg tcactttagt tttcggcgtt
     7141 gtggattcgg ttgtcgtttg tgattgttct tgtttaggcg ctggcgttgc tgatatatta
     7201 agcgttttct gctcttcttg tttaggttgt gatatttttt ctattttgga agctgaggtt
     7261 ttttcctcat tagtatttgg tgccttttcg agtttaggcg tgcgttcttg tcttgtgtta
     7321 gctgcttgtg ttgtcgctga atttgcacct gctgttatgt ttatcattgc taatcgctct
     7381 gctttaagcg ttggtacttt gtcaacttta gttgattgta ttttttctgc tttgaccgat
     7441 tgcgtcgtta ctgtaattgc gcctgttgtt aaaagcccta gtgctaaact ggttttagca
     7501 attgttctca ttttcataat tgtatgctcc aatctatatt atattcgatt gtctttttac
     7561 gtaatttgaa tcatacaaca tcattataga tggcgttcta agataatcac attaaacccc
     7621 ttttaacaat tattgaagta ttattaagta atttaagcaa aaaataatga gtgagtatga
     7681 gattaatata gcgtttctat gtgcctttga aataattttt aagcattaaa aagaagttaa
     7741 gcaacgtttg atcgtcactt aacctctcta tttcaatttc aacttatttc gtcatcaagt
     7801 atatgtgtta tgcttttata actttgattt caattctatc aatatctgtg acattgataa
     7861 catcggacat acggtcttct tgtaactttt tatccaattc aaatgtatac tttccatagt
     7921 atttcttttt gactgtaatt tttcctgtac tcatttcacc gtaaagacca taattatcaa
     7981 taaggtattt tcttaattta aaatcaatct ctttcaatga catcgcttct ttatctattt
     8041 taaatgggaa aaagtcataa tcatattcac cagtatgatc ttctttaata actcttgctt
     8101 ctgctattag gtcgacagct ttatcgtttg cactcgtgat acccccaata gagtactttg
     8161 caccttcaaa tctcttatcc tcattaacgt aaaatatatt aagattacga tgtacacccg
     8221 tatgataatg ttgcttatct ttgccaatta aagcaatatt attaacagaa ttaccatcta
     8281 tgatattcat aaatttaata cttggttgaa tgaaactgat ataacctgtc acatttttat
     8341 attcaatact aggttgatta taataagctt ttaatttttt gctattttca cttattacaa
     8401 taggtttctt ttcggcatga actggttttt ccgttgtagt gtttacacct gttgctaata
     8461 ttcctaataa caaacttatt tttgcaatat ttttcatttt catagttgta tgctccaatc
     8521 tattataatt agattgtttt attacgtaat ttgaatcata cacccatatt ataggagctg
     8581 tattcggata ttcacattaa cctgttttta actattcata aaatatgatt aagctattta
     8641 agcaaaagat aatgcgtggt gcgactgttt gtgtgagttt tgtaactttt cccctcaaaa
     8701 aaagaagcta agcaacagta cgtcgcttaa cttccatata tttccataac aagcactatt
     8761 caacatagcg ctttgttgtt agaaagtatt atttcatttc tactagaatt ttattaatct
     8821 tagtgccgtc gattgactca cccatacgtt ctttttcaag tttttgactt aaatcgattg
     8881 tatgtgttgt gccatcattc attgtaattg taatttgacc ttgtttaaga ccatttgaat
     8941 acaagccgtg ttgtttaatc gcacgttctc ttaatctata atcaagttct tttaatgaga
     9001 tgtcttcttt tgaaatgtag taaaaatctt taagtacatc ttcattatct ttcttgattt
     9061 ctaaaattgg cgtattgata taatcaatat actgcacact atttgatttt gtaacgccac
     9121 caattgagta atttgttgct tgtctactgt caccttctct aaggataaac acatctatat
     9181 ttgagttttc tccgtcttta aatttatctt tatcagatcc aagtaaagtg attcgagaat
     9241 gctgttgccc tactgtgact tctaaatagt gtttaccctc atcagtatat ttaaatccag
     9301 tcacattttt acgctctaaa attggtttat tataatagtg ttttaactct gattcacttt
     9361 gttgtttaac ttctgctttc gcttgtactg attgtacatt cgatgtaatt acacctgttg
     9421 ctaacattcc caatgctaaa cttgcttttg ctatcgcttt aaatttcata atttttagcc
     9481 ccaatctatt taaatttgat ttttcaatta cgtaatatga acaatctacc tacattatat
     9541 tgaggcgcta tcccctttca aattaaacct catttaacta aacttgaatc attgttaagg
     9601 taattaataa aatgctaact gttattaatc aattcttatc cgtcgagact cttttttata
     9661 caaatcacac tttataccat ttttatttta aaaactgtaa aactttcaga cacaaaacag
     9721 acatcatatt gtcgtagtac ctgattaaaa ttttcaatga tattagtgat acacatcaac
     9781 taaaacgcgt cattacggca ttttcaacat taataatatt tacgtattta ctatacacgt
     9841 cgacaataaa tacagattag aattttttat aaaatcaaat gttttgaaaa ccatcaaata
     9901 agtattatgt tatgtatata aagattagga gatgaagtga atgaatatta tgttaacagg
     9961 tgctacaggt catttaggca cacatattac aaatcaagcc attgcaaatc acatagatca
    10021 ttttcacatt ggtgttagaa atgttgagaa agttccagaa gattggcgcg gaaaagttcc
    10081 tgttcgacaa ttagattatt ttaatcaaga aagcatggta gaagcattta aaggaatgga
    10141 taccgttgtg tttattccaa gtattatcca tccatcattc aaacgtattc ctgaagtgga
    10201 aaatttagta tatgcggcaa agcagagcgg cgttgctcat atcattttca taggttatta
    10261 cgcagatcag cataataatc cattccatat gagtccttat tttggttatg cagcacgtct
    10321 attggcaaca agtggcattg actatacgta tgtaagaatg gcaatgtaca tggatccact
    10381 taaaccatat ttaccagaat tgatgaatat gcataaactg atttatccag ctggcgatgg
    10441 tcgtattaat tatattacta gaaatgatat tgctagaggt gtcattgcta ttattaaaaa
    10501 tccagatact tggggcaaac gctacttatt atcaggctac agttatgata tgaaagaact
    10561 tgctgcaatt ttatctgagg catcaggcac agaaattaaa tatgagcccg tttcattaga
    10621 gacatttgca gaaatgtatg atgaacctaa aggctttggt gcattattgg catcaatgta
    10681 cgacgcagga gcaagaggac tattagacca agaatccaat gatttccaac aattagtcaa
    10741 tgatcaacca cagacactgc aatcattttt acaagaaaat atttaataat aaaggagcgt
    10801 tatagtgaat atcatctcaa caattttaat catatttgtg gcattagagt ttttctatat
    10861 tatgtacctt gaaacgattg ctacaacttc caaaaagact agcgagacat ttaatataag
    10921 cgtcgataaa ttgaaagaca aaaatattaa cctacttttg aagaaccaag gcgtatataa
    10981 cggtttaatc ggagttttgc taatatacgg tttgtttatc agcagtaatc caaaagaaat
    11041 atgcgcagct attttagtgt atatcattgg cgttgctatt tatggtggcc tttcaagcaa
    11101 tattagtatc tttttcaaac aaggcacatt gccagtattg gcactcatat caatgctttg
    11161 gtaagtattg gtgtttgggg gggtggagat gtagtcggag gtttggagga tttgagcgaa
    11221 ttgtgtgtgg actttagact cagagtattt catcctaatt atttcaagca gaggtgacag
    11281 tagcgttgcc tctgtttcct tatagaaaaa ttatttaatg aagaaaaccc atatctgatt
    11341 taattttcag ctgataaata ctccatatat tagaatggct actttatcta ttgcatcaat
    11401 cctttaaaac aaaaaaccca tgatttcgaa attcccgtat gatggggttc ctactctcat
    11461 ggatcagtta aataaatatt atcactatca gtttattatt tcaatattat taacaatata
    11521 tgtagtcgta aaaggaaaga ggacatgaga acttcggtgt tgattggcat tacataacgc
    11581 ttccaaacat attatttggt aacaataaga aactatttac acaatatatt ttgtatagta
    11641 aaattatttt ataatattta aatccaattg cacaaggagt gattatcatg gtaccagaag
    11701 aaaaaggttc tattactttg tcaaaagaag cagctatcat atttgcaatc gcaaaattca
    11761 aaccatttaa gaacagaatt aaaaataacc cacaaaaaac aaatccattt cttaaattac
    11821 atgaaaacaa aaaatcttaa tcactttatt tatagcattt ctaatctcag aaatgctata
    11881 aaggacaacg ccctctttca taactatata agtcaagaaa ccaagacatt tcaaacatct
    11941 ttaacccctt caatagcaca ttccctaaaa cataaagcaa taacaatgaa cgatcaaaat
    12001 tccttacgta cttatgatgt gtcagtgaat cgtctacact taattgaaca aattctatga
    12061 gaatagatat tgttaaatta agaaagtagt caattttatt at
//
LOCUS       DAMQYT010000062        12036 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMQYT010000062 DAMQYT010000000
VERSION     DAMQYT010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12036
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.062"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            143..343
                     /locus_tag="PGA33_001312"
     CDS             143..343
                     /locus_tag="PGA33_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDG6473131.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(470..1039)
                     /locus_tag="PGA33_001313"
     CDS             complement(470..1039)
                     /locus_tag="PGA33_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDG6473132.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGVTVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVIGILSLIIFLSVYLANKFL"
     gene            complement(1299..1694)
                     /locus_tag="PGA33_001314"
     CDS             complement(1299..1694)
                     /locus_tag="PGA33_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HDG6473133.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIVVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            complement(1762..2115)
                     /locus_tag="PGA33_001315"
     CDS             complement(1762..2115)
                     /locus_tag="PGA33_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HDG6473134.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            complement(2568..3407)
                     /gene="noc"
                     /locus_tag="PGA33_001316"
     CDS             complement(2568..3407)
                     /gene="noc"
                     /locus_tag="PGA33_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDG6473135.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(3450..4169)
                     /gene="rsmG"
                     /locus_tag="PGA33_001317"
     CDS             complement(3450..4169)
                     /gene="rsmG"
                     /locus_tag="PGA33_001317"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDG6473136.1"
                     /translation="MTVEWLAEQLKEHNIQLTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(4169..6046)
                     /gene="mnmG"
                     /locus_tag="PGA33_001318"
     CDS             complement(4169..6046)
                     /gene="mnmG"
                     /locus_tag="PGA33_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDG6473137.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILELLEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(6113..7492)
                     /gene="mnmE"
                     /locus_tag="PGA33_001319"
     CDS             complement(6113..7492)
                     /gene="mnmE"
                     /locus_tag="PGA33_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDG6473138.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVIEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(7631..7984)
                     /gene="rnpA"
                     /locus_tag="PGA33_001320"
     CDS             complement(7631..7984)
                     /gene="rnpA"
                     /locus_tag="PGA33_001320"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDG6473139.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(8105..8242)
                     /gene="rpmH"
                     /locus_tag="PGA33_001321"
     CDS             complement(8105..8242)
                     /gene="rpmH"
                     /locus_tag="PGA33_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDG6473140.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            8973..10334
                     /gene="dnaA"
                     /locus_tag="PGA33_001322"
     CDS             8973..10334
                     /gene="dnaA"
                     /locus_tag="PGA33_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDG6473141.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            10612..11745
                     /gene="dnaN"
                     /locus_tag="PGA33_001323"
     CDS             10612..11745
                     /gene="dnaN"
                     /locus_tag="PGA33_001323"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDG6473142.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
BASE COUNT         3945 a         2236 c         1753 g         4102 t
ORIGIN      
        1 ttatgccaat atgagcgtat agttggtctt acgtaataaa agctcgtgaa ttaaattgta
       61 gtgtatttgt ttagaatatc ctctttttta gttatgaatt tgttacaaat attaagtgca
      121 aaagcacacg gaggttttct atatgaataa cggtacagta aaatggttta acgcagaaaa
      181 aggttttggt ttcatcgaac aagaaaatgg cggagacgta ttcgtacatt tctcaggtat
      241 cgctagcgat ggctacaaaa ctttagaaga aggtcaaaaa gttactttcg aaatcactga
      301 aggtcaacgt ggagaccaag cagttaacgt acaaactgtt taatcttaca acataaaacg
      361 actcattata aatgaatcgc tttgattacc aacaaggttc taaatcttgt tggtattttt
      421 ttatgctatt ttggataata taaataatat gagacgatac cacgatgatt tataaaaact
      481 tatttgccaa atacacacta aggaaaatta tgaggctgag tataccaatt actgatataa
      541 tagaaagcgc atttgtcatc gtatttctag ctttagtcgt ttgtacttca ctaggatctt
      601 ttccattcat aaaagcaaca attcgatcgt agttgtccat tttatgcact ttttttaaat
      661 cttctatttt catagatgca taaaaaccta ttcccaaaat gattgccacc gttacacccc
      721 aagtccaatt ccaataaaca actaaaggtc ccattaaaat tgcagctaat gtcatgaaaa
      781 taagcatcac atatgtccat aagttaagac ttcgttgcgc tttaatatca ggtacaaatg
      841 gaatggtccc ttttactaaa tcatctaaag ttacattgaa caattcacac atcattaata
      901 agttatgtat gtctggtaag cttttgtcat tttcccaatt agaaatactc tgcctagata
      961 catataactt ttcagcaaga tattcttgtg aataaccatc tcgttcccta tactttttaa
     1021 tttgtttact cagattcaat tacatacgcc tccagtcaat tagtacaatc attacaatca
     1081 gactatcaga taaagttaaa tcaagtcacc tttatgattg actaaatgtt ttgacatacg
     1141 acaacatcgg ggttcgaaga ctcccttaag acagacctgt atgattacgc gcttgtaatt
     1201 ttctcaatgc caacttgaat ttcacttatt aatgatgcaa cttttccatg ctattcaatg
     1261 cttgcaaagt tagtcctagt taagagatta cgttcacact atttactatt cgattttcca
     1321 gctttccaac tatgcttttc taatcttttt tgtgctataa attctaccgc ttcaaaaata
     1381 catactgcac tgatgaacca gcttaggaaa ccaacaacaa tgctaatcaa ccacatgtgc
     1441 gtcatatgta acatcatcca tgtaactaaa atacaaacta atacccctgt cataccaaac
     1501 actgcgccac gacttacatg cacagcgact ttatcaccat attgcatacc tgtaataaac
     1561 atagacacta aaaatactgc cggaaacgtt gcaaatatac cgccaaattc tttccaaggt
     1621 agtgttacag atacaatata acttaataat actgcaaatc cccccacaaa aaatttcatt
     1681 aatgttaatt tcatgatgtt ctgcctccaa taatttatta cttaataact gaatacaact
     1741 aattatcacg cattttccaa actataaatg tctaatactg aatattgcga gggaaatgac
     1801 aaaccaacaa acaactgtga atattgcgcc tttccgataa cctttatgct taatgtagag
     1861 agatgtcaaa aatacagtta atatacaaga cagaataccg acaattgctc cagtactaag
     1921 attcatcgac atctccacta attgcgtacc acgatgatct aaagctaatg cgataatagc
     1981 cgcaagaaag actgccggca tagtagctat aatacctcct aacttaccac ctaccttatc
     2041 agcaataatt gaagctaatg caacagcgat accaccaatg acaaaatgaa gaattgcact
     2101 tccaattgaa aacatcttca acgtaccttt ctataattaa ttcaatacaa aacgtatcat
     2161 tcacacaaag tgtaaaactt aaattttcta ctgtaaaggc aactcaagcg atgttcataa
     2221 agctttcatg atactttcat aaactagcaa taaagggttc aaatgaaagt atatccagct
     2281 caaattaggg agtgggacag aaatgatatt ttcgcaaaat ttatttcgtc gtcccacccc
     2341 agcaaggttg actagaattg aaaaaagctt gttacaagcg cattttcgtt cagtcaacta
     2401 ctgccaatat aacttcgtag agcatagaac attgatttat gtaccagcct gatcaacata
     2461 taaatataaa tttttatgtt tcacgtaaaa caaaaaaaaa gacaaagctg ttatgatctt
     2521 agctttgtct tttatgtgtt agtcatcatg tatacgacat cctactacta acgtttatat
     2581 attcgaattt ttatttcata ataatcttca tgatctttgt ctttatgctc aacatgtaat
     2641 cctgtttgtt gaatcgcttg gatacttttg cctacctcat ctcgtgcttg agtgacatct
     2701 tgtgcaaagc gaaggttttg tgctttgact ttttcgggcc ccgttttttg gcgtactcta
     2761 tcttcagtct gtttcacatt tagcttttgt gcaatgactt gctcaatcaa cgcttcttgt
     2821 tcgctatcag acaatgataa taccgctctc gcatgacgtt cagtaatttt accttctctt
     2881 aagcgaagta gtactttcgg cgccaacttc aataaacgca acttatttgc aataaagctt
     2941 tgacttttac ctaaactttt tgccaattca ctttgcgttg tatcaccaat ttccaataat
     3001 ttcttatagg cttccgcttc ttcaacaaca gacaaatttt ctctttgaat attctcaatt
     3061 aatgcaacaa cagccgtctc ttcatcatcc atatcacgaa taataacgtc tgcttgaggt
     3121 aaatttagtg attgtattgc tctaaatcgg cgctctccag caataatttc aaacatatct
     3181 tcttcaatcg gtcttacaac aataggttgt agtaaaccat gttcatgtat tgattcagca
     3241 agttctttaa ttttatttgg ttcaaacacc tgtcttggtt gataacggtt gggaacgata
     3301 cgttcaattt gaatggattc aacattacta ttgcgatctt cttcaatatg tccaatgatg
     3361 tcatctttgt ttttcaaacc aaataatttt gaaaaaggtt ttttcattat ccattcgctc
     3421 cttttatgtt agttgtaaag gattatgcat tatttttcaa gtaaaggagt cttattaggc
     3481 gtccctggtt ttcttggata ctttttcggc gtctgtcttt ttttatcaat aatgaacatc
     3541 tggcgctctc cagcatcttc tggcaattca aaggtatgtg tttctgtaac attaccacct
     3601 aacacactaa ttgcaaattt tgcttcttct aattcttctt caccttttga agattttaat
     3661 gcaacaaact gtccaccttt tttaactagc ggtaaacaca attcacttaa cacggataat
     3721 ctagctactg ctcttgcagt aacaacatca taagactccc tgtagacacc cttaccaaat
     3781 gtttctgctc tatcgtgtat aaagctgaca tcctgtaatt gtaattctga cgctaaatgg
     3841 tttaaaaatt gaatacgctt atttaatgaa tcaacaatcg tcacttttaa ctgcggaaac
     3901 attattttta acggaatact tggaaaacca gctccagcgc ctacatcaca tatacttata
     3961 ggctgattaa aatcaaaata aaaactaggt gcaatggaat cataaaaatg tttcaaatat
     4021 acatcgtgtt catctgtaat acttgtcaaa ttcatctttt cattccattc aacaagtaaa
     4081 cgataatatg tttgaaactg ttgtttttga gtctcagtta attgaatatt atgttctttt
     4141 aattgttctg ctaaccattc tacagtcatt agtcactcac cctttggagt ttaccttgtt
     4201 ctaagtaaat caataatata gatatgtctg ctggatttac ccctgatatt ctagaagctt
     4261 gtgcaatatt taaaggtttt acttctgata atttttctcg cgcttcagtc gccaaactat
     4321 caatcttact ataatctaag tcttctggaa ttttcttctc ttccatacgc ttaactttct
     4381 caacttgttg tagtgattta ttgatataac cttcatattt tgtttgtatt tctacttgtt
     4441 cttcaacatc tgcattcaat tgatgttctt cttctaaaag ttctaaaatt atatcgtaag
     4501 tcatttcagg tctgcgtaat aaatcgatag ctaaaatacc atcttttaag cgagaaccac
     4561 catgttgttc aataatcgct tgcgtatgtt cgtttggttt aatacgaata tctgataaac
     4621 gcttaatttc cgcatcaatt tgctgacgtt tttcattaaa acgtgcatat ctttcttcag
     4681 aaatcatacc aagttcatat cccatatccg tcaatctcaa atcagcatta tcatgacgta
     4741 gtaacaaacg atattctgca cgtgatgtta gtaaacgata aggttcatta gtacctttag
     4801 ttacaagatc atcgattaag acaccaatat atgcatctga acgacttaat atcttttcgc
     4861 ctgtgtttaa cactttacct gcagcgttaa tacctgccat caatccttgt cctgctgctt
     4921 cttcataacc agatgtacca ttaatttgac ctgcagtata taagttttta atcattttcg
     4981 tttcaagtgt aggccataac tgcgttggca caatcgcatc atattcaatt gcgtagccgg
     5041 cacgcatcat atctgctttt tcaagacctg gtatcgtctc taacatttga cgttgcacat
     5101 gttcaggaag acttgtagac aatccttgca catatacttc atttgtatta cgaccttcag
     5161 gctctaagaa aagttgatgt cgcggcttat cattaaatcg aacaaattta tcttcaattg
     5221 aagggcaata acgtggcccg gttcctttaa tcatccctga atacattgca gatagatgta
     5281 aattatcatc gataactttg tgtgtttcag cattagtata cgttagccaa catggcaatt
     5341 gatctaatat atattctgtt gtttcaaagc tgaatgcacg acctacatcg tcacctggtt
     5401 gtatttcagt cttcgaatag tcaattgttt ttgaatttac acgcggtggt gtacctgttt
     5461 taaaacgaac aatatcaaaa ccaagttctc ttaaattgtc tgataatgtg attgatggta
     5521 attggtgatt tggtccactt gaatacttca tattacctaa aatgatttca ccacgtaaaa
     5581 atgttcccgt tgtaataatt actgctttag ataaatactc tgtaccaata tttgtacgta
     5641 cacctttaac ttcattatct tctataataa gttcgtctac cataccttgc attatatgca
     5701 aattttcttc atcttcaatc acgcgtttca tttcttgttg ataaagtact ttatctgctt
     5761 gcgctcttag tgctcttaca gcaggtcctt tacctgtatt taacattctc atttgaatgt
     5821 gtgttttatc gattgttttt gccatttgtc cacctaaagc atcaatttca cgaacaacga
     5881 tacctttagc tggtccacct acagatgggt tacatggcat aaatgcaata ttatctaaat
     5941 ttattgttag cattaatgtt ttagcaccac gtcttgcaga tgctaaacct gcttctacac
     6001 ctgcatgtcc cgcacctata acgattacat catattcttg aaccacaata taaacctcct
     6061 tatttgatat cttactagcc ttcttaagac ggtattccgt ctatttcaat tactatttac
     6121 ctaagcagaa ttgactgaat aactgatcga tgagttcatc acttgcagtc tcaccaataa
     6181 tttctcctaa tatttcccaa gttctagtta aatcaatttg taccatatcc ataggcacac
     6241 cagattctgc tgcatcaatc gcatcttgta tcgtttgtct tgcttgtttt aataatgaaa
     6301 tatgtcttga attagaaaca taagtcatat cttgattttg tacttctcca ccaaagaaca
     6361 aatctcgaat ttgtatttct aattcatcaa taccttcttg ttttaacatt gaagtttgaa
     6421 ttaatggcgt atcacctatc atatctttaa cttcattaat atctatgttt tgctctaaat
     6481 ccattttatt aacaattacg attacatctt catttttaac cacttcatat aatgtgtaat
     6541 cttcttgagt caatgcttcg ttattgttta atacaaataa aattaagtct gcttggctaa
     6601 gagcctttct agagcgttca acaccaatct tctctactat atcttctgtc tcacgtatac
     6661 cagcagtatc aactaatctt aatggcacgc cacgaacatt gacgtactct tctaagacat
     6721 ctctagtagt acctgctacc tcagttacaa tcgctttatt atcttgtatt aaattattta
     6781 acatcgatga tttacctacg tttggtttac caacaataac tgtagataaa ccttcacgca
     6841 taattttacc ctgcgcaccg gtatctaata aacgattaat ttcctgtttg atttctttag
     6901 actgctctaa aagaaattca gtagtcgcat cttcaacatc atcgtattca ggataatcaa
     6961 tattcacttc cacttgagcg agtatctcta atatagattg acgttgtttt ttgattaagt
     7021 cacttagacg accttcaatt tgattcatcg caactttaga agctctatct gtcttcgagc
     7081 gaataaagtc cataactgct tcagcttgag ataaatcaat acgaccattt aaaaaggcac
     7141 gttttgtaaa ttcacctggc tcagccattc tagcgccata tgtcatagta agttccagca
     7201 ctctattaat cgttaaaata ccaccatgac aattaatttc tataatatct tcgcgtgtaa
     7261 atgtttttgg cgctcttaac acagacacca taacttcttc aatcacttct ttagactctg
     7321 gatcaataat atgaccgtaa ttaatcgtat gtgatggaac atcatttaaa agatgttttc
     7381 ctttatataa tttgtcagca atttcaacgg cttgcggtcc agacaatcga acaattccaa
     7441 ttgccccttc acccattggt gttgaaatac tcgtaattgt atctaaatcc atattgctac
     7501 tcgcctcctt caacgatgtg aatacatttt aaagtaagtt attataaccc taaggtcagt
     7561 cttacgtttg tctgaggtaa gacttcggga tgtgttgagt ggttaatgtt ttccttcccc
     7621 taccctatcc ttacttaatc tttttattaa aaactttggc aattttaagt acgtgctcaa
     7681 gactattctg tatttgtaaa gtcgtcatat ctttagctgg ctgtcttgct attacaataa
     7741 tatctttggc caatatatgc gacttatgta ctttgaaatt ttcacgtatt gctcttttaa
     7801 tcttgtttct taacactgca ttacctagtt ttttagaaac actaatacct aagcgaaaat
     7861 ggtctatttc tttattatta caagtgtata caacaaattg tctgttggct acagaatgac
     7921 cttttttata tattctctga aaatctgcat tctttttaat tcggtaagct ttttccaata
     7981 acatcactcg cttatttatc gtttttattt gaagctatat ttaaacttct attgagctta
     8041 taacataaat ttctatttat tcttaattta aacgaaaaaa aagatcactg atgagtcagt
     8101 gatcttatgc agataaaact ttacggcctt tacgacgacg gcgcgctaaa actttacggc
     8161 cattttttgt gctcatgcgt tttctgaaac catgaacttt actatgttta cgtttatttg
     8221 gttgataagt acgttttacc atgcaaaaca cctccatctt tatacagtta tctaatatac
     8281 cagattatgc agactctgat cactttaata gataagattt tgcgtttctt agattaaaat
     8341 tctcgtatta gctcattgat tatctagtca taattcaagc aactactaca atataacaaa
     8401 atcctattta taacgcaagt tcattttata ctactgctca atttttttac ttttatcgat
     8461 taaagataga aatacacgat gcgagcaatc aaatttcata acatcaccat gagtttggtc
     8521 cgaagcatga gtgtttacaa tgttcgaaca ccttatacag ttcttataca tactttataa
     8581 attatttccc aaactgtttt gatacactca ctaacagata ctctatagaa ggaaaagtta
     8641 tccacttatg cacatttata gttttcagaa ttgtggataa ttagaaatta cacacaaagt
     8701 tatactattt ttagcaacat attcacaggt atttgacata tagaggactg aaaaagtata
     8761 attgtgtgga taagtcgtcc aactcatgat tttataagga tttatttatt gatttttaca
     8821 taaaaatact gtgcataact aataagcaag ataaagttat ccaccgattg ttattaactt
     8881 gtggataatt attaacatgg tgtgtttaga agttatccac ggctgttatt tttgtgtata
     8941 acttaaaaat ttaagaaaga tggagtaaat ttatgtcgga aaaagaaatt tgggaaaaag
     9001 tgcttgaaat tgctcaagaa aaattatcag ctgtaagtta ctcaactttc ctaaaagata
     9061 ctgagcttta cacgattaaa gatggtgaag ctatcgtatt atcgagtatt ccttttaatg
     9121 caaattggtt aaatcaacaa tatgctgaaa ttatccaagc aatcttattt gatgttgtag
     9181 gctatgaagt taaacctcac tttattacta ctgaagaatt agcaaattat agtaataatg
     9241 aaactgctac tccaaaagaa acaacaaaac cttctactga aacaactgag gataatcatg
     9301 tgcttggtag agagcaattc aatgcccata acacatttga cacttttgta atcggacccg
     9361 gtaaccgctt tccacatgca gcgagtttag ctgtggccga agcaccagcc aaagcgtaca
     9421 atccattatt tatctatgga ggtgttggtt taggaaaaac ccatttaatg catgccattg
     9481 gtcatcatgt tttagataat aatccagatg ccaaagtgat ttacacatca agtgaaaaat
     9541 tcacaaatga atttattaaa tcaattcgtg ataacgaagg tgaagctttc agagaaagat
     9601 atcgtaatat cgacgtctta ttaatcgatg atattcagtt catacaaaac aaggtacaaa
     9661 cacaagaaga atttttctat acttttaatg aattgcatca gaataacaag caaatagtta
     9721 tttcgagtga tcgaccacca aaggaaattg cacaattaga agaccgatta cgttcacgct
     9781 ttgaatgggg gctaattgtt gatattacgc caccagatta tgaaactcga atggcaattt
     9841 tgcagaagaa aattgaagaa gaaaaattag atattccacc agaagcttta aattatatag
     9901 caaatcaaat tcaatctaat attcgtgaat tagaaggtgc attaacacgt ttacttgcat
     9961 attcacaatt attaggaaaa ccaattacaa ctgaattaac tgctgaagct ttaaaagata
    10021 tcattcaagc accaaaatct aaaaagatta ccatccaaga tattcaaaaa attgtaggcc
    10081 agtactataa tgttagaatt gaagatttca gtgcaaaaaa acgtacaaag tcaattgcat
    10141 atccgcgtca aatagctatg tacttgtcta gagagcttac agatttctca ttacctaaaa
    10201 ttggtgaaga atttggtggg cgtgatcata cgaccgtcat tcatgctcat gaaaaaatat
    10261 ctaaagattt aaaagaagat cctattttta aacaagaagt agagaatctt gaaaaagaaa
    10321 taagaaatgt ataagtagga aactttggga aatgtaatct gttatataac agcactaatg
    10381 ataacaatca ttttttacat ttctatatgc taatgtggca agatgagcaa aactcatttt
    10441 gtggataatg tttaaaagtc atacacacca tacacaagtt atcaacatgt gtataacttc
    10501 gccaaatcta tgtttttaag acttatccac caatccacag cacctactac tattactaag
    10561 aacttaaaac ctatataatt atatataaac gactggaagg agttttaatt aatgatggaa
    10621 ttcactatta aaagagatta ttttattaca caattaaatg acacattaaa agctatttca
    10681 ccaagaacaa cattacctat attaactggt atcaaaatcg atgcgaaaga acatgaagtt
    10741 atattaactg gttcagactc tgaaatttca atagaaatca ctattcctaa aactgtagat
    10801 ggcgaagata ttgtcaatat ttcagaaaca ggctcagtag tacttcctgg acgattcttt
    10861 gttgatatta taaaaaaatt acctggtaaa gatgttaaat tatctacaaa tgaacaattc
    10921 cagacattaa ttacatcagg tcattctgaa tttaatttaa gtggcttaga tccagatcaa
    10981 tatcctttat tacctcaagt ttctagagat gacgcaattc aattgtcggt aaaagtgctt
    11041 aaaaacgtga ttgcacaaac aaattttgca gtgtccacct cagaaacacg cccagtacta
    11101 actggtgtga actggcttat acaagaaaat gaattaatat gcacagcgac tgactcacac
    11161 cgcttggctg taagaaagtt gcagttagaa gatgtttctg aaaacaaaaa tgtcatcatt
    11221 ccaggtaagg ctttagctga attaaataaa attatgtctg acaatgaaga agacattgat
    11281 atcttctttg cttcaaacca agttttattt aaagttggaa atgtgaactt tatttctcga
    11341 ttattagaag gacattatcc tgatacaaca cgtttattcc ctgaaaacta tgaaattaaa
    11401 ttaagtatag acaatgggga gttttatcat gcgattgatc gtgcctcttt attagcgcgt
    11461 gaaggtggta ataacgttat taaattaagt acaggtgatg acgttgttga attgtcttct
    11521 acatcaccag aaattggtac tgtaaaagaa gaagttgatg caaacgatgt tgaaggtggt
    11581 agcctgaaaa tttcattcaa ctctaaatat atgatggatg ctttaaaagc aatcgataat
    11641 gatgaggttg aagttgaatt cttcggtaca atgaaaccat ttattctaaa accaaaaggt
    11701 gacgactcgg taacgcaatt aattttacca atcagaactt actaaaaata aatataaata
    11761 aaggatgacg tgattaatta aaacgtcatc ctttattttt tggcaaaaat aattctaggt
    11821 gcgtatgtaa aataaatttg gcagcatttt aaacagcaaa taaaagacgc caattaaatt
    11881 tatgacaaat gtatccaaaa tttaataagt gtgcttatat gccctttaaa tttaaaattt
    11941 taatagtcaa taacaagttg aatataaaag ttaaacgccg ttaaatagcg ttaaaaaatt
    12001 gaaaatgaca gtattgccaa aaaataagaa ttaatt
//
LOCUS       DAMQYT010000063        11884 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMQYT010000063 DAMQYT010000000
VERSION     DAMQYT010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11884
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.063"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            455..1327
                     /locus_tag="PGA33_001324"
     CDS             455..1327
                     /locus_tag="PGA33_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDG6473143.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
     gene            complement(1714..3093)
                     /locus_tag="PGA33_001325"
     CDS             complement(1714..3093)
                     /locus_tag="PGA33_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDG6473144.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            3373..3729
                     /locus_tag="PGA33_001326"
     CDS             3373..3729
                     /locus_tag="PGA33_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473145.1"
                     /translation="MLKQILLRATKISVLFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTVFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            3726..3911
                     /locus_tag="PGA33_001327"
     CDS             3726..3911
                     /locus_tag="PGA33_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473146.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(4007..4198)
                     /locus_tag="PGA33_001328"
     CDS             complement(4007..4198)
                     /locus_tag="PGA33_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473147.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(4209..4844)
                     /locus_tag="PGA33_001329"
     CDS             complement(4209..4844)
                     /locus_tag="PGA33_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDG6473148.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(4919..5836)
                     /locus_tag="PGA33_001330"
     CDS             complement(4919..5836)
                     /locus_tag="PGA33_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HDG6473149.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFVSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            6104..6634
                     /locus_tag="PGA33_001331"
     CDS             6104..6634
                     /locus_tag="PGA33_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473150.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(6700..8304)
                     /locus_tag="PGA33_001332"
     CDS             complement(6700..8304)
                     /locus_tag="PGA33_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDG6473151.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASSGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            8634..9398
                     /locus_tag="PGA33_001333"
     CDS             8634..9398
                     /locus_tag="PGA33_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDG6473152.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTKKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            complement(9456..9962)
                     /locus_tag="PGA33_001334"
     CDS             complement(9456..9962)
                     /locus_tag="PGA33_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDG6473153.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(10129..11442)
                     /locus_tag="PGA33_001335"
     CDS             complement(10129..11442)
                     /locus_tag="PGA33_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDG6473154.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     regulatory      complement(11519..11614)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11667..>11884)
                     /locus_tag="PGA33_001336"
     CDS             complement(11667..>11884)
                     /locus_tag="PGA33_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473155.1"
                     /translation="TRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKWWL
                     LLVISIIVLIIITVAMIVYEKYYTTPD"
BASE COUNT         4167 a         2217 c         1623 g         3877 t
ORIGIN      
        1 tgcccattaa cctatttttc ataaaatgtc atttaaacaa gttatttatt aaaattcact
       61 ttattacata aattatacaa ttaaaaagtt tcttcaaatt gtaaagatgc attaatcgag
      121 ttataatcat aatgattaag atggtagatt tagtcttata taactacatt tactaagatt
      181 taaatagatg agtacataat gtataaattg ggaatataca gtatgactta cggtaatata
      241 tcaatatatt gatatattat ttgcacatgg gagtatgcta ttttttatct atacatctta
      301 aaaaaattta cagttttaca attcaatatc attaaacaaa caacatattc agcttatttt
      361 tcattctctt acgttacaag ttgtaaatac catcgcgtta aacaacaata gccaacgcta
      421 ataaaatata ttgcgttaaa gtggagtgat taatatgtca aacgtactaa cagaaataga
      481 tagtcaatat ccatatatga ctaaaaatga aaaaaagata gctaaattta tactaaattc
      541 acctcaaaag gtgattaaaa tgaggtctca agacttagca agcttattag atattagtac
      601 atcatcagtc attcgattca gcaagaaaat tactgatggc ggttttcacg acttgaaaat
      661 aaatatatct aaatatgttc ctaaagcatc atcaatttac aatgttgaat taatgaataa
      721 cgaaagcaca gaatctttaa gaactaaact ccatacacgt actacacgcg cacttaatca
      781 tgcaaataac gaattaaatg ataaaactat tgatcaaata tgtcattgtt taaaacgttc
      841 tgagacgatt tttatatatg gttttggcgc atcttttgta gttgctaccg acttatacca
      901 aaagttatca agaataggtc ttaatattca acttgttcaa gaaacacata tttttgctac
      961 attattagca actcacaatt cgaacgacag tgttattctt attactaaca atggtacgca
     1021 aagtgaaatg caatcaatgg ttaaagttat tgatgactac catataccta taattacaat
     1081 tactagtaca agggataatc ccgtagcaca ggcatcaaat attgttttaa cttatgggaa
     1141 aactgatgaa aatgagatgc atatgggagc aacaacttca ttatttgctc aaatgttcac
     1201 tattgatatt ttatattatc gttatgttgc attaaattat cacgcatcat tagatttcat
     1261 aacacaatct aaaatggctt tagataacta tcgtaaacat ttatcaaata ttaactttaa
     1321 acattaacag tcttatataa attctttaat catgtcatat aataatttat gcatcatttt
     1381 caaagcttac atgtctttta tcttgtataa catacgcata tgaccaatga tttgataagg
     1441 ccattcaatt tcttatatta aaaacaaacg cagttgatta acggattcat tccatatatc
     1501 aattgcgttt gttattttaa taatgctatt ttttatttta aagcccacag taaaaactaa
     1561 cgataatatt gccacttata agtttttcac actacaactt cgatttaagc tatatttata
     1621 aaaaaatgcg cctactcata acattctttg gctatttaac tttaataagc tagggccaag
     1681 tttgtcataa gataagcgca cattataatc atactatttt gcttttacat tatttttatt
     1741 atatctaata aaataataaa tcgttacaat cgttaagaag atgacaccaa taagcactga
     1801 tattctagtt tcaacattaa ataccatacc aaccaatact aatattaaga ataagattgt
     1861 taaatagtta gttactgcgc caccaggcat tttgaatggg tgaccttcca attcttcagg
     1921 gtgtaatttt ctaaaacgta aatgactgaa taaaatcata aaccaaggaa tcatacctgg
     1981 taaaatagac gcactataaa cgtatacgaa aatactcttc gctccatcga tatataaagg
     2041 taaaattacg tttaataaag caccaattaa aataccaaga gatactgcta caacagtgta
     2101 caatggtaca ccatttctca tgattttagt aaatattttc ggcatttcac ctttatgagc
     2161 aagtgtataa atcatacgac tcgcactgaa aatacctgag ttacatcctg acattgctgc
     2221 agttaataca acaaagttaa ttaatccagc tgcaaatgta ataccgattt tagcaaatgt
     2281 tgcaacaaac ggacttccaa tgtctcctaa ttgattccaa ggatacactg aaacaataac
     2341 aaaaatagcg cctaaataga aaattaaaat tctccagata acaccattca ctgctttaac
     2401 gatatttttc tgaggatctt tagtttcacc tgccgtaata cctatcagtt ccacaccttg
     2461 gtatgaacca attacaattg ataatgcaaa gaagaaacca acaataccat ttggcataaa
     2521 tccgccattt gtccatagat tagaaatacc taccgcatgg ccaccatttc caaaaccaaa
     2581 gaaaataaga ccaaaaccag caataatcat taaaataatt gttacaactt taattaaagc
     2641 aaaccaaaat tcaaattctc caaacgcttt taccgagaat aaattcgctg ccattaataa
     2701 taaaatagca ataacaccag gaatccaagt tggcaattcc gggaaccaaa acttcatata
     2761 ttctcctact gcgatgacct cactcatacc aactacaatc cattggaata tattactcca
     2821 tgctgtcata taacctgctg caggatgtat ataatcactt gcaaatgttg caaatgatcc
     2881 tgttgtaggg tttaaataaa tcatttcccc cattgctctc atgattaaaa ataaaaagat
     2941 acccgcaatt aaatatgcaa ggataactga tgggcctgtc catttaattg tacttgtcgc
     3001 acccatgaaa agccccacgc caatcgtacc accaagtgca atcatttgaa tttgacgggc
     3061 actcaatccc ctttgtagtt cattgttctc cataacttct taccccatct ctgattgata
     3121 ctaaataaat ccataaaaaa acttattgcc gtaattatac tataaattat cgattttggt
     3181 acattaatat ttgcatacca tgcaatcgtt tacagaacta ctatagcagg cttttgcaac
     3241 tttgcaaaca attattttgt gatttttcta aaaaattaaa aaagttgctc aagtaccgtt
     3301 gagtcacata caacttcagc tattcttggt aattatgata taattttaat aatattaaaa
     3361 aggagtatat caatgttaaa acaaatactg ctacgtgcta caaaaatatc ggtactcttt
     3421 gctgtagcat ttttcatcat aaattatatc ggtatggaaa aacctgacat cctatattta
     3481 gttggaagaa ctattattgc aacacttgct ttcatactca tttgtcttac agtatttact
     3541 attattaatt ctccagagcg caagattaaa ctcggtacga ctttaccgat tgcattaatt
     3601 attggtatca ttttcggcgc catattttta acggtacaaa tcggtgtcat cactgggtta
     3661 atcatcggtg tgattgctac gtttatttgg gaattgattg aaaaaaataa aggaggtcgt
     3721 tcatcatgac aacagcatta attggtgtaa ttgtattaat cttactaatt attagctttg
     3781 tgccaaacta ccgtgctatg aaatcagcaa aagaacaagg aagtaatgca acaagagcta
     3841 caattatggt aggcatagat attatcttaa ttgtacttat catcgtgacg ttattattaa
     3901 aatatatgta actatttaaa ccaccctatc tcatacataa acatgaacgt tctaccattt
     3961 cacatacata tggtcgtcca tttaatgagg taaggtggtt ttctcgttaa agtttacctt
     4021 gtaatagtaa aaaatagcca attgttgcga gtattaagta aatcattgaa ccaacaacaa
     4081 aagatcttac ttgcacgcga aatggtgtct gtttgttaaa tataggacca atcaccaaat
     4141 ttccaacgcc taaaattact ggaccagcta gaaaaaacaa tataatgtaa cccatcatga
     4201 tacgcctcct atttcgtaaa gaaacgcgct acaatttgat cgtaatcaat atcttttgct
     4261 acataatcta cattagaaaa gtcctgtgcg cttgtcattt tattcgtatt aacaataaca
     4321 tctaatccag ctgcaatcgc ggctgttgca ccatttacag aatcttcaat agccaaacat
     4381 tgtgtcggca tataatttaa ttgttgtact gcagataaat ataattcagg gtcaggttta
     4441 acttgttcaa catcttctct accaacaact acctctatat aatcgtctag acctaatgct
     4501 ttaaaggtgg gcattatttc cgctttcaca ctacttgtgg caattgccat aggaatgtga
     4561 cgttgcttta aaaatgccat caatgattta atcgtatcaa tcatcggtaa tgttgtacta
     4621 gtaagatgat gttcttcata aagtttatct ttattttccg aaccaatcgc tttaattaaa
     4681 tggtcatgca aatctgtagc tgctcctcca atagaagaac gataaaaatc aatgcttata
     4741 ggatcggcat tatgcatctc taaatgttta ttaataacat taaataaatg ttgttccgta
     4801 tctattattg ttccatcgaa atcaaatata actgctctat acatatattt actcctctat
     4861 tttttctctt tatcaaagct taacatagtc cgatgaccac ttcattctat tagtgcaatt
     4921 ataacttttt tgaccaatac tttgctaaaa taggccctgt tatattatga atgaaactaa
     4981 acactgcgcc tggtactgct gcaattggat taaaatgcaa tgctgctaat gacacagcta
     5041 aaccagagtt ctgcattcca acttcaatac tgactgcttt ttgatcttgt cgatctaatt
     5101 ttaaaagacg agctaaccaa tatccaatcg tataccctaa tacgttatgt aaaataacta
     5161 ctaaaaatat taataatcct gtagttaaga tttggtgctt actgccacct acaactattg
     5221 ctaaaattaa tgaaatagca acaactgata taattggcaa agctgtagaa gctttttcag
     5281 caattttacg attaataatt tgcaaaacaa tacctaaagc aattggaatt aatacaactt
     5341 gaacaactga ccacaacata ctcacgaaag atacttccaa ccattcattt gcaaatagat
     5401 atattaacgc aggtgtaacg aatggcgcta gcaacgtaga gaccgtcgta atagaaacag
     5461 aaagtgctac gttagctttg gctaaataac tcattacatt acttgatgtc ccacccggac
     5521 aacatccgac taatattacg ccaacagcaa tatcaggtgg taaatgaaaa gactttgcaa
     5581 ttataaatgc taatgtgggc ataatactga attgtagaca gacaccaata attactgctc
     5641 taggtgcttt aaagaccatt ttgaaatcat taggtgtaat tgttaatccc atacctaaca
     5701 taacaatacc aagtaaataa ggtacccatt tacctaatgt cgcaacatgt tgtgggaaaa
     5761 taaatccaat aacagctgct actaacatcc ataccaaaaa actcttcgtg gcaaatatac
     5821 tcaatttttg aaacattgtt aacacatcct cgtcgataat attgaaaaat attaccataa
     5881 gaccagtaag gttacaatta cttttctgaa aattaagatt atttagtcat tctcttctta
     5941 ttgcaaatat tttatcactg ataaacaatt attttaaact tcattcaaaa gtcttagtga
     6001 ataaagaaaa aaagcgtggt aaaatcaact tgaacaaaat taagtcacct ttataaatat
     6061 taaacgtgta tttaatgcca tgtattcctc gggaggtcct cacatgttaa ttgataaatt
     6121 cgaaacatac atcatcaaca tcgccggttt aaatgaccgt actactagaa aaaaattaag
     6181 taagttatgc aaaagtgtac aattttgtga tgcgttgcaa ttctcaataa acaaacagtt
     6241 taatcagtat gtattagaaa ttagcttacc aaagcaacaa cttccatact tcatttcatt
     6301 tttaagtttt catcagtata gtattttcca agtactttca ccaaagaaaa taaatgaatt
     6361 acttgattct gataatttat atcaatctgc aaagcgtttc gatatcaata ttgatggctt
     6421 gcaagatgct ttcattaaag acaaagtcat cgatattatg aatatgttcc aaaatcacac
     6481 tgatatcact tatacgttga ataagtctca tgcacatatt atatgtacgc cagaaatatt
     6541 tgctaaattg ttacatacga ttgcaactag aaatatcgac attctatctg ccaattatag
     6601 atcgtcatct atgagcaaag ctcgtatatc ataataaaag acacatctat acgatgatca
     6661 tttacaatga ttgcgtatag tgatgtgtct ttttttgtat tatttatctt cggacgattg
     6721 atcctcaacc atttgttcaa atgcttcacg aacttgtggt actgtcatac ctactacaac
     6781 ttggatactc ttaccactct ttactaatcc gtgtgccatt tgttgatgcg tgaaatattc
     6841 cgtatctgca actttacttt catcataaac agttaagcgt aatcttgttg tacagttagt
     6901 aacatctttg atattttctt tgccgcctaa accgtcgagg taatatgcag ctttgtcttc
     6961 gtattcatta ccagatgaag cacgtttaga atctacgcta tcaccttttt tatttttgta
     7021 atcttgctta gaaaataatt taacttcctc ttctgttttc ttacgtccag gtaatggaat
     7081 atcaaatttc aaaattaaga atctgaataa gaagaagtaa atcgctacaa atatcagacc
     7141 gataataact tgtgcaacat aagtacccca atgttcttta cctaatggca accagtttgt
     7201 tgaaataaag tctaacaagc caccacccat gttacctaca acaccaaatg cgtacattaa
     7261 agtatccatt gatgctgcta aaactgcgtg taaaacaaat aagtatggtg cgatgaataa
     7321 gaatgtaaac tctaatggct cagtaatacc tacaacgact gctgttaatg tggcaggaat
     7381 aagtagtgcc gccacctttt tacgattttc tttaggtgtc gtagcataca ttgctaaagc
     7441 aatacctaaa catccaaata ctttcccatt tccttgaagc ataaagccat aagggaattg
     7501 ctcttttaat ggcttagtac tcttagcaaa ctcatttaaa tgttgtaacc attcagcttt
     7561 caagccgtga ttgactacta ctggtccaac ttcaattggt gcataaataa agtgatgtaa
     7621 tcctgttggt attaatacac gttctaagaa gtgatacaac catacaccga tatagcctga
     7681 agcaatgata aagtgttgca ttgaaccaat accgtgctga acagttggcc aaataacaca
     7741 agtaattgct gctaatggta acatcacaaa aaatgaaatc gtaactacaa atgttaaacc
     7801 ttggaataca ccaaccattt ctggtaatcg tttactatag tatctattgt gaatccaagt
     7861 aataatagct gaaataataa taccacctag aatattcgta tccaatgtgg caatacctgc
     7921 aattgatttt aaaccaggta cattttcaac gcctttttct aaattagcgc caaacgtatg
     7981 tggccattgt gttaaaatgg catttataaa tgtattaaac attaagtaac ccatcaatgc
     8041 tgcaagtgct gcatgacctg gtgctttttt agctaaagaa agtggtagac ctacaacaaa
     8101 tacaacttcc atatgcgtaa aaataaccca gccaccagat tctattactg accaaaattt
     8161 aaaccaaaac gtatgttgat ctgctaaact ccccatgatt gtaggatttt taaataatgt
     8221 cgcaaagcca agcactatcc caaagaaagc gaacattaat accggtacaa tcattgcact
     8281 accgaaacgc tttatcgcat tcatcttcta ttccctccat atcatctttc ctaacaatac
     8341 atctaattag attcatttat aaatagatgt cttactattt aaatataata tatagtaaac
     8401 gcttacacac ctacaacgac attgacgtat tttgaaagta ttttgtataa tcagattatc
     8461 ttttcatata gtgaaaattt tttcacgacc ttatatatga catcgttgta tttgtaatac
     8521 attcgtttta aacgcataat caaacctata tcaatacaca aatatatata atgacataca
     8581 agattttaat gtaataacga tctattacac atttattttc aaggaggttg aatatgtttt
     8641 tagatgaaca cattaatcga aactttgata aacttaatga taatgattta catatcgctc
     8701 actttatcaa tacacatata gatgaatgta aaaatatgaa aatacaagat ttagcgcaat
     8761 tcacacatgc ctcgaatgca accattcaca gatttacacg caaattaggt tttgacggtt
     8821 atagtgattt taaatcgtac cttaaatttg aaagtaataa gacacaccaa ttaccttcta
     8881 actctatcga tagtttcaaa caagaaatcg atagtacatt cagttattta gaacgcgttg
     8941 attaccagct tcttactaaa aaaatgcata cagcttcaac aatatatttg tatggtacag
     9001 ggcgtgcaca gttaaacgtt gcttcagagg cacagcgtat attgttaacg ttacataatc
     9061 atgtcattgt acttcatgat gaacatgaac ttaaaatgat tttgaacaag agtgaaccta
     9121 atgatttatt ctttattatt tcattatcag gtgaaacaca tgaattatta gaagtaacaa
     9181 acttgcttca acttagacaa aaatacttta tttctgttac tacaatgaaa gataataccc
     9241 ttgctcaaaa agcaaattac aacgtctatg tatccagtaa cacattctac ttaactgatg
     9301 gtacagacta ttctagcttc atcagttatc atatattctt tgaaacactt gtaagaaaat
     9361 ataatgagta tttactacat aatgatattt caaattagaa aaaaggaagt tatgatttaa
     9421 tggccttgag cctatcataa cttcctttta tcattttatt gttgtgttga tgtttcgata
     9481 acgtggtaca tcttatcaaa catcaattcg aaaccatgca ccatggcatc atgatattct
     9541 tttttctttt gcttgtattc taaattagta aatcgtcttt ctttttcaac taatgaacga
     9601 taataaaata gcatttgggt gccacctgtt tcacgttcaa aaaattctac ctcaatgaca
     9661 tcttgcgttt cacttagtcc aggcataccg atagtcatct taacgtattc atccataact
     9721 aaagattcat aaatgccttc aatcacattt actttgccat tacgttgttg atctacaata
     9781 cgatatttac cgccttcttt aacgtccgct tcaatctctt tattcgttct ggctgatgtc
     9841 ataaaccatt gtttcaacaa atctttcttt gtccaagctt cgtatactaa ctctggagaa
     9901 aatttataaa gcttttcaat ttcaacttcg acatgttcat tctctacatt aaattttgcc
     9961 actgttgtcc acccactttc gctcttactt ttattttaac gtatttttgc tcagttccaa
    10021 acatagatga tcatcatttt taaaagatta gcgttatacg gtgagtacaa catgatctgt
    10081 taatataaca agccacctta cttggctaca tcgatatatt gttaagcatt aatgtttcat
    10141 ttcttgacta gtgttctttt ttagctttgg aaaattaaat aaaatcgcaa taagtccgca
    10201 tacacctaat aatataggat aaatgctgta tgggaataac attaacggtg aaataccagc
    10261 tacaccagcc gctgaaatga cttgcgggct atatggtaat aaaccttgga agcagcctcc
    10321 aaatatatca agaatacttg ctgatttcct tgaatctaca tcatattcat ctgcaatatt
    10381 tttagctaaa ggacctgaca taataataga gatggtgttg tttgccgtgg caatatctgc
    10441 gacacttacc aaactagcaa ttcctaattc tgcgccacgc tttgatttca ctttagagcg
    10501 aacaaattgc aacaaccatt caataccacc attgtgttga ataataccga ctaaaccacc
    10561 aattagcaac gcaatcatag caatatcttc catgcttata atacctttgg acactgcatc
    10621 tagtagcccc atccaaccga atgaaccatc tatgagacca atgattccgg ctaataatgt
    10681 tccgccaatc aatacgataa tgacatttac acctaataat gctaatacca atactaagat
    10741 atacggtaca actttaatta gattataatc atagttttta gcatgattta aagaaatgcc
    10801 attcgttaag aaatacagaa taataatcgt taaaatagca cctggcaata caattttaaa
    10861 gtttactctg aatttatctt tcattttcgt atgttgtgtt ctaaccgcag caattgttgt
    10921 atctgaaatc attgatagat tatcgccgaa cattgcacct ccaacaactg tagccattgc
    10981 tagcgcagtt ggtacatctg tcgcttgtgc aaatccgtaa ccaacaggtg ctattgcagc
    11041 aacagttcct acagacgtcc ccatagatat agaaacaaac atacaaatca caaacaatcc
    11101 tacaataatt aaattttctg ggattaatga tagtcctaaa ttaactgtcg actttacgcc
    11161 acccattttt tcagctgtat ttgaaaatgc acctgctaaa ataaaaatca acatcattaa
    11221 aacaatgttt gaatggcctg cacctttcgt gaagacctca acttttttag caaatgattc
    11281 ttttcgattc attaataacg ccacaattac cgttatcgta attgcaacat ttaatggcat
    11341 tgaagtaaaa tcacctgtga taatacctac gcctaaaaac aacgccacaa ataataacaa
    11401 ggggaataat gcccaagcat tgctcttttt atgtacttcc atccttttta cctgctttcc
    11461 aattaaaaat acctctttct cacaaacgat gaagaaagag gttttcatgt gctttacctg
    11521 cttatcttca aaccattacg gttactggaa ttggcacatt cgagatgttg ccgaggcttc
    11581 atagggccag tccctccacc tctctagata agtgatgctt atttacgttt acgttacaag
    11641 ataatcctta gtacgtcaat cataaattaa tcaggagtcg tataatattt ttcataaaca
    11701 atcattgcta ctgtaataat aatcaaaaca ataatgctaa taacaagtaa aagccaccat
    11761 ttaagcatta atgcaataaa aatgaacacg atagacacac ttactaatat taatgatatg
    11821 actttaaatt gctgaacacg ttgcttggag atgactttca actgtttgtt tgatagacgc
    11881 gtat
//
LOCUS       DAMQYT010000064        11836 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMQYT010000064 DAMQYT010000000
VERSION     DAMQYT010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11836
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.064"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            101..1159
                     /gene="hisC"
                     /locus_tag="PGA33_001337"
     CDS             101..1159
                     /gene="hisC"
                     /locus_tag="PGA33_001337"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDG6473156.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            1510..2052
                     /locus_tag="PGA33_001338"
     CDS             1510..2052
                     /locus_tag="PGA33_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HDG6473157.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLRESGFFRHLKVMPYAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(2676..3593)
                     /locus_tag="PGA33_001339"
     CDS             complement(2676..3593)
                     /locus_tag="PGA33_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDG6473158.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            3684..3785
                     /locus_tag="PGA33_001340"
     CDS             3684..3785
                     /locus_tag="PGA33_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473159.1"
                     /translation="MVINEIDFYRVGRENIVIAVYNEHIKFLFILIS"
     gene            complement(3863..5368)
                     /locus_tag="PGA33_001341"
     CDS             complement(3863..5368)
                     /locus_tag="PGA33_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDG6473160.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAISPLIIKAMKGIR"
     gene            complement(5709..6209)
                     /gene="queF"
                     /locus_tag="PGA33_001342"
     CDS             complement(5709..6209)
                     /gene="queF"
                     /locus_tag="PGA33_001342"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDG6473161.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            complement(6229..7095)
                     /locus_tag="PGA33_001343"
     CDS             complement(6229..7095)
                     /locus_tag="PGA33_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDG6473162.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
     gene            7896..8294
                     /gene="nrdI"
                     /locus_tag="PGA33_001344"
     CDS             7896..8294
                     /gene="nrdI"
                     /locus_tag="PGA33_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDG6473163.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            8257..10362
                     /gene="nrdE"
                     /locus_tag="PGA33_001345"
     CDS             8257..10362
                     /gene="nrdE"
                     /locus_tag="PGA33_001345"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDG6473164.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            10480..11451
                     /gene="nrdF"
                     /locus_tag="PGA33_001346"
     CDS             10480..11451
                     /gene="nrdF"
                     /locus_tag="PGA33_001346"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDG6473165.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
BASE COUNT         4200 a         1789 c         2050 g         3797 t
ORIGIN      
        1 tgatgtgaaa atagtagcat tgcattttag aaacatagag cgatataatg aatataagtt
       61 ttttgaaatt tcagttaatt ctaaggaggt tgtttttatt atgaaagaac aacttaatca
      121 actatcagca tatcagcctg gtttatctcc aagggcattg aaagaaaagt atggcattga
      181 aggagattta tataaacttg catcaaatga aaatttgtat ggaccatcgc ctaaagttaa
      241 agaagcgata tcagcacact tagatgagtt atattattat cctgaaacag gatcaccgac
      301 attaaaagcg gcgattagta aacatttaaa tgtagatcaa tcacgcattt tatttggtgc
      361 gggattagat gaagttatat taatgatttc tagagctgta ttaacgccag gggatactat
      421 tgttacaagt gaagcgacat tcggtcaata ttatcacaat gcgattgttg aatcagctaa
      481 tgtgatacaa gtacctttaa aagatggtgg cttcgattta gaaggtattt taaaagaagt
      541 taatgaagat acgtcattgg tatggttatg taatccaaat aatcctacag gtacatattt
      601 taatcatgag agcttagatt cgtttttatc tcaagtacct ccacatgtac cagtaattat
      661 agatgaagct tattttgaat ttgtgacagc agaggactac ccggatacac ttgctttgca
      721 acaaaaatat gacaatgctt tcttattacg tacattttca aaggcgtatg gattagcggg
      781 tttacgtgta ggatatgtgg tagcaagtga acatgcgatt gaaaaatgga acatcattag
      841 accaccattt aatgtgacac gtatatctga atacgcagca gttgcagcac ttgaagatca
      901 acaatattta aaagaggtaa cacataaaaa tagtgttgaa cgcgaaagat tttatcaatt
      961 acctcaaagt gagtatttct tgccaagtca aacgaatttt atatttgtaa aaacaaagcg
     1021 ggtaaatgaa ctttatgaag cacttttaaa tgtagggtgt attacgcgac catttccaac
     1081 tggtgttaga attacaattg gttttaaaga acaaaatgat aaaatgttag aagttttatc
     1141 aaactttaaa tacgaatagt aagtggggag tgggacagaa atgatacttt cgcaaaattt
     1201 atttcgtcgt cccaccccaa cttgcattgt ctgtagaaat tgggaatcca atttctcttt
     1261 gttggggccc cgccggcaag gttgactaga attgaaaaaa gcttgttaca agcgcatttt
     1321 cgttcagtca actactgcca atataacttt gtagagcatt gaacattgat ttatgtctca
     1381 agctcaatgc agtgtgaatg atgaggtgag agtattcagt gtaaaaagca acaatagatg
     1441 atattgtttt gtatcaattg cttttttgct atactgaatc aatactgata ttttgaggag
     1501 aagattaaaa tgacccgtaa atcaatcgcg attgatatgg atgaagtatt ggcagataca
     1561 ttaggagaaa tcattgatgc tgtcaatttt agagcggatt taggtattaa aatggaagct
     1621 ttgaatggtc aaaaacttaa acatgttatt cctgaacatg atggattaat tacagaagta
     1681 ttgagagaat caggcttctt cagacatctt aaagtgatgc cgtatgcaca agaagttgtg
     1741 aaaaaattaa ctgaacatta tgatgtatat attgctacag cagcaatgga tgtaccaaca
     1801 tcatttagtg ataaatatga atggttacta gagttctttc catttttaga tcctcagcat
     1861 tttgtttttt gtggtagaaa aaacatcgtt aaagctgatt atttaataga tgacaatcct
     1921 agacagcttg aaatttttac tggtacaccg attatgttta cagcagtgca taatattaat
     1981 gatgatcgat ttgaacgcgt aaatagctgg aaagatgtag aacagtattt tttagataat
     2041 attgagaaat aaaatatatc acttgaaaaa tttcatgtag aaaagatgat ggataggcta
     2101 taaagtaatt gtgactgaga tgaactttta tgtcttagac actacaacac tatattggca
     2161 gtagttgact gcggggcccc aacatagaga aattggaacg ccaatttcta cagacaatgc
     2221 aagttggggt gggccccaac ataaagaaat actttttctt tagaaattag tatttcttat
     2281 gcatgagtgt aactcatgca ttcatatttt taagtacaca ttagctgtga ctaatgataa
     2341 agaatcgcta cataatcaat cattagtcgt tctttatcat ttccgtcccg ctctcaataa
     2401 atgttagttt atcttattat tataaatcgg atgaatgtgt taatctatgg cagattacac
     2461 gtcatccgat tttttataga atttgaaaaa gacgcataaa ccactatgat ttaaaataca
     2521 acatcaatca ttttagtggc atgcgccaaa attatatgtc tgtttttgaa acagggtaat
     2581 agcttaaagc taataaaaac gaatataagg tgcgttgaat cttatgatta cactccaaac
     2641 ctaatataat atcgggttaa gatcattccg gatgcttaca aatcattgac agtaagtaac
     2701 tgaatggcat ttggtataac ctcaatatca ataggtgttt ctaatgaaat ttcgccatca
     2761 atatcaactt tcattgctgg atctgttgta agtgaaatct ttttaccagg tatatgctca
     2821 ataccttgag taatttcatt ccaattcatg ctatcacgct ttttaaaaat atcatttaaa
     2881 atactgaaac tttgttcatt aaaaatgaaa gtgttcagtt caccatcttg aggagacaaa
     2941 tcagtcaatg gtatacgact accaccaatg aatggaccat ttgctgttag tatcatggtc
     3001 gtttcgccag aatatgtctt atcatctatt gataattgat aattaaattg tgttggattt
     3061 agcagtgttt tgacagttga tccaatataa ctcaatttac caaatatatc ttttgaacca
     3121 tcttgtacgt tttcagcgtt ttgaacaatg agacctaagc caacaaagtt gagtgcatat
     3181 tgattattta ttttaattac atcgtatgta ccaacttgtg cagaaatcat ttgttcacta
     3241 gcttgtttat gattaggtgc tatatttagc gtttttgtaa aatcattaaa agtaccgcct
     3301 ggtaaaatgc caatagggag ttgaaggtca tgtgtcataa caccgtttat aagttcgtta
     3361 accgtgccat caccgccaag aataaataat atatctacat cttttgcata gtttttagtt
     3421 ttgatttctt ggcaatattt aataatgtca ccttcgtttt cactcaattg aatagaaaga
     3481 tgcttacaaa ttgaacttaa tgctgttgta acttccccaa taccttgatt aatatttttt
     3541 aatccactgt gttcatggta aaagaggaca ccatgtgtat atttattttc catagtttag
     3601 cctactttct aaaaattggt tcattaaata tatataccca cttttaattg ttaataccaa
     3661 aaatatgttt ttaaatagag aaaatggtaa taaatgaaat tgatttctat agagtgggac
     3721 gagaaaatat agttatagct gtctataatg agcatattaa gtttttattt atactgatat
     3781 cttgaattta attaatagaa acctataaaa aaacagtaag ccatttaaat gacttactgt
     3841 tttttgaatt aggccaacaa tattaacgta tacctttcat cgctttgatg attaaaggtg
     3901 agattgctaa tacaattgtt gtaacaataa ttgcaacaac acctaggaaa ataaagtaat
     3961 ttgtttgacc tagtggttct attaacttaa ctaaagtacc attgattgct tgtgcagaag
     4021 cgttagttaa gtaccaaata ctcatcattt gggcattaaa tgctttaggt gctaacttaa
     4081 cagcagcact attacccgtt ggtgataagc atagctcacc gataacacaa ataatgtacg
     4141 ataaaataac ccagttaact gaaaagtttg atgaacctga tgcataacct acaataccaa
     4201 ttagtatgta tgacgcacct gctaagaacg taccaattgc aaattttact ggcaggctag
     4261 gttgtttagt tccaagcttt tgccataaaa gtgaaataat tggagctagt aataaaataa
     4321 ataatgggtt aattgattgg aagatcgctt caccaaagtt tgttttccaa ccaaataagt
     4381 ttaatttcat atctgaatgt tcaattccat atatgtttaa tacattagac ccttgttctt
     4441 gaatagccca gaacaccatt ccaagaataa ataatggaat aaatgcttta acacgagaac
     4501 gttcagtatc agtgacatct ttacttctaa taattaaagt gaagtaaatg attggtaatg
     4561 caatacctaa tactaaaaca gtattactaa ctaagttaaa tgataatgag ttagttaatg
     4621 caccaataac gataattaat acaattgcta aaacaacact tccgataata agaccatact
     4681 ttttcttttc agctggtgtc aatgggttag taggtttcat accaacgcta cctaagtttt
     4741 tgcggttgaa aagtacatac catactaaac ctaatgccat accaactgct gcaatcaaga
     4801 atccgccgtg gaagttttta acattaacaa agtgttgcaa aataataggt gataataatg
     4861 cacccatatt aactgacata tagaaaataa caaaacctgc atccatacgt ctatcatttt
     4921 caggatataa acggccaacg atatttgaaa tgtttggctt cattaaacct gaaccaataa
     4981 tgatgaagaa cattgatgtg aataagccga ttaatgcaaa tggtaagctt aaacaaatat
     5041 gtccgataat aataaagact gcacctaata aagtagcgcc tctagtgcct gtaattctgt
     5101 cagcaatcca tccgcctggt attgatgtca tatagattaa tgaaccataa actgacataa
     5161 ttgacatagc tgttgtttta tcaattccaa ggccattatc tgttacggca aagtacatgt
     5221 agaaaatgag tagggcacgc atgccataat aactaaacct ttcccagaac tctacaaaga
     5281 agagtacgcc tagtcctcga ggatgcccga aaaatcctgt ttgaggtatg tcttgaattt
     5341 gatttccatg ggagttttgt tgtgtcatgt atacatccca tcctttcttc ccctaataca
     5401 atagtttaag taaatttgat tgtgccttat caactaaata ttccaacaaa tcacaaaaaa
     5461 ataaaaatat tgagaatatt ctgttattaa gcgcgcacaa ttaattagag ataatataaa
     5521 aatatactta ttgacgttaa agtgcaatag gtttctataa aaatgttgtt taattcgtgt
     5581 actaatattc ttttggtgta ctttggtata aaatgttatt ttaatgtaac ctattatgta
     5641 tgctaaatga aaaacacacc aataagttag gaaaatcaac ttattggtgt gtttattaaa
     5701 tgatacattt aacgattatc tattttttcg ggatataaat catgattcat caaacgatgc
     5761 tcagccattt tttcatattt agaatttgga cgtccatagt ttgtataagg atcaatagaa
     5821 attccaccac gtggtgtgaa cttgccccag acttcaatat aatgtgggtc cataagctct
     5881 atcaaatcat tcataataat attcatacaa tcttcgtgaa aatcaccgtg atttctgaaa
     5941 ctaaataagt ataatttcaa agattttgat tcaaccattt taacatttgg aatatatgaa
     6001 atatagatag ttgcaaaatc tggttgccca gtaattggac ataatgatgt aaattctgga
     6061 cagttgaatt ttacgaaata gtcacgacct tgatgcttat tatcaaacga ttctaataca
     6121 tcaggacgat agtcaaaatt gtaagtattg tcttgatttc ctaataaagt tatatcttgt
     6181 aattcatctt gttgacggcc atgtgccata taaagcgctc ctttaaattt atttttttat
     6241 tattttggcg tctcggcgtg ctttttcaaa catgtaataa cttgcaccga taataacgac
     6301 gtaacctaat gttgcataga aatctggaga ttctccgaat agaataaatc caagtattgc
     6361 tgtgaaaatt atagatgcat acgtaaaaat agaaatatct tttgctgctg caaaactata
     6421 tgctaaagta acaccaattt gacccacagc ggcagctaag ccagccccta atagataaag
     6481 tatttgcatc tgactcattg gttcataagt atatgcagtg aaaggtatta aaacgatgac
     6541 agaaaataag gagaagtaaa atactatagt atatggtgct tctcttgtac taagtgctcg
     6601 aacacatgta tatgctgatg ctgcaaaaat acctgagaat aagccagcta atgatggaat
     6661 catagatgat gaaaattcag gtttcactat taaaagcata cctaaaatag caattatcat
     6721 tgctgtaatt tgatacttcc ttaccttttc atgtaagaaa acaatgctta ataaaatcgt
     6781 ccagaaagga ttgagtttca ttaatgaatc ggcatcacta agtaccatat gatcaatggc
     6841 ataaatattt aacaatacac caataagtcc aagtgttgat cgtgttatta ataagggttg
     6901 acttgaaagt ctgccaaaca ttggctgatg gtatttatat ataaaaaata atggaataaa
     6961 cattgctact aagtttcgtg ctaatgattt ttgaaaaaca ggaaggtcac ctgcaagtct
     7021 gaaaaacact gacataaaac tgaaaccaat agccgaaatt aaaatggcaa tgataccttt
     7081 tactttagga ttcaatttta tcgcctcttt tatataaaat taacgtattt atattagcat
     7141 aaaacaacat gttgtgcata aatagttgaa atttactata aaaagactat aatagactgt
     7201 agcgaacaaa cgttctgtgt ttatttgtcg gaataatagg gcattacact tttatgaatg
     7261 tttgtgttat tacataaaac aaatatcaat tcagtatcaa gctaataagc tttttcttga
     7321 tttctgttga tacaattgag attgacacag atttaaaaaa atcaagtgat atctactaaa
     7381 aaattttttt aaatttgttc aagtttttct aatttagtat tggtgcctag ttggaacgtt
     7441 ttacgaacat tcgattagaa aatggcactt taaatcatag tgtgtcttat gtataatgaa
     7501 acacataata tagtgttggt gaaacgaaaa agacacaata tcttgtgttt tgtatgcaaa
     7561 tgctttattt atgaagaaat tacatttaaa agtaatttaa cacagaaatt taatagttat
     7621 tatcaattaa tagtcatatt tttagaaaat gtactgagca aatggaagat atccaatgat
     7681 gtaaacacta catatagtga tttttataca ttcaacccat ataagctact attttctcaa
     7741 atataaatct atgcaattgg tttacatttg agaaaataag tagcttcatt atagttaata
     7801 caatgctgag ataaccatag taaccatgtt gttaaagcat tttttaattg gaatgactac
     7861 tttatttaaa agggttgaag aaagaaggtg atccaatgaa aataatatat ttttcattta
     7921 ctggaaatgt ccgtcgtttt attaagagaa cagaacttga aaatacgctt gagattacag
     7981 cagaaaattg tatggaacca gttcatgaac cgtttattat cgttactggc actattggat
     8041 ttggagaagt accagaaccc gttcaatctt ttttagaagt taatcatcaa tacatcagag
     8101 gtgtggcagc tagcggtaat cgaaattggg gactaaattt cgcaaaagcg ggtcgcacga
     8161 tatcagaaga gtataatgtc cctttattaa tgaagtttga gttacatgga aaaaacaaag
     8221 acgttattga atttaagaac aaggtgggta attttaatga aaaccatgga agagaaaaag
     8281 tacaatcata ttgaattaaa taatgaggtc actaaacgaa gagaagatgg attctttagt
     8341 ttagaaaaag accaagaagc tttagtagct tatttagaag aagtaaaaga caaaacaatc
     8401 ttcttcgaca ctgaaatcga gcgtttacgt tatttagtag acaacgattt ttatttcaat
     8461 gtgtttgata tttatagtga agcggatcta attgaaatca ctgattatgc aaaatcaatc
     8521 ccgtttaatt ttgcaagtta tatgtcagct agtaaatttt tcaaagatta cgctttgaaa
     8581 acaaatgata aaagtcaata cttagaagac tataatcaac acgttgccat tgttgcttta
     8641 tacctagcaa atggtaataa agcacaagct aaacaattta tttctgctat ggttgaacaa
     8701 agatatcaac cagcgacacc aacattttta aacgcaggcc gtgcgcgtcg tggtgagcta
     8761 gtgtcatgtt tcttattaga agtggatgac agcttaaatt caattaactt tattgattca
     8821 actgcaaaac aattaagtaa aattgggggc ggcgttgcaa ttaacttatc taaattgcgt
     8881 gcacgtggtg aagcaattaa aggaattaaa ggcgtagcga aaggcgtttt acctattgct
     8941 aagtcacttg aaggtggctt tagctatgca gatcaacttg gtcaacgccc tggtgctggt
     9001 gctgtgtact taaatatctt ccattatgat gtagaagaat ttttagatac taaaaaagta
     9061 aatgcggatg aagatttacg tttatctaca atatcaactg gtttaattgt tccatctaaa
     9121 ttcttcgatt tagctaaaga aggtaaggac ttttatatgt ttgcacctca tacagttaaa
     9181 gaagaatatg gtgtgacatt agacgatatc gatttagaaa aatattatga tgacatggtt
     9241 gcaaacccaa atgttgagaa aaagaaaaag aatgcgcgtg aaatgttgaa tttaattgcg
     9301 caaacacaat tacaatcagg ttatccatat ttaatgttta aagataatgc taacagagtg
     9361 catccgaatt caaacattgg acaaattaaa atgagtaact tatgtacgga aattttccaa
     9421 ctacaagaaa cttcaattat taatgactat ggtattgaag acgaaattaa acgtgatatt
     9481 tcttgtaact tgggctcatt aaatattgtt aatgtaatgg aaagcggaaa attcagagat
     9541 tcagttcact ctggtatgga cgcattaact gttgtgagtg atgtagcaaa tattcaaaat
     9601 gcaccaggag ttagaaaagc taacagtgaa ttacattcag ttggtcttgg tgtgatgaat
     9661 ttacacggtt acctagcaaa aaataaaatt ggttatgagt cagaagaagc aaaagatttt
     9721 gcaaatatct tctttatgat gatgaatttc tactcaatcg aacgttcaat ggaaatcgct
     9781 aaagagcgtg gtatcaaata tcaagacttt gaaaagtctg attatgctaa tggcaaatat
     9841 ttcgagttct atacaactca agaatttgaa cctcaattcg aaaaagtacg tgaattattc
     9901 gatggtatgg ctattcctac ttctgaggat tggaagaaac tacaacaaga tgttgaacaa
     9961 tatggtttat atcatgcata tagattagca attgctccaa cacaaagtat ttcttatgtt
    10021 caaaatgcaa caagttctgt aatgccaatc gttgaccaaa ttgaacgtcg tacttatggt
    10081 aatgcggaaa cattttaccc tatgccattc ttatcaccac aaacaatgtg gtactacaaa
    10141 tcagcattca atactgatca gatgaaatta atcgatttaa ttgcgacaat tcaaacgcat
    10201 attgaccaag gtatctcaac gatcctttat gttaattctg aaatttctac acgtgagtta
    10261 gcaagattat atgtatatgc gcactataaa ggattaaaat cactttacta tactagaaat
    10321 aaattattaa gtgtagaaga atgtacaagt tgttctatct aacaattaaa tgttgaaaat
    10381 gacaaacagc taatcatctg ctctgaatta gcagatgatt agactgctat gtctgtattt
    10441 gtcaattatt gagtaacatt acaggaggaa attatattca tgatagctgt taattggaac
    10501 acacaagaag atatgacgaa tatgttttgg agacaaaata tatctcaaat gtgggttgaa
    10561 acagaattta aagtatcaaa agacattgca agttggaaga ctttatctga agctgaacaa
    10621 gacacattta aaaaagcatt agctggttta acaggcttag atacacatca agcagatgat
    10681 ggcatgcctt tagttatgct acatacgact gacttaagga aaaaagcagt ttattcattt
    10741 atggcgatga tggagcaaat acacgcgaaa agctattcac atattttcac aacactatta
    10801 ccatctagtg aaacaaacta cctattagat gaatgggttt tagaggaacc ccatttaaaa
    10861 tataaatctg ataaaattgt tgctaattat cacaaacttt ggggtaaaga agcttcgata
    10921 tacgaccaat atatggccag agttacgagt gtatttttag aaacattctt attcttctca
    10981 ggtttctatt atccactata tcttgctggt caagggaaaa tgacgacatc aggtgaaatc
    11041 attcgtaaaa ttcttttaga tgaatctatt catggtgtat ttaccggttt agatgcacag
    11101 catttacgaa atgaactatc tgaaagtgag aaacaaaaag cagatcaaga aatgtataaa
    11161 ttgctaaatg acttgtattt aaatgaagag tcatacacaa aaatgttata cgatgatctt
    11221 ggaatcactg aagatgtgct aaactatgtt aaatataatg gaaacaaagc actttcaaac
    11281 ttaggctttg aaccttattt tgaggaacgt gaatttaacc caatcattga gaatgcctta
    11341 gatacaacaa ctaaaaacca tgacttcttc tcagtaaaag gtgatggtta tgtattagca
    11401 ttaaacgtag aagcattaca agatgatgac tttgtatttg acaacaaata acaattaaat
    11461 taaaagacct tcacatgtaa agggaaatag cgattcgttt cgtcttgtct cctacatgtt
    11521 gaaggtcttt ttttatgtgt atctaactca ttatgagtct gagtaagaaa tcaatgctct
    11581 aagatgtaca atgctattta tattggcagt agttggcggg gccccaacac agaagcaggc
    11641 ggaaagtcag ctaacaatat tgtgcaagtt ggcggggccc caacatagaa gcaggcggaa
    11701 agtcagctaa caatattgtg caagttggcg gggccccaac ataaaagcag gcggaaagtc
    11761 agctaacaat attgtgcaag ttcgggcggg gccccaacat aaagaaaaac tttttccttt
    11821 agaaattatc acttcc
//
LOCUS       DAMQYT010000065        11793 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMQYT010000065 DAMQYT010000000
VERSION     DAMQYT010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11793
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.065"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            340..1131
                     /locus_tag="PGA33_001347"
     CDS             340..1131
                     /locus_tag="PGA33_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDG6473166.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LERMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
     gene            1146..1790
                     /gene="trmB"
                     /locus_tag="PGA33_001348"
     CDS             1146..1790
                     /gene="trmB"
                     /locus_tag="PGA33_001348"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDG6473167.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
     gene            2260..3102
                     /locus_tag="PGA33_001349"
     CDS             2260..3102
                     /locus_tag="PGA33_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDG6473168.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVLGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDNGENIDAYILRETL
                     VDNN"
     gene            complement(3227..3538)
                     /locus_tag="PGA33_001350"
     CDS             complement(3227..3538)
                     /locus_tag="PGA33_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDG6473169.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            3625..4701
                     /locus_tag="PGA33_001351"
     CDS             3625..4701
                     /locus_tag="PGA33_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDG6473170.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            4766..5077
                     /locus_tag="PGA33_001352"
     CDS             4766..5077
                     /locus_tag="PGA33_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDG6473171.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            5177..6034
                     /locus_tag="PGA33_001353"
     CDS             5177..6034
                     /locus_tag="PGA33_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HDG6473172.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            6063..6659
                     /locus_tag="PGA33_001354"
     CDS             6063..6659
                     /locus_tag="PGA33_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDG6473173.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            6680..10504
                     /locus_tag="PGA33_001355"
     CDS             6680..10504
                     /locus_tag="PGA33_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDG6473174.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEKRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKPKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEVDEDTTSNIEDNTNRNASEMHVDAPKTQEY
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLSVNSLKTNDVNDNHVVEDSSMNEIEK
                     NNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAVSERMPASQATPSSRSDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSVDSTQTEIFEESQDDNQLENEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQSSFSNKNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            10528..>11793
                     /locus_tag="PGA33_001356"
     CDS             10528..>11793
                     /locus_tag="PGA33_001356"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDG6473175.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVILFMGAGDIQK"
BASE COUNT         4459 a         1596 c         2221 g         3517 t
ORIGIN      
        1 tactaagtac gtaaatgtta cagttcataa atttatgcta accatgaaaa tgggatagca
       61 attattttga aaactagagt ttatcgccta cagagattca aaaacatgta gttttagaca
      121 cggtaacatt atatgtcaag taagtgacta tagaatgtat ggtgtcaatg acagacatta
      181 cgatttaaat ttagtcgtgt aatgtaggca ttggttgagg atttacaata attatctgga
      241 cataatattt gtaccagtat ggtgttataa attagaatat atggctcttg aaaaacctat
      301 ttctagagcc attttctaat tgaaagtgag gtggacgagt tggagcagtt ttatcaatta
      361 gggtggacac ttgattcagc aggtggtgca tctggtgaag catatatggc tgaacaagat
      421 ggacaaaagt tgtttttaaa acgaaattca aatccattta ttgcggcatt atcagcagaa
      481 ggtattgtgc ccaaattagt atggacgaaa cgcatagaaa caggcgaggt tgttacagca
      541 caacattgga aaaatgggcg tgaactatct tcaaacgaaa tgaagcaaac aagagttgca
      601 catttattaa agaagataca caattctaga cctttattaa gtatgttaga gcgtatggaa
      661 atggaaccta ttactcctga gattatgctt aataaaatta atgcctcttt atcaagagaa
      721 gttttaacac atcatattgt gagaaaatca ttaacctatt tagaagagca tataccgagt
      781 ttagattcgc gttttttcac tgttgtacat ggtgatgtga atcataataa ttggttatta
      841 tccgatcgag atgaactatt tttagttgat tgggaaggtg caatgattgc agacccggca
      901 attgatatcg gaatgctgct ttacaactat gttccacaac aacaatggtc tgaatggctt
      961 gaaacatatg gtgttcaaga gagcttgaat ttaaataaac gtatgaaatg gtatacggta
     1021 attcaatcta taggtcttgt tcaatggtat gaagagcaaa aaagatataa ggatatgaac
     1081 acttggctta aattcctaaa tgaagttatg aatagtaaca tgtttattta agaggagaat
     1141 aaaatatgag agttcgatac aaaccatggg ctgaagatta tttaaaagat catcctgaat
     1201 tggttgacat ggacggtcaa catgcaggga aaatgactga atggtttgat aaaacgcaac
     1261 cgatacacat tgaaattggt tcgggtatgg ggcagtttat tacaacatta gctgctcaaa
     1321 atcctcatat taactatatt tcaatggaac gtgagaaaag tatcgtttat aaagtattgg
     1381 ataaagttaa agagatgggt ttaacaaatt taaagattat ttgtaatgac gccattgaat
     1441 taaatgaata ttttaaagac ggcgaagttt cgcgtattta tttgaacttt tcagatccat
     1501 ggccaaaaaa tcgtcatgca aaacgacgtt taacgtatca tacgttttta gcgttgtatc
     1561 aacaaatctt aaatgatgaa ggagatttac attttaaaac tgataacaga ggactatttg
     1621 cctacagttt agaaagtatg tctcaatttg gaatgtattt tacaaaaata aatctaaatt
     1681 tacatcaaga agacgatggc agtaatattt taacagaata tgaaaagaaa ttttcggata
     1741 aagggtcacg tatttatcgt atggaagcga aattccattc acaaaaatag agcacttatg
     1801 agggtatgta cacatacaat cataagtgtt ttttcgtact ataatggttc tatagataaa
     1861 ggttctataa taaatcggac tgatggaaaa agtttttaac ttttcatctg tccgattttt
     1921 tgattttgaa tataaaaaaa gcgcaattat ctctataatt aaaagtgact aaacaaataa
     1981 ctaaaggaga taatgcgcct atgaacaatg ttatactaaa tacctttgat ttaaaagaat
     2041 aaagcttgca ttttacttgt aaattttaat tattaattta aaaatacagg ccgatggtta
     2101 tgatgcattt acatataata tgattgcatt aatctttata agtagttaga agtgtattag
     2161 tgtggggcgt ttcgtttttg ttcgcattta tgaatagtga tatgatgtaa taaacacatg
     2221 ctaattgtaa taacgaatac agaaagaata ggtgatgtta tgaaaatcgg ggatatatct
     2281 attcattatc taaatggtgg caatacaaaa atggatggcg gtgcaatgtt tggtgttgtt
     2341 ccgaagccgt tgtggtcaaa gcaatacaat gcaaatgaac gaaatcaaat caatttaccg
     2401 acacatccaa ttttgattca aacggctcaa tataatttga ttatagatgc aggtattggt
     2461 aatggtaaat tatctgaaaa gcaattacgt aattttggag tagatgaaga aagtcatata
     2521 attgctgatt tggcaaatta taatttaacg ccaaaggata ttgattatgt gctaatgaca
     2581 catatgcatt ttgatcatgc tgccggtttg actgatcaag caggacatgc aatttttgaa
     2641 aatgcgattc atgttgtgca acaagatgag tggcatgagt ttattgcacc taatataaga
     2701 agtaaatcaa cttactggga taagaataaa ggcgattata gtaacaagtt gattttattc
     2761 gaaaaacatt ttgaaccggt tctgggtatc aagatgcaac atagtggagg tcatagcttt
     2821 ggccacacga ttattacgat tgaaagtcaa ggagataaag cagttcatat gggtgatata
     2881 ttcccaacta ctgcacataa aaatcctcta tgggtaacgg catatgatga ttatcctatg
     2941 caatcgattc gtgaaaaaga acgcatgata ccatatttta ttcagcaaca atattggttc
     3001 ttgttttatc atgatgaaaa ctactttgct gtaaaataca gcgataatgg tgaaaacata
     3061 gatgcatata ttttacgtga aacattagtt gataataact aaaataaaga tgtattacta
     3121 aacaaatttt caaaaataaa aaattgagcc acatccaatc ttactaatta gggtgtggct
     3181 catttttaag ttttacgatc caaatcaaat atggataaaa ttcgtattaa cgctctacaa
     3241 tgttaatgac ttcaccagta tatgcatctg cataaaaatc ataatgaata ttttgaccat
     3301 ttttaatagt tgtaattcca ccttgataaa ctaaacggta tttatcagtt tcaggatgaa
     3361 cgaatggttc ataaacgata tatgagcctt tgacattcat aaaatatgat tttacttcat
     3421 taagtacttt aacgggatta tagcgtttac gattaataaa ttggatgatt gaaatggtag
     3481 aaataattac aatggctact gcaattattg ttggaattat atatttcagt ttagtcatca
     3541 tttatgctcc cttgatttca aattcatatc attagtttac catattgaag atgatataat
     3601 aacttataag gagtgagaac ttttatgaac ataaataaaa aagtaacatt acaacgaatt
     3661 caaactttaa ctgagcttca tggggcacca ggttttgaag aagaagtaaa aaattatatg
     3721 actcagcaaa tggcgccgta cgtagatgaa tttattgaaa atcgtatggg tggatttttt
     3781 ggtgtgaaaa aatctaaaaa tccaaatgca aaacgtgtaa tgattgcagc acatatggat
     3841 gaaatcggat ttatgattac aaatatcact aaaaatggaa tgattcaatt cacaaattta
     3901 ggtggtgttg caaatgatat ttggcaagga caacgcttag taattaaaaa tagaaatggc
     3961 gataaaatta tcggtgttgt ttctaatata cctaaacatt ttcgtactgg tagtgaaggt
     4021 gcaccggaaa ttaaagattt aacattagat ataggtgctc aaaatgaaga tgaggtgcgt
     4081 gagcgcggaa tagatatagg agatacaatt gtacctcaca cgccattcac acagttatct
     4141 gaacatcgat atagtgctaa agcatgggat aatcgttatg gttgtgtctt ggcaattgaa
     4201 atactagaat tattaaaaga tatagaatta gatgtagact tgtatgttgg cgcaaatgtt
     4261 caagaagagg ttggattacg aggtgcgaaa gcatctgcag agatgataga cccagacgtt
     4321 gcatttgtag ttgattgttc acctgccaat gacgttaaag gaaaccaacc attatctggt
     4381 gaacttggta aagggacgtt aattcgcata aaagacggta caatgatttt aaagcctgta
     4441 tttagagact atttattaaa gttagtagaa gcacatgaca ttgaacatca atactatatg
     4501 tcaccaggtg gaacagatgg tggagaaatt cataaagcta atattggtat tccgactgca
     4561 gttattggtg tatgtgcacg atatattcat agtacagact cagtatttga tataagagac
     4621 tattttgcag ctagatcttt actttcagaa gccatttgta atttagataa taatcaaata
     4681 gaaacattac aatataaata atcgggtaat aacaactatt atctctaaat agttatatat
     4741 aatcattaat taaggagaca taaaaatgaa acaacttgaa tcagaacaac aatttgaatc
     4801 tttaaaacaa ggtgctacag tatttgaatt cactgcaggc tggtgtccag attgtagagt
     4861 gatagaacca gatttaccgg aattagaagc gagatatcct atgtttgact tcgtatcagt
     4921 agaccgtgat aaatttatgg atatttgtat tgaaaatggt attatgggta ttccaagttt
     4981 tctagtatat aaaaatggag aactgcttgg aagttatatt ggaaaagaac gaaaatcaat
     5041 tgaacagata gatgcatttt tagctcaata cgtgtaattt agactagaga aaaacggggt
     5101 aatactcgtt tttctctgtt actatgtgtt gatttattgt aaactattat aaggtgcgaa
     5161 attaggagtg ttacatatga atacctttca aatgagagat aaattaaagg aacgtttaag
     5221 ccatttagac gttgatttta aatttaatcg tgaagaagaa actttgcgta tttatcgaac
     5281 agataataac aaaggtatca cgattaaact taacgctata gtcgcaaaat atgaagataa
     5341 aaaagaaaaa attgtagatg aaattgttta ttacgttgat gaagctattg cacaaatggc
     5401 agataaaacc cttgagagta tatcatctag tcaaattatg cctgtcatta gagcgactag
     5461 ctttgataaa aaaactaaac aaggtgttcc ttttatctat gatgagcata ctgcagaaac
     5521 agcagtttat tatgcagtcg atttagggaa atcatatcgt ctaattgacg aaagcatgtt
     5581 agaggatttg aagttaactg aacaacaaat aagagaaatg tctctgttta atgttagaaa
     5641 attgtcaaat tcatatacga ctgatgaagt aaaaggtaat attttttatt ttattaactc
     5701 aaatgacggg tatgatgcaa gtaggatact aaatactgca tttttaaatg aaattgaggc
     5761 acaatgtcaa ggcgaaatgc tcgtagcagt gccacaccaa gatgtgttaa ttattgcaga
     5821 tatacgcaat aaaacaggat atgatgtgat ggcacattta acaatggaat ttttcactaa
     5881 aggtctagtt ccaattacat cattatcctt tggatataaa cagggtcatc ttgaaccgat
     5941 atttatttta ggtaaaaata ataaacaaaa aagagatcca aacgtgattc agcgtttaga
     6001 agcaaatcgt cgtaaattta ataaagataa atagaaataa ttggataagg agttttgtca
     6061 taatgaattt attttacaat cctaaatatg taggagatgt cgcattttta caaattgaac
     6121 cagttgaagg tgaattaaac tacaataaaa aaggtaatgt tgttgaaatt actaatgaag
     6181 gtaatgttgt aggttataat atttttgaaa tttcaaaaga tataacaatt gaagaaaaag
     6241 gtcatattaa attaactgat gaacttgtaa atgtattcca aaagcgtatt tcagaagctg
     6301 gttttgatta taaattaaat gctgatctat caccgaaatt tgtagttggc tacgttgaaa
     6361 ctaaagacaa acatcctgat gcagataaat taagtgtact aaatgtaaac gttggaaatg
     6421 acacattaca aattgtatgt ggcgcgccta acgttgaagc tggacagaaa gttgttgttg
     6481 ctaaagtagg tgcagtgatg cctagcggta tggtaattaa agatgctgaa ttacgtggtg
     6541 ttgcctcaag cggtatgatt tgttcaatga aagaattgaa tttacctaat gcacctgaag
     6601 aaaaaggtat tatggtatta aatgacagct atgaaattgg acaagcattt tttgaataat
     6661 taaggaaggt agtgaaaata tgagctggtt tgataaatta ttcggcgaag ataatgattc
     6721 aaatgatgac ttgattcata gaaagaaaaa aagacgtcaa gaatcacaaa atatagataa
     6781 cgatcatgac tcattactgc ctcaaaataa tgatatttat agtcgtccga ggggaaaatt
     6841 ccgttttcct atgagcgtag cttatgaaaa tgaaaatgtt gaacaatctg cagatactat
     6901 ttcagatgaa aaagaacaat accatcgaga ctatcgcaaa caaagccacg attctcgttc
     6961 acaaaaacga catcgccgta gaagaaatca aacaactgaa gaacaaaatt atagtgaaaa
     7021 acgtgggaat tctaaaatat cacagcaaag tataaaatat aaagatcatt cacattacca
     7081 tacgaataag ccaggtacat atgtttctgc aattaatggt attgagaagg aaacgcacaa
     7141 gccaaaaaca cataatatgt attctaataa tacaaatcat cgtgctaaag attcaactcc
     7201 agattatcac aaagaaagtt tcaagacttc agaggtaccg tcagctattt ttggcacaat
     7261 gaaacctaaa aagttagaaa atggtcgtat ccctgtaagt aaaccttcag aaaaagttga
     7321 gtcagataaa caaaaatatg ataaatatgt agctaagacg caaacgtctc aaaataaaca
     7381 attagaacaa gaaaaacaaa atgatagtgt tgtcaaacaa ggaactgcat ctaaatcatc
     7441 tgatgaaaat gtatcatcaa caacaaaatc aatgcctaat tattcaaaag ttgataatac
     7501 tatcaaaatt gaaaatattt atgcttcaca aattgttgaa gaaattagac gtgaacgaga
     7561 acgtaaagtg cttcaaaagc gtcgatttaa aaaagcgttg caacaaaagc gtgaagaaca
     7621 taaaaacgaa gagcaagatg caatacaacg tgcaattgat gaaatgtatg ctaaacaagc
     7681 ggaacgctat gttggtgata gttcattaaa tgatgatagt gacttaacag ataatagtac
     7741 agatgctagt cagcttcata caaatggcat agagaatgaa actgtatcaa atgatgaaaa
     7801 taaacaagcg tcaatacaaa atgaagacac taatgacact catgtagatg aaagtccata
     7861 caattatgag gaagttagtt tgaatcaagt atcgacaaca aaacaattgt cagatgatga
     7921 agttacggtt tcgaatgtaa cgtctcaaca tcaatcagca ctacaacata acgttgaagt
     7981 aaatgataaa gatgaactaa aaaatcaatc cagattaatt gctgattcag aagaagatgg
     8041 agcaacgaat aaagaagaat attcaggaag tcaaatcgat gatgcagaat tttatgaatt
     8101 aaatgataca gaagtagatg aggatactac ttcaaatatc gaagataata ccaatagaaa
     8161 cgcgtctgaa atgcatgtag acgctcctaa aacgcaagag tacgcagtaa ctgaatctca
     8221 agtaaataat atcgataaaa cggttgataa tgaaattgaa ttagcaccgc gtcataaaaa
     8281 agatgaccaa acaaacttaa gtgtcaactc attgaaaacg aatgatgtga atgataatca
     8341 tgttgtggaa gattcaagca tgaatgaaat agaaaagaat aacgcagaaa ttacagaaaa
     8401 tgtgcaaaac gaagcagctg aaagtgaaca aaatgtcgaa gagaaaacta ttgaaaacgt
     8461 aaatccaaag aaacagactg aaaaggtttc aactttaagt aaaagaccat ttaatgttgt
     8521 catgacgcca tctgataaaa agcgtatgat ggatcgtaaa aagcattcaa aagtcaatgt
     8581 gcctgaatta aagcctgtac aaagtaagca agctgtgagt gaaagaatgc ctgcgagtca
     8641 agccacacca tcatcaagat ctgattcaca agagtcaaat acaaatgcat ataaaacaaa
     8701 taatatgaca tcaaacaatg ttgagaacaa tcaacttatt ggtcatgcag aaacagaaaa
     8761 tgattatcaa aatgcacaac aatattcaga gcagaaacct tctgttgatt caactcaaac
     8821 ggaaatattt gaagaaagtc aagatgataa tcaattggaa aatgagcaag ttgatcaatc
     8881 aacttcgtct tcagtttcag aagtaagcga cataactgaa gaaagcgaag aaacaacaca
     8941 tccaaacaat actagtggac aacaagataa tgatgatcaa caaaaagatt tacagtcatc
     9001 attttcaaat aaaaatgaag atacagctaa tgaaaataga cctcggacga accaacaaga
     9061 tgttgcaaca aatcaagctg tacaaacatc taagccgatg attcgtaaag gcccaaatat
     9121 taaattgcca agtgtttcat tactagaaga accacaagtt attgagtcgg acgaggactg
     9181 gattacagat aaaaagaaag aactgaatga cgcattattt tactttaatg tacctgcaga
     9241 agtacaagat gtaactgaag gtccaagtgt tacaagattt gaattatcag ttgaaaaagg
     9301 tgttaaagtt tcaagaatta cggcattaca agatgacatt aaaatggcat tggcagcgaa
     9361 agatattcgt atagaagcgc ctattccagg aactagtcgt gttggtattg aagttccgaa
     9421 ccaaaatcca acgacagtca acttacgttc tattattgaa tctccaagtt ttaaaaatgc
     9481 tgaatctaaa ttaacagttg cgatggggta tagaattaat aatgaaccat tacttatgga
     9541 tattgctaaa acgccacacg cactaattgc aggtgcaact ggatcaggga aatcagtttg
     9601 tatcaatagt attttgatgt ctttactata taaaaatcat cctgaggaat taagattatt
     9661 acttatcgat ccaaaaatgg ttgaattagc tccttataat ggtttgccac atttagttgc
     9721 accggtaatt acagatgtca aagcagctac acagagttta aaatgggccg tagaagaaat
     9781 ggaacgacgt tataagttat ttgcacatta ccatgtacgt aatataacag catttaacaa
     9841 aaaagcacca tatgatgaaa gaatgccaaa aattgtcatt gtaattgatg agttggctga
     9901 tttaatgatg atggctccgc aagaagttga acagtctatt gctagaattg ctcaaaaagc
     9961 gagagcatgt ggtattcata tgttagtagc tacgcaaaga ccatctgtca atgtaattac
    10021 aggtttaatt aaagccaaca taccaacaag aattgcattt atggtatcat caagtgtaga
    10081 ttcgagaacg atattagaca gtggtggagc agaacgcttg ttaggatatg gcgatatgtt
    10141 atatcttggt agcggtatga ataaaccgat tagagttcaa ggtacatttg tttctgatga
    10201 cgaaattgat gatgttgttg attttatcaa acaacaaaga gaaccggact atctatttga
    10261 agaaaaagaa ttgttgaaaa aaacacaaac acaatcacaa gatgaattat ttgatgatgt
    10321 ttgtgcattt atggttaatg aaggacatat ttcaacatca ttaatccaaa gacatttcca
    10381 aattggctat aatagagcag caagaattat cgatcaatta gagcaactcg gttatgtttc
    10441 gagtgctaat ggttcaaaac caagggatgt ttatgttacg gaagcagatt taaataaaga
    10501 ataattatga gtaaggagtt ttatataatg acacactatc attttgtcgg aattaaaggt
    10561 tctggcatga gttcattagc acaaatcatg catgatttag gacatgaagt tcaaggatcg
    10621 gatattgaga actacgtatt tacagaagtt gctcttagaa ataaggggat aaaaatatta
    10681 ccatttgatg ctaataacat aaaagaagat atggtagtta tacaaggtaa tgcattcgcg
    10741 agtagccatg aagaaatagt acgtgcacat caattgaaat tagatgttgt aagttataat
    10801 gattttttag gacagattat tgatcaatat acttcagtag ctgtaactgg tgcacatggt
    10861 aaaacttcta caacaggttt attatcacat gttatgaatg gtgataaaaa gacttcattt
    10921 ttaattggtg atggcacagg tatgggattg cctgaaagtg attatttcgc ttttgaggca
    10981 tgtgaatata gacgtcactt tttaagttat aaacctgatt acgcaattat gacaaatatt
    11041 gatttcgatc atcctgatta ttttaaagat attaatgatg tttttgatgc attccaagaa
    11101 atggcacata atgttaaaaa aggtattatt gcttggggtg atgatgaaca tctacgtaaa
    11161 attgaagcag atgttccaat ttattattat ggatttaaag attcggatga catttatgct
    11221 caaaatattc aaattacgga taaaggtact gcttttgatg tgtatgtgga tggtgagttt
    11281 tatgatcact tcctgtctcc acaatatggt gaccatacag ttttaaatgc attagctgta
    11341 attgcgatta gttatttaga gaagctagat gttacaaata ttaaagaagc attagaaacg
    11401 tttggtggtg ttaaacgtcg tttcaatgaa actacaattg caaatcaagt tattgtagat
    11461 gattatgcac accatccaag agaaattagt gctacaattg aaacagcacg aaagaaatat
    11521 ccacataaag aagttgttgc agtatttcaa ccacacactt tctctagaac acaggcattt
    11581 ttaaatgaat ttgcagaaag tttaagtaaa gcagatcgtg tattcttatg tgaaattttt
    11641 ggatcaatta gagaaaatac tggcgcatta acgatacaag atttaattga taaaattgaa
    11701 ggtgcatcgt taattaatga agattctatt aatgtattag aacaatttga taatgctgtt
    11761 attttattta tgggtgcagg tgatattcaa aaa
//
LOCUS       DAMQYT010000066        11738 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMQYT010000066 DAMQYT010000000
VERSION     DAMQYT010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11738
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.066"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            360..3065
                     /gene="acnA"
                     /locus_tag="PGA33_001357"
     CDS             360..3065
                     /gene="acnA"
                     /locus_tag="PGA33_001357"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDG6473176.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            3245..3712
                     /locus_tag="PGA33_001358"
     CDS             3245..3712
                     /locus_tag="PGA33_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDG6473177.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDGIDSL"
     gene            complement(3919..4215)
                     /locus_tag="PGA33_001359"
     CDS             complement(3919..4215)
                     /locus_tag="PGA33_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HDG6473178.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(4591..5199)
                     /gene="plsY"
                     /locus_tag="PGA33_001360"
     CDS             complement(4591..5199)
                     /gene="plsY"
                     /locus_tag="PGA33_001360"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDG6473179.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLQVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5405..7396
                     /gene="parE"
                     /locus_tag="PGA33_001361"
     CDS             5405..7396
                     /gene="parE"
                     /locus_tag="PGA33_001361"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDG6473180.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7396..9798
                     /gene="parC"
                     /locus_tag="PGA33_001362"
     CDS             7396..9798
                     /gene="parC"
                     /locus_tag="PGA33_001362"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDG6473181.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDSSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            10048..11508
                     /locus_tag="PGA33_001363"
     CDS             10048..11508
                     /locus_tag="PGA33_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDG6473182.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
BASE COUNT         4103 a         1689 c         2238 g         3708 t
ORIGIN      
        1 aagttggggt gggacgacga aataaatttt gcgaaaatat catttctgtc ccactcccat
       61 caaaagaatg acattgaaaa aaatattaaa gtttggtgcc ataattttct ttctgctcaa
      121 tgaaataata cgtttaaaaa ttgaattgaa tcattcgcga aattgataat tattctcaat
      181 taataaaaaa tacttattac accttacctg tatgataagt tttgcttata tactctgatt
      241 aaaaagtcaa aacctaaatt aagcgttttc taagcatgat gagttgttta tcaataaata
      301 taataataca atttaatcaa ggcatataaa tataaaaatg tatcaagggg gatcattaaa
      361 tggctgcaaa ttttaaagag caatcaaaaa aacattttga cttgaatggc caaagttata
      421 cttactatga tttaaaagct gtagaagagc aaggtattac taaagtttcc aatttacctt
      481 attcaattcg tgttttgtta gaatctttac ttcgtcaaga agatgatttt gtaattacag
      541 acgatcatat taaagcttta agtcagtttg gaaaagatgg aaatgaaggc gaggtgccat
      601 ttaaaccttc tcgtgttatt ttacaagatt tcacaggtgt accagccgta gttgatttag
      661 cttctttacg taaagcaatg gatgacgttg ggggagatat tactaaaatt aatccagaag
      721 taccggtgga tttagttatt gaccactcag ttcaagtgga tagctatgca aatccagaag
      781 ctcttgaacg taatatgaaa ttagaatttg aacgtaacta tgaacgttat cagtttttaa
      841 attgggcaac gaaagcattt gataattaca atgcagttcc tcctgcaact ggaatagttc
      901 accaagttaa cttagaatat ttagcaagtg ttgtacatgt tcgtgatgta gatggtgaaa
      961 aaactgcatt tccagataca ttagttggta ctgattcaca tacaacaatg ataaatggta
     1021 ttggcgtact aggatggggt gttggtggta ttgaagctga agctggaatg cttggacaac
     1081 cttcttattt cccaattcca gaggttattg gtgtacgact agtaaattca ttaccacaag
     1141 gcgcaacagc aactgattta gcgttaagag taactcaaga gctacgtaaa aaaggtgttg
     1201 ttggtaaatt tgtggagttc tttggtccag gtgtacaaca tttaccacta gcagaccgtg
     1261 ctacaattgc aaacatggca ccagagtatg gagcaacttg cggattcttc ccagttgatg
     1321 atgaatctct taaatatatg aagttaactg gtagatcaga cgaacatatc gcgctagtaa
     1381 aagaatattt gaaacaaaac catatgttct ttgatgttga gaaagaagat cctaattata
     1441 cagatgttat cgaattggat ttatcaacag ttgaagcatc gctttcagga ccaaaacgtc
     1501 ctcaagattt aattttctta agtgatatga aatcatcatt tgaaaattct gtaacagctc
     1561 cagcaggcaa ccaaggacac ggtttagata aaagtgaatt tgataagaaa gctgaaatta
     1621 actttaaaga tggatcaaaa gctacaatga aaacaggtga tattgcaata gcagcaatta
     1681 catcatgtac aaatacatct aacccttatg taatgttagg tgcaggttta gttgctaaaa
     1741 aagcagttga aaaaggcttg aaagttcctg aatacgttaa aacttctcta gcaccaggat
     1801 caaaagttgt taccggatat ttaagagatg ctggcttaca accttattta gatgatttag
     1861 gcttcaactt ggttggttat ggatgtacaa cttgtatcgg taattcaggt cctttattac
     1921 cagaaattga aaaagcgatt gctgatgagg acctattagt gacatctgta ttatctggta
     1981 accgtaactt tgaaggtcgt atccatcctc ttgttaaagc caattaccta gcttcaccac
     2041 agttagttgt tgcttatgca ttagctggaa cggttgatat tgatttacaa aatgaaccta
     2101 ttggtaaagg taatgacggt gaagatgtat atttgaaaga tatttggcca tcaattaaag
     2161 aagtttcaga taccgttgat agtgttgtaa cacctgaatt atttattgaa gaatataata
     2221 acgtatacaa taacaacgaa ttatggaatg agattgatgt aactgatcaa cctctatatg
     2281 actttgatcc taattcaaca tacattcaaa atccatcatt cttccaagga ttatctaaag
     2341 aaccgggtac gattgttcca ttaaatggtt tacgtgttat gggtaaattc ggtgattctg
     2401 tgacaactga ccacatctct ccagcaggtg caattggtaa agatacgcca gctggtaaat
     2461 atttacaaga tcatcaagtg cctattcgtg aatttaattc atatggttca agacgtggta
     2521 atcacgaagt aatggttcga ggtacgtttg ctaatatacg tattaaaaac caattagcgc
     2581 caggtactga aggtggtttt acaacttatt ggccaacaaa tgaagtaatg cctatctttg
     2641 atgctgcaat gaaatataaa gaagatggta caggtttagt tgtattagct ggtaacgatt
     2701 atggtatggg ttcatctcgt gactgggcag caaaaggtac aaacttatta ggtgttaaaa
     2761 cagttattgc acaaagttat gaacgtatcc atcgttcaaa tttagttatg atgggtgtat
     2821 taccattaga gtttaaaaaa ggtgaatcag ctgattctct tggtctagat ggtacagaag
     2881 aaatttctgt taatattgat gaaaatgttc aaccacatga ctacgtcaaa gttactgcta
     2941 agaagcaaga tggtgatttg gtagaatttg acgctatggt tcgttttgac tcacttgttg
     3001 aaatggatta ctatcgtcac ggtggaattt tacaaatggt tttaagaaat aaattagcgc
     3061 aataaaaaat agatatcaca gtaaaatttt aatcggtatt tgaggcactt tgatagtgct
     3121 caaaaatact tctataaact ttatgatata ataggtcggt tcaaaacctt tgtgttcttg
     3181 gccggcctct tcttgtattt tatgttagaa tgagatataa agttaaactg aaagaaggct
     3241 taaaatgata tatagtatta cagaaataga agcgcgttat gctgaaactg ataagatggg
     3301 tgtaatttat cacgggaatt atgcaacttg gtttgaagtt gcgcggttgg attatatatc
     3361 gaagttaggt tttagttatg ctgatatgga aaaacaagga atcatttcac ctgtgactga
     3421 cctcaatgtc aattataaaa agtctatttt ttatccagaa aaagttaaag ttaaaacttg
     3481 ggttgaaaaa tattcgagat tacgttcagt gtataaatat gagattttta atgaaaaagg
     3541 tgaacttgca actacaggtt ccacagaatt gatttgcatt aaagaagata cttttaagcc
     3601 tatacggttg gatcgttatt tcccagattg gcatgaagct tatagtaaag tgcaaacgct
     3661 caataatgaa gggaaaacag tagagataat ggatggtatt gattctttat aagttactgt
     3721 aaatgtttgg agaggcaata ttaacaatta atctataaaa aatagccaat acgttaacat
     3781 taatgtaatt agcgtgaaga taagctaata tatagcaaaa acgaacttgt tcatacaaca
     3841 tcagatgtgc taaaccaaag tacaacttcc gaggttcagg acacatcaga atgtgtaaac
     3901 aagttcgttt tattttagtt attttgtgga ataagaaatt tcatcttcgt gatcaactac
     3961 atttacaata atgtggtcat cttcaaagta ccacaaatct ttttccgcta cgacaacatt
     4021 taaatcgtca tattgttgtt catagccaat atcaacatct tcctttggtt caactgtaaa
     4081 agcaggacta aatccttgct tgagttggaa ttcgccacca tatcttacaa aaaacacgag
     4141 cactttatta ttttcaggca actcaagttc atttttaaac caagttactg ctgcatcagt
     4201 aagttctatt tgcataacaa tgacctccta tatttattac agaatgggag tgggccaact
     4261 tgtattattt gtggaatttc ttatcgaaat tctctgtgtt ggagcccacc ccaacctgct
     4321 cattattgta agctgaattt ccataagctt ctatgttggg acccccgacc agaattgaaa
     4381 aaacctagtt ataagcgcat tttgttcggt taactacagc taatataaca ttgtggagcc
     4441 tatgacattg atttatgtct cggtctcaaa tgttcttgta aaaaaactaa gtatagttga
     4501 attttaagtt aaaaaaattg tttttgcaaa aataaaataa tccatcaact gcaaaaaaat
     4561 atgcaatgat ggactaatgt caaaaaattg ttacatccat tttattttag gttcttcgcc
     4621 tctaaaaatc cttgcgatat tagagcgatg tctaattatc aatatgattg aaactaagaa
     4681 actaacgact aataaaatat agtcttgaat gataagcgag ccaatcacac agcaaattgc
     4741 tgcaacgata cttgctaaag aaacatattt aaaaatcttc aatacaataa agaagataat
     4801 tgcaagtatt agtaaaagta tcggattgac tcccaagacg acacctgcac tagttgcaac
     4861 agctttgcca ccttggaatt ttaaataaac aggataaacg tgtccaagta tagcgaataa
     4921 gccaacaatt aaaccatttg taaaaaaagt actaataggg ccatctgcgt gaacttgtaa
     4981 ccataaaggg aagaaaacag ttatgaaccc tttgaaaata tctagaaatg ttaccaagaa
     5041 tcctgcagga cgacctaata ctctaaagct attagtagcg ccagtattac cactaccaaa
     5101 ttgtctaata tcttttttga aaaataattt tccaattacg aatccacttg ggaaagcgcc
     5161 gataagataa cttagtagta acatgacgat tatcatcata aatattacac atcctttaat
     5221 atcttaggac tattttatca tattcattta aattacggct aaaaatttta aaaacgggga
     5281 ttaatatatg gaattaagct atgaaagtta attgatactt gcattttacg ctgatttata
     5341 taagaataac tattgtatag ttttaaaaac gaacgtacgt ttgcaggagg cgaaatcatt
     5401 ggcaatgaat aaacaaaata attattcaga tgattcaata caggttttag aggggttaga
     5461 agcagttcgt aaaagacctg gtatgtatat tggatcaact gataaacggg gattacatca
     5521 tctagtatat gaaattgtcg ataactccgt cgatgaagta ttgaatggtt acggtaacga
     5581 aatagatgta acaattaata aagatggtag tatttctata gaagataatg gacgtggtat
     5641 gccaacaggt atacataaat caggtaaacc gacagtcgaa gttatcttta ctgttttaca
     5701 tgcaggaggt aaatttggac aaggcggcta taaaacttca ggtggtcttc acggtgttgg
     5761 tgcttcagtt gtaaatgcat tgagtgaatg gcttgaagtt gaaatccatc gagatggtaa
     5821 tatatatcat caaagtttta aaaacggtgg ttcgccatct tctggtttag tgaaaaaagg
     5881 taaaactaag aaaacaggta ccaaagtaac atttaaacct gatgacacaa tttttaaagc
     5941 atctacatca tttaattttg atgttttaag tgaacgacta caagagtctg cgttcttatt
     6001 gaaaaattta aaaataacgc ttaatgattt acgcagtggt aaagagcgtc aagagcatta
     6061 ccattatgaa gaaggaatca aagagtttgt tagttatgtc aatgaaggaa aagaagtttt
     6121 gcatgacgtg gctacatttt caggtgaagc aaatggtata gaggtagacg tagctttcca
     6181 atataatgat caatattcag aaagtatttt aagttttgta aataatgtac gtactaaaga
     6241 tggtggtaca catgaagttg gttttaaaac agcaatgaca cgtgtattta atgattatgc
     6301 acgtcgtatt aatgaactta aaacaaaaga taaaaactta gatggtaatg atattcgtga
     6361 aggtttaaca gctgttgtgt ctgttcgtat tccagaagaa ttattgcaat ttgaaggaca
     6421 aacgaaatct aaattgggta cttctgaagc tagaagtgct gttgattcag ttgttgcaga
     6481 caaattgcca ttctatttag aagaaaaagg acaattgtct aaatcacttg tgaaaaaagc
     6541 gattaaagca caacaagcaa gggaagctgc acgtaaagct cgtgaagatg ctcgttcagg
     6601 taagaaaaac aagcgtaaag acactttgct atctggtaaa ttaacacctg cacaaagtaa
     6661 aaacactgaa aaaaatgaat tgtatttagt cgaaggtgat tctgcgggag gttcagcaaa
     6721 acttggacga gaccgcaaat tccaagcgat attaccatta cgtggtaagg taattaatac
     6781 agagaaagca cgtctagaag atatttttaa aaatgaagaa attaatacaa ttatccacac
     6841 aatcggggca ggcgttggta ctgactttaa aattgaagat agtaattata atcgtgtaat
     6901 tattatgact gatgctgata ctgatggtgc gcatattcaa gtgctattgt taacattctt
     6961 cttcaaatat atgaaaccgc ttgttcaagc aggtcgtgta tttattgctt tacctccact
     7021 ttataaattg gaaaaaggta aaggcaaaac aaagcgagtt gaatacgctt ggacagacga
     7081 agagcttaat aaattgcaaa aagaacttgg taaaggcttc acgttacaac gttacaaagg
     7141 tttgggtgaa atgaaccctg aacaattatg ggaaacgacg atgaacccag aaacacgaac
     7201 tttaattcgt gtacaagttg aagatgaagt gcgttcatct aaacgtgtaa caacattaat
     7261 gggtgacaaa gtacaaccta gacgtgaatg gattgaaaag catgttgagt ttggtatgca
     7321 agaggaccaa agtattttag ataattctga agtacaagtg cttgaaaatg atcaatttga
     7381 tgaggaggaa atctagtgag tgaaataatt caagatttat cacttgaaga tgttttaggt
     7441 gatcgctttg gaagatatag taaatatatt attcaagagc gtgcattgcc agatgttcgt
     7501 gatggtttaa aaccagtaca acgtcgtatt ttatatgcaa tgtattcaag tggtaataca
     7561 cacgataaaa atttccgtaa aagtgcgaaa acagtcggtg atgttattgg tcaatatcat
     7621 ccacatggag actcctcagt gtacgaagca atggtccgtt taagtcaaga ctggaagtta
     7681 cgacatgtct taatagaaat gcatggtaat aatggtagta tcgataatga tccgccagcg
     7741 gcaatgcgtt acactgaagc taagttaagc ttactagctg aagagttatt acgtgatatt
     7801 aataaagaga cagtttcttt cattccaaac tatgatgata cgacactcga accaatggta
     7861 ttgccatcaa gatttcctaa cttactagtg aatggttcta caggtatatc tgcaggttac
     7921 gcgacagata taccaccaca taatttagct gaagtgattc aagcaacact taaatatatt
     7981 gataatccgg atattacagt caatcaatta atgaaatata ttaaaggtcc tgattttcca
     8041 actggtggta ttattcaagg tattgatggt attaaaaaag cttatgaatc aggtaaaggt
     8101 agaattatag ttcgttctaa agttgaagaa gaaactttac gcaatggacg taaacagtta
     8161 attattactg aaattccata tgaagtgaac aaaagtagct tagtaaaacg tatcgatgaa
     8221 ttacgtgctg acaaaaaagt cgatggtatc gttgaagtac gtgatgaaac tgatagaact
     8281 ggtttacgaa tagcaattga attgaaaaaa gatgtgaaca gtgaatcaat caaaaattat
     8341 ctttataaaa actctgattt acagatttca tataatttca acatggtcgc tattagtgat
     8401 ggtcgtccaa aattgatggg tattcgtcaa attatagata gttatttgaa tcaccaaatt
     8461 gaggttgttg caaatagaac gaagtttgaa ttagataatg cagaaaaacg tatgcatatc
     8521 gttgaaggtt tgattaaagc gttgtcaatt ttagataaag taatcgaatt gattcgtagc
     8581 tctaaaaaca agcgtgacgc taaagaaaac cttatcgaag tatacgagtt cacagaagaa
     8641 caggctgaag caattgtaat gttacagtta tatcgtttaa caaatactga catagttgcg
     8701 cttgaaggtg aacataaaga acttgaagca ttaatcaaac aattacgtca tattcttgat
     8761 aaccatgatg cattattgaa tgtcataaaa gaagaattga atgaaattaa aaagaaattc
     8821 aaatctgaac gactgtcttt aattgaagca gaaattgaag aaattaaaat tgacaaagaa
     8881 gttatggtgc ctagtgaaga agttatttta agtatgacac gtcatggata tattaaacgt
     8941 acttctattc gtagctttaa tgctagcggt gttgaagata ttggtttaaa agatggtgac
     9001 agtttactta aacatcaaga agtaaatacg caagataccg tactagtatt tacaaataaa
     9061 ggtcgttatc tatttatacc ggttcataaa ttagcagata ttcgttggaa agaattggga
     9121 caacatgtat cacaaatagt tcctatcgaa gaagatgaag tggttattaa tgtctttaat
     9181 gaaaaggact ttaatacaga tgcattttat gtttttgcga ctcaaaatgg catgattaag
     9241 aaaagtacag tgcctctatt taaaacaacg cgttttaata aacctttaat tgctactaaa
     9301 gttaaagaaa atgatgattt gattagtgtt atgcgctttg aaaaagatca attaattacc
     9361 gtcattacta ataaaggtat gtcattaacg tataatacaa gtgaactatc agataccgga
     9421 ttaagggcag ctggtgttaa atcaataaat cttaaagctg aagatttcgt tgttatgaca
     9481 gaaggtgttt ctgaaaatga tactatattg atggccacac aacgcggctc gttaaaacgt
     9541 attagtttta aaatcttaca agttgctaaa agagcacaac gtggaataac tttattaaaa
     9601 gaattaaaga aaaatccaca tcgtattgta gctgcacatg tagtgacagg tgaacatagt
     9661 caatatacat tatattcaaa atcaaatgaa gaacatggtt taattaatga tattcataaa
     9721 tctgaacaat atacaaatgg ctcattcatt gtagatacag atgattttgg tgaagtaata
     9781 gacatgtata ttagctaaaa actatatgca atcacgaaat taaatgataa aatacagtaa
     9841 tgttaaattt tgactaaatt caagggattt atattaaatg ctgaccaagt acttatcgtt
     9901 aaattagcga tatactttga ccaaactaat ataaatctct tgtattgtat aagaaatgat
     9961 gatgtatctc tatatttact gaaggttcaa ttcggtctaa ttttatagaa ggtacatgat
    10021 tcatgtagag gatgcgaggg gtttattttg aaagatttcg atagtttaat acctggatgg
    10081 tttaaagaat ttgtccatgt tggtaccgat ttaatatggt ctcaatattt aattggtcta
    10141 ttattgacag ctggattctt ctttacgatt agttctaaat tcgttcaatt acgaatgtta
    10201 cctgaaatgt ttagagcttt agtagaacgt ccagaaactt tagaagatgg taagaagggt
    10261 atttcgccat tccaagcatt tgcgattagt gctggttcga gagttggtac tggtaatatt
    10321 gctggtgttg cgactgcgat tgttttaggc ggtccaggtg cagtattttg gatgtgggtt
    10381 attgcattta taggtgcagc gagtgcattt atagaagcga ctttggctca ggtttataaa
    10441 gtacatgata aagatggtgg attccgtggt ggtccagctt actatattac taaaggttta
    10501 aatcaaaaat ggctaggtat cgtatttgcg attttaatta caattacatt tgcatttgta
    10561 tttaacacag tgcaatctaa tacaattgcg gagtcgttaa atacgcaata taatattagt
    10621 ccagtaatca caggtattat tttagcaatc gtaacagcta ttattatatt tggtggtgta
    10681 cgtagtattg ctacgttatc ttcgttaatt gtaccgatta tggctatcat ttacattggt
    10741 atggttttag taatattgct atttaattta gatcaaattg ttcctatgat aggtacgatt
    10801 attaaaagtg catttggtat cgaacaagta actggtggcg ctgtaggtgc tgcggttctt
    10861 caaggtatca aacgtggttt attctctaac gaagctggta tgggttctgc gccgaatgca
    10921 gcggcaactg ctgccgtacc acaccctgtt aagcaaggtt taatccaatc attaggtgtg
    10981 ttctttgata caatgttggt ttgtacagca actgcaatca tgattttact atattcagga
    11041 ctgaaatttg gtgataacgc acctcaaggt gttgcagtta ctcaatcagc acttaatgag
    11101 catttaggtt ctgctggagg tattttctta acaatagcag ttacactgtt tgcattttca
    11161 tctgttgtag gtaattacta ttacggtcaa tctaatattg aatttttatc aacaaaccgt
    11221 gtaatattat ttatctttag atgtcttgtt gtagtacttg tctttgtcgg tgcagttgta
    11281 aaaacagaaa cagtatggaa tacggcagac ttatttatgg gcttaatggc aattgtaaac
    11341 attatttcca ttataggact gtccaatgta gcttttgcat tgatgaaaga ttatcaaaag
    11401 cagaaaaaag aaggcaagaa ccctgtcttt aaacctgaaa acttagaaat taacttattt
    11461 ggaattagtg cttggggcgc taacaaatat aagaactctg ataaataaat atttttaatg
    11521 taacgattca ttatataaaa atcaaataaa ccctgaggtt ttcaaattaa agcgaaaatc
    11581 tcagggtttt aaatttgtac tcaattatca tctgtatcat tacactagtt aatctaagat
    11641 ttgattatat attaatatag taaggttgag atttatttcc tccattggtg tgcaaagact
    11701 ataccattga taagacatat tgttggatag atgaatat
//
LOCUS       DAMQYT010000067        11633 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMQYT010000067 DAMQYT010000000
VERSION     DAMQYT010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11633
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.067"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..364
                     /locus_tag="PGA33_001364"
     CDS             <1..364
                     /locus_tag="PGA33_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDG6473183.1"
                     /translation="LSLLNQLLLVSLGTFVGVWLVLSLTLLLSSATNSTGVAIAVGIV
                     FYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVEKGFSKSTKLELHELFIGNIA
                     YISIFLILVVFIFKKKNI"
     gene            737..2701
                     /locus_tag="PGA33_001365"
     CDS             737..2701
                     /locus_tag="PGA33_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDG6473184.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQNCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKDLQAQIDILKMLMHDRYLD"
     gene            3045..4124
                     /locus_tag="PGA33_001366"
     CDS             3045..4124
                     /locus_tag="PGA33_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473185.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMVVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(4229..4822)
                     /locus_tag="PGA33_001367"
     CDS             complement(4229..4822)
                     /locus_tag="PGA33_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDG6473186.1"
                     /translation="MEHIFREGQNGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLAFRGQELLTFIKEAAERYDFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFNTRGAQVEEVWVKGHEITETGLTAGQQILGK"
     gene            complement(4844..5812)
                     /locus_tag="PGA33_001368"
     CDS             complement(4844..5812)
                     /locus_tag="PGA33_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDG6473187.1"
                     /translation="MINNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALTYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEVRPFNTKRQHG"
     gene            complement(5890..6324)
                     /locus_tag="PGA33_001369"
     CDS             complement(5890..6324)
                     /locus_tag="PGA33_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDG6473188.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            6551..6835
                     /locus_tag="PGA33_001370"
     CDS             6551..6835
                     /locus_tag="PGA33_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDG6473189.1"
                     /translation="MSNLEIKQGENKFYIGDDENNALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLLKAVVEHARENNLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            7006..7812
                     /locus_tag="PGA33_001371"
     CDS             7006..7812
                     /locus_tag="PGA33_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDG6473190.1"
                     /translation="MAFHDKTATQVTNIVLNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNSQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(7932..8603)
                     /locus_tag="PGA33_001372"
     CDS             complement(7932..8603)
                     /locus_tag="PGA33_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDG6473191.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQEPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            8873..9865
                     /locus_tag="PGA33_001373"
     CDS             8873..9865
                     /locus_tag="PGA33_001373"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDG6473192.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
     gene            complement(10114..10914)
                     /locus_tag="PGA33_001374"
     CDS             complement(10114..10914)
                     /locus_tag="PGA33_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDG6473193.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKLRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYNGNELYNYKSFNRQVFQQVVDYLNMKQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGNDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLKK"
     gene            11235..>11633
                     /locus_tag="PGA33_001375"
     CDS             11235..>11633
                     /locus_tag="PGA33_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDG6473194.1"
                     /translation="MKLNNYSLKVKNKQLVDNCDLNFYLGQINHIVGKNGVGKSLLAK
                     DFLLNNSGNIPKSISQNVTLISSSSNIPNDITKDFLLSLLKSKFENNRQTFDKIYNIL
                     NIEAIPSNVLLKNLSDGQKQKLKLLSFLLED"
BASE COUNT         3775 a         2098 c         1794 g         3966 t
ORIGIN      
        1 attatcttta ttgaatcaat tattattagt tagtttaggc acatttgttg gcgtttggtt
       61 agttttaagc ttaacgttgc tattatcatc tgcaacaaat tcaacgggag tagccattgc
      121 tgtaggtatt gttttttatt ttgcaagttc tattttagca gttattcaaa cggcactttt
      181 agaaaaaata gactggctaa agtggaatcc tattaatatg atgaatatta tgcttcaaac
      241 agttgaaaaa ggctttagta agtcgacaaa attagaactt catgaattgt ttattggtaa
      301 tattgcttat atttctattt tcttaatact tgtagtattt attttcaaga agaaaaatat
      361 ttagtaactt aaagtattaa atgtctaaat acacacatat tccatcgtaa ttcaaaatca
      421 ttttcaaatc ccttcaccca aataatggtg cggggatttt ttcatccaaa ttttggaaat
      481 tccacatttt acatatcgta attttttagg aaactagtga atataacaaa tccctcctct
      541 catttttaaa atagatatat cacttcccca cttttactta actaaactgc aacggttcct
      601 aataccaaaa tcctgccctc tatttttatc aattcaagca tacttattga aaaatgttaa
      661 cgttttcttg ataatcattg taagcgcatt tattttataa actaacgttt gaaatatact
      721 acaggagtga cacgtaatga ctcaaattac tgaaaaagaa ttaaaaaaga agtatttaga
      781 tttactatcc caaaattttg atactccaga aaaacttgca actgaaatta tcaatttaga
      841 atcaatttta gaattaccta aaggtacgga acatttcgtc agcgatttac atggtgaata
      901 tgaagctttc caacacgtat tacgcaacgg ttctgggaac gtgcgagcga aaatcaatga
      961 tattttcaaa gagagacttt caactaagga gcttaatgac ttaactgctc ttgtctacta
     1021 tccagaagac aaattaaaat tgattaaaag tgatttccaa aattgcggtc aacttaatgt
     1081 ctggtatatc acaacaatcg aacatttaat tgagttaatt aaatattgtt cttcaaaata
     1141 tacgcgttca aaactgcgta aagcattgcc aaaacaatac gtttatatta ttgaggaact
     1201 actgtataaa agtaatgaat atcaaaataa aaaatcatat tacgaaacac ttgttaacca
     1261 agtaattgaa cttaaacagg cagatgattt aattattgga ctcgcttatt ccgtacaacg
     1321 cttagtcgtc gatcatttac acgttgtcgg tgatatttat gatcgtggac cacaaccaga
     1381 taaaattatg gatacactga ttaattatca ttccctagat attcaatggg gtaatcatga
     1441 tgtgctttgg gttggagcct atgctgggtc aaaagtatgc ttagcaaact tacttcgaat
     1501 ttgtgcacgt tatgataatt tagatattat cgaagacgct tatggcatta atttaagacc
     1561 actgcttact ttagctgaaa aatactatga cgcagataat cctgctttta agcctaaaaa
     1621 aagacctgac aaacacgaac gtttaactca acgtgaagaa agtcaaatta ctaaaattca
     1681 tcaagctatt gcgatgattc aattcaagtt agaaatacca attattaaac gtcgtccaaa
     1741 tttcgaaatg gaagaacgtc ttgtgcttga aaaggttaat tatgatacaa atgaaattac
     1801 agtttatggt aatacatacc cattgaaaga cacatgtttc caaactgtca atcgtgataa
     1861 tccagcagaa ttactacctg aagaagaaga agtcatgaat aaactattat tgtcattcca
     1921 acaatctgaa aaattacgtc gtcatatgtc tttcttgatg cgtaaaggct ctctttactt
     1981 accatataat ggcaatttac tcattcatgg ttgtattcca gttgatgaaa atggtgagat
     2041 ggaatcattt gaaattgatg gtcatactta cagcggccaa gaattattag atgtgtttga
     2101 gtatcatgtc cgtaaatcat ttgatgaaaa agaaaatact gatgacttat cgacggattt
     2161 agtttggtat ttatggactg ggaaatattc gtcactattt ggtaaacgtg ccatgactac
     2221 gtttgagcga tactttattg cagataaagc ttctcataaa gaagaaaaga atccgtacta
     2281 tcatcttcgt gaagatgtga atatggttcg taaaatgctc agtgatttcg gattaaatcc
     2341 agatgaagga cgcattatta atggtcacac accagtgaaa gaaatcaatg gcgaagatcc
     2401 tatcaaggct gatggaaaga tgcttgtcat tgatggtggc ttttcaaaag cttatcagtc
     2461 aacaactggc attgcaggct atacactatt gtataattca ttcggtatgc aactcgttgc
     2521 acaccaacaa tttaacgcta aagaaaaaat actttccgaa ggtatcgatg aactttctat
     2581 aaagcgcgtc gtagataaag aattacaacg taagaagatt cgtgatacca atattggtaa
     2641 agatcttcaa gctcaaatag atattttgaa aatgttaatg catgatcgtt acttagacta
     2701 aagacgacat ttgatgcatg tatcgtgcac ttatttagtg gtactgtgtt tacgcattgg
     2761 ctacgcaaac acgatgataa ataggcgacc tagctacacc gaaaaaaatc ccctcactat
     2821 tgcgagtagt gaggggattg gtgtatctat gctaattcct gttaactgta ttataacaac
     2881 tttacagcaa aaaaatgcaa aatcaactaa taaattaatg aaatgaccga caccctcgct
     2941 tctactccaa tttaacaaaa tactatatta gtactatatt tttcattaac atgtatgtta
     3001 cattttctat attacttaaa attacgaagg gacattttta aatcatgcgt atcttaaaag
     3061 agtccattat tgtggcattt gcctttgttg gtgttgtcgt tggtgccggc tttgctactg
     3121 gtcaagaaat tttccagttt ttcacaagtc atggcgcata tagcatttca ggcattattg
     3181 taacaggact attgattact ttaggtggaa tggttgtcat gcatacaggt catcatctaa
     3241 agtccagaaa tcattctgat tcaattaact atttcttata cccctctatt gcaagaggtt
     3301 ttgatattat tttaacaatg tttatgttgt ctttagctat tattatgact gcaggtggtg
     3361 cgtcaaccat tcatcaaagt ttcaacttac cgtattggct gagcgcactc atattagtcg
     3421 cctttatttt agcaacactg tttctaaaat tcgatcgttt aattgctgtg cttggcggtg
     3481 ttaccccatt tttaattgcg attgtcatta tgattgcggt ctactatttc acaacaagtc
     3541 atcttgattt tactgccgct aataatgatg ctcagattca taagcagaaa tcattatcac
     3601 ctggatggtg gtttgatgcg attaactatg caagcttgca aattgctgct gccttcagct
     3661 tcttatcagt gatgggtagt aaagttaaat atcgtgactc aacgttatac gggggcttga
     3721 ttggcggttt aatcattaca tttttactca tgatgattaa tctaggttta atttctcaat
     3781 tcgataaaat taaacacgta gatctaccta cattaaaatt agcgacacaa atgtctccgt
     3841 caattggtat tattatgtct gtcattatga tacttgtcat ctacaatact gttgttggat
     3901 taatgtatgc atttgcgtca cgtttcagcg ttccattcag cagacgttac ttcatcatta
     3961 ttattacaat ggctgtcatc acttatatta gtacatttat cggtttcatt tcattaattg
     4021 gaaaagtatt ccctattatg ggattgttcg gtttcatctt actcatacct gtactctata
     4081 aaggtttaat taagcgtatt accggcaaat ctcatatcga ttaaaatatc tatactattg
     4141 taaaatttag tacagcactt caaaatcaaa aaagcgggca gatgaaaagt aaaaatcttt
     4201 tccatctgtc cgcttcttaa tagaactgtt atttcccaag tatttgttga ccagccgtta
     4261 atccagtttc tgtaatttca tggcccttca cccaaacttc ttcgacttgt gccccacgtg
     4321 tattaaacaa gttaatgact tgttcacttg cagctaatgg cacaattgga tcatgtttcc
     4381 ccatagaaag caacacactg acatctgata aatcctttgt tgacgttact tcaactgggt
     4441 ataacggtgc atataacaat gcttttttaa atggtgcttc tgaacgcaac attaagttaa
     4501 tcgctatatt tgatccattt gaaaatccaa caagtactgc tttttcaata tcaaaatcat
     4561 aacgttcagc agcttcttta atgaacgtca acaattcttg tccacgaaat gccaaatctt
     4621 cttcatcata aacaccttca ccaagacgtt tgaaataacg gttcatccca ttttctgaaa
     4681 cttgtcctct aatacttaac aagtgataat tttcattcaa tgcttcgcct aacggtaata
     4741 aatcgaactc atcaccacct gtaccatgca ataatattag tgttggcgca ccattttgtc
     4801 cttctctaaa aatatgttcc atcacaaatc ctcctcattc caattaacca tgttgacgct
     4861 tcgtattaaa aggtctaact tccgattcaa tatattctct tttattttct aaaaatggtg
     4921 gtaaggataa cccttcgcct aatgtttcat aaggttcatc ttccataaat cctggtccat
     4981 ctgttgaaat ttctattaaa atatgcccca cacgtgcata taatgcttca aaatagaaac
     5041 gattaacgat gcctgagtta ttaataccta cctctttata tttcgttgcc cacgcttcta
     5101 ttgcatcatg atctttcaca cgaaatgaca catgatgtac ctcaccataa ccttgacgtg
     5161 ctgctggccc tttatcatct tttattaaga ttacctggcc accattgcct ccttcgccaa
     5221 cttcaagtaa tgcgacatta tcttcatgcg caatagttgt cataccgtaa acagtctcta
     5281 aaatattttt aaagtcgtca aaataactta ctttaatttc aatggggcct aatccataaa
     5341 tcgctttatc tactggaacc ggtccattct tccaaggtac accaggtgct accccttcat
     5401 ttaactcatc tgaaattaat tgatacactt ggccatcgac ttcttcaaat ggcaacactt
     5461 ttttaccaaa taattcttga ataccttcgt gtttaacacc aaactcatca aagcgctgtt
     5521 cataatatgt taatgcgtca tcgttaggca ctctaaaaga cggtcttgta atggaatttg
     5581 ttcctgcctg cccttttgta atatttggaa aatcaaagaa cgtcatgtct gtacctgccg
     5641 aacctacatc atctgcaaaa aaagtatgat acgtataaat atcatcttga ttgactgtct
     5701 ttttaactaa acgcatgcct agtacttctg taaaaaattt ataattacgt tctgcatcat
     5761 ctgtcattgc agtaacatgg tgaataccta gtaattcatg attatttatc attttatata
     5821 ccctctttat atttttagtg catttatctc gaaatcgaga tactttaatg taaattttta
     5881 gtgcacgcct tacacttctg tagattgtgc acttagtttc ttaaacgctt gttgtaagat
     5941 tgttaattca tcctttgtta acacatcaaa cgcttttgtt aatgtctcag catgcttagg
     6001 gaaaatatct gccatttgac tttgaccttt ttcagttaaa caagccatat atacacgttt
     6061 atcatcttta tccttttcac gtgtaatcca acctttgtcc tctaattgac ttacaacata
     6121 tgaaatgctg ctacttgcaa ttaatacgcg gtctctaata cgttgaattg gttgcggacc
     6181 tttattataa agcaactcga gcactgcaaa ttcagtaata tttaagccat atcgctttac
     6241 gtcttctttt gtaatttgct ctaatgtgtc taacgcccta ttcattccga caaaaacatt
     6301 gagagattgt ttcgttcgat ccatatgatc accaactttt ttattttatt tcgatttcga
     6361 gataaattat atagctgaat agatttaatg tcaagatacg tgttttcatc ttcatttcaa
     6421 actgtgctta ataaaaacga ctacatagat gtggcaatgt cgtcacatga ctgtcatgca
     6481 cctttgactt cgtttaagtt aaaatagaat agggaaatac tataatcaat catgatagga
     6541 gttgatatgt atgagtaacc ttgaaatcaa acaaggcgag aacaaattct atattggtga
     6601 tgatgaaaat aatgctttag ctgaaatcac ataccgtttt gtggataata atgaaattaa
     6661 cattgatcat acaggcgtat ctgatgaact tggtggtcaa ggtgttggca aaaaactact
     6721 taaagcagtt gttgaacacg ctcgagaaaa taatttgaaa attattgcct catgttcatt
     6781 tgccaaacat atgttagaaa aagaagattc atatcaagat gtatatcttg gttaaataaa
     6841 ttttgcgatg gtgcaactgt accatcgctt ttttgttcgc atcaatataa aattgcaaaa
     6901 cgactagtta ttttgcattt caaactttat gctatcaatt cattttcttt tctggtatac
     6961 tttttatact aacttataga aaaatagaaa aagaggcgat gcataatggc atttcacgat
     7021 aaaacagcaa cacaagtgac aaacattgta ctgaacgtaa gagacttaga cttaatgaca
     7081 acattttaca aaaacatttt aggactatcc gttaaaagtt ctgacgataa tacaaccgta
     7141 ttatctgttg ggactggtgg ccatactcta acgttacatt tattagaaga cggccgtcag
     7201 acttccccac gtgaagcagg gctttttcat atagcatttt tattaccaac tactgaggat
     7261 ctagctaact tcttatattt cgtggcacaa aaaaatatgg gcatcggcgc tggtgatcat
     7321 ttagtaagtg aagctttata tttcaacgac cccgaaggta atggtattga agtctatcgc
     7381 gatagacctt catcttcatg ggaatggcaa aatggcaaag ttaaaatgga tactttagaa
     7441 gttgatagcc aaaccttatt aacacatcgt actgatgaag gttggcaagg aatgccagca
     7501 aaaggcatga ttggacactt acatttaaaa acacatgatt tagacgcagc atatcaatgt
     7561 tacattgaac aattaggatt ccaacatgtg tctgattttc cacgtgcgct atttatgtca
     7621 acgaatcatt atcaccatca tattgctgct aatacttggc aatccaatca agcaagacaa
     7681 aacaattcac aaagttatgg attaacacat gttgatattt atcaacccga cgcctcaaca
     7741 catacattta cggcaccaga agggtttgac atcacagttc attcaaatac tgaccttgtt
     7801 ccagaaaaat aatcatccat actttattaa tacaattttt tcacgcaaaa gcctgcgatt
     7861 catttatgta atcgcaggct tttttattgc taacttttta attttatata tttagacata
     7921 cggtattcta tttattcttt tggtccaacc cattcaataa catcttcata agcttggcgt
     7981 gttttatttt tcggtggctc ttgttgtctg tagccaaatg cgaccatcac tgataaacca
     8041 aattgctcag tatctaagat ccctttattt gctaaaatgt ctgtcactgt atccagacta
     8101 aaaccttcca tcggacatga atcaatacct aacaatgcgg ctgtcgtcat catattgcct
     8161 aatgcaatat acgtttgttt acttgaccag tcatacaagg cttgatcatt atcagaaata
     8221 tggaaatctg cttggaatgc atcgaatttt tgttcaacag ctggaatcgt ttgtgcctca
     8281 tattttttaa tatctcttaa catatgttgt acatacggtg atcttgacgt tacattttta
     8341 cgcgcaaaaa ttagcacaaa atggctcgct gtatctaatt gttttgctgc gccccaactg
     8401 tgcgctttta attcatcacg taacgcttga tcttgaatca cgacaaactt ccaaggttct
     8461 aacccaagag aacttggaga caatctacct gactctaata ttgtttcaaa atcttctttc
     8521 gaaactttct tttgtggatc gaattgctta gtcgcatgtc tgaaatgaaa tgcatccata
     8581 attgtttgat tcatattgct cattgaacat agcctcccat ttttatattt attgctatac
     8641 aaattatact tcatgtttta aaaagactaa agtaatccgt ttgcaattca ctataacttg
     8701 tgcacatgtt ataagttttc tgcaataaaa cacacttttg aattttggga aatttagtgt
     8761 tataatatat ttgtgatatt tttcacaatt aaattgtggc gtgattttaa tgtgcataat
     8821 ctttaatttc actcatttac ttttcaatct tttaaaagcg aggtttttaa taatgacaaa
     8881 aattatgttc tttggtacgc gtgattatga gaaagagatg gcattaaatt gggggaaaaa
     8941 gaataatgtc gaagtaacta cttctaaaga gctattatca agtgctacag tcgatcaatt
     9001 aaaagattac gatggcgtaa ctacaatgca atttggtaag ttagaaaatg acgtttatcc
     9061 taaattagaa tcttacggta ttaaacaaat tgcacaacgt actgctggat ttgatatgta
     9121 tgatttagat ttagctaaaa aacacaatat tgtgatatct aacgttccta gttattcacc
     9181 tgaaacaatt gcagagtatt ctgtttctat cgccctacaa ttagtgcgtc gcttcccaga
     9241 tattgaacgc cgtgtacaag cacatgattt tacttggcaa gcagaaatca tgtctaaacc
     9301 agttaaaaat atgactgttg caattattgg tacgggtcgt atcggtgctg ctacagctaa
     9361 aatatatgca ggatttggtg ctacaattac agcttatgac gcctatccta ataaagattt
     9421 agacttttta acttataaag atagtgttaa agaagctatt aaagatgccg atattatttc
     9481 tttacatgtt ccagcgaaca aagaaagcta tcatttattc gataaagcaa tgtttgatca
     9541 tgtgaaaaaa ggtgcaatct tagttaacgc agcacgtggt gcagtcatca atacacctga
     9601 tttaatcgct gcagtgaacg atggtacttt gttaggtgct gcgattgata cttatgaaaa
     9661 tgaagcagca tacttcacaa atgactggac taataaagac attgacgata aaacattatt
     9721 agagttaatc gaacatgaaa gaattttagt aacaccacat attgctttct tctctgatga
     9781 agcagtacaa aaccttgttg aaggtggttt aaacgcagca ttatctgtca ttaacactgg
     9841 tacatgtgaa acacgtttaa attaatatat atatttaaaa gcaaaggcgc gccatgtgcc
     9901 tttgcttttt attttgtata tctgtatcaa aatcgatttg attaaaatcc gctttatttt
     9961 atcatctatt caaatgattt tagtgcgctt attttactat ggcattattg cctaacctgt
    10021 ttgagatata ttaatatttg atgattcgta ttcaaagttt tcatttaaca tgtatttagt
    10081 atcatgatag ctgcttcatt gatgatatac tacctacttt ttcaacactt cttgttcgat
    10141 aatcgttaat acaccctgct tatcattact tggagcgaca gcagaggcaa tattgaacaa
    10201 ttcttcatca tgactatttt ccatcacata actatgctta gcgaacgcta acatatcttt
    10261 atcattattc gcatctccga aggccatgag ctctgaagga gacatttccc atttatctaa
    10321 caatcgtttt aatgcctgcc ctttagtcat gtttggcata ataatatcaa tgctatcgtt
    10381 cccacttgag acaagtttaa tatcattgct gaattgcgtt gctacttctt cgtcaacatt
    10441 cggatgcgtc tcacgattaa tattaaatgc tattttgaca taatcatcct caggtaattg
    10501 ctgtagactg tcaatttctt ttaactgatg ataataaaat ctcgtatctt ctttaaacgc
    10561 ttcagaagta tgttttaaaa tatacgcgct tttcaaacca cagatgacga gttgatcaat
    10621 actttgcttc atatttaagt aatcgacaac ctgttgaaac acctgacgat taaagctttt
    10681 ataattatat aactcattgc cattataaat aactgcacca ttttcagaaa taaaatacat
    10741 atctctatcc ccgaaaatag acttcaactt cgcatattga ttgccactcg cagcaataaa
    10801 tgtaatatct ctatttctaa gttcagtaaa aatcgcttca aatctaagtt tatcatatgt
    10861 cttttttgaa tctaaaaacg tcccgtccat atctaccgca atagccttaa ccatgattat
    10921 cctcctcatt gctcttttag tcgtcttcat tatatccatc tcatgacaag caatgaagtt
    10981 tgtcatacca caatgaacct atattcacat tttgaaattg ctttcatata tgcaaaacac
    11041 attacactgg atatagcatt cagaggcgaa cataaatttc agaatattgt aaaaatatca
    11101 cactatattt atcatttaga caaaatatag tgtgatatac taatttataa catctataca
    11161 taatgtgaaa gaatttttaa aaatacaatt aaatatcggt gtaataaatc cctttataca
    11221 taggagttgt atacatgaaa ctaaataact attctttaaa agttaaaaac aaacaactag
    11281 ttgacaattg tgatttaaat ttctatcttg gtcagatcaa tcacattgtt ggtaaaaatg
    11341 gtgtaggaaa atctttatta gctaaagatt tcttactaaa taatagtgga aatatcccta
    11401 agtccatttc tcaaaatgta accttaatat caagttcatc aaatattcct aatgatataa
    11461 caaaagattt tttattatca ttgttaaaat caaaatttga aaacaatcga caaacattcg
    11521 ataagattta taacatacta aacatcgaag caataccgtc taacgtacta ctaaaaaact
    11581 tgagtgatgg tcaaaaacaa aagcttaaat tattaagttt cttattggaa gat
//
LOCUS       DAMQYT010000068        11622 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMQYT010000068 DAMQYT010000000
VERSION     DAMQYT010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11622
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.068"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..825)
                     /locus_tag="PGA33_001376"
     CDS             complement(<1..825)
                     /locus_tag="PGA33_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HDG6473195.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFL"
     gene            complement(825..1439)
                     /gene="ureG"
                     /locus_tag="PGA33_001377"
     CDS             complement(825..1439)
                     /gene="ureG"
                     /locus_tag="PGA33_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HDG6473196.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            complement(1452..2141)
                     /locus_tag="PGA33_001378"
     CDS             complement(1452..2141)
                     /locus_tag="PGA33_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HDG6473197.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEGTRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            complement(2134..2586)
                     /gene="ureE"
                     /locus_tag="PGA33_001379"
     CDS             complement(2134..2586)
                     /gene="ureE"
                     /locus_tag="PGA33_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HDG6473198.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            complement(2599..4314)
                     /gene="ureC"
                     /locus_tag="PGA33_001380"
     CDS             complement(2599..4314)
                     /gene="ureC"
                     /locus_tag="PGA33_001380"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HDG6473199.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            complement(4311..4721)
                     /locus_tag="PGA33_001381"
     CDS             complement(4311..4721)
                     /locus_tag="PGA33_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HDG6473200.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGAENVNKKGGKRS"
     gene            complement(4735..5037)
                     /locus_tag="PGA33_001382"
     CDS             complement(4735..5037)
                     /locus_tag="PGA33_001382"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HDG6473201.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            5297..6202
                     /gene="yut"
                     /locus_tag="PGA33_001383"
     CDS             5297..6202
                     /gene="yut"
                     /locus_tag="PGA33_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HDG6473202.1"
                     /translation="MKIIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYTFARFINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKATLLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            6419..7573
                     /locus_tag="PGA33_001384"
     CDS             6419..7573
                     /locus_tag="PGA33_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDG6473203.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            7787..8695
                     /locus_tag="PGA33_001385"
     CDS             7787..8695
                     /locus_tag="PGA33_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDG6473204.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            9094..10035
                     /locus_tag="PGA33_001386"
     CDS             9094..10035
                     /locus_tag="PGA33_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDG6473205.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            10156..10710
                     /locus_tag="PGA33_001387"
     CDS             10156..10710
                     /locus_tag="PGA33_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDG6473206.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGIK"
     gene            10782..11552
                     /locus_tag="PGA33_001388"
     CDS             10782..11552
                     /locus_tag="PGA33_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6473207.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIGEYAISKNH
                     KPIILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
BASE COUNT         3879 a         1799 c         2094 g         3850 t
ORIGIN      
        1 tagaaaatca agtttacgat cataaatatt ttctgcgata tagctttgga tggttccaag
       61 tattttctct ataatttgtg tgcgataagc aaaaattcta actgcaaaac catgtgtagg
      121 caattgagaa atagcaacac gacaatcgaa tgtattgcta taagaactaa tggtttcata
      181 aactgaatcg attagttttt ggttaacatc ttcgtgtatg aagtaagcag agccataatg
      241 agagtaatgt tccatataac ctatttcatt gatcgattgt ttattaggat tcaataataa
      301 attatcatat gtgactaact catcttcaat ataaatttca tttattaaat gcatatattg
      361 atacttgaag gcttcgccag tttttgaata accaggagtt aaaatatcag tataaaataa
      421 tgaactagaa ttattgagat tgaacgtatt gtgttgataa aatttagcat tttcatatgc
      481 gatgattgga tcagcgacat attctaaata tgcgttatct ttcaaattaa aagtttgata
      541 ctgctcaaca tgattggaag gtgtcttgta tatttttgtt gcaccttgag atgtcaatgt
      601 cactttagcg ttatcttcga cattcacatt catacggtaa cgatctccat ctaaatagcc
      661 accacctaca tttactatat aaaatgtagg aatggtagat tgatttagat aaactggacg
      721 tatcacttta agtgcttttt caaagaaaat atctcttgat actgatcgat tgccatcgaa
      781 aaacactgtt aaatcaagtt gcccagtcca ttgttgttca tccattatga taatcctttg
      841 agtaaagtgt cgcgttcaat ccaatcgata acttcatcca aaccttcgtc ggtttttaag
      901 ttagtaaaag taaatggacg tttaccacga aatactttag tatcttcagc catttgttct
      961 aatgatgcac ctacataggg agctaaatca gttttgttaa ttacaaagaa atctgactta
     1021 atcatacctt gaccaccttt acgtggaatc ttttcacctt gagcaacatc gataatatat
     1081 attgaaaagt caacaagttc tggactaaat gttgctgcta agttatcgcc accagattct
     1141 atgaaaatta gttctatatc gtcatgacgt tctaataatt cgtctattgc tgcaaagttc
     1201 atagatgcat cttcacgaat cgcagtatga ggacatccac cagtttcaac accaatgata
     1261 cgactttcag gtagaactcc tgaatttact aatatctttt cgtcttcttt tgtatatata
     1321 tcatttgtaa taacgccgat actcatttct tttgaaagac gttttacaac tttttcaatt
     1381 aattgtgttt tacctgcacc tacaggacca ccaataccaa ttttaatcgg atttgccaca
     1441 attataacct cctatgatat gaaaattcta acattgacgt tctcatgcgc catttgattt
     1501 agttctaaac caggcgctgt catgccaaaa tctgcttctt ttaattcgaa aatctgcttt
     1561 cttgttcctt ctatataagg aatcatgtga gtaactatct tttgaccagc agtttgtcca
     1621 agtggaatag cacgaacagc attttgagtt aaacttgaaa cattttgata taaatagtaa
     1681 tcaataatcg tttcaatatc tacacctaaa tgatggccta gcatagtaaa acaaatagct
     1741 ggatttaact ttgctttctt atcttgcatt tgttgatgat accaagcaat ccatgggcta
     1801 ttataaagtt ctaaagccaa tttaaccatg cgagtcccca tttgttttgc accaacacgt
     1861 gtttctttag gtaagttttg aacaaacatc agtttatcta tgtgtaatac tttttgtgta
     1921 tcatcatttt ccaatgcatc ataaactaaa cgcatggcta aaccatcaga ataggtaagt
     1981 tgctcttgta aaaacatttt taaccaagca ataaaagtat gatcgtcatg aattatattt
     2041 cgttgaatat atgtttcaag accaaatgaa tgactgaaag cacctgttgg aaactgtgaa
     2101 tcacagaact gaaataatct taagtgtgta tgatcaatca tgagaatgcc ctatatgtct
     2161 gaaagcctta ttaactttac ggtcttctcg aacatatggg atgcctaaac tttttaataa
     2221 atcttcaact aaataatcat attgtactag catttcagtc tctgtaaatt gtgctggcaa
     2281 atgacgattt cctaattgat gggctatatc tcccatttct tgcaatgttc ttggttgaat
     2341 cactaaaaga tcttctgaat taacatccac aataatcata ttatggtcat ctgcgtataa
     2401 aatatctcca tattgtaagt caataggttg ttttaaacga atgcctattt cagtgccatg
     2461 gtctgtaacg actctttgaa tacgtttaac aagatctgaa ttttcaaggt atactttttc
     2521 gacgtgcttt tgtttttctg aatttgacaa attggcaata ttgccttgga tttcttcaac
     2581 aatcattcta tgttcctcct agaataagaa gtatctttga gttaatggta actcagttgc
     2641 tgcattactt gtaatttttt ctccatctac atatacttca tatgtttgtg gatcaacgtc
     2701 taatttaggt gttgcactgt tatttttcat atctgcttta gataattgtc taatattttt
     2761 aactggacgc accatgcgtt ttaaatttaa tgcacgattg ataccatttt cataagcagt
     2821 tttagacacg aatgtcattg acgtacttgt aaggtttccg ccgtattgac catacatttt
     2881 acggtacttc atcggttcag atgtaggtat agaaccattt gcatcgccat ttacggcaga
     2941 gttaattaat ccgcccttta caactaattc aggtttaacc ccaaagaaaa ttgggtccca
     3001 taagacaatg tcagctagtt tgcccggctc gatagatcct acatattcag aaataccatg
     3061 tgtaattgct gggttaattg tatatttagc gatataacgt ttgatgcgat tattatcatt
     3121 atgttcaaaa tcaccatcta aaggaccacg ttgttctttc atgcgatgtg ctacttgcca
     3181 tgttcgtgta attacttcac ctacacggcc cattgcttgt gaatcggaac taatcatact
     3241 gaatacaccc atatcttgca gaacatcttc tgctgcaatc gtttctttac gaatacgtga
     3301 atctgcgaat gcgatatctt caggaatagc cgcatttaaa tggtgagtaa tcattaccat
     3361 atctaaatgt tcatctacag tattatgtgt ataaggcaaa gttggatttg tagatgaagg
     3421 taaaatattt gaaaatgcag cggatttaat taaatcaggc gcatgaccgc caccagcacc
     3481 ttcagtatgg tacatatgaa gtacacggtc tttaacagca gccattgtgt cttccataaa
     3541 tcctgcttca tttaaagtat ctgcatgtaa tgcaatttga acatcaaatt catcagcaac
     3601 atctaatgca tgactcaaag cagatggtgt tgcaccccag tcttcatgta cttttaatcc
     3661 aattgctccg gcattgattt gttcaatgag tgcagttgga tttgttgctt gtcctttacc
     3721 tgtaaaaccg acattaatcg gtaaaccttc ggcagcttct aacattctat gaatatgcca
     3781 tggacctgga gttacagttg ttgctttaga accttctgaa gcaccagtac caccaccaat
     3841 atgagtcgta ataccacttt ctaatgcgac ctctgcttgt tcaggattaa taaaatgaac
     3901 atgagtatca ataccaccag cagtgacgat tttaccttca gcggcaatga tatctgttgt
     3961 tgaacctata ataatgtcga cattatccat tatatctggg ttgccggcat tacctatggc
     4021 gaaaatataa ccatttttaa tgcctatatc agctttaacc actttatcgt aatcgataat
     4081 aacggcatta gaaatgacaa ggtctgcaac gttcacgtca tcacgtgtta cacgaggatt
     4141 ttgcgccata ccgtctctaa tagatttacc accaccaaaa gtagcttctt caccataaac
     4201 cgcatagtct ttttctattt gagcaaatag attcgtatca cctaaacgaa tggaatctcc
     4261 aacagttgga ccgtataagc tcgtatattg attttgcgtc attttaaagc tcatgatctt
     4321 tttcctcctt ttttattcac gttttcagca ccgttatctc cgaatacacc tgcatattca
     4381 tcattttcat cagttgggcg atagacacgt gactcatcga taggaccatt gaccatacca
     4441 cgaaaaccaa aaattttacg tttgccagca tattcaacta attgaacttc ttttttatcc
     4501 ccaggttcaa atcgaacagc tgctccagct ggaatatcta aatgttttcc atatgccatt
     4561 tcacgttcga aatctaatgc tgcatttgct tcataaaaat gaaaatgtga gcccacttga
     4621 attggtcgat ctcctgtatt ttcaacttcg ataactgttt caggatgatg gttattaatt
     4681 tcaacctctg tactttttgt aataatttct cctggtatca tttgactgcc tcctttaaac
     4741 aatagggtga tgtactgtga ttaacttagt accatcgggg aacgtagcct cgatttcgat
     4801 atctgtaatc atgtgttcga caccatccat gacatcttct ttgtttagaa tttgtctacc
     4861 ataactcatt aactctgcaa cggtcttacc atcgcgtgca ccttctaata attcatcgct
     4921 gattaaagct aatgcctcag gatgatttag tttcaaacca cgtgctttac gacgacgtgc
     4981 aacttccgcc gccactacaa tcattaattt gtcttgctct cgttgtgtaa aatgcaaatt
     5041 aaaaccccca atttcatatt agatacaatt tacaaaattt atattaatcc taattgttgt
     5101 gataaacaag taatatacaa agttcaatgt gtaattagaa aattatattt ttagcatatc
     5161 cgatattgaa gcaaacaatc taatcgaaaa caaatagtgg aatatattta tgtaaaaacc
     5221 aaaatagttt ttaatataac ttttcataga atagtagtat attaatgagt aatgattcaa
     5281 aggaaaggtg aaagatttga agataataga tgtgcttttg aaaaatatat ctcaggttgt
     5341 gttaattagt aataaatgga caggattatt tatcttaata ggattatttg tagccgattg
     5401 gacaattgga ttagcggcta ttgtaggtag catcatcgcc tatacttttg cgcgttttat
     5461 aaattatagt gaggcagaga ttaatgatgg gttagctgga tttaatccag tgctaactgc
     5521 cattgcgtta acaatctttt tagataagtc aggattagat attgttataa caatgatagc
     5581 aactttatta acgttaccag ttgctgctgc agtgagagaa gttttaagac catataaagt
     5641 tccgatgctg acgatgcctt ttgtcattgt gacttggttt acaattttac tttcaggaca
     5701 ggttaaattt gtagatacat cgttaaagtt aatgcctcaa aacattgaaa cggttaattt
     5761 tagcaacaat gatagaatac atttcattca gtcattattt gaaggattca gtcaagtatt
     5821 tatcgaagcg agtgtaattg gtggcgtatg tattttaatc ggcatattga tagcatcaag
     5881 aaaagcaaca ctcttagctg ttatagctag tttgttaagc tttatcattg tagctctatt
     5941 aggtggtaat tatgatgata ttaatcaggg attattcggt tataactttg tattaatggc
     6001 aatcgcacta ggatatacat ttaaaacagc gattaaccct tatatttcga cttttttagg
     6061 tgtgttatta acagtagtgg tgcaactagg tacaacaaca ttgcttgaac cgtttggctt
     6121 acctgcatta acattgccat ttattatcgt gacatggatt ttattatttg ctggtattaa
     6181 acatgacaaa gtagatgctt gatagttaaa tcaaacctaa tattgtttga atatcacctt
     6241 aaactataca gcgaattgta tagtttaagg tgtattttta tggataaaat taagtgcata
     6301 cttaatcgaa ttatcagttt tcttttaatt gttcacagca cgttcctttt tatttttaaa
     6361 gtcgagtgtt aaactaggaa taaataattt atattgtgtg ttgtgtgggg tgactaatat
     6421 gaatgatatg gataattcct ttttaataac aacggaaatt caaagaaaat ggattgaaaa
     6481 attcaaagta attagagata catttaaggc taaagctgaa tataatgatc aacatagcca
     6541 atttccatat aaaaatattg aatggttaat taaagaaggt tatggaaaat taacgttacc
     6601 aaaagcatat ggtggtgaag gtgcgaccat agaagacatg gttattttgc aatcattttt
     6661 aggcgaactt gatggtgcca cagcattatc tattggttgg catgtgagtg tcgtaggaca
     6721 aatttatgaa cagaaattat ggtctcaaga tatgttggag caatttgctg ttgaaattaa
     6781 taatggtgca ttagttaata gagcagttag tgaagctgaa atgggtagtc caacaagagg
     6841 gggaagacca agtacacatg ctgttaaagc tgatgatggg tatattttaa atggtgtgaa
     6901 gacatataca tcaatgagta aagcactaac acatattatt gttgctgctt atatagaaga
     6961 attagaaagt gttggttttt tcttagtaga caggaatttg cctggtgttg aaattgccga
     7021 taattgggac gtattaggta tgcgagcgac agaaagtcat gatttaatat taaatgatgt
     7081 aaaagtacca ttaaaacatt tagtggaaac agagaaaagt aaagcgccaa atggttggat
     7141 attgcatata ccaagctgtt atttaggtat agcacaagct gctagaaatt atgctgtaga
     7201 ctttgcaatc caacatagcc ctaatagtat tgaaggaacg attgcaacat tgccaactgt
     7261 gcaacaaaat ttagggaaaa tggaaacgct attgttatgt gcgagacaat ttttatggag
     7321 tacagcaaaa gggtatcaac aatataaaga tgacagtcag ataagaaatc caacaagtgc
     7381 gagtaaagtg atggtaatga atcaagggct tgaagtgatt gatttagcga tgagaatagt
     7441 tggtgctaaa agcttagaaa tgaatcgacc attacaacgt tactatcgtg acatgcgtgc
     7501 aggattacac aatccaccta tggaagatgc agcatataca aatattgcta agagtattac
     7561 agatacattt taaattttaa aaggtaaatc gatcgttgat tggcataaag agatgaaatt
     7621 tgacgttcgg atttaccctt tttttgtggg gatttatagg gatgttaaga tattttgtta
     7681 aattttttgc gaaaataaaa aataaataac actagctatt gtaaatgtat atgcaatctt
     7741 gtataatttt tgaatgattg ataatcattt tcaataggag gaaattatga aaaaactatt
     7801 attaccatta ataattatgt tattagtgtt agctgcgtgt gggaaccaag gtgaaaaaaa
     7861 taacaaagct gaaactaaat cttataaaat ggacgatggc aaaacggtag atattccgaa
     7921 agaccctaaa cgcattgcag tagttgcgcc aacatatgct ggtggactta aaaaattagg
     7981 tgcaaacatt gtagctgtaa atcaacaagt cgatcaaagc aaagtattaa aagataaatt
     8041 taaaggtgtt acaaaaattg gtgatggcga tgtagaaaaa gttgctaaag aaaagccaga
     8101 tttaattatt gtatactcta ctgacaaaga tattaaaaaa tatcaaaaag tagcaccaac
     8161 agtagttgtt gactataata agcataaata tttagaacaa caagaaatgt tagggaaaat
     8221 tgttggtaaa gaagataaag taaaagcttg gaagaaagat tgggaagaaa caactgctaa
     8281 agacggtaaa gaaattaaaa aagcaattgg acaagatgca acagtgtcat tgtttgatga
     8341 atttgataaa aaattataca cttacggcga taactggggt cgtggtggag aagtattata
     8401 tcaagcattt ggtttgaaaa tgcaaccaga acaacaaaag ttaactgcaa aagcaggttg
     8461 ggctgaagtg aaacaagaag aaattgaaaa atatgctggt gattacattg tgagtacaag
     8521 tgaaggtaaa cctacaccag gatacgaatc aacaaacatg tggaagaatt tgaaagctac
     8581 taaagaagga catattgtta aagttgatgc tggtacatac tggtacaacg atccttatac
     8641 attagatttc atgcgtaaag atttaaaaga aaaattaatt aaagctgcaa aataattcag
     8701 ctatataagt tagtgaaatg agagtctgaa acatatcaat cttttgatat tgtattaggc
     8761 tcttattttt atagctagaa agttagatat ttgtattttt ttaaataata agtgccgttg
     8821 ttatcgttca atttaattaa tgatagatta gtattattat agctaaagta gtatacctga
     8881 gaaaatagct caatgtatct ctttattaat aagttatatc ataattattt tagtgcatac
     8941 tttatggaag ggatatcagg gaatggcttt caattaaaga agaggtttaa aaggattaca
     9001 acagaatgtt atgattttgt agaaagatat ataacaacgt tttataaaaa cataatattg
     9061 ttaatggaaa atgaaatgta agggggattt cgagtgacta agaaagttta ttttaaccac
     9121 gatggtggtg tagatgattt agtatctcta tttttattat tacaaatgga aaacgttcaa
     9181 ttgatagggg tcagtacaat tggtgctgat tgttatttag agccatcttt gagcgcatca
     9241 gtaaaaatta ttaatcgttt ttcaaatgaa gatattcaag ttgcgccatc atatgaacga
     9301 ggaaaaaatc catttcctaa agaatggcgt atgcatgcct tttttatgga cgcattgcca
     9361 attttaaatg agccagtcaa acatgttgct tcaaatgtga gcgacaaaga agcctttgaa
     9421 gacattattc aaactttaaa gagacaatca gaaaaagtaa cattattatt tacaggcccg
     9481 cttacagatt tagcaaaagc actacaaaaa gattcatcta tcgttcagta tatagaaaaa
     9541 ttagtttgga tgggtggcac ctttttacca aaaggaaatg ttgaagaacc tgagcatgat
     9601 ggttctgcag aatggaatgc atattgggat ccagaagcgg ttaaaattgt ttttgatagc
     9661 gatatagaga ttgatatggt tgctttagaa agtacgaatc aagtaccgct aacgttagat
     9721 gttagacaaa gatgggcgaa tgaacgccaa tatacgggca ttgatttttt aggagtaagt
     9781 tatgcagcgg taccaccatt aacacacttt ataacaaatt ctacttactt tttatgggat
     9841 gttttaacga ctgcttatat tggtaacaag gacttggttc attcaattga gaaaaaagtc
     9901 gatgtaataa gttatggacc aagtcaaggt aagacatttg agtgtaaaga tgggcgcaaa
     9961 attaatgtca taaatcatgt agataacaac gcattttttg attatataac tgcacttgct
    10021 aaaaaagtaa attaacagct gtgtagaata attaaggttt taatttatat agaacaactt
    10081 attgtaaact tttcatttct taaagtttac aatggtgcta taataatggt catgaaatac
    10141 gaaaggaagt aaaaaatgac aacaaaacag ttagtatata cagctttaat gacagcgatt
    10201 atcgctattt taggattggt accggtaatt ccactaccat tttcttcagt accaattgta
    10261 cttcaaaaca ttggtatttt cttagcaggt gtgattttag gacgtaaata tggcacatta
    10321 agtgttatcg tctttttatt attagtagtt gctggcttgc cattgttatc aggtggtcgc
    10381 ggtggcatcg gtgtattcgc aggtccttca gcagggtttt tactattata tccagttgta
    10441 gcattcatga ttggggcgat tcgagataga ttcatcaatg aaattaattt ctggatttta
    10501 ttcgttggta ttttagtttt tggtgttata gcattagatg ttattggtac attgattatg
    10561 ggcatgatta ttaacatacc atttacgaaa gctatttcaa tttcattagc ttatttgcct
    10621 ggtgatatat taaaagcaat tgtagcaagt ttgattggta cagctttact taatcactcg
    10681 cagtttcgtc aaattatggg aataaaataa tcatatttaa gatagtaaag taattgaata
    10741 agttgctttg aaatttataa aagtgaaagg agtaggtgtc aatggctagt ataagtatgt
    10801 cagatatata ttgtaacggc actatatttg aaaatgacga cgagcagttg atttatttaa
    10861 cgccttcttt tccacaacga tacacaagta acacatggat atataaaaag acgcctaccc
    10921 aagagcgatg gctgaaagac ttagaacgtc aacatcaatt acatacaaat caaggttcaa
    10981 atcattatgc gtttagtttc ccggaaaatg aacaacttga taatcattgg atggctatgt
    11041 ttaaagatat gaattttgaa ctaggtatta tggaattgta tgccatagaa agtgatgcgc
    11101 ttgccaattt gccgcgtaac tctgacgttg aaattgccat cgttgacgag tcgcatatag
    11161 atgcctattt aaaagttgca tatcagttta gtttgccatt tggaaaagac tatgcagatg
    11221 cacatgaaga aatggtaagg gaacattatc aaaaagatgt gattaaacgc ttagtagctt
    11281 atttaaataa tgaacctatt ggcgttgtag atgtcattga aagtgaaaat tacattgaat
    11341 tagatggatt tggtgtatta gaacaatttc ggcaccaagg aattggatct acaattcaat
    11401 cgttgatagg tgaatacgcc atatcaaaaa atcacaaacc aatcatatta gttgcagatg
    11461 gtgaagatac agcaaaagat atgtatgcaa agcaaggtta tgtctatcaa tcgttttgtt
    11521 atcaaatatt aaaagaagat attggaaatt aacgattgat gataggtttg tattagaata
    11581 aaaaagcagt aagctggttt cgagtagaaa tcaacttact gc
//
LOCUS       DAMQYT010000069        11584 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMQYT010000069 DAMQYT010000000
VERSION     DAMQYT010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11584
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.069"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(132..797)
                     /locus_tag="PGA33_001389"
     CDS             complement(132..797)
                     /locus_tag="PGA33_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6473208.1"
                     /translation="MALVVEDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(797..1852)
                     /locus_tag="PGA33_001390"
     CDS             complement(797..1852)
                     /locus_tag="PGA33_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6473209.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            1988..2662
                     /gene="hssR"
                     /locus_tag="PGA33_001391"
     CDS             1988..2662
                     /gene="hssR"
                     /locus_tag="PGA33_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HDG6473210.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            2655..4028
                     /gene="hssS"
                     /locus_tag="PGA33_001392"
     CDS             2655..4028
                     /gene="hssS"
                     /locus_tag="PGA33_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HDG6473211.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTIDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNQDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVNQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSL"
     gene            4191..4634
                     /locus_tag="PGA33_001393"
     CDS             4191..4634
                     /locus_tag="PGA33_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDG6473212.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDNVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            4631..5086
                     /locus_tag="PGA33_001394"
     CDS             4631..5086
                     /locus_tag="PGA33_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HDG6473213.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVVYVLIWIIQFFKNKNYVDTINKQLKQLK"
     gene            complement(5152..6630)
                     /gene="mqo"
                     /locus_tag="PGA33_001395"
     CDS             complement(5152..6630)
                     /gene="mqo"
                     /locus_tag="PGA33_001395"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDG6473214.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSILFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFDNLEQRGVGIQYKQNVLDIKKQKSGV
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(7011..8609)
                     /locus_tag="PGA33_001396"
     CDS             complement(7011..8609)
                     /locus_tag="PGA33_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDG6473215.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTFTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            8926..10599
                     /locus_tag="PGA33_001397"
     CDS             8926..10599
                     /locus_tag="PGA33_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDG6473216.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKSNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            complement(10752..11381)
                     /locus_tag="PGA33_001398"
     CDS             complement(10752..11381)
                     /locus_tag="PGA33_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473217.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDGNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSSNNQANN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKNQALQQLPNFQTALNAANNEANKFGSNNK
                     VYNDYSIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
BASE COUNT         3907 a         2047 c         1650 g         3980 t
ORIGIN      
        1 aatcagcttt tttacattgc ctaagaactt aatgtcccag cctgtttttt gtaaacccaa
       61 atcgtattct gttatctaac ttttatacta ctatgtctta aatcattaac atttagcgtc
      121 tttacaagcc actaatcagt tattttgcca tcttctaatt caatcactcg atctgcatat
      181 tcaaataatc ttcgatcgtg tgtaatcata ataccaatca tttgttgttc tttaatttgt
      241 tgacgtatca tctcaacaac ttttgttgct ctatcggcat ctaaacttgc tgtgggctca
      301 tctgctaaaa tgattttcgg attattcata aatgctctca taatcgcaac acgttgcttt
      361 tcaccgccag ataactgatg cggatataca ttcaagcgat cttctaaacc aatatttttc
      421 aaaagttgta ttgctcttgt tgaactttgt tgtttggtca ttcccgcttc ttgacctacg
      481 agtgtcaatt gctctatcac ttttaaataa ggaactaaat gtgaagattg aaaaataaaa
      541 ccaatatctt ccaatcgtaa atcactagga cgatgctgtt tatcaaacaa tggcgcatca
      601 ttgtaaagca ctgtaccact cgtttgactt aacaatccgc ctaatatcgt tagcaatgtt
      661 gttttcccag aaccagaggc accatttaaa atgacaaatt ccccttgttc cacttcaaaa
      721 ttaatacctt ttaaaacttt tgtttcagac aaaccttctc cgaaattttt gacgatatct
      781 tcaacgacta atgccattat tctgcacctc caattgcttc gataggatcc actttaaata
      841 atttgataaa tgatagtgag gcacctaaaa tcgctactaa tataaatatc cccaccatta
      901 ataaaatatt ttgcgttgtt aagtaaaaag gcatcgttac cggcatcata aatgatagtc
      961 ctactatgat gatcacagca atacctaccc caattattgt tagtgttaaa atttgtaaaa
     1021 ctaacgcact caataaatgt cttgtcttaa taccaattgc tttcaaaatg ccaatttgtg
     1081 atattttttg aatcgtcata acatagaaaa atgcacttag aacgattgct gtaatagcaa
     1141 acaaactaac aatcatcata tttaacggtg cttgctctgc ttgataactc gcaatattac
     1201 ctgttaaatc tttttcactc acaacttgaa cgtcatttat tttattaagc tcatcacgtt
     1261 gttgttgtgt taaatttttc actggataaa atgccgtatt aaccttattc aatgcattaa
     1321 atccgttatc gttcaatagc acaatggaac tatgcgcata cattgtgtcg tttaaaatac
     1381 cagaaactct ataactagtt gaattatttt taaaatgtaa tctatccccg actctaatgt
     1441 tgtctgcagc taacttatca ttaatagcga cctcatgagc atttttagga taatcacctt
     1501 ttttaagttg tatgcgttgt tgtttaacgt cattcattgt aatcacatct tgttctttat
     1561 tagataattt caatgtttga atattcattt tatatggttc ttgatgaatt acctttttaa
     1621 tttgattctg ttgcgtatca ctaagttgcg atttctcaat ttgcggctct ttcatttttt
     1681 gaacaacata ttcatcatta tcaaagtgtt caaataacga aatattctcc ctaccaagcc
     1741 cttgagctaa accactaata aataacacca taatacttaa taaaagaatg attaatgtga
     1801 ttaaaatata acgaaatttg taaaacataa tctcttttat cgctaatttc atatcgattc
     1861 acttctccct atttcttctt tagcgtcagt ataaggttga aatatgaact caatatgaac
     1921 aattgttttt ttacgcaaaa atattttaca tgtataataa atgaaaaaga taagggagtt
     1981 tatagctatg gtgcaatgtc ttgttgtcga tgacgatcct cgaatcctaa attatatagc
     2041 tagccattta caaacagagc acattgatgc atacacacaa ccaagtggtg aagctgcatt
     2101 aaaactttta gaaaaacagc gtgtcgatat tgcagtggta gatattatga tggatggtat
     2161 ggacggcttt caattatgta atacattaaa aaatgattat gatataccag ttattatgtt
     2221 aacagcgcgg gatgcactta gtgacaaaga gcgtgcgttt ataagcggta ctgacgatta
     2281 tgtaaccaaa ccctttgagg ttaaggaact tatttttaga attcgtgctg tattacgtcg
     2341 atataatatc aattcaaatt cagaaatgac tattggcaac ttaacgctaa accaatccta
     2401 tttggaactc caagtatcta ataaaacgat gacgttaccg aacaaggaat ttcaattatt
     2461 atttatgctt gcagcacgtc ctaagcaaat ctttactcgt gaacaaataa tagaaaaaat
     2521 ttggggctat gattatgaag gagatgagcg aacagttgac gttcatatta agcgactacg
     2581 ccaaagatta aaaaaattaa atgccacact tacaattgaa acagtaagag gacaaggcta
     2641 taaggtggag aatcatgttt aaaacactct atgctagaat tgcgatttat tccattacgg
     2701 ttattttatt tagtgcatta ataagttttg tattaacaaa tgtttactat cattataatt
     2761 taaaagcatc taatgacgcg aaaatcatga agacgcttaa agaagcaaga caatatgaac
     2821 aatcagctaa accaacgcac attcaacaat atttcaaaca tttagggcaa atgaattacc
     2881 aaataatgac cattgatcaa aaaggtcata agacatttta tggggagcca tttagagaag
     2941 atactttatc acaaaatgct attaacaacg tattaaataa tcaagattac catggtatta
     3001 aagataaacc attcgcatta ttcgtaactg gatttttcga caatgtaaca gataatactg
     3061 ttggtattaa ctttaagaca aaagacggtt caatagcagt atttatgcgt ccagatattg
     3121 gagaaacatt tagcgagttt agaacattct tagccgtctt gttaatgttg ttattattta
     3181 tctcgatttc attagttatc gcatcaacct attcaatcat tcgtccagta aaaaagttaa
     3241 agctagcgac cgaacgctta attgatggtg attttgaaac acctatcaaa caaacacgca
     3301 aagatgaaat tggaacatta cagtatcact ttaataagat gagagagtca ttgggacaag
     3361 ttgatcaaat gaggcagcat tttgtacaaa atgtatctca tgaaatcaaa acaccattaa
     3421 cacatattca tcatttatta agcgagttac aacaaacttc tgataaaaca ttaagacaac
     3481 aatatataaa tgatatctat accattacaa cgcagctgag cggattaaca actgaattgt
     3541 tgctcttatc cgaattagat aatcaccaac atttattatt cgatgataag attcaagtca
     3601 atcaactcat taaagatatc attcgacatg aacaatttgc tgctgatgaa aagtcgttaa
     3661 tcattttagc tgacctagaa tcaatcaatt tcctaggaaa tcaacgtttg ttacatcaag
     3721 cattgagtaa tttattaata aatgctatta agtatacaga tgttggtggt gccattgaca
     3781 tcgctttgca acatagtcac aacaatatca ttttcacaat aagtaatgat ggttcaccaa
     3841 ttagtccaca agctgaagca cgtttatttg aacgttttta taaagtgagc aaacatgata
     3901 atagcaatgg tttaggtctt gcaattacaa aatccatcat tgaattgcat catggtacaa
     3961 tccaattcac acaaagtaac gagtatgtaa ccacctttac tattacactg ccaaataatt
     4021 cactttaatc tctacctcct gaaaaaacat ttctgttttt atttcaggag gtatctttta
     4081 tattgattag cgctttctag ccacttaaca tttaatttct accattcaac aaaatttaaa
     4141 tatatcgatg gtcatttatg gtttaataat tttgaaggag gcattaacct atgatgaaac
     4201 tcaatttatt tatcaatgca aatgagacgg aatcttatat tgatatacat gcacctaaaa
     4261 tgaatgataa cgttcaaagt attattaatg cagtcaatga tttagacaaa tcacatacat
     4321 tagtcggata catagacaaa gaaatccata tcattaatgt atcagatgtt ataacattcc
     4381 aagttattaa taaaaatgta acagccatta caagcaatca aaaattcaaa ctaaaattac
     4441 gactttatga actagaaaaa cagttaccac aacatttcat tcgcatttcc aaatcagaaa
     4501 tcgtcaacaa gtattacatt gaaaaattat tattagagcc aaatggactt atacgtatgt
     4561 atcttaaaga tgcacactac acctattctt ctagacgtta tttaaaatct ataaaggagc
     4621 gcttatcaat atgaaaaatt tgaaaaactc attatttatt agcttaatca ttggtttgtc
     4681 cctttcactg ttctttagta tgttatttgc agacggcaaa tattatccac tgaacccaca
     4741 atcaactatt ggcatattgt attatacgca tttcacagaa actaccgtaa tgttgatttc
     4801 tatcatttta tggttactca taggcgtcgt ctttttcctt ggagatttta tctttaaata
     4861 cacagattgg agcattacta aagcaactat aatgcatttc ataacaacgt atgtcggatt
     4921 cttgccttta gcaatgcttg ccggttggtt tccactaaca gtgcattatc taatcatatt
     4981 cacaattatc ttcattgtgg tttatgtatt aatttggata attcaattct ttaaaaacaa
     5041 gaactacgta gacactatta acaagcaact gaaacaatta aaatgatttt tattaaccac
     5101 taaaaaacgg taatgtctat cacattaaaa aagacattac cgtttcgttt attatttaac
     5161 ttgtaaatac ttagttactt cttcattaat tttatgatat aaatcctcat gatctgttaa
     5221 gcgataacca aatgacggca ccatttcttt aatctttggt tcccatcctt tgaattcatc
     5281 acgatagcat ctctgtaaaa catcaaacat aatatctaca gctgttgacg caccaggtga
     5341 tgcaccaagt aatgcagcta atgtgccatc atctgacgta ataacttcag taccaaattg
     5401 taagttacct ttagaatcct cagtatcctt gattacttgg acacgttgcc ctgctgtaat
     5461 cacttcccaa tcttcatttt tagcatttgg gaaaaagact cttaaatcat ccatacgctc
     5521 atcattagat aacatcaatt gtgacactaa atacttcgta agactcattt ctttgatacc
     5581 tgcagataac atcgtcacga tattatttgg tttaaccgat ttaattaaat ccatatgtga
     5641 acctgttttt aaaaatttag gtgagaaacc tgcaaatgga ccaaataaca atgaacgctt
     5701 gccgtctaca aaacgtgtat ctaagtgcgg cactgacatt ggtggcgcac ccactgctgc
     5761 ttttccgtac actttagcat gatgacgatc aatcacttct tgatttgtac agcgcaggaa
     5821 taatccactt accgggaaac caccaatatg ttttgattgt ttaatcccag tcttttggag
     5881 taatggtaaa ctcgcaccgc cagcaccaat aaatacaaaa tcagattcat atgttgtcgt
     5941 ttcattagtt tctaaatctt taactttaac tagccatacc ccagatttct gtttcttgat
     6001 gtctaataca ttctgcttat attgaattcc cacaccacgt tgctctaaat tatcaaataa
     6061 ctttgcagtt aacgcaccaa agttaacatc tgtaccagtt tcatcataag ttaaagcaat
     6121 tggttcatca gacttacgac cttcaatcat taacggtacc caagatttca tttcttcttc
     6181 atcttgcgat aatttcattt tttcgaataa aatacttttc tttaacgttg caacacgact
     6241 ttttataaaa gctacattat cttcgccaat gacaaaactc atgtgtggca ccgcttgaat
     6301 aaagcgatct gggttatcta attgtcctgt tttaactaaa tatgcccaaa actgttttga
     6361 aatttggtac tgctcattta tcttaattgc tttactacaa tcaactgtgc catccttacc
     6421 ttcttttgta tagttcaact cgcaaagtgc tgaatgccct gtaccggcat tattccatac
     6481 atttgaactc tcttcgccag gttgtgctaa tttttcaaac actttaatat ttttctcagg
     6541 tgataattct ttaagtaatg ttcctaatgt tgcactcata ataccgccac caattaagat
     6601 gacatctgtt ttgctatgtt gtgttgtcat aacaaataca gtcccccttt ttaaaatgaa
     6661 taatggaacg ttcgagaaat acgacatatt aataccactt taaatcaata agtgtctcaa
     6721 taggttccca tggtgatgct ctattactat ctactatgta tattaatatt ttataataac
     6781 gaaactactt taattatata ctttatgtgc gatagcacaa agacaaaacc acaaacctta
     6841 acagctatca accaatttaa tttgattgct gattactcac tattcatttt aacataaagc
     6901 gctaccatta ttataatgtc aaacttcttt cattaatgat ccataaaaaa aagatgagat
     6961 gttagaaaac atctcacctt ctgtatattt atattttgaa atacgttttt ttataataat
     7021 gacaagatga aagtccaaat acagatgaat attagtaaac atacactgta tttcaatgtc
     7081 atttttaata attctgattc cttaccaact tgttttactg ctgcagttgc aattgcaatt
     7141 gattgtggtg aaatcaattt tgccgctaca ccaccaactg tattagcaga tacaagaagt
     7201 gaaccacttg ttccaatttg ttgtgcaaca gaagcttgaa ttggtgcaaa taatgagttg
     7261 ttatttacaa ctgatcctgt cataaacaca cctatccaac ctaaaattgg tgatagaact
     7321 gggaagacat tacctgcttt agcaatacct tgacccattg ctgcacttaa accaccataa
     7381 gttgtgattt tagaaattgc taagatgaaa caaattgtaa gaactggtaa ccacaactct
     7441 ttaaatgtaa cgccgaataa tctacctgca tctttaaagt taaccttttt agacattaaa
     7501 attgtaataa taatagttaa taaaatagct gtacctgttt gtccaataat atttaaagtc
     7561 aatactaatg gtttatgcgt aacttcgctg attgttccag gtaagttgaa tttaaatact
     7621 aatgatgata aagcaccatt tggtaagaat aagtttttaa agaatggcgc actccaaatc
     7681 attacaatga ctgttaatac aatgaatggg ctccatgcat gtaatactgc ttttgcagaa
     7741 tgtgcttttg caggttcaat ttcttcatct ttattaacac gataaatgtg ttttggttgg
     7801 aattttttag aaaatactgc taatgctaac attgttaata acggtggaat aatatctgct
     7861 aattcaggtc cactgaatac agttaataat ccttgagtaa atgtataagt gattgaaact
     7921 actaaaattg ctggtaatgt ttctttaaca cctctaaaac catcaataat aaagataagt
     7981 aagaatggaa taatgaaatt tatgattgcc aatgttaaag ttgctgattg agaaacacct
     8041 aatactgaaa catctccagg taatttcaac gtttctacaa cacctacagg gataccaatc
     8101 gcaccaaaag caccagacgc tgcatttgcg actaaacata acatcgcagc ttttaatgga
     8161 ttaaatccta attgtgttaa taaaagtgca caaattgcaa ttggtacacc aaatcctgct
     8221 gcaccttcta aaaatgcgtt gaatgcaaat ccaataagta aaacttgaat acgttggtct
     8281 tgtgaaatat ttgtaatact atcttgaatt gttaaaaatt gtccagattc aacagtaatt
     8341 ttgtataaca atactgccat cataacgata tagccaatcg gaatgatacc ttggaagaaa
     8401 ccttcgacta ctgcaccaga agcgatacca actggcaatt tgaaaaatgg tattgcaatt
     8461 agtaatgtta caacaagtgt tgtaatagct gcataaatac ctttcatttt aaaaacagtt
     8521 aaacatagta aaaacagtac aatcggtatt gctgcaatta aagacgataa taataaatta
     8581 tcaaatgggt taaacgtatt tactaacata ggctgactct tctttccata aagtagtagt
     8641 gtaattgctt cattaaaatc atgctctggc tatagcattt tgttatcgat aacattttac
     8701 agaacagtga gattaatgtg atttttttca caattaaaat ataccatttt ttcagactat
     8761 taacaatatc actttcctat ttaattgttt tatttttcat tttaaatctg ttcatcaaaa
     8821 tgaattgtac ttaatgtaat gaagtaactt atactattca tgaattttac ataagaccgt
     8881 gctaaaataa ggaagatact acgatgagga atggaagtta tagcaatgat taaacagata
     8941 aatgtttcta atatgcaaaa gtttgaatct caattaatga aagctcagtc tgaaggttat
     9001 acacatgtcg ttccatatgc caatgagata atgatttatc aatcgatgtt agatgcggtt
     9061 caattatatc ctaagtctat cgtggttgat tatactgtgg atggacaata caaaaatgat
     9121 tgtcactatt tcggtcaatc atcgatcaac attgccgatt gggctcaaaa caataattat
     9181 tatcctaacc tgatatatgc gattcaacag actcttgatt taatacatta ttactctgta
     9241 gaaacgattt tcgatttagc tttattgaca cttctgaaag gtgatttatc aatcgatggt
     9301 catgtcgttt ttgattttaa agcccctata gcaacaagtg cttcaatatg ggaaactatt
     9361 aaaactattg aagattttga tatgatgtct cagttttatt tgaataaaat ggcttatatt
     9421 gatcatcatc ctataccttt tcgcaacctt tttatcgagg attcagaaca acttaattcg
     9481 ccagataatt ggttgtattc taccaagttt atgctaccta aatggttata taaaatagca
     9541 aagcaacgtg ctgataacaa acaattacaa aacttcggcc tgtatacgaa gcaaccaaac
     9601 gttttaaaag atcacatcgt ttttatcggt gatcatcatc aatatatagg taattcaaaa
     9661 tatttattta cttacttcgt taaacataat ccaatgactg cctgttattt tgttactgac
     9721 gatcgtcgcg gaccacattt catatcacct aagtctgaaa aagctgacga attgattaac
     9781 agcgcacgcg tagttatagt tgaaaatgat atacctgaaa cgttacaacc aaacggtact
     9841 ttaattcaat tacatcaagg cacaccaata atgcaattat ttttagatag taaagagcct
     9901 attaaaaata tagaaacacc tttttatcgt gctaaaaggt ataatcgctg gttgcaattc
     9961 gattatgtta tacattctgc tgatgatatt agccactttt atcaaacggc ttttcctagt
    10021 caccaagcaa atgtattggc ttatggtaat cctaaacacc aatatttact acaaaagagt
    10081 aacgaaagta ctactcaaca acagtataag aaatcgttta aaataaatga tcaaaaacca
    10141 gttttattat acgcacctat tggattagtg agtgcgcaac aattaccact atcagatgct
    10201 ttattcaaag cctatcatgt tgtggttcag ggtgttgatg aagcgatctt accagaagag
    10261 gcgcttgttg caccaaagta tcttagtgct caagacttaa ttctaatgtc tgatgtcgtt
    10321 atcactgatt actcaaatat aatatttgat gcgatggcta tagacaaaac agtagcatta
    10381 tacactccaa atcacagtca atacattgag tctcaaggtg ttaacgaaga tatttggcgt
    10441 catctatcaa aaatttggta tacagaccga caattattaa ttaataattt aatttcccag
    10501 gcaataccag tgattaaata tccacaaata caacataaag aacaaccatt agaatctatt
    10561 tcacaactta tattgtctaa gatgacatct aatcaatagt gtttaaattt ctcagtggct
    10621 gtgaatgagg tttaaaagta ctataaaacg taaactttga tactttaaaa tacgcaaaaa
    10681 acggtaaacc ctaattcata ttatagagtt taccgtttta ttttttaact tgcatcatag
    10741 ttatattaac attattgttg gtagtttgga tcagtaacca ttgcttgtcc agtataatca
    10801 accgttacaa ttgaatattt tccatttgca tttgggtctt taaaactaaa cacatactta
    10861 tagttgccat tatgttcttc aatagaataa tcattataca ctttattatt actaccaaat
    10921 ttatttgctt cattattagc cgcatttaaa gctgtttgga aatttggcaa ttgctgtaaa
    10981 gcttgatttt tatttccatt aaacggataa atttgacgtg caaccggcgc ggcattttgt
    11041 ccataatatg gtgcaacgta acttgatttt tgattattat tcgcttggtt attacttgat
    11101 tggttattat ttgtttggtt ttggtcattg tttgttgcat ttgaattaga ttgttgctgg
    11161 ttatcgtttg cactattatc tttattatct ttgtttacgt ctttactatc atctttatta
    11221 tctttcttat ctttagatga atcatttgtt tttttatctt gttgttcagt tttcgcttta
    11281 tcatcttttt ctttattacc gtctttttgt tggtcactat cttgaccaca tgcagctaaa
    11341 aataatgata atgctagtaa ccctgtaact aatcttttca tacatatctc ctcctataat
    11401 tcgatattca ttgaataatc ttgaaataca tatctaccat gtgtatcttt tcatggcttg
    11461 ttaccaaaga cgtcaacatt aatatgatct gaattatcac aatatccata actacaatct
    11521 aaggttcttt ttattatacc ctaatttttg ttcattatta tttaattttt gtgaatttta
    11581 tgtt
//
LOCUS       DAMQYT010000070        11211 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMQYT010000070 DAMQYT010000000
VERSION     DAMQYT010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11211
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.070"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            147..1295
                     /locus_tag="PGA33_001399"
     CDS             147..1295
                     /locus_tag="PGA33_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDG6473218.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKTQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            1295..2518
                     /gene="thiI"
                     /locus_tag="PGA33_001400"
     CDS             1295..2518
                     /gene="thiI"
                     /locus_tag="PGA33_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDG6473219.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAISAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            2562..3332
                     /locus_tag="PGA33_001401"
     CDS             2562..3332
                     /locus_tag="PGA33_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDG6473220.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSTIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAIIFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMAISMIAGSY
                     AGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            3430..3924
                     /gene="tpx"
                     /locus_tag="PGA33_001402"
     CDS             3430..3924
                     /gene="tpx"
                     /locus_tag="PGA33_001402"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDG6473221.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSDASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            4047..4994
                     /locus_tag="PGA33_001403"
     CDS             4047..4994
                     /locus_tag="PGA33_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDG6473222.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKIPSQFQGFMTELNQWMDTNRPKK"
     gene            5082..6284
                     /locus_tag="PGA33_001404"
     CDS             5082..6284
                     /locus_tag="PGA33_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDG6473223.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(6530..7030)
                     /locus_tag="PGA33_001405"
     CDS             complement(6530..7030)
                     /locus_tag="PGA33_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDG6473224.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            7171..8289
                     /gene="ald"
                     /locus_tag="PGA33_001406"
     CDS             7171..8289
                     /gene="ald"
                     /locus_tag="PGA33_001406"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDG6473225.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            8413..8511
                     /locus_tag="PGA33_001407"
     CDS             8413..8511
                     /locus_tag="PGA33_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473226.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(8605..9660)
                     /locus_tag="PGA33_001408"
     CDS             complement(8605..9660)
                     /locus_tag="PGA33_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDG6473227.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTECEVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            9835..10524
                     /locus_tag="PGA33_001409"
     CDS             9835..10524
                     /locus_tag="PGA33_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDG6473228.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(10829..>11211)
                     /locus_tag="PGA33_001410"
     CDS             complement(10829..>11211)
                     /locus_tag="PGA33_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDG6473229.1"
                     /translation="QLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQASIKAGVHLNK
                     LTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEIVGLSNRKAEDK
                     KKFVLGSVSHKVAKRATIPVLIVK"
BASE COUNT         3781 a         1626 c         2072 g         3732 t
ORIGIN      
        1 agctgtttaa atagagtgaa atagtctaat tcttatttaa taagtagaaa taagattatt
       61 ttgcgctata acctttattt tttgtcggat aatagtataa tgcttgagta aaacaatttt
      121 aacgattgat gattaatgaa agagaagtgt ttattttgat atatctagat aatgcggcaa
      181 cgacgaaagc atttgaagaa gtgttagata cttatttaaa agtaaatcaa tcaatgtatt
      241 ataatccgaa tagtccgcat aaagctggtt tgcaggcaaa tcaattacta caacaagcaa
      301 aaacccaaat taatgcaatg attaattcaa aaacaaatta tgatgttgta ttcactagtg
      361 gtgcaactga atccaataat cttgctttaa aaggtattgc ctatcgtaaa tttgatacag
      421 cgaaggaaat aattacatcc gtgttagagc atccgtccgt attagaggtt gtaagatatt
      481 tggaagcaca cgaaggattt aaagttaaat atgttgatgt aaagaaagat ggcagtatta
      541 acttagaaca cttcaaagaa ttaatgtcag acaaagtcgg tttagtaaca tgtatgtatg
      601 taaataatgt aactggacaa atacagccta ttccacaaat ggctaaagtt ataaaaaatt
      661 atcctaaggc acattttcat gtagatgcgg ttcaagcatt cggcaaaatt tcaatggatc
      721 tcaataacat agatagtatt agtttaagtg gacacaagtt taatggttta aaaggacaag
      781 gcgtcttact tgtaaatcac attcaaaatg ttgaaccaac tgtccatggt ggtggtcaag
      841 aatatggcgt tagaagtgga acagttaatt tgccaaatga tattgcaatg gttaaagcga
      901 tgaagatagc taatgaaaac tttgaagcat tgaatgcatt tgttactgag ttaaataatg
      961 acgtccgtca atttttaaat aaatatcatg gagtttatat taattcttca acttcaggtt
     1021 caccattcgt tttaaatatt agttttcctg gcgtaaaagg tgaagtatta gttaatgctt
     1081 tttcaaaata tgacattatg atatctacga caagtgcttg ttcatctaaa cgtaataaat
     1141 taaatgaagt attggctgca atgggattat cagacaaatc tattgaaggt agtataagat
     1201 tatcatttgg ggctactaca actaaagaag atatagcgag gtttaaagaa atatttatca
     1261 tcatttatga ggaaattaag gagttgctaa aataatgaag tatgatcact tgcttgttag
     1321 atacggggag ttaacattaa agggttcaaa tagaaagaaa tttgtaaatc aattaagaaa
     1381 taatgtaaat aagtcattaa aaggacttga tgggtttgtc gttaaaggca aacgagatcg
     1441 tatgtatatt gaacttgaag accatgcaga tataaatgaa ataacatatc gattatcaaa
     1501 aattttcggt attaaatcta ttagtccagt attaaaagta gaaaaaacaa tagaggcaat
     1561 aagtgcagcg gcaattaaat ttgcgcagca atttgaagaa aacagcacat ttaaaattga
     1621 tgtgaagcgt gccgataaaa atttcccaat ggatacgtat gaattacagc gtgaattggg
     1681 tggtgcagta ttgaagcact tcgacaatat ttcagtgaat gtcaaacgtc cagatcatga
     1741 aattcgagtg gaagttagat tagatgcaat ttatatgtat gaagaagttg ttccgggttc
     1801 aggtggatta ccagttggta ctggtggtaa gacgttacta atgctttcag gcggtataga
     1861 ctcaccagtt gctgggatgg aagtgatgag acgtggcgta acaattgaag cgattcattt
     1921 ccatagtcca ccatttacaa gtgatcaagc aaaagaaaaa gttattgaat tgacacgtat
     1981 tttagctgaa cgtgttggac caattaaatt gcatattgta ccatttacag aattgcaaaa
     2041 acaggtaaat aaagttgtac atccaagata tacaatgact tcaacgagac gtatgatgat
     2101 gcgtgttgct gataaattag tacatcaaat aggggcttta gctattgtaa atggtgaaaa
     2161 cctagggcag gtagccagtc aaacacttca tagcatgtat gcaattaata atgtaacttc
     2221 tactcctgta ttacgtcctt tattaactta cgataaagaa gaaattatta ttaaatcgaa
     2281 agaaattggt acatttgaaa catctattca accatttgaa gattgttgta caattttcac
     2341 ccctaaaaat ccagtaaccg aaccaaactt tgataaggta gtccaatatg aaagtgtctt
     2401 tgattttgaa gagatgatta atcgtgctgt tgaaaatatt gaaacacttg aaataactag
     2461 tgattataaa actattaaag aacagcaaac aaaccaatta ataaacgact ttttataaat
     2521 aaaatcctag agtaaattta aacataaggg gatgttaaac tatggatttg aacttaacga
     2581 tgattataat cataatttta tttggtttta tcgcggcgtt tatagattcg gttgtagggg
     2641 gtggcggttt aatttctacg ccagcattat tagcaatcgg tctaccacca tctgtggctt
     2701 taggtacaaa taaattggca agttcgtttg gttctttaac tagtacgata aagtttataa
     2761 ggtccggtaa agtggactta tatgttgttg ccaaattatt tggttttgta tttttggcat
     2821 ctgcatgtgg cgcatatatt gcaacgatgg ttccgtcaca aatattgaaa cctttaatca
     2881 tcattgcact ttcgtcggtg tttatattca cattacttaa aaaagattgg ggcaatacac
     2941 gcacgtttac tcaatttaca tttaagaaag ccataatatt tgcagcactt tttatattaa
     3001 tcggctttta tgatggattt gtaggtgggg gaacaggttc atttatgctt tttgtattgt
     3061 tagtctttgg ttttgatttt ttaagtgcag caggaaatgc taaagttttg aactttgctt
     3121 ctaatatagg tgcgcttgta ttatttatgg tattaggaca agtagattat gtaataggtt
     3181 taattatggc tataagtatg attgctgggt catatgctgg ggctcatttt gctattaaac
     3241 aaggtgttgg ttatgtaaaa gtattattta ttattattac tgctatttta attttaaaaa
     3301 atgcttttga ttatattcaa caatttgtat aaaattaaat ttgcttttga ttaaagcatg
     3361 aaaattgtaa tcaaaccata aattgtcgta tgatgtagtt agaattttaa aatgcaggag
     3421 gtcaagtata tgactgaaat aacattcaaa ggtggaccaa tccacttaaa aggtcaacaa
     3481 attaatgaag gtgattttgc acctgatttt acagtgttag ataatgactt aaatcaagta
     3541 acattagcag attatgctgg taaaaagaaa ttaattagtg tggtaccatc aattgataca
     3601 ggtgtttgtg atcagcagac tcgcaaattc aactctgatg cttctaaaga agaggggatt
     3661 gtgcttacaa tttcagcaga cttaccattc gcacaaaaaa gatggtgcgc ttcagcaggt
     3721 ttagacaatg tcattacatt aagtgaccac cgtgacttat catttggtga aaactatggc
     3781 gttgttatgg aagaacttcg cttattagct cgtgcagtat ttgtattaga tgcagataat
     3841 aaagttgttt ataaagaaat cgttagtgaa ggtactgatt tcccagattt tgatgctgct
     3901 ttagctgcat acaaaaatat ttaatcatta aagagataaa tcttaaaatg tatacatcgt
     3961 gtccatcgtt gtcaacagca ttaaaataga attgttttct atgattgcta agacctatgg
     4021 gcacttttta ttggagaggg acgaatatgg cagaacaaca aacaattatg gaacgcttgt
     4081 ttcatacatt agatgaaaaa gctaaaacat taaataatga aaatggccaa agttttattg
     4141 aaaatcttgg gctagcaatg gaacaagtat ataccaatga aagaggattg ttagaacaat
     4201 cgacgttaca agatcgacgt aaagcattcc aatttgcata tttaagttta atgcaggaag
     4261 aaaagataca agcaaatcat caaattacac cagattcaat tggattgata ctaggatttt
     4321 tagttgagcg ttttatgaac aaccaagaag aattacatat tgttgatatt gcaagtggtg
     4381 ccggtcattt aagtgctact gtaaaagaag tgttacctga aattgcggtt atgcatcatt
     4441 taattgaagt tgatccagtt ttatcacgtg ttagtgtaca tttagcaaac ttcttagaaa
     4501 ttcctttcga tgtgtatcct caagatgcca tcatgccact accattagaa gaagcagata
     4561 tcgttattgg tgattttcca gtaggctatt atccaattga tgaaagaagt aaggagttta
     4621 agctaggttt tgaagaagga catagttatt cacattattt attaatagaa caagcaataa
     4681 atgcattaaa agatgctgga tatgcctttc tagtggtacc aagtaatatt tttacaggtg
     4741 aacatgtaaa acagcttgaa aaatatattg caacagagac agagatgcaa gcatttttaa
     4801 atttaccacc aactttattt aaaaatgaaa aagcgcgaaa atctatatta attttacaaa
     4861 agaaaaaatc gggtgaaaca aagccagttg aagtattatt ggcaaatatt cctgatttca
     4921 aaattccttc acaatttcaa ggatttatga cagagttaaa tcagtggatg gacacaaatc
     4981 gtcctaaaaa ataatctgta atagtatagt cataaaactg tatgaatgat aaaatgaaaa
     5041 atgaaatagt ataaaaagca aacgatggag gatcattact tatgtcaaaa ttaatcttgg
     5101 ctatcaatgc tggtagttca tcattaaaat ttcaattaat tagaatgcct gaagaggaat
     5161 tagtaacaaa aggtttagta gaaagaatcg gattgaagga ttcaattttt acaattgaag
     5221 tcaacgggga aaaagttaaa acagtacaag atatcaagga tcacgttgag gcagttgata
     5281 tcatgttgga tgcttttaaa gcgcacaata ttattaatga tattaacgat atcgatggaa
     5341 caggtcaccg tgttgtacat ggtggtgaaa aattccctga atcagtagct attactgatg
     5401 aggttgaaaa ggaaattgaa gaattaagcg aattagcacc gcttcacaac ccagctaact
     5461 tgatgggtat tcgtgctttc cgtaaattat taccaaatat ccctcatgtg gcaatttttg
     5521 atacagcatt ccatcaaaca atgcctgaaa aagcatatct atatagcttg ccatatcatt
     5581 attataaaga ttatggcatt cgtaagtatg gtttccatgg tacaagccat aaatttgtat
     5641 cacaaagagc ggcagaaatg ttagataagc caattgaaga tttacgtatt atttcatgtc
     5701 acattggtaa tggagcatca attgcagcta tcgatggcgg taaatctatt gatacatcaa
     5761 tgggcttcac accacttgct ggtgtaacaa tgggtactcg ttctggtaat attgaccctg
     5821 ctttaattcc atttattatg gaaaaaacag gtaaaactgc tgaacaagta ttggaaatct
     5881 taaacaaaga atcaggttta ttaggattat caggtacatc tagtgattta cgtgacttat
     5941 cagaagaagc tgaatcaggt aaagcacgtt cacaaatggc tttagatgta tttgcttcta
     6001 aaattcacaa atatattgga tcatatgctg cgagaatgca tggtgttgat gttatcgtat
     6061 ttacagcagg tatcggtgaa aactcagtag aaattcgtgc gaaagttctt gaaggcttag
     6121 aatttatggg tgtttactgg gatcctaaga aaaatgaaaa cttactacgt ggtaaagaag
     6181 gatttattaa ctatcctcat tcaccagtta aagttgtcgt tattccaaca gatgaagaaa
     6241 gtatgattgc ccgtgatgtt atgacattcg gtggtttaaa ataattttta aataaatatt
     6301 aagcgtctat ctccgctaca attatatgta ggaggtagac gctttttgta ttgtttgaaa
     6361 attaactagt gtaatgtctc ttttcacgaa tatcttaaag aattaagtag tcgaagaaga
     6421 ttatgaaaac attattaagt gagttttatt tataaacaat aaaaaaccaa gtgcagaaag
     6481 ttgcacttgg ttcataatga tcaagtagtt atctaatgat catctatatt taattttgat
     6541 atttttcacg taattgagtt gttgcaactt gtggttggaa gtctgctggt aactcttcag
     6601 tacgaacaac taacacgtca catggcgcat gacgaacgat agattctgat acagaaccaa
     6661 caataaatct ttccacggca tttaagcctg atgtaccact cataatcaag tctgcattaa
     6721 tttcatgtgc aagcttttta ggaatgatag atttaggaga gccaaactct agacgcgttt
     6781 ctacatcttt aacaccagcg ttagtagcta cttctttata accatttaat aattcttctg
     6841 caaaatgctt agatttttca gtaaattgag catcataaac ttcataagaa gaatacgttc
     6901 ttgaatcaat tacattcaca attgttaact tcgcatcgtt acgtttagca acaccaactg
     6961 ctctgttaaa tgcccattcc gcttcatgtg aaccgtcaac tgcgattaaa atatttttgt
     7021 aagtaatcat agtaagtgcc tccttgttct ttcttaatta tattttacca caattttcgg
     7081 aaaagtcagt gtaataacct ttacaatttt gtggattttt gattgcgctt tcaaaatgtt
     7141 cgcgttaaga tgattatgga ggtggatggt atgaaaattg gtataccaag ggagattaaa
     7201 aataatgaaa atcgtgttgg tttatcacca agtggtgtgc acgctttagt agaaagtggg
     7261 catactgttt tagtggaaac aaatgcgggt tcaggatcat tctttgaaga tgtagattac
     7321 aaagaagcag gtgctgagat tgttgctgaa caagcaaaag tttgggatgt ggatatggtt
     7381 attaaagtta aagaaccact tgaatctgaa tatccatatt ttaaagaagg gcttgtatta
     7441 ttcacttatc ttcatttagc aaatgaagaa aaattaacac aagctttgat agatagaaaa
     7501 gtaattagta ttgcatatga gactgtgcag ttaccagacc gatctttacc attgttatca
     7561 ccaatgagtg aggtagcagg aagaatgtca gctcaagttg gcgcagagtt cctacaaaaa
     7621 cttaatggtg gtatgggaat tctactaggt ggtgtcccag gagtacctaa gggtaaagta
     7681 actattatcg gtggtggtca agcaggaaca aatgcagcta aaattgcact aggactaggt
     7741 gcagatgtta cgattttaga tgttaatcca aagcgtttac aacaattaga tgatttattc
     7801 ggtggacgtg tacatacaat tatgtcaaat ccgttgaata ttgagttgta tgttaaacaa
     7861 agtgatttag taataggtgc agttttaatt ccaggtgcta aagcgccaag acttgtaaca
     7921 gaagacatga ttaaacaaat gaaaaatggg tcagttatta ttgacattgc tattgatcaa
     7981 ggcggtattt ttgaaacaac tgataaaatt acgacacatg atgatcctac atatattaag
     8041 catggtgtgg ttcattatgc agttgcaaat atgccaggtg cagtaccgcg tacttcgacg
     8101 ttagctttaa ataatgctac gctaccttat gcgctcatgc tagctaataa agggtataga
     8161 gaagcattta aatcaaatca accattatca ttaggtttaa atacttacaa aggtcacgta
     8221 accaataaag gcgttgcaga ggcatttgaa atggaatata aatctgtaga agaagcatta
     8281 caattataat ttgaatttga tgaaaaggga atggattaca gcgtactaaa gaatgacgtt
     8341 atcatctcct tggttattaa aattaaataa tgataaatta aagctagaaa caatgcgaag
     8401 aaatcatcga acatgaatga tattgatttg attcattaca tgttactgat gaacatacct
     8461 gcatatgtgt ttctagcttg tttgttacat attgcattta gacttggata aaaatttatg
     8521 tcttaggcgc tacaatgtta tattggcagt gtttgactga atgagaatgc gcttgtaaca
     8581 agcttttcat ttttatccca ctccttattt ttcgtaatgt gttaatactt catatccttc
     8641 attagtgaca agtatgtcat cttcaattct tacacctgca acaccaggta cataaatacc
     8701 tggttcgatt gtaataacca tgccagcttc taacaaatta gaattagtac ttgaaacatc
     8761 ttgatattca tgttcttgta atcctaggcc atgacctaag cgatgaggga aatattcacc
     8821 ataacctttt tctgaaataa tatttctagc gatatgatcg atatctttta atggtattcc
     8881 aggtttaatt gcttggattg cagatgtttc tgcttctaat acaatattat aaatttcttg
     8941 tgcttctttg ctaggttcac caaatttaat agtacgtgtc atatcgctac aataatgctc
     9001 ataaattaca cctaaatcaa atagtacata ttcattgctt tttaatctgc gatctcctgg
     9061 tgtgccatga ggtgatgcgg catgatctcc aaataaaacc atcgtatcaa aactcatttc
     9121 attgacgcca tattgtttga tagtttgctc aatatggttg actacttcac attcagtcac
     9181 accttctttt aaataagaaa cacctatttc gatacactta tctgctaact cagcagcttt
     9241 acgtattttg ctaatttcat cttcggattt aatatttctc aattgtttga ttgttaaatc
     9301 aacatctccg aatgaattga cattgaaacc agagattaat tgtttttggc gtgctactgt
     9361 taagtgctcg ctttcaatta gtaatttatt gattgtttga ggataaagtg aaaaagggtt
     9421 ttcagtgtct aaatatccaa cgatttcacc tgtgaaaggt gatgctttga cttcttcgac
     9481 ttccattttt ggacaaaata gtacttgttt accatctttc ttaatcaata atgcaaataa
     9541 tctttcatgg ggttcgctac ggtatccagt aaaataatat acattcaacg gtgttgtaat
     9601 ccatgctgca tcagcttgtt gattgttcaa ttcgtctatt atttttgata tttttgtcat
     9661 tttataaaca ctcctcaaca taatattact tccattttat gccaattatt attagatttc
     9721 aaaatcgagc taatacatta actagatttt caatttttaa aatttgagaa tttgttataa
     9781 ttaagtggca aaagggtact aaatttatat agtaattgat aggaggatgc tcaaatgaag
     9841 ttatcatttc atggtcaatc aacgatttat ttagaaggga acaacaaaaa ggttatcgta
     9901 gatccgttta tttcaaataa ccctaagtgt gatttgaata ttgaaacagt gcaggtagac
     9961 tatatcgttt taacgcacgg tcattttgat cattttggtg atgttgtgga actagcaaag
    10021 aaaacaggag caacagtaat tggaagtgca gaaatggctg actatctttc ttcatatcac
    10081 ggtgttgaaa atgttcatgg tatgaatatt ggtggtaaag caaactttga tttcggaagc
    10141 gttaaatttg ttcaagcatt tcatagttca agttttacac atgaaaatgg tattccagtt
    10201 tatttaggta tgccaatggg tatcgtgttt gaagttgaag gaaaaacaat ttatcatact
    10261 ggtgatacag gtctgtttag tgacatgtca cttattgcga aacgtcatcc agttgatgta
    10321 tgttttgtac caattggaga taattttacg atgggtattg atgatgcaag ttatgctatt
    10381 aatgaattta ttaaaccaaa aatttctgta ccgattcatt atgatacatt cccattaatc
    10441 gaacaagatc cacaacaatt taaagatgct gttaatgtag gggacgtgca aattttaaaa
    10501 ccaggtgaat ctgttcagtt ttaaatagaa tatagatttt acactaaaaa acacctcgct
    10561 aatctatgtg gaagttatcg tctgcatatt tttagtgggt gtttttatat tctactgatt
    10621 cagttacgac gtggacgggg gacatcagtg gttctttatc atttctgacc tactctcgta
    10681 attttagttt tatacaatta gacattgatt ataaatttac tacgatataa aaaatccaga
    10741 accacagtca taatcgtttc ttgtatttac tcagtcgttt gagactgcat catgattgaa
    10801 agattatttg tgattctgga taaaaatttt atttaacgat taatacaggg atagtcgcac
    10861 gttttgctac tttgtggctg acacttccaa gtacaaattt ctttttgtct tctgctttac
    10921 ggttacttaa accaacaatt tcgtatttac cgctattagc atgttttaat agttcttctt
    10981 ttgcattacc acgaacaatt atttggtcat aatcaatacc ataatcttct aaagctttgc
    11041 gtgttttttc caatcgcttg cttcgttctt cagtaagttt gtttaaatga acacctgctt
    11101 taattgatgc ttgagcatct tgttcgctga ttgcgtttaa aactgttacg actgtacctt
    11161 cgccagctaa tttagacact tcttttagtg ctttttcatt ttttaactga g
//
LOCUS       DAMQYT010000071        11038 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMQYT010000071 DAMQYT010000000
VERSION     DAMQYT010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11038
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.071"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(65..625)
                     /locus_tag="PGA33_001411"
     CDS             complement(65..625)
                     /locus_tag="PGA33_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDG6473230.1"
                     /translation="MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLQDCPSKPKHN"
     misc_binding    683..984
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1028..3658
                     /locus_tag="PGA33_001412"
     CDS             1028..3658
                     /locus_tag="PGA33_001412"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDG6473231.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     gene            3671..4942
                     /locus_tag="PGA33_001413"
     CDS             3671..4942
                     /locus_tag="PGA33_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDG6473232.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            5212..5919
                     /locus_tag="PGA33_001414"
     CDS             5212..5919
                     /locus_tag="PGA33_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDG6473233.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            5916..6602
                     /gene="radC"
                     /locus_tag="PGA33_001415"
     CDS             5916..6602
                     /gene="radC"
                     /locus_tag="PGA33_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDG6473234.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIKVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            6670..6954
                     /locus_tag="PGA33_001416"
     CDS             6670..6954
                     /locus_tag="PGA33_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473235.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(7071..7268)
                     /locus_tag="PGA33_001417"
     CDS             complement(7071..7268)
                     /locus_tag="PGA33_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473236.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            7428..7901
                     /locus_tag="PGA33_001418"
     CDS             7428..7901
                     /locus_tag="PGA33_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HDG6473237.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKSENAYQ"
     gene            7975..8094
                     /locus_tag="PGA33_001419"
     CDS             7975..8094
                     /locus_tag="PGA33_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473238.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            8294..9136
                     /gene="mreC"
                     /locus_tag="PGA33_001420"
     CDS             8294..9136
                     /gene="mreC"
                     /locus_tag="PGA33_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDG6473239.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            9136..9666
                     /gene="mreD"
                     /locus_tag="PGA33_001421"
     CDS             9136..9666
                     /gene="mreD"
                     /locus_tag="PGA33_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDG6473240.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    9693..9820
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            9833..10141
                     /gene="rplU"
                     /locus_tag="PGA33_001422"
     CDS             9833..10141
                     /gene="rplU"
                     /locus_tag="PGA33_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDG6473241.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            10147..10467
                     /locus_tag="PGA33_001423"
     CDS             10147..10467
                     /locus_tag="PGA33_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDG6473242.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            10479..10763
                     /gene="rpmA"
                     /locus_tag="PGA33_001424"
     CDS             10479..10763
                     /gene="rpmA"
                     /locus_tag="PGA33_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDG6473243.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
BASE COUNT         3895 a         1496 c         2049 g         3598 t
ORIGIN      
        1 aaacgagaca gtctattagt taaaaataga tgatgtctcg tttactttat gatgctatag
       61 caaattaatt atgtttaggc tttgatggac aatcttgtaa atgtgcatca tataaaccgg
      121 ctgcttctaa aaacgaaaat actgttactg gacctaaaaa tttaaacccg tattgtttta
      181 aatctttaga tagttgtgtt gcagtatcat caactgtgat acgatcagaa gcatgttcat
      241 actgcaaatc tttaggctta ccatttacat atgaccataa aaatttacta aaactaccat
      301 atgcttgttc aatttttaaa tacccttgag cttgattaac aattgcttct aattttttac
      361 gatgatgaac gatatttgga aaagtcatta agcggtcgat atcttgagcg gtcatttgtg
      421 ctaccttttc tggttcgaaa tcataaaatg cttcttcata ggcttctttc ttttttaaaa
      481 tagttaacca agatagccca gcatgttgtg attctaatgc taaaagttta aacaatgcct
      541 tgctatcata gagcggttgt ccccatacat gatcatgata gtctaagtag actggatctt
      601 tagtaccaaa tgcgcattca ttcattttat tcccacctat acattgactt attttgtcaa
      661 tcattatact ataagttaag agtttaatat aggatttggg aagagtaagt tcaaattatt
      721 ttaaagagag catatgattt ggtgtgagta tgtaaatgat ttttactgaa ggtagcccac
      781 agagtatctt agctgaacgt gattaatagt aggctaagac gtgtttgagc gttaatcata
      841 atctagagtg cttgaagaga tgaagcattt caaatttcac gatgatagta ttggtatgat
      901 tattccaatt atgttaaaag agtgaatttg atattaatca tccttcttga atataggtgg
      961 taccacggaa tatccgtcct atttgtatat aggatggata tttttatttt tttaggaggt
     1021 atagcaaatg gaaatgaaac caaaatatga tcctcgtgaa gttgaagcgg gacgttatga
     1081 agaatgggta aagaatggtt attttaaacc gtcagaagat aaatcaaaag aaacatatac
     1141 aattgttatc ccgccaccaa atgtaactgg taaattacat ttaggacatg catgggatac
     1201 gactttacaa gatatcatta cacgtatgaa acgtatgcaa ggatacgata cgttatactt
     1261 accaggtatg gatcatgctg gtattgcgac acaggcaaag gtagaagcta aattaaatga
     1321 acaaggaata actagatatg atcttggtcg tgaaaagttt ttagaacagg catgggattg
     1381 gaaagaagag tatgcgtcat ttattcgtgc gcaatgggct aaattaggtc taggtttaga
     1441 ttatagtaga gaacgtttta ctttagatga aggtttaagt aaagcagtta aaaaagtttt
     1501 tgttgattta tacaataaag gaattattta tcgtggcgaa cgtattataa attgggatcc
     1561 taaagcacgt acagctttat ctgatattga agtaatacat gaagatgttc aaggtgcgtt
     1621 ttatcatttt aaatatcctt acgctgatgg tgaaggtttt attgaaattg caacaacaag
     1681 accagaaacg atgttaggtg atacagcgat tgttgttaac cctaatgacg aacgatacaa
     1741 agatgtaatc ggtaaaactg ttatattacc aatcgtagga cgcgaactgc ctattttagc
     1801 agatgagtat gttgatatag acttcggttc tggtgctatg aaagtgacac cagcacatga
     1861 ccctaatgat tttgaaattg gtcaaagaca tcaattagaa aatattatcg ttatggatga
     1921 aaatggtaaa atgaacgaca aagcgggtaa atatgaaggt atggaccgtt ttgattgtcg
     1981 taaacagcta gttaaagatt taaaagaaca agatttagtt atcaagattg aagatcatgt
     2041 tcattctgta ggtcattcag aacgatctgg cgctgttgtt gaaccatatt tatcaacaca
     2101 atggtttgtg cgcatggaag acttagcgaa acgttcatta gataaccaaa aaacagatga
     2161 tcgtattgat ttttatccgc aacgtttcga acatacattt aaccaatgga tggaaaatat
     2221 tagagattgg acgatttcaa gacaattatg gtggggtcat caaattccgg cttggtatca
     2281 taaagaaaca ggcgaaatat atgttggaga agaagcgcca actgatattg aaaattggca
     2341 acaagatgaa gatgtattag atacgtggtt ctcaagtgct ttatggcctt tctctacgtt
     2401 aggttggcct gatttagaaa gtgaagactt taaacgatac tacccaacaa atgccttagt
     2461 tacaggttac gatattatct ttttctgggt agcacgcatg atattccaag gcttagaatt
     2521 tacagatcgt cgtccattta atgatgtatt attacacggt ttagttcgtg ctgaagacgg
     2581 gcgtaagatg agtaaatcat taggtaatgg tgtggatcca atggatgtta ttgacgaata
     2641 cggtgctgat agcttgcgtt acttcttagc aacaggttca tctccaggac atgatttaag
     2701 atactcaact gaaaaagttg agtcagtgtg gaactttatc aataaaatct ggaatggggc
     2761 acgtttcagt ttaatgaata tcggtgaaga ctttaaagtt gaagatatcg atttaagtgg
     2821 taacttatca ttagcagata aatggattct aacacgttta aatgaaacga ttgcaacagt
     2881 tactgattta agtgacaaat atgaattcgg cgaagttgga cgtgcattat ataatttcat
     2941 ttgggatgat ttctgtgatt ggtacattga aatgagtaaa attccaatga atagtaatga
     3001 tgaagaacaa aaacaagtta cacgttcagt attgagttat actttagaca atattatgag
     3061 aatgctacat ccattcatgc catttgtaac agagaaaata tggcaaagtt taccacatga
     3121 aggtgacaca attgttaaag cttcatggcc agaagtgcgt gaatcattga tttttgaaga
     3181 aagtaaacaa acaatgcaac aacttgttga aatcattaaa tctgtaagac aatcacgtgt
     3241 agaagtaaat acgccattgt ctaaagaaat acctatttta attcaagcta aagataaaga
     3301 aattgaaaca actttatcac aaaacaaaga ttatttaatc aaattctgta atcctagtac
     3361 cttaaatatt agcactgacg tggaaattcc tgagaaagca atgacatcag ttgtaattgc
     3421 aggtaaagtg gtattaccat tagaagggct aattgatatg gataaggaaa tcagccgttt
     3481 ggaaaaagaa ttagctaaac ttcaaagcga attagataga gtagataaaa agctctctaa
     3541 tgaaaacttt gtaagtaaag cacctgaaaa ggttataaat gaagaaaaac gtaaaaaaca
     3601 agattatcaa gaaaaatatg atggtgtgaa ggcaagaatt gaacaattaa aagcatagga
     3661 gttagtaaca atgaattacc tagagagctt gtattggata cacgaaagaa ctaaatttgg
     3721 catcaaacca ggtgttaaac gtatggaatg gatgctagca caatttaata atcctcaaaa
     3781 taacattaag ggtattcatg taggtggcac aaatggtaaa ggctctacag ttgcttacct
     3841 tagaacagct ttagttgaaa atggttatga agtaggtaca tttacgtcgc cgtttattga
     3901 aacatttaat gaacgaatta gtctaaatgg tgtgccaata tcaaatgacg ctattgtaga
     3961 attagtatca cgtattaaac cagtaagtga aatgatggaa cgtgaaacag atttaggtgt
     4021 tgcaactgaa ttcgaaataa tcacagcgat gatgttttta tattttggtg aaatacatcc
     4081 tgttgatttt gtcattgttg aggctggatt gggtataaag aacgattcga caaatgtctt
     4141 tacaccggtt ttatcaatct taactagtat cggtctagac catacagata ttttaggtgg
     4201 tacttatcta gatattgcta gggataaagg cgcgattata aagcctaacg ttccagtgat
     4261 atatgctgtt aaaaatgaag atgcattaaa atatgttcgt gaacgcgcaa ttgaacaaca
     4321 tgcaaagcca attgaattag atagagaaat tgttgttgta tcgcaaaatg atgaatttac
     4381 ttaccgttat aaagattatg aattagaaac aatcatttta agcatgttag gtgaacatca
     4441 gaaacaaaat gctgcattag ccataacagc tcttattgaa ttaaatgaac aaggattaat
     4501 tgaattagat ttcaataaga tgatagacgg tattgaatca gttcgttgga ctggacgtat
     4561 tgagcaggtg catgacaaac ctttaatcat tttggatggc gcacataatt cagagagtat
     4621 agatgctcta attgatacaa ttaaacagta ccatgataaa gaaaaagtag atattttgtt
     4681 ctcagcaata aacggaaaac cgattaacga gatggtcaaa catttaagtt taattgcgca
     4741 tacgttttat gcaactgaat ttgattttcc gaaagcgtta cgcaaagaag aaattgtagg
     4801 tagtattgaa aatgatgaaa tacaattagt agatgactac gttgaattta taaaaaatta
     4861 tcaaggtgat acattagtaa ttaccggtag tctgtatttc ataagtgaag ttaaatcaaa
     4921 attagacttt gaagcattat aaaactaaaa ctaaaatagc catgaagaat ctatttaatc
     4981 aataggtaga gcttcatggt tattttttat gctctaaatc gtatgtgttt ataaggttca
     5041 atttcttgcc attattaatt ttgccgagca gtaatgggtt gttaaagtat ccatcaatta
     5101 tgcttagcaa tattgaaaaa tttaaatgaa aaagcggttc aattcttgaa aaatagtgta
     5161 tatttacatt ttggtaataa ctaactataa tttacataag gaggttgtca tttggtagta
     5221 ttgttatctt atagttgcag ttgtattttt agttttctat atcaatttat atctatagaa
     5281 gaaacgtcat ttgattattt acatagaaga tcgaaatgtg attattgtaa ttcgtcactc
     5341 aaatggtatg aattaatgcc gattattagt tttttattat taaaagggcg atgtcgaaac
     5401 tgtcgaaagc gtatttccct aacacatttc ttaggggaaa cctttgcttt aatacctatc
     5461 gtctttatta agtatgattt cacatacgta aatgctacgc tatttataac tacatatgtt
     5521 tttctgctta tttttactat gaccgatatc acttctttaa tgttagattg tcgcttaatt
     5581 ataatttatt gtatcgtttc tctctcgtta agtatgattt atccagtagc ttttatcatt
     5641 attagtatga ccacgcatat attctacttt ttatttcggg catatattgg ttatggtgac
     5701 gttttactaa tatctgcact ttctttgttt ttccctctcc aattcactat ttatgtcatt
     5761 ttatttacat ttgtcattgc tggtttagtt gctttaatta ccatgatatt taagccgatt
     5821 aaactattac cccttgttcc atttatattt atttcctttt ttatcaattc acttttttat
     5881 aatgatatcc atcaattttt aggaggcgta tatttttgaa aattaaagaa atggtaactt
     5941 cagaaatgcc aagagaacgt ttgttaagcc atggtgcaaa aagtctttcg aatacagaat
     6001 tattagctat attaattaac accggaagaa aaggattctc gagcatagac attagtaatg
     6061 aactgcttaa atctgcttcg aatctgaatg aattgaaaaa atcttcaatt aacgatttga
     6121 taaaagttaa agggattgga ttacaaaaag caattacttt aaaagcagca tttgagttag
     6181 gggaaaggat ggggagaaga gctgaaaata atcgtataaa aataacgcaa ccaagtgatg
     6241 ttgctgatta tatgattcca acaatgaaag atttaacaca agaacatttt gtcattttat
     6301 tgttaaattc aaaaaatgta gtgattaaag aaacctgtgt ttttaaaggt acattaaata
     6361 gttcgattgt acatccacgt gaaattttta gtattgcggt gagagaaaat gccaatgcaa
     6421 tcatcgcagt tcataatcat ccatccggtg atgtaacgcc ctcacaagaa gatatcataa
     6481 caacaatgag gttgaaggag tgtggtttga ttttagggat agatttattg gatcatatta
     6541 taatcggtga taatagattt accagtcttg tagaagcggg ttactttgat gaaaatgatt
     6601 gaagttgata gaattaatga cgtatcttgt gtataatacc tacgaagtac tttcattgga
     6661 ggaaaaatag tgactctatt tattattatc ggggttctcg tgccaatggt ttataccatg
     6721 cagttaaata ttaaaaatga acctgtaaca aagcgcaatc ttttaataac attagcttta
     6781 tctacgttag gtattttagt aaccgcgtta gcaggtgtaa tcgttacgaa acaagctttt
     6841 cctttattaa gtgtagcaat tggctcaatt tttactggaa tcgtttgggg ccttttacta
     6901 agtggtagct acgcgctgat acgattttta tctaacgcat ttgggcgtaa gtaatagtaa
     6961 tctgttcatc aagtagtatc cgtgcttgaa aacaaactaa aactcctaat gtggaactag
     7021 ttaaaaaatt ctaaactagt acattaggag ttttgttatg cagaataaat ttaattgtta
     7081 aattgaaagt gcggtaaaaa tccactattt atttgaaaaa aatcgagcaa ccaaattaaa
     7141 ccttgatatc ctaagtaaat acataataaa acaagtccga cataaattaa aaatcgcaaa
     7201 atagaaagtc caactctaaa aaggaggatg actaatagtg ctattaaaat aattgttagt
     7261 atactcaatg ctacaaacct cctaatacgc ttttaaatca taacaaaatt ataagctatt
     7321 ttaaatgaac gtgcatcagt cctaagatat atcatgatta aggctgaaga ctgaggaaaa
     7381 tatacataat aaaatatata attagatgaa gacattggag gcgaactatg agattgatat
     7441 ttagtatgat taaaaacatt atagcagtaa tagcaattgt gataattgtt tatatcgcct
     7501 tgaaatacgc acctttttta aaagaacaag attggaatcc tgtaaatcac gataatgatc
     7561 aaatgaatca agttacgcaa cccactaatg atgcccaaca tgtttatgta agtggtgaga
     7621 aatattcatt aaaagaaaat gatttaataa agaatgtacc attaagccaa attaaaaatg
     7681 tatttaaaat gatagataaa caagagttta tggctgtgtc tggaatgaat cgcatggctt
     7741 ataatgatca atatattata ggtcaaagag gagacgaatt tattctttat aaatttggag
     7801 atgagtcaat gcgtgtttac aatactgaat ttgaaatgca acaagactta aatgaattag
     7861 ggcaaaattt acaactaaaa tctgaaaatg cttatcaata ggaaattatt gcataagaaa
     7921 tatattttag ttatcctgtc actagaataa atttaaaagg ttggtgtagt tcaaatggca
     7981 gaacaatcaa aacagaaaca agctaatgaa caacaaaagg cccagaactt attcgcacgt
     8041 tggagacaac ttcagaattc gaacagtgaa tcctcgaacg atacaaacaa ataattttaa
     8101 aatttaaatc gattatttaa actactagtg actggaatgt ttgctagtag tttttttgtt
     8161 tttaaaatgc cacaaatttt ttacggagtt gttcattttc aaagtaattc gtagttagtg
     8221 ctttatttca taatgggagt aatataaaat agaaaaggat atacaatatt ttgacataat
     8281 agaggtgttc tgggtgctta agttttttaa aaataacaaa ttaattgttg ttttatgtgc
     8341 aattatcgtt tttattgcat taattgggct gtccatacgt tcacaatctc aatcacctcc
     8401 tgaacaatat ataggtgatt ctgtgtcttt tggacaacga gttgtgagtt atccagttaa
     8461 ttttgttgct ggtacgattg gggacttttt taaaaaagga gattctaaag aatctaaaaa
     8521 taagattagc cagttagaat ctaagaacca acaattagaa gcggaaaatg aaaaattaaa
     8581 aaaagagctt gatttaaaag atatttcaaa atttgatcct atttctacta cggttttggc
     8641 aagaaatccg gatcagtgga tgaatacaat tgtaattgat aagggatcta aatcaggtat
     8701 aacttcaaat atggctgtga tgacatcaca aggttttgtt ggaagagtta ctaaagttaa
     8761 taaattttct tcacaagttg atttaatctc aactaataca cgtgcgggta aattatctgt
     8821 aaatatacaa cacggttcta aaaatatatt tggtttaatt gatcgttatg atgaaaagaa
     8881 ctcagaactt gtaattagtg acattaataa tagagataat atctcaaaag gtgataaagt
     8941 cgttacaagt ggattagctg atcaactacc aagtaattta tatataggag aagtgactaa
     9001 ggttcaaaat gatcaatacg gcttagctaa agaagttagg gttaagactg gtgcggactt
     9061 aacagatttg agtcatgttt atgttgcaaa aagagatcct aaaacaattc ctgatgatga
     9121 aagcagggat aaataatgcg tacactgtat tattttttga taggtatttt actattttat
     9181 atagatactg caattgggct tcttattcca atgcacatag gtaagtttga acttgtattt
     9241 gtaccacacc ttacatttat gtatatttta atgatggtag tgtatcgtgg ctttggcgta
     9301 tcattattgc tcagcatatt tttaggcgta atgactgatg tatactttgg tagtatttat
     9361 ggagtgtact tatttggcta tatattgttt ttagcactta tagatcgatt ctttaaaatt
     9421 ttttacaaag atcattcgat gttattcatt attatattag ccagcacctt attattagaa
     9481 gtctatgtgg cattaatata cggtatgtta ggattcattc aatttgatat tattcatttt
     9541 gtagtcttta gattattgcc aacattaatt atgaattttg tactgttgat tatgctttat
     9601 ccgttgatta taaagttcct taaaaaaaca aacaatgaca ttgacatgaa acgtcgtcaa
     9661 tggtaagatg caatagttga gtagttataa gtagctacat acaaccgctc aaatataggt
     9721 tcaagaacat ttttaatgaa gaaagcgatt ttcccacaga gtgcaaacgc ttgactcaat
     9781 aacgaaatgt cacctatgta tggcgtgact tattatacag gaggtgcaaa gtatgtttgc
     9841 tattattgaa acaggtggaa aacaaatcaa agtagaagaa ggtcaagaaa tcttcgttga
     9901 aaaattagac gtaaacgaag gagatacttt tacatttgat aaagtattat ttgtaggtgg
     9961 agattcagtt aaagttggag cgccaacagt tgaaggtgca acagttactg ctactgttaa
    10021 taaacaaggt cgcggtaaaa aaatcactgt attcacatac aaacgtcgta aaaattcaaa
    10081 acgtaaaaaa ggccatcgtc aaccatacac taaattaaca atcgataaaa tcaacgcgta
    10141 attattatga ttactgttga tattacagtt aatgatgaag gcaaagtaac agacgttatt
    10201 atggatggcc atgctgacca tggtgaatat ggtcatgata tcgtttgtgc tggagcttca
    10261 gctgtattgt ttggtagtgt taatgcgatt ataggattga catctgagag accagatatc
    10321 aattatgacg acaatggtgg tcattttcat ataagaagcg ttgatacaaa caacgatgaa
    10381 gcgcaactaa ttcttcaaac aatgcttgtg tctttacaaa ctattgaaga agaatataat
    10441 gagaatatta gattaaatta taagtgaggt gcattccgat gttaaaatta aacttacaat
    10501 tcttcgcatc taaaaaaggg gtaagttcta caaaaaacgg acgtgactct gaatcaaaac
    10561 gcttaggtgc taaacgtgct gacggtcaat tcgtaacagg tggttcaatt ttatatcgcc
    10621 aacgtggtac taaaatttac cctggtgaaa atgtaggtcg tggtggcgat gatacattat
    10681 tcgctaaaat cgacggcgtt gttaaattcg aacgtaaagg tcgcgacaaa aaacaagttt
    10741 ctgtatatgc agtagctgaa taattttgtc tagttaacac cagaagtgaa tcttctggtg
    10801 ttttttactt tttataaaat gtttttcatt attattttca ttatgttatt aaaaaatggg
    10861 aattttagac gttatactaa atgtgcaact gtataagagc ccctaatcac taaactataa
    10921 gggggacaaa ggaatacagt tgcagcgttt aaagaataaa ctgtaccaca attggtgctg
    10981 agaaatataa gtattttaaa gcaaagattt ataaaagtaa ctgcataaga gcccctaa
//
LOCUS       DAMQYT010000072        10881 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMQYT010000072 DAMQYT010000000
VERSION     DAMQYT010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10881
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.072"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            32..2731
                     /gene="clfB"
                     /locus_tag="PGA33_001425"
     CDS             32..2731
                     /gene="clfB"
                     /locus_tag="PGA33_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HDG6473244.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTTKPMST
                     QTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNSQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTASNFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDISKLSDSYYADP
                     NDSNLKEVTDQFKNRIYYEHPNVASIKFGDITKTYVVLVEGHYDNTGKNLKTQVIQEN
                     VDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPSP
                     DPDPDSDSDSDSGSDSDSGSDSDSESDSDSDSDSDSDSDSDSESDSDSESDSDSDSDS
                     DSDSDSDSESDSDSDSDSDSDSDSDSESDSDSESDSESDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSRVTPPNNEQKAPSNPKGEVNHSNKVSKQHK
                     TDALPETGDKSENTNATLFGAMMALLGSLLLFRKRKQDHKEKA"
     gene            2847..3608
                     /locus_tag="PGA33_001426"
     CDS             2847..3608
                     /locus_tag="PGA33_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDG6473245.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNSDTTVKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRINVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            3853..4308
                     /locus_tag="PGA33_001427"
     CDS             3853..4308
                     /locus_tag="PGA33_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDG6473246.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(4411..4521)
                     /locus_tag="PGA33_001428"
     CDS             complement(4411..4521)
                     /locus_tag="PGA33_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473247.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(4591..6015)
                     /locus_tag="PGA33_001429"
     CDS             complement(4591..6015)
                     /locus_tag="PGA33_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDG6473248.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSAEVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            6213..6413
                     /locus_tag="PGA33_001430"
     CDS             6213..6413
                     /locus_tag="PGA33_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473249.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQDKEQKDNKG"
     gene            6439..7104
                     /gene="nsaR"
                     /locus_tag="PGA33_001431"
     CDS             6439..7104
                     /gene="nsaR"
                     /locus_tag="PGA33_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HDG6473250.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQNQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            7115..8002
                     /gene="nsaS"
                     /locus_tag="PGA33_001432"
     CDS             7115..8002
                     /gene="nsaS"
                     /locus_tag="PGA33_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HDG6473251.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQSNQLHVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            8110..8865
                     /locus_tag="PGA33_001433"
     CDS             8110..8865
                     /locus_tag="PGA33_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6473252.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            8862..10862
                     /locus_tag="PGA33_001434"
     CDS             8862..10862
                     /locus_tag="PGA33_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6473253.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFVIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDSVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTITKLITNDYDLSFSDNSKSQ
                     IERQQTIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKNSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMIG
                     INNHHIFLTSYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
BASE COUNT         3834 a         1706 c         1943 g         3398 t
ORIGIN      
        1 ctaatctaga aattgaaatg gagtaatatt tttgaaaaaa agaattgatt atttgtcgaa
       61 taagcagaat aagtattcga ttagacgttt tacagtaggt accacatcag taatagtagg
      121 ggcaactata ctatttggga taggcaatca tcaagcacaa gcttcagaac aatcgaacga
      181 tacaacgcaa tcttcgaaaa ataatgcaag tgcagattcc gaaaaaaaca atatgataga
      241 aacacctcaa ttaaatacaa cggctaatga tacatctgat attagtgcaa acacaaacag
      301 tgcgaatgta gatagcacaa caaaaccaat gtctacacaa acgagcaata ccactacaac
      361 agagccagct tcaacaaatg aaacacctca accgacggca attaaaaatc aagcaactgc
      421 tgcaaaaatg caagatcaaa ctgttcctca agaagcaaat tctcaagtag ataataaaac
      481 aacgaatgat gctaatagca tagcaacaaa cagtgagctt aaaaattctc aaacattaga
      541 tttaccacaa tcatcaccac aaacgatttc caatgcgcaa ggaactagta aaccaagtgt
      601 tagaacgaga gctgtacgta gtttagctgt tgctgaaccg gtagtaaatg ctgctgatgc
      661 taaaggtaca aatgtaaatg ataaagttac ggcaagtaat ttcaagttag aaaagactac
      721 atttgaccct aatcaaagtg gtaacacatt tatggcggca aattttacag tgacagataa
      781 agtgaaatca ggggattatt ttacagcgaa gttaccagat agtttaactg gtaatggaga
      841 cgtggattat tctaattcaa ataatacgat gccaattgca gacattaaaa gtacgaatgg
      901 cgatgttgta gctaaagcaa catatgatat cttgactaag acgtatacat ttgtctttac
      961 agattatgta aataataaag aaaatattaa cggacaattt tcattacctt tatttacaga
     1021 ccgagcaaag gcacctaaat caggaacata tgatgcgaat attaatattg cggatgaaat
     1081 gtttaataat aaaattactt ataactatag ttcgccaatt gcaggaattg ataaaccaaa
     1141 tggcgcgaac atttcttctc aaattattgg tgtagataca gcttcaggtc aaaacacata
     1201 caagcaaaca gtatttgtta accctaagca acgagtttta ggtaatacgt gggtgtatat
     1261 taaaggctac caagataaaa tcgaagaaag tagcggtaaa gtaagtgcta cagatacaaa
     1321 actgagaatt tttgaagtga atgatatatc taaattatca gatagctact atgcagatcc
     1381 aaatgactct aaccttaaag aagtaacaga ccaatttaaa aatagaatct attatgagca
     1441 tccaaatgta gctagtatta aatttggtga tattactaaa acatatgtag tattagtaga
     1501 agggcattac gacaatacag gtaagaactt aaaaactcag gttattcaag aaaatgttga
     1561 tcctgtaaca aatagagact acagtatttt cggttggaat aatgagaatg ttgtacgtta
     1621 tggtggtgga agtgctgatg gtgattcagc agtaaatccg aaagacccaa ctccagggcc
     1681 gccggttgac ccagaaccaa gtccagaccc agaaccggaa ccaagcccag acccggatcc
     1741 ggattcggat tcagacagtg actcaggctc agacagcgac tcaggttcag atagcgactc
     1801 agaatcagat agcgattcgg attcagacag tgattcagat tcagacagcg actcagaatc
     1861 agatagcgat tcagaatcag atagcgactc agattcagat agcgattcag attcagatag
     1921 cgattcagaa tcagatagcg attcggattc agacagtgat tcagattcag acagcgactc
     1981 agaatcagat agcgactcag aatcagatag tgagtcagat tcagacagtg actcggactc
     2041 agacagtgat tcagactcag atagcgattc agactcagat agcgattcag actcagacag
     2101 cgattcagat tcagacagcg actcagattc agacagcgac tcagactcag acagcgactc
     2161 agattcagat agcgattcag actcagacag cgactcagac tcagacagcg actcagactc
     2221 agatagcgac tcagattcag atagcgattc agactcagac agcgactcag attcagatag
     2281 cgattcggac tcagacagcg attcagattc agacagcgac tcagactcgg atagcgattc
     2341 agattcagat agcgattcgg attcagacag tgattcagat tcagacagcg actcagactc
     2401 ggatagcgac tcagactcag acagcgattc agactcagat agcgactcag actcggatag
     2461 cgactcggat tcagatagcg actcagactc agatagtgac tccgattcaa gagttacacc
     2521 accaaataat gaacagaaag caccatcaaa tcctaaaggt gaagtaaacc attctaataa
     2581 ggtatcaaaa caacacaaaa ctgatgcttt accagaaaca ggagataaga gcgaaaacac
     2641 aaatgcaact ttatttggtg caatgatggc attattagga tcattactat tgtttagaaa
     2701 acgcaagcaa gatcataaag aaaaagcgta aatacttttt taggccgaat acatttgtat
     2761 tcggtttttt tgttgaaaat gattttaaag tgaattgatt aagcgtaaaa tgttgataaa
     2821 gtagaattag aaaggggtca tgacgtatgg cttatatttc attaaactat cattcaccaa
     2881 caattggtat gcatcaaaat ttgacagtca ttttaccgga agatcaaagc ttctttaata
     2941 gcgatacaac tgttaaacca ttaaaaactt taatgttgtt acatggatta tcaagtgatg
     3001 aaacgacata tatgagatat acaagcatag aaaggtatgc gaatgaacac aaattagctg
     3061 tgattatgcc caatgtggat catagcgcat atgctaacat ggcatatggt catagctatt
     3121 atgattatat tttggaagtg tatgattatg ttcatcaaat atttccactt tccaaaaagc
     3181 gtgatgacaa ttttatagca ggtcactcta tgggaggata tggcacaatt aaatttgcat
     3241 taacacaagg ggataaattt gccaaagctg taccattatc tgctgtgttt gaagcgcaaa
     3301 atttaatgga tctagagtgg aatgattttt caaaagaggc cataattggc aatctttcaa
     3361 gtgttaaagg aactgaacat gatccgtatt acttgctaga caaagctgta gctgaagata
     3421 aacaaattcc aaaattgctc attatgtgtg gtaaacaaga ctttttatat caagacaact
     3481 tagattttat cgattattta tcacgcataa atgttcctta tcaatttgaa gatggaccag
     3541 gagatcatga ttatgcatat tgggatcaag cgattaagcg tgctataaca tggatggtga
     3601 atgattaatt atttcttgga aaatatgtgg ctgcattaaa tacacagagt gagagataca
     3661 aactatttac gcacgactaa catttctaag tgtttaaatt atttttgtat taatatgatt
     3721 ggcgcaattt gctgatacac aaaaatgttt ctcgtgaaac ttagatttag cttatagttt
     3781 tatcatcatt tgtatgactt acattataaa ttttattata atgaggttaa cgctttgaaa
     3841 ggagtcatca tcatgtcgac caataaaaac gattatgagc atatgttgtt ttattttgca
     3901 tataaaacct ttattactac cgctgatgaa attatagaga agtatggtat gagtcgtcag
     3961 catcatcgtt ttttgttttt tatcaataaa ttacctggta ttactattaa atcattacta
     4021 gaaatattag aaatttctaa acaaggatca catgcaacac ttcaaaaatt aaaagagcaa
     4081 ggtctcatta ttgaaaaagt tttagagact gatcgacgtg tcaaaaaatt atattcgacg
     4141 gataaaggcg atcaactcat tgctgaattg aacaaggcgc aagatgaatt attgcaaaat
     4201 atatatcaac aagtcggttc ggattggtat gatgtgatgg aagcattggc taaagggcga
     4261 cctggctttg attttattaa gcatttgaaa gatgaaaaag aaagctagca tcagaaatgt
     4321 taaaaatctt cgcattctta aatttaaaaa atatgtcaaa aagtgtataa taaaaacata
     4381 taatttaatt gaactcagtt tcaacacatc ttagaaagga gtttgaatga tgaaaaaatt
     4441 agcagttatt ttaacattag ttggcggttt atacttcgca tttaaaaaat accaagaacg
     4501 tgttaaccaa gcacctaaca ttgagtacta aattaaacca taaaaaattc ccgaacacct
     4561 tgttatagtg ctcgggaatt tttttatgct ttacttgaat atatcaaata ttatttttgc
     4621 gctttctgta ttttcgatat taccactaaa tgattctgat ctaggtccgt aagcgtaggt
     4681 attaacatcc tcgcctgtat gtccatcgga agtccaccct gtataagatt tatcatttac
     4741 tggcttctga atagcgtgtt gtagggcttt tgtttgcgtt tctacttctg cggatttttc
     4801 gtctttttct tttttaagta gtctttttag ctttttattc tcttttttaa cctttttcat
     4861 atcatcttgt gaaaattcaa atccataacc ttcattaata actttttcag ggtcttcacc
     4921 tttagccatt ttttctgtca tatatgatcc agagtgtttc atagatttaa tcggttgagg
     4981 attccattcg tatcctttat ctttaccaat tgttagacca ccagtagagt gatctgctgt
     5041 tgcaacgaca agtgtatctt tatgtttctt agcatattga atggcgtcat cgaatgcttt
     5101 ttcaaaacct tccatttcag acataacgcc tgtaatatcg ttggaatgcg ctgatttatc
     5161 tatagaagca ccttcgacca ttaaaaagaa tcctttttta ttgcgctcaa gcttactaag
     5221 tgcactttgt tgcatatcag ctaatgatgg ttcgtcttta gaagcatcta ttgcaagtgg
     5281 catattttta tctgcaaaca aaccaagaac tttatcttta tcagattttg ataactcctt
     5341 actgttcgtg gcaaggtcgt aaccatcttt tttgaatttt ttatctaaat tgccattact
     5401 tttaccgaaa tatttagcgc cgccgcctaa taaaacatca actttatgct ttccgttgat
     5461 tttatcttta taaaattgtt tagcgatttc gtttttatca tctctagaag tcacgtgtgc
     5521 agcatatgct gctggtgttg catctgttaa ttcagctgtt gaaacaagac cagtcgactt
     5581 acctttttct tttgcacgtt caagcaccgt ctttactttc tgcttgttac tgtcaacacc
     5641 gatggcacca ttatatgtct tatgaccaga actaaaggct gttccgccag ctgcagaatc
     5701 agtaatattc tgttttgggt catttgaata tgtacgattt gtgcctttta aatatgaatc
     5761 aaaagcagta ggggtcattt ctttagcatg cggatcattt ttataataac gataagctgt
     5821 gttaaatgat ggacccatgc catcgccaac taaaaagata acattttttg gatttttagt
     5881 attaccaacc gcgaaacttt catctttaga acttttatcg gattgcgcaa ttgcaggtgt
     5941 gacagaacta aaaaccgttg acacgataat aaggttagca actgcaaatt ttgtggcttt
     6001 tttaactgat aacataagac atcctcctga gtatatgact atgtcttcag tgtaaaagag
     6061 gaattttgag caattatgta gttttagtta aaaatatgta aacagagtga tttagaataa
     6121 caaaaaatga atatatatga caatttgtta tagaaagcgt tagaatagaa gcgtgtgaaa
     6181 atatagaatt aaatataatt tgaggtggaa aaatgatact agtaatgtta tctccattat
     6241 taatcatatt ctttatagtg ttgtctattt tagaagagcg taaacgtacg aagaaaaagc
     6301 aactcgagaa agaaaaagca aatacactaa atcaaaatac aaatgacacg gaaagttcaa
     6361 atcaagagcc gtcattgcag caggataaag aacaaaaaga taacaaagga taattcaatt
     6421 gaaggaagaa gattatagat gaaaatatta attgttgaag atgattttgt tatagcagag
     6481 agtttagcat ctgaacttaa aaaatggaat tacggtgtta ttgtcgttga acaatttgat
     6541 gatatactgt ctatctttaa ccaaaatcaa cctcagcttg tattgctaga tattaatttg
     6601 ccaacgttaa atggttttca ttggtgtcaa gaaatccgaa aaacatctaa tgtgccaatt
     6661 atatttatta gttcccgtat tgataatatg gaccaaatta tggcaataca aatgggggga
     6721 gatgatttta tcgaaaagcc atttaacttg tcattaacga ttgccaaaat tcaagcatta
     6781 ttgagacgaa cttatgactt gtcagtagct aatgattcat tgacggttaa aggttgtaca
     6841 ttaatattag atgaagcaaa agtcgtgtat caagaacaaa acatacagct atctttgact
     6901 gaattacaaa tattaaagtt attatttcaa aatgaagata aatatgtaag tagaactgct
     6961 ttaattgaaa aatgttggga atcagaaaac ttcatagatg ataacacatt agctgttaac
     7021 atgacgcgcc tgcgtaaaaa attaaatact attggcgtta atgattttat cattacaaag
     7081 aaaaatgtcg gatataaagt atagggtgaa tgcaatgacc tttcttaaaa gtattactca
     7141 ggaaatagca atagtcatag ttatttttgc tttgtttggc ttaatgtttt acctgtatca
     7201 tttgccatta gaagcatatt tactagcact tggcgttatt ttattattat tactcatatt
     7261 cataggtatt aaatatttaa gttttgtaaa aactataagc caacaacaac aaattgaaaa
     7321 cttagaaaat gcgttgtatc agcttaaaaa tgaacaaatt gaatataaaa atgatgtaga
     7381 gagctacttt ttaacatggg tacatcaaat gaaaacaccc attactgcag cacaactgtt
     7441 acttgaaaga gatgagccta atgttgttaa tcgtgttcgt caagaggtta ttcaaattga
     7501 taactataca agtttagcac ttagttattt aaagttatta aatgaaactt ctgatatttc
     7561 tgtcactaaa atttcgatta ataatatcat tcgcccaatt attatgaaat attcaataca
     7621 gtttattgat caaaaaacaa aaatccatta tgaaccttgt catcacgaag tattaactga
     7681 cgttagatgg acctctttaa tgatagaaca attaataaat aatgcactta agtatgcgag
     7741 aggtaaagat atatggattg aatttgatga gcaatccaat caattacacg taaaagataa
     7801 tggtatcggt attagtgaag cggacttgcc taaaatattt gataagggct attcaggtta
     7861 taatggccag cgccaaagta actcaagtgg gattggttta tttatcgtaa aacaaatttc
     7921 aacacacaca aaccatcctg tttcagtcgt atctaaacaa aatgagggta caacatttac
     7981 gattcaattt ccagatgaat aaaaactttc aatattgtaa gtatactagt aacatttttt
     8041 tactaattta aatgttatta gtattttttt gttttaatat agaactaaca aagaaatgag
     8101 gtgcatgcca tgttgctaga agtgaaacat gtaaaaaagg tttatggtaa aggtttgaat
     8161 gctacgacag cacttaatca aatgaattta tcagttggag ctggtgaatt tgttgcaatt
     8221 atgggtgagt ctgggtcagg gaagtctaca ctactaaatt taattgcttc ttttgatgga
     8281 ctaactgaag gtgacattat tgtggatggc gcacatttaa ataatatgaa aaataaaagt
     8341 aaagcattgt atcgtcaaca aatggtaggt tttgtttttc aagattttaa tcttttacca
     8401 acaatgacga ataaagaaaa tataatgatg ccattaattt tagctggtgc taaacgaaaa
     8461 gatatagaac aaagggtaca tcagttggca gtacaattac atttagaggg attcttaaac
     8521 aagtatcctt ctgaaatctc tgggggtcag aagcaacgca ttgccattgc acgtgcatta
     8581 gttactaagc cgacgatttt actagccgat gaacctacag gtgcacttga ttcaaaaaca
     8641 tcaaaggcat tgatgatgtt atttcaagag attcatcaat tggaacagac aattttaatg
     8701 gtaactcatt caaatatcga tgcgtcttat gcagagcgag tcatttttat taaagatggg
     8761 cgtctatatc atgaaatata tcgtggtgaa gaaagtcaat tagcttttca acaacgaata
     8821 acagatagct tagcacttgt gaatggagga agtgtcaata tatgaagtta agattgttat
     8881 ggcacatagt gcgacgtcaa tttattacgc agcgacttgt aatcattcca ttcattttag
     8941 cggtaagtgt actattcatg attgaatata cgcttgtgtc aattgggtta aatagctaca
     9001 taaaacagaa gaatgacttc ctagtaccat ttattatcat agctaatttt tttatggcgc
     9061 ttttaacttt tatttttatt ttctatgcaa atcactttat gatgtcacaa agacgaaaag
     9121 agtttagcat ttttatgaca ttgggcatga ccaagaaaag tatgcgttta attgtagtga
     9181 tggaaactat cttacaattt gtgataattt cagtcgttag tattgccggc ggatacttac
     9241 ttggtgcgat atttttcttg tttatacaga aaataatggg cagtgaagtt gcgacgttaa
     9301 ggtattatcc atttgactct gtagcgatgt ttattacttt gattatcatt gctgtattaa
     9361 tgggcatgct acttatattc aacttgttta gtattaattt tcaacggccg ataacttatc
     9421 aacatcgttc cgattctagt gtcatatcac gatggttgcg ttacgtttta attgttatag
     9481 gaagcgcagc actatattta ggttacttta ttgcattaca acaagatacg acgtttggtg
     9541 ccttttttaa aatatggatt gtcataggat tagttattat cggtacttat gcattttttg
     9601 taggtataag tgaaataatt attagtatat tgcagcaggt atcaaaagtt tactatcatc
     9661 cacggtattt ttttgtggta gttgggatgc gtgtacgtct taaaatgaat gcagtcagtc
     9721 ttgcaacaat cactttgctg tgtacatttt tgattgtaac gctcacaatg acattaacaa
     9781 cctatcgtga tatgaatcat accattacga aattgattac gaatgattat gatttgtcat
     9841 ttagcgacaa ttctaagtca caaatagaac gtcaacaaac aattgagaat attaaacgtg
     9901 atatacaggg ttcggttaat gcaaaagact tcaaagtata tgaaacgaca ttatttagag
     9961 cttctttaga aaagaatcgt gcttattaca agttaaaaca ggctagtgat gatattccaa
    10021 tagacttcat tggtaatgaa ggcgcgatat tttcaagaca atcagtaatg attacagctt
    10081 ttactgcaca agattataat cgatatcacc aaaacaaatt acacttagac aatcaaagta
    10141 taggtatgat tacgaatgta cctattttta aaaagcagtc taaggtcggt cttaataatg
    10201 aagtctttaa agtgaaacaa cttgacgcac atgcgttaaa tttgatgatg attcaagata
    10261 gtatggtttt aattgttaaa gataacaatc aattacaaaa agtaaaagga ttttatgcac
    10321 atgaacaaaa gaattcgaca atatcgataa actttaatgt atttggaaag acgaaattaa
    10381 caacatcaca aatacaaaag ctatcgaaaa aatacgatgc gtcaataaat tctaaatcag
    10441 aaatgctaat ggtttgggat cgtttgacag gtggactaat atttgtaggt ggcgtagtat
    10501 caattgtact tgtcattgga atatttctaa tgatgtatta taaacaagtc tcagaagcct
    10561 atgcaaatca gcacaattat gacattatga aaaaacttgg tttagataat ggaagaatag
    10621 caaagataac acgaaatcaa atgactttct tgttcgcaat accaataaca gtagcactga
    10681 ttcacacatt aatttctagt aacattgtat atacgttact aaatatgata ggtattaata
    10741 accatcatat atttttaaca agttacgtac tagctgtcat tataatttcg tttctatata
    10801 tggcgatgta caaaataact tcctatattt atgctaaagt tattcatcaa caacgcaatt
    10861 aaccatgttt cagtgaaatc a
//
LOCUS       DAMQYT010000073        10813 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMQYT010000073 DAMQYT010000000
VERSION     DAMQYT010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10813
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.073"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(278..970)
                     /locus_tag="PGA33_001435"
     CDS             complement(278..970)
                     /locus_tag="PGA33_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDG6473254.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFNFADVTKEKCIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKI"
     gene            1206..1664
                     /locus_tag="PGA33_001436"
                     /pseudo
     CDS             1206..1664
                     /locus_tag="PGA33_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1651..1953
                     /locus_tag="PGA33_001437"
     CDS             1651..1953
                     /locus_tag="PGA33_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473255.1"
                     /translation="MRTNDHITDLDAIELSSGWNYNSKLETGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINSMSQNEGKSVDALKNSTASLKNNLTKLKEQLDKTTH"
     gene            2107..2466
                     /locus_tag="PGA33_001438"
     CDS             2107..2466
                     /locus_tag="PGA33_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473256.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALNL
                     LGLATLSLLDTFSCNKK"
     gene            complement(2551..3161)
                     /locus_tag="PGA33_001439"
                     /pseudo
     CDS             complement(2551..3161)
                     /locus_tag="PGA33_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            complement(3240..3716)
                     /locus_tag="PGA33_001440"
     CDS             complement(3240..3716)
                     /locus_tag="PGA33_001440"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HDG6473257.1"
                     /translation="MAVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGQKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(3719..4219)
                     /locus_tag="PGA33_001441"
     CDS             complement(3719..4219)
                     /locus_tag="PGA33_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6473258.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(4454..5221)
                     /locus_tag="PGA33_001442"
     CDS             complement(4454..5221)
                     /locus_tag="PGA33_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDG6473259.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILKGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(5218..5910)
                     /locus_tag="PGA33_001443"
     CDS             complement(5218..5910)
                     /locus_tag="PGA33_001443"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HDG6473260.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNRDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(5927..6589)
                     /locus_tag="PGA33_001444"
     CDS             complement(5927..6589)
                     /locus_tag="PGA33_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HDG6473261.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
     gene            complement(7437..7997)
                     /gene="icaR"
                     /locus_tag="PGA33_001445"
     CDS             complement(7437..7997)
                     /gene="icaR"
                     /locus_tag="PGA33_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HDG6473262.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
     gene            8161..9399
                     /gene="pgaC"
                     /locus_tag="PGA33_001446"
     CDS             8161..9399
                     /gene="pgaC"
                     /locus_tag="PGA33_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDG6473263.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            9363..9668
                     /gene="icaD"
                     /locus_tag="PGA33_001447"
     CDS             9363..9668
                     /gene="icaD"
                     /locus_tag="PGA33_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HDG6473264.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            9665..10537
                     /gene="icaB"
                     /locus_tag="PGA33_001448"
     CDS             9665..10537
                     /gene="icaB"
                     /locus_tag="PGA33_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HDG6473265.1"
                     /translation="MKYRKFIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            10524..>10813
                     /locus_tag="PGA33_001449"
     CDS             10524..>10813
                     /locus_tag="PGA33_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDG6473266.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYS"
BASE COUNT         3678 a         1828 c         1537 g         3770 t
ORIGIN      
        1 atatatctat accatccacc ttttatttat aaatagttaa tttacaacta aacgataaat
       61 attatatgca aaatacatct ttaatattaa agtaatacca atattttttc aataaaccta
      121 gtgtaatata tgtgtaattc taaaagattc ttctttaaaa atataaatac cacgacatat
      181 tgctttaaca ttttcattta taaagcgaaa aaatgcatcg ctactaagtt gaatgtttag
      241 taaggatgca tttaattcac taaaatgatt aaattactta tatcttttca tctgattgat
      301 tatcgaaatt tcttccttct aaacctgcta actcttcttt agaagctgca ggtgctttca
      361 tttcaaatat ctcattcact actgtgtaat cgtaatatcc taatctggca ataggtttaa
      421 tcgacttaat gtccaattta ccattatcaa gaataacctt atcgtcaata tgaacttggg
      481 caactcttcc tataacaata tctacggtag atactggatc tccagttgga atacgaatcg
      541 tttgaacgta ctcacattca aaatgaactg gcgattcttt tacacgatat cctggagctt
      601 ctatacattt ttcctttgtt acatctgcaa aattaaattc atcctcttct ggtggcaatg
      661 ctttcgatga taaattaact gcttctctta aatcatacgt tgccatattc cacacaaacc
      721 aacctgtctc ttcagcattt ttcactgtat ctttacgttc gtgatcacca agaacggatt
      781 gatttgctgc gaacataacc ataggcggat cccaagttaa gttttgatac tgactataag
      841 gcgctaaatt atctttccca tctttcgata cagtagagat ccaccctatt ggacgtggta
      901 ctgtactact tttaaatggg tcgtgcggta aaccatgact tcttacacct tgttttggcg
      961 aataattcat actatcttca ccccttataa gtaattacat ttaaggttac gccctcttta
     1021 cataagcgtc taatataaat aaacaattta tttataagta gaaactatat atgacgtggt
     1081 tgcttataat ttgcgttctt gattcgaaaa attcagataa ggatttatac aattaatatt
     1141 tatgatatct tttgtaaatt taattaatta tagttacttc aatcatgatt agtttataat
     1201 aataaagtga aattgaaaaa gacagctatt atgcgatgag cgaaaaactt caagtaaaac
     1261 aagaatttaa cggcgttaca aggcaatact tgaatttcag aacagatagt atcgatgatc
     1321 tggataagtt caaaaaagaa ttcaaacctg ttatgcactt aaaaggtgat gcattcaatc
     1381 aacagttaca atctttgatt aacaaatatc cacaaataca aaaaaatatg aaatcagagt
     1441 tcattgctta ttatgataaa gaaaaaaata gagaaacagt aaaaaactat gcttggaacc
     1501 ttcaaaaatc tataaatgac attatgcaat catatcctag cacaaaattt gtacagtttt
     1561 ataaagatga tgtttccccg tcaatggtag atggaaatgg ccgtttaaaa tcggatacta
     1621 atgtaatttc catcgaggga ggaaagtatg atgagaacaa atgatcatat aaccgattta
     1681 gatgctattg agttaagtag cggttggaat tacaattcga aattggagac tggcaaaaat
     1741 gcactactat cagatagcct tttaagagat attaaagtat taagttctaa tgctaaaggc
     1801 gacgctgaca tactagaaac agaacttcgt gctgtaataa actcaatgtc tcaaaacgaa
     1861 ggtaaatctg ttgatgcact taaaaatagt accgcttctc taaaaaacaa tctaaccaaa
     1921 ttaaaagagc aactcgacaa aactacacat taatttcata cattttgagt gaacagaatt
     1981 atatttccat agcaaacatt cctaaactac acatacgtta cataattgat tcatttttat
     2041 agaaacgggt aaaaatgata aagtaatcat ctttcacaaa aaatcaaata ttgggaggaa
     2101 ttaaatatga ttttgcgtta cgcaacgaat ttaaaacttg cgaaagagct ataccaagcc
     2161 agtaaaccta aattgcaaga agataagggt atgattgaac tgtttgaaca tacttttggc
     2221 ttgcaaaaag aacttgtaaa atacgtcggc attgcagaag caactactgc tgctttatac
     2281 tcagctagct ttatcaataa aaacatttct cgccttgcat cactatcaac gataggcatt
     2341 ttaagtgtag ctgcttacaa acatttcgaa gctggccaag gtaaaaaagg tgcacaacat
     2401 gcattaaatt tattaggtct tgctacttta agtttactag atacattttc ttgtaataaa
     2461 aaatagtctt cattttagaa gcacatactt tagcacctgt gtacttttga ttcaattcat
     2521 aaacggtata ccgctatagc atgtgtttta ttatttttct ggtctcttta tcgtaaatat
     2581 tccccctaat ttagcataat catttccggc taaacgagaa acagcctgta atgcgtgtgg
     2641 attgatttta aaatcaccct cataaatact gtcatcaata tgataagtta caatttcacc
     2701 tattattaaa tcagccccat ctaatacatc tccaagcaat atcatttgcg atagtttaca
     2761 ttcgaatctc attttcgcat ctttaattcc tggcgtctta atcgttgtag atgttaaaag
     2821 tgataattct gtacgactca actcactgtc accatatgct aacggcgctg cagtctcatt
     2881 aatatcttga acattatctt cgtctgtaat atgcacaaca aagtcccagt ccgttctata
     2941 tttaatgcag tatcttttct cttacctcct gcacgttgaa ctgcaatagc aatcattggc
     3001 ggatgattat taacaatatt aaaaaagcta aatggtgctg catttactga tgcatcttga
     3061 tttaatgttg taacaaaagc tataggtcgt ggaataattg aaccaattaa taatttatag
     3121 ttttctctag cagttaatga ttgtgcatca aacgtataca taatacctac ctcttttcta
     3181 agtatatcta ggtatttctc cgattttggt taatttaaac atctattctc ctctgaaaat
     3241 cacttgtatt tatttagcaa atcttttgaa atatgacaca tatgcatatc ttctggatat
     3301 ttttctaaat gttgctgatg ttcttcagca cttttaatgt agttagacag cggtaagact
     3361 tccactgcaa tttgatctct gtctttacgt cgttcaatga actgacgcgc ttcaattaag
     3421 tggtcatcta cacaactata taaacccgtt cgatactttt gtccaatatc atttccttgt
     3481 tgattcacac tgtaaggatc aatgatttca aataaataat tcataatgtc tgtaattgtt
     3541 aacatacgat catcgaaatg aagtttgaca cattcagcat aaccatcata cggaccgtct
     3601 aatttagagc ttcttccatt tgctcttcct gcttctgtat gtataattcc aggtattgtt
     3661 gcaaaaaatg cttcaacacc ccataaacat cctcctgcta cataaacaac tgccatattt
     3721 acacctcatc atcctttttt atatttttaa caaggttata ccatttaata ccgccatgac
     3781 atgattctga tacaccttca ttacgatacc catatttttc ataaaatgaa attaatgatt
     3841 ctcgacatgt taacgttaca ccatgtcgat gatgattctt agcaagagtt tcaaaatagt
     3901 ttagtaagcg acctgcaata ccctgacctt gataatttgg tgctacaaca agacctaaca
     3961 cactaatata gccaccttca ctattatttg tggagacatt tttaaataaa tcatcgctaa
     4021 tgtaacgctc ttttatgact ggaccgttga tgtatccaat tacttcatca ttcttttcag
     4081 ctacaataaa agtttcttga atttgttcaa ttctcaattt caatgcctct cgtgttgcag
     4141 cttcttccgg tgaaaaacca atattttcaa gtgcaataat ttgatctaag tcctgcatga
     4201 ttacttttct taataccata aaaacacttc cttagcccta tatatcaaaa gtaatacctt
     4261 catctttata ttcattattt tcaaactacc ttcactataa aaaacgagtc tttttattga
     4321 aagccattcg ccttaatcaa ttagacaagt tgtattatac gaattagtaa ataatcatga
     4381 ttataattca ttttaagcat actcaaaaag actggtacat gtataccagt ctaattcgaa
     4441 aaaatatatt tagttaaaac catttcaaaa acgacttcac ttttcctggc ttagtcggtc
     4501 tgtctaaata aacaaaatta tcattgatta ctgaactggc atttcgaagt aatgcttcaa
     4561 tatcattcga atatttcttc aatttatgat tgtgaaataa ttcttgcatc aaaaatggtc
     4621 tttggtcaca tgaatgtgca tctgaagcta caaaatgagc caaattacat tctataaatt
     4681 gtaatgataa cttttgaatg tttttaccaa atccaccaac taaagaactc gatgttaatt
     4741 gactcagtgc cccatttgca accaattcat ataatatttc cggatttttg gcgatacttc
     4801 tatttctttc aggatgtgca atgattggta tgtaacctct cgattgtatt tcaaaaaaca
     4861 attgttttgt ataatgtggt acttcgcccg ttggaaattc aattaataaa tatttcgaac
     4921 gattaatacc ttgaatacta ccattatcta agcctttcag aatcgaatct gtaattctaa
     4981 tttcttgccc gggaaataat ttaatatcca atgcttgaac ttctggatgc gttcttaact
     5041 ccgccaattt cacaagcact tgttgaaatg tattatcata tctcggatgc aaatgatgag
     5101 gtgtcgctac aatacttgtt acaccttcat ccttagcttg ctttaatagt gcaatactct
     5161 tttcaattgt tttaggacca tcatctatat caactaatat atggttatga atatcaatca
     5221 tgattcatca gtcccataat atgcatagta actagcactt ttatctttag gcattctatt
     5281 taagactaca cctaataatt tagcacctgt tgcttcaata agttcttttc cttttttaac
     5341 ttcatctcta ttattatttt ccgaattaac tacgtagaca acattgccgg taaactttga
     5401 aaataattgc gcatctgtaa ctgtgttcac tggtggcgta tcgataatta caaagttata
     5461 attcatcaat aatgtgtcat acaaatttgc aaatgccctt gatgtaatta actctgacgg
     5521 attcggtggg attggcccag acgtcaagac gtctaaatct tgaatttcag ttgagataat
     5581 actgtcttga taagttgacc aatttagcaa taaacttgat aggccttcat tgtttggcaa
     5641 attaaaaata taatgctgcg taggtttacg catatccccg tctacgatta gtgttttata
     5701 acctgcttgc gcatatgcaa ctgctaaatt tgctgcaatt gtagacttac ctgcgcctgg
     5761 tgcctctgaa gtgattacaa tgctttgaac tgcactgtca ggatttgcaa acataatatt
     5821 tgatcttatg cctcgaaatt tctcgctaat aggtgacttt ggttgttcat ggacaattaa
     5881 acttgatgta cttcttcgtg tattcgtcat ggtaattcct cgtaaattaa aatttttgta
     5941 ttgaacctaa aataggtaat cctagttgcg attcaacatc ttcttctgtc ttaatacgct
     6001 tatctaataa ttcttttaag aaaataatca atattgctaa aacaatacca acaataatgc
     6061 tgataactaa gttgacagat actattggag atacttttac agcattatca tgtgctgagg
     6121 aaagtatcgt aacattatca acactcataa ttttaggcat gtcatgagca aaaactttag
     6181 atattttatt aacaattttg tcagattcag atttattccc agtggtaact gatacagtaa
     6241 taatttgaga gtttgtttga ttggttactt ttaaaaatga attcaactca gctgttgaat
     6301 actgaccatc aaattctcta gatactttat ctagaattct aggacttttg ataatttccg
     6361 tatatgtatt aacagactgc aaactacttt gaacattttg gaaagctaaa tcacttgagg
     6421 actttttcat gttcactaat atttgagtag aagcagtata tttgtcaggc ataacaaaaa
     6481 aggttaatgc cgcacttact acaagacata ttgccggtaa aataagcaat aatttaatat
     6541 tcttctttag aatatttaat agttttacta aatcaaactt ttctttcatg gtttcctcca
     6601 cataatcaat cattgtattc attatgtatg ttttataaat cggacaatta tatctagttt
     6661 aacgaccaca aaacatacac aactacattt tctctaatta tttatataaa tattttatcg
     6721 tttaaaatta tatcatgatt ctctaccatt atgtataact tatttatatt tttgcacaag
     6781 atataatatt gtccaacttt aaatatccaa acctattaat aataaaacta gataccatcg
     6841 tactctatca tggctttctt ataatcgagt agaagcatca tcattacttg attatttgct
     6901 ctttacaaca ccgagcgtgc ccgtactcgg taattcaata ccttgcgtaa cccgtcactg
     6961 tgagttgggt taatgataat aaagcccaca ccttttaaaa agatgtgggt aatttatata
     7021 atttttattt acatttttaa cttataaaaa aagcgcctat gtcatgattt accatcacat
     7081 aggcgcttat caataaatta ttacttatta ctttccattt catctaattt atgcggattt
     7141 cctgtaatta gatgacaact tattcttttc aggggaacat tacactttta taatatgttc
     7201 aaagacaaac ttaaccattc acaaatataa agaataatat tatcaaatca ttgaacaaat
     7261 cgtattttgc aacaattgat atttatatta atgtattgca tttaatttat aaaattcata
     7321 tacatcttaa tattctcaat atcgatttgt attgtcaact ttatatagat ttaaaaaaat
     7381 aatctcatgt ctttttttac aaaagtaagt taattattac aaactagtaa caaaaattat
     7441 ttcttcaaaa atatatttag tagcgaatac acttcatctt tgaattgact tttactttct
     7501 tccactgctc caaatttttg cgaaaaggat gctttcaaat accaactttc aagaaacagc
     7561 aatattaaat tctgaaagtc ttcttttgtc atctttatct ttgattcatc atagaatttt
     7621 gctatctctt tacttaatga ttgatttaaa tcttgtattt gtccgtaaat atttccagaa
     7681 aattcctcag gcgtattaga taattgaacg tacattctaa tatacctttc ttcgatgtcg
     7741 aaaataaact caaataagaa ttgatataaa gcatcaattg aatagttcga tttattttga
     7801 ttcatcataa taatattatt aaggtaatca aaacaacatt taacactttg ttcgtaaata
     7861 ctttttttcg agtcaaaatg gtaatataaa ctcgctttct ttatatttac acttttagct
     7921 atatcatcaa gtgttgtacc gtcatacccc ttctctgaaa ataaggttat tgcgttatca
     7981 ataatcttat ccttcaattt ttataacccc ctactgaaaa ttaatcacac tatgttacag
     8041 gaaaattaag ttgcaattac aaatatttcc gtttaattat aacaacaatc tattgcaaat
     8101 taaaatacta tcaattacca tatggcttac aacctaacta acgaaaggta ggtaaagaaa
     8161 ttgcaatttt ttaacttttt gcttttttat cctgtattta tgtctattta ctggattgtc
     8221 ggttcaattt atttctattt taccagagaa attagatatt cattgaacaa gaagcctgac
     8281 ataaatgtgg atgaattaga aggcattaca tttttacttg cctgttataa cgaaagtgaa
     8341 acgattgaag atacgttgtc taatgttctt gcactcaaat acgagaagaa agaaattatt
     8401 atcattaatg atggaagttc agataataca gcagaactca tctataaaat caaagaaaat
     8461 aatgacttta ttttcgtcga tttacaagaa aacagaggta aagccaacgc actcaatcaa
     8521 ggcattaaac aggcttcata tgattatgta atgtgcttgg atgcagatac tatcgttgat
     8581 caagatgcac catattatat gattgagaat ttcaaacatg atccaaaact tggtgcagtt
     8641 acaggtaatc ctagaattcg aaataagagt tctattttag gtaaaattca aacgatagaa
     8701 tatgcaagtt taattggctg tattaagcga agtcagacac ttgctggcgc agtcaatact
     8761 atttcgggtg tcttcactct atttaaaaaa agtgcagttg tcgacgttgg ctactgggat
     8821 actgatatga ttaccgaaga tattgcagtt tcttggaaat tgcatttacg tggatatcgt
     8881 attaagtatg aaccgcttgc catgtgttgg atgttggttc cagaaacatt gggaggtctt
     8941 tggaagcaac gcgtgagatg ggctcaaggg ggacacgaag tattactacg agactttttt
     9001 agcacaatga aaacgaaaag gtttccttta tatattttga tgtttgagca aatcatctcg
     9061 attttatggg tatatatagt gcttctatat ttaggctatt tgttcataac agcaaacttc
     9121 ttagactata catttatgac atatagtttt tcaatatttc tactatcatc atttactatg
     9181 acttttataa acgttattca atttacagtc gcactcttta ttgatagtcg ctacgagaaa
     9241 aagaatatgg ctggactcat atttgtaagt tggtatccga cagtatactg gattattaac
     9301 gcagcagtag ttcttgtcgc atttccaaaa gcattaaaac gtaagaaagg tggttacgca
     9361 acatggtcaa gcccagacag agggaatacc caacgctaaa atcatcgcta aatattgtaa
     9421 gagaaacagc acttatcgct atatcttgtg tcttttggat atattgttta gttgttctac
     9481 tcgtttatat tggtactata tttgaaattc atgacgaaag tatcaataca atacgtgttg
     9541 ctttaaacat tgaaaatact gaaattttag atatatttga aactatgggc attttcgcga
     9601 ttatcatttt tgtatttttt acaattagca tattgattca aaaatggcag agaggaagag
     9661 aatcgtgaag tatagaaaat ttataatttt agtgttgagt atcttgatca tattgcctgt
     9721 aagcacactg gatggtcatc atattgcaaa tgcagatgac gattcaccta aaaaactgaa
     9781 atataaagaa aatagtgctc tggcattaaa ttatcaccgt gtaagaaaag cgaattttct
     9841 gaataatttt atttacttct tttctagtag taaagaaatt aaaaattata gtgttagtca
     9901 atcacaattt gaatctcaaa taaaatggct aaaatcacat gatgctaaat ttttaacctt
     9961 gaaagaattt ttatattaca agaaaaaagg taagtttcca aaacgaagtg tatggattaa
    10021 ctttgatgat atggatgaaa ctatttatga aaatgcttat ccaatcttaa aaaaatataa
    10081 aataccggca actgggttta ttatcacagg tcatgttggg gaagaaaact ttcacaacct
    10141 cgatatgatt agtaaaaaag aactaaaaga aatgtataaa actgggttat gggaatttga
    10201 aacacatacc cacgatttgc ataacttatc taaaaataat aagtcaaaat taatgaaagc
    10261 ttctgaagct acaatcataa aagatttaaa caaaagtgaa aaatatctaa ctaaaaactt
    10321 taaaaagtcg cagaaaacta tagcctatcc ttatggcttg atgaatgacg ataaattacc
    10381 ggtaatcaaa aaagctgggt taaaatacgg tttttcatta gaggaaaaag cagtcactcc
    10441 gaactccaat gattattaca tccctagaat attaattagt gatgatgctt ttgagcattt
    10501 aattaagaga tgggacggat tccatgaaaa agattagact tgaactcgta tatttacgtg
    10561 ctattatatg tgcaattatt attatcacac atttacttac acaaattact ttaaaacatg
    10621 aaaatatgga gggtggatcc ttagtgttac aattttacat tcgtaatatt gtgatttttg
    10681 gtacaccttg ctttattatc ttgtcacagt tactgacaac cttgaattac caaaaagtca
    10741 cctatagata cttaactaca cgcgtaaaat atatacttat tccttacata ttaatgggat
    10801 tgttttacag tta
//
LOCUS       DAMQYT010000074        10786 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMQYT010000074 DAMQYT010000000
VERSION     DAMQYT010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10786
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.074"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(25..540)
                     /locus_tag="PGA33_001450"
     CDS             complement(25..540)
                     /locus_tag="PGA33_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6473267.1"
                     /translation="MAGIIKEISEQNASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            731..1735
                     /locus_tag="PGA33_001451"
     CDS             731..1735
                     /locus_tag="PGA33_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDG6473268.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            complement(1781..2182)
                     /locus_tag="PGA33_001452"
     CDS             complement(1781..2182)
                     /locus_tag="PGA33_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6473269.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVLKSYQGQGYGSLIMEHIMQYIKGVAVESTYV
                     SLIADYPADKLYTKFGFIPTEPDSGGMYIKY"
     gene            complement(2218..3252)
                     /locus_tag="PGA33_001453"
     CDS             complement(2218..3252)
                     /locus_tag="PGA33_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDG6473270.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDRHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            complement(3263..3379)
                     /locus_tag="PGA33_001454"
     CDS             complement(3263..3379)
                     /locus_tag="PGA33_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473271.1"
                     /translation="MANYAIYFNNININSIMALNDNNYQFEHVNERNEYRES"
     gene            complement(3484..3915)
                     /locus_tag="PGA33_001455"
     CDS             complement(3484..3915)
                     /locus_tag="PGA33_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HDG6473272.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            complement(4122..5399)
                     /locus_tag="PGA33_001456"
     CDS             complement(4122..5399)
                     /locus_tag="PGA33_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDG6473273.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGSFVYIYFMQGVQRFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKS
                     VPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNST
                     MWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS"
     gene            5560..6183
                     /locus_tag="PGA33_001457"
     CDS             5560..6183
                     /locus_tag="PGA33_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDG6473274.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGVPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
     gene            6229..7173
                     /locus_tag="PGA33_001458"
     CDS             6229..7173
                     /locus_tag="PGA33_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDG6473275.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(7259..8701)
                     /locus_tag="PGA33_001459"
     CDS             complement(7259..8701)
                     /locus_tag="PGA33_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HDG6473276.1"
                     /translation="MNYKQSAEEILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            8884..9267
                     /locus_tag="PGA33_001460"
     CDS             8884..9267
                     /locus_tag="PGA33_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDG6473277.1"
                     /translation="MKERGYIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDVALLIEPSIGSVCNAFDHMWGYLKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
BASE COUNT         3614 a         1875 c         1571 g         3726 t
ORIGIN      
        1 ttttatctgc tgcaaaaaca atcattataa ctctttttcc ataattaaat ctgtatccgt
       61 tacatcacct gtttgaaaat gatgttcacc aaccacttta aatccatgac gtttataaaa
      121 tgcttgagca cgaggattat gctcccaaac tcctagccaa attttatgtt tattatgttc
      181 ttgagcaatt ttttcggcca attctatcaa ttgtgaacct cttccgccac cttgaaagtc
      241 tttcaaaaaa tatatgcgct gcacttctaa ataggtctcc cccatttctt cagtttgagc
      301 actattaata ttcatcttta tataaccaac attcgcacca tcttcttgat aaaaataatg
      361 aaatgaatct acatggttaa tctcttgtgt aaatttctct acagtataat tgtctttaaa
      421 aaattgatca aaatctttgt catcatagta agaaccaaac gtgtcataaa atgttctagt
      481 tgctaattca actaattcac tagcattttg ttctgaaatt tctttgatta tcccagccat
      541 ataaatcctc caataaacag tgatcgaatc aaaatattac ttatgttatt tttcagccaa
      601 aactatttaa aaatacatta acacaaatca attacaaatt gtattgattg tgtgtaacat
      661 caataaatga tacatttatt ccagtaaaat ggccgtattt tcaaaagaga aaaagagagg
      721 atgtatcgtt gtgatagaaa catttaaagc gtttgtaatt gataaagatg agagtggtaa
      781 agtgacacca actttcaaac aattatcgcc tactgattta cctaaaggag atgtgctgat
      841 taaagtacat tactctggta taaattataa agatgcttta gcgactcaag atcataatgc
      901 agtcgtaaaa tcgtatccta tgattccagg aatagattta gctggaacaa ttgttgaatc
      961 cgaagcacca ggctttgaaa aaggagaaca agtaattgta acgagttatg acctaggtgt
     1021 cagccattat ggcggtttta gtgaatatgc gcgtgtaaaa tcagaatgga ttatcaagct
     1081 tcctgatact ttaacattag aagaatcaat gatatatggc acagctggtt atactgccgg
     1141 tttagcaatt gaaagacttg aaaaagttgg aatgaatatt gaagatggtc ctgtactcgt
     1201 tcgcggtgct tcaggtggtg tcggtacttt agcagtactc atgcttaatg aacttggtta
     1261 taaagttatc gcaagtacag gtaaacaaga tgttagcgat caattacttg aacttggtgc
     1321 caaagaagtt atcgatcgac ttcctgttga agatgatcat aaaaagccac tcgcatcatc
     1381 aacttggcaa gcttgtgtag accctgttgg tggcgaaggt attaattatg ttacaaagcg
     1441 tttaaatcat agtgggtcaa ttgcagttat tggtatgact gccggtaata cttatactaa
     1501 ttctgtattc cctcacattt taagaggtgt aaacatttta ggaattgact cggtatttac
     1561 tgctatgaaa ttaagacagc gcgtttggcg tcgtctcgca aaagatttaa tgcctgaaaa
     1621 tttacatgag atcaagcaag ttattacatt tgatgaactt ccagaacaac ttaacaaagt
     1681 aattaaacat gaaaataaag ggcgcattgt tatcgatttc ggtgtagata aatagtattc
     1741 atgaaaaaga catcccgtta tgcgagatgt cttttttaat ttagtatttg atatacatac
     1801 cgcctgaatc tggttcggta ggtataaatc caaattttgt atataattta tccgctgggt
     1861 agtctgcaat cagactaacg tatgtactct caacagccac acctttaata tattgcataa
     1921 tatgctccat aattagactg ccgtaacctt gaccttggta acttttcaaa actgcaatat
     1981 caacaatttg aaaaacagtt ccgccatcgc caatcactct acccatacca attaaccgat
     2041 ctttatcata caaggttact gtaaataagg cattaggtaa tcctttttca gctgcttcgc
     2101 gcgtctttgg actcatacct gcgttaatcc ttaatgcgca ataatcctcg caagtcggaa
     2161 tatcatatgt cactttaacc attatttacc ccacttttca tcacacaata tatcaaccta
     2221 gtataaatgt ttatttacaa tagtcttatt cgcttcttta aacacttcat gatgacttga
     2281 aacataaccc tctgcattcg catctggttg gatatatgtt ttagcaaggt tcgctgcatt
     2341 tgcaccatca ctaaatgcac ttgcaattag atgtgatttt gcatcatgat aaacaatatc
     2401 tccacacgca tagataccag gtatactagt tgtcgtatta ccaaatcctt taacacgaca
     2461 atcatcatgc atatctagct ttgaagatgt ttcactcaat aatgtattac aacgatcaaa
     2521 cccatgacta ataatgacat cgtcaaattt aactgtatgc ctatcgccac tttcaacatg
     2581 ttccaaaaca acttcactta tatgcgtttc atcatcattg ccgaccaagt atttaatacg
     2641 tgtttttggg catagtttca catttaaatc tgtcaccaac gttttcatcg cttcatgacc
     2701 acttacatct tcttttcgat aaacaactgt cacgctttta gcaatcttgg caatatcatg
     2761 cgcccaatct aatgctgtat ttcctccacc tgatattaat acatctttat ctttgaaacg
     2821 tctgtaactt tgtacaacat aatgtaaatt agttaattga tatctctcta cacctttaac
     2881 atctaattgt tttggattaa taatacccgc accaattgca atgataactg ctttcgatgt
     2941 atatatttct cccgcttctg tttcaacttc gaaatgacgt tctgcctttt tcctaatatc
     3001 taccacacgt tcattcaaat gaacttccgg tttaaaatat aatccttgct taattgtatc
     3061 ttttaaaatt tcatgacaag gttttggcgc aatgccgcca atatcccaaa taattttttc
     3121 agggtaaatt ctcatcttac cccctaattc agattgaaca tctatcaatc ttacagacat
     3181 atctcgcaat ccagcataaa agcttgcata caaaccagac ggaccgccac caatgattgt
     3241 aacatctttc attatgtgcc tcctatgact ctctatattc atttctttca ttaacgtgct
     3301 caaattgata attattatca tttaaagcca ttatactatt aatatttata ttgttaaaat
     3361 aaatcgcata gttagccatg aattatcaat aactatccca tcacaatata aaaacacacc
     3421 tttcaaagta agaaacaaca atgttccttt gaaagatgtg tatatttttt agttctagtt
     3481 atattatttt ttaaaagact catcacgtgg ttctttaaga attgcttgtc ttaaaaggaa
     3541 aaatagcaac aataaacctg caagcatacc tgtgtgccca atacctgcaa agcctgcaaa
     3601 tgcttctgga gagtatgatt taccagtgac ttggaagaat ccttttgtca ccatcatacc
     3661 tatagtaacg ataacaccta tgttatatac atagaagaac cagttaaata agtagtaact
     3721 tgataattta aataacttct ccaatggtaa taatattaaa aacataaaca tacctaaaat
     3781 aagtgtatgt gtgtgtacaa gtgataattg tgttgtacca gtaaaatcat atgcttttgt
     3841 tagctctcta tagaaaaagc cactaaataa acctatgatc atataaaaaa gaaatgaata
     3901 taataaccta cgcattttga atcctccctc accttttcat tatatccgat tatacctatt
     3961 ataacagtga caaaaaagcg aagatatgac acaactgaat ttggtttagc acatagtatg
     4021 acagctaaaa taactttaca tcaagcgcta tacaaataaa atagctatac catgacgctc
     4081 gctacaaacg tcttgtatag ctattatttc tatttattaa attatgacgg cacttcagta
     4141 cgttttgtgc tatgttttct aacaatagct gaaactaaac ttgatacagc gccaaaaatc
     4201 ataaacccta taaacatcca cattgtacta tttaattcaa tatgatgatt caagtataat
     4261 agttctctta cacttgttgc atagtgtgtg aatggattcc aatcatatac atatttttga
     4321 taactttcag gcaacatttg tttaggtaac gttacaagtt gcatactaaa gaacattaaa
     4381 atgaagaaaa ttggaactga cttcatacct agccatacca taacacctaa aataaggccc
     4441 acgaaaccaa gaatcgcaaa tgctacaaaa attgcgatac gatttggatg gtcaaaatca
     4501 aatctttgaa cgccttgcat gaaataaata taaacaaagc tacctgcaaa tgctgcaaca
     4561 actgcaaata tcatctgtcc aattgaagca atgatacgat gttgcacgac gatattgtta
     4621 ctagttctaa atgcaaagaa caataagata gacgttacga ttgaacctat ccaaattggc
     4681 ataaacatta ggaatggtgc attaccacct gcttggtgat ctttaacttt attaagtttt
     4741 tcattatcca cttttactgg attcgtcaaa ccattgatgt ccgcggcatt gactttaaca
     4801 ttttgactcg ttaatgtttc caaactttgc ttcgtaattt gagcattaat attatcaccc
     4861 ataccagtta acacagctga tgcaatttgt gaaccttgta agcttgatcc ttcacttaca
     4921 atcgttttaa atttagcctg cttaacctct acttgttgat tccccatttt ttgtttcatt
     4981 tgttgaacca cttgtggcgg aatttcacct gaagcaactt tttgttgcat ttcttctttt
     5041 ttactatcca taactacttt ttgtgtttta ctcatagcat cttttgaaaa gtctttatca
     5101 ataatagcta ctccaaagat tttttgatct ttcaaatctt tttcaaggtc tttttcacta
     5161 tcaaccttaa cccatttaat tttattagaa tcactatcta ataatttatc ctctaatttt
     5221 ttaccaatgt taacattttt atcttgaatc gttgtacctt tatcctcgtt taatatacca
     5281 attggtaaat cttttggttt aggattatat gcaggataaa aagctaaaga aaagattacc
     5341 aagataatca tagttgctat tggtgcaatc cataataatt tatttttaaa tatattcatt
     5401 ttattaatta cctccttatt agacaatgtg ttgaatattt cgcatgttgt ctattataat
     5461 atgtaagtgg gaataaacaa tacacaatgt tcatcaatac gttcattaat agacaaatac
     5521 acccacattg ttcaataaca caaggaggtt gatttatttt tgaaagaaga taggcgaatt
     5581 agaaagacaa agtcctccat taaacaagcg ttcactaaat tactacaaga aaaagattta
     5641 gaaaagatta ccattcgcga cataacaaca cgcgctgata tcaatagagg gacattttac
     5701 ttacattacg aggataaata tatgttactc gcagatatgg aagatgagta tatttcagaa
     5761 ctaacgacat atactcaatt tgatttgtta cgcggttctt caattgaaga cattgcgaat
     5821 acttttgtaa ataatatact caaaaatatt ttccaacata ttcatgataa tttagagttt
     5881 tatcatacta ttttacaatt ggaacgcacg agtcaattag aattgaaaat caacgaacat
     5941 attaaaaata atatgcaacg ttacattagt atcaatcatt ctatcggagg cgttccagag
     6001 atgtattttt acagttacgt ttccggagca acaatttcaa ttattaaata ctgggtaatg
     6061 gacaaacagc ccatttcagt tgatgaatta gctaaacatg tgcataatat tatttttaat
     6121 ggtccattaa gaataatggc agaaaaccgt ttgcataaat caaaccttga ttcactaact
     6181 taagtaatac tcgcttttcc taaatccttt agaaaggaac tatatatcat gattacttca
     6241 ttcagacatt ctgaagatat agataaacat attataaaaa caccattaga tcatacagcg
     6301 tcatggatta atgtagtaga accagaccga gaagaaattg aaaatcttat ggaacaatat
     6361 aatatacctg aagactttat acgtgaccct ttggactcag aagaaagttc ccgtattgaa
     6421 tatgacgaag atactggtta ctcattaatc attattgatt tacctatcgt caattcaact
     6481 aatcgtagcg ttctatcttt tgtaacgatt ccattaggca ttattattgg caacggtatt
     6541 atcgtaacag tttgtgacgc tgaaaatgaa tttttagaaa atttacctaa gcgtgatatt
     6601 aatttaaaat ttcacagcag atttgcatta gaaattttaa ctactattgc cgaccattat
     6661 aatcgtaact tacgattact taataaaagt agaattcgta ttgagaaaga actaaaaaat
     6721 aacattacta acaaacaact tttcaaatta atggaagttg aaaaaagttt agtatacttt
     6781 ttagctgcct taaaaggtaa cgatacaatt attaaaaagt tattccgttt acctgcaatt
     6841 aaacgttttg aagaagatga agaattacta gaagatttaa tcattgaaaa taaccaagcc
     6901 atcgaaacaa cagaattaca tcaacgtatc ttagaaagta tcacaacatc atacgcttcc
     6961 ttattatcta atgatatgaa tacgattatg aagacattaa cacttttcac ggtactatta
     7021 acgttaccaa cactcgtatt tagtttcttc ggtatgaatg tgccgttgcc aattgatgac
     7081 catagttata tatcttggat tattgtggta ggtatttcat taattcttgt cgtaattgtt
     7141 agtattttct tatggcgtaa acagaaatta taactaaatt aaaaggtatg acagcttaaa
     7201 gtttcatcct attgcatttg caatgactga tactttgatt actgtcatac ctcttttttt
     7261 attctacaac ttctttattt gcttttcttt tagataaaat taaagtaact gttatagcaa
     7321 tgatgaatga tattgtcata ccaacaaagt agtgtaacca tcctgcatgt actggattga
     7381 ttgaaataaa tcctggcaat ccagcagttc ctaatgcgat tgctttaacc ttgaagaaag
     7441 caatatatgc tgaaccaata cctgatccaa cgatagcgcc aataaatgga tatcttagtt
     7501 ttaagttaac accaaacata gccggttctg taataccaag taatgctgaa atacctgcgg
     7561 cagatgccac accttttaac ttcttatttt gtttaataat aaagaacgct gcaattgctg
     7621 caccaccttg tgcaacatta gacattgtcg caatcgggaa tataaatgat ccacccgttt
     7681 tagtggcatc tgcaattaat gtcgtttcta cagctataaa gctatgatgc atacctgtaa
     7741 taacaatcgg agcatacaat aatccgaata ttaatccacc aattgcacca ccaaattcat
     7801 aaagccatgt taaaccatct gataaccagt aacctaattg tcgagtgatt ggacctacaa
     7861 atgaaaatgt tagaaatgct gttataaaaa tagataacaa tggtgttaac aaattatcta
     7921 acaccgttgg aataactttg cgtaaccctt tttcaattga ggctaagata taagctgcta
     7981 caagcatagg taacacttgt ccttgataac ctacttgatt aatatgcaaa ccaaaaacat
     8041 cccaatatgg aatagcctta cctgcttcaa ctgcttttgg gaaatcgtat gcgctcatta
     8101 gcgatggatg aactagtatc atacctaatg cagcacctaa aaatggattg ccaccaaagc
     8161 gttttgctgc actaaatcca attaaaattg gtaataatgt aaatggtgca ttcgcaaaaa
     8221 catttatcat ttcagctaat ccagcaaatt gactatatac atctatcaat gatttacctg
     8281 aaaagaataa atctttcgca gtcaaaatgt tatttaaccc cattaataaa ccaccagcaa
     8341 cgatggctgg tataatcgga acaaagatat ctgaaagcat ttttacaaat cgctgtaacg
     8401 gattcatatt tttagcagat tgtgctttga cttccgaagt ggttgatgct tcttttccag
     8461 ttaatttttc cagttcacta aatactttat tgactgtacc agacccaata ataatttggt
     8521 attgtccccc agtagaaaac gtccctttaa ctacatccat attgtttagc gcctcttcat
     8581 ttactaaact ttcatcattt aaaactaatc gtagtctcgt tgcacaatgc gccattgcat
     8641 ctaaattctc ttctccgcct atcgcgttca aaatttcttc tgcggattgc ttataattca
     8701 tatatttagc ctcctaatta gaaccggttc catttattcc actttattgt aaccggttcc
     8761 aaaaatattt gcaacactct attatcattt tatataaaca cctacaccat ttttgatagc
     8821 gcattcattt ttattgctat actacatgca tgtatattaa cattaattat cggaggtaat
     8881 tttatgaaag aacgtggcta tatcgaacag ctttggcgtg aggaaaaata tcatgtattg
     8941 ttacacagtc aacagtctta ccaaatgata aggaatgctt taaaaacgga tttatcactt
     9001 catcaagttc aacaaatgat tgatgttgct ttgttaattg aaccgagtat aggaagtgta
     9061 tgtaatgctt ttgatcatat gtggggatat ttaaaaaaat gtgcgaatga agaagaaaga
     9121 caacaatcaa aactacttaa agctgatttt attaatggta aaatcgacac ccaaacttta
     9181 ttagattttt tagcagaact tgctaataaa tatgatgttc aatatttact acaaagtcgt
     9241 gttttaaaca caaaaagaaa gagatagatt gaaactttag ttgtaacgga caaatagtag
     9301 tcattggagt tcagcaattc atactttgta ctaaagttta ctataatcta tctctcaagg
     9361 tttaaatgta catatctata ttaaaatgcg tttcatgatg ttagaagata ccatacacat
     9421 ttaattagtt acagcataaa tcaatttata cccttaatta ttacattgtt tatattttaa
     9481 attcggggga gtaattttaa gtaatatctt gttgctgcta attctattgt taataaattg
     9541 atattagatg tagaaattga gtgtgaaagt taataataga tgatttagct agtaacataa
     9601 ttaattcata tatagtcaaa aagttctgtt agatactgtt cttgatgaat attctttgat
     9661 gttaggtgtt agctattcta gatgagtttc ttagatgtgg atgcctgtct tgatgaagtt
     9721 atctatgatg ttaatataat acttgatgtt gattcttaga tgttagtgcg atttcaagat
     9781 gctgattctt tgttgttgca ctatcttggt gacgtttcta gttgtttata gttatcgcat
     9841 ctcttgatgt aaaatctaag atgtttatgc atttctagat gttaattctt cgctgcttaa
     9901 gaattatctt gatggtaaat ctagttgtgc catgcatttt ctcatgatga atctaaggtg
     9961 ttaattcatt gatgtgtgct gtttcttgtt gcttcattat cttgatggtg aatttcgttg
    10021 tctaatgcac tttttcaaat gattattcta attgttacag caataactag atggattttc
    10081 ttagatgttc acttttctag atgtttatac ttatcttgat gttttctaaa cagaactttt
    10141 aaacgcttta ttttttagta gattgtttaa aatttcttag ttgatggtta ttgttgttga
    10201 tttgaataat ttgtagcgaa tattttgtaa cagggctact aagaattttt ataacaagaa
    10261 gtgatttcaa tcgtactaat tttcagcgtt tgatgacaaa agtgtatgtc caaacttaaa
    10321 taatggcgat aaaatttaag tcatgggtac atttaatgtt ttttgattca aacttccatt
    10381 ttggtcaatt tgcttttttt ataacatagt tgaatatcaa taaaagatta gcaagttagt
    10441 tcaaaatggc tatgtattgt tttttgaaat acataggttt gaaatctata ttaattatgc
    10501 accaaataat ttaattagac tctatcgaaa atttccaaat tttgcttact atctttctaa
    10561 ttttactttt atattgttcg caatgcataa aaacaagcgt tttcctagga gttttcatta
    10621 atttctttta tttttcacta ttaccttttc aaaaatattc aactataata ggtatactat
    10681 ccatgatttt aaaaatttcc atgttttaca tttaaatgat agatttcaat atatattagg
    10741 caggagagag aaatatgaca tgccaactta aaatacatca tacatt
//
LOCUS       DAMQYT010000075        10695 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMQYT010000075 DAMQYT010000000
VERSION     DAMQYT010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10695
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.075"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(90..178)
                     /locus_tag="PGA33_001461"
     tRNA            complement(90..178)
                     /locus_tag="PGA33_001461"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(141..143),aa:Ser,seq:gga)
     gene            complement(233..304)
                     /locus_tag="PGA33_001462"
     tRNA            complement(233..304)
                     /locus_tag="PGA33_001462"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(269..271),aa:Glu,seq:ttc)
     gene            complement(306..380)
                     /locus_tag="PGA33_001463"
     tRNA            complement(306..380)
                     /locus_tag="PGA33_001463"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(346..348),aa:Asn,seq:gtt)
     gene            complement(395..468)
                     /locus_tag="PGA33_001464"
     tRNA            complement(395..468)
                     /locus_tag="PGA33_001464"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(434..436),aa:Gly,seq:tcc)
     gene            complement(484..559)
                     /locus_tag="PGA33_001465"
     tRNA            complement(484..559)
                     /locus_tag="PGA33_001465"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(524..526),aa:His,seq:gtg)
     gene            complement(573..645)
                     /locus_tag="PGA33_001466"
     tRNA            complement(573..645)
                     /locus_tag="PGA33_001466"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(610..612),aa:Phe,seq:gaa)
     gene            complement(662..737)
                     /locus_tag="PGA33_001467"
     tRNA            complement(662..737)
                     /locus_tag="PGA33_001467"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(702..704),aa:Asp,seq:gtc)
     gene            complement(759..832)
                     /locus_tag="PGA33_001468"
     tRNA            complement(759..832)
                     /locus_tag="PGA33_001468"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(796..798),aa:Met,seq:cat)
     gene            complement(1304..1858)
                     /locus_tag="PGA33_001469"
     CDS             complement(1304..1858)
                     /locus_tag="PGA33_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDG6473278.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            complement(2181..3581)
                     /locus_tag="PGA33_001470"
     CDS             complement(2181..3581)
                     /locus_tag="PGA33_001470"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HDG6473279.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGFDLLKKPTQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYNDQLEEVY
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTHDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            complement(3605..4528)
                     /gene="hemH"
                     /locus_tag="PGA33_001471"
     CDS             complement(3605..4528)
                     /gene="hemH"
                     /locus_tag="PGA33_001471"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDG6473280.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            complement(4586..5623)
                     /gene="hemE"
                     /locus_tag="PGA33_001472"
     CDS             complement(4586..5623)
                     /gene="hemE"
                     /locus_tag="PGA33_001472"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDG6473281.1"
                     /translation="MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            complement(5613..5723)
                     /locus_tag="PGA33_001473"
     CDS             complement(5613..5723)
                     /locus_tag="PGA33_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473282.1"
                     /translation="MIKCQFYDIYLLKIRTNERYDNIDKNDFNLGGLYGA"
     gene            5886..6389
                     /gene="traP"
                     /locus_tag="PGA33_001474"
     CDS             5886..6389
                     /gene="traP"
                     /locus_tag="PGA33_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HDG6473283.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPSIYEVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            complement(6513..7736)
                     /locus_tag="PGA33_001475"
     CDS             complement(6513..7736)
                     /locus_tag="PGA33_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6473284.1"
                     /translation="MRNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFITFALTYYLLLAPKWYIAFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(7729..8469)
                     /locus_tag="PGA33_001476"
     CDS             complement(7729..8469)
                     /locus_tag="PGA33_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6473285.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDETMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            8603..9025
                     /locus_tag="PGA33_001477"
     CDS             8603..9025
                     /locus_tag="PGA33_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDG6473286.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            9167..9532
                     /locus_tag="PGA33_001478"
     CDS             9167..9532
                     /locus_tag="PGA33_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDG6473287.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            10265..>10695
                     /locus_tag="PGA33_001479"
     CDS             10265..>10695
                     /locus_tag="PGA33_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HDG6473288.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALN"
BASE COUNT         3481 a         2000 c         1560 g         3654 t
ORIGIN      
        1 gataatagct caaatgcaga tatatcaagg gattgagtcc aaaataaaaa tagcgcttaa
       61 aatgggtaaa accaatttaa gcgcctttac ggaaggtaag ggattcgaac ccttgagacg
      121 cttgtgacgc ctacacactt tccaggcgtg ctccttcggc cagctcggac aaccttcctc
      181 aacaataaat atgtaataaa aaaacagaag cgatatttca cttctgcttg tatgactcct
      241 acgggactcg aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaagg
      301 agccatggct ccacaggtag gactcgaacc tacgaccgat cggttaacag ccgatagctc
      361 taccactgag ctactgtgga ataatagaaa ataatggagc gggtgatggg aatcgaaccc
      421 acaacatcag cttggaaggc tgaggttttg ccattaaact acacccgcta atgtaaacga
      481 ttatggggcg gctgaagggg atcgaaccct cgaatgtcgg aaccacaatc cgatgtgtta
      541 accacttcac cacaaccgcc atgaattaag aatggttcag gacagagtcg aactgccgac
      601 acatggagct tcaatccatt gctctaccaa ctgagctact gaaccgtaat ggtaataata
      661 atggcggtct cgacgggaat cgaacccgcg atctcctgcg tgacaggcag gcgtgttaac
      721 cgctacacta cgagacctat taaattaaaa actatgtatt gcgggaggcg gatttgaacc
      781 accgaccttc gggttatgag cccgacgagc taccgaactg ctctatcccg cgctaatatt
      841 attttgaatt acctaattaa tataccataa tcaaaaacct aaagtcaaga actttttgaa
      901 tttaatttaa atgttatctc ttaaataatt acttaaatat cgtagcaaca tgttctctgt
      961 tgaacacaaa tattagtata ttcatttttg tagtgtacgt caacgacatt ttcaaagttt
     1021 tttgtgtaaa aaaacgcttc ttattccctt ttatcatata agtgtctaat agttgtcata
     1081 aatagtgtta aagcatttaa aaaggtatag gagttataaa gtttacaacg cctatacctt
     1141 ctgaaaaaga aattataaaa gcttgttaca ccgcatattc tttcagtcag cgactaccaa
     1201 tataacattg tagccctaag acattgcttg acgcctcaat tacaacaatt tttcaaaatc
     1261 agcagctacc tactgacaca acataacaca acccatacac taactatcgt gtcatgtaat
     1321 cttgcatccg atcttgcaac gctgtaaatg tttcgaagcc atcctcttct aagaagtgcc
     1381 ctccatcttc cacgattcgc aagttcccct ctaatgcatt cattaaacgc tgggtctctt
     1441 tatatgaaac gtatttgtca tttttagaac tcaatccgta aaaattgtca actttctttt
     1501 taatattatc gtaatcaatg gttacattac ttaaatcaat atctaaatct atattttctg
     1561 catcttcttt aaagcccgct atactgaaaa agccttcaat cggctgatca atcatttcaa
     1621 tatattttaa agctgtgatt gaacctaaac catgtgttac aaaatatgta tcctttttgc
     1681 gtacattaat ttgtttcgtc atagcttcaa tccactgatc cactgtcttc gcttcagggg
     1741 attcaaaatt aaataatgtt acgtcatatc cttctaaagt taagttatgc tccaaccact
     1801 gataccaatg atttctacta tttccatgca tagaatgtac aataattaca tctgtcatct
     1861 cattctctcc tttcaactta ctacttcttt tctattttta aaaaaatgac tgattaccta
     1921 taattgtaaa ataaaaacac cttaattaga aatgttatat cgcaaagtga catttctaat
     1981 taaagtgtat tgtcatcatt tcaatatcat tcaaaaacag ctaaaccttt gtctctgctt
     2041 caatttcaca aaaataattc ccgctgaaag tatctatatt tacacattac ttccaccatt
     2101 atataactta aaaatgacta tatttcatca aacattatct aaaggcgtcg cacctacacc
     2161 aacaccatcc aacaattaac ttacaactct gcgattactt cttcagcagc aactttacct
     2221 tgcgtaatac aatcaggtag tccaaccgct tcaaaagatg caccagttac tctaagtcgt
     2281 ggatatgttt gtttaatatg tgcttgaatc tgtctaattt gttgaatatg accgacatgg
     2341 tactgtggca tacttttcgg caaacgattg acaattgtaa attcaggatc acctttaaat
     2401 gtcatcattt gacttaaatc tctacgtaca atcgatacta attcattatc tgtatgatca
     2461 tcaaccacag tatcacctgg tttacctaca tacgcacgaa tcaaaacctt accttccggt
     2521 gtagtaaatg gccatttttt cgatgtccaa gtacatgcgg taatgtctgt atcactcgtt
     2581 ctcgcaatta cgaagccagt accatcatgg gtattttcaa tgtctttttc atcaaatgcc
     2641 aatacaacag ttgcaacagt cgtactatcc atcgttttaa agtaatcaaa tgctggatct
     2701 tgtccgaacc aatttaaaaa cacttgatgt ggtgtcgtta ctaatacgcc atcatacact
     2761 tcttctagtt gatcattgta aacaatttta tattgttttt gagatgtaat tatatcatcc
     2821 actgacgtat tgtagcgtat tgtcacacct ttatttttaa catcttgttc taatgcttca
     2881 ataaatgagc ttaaaccatg cttaaattgt ttgaattgtc ctttcggtgc gccaggatat
     2941 aattgtcttt gtttcagacg cttatttttc tcatccttca taccttttat cagacttccg
     3001 aatgcctctt ctttttcttt aaaattagga aacgtactca tcaaacttaa tttatcaata
     3061 tcggtaccat aaataccacc cattaaaggc tctattaaat tctcaagtac ctcattacct
     3121 aatcttgctc tgaaaaatgc accaacagaa atgtcaccat cctgcatttg agtaggtttt
     3181 tttaataaat caaaccctgc tcttaattta ccaagtggcg atattaattt tgtagtaaca
     3241 aatggtttaa tatctgttgg aatacccata attgaaccac ctggaatcgg atataattta
     3301 tttttcgcaa aaatatatga ttgtccagtc gtatttgtaa caatatcttg ttctaatcca
     3361 atatctttcg ctaattctgt cataatcgtt tttctaccta aataagattc aggccctagt
     3421 tcaatcatat aaccatcttt acgatacgat tgaatctttc cccccggacg attcgatgct
     3481 tcaaagatgg ttacatcaat attaggatct tgctgtttta aaaaatatgc acttgataaa
     3541 cctgttatcc ccgctcctat aatagccact gatttagtca caacgtttca cgcttctttc
     3601 gtcattaaaa tatagacttg atttcatcaa caattgcacc gataaataat ggatgtgtat
     3661 tcggcatttt tggacgataa taattcgcac caatatcatc gcaaactact ttacattcat
     3721 aatcattgtc ataaagcacc tctaaatgct cacatacaaa acctactggc gtatatataa
     3781 agtttttata ctgatgtttt tcatataaat cacgtgttaa atcttgtaca tctggcccta
     3841 accaaggtgt acctgtatta ccttcagatt gccaaccaat cgcgatatgt tcaatattag
     3901 attgttcttt aattaaaagc gcagtatgtt ctagttcttg tggatatgga tcattattct
     3961 tttcgattaa accttttggc aaactatgtg ccgaaacaac taataccgtg tctttatgtt
     4021 cctcttccgg tatttgagct aatgtttcgt tgactttatt cgtccaatat tcaataaatt
     4081 taggttgttc ataataatgt ttcacatgtg taagttgaat accatatttt gcagcttctt
     4141 catcagcacg tttgtcatat gatcctactg aaaatgaaga ataatgtggt gctagtacta
     4201 ccgtgattgc ttcagtaatg ccatcattgt gcatttgttc aaccgcatct tcgataaatg
     4261 gtgaaatgtg ttttaatcct aagtatagtt taaattcaac atctgcatat gctttattta
     4321 atgctgaaac tagcgcatca gcctggtcat ctgttgtacc tgctaatggt gataaaccac
     4381 ctataaattc atatctatct ttcaaatctt gaagttcttc ttcagatgga cgtttaccat
     4441 gtctaatatc tgtataatat ggctctatgt cactttcttt ataaggtgtg ccataagcca
     4501 taactaataa tcccattttt ttagtcattg ataatacctt cctttaaatg aattatcttt
     4561 catgtgcttc aatgtaaaac tatgattatc tttgtgtata tgtgtgtacg aattcgctta
     4621 ctttacgtaa cgtctctggt tgcacttctg ggaatacacc gtgtcctaaa ttaaagatgt
     4681 gtttaccgtt ctccatacct tgatctaata ttggtttcaa tctctcttca atgacattcc
     4741 atggtgctaa taaaattgat ggatctaaat tcccttgtaa tgttttagta acgcctaatt
     4801 gttgagcctg attaatagac gttctccaat ctaggcctaa tacatcaatc ggtaaatcat
     4861 tccattcatt gattaaatga ctggcaccta caccgaataa aattaccggc acatcatgtt
     4921 tttctttaac ctcactgatt aatcgaatca tatgtggttt aatgtaacgt ctgtaatcct
     4981 cgacatttaa tgcacctacc catgaatcga aaatttgaat caattcggca cctgcttcga
     5041 cttgagctgt tacatattta acagatacat caactaaatg attcattaaa gcaaaccatg
     5101 ttgcttcatc tctatacatc atcgcttttg taaaattgta atttttcgat ggtccgcctt
     5161 caatcatata tgacgctaat gtaaatggtg ccccagtaaa tcctattagc ggcacattta
     5221 acttttcttc tgttaaaagt ttaattgtat ctaatacata tggtacatct cgttcggggt
     5281 ctatttgaga aagtttctca acatcttgaa ttgttttgat tggattatga atcactggac
     5341 caatacccga tttaatttct acatcgacac caattggctt taatggtgtc ataatatctt
     5401 tgtataaaat tgctgcatct gtatgataat tatcaactgg taaatgtgtt acataagcgc
     5461 acaactccgg ctgatgtgta atatcgaata gtgaatattt ttctttcaat tttcgatatt
     5521 ctggttgcga acggccagct tgtcgcataa accaaacagg tgtatgtgat gtttcttcac
     5581 ctttgatcat ttttaaaatt gtattgtttt tattatgcac cataaaggcc tcctaaatta
     5641 aaatcattct tatctatatt atcatatcgc tcattcgttc gtattttcaa taaataaatg
     5701 tcataaaact gacatttaat catagaacta tttattgtaa atttaaattc taaagtccat
     5761 tattttgtat cattacttct aaatatctcg caagattcat tatagtaatt ttaatcaatt
     5821 attaatagtg gtaatgacta gtttatcatc gtataataaa taaaaacata agggggacct
     5881 ttcatatgaa gaaactatat acatcttatg gcacttatgg atttttacat cagataaaaa
     5941 tcaataaccc gacccatcaa ctattccaat tttcagcatc agatacttca gttatttttg
     6001 aagaaactga tggtgagact gttttaaaat caccttcaat atatgaagtt attaaagaaa
     6061 ttggtgaatt cagtgaacat catttctatt gtgcaatctt cattccatca acagaagatc
     6121 atgcatatca acttgaaaag aaactgatta gtgtagacga taatttcaga aactttggtg
     6181 gctttaaaag ctatcgtttg ttaagacctg ctaaaggtac aacatacaaa atttatttcg
     6241 gatttgctga tcgacatgca tacgaagact ttaagcaatc tgatgccttt aatgaccatt
     6301 tttcaaaaga cgcattaagt cattactttg gttcaagcgg acaacattca agttattttg
     6361 aaagatatct atacccaata aaagaataga ttcgaagaaa acttccattg gttatgctgt
     6421 taacgtatag atgctaatgt aatctgaacg ccgtatcatt tggcgttcaa cattacatgc
     6481 acctttacaa catacatgcc aatggaagtt ttttagtctc gtaataatgt ttcctgatat
     6541 ttcaactttt taataatact tctaatcgtt aataaaccta caatatagaa aatgagtaca
     6601 acatagaata acgtcatatg ttttataata aatgtcactg caaacacagt acaaataaca
     6661 aacattaatc tatataaaaa ctgttcatac cctttgatta ccttctcttc aggtacaggc
     6721 cacacttgag gccataagcc atatgcttgc tgtgaataaa attgtgccat ttgtaacaat
     6781 ataatatata caaataaaca cccaataatt gctgtcacta atggatatga taaccaaacc
     6841 attaataaaa ctgcaataat tactaaccta aagataatat taaatgcgtc tctccctctt
     6901 ataaagcttc taataaataa gaataaatac atcgcattag agttaaattt actacccttt
     6961 ggaactggta aaagtatatc tagataactt cttctgactg cagattcttt caaatgtttt
     7021 acatcggtga acatattaac aaatttataa taattcatat gatgtcgatg ttcgattgca
     7081 atcattttct cccaaggata caaaaagcct ggtttatatt ttttaactaa aaattctatt
     7141 aacacaggca aagcaaccat cacaaatgcg atgtaccatt ttggagctaa tagtaagtaa
     7201 tatgttagag caaaggtgat gaatgatatt aaattaactt gccatgtttt aagtcccgat
     7261 tgataccatt gccatcttaa gcgtaaacca acatatggaa aaattaatgc actgactcca
     7321 aaacaaatat aaaatgccac attatgttga ttaatattgt aaaacaacgg gaacattaca
     7381 ataacaataa tgagttggat taatatgcgc gcaaagtaac tatataaaat cgcatgacgc
     7441 ataaattgag acatgtgttt ttcaaatggt aataaaaaga ttttatccgc ttcttttaac
     7501 agtggtcgca ttggaaaaat agatgtcaac gcaacaatca ctgctgctat taatgaaaaa
     7561 ttgatattcg ttggaatatg ttttaaccat tcaccatatc caaaaataaa tgcacccagc
     7621 aaaataagta aaaagaccat gaaatgacca ttaaatataa acttattata ataatttttc
     7681 tctttacgaa gggcatgtaa tcttttatta aataatgtgg tagcttggtt acgcatgtac
     7741 atctccacct tgcgtcacat gaatatatat atcgtctaat gtttgattat gtaagccagt
     7801 ttgttgtctc aatgcttcta aatctccaaa tgcaacgact tcaccttcgt ctagtatgat
     7861 aaaacgatca cagtaacgtt cagctgttgc taaaatatgt gtactcatta gaacggttct
     7921 accttcgttt ttcttttcaa ccattaaatc taacatggat tgaattccta atggatctag
     7981 gccaaggaat ggttcgtcta taatatacaa ttcgggatta acgataaacg cacaaataat
     8041 catgactttt tgtttcatcc ccttagaaaa atgactcgga aaaactttca actcattttc
     8101 taaacggaat gtctttaata atggcattgc tcgattcatc gtttcatcac gatcaatatc
     8161 atatgccatt gctgtcatct caatgtgttc ctctaatgtg agttcttcat aaataaccgg
     8221 tgattccgga atataagata actttcttct ataagcctct atgtcatcat taatgttgat
     8281 atctgaaatt gatagagatc cttccatagg tgtaagcaat cctagcatat gtttaatcgt
     8341 tgtactctta ccagcgccat taaggccaat aagtccaaca atttcgcctt tgtttaattc
     8401 aaaatttata tctttaatta cagggcgttt tccatatcca cctgtaagct gttctacttt
     8461 aactgtcata aggcacctcc atgacttata ttgtaccaaa aattataaaa tgctcatatt
     8521 aaatacacat gtcctaatat cgaattttta gcgacaatgt tataatgaat ggtaatacta
     8581 gttgaaaagg agtgtagtca tcatgtcaga aacaattttc ggcaaaattt taactggaga
     8641 aattcctagc tttaaagtat atgaagacga ttatgtctat gcctttttag atatatcaca
     8701 agttactaaa ggacatacgt tattaattcc taaaaaagct tctgctaata tctttgaaac
     8761 tgatgaagaa acaatgaaac atatcggtgc agcattacct aaagtagcaa atgctattaa
     8821 gcgtgcattt aatcctgatg gtttaaacat tattcaaaat aatggtgagt ttgcagatca
     8881 atctgtattt catattcatt tccacttaat tcctcgatac gaaaatgata ttgatggatt
     8941 tggttataag tgggaaacac atgaagacat tttagataac gatgcaaaac aacaaattgc
     9001 tgaacaaatt caagcacaat tttaaatgta tgcttaatct aagctcgaac gggtataata
     9061 tgattaatat tataacaatt gcgtttgaag tgataacatc aaggttagca attttaaaca
     9121 aaatgagtta tcaagataac agatgttaaa agtgaggaga atataaatga aagcatcacg
     9181 cattctattc ggtatcggtg ttggcgtagc agctggtttt gtagttgcac ttcaaggacg
     9241 tgacgacaaa agtgtcaaga acaacacgat cgatcgtact gcccctactg gttcaaaatc
     9301 agaactacaa cgtgaatttg aaacgattaa acaaagtttt aatgacattt taaactatgg
     9361 tgttcaaatt aaaaacgaaa gtgcggaatt tggtagttca attggtggtg aaattaagtc
     9421 attacttgga aacttcaaat ctgacattaa tcctaatatt gaacgtttac agtcacacat
     9481 cgaaaattta caaaatcgtg gcgaggatat tggaaacgaa atttctaagt agcaggttac
     9541 gttctcgatc acaactattt ttattagtaa cagcatattt attttttaaa attaaatgcc
     9601 aaataaacga gatgacatta gaaattagat atttcttgtc atctcttttt taaaactcaa
     9661 atgaacttat gtttacaaat tataggaaga cattgtttgt agtgattttc gcttaaatca
     9721 tatttatgaa ttgattgaaa acattgctta ggattcattg tgttatcctt gcactttgat
     9781 tacgctttac ttaaatcatt atcgacaaac aacatactta tattttcatt gagccgaacc
     9841 ttatatacac attacatata ccttacttgc acaaattatt aatctggtgt ttattataat
     9901 tacatatcac tatattttta gcatttgtat aacttagttg gtcaaaagat gcttttgcat
     9961 atgccctgcc atatccattt tttatattat tcaagttttc aaatactcat gtcacacgcg
    10021 cctttttcat aagtgatgct ttattagcaa gaatatgtgt tcgcagaaat ttgttctgca
    10081 ttctacttct acgctagtca atcagacaat tttaccaatc cccactttcg cgtttcaaat
    10141 caaacaatac gtcgctcctt tcttcttata taacaattct tctaacatga tatgttacta
    10201 ttgaattact gaacctgagt tagttataat ctaacttata ttgaaaagag atgaggcgta
    10261 agatatgttt ttatgtaaaa gacaaattga tatcaatgca cgatttggtt tgcctagaat
    10321 tgcatttatg agtgcagttg caaccatcat tatgttttta gttagttatg aagtaatgta
    10381 ttttttatct aatacgccat tatcagatag acattttctc atctttttat tacttgtatt
    10441 tatgacgtat ccattacata aaagtataca tttattattt ttcttaccat atagaaaatc
    10501 gtttaaagtt cataagttaa ctaaaagaaa atggcttata ttctataata cctacgtcaa
    10561 tcaacctgta cacaaatttt atttttgcat taacttaata ttgccgttaa ttatcttatc
    10621 tgcaatgttc gtttatctaa caatttcatt cccgcaatat ggacattatt ttatgttctt
    10681 attggcattg aattt
//
LOCUS       DAMQYT010000076        10543 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMQYT010000076 DAMQYT010000000
VERSION     DAMQYT010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10543
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.076"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            185..2170
                     /gene="uvrB"
                     /locus_tag="PGA33_001480"
     CDS             185..2170
                     /gene="uvrB"
                     /locus_tag="PGA33_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDG6473289.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            2178..5024
                     /gene="uvrA"
                     /locus_tag="PGA33_001481"
     CDS             2178..5024
                     /gene="uvrA"
                     /locus_tag="PGA33_001481"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDG6473290.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     HDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            5631..6563
                     /locus_tag="PGA33_001482"
     CDS             5631..6563
                     /locus_tag="PGA33_001482"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDG6473291.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            6569..7408
                     /locus_tag="PGA33_001483"
     CDS             6569..7408
                     /locus_tag="PGA33_001483"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDG6473292.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPSEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGSVSRAFLEQLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGR
                     FFIEGLRTDSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPTK
                     KVK"
     gene            7416..7901
                     /locus_tag="PGA33_001484"
     CDS             7416..7901
                     /locus_tag="PGA33_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDG6473293.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQLLNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            7909..9348
                     /locus_tag="PGA33_001485"
     CDS             7909..9348
                     /locus_tag="PGA33_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDG6473294.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDMEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYHNELFVSENDVMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            9415..10350
                     /gene="trxB"
                     /locus_tag="PGA33_001486"
     CDS             9415..10350
                     /gene="trxB"
                     /locus_tag="PGA33_001486"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDG6473295.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
BASE COUNT         3764 a         1426 c         2123 g         3230 t
ORIGIN      
        1 caagataaac gatttgattt aaggcaagca tagttagcac aactatgttt gtttttcttt
       61 gttcgacatt tttacgaaca aacgtttgct ttttgtgtga ctactttgct aaaatatgta
      121 atgagaaagc aaagagttga gcggaaatat aaataatacg ttgaaagagg aggcatatgt
      181 gacaatggtt gaacattatc cttttaaaat acattctgat tttgagcctc aaggtgacca
      241 accgcaagca attaaagaaa tcgtggaagg tattaaagcg gggaaaagac atcaaacttt
      301 attaggtgct actggcacag ggaaaacatt tacgatgagt aatgttatta aagaagttgg
      361 gaaaccaacg ttaattatcg cgcataacaa aacattagca ggacaattat atagtgagtt
      421 taaagaattt tttcctgaaa acagggtgga atactttgta agttactatg attattatca
      481 accagaggca tacgtaccgt ctactgacac ttttattgaa aaagatgcct caatcaatga
      541 tgaaattgat caactacgac attctgctac aagtgcatta tttgaacgcg atgatgtaat
      601 tattattgct agtgtaagtt gtatatatgg tttaggtaat cctgaagaat ataaagattt
      661 agtagtaagt gttcgagttg gtatggaaat ggatagaagt gaattactta gaaaacttgt
      721 agatgtgcaa tatacacgaa atgacatcga tttccaacga ggaacgtttc gagtgcgtgg
      781 tgatgtagtg gaaatattcc cagcctctaa agaagaactt tgtataaggg ttgagttttt
      841 cggcgatgag attgaccgta tccgagaagt taactaccta acaggtgaag tgttgaaaga
      901 aagagaacat tttgcgatat tcccagcttc tcacttcgta acacgtgaag aaaagttgaa
      961 agttgcgatt gaacgtattg aaaaagaatt ggaagaacga ttgaaagaat tacgagatga
     1021 gaataaatta ctagaagcgc aaaggttaga acagcgtacc aactatgatt tagaaatgat
     1081 gcgagagatg ggattctgtt caggaattga aaactattcc gtacatttaa ctttgcgacc
     1141 actgggttcg acaccatata ctttattgga ttactttggc gatgattggt tagtaatgat
     1201 tgatgaatca catgtgacat taccgcaagt tcgaggcatg tataacggag acagagcgcg
     1261 taaacaagtt ttggtggatc atgggtttag attaccgagt gcattagata accgtccact
     1321 taaatttgaa gaatttgaag aaaagacaaa acaacttgtg tatgtatctg caacgcctgg
     1381 accatacgaa attgaacata cggataagat ggttgaacaa attattcgtc ctactggttt
     1441 actggatcct aagattgagg ttagacctac tgaaaatcaa attgacgatt tattaagtga
     1501 aattcaaaca agagttgagc gtaatgaacg cgtacttgtt acaacgctca ctaaaaagat
     1561 gagtgaagat ttaaccacat acatgaaaga agcgggtatt aaagttaatt atctgcattc
     1621 agaaatcaag acattagaac gaattgaaat aattagagac ttacgaatgg gtacatatga
     1681 tgttatcgta ggtattaatt tattaagaga gggtattgat ataccagaag tttctctagt
     1741 tgtcatatta gatgcagata aagaagggtt tttacgttct aaccgctcat taattcaaac
     1801 aataggtaga gctgcgcgta acgataaagg tgaagtcatt atgtatgccg ataaaatgac
     1861 tgattcgatg aagtatgcaa ttgatgagac acaacgtcgt cgagaaatac agatgaaaca
     1921 taatgaaaaa catggtatta cacctaaaac aattaataaa aaaatacatg atttaattag
     1981 tgctactgtt gaaaatgacg aaaataatga caaagcacaa actgtgatac ctaagaagat
     2041 gacgaaaaaa gaacgtcaaa agacaatcga caatatagaa aaagaaatga aacaagcagc
     2101 gaaagattta gatttcgaga aagctacaga attaagagat atgttatttg aattaaaagc
     2161 agaagggtga caagtaaatg aaagaaccat ccatagtagt aaaaggtgct cgtgcgcata
     2221 acttgaaaga tattgatatc gaactaccta aaaataaatt aattgttatg acaggtttat
     2281 ctgggtcagg taaatcgtca ttagcattcg atactatata tgctgaagga caacgacgtt
     2341 atgttgaatc attaagtgcc tatgcgcgtc aatttttagg ccaaatggac aaaccagatg
     2401 ttgatacaat tgaaggatta tcgccagcaa tttcaataga tcaaaaaaca acaagtaaaa
     2461 atccaagatc aactgtagca acagtaacag aaatatatga ttatatacgt ttgttatatg
     2521 cacgtgttgg taaaccttac tgtccaaatc acaatataga aattgaatcg caaacagtac
     2581 aacaaatggt tgaccgcatt atggaattag aggcacgtac aaagattcaa ttattagcac
     2641 ctgtcatcgc tcatcgtaaa ggtagtcatg aaaagctaat cgaagatatt ggtaaaaaag
     2701 gttatgtacg tttaagaatc gatggcgaaa ttgttgatgt aaatgatgta cctactttag
     2761 ataagaacaa gaatcataca atagaagttg ttgtagaccg attagttgtt aaagatggaa
     2821 ttgaaacacg actagctgac tctatagaaa ctgccttaga gctttcagaa ggacaattaa
     2881 cagtcgatgt cattgacggg gaagacctta agttttcaga aagccatgct tgtcctatat
     2941 gtggattttc aatcggagag ttagaaccaa gaatgtttag ctttaacagt ccttttggtg
     3001 cttgtccgac atgtgatggc ttaggccaaa agttaacagt cgatgtagac ttggttgttc
     3061 ccgacaaaga taagacgcta aacgaaggtg caatagaacc ttggataccg acgagttctg
     3121 atttttatcc aacattgtta aaacgtgttt gtgaagttta taaaatcaat atggataaac
     3181 cttttaaaaa gttaacagaa cgtcaacgtg atattttatt gtatggttct ggtgacaaag
     3241 aaattgaatt tacatttaca caacgtcaag gtggtactag aaaacgaaca atggttttcg
     3301 agggtgtagt tcctaatata agtagacgat tccatgaatc tccttcagaa tatacacgtg
     3361 aaatgatgag taaatatatg actgaactac cttgcgaaac ttgtcatgga aagcgattga
     3421 gtcgtgaagc gttatctgtt tatgtaggtg gtttaaatat tggtgaagta gtcgaatatt
     3481 caatcagtca agcgctgaac tattataaaa acattgattt gtcagaacat gatcaagcga
     3541 ttgcaaatca aatattgaaa gaaattattt cccgactcac ttttttaaat aatgtgggac
     3601 ttgaatattt aacgttaaac agagcttcag gtacactttc aggtggtgaa gcacaacgta
     3661 ttcgattagc aacgcaaatt gggtcgcgtt tgactggtgt cttatatgta ttagatgagc
     3721 catcaattgg actgcatcaa agagataatg atcgattaat taatacactt aaagaaatga
     3781 gagatttagg aaatacttta attgtagttg aacacgatga tgatacaatg cgtgcggctg
     3841 attacttagt ggatataggt cctggtgctg gtgaacatgg agggcagatt gtgtctagtg
     3901 gtactcctca aaaggtaatg aaagataaaa aatcattaac aggacaatac ttgagtggta
     3961 agaaacgtat tgaagtacct gaatatcgca gaccggcttc agatcgtaaa atttctatac
     4021 gtggagctag aagcaacaat cttaaagggg ttgatgtgga cataccacta tcaatcatga
     4081 cggttgttac aggtgtatca ggttctggta aaagctcatt agtaaatgaa gtattataca
     4141 aatcattagc tcaaaaaatt aataaatcta aagtaaagcc aggattgtac gataagattg
     4201 aaggtattga tcaacttgat aaaattattg atattgatca atcaccaata ggtagaacgc
     4261 cacgctctaa tccagcaaca tatactggtg tgtttgatga tatacgtgat gtgtttgcgc
     4321 aaacaaatga agctaaaatt cgaggatatc aaaaagggcg ttttagtttt aatgtaaaag
     4381 gtggacgctg tgaagcttgt aaaggtgacg gtattattaa aattgaaatg cattttttac
     4441 ctgatgttta tgttccttgt gaagtgtgtg atggtaaacg atataatcgt gagacactag
     4501 aggttactta caaaggtaaa aatattgctg acattttaga aatgactgtt gaagaagcaa
     4561 cacaattttt tgaaaatatt cctaagatta agcgcaagtt acaaacacta gttgatgttg
     4621 gtcttggata cgtcacatta ggtcaacaag ctacaacgtt atcaggtggt gaggctcaac
     4681 gtgtgaaact tgcatctgaa cttcataaac gttcaactgg taaatctatt tatatcctag
     4741 atgaaccgac aacagggtta catgttgacg atattagtag attattaaaa gtattaaacc
     4801 gattagttga aaatggtgat actgttgtaa ttattgaaca taacctagat gttatcaaaa
     4861 cagcagacta tattatagac ttaggtcctg aaggtggtag tggcggtggt actattgttg
     4921 cgactggcac acccgaagat attgctcaga caaagtcatc atatacagga aagtatttaa
     4981 aagaagtact tgaacgagat aaacaaaata ctgaagataa ataagattaa aagaagtgaa
     5041 ggatgttata aatttatcct tcgcttcttt ttattaattt agtaatgaat agtagaaaga
     5101 aaagatgcgt aaaaagaatt atgttaagat agggtcaatc tagagtagtt aaacataaat
     5161 cgaactggga gtgggacaga aatgataaag aatcactaat gatttattat gtagtggttc
     5221 tttgtcatta gccacagcta ttgtgtactt aaaaatagga atgcatgagt gcaactcatg
     5281 cataagaaat actaatttct aaagaaaaag tatttcttta tgttggggcc ccgccaactt
     5341 gcattgtttg tagaatttct tttcgaaatt ctttatgttg gggccccgcc aactaattcc
     5401 aatatatcat tgtagagctt aggtcattga tttttggctc ggacttttat ggcgatatga
     5461 accatgtaaa ttaagcaagc aataaattaa tgattgatat tgacttgtaa aataataaca
     5521 ataatgaaca attaatattt attttagctt ttcaatgtag attggtgtta tatttttgat
     5581 atgataagaa gagatgtaag agtagggata aatacaattg aggtgaaccc atgttaacga
     5641 cagaaaaact agttgaaaca ttaaagttag atttaatcgc tggtgaagaa ggactatcga
     5701 agccaattaa aaatgctgat atatcaagac cgggcttaga gatggcaggt tatttttcac
     5761 attatgcgtc agatagaata caactattag gaacaacgga actatcgttt tacaatttat
     5821 taccagataa ggatcgcgca ggtcgtatgc gtaaactatg cagaccagaa acgcctgcaa
     5881 ttattgtgac acgtggattg cagccaccag aagaattagt tgaagctgca aaagaattaa
     5941 ataccccact tatagttgct aaagatgcga ctacaagttt aatgagtcgc ttaacaacgt
     6001 ttttagagca tgcacttgca aagacgacat ctttacatgg tgttttagta gatgtttacg
     6061 gtgttggtgt actaattacc ggtgattcag gaataggtaa aagtgagact gcgttggaat
     6121 tagttaaacg tgggcataga ttagtagcag atgataatgt agaaatacgt caaattaata
     6181 aagatgaact aatagggaaa ccaccaaagt taatagaaca tctattagaa atacgtggac
     6241 taggtattat caatgttatg actttatttg gcgcgggttc aatattaact gaaaaacgaa
     6301 ttagattaaa tattaatttg gaaaactgga acaagcaaaa gttatatgac cgcgtaggtc
     6361 ttaatgaaga gacgctaagt attttagata ctgaaatcac taaaaaaaca atacctgtaa
     6421 gacctggtag aaatgttgcg gtaattattg aggtcgctgc aatgaactat cgattaaata
     6481 tcatgggcat taacacggcc gaagaattta gtgaaagatt aaatgaagaa attatcaaga
     6541 acagtcataa gagtgaggag taggttgaat gggtattgta tttaactata tagatcctgt
     6601 ggcatttaac ttaggaccac tgagtgtacg atggtatgga attatcattg ctgtcggaat
     6661 attacttggt tactttgttg cacaacgtgc actagttaaa gcaggattac ataaagatac
     6721 tttagtagat attatttttt atagtgcact atttggattt atcgcggcac gaatctattt
     6781 tgtgattttc caatggccat attacgcgga aaatccaagt gaaattatta aaatatggca
     6841 tggtggaata gcaatacatg gtggtttaat aggtggcttt attgctggtg ttattgtatg
     6901 taaagtgaaa aatttaaacc catttcaaat tggtgatatc gttgcgccaa gtataatttt
     6961 agcgcaagga attggacgct ggggtaactt tatgaatcac gaggcacatg gtggatcggt
     7021 gtcacgcgct tttttagaac aattacattt gcctaatttt ataatagaaa atatgtatat
     7081 taacggccaa tattatcatc caacattctt atatgaatcc atttgggatg tcgctggatt
     7141 tattatctta gttaatattc gtaaacattt aaaattagga gaaacattct ttttatattt
     7201 aacttggtat tcaattggtc gattctttat agaaggatta cgtacagata gcttaatgct
     7261 cacaagtaat attagagttg cacaattagt atcaattctt ttaattttaa taagtataag
     7321 tttaattgta tatagaagga ttaagtataa tccaccgttg tatagcaaag ttggggcgct
     7381 tccatggcca acaaaaaaag tgaagtagtg atagtttgag gaaattttta tcaaaaacac
     7441 atcatcatac aaacccttta tggcgtgtat accgtcttgt taaattttcg aaagttttta
     7501 agaatgtaat tatcattgaa ttttcgaaat ttattccaag tatggtactg aaaagacata
     7561 tatataaaca acttttaaat attaatatcg gtaatcaatc gtcgatagct tataaagtaa
     7621 tgttagatat tttttaccca gaactgatta cgattggtag taacagtgtt attggttaca
     7681 atgtaacaat tttgacgcat gaagcattag ttgatgaatt tcgttatgga ccagtgacga
     7741 taggatctaa cactttgatt ggtgcaaatg ctaccatttt acccggtata acgattggtg
     7801 acaatgtaaa agttgcagct ggtacggttg tttcaaaaga tataccggat aatggatttg
     7861 catatggcaa ccctatgtat ataaaaatga ttaggaggtg acaattttat ggcgcaaaag
     7921 aataataatg taattccaat gacttttgat gatgcatttt atcgtaaaat ggctaaacag
     7981 aagtttaaac aaagagaata taaacgagct gctgaatact ttgaaaaagt gttagaattg
     8041 tcacctgatg atatggaaat tcaaattgat tatgcacaat gtctagtgca acttggtatt
     8101 gctaaaaaag cagaacattt attttatgac aatattattt ataataggca tctagaagat
     8161 agcttttatg aattgagtca gctcaacatt gaagttaacg aaccaaacaa ggcattcttg
     8221 tttggtatta attatgttat tgttagcgac gaccaagatt atagagatga attagatcaa
     8281 atgtttgatg tgaaatatca aagtgaagaa caaattgaac ttgaagctca attgtttgta
     8341 gttcaaatac tattccaata tcttttttct caaggtcgat taaaagatgc aaagaattat
     8401 gtcttacatc aaccacaaga agttcaagat catcgtgtag tacgtaattt attggcaatg
     8461 tgttatttat atctcggtga atatgatacg gctaaagcat tgtacgaagc actattacaa
     8521 gaggatagta cagatatata tgcattatgc cattatactt tgctacttta taacactaag
     8581 gaaaatgaac aatatcaaaa atatttaaaa atattaaaca aagttgtacc tatgaatgac
     8641 gatgaaagtt ttaaattagg tattgtatta agttatttaa agcagtatcg tgcatcacaa
     8701 caattgttgt accctttata taaaaaaggg aaatttttat caattcaaat gtacaatgct
     8761 ttagcatata attattatta tttaggtgaa gaagacgaaa gtcattacta ctgggataaa
     8821 ttgaagcaaa tttctaaagt ggaaattgga catgcgcctt gggtaattga aaatagcaaa
     8881 gaagtttttg accaacatat tttgccatta cttcaaagtg atgacagtca ttatcgttta
     8941 tatggtattt ttttattgga tcaattaaat ggtaaagaaa ttgtgatgac ggaaagtatt
     9001 tggcaggttt tggaaaatct aaataattat gagaaattgt atttaacgta tttagttcaa
     9061 ggtttaacgc tcaataaatt agacttcatt catcgcggct tattaacgct ttaccataat
     9121 gaattatttg taagtgaaaa tgatgtaatg gttgcatgga ttaatcaagg tgaactcata
     9181 attgctgaaa aagtagattt aactgatgtt gagccatata tcggtgcgtt tatttatttg
     9241 tattttaaaa atcaacctcg aaacgttaca aagaagcaaa ttacaacatg gttaggcata
     9301 acacaatata aactgaacaa aatgattgaa tttctcttga gcatatagat ttatgaaaag
     9361 ttagatttat tatataatgc gcataatgat taataatgag gaggcgttaa taaaatgact
     9421 gaaatagatt ttgatatagc aattatcggt gcaggtccag ctggtatgac tgctgcagta
     9481 tacgcatcac gtgctaattt aaaaacagtt atgattgaaa gaggtattcc aggcggtcaa
     9541 atggctaata cagaagaagt agagaacttc cctggtttcg aaatgattac aggtccagat
     9601 ttatctacaa aaatgtttga acacgctaaa aagtttggtg cagtttatca atatggagat
     9661 attaaatctg tagaagataa aggcgaatat aaagtgatta actttggtaa taaagaatta
     9721 acagcgaaag cggttattat tgctacaggt gcagaataca agaaaattgg tgttccgggt
     9781 gaacaagaac ttggtggacg cggtgtaagt tattgtgcag tatgtgatgg tgcattcttt
     9841 aaaaataaac gcctattcgt tatcggtggt ggtgattcag cagtagaaga gggaacattc
     9901 ttaactaaat ttgctgacaa agtaacaatc gttcaccgtc gtgatgagtt acgtgcacag
     9961 cgtattttac aagatagagc attcaaaaat gataaaatcg actttatttg gagtcatact
    10021 ttgaaatcaa ttaatgaaaa agacggcaaa gtgggttctg tgacattaac gtctacaaaa
    10081 gatggttcag aagaaacaca cgaggctgat ggtgtattca tctatattgg tatgaaacca
    10141 ttaacagcgc catttaaaga cttaggtatt acaaatgatg ttggttatat tgtaacaaaa
    10201 gatgatatga caacatcagt accaggtatt tttgcagcag gagatgttcg cgacaaaggt
    10261 ttacgccaaa ttgtcactgc tactggcgat ggtagtattg cagcgcaaag tgcagcggaa
    10321 tatattgaac atttaaacga tcaagcttaa ttcgaagtcg aattaagatg ttgagctgta
    10381 aattatttgg atatttattt taatagtgtc atcacagcgt taaaataatg tcttactttt
    10441 aaattaaagc aaattatata gaaaactaga acttagtacg tatcatttgt gcgtttcaat
    10501 gagttctagt ttttttatat gttatattaa acttataact tta
//
LOCUS       DAMQYT010000077        10504 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMQYT010000077 DAMQYT010000000
VERSION     DAMQYT010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10504
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.077"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            11..1087
                     /locus_tag="PGA33_001487"
     CDS             11..1087
                     /locus_tag="PGA33_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HDG6473296.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
     gene            1107..1901
                     /locus_tag="PGA33_001488"
     CDS             1107..1901
                     /locus_tag="PGA33_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDG6473297.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNFFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            1973..2080
                     /locus_tag="PGA33_001489"
     CDS             1973..2080
                     /locus_tag="PGA33_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473298.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(2091..3653)
                     /locus_tag="PGA33_001490"
     CDS             complement(2091..3653)
                     /locus_tag="PGA33_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDG6473299.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGYEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(3858..4955)
                     /locus_tag="PGA33_001491"
     CDS             complement(3858..4955)
                     /locus_tag="PGA33_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HDG6473300.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGRRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVNGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVIIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            5327..5887
                     /locus_tag="PGA33_001492"
     CDS             5327..5887
                     /locus_tag="PGA33_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDG6473301.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            5940..6869
                     /locus_tag="PGA33_001493"
     CDS             5940..6869
                     /locus_tag="PGA33_001493"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDG6473302.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(7064..7237)
                     /locus_tag="PGA33_001494"
     CDS             complement(7064..7237)
                     /locus_tag="PGA33_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473303.1"
                     /translation="MNVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            7289..8668
                     /locus_tag="PGA33_001495"
     CDS             7289..8668
                     /locus_tag="PGA33_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDG6473304.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKIARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(8788..9816)
                     /locus_tag="PGA33_001496"
     CDS             complement(8788..9816)
                     /locus_tag="PGA33_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDG6473305.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            10086..10487
                     /locus_tag="PGA33_001497"
     CDS             10086..10487
                     /locus_tag="PGA33_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HDG6473306.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
BASE COUNT         3552 a         1824 c         1596 g         3532 t
ORIGIN      
        1 ggtgttgtta atgttattta aagaggctca agctttcata gaaaacatgt ataaagagtg
       61 tcattatgaa acgcaaatta tcaataaacg tttacatgac attgaactag aaataaaaga
      121 aactgggaca tatacacata cagaagaaga acttatttat ggtgctaaaa tggcttggcg
      181 taattcaaat cgttgcattg gtcgtttatt ttgggattcg ttaaatgtca ttgatgcaag
      241 agatgttact gacgaagcat cgttcttatc atcaattact tatcatatta cacaggctac
      301 aaatgaaggt aaattaaagc cgtatattac tatatatgct ccaaaggatg gacctaaaat
      361 tttcaacaat caattaattc gctatgctgg ctatgacaat tgtggtgatc ctgctgaaaa
      421 agaagttaca cgcttagcaa atcacttagg ttggaaagga aaaggtacta attttgacgt
      481 gttaccactg atttaccaat tacctaatga gtcagttaaa ttttacgaat atcctacttc
      541 attaattaaa gaagtaccta ttgaacataa tcattatcca aaattaagaa aattgaactt
      601 aaaatggtat gcagtcccta tcatttccaa tatggactta aaaatcggtg gcattgtata
      661 tccaactgca ccctttaacg gttggtatat ggtaactgaa attggcgtac gtaactttat
      721 tgatgattac cgttacaatt tactagaaaa agttgcagat gcgtttgaat ttgatacact
      781 taaaaataat tcatttaata aagatcgagc acttgttgaa ttgaactatg ctgtgtatca
      841 ttcctttaaa aaagaaggcg tatcaattgt cgatcatttg accgctgcaa agcaattcga
      901 actattcgaa cgtaacgaag cacaacaagg tcgtcaagtt accggaaaat ggtcttggct
      961 agcaccgcca ttatctccaa cattgacgtc aaattatcat cacggatatg acaatacagt
     1021 aaaagatcca aactttttct ataaaaagaa agaatcaaat gctaaccagt gccctttcca
     1081 tcattaagtt agtagaggtg tagcatatgc aattatatta tttaggccct aaaggtacat
     1141 tttcatattt ggcttgtcgt cagtattttt ctgaaaatga agcaactttt caaccaaagt
     1201 caaatttttt tgaagtcatc aaggcagttg cagatgatga cacatcgatt ggtgttgtcc
     1261 ctattgaaaa ttctatagaa ggaacaatca acattgtggc agatgcactt gcacagcaag
     1321 atgtctttgc acacggtgaa atacgtttag atattaactt tgcattatat ggtaatggaa
     1381 cagattccat ttctgatatt aaaaaagtgt attctattgc accagcaatc agtcaaacaa
     1441 caaattacat acatcaacat caatttgact atgattatgt cgacagtaca attcaaagtt
     1501 tgactaaaat tgaaaatggc gttgcagcaa ttgcaccttt aggtagtggc gaagcttatg
     1561 gttttacacc tattgataca catattgaag actatccaca taacgtgaca cgttttttag
     1621 ttataaaaaa tcagcagcaa tttgatcaaa atgcgacatc attgatgttt ttaattacac
     1681 caatgcatga taaacctggg ttgttagcaa gtgtgcttaa tacatttgct ttgtttaata
     1741 ttaatttatc ctggattgag tctcgtcctt taaaaacaca attaggtatg tatcgatttt
     1801 tcgttcaggc tgattctgca attacaaccg atataaagaa agtcattgct attttagaaa
     1861 ccttagattt caaagtagaa atgattggtg cttttaatta aactttattt tctaggttct
     1921 acaatttaaa taaaataaag agtcaaccaa ggattaaaat aggaatccat ccttggttga
     1981 ctttcttttg tttgtattgt ttttacacga tgctgcggta tacgtttcgt taccactcgt
     2041 ccaagcaatt gctaatgtaa ccttttattt ttcgttctaa tctgcaatta ctatttcaat
     2101 ggcagtggtt gatttatatc aatacctaaa actataggca ttacataata cacgactaaa
     2161 ataataatta ttgcactgat taagtttacc cagaatccta ctgatgccat ttgtttgata
     2221 gatattttac cagaaccaaa gataattgca ttcggtggtg tccctactgg taacatgtat
     2281 gcacagttag ccgccatagc tgcaggtgcc ataagtagta atggatgcac tccaacagca
     2341 acagacaacg ttgctaaaat cggtaaaatc atcgttgcag ttgcagtatt agatgtcact
     2401 tcagttaaaa ataagacaaa gattgttatg acaattacaa taagaatcgg actaacacca
     2461 tttaatgatt tcaactgttc gcctaaccat tttgctaaac cactttcaga aatacctttc
     2521 gctaatgcta aaccgccacc aaataaaatt aatacacccc aagggagctc ttttgcaact
     2581 tcccagtcaa tgatacggcg atgtttttca gtatttttag ctggaataat aaataataat
     2641 attgatataa aaatagcaat cgtaccatct gcaacagatg acgtaacttc ccattttttc
     2701 agaagaaact ctcttgtaat ccataataag ctagcaagta caaagatagt ttgtactacc
     2761 ttttcttcat acttcatttt gccaagctcg tctaactttt gtttaattaa cgtctgacca
     2821 ccaggtaaat atttcaaatc atgtctaaac gcaacatatc ttaaatagag ccaagtaata
     2881 cctaacaaaa caatgaccgt tggaatccct acaatcatcc atttagcaaa actaatttca
     2941 tatccaaaat gttgcatgta ttgtcctttt aaaataatta atggcggggt tccgattaat
     3001 gtacccaagc caccaatcgt acctgcatag ccaattgcta aaactagaga tttttcaaac
     3061 ttttgaatac ttgtttgatt cgtattggct tcttgtaaat catgtgcttc cttaataatt
     3121 gctaaaccaa tcggaatcat aatcattaca gctgcagtgt tcgatacaaa catagataag
     3181 aatcctgttg ccaccatgaa tccaagtaat atttttgaag tgcttgcacc aatcaaatta
     3241 ataattgtca acgctactct cgtatgtaaa ttccatcttt ccattgcaat tgccaaaata
     3301 aatccaccta aaaacaaaaa gataatatca ttgccatatt cggatgatac ttgttctggt
     3361 gtaagtatat gacctaatgg taataacaca attggtaata agctcgttgc tgcaatagga
     3421 attgcttcag taatccacca agtcgcaatc cataaagtaa tcgctaaaac atagacgcct
     3481 ttccaaggta agtcttgtgg atgaaagaat aataatgtta ataggaaaag taatggacct
     3541 aatattagtc caataagttg tcccgcacta aacggtttct ttttctttga ttttgaaaag
     3601 aatgttaaat accttttcga tatcattgat tgatgttgat tgaaataagc cataaatttc
     3661 cccctcatga tgattatgac aagctacttc aactcactat attagagtat agaaagttgt
     3721 ctatcttgta ttatcattac cgtcaaaata ccccttttat cagataattg ggtttattat
     3781 agcatattta taccgtttta tgaatgcgct ttcaaaaatt atctactatt cttaatccta
     3841 atgtcatgcg aaacttttta cttttttatt tcatctacat tgattgtagt aacttgaata
     3901 cccgcttcaa caggacttaa aaacactgta tcaatatctt ctaaatgaat tgattgagtc
     3961 gaattattga atgtattccc aacgattaat acatctttat gtttaacatt attataatta
     4021 cgtacatgta tcgcatcttt agacattgat cctttaaatt catttccaat tatattcata
     4081 tttatgcctg cttgggaacc taagtctttt cctgtcatca catctgctgc atttttacca
     4141 tctctaactc ctaaatatct aatgccaccc tcacagttaa taaacttatt attaataatg
     4201 aaagcatctt tatacttcaa gggagttaat gcataacctt gtatatcttc aaatatatta
     4261 tttctaatat gaatattctc atagtatcga ttatgtctac ttgcatgtga gccaatagca
     4321 cgattccaac ttcccatttc gggcaattct gaaggtccaa aataacaatc ttcgataatg
     4381 acatttttcg ttatcgtacc atctgtcgtt ccgaattttg gaaaagcacc aggtacttga
     4441 atgtctaatt gtattgcttc agaataaaag cgttcgccac tatagtctat gaatccttca
     4501 aatgaacagc ttttaatata gagtccgtta atcccacaag catcaattgc atgaccattc
     4561 actacattct taatggtcac acctattaat tgaatatctt cagcatgccc aatgcacata
     4621 gctgtattgt tataaggata cgatacacca ttcatatcaa acttgccgcc tttaatatga
     4681 atatgactgt gtccattata accacgataa aaaccaaagc gacgaccatt ttttaataaa
     4741 ggaccagaat gacatcggcg taaaatagtt tcattatcta acaaaagtgt tgtattgcca
     4801 tatatcgtta atggtttgca aatatcatac gtccctttcg gtatataaac tgtcgttggt
     4861 ttacatcttc cacgatttaa cgcacgttgt attgctcgag tatctttagt tttgtttatg
     4921 cctgttaacc cataatcttt catattgata tacataaatc gtcatccttt gtctattttc
     4981 taattactca tatattaacc aattattcaa attataatcg tgttaggttg atttaacaga
     5041 tagtcataaa tctcgtgatg ccatctatta gcatttttat attcaaaaca tcaacacttg
     5101 atattagttc taagtttgtc acatttagta ttctattaca gctattttaa aactatcgca
     5161 aaaacttctt atattatttg ttgattgcgt gatttgctct tccaaaaaag ttgattaatc
     5221 tatatactta atctatattt atatattaac cattagggtt aaaaattact ctagcattta
     5281 tgaatagatg ggagtttatt ttattattat ataggagaga tgttgaatga cacatcgcgc
     5341 actattagtt gttgactatt catatgactt tatcgcagac gacggcttac taacatgcgg
     5401 taaacctgga caaaatattg aagattttat tgtttctcgt atcaatgact ttaattatta
     5461 tcaagaccat atattctttt taatggattt acattattta catgacattc atcatcctga
     5521 aagtaaatta ttcccaccac acaatatcgt agatacaagt ggtagagaat tatacggtaa
     5581 agtaggtaaa ttatacgaaa caattaaagc gcaacctaat gtacatttca ttgataaaac
     5641 gcgctatgat tcgttctttg gtaccccgct tgatagttta ttgagagaaa gaagtattaa
     5701 tcaagtcgaa atcgttggtg tatgtaccga tatttgcgtg ttacatacag caatttctgc
     5761 atacaactta ggttataaaa tttcagtacc tgctgaggga gtggcttcat ttaatcaaaa
     5821 agggcatgaa tgggcacttg cacatttcaa aaactcatta ggtgcagagg tagaacaaca
     5881 cgtttaaatc gtgctaaaat aattataaag aatacaattt acaagggaga tatttgacaa
     5941 tggctaaaac atatattttc ggacataaga atccagacac tgatgcaatt tcatctgcga
     6001 ttattatggc agaatttgaa caacttcgag gtaattcagg agccaaagca taccgtttag
     6061 gtgatgtgag tgcagaaact caattcgcgt tagatacatt taatgtacct gctccggaat
     6121 tattaacaga tgatttagat ggtcaagatg ttatcttagt tgatcataac gaattccaac
     6181 aaagttctga tacgattgcc tctgctacaa ttaagcatgt aattgatcat cacagaattg
     6241 caaatttcga aactgctggt cctttatgtt atcgtgctga accagttggt tgtacagcta
     6301 caattttata caaaatgttt agagaacgtg gctttgaaat taaacctgaa attgccggtt
     6361 taatgttatc agcaattatc tcagatagct tacttttcaa atcaccaaca tgtacacaac
     6421 aagatgttaa agcagctgaa gaattaaaag atattgctaa agttgatatt caaaagtacg
     6481 gcttagatat gttaaaagca ggtgcttcaa caactgataa atcagttgaa ttcttattaa
     6541 acatggatgc taaatcattt actatgggtg actatgtgac tcgtattgca caagttaatg
     6601 ctgttgacct tgacgaagtg ttaaatcgta aagaagattt agaaaaagaa atgttagctg
     6661 taagtgcaca agaaaaatat gacttatttg tacttgttgt tactgacatc attaatagtg
     6721 attctaaaat tttagttgta ggtgctgaaa aagataaagt tggcgaagca ttcaatgttc
     6781 aattagaaga tgacatggcc ttcttatctg gtgtcgtttc tcgaaaaaaa caaatcgtac
     6841 ctcaaatcac tgaagcatta acaaaataat actatattac tgtctaatta tagacatgtt
     6901 gtatttaact aacagttcat taaagtagaa tttatttcac tttcaatgaa ctgtttttta
     6961 tttacgtttg actaatttac aacccttttt caatagtagt ttttattcct ttagctaccc
     7021 taacccacag attagtgatt tctatacaat tccccttttg tctttacatt ttcttaaaat
     7081 atttgcgatg ttgagtataa atttttgttt tcttcctacc tttttcgtta tgattaaagt
     7141 tataaatatt attatgtaca cgattcatcg ctctattttc aactttcaac atatataatt
     7201 cgaaagacca tttaaaatta acggccacaa cattcaaatc aattaatcgc tttttccaaa
     7261 ataatcatat aaggaggttc ttttcattat gaatatcatt gagcaaaaat tttatgacag
     7321 taaagctttt ttcaatacac aacaaactaa agatattagt tttagaaaag agcaattaaa
     7381 gaagttaagc aaagctatta aatcatacga gagcgatatt ttagaagcac tatatacaga
     7441 tttaggaaaa aataaagtcg aagcttatgc tactgaaatt ggcataactt tgaaaagtat
     7501 caaaattgcc cgtaaggaac ttaaaaactg gactaaaaca aaaaatgtag acacaccttt
     7561 atatttattt ccaacaaaaa gctatatcaa aaaagaacct tatggaacag ttttgatcat
     7621 tgcaccattt aactatcctt ttcaactagt gttcgaacct ttaatcggtg ctattgcagc
     7681 aggtaatacg gcaattatta aaccatctga gttgacgcct aatgttgcac gagtgattaa
     7741 acggttaatc aatgaaacat ttgatgcaaa ttacattgaa gttattgagg gaggaattga
     7801 agaaacgcaa acgttaattc acttaccttt tgactatgtc ttctttacag gaagtgaaaa
     7861 tgtaggcaaa atcgtttatc aagctgccag cgaaaattta gttcctgtga cattagaaat
     7921 gggcggtaaa tctccagtca tcgttgatga aacagcgaat attaaagttg ctagtgagcg
     7981 catttgtttt gggaaattca ctaatgccgg ccaaacatgt gttgcaccag attacatttt
     8041 agtacacgaa tctgtaaaag atgatttaat cacagcccta tcaaaaacgt tgcgtgaatt
     8101 ttatggtcaa aatatacaac aaagtccaga ttatggccgc attgtaaacc ttaaacatta
     8161 tcatcgtctg acttcattac ttaacagtgc acaaatgaat attgtatttg gtggtcatag
     8221 tgatgaggat gaacgttata tagaaccaac attgttagat cacgttacaa gtgattcagc
     8281 aattatgcaa gaagaaattt ttggtcctat cttaccgatt ttaacgtatc agtcattgga
     8341 tgaagcaata gcctttattc accaaagacc aaaacctttg agtttatatt tatttagcga
     8401 agatgaaaat gctacacaac gtgtaataaa cgagctatca tttggcggcg gcgctattaa
     8461 tgatacattg atgcacctag cgaatcctaa attaccattt ggtggtgttg gcgcctcagg
     8521 tatgggacgc tatcatggta aatattcatt cgacactttt acacatgaaa aaagctacat
     8581 tttcaaatct acacgattag aatcaggtgt ccatttacca ccatataaag gtaaatttaa
     8641 atacatcaaa gctttcttta aaaattaatt tttgagacaa aaataaacca tgcttccata
     8701 atttgatgtt cttgaaaaca ataaatttca aggtatacac aaattttttg gaaacatggt
     8761 tttcttttta tcatgattaa ttaaagatta aaatatgaca catacacctt cagatgctac
     8821 acgagtgtta tcacatagcg taattttacc tgtttcttta tttctttcga aaacagttac
     8881 aactgaatct ccttgctcat gagcacaaac taaaaggtca tctgatgagg caatattaaa
     8941 atctcttggg aattgaccac cactttcagt aattgttact agttctaagt gttgaccatt
     9001 atcaagaact ttaaaaattg caatgctatc atgccctcta ttagatacat ataagaattg
     9061 ttgatcatga gataaacgca ctgctgcaag tttagtatct ccatcaaagt tttcaggaat
     9121 tgttaaatga cgctcgagct cttcaaattt accgtcatta tattctgcaa cactcacagt
     9181 atttgataat tcgtgtacga cataagcaaa ttttccatta tcatgaaatt caatatgtct
     9241 tgtcccatca ctatctttaa ataaagattc tttataaaat tcaaacccgt tgtcatcaaa
     9301 tttataagta acgatacgat cagcacctaa atctgttact gcaacgtact taccatctgg
     9361 agtttgatta atataatgtg cgtgtggatg atcttgtctt tcatgtgtac ctgttggaaa
     9421 atcatgtgca agttcttgaa ttagacgtat aatttcacct gtgtgcgtat ttaattcata
     9481 catacgtatg atgccagcac catataccgc ttcaaataaa tatcgtttat cttctgaaat
     9541 cgatacataa caacctgtac cagcttttga agacaaacat ttatttaata aatgcaattc
     9601 accattgtca tcaattttca aacttgcaac accgcattgt tctccttctt tgttgattcc
     9661 atataaaact tcattattac gcaccaaata tgtagacgct tctaattcaa atcctgtttc
     9721 taataaatca atacgtgact gattttcgtt taattcaaaa cgataaatcc ctttaccatt
     9781 ctttttagtg taagaaccaa tatatccatt tgtcattact tttacccctc catgttagtc
     9841 ttattttcat tttattaaat tttagctaga attaacaatc atttatcatt tccttaaagc
     9901 taaaactatt taactacttt aaaagaacta tttgctgtga tatgtcatta ttcgatttta
     9961 attaatccct cttttttact tacacgagca ttaattatta acgcgtttat gcttccacaa
    10021 aatttttgtt gcaaaataga acacatgttc gtataatgta attatcagat tggggtgaat
    10081 taaggatgac aagttatgaa gaagaaactg attatcgtaa cgttccaaga gaattactag
    10141 ataacaatat acctatggga agaggcatga ttaaatgggc tccttttgca acattacccg
    10201 aacaattcga aacgattcaa caatacatta tcgaccaaaa caaaattaca cgtcctgtat
    10261 tatcagatga tcaattggct gagctaaaca ttcggctaca cgaggcgcta caatatgccc
    10321 aacctgtaga agttaaattt tataataatg gctttgtaga ttcagtacgc ttaaccattt
    10381 atcgtattga tgctattaat tatgaaattg atggttatgt ttacaatcag caacaacgtc
    10441 aaaaaatttc tatctttgat atcttagata ttgtcttgtt accttaatgc tttgaacata
    10501 atta
//
LOCUS       DAMQYT010000078        10449 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMQYT010000078 DAMQYT010000000
VERSION     DAMQYT010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10449
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.078"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            144..1187
                     /gene="recA"
                     /locus_tag="PGA33_001498"
     CDS             144..1187
                     /gene="recA"
                     /locus_tag="PGA33_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDG6473307.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
     gene            1541..3100
                     /gene="rny"
                     /locus_tag="PGA33_001499"
     CDS             1541..3100
                     /gene="rny"
                     /locus_tag="PGA33_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDG6473308.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
     gene            complement(3397..3612)
                     /locus_tag="PGA33_001500"
     CDS             complement(3397..3612)
                     /locus_tag="PGA33_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473309.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
     gene            3786..4583
                     /locus_tag="PGA33_001501"
     CDS             3786..4583
                     /locus_tag="PGA33_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDG6473310.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            4723..6483
                     /locus_tag="PGA33_001502"
     CDS             4723..6483
                     /locus_tag="PGA33_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HDG6473311.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIYGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSDGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            6484..7350
                     /locus_tag="PGA33_001503"
     CDS             6484..7350
                     /locus_tag="PGA33_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HDG6473312.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            7444..7737
                     /locus_tag="PGA33_001504"
     CDS             7444..7737
                     /locus_tag="PGA33_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HDG6473313.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            7871..9415
                     /gene="miaB"
                     /locus_tag="PGA33_001505"
     CDS             7871..9415
                     /gene="miaB"
                     /locus_tag="PGA33_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDG6473314.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFIKEVE
                     PEMVIQ"
     gene            9416..9781
                     /locus_tag="PGA33_001506"
     CDS             9416..9781
                     /locus_tag="PGA33_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HDG6473315.1"
                     /translation="MYNKDDVLNQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            9808..10299
                     /gene="thiW"
                     /locus_tag="PGA33_001507"
     CDS             9808..10299
                     /gene="thiW"
                     /locus_tag="PGA33_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HDG6473316.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
BASE COUNT         3843 a         1455 c         2001 g         3150 t
ORIGIN      
        1 aagcacgttt gttcgttttt gcgttttgat ttcaagattt tatacgaaca aatattcgca
       61 aaacacttgt attttatttt gaatccttgt atagtattgg taagataatt aaaagatagc
      121 aatttcaatt aggaggtctc gctttggata acgatcgtca aaaagcttta gatacagtaa
      181 ttaaaaatat ggagaaatct ttcggtaaag gtgccgtaat gaagttgggt gacaatatag
      241 gtcgccgagt ttcaactaca tcaactggtt cagttacatt agataatgcg ctaggtgtag
      301 gtggctatcc taaaggacga attattgaaa tttatggtcc tgaaagttct ggtaagacaa
      361 cagtagcgct tcacgctatt gctgaagtac aaagtaatgg cggggtggca gcatttatcg
      421 atgctgaaca tgctttagat ccagaatatg ctcaagcatt aggcgtagat atcgataatt
      481 tatatttatc gcaaccggat catggtgaac aaggtcttga aatcgccgaa gcatttgtta
      541 gaagtggtgc agttgatatt gtagttgtag actcagttgc tgctttaaca cctaaagctg
      601 aaattgaagg agaaatggga gacactcacg ttggtttaca agctcgttta atgtcacaag
      661 cgttacgtaa actttcaggt gctatttcta aatcaaatac aactgctatt ttcatcaacc
      721 aaattcgtga aaaagttggt gttatgttcg gtaatccaga gactacacca ggtggacgtg
      781 cattaaaatt ctatagttca gtaagactag aagtacgtcg tgcagaacag cttaaacaag
      841 gacaagaaat tgtaggtaat agaactaaaa ttaaagtcgt taaaaataaa gtggcaccac
      901 catttagagt agctgaagtt gatattatgt atggacaagg tatttctaaa gagggtgaac
      961 ttattgattt aggtgttgaa aacgacatcg ttgataaatc aggagcatgg tattcttaca
     1021 atggcgaacg aatgggtcaa ggtaaggaaa atgttaaaat gtacttgaaa gaaaatccac
     1081 aaattaaaga agaaattgat cgtaaattga gagaaaaatt aggtatatct gatggtgatg
     1141 ttgaagaaac agaagatgca ccaaagtcat tatttgacga agaatagtac acaaatttat
     1201 atctatagtt aaacttagca aatatcctta taggattgat tgaaagtgat attcatctca
     1261 taaagctaga ataatatcta actttatggg atacactaca aatcgagact ataaggtttt
     1321 ttattttatt tattattaca ttatcaatag ttttataatc gagcttcaaa actttagaaa
     1381 atagtagaaa tagcattcaa tatagtgcaa aagtgcaaat tgataacttg acacttatct
     1441 cctataaacc gtacaattaa tttgtatgat ttatatataa tttcataaag tcatattgaa
     1501 tttcatataa agagcaaacc ctagaaaagg aggtgtttgt gtgaatttat taagcctcct
     1561 actcattttg ctggggatca ttctaggagt tgttggaggg tatgttgttg cccgaaattt
     1621 gttgcttcaa aagcaatcac aagctagaca aactgccgaa gatattgtaa atcaagcaca
     1681 taaagaagct gacaatatca aaaaagagaa attacttgag gcaaaagaag aaaaccaaat
     1741 cctaagagaa caaactgaag cagaactacg agaaagacgt agcgaacttc aaagacaaga
     1801 aacccgactt cttcaaaaag aagaaaactt agagcgcaaa tctgatctat tagataaaaa
     1861 agatgagatt ttagagcaaa aagaatcaaa aattgaagaa aaacaacaac aagtagatgc
     1921 aaaagagagt agtgttcaaa cgttaataat gaagcatgaa caagaattag aacgcatctc
     1981 cggtctcact caagaagaag ctattaatga gcaacttcaa agagtagagg aagaactgtc
     2041 acaagatatt gcagtacttg ttaaagaaaa agaaaaagaa gctaaagaaa aagttgataa
     2101 aacagcaaaa gaattattag ctacagcagt acaaagatta gcagcagatc acacaagtga
     2161 atcaacggta tcagtagtta acttacctaa tgatgagatg aaaggtcgaa tcattggacg
     2221 agaaggacga aacatccgca cacttgaaac tttaactggc attgatttaa ttattgatga
     2281 cacaccagaa gcggttatat tatctggttt tgatccaata agaagagaaa ttgctagaac
     2341 agcacttgtt aacttagtat ctgatggacg tattcatcca ggtagaattg aagatatggt
     2401 cgaaaaagct agaaaagaag tagacgatat tattagagaa gcaggtgaac aagctacatt
     2461 tgaagtgaac gcacataata tgcatcctga cttagtaaaa attgtagggc gtttaaacta
     2521 tcgtacgagt tacggtcaaa atgtacttaa acattcaatt gaagttgcgc atcttgctag
     2581 tatgttagct gctgagctag gcgaagatga gacattagcg aaacgagctg gacttttaca
     2641 tgatgttggt aaagcaattg atcatgaagt agaaggtagt catgttgaaa tcggtgtaga
     2701 attagcgaaa aaatatggtg aaaatgaaac agttattaat gcaatccatt ctcatcatgg
     2761 tgatgttgaa cctacatcta ttatatctat ccttgttgct gctgcagatg cattgtctgc
     2821 ggctcgtcca ggtgcaagaa aagaaacatt agagaattat attcgtcgat tagaacgttt
     2881 agaaacgtta tcagaaagtt atgatggtgt agaaaaagca tttgcgattc aggcaggtag
     2941 agaaatccga gtgattgtat ctcctgaaga aattgatgat ttaaaatctt atcgattggc
     3001 tagagatatt aaaaatcaga ttgaagatga attacaatat cctggtcata tcaaggtgac
     3061 agttgttcga gagactagag cagtagaata tgcgaaataa tttttgtctc cctcacaaat
     3121 tagtgaggga gcttttttaa gttgtagtct taatctagtt agacagcact ttatcggtaa
     3181 taactatatt aaacagtagt tatttgaaag taagacggac cttatattaa ataagaagtt
     3241 attgctttta ataaaaatgt tttaggcttc gtaattacta tatttatatt atgtaaacct
     3301 ataaagatga ttgcttttct atccaataaa aaagaagaga agatgtaaca catcttctct
     3361 tctgcaatat taattaggat ttatttctaa gttgagttat tttaattgta aatctgtttt
     3421 ctttaattct tttataactt ctgcagtatc ataacaattt gttgcaattg ttgaatatct
     3481 ctctgctaaa cgatatgcat taatgtaaag ctttaaactt tctttagcta tatcctctgc
     3541 atcttcgaat tttgatgggt tagacataac cactaattct gcaaattttt ctggatcaat
     3601 attaatagac atgtatttat ttacaactcc tatttatttt gatgtcttaa tactaacata
     3661 ttgaagtttt cagacaaagt aatgtctctc tataattgaa gaaaaataat tcaaagaatt
     3721 taatatttac tttaaatatt agaacgtatg aattaaacta gtatttatga gaggatgaac
     3781 aaaacatgag aataatgttt ataggggata tcgtaggtaa aattggacga gacgcaattg
     3841 aaacgtacat acctcaactg aagcaaaagt ataaaccaac agttacaatt gtaaatgctg
     3901 aaaatgcagc acatggtaaa ggtttgactg aaaaaatata taaacaatta ctaagaaatg
     3961 gtgtagattt catgactatg ggtaatcaca catatggtca acgtgaaatt tatgatttta
     4021 tagatgaagc aaaacgacta gtaagaccag cgaattttcc ggatgaagcg ccgggaattg
     4081 gtatgagatt tatacaaatt aatgatatta aacttgcagt tattaatctg caaggaagag
     4141 cgtttatgcc agatattgat gatcctttta aaaaggcaga tcaattagtc aaggaagcac
     4201 aagaacaaac tccgtttata tttgttgatt ttcatgcaga aacaacttct gaaaagtatg
     4261 caatgggatg gcatttagat ggtagagcta gcgctgttgt tggaacgcat acacacattc
     4321 aaacagcaga tgaacgtatt ttaccaaagg ggacagggta tataacggat gttggtatga
     4381 caggttttta tgatggcatt ttaggaataa ataaaacaga ggtaattgag cgttttatca
     4441 ctagtttgcc acaaagacat gttgttccaa atgaaggtag aagtgtatta tctggtgttg
     4501 ttattgattt agacaaagaa ggtaaaacaa agcacatcga acgtatattg ataaatgatg
     4561 accatccatt ttcaacattt taaaattacg taagtaaaca ttcgaattgg accctatcgt
     4621 ccattagtat gaatttaata tagtaccact gtttacatag taaatcggtg gttctttttg
     4681 ttatcattta atatgaaata tatccatagg aggcatataa ctatgaaacc acaattatcg
     4741 tggaaagttg gcggtcaaca aggcgaaggt attgaatcaa ctggggaaat cttcgctacg
     4801 gctatgaata gaaaaggata ttatttatat ggatatagac atttttcaag tcgtatcaaa
     4861 ggtggacata cgaataataa aattagagtt tctacgacgc ctgttcatgc aattagtgat
     4921 gatttagata ttttgattgc atttgaccaa gaaacaattg atgttaacca tcatgaaatg
     4981 agagaagaca gtattatttt agctgatgcc aaggctaaac ctgtgaaacc agaaggatgt
     5041 catgcacagc ttattgaatt accttttaca gcaaccgcta aagaattagg tacagcatta
     5101 atgaaaaaca tggttgcaat aggtgctact agcgcattga tgaatttgaa tacaaataca
     5161 tttgaagaac ttattactaa tatgttttct aaaaaaggtg acaaggtagt tgaagtcaat
     5221 atccaagcat taaacgaagg ttatcaatta atgcaatctc gcttacctga aatctacggg
     5281 gactttgaat tagagtcaac agatgcacta ccacatctat atatgattgg taacgatgcc
     5341 attggattag gtgcaattgc tgcaggttca caatttatgg cggcatatcc tattacacct
     5401 gcgtctgaag ttatggaata tatgattgcc aatatatcta aagtaaacgg agcggttatt
     5461 caaacagaag atgaaattgc tgctgtaact atggctattg gtgcaaatta tggtggagtc
     5521 agagcgttta cggctagtgc tggtccaggt ttatctttaa tgatggaagc aattggatta
     5581 tctggtatga ctgaaacgcc attagtcatt attaataccc aacgaggtgg accttctact
     5641 ggattaccta cgaaacaaga acagtcagat ttaatgcaaa tgatttatgg tacacatggt
     5701 gatattccaa aaattgttgt agcaccaaca gatgcagaag atgcatttta tttaactatg
     5761 gaagcattta atttagcaga acaatatcaa tgccctgtta tagttctaag tgatttgcaa
     5821 ttatctttag gtaaacaaac tgttgaaaaa ttagattata atcgtattga aattaaacgt
     5881 ggtgaaatca ttcaatctga tattgaacgt gaagaagatg ataaaggtta tttcaagcgt
     5941 tatgcgttaa catccgatgg tgtttctcct agacctatcc ccggtgttaa aggaggtatt
     6001 catcatataa ctggtgtgga acacaatgaa gaaggtaaac ctagtgaatc tgcgtcaaat
     6061 agacaacaac aaatggaaaa acgaatgcgt aaaattgagc agttactaat tgaatcgcca
     6121 gtagaagcta acttacaaca tgaggatgca gatattcttt atatcggttt tatttctaca
     6181 aaaggtgcaa ttcaagaagg tagtaaccgt ttgaatcaac aaggcataaa agttaacact
     6241 atacaaatta gacaattgca tccattccca acaagcgtta ttcaagatgc agttaataaa
     6301 gcgaagaaag tcgttgtagt ggagcacaat tatcaaggac aattggctag tattataaaa
     6361 atgaatgtca atattcatga taagattgaa aattatacaa agtatgatgg gacacctttc
     6421 ctaccacatg aaatcgaaga aaaaggcaaa ataattgcta ctgaaataaa ggagatggta
     6481 tagatggcga catttaaaga ttttagaaat aatgttaagc ctaactggtg ccccggatgt
     6541 ggcgatttct cagtacaagc tgcaattcaa aaagcagccg caaatatagg gttagaacct
     6601 gaagaagtag ctatcatcac cggtatagga tgttctggcc gtctttcagg atatattaat
     6661 tcttatggcg ttcattctat tcacggacgt gcattacctt tagctcaagg tgtaaaaatg
     6721 gcgaataaag atttaactgt tattgcatcg ggaggagatg gtgatggtta tgctataggt
     6781 atggggcata caatccatgc tttaagaaga aatatgaaca tgacgtatat agtcatggat
     6841 aatcaaattt atggtttgac aaagggacaa acatcgccgt catcagcagt aggatttgtt
     6901 actaaaacaa cgccaaaagg taatatagaa aaaaatgttg cgcctttaga attagcatta
     6961 tcatctggtg ccacatttgt agcccaaggt ttttcaagcg atattaaagg attaacaaaa
     7021 ctaattgaag atgcaattaa tcatgatgga ttttcattcg ttaatgtctt ttcaccatgt
     7081 gtgacttata ataaaattaa cacatacgat tggtttaaag aacatttaac aagtgttgat
     7141 gacattgaaa attatgattc tacagataaa caattagcga ctaaaactgt tattgaacat
     7201 gaatctttag taactggtat tgtttatcaa gataaagaaa caccatcata tgaatctcaa
     7261 attaaagagt tagatgatac accacttgct aaaagagata tcaaaattac tgaagacacc
     7321 ttcaatgcat taactgaaca atttatttaa taattaaaac tgagtcgtat aaatgtattt
     7381 ataacagatc catttatgct actcagtttt ttactattac aaaaaataaa ggagttttta
     7441 aaaatgaaag acacattaat gagtatacaa ataattccta aaacaccaaa caatgacaat
     7501 gttatacctt acgtagacga ggcgattaaa ataattgacg aatctggttt gcattttaga
     7561 gtaggtccgt tagaaacgac agtacaagga aatatgaatg aatgtttaat tttaatacaa
     7621 tcattaaatg aacgaatggt ggaacttgaa tgtccaagta ttattagcca agttaagttt
     7681 tatcatgtgc cagatggcat cactattgaa actttaactg aaaaatatga tgaataacat
     7741 taaaagtgaa gtaaactgga tttgaattgg cttgttagag atgacgtata actttaactg
     7801 tttttgcact ttatagttaa atttaatata attattaaat gatacgggca aatagaaagg
     7861 attttgtaaa gtgaacgaag aacaaagaaa agcaagttct gtagatgttt tagctgagag
     7921 agataagaaa gcagaaaaag attatagtaa atattttgaa catgtttatc agccgcctaa
     7981 tttaaaagaa gcgaaaaaaa gaggtaaaca agaagttcgt tataatagag atttccaaat
     8041 tgatgaaaaa tatcgcggta tggggaacga gcgtacattt ttaattaaaa catatggatg
     8101 tcaaatgaat gcacatgaca ctgaggtcat tgctggtata cttgaagcat taggctatca
     8161 agcaacgact gatattaaca ctgcagatgt tattttaatt aatacatgtg cgattagaga
     8221 aaatgccgag aacaaagtgt ttagtgaaat aggtaatttg aagcatttga aaaaagaacg
     8281 acctgatatt ttaatcggtg tttgtggttg tatgtcacaa gaagagtcag tagtgaataa
     8341 aattttaaaa tcgtatcaaa atgtagatat gatatttggt acacataata ttcatcattt
     8401 accagaaatt ttagaagaag catacttatc taaagcaatg gttgttgaag tatggtctaa
     8461 agaaggagac gttattgaaa atcttccaaa agtccgtgaa ggcaacatta aagcatgggt
     8521 caatattatg tatggttgtg ataagttttg tacatattgt attgttccat ttacaagagg
     8581 taaagaacga agccgtagac ctgaagacat tatagatgaa gtacgtgaac ttgctcgtga
     8641 aggttacaaa gaaataacgc ttttaggtca aaatgtaaat tcttatggta aagatttaca
     8701 ggatatagaa tatgacttag gagatctttt acaagcaatt tctaaaatag cgattccaag
     8761 agttcgtttc acaacaagtc atccttggga ctttacagat cacatgattg atgttatttc
     8821 agagggtggt aatatcgttc cgcatatcca cttgccagtt caatctggaa ataatgcagt
     8881 attaaaaata atgggtagaa aatatacacg agaaagttat ttggatttag taaaacgaat
     8941 caaagataga attcctaatg tagcattaac tacagatatt attgtagggt atccaaatga
     9001 atcagaggaa caatttgaag aaactttaac tctgtatgat gaagttggtt ttgaacatgc
     9061 atatacgtac ttgtattcac aacgtgatgg tacgcctgct gctaaaatga aagataatgt
     9121 acctttaaat gtcaaaaagg aacgattgca acgtttgaat aaaaaagttg gtcattattc
     9181 acaaatagct atgagtaagt acgaaggaca aactgtaaca gtactttgtg aaggaagtag
     9241 taaaaaagat gatcaggttc ttgctggcta cactgataaa aataagctag ttaatttcaa
     9301 agcgcctaaa gaaatgattg gtaaactagt ggaagtacga atagatgaag ctaaacagta
     9361 ttcattaaat ggcagtttta taaaggaagt agagccggaa atggtgattc aataaatgta
     9421 taataaagat gacgtgttga accaagcgga taatattgca aataaaatta aaaatttgga
     9481 tactatcaaa acatatcaac aaattgaagc acagattcat cagaaccaaa cgataaagac
     9541 taaaatggat atgttaaaaa agcatcaaaa acaagcagta aactttcaaa attacgggaa
     9601 acaaaatgcg ctagaacagt cggaacatac cattcagagt atagaagcag aaataaatac
     9661 attgcccata gttgaacagt ttcaaacttc acaatatgaa gcgaatcaat tattgaaaat
     9721 gtttgtatca acaatggaaa cacgtttaaa tgaccataat aaagccaagc atagtgatta
     9781 attacaaaca aaaaggagaa caatcatatg aaatcaagaa aactggctat aactgcactt
     9841 ttaattgcaa taaatgttgt attaagcagt attatcatca ttcctctagg accagttaag
     9901 gcagcaccag tacagcattt tgtaaatgta ttaagtgcgg tcatagtagg tccttggtat
     9961 ggattagctc aagcgcttat atcatcaatt ttaagagttc tttttggtac tggtacagct
    10021 tttgcatttc cgggtagtat gattggagtt ttattggcta gtatgtttta catatatcgt
    10081 aaacatatat ttatggccgc ggtcggtgaa gtacttggaa ctggtgtcat cggaagttta
    10141 atttgtatac cattagcata tttccttggg cttcaagact tcttcattaa accgttaatg
    10201 attacgttca tagtctcaag tgctatcgga tctattataa gttatttctt attaattact
    10261 ctaaaaaaac gtggtattct tcaaaggttt ataaaataat tttatgatat atgatttcaa
    10321 acagagtcat tgttcatttt aatattgaac gcccttactt aattaaatgt cttgaatgtt
    10381 agaattttaa tttaattcta aacatgctat tgacacattt tattaggttt gaggcgtttt
    10441 cttatacat
//
LOCUS       DAMQYT010000079        10238 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMQYT010000079 DAMQYT010000000
VERSION     DAMQYT010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10238
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.079"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            267..1721
                     /locus_tag="PGA33_001508"
     CDS             267..1721
                     /locus_tag="PGA33_001508"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDG6473317.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            2144..2791
                     /gene="cysE"
                     /locus_tag="PGA33_001509"
     CDS             2144..2791
                     /gene="cysE"
                     /locus_tag="PGA33_001509"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDG6473318.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            2775..4175
                     /locus_tag="PGA33_001510"
     CDS             2775..4175
                     /locus_tag="PGA33_001510"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDG6473319.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            4168..4572
                     /locus_tag="PGA33_001511"
     CDS             4168..4572
                     /locus_tag="PGA33_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDG6473320.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            4580..5326
                     /gene="rlmB"
                     /locus_tag="PGA33_001512"
     CDS             4580..5326
                     /gene="rlmB"
                     /locus_tag="PGA33_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDG6473321.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLLTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            5326..5850
                     /locus_tag="PGA33_001513"
     CDS             5326..5850
                     /locus_tag="PGA33_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDG6473322.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            5931..6500
                     /locus_tag="PGA33_001514"
     CDS             5931..6500
                     /locus_tag="PGA33_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDG6473323.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            6615..6758
                     /gene="rpmG"
                     /locus_tag="PGA33_001515"
     CDS             6615..6758
                     /gene="rpmG"
                     /locus_tag="PGA33_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDG6473324.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            6814..6996
                     /gene="secE"
                     /locus_tag="PGA33_001516"
     CDS             6814..6996
                     /gene="secE"
                     /locus_tag="PGA33_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDG6473325.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFR"
     gene            7009..7557
                     /gene="nusG"
                     /locus_tag="PGA33_001517"
     CDS             7009..7557
                     /gene="nusG"
                     /locus_tag="PGA33_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDG6473326.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            7738..8160
                     /gene="rplK"
                     /locus_tag="PGA33_001518"
     CDS             7738..8160
                     /gene="rplK"
                     /locus_tag="PGA33_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDG6473327.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            8368..9060
                     /gene="rplA"
                     /locus_tag="PGA33_001519"
     CDS             8368..9060
                     /gene="rplA"
                     /locus_tag="PGA33_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDG6473328.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
     misc_feature    9148..9294
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            9332..9832
                     /locus_tag="PGA33_001520"
     CDS             9332..9832
                     /locus_tag="PGA33_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDG6473329.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     gene            9875..>10238
                     /gene="rplL"
                     /locus_tag="PGA33_001521"
     CDS             9875..>10238
                     /gene="rplL"
                     /locus_tag="PGA33_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDG6473330.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVEL"
BASE COUNT         3825 a         1401 c         2023 g         2989 t
ORIGIN      
        1 tgtttatgta gtgcagtctt gattcttagg gatagctttt ttaaagtgtt gaaaaaacaa
       61 cagctttcta tttaaaattt aagcatatta cccagggaat tatgttatga ttaaacaatg
      121 aaattatttt aaggtcatgt taaaaagcat tgtttcgttt taacaataag acgagttaga
      181 catttgttat gacggtcgaa gatagcaaat gaacaattta tatgaactaa cttgaaaatc
      241 tgatatttaa tttaggagtg aaaaaaatga gcgatcgtat aagagtaaga tatgcaccaa
      301 gtccaactgg gtatcttcat attggtaatg caagaacagc attattcaat tacttgtatg
      361 ctaaacatta caacggagat tttgtgattc gaattgaaga tactgataaa aaacgtaatt
      421 tagaagatgg agaaacatca caatttgata atcttaaatg gttaggatta gattgggatg
      481 agtctgtaga taaagacaat ggctacggac catatcgtca atctgaacgt caacatatct
      541 accaaccatt aatagatcag ttactagcag aagataaagc atataaatgc tatatgacag
      601 aagaagaatt agaagctgaa cgtgaagcgc aaatcgctcg tggtgaaatg cctcgctatg
      661 gtggtcaaca tgcgcatttg actgaagaac aacgtcaaca atttgaagca gaaggacgcc
      721 aaccatcaat tcgtttccga gtacctcaaa accaaacgta ttcatttgat gatatggtaa
      781 aaggaaatat ttcatttgat tcaaatggta ttggtgactg ggttatcgta aaaaaagatg
      841 gcattccaac gtacaatttt gcagtagcta tagatgatca ttacatgcaa atttcagatg
      901 taattcgtgg tgatgatcat atttcaaaca cgcctaaaca aattatgatt tatgaagcat
      961 ttggctggga gccacctcgt tttggtcata tgtcattaat tgttaatgaa gaacgtaaaa
     1021 agttaagtaa acgtgatggg caaattttac aatttattga gcaatatcgt gacttaggtt
     1081 atttacctga agcgttattt aattttattg cgttattagg ttggtctcct gaaggtgaag
     1141 aagaaatctt ttctaaagaa gaatttatca aaatctttga tgaaaagcgt ttgtcaaaat
     1201 caccagcatt tttcgataag caaaaattag catgggttaa taaccaatat atgaaacaaa
     1261 aagatactga aacagtattc caattagcat tacctcattt aattaaagca aatttgattc
     1321 ctgaggtgcc gtcagaagag gatttatctt ggggacgcaa attaattgcg ctttatcaaa
     1381 aagaaatgag ttatgccggt gaaattgtac ctttatcaga aatgttcttt aaagaaatgc
     1441 cagctcttgg tgaagaagaa caacaagtga ttaatggaga gcaagtacca gagttaatga
     1501 cgcacttatt cagtaaatta gaagcacttg aaccatttga agcggctgaa attaaaaaga
     1561 caattaaaga agttcaaaaa gaaacaggaa taaaaggcaa gcaattattt atgcctattc
     1621 gtgttgctgt aacaggccaa atgcatggtc ctgaattacc aaatacaatt gaagtacttg
     1681 gtaaagaaaa agtgctaaac cgtttaaaac aatataagta atgaaataga caagatcaag
     1741 gtagtatata cttgaatttt ataacgtaac cacatatgat aaagttgtat taacaaatta
     1801 caacaatatg aataaagtat tttatataaa acgaaggatt agtaattaaa tttatacgat
     1861 gcagagagtg tacggttgct gtgagtacaa cgtagaaatt aatgaatgca ccttcgtaaa
     1921 atgaattaaa tatataatga gagtgatgag cattaagttg acttagtttc cttgataatt
     1981 tggaagcgcc cgcaatatta ttaatgttat tcgctaaatt cagagtggaa ccgtgcggaa
     2041 gcgcctctaa caatacaact tgtatgttag tggtgctttt ttgatattta atttcgcagg
     2101 tacttcaatt atgtatttac gtagttcaat cattgaggag gaaatgatct tgttaaaaag
     2161 aatgagagac gatataaaaa tggtatttga gcaggatcca gcggcacgtt caacattaga
     2221 agtcattaca acgtatgcag gtttacatgc agtttggagt catttgattg cacataagtt
     2281 atacaaccaa aaaaaatatg ttgcagcacg cgcgatatct caaatttcaa gatttttcac
     2341 aggtatagaa atccatccag gtgctaaaat tggaaagcgt ctatttatag atcatggtat
     2401 gggcgttgta ataggagaaa catgtacaat tggtgataat gtgacaatct atcaaggcgt
     2461 gacacttggt gggacaggga aagaaagagg gaaaagacac ccagatatag gagacaatgt
     2521 tttaatagca gccggtgcga aagttttagg aaatattaaa ataaattcaa atgtaaatat
     2581 tggtgcaaat tcagttgttt tacaatcagt tccaagttat tcaacggttg ttggtatacc
     2641 aggacatatt gttaagcaag atggtgttcg agttggaaaa acatttgatc atcgccatct
     2701 acctgatcca atttatgaac aaattaagca tttagaacga caacttgaaa agactaggaa
     2761 tggagagatt caagatgatt acattatata atacgcttac acgtcaaaaa gaagtgttca
     2821 agcctataga accagggaaa gtaaaaatgt atgtatgtgg tcctactgta tataactaca
     2881 ttcatattgg taacgcaaga ccagcaatta attatgacgt agtgagacgt tactttgaat
     2941 accaaggata taatgtagaa tatgtatcaa attttacaga cgtagatgat aaattaatta
     3001 aacgttctca agaattaaat cagtctgttc ccgaaattgc agaaaaatat atcgctgctt
     3061 ttcatgaaga tgttggtgcg ttaaatgtta gaaaagcgac ttcaaatcca agggtaatgg
     3121 accatatgga tgacattatt caatttatta aagatttggt ggatcaaggt tatgcatatg
     3181 aaagtggtgg cgatgtttac tttagaacac gtaaatttga aggttatggt aaattaagtc
     3241 atcaatccat agatgactta aaagtgggtg ctcgtataga tgcaggagag cataaagaag
     3301 atgcacttga ttttacattg tggaaaaaag cgaagcctgg cgagattagt tgggatagcc
     3361 catttggtga aggtagacca ggatggcata tagaatgttc tgtaatggca tttcatgagc
     3421 taggacctac aattgatata catgcgggtg gttcagattt acaatttcca catcatgaaa
     3481 atgaaatagc acaatcagaa gcacataatc atgcgccatt tgctaattat tggatgcata
     3541 atggtttcat taatattgat aatgaaaaaa tgagtaaatc actaggcaac tttattttag
     3601 ttcacgatat tattaaagaa gttgatccag atgtactaag attctttatg attagcgtac
     3661 attatagaag cccaattaac tataatctag aattggtaga atcagcacgt agtggactag
     3721 agcgtattcg caatagttat caattaattg aagagcgcgc acaaattgct actaatattg
     3781 aaaatcaaca gacatatatt gatcaaattg atgcgatttt aaatcgtttt gaaacagtta
     3841 tgaatgatga ttttaataca gctaatgcaa ttacagcttg gtatgattta gcaaaacttg
     3901 cgaataaata tgtactagag aacacaacat caacagaagt aattgataaa tttaaagcag
     3961 tttatcaaat tttcagcgat gttttaggtg taccgttaaa atctaaaaat gcagatgaat
     4021 tattggatga agatgttgaa aaattaatcg aagagcgtaa tgaagcaagg aaaaacaaag
     4081 attttgcacg agcagatgaa attcgagaca tgctgaaatc acaaaacatt atattagaag
     4141 acacacctca aggggttaga tttaaacgtg gataatcaac aagataatca cattaaatta
     4201 ttgaatccat tgaccttagc atatatggga gacgcagtct tagatcaata tgtacgtacc
     4261 tatatcgttt taaagcttaa aagtaagcct aataaactac atcaaatgtc taaaaaatat
     4321 gtatctgcca aaagtcaggc gcaaacgtta gaatatttaa tggagcaaga atggtttaca
     4381 gacgaagaaa tggatatttt gaagcgaggg cgtaacgcga aaagtcatac taaagctaaa
     4441 aacactgatg ttcaaacata tcgtaaaagt tcagcgatag aagcagtgat aggttttctt
     4501 tatttagaaa aaagagaaga acgattagag gcattattaa ataaaataat aacaatagta
     4561 aacgaaaggt agtgacgatg tggaagatac ggttattgtt ggtaggcatg ctgttagaga
     4621 agcgattatt actgggcatc cgataaataa gatattgatt caagaaggta ttaaaaagca
     4681 acaaattaat gaaattttaa aaaatgcaaa agatcaaaaa atcattgttc aaactgtacc
     4741 aaaatctaaa ttagattttt tagcaaatgc accacatcag ggtgttgcag cgcttattgc
     4801 accatatgaa tatgctgact tcgatcaatt tttaaaacag caaaaagaaa aagaaggttt
     4861 attgacagta cttatattag acggcttaga agacccacat aacttgggat caattttaag
     4921 aacagccgat gcaacgggag ttgatggtgt tattattcct aaacgtcgtt cagttacact
     4981 aacgcaaaca gttgcaaaag cctcaacagg tgcaattgaa catgtaccag ttattcgagt
     5041 gacaaattta gctaaaacta tcgatgaact aaaagataat ggcttttggg tagctggcac
     5101 tgaagctaat aatgcaacag attatagaaa tctagaagcg gacatgtcat tggctattgt
     5161 aattggtagc gaaggacagg gtatgagtcg cctagtaagt gataaatgcg atttttatat
     5221 taagattcca atggttggac atgtaaacag tttgaatgct tcggttgcag caagtttaat
     5281 gatgtacgaa gtatttcgaa aaagacatga tgttggagaa atataatgaa agaacgttac
     5341 ttaatcattg atggatacaa tatgatagga caatcaccaa cgctaagcgc cattgcaaaa
     5401 gagaatttag aagaagctag aatgcaatta atagatgcaa ttgcaaatta taatgcagtt
     5461 atttcagatg aaattatttg tgttttcgat gcttatgacc aatcgggtgt tgaaagagaa
     5521 tacatgtatc atggcgttaa aacgattttt accaaggaaa aagaaacagc tgatagtttc
     5581 atagaacgtt atgtttatga actttatgac aagcatacta agcatattac agttgtaaca
     5641 agtgatatga gtgagcaaca tgctatcttt ggatcaggtg catatagaat atcatctcgc
     5701 gaaatgtgga gagatttaaa agaaaatgaa attgatgtga gtaaatcatt agatgatata
     5761 agtgaaaaca agccaagaac tcgaattccg ttatcttctg aaatccttgc agaatttgaa
     5821 aaaatacgaa gaggacatca taagaaatga catttccgtc atcttgaaat tttgaatgta
     5881 acaatattaa actaacctta aattttagat agaaggggtt agtttaatat ttgaaatacg
     5941 atttgacaac tcaagacagt acaatcaaac gtaacaatgc aataaatgat aaagacttcg
     6001 aaaagttagt aatggatcta caaccattaa ttattcgacg catcaaaaca tttggattta
     6061 atcattatga tttagaagac ttatatcaag aaatacttat acggatgtat aggtcggtcc
     6121 aaacatttga ttttagtgga gagcagcctt tcacaaatta tgttcaatgt ttaattacgt
     6181 ctgtaaagta tgattatttg agaaaatatt tagctacaaa taaaagaatg gataatttga
     6241 ttaatgaata tagagttacg tatccatgtg caataaagcg ttttgatgtt gaaaacaatt
     6301 atttgaataa attagcaatt aaagagttga tttgtcagtt taagtatttg agtgcatttg
     6361 aaaaagatgt catgtattta atgtgtgaac aatataagcc gagagaaatt gctcaattga
     6421 tgcatgtaaa agagaaagtg atttataatg ccatacaacg atgtaaaaat aaaataaaac
     6481 gttatttcaa aatgatttga aaagcgcctt aggacgtgaa ttgaattata acgtgttact
     6541 tactgatggt ttgacatttg ttataaattt tatgtatagt atactggtat tataatgaat
     6601 aaaggtgaat tattgtgaga aaaatacctt taaattgtga agcttgtggc aatagaaatt
     6661 ataatgttcc taagcaagaa ggctcggcaa caagattaac cttaaagaaa tattgtccaa
     6721 aatgtaacgc gcacacaatt cataaagaat cgaaataaat acattcgaaa taatactttg
     6781 ataatatgtt caaaggattt ggaggttgag cagatggcta aaaaagaaag tttctttaaa
     6841 ggcgttaagt ctgaaatgga aaaaacaagt tggccgacga aagaagagct atttaaatat
     6901 actgtaattg tagtttctac tgttatattc ttcttagtct ttttctatgc cttagattta
     6961 ggaattacag cattgaaaaa tttattattt cgttagagga gtgaagacat gtctgaagaa
     7021 gttggcgcaa agcgttggta tgcagtgcat acatattctg gatatgaaaa taaagttaaa
     7081 aagaatttag aaaaaagagt agaatctatg aatatgactg aacaaatctt tagagtagtc
     7141 ataccggaag aagaagaaac tcaagtaaaa gatggcaaag ctaaaacgac tgttaaaaaa
     7201 acattccctg gatatgtttt agtggaatta atcatgacag atgaatcatg gtatgtggta
     7261 agaaatacac caggcgttac tggttttgta ggttctgcag gtgcagggtc taagccaaat
     7321 ccattgttac cagaagaagt tcgcttcatc ttaaaacaaa tgggtcttaa agaaaagact
     7381 atcgatgttg aactcgaagt tggcgagcaa gttcgtatta aatcaggtcc atttgcgaat
     7441 caagttggtg aagttcaaga aattgaaaca gataagttta agctaacagt attagtagat
     7501 atgtttggcc gagaaacacc agtagaagtt gaattcgatc aaatagaaaa gctttaatta
     7561 acaattaaag ttattaaact aaccaaaaga taaaaaagag tattgatttt ttaattagaa
     7621 aagtgttaaa attatgtggt cgcgctttta gagcgcccat ttcgtcacga aatgttaaga
     7681 gtgggagggc aaaactgagc cctgtgacca catcacgata tcaaggaggt gcacatcgtg
     7741 gctaaaaaag tagataaagt tgttaaatta caaattcctg caggtaaagc gaatccagca
     7801 ccaccagttg gtccagcatt aggtcaagca ggtgtgaaca tcatgggatt ctgtaaagag
     7861 ttcaatgcac gtactcaaga tcaagcaggt ttaattattc cggtagaaat cagtgtttat
     7921 gaagatcgtt catttacatt tattacaaaa actccaccgg ctccagtatt acttaaaaaa
     7981 gcagctggta ttgaaaaagg ttcaggcgaa ccaaacaaaa ctaaagttgc tacagtaact
     8041 aaagatcaag tacgcgaaat tgctaacagc aaaatgcaag acttaaacgc tgctgacgaa
     8101 gaagcagcta tgcgtattat cgaaggtact gcacgtagta tgggtatcgt tgtagaataa
     8161 ttttacgaat attaaatttg attacatgat ttaaacgatg aagcagataa cagagataat
     8221 aatgatgaat tataaatata atctgaatga ctagattaat gattgattta ttcataagat
     8281 taattcttct gttgtctgct cttaacttgc atatagcaag taatgtggga ggaaattccg
     8341 ctaaaaccac taaaggagga actataaatg gctaaaaaag gtaaaaagta tcaagaagca
     8401 gctagtaaag ttgaccgtac tcagcactac agtgttgaag aagcaattaa attagctaaa
     8461 gaaacaagca ttgctaactt tgacgcttct gttgaagttg cattccgttt aggaattgat
     8521 acacgtaaaa atgaccaaca aatccgtggt gcagttgtat taccaaacgg aactggtaaa
     8581 tcacaaagtg tattagtatt cgctaaaggt gacaaaattg ctgaagctga agcagcaggt
     8641 gctgactatg taggtgaagc agaatacgtt caaaaaatcc aacaaggttg gttcgacttc
     8701 gatgtagtag ttgctacacc agacatgatg ggtgaagttg gtaaattagg tcgtgtatta
     8761 ggaccaaaag gtttaatgcc aaaccctaaa actggaactg taacaatgga tgttaaaaaa
     8821 gctgttgaag aaatcaaagc tggtaaagta gaatatcgtg ctgaaaaagc tggtatcgta
     8881 catgcatcaa ttggtaaagt ttcatttact gatgaacaat taattgaaaa cttcaatact
     8941 ttacaagatg tattagctaa agctaaacca tcatctgcta aaggtacata cttcaaatct
     9001 gttgctgtaa ctacaacaat gggtcctgga gttaaaattg atactgcaag tttcaaataa
     9061 taaatgatat aaacaattac aggctgaaag aaatatcttt cagtctgtaa aaatatattg
     9121 acaataagta atttccaagt tatattactt attgtgatta ttttacctaa gacagtagga
     9181 gttatttata acttaaaatt tatcctgccg aggctaaaat tgacttgaac gtgatgatct
     9241 atgatctttc aagcactttt tgccgtgggt agaaagtgct ttttttatta attttaaaaa
     9301 aagcaccaaa aatttaaatg gaggtgtctg aatgtctgct atcattgaag ctaaaaaaca
     9361 actagttgat gaaattgctg aggtactatc aaattcagtt tcaacagtaa tcgttgacta
     9421 ccgtggatta acagtagctg aagttactga cttacgttca caattacgtg aagctggtgt
     9481 tgagtataaa gtatacaaaa acactatggt acgtcgtgca gctgaaaaag ctggtatcga
     9541 aggcttagat gaattcttaa caggtcctac tgctattgca acttcaagtg aagatgctgt
     9601 agctgcagcg aaagtaattt ctggatttgc taaagatcat gaagcattag aaattaaatc
     9661 aggcgttatg gaaggcaatg ttattacagc agaagaagtt aaaactgttg gttcattacc
     9721 ttcacacgat ggtcttgtat ctatgctttt atcagtatta caagctcctg tacgcaactt
     9781 cgcttatgcg gttaaagcta ttggagaaca aaaagaagaa aacgctgaat aatttttagc
     9841 gtaaaaaaat taaaaataat ggaggaatta taaaatggct aatcatgaac aaatcattga
     9901 agcgattaaa gaaatgtcag tattagaatt aaacgactta gtaaaagcaa ttgaagaaga
     9961 atttggtgta actgcagctg ctccagtagc agtagcaggt gcagctggtg gcgctgacgc
    10021 tgcagcagaa aaaactgaat ttgacgttga gttaacttca gctggttcat ctaaaatcaa
    10081 agttgttaaa gctgttaaag aagcaactgg tttaggatta aaagatgcta aagaattagt
    10141 agacggagct cctaaagtaa tcaaagaagc tttacctaaa gaagaagctg aaaaacttaa
    10201 agaacaatta gaagaagttg gagctactgt agaattaa
//
LOCUS       DAMQYT010000080        10237 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMQYT010000080 DAMQYT010000000
VERSION     DAMQYT010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10237
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.080"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(941..1135)
                     /locus_tag="PGA33_001522"
     CDS             complement(941..1135)
                     /locus_tag="PGA33_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473331.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYC
                     LLPLIVNLSIYGFYVFVKRK"
     gene            1245..1937
                     /locus_tag="PGA33_001523"
     CDS             1245..1937
                     /locus_tag="PGA33_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473332.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKS
                     KNIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEAN
                     DKNAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKN
                     YVEYKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAV
                     CGTAFAAGCSVG"
     gene            complement(2105..2254)
                     /locus_tag="PGA33_001524"
     CDS             complement(2105..2254)
                     /locus_tag="PGA33_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473333.1"
                     /translation="MKIKTFATFTHPQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIV
                     FYEDK"
     gene            complement(2801..3117)
                     /locus_tag="PGA33_001525"
                     /pseudo
     CDS             complement(2801..3117)
                     /locus_tag="PGA33_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
     gene            complement(3110..4093)
                     /locus_tag="PGA33_001526"
     CDS             complement(3110..4093)
                     /locus_tag="PGA33_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDG6473334.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPE
                     VRKSNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQ
                     EFPEQLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLK
                     KAIHDRGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
     gene            4519..5262
                     /locus_tag="PGA33_001527"
     CDS             4519..5262
                     /locus_tag="PGA33_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDG6473335.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            complement(5489..5674)
                     /locus_tag="PGA33_001528"
     CDS             complement(5489..5674)
                     /locus_tag="PGA33_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDG6473336.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(5679..6017)
                     /locus_tag="PGA33_001529"
     CDS             complement(5679..6017)
                     /locus_tag="PGA33_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473337.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            complement(6263..6871)
                     /locus_tag="PGA33_001530"
     CDS             complement(6263..6871)
                     /locus_tag="PGA33_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDG6473338.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            complement(7357..8256)
                     /locus_tag="PGA33_001531"
     CDS             complement(7357..8256)
                     /locus_tag="PGA33_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HDG6473339.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            complement(8355..9821)
                     /gene="msr(A)"
                     /locus_tag="PGA33_001532"
     CDS             complement(8355..9821)
                     /gene="msr(A)"
                     /locus_tag="PGA33_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HDG6473340.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
BASE COUNT         3471 a         1581 c         1429 g         3756 t
ORIGIN      
        1 ttagttaagg gttcttctca acatcaataa attttttcgg cataaatgcg tgtctatgcg
       61 tagacatgga gacacttcag aattatggag tgattacatg agtttaacta tttttcttta
      121 ttcagtttta ggaagtttat tagcaaatta tatctacgaa aaatcttcaa aataaataac
      181 tttttcggtt ctactttgta cactttatct acctggtaga cctaattgtt attgctttac
      241 aggtctaaaa ttcgtcgtta taatctagtt aaatcccgta agggcgcact tatacgtcgg
      301 ataaatcgac atgtgcaaaa acgtaatcga ccttcgtcga tttttttatt tcaaaattaa
      361 agaaacggct tgagatattc cgagcagtta taaaaattaa tttgcgttga atattgctac
      421 tacattcttg aaaaagtgat gtagatttat agcccataac ttttttgaat tacatcgacg
      481 atacggcgtc gaaagaaaaa taaatagacc gtatgaaaga aaaagctacc tcactttatc
      541 tggggtagct tttatttttt atgcccttat tttgaatttt aagagggcat tttaaagatt
      601 taggggtgat tcatatagtt ttatatctaa aacttatata tgcttttaaa acgcaaatat
      661 gagccaaata aatatatgct attttcaaaa acaaaaattt gagcaaaaac aataactatt
      721 tttagacact gcccatttac atgcaaatta aaaattagca taaaattctt taaaacttcc
      781 ccacgttttt ctaaattcct gtagggcgtt tttatcaaaa atcaagacaa gaagaaactc
      841 gttcacactc gtttcaaaaa aacaagaaat ctaatctata cacctaccgt tttccaaccc
      901 cctcaaagca tacaaattaa aaacaaaatt attcataatc ttactttctt ttaacaaaaa
      961 cataaaaacc ataaatagac aaattaacta tcaaaggcaa taaacagtat aaaaaaacca
     1021 cgaataaatc ttcatcaaca tctaaaataa aaaagaataa aacaattaac aaaatactaa
     1081 ttaacaaacc taaaagtggc aaacttttta tatatttttc taaattttca ttcataaaat
     1141 agactccttt caaaaatatg tttataaata tattaaaata aatattgatt ttataaagcg
     1201 cttacttata caattaaaat atcttattat cttggaggta tcaaatgaaa attataaaat
     1261 tattttcaat aatttccttg ttcctactgg tatttagcgc gaatttttct aatgcttacg
     1321 caaatgaaga acaaaaaagt tctttactag aaaatcaaaa agaaaaattg aaaagcaaaa
     1381 acatcgaagt atcacaaatg accaagaaag aaataagttt tctagaacaa ttgcaaaata
     1441 aaaatgaaga tatgcaaaaa ttcgaaaagc aaatgaaaaa agaaggattt aaaaaaataa
     1501 agctagaaga caaaaattct aaattaaaat atctagctac agaagcaaat gataaaaatg
     1561 catatggcta catagctaat aacgcttata aaaatgaaaa aacaaaagaa attgtagttt
     1621 ctgaaacaat atatgataac tacaacaaaa aaatcacaaa attcattgcc gaaaaaagaa
     1681 gtatgactga aaacaaagag ggaaaaatac ttattaacaa aaattacgtt gaatataagg
     1741 ctgaaaaagt tgataaaaac agaccggtaa catatgcagg attcaaatgg aacggcaaag
     1801 cattcgcttg tagtatggct ggactttatg catgtgctca ctactgtgga gtatgggcaa
     1861 tcgtaaatcc aatagccggc ggtgtatgtg aagcagtttg cggaactgca tttgcagctg
     1921 gatgctctgt aggttgattt tattccaatt gttttattga cgttgagcct cggaaccctt
     1981 aacaatccca aaacttgtcg aatggtcggc ttaatagctc acgctatgcc gacattcgtc
     2041 tgcaagttta gttaagggtt cttctcaaca tcaataaatt ttttcggcat aaatacgatg
     2101 ttccttattt atcctcataa aaaacaatag ctgaaatata taaccaatta ctgtgtgttt
     2161 gaatttctaa tacatgaata tcattcttta aaaacctatt aacttgttta tctaattgag
     2221 gatgagtaaa agtagcgaaa gttttaatct tcatgatatc tctcctttta taaaaatagc
     2281 aatccttatt acaatattaa acacttttat tttggatttt aagaggcatt ttgaagactt
     2341 agaggcaaat catataggtt ttgtacttaa aaatctatac agtctcttaa aacgcaaata
     2401 tgagccaaat aaatatatgc taatttcaca aacaaaaatt tcagcaaaat cagtgactaa
     2461 ttttttagat actgcccatt tacatgcaaa tttaaaaatt agcataaaat actttaaaac
     2521 ttccccatgt ttttctaaat tcctgcaggg cttttcttat caaaaactta gaaattttaa
     2581 tttaactcta aaatctccgt aagagcataa gggaaatttg ctatcattca aaaatgatag
     2641 atctggcaag gccagacatt ccaagggttt taagttattt taaagtgata tcatacaaag
     2701 ctgtagtgtc acacttttgt gacactacat acagcatttg tcacaaaaat aagacagcac
     2761 ttttttacag aaaaacaata aaaaaagaca tcagttatca ctaatgtctt aaataataat
     2821 tttgcttttc gaaattccaa attgcggtaa ttgaatgtgc tttttttctt cgtctgtcgt
     2881 tttatcttca tcacttgtat taatcaaatt accatcttcg gcatcgtcta agtttaaaat
     2941 tttatgcttt tgtttaagca atccaccata acttaacata cgttttcgat acaaaccttt
     3001 ttctaaatca cttacaattt cttgattttt ttcgtcgtca tcagttaaaa agtcagatga
     3061 tttaacggaa tattttgatg tctcttcgag tgttataaaa tcatttgaat tattgattaa
     3121 agatattata ttgtttcatg tttttacggc gcatataaag ggcatgtgtt tcttcaagtt
     3181 cagttagaat tgtttgcatt aaataagctt gcattgactt gattgtttcc ttttggcctt
     3241 taactcccct atcatgtata gcttttttaa gtctttttaa agaagttaat atttcatcct
     3301 caatgtcctc taacatgtaa tgcatatctt catgccttga attaaatgat ttttttgctt
     3361 taagaataac agcctttatg atttctaaat ctctataatc atattttaat aaataattag
     3421 ttagttgttc tgggaattct tgcaatagca tttctttatc atcaaaatta tgtgtaaatt
     3481 gatttgaatg atttgaatga ttactaattt catttttgta ctttatatca ttcaagtcat
     3541 tcatatcatt attctcagta tcattaaagt caatgtcatt aaagtcagta tcattagtgt
     3601 tcgattttcg aacttctgga cgttcgattt tcgaacttct ggacgttcga ttttcgaact
     3661 tctggacgtt cgattttcga acttccttct cgccgtaagg ttcaacatcg ttttcttctt
     3721 tttctatttt gtaaatatca cgttctgtga caataggttt cattaaatat agaatatttg
     3781 gtttatttaa accacgtcgt ttttgaatga gtagaccatc gttttctaac ccttttttta
     3841 tttttgttaa cttttctttt ttacaattta agatttccat tagcttttca tttgaataaa
     3901 caaagtaaat gttaccatct tcatcaaccc agctattttt aatagataaa tttaagcgat
     3961 cacgtaagat agcataggct attttcatat cattagttag atttttataa ttttcactgg
     4021 taaagaatac tttagggatt tgataaaatc tttctttgta attttcttgc actgtaaaat
     4081 attgattttg catgacaaaa aacctctttc catattttag tttggtaaga ggtaataaaa
     4141 cgtttttatt tagctttaca tttatttaat gatttaggtg cttgcactaa gatattattc
     4201 attatataat acaagtatcg aaaattatat aaataaacag tttctaaacg ttttattacc
     4261 aaaccccgac tattaccagt agttggggtt tttgttatat taagtttatt atagcacttt
     4321 aaataaactt ataaaaggga aaatatagct ttatcaaggg tttataaacc cttgataaat
     4381 atcataaacc tttcataaac ccttgataaa aaggttgatg gatgtttatt tttatttatt
     4441 aatcacttta ttattaagca ttataagctt ataatatttt atcaagggtt tataaacatt
     4501 ttataaaggg ttgatgatat gaaaagtgtg aaaaagttga gtgaagaatt aggggttagt
     4561 aagcaaacga tttttaataa tattaagcga ttgaatatag agacaataaa acaggaaaac
     4621 acttcgttta ttaaagaaga taccgatata gaaaaaatca ttcaaagagt aaatgaaaat
     4681 aaaaagaaat atggttttga aagtactact gaagacaaac aaaaaaaagg gtcggataat
     4741 attaattttg aatcgaaaag cgatgctcaa atagtagaaa tccttaaaaa tcagattgat
     4801 actttaaata atcaaattga aaaacaagaa agtcgtcatg aaacaacaat tgaattttac
     4861 agaaaagagt tgcaagaaag atcaaaattg cttgaaaatc agcaagttct ggcattagag
     4921 agtaataaaa agattcaaaa attagaaaat caattagaaa aagaaaaaca ccttaattat
     4981 ccctttgata catctactaa cactagacaa aacgttgatg cacaagaaaa aacatatact
     5041 actaatcctg taaatattaa cagagatcag gaagaaacta aagaaacaga gattcaatat
     5101 aaggatatat ctggaagtca aagcgacgaa agtacacaag gtgaagaagc aaaaaaagaa
     5161 gacgttccag ctaatctaaa tgacaatgat agtgatatag aagaaaagag tgaagaaacg
     5221 gaacctaaaa aggggttttg gagtcgtttg tttggtagct aaaataattg attaggcact
     5281 aaaagttata aaattttagt gtcttttata tagtgagaac ttctggtaat catcgattat
     5341 gtaaactaaa gtatttcaaa taattaactg tattaatact ctcatcatga ttgcgttata
     5401 aacgttcgtt attgagacaa aatttaatag tactactaca caaaaagaga gtgcgaattc
     5461 gcactctctt tttgtgataa atgaatggtt aagaaaataa ttcatccaca gttgtatcaa
     5521 acaatcttgc caacttaaat gctaatgtca atgtaggatc atatttattg ttttctattg
     5581 cattaatagt ttgcctagac acttttaatt ctttacttaa agtttcttga gaaattcctt
     5641 tttgttttct atattctcta attctatttt tcattaattt aacctttaga tgtatatttt
     5701 ttatttgtag tatagtaatt aaacagcata agaaatagtg ctacaattat aaagaaaatt
     5761 tctggatatt gagaaaacca agatccttca atacttggtg acaaacctgt aaatttaagc
     5821 actagtgtaa ataacataca atatacaatc caaataaaac ttttagccag tatttcaata
     5881 aacattttac gttgtctttc atcgtatcga tcttgttgtc cttttttatc taatgtttct
     5941 gaaggtaaat atttgttagt tatagccgtc cataaacaag aaaaaattaa aataataata
     6001 aaaggaatta aattcattat aacacctcat aaatgtaaaa agtatttgac atttatgagt
     6061 gtaaatattt ttgatacaaa tgtcaaatac tttttacatt ataatatatt ttaaaacacc
     6121 ttattagtaa aacaattact atatagaaag ctgcgaactc gtactctttt ttcttgtata
     6181 attattaaaa tctcaattca ataatatgtt atggaatttg agacactgtg agagacgctc
     6241 atttgcattg acaatcaatt tatcaagata atcccttatc atgtttaatt ctataaactg
     6301 tttgtcttgt gatattaact tctttcgcaa tcttactaat cgctttgcct tcttctaaca
     6361 tttccaccac gcgatgataa ataacacgtt tctgtgggtc tttagcattt ggtgaataaa
     6421 gtaaagggcg ccctttatat acgccttttt ccttcgcaac ttgaatccct tgcgcttgtc
     6481 gacgtttact ttcatttctc tcttgttctg aaaccattgc taatatttga atgattaaat
     6541 ctttcataaa cttatccagt aatggattgc caattacttc attcatcata ggtaagctgg
     6601 taatcattaa ctgaacgtct ttttctttta aataattcac agtttcaata atttcatcat
     6661 agttacgtcc taaacgatca atagattcca caacgaatcg atctcccatt ctgacaaaat
     6721 tgagtgcttc ttgaaataca ggtctatttt ccactgactt tcccgactgt ttctctgtaa
     6781 agattttctc cacaccaaac gtctttaaat tatccaattg tctttctaaa ttttgatcta
     6841 tcgatgatac tctcgcataa cctataatca ttcatcttca ctccataata aatatacacc
     6901 ctaatcatac gttttaattg ttataatttc aacaattctt tttgtatggt tagggtgtac
     6961 tcaaagtgta catatctata attaaaactt actaaaaata aatatagatt ttttctttgt
     7021 aacagaaccg ttcatttgaa ggtcatctct atcataaata tttaaattaa ctaaaaattt
     7081 taatcatctg tttaatactt atgaaatgta tttacggtag gtattaaaat tgatgaaatt
     7141 tatataagtg ctttcaatga aaatgaccgt ttatttatcc tcctgattta aggagttaga
     7201 aatttcgaaa acgattcttt ccatttgttg catttgttcc atgtccagat atttaataca
     7261 ttatagaaaa ccaaagacca tcactaatta aacgcagttt taaaatttca tcaggattgc
     7321 cttcgtcttt aaaatgggcc tcttttaata taagttctac tctttcatac ctaactcttt
     7381 caatgccatc gtttcatacg ccgattctcc tgattccata gcaaactcag cgataaacat
     7441 tgggaatact gcattgagtt ctattatatg ctctttcatt cgtggccata tttcacctcc
     7501 agctttaccg tatgctgtta taagttgctc taatccttcg tcgtcgaata cacgatggtg
     7561 tcctataaag tccattgatg ggtcggagtg ggttgcttca gtccagtcta tgagacctgt
     7621 gacgtttgct tggttatcta ccattatatg tcctggatgt aagtccccat gtatcatggt
     7681 cgcatgtcga ggccatagtt cgtcgttttc taaccattgt ttccatctgt tccataattc
     7741 atctgatacg ccataagttt ctttaacttt attcattctt ctttgaaagt cattttttat
     7801 ttcttgtatg gttttgatat ttatatgctg aacgttaatg ttttcttctg gtatgttgtg
     7861 taaatctacg agtgtttcag ctaatgtgtt aataaagttt tctggtaatg gtttgtgttc
     7921 aatttcccat acataatttt gtatttctgg atctattgtg gctgcgggtt tacctgtaag
     7981 ttttgggtaa gcaataaggt cttttgcatg tactttccac ttcggtattt caaatgaaac
     8041 attcttctgt aagaagtcta ccgtttgttt ttcgggtttt gttcgtttat aaacgtcagg
     8101 tcttcttggt agtctcaaaa cccattctac tccatgttta tcttttccaa atgcaacttg
     8161 aaaatcaagt cccgattcat tcaatgagat tgtttgaggt tggatgtgta attgatattt
     8221 ttctgcacat ttaataattt cattatgtcg agtcatttta ttggatcctt atttctatta
     8281 tatttttctt gtgaacgtat tttttatatt gatataaatg aacctaatat tcaatataaa
     8341 ttagtgtata gtttttaagt tatatcatga agagattgtc ctgttaattc ccatttttta
     8401 tctgatacat gtttaacaaa ccttgtatca tgtgatgtaa acaaaatgat tccaggatat
     8461 ttattcataa acatttctaa tgcttctaat gttttaatat ctaaaaaatt agttggttca
     8521 tccaaaatta acatattcgc tttcgttgaa aataatactg ctaacgataa tttcgttctt
     8581 tccccaccac tcaaaacatt acaagaacga tcaagtgctt catttaaacc caagttattt
     8641 aaaatagctc tactgaatga ttctgatgaa tccgtttcat ccattaaata ttgcaataat
     8701 gaaacgtcac gcatgtcttc ataagcaagt tgacgatagt atgccatttg cactttagga
     8761 gaacaatcaa ttccctctat ttggtggtaa atagcttcaa gtaaagttgt cttacctaca
     8821 ccatttgcac ctacgagcgc tatatttttg ccatatggta tttggaatcg tacttgtgtt
     8881 agcagttttt gacttccttt aaccaatgtt agattttgtg caatgattgg ataattatta
     8941 tggatatcat aaattttatt ttgtgggaaa ttgaattcat gataactttg tggtttttca
     9001 acttcttcca aatgttccat tcttttttca atatgcttag cttgtttttg agcagccttc
     9061 tcaaccgtgc ctttttgttt tgatgcactt aaacgatctg gtgctatact tttgtttttt
     9121 tgttttgatg atgcttgtgc catttgttgc gcttgatttc gtttagcttt actggcttgg
     9181 gacaatcttt gtttttcact tatatactgt tcatattgac gttgttgttc taactgttct
     9241 tgttcatatt gattttgata ctgtgtgtaa ttacctttaa acactcttat tgtgccatct
     9301 tcttgtatat cccaaattgt gtcagcaatt tggtctataa gtgctctatc atgacttact
     9361 ataattaaag taccatagta atattttaaa atatttttca gataatcttt accaatttta
     9421 tccaagtgat ttgtaggttc atctaaaagt aatatcggac tataatttga tatgacatta
     9481 gctaatttat attttgcttt ttcaccacca ctcatactgt cggttgtatg cataggtata
     9541 tggagttcac tcattaaact accgtctaac gtgttaaaat cattttccac atccatgtta
     9601 agctgttcaa aatattgaat ttcgccaacc gtttcaactg ttccagattc tggttttgtt
     9661 ttttgagcaa tcatatttag taatgtggtt ttgccagtac cattaccccc aataagtgct
     9721 attttttcaa attggtaagc gtaaagatga ccgatgttaa gtgatcgtaa atctgtcaat
     9781 ttatgattga tttggttaaa tttaattgta tattgttcca tatttataca ctccttgtat
     9841 atttaaagga cgtgtacgca aaaaaatgag acaggctact cccgtctcat tcacattaca
     9901 ctttcaaaca cttaaaaatt tattatgatg ttttagtatt tgaaagcgtt tattattcct
     9961 atcgaaggat aaaaaatgac ataagtcatc ctatgagtgt gtattgcgga caagcctttt
    10021 tgaattaatt tagttttgta ttcaaaaagg tttatttgag tctcatagaa gctgtcattt
    10081 taatcctcct tcatcgataa aatagtaatg ttaatataac attttgttgg tttttttaca
    10141 atatatctct aagtagaaag attatataac aaaagtataa aaatacaatt ttagattgat
    10201 tatacatctc cttaatttag agtgtactca taaaata
//
LOCUS       DAMQYT010000081        10235 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMQYT010000081 DAMQYT010000000
VERSION     DAMQYT010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10235
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.081"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..863
                     /locus_tag="PGA33_001533"
     CDS             <1..863
                     /locus_tag="PGA33_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6473341.1"
                     /translation="TFFLMKLMPGSPFNDAKLNAEQKEILNEKYGLNDPVATQYLHYL
                     KNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLTAMFIGVILGLILGVAAATKQNS
                     WVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWFPVAGWEGFSTAVLPSLALSAAV
                     LATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLFGHALRNALIPIITIIVPMLASI
                     LTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAITLLFSTLFIVSIFIVDILYGVID
                     PRIRVQGGKK"
     gene            863..1933
                     /locus_tag="PGA33_001534"
     CDS             863..1933
                     /locus_tag="PGA33_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6473342.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            1950..3032
                     /locus_tag="PGA33_001535"
     CDS             1950..3032
                     /locus_tag="PGA33_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6473343.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKIERVSFNEK"
     gene            3022..3963
                     /locus_tag="PGA33_001536"
     CDS             3022..3963
                     /locus_tag="PGA33_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDG6473344.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPAEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPNHFVFSTEEEAAQLRENKLVTQN"
     gene            3982..5637
                     /locus_tag="PGA33_001537"
     CDS             3982..5637
                     /locus_tag="PGA33_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDG6473345.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALN
                     DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            5849..7564
                     /locus_tag="PGA33_001538"
     CDS             5849..7564
                     /locus_tag="PGA33_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDG6473346.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKT
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVAN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     EKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
     gene            7615..8601
                     /locus_tag="PGA33_001539"
     CDS             7615..8601
                     /locus_tag="PGA33_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6473347.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSNGEVIFDGKRIDTLNEKQLPDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANKLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKLDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            8604..9584
                     /locus_tag="PGA33_001540"
     CDS             8604..9584
                     /locus_tag="PGA33_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDG6473348.1"
                     /translation="MENILEVNQIKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFNLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKHNNNRIILKGELPS
                     PSNPPQGCPFHTRCPIAQDMCKKSMPELKDIGNEHQVACFYVDKVGDLND"
     gene            9577..>10235
                     /locus_tag="PGA33_001541"
     CDS             9577..>10235
                     /locus_tag="PGA33_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6473349.1"
                     /translation="MIKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFTLGLLSTAGYIQYLRNDIIENSKKDY
                     V"
BASE COUNT         3805 a         1419 c         1908 g         3103 t
ORIGIN      
        1 ttacattttt cttaatgaaa ttaatgccag gttcgccatt taacgatgct aaattaaatg
       61 ctgaacaaaa agaaatttta aatgaaaaat atggattaaa tgatcctgta gctacgcagt
      121 atttacatta tttaaaaaat gttgttacag gcgattttgg taattcattc cagtatcata
      181 atcaacctgt gtgggatttg attaaaccga gactactacc ttcttttgaa atgggtctta
      241 cagcaatgtt catcggtgtg atactgggac ttattttagg tgttgcagca gctactaaac
      301 aaaattcttg ggttgactat acaactacag ttatttcagt tattgcagta tctgtaccat
      361 cttttgtact tgctgtactt ttacaatatg tatttgcagt taaattaaga tggttcccag
      421 tagctggatg ggaaggtttt tcgaccgcgg tattaccgtc acttgcatta tctgcagctg
      481 ttttagcaac tgtcgccaga tacataagag cagagatgat agaggtatta agttcagact
      541 atattttatt agcgagagct aaaggtaatt cgacaatgcg tgtacttttt ggacatgcac
      601 ttagaaatgc tttaattcca attattacaa ttatcgttcc catgttagca agtattttaa
      661 caggcacttt aacaattgaa aatatttttg gagttcctgg attaggggat caattcgtac
      721 gttcaattac aacaaatgat ttctcagtaa tcatggcaat cacactatta tttagcacac
      781 tgtttatcgt ttctattttt attgtagata ttttgtacgg tgtgatagat ccacgaattc
      841 gtgttcaagg aggtaaaaaa taatggctga aaataaaaac aatttgtcga ttaacgacga
      901 tcattctaat gcagctatga cgcatacctc tgacgctatc gcatcatctg attttattat
      961 tagagaatta gatttgaatc aggaacctga aatgcaacga gaaagcaaaa acttttggca
     1021 agatgcttgg gctcagttaa aacgaaataa gttagctgtt gtcggtatga taggtttaat
     1081 tatcattgta atatttgctt ttatcggtcc agttataaat aaacatgatt atgctgaaca
     1141 aaatgtagaa catagaaatc ttccggcaaa aatacctgta ttagacaaag ttccattttt
     1201 accttttgat ggtaaagatg cagatggcaa ggatgcttat aaagcagcaa atgctaaaga
     1261 aaattattgg tttggtactg atcagttggg tcgagattta tggacaagaa catggaaagg
     1321 tgctcaaatt tcattgttta tcggtgttgt tgcagcgatg ttagatattt ttattggtgt
     1381 tgtatatggt gcgatttctg gattcttcgg tggacgtgtc gatacgatta tgcaacgtat
     1441 acttgaagtc atagcatcta ttccgaattt aattgtcgta attttatttg tattaatttt
     1501 tgaaccatcc atttggacaa ttatattggc tatgtctatc acaggctggt taggcatgag
     1561 cagagttgta cgtggagaat ttttaaaatt aaaaaatcaa gagtttgtca tggcttcgaa
     1621 aacattgggg gcttcaaaat tcaaattgat atttaagcat attttaccta atacattagg
     1681 tgctatcgtg gttacatcaa tgtttacagt acctagtgct attttcttcg aagcattttt
     1741 aagtttcatt ggtataggtg tacccgcacc tcaaacatcg ttagggtcat tagtaaatga
     1801 tgggcgcgca atgttattaa tttatccaca tgaattattt ataccagcaa tgattttaag
     1861 tttattaatt ctattctttt acttatttag tgatggatta cgtgatgcat ttgatccgaa
     1921 aatgcgtaaa taaaaagggg gcatagcata tgactgaaag aatattagaa gtaaatgatt
     1981 tgcatgtttc ctttgatatt acagcagggg aagtgcaggc agtgagaggc gtagattttt
     2041 atttgaacaa aggggaaaca ttggcaattg ttggtgaatc aggttcaggt aaatctgtaa
     2101 caacaaaagc aattacaaaa ttattccaag gggacacagg aagaattaaa aagggagaaa
     2161 ttttattttt aggggaagat ttagcaaaaa aacctgaaaa tgagttgatt aaattacgtg
     2221 gcaaagatat ttcaatgatc tttcaagatc caatgacatc tttaaaccca acgatgcaaa
     2281 ttggtaaaca agtcatggaa ccattaatta agcacaaaaa ttatagtaaa gcacaagcta
     2341 aaaagcgcgc attggaaata ctaaatcttg taggtttacc aaatgcagaa aaaagattta
     2401 aagcatatcc tcatcaattt tcaggtggac aaaggcaaag aattgttatt gcaaccgcat
     2461 tagcttgtga acctaaagtg ctcattgctg atgaaccaac gactgcatta gacgtaacga
     2521 tgcaggcaca aattttagat ttaatgaaag aactacaaca aaaaatcgat acagcaatta
     2581 tttttataac gcatgattta ggggttgttg cgaatattgc tgatagagtg gcagttatgt
     2641 atggtggtca aatggttgaa acaggagatg ttaacgaaat attttatgat ccaaagcatc
     2701 catatacatg gggattatta tcgtcaatgc ctgatttatc aacaacaaat gacacaccat
     2761 tactagcgat tcctggagcg ccacctgatt tattacaccc acctaaaggt gatgcatttg
     2821 cgagacgtag tcaatatgca ttagatattg attttaaagt agaaccaccg tggtttaaag
     2881 tttcaccgac acattttgtg aaatcttggt tattagacgc acgtgcacca aaagttgaac
     2941 tacccgagct ggtaaaacaa cgtatgaaac cgatgcctaa taattatgaa aaaccactca
     3001 agatagaaag ggtgtcgttc aatgaaaaat gatgaagtgc tattatctat taaaaattta
     3061 aagcaatatt ttaacgcagg aaagaaaaac gaagtgagag cgattgaaaa tatttcgttt
     3121 gatatataca aaggggaaac attaggttta gtaggagaat cggggtgtgg taaatctaca
     3181 actggtaaat caattattaa acttaatgat attacaagtg gagaaatttt gtatgagggt
     3241 attgatatac aaaagattcg taaacgtaaa gatttgctta aatttaataa aaagatacag
     3301 atgatttttc aagacccata tgcgtcttta aatcctaggt taaaagtaat ggatatagta
     3361 gctgaaggta ttgatatcca tcatttagca actgataagc gtgaccgaaa aaaacgtgtc
     3421 tatgatttac ttgaaactgt tggattaagt aaagaacatg ccaatcgcta tcctcatgaa
     3481 ttttcaggtg gacaacgcca acgtattgga attgcccgtg cattagccgt tgaaccagaa
     3541 ttcattatcg cggacgaacc aatatcggca ttggatgttt caatccaagc tcaagtagtt
     3601 aatttattat taaaattaca acgtgaaaga gggattacgt tcctatttat agctcatgat
     3661 ctatcaatgg tgaagtatat ttcagatcgt attgcagtca tgcattttgg gaaaatagtt
     3721 gaaattggac cggcagaaga aatttatcaa aatccattac acgattatac taagtcttta
     3781 ttatcagcca ttccacaacc tgatcctgaa tcagaacgca gtcgcaaacg atttagttat
     3841 attgatgatg aagcaaataa tcatttaaga caattacatg aaattagacc gaatcacttt
     3901 gtctttagta ctgaagaaga agcggcacaa ctacgagaaa ataaattggt gacacaaaat
     3961 taaggggaag ggggaaatgc aatgacgaga aaatttagaa cacttatttt aattttgatt
     4021 gctacaattg cattaagtgg ttgtgctaat gacgatggta tttattcaga taaaggtcaa
     4081 gtattcagaa aaattttgtc atcagactta acatcccttg atacatcatt aataacggat
     4141 gaaatatctt ctgaagtgac tgcgcaaaca ttcgaaggtt tatacacatt aggaaaaggt
     4201 gacaaaccgg tgttaggtgt tgcgaaagct tttcctgaaa agagtaaaga tggtaaaact
     4261 ttaaaggtta aattaagaag cgatgctaaa tggagcaatg gtgacaaagt gactgcacaa
     4321 gactttgttt atgcttggag aaaaacagtt gaccctaaaa caggttctga atttgcatac
     4381 attatggggg acattaaaaa tgcgagtgat attagtactg gtaagaaacc tgtagagcaa
     4441 ttaggtatca aagcattaaa tgatgaaaca ttacaaattg aattagaaaa gccggttcca
     4501 tatattaatc aattattagc acttaatacc tttgcacctc aaaatgaaaa agttgccaaa
     4561 aaatatggta aaaattacgg tacggcagct gatagagcgg tatacaatgg tccatttaaa
     4621 gttgatgatt ggaaacaaga agataaaacc ttactatcta aaaatcagta ttattgggat
     4681 aaaaagaatg taaaattaga taaagtgaat tataaagtta ttaaagactt acaagccggt
     4741 gcatcattgt atgatactga atcagtagat gacgcagtta ttactgcaga tcaagtaaat
     4801 aaatataaag acaacaaagg attaaacttt gtgttaacga ctgggacatt ttttgtaaaa
     4861 atgaatgaaa aacaatatcc tgattttaaa aacaaaaatt taagattggc tatcgcacaa
     4921 gcaatagata aaaaaggata cgttgattca gtgaaaaaca atggctcaat tccttccgat
     4981 acactaacag ccaaaggaat tgcgaaagcg cctaatggca aagattatgc gagtaccatg
     5041 aattcgcctt taaaatataa tcctaaagaa gcaagagcgc actgggacaa agctaaaaaa
     5101 gagttaggta aaaatgaagt gacattttca atgaacacag aagatacacc agatgcaaaa
     5161 atatctgctg aatatatcaa atcgcaagtt gagaaaaatt taccaggagt tactttgaaa
     5221 attaagcaat taccgtttaa acaaagagta tcactagaac tgagtaacaa ttttgaagca
     5281 tcacttagtg gttggtctgc agattaccct gatcctatgg cttatttaga aacaatgacc
     5341 acaggtagcg cacaaaataa tacagactgg ggtaataaag aatatgatca attacttaaa
     5401 gtagcaagaa ccaaattggc acttcaaccg aacgaacgat atgaaaactt gaaaaaagca
     5461 gaagaaatgt tcctaggaga tgcaccggta gcaccaattt atcaaaaagg tgttgcacat
     5521 ttaacaaatc ctcaagtaaa aggattaatt taccataaat ttggtccaaa taactcactt
     5581 aaacatgtat atattgataa atcgatagat aaagaaacag gtaagaagaa aaaataatat
     5641 gctttgtaaa ttaggctgga gacatatctc cagtcttttt gtgttggata aaaactttgg
     5701 gaataaaaat ttaaaataag tcgtttttta aattactgaa attgattaaa tgcataaata
     5761 actgaatatt ctaaaaataa acttgtaata attttttcta tgagtaaact aaaaagaaaa
     5821 aattagattg aaagtaggag gcatatgtat ggggaagcta attaaatata tttcaatact
     5881 tcttattgtc gttttagtgt tgagtgcttg cggaaaaagc agtaataaag atgaaggagt
     5941 aaaagatgct actaaaacgg aaacctcaaa acataaaggt ggtaccttaa atgtagcatt
     6001 aacagcaccg ccaagtggtg tttattcttc gttattaaat agtacacatg cagattctgt
     6061 agttgaggga tattttaacg aaagcttatt agcaactgat aaaaaaatac gtcctaaggc
     6121 atatattgct tcatggaagg acatcgagcc ggctaagaaa atagaattta aaattaaaaa
     6181 aggtattaaa tggcatgatg gtaatgaatt gaaaattgat gattggattt attcaattga
     6241 agtcttagct aacaaggact acgaaggtgc ttattatcca agtgtagaaa atatccaagg
     6301 tgcgaaagat tatcatgaag gaaaaactga tcatattagc ggattgaaga aaatagatga
     6361 ctacactatg caggttacat ttgataaaaa acaagaaaat tacttaacag gatttattac
     6421 tggaccttta ttaagtaaaa aatatttatc agatgtacca attaaagatt tagcgaaatc
     6481 agataaaatc cgaaaatatc ctattggtat tggaccgtat aaagttaaga aaatcgttcc
     6541 aggtgaggct gttcaactcg ttaaatttga tgattattgg caaggtaagc ctgcactaga
     6601 caaaatcaat ttaaaagtta ttgatcaagc gcaaattatt aaggcaatgg aaaaaggcga
     6661 tattgatgtt gcgaatgatg ctaccggtgc aatggcaaaa gatgctaagt catctaatgc
     6721 tggtctcaag gtattatctg cgccaagctt agactacggt ttaataggat tcgtatctca
     6781 tgattacgat aaaaaagcta ataaaactgg taaagtgaga ccaaaatatg aagacaaaga
     6841 attacgtaaa gcaatgcttt atgcaattga tagagaaaaa tggatcaaag cgtttttcaa
     6901 tggttacgct agtgaaatca atagttttgt accatctatg cattggatag cagccaatcc
     6961 taaggaccta aatgattaca aatatgatcc tgaaaaagct aaaaaaatct tagataagtt
     7021 aggttataaa gatagagatg gtgacggatt tagagaagat cctaaaggta ataaatttga
     7081 gattaacttt aaacattatt caggttctaa tcctactttt gaaccaagaa ctgctgcgat
     7141 aaaagatttc tgggaaaaag ttggcttgaa aacaaatgtg aagttagtag aattcggtaa
     7201 atataatgaa gacttagcaa atgcatctaa agatatggaa gtgtacttca gatcatgggc
     7261 aggaggtaca gatccagatc catcagattt ataccacact gatagacctc aaaatgaaat
     7321 gagaacagtt ttaccaaaat cagatcaata tttagatgat gcattagact tcgaaaaagt
     7381 aggcattgat gaaaagaaac gtaaagatat ttatgttaaa tggcaaaaat atatgaatga
     7441 tgagttacct ggattaccaa tgttccaagg taaatcgata actattgtta acgataaagt
     7501 acgaaactta gacattgaaa ttggaactga tcaaagttta tataatttaa ctaaagaagc
     7561 ttagtaatgt aacctcgacg attttgtcga ggtttactta tatgggggga tgttatgaat
     7621 aatgtattgt tagaggttaa agatttagaa acatcattaa aaataaataa tgaatggtta
     7681 gcaactgttg aaaatatttc ttttgaatta tctaaaggag aagttttggg tatagtaggg
     7741 gaatctggtt gcggtaagtc catattaagt aagtcaatta ttaaattatt accagaaaag
     7801 atatctaaac taagtaatgg agaagttata tttgatggta aacgaatcga tacgctcaat
     7861 gagaagcaat tgccagatat tcgaggaaat gatattgcta tgatttttca agaacctatg
     7921 actgctttaa atcctgtatt taccataaaa aatcaacttg tggaatctat aaaatcacat
     7981 aaaaaaattt ctaaaaaaga agcaaataaa ttagcaaaag atttactaaa aaaagttgga
     8041 attgctagac aagatgaaat attaaatagc tatcctcatc aattatctgg tggtatgaga
     8101 caaagagtaa tgattgcaat ggccatttca tgttctccta aattattaat tgctgatgaa
     8161 cctacaacag cattggatgt cacgattcaa gcgcaaatat tagacttatt aaaagaattg
     8221 caaaaggaaa cgcaaatggc aattatgatg attacacatg atttgagtgt agttgctgag
     8281 ttttgcgata aagtcttagt tatgtatgca ggtcaaattg tagaatttgg aggcataaaa
     8341 gaaatactac acaatccgaa acatccttat acccaaaaat tattatcaac aattccaaaa
     8401 cttaaagaag agcagaaacg acttgaaacg atagaaggaa ttgtgccatc aatccaagca
     8461 tttcacgtta ataagtgcag atttgcaaat agatgtaaca aaaaactgga tatttgtaat
     8521 aatcaatctc ctaaaatgca tgtttgtgaa gatgtcattg tacgttgtca tttgtacaaa
     8581 aatgaatata aggagatata agaatggaaa atattttaga agtcaaccaa ataaaaaaat
     8641 actacaaaat taaaactgga ttattacaaa aaactcagta cgttaaagct gttgatgacg
     8701 tatcgttttc aataaaaaaa ggacaaactt ttggattagt aggagaatcg ggttgtggta
     8761 agtcaacgtt aggtaaagtg attatcaggc ttgaagatgc aacttcaggc tcaataattg
     8821 ttaatggtga agatataaca agattacaag gtaaaaaact cagaaaatca cgacaacaat
     8881 atcagatgat atttcaagat ccgtatgcat cattgaatcc gatgcaaatg gttggagata
     8941 tcatttcaga acctatttta aattataaaa aattgccaaa agaagaaata aaaaaagaag
     9001 tactatattt attaaaatgt gttggcctaa gtgaagatgc atattataaa tatgcacatg
     9061 aattttcagg tggacagaga caaagagtgg gaattgcaag agcattggct ttgcgtccga
     9121 gtttaattgt tgctgatgag cctgtaagtg cattagatgt atctgttcaa tctcaagtac
     9181 tgaatttatt aaaagattta caagaacaat ttaacttaag ctatttattt atcgcacatg
     9241 atttaagtgt agtaaaacat ataagtgatg tcattggagt tatgtattta ggtcatatag
     9301 ttgaaatcgc atctgataaa gaaatttatg aaaatcccaa acatccatat acaaaagcgt
     9361 tgatttcatc aataccacaa attgataaac ataataacaa tagaattata ttaaaaggag
     9421 aattaccttc gccaagtaat ccgccgcaag gttgtccttt tcatacaaga tgtccaattg
     9481 ctcaagatat gtgcaaaaaa agtatgccag aattaaagga cattggtaat gaacatcaag
     9541 ttgcttgttt ctacgtagat aaagtaggtg atttaaatga ttaaattaat acttaaaaga
     9601 ttagggttaa tgatcccgtt actaatttta atctcaatag ttgtattttc attagcaatc
     9661 atacaaccag gagatccatt ttcagatcta caaaacggta aaataaaaca agaagcaata
     9721 aatgcacaaa gggaaaaatt aggtctcaac gactcaatac cacatcaata cattagatgg
     9781 gtcaatcatg ttatacacgg tgatttaggt gagtcaatca aatataaaag gccggtaatt
     9841 gatgttattg aggaaagaat tccaaataca atattactcg gtgctatgtc attaattatt
     9901 acttatatta tctcatttgc tttaggaata acgtcaggta gatattctta cagtttgacg
     9961 gattatactg tgcaaatatt taattatttg atgttagcca ttccatcttt tattgcggga
    10021 gtatttgcaa tttttatttt ttcttttgaa ttacaatggt tcccgtttca aggttctgtt
    10081 gatattaacc ttaaagaagg tacttttgaa tattatatga gtaaaatata tcacacattt
    10141 ttacctgcat tcactttagg tttattatct actgctggtt atattcaata tttacgtaat
    10201 gatattattg aaaattctaa aaaagattat gtatt
//
LOCUS       DAMQYT010000082        10218 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMQYT010000082 DAMQYT010000000
VERSION     DAMQYT010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10218
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.082"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            79..1221
                     /locus_tag="PGA33_001542"
     CDS             79..1221
                     /locus_tag="PGA33_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDG6473350.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            1222..2340
                     /gene="mnmA"
                     /locus_tag="PGA33_001543"
     CDS             1222..2340
                     /gene="mnmA"
                     /locus_tag="PGA33_001543"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDG6473351.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            2802..3470
                     /locus_tag="PGA33_001544"
     CDS             2802..3470
                     /locus_tag="PGA33_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDG6473352.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEENPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFFNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            3472..5949
                     /locus_tag="PGA33_001545"
     CDS             3472..5949
                     /locus_tag="PGA33_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDG6473353.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSEEVMSDSTKTDTN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            6292..8922
                     /gene="alaS"
                     /locus_tag="PGA33_001546"
     CDS             6292..8922
                     /gene="alaS"
                     /locus_tag="PGA33_001546"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDG6473354.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAISGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            8985..9245
                     /locus_tag="PGA33_001547"
     CDS             8985..9245
                     /locus_tag="PGA33_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDG6473355.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            9249..9677
                     /gene="ruvX"
                     /locus_tag="PGA33_001548"
     CDS             9249..9677
                     /gene="ruvX"
                     /locus_tag="PGA33_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDG6473356.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            9692..10000
                     /locus_tag="PGA33_001549"
     CDS             9692..10000
                     /locus_tag="PGA33_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDG6473357.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
BASE COUNT         3743 a         1389 c         2017 g         3069 t
ORIGIN      
        1 gttaaacaga aaagtagaaa aatagtatgt aaggtgctat tattggaaca ttgataaaat
       61 tgtgaggagt gtccaaacat ggaaatttat gcagattatg ctgcgaccac accagtaaaa
      121 cctgaagtag tagatgcgat gatgacgatt tacaattcgc attatggtaa tccatcatca
      181 atacatgcta agggaagaga tgcgcgtaaa tatttagatg agtcaagacg tcaaattgca
      241 caattacttg gtgcagatac acatgaaatt atatttacga gtggcgcaac agaatcgaat
      301 aatacggcaa ttaaaggcat agtaaaagca aatgaacaat tgggtaatca cattatcaca
      361 tcgaagattg aacatcattc tgtgttacat gtttttgaac aattagagcg agaaggtttt
      421 gatgtaacat atctagatgt tgatgataca ggtgctattg atttagatca acttgaagaa
      481 acaattacag ataagacgat tttagtatct attatgtttg taaataatga agttggcaca
      541 gtgcaacaaa tttatgatat tcaagatatt attgctgaga caaatgcata tttccatgtt
      601 gatgcggttc aagcaattgg acatcttgat gttaagtttg acgaatttga gatcgacgca
      661 atgagtatta ctgctcataa gtttggcgga ccaaaaggtg tcggtgcatt actagttaaa
      721 gaccatgtta cactagatta tcctcaactt ggcggtgaac aagaattgaa acgccgtgca
      781 ggcactgaaa atcttgcgca aattgttggg atggctaaag cattacaatt agcagaaaag
      841 aatcgagacg ataacaatat tcatttaatg aatttaaaag aacaattttt agttaaatta
      901 caagaaagag cgattccatt tgaattaaat ggttcaatga cggatgctac aggccatatt
      961 gtaaatctat attttccgtt tgttgaagtt gaaacgatgt taacattgtt ggatatggca
     1021 caaatctatg tgtcgtctgg atctgcatgt acagcaggct ctacacaacc gtcgcatgtt
     1081 ttagatgcaa tgttcgaaga tgaggagcga tcaaatcatt cgattcgatt tagttttaac
     1141 gaattgacta ctgaaaatga aattaatgca attgtagctg aaattcataa aatatatttt
     1201 aaatttaagg aggagtcata attgtcaaat aaagatatac gtgttgtcgt tggtatgtca
     1261 ggcggtgtag atagttctgt aacagcccac gtcttaaaag aacaaggtta tgatgtcatt
     1321 ggcatattta tgaaaaactg ggatgacact gacgaaaatg gcgtatgtac tgcaactgaa
     1381 gattacaacg atgttattga agtgtgtaat caaattggca ttccgtatta cgctgttaat
     1441 tttgaaaaag aatattggga taaagtcttt acgtatttct tagatgaata caaaaaaggt
     1501 cgtactccaa atccagacgt tatgtgtaat aaagaaatta agtttaaagc ctttttagat
     1561 catgcgatga atttaggtgc agattatgta gcaacaggac attacgcacg catacatcgt
     1621 catgaagatg gtcatgttga aatgttacgt ggtgtagata ataataaaga tcaaacatac
     1681 ttcttgaatc aattatctca acaacaactt tcaaaagtga tgttcccaat tggcgacatc
     1741 gaaaagagtg aagtgcgtcg aattgctgaa gaacaaggac ttgttactgc taagaaaaaa
     1801 gattctacag gcatttgttt tatcggcgaa aaaaacttta aaacattttt atcacaatat
     1861 ttacctgcac aaccgggtga tatgataaca cttgatggta agaaaatggg taaacatagt
     1921 ggtttgatgt attacacaat aggacaaaga catggattag gtataggtgg agatggcgat
     1981 ccttggtttg ttgtcggtaa aaacctaaaa gataatgttt tatatgtaga acaaggattc
     2041 catcacgatg cattatacag tgattactta attgcttcag actattcatt tgtaaatcca
     2101 gaagataatg acttagatca aggttttgaa tgtacagcta aatttagata tcgccaaaaa
     2161 gatacgaaag tttttgtgaa acgtgaaaac gaccatgcac tacgtgttac ttttgctgag
     2221 ccagtaagag caatcacacc tggacaagca gttgtttttt atcaaggtga tgtgtgtctt
     2281 ggtggtgcaa caattgacga tgtgttcaaa aatgaaggtc aattaaatta tgttgtataa
     2341 acaatggcaa caataaatta cttatttgaa gtttcaacgt tgaaaatgac gaaagacagt
     2401 ttttgatgag aataattcat gaggatagag tctgggacat cacaatgtcc taggctctac
     2461 aatgttatat tggcgggacc acaacataga gaatttcgta aagaaattct acaggcaatg
     2521 ccagttgggg ataacgaatt taattttgtt aaaatatcat ttctgtccca ctccctatgc
     2581 atgaatctaa ttatgtattc ttatttttaa gtacataata gtggtggcta atgtggaaga
     2641 accattacat aataaaccgt taatggttct taagcatttc tattccattc ccgctttttc
     2701 atgaatgaag atgatattag attatatttt attcgttgtt aagtgattcg agacatacaa
     2761 tttatcaaga tgttataatt gatgagaaat gaggttcgta aatgatagat caacaaacaa
     2821 tttatcaata catacaaaat ggaaaaatag aagaagcgtt acaagcattg ttcggaaata
     2881 tcgaagaaaa tcctacaatt attgaaaatt atattaatgc tggtatcgta cttgctgatg
     2941 cgaatgagat tgaaaaggca gagcgttttt tccaaaaagc tttaacaata gatccgaaga
     3001 atggcgtcgt attttttaat ctagcaaatg tatattataa tcagcaacgt tatcaagaag
     3061 ctattaaatt atatcaacaa gcattacaaa cagagattga acaagttgat tgtaattata
     3121 tgatcggtat ggcgtttaat cagttagaat catttaagct ggcattgccg tatttaatga
     3181 ctgctgcgga actagataaa gacaaagatg cagaagttca atttcaatat ggtcttgtat
     3241 tatgtcaatt agaaatgttt aatgaagcca taactcaact taaacatgta ttaacgattg
     3301 ataaaaatca tgttgatgca agatacaatt tgggcttagc gttatttatg aaaaatgaag
     3361 atattgatga agcaataact cattttaaag aagctgtgac tatcgaccct aaacacttat
     3421 taagtcagca tgcgctgaaa acattcacta aaatgaaaga ggaggagtaa catgtcagac
     3481 cctacactat ttgattattc aatgatcaaa ggtactgttg aagcgatatt gtttcaaaac
     3541 agtgataatt tttatacagt gcttaaagta gatactattg aaacaaatga agactttgat
     3601 acaatgccaa ctgttgtagg gtttcttccg aatattgttg aaggcgatgt ctacacattt
     3661 aaagggcaag ttgttgatca tccacgttat ggtaaacaat taaaagcaga gacttttgaa
     3721 aaagaaatgc cacaaacgaa agaagcaatt ataagttatt tatctagtga tttgtttaaa
     3781 ggtgtaggta aaaagacagc tcaaaatatt gttaatacat taggtgacaa tgcgataaat
     3841 gatatattag atgaccattc agtgctagaa aaagtttcgg gattatctaa aaagaaacag
     3901 aaacaaattg cagaacaaat ttcagcaaat caagaatctg aaaaaataat gattcgttta
     3961 catgatttag ggtttggtcc taaactttca atggcaattt atcagtttta tctcggcgat
     4021 acactgacta ttttagatcg aaatccatat caattgattt atgatattaa aggtattggt
     4081 tttaataaag cagatcaact tgctagaaat attggtatcg cttataacga taatgaacga
     4141 ttaaaagctg cgttgctcta tacgttagaa gaagaatgta ttaaacaggg acatacatat
     4201 ttgcctatca atgttgttat agatttaacg gttgatgtat taaattatca agatgaagag
     4261 gtcatcgaac ctgaaaaatt agacgaaatg ttgcaatact taaatgagga aaaacgacta
     4321 attattgaca atgaacaagt tgcaattcca agtttgtatt attctgaaat caaaagtgtt
     4381 caaaacttat tcagaataaa gacgcataca aataaactta cagaaattga acagtcagat
     4441 ttacaaatgc atattggtga aatagaagat gcgaatcaag tgaactatgc agcttcacaa
     4501 aaagaagctt tgcaaacggc tatcaattct aaggtgatgc tattaaccgg tggaccggga
     4561 acaggaaaaa cgacagtaat taaaggtatt gttgaattat atgctgagat tcatggctta
     4621 tcattagatt atgatgatta tgtcaatgat gattatccag ttgtattagc agcgccaaca
     4681 ggaagagcgt ctaagagact acaagagtca actggattag aagcaatgac gattcatcgt
     4741 ttaataggtt ggaatcaaga cacaaagcca gaggatatat tagaaaatga aataaacgca
     4801 cgactcataa tcattgatga gatgtctatg gtagatactt ggttgttcca ccaattttta
     4861 agtgcagttc ctttagatgc acaattgata tttgtaggtg atgaggacca attgccttct
     4921 gtaggtcctg gtcaagtatt taaagattta attgaatcga aagctatacc acgagtaaat
     4981 ctgactgaag tatatcgtca acaagatggt tcaagcatta tagaattagc acatcgaatg
     5041 aagttgggtc agaaaattga tattacacag cgttttcatg atagaagttt tattaactgt
     5101 caggcaaacc aaataccaac agttgttgag aaggtagtta ctagtgctgt taataaaggg
     5161 tatactatgg ctgatattca agtgcttgca ccaatgtata aaggaaatgc cggtattaaa
     5221 cgtttaaatc aagttttaca agatatttta aaccctaaaa agaaagacac acgagaaatt
     5281 gagtttggtg atgtagtctt tagaaaaggt gacaaagtat tacaacttgt taataggcca
     5341 aatgataaca tattcaatgg tgacatagga gttattgttg gtattttttg ggcgaaagaa
     5401 aatgccctta ataaagatgt acttgttgtg gattttgaag gtaatgaaat tacatttacg
     5461 aaacaagata tgatggaatt aacacatgct tattgtacat caatccataa atcacaaggt
     5521 tcagaatttc ctattgttat catgcctatt gttaaacagt attttagaat gttacaaaga
     5581 ccgatattat atactggttt aactagagcg aaaacgagtt tagttttact tggtgatccg
     5641 gaagcatttg atattggatt gaaaacaaat ggtcaagcgc gtttaacaca attatgtaca
     5701 ttgttgaaaa attattttaa ttcagaagat gaagaaatgc tggaaaacac agcaactaat
     5761 gatacaggag cttcacaaac gacaatagat gatcaagtag aagcgccaat gtcatcgaat
     5821 gatagtgaag aagttatgtc tgattcaaca aaaacagata caaatgtatt aacggaagct
     5881 acaatgttta aaattgaccc aatgattaat atgggagaaa tcacgccgta tgactttata
     5941 gaacgttgac aacaagcgta aatgtaacta aaatagaatt aaattcgtat aaagatgagt
     6001 agttaatgat ttgtttatca gagatgtact ggttggtgaa aagtacaaca aacattagtg
     6061 aacgctactt tatgcacaac tgtatagtcg aattaaagtg ataaatgtgt tgaattgcat
     6121 ttataactag ggtggtaccg cgataacgtt cgtccctttt gaggatgggc gttttttatt
     6181 ttttaataat aaaaaatgat attgatttat actagttttg caaatcgact atctgtgtca
     6241 tcaattttta tggaatggta atttaaaaac agtaaatagg atgtgtaaat gatgaaaaag
     6301 ttaaaagcga gtgaaattag acaaaaatat ctagatttct ttgttgaaaa aggacatatg
     6361 gttgaacctt ctgcaccatt agtgccaatt gatgatgata cattattatg gattaattca
     6421 ggtgtagcaa cattaaagaa atattttgat ggacgtgaaa cacctaaaaa gccaagaatt
     6481 gtaaactctc aaaaagctat tcgtacaaat gatattgaaa atgttggttt cacagcgcgt
     6541 caccatacat tctttgaaat gttaggtaac ttctcaattg gtgattattt taaacaagaa
     6601 gcgattgaat ttgcttggga atttttaacg agtgataaat ggatgggtat ggagccagat
     6661 aaattgtacg ttacgattca tccggaagat atggaagcat acaacatttg gcataaagat
     6721 attgggcttg aagaaagtcg tattattcgc attgaaggta acttctggga tattggtgaa
     6781 gggccttcag gaccgaacac tgagattttc tatgatcgcg gagaagcata tggacaagac
     6841 gatccggcag aagaaatgta tccaggtgga gaaaatgaac gctatcttga agtatggaac
     6901 ttagtattta gtgaattcaa tcataataaa gatcatagtt acacaccatt acctaataaa
     6961 aatattgata ctggcatggg gcttgagcgt atggcctcag tttctcaaaa tgtacgtact
     7021 aactatgaaa cagatttatt tatgcctata atgaatgaaa tcgaaaaagt atcaggtaaa
     7081 caatatttag taaacaacga acaagatgtg gcatttaaag taattgctga ccacattcgt
     7141 acgattgcat ttgcaatttc tgatggtgca ttacctgcca atgaaggtag agggtatgta
     7201 ttacgtcgat tgttacgtcg tgccgttcgt tttagtcaaa cgttaggaat caatgagcca
     7261 tttatgtaca aacttgttga tattgttgca gacattatgg aaccatatta tccaaatgtt
     7321 aaggaaaaag cagatttcat taagcgtgtt ataaagtctg aagaagaacg attccatgaa
     7381 acattagaag atggtttagc gattttaaat gaattaatta aaaaagctaa agcgacaaca
     7441 aatgaaatta atgggaaaga tgcatttaaa ttgtatgata cgtatgggtt cccaattgaa
     7501 ttaactgaag aaatagcagt gcaagcagga ttgaaagttg atatgacaac attcgagtca
     7561 gaaatgcaac aacaacgtga tcgtgcacgt caagcacgtc aaaattctca atcaatgcaa
     7621 gttcaaagtg aagtattgaa aaatattaca tctgcaagta cttttgttgg ttatgatact
     7681 gcgacagctc aaacaacact aacacacttg atatacaatg gtgaagaagt ttcacaagtt
     7741 gaagcgggtg aaacagtata cttcatgtta acggaaacac cattttatgc aatcagtggt
     7801 ggacaagttg cggatacagg tattgtttat aatgacaatt ttgaaattgc tgttagtgaa
     7861 gtaaccaaag caccaaatgg tcaaaacttg cataaaggag tagtacaatt tggccaagta
     7921 aatgttggcg ctacagtgtc tgctgaagtg aaccaaaatg atcgacgtga cattcaaaag
     7981 aaccatagtg caacacattt attacatgca gcgttgaaat cagtactggg tgatcatgtt
     8041 aaccaagctg gttcactagt agaagcagat cgtttacgtt ttgatttctc tcattttggt
     8101 ccaatgacta atgatgaaat tgatcaagtt gaacgcttag taaatgaaga aatttggaaa
     8161 ggtattgacg ttaacattca agaaatggat attgcttcag ctaaagaaat gggcgcaatg
     8221 gcattattcg gtgaaaaata tggtgatgtt gtgcgtgtag taaatatggc accattttca
     8281 attgaattat gtggtggtat tcatgtccgc aatacttctg aaattggctt attcaaaata
     8341 gtaagtgagt caggtacagg agctggtgtg cgtcgtattg aagcattaac aggtaaagca
     8401 gctttcttat atttagaaga tattcaagag aaatttaata cgatgaaatc acagctgaaa
     8461 gtgaaatctg atgatcaagt agtcgataag ttaacacaat tacaagatga agaaaaagca
     8521 ttattaaaac aattagagca acgtgacaaa gaaatcacat cacttaaaat gggtaatatt
     8581 gaagatcaag ttgaagaaat caatggctat aaagtattgg ttactgaagt ggatgtacca
     8641 aatgcgaaag caattcgctc gacaatggac gattttaaat ctaaactaca agatacaatt
     8701 atcattcttg caagtaatgt tgatgataaa gtatcgatgg ttgcaactgt ccctaaatct
     8761 ttaacaaata acgttaaagc cggtgatctt atcaaacaaa tggcaccaat cgttggtggt
     8821 aaaggtggcg gtcgtccaga tatggctcaa ggtggcggta cacaacctga aaatatctca
     8881 aaatcattaa gctttattaa agattacatt aaaaatctat aatttaaagc cataatattg
     8941 tatgatgata gtgagttata tttgtaatta aaggagtgtc gcaaatggaa aactttgata
     9001 aaacaatgaa attcgactat gaagaacttc caacgcaaga tgtaagagat gttttaaata
     9061 atgtttatcg aacattagat gaacgaggat ataatgccgt aaaccaaatt gtaggttatt
     9121 tattatcagg tgaccctgcg tatattccac gccaaaatga agcaagaaat caaattcgtc
     9181 atattgatag agatgttatt atggaagagc ttgtttctta ctatttaaaa gagcaaaata
     9241 aataatttat gttacaacat aaaattttag gactcgatgt cggtagtaga acggtaggaa
     9301 ttgcaattag tgatataatg ggttggacgg cacaaggatt agacacactc cgaatcaatg
     9361 aagaaaataa tgaattaggt attgaccaat tagtagacat tattaaaaaa cataatgtgg
     9421 gtacagtcgt aataggacta cctaaaaaca tgaataattc aataggattt cgaggcgaag
     9481 cttcgttaac atataaagaa aaattattag aagcttatcc ttctattgag attgtgatgt
     9541 gggatgaaag attaagcaca atggctgctg aacgatcatt attagaagca gatgtttcaa
     9601 gacaaaaaag aaaacaagtg attgataaaa tggcagcggt atttatttta caaggctatt
     9661 tagattcact acattaaaag gagattttac tatgactgaa cataatcatg attcacaact
     9721 agaaattaat aacgaagaag aattattaac tttattcgat gaagagggaa atgaagtttt
     9781 ataccgaaaa gttttagaat tttatcatcc tgaattcaaa aaagagtatg ttatcttagc
     9841 tgaagaaggt gctcaatcag atgaagacga tatgattgag cttgtaccaa tgatcaatga
     9901 accagatgag tcaggtgacg gtggtaagtt agtaccaatc gaaactgatg aagaatggga
     9961 catgattgaa gaagttgtaa atactgaaat ggaagaataa ttaaaaagat tttactcttg
    10021 ctaaccttaa agatggggag gcaagagtat tttttaagtc aagcaatgta taaataatta
    10081 acgcttataa ttttattgaa aatatggtaa aatatctaaa gttaaatttt atatgaatga
    10141 ttcgcttcta taaataaaat agctttgcgt ctaacacgaa atcagtcatt taattggttg
    10201 gcaaaattat aatcaatt
//
LOCUS       DAMQYT010000083        10198 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMQYT010000083 DAMQYT010000000
VERSION     DAMQYT010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10198
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.083"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..78)
                     /locus_tag="PGA33_001550"
     CDS             complement(<1..78)
                     /locus_tag="PGA33_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473358.1"
                     /translation="MLGQYEKAISISNMIEIVFFSYIMTF"
     gene            complement(113..379)
                     /locus_tag="PGA33_001551"
     CDS             complement(113..379)
                     /locus_tag="PGA33_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDG6473359.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(379..630)
                     /locus_tag="PGA33_001552"
     CDS             complement(379..630)
                     /locus_tag="PGA33_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDG6473360.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSVMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(795..896)
                     /locus_tag="PGA33_001553"
     CDS             complement(795..896)
                     /locus_tag="PGA33_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473361.1"
                     /translation="MLRSVSDTNLLGQYEKAISISDMIEIVFLMYEV"
     gene            complement(903..1502)
                     /locus_tag="PGA33_001554"
     CDS             complement(903..1502)
                     /locus_tag="PGA33_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDG6473362.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVVYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(1521..1880)
                     /locus_tag="PGA33_001555"
     CDS             complement(1521..1880)
                     /locus_tag="PGA33_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HDG6473363.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDEVHYYAYDFSDKRVSYKQDFDSR
                     RYYQQHDADYHEENMTN"
     gene            2135..3385
                     /gene="fmhA"
                     /locus_tag="PGA33_001556"
     CDS             2135..3385
                     /gene="fmhA"
                     /locus_tag="PGA33_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HDG6473364.1"
                     /translation="MNFITLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKHNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
     gene            complement(3482..4213)
                     /locus_tag="PGA33_001557"
     CDS             complement(3482..4213)
                     /locus_tag="PGA33_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDG6473365.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     GAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(4210..4929)
                     /locus_tag="PGA33_001558"
     CDS             complement(4210..4929)
                     /locus_tag="PGA33_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDG6473366.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(4910..5689)
                     /locus_tag="PGA33_001559"
     CDS             complement(4910..5689)
                     /locus_tag="PGA33_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDG6473367.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(5810..7249)
                     /gene="mdeA"
                     /locus_tag="PGA33_001560"
     CDS             complement(5810..7249)
                     /gene="mdeA"
                     /locus_tag="PGA33_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HDG6473368.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVVSFFCSFMLQGKKKEVDSRQ"
     gene            7423..7527
                     /locus_tag="PGA33_001561"
     CDS             7423..7527
                     /locus_tag="PGA33_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDG6473369.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(7681..8367)
                     /locus_tag="PGA33_001562"
     CDS             complement(7681..8367)
                     /locus_tag="PGA33_001562"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDG6473370.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(8694..9560)
                     /locus_tag="PGA33_001563"
     CDS             complement(8694..9560)
                     /locus_tag="PGA33_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDG6473371.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
     gene            complement(10113..>10198)
                     /locus_tag="PGA33_001564"
     CDS             complement(10113..>10198)
                     /locus_tag="PGA33_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473372.1"
                     /translation="SVNYVKKRLIQNIQRILSKEVLYGEMQ"
BASE COUNT         3418 a         1879 c         1404 g         3497 t
ORIGIN      
        1 gaacgtcata atataagaaa aaaagacaat ctctatcata ttgctaatag aaattgcctt
       61 ttcatattgt cctaaaataa cttaccctat ttttatagtg tgacttttag atttaataat
      121 ggtatttaca tgatgctatt tttatatgat tgtcatctac tgtataaacc aatctatgtt
      181 cgtgattgat acgtctacta taatacccag tcagattcga ttttaacttt tcaggcttac
      241 ctataccttc caatgcacca tttctgtcaa tacttttaag taactcatta atcttcttca
      301 ccattttttt atcgttctgc tgaaaatact tataatcttc aaaggcttga ggcgaaaacg
      361 taatatttaa cctagccatt atacatctat atctttcgtt atagttttcc cacgttctag
      421 atctgcaatt gattgagcta agtgttcagc attatttggg ttctgttgga ggtaaagtgt
      481 ttccatcatg gagttataat ctgattctga catgattact acgtttttat catcagtaga
      541 tgttacagtt accataacac tatcatcatt tacttttgtc ataagtgcct ttaaattctg
      601 tcgagcgtat gaataatttt taataatcat atttatcgcc tccttttaac acacctcaat
      661 tgtacaaata tctgtacaat aaaacaagcg acacagaaaa aaactactaa tcaagatttc
      721 gcacggggaa gaatcaatac ctatcctgta ctgattccct actaaacaat gtttacatga
      781 taatttccac ctatttagac ttcatacatt aaaaagacaa tctctatcat atcgctaata
      841 gaaattgcct tttcgtattg tcctaataaa ttggtatcac ttactgatct caacaattaa
      901 gactatttga ttttatcttt taataacttc tcataacttt cataatcata tggatcttca
      961 actttctcgc cattaataaa tgcagttggt gtcgttttta tatgattatc tttcgctatt
     1021 tttttatctt tctctgcagc tttccaagac ttgctatctt ttgtcttgta atcttttata
     1081 attttatttt ccgtctcttt atctaaatgc agttgtttaa tatgtttatc taatagttct
     1141 tttgttaacc attctttatt ttcatcttgc tgggcagcaa ataattgctt ttgaaaatct
     1201 aaaaatgatt taggtgcata catcaatact gcatgactcg cacgcgaacc aacaattgag
     1261 tctttaccta agaaagctaa attgacaaat tggtattcca ctttgtgatt atctatataa
     1321 tttttacgca actttggcat gactttttca tctaattctt tacaataagg acatttatag
     1381 tcgccatata cgacaactaa tggtttgccg tttttcgaag atgtcgttgc tgattctttt
     1441 ttaccgcaag ctgttaaaat taacatgctc actataaata atgttagtaa ttttttagtc
     1501 ataataactc ctattcatat ctagttcgtc atattttctt catgataatc cgcatcatgt
     1561 tgttgataat atcgtctcga atcaaaatct tgcttatatg atacacgttt atcactaaaa
     1621 tcatatgcat aataatgcac ttcatcatta ctcaaaggtt tataagctaa aatgataatt
     1681 ttatccttat catagacata aatattagca tctttgcgtt caaaatgatc aacacgatca
     1741 ccgttgcctt ttttggcaag ttgttcttgt ttttgatttt gaattttaac tgcttcatct
     1801 atttctttca catatttatc attaccacat cctgatataa agactagtaa taataatacc
     1861 gtcgccacaa atcgcttcaa tgctcccact cctactatca acttccaact aactttcaat
     1921 ataattaata ctatacaccg tcaaacatta atgcgctaca actactacca atgcatttaa
     1981 tataaatatc caaagaaata acagttatgt atagtcaaag aaaaatatat ttcattttct
     2041 atgtgtattt gtaatcagtt tttgataaaa taaaaaaata gcattattaa taaattcaaa
     2101 aattatagtt caattcagag agggatggga taaaatgaac tttataacgt tgacttcgga
     2161 tgagttcaat gcgtttacaa caaagcattt ttcacattac acacaatcag ctattcatta
     2221 caatcataga gttgatttaa aaggcgatgt gcatcttgta ggggttaaag atgacaatgg
     2281 tcaagtgatt gcaggatgct tattgacaga agcacgcaca cttaaatttt tcaaatattt
     2341 ttatacacat cgcgggccag tgatggatta tacaaatcaa tcattagtag catttttctt
     2401 taaagcatta acgtcatatt taaagaaaca caattgttta tatgtccttg tagatccata
     2461 tttaattgaa aatttacgca atgcagacgg tgaaattgtt aaatcttatg ataaccgagc
     2521 atttgttaga acaatggata aattaggtta taaacaccaa ggtttccctg taggttatga
     2581 ttcaatgagc caaatccgtt ggctgtcagt gttagattta aaagataaga ctgaagacca
     2641 acttttaaaa gaaatggatt atcaaacgag acgtaatatt aaaaaaacat atgatattgg
     2701 tgtcaaaact aaaacgttaa cgattgatga aacgcaaact tttttcgact tattccatat
     2761 ggctgaggaa aagcacggtt tcaaattccg tgagttacca tactttgaag aaatgcaaaa
     2821 gttatacgat gaccacgcca tgttaaagtt ggcgtatatt gatttaaacg agtatttaaa
     2881 aacgttacaa ttaaagcaac aacaattaac agctgaactc tcaggtgttg aagaagcatt
     2941 ggaagaaagt cctaattcaa agaaaaataa aacgaaacgc acacagctag aacaacagct
     3001 aaatagcaat aagcgtaaaa tcgacaatac aatagaacaa attgaacaag atggtgcagt
     3061 cttaaactta gcttctgctt tatttatcta caatgagcat gaagtttatt acttatctag
     3121 tggttcaaac cctaaatata atgcttatat gggtgcctac catttgcagt gggaaatgat
     3181 taagtttgct aaagcgcatc atattgatcg ctataatttt tatggtatta ccggtgactt
     3241 ttcagagtca tcagaagact atggcgtcca acaattcaaa aaaggtttta acgcacatgt
     3301 agaagagtat atcggtgact ttattaagcc tatcaaacca ttactatata aggtacaaac
     3361 gtacttaaat cataaacgtc gttaaaatat cgctccaaga ttatgacaac ataacaaaga
     3421 accacgcatc atgtatatgg cttcatcgcc atactaaaac atgatacgtg gttctatttg
     3481 attattcttc atttataaca tttaagaaac gtcttaattc ttctgttttc ggacgattga
     3541 acatctcttc tggagcccct tgttctccga tcatgccttc atgaataaat acaatgttat
     3601 tagatacttc tttagcaaaa cgcatttcat gtgtcacaat gaccattgtc atgccttcat
     3661 tagccaaatc tttaataacc tttaaaacat cattcacaag ttcaggatca agtgcagatg
     3721 ttggttcatc aaacaacatc actttagggt tcattgctag tgctcttgca atagcaacac
     3781 gttgttgttg accacctgat aatgcatgtg gacgttgatc tttgacatgt gttaaaccaa
     3841 ctttctcaag taactcaagt gatttcccac gtgcctcatc ctttttcaac tttttaactg
     3901 tgataagacc ttccattaca ttttctaatg ccgtcttatg cggaaaaagg ttataacttt
     3961 gaaataccat accagactgt ttacgaactt ctatttgtga ttttttatct ttagatgtat
     4021 atgttttgcc gttaacataa actgtacctt cagttggaat ttctaatgca ttaatcatac
     4081 gtaacaatgt tgttttacct gaaccagatc gaccgattaa ggttacaacc tcaccttgtt
     4141 caacagataa atcaatacct ttgatgactt caacatcatt aaatgattta tggatattgt
     4201 tcaattgaat catgagcgat accctctttc aatataagat tcatagatac cttggataat
     4261 tgaaataata aagcatacca cccagtacat tagcgcaact aacacataaa ttgttaaata
     4321 ttcatatgtt gtagacgcaa cttcttgagc ttttctaaac atttcagcca ctaaaataaa
     4381 tcctaataat gatgtatctt tgattaaact taaaaatgta ttacctagtg caggaatcga
     4441 cactcgaatt gcctgcggta aaatgatacg ttgtatcgtt tgaccatacg tcataccgat
     4501 ggaatatgca gcttctgttt gtcctttcgg tatagaaata ataccgccac gaataatttc
     4561 cgaagcatac gcacctacat ttaatgataa accaataata gcagccacta caggtgccaa
     4621 tgtccattgg ttgtcagcgt catttgttaa taatctacct aattctggaa taccataaaa
     4681 tatgataaat agttgtacta tcattggtgt tcctcgaata atagatacat agacacgtga
     4741 aatacttctt aaaattttac tcgttgaaat tcgcattaat gctgtaaata atgcaataat
     4801 caatcctaaa acaaatgtaa ctaatgtaat aggaattgaa tatttgacca aaccttctag
     4861 cataggtccg aaagcttgtt ttgcagcatc tagcgcatgt tgttgttcgc tatttagatt
     4921 tagaaacatc ttgaccaaac catttcttac ctattttagc taattcaccg ttttcctcga
     4981 tttttttcaa gccatcattg aatttttgaa ctgtttcatc atctgctttt ttagaaaatg
     5041 caaatgcaga tctactttgt tcagcattac ctttgattgc tttgatctta gcattaggtt
     5101 tttgtttttt ataatccaag tatgacagac tatcattaaa tgtaccatca acacgcttag
     5161 acaataataa atccattgat tggttaaagc catcaacttt tgtaatatca gcacctttat
     5221 ccttagctaa tttaccataa ttagatgtga atgtttgtgc taacttctta cctttaacat
     5281 catcaaaatc tttaatatct ttttcatttt cacgaataac taaaacagca cttgagaatg
     5341 tgtaaggctt agaaaattta tatttctttt ctctatcagg attaatacct acttggttcg
     5401 cgattacatc aaaacgccct gcgtctaaac ctgcaaacat agaatcccaa gaagtttcat
     5461 taaatttaag ttttaaacct tcttctttag ccactgcttt aataacatca atatcgtaac
     5521 cagttaattt accatcttta tcgtggaaac taaatggtgc ataagtcccc tcagtcccaa
     5581 cattaattgt cttgctatct ttactagctt ccttgtcttt cgacgaattg tttccgcatg
     5641 cagccaatat gaaaacgaaa gctatcatca caaataaaag tcttttcata gaatatatct
     5701 ccttattctt attattctaa tcggaataaa aatattttaa tctgaaatgc cggataagtc
     5761 aatatgttga acttttgcta aaaagcttta tatagctagc aacttatatt tattgacgag
     5821 aatcaacttc ttttttctta ccttgtaaca taaatgaaca aaagaaacta acaactgtaa
     5881 tcaataatgc aaataaaaat ccagcgtgat aaccatgcaa catcgtatca actttaattt
     5941 gattcatcat atcaattttg ttcatgccat tatagtctga catagttgga gaaaagtttt
     6001 tgcttgcatg ggataaaata gtaattaatg cagctgttcc gatagaacca gcaatttgtt
     6061 gtactgtgtt tgtcatagat gaaccatgtg cattcatttc tggctttaat tgattcatcg
     6121 tatgggtcat tactggaatt aatcctaacg cgataccaac cattcgaatc gcataaactg
     6181 ttgccaacat gattgttgat gtttgttcat ccattacaac aaaataagcc gtagtaataa
     6241 caacaatcgc catacctact aaagcaagat ttctaggacc aaacttttca tataaagcac
     6301 ccgaagtcat cgacataaat gccataacaa ttgctcctgg taataaagtt aatccagaat
     6361 ctaaagctga acgatgcaat aaattttgca caaagatagg taaaatcgtt tccgaaccaa
     6421 tcatcgatac cattgtgaca cccataataa tgataccaac tgtaaattgt ttatttttaa
     6481 atacagcaaa atttaataat ggtgttgata gccgatattg acgtaataca aataatgcga
     6541 ctaaaacaac gcctccaatc atagatacta atacgatagg attatcaaaa cctttttctg
     6601 aaattgaact cgttccatac aataacccgc cgaaacctag tacagaaaaa atgacagaaa
     6661 taaaatcaat aggtacttta attgtagttc caacattttt tatcgtttta aatccaatta
     6721 aaaatgtcac agctgcaatt ggagcgacaa catggaaaag cgatctccag ttaaaatatt
     6781 caacaaggta acctgaaaga gtaggtccaa tagctggtgc taaaccaatt accaaaccaa
     6841 atatccccat atatttaccg cgttcatgtg gttggaaaac atccaaaatc gttgtcatca
     6901 tcaatggcat cataatacct gcgcctaacg cctgaataat tctagcaact aataatgttg
     6961 caaagtgcgg actcaagccg ccgagtaatg tacctaagaa aaagatagaa atacccacta
     7021 aaaacacttg tcttgttgta taacgttgta taacaagcgc ggacaaagga ataacaatcc
     7081 cattagtcaa taaaaaagct gttgtcagcc attgaacttg tgtatatgaa atttcaaaat
     7141 ctttcataat acttggtaaa gccgtcacta atagtgtttc atttaataat ccaaaaaatc
     7201 cacccatcag catagtaata atcatcgtta atttttgttt cttcgacatt taaagcttcc
     7261 ccctcaccat ctaaaaaacg acacacattt ttagatatcc ggtaattata cccattttcc
     7321 aaaaaattag cacgacttta ttacaatttc tttaaaacgt aatcattact atttacatat
     7381 gagtagttat cggtcataat atatttacaa ggagggatag agatgaaatt agatttacag
     7441 actgctcgtc gtaatttaaa tagccctaac attaaaacaa gaaagcgtgc tttaaagatt
     7501 attaagcaac ataaaagagc gaaataattg ttttaatttg actatattgt atgtctaact
     7561 atattttcga gtatccctag ctcgttaata taaactctat acttaactat ttccttacat
     7621 tacttcacat atttaagagc tgcatataca ctcactcctt gtgtacatgc agctcttttt
     7681 ttataagtag tatttatcta taacttctaa atcatccgtt aattcataaa caagcggtgc
     7741 acctgtttta atttcataat taatgatatc ttcatctgac acatcttcaa gatatttaat
     7801 caatgcgcga attgaatttc cgtgtgcaga aactaatacc gtttgaccat ctagcaaata
     7861 ttgtgaaata tgatctgtcc aaaatggtat cactcgaact aaagtatctt tcagactttc
     7921 agaataaggc atcatacgtt tatctaaatg attatatcga cgatcagcta agtaagcttc
     7981 acgttgttct tcggtttcag caggtggttt cacatcataa gaacgacgcc aaatatgtac
     8041 ttgttcttct ccaaattctt ttctagcatc atctttattt aagccttgca atccaccata
     8101 gtggcgttca tttaaacgcc agcttttata tacaggaatc cattgttgtt tagattcagt
     8161 taaaatataa tgcgttgtat ctaaagcacg tgttaataac gatgtaaaag ctacatcgat
     8221 ggcaatgtta ttttctctta ctttttcacc tgctctagtc gcttcattaa taccttgttc
     8281 agataaatta acatcttccc atccagtaaa taagttttta gcattccact cgctttgtcc
     8341 atgacgacat aaaattaatt ttggcataac ttataacctc tttcctttaa tctttaggac
     8401 gttatttcag ggatatttta agatgattta atcttagtaa ttttcttttc aatatatttt
     8461 aaaattcaat gattaaatca attatcacta gacattaaat acataaatcc tattccccat
     8521 tttcattttt taattcataa atgaatcaat aaccacctaa taacaaatca tattatacac
     8581 ctttgttcgc tatttttcta aggtttaaaa aatattttta ggtaaaccta aaaatagatg
     8641 taataaaaac gcctcctcag atatttatat atctatgaag acgtttaaat acattataga
     8701 tggtctggtt ctgggtgaac gtatactgag gaaatacctt ttttgtgcaa atgatgttcg
     8761 acattgtcac aaatttgatg cgcttctact aaggaaaggt tagcatctac aacaattgtg
     8821 acatcaataa acacactact tccatggtaa cgccctttaa tacttttaac ttcttgtact
     8881 tcatcaactt ctaaaatatc attgcgatac gcttctaatt cagtttcatt gaaaccatca
     8941 ctcaacataa aaattgcttc tttaaaaata ccaaaaccag tataaacgat tagtaagcct
     9001 agtaatgttg ctaaaataat atcgacaatt gggaaaccga tttgcgtaaa aattaatcct
     9061 atcgctgttc caatgctgac taaactatcc gataaattat ctttggcagc cgaatttaaa
     9121 gaactacttt tcgttctttt cgctagtctt tgattgactg caaatacaat caacattaca
     9181 agaccactga ttaagctgac gataattgtt attgcgttag gtacaacgtc atcttctttg
     9241 aacaaacgag gtgcattttg aataactact tggataccta caaacataat gacaaatgac
     9301 accaataatg aagaaatatt ttcagacttc aaatggccat aaggatgatt tcgatcggca
     9361 ggtttaattg aaattttcaa tccaataata acagctaaag aaacgataat atctgtcata
     9421 ttgtttaatg catcggctct tacagctgca gagttaaaga caaaacccgt gacatactta
     9481 acaatagata agattatata tacaattaaa ctcaaataag caccgcgttg cgccaattta
     9541 agattttcat tatgagacat gcgttgaacc accttgaatt agtatagtaa caatattatg
     9601 aatgattcat tttaatttta caacgttttt aatttttata aatttttata aaattaaact
     9661 aatttattca ttcgcaaccc ctaaaaataa tttttagcct ttctgcgaat tttatgagct
     9721 agaaaggcgc ccaactctcc ctgtttgtta actttcgcct cgaaagtttc tatgttggga
     9781 ccctatgcat tagtagcgaa gctatctaat gtgttccttt attttctgcg aattttatga
     9841 gctagaaagg cttatgcagt tgacgtttta cgtccaactg cgttcctccg tcttcttcaa
     9901 atttatttgt tagaaaggca cccaactctc cctgtttgtt aactttcgcc tcgaaagttt
     9961 ctatgttaga accctatgca tgagttgcga agctatctaa tgtcgtgaac taattatata
    10021 gaagaaaaag tgcatcaatg acaaattaaa tgagatttct actctaccaa actctcttcg
    10081 aaagacaatt ttctcctcta tttattagca aactattgca tttctccata tagtacttcc
    10141 ttacttaaaa tacgctgaat gttctgaatt aaacgctttt ttacatagtt aacactag
//
LOCUS       DAMQYT010000084        10103 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMQYT010000084 DAMQYT010000000
VERSION     DAMQYT010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10103
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.084"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(50..3223)
                     /gene="carB"
                     /locus_tag="PGA33_001565"
     CDS             complement(50..3223)
                     /gene="carB"
                     /locus_tag="PGA33_001565"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDG6473373.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            complement(3216..4316)
                     /gene="carA"
                     /locus_tag="PGA33_001566"
     CDS             complement(3216..4316)
                     /gene="carA"
                     /locus_tag="PGA33_001566"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDG6473374.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            complement(4318..5592)
                     /locus_tag="PGA33_001567"
     CDS             complement(4318..5592)
                     /locus_tag="PGA33_001567"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDG6473375.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            complement(5610..6491)
                     /locus_tag="PGA33_001568"
     CDS             complement(5610..6491)
                     /locus_tag="PGA33_001568"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDG6473376.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            complement(6519..7826)
                     /locus_tag="PGA33_001569"
     CDS             complement(6519..7826)
                     /locus_tag="PGA33_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDG6473377.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            complement(8044..8571)
                     /gene="pyrR"
                     /locus_tag="PGA33_001570"
     CDS             complement(8044..8571)
                     /gene="pyrR"
                     /locus_tag="PGA33_001570"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDG6473378.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            complement(8970..9887)
                     /locus_tag="PGA33_001571"
     CDS             complement(8970..9887)
                     /locus_tag="PGA33_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDG6473379.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNNDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTSEYIERHAELPQDFEDLLDTIRKRDA"
     gene            complement(9887..>10103)
                     /gene="lspA"
                     /locus_tag="PGA33_001572"
     CDS             complement(9887..>10103)
                     /gene="lspA"
                     /locus_tag="PGA33_001572"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDG6473380.1"
                     /translation="QVAISLLFAGALGNFIDRILTGEVVDFIDTNIFGYDFPIFNIAD
                     SSLTIGVILIIIALLKDTSNKKEKEVK"
BASE COUNT         3057 a         1991 c         1492 g         3563 t
ORIGIN      
        1 tctttaagta aatggttaat tacagcccca ccgatacagt ttgattgatt tacatttgac
       61 gcattgtaaa tgtcatactt tcaattacat tcgttaaggc attagctgta tctaaagatg
      121 tcaaacatgg aataccattt tcaactgtag tacgtctaat ttggaagcca tccctttcta
      181 cttctttacc tttagtcatt gtatttataa cgatttgaac atcaccattt tgaatacgtg
      241 ttaataaatc attttcgcca ccaattttgc ctactacttc tgcaggtatg tcatactcag
      301 ctaatttatt agctgttcca gacgttgcta aaattttata gccaacttca tttaagcgtt
      361 gtgccaattt aacaacttcc tctttatctt tgtcactgac ggtcattaat actgtaccgt
      421 gatctttaac ttcaacgcca ctacctgtta accctttgaa taacgccttt tctaatgtag
      481 tatctttccc catcacttca cctgttgact tcatttcagg tcctaaagta atatcaacat
      541 ttttcaattt attaaaacta aatactggtg ctttcacaaa gacaccctca gcatatggtt
      601 gaaccccttc ttgataaccc atatctgtta gtttttcccc aatgattgct cgcatagcta
      661 attgtgccat tggaatatca gtaattttac ttaagaatgg taccgttcta ctagaacgtg
      721 gatttacttc taaaacatac acaccatcgt gagctataac gaattgaatg ttgattaagc
      781 caatgatgtt taaaccttta gctaatttta tagtatagtc ctcaagtgtt gctaactcgt
      841 cttctgtcaa agtttgtggt ggatatacag cgattgagtc accactatgc acaccagcac
      901 gttcaatatg ttccatgatt cctggaataa tgaccgtttc tccatcacaa atcgcatcaa
      961 cttcaatttc tttaccagtt aaatatctat cgactagtac cggatgttcc ggactcgctt
     1021 ttacagcctg ggtcatatag ttttctaact ctttgtcatt gtctacaatt tccattgcgc
     1081 gaccacctaa tacataagaa ggtcttacta caaccggata tccgatttct gcagcattcg
     1141 ctaatgcttc ctcaggtgat gtagctgttt tcccttgtgg ctgtggcacg ttaatttttc
     1201 ttaatagtgc ttcaaattct tttctatctt cagcacgatt tagattttct agtgaagtac
     1261 caagtatttt aacaccatgt ttagccaatt tgtctgctaa attaatcgct gtttgtcctc
     1321 caaattgtac aacgacacct ttaggttttt ctaaattaat gatattcatc acatcttctt
     1381 cagttaaagg ttcaaagtat aatttgtcag aaattgagaa gtctgttgaa actgtttctg
     1441 gattgttatt cacaattatc gcttcgtacc ctgctttttg aattgcccaa acggcgtgaa
     1501 ctgtcgcata gtcaaattct acaccttggc cgattcgaat tggtccagag cctaatacta
     1561 agattttttc tttgtcagta actatggatt cattttcagt ttcgtatgta ccataataat
     1621 atggtgttga agattcaaat tcagctgcgc aagtatcaac catcttgtaa acaggtttaa
     1681 tatcattttc catacgcaat tgatatactt cttcttccgt catattaaag cgatgcgcta
     1741 ttgttttatc actaaatcca taatcttttg catatttaag atattctaaa tcaccttgat
     1801 gctcttttaa ttgatgctca atatcaataa tgttttggaa cttgtgtaag aagaagtaat
     1861 caatctgagt catattatga atttcttcta atgttgtgcc tcttctaatt gcttcgccga
     1921 tgaaaaataa tcgttcatca tcttggtgtg aaatacgttc tttaatataa tcaagatcga
     1981 agctttcacc atttggtaat cctaagtgat gcacaccata ctcaagtgat cgaattgctt
     2041 ttaacaatga ttcttcgtaa gttcgaccaa tggccataac ttcacctgtt gctttcattt
     2101 gtgtgccaag ctctcgttct cctttttcaa atttatcaaa aggaaatctt ggtatttttg
     2161 aaatcacata gtctaaagtt ggttcaaacg ctgcataaga tgttcctgta attggattta
     2221 acatttcatc taatgttaga ccaaccgcga ttttagcagc taattttgca ataggatatc
     2281 ctgttgcttt tgaagctaac gctgatgaac gtgatacacg cggatttact tctataatat
     2341 aataatcgaa tgaatgggga tctaatgcta attgaacatt acaaccacct tcgataccta
     2401 aagctcgaat aacttttaat gaaacatcac gtaacatttg atactcaaca tctgataatg
     2461 tttgactagg agccacaaca attgaatcgc ctgtatgaat accaactgga tcaatatttt
     2521 ccatgttaca tacaacgatg gcattatcgt ttttatcacg cattacttcg tattcgattt
     2581 ctttaaaacc tgcgatagat ttttctaata aacattgcgt tgctggacta taatgaagac
     2641 catttgagac gatttcatgt aattcttcat cattatgaca aataccgcct ccggtaccac
     2701 ccatcgtaaa tgccggtcta acaattagcg ggtatcccac ttgctctttg aatttaaagg
     2761 cttgctctac tgtatttaca atgtcactct ctggtacagg aacgtttaaa tcattcatta
     2821 atgttctaaa catttcacgg tcttctgctt gttgaattga tgttagctca gttcctaata
     2881 attggacgtt attatcttga agcacaccac tttcgtgtag ttgaatcgcc atgtttaaac
     2941 ctgtttgacc acctaaagtt ggaagtaaag cgtcaggttg ctctttacgt ataattcgcg
     3001 ctataaaatc atgagttaac ggttcgatat atactttatc cgcaatttcc ttatcagtca
     3061 tgattgtcgc tggatttgaa tttacaagaa taactcgata tccctcttct tttaaagcta
     3121 gacatgcttg tgttccagca taatcaaatt cagctgcttg acctatgata attggcccag
     3181 accctattac taaaattgtt ttgatatcat tacgtttagg cattgatatg acgctccttt
     3241 tccttaaaat tagtcatcat tgctacaaaa tcatcaaata gatagtttga atctgacggt
     3301 cctggatttg cttcaggatg gtattgaaca gaaaatgctg gtaatgtttt atgttttaaa
     3361 ccttctacag taccatcatt taatgcaaga tgagtaactt ctaaatcagt actttttaac
     3421 gaatctatat ctattgcata tccatggttt tgactcgtaa tatcaacttt tccagtctct
     3481 aaatttttaa ctggatggtt cgcaccacga tgaccaaact tcattttaaa tgagcttgct
     3541 ccttgagata atgcaaaaag ttgatgtcct agacagatac caaagaacgg aattttccct
     3601 aaaattcctt gaatcattgg aatcgcacat tctacaactt caggattacc tggtccgttt
     3661 gatagcataa cgccatctgg agccattgct aaaatttctt cggcagtagt tgtatatggt
     3721 acaactgtga cgttacaacc tctgacgttt aattctcgaa caatattttg cttcttacca
     3781 aagtctacaa gtacgacact tagatcctta cctgtcgaaa catacggtgt tttagtcgaa
     3841 acgatttcta cttcgttttt aggtaattct acttgttgta aatgtttgac aagttgatca
     3901 atatcttctt ttcgatcagt aaaaccagct tttaacacac cgtgttgtcg aattttacgc
     3961 gtaatacttc ttgtatcaac acctgcaatc cctggaattt gatgcaattc taaaacgtca
     4021 tgaagtgtct tttgctgtct aaaattactt ggatgcgcac tcgcttcttt cactacaata
     4081 ccgtttaatg taggtactaa tgattcaaaa tcgtctctat tgataccata attaccgatt
     4141 aatggatacg taaaagtaat gatctgacct gtatatgatg gatctgaaat agtttcttga
     4201 taacctgtca tcgctgtatt aaatacaatt tctcctacag ttaagttatc agatcctaaa
     4261 cggtagcctt cgtaaaaaga accgtcttct aacactagat aacgtttgct ttgcatatta
     4321 tttatccccc tcaaatttaa cttcgccttc aaccattgtt aagatcggat ttccataaac
     4381 tttatagccg ataaatggtg tattatctgc ttttgataag aaatcttctc ctttaatttc
     4441 ttgttcacta tctaaatcaa tgattgttaa atctgcataa ccattttctt ttaatgtgcc
     4501 gtattctaaa ttaaatgtct cacatggttt aattgttaag taatctacta attgttgtaa
     4561 tgtccaatca ccatttttta caaaatgcgt atataataat gggaatgctg tttcactacc
     4621 aacaattccg aatggtgctt tttccattgg ttgtgctttt tcatcacgtg catgtggtgc
     4681 atggtctgtt gcgatacagt caattgtacc gtctagtaac ccttctaaca aagcctctct
     4741 atcttcagta cttctcaatg gtggattcat tttataaatg gcattattac caggaatatc
     4801 atcttctgtt aacaataaat ggtgtggtgt aacttcagct gtaacatgaa tgcctgcgcg
     4861 tttagcgtca cgaatgactc taacactttc tttagtagaa acatgacata catgataatg
     4921 acaacctgct gcttcagcta atagtacatc tcttgcgatt tgaacagatt cacaaatgtt
     4981 tggaatacct ggtataccta actctttact gcgtttccct tcatgcattg caccaccata
     5041 gattaatgaa ttatcttcac agtgtgctac gatggctttg tttacttttg cagcttcaat
     5101 catgccttca tacatcatgc ttgcagtttg tactcctaca ccgtcatctg taaacgcaaa
     5161 ggcaccttct tttactagtg ctgggaaatc aaccaattct ttacctaatt gacgtgttgt
     5221 aattgaagca taaggtaata cacgtacttg agcattgtca tcgattaatt tttgtaaagc
     5281 ttcaaaatgt tctacagaat ctggtaccgg tcttgtgtta ggcattggac atacagttgt
     5341 aaatccgcct ctagcagcag ctttagtacc agtttcaatt gtctctttat attcaccacc
     5401 aggttcacgt aaatgaacat gaacatcgac aaatccaggt gacacaaagt gacctttcgc
     5461 atctatgatg tcaacaccat tgcttggttc aattgcaggt gcaatttgtt taattacctt
     5521 accatcaatt aaaatatctg cttgttgtaa ttcgccattt tgtaatactt taccgttttt
     5581 aattaatttc atcattttcg tccccttacc tattttaata attcatcaat gactgccatt
     5641 cttaagtaaa cgccattttc catttgctta aaaattcttg attttgaagc ttctactaaa
     5701 tcgctttgta tttctactcc tctattcaca ggtgccggat gcataacaat agcatgttct
     5761 tgtaatttgt tatagcgtac ttcatttaag ccatgctttt gatgataatc atctgctgca
     5821 aatctagttt cttctgcaag cccatgcctt tcatgttgaa ttcttaataa cataactata
     5881 tctactgttt ctataacatc atctatattt acataaggtg cttctaaaga atcatcaatc
     5941 caagcatttg ggctattaaa cattacgttt gcacctaatg cttttaaact atggtaatta
     6001 ctacgtgcga cacgtgaatt tttaatgtct ccacaaatca atacattcaa gccttcaaaa
     6061 tatccatatt cttcatatat cgtcattaaa tcaagtaaac tttgtgttgg atgttgtcca
     6121 ctaccatcac cagcattcgc aattgggatg ttaatattcg ctaatttttc atagtagtta
     6181 ttaaacggat gtctaatgac taataaatca cagccaatac tttctaaagt tttacatgtg
     6241 tcatataaag attcaccttt tgaaacagat gatgttgatg tttcaaagct aatcgttttt
     6301 aaccctagct taagttctgc catttcaaaa ctacattttg ttcgagtaga attttcaaag
     6361 aataaatttg cgacatattt cccttcaaag tttggtagtt gacgttcacc agatttaaat
     6421 tgacttgcct tttggataag tttgtatatt tgatctgtag ataaatgttc cattgataat
     6481 aaatgattca taaaagcgcc ctccttaatt tgtaaatctt aattgttttg ttttttctct
     6541 ttaggtaaga ttaagttcaa aataattcct gaaagtgcag ctaatgccat cccctcaatt
     6601 tgaaggttga taccaatttc ttttaagtta aatactaaat taccgatacc tacaactaaa
     6661 attacagaag ctataactaa atttcgattg ttcgcaaaat ctactttgct ttcaactaac
     6721 attcttaagc cacttgctgc aataataccg aaaagtaata tagatactcc tcccataact
     6781 ggtgtaggta tagaagaaat taatgcagtg aacttaccaa tgaatgctaa aacaattgct
     6841 ataactgctg caccaccaat gacgtaaata ctgtatattc tggtaatcgc taatacaccg
     6901 atattttcac cgtatgttgt acttggtggt ccaccaataa tactggcaaa cattgtagaa
     6961 acaccatcac caatgattga tttatcaagt cctggctttt caaagaagtt tctacctacg
     7021 attttattca ataccatttg gtgcccaata tgttcactta ctgttacgaa cacaatcgga
     7081 atcattacaa gtacaagtcc taagtgaaat gaaggtacat aatctttaaa tggcagataa
     7141 atatgaggga aatctatcca ttttgctgac ataattgcat caaatttcac aatccccata
     7201 aaaatcgcta ccacataacc tacgataata cctacaagaa ctggaattaa agataggaat
     7261 cctttaaaaa atccttgaac gactattgtt acaagcaagg ttatcattgc aacaattaag
     7321 aaactgatat tgtaaccttt catatctcca ggattttcat acattgccat attgactgca
     7381 gtaggcgcta agcttaaacc aattaccatg atgactggtc caacaacaac tggtggtaat
     7441 aatttcatta accatgctgt cccacttaat ttgattagaa tcccgatgat gacgtacata
     7501 acaccactca tgaataatgc tacaagcatg tctcctaagc tatgcgtact taatcccgtg
     7561 ataattggcg tgataaatgc aaagctagat cccaagtatg ctggtatttg cgccttcgtt
     7621 attaagatat aaagtaatgt accgattccc gaagctagta acgctgctga tattggtagt
     7681 cctgttaaga atggtactag tactgttgcg ccaaacatcg caaataaatg ttgtaagctt
     7741 aaaaatgccc attgcgctgg ttgtggtttt tcatttacat ctagtacggg ttttactgtt
     7801 cgttcaaaca tttcatcatt ttgcataata ttcatttcct ccgataataa aaaaatctct
     7861 ttacatcagt atatgtaaag agacaaaaag tgtgacaagt tgctacaagt cattttcgtc
     7921 catagaaatt gacttatagt tgtcgaacat gagggtatta ttagataaac aagcatatga
     7981 aaacttattt atcattcaac tcccccacct ttttcagtct ctcgtactga attaaaaggg
     8041 gtattattta attataactg catttctttg atccatttct tctaaataga cacttaccgt
     8101 ttcctcttta gaagtaggta tatttttacc aacaaaatct gctcgaattg gtaactcacg
     8161 atgtcctcga tcaaccaaag cagctaaacc aattttaata ggtctagcat ttagcaaaat
     8221 agcatcaagt gaagcacgaa ccgttcgacc agtatacagc acatcgtcaa taatgatgac
     8281 tactttatct gtaatatctg tgtcgatgtc tattgcgtct tttgtcgtaa gtgatgacat
     8341 gtgctctata tcatctctaa agtatgtaat atcaattgtt ccagtaggta tacgttgttg
     8401 ctcaatttga tgaattttat cttgtatacg attcgctaaa tattcacctc ttgttttgat
     8461 acctaaaaga attaaattat cagtaccttt gttatactcc aaaatttcgt gagcgattct
     8521 cgtcactgta cgttgtattg cggcatcatc cattatgata cgttcagaca tattctcacc
     8581 ctttcaaaat ttagaaacaa aaaacctcat gtctttaata tcgacatgag gtttagtata
     8641 tacaatcagc ctatttacat agaagaatcg tagtattact acttattctt gcatatgtga
     8701 aagtgttatg atcattttag tatatatcta tattttcatg tctttgacgt ctctctggac
     8761 gctcaattaa agactcttta tgttgtactt aaactgttat aaggataata ctaatcaggc
     8821 gctttgtcaa tctatattga aaaattaacc atactcagta aaaatgttaa ccttaatcta
     8881 ttttataatg tgttttcatt taaaaaaacg aaaacgataa acacttatag tttacataat
     8941 aatgttatcg taattatagc acgacacaat tatgcatctc tttttcgaat tgtatctaat
     9001 aaatcttcaa agtcttgtgg taattcagca tgtctttcaa tatattcact tgttactgga
     9061 tgttcgaatc caataagtcc agcatgtaga gcttgaccac caatatccaa tgtctttttc
     9121 ggtccatact ttggatcacc aactaatggg aagccaatat atttcatgtg aacacggatt
     9181 tgatgcgtac gtcctgtttc aagttgacat tcaacaagcg tataatcttt aaaatgttct
     9241 agtacgttaa aatgtgtcac tgcttcctta ccatcatcaa caacagccat agattgacga
     9301 tcatttttgt ttctaccaat tggcgcatcg attgtaccgt aatcatgagg aatattcccg
     9361 tgaactaaag cgatatattt tcttttaaca gatttatcca ttaattgttc tacaagccca
     9421 cgatgagcaa tatcattttt agcaaccatt aataaaccag aagtatccat atctatacgg
     9481 tgaacaatac ctggacgaat ttctccatta atacctgaca aatttttaat ttgatacatt
     9541 aaaccattaa ctaatgtatt ggtataatgc cctggtgatg gatgaactac catgcctttc
     9601 ggtttatata caactgcaac atcgtcatct tcataataaa tatctaaatt taaattttca
     9661 ggtagaatat cagcttcaac cacttctttt tcagtgacaa ctatatgatc attaagtttc
     9721 actttataat tagatttaac aactttatcg tttgcaacga ctaaacctgc tttaatccaa
     9781 tcttgtatct ggttacgaga ccaatcatta tttaattcag gcagcaactt atctacacgc
     9841 atacctgttt gttctttatc tgtaatgtta aattcataag tctccattac ttaacctcct
     9901 tctccttttt attggaagta tcctttaata aggcaataat aattaatatt acaccaattg
     9961 ttaaacttga atctgcgata ttaaatattg gaaaatcata accaaaaata tttgtatcaa
    10021 taaagtcaac aacttctcct gttaaaattc tatcaataaa gtttccaagt gcacctgcaa
    10081 aaagtaaact aatagcaact tgc
//
LOCUS       DAMQYT010000085        10042 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMQYT010000085 DAMQYT010000000
VERSION     DAMQYT010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10042
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.085"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..625
                     /locus_tag="PGA33_001573"
     CDS             <1..625
                     /locus_tag="PGA33_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDG6473381.1"
                     /translation="KKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIKQLIRM
                     NNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFENQVRII
                     EGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYHGFVSD
                     IGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            628..1551
                     /locus_tag="PGA33_001574"
     CDS             628..1551
                     /locus_tag="PGA33_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDG6473382.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            1563..2831
                     /locus_tag="PGA33_001575"
     CDS             1563..2831
                     /locus_tag="PGA33_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDG6473383.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            2831..3454
                     /gene="udk"
                     /locus_tag="PGA33_001576"
     CDS             2831..3454
                     /gene="udk"
                     /locus_tag="PGA33_001576"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDG6473384.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            3482..3958
                     /gene="greA"
                     /locus_tag="PGA33_001577"
     CDS             3482..3958
                     /gene="greA"
                     /locus_tag="PGA33_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDG6473385.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            4284..5018
                     /gene="pxpB"
                     /locus_tag="PGA33_001578"
     CDS             4284..5018
                     /gene="pxpB"
                     /locus_tag="PGA33_001578"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDG6473386.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     KFIQIERDISDGNFNVDDWVVIENVN"
     gene            5008..6018
                     /locus_tag="PGA33_001579"
     CDS             5008..6018
                     /locus_tag="PGA33_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDG6473387.1"
                     /translation="MSIKILQPGLFSTVQDLGRIGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSINASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPSIAESYATHTRSSIGGFKGRKLLADDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            6020..6469
                     /locus_tag="PGA33_001580"
     CDS             6020..6469
                     /locus_tag="PGA33_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HDG6473388.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMIGTFFLQDSKELTEPIVNVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            6483..7844
                     /locus_tag="PGA33_001581"
     CDS             6483..7844
                     /locus_tag="PGA33_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDG6473389.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIENLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKVTLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            7844..8596
                     /gene="pxpA"
                     /locus_tag="PGA33_001582"
     CDS             7844..8596
                     /gene="pxpA"
                     /locus_tag="PGA33_001582"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDG6473390.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIANVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            8609..9838
                     /locus_tag="PGA33_001583"
     CDS             8609..9838
                     /locus_tag="PGA33_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDG6473391.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
BASE COUNT         3590 a         1384 c         1986 g         3082 t
ORIGIN      
        1 taaaaaatat ttaatagatt tacatcaaca tcaaaatagt tcaatcgaag ttttgcgtga
       61 atttgccgag gtaaatgaag tgccaattgt agatcgttta acattagatt taattaagca
      121 attaattcgt atgaataatg ttaaaaatat tttagaaatt ggtacagcaa tcggctatag
      181 ttctatgcaa ttcgcttcta tatctgatga cattcatgtc acaacgatag agcgtaatga
      241 aacgatgatt caatatgcta aacaaaattt agctacttat cattttgaaa accaggttcg
      301 aattattgaa ggtaatgctt tagagcaatt tgaaaatgta aatgacaaag tttatgatat
      361 gatatttatt gatgcagcaa aagcgcaatc aaagaaattt tttgaaatat atacaccact
      421 tttaaagcac caaggtctcg taattacaga taatgtttta tatcacggtt ttgtatcgga
      481 tattgggatt gttagatcga gaaatgtaag acaaatggtt aaaaaggttc aagattataa
      541 tgagtggtta ataaagcaac caggatatac aacgaatttt ttaaatatag acgatggatt
      601 agcgatttca attaaaggag aatgactatg acagaattac ttgtcacacc caagtcatta
      661 agccatatgg aaacacttat tgatttaggt gcagatgctt ttgtaattgg cgagcaaaag
      721 tttggtttaa gacttccagg ggaattcaat agacaacaaa tgacagaggc tgtcgcatta
      781 gctcacaaaa acgataaaaa agtatatgcg gcagtgaatg gattatttca taattatcat
      841 ttagatgctg tagaagatta tattaatttt ttacatgaaa tacgagttga cagaattatt
      901 ttcggggatc cagcagtagt aatgtatgtg aaggcgcaag aaaatccaat accacttcat
      961 tggaatgcag agacgttagt aactaatcat tttcaatgta attactgggg taaaagaggc
     1021 gcatcaagag ctgtcttagc acgtgagctg aatttggatg aaattattaa tataaaagaa
     1081 aattcaaatg tggaaattga ggtgcaagtt caaggaatga cttgtatgtt tcaatcaaag
     1141 cgtatgctac taggcaatta ttatacgttc caagatcgcc aaatgaaaat tgaacgtcgc
     1201 aatgatgaac aatctttatt actttatgat gaagaaagac aaaataatta cccagtttac
     1261 gaagattaca atggtacgca cattatgtca ccaaacgaca tttgtttaat tgaagaatta
     1321 gcaccatttt ttgaggcggg tatagattca tttaaaatcg atggtattct acaaacggaa
     1381 gaatatatta atgtggtaac ggaacagtat cgtcaagcga tagatttgta caatgaagat
     1441 cctgaaatct atgaagatga gaaatttatg ttgatggatc caattgaaga aattcaacct
     1501 gatcatcgac catttgacga aggtttctta tataaacaaa cagtatatta aggaggttaa
     1561 tcatgaagac aatagaagag attaaatcaa ctcctaaaac agttatgaag aaaccagaat
     1621 tattagcacc tgctggaaac ttagaaaagt taaaaatagc agtacattat ggcgctgatg
     1681 ccgtattttt aggtggtcaa gaatatggat tacgttcaaa tgctgataat ttcacgatgg
     1741 aagaaatagc tgaaggtgtt gaatttgcga accgttacgg tgccaaaatt tatgttacga
     1801 caaatattat tgcacatgat gagaatattg aaggtctaga atcatatttg cgtaatttgg
     1861 aaaagactgg tgcgacaggt atcattgttg cagatccttt aattatcgaa acatgtaaag
     1921 aagttgcgcc aaaacttgaa attcatttat ctactcaaca atcactttct aattacaaag
     1981 ctgtagaata ttggaaagaa gaaggattgg atcgtgttgt attagcacgt gagaccggcg
     2041 cgatggaaat gcgtgaaatg aaggaaaaag tagatattga aatcgaagca tttattcatg
     2101 gtgctatgtg tatcgcctat tcaggtagat gtacattaag taatcatatg actgcaaggg
     2161 attccaacag aggcggttgc tgtcaaagtt gccgttggga ttatgaatta ttagaagttg
     2221 atgataatgg tgaacttgat gttttttata atcaaggtga agttacaccg tttgcgatga
     2281 gtcctaaaga tttaaaatta atcgaatcaa ttcctcaaat gatggatatt ggtgtggact
     2341 cattaaaaat tgaaggacgt atgaagtcaa ttcattatat tgcaacagtt gtctcagtat
     2401 atcgtaaagt cattgatgcg tatgcggcag atcctgacaa ctttaagatt aatccggaat
     2461 ggttaataga gttagataaa tgtgcaaata gagacactgc accagcattc tttgagggaa
     2521 cacctggtta tgaagaacag atgtttggtc aacaacaatc taaaaaatca ccatttgatt
     2581 tttgtggttt ggtattagac tataatgaag atacaaaaat tgcgactatt caacaacgaa
     2641 ataactttaa accaggtcaa gaaatagaat tctttggtcc agaaattgaa acatttacac
     2701 aagtagtaga agcaatttat gatgaagaag gtaacagctt agacgcggcg cgccatccat
     2761 tacaaatcgt tcaaattaaa gtggatcgcc cgatatatcc aaacaacatg atgagaaagg
     2821 aaattggcta atgaaagcta ctacaatcat tggcatagct ggtggatctg gctcaggaaa
     2881 aacaactgta actaacgaaa ttatgaaaaa cttagaaggt catagtgtcg ctttacttgc
     2941 tcaagattac tattataaag atcaaaagca cttgactttc gacgagcgcc tagaaaccaa
     3001 ttatgaccat ccatttgcat tcgataatga tttattaatt gaaaatctta aagacttgaa
     3061 aaatggtaaa gcagtagaag taccgacata tgattatgct agtcatacaa gaagtgacat
     3121 taccattgat tttaaaccta aagatgttat tatcgtagaa ggcattttcg ctttagaaaa
     3181 taaggtatta cgtgatatga tggatgttaa aatatatgtt gatacagatg cagacttgag
     3241 aatattacgc cgtttaacac gagatactaa agagcgtggg cgttcaatgg actctgttat
     3301 caatcaatat ttaagtgttg ttagacctat gcatgaccaa tttattgaac cgactaagaa
     3361 atatgctgat ataattattc ctgaaggtgg gagcaataaa gttgcaatag atattatgac
     3421 aacaaaaatt cagtctttag ttagcaagca atagtataat tcagtaaagg aagatgacaa
     3481 tatggaaaat caaaagcaat atccaatgac tcaagaaggt tttgaaaaat tagagcgtga
     3541 acttgaagaa ttaaaaacag ttaagcgtcc tgaagttgta gagaaaatta aagttgcacg
     3601 ttcatttggt gacttatcag agaactctga gtatgatgca gcaaaagatg aacaaggatt
     3661 catcgaacaa gatattcaaa gaattgagca tatgttaaga aatgcattaa tcattgaaga
     3721 tactggagat aacaacgttg ttaaaattgg taaaacagta acgtttgtag aattaccagg
     3781 tgatgaagag gaaagttatc aaatcgttgg ttcagctgaa tcagatgcat ttaatggtaa
     3841 gatttcaaat gaatcaccaa tggctaaagc gttaattggt aaaggtttag atgatgaagt
     3901 tcgtgttcca ctacctaatg gtggcgaaat gaacgtaaaa attgttaata tccaataaat
     3961 gatcaaatag attatttaat tgcgcgattg aagtgtgtta atgatacttc ggtcgtgctt
     4021 ttttatattc aaaatcaaaa aatcggacag atgaaaagta aaaattttcc atcagaccga
     4081 atgatagcag aacctaaatt agtgtaagag ttacaatatt aactactcca ttttaaaaaa
     4141 gcaaaatatt tccgagaaaa caaattgatt atcatttgtt attgtatgcg atttgaatta
     4201 cttattaaac aacttgtgct tctcaatgat acaatgaaaa ggtattcaat actggtttat
     4261 tgcaatacaa agggggattt aaagtggatg taagatttat taacgagcag acaattatga
     4321 tttattttga aaataaaata tccgaagaga catatcgtaa tgtcacagca atggtacggt
     4381 ggatacgaga aaaagaaata ttagaaattc aagatattgt cccttcgtat cgagcagttc
     4441 taatatattt tgatgaacaa gctataacat cttctaaatt aattgaaaat ttagaactta
     4501 acaaatttaa tgaaaaaaat gttcatgctg tgaatcaaac caatcgtatt attaaaatac
     4561 ctgttcaata tggtggtaca tatggaccag atattgaaga agtagcaaag cataatcgaa
     4621 taactgttga gcaagttatt gaaaaacata caagtaaacc ttatttaata tatatgctag
     4681 gatttatgcc aggatttcca tacttaggcg gacttgatga acaactacat acacctagac
     4741 gtaatcagcc aaggttaaaa attcatgcag gttctgtagg tattgcgaat aatcaaacag
     4801 gactatatcc atcagattct cctggcggat ggcaaattat tggacgcaca ccgttaaaag
     4861 tatttagcag cgagcgtgaa ccaatgtcaa tgtacgaagc aggagaatgg atacaatttt
     4921 atgcaataga tgaacagaaa tttatacaaa tcgaaagaga tatatcagat ggcaacttta
     4981 atgttgatga ctgggtggtg attgaaaatg tcaattaaga ttttacaacc aggtctcttt
     5041 tcaacggtac aagatctagg aagaataggt tatgaacata ttggattttc aggcgcaggt
     5101 gccatggatc aatttagttt taaggttgcg cagtcattaa ttaacaatga tggtccagcg
     5161 attgaatata ctttgattgg tcctaccatt caatttaata cgcaaaatac atttgttata
     5221 accggtggta gtattaatgc ttcgctaaat aataaaacta tatcaatgaa ttctgtcata
     5281 ttagctgaga aaggtgacat tttaaaaata ggtgctataa ccaaaggtgc acgcggttat
     5341 cttacttttg gtcattctat caacgtacct tctattgctg aaagttatgc aacacatacc
     5401 cgaagcagta taggaggatt caaaggtaga aagttattag ctgatgatgt aataacagtg
     5461 aaaataaata atgactttaa agaaaacatt ggaaagacga ttcatttaca ggacgattta
     5521 ttgccagaaa ataatattat acacattctt caaggacctc aattcgaggc attttctgaa
     5581 gaggctagag cgaaaattgt aaatcatcca tatttaatta ctgaacaatc agaccgtatg
     5641 ggctatcgtt tggaaggtga cagcgttgca ccaattaatc aagcagatat catttctgaa
     5701 ccggttgctc ttggtagtgt tcaggtacca aatgatggtc aacctattat tctgcttaat
     5761 gataaacaaa cgataggtgg ttataccaaa attgcaactg tatgcaaatt tgatctgcca
     5821 aagttagcac agatgaaacc acaagataca attcaattta aatggatatc attccaagaa
     5881 gctgtagata agaatcggga acaaatgtct ctgtttaatg aaattcttaa gagtcatcaa
     5941 aagactccaa tttttgatac atctagcttg cgacatactt cgaaaaaatt agcaacaatt
     6001 ttaaaagggg atttgtaaaa tgaatattga aaaaatcgaa caaataatca aattagtgaa
     6061 ggaaaatgat gttaagaaat ttaaatataa aaattttgaa gatgaaatag aaattgactt
     6121 cactgactcg aatcatttgg ctgcacacag taatcaatca aatcaaagta tgaacaataa
     6181 tgatttgaca gcttcaaaag cgaatgataa ctccgatgtt tcgacaaatg attatcatga
     6241 cattaaatca ccaatgatag gtacattctt tttacaagat agtaaagaat taactgaacc
     6301 aattgtgaat gtcggtgaca aagttaacaa gggagatatt ataggatatg ttgaagcgat
     6361 gaaagtatta aacgaggtaa caacagatgt tgctggagaa attactgaaa tagtagctga
     6421 tcatggcaca aatgttgaat acgaccaagt tttggtacgt attaagtaaa gggggtaagt
     6481 aaatgcttcg ttgtttaatt gcgaacagag gtgaaattgc tgtaaggatt ataagagctt
     6541 gcagagaata tgggattgaa actgtagcag tttatgcaaa aggggacgag caaagcttgc
     6601 atgtacattt agcagatcaa gctatatgta ttggggaagc taatgcttta gatagttatt
     6661 taaatattga ccgcatcata tctgctgcac aaatcactgg tgctaatgca attcacccag
     6721 gatatggctt tttatcagaa tcaacaaaat ttgctcaaac cgttgaggaa caaggcatag
     6781 cgtttatagg gcctactaaa aaaacgatgg aaatgatggg ggataaaata acagcaagac
     6841 aaacagttca tcatgcaggt gttcctgtga ttccaggatc aaatggtgct gtgaatcatg
     6901 tgtctgaaat tgagaatctt gccaaagaca tcggttatcc agttgttatc aaagctgcca
     6961 gtggcggtgg tggtaaaggt atacgtattg taaagaaagc tgaagattta gaaaaggcat
     7021 ttaaagaagc taaaagtgaa ggaaaaaaat actttgatga tgatcgtgtt tatgttgagg
     7081 cttttatacc tgttgcaaaa catgttgaag ttcaagttat gggagatggg caggataatt
     7141 atgtacattt aggggaacgt gattgttctg tacaacgtaa aaatcaaaaa ttaatcgaag
     7201 aatctccttg tgcagcatta actgaagaaa gacgacaaca aatatgtaac gatgctgtta
     7261 aagttgcacg tgcagcaaat tatagaagtg caggtacgat tgaattttta gtaactgata
     7321 ccgcacatta ctttattgaa atgaatgctc gaatacaagt tgaacatact gttacagaga
     7381 tgcgagcaga acgtgatttg gttgcagcac aactgtattt attggaacat aatcatttac
     7441 ctttttcaca aagcgatatt caatttaatg gtcatgtgat agaagcgaga ataaatgccg
     7501 aaaatcctga aaagaaattt caaccaacac caggtaaagt gacagcgtta catttacctc
     7561 aaggctttaa tgtgcgagtc gattctttac tatatcacgg ttatcaagtt tcaccatatt
     7621 atgattcttt agtagctaaa gtaattgtaa aatctcatga tagagcttca gcaattgata
     7681 agttgaaagt caccttagat gaaatggtga tagatggatt ttctacaact gccgattttt
     7741 tatatgcagt tttaaattat ccactatatc gtgatggaga tgcaaaagat gttgatatta
     7801 aatttttaga aaaacatcaa attgttaaag gggtggaatt ataatgcgag ttgatttgaa
     7861 ttgtgattta ggcgaagcat ttggaaatta ttcctttggt ggtgatcatc aaattattcc
     7921 gttaattaca agtgcgaatg ttgcttgtgg ttttcacgct ggtgatgaaa atgtaatgaa
     7981 tgaaacggta aaacttgcca aagcacataa tgttgcagta ggtgcacatc ctggtttacc
     8041 tgatttgaaa ggctttggca gacgaaatat agatatctct aacgacgaga tttataattt
     8101 gatgatttat caattaggtg cattacaagg gttttgtcgc attcatcaag ttaaaattaa
     8161 tcatgttaaa ccgcatggtg cattgtatca gatgggtgca aaagacagag aaatagcaaa
     8221 cgttatagca caagctgttt atgactttga tccatcacta gtgttagtag gattagcaaa
     8281 ttcatatcta atttcagaag caaagaatgt cggattaatt acagcttctg aagtgtttgc
     8341 tgatagacga tacgaagatg atgggcagct cgttagtaga aaagaaagtg atgctgtgat
     8401 tactgatact gacgaagcac ttaagcaggt tttaaagatg gtgaaggaaa ataaagttat
     8461 ttcaaaaaac aataaggaag taacgttaca agcagataca atttgtgtgc atggtgatgg
     8521 agaacatgca ttattatttg tttcgaaaat tagagaaatt ttaatgaaag aaggcattga
     8581 tattcaatcc ttataggggg ctaaaaaaat ggggagaaat ttaaagctta aaaaagaaag
     8641 tgactttgaa ttcacaaaaa atcataaaag gttattatta ggttctgtat ttttgatggc
     8701 aacttctgca attgggccag catttttaac gcaaacagca gtatttacat cacaattttt
     8761 tgcaagtttc gcatttgcca tattactgtc tatcatcatt gacattggtg cacaaattaa
     8821 tatatggcgc atattagttg taactggttt aagaggtcaa gaaatatcaa ataaagttgt
     8881 tcctgggctt ggtactgtta tctcaatatt gattgcattt ggtggtctag cttttaatat
     8941 tggtaatatt gctggtgccg gtttaggttt aaatgcaatt tttggattag atgtaaaatg
     9001 gggcgcagct attactgcaa tctttgcaat attaatcttt gtaagtaaaa gtggccaaaa
     9061 aattatggac gttgtttcaa tgattcttgg tattgtgatg attttagttg tggcatatgt
     9121 gatgtttgtt tctaatccac cttatggtga tgcttttgtg catacatttg cgccagaaca
     9181 tccaatgaaa ttagtcttgc ccatcattac gttagttggt ggaactgtag gtggttatat
     9241 tacctttgca ggtgcacatc gtatattaga ctctggcatt aaaggtaagc aatatttacc
     9301 atttgtaaat caatcagcaa ttgctggtat tttaactaca ggtattatga gaacgttact
     9361 attcctagcg gtattaggag ttgttgtaac aggtgtgaca ctaagttctg aaaatccacc
     9421 agcgtcagtt tttgaacacg caattggacc aattggaaag aatatttttg gtattgtgtt
     9481 atttgctgca gctatgtcat cagtaattgg ctcagcatac acaagcgcaa catttttaaa
     9541 aacacttcat aaatcactta acgaaagaag taatttaatt gtgattgtgt ttatcgttat
     9601 ttcaacaatg attttcttat ttattggaaa accaatcagc cttttaatta tagcaggcgc
     9661 gataaatgga tggattttac ctattacgtt aggtgcaatt ttgattgcaa gtaaaaagaa
     9721 atcaattgtt ggtgattata aacacccaaa ttggatgttc atctttggta tcgttgcagt
     9781 acttgttaca atattgacag gtatattctc attcaaagaa gtacttcagt tattttaaaa
     9841 gacatttcga ctaatagcat cactgtaaat gttgttagta ttaggcaaga tatattttga
     9901 tttccggtga ttcatttata aagcataatt agatatattt taagggagag tggtgcatga
     9961 caaatgtact gctctttttg ttgtgatgaa aaatcttttt ctattgattg agaaaatagt
    10021 tatttatata gtttttaaag aa
//
LOCUS       DAMQYT010000086        10027 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMQYT010000086 DAMQYT010000000
VERSION     DAMQYT010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10027
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.086"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            354..1313
                     /locus_tag="PGA33_001584"
     CDS             354..1313
                     /locus_tag="PGA33_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDG6473392.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(1359..1490)
                     /locus_tag="PGA33_001585"
     CDS             complement(1359..1490)
                     /locus_tag="PGA33_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473393.1"
                     /translation="MRYVTMRQFIKRTVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(1554..1769)
                     /locus_tag="PGA33_001586"
     CDS             complement(1554..1769)
                     /locus_tag="PGA33_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HDG6473394.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            1934..2485
                     /locus_tag="PGA33_001587"
     CDS             1934..2485
                     /locus_tag="PGA33_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6473395.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVENKVAGCIISYSGENELKYEKAWELLDLPEKIKQYGTPLP
                     VKEAKDDEYYIETIATFAAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(2537..3475)
                     /locus_tag="PGA33_001588"
     CDS             complement(2537..3475)
                     /locus_tag="PGA33_001588"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDG6473396.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            3657..5018
                     /locus_tag="PGA33_001589"
     CDS             3657..5018
                     /locus_tag="PGA33_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDG6473397.1"
                     /translation="MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
                     MDLFNNISDIDVDEQIGGITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            5005..6678
                     /gene="menD"
                     /locus_tag="PGA33_001590"
     CDS             5005..6678
                     /gene="menD"
                     /locus_tag="PGA33_001590"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDG6473398.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTVNRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            6665..7468
                     /gene="menH"
                     /locus_tag="PGA33_001591"
     CDS             6665..7468
                     /gene="menH"
                     /locus_tag="PGA33_001591"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDG6473399.1"
                     /translation="MTHYKFYEANVETNQVLVFLHGFLSDSRTYHNHIEKFTDNYHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            7461..8282
                     /gene="menB"
                     /locus_tag="PGA33_001592"
     CDS             7461..8282
                     /gene="menB"
                     /locus_tag="PGA33_001592"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDG6473400.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(8521..8850)
                     /gene="sspC"
                     /locus_tag="PGA33_001593"
     CDS             complement(8521..8850)
                     /gene="sspC"
                     /locus_tag="PGA33_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HDG6473401.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(8888..10027)
                     /gene="sspB"
                     /locus_tag="PGA33_001594"
     CDS             complement(8888..10027)
                     /gene="sspB"
                     /locus_tag="PGA33_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HDG6473402.1"
                     /translation="MIMVSMLILSLGAFANNNKAKADSHSKQLEINVKSDKVPQKVKD
                     LAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYYYPVIKDGKIVYTLT
                     LSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLTDEKGFYFEEDGKVR
                     LVKATPLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQEDQVQYENTLKNFK
                     IREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSEQDLPNCATFPNQMI
                     EYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPNDPHLGHALAVVGNA
                     KINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIGY"
BASE COUNT         3566 a         1413 c         1842 g         3206 t
ORIGIN      
        1 agaaagatgt ttaagtcatc agttactttt tgtatttata aataaagatt agtacagcat
       61 agtgcaaaga ttatagggaa atgaattaac tattgcatcg ctatttatga atcgtaaaca
      121 ctattatgaa attgcgagag tagtatggac aattagaatg atggtttgag ataaatgagt
      181 gttattaacc agtgcgtcat tttcaaaatt agatgccatt tgctgtttat aataagaaga
      241 tttgataatt aactatgata agaaaaaata ataatcccct aaatacatta taaacaaatt
      301 aaatgataaa ataataatat atatttctac taaagttgtt tggaggtaaa tgtgtgaata
      361 ggaatatcgt taaactagtt gtgttcatgc taattttagt tgtagcagta gcgggttgtg
      421 gtcaaaaaga tactgaagag aaaactgaaa tgacgacaat aaaagatgaa ttaggaactg
      481 aaaaaattaa gaaaaatcct aaacgtgttg ttgtattaga atatagtttt gctgattatt
      541 tagcagcatt agatatgaaa cctgttggta ttgcagatga tggcagcact aaaaatataa
      601 caaagtcagt aagagataag attggggcat atgaatcggt tggatctaga ccgcaaccga
      661 atatggaagt gataagtaaa ttaaaaccgg atttgatcat tgcagatgtt agcagacata
      721 agaaaatcaa atcagaattg agcaaaattg ctccgacaat catgttagtt agcggtacgg
      781 gagattataa tgcaaatatt gaagcattta aaacagtcgc taaagcagta ggcaaagaga
      841 aagaaggcga gaagcgtctg gaaaagcatg ataaaatatt agcggagatt agaaagaaaa
      901 ttgaacagag tacgttaaaa tctgcatttg cattcggtat ctcaagagca ggtatgttta
      961 ttaataatga agatacattt atgggacaat tcttaattaa aatgggtatt caacctgaag
     1021 tcacaaaaga caaaactacg catgttggtg aacgcaaggg tggtccttat atatatttaa
     1081 ataatgaaga acttgccaat atcaatccaa aagttatgat tttagccact gacggaaaaa
     1141 cggacaaaaa tagaacgaaa ttcattgatc ctgcagtttg gaaatcatta aaagctgtga
     1201 aagataacaa agtttatgac gttgaccgaa ataagtggtt gaaatcaagg gggattatcg
     1261 caagtgaaag tatggcagaa gatttagaaa aaattgcaga aaaagcaaaa taaaaataca
     1321 gcgctactcg taaatcatat aagagtggcg ctgtattttt aattatgttt attatctgtc
     1381 ggatgtgatg atttacctga aagtttattt cgaataaatt taattacata accgacaagg
     1441 attgttttaa cagttctttt aatgaattgg cgcatcgtta catacctcat ttctctatat
     1501 cttacgaact atatacccat tcatatatgc tttttaaacg tcattgtcac aatttaattt
     1561 ttagggaata taatataacc atctttatct gcttttttag taaaaatgac aaaaattgca
     1621 tgtattattg agatgatggt agggatacct gtccagaaaa ataataagtg aaaaagacct
     1681 tgtccaaatt tatcagcata aaatttatga atacctaaac ctccaagaaa taatgcaaca
     1741 ataacataaa tggctttatt gactttcatt tgtaaatcct ccttaactat aattctactt
     1801 aaattcgttg tgaaaaccaa tatttctaac tttagaattt tcaaactttc taaaattata
     1861 agtatatctt tttaaaataa gctagaattt ctatataata aatgttaata acgtaaaagg
     1921 gaatgatgac atagtgatac gtcaagcacg tccagaggac cgatttgata ttgcgaagtt
     1981 agtttatatg gtttgggatg atatggaatt agaattggta aagcatctac ctaaagacat
     2041 ggtattagat gcaattgaaa aaagctgtgt tgatgcaaca tatcgaactt tttatcagca
     2101 tattttagtt tatgaagtag aaaataaagt agcaggttgt attattagct atagtggtga
     2161 aaatgaattg aaatacgaaa aagcatggga actacttgac ttgccagaaa aaataaaaca
     2221 atatggcacg ccattacctg taaaagaagc taaagacgat gagtattata tagaaacaat
     2281 tgcgacattt gcagcatata gaggtagagg catcgcgaca aagttattaa cgtcattact
     2341 tgaatcaaat acacatgtta aatggagttt gaattgcgat attaataatg aagcagcatt
     2401 aaagttatat aaaaaagtag gctttatatc tgatggacag attgaattat acaagcacat
     2461 gtatcatcat ttaattgtta aataaaaaaa ctcgacagtt cgatgtaagt cgattgccga
     2521 gtagtatcat tttctattaa atgcctgcaa ataatgcact aatataaata cctaatgcat
     2581 ataataaacc gaaaaatgta tttgttttac cagcagcagc cattgctggc atcattgtag
     2641 gcggtgtatc attcttcttg aaacgtctga taactttaac aggcattggg aatgataaca
     2701 acgcaagtaa gtaaaataat gagccaccag gtttaataat gatcgtaagt acaataaagg
     2761 cataagcgat aaagtacatg attgccataa atgttaaaga agcattttta cctaatagaa
     2821 tgggtaaagt tttgcgacca cttgctttat ctttgacacg gtcgcgaata ttgttagcca
     2881 tattaattaa accgatagtg attactatag gtacacttaa ccaaattaca taactttgaa
     2941 tattgccagt ttgaataaag aatgcaataa cgataataaa catacccata aatacgcctg
     3001 agaataattc accgaaaggc gtccatgaaa tagggaaagg gccacctgta tataggtaac
     3061 caacagccat acatactaat ccaactggta ataaccaaaa tgaagagtta gcagctaaaa
     3121 acaaacctaa tattgctgct aagatgtaaa atgcaatggc taatcgtagc acaagctctg
     3181 ggctcatacc gttgcgaaca atggcaccac caatgcctac agattcatga tcatcaaggc
     3241 cttttttata atcatagtat tcattaaaca tattagttgc tgcttgaata agtaagcatg
     3301 ctagtaacat ggcaatgaat aggctgattt taatatgatc ttcgctacca agaaaatata
     3361 ttttagatgc tgctgtacca actaaaacgg gtactacgga agcagttaat gtatgaggac
     3421 gcattaaatg ccaatatttc ttaactgtag aatattgctg atattgatta ctcattgaat
     3481 acaaaacctc tttaaatcat attttttata acaaagatat tttaatagaa agtagcataa
     3541 aggtcaattg acgtgaaaaa ttgcaagata agtttaagca attcttgtaa cacaattatc
     3601 caaaaatgat acaatataat aatgagtgaa aaaagacgaa gaaagaagtg aaatggatgg
     3661 ctacgggcgt attagaggac gatattgtca aagagatata tggcagctca aaggaatggg
     3721 tttcagttga agtgaaatta tcacagtcac tagacccgag cacattattt catctcactg
     3781 acaatgaggc aggagatcgc ttttatatgc gtttgaatga taatcgaacg tcatattttg
     3841 gctacaaagc aattcaatta ttcaaaaata attctaaaaa taaacaatct atttttaaag
     3901 actgggaaaa attaaaacat aacatcacat ttatacatcc gcaatctgag aaacatcatc
     3961 ttcgagttgt tggagggttc caattttcaa gtcataaatc agatgatgaa tggcgagagt
     4021 ttggactaaa tcattttgta ttacctgaag ttttaatttc aactgataat aatgggacat
     4081 ttttaactta tacagttaaa agggaaagtt ttactgttga ggcattgaac gatttaatgg
     4141 atttgttcaa caatatatcg gacatagatg tggacgagca aattggggga attactagaa
     4201 atgaagatat ttataaagat gactggcgtc aacttgtagt agaagctata gaatctatta
     4261 ataatgaaga aaaaattgta ctagcacgta gacggttaat aaagttcgat aaagatatca
     4321 gtattccata tattctaaag caagcatatt ctaaagaaaa aaacagttat atattcttgt
     4381 tagaatcaca agattctata ttcttttcac aaacacctga acaattaata aaggtcaata
     4441 ataaaatact atcgactaaa gctgtagcag gtacaattaa acgttcacaa gatgaggacg
     4501 aagatacaaa aaatgttgaa gcatttttaa aagataataa aaacttaatc gaacatcgat
     4561 ttgttgttga cagtatttta catgatatta aaccttatat cactgaatta cattatgata
     4621 agacgcctaa aattctaaaa aatgatcatt tatatcactt gtacactgaa ataaaggcgc
     4681 cactgaagga tgattcgtat attagtttaa ttgataattt acatccaaca cctgctttag
     4741 gtggctatcc aaaagaattt gcaatggatt ttattgagca gaaagaattt ggtacgcgag
     4801 gattatatgg tgcaccggtt ggctatatag atatatacga tgattgtgaa tttattgttg
     4861 caattcgttc gatgcttatt aagaaagcac aagcaacttt atttgctggg tgtggcattg
     4921 ttaaagattc tgatccagat agtgaattgg cagaaacgaa ccttaagttc acacctatga
     4981 tgaatgcatt aggagtcgat atgaatggga aatcataaag cagctttaac gaagcaagtt
     5041 tttacatttg catctgagtt atatgcgtac ggcgtaaggg aagtagttat cagtccggga
     5101 tcacgctcaa cgccacttgc acttgcattt gaagcacatc caaatattaa aacatggata
     5161 caccccgatg agcgaagtgc agcgtttttt gcagttgggt taattaaagg cagtgaaaga
     5221 cctgtcgcta tattatgtac gtcaggtaca gcagcagcga attatacgcc tgcaattgct
     5281 gaaagccaaa ttagtagaat tcctttaatc gttttaacaa gtgaccgtcc gcatgaatta
     5341 agaagtgtag gcgcaccaca agcgattaat caagtaaata tgtttaataa ttatgtaagt
     5401 tatgagttcg atatgcctat tgcggatgat agtaaagaga ccattgatgc aatttactat
     5461 caaatgcaaa ttgctagtca atatttatat ggaccacata aagggccaat tcattttaac
     5521 ttgccattta gagatccgtt aacacctgat ttgaatgcaa cagaattgtt aacttctgag
     5581 atgaagattt taccgcacta tcaaaaaagt atagatgcat cggcattaag acacatttta
     5641 aataagaaaa aaggtttaat tattgtaggg gatatgcagc accaagaagt tgatcaaata
     5701 ctaacgtatt caacgatata tgatttgcct attttagctg atcctttaag tcatttaaga
     5761 aaatttgatc atccgaatgt tatctgtaca tatgatttgc tgtttagaag cggcttagac
     5821 ttaaatgtgg atttcgtaat tcgtgttggg aaaccagtga tttctaaaaa gttaaatcaa
     5881 tggttaaaga aaactgatgc atttcaaata ttagtgcaaa acaatgataa gattgatgtc
     5941 tttccgatag cgccagatat ttcatatgag atttctgcga atgatttctt taggtcatta
     6001 atggaagaca cgacagttaa tcgcgtaagt tggttagaaa aatggcaatg cttagagaaa
     6061 aaagggcgta aagaaattaa atgttatttg gaacaagcta cagatgagag tgcattcgtt
     6121 ggtgaattga ttaagaaaac atctgaaaaa gatgcattat ttattagtaa tagtatgcct
     6181 atcagagatg tagataactt gttattgaat aaaaatatag atgtctatgc gaatcgtggt
     6241 gcgaatggta ttgatggtat cgtttcaact gcactgggta tggctgtgca taaacgaata
     6301 acattattga taggtgattt atcattttat catgatatga atggactatt aatgtcaaaa
     6361 ttaaataata ttcagatgaa tattgtatta ttgaacaacg atggtggcgg tattttttca
     6421 tatttaccac aaaaagaaag tgcaactgac tattttgaac ggttgtttgg cacaccgacg
     6481 ggattggatt tcgagtatac agctaagtta tatcaattcg attttaaacg ttttaacagt
     6541 gtttcagaat ttaaaaatgc cacattgtta tctgaaactt cgacgattta tgaattgata
     6601 acgaatcgcg aagataactt taaacagcat caaattttat accagaaatt gagtgaaatg
     6661 attcatgaca cattataaat tttatgaagc aaacgttgag accaatcaag ttttagtatt
     6721 tctgcatgga tttcttagcg acagccgtac ttatcataat cacatcgaaa aatttactga
     6781 taactatcat gtcatcacta tagacttacc aggccatggc gaagatcagt cttcaatgga
     6841 tgaaacgtgg aattttgatt atattacgac gttgttagac cgaattttag ataaatataa
     6901 agataaatca ataacattgt ttggatattc aatgggtggg cgtgttgcat tatattatgc
     6961 aattaatggt cacatcccta tatctaattt gatattagaa agtacgtcac caggtattaa
     7021 agaagaagca aatcaattgg aacgccgtct tgttgatgat gcacgtgcta aagtattaga
     7081 catagcaggt attgaattat ttgttaatga ttgggaaaag ttgccattat ttcaatcgca
     7141 actagaatta ccagttgaaa tacaacatca aataagacaa caacgattgt ctcaatcgcc
     7201 acataaaatg gccaaagcat taagagatta tggtacaggt caaatgccaa acttatggcc
     7261 gcgcctgaaa gaaattaaag taccaacatt aatattagct ggagaatatg atgaaaaatt
     7321 tgtacagatt gcgaaaaaaa tggcaaattt aattcctaat agtaaatgta aattaatttc
     7381 tgctacaggt catacaattc atgtggaaga tagtgatgaa tttgatacaa tgatattagg
     7441 atttttaaag gaggagcaaa atgactaaca gacaatggga aacacttaga gaatatgatg
     7501 aaatcaaata tgaattttac gaagggattg ctaaggtaac aataaatcgc cctgaagtac
     7561 gcaatgcgtt tacacctaaa acagttgctg aaatgattga cgcattttca cgtgcacgtg
     7621 atgatcaaaa cgtttcagtt atcgtattaa ctggtgaagg tgatttagca ttctgttctg
     7681 gtggtgacca gaagaaacgt ggacatggtg gttatgtagg tgaagaccaa atccctcgct
     7741 taaatgtatt agatttacag cgtttaattc gtattattcc aaaaccggtt atcgcgatgg
     7801 taaaaggtta tgctgtaggt ggcggtaatg tactaaatgt tgtttgtgac ttaacgattg
     7861 ctgctgataa tgctattttt ggacaaactg gtcctaaagt aggttcattt gatgcgggtt
     7921 atggttcagg atatttagca cgtatcgttg gacataagaa agcacgtgaa atttggtact
     7981 tatgtcgtca atacaatgca caagaagctt tagatatggg tctagtaaat acagtggtac
     8041 ctttagagaa agttgaagat gaaactgtgc aatggtgtaa agagattatg aaacactcac
     8101 caacagcgtt acgattcctt aaagcagcta tgaatgctga cacagatggt ttagctggtt
     8161 tacaacaaat ggctggggat gcaacattgc tttattacac aactgatgaa gcgaaagaag
     8221 gccgtgatgc gtttaaagaa aaacgtgatc ctgacttcga tcaattccct aaattcccat
     8281 aagttatttt gaatatgata ttaagtcact tgcgtcgttt attagcgacg ataggtggct
     8341 tttttatttt ataagaattt aaaagggcat tttaattaat tatcataaag ataagatagt
     8401 ttgagaaaaa ttaatagaga aagaataaat gttaaagatt attattacaa tgaaaaaacg
     8461 agctggtgag aaattgaatt ctcgccaacc cgctttatga agtgaaatca tttaaattag
     8521 ttatactaag cgctcataaa cgattggtcg cgaagtgcca ataccttgtt tggatgacgt
     8581 ttgcatgagg atatgctgtg tatcatcata atcaagaata taaatgattt cttcattatc
     8641 aatagatgaa aatttaatgc gttgatgtgc cgtatctata aaaagaatat gatattgatt
     8701 gtgacttgta tcatcgccat gacgtataca aattgatttt tgtaattgat gattactcca
     8761 attaccaatg aataaattga tattggtttg ttctagatgt gtgagtttgg ttgtgtcgta
     8821 aactaaattt ataaattgta gttgatacat agttttacct gcctttaatc aatatctata
     8881 ttacttttta gtaacctatc attgaaccat accagttata atcacgattg aatgataaat
     8941 gtaataggct tgaatctgca tcttggattg ataattctgt atcccaagga ttccagtaaa
     9001 taagtttttc ttggtcatta attttagcat taccaacaac tgctagcgca tgtcctaaat
     9061 gtgggtcatt agggttttga gatacacttt gtgcaaggat cataattcct acattatctt
     9121 ttgtaagttg atcaacttgt tcatatgatg gtacgccttc ttgataatga atatctctgc
     9181 cttgtgattt accgtattca atcatttgat tagggaatgt tgcacaatta ggaaggtctt
     9241 gctcacttac ttcagggtat aatgtacgca taatatcatg tgcattataa gtgtctgtat
     9301 ttttagttgc atttaataat gctgccatac tgaatcctgc acaccatgag ttatcgaatt
     9361 gttgttctct aattttgaag ttttttaatg tattttcata ttgaacttga tcttcttgta
     9421 tcgcttcgtt ttcttcaact ttagtaggtg ttactgtatt ttttaactct tgtttcaatt
     9481 ttgctgaaac tgttttagca ctttcttttt cttttacatt accaggtaga ggcgtagctt
     9541 ttactaatct aactttgcca tcttcttcaa aataaaaccc tttttcatca gtaagaacag
     9601 tgatatttga atttttatct ttaatttggt ctaaatcttt agcgatgaag tttgaaattt
     9661 taacactgta attcatgtct tcttttgatt tgtttaaatc atctttattt ttaggactaa
     9721 gtgttaaagt ataaacaatt ttaccgtctt ttataactgg ataatagtaa ctattatctt
     9781 cttcaccatt aaatttataa attttaaaag cttcgccaag ttcatattta ccagtttttg
     9841 cattactttg tttatctaat gctttagcat aaccagcaaa ttgttgttgt gctagatctt
     9901 ttactttttg aggtactttg tcactcttaa cattaatttc tagctgttta gagtgtgaat
     9961 cggctttggc tttattgtta ttagcaaatg cgcctagtga taaaataagc attgaaacca
    10021 ttatgat
//
LOCUS       DAMQYT010000087         9906 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMQYT010000087 DAMQYT010000000
VERSION     DAMQYT010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.087"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            60..776
                     /locus_tag="PGA33_001595"
     CDS             60..776
                     /locus_tag="PGA33_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDG6473403.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            776..1249
                     /locus_tag="PGA33_001596"
     CDS             776..1249
                     /locus_tag="PGA33_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDG6473404.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEISNTFSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            1341..1457
                     /locus_tag="PGA33_001597"
     CDS             1341..1457
                     /locus_tag="PGA33_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473405.1"
                     /translation="MLQLFLMKIALNDFENDKSVLFIFLKNHFYEEMCVLSN"
     gene            1602..2246
                     /locus_tag="PGA33_001598"
     CDS             1602..2246
                     /locus_tag="PGA33_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDG6473406.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            2362..3228
                     /locus_tag="PGA33_001599"
     CDS             2362..3228
                     /locus_tag="PGA33_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDG6473407.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKEWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
     gene            3498..4718
                     /locus_tag="PGA33_001600"
     CDS             3498..4718
                     /locus_tag="PGA33_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDG6473408.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            4715..5203
                     /locus_tag="PGA33_001601"
     CDS             4715..5203
                     /locus_tag="PGA33_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HDG6473409.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(5302..5991)
                     /locus_tag="PGA33_001602"
     CDS             complement(5302..5991)
                     /locus_tag="PGA33_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HDG6473410.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
     gene            6118..6561
                     /locus_tag="PGA33_001603"
     CDS             6118..6561
                     /locus_tag="PGA33_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6473411.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQVESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISNTIHKNNLPMISLNKDLGYQVSHVKMYKDID"
     gene            6628..7023
                     /locus_tag="PGA33_001604"
     CDS             6628..7023
                     /locus_tag="PGA33_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473412.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKEASQDKQSK
                     DQTKSSDKDNHKKPTSADKDQKANDKHQS"
     gene            7161..7460
                     /locus_tag="PGA33_001605"
     CDS             7161..7460
                     /locus_tag="PGA33_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473413.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGHTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            7540..8082
                     /locus_tag="PGA33_001606"
     CDS             7540..8082
                     /locus_tag="PGA33_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6473414.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(8178..8744)
                     /locus_tag="PGA33_001607"
     CDS             complement(8178..8744)
                     /locus_tag="PGA33_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDG6473415.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(8746..9204)
                     /locus_tag="PGA33_001608"
     CDS             complement(8746..9204)
                     /locus_tag="PGA33_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDG6473416.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            complement(9207..9890)
                     /locus_tag="PGA33_001609"
     CDS             complement(9207..9890)
                     /locus_tag="PGA33_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473417.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
BASE COUNT         3448 a         1373 c         1748 g         3337 t
ORIGIN      
        1 taaaaaaatc atgtataata tatgttgtta attaaacagt tcgaaagcga gatgacatta
       61 tgggacgtaa atggaataac attaaagaaa aaaaggccca aaaagataaa aacacaagta
      121 gaatatatgc gaaatttggt aaggagattt atgttgcagc aaaatctggt gaacccaatc
      181 cagaatctaa ccaagcttta aggttggtgc ttgaacgcgc taagacatat tcagtgccga
      241 atcatattat tgaaaaagca atagataaag ctaagggtgc tggagacgaa aactttgatc
      301 acctaagata tgaaggattt ggcccaagcg gatcaatgct aattgttgat gcgttaacaa
      361 ataatgtaaa tcgtactgcc tctgatgtgc gagctgcttt tggtaaaaac ggcggtaata
      421 tgggtgtatc tggatcagtt gcttatatgt ttgatcatgt ggcaacattt ggtattgaag
      481 gaaagtctgt tgacgaaata cttgaaacat taatggaaca agatgtagat gtaaatgatg
      541 tgattgacga taatggattg acaatagtct atgctgaacc agatcaattt gcagtcgttc
      601 aagatgcgct tcgtgcagca ggtgttgaag aatttaaagt tgctgaattt gaaatgttac
      661 ctcaaacaga tattgaactt tctgaagcgg accaagtaac atttgaaaaa ttaatcgatg
      721 cattagaaga tttagaagat gtacaaaacg tattccataa tgtggatttg aaataatgaa
      781 atcagcagaa caatggattg atgaattgca acttgaatca catcctgaag gtggtttcta
      841 tagagagaca attcgagaag tattgaaaga tggacgcaga gcgccgttta gtagtattta
      901 ttttttactt acagatgaca atatttcgca ttttcatcga attgatgctg atgaagtatg
      961 gtactatcat gctggcgatt ctctaacaat tcatatgata aatccggatg gggaatatac
     1021 gactgcaaca ttgggtactg atatccaaaa tggagatgta ttgcaatatg tagtgcctaa
     1081 aggaacaatt tttgcttctt caatcgaaat atcaaatact tttagtttag taggttgtat
     1141 gtgtcaaccg gcatttgagt ttaagcagtt tgaattgttt aagcaatctg aattaattac
     1201 gcaatatccg catcttaaat cagtaatcga aaaatatgct ttaaaataaa agtgatcaat
     1261 gaagtggttt gaaggttgtt aataaacctt tgagtcactt catttttata tgtattcttg
     1321 attgaatcag aatagatttg atgcttcagc tgtttttaat gaaaatagca ttaaatgatt
     1381 ttgaaaacga taagagtgtg ttatttatat ttttgaaaaa tcacttttat gaagaaatgt
     1441 gtgtactatc taattaatat tgtctatttt aagtaaattg aagcgagttg tagtaaaata
     1501 ataataaata ttttcatatg attaacaaaa actataaaac tgtatcatga catgacatca
     1561 tttatgtagt tttatataat aatgacaagg agttggtgac tgtgaaagta aagtatatag
     1621 ataaacgtca ctggcgtcgc ctaattgata gggaatacac agaggtaaaa gttaataata
     1681 ataggtttaa gggtattata ggcttagtca cgatgaaaaa ggttcgtgat cctttagagg
     1741 tgacggtagt tggacaaaat atcattgtcg cagatgacaa ttataaatgg ttgcaaatac
     1801 tacctgaaaa gaaacgttat agtataactg taatgtttga taataaaggc aatccattag
     1861 aatattattt tgatataaat atcaaaaata taacgcaaaa aggtaatgcg cgtacagtag
     1921 atttatgttt agatgtttta gcattaccaa gtggtgaata tgagttggta gatgaagatg
     1981 atttaatgtt tgcattagaa agtgagcaaa ttacaaaaaa gcaatttcat gaagcatata
     2041 tgattgcaca tcaaattatg gcagagttag aaaatgattt taaaggattc caaaagaaaa
     2101 tcatgtactg ctttaataaa attaatgcaa aggctcaaaa aaatcatcaa aagccacaaa
     2161 ataaaactaa tattgaaaaa agcaaacaaa taaagcctaa gcaatataat caaactaaaa
     2221 atcaccaaca acaaaagaaa aactaagtaa ttcaagctgc agccatacca ataaaattgg
     2281 tgaaattatt cgttagtaat gaactcatga atgcttgtag ccatgaaagt tcaataattg
     2341 aataatttat ggggggaaat tatgaagatt gaagactatc gtttactaat aacattagac
     2401 gaaacgaaaa cgttacgtaa agcggctgaa attttatata tatctcaacc tgctgttaca
     2461 caaagactaa aagctattga aaatgctttt ggagtagata tttttatcag aacaaaaaaa
     2521 caattgatta caacaactga aggaacaatg attattgagc atgctcgtga catgttgaaa
     2581 agagagcgat tattttttga caaaatgcag gcacatattg gtgaagtgaa tggaacaata
     2641 tcaatcgggt gttcttcttt gattggacaa accttacttc ctgaagtttt gagcctatat
     2701 aatgcccaat ttcctaatgt tgaaatacaa gtgcaagttg gttcaactga acaaattaaa
     2761 gcaaatcata gagattatca tgttatgata actcgtggaa ataaggtaat gaatttagct
     2821 aacacacatt tatttaatga tgatcattat tttatttttc caaaaaatag acgagatgat
     2881 gttacaaagt taccatttat agagtttcaa gctgatccga tttatataaa tcaaataaaa
     2941 gaatggtata acgataattt agaacaagat taccatgcaa ctattacagt ggatcaagta
     3001 gcaacttgca aagaaatgtt gattagtggt gtaggtgtta caatcttgcc agaaattatg
     3061 atgaaaaata tcagcaaaga acaatttgag tttgaaaaag tagaaattga taatgaaccg
     3121 ctgattcgtt cgacatttat gagttatgat ccgagcatgt tgcaattgcc acaagttgat
     3181 tcttttgtaa atctcatggc gagctttgtt gaacaaccaa aggcgtagtt ttagactaat
     3241 ttaaggtttg tatttaattt taaactattc ggttaaattg aacgtagttg gttgctaatg
     3301 caccaacagc aaaagagccc ctaattaata aattaaaagg ggacaaagga atacagttgg
     3361 ttgctaatgc accaactgca taagagcccc taattaataa attaaaaggg gacaaaggaa
     3421 tacagttgga tgctaacgca ccaactgcat aagagcccct aattaataaa ttaaaagggg
     3481 ctctaagaag cggggttatg gatatgtttg cagcgttatt acaaataaag aattataaac
     3541 tctttgttgc taatatgttt ctactaggta tgggtattgc ggttacggtc ccatatcttg
     3601 ttctttttgc aactaaagat ttaggtatga caacaaatca gtatggatta cttctagcat
     3661 ctgcagcgat tagccagttt acagtaaatt caattattgc tagattttcg gatacgcatc
     3721 actttaatag aaaaattatt attattctcg cattattaat gggtgcgctt ggtttttcaa
     3781 tatacttttt tgtagataca atctggttat tcatattact atatgcgatt ttccaaggat
     3841 tatttgcacc agcaatgccc caactttacg catctgctag agaatctatc aatgtttcaa
     3901 gctctaaaga tagagctcaa tttgccaaca cagtattacg ttcaatgttc tcattgggct
     3961 ttttatttgg tccatttatt ggtgcccaat taatcggatt aaaaggctat gctggattgt
     4021 ttggtggaac aataagtatc attttattta ctttagtact tcaagtgttt ttctataagg
     4081 atttaaacat taaacaccct attagtacgc aacaacatgt tgaaaaaatt gctcctaata
     4141 tgtttaaaga caaaacgctt ttattaccat ttattgcatt tattttatta cacattggac
     4201 aatggatgta tacgatgaat atgcctttat ttgttactga ttatttaaaa gaaaatgaac
     4261 aacatgtcgg ttatttagct agtttatgtg ctggtttaga agtgccattt atgatcattc
     4321 ttggcgtttt atcatctaga ttacagactc gaacattgtt gatttatgga gcgatttttg
     4381 gtggtttatt ctacttcagc attggggtat ttaaaaactt ctatatgatg ttagcaggac
     4441 aggtgttttt agctattttc ttagcggttc ttttaggaat tggtattagt tatttccaag
     4501 atatcttacc agattttcca ggatacgctt caacactatt ttctaatgca atggttattg
     4561 gacagttagg cggtaaccta ttaggtggtg ctatgagtca ctgggtaggt ttggaaaatg
     4621 tattttttgt atcagcagca tcaatcatgt taggtatgat acttatattc tttactaaaa
     4681 atcaaaaaat tacaaaagag gatgtgatat caacatgaca attattttat ggctacttat
     4741 catcgctgcc ttcatgttag catttgttgg gttgattaag ccgattattc cttctgtttt
     4801 agtattatgg gttggctttt taatctatca atttggcttt cataatcagc atttatcatg
     4861 ggtgttttat gtatctatgg cattgctaac aatattaatt ttatgtgccg actttttagc
     4921 taataaatat tttgtgaatc gcttcggtgg ttctaagttt ggagagtatg cagctttaat
     4981 tggtgtggtt attggatgtt ttgttttacc gccatttgga attattatta taccttttat
     5041 tttggtattc atagttgaat taatacaagg ctattcattt gaaagagcag ttaaagtaag
     5101 tataggttca atcgtagcat ttttaacaag tagtatagct caagcaatca ttatgtttat
     5161 aatgattgta tggttcttta tagatgcttt attgattaat taataaaaag cttattgcaa
     5221 aatatgtttt tcggtaactg taatttagtg attttatcat taacagtacc aaattcgcat
     5281 atgatgtaat aagctttttg tttataaaaa tgtatgagaa tgttttttcg aaatatttct
     5341 ttcaatgcgt aatccaatag gcataactat aaatgaaaat ataataaata cccaattgct
     5401 tacctgaata tatggtccaa aggcaagtaa tgattgtgga ataaagaata catagaaaaa
     5461 tcctacaatt agtagaatga ttgctaaata ggtgaatatc catatccaat ttgaattgtt
     5521 aaaacactgc agttgtattg ttttaaaact aaaccatata aataaaaaga caataaagaa
     5581 tccaacgact actgaaaacg ggaatgaaac aaaatataaa ttacttccat ttttttccat
     5641 gaaaaatcct aaaaatcctt tgagaaaact aacaatccca attaatagaa cgatgtgttg
     5701 atagatatat ttaaaaatat ttttaaatgt ttcattaggc atcgctttta gttcttttat
     5761 tgcatgtgct tttgggtcgt gattgaaaaa atctaaggct aataaaccat gttgttctgc
     5821 gcttaataat tgtttgagta tacggttaat aattaactct gtatcatgag ggttgacgcg
     5881 aaagtcagag cgcatataag tcatataatt ctcgaagatt tctctatcag tattgcttaa
     5941 tcttaatgat ttaacattat tttcttttgt taattgcgca gtacttttca ttgttactta
     6001 agcgctcctt taaaaatgtt taattccaaa ttaaaatgga aatgatttta tagtattaat
     6061 aaggtcaatc atatcatatt aaacgcataa atataacgat taatattgga gaggaaaatg
     6121 aggacactta ataaagatga acataattat atcaagcaaa tagctaatat acatgagaca
     6181 ttattgtcgc aagtagaatc caactataaa tgtactaaac tgagtattgc tcttaggtac
     6241 gagatgatat gttcaagatt agaacataca aatgataaaa tttatatata tgaaaatgaa
     6301 ggtcaattaa tagcgtttat ttggggacat tttagtaatg aaaaaagtat ggttaacatt
     6361 gaactgctat atgttgaacc acaatttcgc aaactgggaa tagctacgca actgaagatt
     6421 gcgcttgaaa aatgggcaaa aactatgaat gcaaagcgaa taagcaatac aattcataaa
     6481 aataatttgc caatgatatc tttgaataaa gatttaggtt atcaagtgag tcatgtgaaa
     6541 atgtataaag atattgatta gaattaggat tatgttgcta attcatgtta aaattaaaaa
     6601 agatttaatg acgttaagga gttttatatg aagaaattaa tcatcagtat tatggcggtc
     6661 atgctatttt taacaggttg tggtaaaagt caagagaaag ccactctgga aaaggatatc
     6721 gataatttac aaaaagaaaa taaagaatta aaagacaaaa aagaaaagct tcaacaagaa
     6781 aaagaaaaat tagcagataa gcaaaaagac cttgaaaaag aagtgaaaga tttaaaacct
     6841 tcaaaagaag ataacaagga tgataaaaaa gacgaagaca aaaataaaga caaagataaa
     6901 gataaagagg catcacaaga taagcaatca aaagatcaaa ctaagtcatc ggataaagat
     6961 aatcacaaaa agcctacatc agcagataaa gatcaaaaag ctaatgacaa acaccaatca
     7021 taatcgaatt gcttacttgt tatagatgaa aggtacagcg ttttaaacct tattttaagg
     7081 gtatgtatta attaaaatgt ggtcatgatt gaaacagaac gtaaaaatag acaacgtaat
     7141 taataaagga gagaaacggc atgcatgaac aagattttag aattttagag ggtcaagata
     7201 ttactttgcc agaattaggt agagaattag aaaatattac aggacatacg attgctgatt
     7261 ctactggcga aattaagcgt gtaattgcac atttaccaaa ctttgagtcc gatacagata
     7321 cttttgttgc tacatatcgt ttaaaccatc aacaagattt tatagatgca acttttactg
     7381 cgctgaaatc agatagagca cgtttaaaag aagtgccagt tcatgttgaa cttataagtt
     7441 atatttctaa atcaaaataa actgctatct aaaacgcaaa gttgatcaaa atatcgattt
     7501 tgtgtttttt attgagaaat tatataggag tgtcaatcga tgatttattg tgaaacagag
     7561 cgtttaatat taagagactg gcatgaagat gatctgttac cttttcaaaa aatgaatgcg
     7621 aattatgacg tacgtaaata ttttccaagt ttattgagtt atcgtcgttc agaattagat
     7681 atgagaacta tggatgcggt tattaaagat tatggcattg gattatttgc tgtagaagat
     7741 aaagagtccc atcaatggat aggctttata ggtttgaatt atattccaga aacaagcgat
     7801 tatccattta aagaattacc gctttatgaa ataggttggc gcttgttgcc agaattttgg
     7861 ggaaaaggat tagcaactga aggcgcaaag gcaacattga agttagcaga agaacatcaa
     7921 atatacgatg tctatagttt tacagcagaa gcaaataaag cttcacaacg tgtaatggaa
     7981 aaaattggca tgacagtgta tgatcatttc gaattaccca atctaagtaa gtatcattta
     8041 ttaaaaaggc aagtgcgcta ttacattaat cttcgaaagt gaaaaattta tacataagcg
     8101 taacaaacac ccctaacatt gtttagctga tgataacaat attaggggtg tttgtttatt
     8161 ttttaacctt agaatgatta atcgtatgaa cgagtaccca gaggtttgaa atttaatatt
     8221 gattcaatta atgattcctt agtgtcgcat aacggtgcaa gagcacgata cttaggatca
     8281 ataaaacctt cctcaatcat atggtcaatc attgtttgta gtggattgaa aaagccatta
     8341 atattataaa tggcaatagg cttttcatgg atacctattt gagcccaact atacatttcg
     8401 aaaaattctt ctagtgaacc tgcgccacca ggagccatga caaatgcatc tgcaagttct
     8461 gccattttat ttttacgttc atgcatagaa tcaactaaaa ttaattcagt taaacgttgg
     8521 cttgtgattt catgttcatc taacatttta ggcatgacgc caatagcttt gccgccatga
     8581 tctaatacac catcttgaat ggcacccata atgccaattg accctgcacc aaatactaat
     8641 tcataacctt gttcagcaaa atatttacct aaatcgtatg ctttttgtac atatgaaggg
     8701 tcatgacctt tgcttgcacc acaataaact gcgattcgtt tcatgttaat ccagctcctt
     8761 aattcgatga atgactttta atagtgattg ttcaaacact ttttgatctt gctttgtaaa
     8821 aggtggggga cctttgtggc gaccaccttg ttttctaatt tgtgcattca tatatcgttt
     8881 atctaatagt tgttgaatat ttttggaatt gtatatcttc ccattatgat gcatgacaat
     8941 taagactttg tcgactaata aacttgcgag tccataatct tgagtgacta cgatatcatc
     9001 cttcgttgat aattgaacaa ttttgtaatc aactgcatct ggtccatcat caacatataa
     9061 tgttgataca tgtggaggat ataattggtt cgaaaaatgg ctgaagctcc gaataattgt
     9121 cacaaaaatg cctgtctcag ttgttaaatc tataatagaa tcaacaacag gacaagcatc
     9181 tccatcaata ataatatgtg tcacaattat gcctctgtat tgttttcttt attttgttga
     9241 gaggcgcttt tggcaacata atctttatat tttttaaatg acttgatgcg tgctttatca
     9301 gcttcttgtt ggcgtttttg ttcttctttg tgtcgttttt caatattttt ttgtaacttt
     9361 ttattcattt tagcgatttc tttgcgattt ttttcagcta gtttatcgcc ttttttctca
     9421 gttttctcat ctaatttatt aggtgttaag cctgcttttt cttcgtattt ttgtgatttt
     9481 ttcatatctt taatacgttg tatttcattc ttttcgcggg ctttttgctc ttctttatga
     9541 cgcttttcga tatttttttg aagtatttta ttcattttat cagcgtcttt acgattttgt
     9601 ttagctaatt tttcgccttt tttctcaata taggcaggat catgttctct agcaaacttt
     9661 ttaagttcac gtttattttc aaaatcttgt tttttatcgc cgacatattc tttaacatca
     9721 ctcgctgtgt tactgattgc tgcagatgtt tttgaagcaa ctttacttgt agcatctgta
     9781 actttttgta cgtccggatg ttgtttgata cgtttacgtt caacaattaa cggtaccaat
     9841 acaattggta atacattaat cataaatttg atgacttttt tcttatccat agatcttgcc
     9901 tccata
//
LOCUS       DAMQYT010000088         9902 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMQYT010000088 DAMQYT010000000
VERSION     DAMQYT010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9902
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.088"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            2..610
                     /locus_tag="PGA33_001610"
     CDS             2..610
                     /locus_tag="PGA33_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HDG6473418.1"
                     /translation="MAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPIKTVGKK
                     IAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSEQDFKKA
                     KSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLNQNDATQ
                     QGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            781..1893
                     /gene="pdhA"
                     /locus_tag="PGA33_001611"
     CDS             781..1893
                     /gene="pdhA"
                     /locus_tag="PGA33_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDG6473419.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            1897..2874
                     /locus_tag="PGA33_001612"
     CDS             1897..2874
                     /locus_tag="PGA33_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDG6473420.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            2965..4257
                     /locus_tag="PGA33_001613"
     CDS             2965..4257
                     /locus_tag="PGA33_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDG6473421.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            4261..5667
                     /gene="lpdA"
                     /locus_tag="PGA33_001614"
     CDS             4261..5667
                     /gene="lpdA"
                     /locus_tag="PGA33_001614"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDG6473422.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNGIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            5835..6110
                     /locus_tag="PGA33_001615"
     CDS             5835..6110
                     /locus_tag="PGA33_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDG6473423.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            6254..6793
                     /locus_tag="PGA33_001616"
     CDS             6254..6793
                     /locus_tag="PGA33_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDG6473424.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            6806..7900
                     /locus_tag="PGA33_001617"
     CDS             6806..7900
                     /locus_tag="PGA33_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6473425.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            7893..8690
                     /locus_tag="PGA33_001618"
     CDS             7893..8690
                     /locus_tag="PGA33_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6473426.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAASITIITLTISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            8696..9505
                     /locus_tag="PGA33_001619"
     CDS             8696..9505
                     /locus_tag="PGA33_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6473427.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            9505..>9902
                     /locus_tag="PGA33_001620"
     CDS             9505..>9902
                     /locus_tag="PGA33_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDG6473428.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDRGNKYS"
BASE COUNT         3571 a         1419 c         1890 g         3022 t
ORIGIN      
        1 aatcgcagta gtattagcat ctagtgtctt gcttgcagga tgtactacgg ataaaaaaga
       61 aattaaggca tatttaaagc aagtggataa aattaaagat gatgaagaac caattaaaac
      121 tgttggtaag aaaattgctg aattagatga gaaaaagaaa aaattaactg aagatgtcaa
      181 tagtaaagat acagcagttc gcggtaaagc agtaaaggat ttaattaaaa atgccgatga
      241 tcgtctaaag gaatttgaaa aagaagaaga cgcaattaag aagtctgaac aagactttaa
      301 gaaagcaaaa agtcacgttg ataacattga taatgatgtt aaacgtaaag aagtaaaaca
      361 attagatgat gtattaaaag aaaaatataa gttacacagt gattacgcga aagcatataa
      421 aaaggctgta aactcagaga aaacattatt taaatattta aatcaaaatg acgcgacaca
      481 acaaggtgtt aacgaaaaat caaaagcaat agaacagaac tataaaaagt taaaagaagt
      541 atcagataag tatacaaaag tactaaataa ggttggtaaa gaaaagcaag acgttgatca
      601 atttaaataa ttaatataat acagatggta ggaaacaact aatacagttc ctattatctg
      661 tatctttttt tattaaaaca gaactttttc aaatggttta acagtcccat ttatttgtgg
      721 tacaattagt aaggataaaa tgaatttcta tacaattatg ggaaaggtat ggtgaattga
      781 atggctccta agttacaagc ccaattcgat gcagtaaaag ttttaaatga tactcaatcg
      841 aaatttgaaa tggttcaaat tttggatgag aatggtaacg tcgtaaatga agacttagta
      901 cctgatctta cggatgaaca attagtggaa ttaatggaaa gaatggtatg gactcgtatc
      961 cttgatcaac gttctatctc attaaacaga caaggacgtt taggtttcta tgcaccaact
     1021 gctggtcaag aagcatcaca attagcgtca caatacgctt tagaaaaaga agattacatt
     1081 ttaccgggat acagagatgt tcctcaaatt atttggcatg gtttaccatt aactgaagct
     1141 ttcttattct caagaggtca cttcaaagga aatcaattcc ctgaaggcgt taatgcatta
     1201 agcccacaaa ttattatcgg tgcacaatac attcaagctg ctggtgttgc atttgcactt
     1261 aaaaaacgtg gtaaaaatgc agttgcaatc acttacactg gtgacggtgg ttcttcacaa
     1321 ggtgatttct acgaaggtat taactttgca gcagcttata aagcacctgc aattttcgtt
     1381 attcaaaaca ataactatgc aatttcaaca ccaagaagca agcaaactgc tgctgaaaca
     1441 ttagctcaaa aagcaattgc tgtaggtatt cctggtatcc aagttgatgg tatggatgcg
     1501 ttagctgtat atcaagcaac taaagaagca cgtgaccgcg cagttgcagg tgaaggtcca
     1561 acattaattg aaactatgac atatcgttat ggtcctcata caatggctgg tgacgatcca
     1621 actcgttaca gaacttcaga cgaagatgct gaatgggaga aaaaagaccc attagtacgt
     1681 ttccgtaaat tccttgaaaa caaaggttta tggaatgaag acaaagaaaa tgaagttatt
     1741 gaacgtgcaa aagctgatat taaagcagca attaaagagg ctgataacac tgaaaaacaa
     1801 actgttactt ctctaatgga aattatgtat gaagatatgc ctcaaaactt agcagaacaa
     1861 tatgaaattt acaaagagaa ggagtcgaag taagccatgg cacaaatgac aatggttcaa
     1921 gcgattaatg atgcgcttaa aactgaactt aaaaatgacc aagatgtttt aatttttggt
     1981 gaagacgttg gtgttaacgg cggtgttttc cgtgttactg aaggactaca aaaagaattt
     2041 ggtgaagata gagtattcga tacaccttta gctgaatcag gtattggtgg tttagcgatg
     2101 ggtcttgcag ttgaaggatt ccgtccggtt atggaagtac aattcttagg tttcgtattc
     2161 gaagtatttg atgcgattgc tggacaaatt gcacgtactc gtttccgttc aggcggtact
     2221 aaaactgcac ctgtaacaat tcgtagccca tttggtggtg gcgtacacac accagaatta
     2281 cacgcagata acttagaagg tattttagct caatctccag gtctaaaggt tgttattcct
     2341 tcaggcccat acgatgcgaa aggtttatta atttcttcta ttagaagtaa tgacccagtc
     2401 gtatacttag agcatatgaa attgtatcgt tcattccgtg aagaagtacc tgaagaagaa
     2461 tatacaattg acattggtaa ggctaatgtg aaaaaagaag gtaatgacat ttcaatcatc
     2521 acatacggtg cgatggttca agaatcaatg aaagctgcag aagaacttga aaaagatggt
     2581 tattctgttg aagtaattga cttacgtact gttcaaccaa tcgatgttga cacaattgta
     2641 gcttcagttg aaaaaactgg tcgtgcagtt gtagttcaag aagcacaacg tcaagctggt
     2701 gttggtgcag cagttgtagc tgaattaagt gaacgtgcaa tcctttcatt agaagcacct
     2761 attggaagag ttgcagcagc agatacaatt tatccattca ctcaagctga aaatgtttgg
     2821 ttaccaaaca aaaatgacat catcgaaaaa gcaaaagaaa ctttagaatt ttaatacatt
     2881 ttaaaagtta acgaagttag cgtattttag tctcattgat taaaatgaaa tgtttaattt
     2941 acgaaatctt aggagggcaa aaacgtggca tttgaattta gattacccga tatcggggaa
     3001 ggtatccacg aaggtgaaat tgtaaaatgg tttgttaaag ctggagatac tattgaagaa
     3061 gacgatgttt tagctgaggt acaaaacgat aaatcagtag tagaaatccc atcaccagta
     3121 tctggtactg tagaagaagt tatggtagaa gaaggtacag tagctgtagt tggtgacgtt
     3181 attgttaaaa tcgatgcacc tgatgcagaa gatatgcaat ttaaaggtca tgatgatgat
     3241 tcatcatcta aagaagaacc tgcgaaagag gaagcgccag cagagcaagc acctgtagct
     3301 actcaaactg aagaagtaga tgaaaacaga actgttaaag caatgccttc agtacgtaaa
     3361 tacgcacgtg aaaaaggtgt taacattaaa gcagtttctg gatctggtaa aaatggtcgt
     3421 attacaaaag aagatgtaga tgcatactta aatggtggtg caccaacagc ttcaaatgaa
     3481 tcagctgctt cagctacaag tgaagaagtt gctgaaactc ctgcagcacc tgcagcagta
     3541 acattagaag gcgacttccc agaaacaact gaaaaaatcc ctgctatgcg tagagcaatt
     3601 gcgaaagcaa tggttaactc taagcatact gcacctcatg taacattaat ggatgaaatt
     3661 gatgttcaag cattatggga tcaccgtaag aaatttaaag aaatcgcagc tgaacaaggt
     3721 actaagttaa cattcttacc ttatgttgtt aaagcacttg tttctgcatt gaaaaaatac
     3781 ccagcactta acacttcatt caatgaagaa gctggtgaaa tcgttcataa acattactgg
     3841 aatatcggta ttgcagcaga cactgataga ggattattag tacctgttgt taaacatgct
     3901 gatcgtaagt ctattttcca aatttcagat gaaattaatg aattagctgt taaagcacgt
     3961 gatggtaaat taacagccga tgaaatgaaa ggtgctacat gcacaatcag taatatcggt
     4021 tcagctggtg gacaatggtt cactccagtt atcaatcacc cagaagtagc aatcttagga
     4081 attggccgta ttgctcaaaa acctatcgtt aaagatggag aaattgttgc agcaccagta
     4141 ttagcattat cattaagctt tgaccacaga caaattgatg gtgcaactgg ccaaaatgca
     4201 atgaatcaca ttaaacgttt attaaataat ccagaattat tattaatgga ggggtaaaac
     4261 atggtagttg gagatttccc aattgaaaca gatactatag taatcggagc aggtcctggt
     4321 ggatacgttg cagcaattcg tgcagctcaa ttaggacaaa aagtaacaat cgttgagaaa
     4381 ggtaatcttg gtggtgtttg cttaaacgta ggatgtattc cttcaaaagc attactacat
     4441 gcttctcacc gttttgttga agcacaacat tctgaaaact taggtgttat tgctgaaagt
     4501 gtttctttaa acttccaaaa agttcaagaa ttcaaatcat cagttgttaa taaattaact
     4561 ggtggtgttg aaggcttact taaaggtaac aaagttaaca tcgttaaagg tgaagcatat
     4621 ttcgtagata acaatagctt acgtgttatg gacgaaaaga gcgcacaaac atacaacttt
     4681 aaaaatggaa tcattgcaac aggttcaaga ccaattgaaa ttcctaattt caaattcggt
     4741 aaacgtgtta tcgactcaac aggtgcttta aacttacaag aagtaccagg taaattagtt
     4801 gtagttggtg gaggatacat tggatcagaa ttaggtacag catttgctaa ctttggttca
     4861 gaagtaacca tccttgaagg tgctaaagat atcttaggtg gcttcgaaaa acaaatgaca
     4921 caacctgtta aaaaaggtat gaaagaaaaa ggtgttgaaa tcgttactga agctatggct
     4981 aaatcagctg aagaaacaga taacggagtt aaagttactt atgaagctaa aggcgaagag
     5041 aaaacaatcg aagctgatta tgtattagta actgtaggtc gtcgtccaaa cacagacgaa
     5101 ttaggcctag aagaattagg tgttaaattc gctgaccgtg gattattaga agttgataaa
     5161 caaagccgta cgtctatcag caatatctat gcaattggtg atatcgttcc aggtttacca
     5221 cttgctcaca aagctagcta tgaagctaaa gttgctgctg aagcaattga tggtcaagct
     5281 gctgaagttg attacattgg tatgccagca gtatgcttta ctgaaccaga attagctaca
     5341 gttggttatt cagaagcgca agctaaagaa gaaggtttag caattaaagc ttctaaattc
     5401 ccatatgcag caaatggtcg tgcattatca ttagatgata ctaacggatt tgttaaactt
     5461 attacactta aagaagatga tactttaatc ggtgctcaag tagttggtac tggtgcatca
     5521 gatattatct ctgaattagg tttagcaatt gaagctggta tgaatgctga agatatcgca
     5581 ttaacaatcc atgcacatcc aacattaggt gagatgacta tggaagcagc agaaaaagct
     5641 atcggatacc caatccatac aatgtaataa ctgattatct ataaagattc agtcattaaa
     5701 agctgtagca tatgctacgg cttttttgtt ttaggtaaag taatgtaagg aaattgattt
     5761 gagatatcgt taacatgtga catgcatgtt atactagcga tgctaacaaa agaattgaaa
     5821 tggagggttc aacaatggaa tatgagtatc caattgattt agactggagt aatgaagaga
     5881 tgatttcagt gataaatttc tttaatcatg tagagaagta ttatgaatcc ggcgtgacgg
     5941 caggcgactt tatgggtgca tataaaagat ttaaagaaat tgtgcctgct aaagcagagg
     6001 aaaaacaaat ttttaatact ttcgaaaaaa gtagtggcta taatagttac aaagcagttc
     6061 aagatgtaaa aactcactct gaagaacaaa gagtaacagc taaaaaataa ttcgttcgaa
     6121 attaacacaa tttaatagga atttttcttt aaaactattg ctaataaagc tatattttga
     6181 tacctttatc aagtgttaaa caaaatgttt gataaaagta aacttaatat agctttttta
     6241 ggtggaaaaa taaatgaaca taggtaataa aattaaaaat cttagaagaa ttaaaaattt
     6301 aacgcaagaa gaacttgctg aacgtacaga cttatcgaaa ggctacattt cacaaataga
     6361 aagtgaacat gcctcaccaa gtatggaaac tttcttaaat attatagagg tgttaggaac
     6421 gacgccaagt gaatttttta aagacagtga aaatgaaaaa gtattataca agaaggaaga
     6481 acaagttatt tatgatgagt atgatgaagg ttatatatta aattggttag tttcaaagtc
     6541 aaatgaatat gatatggagc cattaatatt aactttaaag cctggagcat catataaaaa
     6601 ttttaatcca tcagagtctg atacgtttat ttattgtatg tcaggtcaga taacacttaa
     6661 tttaggcaaa gagatatatc aagcacaaga agaagacgtt ttgtatttta aagcacgaga
     6721 taatcatcgt ttgtcaaacg aatcaaacaa tgaaacacga atacttattg tagcgacagc
     6781 ttcatattta taggggggat cttatttgga accgttatta tcattaaaat cagttagtaa
     6841 aagctatgat gatcttaata tcttagatga catagatatt gatattgaat caggatactt
     6901 ttatacatta ttaggtcctt caggttgtgg taaaacaaca attttaaaat taattgcagg
     6961 gtttgaatat cctgacagtg gtgaagtgat ttatcaaaac aaaccaattg gtaatttacc
     7021 accaaataaa cgtaaagtga atacagtctt tcaagattat gcattatttc cacacttaaa
     7081 cgtctatgat aatatcgctt ttggtttgaa attaaaaaaa ttatcaaaaa ccgaaattga
     7141 tcaaaaagta actgaggcat taaaattagt aaaactttca ggttatgaaa aaagaaatat
     7201 taatgaaatg agtggcggac aaaagcaacg tgttgcaatt gcacgtgcta tcgtaaatga
     7261 accagaaata ttattgttag atgaatcttt atccgcatta gatttgaaat tgcgtactga
     7321 aatgcaatat gaattacgag aattgcaatc tagattaggt attacattta tatttgtaac
     7381 acatgatcaa gaagaagcgt tagcattaag tgactttctt tttgtattaa aagatgggaa
     7441 aattcaacaa tttggcacac caacagatat atatgacgaa ccagtgaata gatttgtagc
     7501 tgattttatt ggagaatcta atattgttga agggcgcatg gttagagatt atgtcgtgaa
     7561 tatttatggg caagatttcg aatgtgtcga tatgggtatt cctgaaaata aaaaagtaga
     7621 agtcgttatt cgaccagaag atatatcatt aatcaaagct gaagaaggat tatttaaagc
     7681 aactgttgat tctatgttat ttagaggggt ccactatgaa atatgttgta tagacaataa
     7741 aggttatgaa tgggtaatac aaacgactaa aaaagctgaa gtaggcagtg aagttggtct
     7801 ttattttgat cctgaagcca ttcatattat ggttcctgga gaaacagaag aagaatttga
     7861 taaacgtatt gaaagctatg aggaagtaga caatgcgtaa tactaataaa tttctcttaa
     7921 tcccgtattt actatggatg gttatattta ttattgtacc agttgtatta ctcatttatt
     7981 tttcattttt agatatcaat ggacatttta gtttcacgaa ttatcaacaa atttttacta
     8041 caaaatattt gaaaatgttt gcatattcaa ttttatatgc cgcttcaata acaattatta
     8101 ccttgactat cagttatcca gctgcctatt atattactcg ttcgaaattt caaaatatct
     8161 tattaatgat aatgattatt ccaacatgga taaatttatt gttaaagaca tatgctttta
     8221 taggtttatt aagtcatgat ggcgtgatta atcaattttt ccacttattt aatttaccat
     8281 cattcaattt gttatttaca actggtgcgt ttttagtagt ggcaagttac atttatatac
     8341 catttatgat tttacctata tttaatagca tgaaagcaat tcctaataat ttattgcagg
     8401 cctcaagtga tttaggtgcc agtcctttct atacttttag aaaagtaatc atgccgttaa
     8461 caaaagaagg tgttatgact gggattcaag taacctttat tccatcactt tcattattta
     8521 tgattacaag attaattgca ggtaataaag tcataaatat aggtacggca atagaggaac
     8581 aatttttaac aattcaaaat tatggtatgg gatcaactat agctatattc ttaattgtat
     8641 ttatggcatt cattttaatc attacaaaat catctaatgg gagagggtga atcatatgaa
     8701 atggtatgga aagctgtata tcgggatact tttagcgatt ttatacatcc caatattctt
     8761 tttaatgttc tattcattca attcggctgg taatatgatt cactttgaac attttacatt
     8821 agagcattat caatcattat ttcaaaatga tcatttaatg tcggtcattt ttaatacgat
     8881 agctgtagca cttttagcag cctcaatttc tacagttatt ggtacatttg gtgccattgc
     8941 tatttattat ttaagaaata aaaagtttaa agtaacttta ctaacattga ataatgtctt
     9001 gatggtatca tccgacgttg tcataggtgc atcattctta attatgttta caacgattgg
     9061 ccattttact ggtcttggtt taggattttg gacagttcta atatctcata ttgcattttg
     9121 catacctata gttgtgatta tcgtcttacc acaactgtat gaaatgaata ataatatgtt
     9181 aaatgctgca agagatttag gagcgactga accacaatta ttaagcaaca ttattattcc
     9241 taatatttta ccttctatta taggaggatt ctttatggct ttaacttatt cactagacga
     9301 tttcacagta agtttcttcg ttactggtaa tggctttagt gtgttatcag ttgaagttta
     9361 tgctatggcg agaaaaggaa ttagtatgga aattaatgcg atttcaacat tattgtttgc
     9421 tgttattgta ttaggaatac taggatatta tttgattcaa tacgtgataa ataagaaaaa
     9481 actaatcaag cgaggtgtaa aataatgaaa cgatttttac aactcattat aggtgcatta
     9541 gttgtgggta tgctttgtct tactttaagt cattggttta aatctaaaga acaagtgcat
     9601 acaaatcaaa aaatttacgt atacaattgg ggcgaatata ttgatccaga gttaattaag
     9661 aaatttgaaa aagaaactgg cattcaagtc gtttatgaaa ctttcgattc aaatgaagcg
     9721 atggaagcca aaattcgcaa tggcggtaca cattatgatg ttgcttttcc tagtgaatat
     9781 acagttcaaa aattgaaaag agatcattta ttgttaccaa tagatcataa taaggtacct
     9841 aatattaaaa atttagattc agattatatg aatatgtcat ttgatagagg caataaatat
     9901 tc
//
LOCUS       DAMQYT010000089         9845 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMQYT010000089 DAMQYT010000000
VERSION     DAMQYT010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9845
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.089"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..253
                     /locus_tag="PGA33_001621"
     CDS             <1..253
                     /locus_tag="PGA33_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473429.1"
                     /translation="EQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSE
                     LNKVEAYYAQIETQIQQTSTEKIATEKTSYLINYMNAVA"
     gene            268..969
                     /locus_tag="PGA33_001622"
     CDS             268..969
                     /locus_tag="PGA33_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDG6473430.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            966..2009
                     /locus_tag="PGA33_001623"
     CDS             966..2009
                     /locus_tag="PGA33_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDG6473431.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            1999..2628
                     /gene="vraR"
                     /locus_tag="PGA33_001624"
     CDS             1999..2628
                     /gene="vraR"
                     /locus_tag="PGA33_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HDG6473432.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMDDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(2744..3961)
                     /locus_tag="PGA33_001625"
     CDS             complement(2744..3961)
                     /locus_tag="PGA33_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDG6473433.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
     gene            complement(4239..4514)
                     /locus_tag="PGA33_001626"
     CDS             complement(4239..4514)
                     /locus_tag="PGA33_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDG6473434.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            complement(4521..4985)
                     /locus_tag="PGA33_001627"
     CDS             complement(4521..4985)
                     /locus_tag="PGA33_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDG6473435.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            5132..5332
                     /locus_tag="PGA33_001628"
     CDS             5132..5332
                     /locus_tag="PGA33_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HDG6473436.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHE"
     gene            5335..6591
                     /locus_tag="PGA33_001629"
     CDS             5335..6591
                     /locus_tag="PGA33_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDG6473437.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAGKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            6681..7211
                     /locus_tag="PGA33_001630"
     CDS             6681..7211
                     /locus_tag="PGA33_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDG6473438.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(7471..8616)
                     /gene="yfkAB"
                     /locus_tag="PGA33_001631"
     CDS             complement(7471..8616)
                     /gene="yfkAB"
                     /locus_tag="PGA33_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HDG6473439.1"
                     /translation="MRNNKPKISIQNDPWEAYNDVKEHGQLTLSNIEFTTTNLCNMRC
                     SHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPLLK
                     YAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQPPL
                     KAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEIHP
                     MYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLSRL
                     RNAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLSSD
                     LAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            complement(8661..9707)
                     /locus_tag="PGA33_001632"
     CDS             complement(8661..9707)
                     /locus_tag="PGA33_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDG6473440.1"
                     /translation="MPVYKDDNTGKWYFSIRYKDVYGNNKRKMKRGFERKKDAKLAES
                     EFIQNVKYGYSDNQPFEYIFFDRLKNENLSARSIEKRTTEYNTHIKERFGNIPIGKIT
                     TTQCTAFRNYLLNDAGLSVDYARSVWAGFKAVINYAKKHYKLLYDPTLSVTPIPRTKP
                     QAKFITREEFDEKVEQITNDTSRQLTRLLFYSGLRIGEALALQWKDYDKIKGEIDVNK
                     KINLSNREIEYNLKKESSKGIIPVPKLIREMLKNMYNESSKRYKYFDENYFIFGGLEP
                     IRYVTYSYHFKSVFPNLKIHHLRHSYASYLINNGVDMYLLMELMRHSNITETIQTYSH
                     LYTDKKHQAMSIFD"
BASE COUNT         3377 a         1484 c         1672 g         3312 t
ORIGIN      
        1 agaacaactt agcaagttaa ttgacagtct aatgctaaca ttagaaaata aatatattga
       61 tattgctgaa gcatttcaaa ttcaatgtgc aagagaaatc aataatcaag aaattgaaaa
      121 tattaaatca gagttgaata aagttgaagc atattatgca caaattgaaa ctcaaattca
      181 acaaacttca actgaaaaaa tagcaacaga aaaaacatcg tatctaataa attatatgaa
      241 cgctgtggca tagaaaggcg gcgaaacatg acacacaaat atatatcaac gcaaatgttg
      301 atcattttta ctgcattaat gattattgcc aatttttact acatattttt tgaaaaaatt
      361 ggctttttac tcgttctatt attgggatgt gtattagttt atgtaggata tctttatttt
      421 cataaaatac gtggcctttt ggcgttttgg ataggcgcgc tattaattgc attcacatta
      481 ttgtctaata agtatacaat catcatcttg ttcgtctttt tattattact tattgtgcgt
      541 tatttaatac acaagtttaa accaaaaaaa gtagttgcga cggatgaggt tatgacttca
      601 ccatctttta ttaaacaaaa gtggtttggt gagcaacgta caccagttta tgtatataag
      661 tgggaagatg tacaaattca acatggaatt ggcgacctac atattgactt aacaaaagct
      721 gcaaatatta aggaaaataa taccattgtt gttagacaca ttttaggtaa agtgcaggtt
      781 atattgccgg ttaattacaa tattaattta catgtagctg ctttttatgg aagtacttac
      841 gtgaatgaaa aatcatataa agttgaaaat aacaatattc atattgaaga aatgatgaaa
      901 ccggataact atacagttaa tatctacgta tcaacgttta tcggagacgt agaggtgatt
      961 tatcgatgaa ccactacatt agaacaattg gttcaatgct catcttagta tatagcatgc
     1021 tagctgcatt tctgttcatc gataaagttt ttgtaaatat catctatttt caaggtatgt
     1081 tttatacaca aatattcgga ataccagttt ttttattttt aaatctcatc atcatattat
     1141 tgtgtattat tgttggttcg gtactcgctt acaaaatcaa tcagcaaaat gattggatta
     1201 agacgcaaat tgagcgttca atggaaggcg aaacagttgg cattaatgat caaaatatag
     1261 aaatatatag tgaaacgtta gatttatacc atacactcgt acctttaaat caagaattgc
     1321 ataagttgcg acttaaaact caaaacttaa ccaatgaaaa ttataatatt aatgatgtga
     1381 aagttaaaaa gattattgaa gatgaacgtc aaagactagc acgagaactt cacgattctg
     1441 ttagtcagca actttttgcg gcaagtatga tgctatctgc tatcaaagaa acgaagttag
     1501 aaccaccatt agaccaacaa attcctattt tagagaaaat ggttcaagat tcgcagttag
     1561 aaatgcgtgc tttgctgtta catttaagac cgcttggttt aaaagacaaa tctttaggtg
     1621 agggtattaa agatttagtt attgatttac aaaaaaaagt gccaatgaaa gttgtgcatg
     1681 aaatacaaga ttttaaagtg cctaaaggta ttgaagatca tttgttcaga attacacagg
     1741 aagcaatttc gaatacattg cgtcattcaa acggtacaaa agtgacagta gaattgttta
     1801 ataaagacga ttatttattg ttgagaattc aagataatgg taaaggtttt aatgttgatg
     1861 aaaaattaga acaaagttat ggacttaaaa atatgcgtga aagagctttg gaaattggtg
     1921 caacgttcca tattgtatca ttgccagatt caggtacacg tatcgaggtg aaagcacctt
     1981 taaataagga ggattcgtat gacgattaaa gtattgtttg tggatgatca tgaaatggta
     2041 cgtataggaa tttcaagtta tctatcaacg caaagtgata ttgaagtagt tggtgaaggc
     2101 gcttctggta aagaagcaat tgccaaagcc catgagttga agccagattt aattttaatg
     2161 gatttactta tggatgacat ggatggtgta gaagcgacga ctcagattaa aaaagattta
     2221 ccgcaaatta aagtattaat gttaactagt tttattgaag ataaagaggt atatcgtgca
     2281 ttagatgcag gtgtcgatag ttacatttta aaaacaacaa gtgcaaaaga tatcgccgat
     2341 gcagttcgta aaacttctag aggagaatct gtttttgaac cggaagtttt agtgaaaatg
     2401 cgtaaccgta tgaaaaagcg cgcagagtta tatgaaatgc ttacagaacg agaaatggaa
     2461 atattattat tgattgcgaa aggttactca aatcaagaaa ttgctagtgc atcgcatatt
     2521 actattaaaa cggttaagac acatgtgagt aacattttaa gtaagttaga agtgcaagat
     2581 agaacacaag ctgttatcta tgcattccaa cataatttaa ttcaatagtt catatcgaat
     2641 taagaaaagt tacttacgcc aatcacaata taacatcaaa tagacaccaa aaaacagagg
     2701 ttttcgcaat aatcattagc gaaaacctct gttttgttgg tgtttagtct tttttaagct
     2761 tatcaacgat tttgtctttt atatttttat catcttctgg ataggtatca ctaggatgtt
     2821 catcaacatg atacgtacgt tgatgtgttg cattactatc ttcatcatat tcgtatctag
     2881 tgttctcatt ataatgattt ttattattgt catcatgttc taatgctgct tcttcaggtg
     2941 ttttaccttt aattacacta cgctgatgaa tgattgcatt gatttcagca ccgacaatta
     3001 taataaaact tgtgatatat aaccatagta acaaaatgat gatacccgcg atactgccat
     3061 atgttttaga atagttacca aaatttgaaa tataccaacc aaaaccaaat gaaccagcta
     3121 accaaataat tgaagtaaat actgcacctg gtaatactga cttaagcttc gttttaacgt
     3181 taggtgcaac cgaatataac acgataaata tgataaatat aataatgatt ggtaacacaa
     3241 ttctaataag gttaaaaatc catttcactt gttcgtcaaa tccaagtgga ccgaatagga
     3301 aatgactaat tacagaacca agcgttggta atgctagagc aactacaaac acaacgccca
     3361 taactacagt gaagacaaca cttagtagtt ttaatacgat tccattacgg ctatcttcta
     3421 catcgtaagc aacattgaaa gaattcataa ttgcagtcat tccatttgaa gctgaccaaa
     3481 ttgctaaaat caaaccgata gataataagc caccactgga gttttgagtt atatcaccaa
     3541 ttacactctt aattagagta gatgtttcag cgggtgcatt gcttaacata ttagtaattt
     3601 gactctgctt aatattgaaa aatggtaata atgttaatag gaaaagcaac atagggaaca
     3661 tagcaagtac gaaatggtaa gtcatttgtg ctgctagtcc tgaggcatca tcttttccta
     3721 tgcgatagat gagatatgat aagaaattag agtctttctt atattttgca ggtttattta
     3781 atcttgataa gaagaaaacc tgatcatctt ttttaggttt cttagattgg aattgttgag
     3841 gttcaatata tgtacgatca acatcaattt tgggattact tttatttttc ttttttgact
     3901 cctcttgcgc ttccttaaca gaattaaggt attttgaaga agagtgatcc tttttcgaca
     3961 tgtgcactcc ttaacctttc tctatgtaat gttttataaa gtaactatta gtatttatcc
     4021 aattataaaa aaactaaaca atgatatgct ttaaaaatga taatattata gaaattaatt
     4081 gatttaaatc tttcataggc tattttgatt taaaattaaa acaaaggcaa tactgaaaca
     4141 atgcagtttg gccaatcatt gtattatcat ttaataatga taactatgaa aggcgtacac
     4201 caaattgtta agtattgcct tataaaaaca aaatcatctt agcgttgatt ttttctatta
     4261 acaaatgttt ctttcgcatc ctttaatgaa cgttctaatt caggattatt acgacgaatt
     4321 tcttcaacaa catctttcca gtataaaact tcgtctttaa tcttgctgac tttagaaggc
     4381 ttacgagtgc ggttaccatt ttttgcatct ttaactgatt gaactaaagc ttgacgtgta
     4441 gatttatcag ctaaactaat tgcaccacca attacggcac caattaaaat accaggaaca
     4501 aatttatttt ccataaaaaa ctacccctct ttcaaatttg catcttttac gatgtagtct
     4561 attaaattat cacaagatga taataccatg tcgtatacac cttcaaaatt attcgtgtag
     4621 tatggatctg gtacatcact ctcttccata ttactaaatt ctaacagttt gaacaattgt
     4681 cccttaagat taggattgat agatttaata ttatcaacgt tactttgatc catagccaca
     4741 atgtaatcaa aatcatctgt cgcttcgaat aattcactaa tcatgccatc aaatggaata
     4801 ttgtgtttgt tgagaatttt ttgtgtacct tcatgaggtg gctctcctaa attccagcta
     4861 ccagtacctc ttgaatgtac tttaatatca tgaatatttc tgtctttaag tctttgtcgc
     4921 atgattgctt ctgccattgg agaacgacat atattgccaa gacagacaaa tgctacatct
     4981 accattttga ttcctccaaa ctatgtagtt atatccccat tttatagcga ctttaaacaa
     5041 taagaaagca gattatataa aattctatta aagtttatta aattgtgata ctttgataac
     5101 ataactatta ttagaggtga acattgtggc tatgacaaat gaagagaaag tattagctat
     5161 tagagagaag ttaaatattg ttaatcaagg attattagat cctgaaaaat ataaaaatgc
     5221 aaatgaagaa gaattaacag atatatatga ttttgttcaa tcaagagaaa gattgtcgcc
     5281 aagtgaagtg acagctattg ctgacgcttt aggacaattg cgacacgaat aggagtggga
     5341 attttgacta attacaaaga aaagttacaa caatacgctg aactattagt taaggttggt
     5401 atgaatgttc aaccaaaaca gccagtattt attagatcat cagtagaaac attagaatta
     5461 acgcatttaa ttgtagaaga agcatatcat tgtggcgctt cagatgtacg tgttgtatat
     5521 agcgatccaa ccttaaaacg cttaaaattt gaaaatgaat ctgtggaaca ttttgctaat
     5581 catgagataa aatcatatga tgttgaagcg agaatggatt acgttaaacg tggcgctgct
     5641 aatttagcac ttattagtga agatcctgat ttaatggatg gtatcgatag ccaaaaatta
     5701 caagcatttc aacaacaaaa cgcacgcgca tttaaaggat atatggaaag tgtacaaaaa
     5761 aatcaatttc catgggtcgt agctgctttt ccatctaaag cttgggcgaa gagagtgtat
     5821 cctgaactat ctgtagaaga agcatatatt aagtttattg acgaggtatt cgatattgtg
     5881 cgaattgatg gtaacgatcc agttgaaaat tggcgtcaac acatcgctaa tttaagtgtt
     5941 tacgctcaaa agttacaaca aaaaaattac catgcgttgc attatgtatc tgaaggtact
     6001 gatttaacag taggtttagc gaaaaatcat atctgggaag atgctacaag ttatgttaac
     6061 ggcaaggaac aagcttttat tgctaatata ccaactgaag aagtatttac agcaccagat
     6121 cgaaatagag tagatggata tgttactaat aaattgcctt taagttataa tggcacaatt
     6181 atcgatcaat ttaaattgat gtttaaagat ggagagatta ttgatttttc agctggaaaa
     6241 ggtgaagcgg tattgaaaga tttaatcaat actgatgaag gttcaagaag attaggtgaa
     6301 gtagcattag tacctgatga ttcaccgata tcaaatcgta ataccatttt ttacaatact
     6361 ttatttgatg aaaatgcagc atgtcattta gcgattggat ctgcttacgc ctttaatatt
     6421 caaggtggaa cggaaatgac tgttgaggaa aagattgcaa gcggattaaa tgattcaaat
     6481 gtacatgtcg atttcatgat tggtagtagt gatttgacta tttatggcat attcgaagat
     6541 ggttcaaaag aactagtatt tgaaaatgga aattgggcat caacatttta ataaatgtta
     6601 ttttgaggtg ctgagtagga aatgaaacat gtatttaaag gtcaaatacg tgattaaagt
     6661 atagattggg agataaaata atgacaaatc aggacagacc aatgaaatct atgtcagaat
     6721 caaaatgtta taaaaataga caagttttcc ctcaagatac gaatcaccat catacaatgt
     6781 ttggtggtac attgatggct aatattgatg aaattgcagc aatcacagct atgaaacatg
     6841 ctggtgcgca agtagttacc gcatctacag actcagtaga tttcttaaag ccgattaaaa
     6901 caggggacat attacaatac gtagcgatgg tttcatacgc tgggactagt tcaatggaag
     6961 tggtcgttca aattagaatt gacgatgtat ttaataacaa gcatgattta gctgcattaa
     7021 gttacttaac atttgttgcg ttagatgatg aaggcaaacc taaacatgta cctggtgtat
     7081 atccagaaga tgatgttgaa aaatggtttt atgatacagc accacaacgt gttgaacgaa
     7141 gaaaagcacg tcgcattgaa agtaaacaaa caattgaata tttagctcaa gtgcaacata
     7201 ttagagacta aaaatataaa gacagattgt taaggatgaa ttgaactgtt atgttcgaat
     7261 attccaccta caatctgtct ttttgattat gcacgtttat gcttttagtt aaaatttacc
     7321 ccttttatta tagactatta gtcctattaa atgcccttta ttatttaagt agagtactgc
     7381 cagcaattaa ttgtttttta ttttataata gcattaaaat atgcggagac tacttatcga
     7441 taaagtgctt ctccgcataa ttaagagttt ttaaaattgt ataatttgtg gttgtttgtg
     7501 catatgacgc tcattatctt taaaatccat attcggatag tacatatttt taacaagaac
     7561 atttggtcct aaacaactaa actcggaaca atgacaattt aatgatttag caagatcaga
     7621 ggataaccat ttatcaaata catctgttaa tttatctttt tgtatattcg aaattgtacc
     7681 tgtttcatct ccgaaatcag ttacgattac attacctgta aatacattga catttaaacg
     7741 actacggcca tccgggtcat ttctagtcgt tacattttta gcatttctta aacgtgatag
     7801 taacttttga tcttcatcat cctttaagca tggaaacaca ggcaaagtac caaataacat
     7861 ccaaatatct tcatctctga aatccaatat atcatgaatt gtctttttca tttccgctag
     7921 agttaacaca tttaattgac ttgcaaagtc agctggatac atagggtgaa tctcgtgtct
     7981 gctacatttc atatcatgaa cgacttcttg atgtattttt cgtaaatgtg gtagcgtact
     8041 ttgattgagc attgtttccg cagatacaaa cattccttgt tctgataatg tacgtgcatt
     8101 cgaaatcatt tgttcatata attttaactt agcttttaac ggtggttgct tcttcattgc
     8161 gccaaagcca acatttgcga attcatcagt tgttccccag ttgtgtgaga tatgcataac
     8221 atcgatatat tcagcaatat ctaaataacg atcttgaggc aatgttaggt ttgaattcat
     8281 ttgtgtatat atacctcgat gatgtgcata ctttaataga ggtttaacaa catttctaat
     8341 agacttttta gaaaacattg gttcgccacc tgtaatagac atcgttcgca gatttggaat
     8401 ttcatcaagt ctacgataaa ttaagtccat atctaaaggc tcggggtcga cagtttgtaa
     8461 agtataacca actgcacagt ggctacaacg catattacaa agatttgtag ttgtaaattc
     8521 gatgttactt aaagttaatt ggccatgttc tttaacatcg ttatatgctt cccatgggtc
     8581 gttttgaata cttattttag gcttgttatt acgcatttta taaactcctt aattgttatt
     8641 tgataccaat ttgataccgt ttaatcaaat atgctcatag cttgatgttt tttatcagta
     8701 tataaatgag agtacgtttg aattgtttct gtaatgttag agtgcctcat taattccatt
     8761 aataaataca tatctacacc attattaatt aaatagcttg cgtacgagtg tcttaaatgg
     8821 tgtattttta gattcgggaa tacagattta aaatgatacg aataggtaac gtatctaata
     8881 ggttctaacc ccccgaatat aaaatagttt tcgtcaaaat atttatatct tttagaagat
     8941 tcattataca tgtttttaag catctctcta attaattttg gtacaggtat tatcccttta
     9001 gaactttctt tttttagatt atattcaatt tctctattac ttaaattgat tttcttattt
     9061 acgtcaattt cgccttttat tttatcgtaa tctttccact gcaaagctaa agcttctcct
     9121 attctaagac cagaataaaa taacagtcta gttagctgac gagaagtatc atttgtgatt
     9181 tgttctactt tttcatcaaa ttcttcacga gtgataaatt tagcttgtgg ttttgttctg
     9241 ggaataggag ttaccgataa tgtggggtcg tataagagct tgtaatgctt tttggcgtaa
     9301 ttgataactg ctttaaaacc tgcccacaca gatcgtgcat agtcaacaga aagacctgca
     9361 tcgtttaaca aataattcct gaaagcagta cattgcgtag tagtgatttt gccaataggg
     9421 atatttccga acctttcttt tatgtgagta ttatattctg tagttcgctt ttctattgag
     9481 cgtgcagaaa gattttcatt ttttaaacga tcaaaaaata tatattcaaa gggttgattg
     9541 tccgagtatc catatttaac attttgtata aattcgcttt cagctagttt ggcatctttc
     9601 ttacgttcaa acccacgctt catttttcgt ttgttattac cgtatacatc tttatatcta
     9661 atggaaaaat accatttacc tgtattatca tccttatata ctggcatttt gcttttccct
     9721 cctcaaaatt ggcaaaaaat aataagggta ggcgggctac ccgtgatttt agtactaggt
     9781 actaaatgtg atataataaa ataaaaagta ggtgatgaaa tgtgtgtaaa atttactgac
     9841 gcaga
//
LOCUS       DAMQYT010000090         9626 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMQYT010000090 DAMQYT010000000
VERSION     DAMQYT010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.090"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..180)
                     /locus_tag="PGA33_001633"
     CDS             complement(<1..180)
                     /locus_tag="PGA33_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="sigma factors are initiation factors that promote
                     the attachment of RNA polymerase to specific initiation
                     sites and are then released; primary sigma factor of
                     bacterium; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDG6473441.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFD"
     gene            complement(404..2203)
                     /locus_tag="PGA33_001634"
     CDS             complement(404..2203)
                     /locus_tag="PGA33_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDG6473442.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQNLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPE
                     PIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDF
                     YAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEKGQETIE
                     SLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            complement(2264..3082)
                     /locus_tag="PGA33_001635"
     CDS             complement(2264..3082)
                     /locus_tag="PGA33_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDG6473443.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDINPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(3093..3716)
                     /locus_tag="PGA33_001636"
     CDS             complement(3093..3716)
                     /locus_tag="PGA33_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDG6473444.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHKVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            4051..5442
                     /locus_tag="PGA33_001637"
     CDS             4051..5442
                     /locus_tag="PGA33_001637"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HDG6473445.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(5592..6338)
                     /gene="recO"
                     /locus_tag="PGA33_001638"
     CDS             complement(5592..6338)
                     /gene="recO"
                     /locus_tag="PGA33_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDG6473446.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYAALAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(6366..7265)
                     /gene="era"
                     /locus_tag="PGA33_001639"
     CDS             complement(6366..7265)
                     /gene="era"
                     /locus_tag="PGA33_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDG6473447.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(7266..7670)
                     /gene="cdd"
                     /locus_tag="PGA33_001640"
     CDS             complement(7266..7670)
                     /gene="cdd"
                     /locus_tag="PGA33_001640"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDG6473448.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(7681..8025)
                     /locus_tag="PGA33_001641"
     CDS             complement(7681..8025)
                     /locus_tag="PGA33_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDG6473449.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(8028..8495)
                     /gene="ybeY"
                     /locus_tag="PGA33_001642"
     CDS             complement(8028..8495)
                     /gene="ybeY"
                     /locus_tag="PGA33_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDG6473450.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            complement(8496..9443)
                     /locus_tag="PGA33_001643"
     CDS             complement(8496..9443)
                     /locus_tag="PGA33_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDG6473451.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
BASE COUNT         3028 a         1781 c         1320 g         3497 t
ORIGIN      
        1 atcaaagaaa tcatccattt gatcagagtc gatatcaaaa ttctgaagtt tttcagcaat
       61 ttcttcatga cttaaatgac cctctttttt acctttttca attaattgct tcttaacatc
      121 ttctaatgtt aatgtcggat caattgtttg ttttttaatt ttaactgtgt tatcagacat
      181 gaaacggcct cccgatttta aatatgaaca ttcgaaattt attcaatatt gctattttaa
      241 actaaattct taattaattc atccatattt ttaattttat tttacaaatt ggaactaatc
      301 ccaatattta tttttcaata gtgttgttta aatgcggtaa ctatcattta cccttataca
      361 ttttgacata atcattattc gtattattct ttaaaatcac atgctacatg cgttctttat
      421 tcttagcaac aatttgctgt aaatagtatt tttgtaattc tacatcgcca atccttgtag
      481 cttcccttaa tttatgattc aatgactcaa ttgtttcttg tcctttttca ttaataacat
      541 tgacataatc atcaatttca ttttcatatg gttcgtcatt caaattatat tgttctaagc
      601 taattagtgt ttctctcaac tcatttgaat taacatactg cacagcatca ctgatattat
      661 attgatcatt ttccgcataa aaatcatgta agacttcgaa tacatattta aaatgctgat
      721 ttgtgaagtt atccttatca acactttcat aataatttaa aaatgtatct ttatctctca
      781 ttaaatgttt taaaaatgct cgctccgctt tttcttgacg gctcaaattg tcaaattgtg
      841 ccataccaat tggctcaggt tcaatgtaac cgccatactc atcttctgga taataattgg
      901 ctggtgcttg attgaattgt atttcgttag ctaattgctc aggactaaca ttgaaaaatg
      961 gtgcaacatc atttaaagcc ttttgttgca aaatcgatga tttcataagc gaaatatcat
     1021 gacttagttc tttcaaataa cgttcatatg aaaggtcatt atgtgcaatt tcatctttta
     1081 atatactcac tttataatgt gcaaatgact ttttgtcatt ttttacaaaa gcagtaaatg
     1141 catcgttgcc atacttacca atgtattcat ccggatccat gcctgatggc aattgtataa
     1201 caaatacatt tagcccttgc tgtaacaaat tttgacctgt tttaagtgtt gcttcactac
     1261 ccgcaaaatc cccatcaaac attaatgtta tatttgatgt taactttcgt ataaaagtaa
     1321 tatgttcatc tgacaactgt gtacccattg ttgcaacaac gtttttcaag ccagcagtat
     1381 cagattttat aacatccata aaaccttcta gtaatacgat ttcatctaat tttctaattg
     1441 atttacgcgc tttatctaag ttgtataaca actttctttt ttgaaagata ggtgtttcag
     1501 gactatttaa gtattttggt tcttgaccgg tatatgttcg acctgaatat ccaacaattc
     1561 ttccttgcgc atttttcaaa ggaaacataa tacgatttcg aaatctatcg taataactga
     1621 aattttcttc gttacgtgat aataatccgg cttcatatgc taattcaata tcgtaaccct
     1681 ttttttgaag aaaatcatga caaaaatgtg agctatcggg tgcaaagcca atgcctcgct
     1741 ctttaataag cgcatctgta aaaccacgtt cttgtaagta cgttaatgct tgttcgcctt
     1801 cgactgtctt tgttaaagcg taataataaa attcttgtat taactcatgc atttcaatca
     1861 tttgtaaatc atcagaagca atttgaacat ttgagttaga ttgtgttgcc tcaatatcta
     1921 cagcaacatt aactctatca cctaattctt taaccgcttc aacaaatgat atgtctttaa
     1981 tttcttgagt aaattgaaaa acattgccac cttttttaca accaaaacaa tgacaaattt
     2041 gtttatcttc agaaactgta aatgaaggtg tcttttcatc atgaaaagga cacaaaccta
     2101 tataattgcg tcctctcttt tctaatttta catattcact taccaagtct aaaatgtcgg
     2161 ttttatcttt tatttcatta atgatcgatt gatctattcg caaattaaat cacctattaa
     2221 gcattagtta tactattata cctaattttc gacaaaaatg aaatcatttc gatttatttt
     2281 gttcaatata atggattata tcgtttgctg tttcttcgat tgctttttga gaaacatcaa
     2341 tgacaggaca tccaatttca cttacgattt cttcaaagta attcaattct tcttgaattc
     2401 gtgcttctgt tgcatatcga gctgtgtcac ctagtcctaa ttgttttagt cgctcttttc
     2461 taatgcgatt taatttttct tcacttattt taagtgcgat acatttcttt ggattaatat
     2521 catataagcc atctggcggt gtcacttctg gtacaatcgg tacattcata actttgtaac
     2581 tcttatgcgc taaatactga gataatggtg tctttgaagt tctcgaaata ccaagtaaaa
     2641 caatatcagc tttaggtaat cctttaggat ctttaccatc atcatattta actgcaaact
     2701 ctatcgcatc aattttcttg aaatatgcat catctaatct atgaacgata cctggctcat
     2761 tataaggttt ttcttcaacc gaagcagata ataaatccat taatggcccc atgatatcga
     2821 cagacttcaa ttggaattct gctactttct cactcatata ttgcttcatt tcaggtttaa
     2881 taagtgtata aacaatgata gcatttgtat cttttgcaac ttgaatcact tcatcaacat
     2941 cttcaaaaga ttcaatatat ggatatctta ataattcatt tttacattgc ttaggattga
     3001 attgtgaaat acctgcccta gcaactaact ctgccgtttc acctatagaa tctgaagcta
     3061 cgataatttt aattttttcc attacttctc acctattctt tatataatgc tactaataac
     3121 ttagctattg ttgttttgga aattcttcca attacttcat acttttgatt atcttttttt
     3181 cttacaattg gaatcgaatc aatttctttt tcaatcattc tatctgctgc gtatatgact
     3241 aattcgcttt cctctaaata agtgacatta ggcatacgtg tcatatttac actgataggt
     3301 actttatgaa tatctgctcc aatcattgaa gctcttaata aatcttttct tgaacacaca
     3361 ccaacaaaat cgttatcttc attaataata aataatgtac ttacatcttc taaaaaaatt
     3421 gtacaaatag cgtcataaac tgttgtattc tctcttagca caacaggttg agacatatag
     3481 tccttaacct caaattgtcg aagtttttca ttaaaaaatt tgccttttga tttacctgaa
     3541 taataatatc caactcgggg acgcgcttct aaaaaacctg acattgttaa tatcgctaaa
     3601 tctggtctaa gcgttgctct tgttaaattc aacttatctg ctatttgttc accagtaatg
     3661 ggtcctttag ttttaacaat ttcgatgatt cgttcttgtc tttgactgag ttctataggt
     3721 cttcacccct tttttattca cctattatta taccgatatt tctttcatgt cacaaacaca
     3781 ttaattttac ttgcctttaa ataatctatc aattagaata aacgtacagc gagttaagga
     3841 tagtgtaagc ttaacaataa gattggcgca acgaatcatt ttaaaataaa agcgagtgac
     3901 tacactaatt tgggtggaac cgcgggttaa atacgtaact tattttgtat tatatacaat
     3961 ttaaaatatt aactcgtccc atggaagtaa agcatattta ctatgctgtt attttccatg
     4021 gggcgttttc atgtatgagg agaggtaatt atggcaaaag atatggatac aattgtttca
     4081 ttagcaaaac acagaggttt tgtgttccct ggtagtgata tttacggtgg tttatcaaac
     4141 acatgggatt atggtccttt aggtgttgaa ttaaagaata atgttaaaaa agcttggtgg
     4201 caaaaattca ttacacaatc accgtttaac gttggtatcg atgctgcaat cttaatgaat
     4261 ccaaaagtat gggaagcttc aggacactta aacaacttca acgacccaat gattgataat
     4321 aaagatagta aaattcgata tcgcgctgat aaattaattg aagattatat gcaagatgtt
     4381 aaaggtgatg aaaacttcat tgccgatggt ttaagttttg aacaaatgaa aaaaattatt
     4441 gacgatgaag gtattgtttg tcctgtaagt aaaactgcta actggactga aattcgccaa
     4501 ttcaatttaa tgtttaaaac attccaaggt gtaactgaag attctacaaa tgaaattttc
     4561 ttacgtcctg aaacagcaca aggtattttt gtaaactata aaaacgtgca acgttcaatg
     4621 cgtaaaaaat taccatttgg tatcggtcaa attggtaaat cattccgtaa tgaaatcact
     4681 ccaggtaact tcattttcag aacaagagaa tttgaacaaa tggaacttga attcttctgt
     4741 aaacctggag aagaaatcga atggcaaaat tattggaaaa cttttgcaag tgactggtta
     4801 acaagcttaa atatgagcag tgaaaatatg cgtttacgtg atcatgatga agatgaatta
     4861 tctcattact caaatgcaac aactgatatt gaatataaat tcccatttgg ttggggtgag
     4921 ttatggggta tcgcaagtcg tacagacttc gacttacgta aacatgctga acactctggt
     4981 gaagatttca gataccatga tccagaaacg aacgaaaaat atattccata ttgtatcgag
     5041 ccatcacttg gtgcagatcg tgtaacatta gctttcttat gtgatgcata tgatgaagaa
     5101 ggcgttgaag gtagtaaaga tgcacgtaca gttttacact tccatcctgc attagcacca
     5161 tataaagcag cgattttacc tttaagtaag aaattatctg gcgaagcgat taagattttt
     5221 gagcaattaa gttctaaatt ctcaatcgat ttcgatgaat cacaatctat cggtaaaaga
     5281 taccgtcgtc aagatgaaat cggtacacct tattgtgtaa cattcgactt tgattcatta
     5341 gaagataatc aagttacagt acgtgacaga gattcaatgg aacaagttcg tatgccaatc
     5401 tcagagttag aagctttctt aactgaaaaa acaaaattct aattaaaaag actttccacc
     5461 atatgtggtg gctatataat ttataaaaag agatactggt tagtaaaatt gccagtatct
     5521 cttttctatt caaaaaggta ttcaaataaa cacatctatg tggctacttg aatacctttt
     5581 attattttat gttattgttc caatcttttt aattggttga ttagtttctg acttttaaaa
     5641 aacatacctg catattcacg atataacatt aaaatgatat ctgacatttc atcaataatt
     5701 tcttgatgga tattcaatga attcatttta tctatcggta atttttgtaa tacatctaat
     5761 aaatatagtg ttttattcga taatataact gcatgtacat ctttagaagc ctcttgcctt
     5821 gaaatcgcac cgtcaaactt aaaactataa cctattaaat ctgcttgtgt gtcattacca
     5881 ctcacagcac agcgattaaa tgatgcagta aaaccaaatc gtttcatgca ctttaacata
     5941 actacgactg acattaactg tgcagatgta cctgattcta ttttttcaag aacaaattgt
     6001 aataattgat agttatatgg tgcaatgtca ccttcatcca ttgagcgctc aatagtttca
     6061 gctgccagag cggcataact gcttacgtaa aggtccattt gtaatttata atgttgacta
     6121 ataacatcta cagaatttaa cgttcccata cctcgccact gattgtaaat aaacaaacca
     6181 taaacaaaca attgcgtttg cgcttgtaaa cccgtcttaa cttttttagc acgccttgcc
     6241 ataagtggta cttttgcacc atgctcattt aaaatcgtga taattttatc agattcacca
     6301 taatcaactg ctttgatgat aatccctttt tggcgcatta acaattatct tcaccacttt
     6361 taagattaat cttggtcttc aacataacca atttggcgaa taaagttaac tttgtttcgc
     6421 cagtctcttt gaactttgac ccataattct aagtaaactt tagagcctag aagcatttct
     6481 atatcacgtc tcgcacgttt tcctacttct tttaactttt taccgccttt tccaatgaca
     6541 attccttttt gcgaatctct ttcaacatat atagttgctt cgatatgaac acgatcttcg
     6601 ctttctttaa ccatacggtc cacattaaca ccaatcgcat gagggatttc ttcacttgta
     6661 agatgaagga ttttttcacg aatgatttca cccactacaa attgttcagg atggtctgaa
     6721 atttgatcat ctggataata tttaggtcct tcgggtaaat acgtctttaa aacatcaata
     6781 aaatgatcga catttagccc ttctaatgct gaaataggta caatctctgt aaagtccata
     6841 taactttgat attcttcaat ctttggcatt aattcatctg gatgcactaa atctatttta
     6901 tttaatacta aaaatactgg tgtcttaaca tttttcaaca tttctataat atattcatcg
     6961 cctcgtccaa tttcctcatt ggcattaacc ataaacatga ttgcatctat ctcagataat
     7021 gtatttttag cgactttcat catatagtca cctaatttgt gtttaggttt atgaatacct
     7081 ggcgtatcaa tgaatataat ttgcgcgtca tctcttgtca taacaccttg aattttattt
     7141 ctagttgttt gagctttatc ggacatgatt gctattttat ggccgatcac tctattaaca
     7201 aatgttgact ttcctacatt tggtctacct ataattgaaa caaatcctga tttatgttct
     7261 gtcatttatt ctaaatcctt tcctgaaaat ccaaatggta gtaactctgc gactgtcatc
     7321 ataaccatat ctcctttatg atttgtcata tacacaggca tatcatcatc acataattcc
     7381 ttcaaaactt gacgacatgc accacaaggt gatgacggtt tatctgcatc tacggttaca
     7441 gttattgatt caaaatcacc tggtctgtat ccttgagaaa ttgccgatac caaactagct
     7501 cgttcagcac atatcgataa tggataagaa gcattttcta cattggtacc ataaaaagtt
     7561 ctaccgtctt tcgtttttaa ataagcccct actttaaatt gactgtatgg cgaatatgat
     7621 tcttgttgtg cttttctaac ttcttgaaaa taatgaggtt gataactcat atatgcctcc
     7681 ctaaaataac gctataaaat gtggtaaaaa tactattaaa cctataataa atgctaatat
     7741 tgaaactata agtacactaa aagccgcaat atctttagcg tatttagcta aatcatgata
     7801 ttcaacggtc actaaatcga caacatattc aatagcagtg tttaaagctt caacagtgag
     7861 aactaatgca atagcaatga gtataaatat ccactcaatc cgattaatat ttagtacgag
     7921 accaaagaca atagcaacaa tcattgcaaa cacatgtaaa agaaatttat agtctttttg
     7981 aattaagatt ttcagcccat caagtgcata tttaaacctt ttcataatta gtctcgtgtt
     8041 aatccatatg cgtttaatat tgtatcttgt cgaccaaaca tttccttttc atccgcttca
     8101 gtcatatgat cataacctaa tagatgcaaa aatccatgta atgctaaaaa tcctaattct
     8161 cgttcaaaag aatgtccgta attgtttgct tgttcttgcg ctacatccgt acagataatt
     8221 atatccccta aaacacgtgg tatatcaaga ccactaaaat caatctctgg ctcatcttct
     8281 tctaaagcaa atgagattac atctgtaact ttatctttat ctctatatgt tcgattaatt
     8341 tcttgtattt cttgtttatc tacaaatgta acagaaagct cagcatcgtc ttctatatgc
     8401 tcttcttttt tagcaaattc taataaatct tcaatttgtt tataccaagc atctttaact
     8461 aagcctgtgt gatcgctaaa atctatcgta aacatttaat tctctccttc ataatgttca
     8521 atgatcttac ttaccaatgg atgtctgact acatcgctct gatctaattt caatatactt
     8581 atacctttaa cgttgtgtaa cctactgacc gcttccttaa gtccactttt aacaccttta
     8641 ggtaaatcga tttgagtttg gtcaccagta actaccattt ttgagccaaa acctagtctt
     8701 gttaaaaaca ttttcatttg cgcatgtgtc gtattctgcg cctcatcaag aattacaaat
     8761 gcatcttcta atgttcgtcc gcgcatatat gcaagtggcg ctatttcgat aatgcctctt
     8821 tcaataaatc gctctgtttg ttcacgccca agaacagtat atagaccatc atataaaggt
     8881 cttaaatatg gatctacctt ttctttcaaa tctcctggta aaaatccaag tgactctcct
     8941 gcttcaacag caggtcttgt taatacaata cgtttaacag cacctttacg gagttgcttt
     9001 gctgcataaa ctacagctaa gaatgtctta cctgtaccag caggacctat accaaatact
     9061 aaatcattat ttttcatggc attaacatat atacgttgcc ccatcgtttt cgcacgaatc
     9121 gtcttaccaa atgcatcttt agttatctct tcatcatata aatctaacag atgttgaatt
     9181 gtgttattat gcgccatttt aatagctgct tcaacatctt taattgtaat attattacct
     9241 aaatcaataa ccttcagcaa attgattaat actgattccg ctttttctac gttttctatt
     9301 tttgtacctt taacggcaac ttcttgtcct cttgcatgga tgacaacatc gaaactctct
     9361 tcaattgctt ttaaatgttc atcattattt ccaattaaag cttgagattg gttcatatcg
     9421 tctatttgta taattccagg catacacgcg ctccttttca tatatcattt atattcattc
     9481 tatgatgctt taatatctac ttaattatat catgcaattg attgatttaa acatctttgc
     9541 attgacttac tcatttttct ttaatttaaa ataatcattt atacttttca ccttgtcata
     9601 aaagcagatt caattttaga ctaatc
//
LOCUS       DAMQYT010000091         9495 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMQYT010000091 DAMQYT010000000
VERSION     DAMQYT010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9495
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.091"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..604
                     /locus_tag="PGA33_001644"
     CDS             <1..604
                     /locus_tag="PGA33_001644"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDG6473452.1"
                     /translation="HVKFYTGILKDREVVITQSGIGKVNAAISTTLLINKFKPDVIIN
                     TGSAGALDESLNVGDVLISDDVKYHDADATAFGYEYGQIPQMPVAFQSSKPLIEKVSQ
                     VVQQQQLTAKVGLIVSGDSFIGSVEQRQKIKKAFPNAMAVEMEATAIAQTCYQFNVPF
                     VVVRAVSDLANGEAEMSFEAFLEKAAVSSSQTVEALVSQL"
     gene            624..1151
                     /locus_tag="PGA33_001645"
     CDS             624..1151
                     /locus_tag="PGA33_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDG6473453.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            1152..2252
                     /gene="yqeH"
                     /locus_tag="PGA33_001646"
     CDS             1152..2252
                     /gene="yqeH"
                     /locus_tag="PGA33_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDG6473454.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            2266..3072
                     /gene="aroE"
                     /locus_tag="PGA33_001647"
     CDS             2266..3072
                     /gene="aroE"
                     /locus_tag="PGA33_001647"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDG6473455.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELEGFNITIPHKERIIPYLDYVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            3076..3366
                     /gene="yhbY"
                     /locus_tag="PGA33_001648"
     CDS             3076..3366
                     /gene="yhbY"
                     /locus_tag="PGA33_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDG6473456.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATHSELVQVIGSMIVIYRESKDNKEIELP"
     gene            3369..3938
                     /gene="nadD"
                     /locus_tag="PGA33_001649"
     CDS             3369..3938
                     /gene="nadD"
                     /locus_tag="PGA33_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HDG6473457.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHHDFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            3928..4512
                     /gene="yqeK"
                     /locus_tag="PGA33_001650"
     CDS             3928..4512
                     /gene="yqeK"
                     /locus_tag="PGA33_001650"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDG6473458.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            4513..4866
                     /gene="rsfS"
                     /locus_tag="PGA33_001651"
     CDS             4513..4866
                     /gene="rsfS"
                     /locus_tag="PGA33_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDG6473459.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYSQVAY"
     gene            4869..5585
                     /locus_tag="PGA33_001652"
     CDS             4869..5585
                     /locus_tag="PGA33_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDG6473460.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            5625..6311
                     /locus_tag="PGA33_001653"
     CDS             5625..6311
                     /locus_tag="PGA33_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDG6473461.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYT
                     SRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKSYFTT"
     gene            6403..6864
                     /locus_tag="PGA33_001654"
     CDS             6403..6864
                     /locus_tag="PGA33_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDG6473462.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            6869..9070
                     /locus_tag="PGA33_001655"
     CDS             6869..9070
                     /locus_tag="PGA33_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDG6473463.1"
                     /translation="MLFVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPIS
                     LFLIIFSAWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTDKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEVNEQFKERVKEIVIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLVITKQIKI
                     KSIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPSFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIVFIISVYYIFWLLAKRKYILVTFW
                     TIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYS
                     ISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKGYSSNTL
                     MLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKK
                     VLLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKN
                     NMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSSSYGNASGL"
     gene            9127..>9495
                     /locus_tag="PGA33_001656"
     CDS             9127..>9495
                     /locus_tag="PGA33_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDG6473464.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLK"
BASE COUNT         3448 a         1196 c         1756 g         3095 t
ORIGIN      
        1 acatgttaaa ttttatactg gcattttaaa agatagagaa gtagtgatta cccaaagtgg
       61 cattggaaaa gttaatgctg caatttctac gacattatta attaataagt ttaaaccgga
      121 cgtcattatt aatacaggtt ctgctggagc tttagatgaa agtttaaatg taggtgacgt
      181 tcttataagt gatgatgtaa aatatcatga tgcagacgca acagcatttg gttatgaata
      241 tggacaaata ccacagatgc cggtagcatt tcaatcaagt aaacctttaa tagaaaaagt
      301 atctcaagtt gtacaacaac aacaattaac agctaaagta ggcttaattg taagtggtga
      361 tagctttatc ggtagtgttg aacaacgcca aaaaattaaa aaagcatttc caaatgcgat
      421 ggcggttgaa atggaagcaa ctgcaattgc acaaacatgt tatcaattta atgtaccatt
      481 tgttgtagtt cgtgcagttt cagacttagc aaatggagaa gcggaaatga gcttcgaagc
      541 atttttagaa aaagcagctg tatcatcaag tcaaactgtt gaagcattag tgtctcaatt
      601 ataaaaaggt aaaggtgagt taaatgggtt tagttcgcaa gttttttatg ccgaattcat
      661 atgttcaatc aatatttcaa attgatttag acaagttagt ggacaaaggc gttaaaggta
      721 ttattacaga tttagataat acgctagtag gttgggatgt taaagaacct acagaacgtg
      781 ttaaagcatg gtttaaggaa gctaatgaaa aaggaatcac tattacaatc gtgtctaata
      841 ataatgagtc tcgtgttgct agttttagtc agcatttaga catcgatttt atttttaaag
      901 cgagaaagcc aatggggaaa gcgtttgata aagcaataac taagatgaat atcagaccag
      961 atcaaactgt tgttataggt gaccaaatgc ttactgatgt atttggtggt aatcgtcgag
     1021 gtctatatac aattatggtt gttccagtta aacgaactga tggctttatt actaagttta
     1081 atagattaat tgaaaggcga ttattacgtc atttcagtaa aaaaggttat atcacatggg
     1141 aggaaaattg attgtctgac attttaaaat gtatcggttg tggtgcgcca cttcaatctg
     1201 aagataaaaa taaacctggt tttgtaccag agcataatat gtttcgtgat gacgtgattt
     1261 gcagacgttg tttccgcttg aaaaattata acgaagttca agatgtagga ttagaaagtg
     1321 aagacttttt aaaattatta tcaggacttg cggataaaaa gggtattgtc gtcaatgtcg
     1381 tggatgtatt tgactttgaa ggatcattta ttaatgcagt taaacgtatt gtcggaaaca
     1441 aaaaaatcat tttagcagca aataaattgg atttattgcc gaaacaaatt aataaacgtc
     1501 gagttaaaga atggttaaaa cgaacagcaa gaaaatatgg tttggaagct gacgatgtcg
     1561 tattaatttc agctgaaaaa ggctggggca tagacgactt attatcatca attgcgaata
     1621 ttcgagaaaa tgaagatgtg tatattgtag ggacaacgaa tgttgggaaa tctacattga
     1681 ttaataaact gattgaagct agtgttggtg aaaaagatgt agtaacaact tcaagattcc
     1741 ctggaacaac tttagatatg atagatattc ctttagatga aacatcattt atgtatgata
     1801 caccaggtat tattcaagat caccaaatga cgcatttagt tagtgaaaaa gaattgaaaa
     1861 ttattatgcc taagaaagaa ataaaacaac gcgtatatca attaaatgag gcgcagacat
     1921 tattcttcgg cggtctagcg cgcatagatt atgtatcagg tggtaaacgt ccgttagttt
     1981 gtttcttttc taatgacttg aatatacatc gtactaaaac ggagaaggct aatgatttat
     2041 ggcgtaatca acttggcgat ttattaacgc cacctggaaa tccacaaaat tttgatctta
     2101 atgaggtaaa ggctgttaga cttgaaacag gcaaagagaa acgcgatgtt atgatctctg
     2161 gtctaggctt tataactata ggaccagggg ctaaagtaat cgttcgtgtt cctaaaaatg
     2221 ttgatgttgt attaagaaat tctattttat aaggtgatta aaaaaatgaa atttgcagtt
     2281 ataggaaatc ctatttcaca ttccttgtcg cccgttatgc atagagcaaa ttttaattct
     2341 ttaggattag atgatactta tgaagcttta aatattccaa ttgaagattt tcatttaatt
     2401 aaagaaatta tttcgaaaaa agaattagaa ggctttaata tcacaattcc tcataaagaa
     2461 cgtatcatac cgtatttaga ttatgttgat gaacaagcga ttaatgcagg tgcagttaac
     2521 actgttttga taaaagatgg caagtggata gggtataata cagatggtat tggttatgtt
     2581 aaaggattgc acagcgttta tccagattta gaaaatgcat acattttaat tttgggcgca
     2641 ggtggtgcaa gtaaaggtat tgcttatgaa ttagcaaaat ttgtaaagcc caaattaact
     2701 gttgcgaata gaacgatggc tcgttttgaa tcttggaatt taaatataaa ccaaatttca
     2761 ttagcagatg ctgaaaagta tttagctgaa ttcgatatcg ttattaatac aacaccagcg
     2821 ggtatggctg gaaataacga aagtattatt aatttaaagc atctttctcc caatacttta
     2881 atgagtgata ttgtttatat accgtataaa acacctattt tagaggaagc agagcgcaag
     2941 ggaaaccata tttataatgg cttagatatg tttgtttacc agggtgcgga aagctttaaa
     3001 atttggacta ataaagatgc tgatattaat tctatgaaaa cagcagtttt acaacaatta
     3061 aaaggagaat aacatatgct tactggcaaa caaaaaagat acttaagaag tttagcacac
     3121 aatattgatc cgatttttca aattggaaaa ggcggtatca acgaaaatat gattaaacaa
     3181 atagatgata cgttagaaaa cagagaattg attaaagtac atgtactaca aaataacttt
     3241 gatgataaaa aagaattagc tgaaacatta agcgaagcta ctcatagtga attagtgcaa
     3301 gtgattggat ctatgatagt gatttataga gaatctaaag ataataaaga aattgaattg
     3361 ccataataat gaaaaagata gtactttacg gcggtcagtt taaccctatc catactgcac
     3421 atatgatagt agctagcgaa gtatttcatg aattacagcc agatgaattt tattttttac
     3481 ctagttttat gtctccattg aaaaagcacc atgattttat agacgttcag cacagattaa
     3541 caatgataca gatgattatc gacgagcttg gttttggaga tatttgtgac gatgaaatta
     3601 aacgtggtgg tcaaagttat acctatgaca cgatcaaggc attcaaggag caacacaaag
     3661 acagtgagtt gtactttgtt attgggacgg atcagtataa ccaactagag aaatggtatc
     3721 aaattgaata cttaaaagaa atggttactt ttgtagttgt aaatcgagac aaaaatagtc
     3781 aaaatgttga aaatgctatg attgcaattc agatacctag ggtagatata agttcgacaa
     3841 tgattcgaca aagagttagt gaagggaaat ctatccaagt tcttgttcct aaatccgttg
     3901 aaaactatat taagggggaa ggattatatg aacattgaaa aagcaaaacg gcttgcaaaa
     3961 gagaaactgc cagagaaacg ttataatcat tctttaagag ttgcagaaac agcgattaaa
     4021 ttggcagaaa tttatgatgg agacactagt aaagtagaat tagcaggtgt attacatgat
     4081 ttctgtaaat atgatgattt aggtaaaatg tatcaaattg ttcgacaata cgaattaggt
     4141 aatgatctac tgagttatgg tagtgaaata ttgcatggcc ctgtgtgtgc agcaatcatg
     4201 gaacatgaat atggtatcaa tgatgaggaa gtattaatgg ctatcaaata ccatactact
     4261 ggacgtcaac aaatgacaaa aactgaaaaa ctgattttta ttgcagatta catcgaacct
     4321 ggaagaacaa tcccaggagt tgatgatatt cgagatatgg catacaatca aggtagttta
     4381 gataagacaa tttatgaaat ttctaaacgc acagtactat ttttaataca gaaagatatt
     4441 acggtatata ataagacgat tgactgttta aattattata actatagtga cgaaagaata
     4501 aaggatgatt aaatgaattc acaagaatta ttagcaattg ctgtggatgc aattgacaat
     4561 aaaaaaggcg aagatacgat ttctttagaa atgaaaggta tcagcgatat gacagattat
     4621 tttgttgtaa cgcacggaaa taatgaacga caagttcaag cgattgctag agcggtgaaa
     4681 gaagtagcca atgaacaaaa tatagaagta aaacgtatgg aaggatacaa tgaagcgcgt
     4741 tggatattaa ttgacttagc tgatgttgtg gtacatgttt tccataaaga cgaaagaaat
     4801 tattataata ttgaaaagtt atatcaagat gcaccattag aatcatatag tcaggttgcg
     4861 tattaattat gtcgcaatat gcagaaatga gcctagtgta cgatcaattg actcaagatc
     4921 aaccatatga aaaatggttt gaaattgtaa aaaatcactg caaagatgaa tcaaatattt
     4981 tagatattgg atgcggtact ggtagtttaa cagttcaatt agaagcttta ggtaatgtta
     5041 ctggtatgga tttaagtgtt gatatgttga ctgttgcagc tcaaaaatca gcaaatgtaa
     5101 attggcttga aggagatatg acatcttttg atttgcaaca acaatttgat atcatcacta
     5161 ttttttgtga ctctctaaat tatttgcaag atgaaactgc agtgattgaa acattcatca
     5221 atgtttatca tcatctgact gattcaggtg tatttatttt tgatgtacat actgtttata
     5281 aaatgatgac tttgtttaat aatcaaagtt atattgatga taaaggggac atttttttag
     5341 catgggatgc tgtgcaagga gatttacctt taagtgttta tcatgatatg acatttttca
     5401 ttcgacatga agatgaaaca tattcaagat ttgatgagtc tcattttcag agaacatttg
     5461 atgaaaagac atatttgtct tggttagctc aagttggttt taaacatgtt gaaacattta
     5521 ctgattttaa tatagatgaa cataatgaag atgcagaaag attgtttttc attgcgaaaa
     5581 aataaaagta aaaccactcg ctcagacgtg tataaagttg aggagtggtt ttattgtatc
     5641 aatttttatt acgctataaa gattttttaa ctcaatggaa gttatatatt ctaagtgctg
     5701 ttgttttaat tatggtatta attggtttta tattctggag acaagatgat tatacttcaa
     5761 gaaattttga aaataaagat actgctctga aacaaagcac tagtgaaaat agtagtttgt
     5821 ccaaagtaga agatgtccag gtcaaagatg gagataattc caaaaataag ggtcctgtat
     5881 atgtcgatgt aaaaggtgct gttaaacatc ctaatgttta taaaatgaca tctaaggata
     5941 gagtagttga tttacttgat aaagcacaat tattggagga tgcagatgta agtcaaatta
     6001 atttgtctga aaaattaaca gatcaaaaaa tgattttcat acctcataaa ggacaaaaga
     6061 atgttgaacc acaaattgaa gtaaacagtg tgcacgaaaa aaatgggaac acaaataata
     6121 ctaaagtaaa tttaaatacg gcatctgtat cagaattgat gtctgttcct ggagttgggc
     6181 aagctaaagc taatgcaatt gttgaatatc gcaaccaaca aggtgcattt caagaaattg
     6241 acgatttgaa aaaagtaaaa ggttttggaa gtaaaacttt tgataaactg aaatcttatt
     6301 tcacgacata atggattatt ttaattgcaa attttatttt ttgtgattaa tcttatatta
     6361 ttagaaaatg ttactaaatt ttaagtatgg aaggtgaaca tcttggaaag aatcaaatgg
     6421 gaagaatatt ttatggcaca aagtcatttg ctagcattac gttcaacttg tcaaagatta
     6481 tctgtaggtg caacgattgt taaggataat cgtattattg ctggtggtta taatggctct
     6541 gtagctggcg aggtgcattg tatagatgaa ggatgtttaa ttgaagatgg acattgtatc
     6601 agaacgatac atgcagaaat gaatgcttta ttacaatgtg caaaacaagg tgtatctact
     6661 gaaggtgcaa caatctatgt tactcatttt ccatgcctaa attgtacaaa gtcaattatt
     6721 caagcaggta taaagcgtat ctactatgca gaagattatc ataaccatga atatgcaact
     6781 aaattactca aacaatctgg tattgaattt aaaaaaattc cattttcacc agaatatgtt
     6841 gctaaatatc tgactaaagg ttaatgtttt gctgtttgtc gcgttatcaa tgattgtagg
     6901 agtgctttgg aattctagca aagtgctctc tacatttctt ttcattttac ttttgtatat
     6961 tacttatcgt aaaaataaaa tcgtttatgc ccctatttct ctctttttaa tcattttctc
     7021 cgcatggtat ttacattatt cacaacaagc aatatttaat tatatcaatt atattgaacg
     7081 taattctcag tttaatgagc gtgctcaagt aatccaaatt caacgtcaag gtagtgacac
     7141 atataaaggt aggttgagtt taaaaaatga aatatatcct ttctttttaa cagataaaaa
     7201 gaattttgat ttaaagaaaa ttgaaagtcg taattgtatt gttaaaggac aattcaaagt
     7261 taatgacaat aagtttgtaa ctcttaaatt acaaagtata gttgtacaaa gctgcctaga
     7321 atcgaaccgg tctaatttaa ttgagaaaca taaacagttt ataatgaatc gaatttatga
     7381 ttcgggtatt aagtttccgg atcgtattat ggcattgatt actggtgacg taaaagaagt
     7441 taatgagcaa tttaaggaac gtgttaaaga gatagttata tatcatttgc tggcagttag
     7501 tggctcgcat atagctgcaa ttgtattttt aatttaccaa cctttaaaac gattaaattt
     7561 acctttattt gtcattaaag gaattacaat cattgtatta gctttatttg ctcaatacac
     7621 aaattatgca cctagtgctg taagagctat aataatgaca actcttgtac tggttattac
     7681 taagcaaatt aaaataaaga gtattcagct attagcattt gcatttataa ttatgtttat
     7741 tttaaatcca ctagttgttt atgatattgg atttcaattt tcattcatca tttcattttt
     7801 tattatgcta ctttttcctt ttttacagca attgtcaaag ttacaatcat tattcataat
     7861 tacgtttatt gcacaattag cttcatttat cgttgccatt ccaagctttc atcaacttca
     7921 atgggtggga tttttatcta atttgatttt tgtaccgtac tattcgatta tattgtttcc
     7981 gctatctatt ttattcttta ttacaagtca ttttattgtg ggattaacgc cgctaaatta
     8041 cttggttgac ctaagtttta attttcatga ctggttacta gacctattca caagaatcaa
     8101 gcaatcacat ttttctgttc ccaagtttaa tgattggata tttatagtat ttataatttc
     8161 tgtttattac atattttggt tattggctaa acgtaaatat atattggtta cgttttggac
     8221 tataattatt ctgacattat taataacgtt tccaacaaat tcacatcaca aaattacaat
     8281 gttaaatgtg gggcagggag acagtatttt atatgaaggc ggtaagaacc aaaatgtctt
     8341 gattgataca ggtgggaaag tgattgatga tactaaacaa cctagttatt caatttctaa
     8401 atatcatatt ttaccaacgc taaatgaaag agggataaat gaattagagt atctaatttt
     8461 aacacatcca cacaatgacc atattggtga agtggaatat attattagtc atattaaaat
     8521 taaacatata gtgatataca ataagggata tagtagtaat acattgatgt tattatcgaa
     8581 attaagccat aagtacaaca ttaaacttat ggatgtaaga caagttagta gttttaaact
     8641 tggagatagt agttttctat tttttgatag ttttattcca aatagccgag ataaaaatga
     8701 gtattcgatt attactatga ttacatatca aaataaaaaa gttttattaa tgggcgatgc
     8761 tagtaaaaat aatgaatctt tactactaaa aaaatataac ttgccggaga ttgatatttt
     8821 aaaagtagga catcatggga gcaagacaag tagttctaaa gaatttatag agatgattaa
     8881 gcctaaaata agtttgattt cttctgggaa gaacaatatg tatcatcttc ctaatataga
     8941 agttgttaaa cgattgcaaa ggattcgcag tcgcatttac aatagtcaac aaaacggtca
     9001 agttacaatt gacttagatg ataatttaaa agttgattca agctcttatg gaaatgcaag
     9061 tggtttatag atacagaaac atgatataat ctattgatgt gtcaaacgaa tgaaagggtg
     9121 taatgaatga gcgacaatat tgtagctatt tatggagatg tgcctgaatt ggttgaaaaa
     9181 caaagtgcag aaatcatatc acaatttttg aaaagtgata gagatgactt taactttgtg
     9241 aaatataatt tatacgaaac agagattgca ccaattgttg aagaaacatt aacattgcct
     9301 ttcttttcag ataaaaaagc aattttggtt aaaaatgcat atatatttac aggtgaaaaa
     9361 gcgccaaaag atatggctca taatgtagat caattaatag aatttattga aaaatatgat
     9421 ggcgaaaatt tgattgtctt tgagatatat caaaataaac ttgatgaaag aaaaaagtta
     9481 actaaaactc taaaa
//
LOCUS       DAMQYT010000092         9330 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMQYT010000092 DAMQYT010000000
VERSION     DAMQYT010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9330
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.092"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            104..742
                     /locus_tag="PGA33_001657"
     CDS             104..742
                     /locus_tag="PGA33_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473465.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADVFKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            complement(988..2919)
                     /locus_tag="PGA33_001658"
     CDS             complement(988..2919)
                     /locus_tag="PGA33_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HDG6473466.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNK
                     DEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMHELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLDADNSINHE"
     gene            complement(2932..3579)
                     /locus_tag="PGA33_001659"
     CDS             complement(2932..3579)
                     /locus_tag="PGA33_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDG6473467.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVAIVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            3700..4251
                     /locus_tag="PGA33_001660"
     CDS             3700..4251
                     /locus_tag="PGA33_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDG6473468.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQN"
     gene            complement(4481..5689)
                     /locus_tag="PGA33_001661"
     CDS             complement(4481..5689)
                     /locus_tag="PGA33_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDG6473469.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
     gene            complement(5799..5927)
                     /locus_tag="PGA33_001662"
     CDS             complement(5799..5927)
                     /locus_tag="PGA33_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473470.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCGKIYEVLRFISFYTFYKL"
     gene            complement(5958..7340)
                     /locus_tag="PGA33_001663"
     CDS             complement(5958..7340)
                     /locus_tag="PGA33_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473471.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANISVTLKGDTKLNDSSK
                     KVTINDHEMAFSSSKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            complement(7580..8035)
                     /locus_tag="PGA33_001664"
     CDS             complement(7580..8035)
                     /locus_tag="PGA33_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDG6473472.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            8230..9198
                     /locus_tag="PGA33_001665"
     CDS             8230..9198
                     /locus_tag="PGA33_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDG6473473.1"
                     /translation="MLLNLLLIFILIVICPLIFNYTLTLNLTCYILVVISIFLSLIYS
                     IMTYTFPWSMFVTFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFI
                     STIQSPIVKGLSMWLAILCLSLLLTFICYLVWTVSYRHRSYNKEVDIIIVLGAGIFTE
                     FVTPMLAARLDRALDIYQQQASATKIIVSGGQGPDEPISEALAMQRYLIAHGVDKTDI
                     LMESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGS
                     HTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
BASE COUNT         3086 a         1633 c         1244 g         3367 t
ORIGIN      
        1 ttcaaattta agactacaat tgaacagtga tttttcatca ttataacaga caactagaca
       61 tattgataag taaagaaaag aactttatac ggaggtacct tgcatgacaa atccaaatca
      121 acgattagaa ccatttgatg agacatttca acaaccgaat attcatcgtg gtaagcgata
      181 tggtaagaaa aaacgttcat tggtaagcat gattattcaa atcattgttg ttatattaac
      241 cgcaatcgct ggatacagca tgtggaaaca acccattttt aatattgtct tcaccaaaga
      301 atctgtagat tttgatggca ttaaaaattt taacgagaca attggaaaaa tgcatcaatt
      361 aaatattaat ttgggtaata tcaatgattt acaacaagga atcgatcgct taattttagt
      421 ctttaacggt ttctttattt tatgtatcgt tagcctagtc ataacaatac tgacaattgt
      481 atttaatcgc acagttctaa aagtagtaaa tattttagtg ttaacaatta tgttagtcat
      541 tacattgtat ttcagttata ttattaaagc actcgcacaa gaaattgcag atgttttcaa
      601 aaaatattat ttaaatgtac caccagatca agtgattaca gaagctgacg ctattcataa
      661 cggcatcatt ttacttggct gtagcatcgg tttacttgtt ataagcttat tcttccgtaa
      721 tcgcatgcct cgtttaaaat aatgttatat agtagggatt gggacagaaa tgttattttc
      781 acaaaattca tttcgttgac ccacttcctg ctttttcttt attaatgacc tactacgtca
      841 atgccaaatt ttaatataat tcctctaaca ttctataaat caacaaataa aaaaagtatc
      901 gataaccata tttggcaccg atactttaaa aatcatgtcc ccacaacata atttttgatt
      961 agtcacactt caattaattt attttattta ttcatgattg atactattat ctgcatctaa
     1021 cttttgagct gtagcttttg cttttttatt actttgtaaa aataaacata aaattaaggc
     1081 gatgatgcta aatatcgttg caactataaa tgcatcatta ataccttcaa ccgttgctag
     1141 tttatttaca aattgtagta acactttcat tgcgccttct tgtccgccat attgtgatgc
     1201 taattcatgc atatgatctt gtacaacagg attcgtttta tctaactctt ccccaaaagc
     1261 tgataagtgt tgtgtagttt gtgttgtcat tacagtaact aagattgctg tacctataga
     1321 gcctgctaat tgacgcatcg tatttaagaa agcattacca tgagaggcaa gtcgtcccgg
     1381 taacgcatta atagctgcag ttaccattgg catcattata aatgccatac caaatgaacg
     1441 aagtacatag atacccatga ttgtcatata tggtgtatcc atatttaatt tagttaattc
     1501 ccatgttgca taagtcatta cagcaatacc aaagatagct aatggtttta aaccaatagt
     1561 atctaacaat ttacctgcaa atggtcctag tagacccata attagagaac caggtaataa
     1621 taacaatccg gaatctaatg ctgagaatcc gcgtaaattt tgtaaataaa tcggtaataa
     1681 aatcatacca ccatataaac ttaacattac aaccatatta ataattgttg ttaatgtaaa
     1741 tgttgggaat ttcaatactt ctaaattcaa cattggtgat ttcattctta attctctaat
     1801 aacgaataga ataataaaga taataccaat cgcaaacatt gtttctatct ctactgaacc
     1861 ccaacctttg ttgccagctt ctgagaaacc atataacaaa gcaccaaaac caatcgtact
     1921 aaaaatgata cctgggatat cagctttagg gtttgttgta tattgatata acttaaacca
     1981 tacaaaacca attaaaatag cgataatccc gataatgaac ataccgtaaa acatcacatt
     2041 ccaatggtaa ttttgtacaa tataacctga taatgttgga ccaattgcag gtgctaaaat
     2101 cattgcgata cccattgtac ccatggcagc accacgtttt tcaggtggat aaattgtaat
     2161 aataacaatt gaacctaatg gcattagtac acctgcacca atggcttgta atacacgtcc
     2221 aaccatcatg attgggaaat tcattgaaat cgcacagatt aatgaaccaa ttgtaaagag
     2281 tactaacgca actaaaaata attttcgata tgaatattta ttaaatagat acgccgtaat
     2341 tggtattaaa ataccgttta ctaacatgaa tcccgtcatc aaccattgcc ctgttgacgc
     2401 agaaatatta aattccgtat taatttttgg taaagcaaca tttaataatg tttggtttaa
     2461 aatcgcaata aacataccga ataataatgc cgctaatatt ttaccgcgtg aaacaccttc
     2521 accaaaaata aagtttttat gttctttttt tattttttca ttcactttat attcttctga
     2581 ttcaggattt ttagcagcaa ctgcttcctc atccttatta ttagtgaatg cttcttgatc
     2641 tttctcagac ccctttgtgt aaccatttag actaacttgg ctatgatcat cttgattgtc
     2701 aacacgcaac tcttcatgcg tcatacgttg tgtcgattga tcagttgttt tgtctaaatc
     2761 actagcttta aatttagatt gatttgattg acgtgtcgta aattgttgtt ccttttgttg
     2821 gcgtttgcct ttttttcttg atcttattaa aaataaattg ataaccccaa caataatgag
     2881 cgctaaaata atgtagctaa taatgaaggt cgtagtcatt taatgacccc cttaattttt
     2941 atggattttt acttcagcgt tcattccagg aacaacttgt ttagacggtt ctgattctag
     3001 agtgatttta acaggtatta cttgagaaac tttagtgtag ttaccatcac tatttgatga
     3061 tggcattaat gaaaagcttg cagcagttgc ttttccaata ctatcaactt tacctttaat
     3121 agaagctttt tgaccgtcaa tagtcacatc aacatcttta cctacttcaa catctttaat
     3181 atctttttcg tcaatatttg ctgttacata taaatcatct aaattgtatg cataagcgat
     3241 tgggttacca gcttgcacca ttgaaccttc cataccatct aatttggcaa ttgtaccttt
     3301 ttgaggcatt ttaagatcca tatctttcgt ttcgccatct tgaccttgta cagtaacaat
     3361 tgctactttg tcacctttat cgagtttgtc accttgttta acattaagtg atttaatttg
     3421 tccagatgca ggacttgcta ttttaatttg atcgccattt acttttgcat tatcagttgt
     3481 tacatagctt gttgttttat tccaaaaata aaagccagca atcccaattg ctaacagtac
     3541 aacaaccgta atgacattaa ttaatatcat ctttttcatg aaacatttcc tcctatttgc
     3601 ttttaaaatt cactttattt attatagaga catgataact aaaaaaacac caacaatttt
     3661 tataagagtg ttggttaaaa ttcacaaagg tgtaacaata tgaaacgaca agccaaaata
     3721 gaaattcaaa acgcacttgt tgatttgatg gctgaatatc cttttcaaga aatatccact
     3781 aaaatgattt gcgcgtattg taatattaat cgttctactt tttatgatta ttacaaagat
     3841 aaatttgatt tattggatac catcaactca aagcataagg aaaaattcca atttcttcta
     3901 agcgcgctac atcataattt cgaaaatatt aaacaggata agcttaaact ttataaattc
     3961 ttcattatta ttgctaagta tatcaagcac aatgagcagt ttttcaaaga cattttagtt
     4021 acctatccaa tgaaaacatt attcatagac tatataaatt tagcgagaga ttactatcaa
     4081 caaattatga atgactatag tactacagta accaataaac agctttatgt tacatatatc
     4141 ataggcggtc aagctggggt gtttattaat tggctttcga atggttgtaa tgaatcacct
     4201 gaagaagttg ctgacatttt attggctaat acgattaaac ttcaaaacta ataatacggt
     4261 ttcattctaa taaagaaatg ctataaaaca actagataaa gttaaaccaa atggatagat
     4321 aatagtacat atgtaacggt gctatcaata taaaaatgcc gagatttcca cataataatc
     4381 tcggcatttt tatttttgat gctaaattca taggtctatg aatagctcaa gttttgtttt
     4441 ttaatttttc aattcaatat aatagaagaa actatgttta tcatcgattt gttttaaata
     4501 ccttcatatt ataaatctgt aaaataatta atataactgc tgtagctata ataataatta
     4561 tgtaaggtat aggattatct tcacctttta cccccactaa tggcgatgca acaccaccaa
     4621 ataaaaactg tactaaacct aataaacttg atgaactccc tctaccacta ctactttcat
     4681 ccatagctat tgtaaaacca agtgtcgcaa cacctgtcac tggtgctatt aaaatgataa
     4741 atcctatcgc taaaatccaa aagttccaat gattaagcaa tacaatcgtc actaaaatta
     4801 caccgataat ctgaatcatc gtcatgcctc tcatcaattt ttgtgagtct ataaaatcaa
     4861 caaggtaccc agtaagttga cttgatataa tgagcgtaat accaattcct gcaaacatcc
     4921 aactaaattg tattgcagtc atgccataaa ttttttgaat aataaacgga gacgcagaaa
     4981 tataagtaaa taatattaca aatgtcatac cttgaatgag cattggtaat acaaaacgtg
     5041 gcgtcttcaa taatattttg aagtttttaa acattgtctt taatccacta cttgactcac
     5101 gatttgtcac tgttaatgat tcaggtactt ttaataaaga acctatgacc atgacgaagc
     5161 caaagatagt cagaatgaca aagaccatac gccagacaga ataatttaaa attatgcccc
     5221 ctattgttgg cgcaacaact ggtgcaatac cattaacaag catcaataat gccataaact
     5281 tagttagttc attaccacta tacatatcac ttgctatagc tcttgaaata actgctgctg
     5341 cgccacctgt cactccttga agaaatctta atgcaaccat cagccaaata ttatgtacaa
     5401 aaacaatacc taaacttgct aatgtaaaaa taatcatagc tataataagc ggctttctgc
     5461 gcccagttga atctgaaatt ggaccagcaa acaaattacc aaacgctaaa ccaatcataa
     5521 acatcgataa tgttaattgt gcgttagacg tcgttgtttg aaaatcatgt ctaatatctg
     5581 gtaatccagg caaaaacata tctatcgata atgctccaat ggccgtcaaa gatcctaaaa
     5641 taataataaa tatcggtgaa cgcttgttaa aatgcgttaa atcattcata tgactatctc
     5701 ctttatataa tgattagttt ataactatca cgaacagaaa ctaaatttaa atatgctctt
     5761 ttatatatta gcattagtca caagtttcat gaacatcatt acaacttata aaaggtataa
     5821 aatgatataa acctcagcac ttcataaatt tttccacaaa aaacaatccc ttcaaagtaa
     5881 ttcacactaa ttgtaaatac tttgaaagga tgttgtttgt attccatgac acatgcatct
     5941 tatttaagtt ttatactcta tttttctgat gtcttgatta attgcaagcg gtcttgcgag
     6001 ccttgttcaa gctcatattc tttatctact tgcttaccgt tttcatttat taatcttacg
     6061 gttgcggtaa tcttatcacc atgtttttca gcatctataa tttgtggtga actaatcatc
     6121 attaaacttc ctttagatac acgcttcttt acatcatcat aaaaagatga tccttttttt
     6181 atatatgatg atacaaaatc aaaatccgac tgattaaacg cagcattatt cgctaaagaa
     6241 tatccagcaa agaattctat caatttgttc ttcaagctgt tttcttcttt ttctttcttt
     6301 tcaacatagt cttcgatatc ttcactgtca aaattcaaag ttatctctgt attgtatttt
     6361 aaatcgctag ctttaatcgt cttcgtctgt gttgtaaatg ttttatcttt cgctttacct
     6421 gaagctgaaa tggtaatatc tttattttgt ggatatggac catacgtttt ggaacttgaa
     6481 aatgccattt catggtcatt tatagttact tttttagaac tatcatttaa ttttgtatcg
     6541 ccttttaaag ttacagatat atttgcttct tcaaaatctt cagtaacatc taccgtttca
     6601 gaattacttt gtctaaaatc aaattttaaa tggcctgcaa aatcaccgtt ctcagttgat
     6661 ttcatagctg gaattctata tgtccccggt ataaaattac ctattggcgt cactttattt
     6721 gcttcagcta taaccatctt tttcttacca ccagatttaa actcatattt tgttttttct
     6781 ttcggtttaa caattggttg cttggtaggt gcagtaaagc tcatattatc gaaaaagata
     6841 taacgtgtgc catttttact tacacgtaat atattttgac cagatctggt ttgaatataa
     6901 gaagctacgc tggtcttact cttattcaat ttatgtaccg tatttttaag gtcgctgaca
     6961 aattgcttaa gcccaacttc atctttgata tagttaatgt atacttttgc ttcttcagaa
     7021 tctactttat tatctttagt acttaataat gttgctactt tttgtttatc attattctca
     7081 atcgcattca ctaatatttt agtttgcgct tcaggtgaat tgaagtttct tagtaaaaag
     7141 aacaatataa taattaagat taaaatgaaa aaaccaattg cccaaggcac catttttctc
     7201 atcttgatgt tattagtttg tatatcttca tcggttgact ttctatataa tgcttgacga
     7261 ctatcaaatt tatgtccaca ttgtgtacat atctgtacat catcttgtgc ttgttgtccg
     7321 cacttcgggc aagatttcat actttcacct caattctgtt cctcttcaat tgttatatgt
     7381 atatcacaaa aattatttca ttattttacc attattattt tacaaatatt atcccaagaa
     7441 tacaattcaa gaaatgatta actttacttt agaattgtac cttattacat tatttatgaa
     7501 agtactctct caattataac taaaaagaag gtgttcaata catacatatt tagtcttata
     7561 tttgaacacc ttcaaaaacc tatttatgat tgctatatgt tttaatgcga tgattgatga
     7621 cttctaatac ttgccttaca ttttcaatat cttcagaatt taaatcatta gtaatatctt
     7681 gcgcaatatc tgtcataatc gcattacgtt ctttcaaata tgctctacct ttgtcagtta
     7741 aggttatgaa tttcaaacgt tgatcaatat ttaaatttgg tttatctaac ttaactaatt
     7801 tagcatcgat taacttttta attcgtcggc ttactgcggc cttatttaca ccttgtcttt
     7861 gcgtgatttc actaattgtc aaaggttctt tatttaacat acctaatacg ttagactgct
     7921 ctaatgaaat ttcatattca ttttgtaaat ctttcaacat ttttgcagtt aatgcgttaa
     7981 cgttatttat aaactgctct aaaaattgaa tatggtcttg taaatgtttt accataatta
     8041 tttctcccta attactattt ttataaagta tcctcagcaa tcattgtatc gcacatctaa
     8101 aaaacacaaa aatattgacc catatataat ataacaaatt aagatttaca tgttgctgct
     8161 ttctccttgc tttaataaat taaatcatct acatttatat ttaatattta ttataagcga
     8221 ggtgtcagta tgttattgaa tttattatta atatttatac ttatcgttat ttgtccgtta
     8281 attttcaact atactctgac gttaaattta acatgctaca tcttagttgt catatcgata
     8341 tttttgtcac tcatctatag catcatgacc tatactttcc cttggtctat gtttgtaaca
     8401 tttataataa tcagtattat catgctattc aaacatagat atttattttc tcatacttct
     8461 agtcgcttat ttattaaaaa attattatct ttttcatata aatttgtact ctacacatca
     8521 gcacttctat ttataagtac gattcagtca cctattgtga aaggtttgtc tatgtggtta
     8581 gctatattat gtctttccct gttactaacc tttatttgtt atctcgtttg gacagtatca
     8641 tatagacata gatcatacaa caaagaagtt gatatcataa ttgtgttagg tgctggtatt
     8701 tttaccgaat ttgtcacacc aatgcttgca gcgcgtttag atcgtgcact tgatatctat
     8761 caacaacaag catcagcaac aaagatcatt gtatctggcg gacaaggacc agacgaacca
     8821 atttctgaag ctttagctat gcaacgttat ttgattgcac atggtgttga taaaacagat
     8881 atactaatgg aatctcactc tataaataca tacacaaact ttttatattc taaacaaatc
     8941 atcaacaatt tgtatcatga agcagttaaa attgtttgtg taacaagtca gtttcatgta
     9001 ttgcgcgcct tacgactagc ccaaaaatta ggtttatcta tcgatggtgt cggaagtcat
     9061 acaccatatc actttttctt acatgtgctc atcaaagatt atctaggcgt aatgtatcag
     9121 tatagattgt tattaacgtt atattgttca agtagttggt ttatatgtct ttacgccgct
     9181 tttatttata atagttaatc cacaaacaaa ctattattat aggttgtttc taatattgcg
     9241 tcaaactatc tttaaatatt cgttaatcct tgattagaca attgttatat tgaaaataaa
     9301 taaactaaag agtctgggac attaatccct
//
LOCUS       DAMQYT010000093         9187 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMQYT010000093 DAMQYT010000000
VERSION     DAMQYT010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9187
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.093"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(287..1033)
                     /locus_tag="PGA33_001666"
     CDS             complement(287..1033)
                     /locus_tag="PGA33_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDG6473474.1"
                     /translation="MPIEYFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVESHS"
     gene            complement(1038..1511)
                     /gene="ytkD"
                     /locus_tag="PGA33_001667"
     CDS             complement(1038..1511)
                     /gene="ytkD"
                     /locus_tag="PGA33_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HDG6473475.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIYYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            1577..1834
                     /gene="yidD"
                     /locus_tag="PGA33_001668"
     CDS             1577..1834
                     /gene="yidD"
                     /locus_tag="PGA33_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDG6473476.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(1831..2832)
                     /gene="menC"
                     /locus_tag="PGA33_001669"
     CDS             complement(1831..2832)
                     /gene="menC"
                     /locus_tag="PGA33_001669"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDG6473477.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVADGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGAKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(2837..4315)
                     /gene="menE"
                     /locus_tag="PGA33_001670"
     CDS             complement(2837..4315)
                     /gene="menE"
                     /locus_tag="PGA33_001670"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDG6473478.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMKSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGRDITTNSLLDNTMGIQYETSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNVYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(4474..4929)
                     /locus_tag="PGA33_001671"
     CDS             complement(4474..4929)
                     /locus_tag="PGA33_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HDG6473479.1"
                     /translation="MKICLMLYLLIFLSSCSTNNKIVGQRFEIQQHNETIGSIYFSAD
                     YAHIRGIEKGTAKYFIDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYL
                     IENLDDEVFHLSALQDMNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
     gene            5232..5882
                     /locus_tag="PGA33_001672"
     CDS             5232..5882
                     /locus_tag="PGA33_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HDG6473480.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSVKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTNNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTE
                     EVPANENTENNQHEIDEITTKDQSDDDINTPNVAEDKSQDDLKDDLKEKQQSSNHHQS
                     TQPKTSPSTETNTQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            5963..6958
                     /locus_tag="PGA33_001673"
     CDS             5963..6958
                     /locus_tag="PGA33_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDG6473481.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVSYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDKSSVEKSESNEETAPVEPMPHSKPTTSEAPPSQNIHNEDSMYDASTE"
     gene            7034..7660
                     /locus_tag="PGA33_001674"
                     /pseudo
     CDS             7034..7660
                     /locus_tag="PGA33_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7701..8045
                     /locus_tag="PGA33_001675"
     CDS             7701..8045
                     /locus_tag="PGA33_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HDG6473482.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIENAEHTLFTPYM
                     LETLSSLGVKDSIVDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLKQYKNVEFND
                     KILINWRIIQEK"
     gene            8143..8694
                     /locus_tag="PGA33_001676"
     CDS             8143..8694
                     /locus_tag="PGA33_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473483.1"
                     /translation="MEKNEYIAKYNEYSQLLDATYSQAVAYLLSKYGAVTDDYYKEKS
                     YTRFLNGEIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISKFKYSYDVQKKENL
                     VYCDLIEHLILHAIITKESHGQFGVAGLCQMIKPTVIDWYIGEYNPKPAWMQATKARA
                     YLPGILVEKLLIKIDDMLKGIEI"
     gene            complement(8912..>9187)
                     /locus_tag="PGA33_001677"
     CDS             complement(8912..>9187)
                     /locus_tag="PGA33_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473484.1"
                     /translation="TDLMRDSNDNSLKNLHEMIYESDVNQFSVDLLMPKKQIEALVYN
                     FYEVNNINLSSGLSEKERNKLLNLISTKLEVSKVAAGLRLYNLGIHI"
BASE COUNT         3159 a         1670 c         1435 g         2923 t
ORIGIN      
        1 gaaccttttc tgaaccatac gcatagcgta tggttttctt tttacaatta aagagccaac
       61 cgttgttata gtctaacaat ggttggctcc tcttatttta tgtgctaaaa atttataggc
      121 aattttatta caacaatgta catttaaggt gaccttcatg ccaaaatcgc atcactcatt
      181 taatggaagc agcacgtctt catataaagt accgatccct aattcaacgc atgtagtacc
      241 acatcttcaa agcttgatag ttcccatgcg cacaccacgt ttcatactag ctatgcgact
      301 caacttggtt cataaactct ttaatataag tcaatgtttc aaccatcgct ggtggtcttg
      361 gcacatgtcc ttctgccatt tgataaaatg tttcatgcgt ggcacctttt aactctagtt
      421 ggtccgctaa ataatacgca tgatgaatac caacttgctg gtctttccct ccatgtacaa
      481 ttaatattgg cggactgttt tcattaatgt ttggaatcgc ttggcgtgcc tcatatgccg
      541 ctcgatcttt tttcggatga ccaatcattc ttcgtagcat gcctcttaaa tcgacacgtt
      601 cttcatacat taaatcaata tctgagacac caccccagat tgtataactt gttactggta
      661 agtcttgaaa tgtcaacaat ccttgtaaac cacctcgcga aaaaccaacc atgtggataa
      721 atgcatgtgg atatttatca tgtagcaacc ttaataattg cgtcacatca tttaaatcgc
      781 cacggtaaaa ttcgtctttg ccttcactcc cattgttacc tcggtagtat ggcccaatca
      841 ctaaagtttg actatctgaa aattgcatta atctacctgc gcgcacacgt cctacttgac
      901 ctttgccacc tcgcaaataa actacaatgc gatttacttc atgatgtggt gtcatcatta
      961 aagcttttac ttgtaagtca tctgacaaat atgtaatttc ttcgaattga tgcgtaaaat
     1021 attcaattgg cattcgttta cgtttgataa aacccaagtg attgcaccct ctctacgcat
     1081 tttaaaatgg tactatcttg cagtaagaaa ctccgttgtg cgagttcaat atcattgata
     1141 cagttaaaca acactggccc tgctgtttct aaataatcgt tcttgcttac caatgattca
     1201 acttcgataa aatatacatc ttttacaaaa tcagtttgat catgtgtttc aatggtatat
     1261 tgtgctatgt aataaatatt tttaactttg gcgcctgttt cttcatataa ttcacgtgta
     1321 actgcttcag cactactttc cccgcgttcc cttttaccac caggaaattc aatcccccgt
     1381 aaattatgtt tggtaaaaag caattgattt ttaaacgttg gaatagctag cacatgattg
     1441 ccatctgcta tctcattatc ctttttaaat gtcaaattaa cttgacgatt atctttatcc
     1501 ctaaacttca cgcgcatcac atccctacat tgtatgttaa tataatagtt aattactatc
     1561 gttggaggca ttaattatga aaaagatatt cttggcgatg attcattttt atcaacgttt
     1621 catttcgcca ctcactccac caacttgtcg tttttatcca acatgttcag agtacactag
     1681 agaagcgatt caataccacg gtgctttcaa aggcctttat ttaggtatcc gtcgtatttt
     1741 aaaatgtcat ccgcttcata aaggcggctt tgaccctgtt ccgttaaaaa aagacaagtc
     1801 agcaagcaag cattcacata aacataacca ttaatatggt tgtaattgag ttatatccac
     1861 taaagggggg cgaaattcga gtcgcccctc ttttaatatg cctgaatgcg ccaccacatc
     1921 ttgttcaaaa taataacctg ctggtgtaac atctcctgga taatcacctt tacgagcaag
     1981 catcgctgta aaatagcggc ttaaaccata ttcgtacatg ccgccaataa ccacttttgc
     2041 accatgactt ttcaaagtat caattgccgt ttgcacttta tcaatgccac ctagacgaaa
     2101 tggttttaat acaacaactt tcacattgta taattctatc aaattaatta tgtccaacaa
     2161 cgatgttgcc ttttcatcaa gggctattgg aggtattgtt ccatccgcta cttcatcaag
     2221 catggagata tctttaaatg gctcttcgat ataaagaacc tgttcacgcg ctaataactg
     2281 taactgtgtg aaatcttgac gatccaagga ctcatttgca tctataacca attgaaagtg
     2341 aaagtctaat tcccgtaaca ctctaatttg atgcatgatt tgaggcgtcc attttaattt
     2401 aattctggtc ggctttgttg cttttaatga ctctagttgt ttatttgata agccgctcgc
     2461 tgtcgctcca tatgctactg aaaatgaagg cagtacatga aacatttgat acaatgccat
     2521 gacaatagtt gcccttgcag caggcgtatt ttccaatgaa tctactaatt ttagtgctgc
     2581 ttcatacgtt tcaaatgatt tatttctatt atcttcgaac cattgctcaa ttacatgttt
     2641 cactgaggca attgtttcat gatcatacca atctgtttga aaagcgttac attccccgaa
     2701 atatgcattt cctttgtcat caatcaattc gataaacaaa caatcacgat gcgttaaagt
     2761 gactttcggt gttacaattt gtgacttaaa tggctcacta tatttataaa aatgcaaagc
     2821 tgtcaacttc atcaaatcat cctctataca acttatttct ttgtaattta cctgttgatg
     2881 tataaggtaa agtatcaacc ttttcaaagt gtttcggtac tttatatttc gctaaatgtt
     2941 gtgataaata tgcaatcaat tgtgcctttg aaatgtcact ttcactgaca aaatataatt
     3001 taggcacttg gccccaagta tcatcaggat gccctacaca tactgcgtca ctgatacctg
     3061 gaaattgctt cgctaccgtt tcaatttgat atggataaat attttcaccg ccactaataa
     3121 ttaaatcttt acgtcggtca taaatcatga cataaccttc atgatctatt tcagcaatgt
     3181 cacccgtatt aaaataacca ttttcaaacg tacccgttaa atctgttgga tacaaatata
     3241 cattcatcac attggcgcct ttaatcatta attctccatg accttcttta ttaggatttt
     3301 taatttttac gtcaacattg gcacttggca tccctacagt gtcaggacgt gcatgcaaca
     3361 tttccggtgt tgctgttaaa aattgcgaac atgtctcagt cataccaaat gaattataaa
     3421 ttggcaggtt atattgtaat gccgtctcta tcatagtggc agataattta gcaccgccga
     3481 gtaatatttt ttgtaaatta taaggttcat gtaaaccttg ttgcataagc caatttaaag
     3541 tttgtggcac aagcgaaatg tgcgtgattc gttcattttt aatcatcgtt aaaatttgtt
     3601 cggcattgaa tttatcaaca atgcgcacag taaaaccttc aataacagct cttaaaagta
     3661 cactgagacc cgaaatatga taaatcggca agacagatag ccaattagtg tcacgatcaa
     3721 atcccaagct ctctttacat ccgattgcac tggcataatg attacgaaac gtttgtggca
     3781 ccgctttttg agggcccgtt gtccctgatg taaacataat cgatgcaatg tcatctaaat
     3841 taaatgatgt atttaatatg ttggacggcg actctttcgg caccacagtt tcattcgatg
     3901 tttcatattg gatacccatt gtgttgtcca acaaactgtt cgttgtaata tcccttccag
     3961 cgaattcaat atcatccagc gatacaattt gaaaccctcg taattccagt ggcaaggtac
     4021 aaaaaatcaa ttgtacatcg attgacttca tctgattcgt catctcatta ggtgtcaacc
     4081 ttgtattaat catcgcaatt tcaatatttg ccaaccaaca tgcatgtatt aaaatgatcg
     4141 attgaatcga attatctatg tatagcccaa cacgagattg ttgataagcc ttgagtcttt
     4201 tagccaatag actcgcttca cagtataaat tttgataagt ataagattct tgaccgtctg
     4261 ttatcgcaat atgatgtcca ttttgttgtg cttgtttata taaccaaaag tccatgcgtt
     4321 attcctccaa aatcatttac attataatta taacgatttt atgacattct agcagtggtt
     4381 atgtttaaaa atataaaaaa gtagacgaat tgatgcattg atatgattgt tataatgctc
     4441 aatacatatc gttatatcat tcgtctacta ttatcagtta tttttattta attttagtgt
     4501 cattctgtca ttttgatgtg gtgatttacc cattgttgcc acatcatctg caatgtcaat
     4561 tggtatacgg ttcatgtctt gtaatgcact taaatggaat acttcatcat ctaaattttc
     4621 aatgagatat acataatatg ttaccttgtc ctttttatat tttaacgttt tccaaaagtc
     4681 cggcttgcaa ttcaatacat tatccggaat atattcaata aataagtaac gtttgctgcc
     4741 tactttgtct atgaaatatt ttgcagtgcc tttttctata cctcttatat gtgcatagtc
     4801 tgctgaaaag taaatactac ctattgtttc attatgttgt tgtatttcaa atcgttggcc
     4861 tactatttta ttatttgtgc tacaactact taaaaaaatc agtagatata gcattaaaca
     4921 tattttcatc cccttgaatt ttaaaaactt tttcaaaagc aacacctcta aaaataaata
     4981 caatatatta tagcaaatag aaattattat tttgttaaat tatgttgtca tgttcgtgtc
     5041 attttgagtc gtaatacaac tttacaatta ttaaattaac acttctacaa tccaaaaata
     5101 tttttaaagt tttgaaaaaa attaaagtat ttccactact aatttggtaa aatatgatta
     5161 tgtgatatag tgaaagcgtt ttacacttaa taactccctc ttaaatgcat ccaggtttta
     5221 tgtagtaaat catgaaaata acatataaat atagaggaga tttacctttg aatacagaga
     5281 acaacaagaa tcaaaaccaa tctgttaaaa attctgaaag acgcggcatg ttaaaaggat
     5341 gcggcggttg ccttatttct tttattttat taataatctt attatcagcc tgttcaatga
     5401 tgtttagtaa taatgacaat tccactaata atcaatcatc aaaaacgcaa ttaactcaaa
     5461 aagatgaaaa taaaaatgaa gataagcctg aggaaaaatc agaaacagca acagatgagg
     5521 atttacaatc aaccgaagaa gtacctgcaa atgaaaatac tgaaaataat caacatgaaa
     5581 ttgatgaaat aacaacaaaa gatcaatcag acgatgatat taacacacca aacgttgcag
     5641 aagataaatc acaagacgac ttgaaagatg atttaaaaga aaagcaacaa tcaagtaacc
     5701 atcatcaatc cacgcaacct aagacctcac catcaactga aacaaacacg caacaatcat
     5761 ttgctaattg taagcaactt agacaagtat atccgaatgg tgtcactgcc gatcatccag
     5821 catatcgacc acatttagat agagataaag ataaacgtgc atgtgaacct gataaatatt
     5881 aaacaacaag cgaattgaat tcaaattgta tttagcttta tgcactaatc acatagtaaa
     5941 taatgaggga gattttttag gcatgagcaa tcaattcaaa agcgaagaag agcgaagaca
     6001 atgggaacaa ttccaagctt tccaaaatca acaaaaccaa cagaaccagc aatacggaca
     6061 aaagaaatct aaaaaaggat ggttctgggg ctgtggtggt tgtctagtat tatttatttt
     6121 aattatcatc ggtatttcag cttgtacagc tggtattaca ggtaaccttg gcggaaatag
     6181 ttctaaagaa acgaacaaaa cccataaaat cggtgaaact gttaaaaatg gcgaccttga
     6241 agtcactgta aattcagtgg aaactatgaa atctgtagga ccatctcttg caccaacaaa
     6301 cgctaaaggt atatttgtcg ttgctgatgt gacgattaaa aacaaaggta aagaagcgtt
     6361 aacaattgat agttcaatgt ttaagctaaa atccggtgat aaaacatttg aagcagataa
     6421 tacaggttca atgtctgcta atcaaagtga caatggtagt atagaaaatt catttttctt
     6481 acagcgtata aatccagata gcactgctca aggtaaaatt gttttcgatg tgtcagaaaa
     6541 catagccaac gcaaaagata aaaaattaga agttatttct agtttattta gcgtcaagaa
     6601 gattacattt gatttatccg atgctaaaaa aacatcaaaa gctaaaaaag acaagcaaga
     6661 tacagaagta gctgttgcga gttcaaatag cgataatgta agttatgaag cttcggctac
     6721 tacacctgct acaacttcta gtgcggatac tgattctgaa gatagcgaaa agtctagtaa
     6781 agatgaggat aagcagaatg cgtctaaaag tgataaatct agtgtagaaa aaagtgaatc
     6841 taatgaggaa actgctcctg tagagcccat gccccatagc aaacctacca ctagtgaagc
     6901 accacctagc caaaatattc acaatgaaga tagcatgtac gacgcttcaa cagaataaaa
     6961 tttcagggta gctcggctac ccttctttta cggaaaaatt aattatacat aatcaaatca
     7021 aggagataaa aaaatgaaat tcaaagctat cgttgcaatc acattatcat tgtcactatt
     7081 aactgcctgt ggtgctaatc aacataaaga aaatagtagt aaatcaaatg acactaataa
     7141 aaagacgcaa caaactgaca acactacaca gtcaaataca gaaaagtaaa tgacaccaca
     7201 agaagccgaa gatatagttc gaaacgatta caaagcaaga ggcgttaatg aatatcaaac
     7261 attaaattat aaaacaaatc ttgaacgaag caatgaacat gaatattatg ttgaacatct
     7321 agtccgcgat gcagttggca caccattaaa acgttgtgct attgttaatc gacacaatgg
     7381 cacaattatt aatatttttg atgatatgtc agaaaaagac aaagaagaat ttgaagcatt
     7441 taaaaagaga agccctaaat acaatccagg tatgaacaat catgatgaaa cagatggtga
     7501 gtcagaagac attcaacatc atgacattga taataacaaa gcaattcaaa atgacatacc
     7561 agatcaaaaa gtcgacgata aaaatgataa aaatgctgtt aataaagaag aaaaacatga
     7621 taatggggca aataattctg aagaaactaa agttaaataa tggcatactt tgattaatcg
     7681 taatttttag gagaaacgct atgataacat ttgagaatat acaacaactt gagaaataca
     7741 ccttaatgac tatgcatggt ctttttaatc aacttaaact cggcattatt tcaatcgaaa
     7801 acgcagagca tacgcttttt acaccttata tgttggaaac gctctcttcc ctaggcgtga
     7861 aagacagcat tgtcgattta attcataaag ggactgaatt agaagacttt gcggcattta
     7921 atttatcaat tgaagacaca gttacagtct gtttacaaag aactgaagaa ctattaaaac
     7981 aatacaaaaa tgtggaattc aatgacaaaa tattaatcaa ttggcgtatt atacaagaga
     8041 aatagacata taaaagtcga atgtaactac gtgagtattg attttattct ttgtaaatta
     8101 caagcatttc atattataaa gtttgaaaag aggtatattg aaatggagaa aaatgaatat
     8161 atagctaaat ataatgaata tagtcaatta ttagacgcta catactcgca agctgtagca
     8221 taccttttaa gtaaatatgg cgctgtaacc gatgattatt ataaagaaaa atcatacacg
     8281 cgatttttaa atggagaaat caaaagtatt tcaaaaggaa aatacactag agctagcgaa
     8341 ggattatatt gccatcatat aagcgaggac aaattccaaa atctatctga tctaagattc
     8401 atttccaaat ttaagtactc atacgacgtt caaaagaaag aaaacttagt gtactgtgat
     8461 ctaatcgagc atttaatttt acatgcaatt attacaaaag aatcccatgg ccaatttggt
     8521 gtagctggat tatgtcaaat gatcaaacca acagtcattg attggtacat tggcgaatat
     8581 aatccaaaac cagcatggat gcaagccacc aaagcacgtg cctatttgcc tggaatatta
     8641 gtagagaaat tactcattaa aattgacgat atgttaaaag gaatagaaat ataagatttc
     8701 cttgagtcta gataaatgat taatgtagat ttattttttg ctgttgagat tttgttatag
     8761 atgtttaaac ctgtaattaa atatatttta taaaatagac cacgcatacc tatctataaa
     8821 cggacaatgt ttataaatga gtttgcatgg tcttgaattg tattaaattt cttttggttt
     8881 taataaatcg actagatttt cacaatattt atcaaatatg tattcctaaa ttatacagcc
     8941 ttaatccagc agctactttc gaaacttcca acttagttga tataaggttc aatagtttgt
     9001 ttcgttcttt ttcagataaa ccagaactta aattgatatt attgacttca taaaaattat
     9061 agactaatgc ctctatttgc tttttaggca taagtaagtc gactgaaaac tgatttacgt
     9121 cgctttcata aatcatttca tgtaaattct ttagactatt atcgttacta tctctcatta
     9181 agtctgt
//
LOCUS       DAMQYT010000094         9050 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMQYT010000094 DAMQYT010000000
VERSION     DAMQYT010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9050
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.094"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(101..211)
                     /locus_tag="PGA33_001678"
     CDS             complement(101..211)
                     /locus_tag="PGA33_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473485.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVEREKWYFSGKEV"
     gene            complement(311..847)
                     /gene="nrdG"
                     /locus_tag="PGA33_001679"
     CDS             complement(311..847)
                     /gene="nrdG"
                     /locus_tag="PGA33_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDG6473486.1"
                     /translation="MILLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            complement(844..2694)
                     /gene="nrdD"
                     /locus_tag="PGA33_001680"
     CDS             complement(844..2694)
                     /gene="nrdD"
                     /locus_tag="PGA33_001680"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDG6473487.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            complement(2941..4311)
                     /locus_tag="PGA33_001681"
     CDS             complement(2941..4311)
                     /locus_tag="PGA33_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HDG6473488.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            complement(4633..4728)
                     /locus_tag="PGA33_001682"
     CDS             complement(4633..4728)
                     /locus_tag="PGA33_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473489.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKDT"
     gene            complement(4804..5409)
                     /locus_tag="PGA33_001683"
     CDS             complement(4804..5409)
                     /locus_tag="PGA33_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HDG6473490.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGVVASRRAQTLNQYVEHMTVISPTI
                     TEKLQNMVDNGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKKALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKVLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR"
     gene            complement(5472..7352)
                     /locus_tag="PGA33_001684"
     CDS             complement(5472..7352)
                     /locus_tag="PGA33_001684"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HDG6473491.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEED
                     AEKWMADIINIIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLIFMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESKRF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRD
                     VY"
     gene            7547..7639
                     /locus_tag="PGA33_001685"
     CDS             7547..7639
                     /locus_tag="PGA33_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473492.1"
                     /translation="MSVINAYSNASLLFFIYLLFIMYQISLSVK"
     gene            complement(7747..8244)
                     /locus_tag="PGA33_001686"
     CDS             complement(7747..8244)
                     /locus_tag="PGA33_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDG6473493.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(8254..8514)
                     /locus_tag="PGA33_001687"
     CDS             complement(8254..8514)
                     /locus_tag="PGA33_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473494.1"
                     /translation="MIIMRGYNGRYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
     gene            complement(8896..9009)
                     /locus_tag="PGA33_001688"
     CDS             complement(8896..9009)
                     /locus_tag="PGA33_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473495.1"
                     /translation="MNGILIKCLNIGGQYYLQTRMLSNVDNAEKIISRNSI"
BASE COUNT         2803 a         1724 c         1251 g         3272 t
ORIGIN      
        1 ttacgattta acgaatttaa caaattttac tagaatggca tttaagaata tttatacgtt
       61 attaacgaat atttatttat tgtaaaacgc taccaaaaag ttagacttcc ttcccactaa
      121 aataccactt ttctctttca acttttttaa aaaacggata tgcaactttt agtattggta
      181 tcaaaatgat tgttaggtca tattctatca atatattttt ataaagaatt gcttttatta
      241 actttcaatt atgtacctaa cctaaaaaga agccaaggca acgaatgtta ccttgacttc
      301 taatacatat tcaactaact atatattcaa tcatacgcgc atgcgagagt gattgttgta
      361 catctataat gcgttgattt aaagaacctt tatatggtaa atcaggtttg aataagtgtt
      421 gtataaatag accatctact aaaacgtcaa tgtatgataa taactctcga cgttctgtac
      481 aatcatttgc taaatattca tataaaaatc cagtccatac ccaaattgtc tttgtatttc
      541 caaaacgtgc tcgaaatgct ttgacaagat ttaatgtaat atccaaatta caaaatggtt
      601 cgccacctaa tagacttagc ccagatatat aatcatgatc gcaatcatct aatatttctg
      661 ctaatatttc atcagtgtat ttctcgccat atctgaactt ttgtgaggct ttgttataac
      721 atccaacaca attaaatgga catcctgata cataaacact gcatcttact ccttcaccgt
      781 caacaaagct atttgattct attttagcaa tataaccttg tccttgttta atgtctaaaa
      841 gtatcattct ttaggcgctt tcatatgttt tactcgtgcg caaatttctt tatgacggcc
      901 tttaattact ggacgttgaa ctggattgcc taggtaacca catgttcgtt taacgacatc
      961 aactgtttta ggattatcat tgccacagtt cgggcattta aatccttttt cagttgcttc
     1021 aaaatctcca tcgtaatcac attcataaca atgatcaatc ggaatatttg tacctaagta
     1081 accaactttg tcataagagt agtcccatac cgcttctagt gctttcaaat tgtgttgcaa
     1141 tttcggatac tcacaatagt gaatgaaacc accactcgca taataaggat aatctttttc
     1201 aaaatctaac ttttcaaaag gtgtaacatc tttacgtaca tcataatgga aagagttttg
     1261 ataatatcct ttatctgtaa tgtctttaat atctccaaat ctctcttggt ctaaacgaca
     1321 aaaacgatcc gttagcgatt cactcggcgt actgtaaata ctgaaccaaa tgtcatataa
     1381 ttctgtccat ttcgtttgat aacgtttcat ttctttaaga atttcaagcg taaatgcttt
     1441 tgcttcttga gatgtttccc agtctggacc atagaaaaca gtagctgttt catacaaccc
     1501 tatatagccc attgaaatcg ttgcacgttt atttttaaat aactcagcaa catcatctgt
     1561 ttcttttaat ttatagttaa atgcgccact tttatataaa atcggtgcgt tattcggtac
     1621 agcatctttc aaacgattta tacgataaag taatgcatca tgtaacacat cgatacgttc
     1681 ataaaagatt tcccagaatt tcgtcatatt accggcagat tctaatgcca ttctaggtaa
     1741 attaagtgta acaacaccaa gattacaacg accattattt tcaaaatgac cttccgcatc
     1801 tttccaactt ggtaaaaatg aacgacaacc cattggcgct ttgaaatcac ctaatatttc
     1861 tacgagtttg tcataattta aaatatctgg atacatacgt ttcgttgaac actttaatgc
     1921 tagttgttta atgtcatagt tcggatcttg cggactaaag ttggttcctt ttttaattga
     1981 aaatacaagt ttcgggaaaa tcgctgtcgt gcggtctttt cctaagcctt tgatacgagt
     2041 atttaagata gcttgttgaa ttttgcgact taaatgatct gtacctaagc cgaatcctaa
     2101 tgttacaaaa ggtgtctgtc cattagatgt atataaggta ttaatttcat attctaaact
     2161 ttcaatcgca tcattgatgt ctttagtgac ttgttgatca acataacgat caatttcaga
     2221 ttctttgaca aattgctttg cgatattcct atgttgttct tcattatgtc gtgcatatgt
     2281 actaagtaat tcgtcaacgc ggtcaaccgt acagccacca tattgactgc tagaaacatt
     2341 ggctataatt tgtacaagct gcgctgatgc agtttgtatt gattttggtg aagttacatt
     2401 cgcgttgcct atttcaaatc cattatgtag catattttta gcatctatta aacaacagtt
     2461 agttaacggt tggaatggat gataatctaa gtcgtgaaaa tgtatgtccc ctctttgatg
     2521 tgcatctgca acgtgctttg gtaataaatg atttaatgcg taagatttag aaacgatacc
     2581 tgctgttaaa tctctcattg ttgaaaatgt atcactatct ttgttagcat tttcgttaac
     2641 aatagcagga tctttagaaa tcaaacccgt taatgcttct tcgatttgat tcattttacc
     2701 ccttcttcct atatattgtg tctactcatt acgttatacc actatatata gtattttcaa
     2761 atttttatta gtctgttaac taaaacgtca caaagttatt aacatttagt gaaattcata
     2821 tttctacaca attatttcat taatttttaa aattgtttaa agattcgtcc gtcatttcga
     2881 catgtttatg caactattat ttcattttgc aacgtgacca tagtttctat tttttcatac
     2941 ttaaatcgtc acaatgccca tcaacattgc aattactaac ataacgatag cgaatcccca
     3001 aatccaaaag aatgcatact taatatacgt gcccatgttt gcctctgcta aaccaattgc
     3061 caaccataag gctggtgaaa atgggctgac aaatgtacct ataatattcc ctatgaccat
     3121 tgaataagct gttgatacag acggtacacc aaattgccct gctgtttgtt cgacaatcgg
     3181 taacacagca aaataataag cgtctgtgct agttagtaaa tctaatggta cgccaagtaa
     3241 acctacaata atatgcaagt atggtcctac ttctgcagga atcactttga ttaaattggt
     3301 cgcaatcgct ttaagcatac cggtttcatt tagtacacct aaaaacatac ctgctgcaat
     3361 aatcactgca gccatcatta atgcattcgg cgcatgcgct cttaatcgtt ccatttgttc
     3421 atccactgat ttaaaattaa taacaagtgc caacgaaaca cctatcatga atgcaaattc
     3481 aggtggcgca atatttatta acatcgatag aataacagct aaagttaaag ctgtattcac
     3541 ccattttatc catgattttg ttcttgcacg tccttttaca ggaaacctta catcttgatc
     3601 tcgttcatat acttcaacta atttatgtac atctatatct tgtgtttgcg gtaattcatt
     3661 tctctctatt gcttttttga tacgtttctg ttctttaaat ccaagatata ccgcaaacaa
     3721 cataacaaga atgaaaccta ttatttgaat aggtattaat ccataccata attcattgac
     3781 acttttggct tttaacactg cagctacacg agccattgga cctccccaag gtaccatgtt
     3841 cataatcgcc gcgcttaatg ctaatagtaa aatcaataaa tatttattca tatttaacgc
     3901 tttatataaa ggtaataatg caggaataga aagcaaaaat gttaccgcac cggccccatc
     3961 taattgggct attgtgccaa ttaaagctgt cattgcacag acaatgacga cattgcctcg
     4021 tgtcattaat attaagcgtt tgacaagcgg cttgaataaa ccactatcgt tcatgatgcc
     4081 aaagaaaata atggcaaaga taaacataat aacaacgttg atgacttgat ctaacccttt
     4141 agcaaaaaat ccaaccaaat ctgtcacact atatcctaaa atcattgccc ctaagcaagg
     4201 tatgattgtc atacctacaa ctggatttat ctttttggca atgagtaaac ctacaattga
     4261 aataataata atgagcccca ttactgttaa ccacatatta tcactattca tattattttc
     4321 tccctccact tttaatacaa tttacataat ccccctcttt aacttaaata ttataattaa
     4381 tataattttt tatatttaag ttatctcatt ttatcaaaaa aatctttttt gcgtaactat
     4441 caatagtgaa gtttgatttt cctacttgat aacatggcat gactaatcct tgtatatcct
     4501 gtcctgtatt tcattttgat ctatcatttt tattattgct ttctattgca caaaaaatct
     4561 ggctaaaggt cgctatgata gtaacgactt caaccagatt tcaatttatc cacctatttt
     4621 attaagtttg ttctacgtat ctttttctct attgtaatat tcatatttaa aacgcatatc
     4681 attatcgctt aaatccttca ttattagact attcacaaag ccgcccatgt cttaatttca
     4741 atgattaagc gttaaagtta aaataagaag acttacctta gacggttaga cggtccgcta
     4801 ttattatctt acatccaacc acgctaaaaa ttgagcttgt ttgtcatgat ttaaatattc
     4861 ttgtgacaca atttgtgaca gtaactgttg cttttcgtta tatgttatat caagtacttt
     4921 tattttctgt cggcaagtat ataaaaagtc gatatacgaa ctgtatgatg gtggatataa
     4981 cgcctcaagc tctgccataa ttgattttgt caacttagga ctcgcaccat cagttgatac
     5041 actgatagtt agcttgtcgc ggtgtagtgc acttggaaat acaacattgc catttgatgc
     5101 atctccaaca ttattaaaaa gggcatgctc aggtaaggct ttttttaccg cttcattgac
     5161 acgtggctca ttggttgctg caataactag atacgcgtct acaatatcgc ttggttcaaa
     5221 ttctttttct ttccatatga cgacaccgtt atctaccata ttttgaagtt tttcagtgat
     5281 tgtcggactg atgaccgtca tatgttcaac gtattgattt aatgtttgtg cccgacgact
     5341 tgcaacgacg cctccaccaa ctatgacgac atttttattt gttaaatcta tcattaatgg
     5401 catattcatg ttgagcaaca tcctttatat ctttttacat cattacacct taaaatattt
     5461 aacaccaatc gctaataaac atcacgttga tagcgttgtt gttgtttcat ttgtcgcaat
     5521 aataactctg cttcttcttg agaaatatga cgttctttta ccaatacatc tttaatggct
     5581 tgatggacat ctttcgccat acatttttca tcgccacaaa tatagattgc tgcgccttgc
     5641 tcaatccatt cattgaaacg tttactttct tccattatac gatgctgtac atattctttg
     5701 tgttcttggt ctcttgaaaa tgctaaatct acgcgtgtta agtttccatt ttcaagccat
     5761 tcttctattt cttcttcata taaaaagtca gaactacggt gttgatcacc aaagaacaac
     5821 catgtttttc cagtcatacc aagttcttca cgttcttgta aatatgctct aaaaggagca
     5881 attccagtac ctggtccaat cataataacc ggtatatctt gcttcatcgg aaatttgaag
     5941 ttcggatttt tcttcaaata aattggtact atatcgcctg gtttaattcg ctcagcaaaa
     6001 tgaaccgagc atacaccttt tctctcacgt ccatgtgctt gataacgaac cgtaccaacg
     6061 gtaatatgca cttcatctgg cgttgccata aaactactag aaatcgaata ctctcttggt
     6121 ggtaattttc taaggatttg atacatattc tcaggttgta attctatagt tggaaaatct
     6181 gttattaaat ctataaagtc ccgatttata acatattcac gcgcccatga ctcatcttga
     6241 atacgttcag ataattcttc attgtcaaaa tagatatctg catttttcaa taatggcaat
     6301 gttaatttag taaattcaaa atgtgatgtt agtgcttcaa caataggaac tgtatcacca
     6361 tgatcattaa ttggcaccgg agattgcgga tcccaaccta acatgaatat tagtttttca
     6421 accaattcag ggtcgttttg cggtaatgct actatacaat ctcctggttc atatgattca
     6481 ctaaaatcat caagtaaaaa ttctatatgt cgtgtttctt tatttgaatc ggtaccattt
     6541 aaattgatat tcgctaatac ttctgcttgg tatggatttg atttagaata tttcttttct
     6601 ttggcagact taattgattc acttatcacc gattcacttt gaataccttc tgatgtggta
     6661 tcaataatat taatgatgtc tgccatccac ttttctgcgt cttcttcata atcaatatca
     6721 caatctacac gcttacatat acgctcagcg cctagatttt ctagtaaaac atctacatct
     6781 ttaccggctt gacagaaaaa ttcatatgtt tgatcaccta aagctagtac tgaatatctc
     6841 acatgattta aattaggtgc gttatcgtct tctaaaaatt caaagaaatc ccatgcatta
     6901 tcaggcggtt ctccttcacc atgagtagac gtaataataa ataaatcttc taactgcgcg
     6961 atgtttgtcg tatcatattc atccattgac atcaaaacaa cttgatgtcc gatatcactt
     7021 aaacgttctg aaaaaatttc agctaaacgc atggcattac ctgattcaga accatatagc
     7081 actgtaacat gccgctgatt agcttcgata tgtggctctt tattttgtaa catatacgtt
     7141 gttgaagttg cttgttcttc tgatatttgt gattgttgcc acacttctgt ctctaattgt
     7201 tgttgattac tatcacttgt cgtatcgtta ctattagcta gtaaaaaacc actaagccac
     7261 tgttgttggc tctctgttaa tgtttgcagc agattgttaa tctctgtgac ttgtttttct
     7321 gtaaatggac tattagatgt atttaatttc aaatgttttc acctcaataa acttgaaata
     7381 gatgattgac tcttttttta tcgatagttt agtcaatttt ttaatatgta tggtataaat
     7441 taaaacaaac ttgagttgtg tctgatagtt atacttgcaa atatttacaa ccttcatgtt
     7501 gcttgaatga cattatcagt aattcagaca aaactttgat tatgtcgtgt cagttattaa
     7561 tgcatacagt aatgcatcgt tgttattttt tatttattta ctatttatta tgtaccaaat
     7621 cagcctttca gtaaaatagg tcaaattact gattttctaa atataaaatg cctcctaata
     7681 acatactatt agtacatcat taagaggctc ttgtgttatt tgcatactaa gcgctcaaat
     7741 ttaaatttaa gatgaagatt cttgcaataa tatttctata tttgtcgata tatccaatgg
     7801 atcttccatt ggcaagaatc gatttacaac atttccttgt cgatcgatta taaattttgt
     7861 gaaattccat tttattgggg acccaaagat tcctggttgt tcattcttta aatgcgtaaa
     7921 taacggatgt tcatcgttcc cgttcacaga tattttagct aacactggaa atgttacacc
     7981 aaatttctca cgactaattt tcaagatttc ttcattagaa cctggttgtc gattgtcaaa
     8041 attattattc ggaaaactca acactacaaa cccacgatcc ttatattttt gaaataaagt
     8101 ctctagtttt ttcaattgtt cgctatatat acattctgtt gcagtattaa caactaaaat
     8161 cactttacct ctaaatgctt ctaatttata agttaagcct ttataatcac ttacttcgat
     8221 atcatacaca tttctattat tcataagaca cccctacaca gcctttttta tattgaatat
     8281 gttcttttta gaatgttctg ataaaataag tgcgcgttta caccgtgaac acacattata
     8341 tagcgtgata catttttcga gcacacgata aataatgttc gagtttatgt tgttgctcaa
     8401 cctatccgat ttaccgtctt ttttcacctg tcttacttta ttaaaataat cacattgtat
     8461 agctgtatta gtccagctac aatatcttcc gttataaccc ctcattataa tcatccttat
     8521 tttctatttt taaaaagaca attagaccgc tctttaaact atagattaat acttaagtta
     8581 aactcataca tactgatacc atacgttaga tctaacaatt taaaattcgt tataactatg
     8641 gattaaagag ctgcccaact catataatcc ttaaaaactt cacatgtgat tgtttattaa
     8701 gccctccttt atcttattaa atatccttat aaccctttta aaattaaact gacacactca
     8761 tacattgtta cacgcgacga taaacgtcat attattgcat atcattaaaa caagtcactt
     8821 tgtatcgtgt gcgaccgaat acatattaaa tcatcaataa tgattattca attattaaaa
     8881 ataaaaacga acattctata tagagtttct acttattatt ttttcagcat tgtctacatt
     8941 acttaacatt cgtgtttgta agtaatattg accgccaata tttagacact ttataagtat
     9001 gccattcatc atttttaatc atattatttt tcttattgat atttctattc
//
LOCUS       DAMQYT010000095         9019 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMQYT010000095 DAMQYT010000000
VERSION     DAMQYT010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.095"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            31..573
                     /locus_tag="PGA33_001689"
     CDS             31..573
                     /locus_tag="PGA33_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002493116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDG6473496.1"
                     /translation="MFRQFGYLPGFILLYIRAIIPVFPLALYILINIQAYGPILGILI
                     SWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLIPLFILLCFPFT
                     PNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTHPLRGILMLVVL
                     VVFWIVGKKLEQHFMGSKKE"
     gene            578..1102
                     /gene="lepB"
                     /locus_tag="PGA33_001690"
     CDS             578..1102
                     /gene="lepB"
                     /locus_tag="PGA33_001690"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDG6473497.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDQDNNKHDSRQFGLIDKKDIIGNVSL
                     RYYPFSKWTVQFKS"
     gene            1118..1693
                     /gene="lepB"
                     /locus_tag="PGA33_001691"
     CDS             1118..1693
                     /gene="lepB"
                     /locus_tag="PGA33_001691"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDG6473498.1"
                     /translation="MKKEILEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIVGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            1853..5329
                     /gene="addB"
                     /locus_tag="PGA33_001692"
     CDS             1853..5329
                     /gene="addB"
                     /locus_tag="PGA33_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDG6473499.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            5330..8983
                     /gene="addA"
                     /locus_tag="PGA33_001693"
     CDS             5330..8983
                     /gene="addA"
                     /locus_tag="PGA33_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDG6473500.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPKNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDSVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQIEDSSRPNVNISIVYFEDVSTETILDNDEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYRYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
BASE COUNT         3246 a         1259 c         1665 g         2849 t
ORIGIN      
        1 tcgtttcatc aagtagaaga atggtttgag atatttcgac agtttggtta tttacctgga
       61 tttatattgt tatatattag agctataatt ccagtatttc ctttagcact ctatatttta
      121 attaacattc aagcttatgg acctatttta ggtatattga ttagttggct tggattaatt
      181 tctggaacat ttacagtcta tttgatctgt aaacgattgg tgaacactga gaggatgcag
      241 cgaattaaac aacgtactgc tgttcaacgc ttgattagtt ttattgatcg ccaaggatta
      301 atcccattgt ttattttact ttgttttcct tttacgccaa atacattaat aaattttgta
      361 gcgagtctat ctcatattag acctaaatat tatttcattg ttttggcatc atcaaagtta
      421 gtttcaacaa ttattttagg ttatttaggt aaggaaatta ctacaatttt aacgcatcct
      481 ttaagaggga tattaatgtt agttgtgttg gttgtatttt ggattgttgg aaaaaagtta
      541 gaacagcatt ttatgggatc gaaaaaggag tgacatcgtg aaaaaagttg taaaatattt
      601 gatttcattg atacttgcta ttatcattgt actgttcgta caaacttttg taatagttgg
      661 tcatgtcatt ccgaataatg atatgtcacc aacccttaac aaaggggatc gtgttattgt
      721 aaataaaatt aaagttacat ttaatcaatt gaataatggt gatatcatta catataggcg
      781 tggtaacgag atatatacta gtcgaattat tgccaaacct ggtcaatcaa tggcgtttcg
      841 tcagggacaa ttataccgtg atgaccgacc ggttgacgca tcttatgcca agaacagaaa
      901 aattaaagat tttagtttgc gcaattttaa agaattagat ggtgatatta ttccgccaaa
      961 caattttgtt gtgctaaatg atcaagataa taacaagcac gattcaagac aatttggttt
     1021 aatcgataaa aaggatatta ttggtaatgt tagtttacga tactatcctt tttcaaaatg
     1081 gactgttcag ttcaaatctt aaaaagaggt gtcaaaattg aaaaaagaaa tattggaatg
     1141 gattatttca attgcagtcg cttttgtcat tttatttata gtaggtaaat ttattgttac
     1201 gccatataca attaaaggtg aatcaatgga tccaactttg aaagatggcg agcgagtagc
     1261 tgtaaacatt gttggatata aaacaggtgg tttggaaaaa ggtaatgtag ttgtcttcca
     1321 tgcaaacaaa aatgatgact atgttaaacg tgtcatcggt gttcctggtg ataaagtaga
     1381 atacaaaaat gatacattat atgtcaatgg taaaaaacaa gatgaaccat atttaaacta
     1441 caatttaaaa cataaacaag gtgattacat tactgggact ttccaagtta aagatttacc
     1501 gaatgcgaat cctaaatcaa atgtcattcc aaaaggtaaa tatttagtgc ttggagataa
     1561 tcgtgaagta agtaaagata gccgtgcgtt tggcctcatt gatgaagacc aaattgttgg
     1621 taaagtttca tttaggttct ggccatttag tgaatttaaa cataatttca atcctgaaaa
     1681 tactaaaaat taatatgaaa caaatacaac atcgtttgtc ggttttaata ctgataaacg
     1741 atgttttatt ttgttagtac cacaataaaa gctaagttcg aaatgaactt ataataaatc
     1801 aatcacaatc actttgtgtt aaaatatgtg tcaaaggaag tgagggtttg tcatgacatt
     1861 acatgcttat ttaggtagag cgggaacagg taagtctacg aaaatgttga ccgaaataaa
     1921 acaaaaaatg aaagcagatc cgcttggaga tccaatcatt ttaattgcgc caactcaaag
     1981 tacatttcaa ttagaacaag cctttgtcaa tgatccggaa ttaaatggta gtttaagaac
     2041 agaagtgttg cattttgaac gattaagtca tcgtattttc caagaagttg gtagttatag
     2101 cgaacaaaag ttatctaaag ctgcaacgga aatgatgatt tataacattg ttcaagaaca
     2161 acaaaagtat ttaaaacttt atcaatcaca agcaaaatat tatgggttta gtgaaaaatt
     2221 aacagaacaa attcaagatt ttaaaaaata tgcagtaacg cctgaacatt tagaacactt
     2281 tattgctgat aaaaatatgc aaactcgaac taaaaataag ttagaggata ttgctttaat
     2341 ataccgtgag ttcgaacaac gcattcaaaa cgagtttatt acgggtgagg attcattaca
     2401 atattttatt gattgtatgc cgaaatcaga gtggctaaaa cgtgctgata tatatattga
     2461 tggttttcac aacttttcaa cgattgagta tttaataatc aaaggattaa ttaaatatgc
     2521 gaagagtgtc acaattatat tgacgacaga tggtaaccac gatcaattta gtttatttag
     2581 aaaaccatcg gaagtgttac gacatattga agaaatagca aatgaactca atatttctat
     2641 tgaacgtcaa tatttcaacc aattatatcg cttcaataat caagatttaa agcatcttga
     2701 acaagaattt gatgcacttc aaatcaatcg agtggcatgt caaggtcata tcaatatttt
     2761 agaatctgcg actatgagag aggaaataaa tgaaattgcg cgacgtatca tcgttgatat
     2821 tcgtgataag caattacgat atcaagatat tgctatttta tatcgtgatg aatcttatgc
     2881 ttatttattt gattccatat taccgcttta taatattcct tataatattg atacaaagcg
     2941 ttcgatgaca catcatccgg tcatggaaat gattcgttca ttgattgaag ttattcaatc
     3001 taattggcaa gtgaatccaa tgctacgctt attgaagact gatgtgttaa cggcatcata
     3061 tctaaaaagt gcatacttag ttgatttact tgaaaatttt gtacttgaac gtggtatata
     3121 cggtaaacgt tggttagatg atgagctatt taatgtcgaa cattttagca aaatggggcg
     3181 taaagcgcat aaactgaccg aagatgaacg taacacattt gaacaagtcg ttaagttaaa
     3241 gaaagatgtc attgataaaa ttttacattt tgaaaagcaa atgtcacaag cggaaactgt
     3301 aaaagatttt gcaactgctt tttatgaaag tatggaatat ttcgaactgc caaatcaatt
     3361 gatgacagag cgagatgaac ttgatttaaa tggtaatcat gaaaaggcgg aggaaattga
     3421 tcaaatatgg aatggcttaa ttcaaatcct tgatgactta gttctagtat ttggagatga
     3481 accaatgtcg atggaacgtt tcttagaagt atttgatatt ggtttagaac aattagaatt
     3541 tgttatgatt ccgcaaacat tggaccaagt aagtattggt acgatggatt tggctaaagt
     3601 cgataataag caacatgttt acttagtagg tatgaatgat ggaacgatgc cacaaccagt
     3661 aactgcgtca agcttgatta cagatgaaga aaagaaatac tttgaacagc aggctaatgt
     3721 cgagttaagt ccaacatcag atattttaca gatggatgaa gcatttgttt gttatgttgc
     3781 tatgactaga gctaagggag atgttacatt ttcttacagt ctaatgggat caagtggtga
     3841 tgataaggag atcagcccat ttttaaatca aattcaatca ttgttcaacc aattggaaat
     3901 tactaacatt cctcaatacc atgaagttaa cccattgtca ctaatgcaac atgctaagca
     3961 aaccaaaatt acattatttg aagcattgcg tgcttggtta tatgatgaaa ttgtggctga
     4021 tagttggtta gatgcttatc aagtaattag agatagcgat catttaaatc aaggtttaga
     4081 ttatttaatg tcagcattaa cgtttgacaa tgaaactgta aaattaggtg aaacgttgtc
     4141 taaagattta tatggtaagg aaatcaatgc cagtgtatcc cgttttgaag gttatcaaca
     4201 atgcccattt aaacactatg cgtcacatgg tctgaaacta aatgagcgaa cgaagtatga
     4261 acttcaaaac tttgatttag gtgatatttt ccattctgtt ttaaaatata tatctgaacg
     4321 tattaatggc gattttaaac aattagacct gaaaaaaata agacaattaa cgaatgaagc
     4381 attggaagaa attttaccta aagttcagtt taatttatta aattcttcag cttactatcg
     4441 ttatttatca agacgcattg gcgctattgt agaaacaaca ctaagcgcat taaaatatca
     4501 aggcacgtat tcaaagttta tgccaaaaca ttttgagaca agttttagaa ggaaaccaag
     4561 aacaaatgac gaattaattg cacaaacatt aacgacaact caaggtattc caattaatat
     4621 tagagggcaa attgaccgta tcgatacgta tacaaagaat gatacaagtt ttgttaatat
     4681 cattgactat aaatcctctg aaggtagtgc gacacttgat ttaacgaaag tatattatgg
     4741 tatgcaaatg caaatgatga catacatgga tatcgtttta caaaataaac aacgccttgg
     4801 attaacagat attgtgaaac caggtggatt attatacttc catgtacatg aacctagaat
     4861 taaatttaaa tcatggtctg atattgatga agataaacta gaacaagatt taattaaaaa
     4921 gtttaagttg agtggtttag ttaatgcaga ccaaactgtt attgatgcat tggatattcg
     4981 tttagaacct aaattcactt cagatattgt accagttggt ttgaataaag atggctcttt
     5041 gagtaaacga ggcagccaag tggcagatga agcaacgatt tataaattca tccaacataa
     5101 caaagagaat tttatagaaa cagcttcaaa tattatggat ggacatactg aagttgcacc
     5161 attaaagtac aaacaaaaat tgccatgtgc tttttgtagt tatcaatcgg tatgtcatgt
     5221 agatggcatg attgatagta agcgatatcg aactgtagat gaaacaataa atccaattga
     5281 agcaattcaa aatattaaca ttaatgatga atttgggggt gagcaataga tgacaattcc
     5341 agagaaacca caaggcgtga tttggactga cgcgcaatgg caaagtattt acgcaactgg
     5401 acaagatgta cttgttgcag ccgcggcagg ttcaggtaaa acagctgtac tagttgagcg
     5461 tattatccaa aagattttac gtgatggcat tgatgtcgat cgacttttag tcgtaacgtt
     5521 tacaaactta agcgcacgtg aaatgaagca tcgtgtagac caacgtattc aagaggcatc
     5581 gattgctgat cctgcaaatg cacacttgaa aaaccaacgc atcaaaattc atcaagcaca
     5641 aatatctaca ctccatagtt tttgcttgaa attaattcaa cagcattatg atgtattaaa
     5701 tattgacccg aactttagaa caagcagtga agctgaaaat attttattat tagaacaaac
     5761 gatagatgag gtcatagaac aacattacga tatccttgat cctgctttta ttgaattaac
     5821 agagcaattg tcttcagata gaagtgatga tcagtttcga atgattatta aacaattgta
     5881 tttctttagc gttgcaaatc caaatcctaa aaattggttg gatcaattgg tgacaccata
     5941 cgaagaagaa gcacaacaag cgcaacttat tcaactacta acagacttat ctaaagtatt
     6001 tatcacagct gcttatgatg ctttaaataa ggcgtatgat ttgtttagta tgatggatag
     6061 cgtcgataaa catttagctg ttatagaaga tgaacgacgt ttaatggggc gtgttttaga
     6121 aggtggcttt attgatatac cttatttaac tggtcacgaa tttggcgcgc gtttgcctaa
     6181 tgtaacagcg aaaattaaag aagcaaatga aatgatggtc gatgccttag aagatgctaa
     6241 acttcagtat aaaaaatata aatcattaat tgataaagtg aagagtgatt acttttcaag
     6301 agaagctgat gatttgaaag ctgatatgca acaattggcg ccacgagtaa agtaccttgc
     6361 gcgtattgtg aaagatgtta tgtcagaatt caatcgaaaa aagcgtagca aaaatatttt
     6421 ggatttttct gattatgaac attttgcatt acaaatttta actaatgagg atggttcgcc
     6481 ttcagaaatt gccgaatcat accgtcaaca cttccaagaa atattggtcg atgagtatca
     6541 agatacgaac cgagttcaag agaaaatact atcttgcatc aaaacgggtg atgaacataa
     6601 tggtaattta tttatggttg gagatgttaa gcaatccatt tataaattta gacaagctga
     6661 tccaagttta tttattgaaa agtatcaacg ctttactata gatggagatg gcactggacg
     6721 tcgaattgat ttgtcgcaaa acttccgttc tcgaaaagaa gtactgtcaa cgactaacta
     6781 tatattcaaa catatgatgg atgaacaagt cggtgaagta aaatatgatg aagcggcaca
     6841 gttgtattat ggtgcaccat atgatgaatc ggaccatcca gtaaacttaa aagtccttgt
     6901 tgaagcggat caagaacata gtgatttaac tggtagtgaa caagaagcgc attttatagt
     6961 agaacaagtt aaagatatct tagaacatca aaaagtttat gatatgaaaa caggaagcta
     7021 tagaagtgcg acatacaagg atatcgttat tctagaacgc agctttggac aagctcgcaa
     7081 tttacaacaa gcttttaaaa atgaagatat tccattccat gtgaatagtc gtgaaggtta
     7141 ctttgaacaa acagaagtcc gcttagtatt atcattttta agagcgatag ataatccatt
     7201 acaagatatt tatttagttg ggttaatgcg ctccgttata tatcagttca aagaagacga
     7261 attagctcaa attagaatat tgagtccaaa tgatgactac ttctatcaat cgattgtaaa
     7321 ttacattaat gacgaagcag cagatgcaat tttagttgat aaattaaaaa tgtttttatc
     7381 agatattcaa agttaccaac aatatagtaa agatcatccg gtgtatcagt taattgataa
     7441 attttataat gatcattatg ttattcaata ctttagtgga cttattggtg gacgtggacg
     7501 acgtgcaaat ctttatggtt tatttaataa agctatcgag tttgagaatt caagttttag
     7561 aggtttatat caatttattc gttttatcga tgaattgatt gaaagaggca aagattttgg
     7621 tgaggaaaat gtagttggtc caaacgataa tgtcgttaga atgatgacaa ttcatagtag
     7681 taaaggtcta gagtttccat ttgtcattta ttctggattg tcaaaagatt ttaataaacg
     7741 tgatttgaaa caaccagtta ttttaaatca gcaatttggt ctcggaatgg attattttga
     7801 tgtggataaa gaaatggcat ttccatcttt agcttcggtt gcatatagag ctgttgccga
     7861 aaaagaactt gtgtcagaag aaatgcgatt agtctatgta gcattaacaa gagcgaaaga
     7921 acaactttat ttaattggta gagtgaaaaa tgataaatca ttactagaac tagagcaatt
     7981 gtctatttct ggtgagcaca ttgctgtcaa tgaacgatta acttcaccaa atccgttcca
     8041 tcttatttat agtattttat ctaaacatca atctgcgtca attccagatg atttaaaatt
     8101 tgaaaaagat atagcacaaa ttgaagatag tagtcgtccg aatgtaaata tttcaattgt
     8161 gtactttgaa gatgtgtcta cagaaaccat tttagataat gatgaatatc gttcggttaa
     8221 tcaattagaa actatgcaaa atggtaatga agatgttaaa gcacaaatta aacaccaact
     8281 tgattatcga tatccatatg taaatgatac taaaaagccc tcaaaacaat ctgtttctga
     8341 attgaaaaga caatatgaaa cagaagaaag tggcacaagt tacgaacgag taaggcaata
     8401 tcgtatcggt ttttcaacgt atgaacgacc taaatttcta agtgaacaag gtaaacgaaa
     8461 agcgaatgaa attggtacgt taatgcatac agtgatgcaa catttaccat tcaaaaaaga
     8521 acgcatatct gaagttgagt tacatcagta tatcgatgga ttaatcgata aacatattat
     8581 cgaagcagat gcgaaaaaag atatccgtat ggatgaaata atgacattta tcaatagtga
     8641 gttatattcg attattgctg aagcagagca agtttatcgt gaattaccgt ttgtagttaa
     8701 ccaagcatta gttgaccaat tgccacaagg agacgaagac gtctcaatta ttcaaggtat
     8761 gattgactta atctttgtta aagatggtgt gcattatttt gtagactata aaaccgatgc
     8821 atttaatcgt cgccgtggga tgacagatga agaaattggt acacaattaa aaaataaata
     8881 taagatacag atgaaatatt atcaaaatac gcttcaaacg attcttaata aagaagttaa
     8941 aggttattta tacttcttca aatttggtac attgcaacta tagtattttg attttcaaaa
     9001 gaataaaaaa taatttcga
//
LOCUS       DAMQYT010000096         9002 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMQYT010000096 DAMQYT010000000
VERSION     DAMQYT010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9002
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.096"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            4..411
                     /locus_tag="PGA33_001694"
     CDS             4..411
                     /locus_tag="PGA33_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473501.1"
                     /translation="MSSIIGKIAIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLL
                     GLILALFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFLSSILWLIGGILLIW
                     NKYSKDESTDENEKVDIESTENQFESKDKITKE"
     gene            461..1087
                     /locus_tag="PGA33_001695"
     CDS             461..1087
                     /locus_tag="PGA33_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDG6473502.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISKGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVAAADTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEVKNPTTKNLEGFNEEIHKDEATTKVVSDNTEPPIESKDHVSKKD"
     gene            1299..2075
                     /locus_tag="PGA33_001696"
     CDS             1299..2075
                     /locus_tag="PGA33_001696"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HDG6473503.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            2219..2341
                     /locus_tag="PGA33_001697"
     CDS             2219..2341
                     /locus_tag="PGA33_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473504.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNKNSQSYLILFL"
     gene            2411..3391
                     /locus_tag="PGA33_001698"
     CDS             2411..3391
                     /locus_tag="PGA33_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDG6473505.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNNHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            3354..4046
                     /locus_tag="PGA33_001699"
     CDS             3354..4046
                     /locus_tag="PGA33_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDG6473506.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDKLEEVRKSYYPI
                     KRAIDLILSIVLLFLTLPIMVIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            4256..5422
                     /locus_tag="PGA33_001700"
     CDS             4256..5422
                     /locus_tag="PGA33_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDG6473507.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHNDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRIVFTAHGWAFTEGVKPAKKFLYLVIEKLMSLITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENEVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            5403..6641
                     /locus_tag="PGA33_001701"
     CDS             5403..6641
                     /locus_tag="PGA33_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDG6473508.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMSVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            6631..8061
                     /locus_tag="PGA33_001702"
     CDS             6631..8061
                     /locus_tag="PGA33_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDG6473509.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVINLVLCIILIYFYGIYGAAIARLITEFF
                     LLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
     gene            8329..8928
                     /locus_tag="PGA33_001703"
     CDS             8329..8928
                     /locus_tag="PGA33_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDG6473510.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
BASE COUNT         3059 a         1257 c         1636 g         3050 t
ORIGIN      
        1 agtatgagct caattatagg aaaaatagca atttggatag gcatcgtagc tcaaatatat
       61 tttagtgtcg tttttgttag gatgatatct attaatattg ctggaggatc tgattacgaa
      121 acaatttttt tattaggatt aatattggct cttttcactg ttttaccaac catctttact
      181 gcgatttata tggaaagtta ctctgtaatc ggaggtgcac tttttattgt ttatgctatt
      241 attgcactgt gtttatataa tttcctttcg tcaattttat ggctgattgg tggtattttg
      301 ctgatttgga ataaatactc aaaagatgaa tcgacagacg aaaatgaaaa agttgatatt
      361 gaaagtacag agaatcaatt tgaatctaaa gataaaatca ctaaagaata aagagaatat
      421 ttaaggtaaa gtataaattt taaataaatg gggaatagac atggaaaaaa atgtagaaaa
      481 atcattcata aagataggtt tatattttca aatagcttat atagtactca tggctataac
      541 tttatgtggg tttgtaattt gctatggact aattttcggc cttttctatt tattatcagg
      601 tagcagagct gattatttaa tagtaacaat agttatatcg gcaataattt ctatatttgt
      661 aattatactt tcaatcgtac ctgtcatcgt attggcatct gacttattta aagaaaggat
      721 ttcaaaaggt gtcatattaa ttgtattggc tattatcgct ttagtattat gcaactttgt
      781 atctgcaata ctctggtttg tttcagccat atctatttta ggtagaaaaa aattagtagc
      841 tgcagcagat actaccacta ttcaaaaaag taaagggaac gcaaatcaag catcacataa
      901 agacacgtgt aaaaaggaac ttgatagtca agacatgatg gaacatcctg aggttaaaaa
      961 tcccacgact aaaaaccttg aaggatttaa cgaagaaata cataaagatg aagctacaac
     1021 taaagttgtc agtgataaca cggaaccgcc tattgaatca aaagaccatg tctcgaaaaa
     1081 agattgatga caaactaatc gagagactta aaaaaataat attcaacata agaactttta
     1141 aaacgacatt taaacgcatt gccaatcact aatggtagtg cgtttaacta taccttaaat
     1201 atctgaatat tttgttaaat ggagctacct ttgttgtact attcaaatga agaggagtaa
     1261 aatgtaatta aaggaaagaa atttgaggag tgatctttat gacaaacaac aaagtagcat
     1321 tagtaactgg cggagcacaa gggattggtt ttaaaattgc agaacgttta gtggaagatg
     1381 gtttcaaagt agcagttgtt gatttcaatg aagaaggggc aaaagcagct gcacttaaat
     1441 tatcaagtga tggtacaaaa gctattgcta tcaaagcaga tgtatcaaac cgtgatgatg
     1501 tatttaacgc agtaagacaa actgccgcgc aatttggcga tttccatgtc atggttaaca
     1561 atgccggcct tggaccaaca acaccaatcg atacaattac tgaagaacag tttaaaacag
     1621 tatatggcgt gaacgttgca ggtgtgctat ggggtattca agccgcacat gaacaattta
     1681 aaaaattcaa tcatggcggt aaaattatca atgcaacatc tcaagcaggc gttgagggta
     1741 acccaggctt gtctttatat tgcagtacaa aattcgcagt gcgaggttta acacaagtag
     1801 ccgcacaaga tttagcgtct gaaggtatta ctgtgaatgc attcgcacct ggtatcgttc
     1861 aaacaccaat gatggaaagt atcgcagtgg caacagccga agaagcaggt aaacctgaag
     1921 catggggttg ggaacaattt acaagtcaga ttgctttggg cagagtttct caaccagaag
     1981 atgtttcaaa tgtagtgagc ttcttagctg gtaaagactc tgattacatt actggacaaa
     2041 caattattgt agatggtggt atgagattcc gttaataatc atccactaat gataaataaa
     2101 tccttattgt taagtttaat cacttagcag taaggatttt ttagtgcact tagaagggag
     2161 tgtattggta gaaaattaat aagcgaagtt cttaagtgag ttatgatgtc acagtctaat
     2221 gcatcagttg aaagcattat tagtattaac acacccaaga tattataaaa catcacaaaa
     2281 acaccactat ctaatttatc tcaataaaaa ttcacaaagt tatctcattt tatttttata
     2341 aataaaaaat atcgataaaa agcttacaat actttatgtt tttatgatat atttttaatg
     2401 tataaatgag gtggaagatt tggaaagagt tttgataact ggtggggctg gttttattgg
     2461 gtcgcattta gtagatgatt tacaacaaga ttatgatgtt tatgttctag ataactatag
     2521 aacaggtaaa cgagaaaata ttaaaagttt ggctgacgat catgtgtttg aattagatat
     2581 tcgtgaatat gatgcagttg aacaaatcat gaagacatat caatttgatt atgttattca
     2641 tttagcagca ttagttagtg ttgctgagtc ggttgagaaa cctatcttat ctcaagaaat
     2701 aaacgtcgta gcaacattaa gattgttaga aatcattaaa aaatataata atcatataaa
     2761 acgttttatc tttgcttcgt cagcagctgt ttatggtgat cttcctgatt tgcctaaaag
     2821 tgatcaatca ttaatcttac cattatcacc atatgcaata gataaatatt acggcgaacg
     2881 gacgacatta aattattgtt cgttatataa cataccaaca gcggttgtta aattttttaa
     2941 tgtatttggg ccaagacagg atcctaagtc acaatattca ggtgtgattt caaagatgtt
     3001 cgattcattt gagcataaca agccatttac attttttggt gacggactgc aaactagaga
     3061 ttttgtatat gtatatgatg ttgttcaatc tgtacgctta attatggaac acaaagatgc
     3121 aattggacac ggttataaca ttggtacagg cacttttact aatttattag aggtttatcg
     3181 tattattggt gaattatatg gaaaatcagt cgagcatgaa tttaaagaag cacgaaaagg
     3241 agatattaag cattcttatg cagatatttc taacttaaag gcattaggat ttgttcctaa
     3301 atatacagta gaaacaggtt taaaggatta ctttaatttt gaggtagata atattgaaga
     3361 agttacagct aaagaagtgg aaatgtcgtg aaaatgacat tgaagctgtc cataataata
     3421 agggttatgc ctatcaaaga aaattagaca aactagaaga agtgagaaaa agctattacc
     3481 caattaaacg tgcgattgac ttaattttaa gcattgtttt attattttta actttaccga
     3541 ttatggttat attcgccatt gctatcgtca tagattcgcc aggaaaccct atttatagtc
     3601 aggttagagt tgggaagatg ggtaaattaa ttaaaatata caaattacgt tcgatgtgca
     3661 aaaacgcaga gaaaaacggt gcgcaatggg ctgataaaga tgatgatcgt ataacaaatg
     3721 tcgggaagtt tattcgtaaa acacgcattg atgaattacc acaactaatt aatgttgtta
     3781 aaggggaaat gagttttatt ggaccacgcc cggaacgtcc ggaatttgta gaattattta
     3841 gttcagaagt gataggtttc gagcaaagat gtcttgttac accagggtta acaggacttg
     3901 cgcaaattca aggtggatat gacttaacac cgcaacaaaa actgaaatat gacatgaaat
     3961 atatacataa aggtagttta atgatggaac tatatatatc aattagaaca ttgatggttg
     4021 ttattacagg ggaaggctca aggtagtctt aatttactta ataagttcaa ataaaagtta
     4081 tattttaaag attgtgacca attgttacag tataacgagg aatcccttga gacagtatca
     4141 aatggcatta agaaatatgt gccatcattg atttgcatgg ctataaatac tattcatctg
     4201 atgagatagc catgttaaga aattgaaagt atagcattaa aggggtttgt aacagttgaa
     4261 aattatatat tgtattacta aagcagacaa tggtggtgca caaacacatc tcattcaact
     4321 cgccaaccat ttttgcgtac acaatgatgt ttatgtcatt gtaggcaatc atggaccaat
     4381 gattgaacaa ctagatgcaa gagttaatgt aattattatc gaacatttag taggtccaat
     4441 tgactttaaa caagatattt tagctgtcaa agtgttagca cagttattct cgaaaattaa
     4501 acctgatgtt atccatttac attcttccaa agctggaacg gtcggacgaa ttgcgaagtt
     4561 catttcgaaa tcgaaagaca cacgtatagt ttttactgca catggatggg cttttacaga
     4621 gggtgttaaa ccagctaaaa aatttctata tttagttatc gaaaaattaa tgtcacttat
     4681 tacagatagc attatttgtg tttcagattt cgataaacag ttagcgttaa aatatcgatt
     4741 taatcgattg aaattaacca caatacataa tggtattgca gatgttcccg ctgttaagca
     4801 aacgctaaaa agccaatcac ataacaatat tggcgaagta gttggaatgt tgcctaataa
     4861 acaagattta cagattaatg ccccgacaaa gcatcaattt gttatgattg caagatttgc
     4921 ttatccaaaa ttgccacaaa atctaatcgc ggcaatagag atattgaaat tacataacag
     4981 taatcatgcg cattttacat ttataggcga tggacctaca ttaaatgatt gtcagcaaca
     5041 agttgtacaa gctgggttag aaaatgaagt cacatttttg ggcaatgtca ttaatgcgag
     5101 tcatttatta tcacaatacg atacgtttat tttaataagt aagcatgaag gtttgccaat
     5161 tagcattata gaagctatgg ctacaggttt gcctgttata gccagtcatg ttggcggtat
     5221 ttcagaatta gtagctgata atggtatatg tatgatgaac aaccaacccg aaactattgc
     5281 taaagtcctg gaaaaatatt taatagacag tgattacatc aaaatgagta atcaatctag
     5341 aaaacgttat ttagaatgtt ttactgagga gaaaatgatt aaagaagtgg aagacgttta
     5401 taatggaaaa tcaacacaat agtaaattac taacattgtt acttatcggt ttagcggttt
     5461 ttattcagca atcttcggtt attgccggtg tgaatgtttc tatagctgac tttatcacat
     5521 tactaatatt agtttattta ctgtttttcg ctaaccattt attaaaggca aatcattttt
     5581 tacagttttt cattattttg tatacatatc gtatgattat tacgctttgt ttgctatttt
     5641 ttgatgattt gatatttatt acggttaagg aagttcttgc atctacagtt aaatatgcat
     5701 ttgtagtcat ttatttctat ttagggatga tcatctttaa gttaggtaat agcaaaaaag
     5761 tgatcgttac ctcttatatt ataagcagtg tgactatagg tctattttgt attatagctg
     5821 gtttgaacaa gtccccttta ctaatgaaat tgttatattt tgatgaaata cgttcaaaag
     5881 gattaatgaa tgaccctaac tatttcgcga tgacacagat tattacattg gtacttgctt
     5941 acaagtatat tcataattac atattcaagg tccttgcatg tggtattttg ctatggtctt
     6001 taactacaac ggggtctaag actgcgttta tcatattaat cgtcttagcc atttatttct
     6061 ttattaaaaa gttatttagt agaaatgcgg taagtgttgt gagtatgtca gtgattatgc
     6121 tgatattact ttgttttacc ttttataata tcaactacta tttattccaa ttaagcgacc
     6181 ttgatgcctt accgtcatta gatcgaatgg cgtctatttt tgaagagggc tttgcatcat
     6241 taaatgatag tgggtctgag cgaagtgttg tatggataaa tgccatttca gtaattaaat
     6301 atacactagg ttttggtgtc ggattagtgg attatgtaca tattggctcg caaattaatg
     6361 gtattttact tgttgcccat aatacatatt tgcagatctt tgcggaatgg ggcattttat
     6421 tcggtgcatt atttatcata tttatgcttt atttactgtt tgaattattt agatttaaca
     6481 tttctgggaa aaatgtaaca gcaattgttg taatgttgac gatgctgatt tactttttaa
     6541 cagtatcatt taataactca agatatgtcg cttttatttt aggaattatc gtctttattg
     6601 ttcaatatga aaagatggaa agggatcgta atgaagagtg attcactaaa agaaaatatt
     6661 atttatcaag ggctatacca attgattaga acgatgacac cactgattac aatacccatt
     6721 atttcacgtg catttggtcc cagtggtgtg ggtattgttt cattttcttt caatatcgtg
     6781 caatactttt tgatgattgc aagtgttggc gttcagttat attttaatag agttatcgcg
     6841 aagtccgtta acgacaaacg gcaattgtca cagcagtttt gggatatctt tgtcagtaaa
     6901 ttatttttag cgttaacagt ttttgcgatg tatatggtcg taattactat atttattgat
     6961 gattactatc ttattttcct actacaagga atctatatta taggtgcagc actcgatatt
     7021 tcatggtttt atgctggaac tgaaaagttt aaaattccta gcctcagtaa tattgttgcg
     7081 tctggtattg tattaagtgt agttgttatt tttgtcaaag atcaatcaga tttatcattg
     7141 tatgtattta ctattgctat tgtgacggta ttaaaccaat tacctttgtt tatctattta
     7201 aaacgataca ttagctttgt ttcggttaat tggatacacg tctggcaatt gtttcgttcg
     7261 tcattagcat acttattacc aaatggacag ctcaacttat atactagtat ttcttgcgtt
     7321 gttcttggtt tagtaggtac ataccaacaa gttggtatct tttctaacgc atttaatatt
     7381 ttaacggtcg caatcataat gattaataca tttgatcttg taatgattcc gcgtattacc
     7441 aaaatgtcta tccagcaatc acatagttta actaaaacgt tagctaataa tatgaatatt
     7501 caattgatat taacaatacc tatggtcttt ggtttaattg caattatgcc atcattttat
     7561 ttatggttct ttggtgagga attcgcatca actgtcccat tgatgaccat tttagcgata
     7621 cttgtattaa tcattccttt aaatatgttg ataagcaggc aatatttatt aatagtgaat
     7681 aaaataagat tatataatgc gtcaattact attggtgcag tgataaacct agtattatgt
     7741 attattttga tatattttta tggaatttac ggtgctgcta ttgcgcgttt aattacagag
     7801 tttttcttgc tcatttggcg atttattgat attactaaaa tcaatgtgaa gttgaatatt
     7861 gtaagtacga ttcaatgtgt cattgctgct gttatgatgt ttattgtgct tggtgtggtc
     7921 aatcattatt tgccccctac aatgtacgct acgctgctat taattgcgat tggtatagta
     7981 gtttatcttt tattaatgat gactatgaaa aatcaatacg tatggcaaat attgaggcat
     8041 cttcgacata aaacaattta agtaccggta atgctatact ttagaaaatt aagattaaga
     8101 agaaaaggca atttcttatt gaaaaatgga agttgtcttt tttaattctc tttaaaagcg
     8161 ggaaacaaaa gcagttaaat gcctttttgc attcaatatt aaatattata tcaatttcga
     8221 atatttaaat tttatataat tggatataac aaataaataa taattattgc aaaacacacc
     8281 cgaaattaat tattataaaa gtatattcat aaaaggagga atatacttat ggcatttaaa
     8341 ttaccaaatt taccatatgc atatgatgca ttggaaccat atatagatca aagaacaatg
     8401 gagtttcatc acgacaaaca tcacaatacg tacgtgacga aattaaacgc aacagttgaa
     8461 ggaacagagt tagagcatca atcactagcg gatatgattg ctaacttaga caaggtaccg
     8521 gaagcgatga ggatgtcagt ccgtaataat ggcggtggtc attttaacca ttcattattc
     8581 tgggaaatac tatcacctaa ttctgaagaa aaaggtggcg taatagatga catcaaagcg
     8641 cagtggggca ctttagatga atttaaaaat gaatttgcaa ataaagcaac aacattattt
     8701 ggatcaggtt ggacttggtt agttgttaat gatggcaaat tagaaattgt gacaacgcca
     8761 aaccaagata atccattaac agaaggcaaa acaccaatct tactatttga tgtttgggag
     8821 catgcctact atctgaaata tcaaaataaa cgtccagact atatgactgc attttggaac
     8881 attgttaact ggaaaaaagt tgatgaatta taccaagcag caaaataata taacttaata
     8941 taattgaggt ggagcatcta caaggtgttc cacttttttg tacttaatat ctttacttat
     9001 tt
//
LOCUS       DAMQYT010000097         8983 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMQYT010000097 DAMQYT010000000
VERSION     DAMQYT010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.097"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            4..975
                     /gene="ribF"
                     /locus_tag="PGA33_001704"
     CDS             4..975
                     /gene="ribF"
                     /locus_tag="PGA33_001704"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDG6473511.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            1090..1359
                     /gene="rpsO"
                     /locus_tag="PGA33_001705"
     CDS             1090..1359
                     /gene="rpsO"
                     /locus_tag="PGA33_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDG6473512.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
     gene            1729..3825
                     /gene="pnp"
                     /locus_tag="PGA33_001706"
     CDS             1729..3825
                     /gene="pnp"
                     /locus_tag="PGA33_001706"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDG6473513.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            4061..5734
                     /locus_tag="PGA33_001707"
     CDS             4061..5734
                     /locus_tag="PGA33_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDG6473514.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            5990..8359
                     /locus_tag="PGA33_001708"
     CDS             5990..8359
                     /locus_tag="PGA33_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDG6473515.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHREVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            8364..>8983
                     /locus_tag="PGA33_001709"
     CDS             8364..>8983
                     /locus_tag="PGA33_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDG6473516.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLK"
BASE COUNT         3246 a         1254 c         1767 g         2716 t
ORIGIN      
        1 tttatgaaag tcatagaagt gacacatcct atacaatcta aacagtatat tacagaggat
       61 gttgcaatgg cattcggatt tttcgatggc atgcataaag gtcatgacaa agtctttgat
      121 atattaaacg aaatagctga ggcacgcagt ttaaaaaaag cggtgatgac atttgatccg
      181 catccgtctg tcgtgttgaa tcctaaaaga aaacgaacaa cgtatttaac gccactttca
      241 gataaaatcg aaaaaattag ccaacatgat attgattatt gtatagtggt taatttttca
      301 tctaggtttg ctaatgtgag cgtagaagat tttgttgaaa attatataat taaaaataat
      361 gtaaaagaag tcattgctgg ttttgatttt acttttggta aatttggaaa aggtaatatg
      421 actgtacttc aagaatatga tgcgtttaat acgacaattg tgagtaaaca agaaattgaa
      481 aatgaaaaaa tttctacaac ttctattcgt caagatttaa tcaatggtga gttgcaaaaa
      541 gcgaatgatg ctttaggcta tatatattct attaaaggca ctgtagtgca aggtgaaaaa
      601 aggggaagaa ctattggctt cccaacagct aacattcaac ctagtgatga ttatttgtta
      661 cctcgtaaag gtgtttatgc tgttagtatt gaaatcggca ctgaaaataa attatatcga
      721 ggggtagcta acataggtgt aaagccaaca tttcatgatc ctaacaaagc agaagttgtc
      781 atcgaagtga atatctttga ctttgaggat aatatttatg gtgaacgagt gaccgtgaat
      841 tggcatcatt tcttacgtcc tgagattaaa tttgatggta tcgacccatt agttaaacaa
      901 atgaacgatg ataaatcgcg tgctaaatat ttattagcag ttgattttgg tgatgaagta
      961 gcttataata tctagagttg cgtatagtta tataaacaat ctataccaca cctttttctt
     1021 agtaggtcga atctccaacg cctaactcgg attaaggagt attcaaacat tttaaggagg
     1081 aaattgatta tggcaatttc acaagaacgt aaaaacgaaa tcattaaaga ataccgtgta
     1141 cacgaaactg atactggttc accagaagta caaatcgctg tacttactgc agaaatcaac
     1201 gcagtaaacg aacacttacg tacacacaaa aaagaccacc attcacgtcg tggattatta
     1261 aaaatggtag gtcgtcgtag acatttatta aactacttac gtagtaaaga tattcaacgt
     1321 taccgtgaat taattaaatc acttggtatc cgtcgttaat cttaatataa cgtctttgag
     1381 gttggggcat atttatgttc caaccttaat ttatattaaa aaagcttttt acaaatatta
     1441 acatttatta tatgttaagc taatattgag tgaataataa ggttacaatg agataaagat
     1501 gatataagta cacctagagt aataatcaag atattaaaaa taaagtatgt ttttttaaaa
     1561 aatataactt atatttatac tgataagggt gggacgataa gtctattttg taaataatag
     1621 atggatatcc cgctctcttt ttttccaatt caatatttta taactaatat taaaatacga
     1681 taataaatga tatgatataa ctattagatt caagagagga gatttataat gtctcaagaa
     1741 aagaaagttt ttaaaactga atgggcagga agatctttaa cgattgaaac agggcaatta
     1801 gctaaacaag caaatggcgc tgtattggtt cgttatggag atacagtcgt gttatcgacg
     1861 gcaactgcat caaaagaacc tcgtgatgga gatttcttcc cattaacagt gaactatgaa
     1921 gaaaaaatgt acgctgcggg taaaattcct ggtggattta aaaagagaga aggacgtcct
     1981 ggtgacgatg caacattaac tgcgcgatta attgatagac caattagacc tttattccct
     2041 aaaggatata agcatgatgt tcaaattatg aacatggtat taagtgcaga tcctgattgt
     2101 tcaccacaaa tggctgcaat gattggttca tctatggcgc ttagtgtgtc ggatattcca
     2161 ttccaagggc caatcgccgg tgtaaatgtg ggttatattg acggtaaata tatcattaac
     2221 ccaacagtag aagaaaaaga agtttctcgt ttagaccttg aagtagctgg tcataaagat
     2281 gcggtaaaca tggtagaggc aggcgctagt gagattactg aacaagaaat gttagaggcg
     2341 attttctttg gtcatgaaga gattcaacgt ttagttgatt tccaacaaca aatcgtcgac
     2401 cacattcaac ctgttaaaca agaatttatt ccagcagagc gtgatgaagc gctagttgaa
     2461 cgtgtaaaat ctttaaccga agaaaaagga cttaaagaaa cagttttaac atttgataaa
     2521 caacaacgag atgaaaatct tgataactta aaagaagaaa tcgtcaatga atttatcgat
     2581 gaagaagatc cagagaatga attacttatt aaagaagttt atgcaatttt aaatgaatta
     2641 gtgaaagaag aagttcgacg tttaattgca gatgaaaaaa ttagaccaga cggccgtaaa
     2701 cctgatgaaa tccgtccatt agattctgaa gttggtattt tacctagaac gcatggttca
     2761 ggtctattta cacgtggtca gactcaagca ctttcagttt taacattagg tgctttaggc
     2821 gattatcaat taattgatgg tttaggacct gaagaagaaa aaagattcat gcatcattac
     2881 aacttcccga atttttcagt aggtgaaact ggtccagtac gtgcgccagg tcgtcgtgaa
     2941 attggacatg gtgcgttagg tgaaagagca ttaaaatata ttattcctga tactgctgat
     3001 ttcccatata caattcgtat tgtaagtgag gtacttgaat caaatggttc atcatctcaa
     3061 gcgtcaattt gtggatcaac attagcatta atggatgcgg gcgtaccgat taaagcacca
     3121 gttgctggta ttgctatggg ccttgttaca cgtgaagata gctatacgat tttaactgat
     3181 atccaaggta tggaagatgc attaggtgat atggacttta aagtcgctgg tactaaagaa
     3241 ggtattacag caatccaaat ggatattaaa attgacggtt taacgcgtga aattatcgaa
     3301 gaggctctag aacaagcgag acgtggtcgt ttagaaataa tgaatcatat gttacaaaca
     3361 attgatcaac cacgtactga attaagtgct tacgcgccaa aagttgtaac tatgacaatt
     3421 aaaccagata agattagaga tgttatcgga cctggtggta aaaaaattaa cgaaattatt
     3481 gatgaaacag gtgttaaatt agatattgaa caagatggta ctatctttat tggtgctgtt
     3541 gatcaagcta tgataaatcg tgctcgtgaa atcattgagg aaattacacg tgaagcggaa
     3601 gtaggtcaaa cttatcaagc cactgttaaa cgtattgaaa aatacggtgc gtttgtaggc
     3661 ctattcccag gtaaagatgc gttgcttcac atttcacaaa tttcaaaaaa tagaattgaa
     3721 aaagtggaag atgtattaaa aatcggtgac acaattgaag ttaagattac tgaaattgat
     3781 aaacaaggtc gagtaaatgc ttcacataga gcattagaag aataatattt aaagtcatat
     3841 gacgacaatg tatcgtcatg tgattttttt atgccacttt ttacgaagtg acccgttttg
     3901 aatttgttgt attgaacatt ttaaaacgct ttattatttt gtgtgcaact gttaattatc
     3961 ctgtatgtat agtgattaat agtgtacatc aagtgttttt taacttataa tgaatagtga
     4021 gtttatatat ggacgggtaa caaatttagg aggtaagatt ttgagtttaa taaagaaaaa
     4081 gaataaagat attcgcatta taccattagg cggtgttggc gaaattgcta aaaatatgta
     4141 tatcgttgaa gtagacgatg aaatgtttat gttagatgct ggacttatgt ttccagaaga
     4201 cgaaatgcta ggtattgata ttgttatacc agacatttca tacgtacttg aaaataaaga
     4261 taaattgaag ggtatattcc ttacacacgg acatgagcac gcgattggtg cagtgagtta
     4321 tgttttagaa caattagatg caccagtata tggatctaaa ttgacaatag cgttaattaa
     4381 agaaaatatg aaagcccgta atattgataa aaaagttcgc tactatacag ttaataatga
     4441 ttcaattatg agattcaaaa acgtgaatat tagtttcttt aatacgacac acagtattcc
     4501 tgatagttta ggtgtttgta ttcacacttc atatggtgcc attgtgtata caggtgaatt
     4561 taagtttgac caaagtttac atggacatta tgcaccagat attaaacgta tggcagagat
     4621 tggtgaagaa ggcgtatttg tcttaatcag tgattctact gaggcagaga aacctggata
     4681 taatactccg gaaaatgtga ttgaacatca tatgtatgat gcttttgcaa aagtgcgagg
     4741 tcgcttgata gtttcatgtt atgcttcgaa ctttatacgt attcagcaag ttttaaatat
     4801 tgctagcaag ctaaatcgta aagtgtcatt tttaggaaga tcacttgaaa gttcatttaa
     4861 tattgctcgt aaaatggggt atttcgacat tcctaaagat ttgctaattc ctataacaga
     4921 agttgataat tatcctaaaa atgaagtgat aattatagct actggtatgc aaggagaacc
     4981 tgtagaagcc ttaagtcaaa tggcgcaaca taagcataaa attatgaata tcgaagaagg
     5041 cgattctgta tttttagcaa ttacggcttc tgctaatatg gaagttatca ttgcgaatac
     5101 attaaatgag cttgtacgtg ctggcgcaca tattattcca aataacaaaa agattcatgc
     5161 ttcaagtcat ggttgcatgg aagaattaaa aatgatgatt aatattatga aacctgaata
     5221 ctttattcct gtacaaggtg aatttaaaat gcagatagca catgcgaagc tagcagctga
     5281 agcaggtgtt gcaccagaaa agattttcct tgtggaaaaa ggagatgtca ttaattacaa
     5341 cggtaaagat atgatattaa atgaaaaggt aaattcagga aatattttaa tagatggcat
     5401 tggtattggg gatgtaggaa atatcgtgtt gagagaccgt catcttttag cagaagatgg
     5461 tatctttatt gctgttgtaa cgttagatcc taaaaataga cgtatagctg cgggacctga
     5521 aattcaatct cgtgggtttg tatatgtacg tgaaagtgaa gacttattac gtgaagcaga
     5581 agagaaagta cgtgaaatag tagaggctgg tttacaagaa aaacgcatag aatggtctga
     5641 aattaaacaa aatatgcgtg atcaaattag taaactatta ttcgaaagta caaaacgtcg
     5701 tcctatgatt attccagtaa tttctgaaat ttaatcaaaa agtcattaac ataaaagagg
     5761 tcagaacaag tcactgaaat ataatggttg tcatggacaa tttacttata ttttatgatg
     5821 gtcaattgaa ggggtaacga ttaatctgtt atcttaagta aattgataca tagatgatat
     5881 tgttctaacc tctttcatcg tctgtttgga ctacatattc taaacatcaa ataggaaatt
     5941 atatataata acgtcgtttt aactaaggca acataaggag gtgcgtcaat tggcacaagc
     6001 aaaaaagaaa tcgacagcta agaaaaaaac aacatcaaaa aaaagaacaa attcgaggaa
     6061 aaagaagaat gataatccga tacgttatgt catagctatt ttagtagttg tattaatggt
     6121 gttgggtgtt ttccaattag gaataatagg tcgtctaatt gacagcttct ttaattattt
     6181 atttgggtac agtagatatt taacatatat tttagtactc ttagcaactg gttttattac
     6241 atactctaaa cgtattccta aaactagacg aacggctggt tcgattgtat tgcaaattgc
     6301 attgctattt gtatcacagt tagtttttca ttttaatagt ggtatcaaag ctgaaagaga
     6361 acctgtactt tcttatgtgt atcagtcata ccaacacagt catttcccaa attttggtgg
     6421 cggtgtatta ggcttttatt tattagagtt aagcgtacct ttaatttcat tatttggtgt
     6481 atgtattatt actattttat tattatgctc aagtgttatt ttattaacaa accatcaaca
     6541 tcgtgaagtt gcaaaagttg cactggaaaa tataaaagct tggtttggtt catttaatga
     6601 aaaaatgtcg gaaagaaacc aagaaaaaca attgaagcgt gaagaaaaag caagacttaa
     6661 agaagaacaa aaggcacgtc aaaatgaaca gccacaaata aaagatgtga gtgattttac
     6721 ggaagtgcct caagaaagag atattccaat ttatgggcat actgaaaatg aaagtaaaag
     6781 ccagagtcaa ccaagtcgaa aaaaacgagt gtttgatgca gagaatagtt cgaataacat
     6841 cgtaaatcat catcaagcag atcagcaaga acaattaaca gaacaaactc ataacagtgt
     6901 tgaaagtgaa aacactattg aagaagctgg tgaagttacg aatgtatcgt atgttgttcc
     6961 accgttaact ttacttaatc aacctgcaaa acaaaaagca acatctaaag ctgaagtgca
     7021 acgtaaagga caagtactag agaatacatt aaaagatttt ggggtaaatg caaaagtgac
     7081 acaaattaaa attggtcctg cagtaactca atatgaaatt caaccagctc aaggggttaa
     7141 agtgagtaaa attgtaaact tgcataatga tattgcatta gctttagcag caaaagatgt
     7201 tagaatcgaa gcgccaatac ctggtcgttc tgcagtaggt attgaagtgc caaatgagaa
     7261 aatttcatta gtttcactaa aagaagtttt agatgaaaaa ttcccgtcta ataataaact
     7321 agaagttgga ttaggaagag atatatcagg tgatccaatt actgttccac taaatgaaat
     7381 gccacactta ttggtggcag gatcgacggg tagtggtaaa tctgtttgta taaatggtat
     7441 tattacaagt attttattaa atgctaagcc gcatgaagtt aaacttatgt taatcgatcc
     7501 gaaaatggtt gaactaaatg tttataacgg aattccacac ttattaattc cggttgttac
     7561 aaatcctcat aaagctgctc aagctttaga aaaaattgta gctgagatgg aaagacgtta
     7621 tgatttattc caacattcat caactagaaa cattaaaggt tataacgaat taatccgtaa
     7681 gcaaaatcaa gaattagatg agaagcaacc agaattacct tatatcgttg ttattgtaga
     7741 tgagcttgca gatttaatga tggtagctgg taaagaagtt gaaaatgcga ttcaacgtat
     7801 tacacaaatg gcacgtgcag caggtataca tttaattgta gcgacacaaa gaccttctgt
     7861 ggatgtaatt acaggtatca ttaaaaataa tattccatct agaatagctt ttgctgtgag
     7921 ttctcaaaca gattcaagaa ctattattgg tactggcggc gcagaaaagt tacttggtaa
     7981 aggtgacatg ttatacgttg gaaatggtga ctcatcacaa acacgtattc aaggggcgtt
     8041 tttaagtgac caagaggtgc aagatgttgt aaattatgta gtagaacaac aacaggcaaa
     8101 ttatgtaaaa gaaatggaac cagatgcacc agtggataaa tcggaaatga aaagtgaaga
     8161 tgctttatat gatgaagcgt atttgtttgt tgttgaacaa caaaaggcaa gtacatcatt
     8221 gttacaacgc caatttagaa ttggttataa tagagcatct aggttgatgg atgatttaga
     8281 acgcaatcag gtaatcggtc cacaaaaagg aagcaagcct agacaagttt taatagatct
     8341 taataatgac gaggtgtaaa aaaatgtcag aaatgaatgc ggtatataac gttaaacaat
     8401 acattttaaa tttgattaag caaaataaat tggaatatgg tgaccaactt ccaagtaatt
     8461 tatcaattgc cagagaatta aatgtaaaaa ccgacgatgt ttatgaagca attcaggcat
     8521 tgattactga acaagtcatt aaagataatt ttgaagaggg cacaagtgtt aagtcactgc
     8581 cccctttctt ttatccattg aatgaactta taagtattgg gcaaatgatt aaaaatgcag
     8641 gatttgaatg cggaactgaa tacttaaatt ttgatgagca accagcaact atgttagatg
     8701 caaatttgtt gagcgttgaa gaaggatatc cagtaaccat tatagaacga ttacgaactg
     8761 ccgatggaga accggtcgtc tattgtttag ataaaattgc taaaaaagaa ttaacatgta
     8821 cagagtatca aatgagcaat ggatcgattc taagtgcgat aaaagaacaa agtaatcata
     8881 atatttgtta tgctgataca gaaattgaag cggtaaatta tgaacctcga atatccgaag
     8941 tactgaatgc ttcgccacac gaaggtttga ttttattaaa aat
//
LOCUS       DAMQYT010000098         8964 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMQYT010000098 DAMQYT010000000
VERSION     DAMQYT010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.098"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..3441
                     /locus_tag="PGA33_001710"
     CDS             <1..3441
                     /locus_tag="PGA33_001710"
                     /inference="COORDINATES: protein motif:HMM:NF028519.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HDG6473517.1"
                     /translation="AITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFGLGNQEAKA
                     AENTSTENAKQDDATTSDNKEVVSETENNSTTENNSTNPIKKETNTDSQPEAKKESTS
                     SSTQKQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNNTNNDVTTK
                     PSTSEPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKEELK
                     NNPEKLKELVRNDSNTDHSTKPVATAPTSVAPKRVNAKMRFAVAQPAAVASNNVNDLI
                     KVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKNVIPSDLTD
                     KNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKSRLTLYSYIDKKTVPN
                     ETSLNLTFATAGKETSQNVTVDYQDPMVHGDSNIQSIFTKLDEDKQTIEQQIYVNPLK
                     KSATNTKVDIAGSQVDDYGNIKLGNGSAIIDQNTEIKVYKVNSDQQLPQSNRIYDFSQ
                     YEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQGTSMRTTD
                     KYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTDSKEKP
                     MANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPTGYLPTKVNGTTDG
                     EKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANEPGIKDVK
                     VTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTTADDKDSNG
                     LTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVTVTLQNE
                     KGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDADGGEVDVT
                     ITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAG
                     KHTPVKPMSTTKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLFGRRKKQNK
                     "
     gene            3560..5032
                     /locus_tag="PGA33_001711"
     CDS             3560..5032
                     /locus_tag="PGA33_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDG6473518.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFVMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGAGNGIKSFYKTVFNNLTRYKAIVV
                     STEKQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPQGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(5156..6646)
                     /locus_tag="PGA33_001712"
     CDS             complement(5156..6646)
                     /locus_tag="PGA33_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDG6473519.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRY
                     LYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRI
                     YDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLC
                     EFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGD
                     LFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYR
                     AIIVSTEQQKLDVEKRINHTIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEK
                     QLHQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQE
                     YSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITSDFNGYLITKNDED
                     ALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(6928..7806)
                     /locus_tag="PGA33_001713"
     CDS             complement(6928..7806)
                     /locus_tag="PGA33_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HDG6473520.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
     gene            complement(7819..8484)
                     /gene="bshB2"
                     /locus_tag="PGA33_001714"
     CDS             complement(7819..8484)
                     /gene="bshB2"
                     /locus_tag="PGA33_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HDG6473521.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLANYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            complement(8498..8860)
                     /locus_tag="PGA33_001715"
     CDS             complement(8498..8860)
                     /locus_tag="PGA33_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HDG6473522.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQITYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
BASE COUNT         3289 a         1466 c         1485 g         2724 t
ORIGIN      
        1 gcaataacaa aaaagggtat gatttcaaat cgcttaaaca aattttcgat tagaaagtat
       61 actgtaggaa ctgcatcgat tttagtaggt acgacattga tttttggtct agggaaccaa
      121 gaagctaaag ctgctgaaaa cactagtaca gaaaatgcaa aacaagatga tgcaacgact
      181 agtgataata aagaagtagt gtcggaaact gaaaataatt cgacaacaga aaataattca
      241 acaaatccaa ttaagaaaga aacaaatact gattcacaac cagaagctaa aaaagaatca
      301 acttcatcaa gtactcaaaa acagcaaaat aacgttacag ctacaactga aactaagcct
      361 caaaacattg aaaaagaaaa tgttaaacct tcaactgata aaactgcgac agaagataca
      421 tctgttattt tagaagagaa gaaagcacca aataatacaa ataacgatgt aactacaaaa
      481 ccatctacaa gtgaaccatc tacaagtgaa attcaaacaa aaccaactac acctcaagaa
      541 tctacaaata ttgaaaattc acaaccgcaa ccaacgcctt caaaagtaga caatcaagtt
      601 acagatgcaa ctaatccaaa agaaccagta aatgtgtcaa aagaagaact taaaaataat
      661 cctgagaaat taaaagaatt ggttagaaat gatagcaata cagatcattc aactaaacca
      721 gttgctacag ctccaacaag tgttgcacca aaacgtgtaa acgcaaaaat gcgctttgca
      781 gttgcacaac cagcagcagt tgcttcaaac aatgtaaatg atttaattaa agtgacgaag
      841 caaacaatca aagttggcga tggtaaagat aatgtggcag cagcgcatga cggtaaagat
      901 attgaatatg atacagagtt tacaattgac aataaagtca aaaaaggcga tacaatgacg
      961 attaattatg ataagaatgt aattccttcg gatttaacag ataaaaatga tcctatcgat
     1021 attactgatc catcaggaga ggtcattgct aaaggaacat ttgataaagc aactaagcaa
     1081 atcacatata catttacaga ctatgtagat aaatatgaag atataaaatc acgcttaact
     1141 ctatattcgt atattgataa aaaaacagtt ccaaatgaga caagtttgaa tttaacattt
     1201 gctacagcag gtaaagaaac aagccaaaat gtcactgttg attatcaaga tccaatggtc
     1261 catggtgatt caaacattca atctatcttt acaaaattag atgaagataa gcaaactatt
     1321 gaacaacaaa tttatgttaa cccattgaaa aaatcagcaa ccaacactaa agttgatata
     1381 gctggtagtc aagtagatga ttatggaaat attaaactag gaaatggtag cgccattatt
     1441 gaccaaaata cagaaataaa ggtttataaa gttaactctg atcaacaatt gcctcaaagt
     1501 aatagaatct atgattttag tcaatacgaa gatgtaacaa gtcaatttga taataaaaaa
     1561 tcatttagta ataatgtagc aacattggat tttggtgata ttaattcagc ctatattatc
     1621 aaagttgtta gtaaatatac acctacatca gatggcgaac tagatattgc ccaaggtact
     1681 agtatgagaa caactgataa atatggttat tataattatg caggatattc aaacttcatc
     1741 gtaacttcta atgacactgg cggtggcgac ggtactgtta aacctgaaga aaagttatac
     1801 aaaattggtg actatgtatg ggaagacgtt gataaagacg gtgttcaagg tacagattca
     1861 aaagaaaaac caatggcaaa cgttttagtt acattaactt acccggacgg tactacaaaa
     1921 tcagtaagaa cagatgctaa tggtcattat gaattcggtg gtttgaaaga cggagaaact
     1981 tatacagtta aattcgaaac gccaactgga tatcttccaa caaaagtaaa tggaacaact
     2041 gatggtgaaa aagactcaaa tggtagttcg gttactgtta aaattaatgg taaagatgat
     2101 atgtctttag atactggttt ttacaaagaa cctaaataca acttaggtga ctatgtatgg
     2161 gaagatacta ataaagatgg tatccaagat gcaaatgagc caggaatcaa agatgttaag
     2221 gttacattaa aagatagtac tggaaaagtt attggtacaa ctactactga tgcctcgggt
     2281 aaatataaat ttacagattt agataatggt aactatacag tagaatttga aacaccagca
     2341 ggttacacgc caacggttaa aaatactaca gctgatgata aagattctaa tggtttaaca
     2401 acaacaggtg tcattaaaga tgcagataat atgacattag acagtggttt ctataaaaca
     2461 ccaaaataca gtttaggtga ttatgtttgg tacgacagta ataaagacgg caaacaagat
     2521 tcaactgaaa aaggtatcaa agatgtgaca gttacattgc aaaacgaaaa aggcgaagta
     2581 attggaacaa ctaaaacaga tgaaaatggt aaatatcgtt tcgataattt agatagcggt
     2641 aaatacaaag ttatttttga aaagcctgct ggcttaacac aaacagttac aaatacaact
     2701 gaagatgata aagatgcaga tggtggcgaa gttgacgtaa caattacgga tcatgatgat
     2761 ttcacacttg ataacggata cttcgaagaa gatacatcag acagcgattc agactcagat
     2821 agtgactcag acagcgactc agactcagac agcgactcag actcagacag tgattcagat
     2881 tcagacagcg actcagattc agatagcgac tcagattcgg acagcgattc agactcagat
     2941 agcgactcag attcagatag cgattcagac tcagacagcg actcagattc agatagcgat
     3001 tcggactcag acagcgattc agactcagat agcgactcag actcagacag cgactcagat
     3061 tcagatagcg attcggactc agatagcgac tcagattcag acagcgattc agactcagat
     3121 agcgactcag actcagacag tgattcagat tcagacagcg actcagactc agatagcgac
     3181 tcagattcgg acagcgactc agactctgat agcgactcag actcagacag tgattcagac
     3241 agcgattcag actcggatgc aggaaaacat acacctgtta aaccaatgag tactactaaa
     3301 gaccatcaca ataaagcaaa agcattacca gaaacaggta gtgaaaataa cggctcaaat
     3361 aacgcaacgt tatttggtgg attatttgca gcattaggtt cattattgtt attcggtcgt
     3421 cgcaaaaaac aaaacaaata atacaatatg acccaggtcc ttgtggcctg gtttttttat
     3481 aattacacat gcaatagatg tatttttcat ataaaataaa caataaagat acggaataaa
     3541 acttatatga ggcgataata tgaattacat tttaggaaca attttagaaa gtaaaattac
     3601 aggtgtagaa aaagcgcaaa taaatagatt gaagttgttc aaacaacacg gcatatcttc
     3661 aaaatgtgta tatgttaaat ggaatcctta ttcatacaca tatgcgaagc aacatcagat
     3721 tgaaaatgat gtatttacaa tgtatgacta ttttcaaaaa gcaatcaatt ataaaaagac
     3781 aaagcaagtt aactggatac agtattggga aaagtcatgt aggtacacat tgaaatttgt
     3841 ggaaaattca aatgatgtca gaatatatga tgaagagcaa tttgtaatgt atgctcattt
     3901 tttagataaa cagtatcatc aattaaatta tgtgaattat tttgatcata aaagaagaaa
     3961 agtaaaacgc gaattgtatg atggaagagg ctttttaagt tgttctcgaa ttttaggtga
     4021 aggacaacgg attgtactcg aaaattacta tacacctaat ggggaaatcg tcatccaaaa
     4081 atattttgac gatataaaag ggaaaaacac gctcacaaag gttatcttaa atgaagacca
     4141 gcatcaacaa ttttttgata cagaagatga attagttcaa tattttctcc atcaattatg
     4201 taaaaataat gatcaaatca tattagatcg tcctcatgaa ttaggaaatg ttatagcggg
     4261 attaaatcaa agtattccag ttattgttgt actccatagt acacatttat ccggtgccgg
     4321 taatggtata aaaagttttt ataaaacagt gtttaataat ttaacacgtt ataaagcgat
     4381 tgttgtatca acagaaaagc aatgccaaga tatttcacaa tatattgaaa ataaaatacc
     4441 agttatcaat attccggttg gctacgtggc aaatttaaag tatcaatttg acatcaatca
     4501 aaaggagaaa aatcatatca tatcaattgc tcgcctcgtt gaaaataaac aaattaaaca
     4561 tcaaattgaa gtaatcaagc aattagtaac aaaacatccc aatattcaat tgaatattta
     4621 tggacatgga aatggtttgt cagaatatcg acaacttgta gaagattatc atttatcgga
     4681 acatgttaaa tttcatggtt ttaagacgca tattaatgaa gagattgcta aagcagaact
     4741 gatgttatcg acaagtaaaa tggaaggttt tggcttagca attttagagt cgctttcagt
     4801 aggtacacca gtgatcagtt atgatgtaga ttatggtcca tcagaactga ttcaagatgg
     4861 atttaatggc tatttagtac ctcaaggtga catcaatcaa atggttgaaa aggtcgacca
     4921 attactaaat aatactcaaa aattgcaaca gttttcaatt aatagcatag aatctgcaca
     4981 acagtacaat gcaactacta tcagtacaaa gtggcaaaat attttaaact aagtcaaaag
     5041 aaaaaagcat tttcctagaa tcatctattt aacaaacctc gttcatacga acttacatgt
     5101 ttagttaaat gacataggaa aatgctattt ttgtaaaaat aaaggtaaag tacatttata
     5161 taagacgaac aaattggtcc cattgtttaa ttaacgacgc tttactatat tgttgtgctt
     5221 ttgccaaact accttttgac agtcgttgct gtacttcagg atgatcaatc acatatttta
     5281 ctttatcaaa tagggcatct tcatcatttt tagtaattaa ataaccattg aaatctgaag
     5341 taatcagttc gttaggtcca tatttaatat cataactaat aactggaaca ccatgtgcta
     5401 aagattcaag tagcgctaaa gagaaacctt ccatgttact tgttattaaa ctcaaatagg
     5461 catcgctata ttcttggtct agattgctta aaaagccgcg taagtaaaca tgattttcca
     5521 atccatattt ttgtatcaat tcatttaatt ttttactttc agaaccaaaa ccatacatat
     5581 gaagctctat ttttgggaca tacgatacta agcgtttaat taattcaatt tgttgatgta
     5641 attgtttttc aggtgaataa cgagcaacgg aaattaattt aacactgcgc tgatctaatg
     5701 tttggactgg tgtatcaatt gtttcactat agccgacagg aatattaaca actggaatag
     5761 tatggttaat acgtttttca acatctaatt tttgctgctc agtagaaacg ataattgcac
     5821 gatatcgaga taaattttca aacatcgctt tatatacatt tttaaatggc gatgaatcta
     5881 atgcatcaat atttttaatg tgtgtactgt gaagcacagc tactactggg attgactcag
     5941 gcgttaagtt gaaaataggt gctgtgtaca cattacgatc actgaaaaat aaatccccat
     6001 gttgatatag ttgtttaatg aaaaatgcgc ctaattccgt ttcattatta aagaaatatt
     6061 gtttgttagc atagtaaaca ataatttttt gtacttctgg tttgccatcc ttgtaagaaa
     6121 aatacttttc taattttgtg tcaccttctg gattatagaa aaattcacat aatgtttgtt
     6181 gtttatcaac aagaatccta ctacaactta aaaagccacg cacatcataa aaatcacgtt
     6241 ttacttttcg tctttgacta tcaaaatgat ttacataatc taatatacga tatttaggat
     6301 cttgaaaatg ggcatacatt aagaaacgct cttgatcata tattctaaag tcatgactat
     6361 tttcaacatg ttttaaagta taatgacatt catcagtcca atacgacaac cagtcaaatg
     6421 gttcattgcg ttctaaatat gttgcttctt ggaagaaatc atacatatta atatagtcag
     6481 aactagtaat ataattttgg gcatttctat ataaatatct attccatgac agaaatacac
     6541 attgcgctgg tcttcccatt tctttaaata aatttaaacg attaataatt gctttctcta
     6601 tcccagttaa attaacacct aaactattac ctacaaaata attcatttac aacaccactt
     6661 atatctattt tttataatta tatcacataa tatttaatta cttcttttaa ctggaagatg
     6721 tgtttattta taaaacaaca aattttgata tttataatga tagtagttat tcaatcaact
     6781 acgaccaata tatcattgta gagcttagga tattgattta tgactcaggc acatcaaatg
     6841 agaagattta taaaagagat atacaactct agaaggtata ataaaaacgc gcaactaatg
     6901 ttacgcgttt gaattaatca tatgatatta tttgcgatac tttaatttag cgaaagcatc
     6961 atgttgatgg atagactctt cattacgaca ttcgatatcg aaaccgtcta accaatcaaa
     7021 ttcaactaag tccgcggcaa ttaaacgaat taagtcttcg acaaaacgtg gattttcata
     7081 tgcacgctct gtcacacgtt tttcatcagg acgttttaaa atagggtata gaattgaact
     7141 tgcattagct tccattgcat ctaaaatttt atttttatag tcatcaacta tgtcttgatc
     7201 tttattaata tatgttttaa cagtgacaac accacgttgg ttgtgcgctg aatactcact
     7261 tatttctttt gaacaagggc atagcgttgt gacagttgct tcaatagtaa gttctttacg
     7321 tgtaacttta tcaccgtcaa ttgctaatcc ataagtgaca tcggcattac caactgcttt
     7381 aatatttgtg gttggactat agcgatcaaa gaaccatttc ccagaaacat caacgcctgc
     7441 cgcattttgt ttcatattcg tttgtaaagt gcgtaacacc tgataaagtg tattaaattc
     7501 aagttcaata ccattatcat agtgcttttc aacactttcg attatacggc tcatattaat
     7561 acctttttcg tcttttgtta aacttgttga aaaactaaat gtgccagctg tttgatactg
     7621 gtcaacaagt acagggtaca ctaagttttt aataccaact tcttctattt caaataaaaa
     7681 atctttatgt gtactttgta aatctgtcat ttcgttctta gtagtaggtt tcgtgccttc
     7741 aataggatct acggaaccaa agtgtttcca acgaccttct cgtgtcgata aatcaaattc
     7801 agtcattttt ttcctccgtt aagatttaaa gtgatatgtc caatatggtt cgactgttaa
     7861 aaagctgtgt tgtttaccat cgatttcagg acttgctaat tgttttaaaa atggacctgt
     7921 ttgagaagca tgtgcttcaa atgccttaat tttaagttct ttaaaatctg taatatcatt
     7981 ttgaatatca ggttctccaa gagcttcggt tgcatcatta ctgaacgcaa ctaaagttaa
     8041 acgagggcgt tcttctttag gcatgcgttc aaccgttcga attacagcgt ctgctgttgc
     8101 ttcgtgatca ggatgtactg catatccagg ataaaatgaa ataatcaatg atggatttgt
     8161 atcatcgatt aaagatttaa tcataccatc tatatgttca tagggttcaa attcgacagt
     8221 tttgtcacgt aaacccattt ttcttaaatc agtaataccg ataactttac aagcttcttc
     8281 tagttcacgc tcacgaatac ttggtaatga ttcgcgtgtt gcaaatgggg gattacctaa
     8341 atttctgccc atttgtccta gggttaaaca tgcatatgtt acaggtatgc ctttttggat
     8401 ataatttgct aatgtgcctg cagatgagaa ggtttcatca tcaggatgtg gaaatattac
     8461 taatacatgt ctttcgtcag tcatgttgat gcctcctcta taaattaaat ggtcgctcac
     8521 taatttgaag tgctgcagcg agttgacctt cgtaattaaa acctgcaatt aaaaattcat
     8581 catgctcatt gacctcaaaa tgcgttagac cttgtacata aacccaacca ccatttgata
     8641 gtttaagacc aatgcgataa ggttctttat taccaccttt tagttgtgca tgcgtatatg
     8701 ttatttgtat gtttcttaaa aaagtaccag cattaaaaac acgttgatcg aaatggttcg
     8761 cataggcccc atttgtcgtt tcaacatgca gatacacagg tttatgttca aaagaagcaa
     8821 gtaaatctat aacttcttgt tctttaattg gttccaacac gttcactcct tacactatca
     8881 atgtgtttat ctttctattt tactaaaaac tattcgataa ttgtatacga ttgctcaatt
     8941 atttataaat taattttcat gaag
//
LOCUS       DAMQYT010000099         8940 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMQYT010000099 DAMQYT010000000
VERSION     DAMQYT010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.099"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            230..1420
                     /locus_tag="PGA33_001716"
     CDS             230..1420
                     /locus_tag="PGA33_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDG6473523.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            1529..2773
                     /locus_tag="PGA33_001717"
     CDS             1529..2773
                     /locus_tag="PGA33_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HDG6473524.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVDEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(3218..4147)
                     /locus_tag="PGA33_001718"
     CDS             complement(3218..4147)
                     /locus_tag="PGA33_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDG6473525.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(4389..5768)
                     /gene="argH"
                     /locus_tag="PGA33_001719"
     CDS             complement(4389..5768)
                     /gene="argH"
                     /locus_tag="PGA33_001719"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDG6473526.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPSYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            complement(5758..6963)
                     /locus_tag="PGA33_001720"
     CDS             complement(5758..6963)
                     /locus_tag="PGA33_001720"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDG6473527.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            7314..8645
                     /locus_tag="PGA33_001721"
     CDS             7314..8645
                     /locus_tag="PGA33_001721"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDG6473528.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
BASE COUNT         2962 a         1405 c         1619 g         2954 t
ORIGIN      
        1 tagtaaaata tacttttgat taagtgtaaa attatcaatt cttttgcttg taatcattta
       61 agtaattatg tcattataaa tgtaagggtt ttcaaaatag acaaatttaa tgaaacttag
      121 tgattatttc atcttttttg gtggaaaagt atctgctttg aaaactaatg taattatttg
      181 gataatgttt catgtataat agttataaat gatgaaaagg ggagatatga tgactaaatc
      241 tgaaaaaatt attgagttaa caaatcatta cggagcacat aattatttac cattgccaat
      301 tgtcatttca gaagctgaag gggtatgggt taaagatcct gaaggcaata aatatatgga
      361 tatgttatct gcatattccg ctgttaacca aggtcataga catccgaaaa ttattcaagc
      421 attaaaagat caagctgata aagtgacttt agtttcacgt gcttttcata gtgataactt
      481 aggtgaatgg tacgaaaaaa tttgtaaact ggcaggtaaa gataaagctt taccaatgaa
      541 tacaggtgct gaagcagtag aaacagcttt gaaagcagca cgacgctggg catacgatgt
      601 taaaggaatt gagccaaata aagcagaaat cattgcattt aatggtaact tccatggtcg
      661 aacaatggcg ccagtttcat tatcttcaga agcagaatac caacgtggtt atggtccgtt
      721 attagatgga tttagaaaag ttgattttgg agatgtagat gcattgaaag ctgcaattaa
      781 tgaaaatact gcagcagttt tagtagaacc aattcaaggt gaagcgggta taaatatacc
      841 gccagaagga tatttgaaag caattagaga attatgtgat gaacataatg tcttatttat
      901 tgctgacgaa atccaagcag gattaggtcg ttcgggtaaa ttatttgcta cggattggga
      961 taatgtaaaa cctgatgtct atattttagg taaagcacta ggtggtggag tcttcccaat
     1021 ttctgttgta ttagcagata aagaagtatt agatgtcttt acacctggct cacatggttc
     1081 aacatttggt ggtaatccac ttgcttgtgc tgcatcaatt gctgcattag atgttatcgt
     1141 tgatgaggat ttaccaggcc gctctttaga attaggagat tattttaaag aacaattaaa
     1201 gcaaattgat catccatcaa ttaaagaagt ccgtggacgt ggtttgttta taggtgtgga
     1261 acttaatgaa agtgctagac catattgtga agctttgaaa gaagaaggct tattatgtaa
     1321 agaaacgcat gatactgtca ttcgttttgc accaccatta attattacta aagaagaatt
     1381 ggaccttgca cttgaaaaaa taagacatgt atttcaataa tgtcaaaaag ttgtaacaat
     1441 cagtttgtaa gggctttaaa aaggaatacg ttatgttaca atatgtttga aagcgaaatc
     1501 attaggtaaa gaagaggcga aaaggatcat gactgagaac aataatttag taacttctac
     1561 tcaaggaatt attaaagaag cattgcataa attgggattt gacgaaggaa tgtacgattt
     1621 aattaaagaa cctttaagaa tgttacaagt gcgtatccct gtacgaatgg atgatggcac
     1681 agtaaaaaca ttcacaggtt accgtgcgca acataatgat gctgttggac caacaaaagg
     1741 gggcgtgcgt ttccacccag atgttgatga agaagaagta aaagcattat caatgtggat
     1801 gactttgaaa tgtggcattg taaacttacc atacggtggt ggtaagggtg gtatcgtttg
     1861 tgatccacgt caaatgagca ttcatgaagt tgaacgttta tcacgcggat atgtaagagc
     1921 aatttcacaa ttcgtaggtc cgaacaaaga tattccagca ccagatgtat ttacaaactc
     1981 acaaattatg gcttggatga tggatgaata tagtgcatta gataaattta attcaccagg
     2041 tttcatcaca ggtaaaccaa ttgtattggg tggttctcat ggacgcgaca gatcaactgc
     2101 actaggtgta gttattgcaa ttgaacaagc tgcaaaacgt cgtaatatgc aaattgaagg
     2161 tgccaaggtt gttattcaag gtttcggtaa tgccggaagt ttcttagcta aattcttata
     2221 tgatttaggt gcaaaaattg taggtatctc tgatgcttac ggtgcattac acgatccaaa
     2281 tggcttagat atagattatt tattagaccg tcgtgatagt tttggtacgg taacaaattt
     2341 atttgaagaa acaatctcaa ataaagaatt gtttgaatta gattgtgaca ttttagtacc
     2401 agcggctatt tcaaaccaaa ttacagaaga caatgcacat gatattaaag ctagtatcgt
     2461 tgttgaagct gctaatggac ctacaacacc agaagcaaca cgtattttaa ctgaacgtgg
     2521 tatattatta gttccagacg tattagcaag tgctggtggt gtaacggttt cttacttcga
     2581 atgggtacaa aataatcaag gttattattg gtctgaagaa gaagttgatg aaaagctacg
     2641 tgaaaaatta gaagcggcat ttgatacgat ttacgaattg tctcaaaacc gaaaaataga
     2701 tatgagactt gcagcatata tcataggtat taaacgtaca gcagaagcag ctagatatcg
     2761 tggttgggca taattaatta tcatatgtga tttaacgagc ttgggacaga aaacaaagcc
     2821 ctaagctcgt taattttatt ttagcagtag ttgactgtaa aacaatgccc gtgtaacacg
     2881 ctcttttcaa aaatagtcgg ggccccaaca cagaggctgg tggaaagtca gcttacaata
     2941 gtgtgcaagt tggcggggcc ccaacacaga ggctggcgga aagtcagctt acaatagtgt
     3001 gcaagttggg gtggagcccc gacacagaga aattagctcc tcaatttcta cagacaatgc
     3061 aagttggcgg gacgacgaaa taaattttgc gaaaatatca tttctgtccc actcccaaaa
     3121 agacagcagt aggataattt caatttggaa atatcttact gctgtttttt atagtaatag
     3181 ctatattttt gtatttatgt tttctagttt aacattacta cttaatgact tctttatatt
     3241 tatcagcgaa atttgtaaag acaccatcaa cgccatattt atttaatcgt aacatatcag
     3301 ctttttcatt cactgtataa ggatgtacta taaatcctaa gtcttttaaa tgatgggtat
     3361 tttgttcagt taaatctgta taatcaggac ctaatccaat cgcataagag cgtatctctt
     3421 ttaagcgttg gtcgttaaat tgttgtagtt cacctttatc aactaatttt actaatggca
     3481 catgcttatt ttgacgatga attttcttta aactttcgtc agaaaatgat tgaatcatta
     3541 catgtccatt ttttaattta ttgttattta aaaggtgatg ctttttcaat gaagctaata
     3601 attgttcttc cattcctggg tatacatcag gtgactttgt ttcaatataa tagtttgcat
     3661 tcgggccata acgttctaaa atttcatcta aagtgggtac tttagcattt ttataacttg
     3721 ctcttgcgta ttttggatat tttttattaa accaacttcc tgcatctaac tgttttaatt
     3781 catcaagggt ataatcctca actttaccgt gtccatttgt tgtacggtta acagtttcat
     3841 catgcatagc aactaaatgg ccatctttgg tacgttgtaa atcaatttcg atataagatg
     3901 cttttaactc attatgactc ttatcatatg cttgaaacgt atgctcgggt gcatagccac
     3961 ttgcgccacg atgtgcgata gtagtgaatc gctcattcgt taaatttgta tgccattgaa
     4021 tagcctgagg tttatttgca atttgatttg tttgttcagc gccagcagta ggtactgata
     4081 aaaatcccat agtaaaaaca gcagaagcag ccataaattt agtgaagctt ttcgaagagt
     4141 tagtcatagt ctttatcctt tcatctcttt atttgtagtc taacaatttt aaaatactac
     4201 aaaaacctgc ttgtgaaaac gctttacaca tttcttttac aatttataac atttttgtaa
     4261 tataaattta aaagaattat caaacatttt aggaatctta aaaatgtgat gttcaacaag
     4321 acgaagagag cattaaaata cccacaatgt aattttaata gacattgtgg gtaggtagat
     4381 taacgtattt attgtgatag taattgttta gcaacatcaa gttgttgttt gaccgatgat
     4441 tgacctgttg aaccgtaact ttgacgtcgt tttaaacaat tttcaggctg caaataatcg
     4501 taaatatcgg catcaatact agaatgatgt tgttgatatg ttgctaaagg aacatctaat
     4561 aaataatgac cttgttgtat acattctaag acgatttttc ctacaatttc atgtgcagtt
     4621 ctaaatggaa tatttttagt tactaaataa tctgctagtt ccgttgcatt tgaaaaatct
     4681 tctttaacag tttgattgag tcgttcttta ttaattgtca tcgtttgaat cataccttcg
     4741 aaaatacgta aagaaccttt aattgtatgg acagcatcga ataaaccttc tttatcttcc
     4801 tgcatatctt tgttatatgc tagaggtaat ccttttaaag tcataagcat gctcattaaa
     4861 tgaccagtcg ttcgaccaac tttacctcta attaattctg ccatatcagg atttttcttt
     4921 tgtggcataa tagatgagcc agttgaaaat gcatctgata atgtaatgaa tttagcttcg
     4981 tctgtggacc agaaaataat ttcctctgca aagcgtgata agtgaaccat cgttaaagaa
     5041 atattatgca atgtttcaat aatatagtct ctgtcactaa cagcatctag gctattctca
     5101 tagagactgc caaagttcaa caatgctgtt gtctcgtgtc tatcgatagg gtatgtggta
     5161 ccacttaagg ctgctgcacc taaaggatta atatcgattc gttttaaact atcttcaaat
     5221 cgttgttggt ctcgttgtaa catccaaaaa taagtcataa tatgatgtgc aaatgaaatt
     5281 ggctgtgcac gctgtaaatg agtataacta ggcataattg tatcaacatt attggaagcg
     5341 atgtctacaa ttacactttg taacgactta attaatgcga tgatatcttg cacttgtttc
     5401 ttagtgtaca agtgcatgtc tgttgcaact tgatcgtttc tactgcgtcc agtatgcaac
     5461 ttaccaccag catcaccgat acgtttaatt aattcatgtt caatatttaa atgaatatct
     5521 tctaatgatg cactaaattg aatttgatct tgatgataat catgttgaat agattttagt
     5581 ccttgtataa tttgttcgct gtcttgttga ctaataatgc cttgattcgc aagcatagtt
     5641 gcatgtgcaa tgctgccttc gatatcttga tctatgagcg tttgatcaaa agtaatggat
     5701 gcgttaaagt cgtcaaccca ctcttcaggt tgtacttcaa atctaccgcc ccaagcttta
     5761 ttgctcattg ctatagcctc catgcaacat cgcatttact tgagtaggta aaccatagat
     5821 atcgataaag ccaacagcag cgtcttgatt aaatgcatct tcttttgtat aagttgctaa
     5881 tttttcatca tataatgtgt aaggtgattt tctaccattc acgatggcat tacctttgaa
     5941 taatttaatt ctgacatcac cacttacgta ttgctgagta ctatcaataa ataatttcaa
     6001 gctatcagtt aaaggtgaga accaaagtcc attgtatagt tgttcagcaa attgcttctc
     6061 aatgattggt ttaaagtgtg cgacatcttt cgttaacgta atcgtttcta atgctttatg
     6121 cgcttttaaa ataacttctg cagcaggtgc ctcataaatt tctcttgatt tgatacctac
     6181 aagtctattt tctacatggt caattcttcc gataccatgc ttaccagcta atgcattcaa
     6241 cgttaaaatt aaatcgtcta attcatatgt tttgccatca atttgaactg ggatgccttt
     6301 atcaaacgtt aaaatgattt catcagcagt atctggtgtt tcttctaaag catttgttag
     6361 atcgaacgca tcctctggtg gcgcagcata aggatcttct aaaataccac attcattcgc
     6421 tctgccccat agattttgat cgatagaata aggtgaatca tggttgattg atacagggat
     6481 attatgttta attgcataat cgatttcttc ttcacgactc catgcccact cacgtacagg
     6541 tgcgaatgct ttcaatgatg ggtttaatgc tttaatggca acttcgaaac gtacttggtc
     6601 attcccttta ccagtacaac catgtgcaat acctactgaa tttgttttct cagcaatctc
     6661 tactaatttt ttagcgatta atggtcttga taaagctgaa actaatggat atgcattttc
     6721 atacattaaa tttcctttga ttgcataact tacatactca tcactaaatt cttttgttgc
     6781 atcaataata tgacattcaa ctgctcccat atctaaagct tttttataaa cgatgtctaa
     6841 atctttacct tcaccaacat ctaggcaaca agctacaacg tcgtatcctt tgtcgataag
     6901 ccattgaacg gccacacttg tatctagtcc tcctgaatat gctaaaacaa ttttctcttt
     6961 cataaaattc acctcattgt agttatctga atatttcgta tgaacacatg atagcaaact
     7021 tttaatgaaa ataaaaccat tattttacat atttattcat aaaatgcgat agagatagtt
     7081 agagttgtaa gtgtaaatgc ttatttatca atgtttaaaa gtattttaaa aaacatgcaa
     7141 attgtataaa aaatgtttga gtgtttgacg ccagtttgaa atgtttgttg aaaaataggt
     7201 gtgaaacgtc acataaatat caattttatt gattaagtac aaacttgtga attgaatcgc
     7261 aaacgtaaac ttagtaaaat taagatatta aatgaaacag gaggctttta tagatgactc
     7321 atattcaatt agattttagt aaaacgttag aatttttcgg tgaacacgaa ttaaaacaac
     7381 aacaagaaat tgttaaatca attcacaaaa caattcatga aggtactggt gcaggtagtg
     7441 acttcttagg ctgggttgat ttaccagttg attacgacaa agaagaattt tcaagaattg
     7501 ttgaagcatc aaaacgcatt aaagaaaatt ctgatgtttt agtagtcatc ggtattggtg
     7561 gttcttactt aggtgcacgt gcagcaatcg aaatgttaac gtcatcattt agaaacagca
     7621 atgaataccc tgaaattgta tttgttggta atcacttatc atcaacatat acgaaagagt
     7681 tagttgatta tttagcagac aaagatttct ctgtaaacgt tatttctaaa tctggtacaa
     7741 ctacagaacc agcagttgca tttagattgt tcaaacaatt agttgaagaa agatacggta
     7801 aagaagaagc acaaaaacgt atatttgcaa caacggataa agaaaaaggt gctttaaaac
     7861 agttggctac aaacgaaggt tatgaaacgt ttatcgtacc tgatgatgta ggtggaagat
     7921 attctgtttt aacagcagta ggattattac caattgcaac agctggaatt aacatcgaag
     7981 ctatgatgat tggtgctgca aaagcacgtg aagaattatc ttcagataaa ttagaagaaa
     8041 acattgcata ccaatatgcg acaattcgaa acattttata tgcaaaaggt tatacaacag
     8101 aaatgttgat taactatgaa ccatctatgc aatactttaa tgaatggtgg aaacaattat
     8161 ttggtgaatc agaaggtaaa gacttcaaag gtatctatcc ttcaagtgcc aactacacaa
     8221 ctgatttaca ttctttaggt caatatgtac aagaaggccg tcgcttctta ttcgaaacag
     8281 ttgtaaaagt aaatcatcct aaatatgata ttactattga aaaagatagt gatgatctag
     8341 acggattaaa ttatttagct ggtaaaacaa tcgacgaagt taacacaaaa gcattcgaag
     8401 gtacattatt agcgcatact gatggtggtg ttcctaacat ggtagtgaac attccacaat
     8461 tagatgaaga aactttcggt tacgtcgtat acttcttcga acttgcttgt gcaatgagtg
     8521 gataccaatt aggcgtaaat ccatttaacc aacctggtgt agaagcatat aaacaaaaca
     8581 tgttcgcatt attaggtaaa cctggttttg aagacttgaa aaaagaatta gaagaacgtt
     8641 tataaaatac attacttcaa agattagtga agtttgaaaa gatagaacta gacgttaact
     8701 atttaaagca tattttcgag gttgtcatta caaatgtaaa aatgtaatga caacctcgtt
     8761 tttatttata tgcaagaact aggttactag ctaatgtgac aagatgttaa gagaaaatta
     8821 aagataaaat aatatctgcc ttacaataat attgttatac tactagagac tgatttatta
     8881 gcatgattac atgttaatgt ttctttactt agtaattaac tttataatgt aagaataatt
//
LOCUS       DAMQYT010000100         8934 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMQYT010000100 DAMQYT010000000
VERSION     DAMQYT010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8934
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.100"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..516
                     /locus_tag="PGA33_001722"
     CDS             <1..516
                     /locus_tag="PGA33_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HDG6473529.1"
                     /translation="NLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQRAEAIVKRKS
                     LMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVKERIMSAAAI
                     QGSQFIGKRISNAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYFMNKIGKDHI
                     QKCENVIKNVM"
     gene            670..936
                     /locus_tag="PGA33_001723"
     CDS             670..936
                     /locus_tag="PGA33_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDG6473530.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            939..2657
                     /gene="ptsP"
                     /locus_tag="PGA33_001724"
     CDS             939..2657
                     /gene="ptsP"
                     /locus_tag="PGA33_001724"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDG6473531.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
     gene            complement(2894..3127)
                     /gene="nrdH"
                     /locus_tag="PGA33_001725"
     CDS             complement(2894..3127)
                     /gene="nrdH"
                     /locus_tag="PGA33_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDG6473532.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHHIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            3325..4686
                     /locus_tag="PGA33_001726"
     CDS             3325..4686
                     /locus_tag="PGA33_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDG6473533.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            4683..5702
                     /locus_tag="PGA33_001727"
     CDS             4683..5702
                     /locus_tag="PGA33_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDG6473534.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFGISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            5835..6497
                     /locus_tag="PGA33_001728"
     CDS             5835..6497
                     /locus_tag="PGA33_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDG6473535.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
     gene            complement(6846..8543)
                     /locus_tag="PGA33_001729"
     CDS             complement(6846..8543)
                     /locus_tag="PGA33_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDG6473536.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
     gene            complement(8543..8761)
                     /locus_tag="PGA33_001730"
     CDS             complement(8543..8761)
                     /locus_tag="PGA33_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDG6473537.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            complement(8790..8912)
                     /locus_tag="PGA33_001731"
     CDS             complement(8790..8912)
                     /locus_tag="PGA33_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473538.1"
                     /translation="MNDMMNLVINQIVIKLHYRDDWNEFIDIYSNTILLGLTIN"
BASE COUNT         2949 a         1312 c         1618 g         3055 t
ORIGIN      
        1 aatttaacaa ataaaatcgg taattcagtc tttaaaatag aaaatgttga cggaaaaggt
       61 gcaatgccaa cgacgattca agaattgaga gaaagacgac aacgtgctga agcaattgta
      121 aagagaaagt ctttaatgtc atcaacaatg agcgttgttc caattccggg tttagatttt
      181 ggtgttgatt taaaattaat gaaagatatt atcgaagatg ttaataaaat ttatggttta
      241 gatcataagc aagttaatag ccttggggat gatgtgaaag aaagaattat gtctgcagca
      301 gcaattcaag gtagtcaatt tattggtaaa agaatttcaa atgcattttt aaaaattgta
      361 attagagatg tagctaaacg tactgctgca aaacaaacaa aatggtttcc tgttgtagga
      421 caagctgtgt ctgcatctat tagttactat tttatgaata aaattggaaa agatcacatt
      481 caaaaatgcg aaaatgttat taaaaatgtc atgtaggtgc tataatagtt ttgcaatttg
      541 caaattttac tgaaaccggt tttaaacgaa ttgaatttaa agcatggttt tggtaaagtt
      601 aatgtataaa actaagttag tattgtaata atattgaaga ttctaactat acgaaggaga
      661 aatgtaatta tggaacaaaa ttcatatgta atcatcgacg agactggtat tcacgctaga
      721 ccagcaacaa tgttagtaca aacagcttca aaattcgatt ctgatattca attagaatat
      781 aacggtaaga aagtaaactt aaaatcaatc atgggtgtta tgagccttgg tgttggtaaa
      841 gatgctgaaa ttacaattta tgctgacggt agtgatgaat ctgacgccat tcaagcaatc
      901 agtgacgtct tatcaaaaga aggattgact aaataatcat gtctaaatta attaaaggta
      961 ttgccgcatc tgatggtgtc gcaattgcta aagcttattt attagttgag ccagacttaa
     1021 cattcgacaa aaatgaaaaa gtcactgatg ttgaaggaga agttgcaaag ttcaatagcg
     1081 ctatcgaagc ttctaaagtt gagttaacta aaattagaaa taatgcagag gttcaactag
     1141 gtgctgataa agctgctatc tttgatgcac atttattagt tttagatgac cctgaattaa
     1201 ttcaaccaat ccaagataag attaaaaatg aaaacgctaa tgctgctaca gcattaacgg
     1261 atgtaacaac acaatttgtt acaatttttg aatctatgga taacgaatac atgaaagaac
     1321 gtgcggctga tattcgcgac gtttctaaac gtgtgttatc acatatttta ggtgtagaat
     1381 taccgaatcc gagtatgatt gatgaaagcg ttgttattgt agggaatgac ttaacgccat
     1441 ctgatactgc tcaattaaat aaagaattcg tacaaggttt tgctacaaac attggcggaa
     1501 gaacaagtca ctctgcaatt atgagtcgtt ctttagaaat tccagcaatt gttggtacaa
     1561 aatcaattac tcaagaagtt aaacaaggcg acatgattat cgtagatgga ttaaatggtg
     1621 atgtaatcgt taatccaact gaagatgagt taatcgctta tcaagataaa cgtgagcgtt
     1681 attttgctga caagaaagaa ttacaaaaac tacgtgatgc tgatactgtt acagttgatg
     1741 gtgttcacgc agagcttgct gcaaatattg gtacacctaa tgatttgcca ggtgttattg
     1801 aaaatggtgc acaaggtatc ggcttatata gaactgagtt tttatatatg ggtcgtgacc
     1861 aaatgcctac agaagaagaa caatttgaag cttataaaga agtattagaa gcaatgggcg
     1921 gtaaacgtgt tgttgtacgt actttagata taggtggaga taaagaatta tcatacttaa
     1981 acttgcctga agaaatgaat ccattcttag gttaccgtgc gattcgttta tgccttgcgc
     2041 aacaagatat tttcagacca cagctacgtg cattattacg tgcatcagtt tatggtaagt
     2101 taaatatcat gttcccaatg gttgcaacaa ttaacgaatt tagagaagct aaagctatat
     2161 tattagaaga aaaagaaaac cttaaaaatg aaggtcatga catttcggat gatatagaat
     2221 taggaatcat ggtagagata cctgcaacag cagcattagc tgatgtcttt gctaaagaag
     2281 tagatttctt cagtatcggt acaaatgatt taattcaata cacattagct gctgaccgta
     2341 tgtcagagcg tgtatcatat ctataccaac catataaccc ttcaatctta cgtttagtta
     2401 aacaagttat tgaagcgtca cataaagaag gtaaatggac aggtatgtgt ggtgaaatgg
     2461 ctggagatga aacagctatt ccattattgc ttggtttagg tttagatgag ttctctatga
     2521 gtgcaacgtc tattctgaaa gcaagaagac aaattaatgg tttaagtaaa aatgaaatga
     2581 ctgaacttgc taaccgtgca gtcgactgtg caacgcaaga agaagttatt gaattagtta
     2641 acaactacgt aaaataatta attctttcga aagacagaat tataaataaa cccgaagtat
     2701 tcattgtaag taattatgaa tacttcgggt tcttttttat gatttatgtt aatttcaatg
     2761 gaatgttgtt gctaactaat taaacaacta tgtgtcatta acaaaatgta gttctttaat
     2821 taatgttagt taatttaaaa atcgatattt aatttatgat agctataaat gatgaagttg
     2881 atttagatga aaattaatct tggatattta atactttgtt gatttcatca agatcaacat
     2941 ggtacattgg attgccattc aacaaaataa acggagttga aaaagcatca aaatctatca
     3001 tttcgtttcg atattgttga ttgttgatat ttctctcttc aaaatcaatg tgatgctcat
     3061 ttagataatt ttttacaaat gtacaaggtg gacaatcatt ctgcgtataa acgattattt
     3121 ctgacatgta tcatgctcct tcgtgcttta ttctaatgta atatacacaa ttatacattg
     3181 aaattcaaat ttgtgaacac attgtgaact gacataaatt atacacaatt tcatagcgca
     3241 taatgtttta aaaatacaca ttatagcaat tataattaag ttcattgtgt tgtcatcaaa
     3301 aaaagaaaaa ggtgatgttt ttaaatggat acagttgaaa tcagtcggtt tttgacagct
     3361 atgactttag cagttcatat catttttgca acgattggtg ttggtatgcc tttaatgttc
     3421 gcaattgcag aatttttagg tattcgcaaa aatgatcttc aatatatagc tatggccaaa
     3481 agatgggcta aagcttatac aattactgta gcagtgggag ttgttacagg tacaattata
     3541 ggacttcaat tatcattgat ttggcctaca tttatggaaa tgggtggaca cgttattgca
     3601 cttccattat ttatggaaac atttgcgttc ttctttgaag ctattttctt aagtatatat
     3661 ttatatactt gggatcgttt taaaaataaa tggacacatt tcttaattag tataccagta
     3721 attattggtg gctctttctc agcattcttc attacttcag tgaattcatt tatgaatacg
     3781 cctgcaggtt ttgagttgaa gaatggaaag atggtcaatg ttcaacctat agaagcgatg
     3841 tttaacccat cgtttatagt tcgatcattt cacgtaatta ctacagccgg tatgacgatg
     3901 gcatttgtta ttgcatcaat agcagctttt aaattattgc gtaatcgtca acctaaagat
     3961 actgtctacc ataagaaagc tttgaaaatg tctatgatag ttggattctt ttcaacatta
     4021 ctttctatgt tggcagggga tttatctgca aaatttttgc ataaattcca acctgaaaaa
     4081 ttagcagctt atgaatggca tttcgataca tcttcccatg ccaaattatt attatttggt
     4141 gtgttagatg aaaagactca gcaagttaaa ggtgcgattg aattacctgg actattgagt
     4201 ttcttagcag ataatagtgt caaaactaaa gtacaagggt taaatgattt tccaaaaagt
     4261 ttacatccgc ctatgatcgt ccattatttc tttgatttaa tggtaacgat gggaatttta
     4321 tgttttgtca tttcaggtgt ttatgtctta actttaatgt ttaaaaagct tagaaagttt
     4381 tctactcata aatggatgct ttatggaata ttattaacag gcccagcttc aatgctagct
     4441 atagaatttg gatggttctt aacagagatg ggtagacagc cttggattgt tcgtggttat
     4501 atgcgcgtgg cagaagcagc aacacaagca ggcggaataa ccttcgttac aattttattt
     4561 ggcatattgt acatcatttt aatgtataca tgtgcatacg tattaattcg tatgtttaaa
     4621 aataaaccgg cgtatgaaga tgtaaatcgt ttagccaaga agcaaggagg agaaatagaa
     4681 aaatgattta tgcatttata ggtataacag tgttatggtt gtttttattt tgctatatca
     4741 ttattgcttc tatagatttt ggggcaggtt tcttcgcatt gcattcaaag ttaactggtg
     4801 atgaaaagaa aattaatcac ttaatttcac gttatttaaa cccagtttgg gaagttacga
     4861 atgtattctt tgttttcttc ttcgtaggat tcgtaggttt ctttccagag tcaatcaaat
     4921 atttaggcac ggtattgtta ataccaggtt caatagcact gattatgata tcgttgagaa
     4981 acagttttta tgcatttgaa aattatggtc aagatacaaa attagcatgg atgatcatgt
     5041 atggggtaag tggattatta attccagctt cattatctac tgctttaact attacagaag
     5101 gtggctatat taatgttcga aacaatgtta tcgatctaga ttgggtgcag ttattattaa
     5161 gcccatttgc ttggtctgta gtattcttgg caattatttc agttttatat atttcatcag
     5221 gatttttgac atattatgct aaaaaagcaa atgacgaacc agcatataat ttaacaagac
     5281 aatggcacat atttttaggg ccgccgatga ttatcatttg tttattcgta tttctatcat
     5341 tacgcattca aaattctgaa catttttatt cagctgtttt tgactattgg tggatgtttg
     5401 gtataagttt cttattcttt gcattagctt cattgttaac attctttaag aaaaaacatg
     5461 gtttggcttt tgtatttgtt attttacaaa tgatgttcgc gttctttggc tatggaatta
     5521 gtaaattgcc atacctttta tatccgtttg taaaaattac agatgcatat gttaatccag
     5581 aaatgggctg gacattagtg attgtcttta ttttaggttt acttttatta cttccatcgt
     5641 taatattatt attaagatta tttgttttcg acaaagaata tgttgaagga aagaaatcat
     5701 aagttgagac gataccccaa ctttcattta cttgcattac tgcatttaga taatttagtt
     5761 ggggtactca tttataataa ttttaaattt atattttcaa tgaattgatg aaaatcattt
     5821 aatggagggc gagtatgggt aaagaatatg tagtcatcgg tctaggtcgt tttggaggta
     5881 gtatcgttcg tgagttgaat gcattagaca tggatgtaat ggccatcgac catgatgaaa
     5941 atagagtgaa tgaatatagt gatatcgcta ctcatgcggt tgttgcagac actacagatg
     6001 aagcagttat gaaaagttta ggtatccgta actttgatca tgtcattgtg gcaattggtg
     6061 aaaatattca atcaagtacg ttgacgactt taattttaaa agagttaggt gtaaaaaaag
     6121 taactgctaa agcacaaaat gattatcatg caaagatttt aaataaaatt ggagcagata
     6181 cggttgtgca ccctgagcgt gatatgggta gacgtattgc gcataatgtt gcgagtgcaa
     6241 gtgtacttga ttatcttgag ttggcagacg agcattctat tgtagaattg aaagcaactg
     6301 aaaagatggc ggggcagtct atcattgatt tagatataag agcacaatat ggaattaaca
     6361 ttattgcaat taaacgaggc aaagagttca tcatttcacc aaatccaaat attaatttag
     6421 aaataggtga tattttaatc atgattggac atgataatga tttaaatcgc tttgaaaaaa
     6481 atattgcgac gagataatcg ggagtgggac agaaatgata ttttctcaaa atttatttcg
     6541 tcgtcccacc ccaacttaca ttgtctgtag aaattgggaa tccaatttct ctttgttggg
     6601 gcccacaccc caactcgcat tgcctgtaga atttcttttc gaaattctct atgttggggc
     6661 ccctgactag aattgaaaaa agcttgttac aagcgcattt tcgttcagtc aactactgcc
     6721 aatataactt tgtagagcat tgaacattga tttatgtctc aagctcatca tatataaaga
     6781 aaaagctact aacaatgggt ggtgagttta attaaaactt cacttcaaag ttagtagctt
     6841 tttgattatt tattgtttga ttctttttgt tcgtttacct tcataatgac tggtaaaatc
     6901 attggttttc tagctgtttt ttcaaataaa taaggttgta atgtttcaat gatagaagat
     6961 ttaatctgat gccattgaat atctttattt tgatttaact tactaataac atcagttttg
     7021 attttgcgtt gtgcatcata aattaattga cctgattccc tcatatatac aaatcctcga
     7081 gaaataatgt ctggaccaga aagtaattta tttgtattaa aatcaatact aacaacaacg
     7141 ataactaaac cttcttcaga taatagctta cggtctctta ttacaacatt accgatatca
     7201 ccgataccac taccatcaac aagtacatta ccagatggaa tgcgaccagc tttacgtgct
     7261 gaatcgtgtg ttaaagctaa gacatctcca atatcaaaga tgaagacatt atcttcttca
     7321 acgccgcatt caacaccagt ctcaccatgt gcttttaaca tacggtattc accatgaata
     7381 ggtaagaaat atttcggctt gattaatcga agcattaatt gttgatcacc ttgagaacca
     7441 tgccctgaag tatggatgtt agaaatcttg ctatggataa catctgcacc agctttatac
     7501 aaggaattaa tagttctgtt aatacttttt gtattacctg ggataggtga tgaactaaat
     7561 acaacggtat cttcaggtat aattttaatt tgcttatgag taccattagc aattctagat
     7621 aatgctgcca ttggttcacc ttgtgaacca gtacatagta tcaataactc atgcttcggt
     7681 acggtattaa ttttattagg ttcaataaat gtttcaggtg gtgctttaat ataaccaagt
     7741 tccataccta ttttaatatt gttttccatc gaacgaccga acgtaacaat tttacggtta
     7801 tttttgatag cagcttcaac tgcttgttga actcggtaaa tattagaagc gaaggtagca
     7861 aatataatac gacctttaca attacggaag atcttatcta cgttttgacc aacttcacgt
     7921 tcgcttaaag taaaatcagg cacaagtgaa tttgttgagt ctgaaagtaa acatagaacg
     7981 ccttcttcgc ctaattgagc cattttagca atgtttgctg gtttgcctac aggtgtaaaa
     8041 tcaaatttaa agtcaccggt atgaactact tttccttcag gtgtatctac gatgacgcca
     8101 taagtttcag gaatactatg tgtagttaag tagaaagaaa tcgtaaagtg cttagattta
     8161 atcacactgt cctcattgat ttcatttagt ttagcagtac gtaataaatg atgttcttca
     8221 agtttattac ggattaaacc taatgctaaa ggaccaccat aaataggtat attaagttgt
     8281 tttaatagga agggcacacc gcctatatgg tcttcgtgac catgtgttat aaataggcca
     8341 acaattttat cttggttttg aactagatat gtgtagtcag gtataacata atcaatccct
     8401 aataagttat catcagggaa tttgatacct gcatcgataa tgacaatttc gtctttatac
     8461 tcaactgcat aagtattttt accgatttca cctagacctc caagtgcata tacacctact
     8521 tcatttggat gtaattgttt cattatttag caatctccac attaaagtgt tctgagtttt
     8581 ctttttcgta atctaaatgt gcgccctcta atttagtgat aaattcgata ttaaaattac
     8641 gatctttcaa gtaacgacgt acttgttctt ctgtttgagc ttcaacataa agtgattgtg
     8701 tattttcacg cacaattacc tcgtctctgt tatgttgata aaaaacttta aatactgcca
     8761 tgttaaaaat tcctcctaag aatgtttgtt taatttattg ttaaccctag taaaatcgta
     8821 ttggagtata tatcgataaa ttcattccaa tcatctctat aatgtaactt aataacgatt
     8881 tggttaataa ctaggttcat catgtcgttc attttaaaaa attagtgaaa taac
//
LOCUS       DAMQYT010000101         8906 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMQYT010000101 DAMQYT010000000
VERSION     DAMQYT010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.101"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..191)
                     /locus_tag="PGA33_001732"
     CDS             complement(<1..191)
                     /locus_tag="PGA33_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDG6473539.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEG"
     gene            complement(203..496)
                     /gene="csoR"
                     /locus_tag="PGA33_001733"
     CDS             complement(203..496)
                     /gene="csoR"
                     /locus_tag="PGA33_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HDG6473540.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            662..2146
                     /gene="cls"
                     /locus_tag="PGA33_001734"
     CDS             662..2146
                     /gene="cls"
                     /locus_tag="PGA33_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDG6473541.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            2174..2821
                     /locus_tag="PGA33_001735"
     CDS             2174..2821
                     /locus_tag="PGA33_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDG6473542.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNCHD"
     gene            complement(3634..4506)
                     /gene="yidC"
                     /locus_tag="PGA33_001736"
     CDS             complement(3634..4506)
                     /gene="yidC"
                     /locus_tag="PGA33_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDG6473543.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(4592..5233)
                     /locus_tag="PGA33_001737"
     CDS             complement(4592..5233)
                     /locus_tag="PGA33_001737"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDG6473544.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            complement(5235..6026)
                     /gene="thiM"
                     /locus_tag="PGA33_001738"
     CDS             complement(5235..6026)
                     /gene="thiM"
                     /locus_tag="PGA33_001738"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDG6473545.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVIT
                     GKEDVIVQDNKAFVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            complement(6010..6840)
                     /gene="thiD"
                     /locus_tag="PGA33_001739"
     CDS             complement(6010..6840)
                     /gene="thiD"
                     /locus_tag="PGA33_001739"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDG6473546.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            complement(6833..7522)
                     /gene="tenA"
                     /locus_tag="PGA33_001740"
     CDS             complement(6833..7522)
                     /gene="tenA"
                     /locus_tag="PGA33_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HDG6473547.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     regulatory      complement(7603..7705)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(7909..8604)
                     /gene="sceD"
                     /locus_tag="PGA33_001741"
     CDS             complement(7909..8604)
                     /gene="sceD"
                     /locus_tag="PGA33_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HDG6473548.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGTNGADMSAQAPATNNV
                     APSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKSSEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
BASE COUNT         2700 a         1611 c         1461 g         3134 t
ORIGIN      
        1 ccttcatcat agatttcttt ttcaatacta ttcaaatttg ctggtgtctc atacgataca
       61 gttacttgac catctattat attaatattc acttgatgaa cacctatcat atttgaaatg
      121 cgttctgtca attgacttac ttgttcttct gtttcaattc ccgctgtgta aatcgtattt
      181 tgatgtatca tcttaaactc ctttagtctt taatcaattt ttgaaaagtc actaataact
      241 cttccattgc ctcttcctga gcaccttgat taactttatt cataatacaa cttttcatat
      301 gttgttctaa taactttatc gcaacactgt ttaacgccga acgtgtcgct cttatttgcg
      361 taaggacatc atcacaatag acatcttctt caatcatgcg attaatcgct ctcacttgtc
      421 cttcaattcg atttaaacgt gatttaagat tcgtttttat ttgttcagaa tgatgtgcat
      481 tatcttgttc agtcatagca ttcactcctt catttacatt ccttactata ctatatccca
      541 taaaacacaa cgtcaacaca aagtgtgttt ttcaaactaa attgtcatga tatttaaatt
      601 gcttcatgat acaattaata agatatacta ctcgtttaag attttcgatg ggaggtttca
      661 aatgatagag ttattatcca ttgcactcaa gcattctaat attattttaa attcaatatt
      721 tattggtgca tttattttaa acttattatt cgcctttacc attattttca tggaaagacg
      781 ttctgccaat tctatctggg cttggttact agtcttagtt ttcttgcctt tattcggctt
      841 cattttatac ttactattag gacgacaaat tcaacgtgac caaattttca aaattgataa
      901 ggaagataaa aaaggattag agttaatcgt tgatgagcaa ttagctgctt taaaaaatga
      961 aaacttttca aattccaatt atcaaattgt aaaatttaaa gaaatgattc aaatgttgtt
     1021 atataataac gcagcatttt taacaacaga caacgattta aaaatataca cagacggcca
     1081 agaaaaattt gatgacctaa tacaagacat ccgtaatgct actgattata ttcattttca
     1141 gtactatatt attcaaaatg atgaattagg tcgtaccatt ttaaatgaac ttggtaaaaa
     1201 agcggaacaa ggtgtagaag ttaaaattct ttatgatgac atgggttctc gtggactgcg
     1261 taaaaaaggc ttacgcccgt ttcgcaataa aggtggacat gctgaagcat ttttcccatc
     1321 aaaattacct ttaattaact tgcgtatgaa caatcgaaac catcgaaaaa ttgttgtaat
     1381 agatgggcaa attggatatg ttggtggttt taatgttggt gatgagtact taggtaaatc
     1441 aaaaaaattc ggctattggc gagatacgca tttacgaatt gtcggggatg cagtgaatgc
     1501 attgcaatta cgatttattc tagattggaa ttcacaagcc acacgtgacc acatctccta
     1561 tgatgatcgt tatttcccag atgtaaattc tggtggaaca attggcgttc aaatagcttc
     1621 tagtggtcct gacgaagaat gggaacagat taaatacggc tatttgaaaa tgatttcatc
     1681 tgctaaaaaa tcgatttata ttcaatctcc ctatttcata cctgatcaag cctttttaga
     1741 ttctattaaa attgcggcat taggtggtgt tgatgtcaat atcatgattc ctaataaacc
     1801 tgaccatccg tttgtttttt gggctacttt aaaaaatgca gcatccttat tagatgccgg
     1861 tgttaaagta tttcactacg acaatggctt tttacactca aaaacacttg ttatagatga
     1921 tgaaattgca agtgtgggaa cagctaatat ggaccatcgc agtttcacat tgaatttcga
     1981 agtcaacgct tttatttatg accaacaaat tgccaaaaaa ttaaaacaag cttttataga
     2041 tgatttagca gtatcttctg aattaacaaa agcacgttat gctaagcgaa gtctttggat
     2101 taaatttaaa gaaggtattt cacaattatt gtcacctatc ttataaaata gaaatatgag
     2161 gagtgtaact ttaatgcaac aatcagacgt cattagtgct gccaaaaaat atatggaatc
     2221 tattcatcaa aatgattata caggccatga tattgcgcat gtatatcgtg tcactgcttt
     2281 agctaaatca atcgctgaaa atgaaggtgt taatgatact ttagtcattg aactcgcatg
     2341 tttgcttcat gataccgttg acgaaaaagt tgtagatgct aacaaacaat atgttgaatt
     2401 gaagtcattt ttatcttctt tatcactatc aaccgaagat caagagcaca ttttatttat
     2461 tattaataat atgagctatc gcaatggcaa aaatgatcat gtcactttat ctttagaagg
     2521 tcaaattgtc agggatgcag atcgtcttga tgctataggc gctataggtg ttgcacgaac
     2581 atttcaattt gcaggacact ttggtgaacc tatgtggaca gaacatatgt cactagataa
     2641 gattaatgat gatttagttg aacagttgcc accatctgca attaaacatt tctttgaaaa
     2701 attacttaag ttagaatctt taatgcatac agatacggcg aagatgattg ctaaagaacg
     2761 tcacgacttt atgatgatgt acttgaaaca gttttttacg gaatggaatt gtcacgacta
     2821 gacattgaag ttgtagtatg atgatgcgat gtaatggcgt gttgttgtgg aagcttggtg
     2881 tcatgccatg ttactttgat gtgttgttgt gggagcttgg tgacatgtca tgctactttg
     2941 atgtgctggt accacgatgc gtcttgatgt agtgctatga tgtggcattg cggtgttatg
     3001 gtgttataga caggtttggc gttgatgcca tgttactttg atgtgctggt accacgatgc
     3061 gacttgatgt agtgctatga tgtggcattg cggtgttatg gtgttataga ccggtttgat
     3121 gttgatgcca tgttactttg atgtgctggt gctacgatgc gacttgatgt agtgctatga
     3181 tgtggcgttg cgctgttatg gtgttatagc caggtttggt gttgatgtca tgccgttacg
     3241 attctatgat atgttgttgg gacgttgcaa tgtgtattat gccgttgtga cgttattatt
     3301 tcacactgtt acatgtataa gtgaattgct gtggaaattt gcgacatata ctgctacact
     3361 gatgaatcat tgtgtcaaga tgacattgcg atgaagaatg acaactctgt tattaaccac
     3421 tttttacata ctgaaaactc gttaatatta tttcaaataa aaacagcagt aggatgactt
     3481 tcacatttga aatcatctta ctgctgtttc tatttatcac atattgtata atgtgacact
     3541 aagtttcgct attgaagcga aaaataatgt gcgccctata aagttaaaat tatcttcaac
     3601 ttttagggtg cacattattt ggacttgcta aggttatttc tttttctttt tagacacaac
     3661 ttgtgtgttt ttgccttttt tattgctgcc gccgttgtgc tctctttcat acgcttcaat
     3721 gaaaggttgt acttcttttt tagcgacttt ttcataataa atgttcgcaa agtgtgtttg
     3781 aactacaagg aacgccgcac tgactgacca gtacaaacca agtgctgatg ctgagcttaa
     3841 tgaaatccag ataatcataa ttggtgaaat gaccatcatc atgtaaccca tttgacgttg
     3901 ttcgtctggc atcgttttac ttgatacata tgcttggata aagtataaaa caccggcaat
     3961 aattgtaatc caaatatcag gacgtcctaa atcgaaccat aagaagtgtg gatatttaaa
     4021 caaaccatct acaagttggt ctttaagtac aaagtataat cccatgatga ttggtaattg
     4081 gattagcatt ggtaaacaac ccaacatact cttaatcggg ttcatgtcat actttttata
     4141 tacttgcatt aattcttggt ttgcagccat tttttcttct tgtgtacgcg cacgtttcac
     4201 tttttcttga attttttcaa cttctggctt tgcaactttc attttttgac gcatcatatg
     4261 actattttta tagtttgaca acatgaatgg taataaaata atacgaatta ccaatacaag
     4321 gataataata gctaaaccat aattgtcgtt taataagtta tttcccaacc aatccaatac
     4381 atttttcatt ggatctacga atgtattgta gaaaaaccca ctacgttttt caggtttaga
     4441 atagtcacaa ccagccaaaa agaccataat acctaaaaat aatggtagta acgctttttt
     4501 cttcattttt ccacctctat cattatattc acataggatt tattctatca cattaatgag
     4561 tacgtatgaa acaataagtg gaaaaattta actaattatt aaaaaaatct ttgaatcgat
     4621 taacagtctt ttcaatattt tcacttttag aaatggctga aatgactgaa attccattgg
     4681 cacctgcttc tacaatcggc gccacattat tagtattgat accgccaata gctacaatcg
     4741 gtagttgcgg attcatttct ttaaacgttg caatcatttc tggacctact ggtatatgcg
     4801 cgtcatgctt cgacggcgta ggatagattg gtccaacacc tatataatca acatgagtta
     4861 aatcagattt tgcatactca tctaaatcac taatactaag tccaataatt ttatcagtga
     4921 aatattgtgc tatctctttg actttcgcat catcttgacc gacatgtata ccatccgcgt
     4981 taatttcttt tgccaaggat acatcatcat taacgataaa aggcacatca tattgatgac
     5041 agagatgctg taattcttta gctaatacaa gtttatcgtt tccttttaaa gctgattcac
     5101 ccttttctcg aaattggaaa agtgtaatcc ctgcctttaa tgctgcttct aatacttcat
     5161 gaatagttcg atgactcgga acatcggagg tcccacaaat aaaatacaca tttagatacg
     5221 attggttaaa catattattc cacctcttga atacgaatcc gttgttgata ggttgtttca
     5281 tttaaatgat ataacgtatc aagcaacaat ggtgaaaacg taccaggacc accacaattt
     5341 tcattttcag cagctacctc agcagcaata ttaaatacgc ttaccgcttc aattaacgct
     5401 tctatgtctg gttctgtttc tctaaataaa aatccagcaa taacgcctcc taataaacaa
     5461 ccagctccag ttactcgtgc taataatgga gatccattag ctaatacgaa ggctttatta
     5521 tcttgaacaa taacgtcctc tttacctgtg attactattg cagttttata tgttgcgtaa
     5581 gcctttttcg ctattgcaac cgcatcaaga ttagcatcac tatctgtacc tttcatagtt
     5641 gctgtatcat caatcaacgc taatatttca gatgcattgc ctttaattac tgatactttc
     5701 gctgatttca ataataattt acaaaattgc tttcgatatg tagaagcacc aacagctaca
     5761 gggtcaaata caataggtaa gcctgcctca tttgccgttt gagcaatcgc aataatatct
     5821 tgttcatttt ctgctgttaa agtaccgata ttgattaata gcgcttgtgc aactttgtaa
     5881 aattcttcag cttcttcggg agcttcactc attgcagggc tagcaccaat acttaataaa
     5941 ccattcgctg taaaattttt aactacatcg tttgtataac aaattgttaa tgggttttca
     6001 atacgtattt tatttagata attcatcgtc taatccctct ttctttaaat atgcaaaatg
     6061 attcactgga cctctaccac ggccgatttc aggcgtatat tgtatactca ttgaaataaa
     6121 ctttttagcc ttgtgtacag cctcaaataa tggtctacct tttgcaagtt ctgccgttat
     6181 aactgctgaa aatgtacacc ctgttccatg cgtatgtttt gttttaaatc gttcattttc
     6241 aaatgtttga acaccttcgt tagtaaataa ataatctttt gctatatcag tatcatttga
     6301 atgaccgcct ttaatgatga cacctttact accaatctca ttaataaaga tgcggccagc
     6361 ctgcataatt ttttcttcac tatcaatggt tagtcccgtt atttcttcag cttctggtaa
     6421 attcggtgtt actacgtcag ctaatggcaa taacgtatgc tgcaagtttt gctttgtgtc
     6481 attatccatt aatgaatcac cgcttttcgc caacataaca ggatcaatta catatggaat
     6541 agattcatgt tgcattaaat aatgacgaat cgtttccata gtatctgctg tagcaatcat
     6601 ccccgtttta atagcatgag gtaaggtatc attgaagaca ctatcaagtt gttcatctac
     6661 ccattgatga tttaaattat gaatatgttg tacgcccaat gtattttgag caacaatact
     6721 tgtaacgacg cccataccat atacaccaca tgaatgaaat gattttaaat cagccattac
     6781 gccggcacca cctgttggat cagtacctgc aatggttaat gctattttag gtttaatcat
     6841 ttacttttcc tccaaattcc cattgttcta gtgtcatagc catattgaaa aatcgacgct
     6901 catgtatgca actttctaaa aatacttgct ttacttgttc caattccttg tcagacattg
     6961 attcggctaa tttattcatt aatgcctcaa atacgttaat tatgtcatcc atttcagtac
     7021 tataaaaatc aaaccatttt gccgtatctt tttctctatt aagcttatga tcactttgag
     7081 agcgcttagc taattctgca taaatatatg gacaaggtgc cattgcagca attgtataaa
     7141 tagcattttc acgactatgc gcttggaaat acatatgttt tatgtaatgg tcgccacttg
     7201 gaggccaaac ttttgtttta atgatttctt cgtatgattc accaacaatt tgcgctaaaa
     7261 tatcatgcgc aagtacttca ccttcaacca taaattctat ttgctctact aaaaatttta
     7321 cgtcattcat gctattcatt tttggaatta acaaagcata taagtttgta aattctttta
     7381 aatacgcagc atcagctttt aagtaatggc gtaatgcgtc agctcctata tctccggata
     7441 acatcttctg aataaagtca tcctcataaa tatcattaat gattggcttt gcagcttggt
     7501 acaatttttg tgaaaattcc attgtaaaaa atcctcccta aataaaaaaa actacttcca
     7561 acatgaaagt agtttgatgg caatgttgct atactagccc catcacttca ataactactt
     7621 tcctacgttg gtactaacca aatcaggtca taagggtctg aacaattcat ctcagccata
     7681 tcattaggct cccctagtag ttccttagta ttcaattgca aattaatctt agcaaacggt
     7741 ttcaacattt tcaattattg ttgctcagtt gtattattat ctttaaataa taattctata
     7801 atgacatata tttgcgaaat aaaaaaaccg gaacatatcg agaattcccc gatatattcc
     7861 aatctaaaag ttacttatat aactattaat tagctatgca taaatggctt atgcagtaac
     7921 ccaatgtcca gcgccaccag tgttatataa ttttactgct gcggcatctt gaacactttc
     7981 aggagcattt gctggtgata cacctttata tttagcaggt gctactgaat cccaagttga
     8041 ttgtaagaat tgatacttac cagctgcacc tgatgttgga tttacagcat gaatattgcc
     8101 acctgattca cgttgagcaa tttgttttag atgagcattc acatttactg atgaaccttc
     8161 tgatgatttt gattcagttg gtgttgcagt aacttgtgaa ttgtttgatg ttgatgcttg
     8221 tggttgttga gtttgagcat tttgtggtgc ttcaacttct tgtgattgta cttgattagc
     8281 ttgaacagct gatggtgcaa cattattagt tgcaggtgct tgtgcactca tgtctgctcc
     8341 attagtacct gttgcatggt aattccaagc aaagtgtgta ccatctgatt caaagtgata
     8401 agtaaaccct tcatagtcaa atgtataatt ataagcccca gcttcaattg gtttttgatt
     8461 taatgtttga tcatttgatt gcgccatttg cgctaaagat gctttattta agtccgcttc
     8521 acttgcatgg gcttcgtgac ctgcatttcc tgctacgatt cctaaaccta ctgctaatga
     8581 tgatgcgagt aatgttttct tcataatctt aaaatcctcc tacaagtaat ttgtttctct
     8641 aaaagtttta cagagactac tgtaacatct ataaaatccg tgcaggttac aacaaaataa
     8701 aaaaatagcc ttgtttttta cacagtgatt acaacgaaat cccccataat ttcagatatg
     8761 attctatcga gaattgtcat gacagtaaaa atgttttttt atttgcaatt ttttatgtaa
     8821 tcgttttgca atctatgtct attttctatt agaaaaacta cacaaaaaga cgacctacac
     8881 aacagtacta gtcatgtaag tcgttt
//
LOCUS       DAMQYT010000102         8904 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMQYT010000102 DAMQYT010000000
VERSION     DAMQYT010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.102"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            324..1619
                     /gene="purB"
                     /locus_tag="PGA33_001742"
     CDS             324..1619
                     /gene="purB"
                     /locus_tag="PGA33_001742"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDG6473549.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            1728..2030
                     /locus_tag="PGA33_001743"
     CDS             1728..2030
                     /locus_tag="PGA33_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473550.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            2202..2894
                     /locus_tag="PGA33_001744"
     CDS             2202..2894
                     /locus_tag="PGA33_001744"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HDG6473551.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKMAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            2891..5083
                     /gene="pcrA"
                     /locus_tag="PGA33_001745"
     CDS             2891..5083
                     /gene="pcrA"
                     /locus_tag="PGA33_001745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDG6473552.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            5087..7090
                     /gene="ligA"
                     /locus_tag="PGA33_001746"
     CDS             5087..7090
                     /gene="ligA"
                     /locus_tag="PGA33_001746"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDG6473553.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            7103..8302
                     /locus_tag="PGA33_001747"
     CDS             7103..8302
                     /locus_tag="PGA33_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HDG6473554.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGSTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(8391..>8904)
                     /locus_tag="PGA33_001748"
     CDS             complement(8391..>8904)
                     /locus_tag="PGA33_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="involved in the sodium dependent uptake of proline;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:proline symporter"
                     /protein_id="HDG6473555.1"
                     /translation="AAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGR
                     LSVLVVAIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGM
                     VSGALVVIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDL
                     NKVRDIVREK"
BASE COUNT         3360 a         1257 c         1693 g         2594 t
ORIGIN      
        1 aattgtaata aaaagttgga tagtgtagtt gaggggagaa aaactaaaat gcaaaaatat
       61 attctcaatg gatcattata aataccaatt agattaaagt attgcgaaca tttacaatta
      121 gaaatcagta ttaatctgcc atagataatt gtattaatgt aaaaaataaa atagaatgag
      181 aatatacgga aattttttat acatttataa ccaattctgt taaactgtga agagtagagt
      241 taaaaacgaa ctttttgttt atttttatta aaaaatgttc tgtttttgaa aattcgagta
      301 tacatacgat aggagttaaa aaaatgattg aacgctattc tagagaagaa atgtctaata
      361 tttggactga tcaaaatcgc tatgaagcat ggttagaagt ggaaatttta gcatgtgaag
      421 catggagtga attaggacat ataccgaaag ctgacgtaca aaaaattcgt caaaacgcaa
      481 aggtaaatgt cgaacgtgca caagaaattg aacaagaaac gcgccatgat gttgtagcct
      541 ttactagaca agtttctgaa acgctaggtg aagaacgtaa gtgggtacat tatggtttaa
      601 cttctactga tgttgtagat acagctttaa gtttcgttat taaacaagca aatgacatta
      661 ttgaaaaaga tttagaaaga tttatcgacg ttttagctga aaaagcaaaa aattataaat
      721 atacattgat gatgggacgt acacatggag tgcatgcaga accaactaca tttggtgtca
      781 aaatggcatt gtggtacacg gaaatgcaac gtaacttaca acgtttcaaa caagtaagag
      841 aagaaattga agtagggaaa atgagtggtg cagtaggtac ttttgctaac attcctcctg
      901 aaatagaaag ttatgtgtgt aaacatttag gtattggcac agcgcctgta tcaacgcaaa
      961 cattgcaacg cgatcgacat gcatactata ttgcgacatt agcattaatc gcgacatcac
     1021 tagaaaaatt tgccgttgaa atacgtaacc ttcaaaaaac cgaaacgaga gaagttgaag
     1081 aagcatttgc aaaaggccaa aaaggttcat ctgcaatgcc tcataaacga aatccaattg
     1141 gttctgaaaa tatcacaggt atttcaagag tcattcgtgg ttatattact acagcttatg
     1201 agaatgtgcc attatggcat gaaagagata tttctcattc ttctgctgag cgtattatgt
     1261 taccagatgt tacaatagca ctagattatg cattgaatcg tttcacaaat attgtggacc
     1321 gtttaactgt atttgaagat aatatgcgta ataacataga taaaacattt ggtctgattt
     1381 tctcacaacg tgtcttatta gcattaatta ataaaggtat ggttcgtgaa gaagcatacg
     1441 acaaagtaca accaaaagcg atgatatctt gggaaacgaa aacaccgttc cgtgaattaa
     1501 tcgaacaaga tgagtctatt acaagtgttt taacaaaaga agaattggat gaatgtttcg
     1561 atcctaaaca tcatttgaat caagttgaca ctattttcga acgcgctgga ttagcataaa
     1621 attaaataaa gtaacgattc agatttaata aaatataaat cgctacactt taatagatta
     1681 atgcgttaaa atgacataaa gaataaaatt ataggggttg tttcgatatg caaattgaaa
     1741 aattacgagg tgcagcgtta gatgaattgt ttgatgcaat actaacgtta gaaaatagag
     1801 aagaatgtta ccaatttttc gatgatttgt gtactgtaaa tgaaattcaa tcactgtctc
     1861 aaagattaca agttgctaaa atgattaagc aaggttatac ctatgcaacg attgaacaag
     1921 aatctggagc atcgactgca acgatttcta gagtgaagcg ttcattacaa tggggtaatg
     1981 atgcttatac aatgatttta gatcgtatga atattgaaac aaatgaataa taaatgaacg
     2041 ataaacaatg gttatctatc tgcactaata aggtagataa tcattgtttt ttcacgaaaa
     2101 aatttacaga gtaaaagaac ttaaatttca tattaagtct ttagaactcg acacttaaaa
     2161 atgctataat catatgtatg ttaaaaaagg agtttcggaa aatgtatgac attaaaaaat
     2221 ggcgccatat ttttaaatta gacccagcta aacatatttc agatgatgat ttagatgcga
     2281 tttgtatgtc tcaaacagat gcaattatga ttggtggaac tgatgacgtt actgaagata
     2341 atgtcattca tttaatgagc agagtaagaa gatacccatt gcctttagta cttgaaatat
     2401 caaacatcga aagtgtaatg cctggttttg atttttattt tgtacctaca gtactgaaca
     2461 gtacagatgt tgtatttcac aatggtacat tattagaagc gcttaaaaca tatggacata
     2521 gtatagattt tgaggaagta atatttgaag ggtatgtcgt gtgcaatgct gatagcaaaa
     2581 tggcaaaaca taccaaagca aatacagatt taacaacaga agatttagaa gcatatgccc
     2641 aaatggtcaa tcatatgtat cgattaccgg ttatgtatat agagtatagt ggcatttatg
     2701 gcgacgtatc aaaggttcaa gctgtctcag aacatctaac agaaacgcaa cttttttatg
     2761 gtggcggtat ttcctcagaa caacaagcga cagagatggc agctattgca gatacaatta
     2821 tcgtcggtga tattatttat aaagatatta aaaaagcttt aaaaacagta aaaataaagg
     2881 agtctagtaa atgaatgcgt tattaaatca tatgaataca gagcaaagtg aagctgtaaa
     2941 gacaacagaa ggaccattgt taattatggc aggtgctggt tcagggaaga cacgtgtttt
     3001 aacacataga attgcttatt tattagacga aaaagatgtc tcaccataca atgttttggc
     3061 tattactttt acaaataaag ctgcaagaga aatgaaagaa cgtgttcaaa aattagtagg
     3121 tgatcaagca gaagttattt ggatgtcaac attccactca atgtgtgttc gtattttacg
     3181 tcgtgatgca gatcgaattg gtatagaacg caattttacg ataattgatc ctacagacca
     3241 aaaatctgtt attaaagacg tcttaaaaaa tgaaaatatt gatagtaaaa agtttgaacc
     3301 tcgtatgttt atcggtgcga tcagtaattt gaaaaatgaa cttaaaacac ctgcagatgc
     3361 tcaaaaagaa gccacagatt atcactcgca aatggtagca acggtttata gtggatatca
     3421 acgccaattg tcacgtaatg aagcgttaga ttttgatgac cttattatga caacgattaa
     3481 cttatttgag cgtgtaccag aagttctaga atattatcag aacaaattcc aatatattca
     3541 tgtagatgag tatcaagata ctaataaagc acaatacaca ttagttaaat tattagcaag
     3601 taagtttaaa aacttatgtg ttgtaggtga ctcagatcag tcaatttatg gttggcgtgg
     3661 tgctgatatt caaaatatct tatcatttga aaaagactat ccagaagcga atacaatctt
     3721 tttagagcaa aattatcgtt cgacgaaaac gattttaaat gcggctaacg aagtgattaa
     3781 aaataattct gaacgtaagc caaaaggact gtggactgca aatacgaatg gtgagaaaat
     3841 tcattactat gaagcaatga cggaacgtga tgaagcggaa tttgtaatac gagaaattat
     3901 gaagcatcaa cgtaatggta agaaatatca agatatggca attttatata gaacgaatgc
     3961 acaatcacgt gtacttgagg aaacattcat gaaatctaat atgccataca caatggttgg
     4021 tggccaaaag ttctatgacc gtaaagaaat caaagattta ttaagttatt tgcgtataat
     4081 tgccaatagt aatgatgaca ttagtttgca acgtattatt aatgttccaa aaagaggtgt
     4141 aggtccttca tctgttgaaa aagttcaaaa ctatgcactt caaaacaata tcagtatgtt
     4201 tgatgcactt ggagaagctg attttatcgg attgtcaaaa aaggtgacac aagagtgtct
     4261 taatttttac gagttaatcc aaagcttgat aaaagaacaa gaatttttag aaattcatga
     4321 aatagttgat gaagtattac aaaagtctgg ctatcgagaa atgcttgaac gtgaaaatac
     4381 attagaatct cgaagtagat tagaaaacat cgatgaattt atgtcagtac caaaagacta
     4441 tgaggaaaat accccattag aagaacagtc attaattaac tttttaacgg atttatcgtt
     4501 agtagctgat attgatgagg cagatactga aaatggcgta acactaatga cgatgcactc
     4561 ggctaagggc cttgaatttc caattgtctt tataatgggg atggaagaat ctttattccc
     4621 acatattaga gcgattaaga gtgaagatga tcatgaaatg caagaagaac gtcgtatttg
     4681 ttatgtagca attacaaggg ctgaagaggt gttatatatc actcatgcga catcaagaat
     4741 gttatttggt cgccctcagt caaatatgcc atccagattt ttaaaggaaa ttccagaatc
     4801 actattagaa aatcattcaa gtggcaaacg acaaacgata caacctaagg caaaaccttt
     4861 tgctaaacgc ggatttagtc aacgaacaac gtcaacgaaa aaacaagtat tgtcatctga
     4921 ttggaatgta ggtgacaaag tgatgcataa agcctgggga gaaggcatgg tgagtaatgt
     4981 aaacgagaaa aatggctcaa tcgaactaga tattatcttt aaatcacaag ggccaaaacg
     5041 tttgttagcg caatttgcac caattgaaaa aaaggaggat taagggatgg ctgatttatc
     5101 gtctcgtgtg aacgagttac atgatttatt aaatcaatac agttatgaat actatgtaga
     5161 ggataatcca tctgtaccag atagtgaata tgacaaatta cttcatgaac tgattaaaat
     5221 agaagaggag catcctgagt ataagactgt agattctcca acagttagag ttggcggtga
     5281 agcccaagcc tctttcaata aagtcaacca tgacacgcca atgttaagtt tagggaatgc
     5341 atttaatgag gatgatttga gaaaattcga ccaacgcata cgtgaacaaa ttggcaacgt
     5401 tgaatatatg tgcgaattaa aaattgatgg cttagcagta tcattgaaat atgttgatgg
     5461 atacttcgtt caaggtttaa cacgtggtga tggaacaaca ggtgaagata ttaccgaaaa
     5521 tttaaaaaca attcatgcga tacctttgaa aatgaaagaa ccattaaatg tagaagttcg
     5581 tggtgaagca tatatgccga gacgttcatt tttacgatta aatgaagaaa aagaaaaaaa
     5641 tgatgagcag ttatttgcaa atccaagaaa cgctgctgcg ggatcattaa gacagttaga
     5701 ttctaaatta acggcaaaac gaaagctaag cgtatttata tatagtgtca atgatttcac
     5761 tgatttcaat gcgcgttcgc aaagtgaagc attagatgag ttagataaat taggttttac
     5821 aacgaataaa aatagagcgc gtgtaaataa tatcgatggt gttttagagt atattgaaaa
     5881 atggacaagc caaagagagt cattacctta tgatattgat gggattgtta ttaaggttaa
     5941 tgatttagat caacaggatg agatgggatt cacacaaaaa tctcctagat gggccattgc
     6001 ttataaattt ccagctgagg aagtagtaac taaattatta gatattgaat taagtattgg
     6061 acgaacaggt gtagtcacac ctactgctat tttagaacca gtaaaagtag ctggtacaac
     6121 tgtatcaaga gcatctttgc acaatgagga tttaattcat gacagagata ttcgaattgg
     6181 tgatagtgtt gtagtgaaaa aagcaggtga catcatacct gaagttgtac gtagtattcc
     6241 agaacgtaga cctgaggatg ctgtcacata tcatatgcca acccattgtc caagttgtgg
     6301 acatgaatta gtacgtattg aaggcgaagt agcacttcgt tgcattaatc caaaatgcca
     6361 agcacaactt gttgaaggat tgattcactt tgtatcaaga caagccatga atattgatgg
     6421 tttaggcact aaaattattc aacagcttta tcaaagcgaa ttaattaaag atgttgctga
     6481 tattttctat ttaacagaag aagatttatt acctttagac agaatggggc agaaaaaagt
     6541 tgataattta ttagctgcca ttcaacaagc taaggacaac tctttagaaa atttattatt
     6601 tggtctaggt attaggcatt taggtgttaa agcgagccaa gtgttagcag aaaaatatga
     6661 aacgatagat cgattactaa cggtaactga agcggaatta gtagaaattc atgatatagg
     6721 tgataaagta gcacaatctg tagttactta tttagaaaat gaagatattc gtgctttaat
     6781 tcaaaaatta aaagataaac atgttaatat gatttataaa ggtatcaaaa catcagatat
     6841 tgaaggacat cctgaattta gtggtaaaac gatagtactg actggtaagc tacatcaaat
     6901 gacacgcaat gaagcatcta aatggcttgc atcacaaggt gctaaagtta caagtagcgt
     6961 tactaaaaat acagatgtcg ttattgctgg tgaagatgca ggttcaaaat taacaaaagc
     7021 acaaagttta ggtattgaaa tttggacaga gcaacaattt gtagataagc aaaatgaatt
     7081 aaatagttag aggggtatgt cgatgaagcg tacattagta ttattgatta cagctatctt
     7141 tatactcgct gcttgtggta accataagga tgaccaggct ggaaaagata atcaaaaaca
     7201 taacaatagt tcaaatcaag taaaagaaat tgcaacggat aaaaatgtac aaggtgataa
     7261 ctatcgtaca ttgttaccat ttaaagaaag ccaggcaaga ggacttttac aagataacat
     7321 ggcaaatagt tataatggcg gcgactttga agatggttta ttgaacttaa gtaaagaagt
     7381 atttccaaca gataaatatt tgtatcaaga tggtcaattt ttggacaaga aaacaattaa
     7441 tgcctattta aatcctaagt atacaaaacg tgaaatcgat aaaatgtctg aaaaagataa
     7501 aaaagacaag aaagcgaatg aaaatttagg acttaatcca tcacacgaag gtgaaacaga
     7561 tcctgaaaag attgcagaaa aatcaccagc ctatttatct aacattttag agcaagattt
     7621 ttatggtggt ggagatacaa aaggtaagaa tattaaaggt atgacgattg gtttagctat
     7681 gaatagtgtt tattactata aaaaagaaaa agatggatcg acttttagta aaaaactaga
     7741 tgatagcgaa gttaaaaagc aaggtaaaca aatggctagt gagatattat caaggttacg
     7801 tgaaaatgat gatttaaaag atataccaat tcattttgca atttataagc aatcaagtga
     7861 agattcaatc acaccaggtg aatttatcac tcaagcgact gcagaaaaga gtcaaacaaa
     7921 gcttaatgaa tggcataata tcaatgaaaa atcagcttta ttaccttctt caacagcagc
     7981 agattatgat gaaaatttaa ataataattt caagcaattt aatgataatt tgcaatcata
     8041 tttttctaat ttcacacaag cagtagggaa agttaaattt gttgataaaa agccacaacg
     8101 attagtagta gatttaccaa tcgattacta tggacaagct gaaacaattg gtattacaca
     8161 gtacgttact gaacaagcga ataaatattt cgataaaatc gataactatg aaattcgtat
     8221 taaagatggt aaccaaccac gtgctttaat tagtaagaca aaagatgaca aagaaccgca
     8281 agttcatatt tacagtaatt aattgtaaat taaagataat aaaaaggtac caatgttagt
     8341 cataattttg attaacatta gtaccttttt aatttgttaa gacttatgaa ttatttttct
     8401 ctaacgatgt cacgaacttt gtttaagtca gtttcaacaa atgcaccagg ttttttagta
     8461 agtttactta caacatatgt aacgattaca cttacaataa atccaggaat aatttcatat
     8521 aagccgaata tttcgttgat atgtgccaat ggtttaatcc acgcaatcca aacgataacg
     8581 actaaggcac ctgaaaccat tccacttaca gcaccggcac gtgtcaaacc tttccagtaa
     8641 agtgcaaata gcacaagtgg actgaacgat gcaccaaatc cggcccaagc gttacctact
     8701 aagtttagaa ttgtgtcgtt tggattccat gcaatcgcga tggcaacaat tgctacaact
     8761 aatacagata atcttccaat cataacaaat tctttttggt gcgttttagc tttttcttca
     8821 ccacgaatta atttataaaa gtcttccgtt agtgaactag atgttacaag taattgtgaa
     8881 gaaatcgtgc tcataattgc agct
//
LOCUS       DAMQYT010000103         8782 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMQYT010000103 DAMQYT010000000
VERSION     DAMQYT010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8782
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.103"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..303
                     /gene="deoC"
                     /locus_tag="PGA33_001749"
     CDS             <1..303
                     /gene="deoC"
                     /locus_tag="PGA33_001749"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDG6473556.1"
                     /translation="VKVIIETVLLDHDEIVKASELTKTAGADFVKTSTGFAGGGATAE
                     DVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADSDY"
     gene            331..1509
                     /gene="deoB"
                     /locus_tag="PGA33_001750"
     CDS             331..1509
                     /gene="deoB"
                     /locus_tag="PGA33_001750"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDG6473557.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
     gene            complement(1640..2455)
                     /gene="phnE"
                     /locus_tag="PGA33_001751"
     CDS             complement(1640..2455)
                     /gene="phnE"
                     /locus_tag="PGA33_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDG6473558.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(2452..3252)
                     /gene="phnE"
                     /locus_tag="PGA33_001752"
     CDS             complement(2452..3252)
                     /gene="phnE"
                     /locus_tag="PGA33_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDG6473559.1"
                     /translation="MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            complement(3254..4027)
                     /gene="phnC"
                     /locus_tag="PGA33_001753"
     CDS             complement(3254..4027)
                     /gene="phnC"
                     /locus_tag="PGA33_001753"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDG6473560.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            complement(4241..5197)
                     /locus_tag="PGA33_001754"
     CDS             complement(4241..5197)
                     /locus_tag="PGA33_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDG6473561.1"
                     /translation="MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            5426..6970
                     /locus_tag="PGA33_001755"
     CDS             5426..6970
                     /locus_tag="PGA33_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDG6473562.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVKYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSL
                     KASSLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQNEKVTFDIPQNVSVNEPFEMTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNVTIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            7021..8556
                     /locus_tag="PGA33_001756"
     CDS             7021..8556
                     /locus_tag="PGA33_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDG6473563.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVKNDEVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            8631..8782
                     /locus_tag="PGA33_001757"
                     /pseudo
     CDS             8631..8782
                     /locus_tag="PGA33_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc peptidase"
BASE COUNT         2944 a         1430 c         1635 g         2773 t
ORIGIN      
        1 gtaaaagtga ttattgagac ggtattgttg gaccatgacg aaattgtaaa agcgagtgaa
       61 ttaacaaaaa cggctggtgc ggacttcgtt aaaacttcaa caggttttgc aggtggcggt
      121 gcgactgcag aagacgttaa attaatgaaa gatacagtag gtgctgatgt agaagtaaaa
      181 gcatcaggtg gcgtacgtaa tttagaagat ttcaataaaa tggttgaagc aggtgcgaca
      241 cgtattggtg cgagcgcagg tgttcaaatt atgcaaggtt tagaagcaga ttcagattac
      301 taatatatat aaattttggg agtgatagct atgacaagac catttaatcg tgtacattta
      361 atcgtaatgg attcagtagg tattggtgaa gcgccagacg cagctgattt taaagatgaa
      421 ggttcacata ctttaagaca taccttagaa ggtttcgatc aaactttacc aaaccttgaa
      481 aagttaggtc tagggaacat cgataaatta ccagtagtaa atgcagttga acaaccagaa
      541 gcatactata ctaaattgag tgaagcttca gttggtaaag atacaatgac tggtcactgg
      601 gaaattatgg gattaaatat tatgcaacct tttaaagtat accctaatgg attccctgaa
      661 gagttaattc aacaaattga agaaatgaca ggtcgtaaag ttgttgctaa caaaccggca
      721 tcgggtacgc aaattatcga tgagtggggc gagcaccaaa tgaaaactgg tgacttaatt
      781 gtttatacaa gtgcagaccc agtattgcaa attgctgcac atgaagacat tatcccatta
      841 gaagagttat atgatatttg tgaaaaggtt cgtgagttga caaaagaccc taaatattta
      901 attggtcgta ttatcgcacg tccatatgtt ggtgaaccag gaaactttac acgtacatct
      961 aatcgacatg actatgcgtt aaaacctttt ggtaaaactg tcttagatca tttgaaagac
     1021 ggtggttatg atgttattgc catcggtaaa attaatgaca tttatgatgg tgaaggtgta
     1081 acagaagcgg ttcgtacgaa gagtaacatg gacggtatgg atcaattgat gaaaattgtt
     1141 aagaaagatt tcacaggtat tagcttctta aacttagtag actttgatgc attatacggt
     1201 catcgtcgtg ataaaccagg ttatgcacaa gcaattaaag atttcgatga tcgcttgcca
     1261 gaactgttta gcaacttaaa agaagacgat ttagtaatta ttacagcaga ccatggtaat
     1321 gacccgacag cgccaggtac ggaccatacg agagaatata tcccagtaat tatgtacagt
     1381 ccgaaattta aaggtggtca tgcactagaa agtgatacta cattcagttc tatcggtgca
     1441 actatagcag ataatttcaa cgtaacatta ccagagttcg gtaaaagtta tttaaaggaa
     1501 ttgaaataga ataaatttag atattataaa aacagcagtg aagttaacta taacaatagt
     1561 tttcttcact gctgttttta ttataataga gaaacgtaag acggtaggac ttcttattta
     1621 ggagtatcct gatttaatgt taaacaatac gttttcggat tgaaccggaa attaaatcga
     1681 caattgcgac cattagtact aaaccgatta atataatacc tacacggtcc caagaacgtg
     1741 tttgaatggc aaatatgagt ggtgtcccga taccaccagc cccaattagc cccagtatag
     1801 aagctgaacg taagtttagt tcaaagcgat aaagtatgag tgatagaaag gcaggcataa
     1861 tttgtggtat gactgcaaat acgagtgttt taatcttatt cgcaccactg gcctttaatg
     1921 attctacagc actgaaatct agaccttcaa tatcttcagc taaaagtttc ccaagcatac
     1981 ctacggaatg gatacctaaa gctaatacac ctgaaaatga acctgggcca acagctttga
     2041 taaatataag tgccattaca atttctggga agacacgtat aacacttaaa ataaatttgc
     2101 taacacctga aactgggcgt agctttacca tattatttgc acctagaaat gctaatggaa
     2161 tacagataat tgcggcgatg aaagtaccta caacggctat cgcaaaggtt tcaagtaaac
     2221 cacgtaataa gtcttcgcca tctggtatat agatatagct gatatcagga tggaataatc
     2281 cgctgaatat ggattttaag atttctaatg atttactttt aagttctaaa cttggtacac
     2341 ctgcaaatgc ccagatgata atagctaaga cgacaattgc aataagccat cttttaatca
     2401 attttcgttt gtgtgctttt gtgtgaacat tatattttgc tatttcctgt gtcatgcgag
     2461 atgtgccctc actttcgtac tgatgtaatc aatgacgacg acgataacta aagtaaataa
     2521 aataatcgtt gctgtttttg gatattgaaa taaaccaagt gtttgatcat aaaacaatcc
     2581 aataccgcca gcgccgacta atccaagcac agctgaagca cgtatattta cttcaaatgc
     2641 atataatacg tatgacataa atgacgatat ggcttgtggt acaacaccga aaacaatcca
     2701 ttttatttta ttagcgccaa cagccgtcat tgcttccatt ggacctggat ctatcgtttc
     2761 caatgattca tataataatt ttccaataat acagatagtt aaaataaaca gtgctaatat
     2821 ccctggaatt tgaccgattc caaatacagc cacaaagatt gctgctaata acaaatctgg
     2881 aatagtacga actatattta aaataaagcg cgagggtatt gaaatccact tttgatgaac
     2941 gatattgcta gcacataata acgcaattgg tattgaaacg atgctaccta atactgtact
     3001 tacgatagcc attcgaatgg tatctaacat tggcgttgta atttgttgta aatactcgaa
     3061 atcaggtgga atcatttgtt tgaatagatc acctatttga ggtattccta tcattaaatc
     3121 tccaaaatta aatcccgtat aaatgaagct ccaaatgata agcacaatga ttaacatgaa
     3181 ggtaaaactc gtttttaaag aaaccttttt ctttaaaagg gagtcatact ttgtaggtat
     3241 ttctaaaggc atgttagttc actcctagct tttcatcttc tttaattgta cgtccatata
     3301 tttcactaaa tacgtcatct gttgcttcag atgcaggacc atcatagaca acttcaccat
     3361 cacgtaaacc aatgatgcgt gtgccatatt cttttgccaa gtcaacaaaa tgtaaattaa
     3421 ttaaaattgt gatgcctaat tcttggttga tttttcttaa atcatccata acctgtttcg
     3481 tagttaatgg gtctaatgaa gcaactggtt catctgcaag aataatttca gattcttggc
     3541 atagcgcacg tgcaatagat atacgttgtt gttggccacc tgataattca tcagagcgtt
     3601 gattatattt atctaagata ttgacgcgtt ctagtgcatc cattgcctta attttgtctt
     3661 cttttgggaa taaacctaat accattttcc aagtagggtg ataacctaca cgtccactta
     3721 gtacatttcg taatacactt gaccgtttaa ctaaattaaa atgttggaaa atcataccta
     3781 tatttcggcg catttctaat aatgctttac catgggcttt agtgattgat ttaccttgga
     3841 tgaaaatttc acctgacgtg atatcatgca aacgatttac agatcttaat aacgtggatt
     3901 tcccagcacc agatagtccg acaataactg caaattcacc tttttcaata tttaagttaa
     3961 tatttttcaa gcctacatga ccgttaggat agactttact gacgttttta aattcgattt
     4021 gactcatgtt tgacaccttt ctttaataaa gaaaagccaa ggtaaactga aatatcatag
     4081 acattgggtt tctgtgtaca aatggtttca aatgtacagc gacacctttc tatatctgtt
     4141 tcagtttagc gcctagcttt tcaatattaa tctagaatat atctattgaa cgaaagcttt
     4201 taataccaaa ttcgctaatg attcatttgt taaataatga ttatttcata tctttaacta
     4261 atttttcgta ctctcttaca atgtcgaaat ttgaatcttt cgtttctgtg tatccttcat
     4321 gtgaataaac ttcgctaata attttgtgac cttcttttga tttagcaatg tctataaaag
     4381 cttttttcaa tttttcttga aaatctttat ccatatctgg tcttacagaa attgtgtcat
     4441 tcggaatagc ttgtgttaat tttaaaattc gtgtgtcttt aaatacattt ggttggtctt
     4501 ttttcacagt attacgtgca tcgttaaata cagccgcagc atctacatct ccatttaata
     4561 atgagataac tgcttggtca tgacctttaa cattcacaat tttcatatct ttagttgcat
     4621 taatacctgc ttcgtttttt aacatcgcaa gtgggaatgt atatccagca gttgatgtta
     4681 catcttgtaa ggcaattttc ttacctttta aatctttcaa gcttttaatt tttgagtctt
     4741 ttttaacaag aatttctgat ttataactat ctacaagttc tttacttgct gaaccatctt
     4801 cttttacacc gaaacgttgt gcttgtaata ataaatcagc tgctttttga tcatgtgcta
     4861 atgtgtatgc cgttggtggt aagaaaccaa catcaacttt tttagacttc atagcttcaa
     4921 caattgtatt gtagttagtt gatacagaca ctttaactgg aatccctaat tctttagata
     4981 gtaatttttc taatggtttt gctttagctt ctaatgttcc agcattttgc gaaggtacaa
     5041 attgaacggt taattcttta ggtttgtatc ctcctgattt agaatccgaa tcattactag
     5101 cgttcttttg attatctaaa gaacttgagt ttccacatgc tgctgcaaaa acaatgactg
     5161 ctaacattaa tacaaataaa cacttaaaat ttttcatttg ataactgtcc ccttcacatt
     5221 cttaatcgtg taatatgtat gcaatgaaat tacacttata gcgtaccaca tttaaatgtt
     5281 aaaaagtatt ttataaaaat gaattttaat aactttcttt aagagtttga taagattttg
     5341 taaatttttc gttaaattgc tcatatgcaa cgaattttta tcctataatt caagaacaaa
     5401 tttattttaa aaggagcctc acaaaatgaa aaaaatatat aagtcattaa ctgtctctgc
     5461 aattgttgca acggtatcat taagtgcttt accgcaatct ttagctataa cgcatgaatc
     5521 gcaacctaca aagcaacagc gaacggtatt attcgatcgt tctcatggtc aaacagctgg
     5581 tgctgcagat tgggttagtg atggtgcatt ttcagattat gcggattcaa tacaaaaaca
     5641 aggttatgac gttaaagcta ttgatggtca ttcgaacata acagaagcaa gtttgaaaag
     5701 ttccaaaata tttgtaattc ctgaggctaa cattcctttc aaagaatcag aacaggcagc
     5761 aattgttaaa tatgtgaaac aaggtggcaa tgttgtcttt atttcagatc attacaatgc
     5821 tgaccgaaat ttaaatcgta ttgattcatc ggaggcaatg aatggttatc gacgtggagc
     5881 atatgaagat atgtcgaaag gtatgaatgc agaagaaaaa agttctactg caatgcaagg
     5941 tgtgaaaagt tcagattggt tatctacaaa ctttggcgta cgttttcgat ataatgcact
     6001 aggtgattta aatacgagca atattgtttc ttcaaaagaa agtttcggta ttactgaagg
     6061 tgtgaaatct gtctctatgc atgccggatc gacattagca attactaatc cagagaaagc
     6121 aaaaggtatt gtgtatacac cagaacaatt gccagcgaaa agtaaatggt cacatgctgt
     6181 agatcaaggt atttataatg ggggcggtaa agcagaaggc ccctatgtag caatttctaa
     6241 agttggaaaa ggtaaagcag catttatcgg tgattcatca cttgtggaag atagttcgcc
     6301 caaatatgta agagaagata atggagaaaa gaagaaaaca tatgatggtt ttaaagaaca
     6361 agacaacggt aagctattaa ataatataac ggcttggatg tctaaagata atgatgggaa
     6421 atcacttaag gcgagtagcc taacattaga tacaaagact aagttgcttg attttgaacg
     6481 accagagcgt tcaactgagc ctgaaaaaga gccatggtca caaccgccga gtggttataa
     6541 atggtatgat ccaacaacat ttaaagcagg tagttatggc agcgaaaaag gcgcagatcc
     6601 tcagccaaac acaccagatg atcatacacc accaaatcag aacgaaaaag taacatttga
     6661 tatcccgcaa aatgtttctg taaatgagcc atttgaaatg acaatacatt taaaaggatt
     6721 tgaagcaaat caaacacttg aaaatcttag agttggtatt tacaaagaag gcggacgtca
     6781 aatcggacaa ttttcaagta aagataacga ttataaccca ccaggttaca gtactttgcc
     6841 aacagttaaa gcagatgaaa acggaaatgt cacaattaag gtcaatgcta aagtacttga
     6901 aagtatggaa ggttcaaaga ttcgtttaaa actcggtgac aaaaccttga ttacaacaga
     6961 cttcaaataa atatataata gaaataagaa agatgtttgt gattgaagga gtgagtgagg
     7021 atgtcaaaca tagcatttta tgtcgtgagt gacgtacatg gttatatttt cccaacagat
     7081 tttacgagta gaaatcaata tcaacctatg ggattgttac tagcgaatca tgttatagaa
     7141 caagacagaa ggcagtatga ccaaagtttt aaaatagata atggtgattt tttgcaaggg
     7201 tcaccatttt gtaattactt aatcgcgcat agcggcagta gccagccttt agttgatttt
     7261 tataatcgaa tggcattcga ctttggtacg cttggtaatc atgaatttaa ttatggatta
     7321 ccatacttaa aagacacttt acgcagactc aattatccag ttttgtgcgc taatatatat
     7381 gaaaatgata gtacattgac tgataacggt gtgaagtatt ttcaggttgg agatcaaact
     7441 gttggtgtga taggtttaac gacacaattt attccccatt gggaacaacc agagcatatt
     7501 cagtcactta cgtttcatag tgcttttgaa atacttcaac aatacttacc tgaaatgaag
     7561 cgacatgcag atatcattgt ggtttgttac catggtggat ttgaaaagga tttagaaagt
     7621 ggtacgccga ccgaagtatt aacgggtgaa aatgaaggat atgccatgtt agaagcgttt
     7681 tctaaagata tagatatctt tattacgggt caccaacatc gacaaattgc tgaaaggttt
     7741 aagcaaacgg ctgtgattca acctggtacg agaggtacaa ctgtaggcag agtagtcttg
     7801 agtactgatg aatatgaaaa tctatccgtt gaatcatgtg aattacttcc tgttatagat
     7861 gattccacat ttactattga tgaagatgac caacatttac gaaagcagtt agaggactgg
     7921 ttagattacg aaattactac attgccatat gatatgacga ttaatcatgc atttgaggca
     7981 cgtgtggcac cgcatccttt tacaaatttt atgaattacg ctttattaga aaaaagtgac
     8041 gcagatgttg cctgtacagc tttgtttgat tctgctagtg gtttcaagca agtcgtgacg
     8101 atgcgagatg ttattaacaa ttacccattt ccaaatacat ttaaagtttt agctgtaagt
     8161 ggtgccaaac ttaaagaagc cattgaacga tcagcagaat attttgacgt gaaaaatgat
     8221 gaagttagtg tgagcgcaga cttccttgaa cccaaaccac aacactttaa ttatgatata
     8281 tatggtggcg taagttatac cattcatgtt ggaagaccaa agggacaacg tgtgagcaat
     8341 atgatgattc aaggtcacgc agttgattta aagcagacat atacaatttg tgtaaataat
     8401 tatcgtgcag taggcggtgg tcagtatgat atgtatatcg acgcgccagt tgtaaaagat
     8461 attcaagttg aaggcgcaca attacttatt gattttttat caaataataa tttgatgcgc
     8521 atcccgcaag ttgttgattt taaagttgaa aagtgacgga tatatgtaaa acttacttga
     8581 ttttaatcgg gtaagttttt gtttttgttt aaagcgagaa agtattatct ttagacgacg
     8641 tgattgaaat gtcacgtcct gtcaaaatga atagtttatt aaagttgtat tttgatagaa
     8701 atttactgac accacaaaaa ttattgaatt atttaaaagt tgatgaaaca tttttgaatc
     8761 atctagcagg tattaattta aa
//
LOCUS       DAMQYT010000104         8742 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMQYT010000104 DAMQYT010000000
VERSION     DAMQYT010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8742
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.104"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(108..1622)
                     /locus_tag="PGA33_001758"
     CDS             complement(108..1622)
                     /locus_tag="PGA33_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDG6473564.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            complement(1615..2592)
                     /locus_tag="PGA33_001759"
     CDS             complement(1615..2592)
                     /locus_tag="PGA33_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6473565.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQLLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            complement(2814..4595)
                     /gene="recQ"
                     /locus_tag="PGA33_001760"
     CDS             complement(2814..4595)
                     /gene="recQ"
                     /locus_tag="PGA33_001760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDG6473566.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(4607..6490)
                     /locus_tag="PGA33_001761"
     CDS             complement(4607..6490)
                     /locus_tag="PGA33_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDG6473567.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LIEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREENEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            complement(6761..8701)
                     /gene="ltaS"
                     /locus_tag="PGA33_001762"
     CDS             complement(6761..8701)
                     /gene="ltaS"
                     /locus_tag="PGA33_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HDG6473568.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
BASE COUNT         2670 a         1588 c         1195 g         3289 t
ORIGIN      
        1 tttttaatca ttattgcatg tttaaattga acataattat agatttaaat ataacaacgc
       61 cacataagat ataatttggt gttacgcatc ttatgtggcg ttggtcgtta tttgattaac
      121 ccttttgctt ttaaataatc tttcgcaact gtatatgggt cttcattttt aactgttact
      181 ttatagttca tcatttgcat atcttcatca gatattttgt tttctagttt gtttaacggt
      241 ttcttaattt ctggatgttt ctttaaaaag ctttctttaa ataatggtgc tccttgatat
      301 ggtggaaata cgtgcttatc atcttttaac acaaccatat catattgttt taattcagcg
      361 tcagttgaat atgcatctat taaattaata ttacctttat tgatcgcttg atatctcaac
      421 ttaggttcca ttgtacgtat gttatctaaa tttaaattat aagccttttg aacagctttg
      481 taaccatctg gacgatcatt aaattccaat gtaaatcctg gtttaagttg atctttaacc
      541 ttatttaaat caccaattgt acgtatatta tgttgtttag caaaatcacg ttttacagct
      601 aaagcatacg tattgttata cttcattggt tttaacatag tcatttgata tttcttttca
      661 agactttgct tagcttgttc ataaactttt ttctcttctt ttgacttcaa tggttctttt
      721 gttaattcac ctaaaactgt tccagtaaat tctaaatacc catctatatc gtcagatttt
      781 aaagcattaa ataaaaatgc tgttttgccc ataccatctt tcacttctac agtatttttg
      841 gtctcttctt ctattaaaat tttatacata tttgtaataa tcgatggctc ggagccaagc
      901 tttccagcta acgtaatttt atcacctttt tgtgcaaaca taggaatagc gatagccagt
      961 ataataatca tcactataac acctaacgtc atcaataact ttttataaga taatttagcc
     1021 ataaatctta aaattaaatc aaatataatt gccaataagg ctgctggaat tgcacctaat
     1081 aatatcaacg atgcattgtt acggtctata cctaataaaa ttaaatctcc tagtccgcct
     1141 gcaccaatta atgctgctag tgttgctgta cctataatta ataccatagc cgttcttaca
     1201 ccagccatta taacaggcat tgctatcgga agttcgactt tagttaaacg tctaaatggt
     1261 ttcataccta tacctttagc cgcttcaatg agtgatggat caacttcttt aattccagta
     1321 tacgtattcc ttaaaattgg taacaacgca tacactacaa gtgcaataat tgctggcaca
     1381 cgaccgatac caaataaagg aatcattaaa cctaataatg ccaacgatgg tatggtttga
     1441 agaattgccg caatattcat tacgatttca gatatcgttt tagtcttcgt taataaaata
     1501 cctaatggta ccgcaatagc agttgcaatc aataatgcga taaatgatat ttgaatatgt
     1561 tctatcattg tcgaaaagag ttgcccctta cgttcactca atatgtcgaa aaagttagtc
     1621 atgttgagct acctcctttt tctgggacaa atatttgaag atatctttcc tatcaataac
     1681 atattgacct acgctatctt cttgcatgac aatgacacgc tcgctctctg ataaaagttg
     1741 atacaatact tcaattggtt gattgtcata aacaattgga taagcgctca tagatgtaac
     1801 ctcatcgatt ggtttcataa tatccaagtc acggataatt gcgttctctt caacacatgg
     1861 cgcatcatct tctaaatgac tacccataaa ttgtttaaca aattcacttt gaggattatt
     1921 tttaaatcct tctggtgtgt caatttgttc aatatgccct tcattcaaaa gacaaatctt
     1981 atcaccaagt ttcatcgcct cttgaatatc atgtgtaaca aatatgattg tcttcttaat
     2041 tttagtttgt aattcaatta aatcatcttg aagtttttct cggctgattg ggtctaatgc
     2101 actaaacggt tcatccatta aaataactgg tggatcagct gctaacgcac gtataactcc
     2161 tacacgttgt cgttgccccc ctgacaattc atcaggtttt ctgtttttat atttttcagg
     2221 ttctaatcca accatttcaa gtaattcatc tactctttta tctatatctt tttctttcca
     2281 ctttttcatt tgtggcactt gtgcaatatt ttctttgatt gtcatatgtg ggaataatgc
     2341 aatctgctgc aatacgtatc caatatccca acgcatttcg tatactggat aatcacttat
     2401 tggtttatct ttaaaataaa tataaccttc acttaagtga atgagtcgat taatcatttt
     2461 taatgtcgta gtttttccac aacctgaagg tccaattagc acaaaaaatt caccctcatt
     2521 aatattgaaa ctaatgttat cgacagcaac atgtttgcca taacgcttag ttacattttt
     2581 aaacttaatc actttgccac ctcttttttt ctcatagcat aaaaccgaga ttatatgtat
     2641 gtattcccta tttaaccacg tttattacaa ttttcaaatt taaatgattt atccttgaac
     2701 ttttttaata aaataatgaa taataggtaa tctccagtta agaaatagtg ttattttacc
     2761 ttgaattcaa aaaaacaccc agtaaaacaa ggaatgctta ctaggtgtct tcactatact
     2821 ttggctttat aattttgaat cgtttctaaa aatgctggac aataatgttt taatttgtaa
     2881 ctacctacgc catcaatatt aatcatatct tgtttcgaag caggcttacg tttagcaaat
     2941 tcctccaacg tgtaatcaga aaatatactt acaggtgcta tcgttaattt gtcacttaac
     3001 tttttacgaa cttctaccaa ctgactgaat aatactcggt caaccccttc aaccgtattt
     3061 ataaatactt tttcagtcgc tttttgctta aatggtgttg tgaatacttc tacttcatta
     3121 ctgagtaatt ttttaattga agtatcacac attaatattt cgtcattttc atttaagaac
     3181 cctttgaatc ttaattcatc tattaagtga cttaattctg atgttgtgta acctttcatt
     3241 aaaccatggg ttgaaatttg gtcataacct ttatacttaa tataatctgt tgactctcct
     3301 cttaacactt gaatgataac actataactc tcttgttgtt tcatacgagc gatgcaacta
     3361 ataatcatct tagcttcttg tgtcatatta tatgatttat cttgttgaac acaattacta
     3421 cattgttcac attcttctaa tttttcattc ggttcaaaat aatggacaat tgttgcttct
     3481 agacattttt ttgtttttgt atattgaatc attttagtta acttttcgcc cattttatct
     3541 ttatagtcat catcagcttg agagactgtt ataaaatact cgtgtaaatt gatatcgcgt
     3601 tcgctaaata acaaaataca ttcacttttc aacccgtcac gacctgcacg acccgcttct
     3661 tgataataag attctaaatc tccaggcata ttataatgaa taacaaagcg tacattggat
     3721 ttatcaatac ccataccaaa agcatttgta gcaacgacta ctttaacacg atcaaataag
     3781 aaatcattct gcgcttcttc tctttcttta ttgctcaaac ctgcatgata tataacactt
     3841 tcaattttct gactttctaa ggcttcttga agctcttcaa cttgcttacg tgtagaacaa
     3901 taaataatac ctgcatcttc atcgtgtgtt ttaatataat ccaatataaa tttttgacgt
     3961 tgataagtag gatttacttt aaaaattaag tttctacgct tagtactcgt tttaatttga
     4021 tcagtttgag cgatatttaa cttttctcta atatcttgct gtacttcaac cgtggcagtt
     4081 gctgtcaacg ctattattgt aaaatcttga ggtaacgtaa atacttttga aataacattt
     4141 tggtaactcg gcctgaaatc atgaccccat ttagaaatac aatgcgcttc atcaaacgcg
     4201 actaagtgaa tctttatacg ctgaagcata tttaaaaaat atcggttttc aaatcgttct
     4261 ggtgcaacat acaaaaattg aatttctcca tttgataatg ctttttcaat acgttgttgc
     4321 tctttttgag tcaaactact atttaaaaaa gcagcttgaa ttcccatcgc ttttaattga
     4381 tccacttgat ctttcattaa tgatattagt ggacttatta caattgttgt accacctaac
     4441 aataaacctg gtacttgata gcatatagac ttacctccac cagttggtaa gacaccaagc
     4501 acattacgat ggtctaatac tttgctaata atttcttctt gtcctggtcg aaacgtttca
     4561 tacccaaagt aatgcgataa tgtttgttgc atcataaatt gaccccttat tgttgttctt
     4621 taatttcttc taactcactc catcttgtga tgtctaaatc atattgaatt tcaagttgtt
     4681 ctttttcttc gtttaattct ttaattttcc cataatctgc acttgcctca atcatgagca
     4741 catcaatttc ttccattctt acttccgctt gttctatgcg tttcatcaat tgttcatatt
     4801 ctaatttttc tttatatgat aaaccatttt tcttacgtac agttgtagaa gatttagatt
     4861 gttgcttcaa tgtggattta tttttatcta atgatttttt ataactttca taatcttcaa
     4921 aagttccgat aatcttttcc atctgaccat catgaataaa ccaatatgac tgtgcaactt
     4981 tatttaagaa gtagcgatca tggcttacgg taatcactgt accaccgaaa gtatgaatat
     5041 aatcttcaag tattgttaaa gtctcagtat ctaaatcatt tgtcggttca tctaacaaca
     5101 gaacatttgg ctggtgtacg agtagacgta ataaatacaa acgcttttgc tctccaccag
     5161 ataatttata aactttttta ccatgagttg cacttggaaa taaaaatcgt tcaagaagtt
     5221 gtgtaataga taccgaggtt ccatcttttt cttttgcgat ctcattttct tctcttaaat
     5281 aatcaatcat acgaatatct ctatccaggg tctcatctgt ttgcttaaaa taagctactt
     5341 taaccgtctg cccagtcttc aatttacctt cgaattgttg gtcttctcca ctcaaaatat
     5401 ttaagagtgt tgttttacca gctccatttg gcccaacaac accaatacgc tcaccttttt
     5461 gaataatttc cgtcagatgt tcaattaata ctttatcatt aatagccttt gataagtctt
     5521 ctaattcgaa cacttgctta cctaatcttg aataagcaag attcaattca cctttatcgt
     5581 ctttatattg ctggttaact tcattttcta ggtcattaaa tcgattaatt ctagcttgtt
     5641 gctttgtagt acgagcctta gctccagccc tcatccaagc aagttcttcc ttatataaag
     5701 ctcgttgctt ttgttgttgc ttttgaagtg ttacttctct ttcagcgcgc atttcaatat
     5761 aagattcata gttaccagga tatgacgcta acttacctct gtttagttca ataattctag
     5821 tggaaacttc atttaaaaaa tatcgatcat gggttacgaa taaaacagta tgaggatatt
     5881 gcttcacata attgatcaac cagctgattg attcgaagtc taaatggttc gtaggttcat
     5941 ctaataacaa taaatctggt tgttctatta atgttttagc aagtacaaca cgtttttgtt
     6001 gtccgccgga taattcttta atgtatttag tagtatcatg tatacctagt tttgagagga
     6061 ttgttttaat ttcagcgtta tagtcccaag catcatgttg atccatagca tcttgcgcat
     6121 ccatcattcg cttgaacaat ttatcacttt ggtcatccgc ataagcttgt actgcctgct
     6181 catattgctt gatgatgcgt aaagttgttg tatcagaact taatactgca tcaaaaactg
     6241 tcatatcttc attaaggtcc tgtttctgag acgaatatcg aattcgatat tgatttggat
     6301 gcataacatt ggctgtaaaa tcatcatcaa taccaccaat tacttttaac aacgtacttt
     6361 tccctgtacc atttatgcct actaaaccta ttttttcacc ttctgaaatt gataaatcta
     6421 ggttatcgaa tatagtctta tcggcataag atttatttaa atgttcaatt ttatatgctt
     6481 ccatacccat cttccttctt ctgttgaaat ctctacgtac ttaaatcagt tctatacatt
     6541 atacaccttt ttgaaatcaa aagaaatcga taccgttaag tgtcataatt taatatattg
     6601 ttgcttcaac tataaataaa acttgttaag gctttgatga ctatgctaat gatctttata
     6661 atataccttt ttatcaacac atataatgta aaacataata taaaagaaca taataaaaac
     6721 ccggattgat attttatcaa tccgagttcg tgtttagata ttatttttta gagtttgctt
     6781 taggtcctgt ttcatattta tacttcgaag gatttacctt tttgaagtct ggatttttgt
     6841 agaatctaaa caaatcacca ttaagcacgt tgtcactcat ttcgagatcc ttttcaactt
     6901 gctttttatt cttttcgaaa tcagctggtt gagtagttat gagttcatta tttttattag
     6961 aataaatctt accattaaca tatttataat cttttgttat aaagtcacca tttctgaatg
     7021 gaactacttg attatgacct ttagagaata aatcagtacc gaacattaaa tagttcttcg
     7081 tatctatacc agccaaatgt aaaattgttg gcattacatc gacttgacca gcatattcat
     7141 tattgatacc accagattta ccagggattt taatccagaa accagttctg tttaaatctg
     7201 taaatttagc cggtgtgatt ttttcaccta atagtttttc catggcattg ttatggtttt
     7261 cagagatacc atagtggtca ccataaatca taatcactga attgtcatat aatccttttt
     7321 tcttcaagtc attaatatat tcttctaatg cttcgtctaa ataacgtgct gtttgaatat
     7381 aaccatcaac tgttgcatca cctgtgtttg atttctcaat agttgcatcc ttttcatcta
     7441 atgtgaatgg atagtggtta gtcaatgtaa ttaaatgaga atagaatggt gatttcatct
     7501 tagcttgata attagcagaa tctttaaaga aaattttgtc tttcaagcct aagtttacaa
     7561 cgtttttatc tgacatgtca tagtatgttg catcatagaa tttatcgata ccaaagtgtt
     7621 tatatacttg gtctctgttc cagaatgttt tatagtcacc gtgcatgaca tcagatttgt
     7681 agccttgctt ttgatctaaa attgctggta atgactgata cgtattatct ccttttaatg
     7741 aaaaggcaga accttgcggt aaaccgtata aactgttatc cattgtaaat tcagagtcag
     7801 atgttttacc ttgacctgtt tgatggaaaa agttagggaa gtatgtgaat tgctctttcc
     7861 cacttgataa tttgtttaaa aacggtgtta cttctttacc attaaccttt ttattaatta
     7921 agaaggtttg gaaactttct aaatgaatct taataatatt tttcttcttt gccaccccat
     7981 aatattctgg gttaggctct gtttgacgtt gtttcgtata atttaatact tttgttaagt
     8041 catcttcaga tgctagcgct ttttgttgat tattttcgat agttttaaca ccatcgtata
     8101 ctgtaaagtt ataaggtcct aaatatttca ctaaatattt atggtcaaat gtacgtgtta
     8161 ataattctgg tctgtcagtt tcagcaaaag ctaagtttaa gaagaataat gctactgaag
     8221 ccgccattac gacaggaaca aatttcttac taaatgcttt tgtgtctaac cattttgttt
     8281 taaatattaa aatgaataag taaactaacg tatcaatgaa ataaacaaag tcataccatt
     8341 tgaatgatgc actaaccgca ccacccatag attctacgtt acctacttgg tttaaagtac
     8401 taaacgttaa aaaatcagag aagaatctaa agtacacaac attggcatat aataggaacg
     8461 tcaataagaa gccgcctatg aacatgaacc aaaatgcttt tttgccttta aagaatagga
     8521 acacacttaa aaccagtgct actaaactat aaggattcat caataatatt aagttttgta
     8581 ctaaaccttt aacacctaaa gaaaaatcaa cataataaga aaaatacgtc ttcaaggtaa
     8641 tcgttattac ggttaataag aagaacgcaa aaagactaat tttctttttt tgtgaactca
     8701 tgattctttc ccccgttatt tagataataa atcttgcgaa ag
//
LOCUS       DAMQYT010000105         8728 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMQYT010000105 DAMQYT010000000
VERSION     DAMQYT010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8728
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.105"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            12..1535
                     /locus_tag="PGA33_001763"
     CDS             12..1535
                     /locus_tag="PGA33_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473569.1"
                     /translation="MSKKLKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFNNAQAQQSDYSKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKD
                     FEKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYNDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            1603..2769
                     /locus_tag="PGA33_001764"
     CDS             1603..2769
                     /locus_tag="PGA33_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6473570.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRTLITWFYTMLWSIVGVVWIVFSIPNIFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            2766..3686
                     /locus_tag="PGA33_001765"
     CDS             2766..3686
                     /locus_tag="PGA33_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6473571.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            3700..4359
                     /locus_tag="PGA33_001766"
     CDS             3700..4359
                     /locus_tag="PGA33_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6473572.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWTYTY
                     Y"
     gene            complement(4411..5361)
                     /gene="lytM"
                     /locus_tag="PGA33_001767"
     CDS             complement(4411..5361)
                     /gene="lytM"
                     /locus_tag="PGA33_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HDG6473573.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(5687..6679)
                     /locus_tag="PGA33_001768"
     CDS             complement(5687..6679)
                     /locus_tag="PGA33_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDG6473574.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWKSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHTVEVSFKEGE
                     VVIKDNPIGVLTNHPDLNWHYSNLRQYINISPYPATANLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNELVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            6913..8289
                     /locus_tag="PGA33_001769"
     CDS             6913..8289
                     /locus_tag="PGA33_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDG6473575.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAS
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSIICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
     gene            complement(8366..8716)
                     /locus_tag="PGA33_001770"
                     /pseudo
     CDS             complement(8366..8716)
                     /locus_tag="PGA33_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         2925 a         1478 c         1416 g         2909 t
ORIGIN      
        1 gaggaagcaa aatgtctaaa aagttaaaaa ttataattcc tattattatt gtcttactat
       61 taataggtgg aatcgcatgg ggagtttatg cattttttgc taatacaccg aaaaatacat
      121 acctaaaaag tgaacaacaa acagcaaaaa tgtataaaga ttattttaat gaccgttttg
      181 aaaatgaagt gaagttccaa gaaaagatga aagataattc gtttttatct tcattagaat
      241 taagcgcaga tgcatcagat gaaattgtta aagggcttgg cattcctaaa tctgttgtta
      301 atgcatcgaa aattaaaatg tcatatggac atgatcctaa aaaagagaaa tcaatgatta
      361 atcttgaacc aacaatcgca gactctgcat tagggaaatt ccagttagct gcagataaag
      421 ataagcatta tttcgaatca ccattattta aagggaaata tagtgttaat aattctgatt
      481 tattatcaac ttattcaaaa cttacaggtg aagatgaaga aacagcaaaa gaaaatggta
      541 ttacaaacca acaactaaat ttaaatactc tttttaataa tgctcaagca caacaaagtg
      601 actacagcaa aattgccgaa aaatattccg aacttattgt cgacaaatta gatgacgata
      661 attttgataa aggtaaaaaa gaagaaatta aggttaatgg tgaaaagtac aaagttagac
      721 ctgtcacgtt aacacttagc agagctgaca ctaaaaaaat tacattagct gtattagaag
      781 aagctaaaaa ggataaagac cttaaaaaat taatggaaga acaaggtgct acaaaagact
      841 ttgaaaaaga catcaaaaaa gcaattgacg atgtcaaaga aactaaaaag gatgaatttg
      901 ctaaaattca atctaaaatt tataccgaaa aacatacgat tgtaaaacga gaaattacta
      961 ttacagacaa agaaaataat aaaactaaaa tcaaaggtac taatacttta gaagacgata
     1021 agttaaaact agattacgca cttgatttcg atcaagataa atacacgtat gctgaagcga
     1081 aatatacaat taaaggcgta tcttctaaag aaaaagataa taaatataat gataaatacg
     1141 aatttggtaa aaagacagaa tatgatgaat caaaaatcaa attagataac caagaaaaag
     1201 tagatggcac aaaacgtcaa gataaaggta aaatcactgt cgcattagat aaatatagcg
     1261 acgaaaatga attcactttt gaaaataata tagattctga cgtaaaaaat aacactcaga
     1321 aatctacgtt aaatatcggc atcaaatatg ctgaagaacc aattaatttc attttaaaat
     1381 ctagcacaaa attgaaagca gatattgatt ttgatgatag tggtgcaaaa gatttcaata
     1441 gtctatcttc aaaagaccgt gaaaaacttg aaaaagaaat cgaaaaaaat ggcggcaaaa
     1501 tgtttgaatc aattttaaaa aaggcatcta aataactaaa atataaaaat aagattggaa
     1561 ggtcatcttg cctttcaatc ttatcattgg aggagtggtt aaatgaaaac aatacatttg
     1621 tttcgcatct accactcttt tttattgaaa aagtggtatt tgattatcta tttactattt
     1681 atattagcgg cactccttat tacattaacg acaatccaac atgtaacaga agatgacaat
     1741 cattttaata taggtgtcgt agataaagat caatcaagtg aaacgaaatt aatcttaaac
     1801 tctattggta aagggagtaa cctaggaaaa aacgtgagca ttaaagcata tgatgataag
     1861 caagcacata ctttgttaaa aaaacataaa cttcaaggct attttgtttt tgataaaggt
     1921 atgaccaagg cattttataa acaaggcgaa ctaccaattt cagtatatac atatgatcaa
     1981 caatccatga aaagtgtcgt gctatctcag ctaacagatt ctgtttacca acgtcttatg
     2041 cgatcaatgg gtggcatctt agcttttcaa gacttagcac cgaaagcatc acattctgac
     2101 agtatcaatg ttatgactga tttgctgatt acaggattaa accgttcagg tgcatttaac
     2161 ttagaaccga ttcatttata cgatacgggc agttattatg caattacagg atttttaaca
     2221 acggtattca tctttgcact atctttattt acagttttga aaatgaatca agatactgta
     2281 ttgaaagcgc gattgaaaat gtttcatttt tctaaagagc gtttattaat cattcgtacg
     2341 ttgattacat ggttttatac tatgttatgg agtatcgttg gtgtagtttg gattgtgttc
     2401 agtattccga atatctttga attatataat tggccaacgt tagccattca tttaagctat
     2461 tatgttacct ttttaatact gtggttatta ctcatcgaac ttttaacaac aggcttgttg
     2521 aatagtatta gtaaagtcat attagctatt gtcatcctgg tgttatctgg tttaacaata
     2581 cctacgattt ttttacaaca tatagcaaat ggtgttttca atattcaacc gttcgcagtt
     2641 gtaacaaatc aattgttaga aattatttta aacaactaca ttttagaact gcaccctagt
     2701 ttctatctta gcttcatcgc acttttgata attaatttag ctgtattggt ttggaggtat
     2761 cgtcaatgaa gccttatata caacttgttg tgttcaagca atggttacaa tacatcttgc
     2821 tcgtaacaac cattgtcatc gcactcgtac ttattggtat cggttaccgt gtagcacatg
     2881 acaacttcaa aataccgatt accattcaag atttagacca aaccactgca tcaaaatcat
     2941 tcgtcaataa aattaaacaa tctgactatg taactattaa aaaagtcgat gaagatgaaa
     3001 gctatattga agatgatgtt actaaaaagg aagctatttt aagtatgcaa attcctaaag
     3061 gtttctctca aaaattaaaa gagaaccgtt taaaagaaac gatacagtta tatggtagag
     3121 atgactttat aggtggtatt gctgtagaaa ttgttagtag ttcattatat gagcagcaaa
     3181 ttcctaacat tatttatgaa caccttgagg atatgaaaca gcatcaatcc atcgatgcta
     3241 tcaacaagtc ctatcataaa catacacctg aatctaaaat caaatttgtg tcgcttacta
     3301 aacaagcaca acactctatt tcaattagct taatctttgc ggtgatttta tttgttagcg
     3361 ctgttcaagt agtacttcat tatcgtttaa accaacaagc agcattgcaa cgattatcac
     3421 aatatcattt aagccgtttc aaactatata gtacttatgt aatgacacat acgattttgt
     3481 tattgttggt actattggca gttagtctat atttgtctca accactcagc ttaatatttt
     3541 acttaaaatc actgttactt atattgattt atgagatagg tatcgtcttt atcttattcc
     3601 atattcaaac aataagtcat cgattattca tgacatttat atatgcactt gctatgggta
     3661 tcgtatactt gattattttc atgtaaagga gcgtaactga tgatagaaat taataacctt
     3721 tcaaagcgtt accgtaacaa acagattttc aatcatttaa ctatgtcctt tgatagtaat
     3781 cgtttaaccg tattacttgg tgataatggt gctggaaaat caacattact tcgtatgatt
     3841 gctggtattg aaaaagctaa tgatggaact atcaactatt tcggcgaaaa atggaatcaa
     3901 agacaaatac aaaatcacat cggttatgtg ccacaagaca ttgcgttatt tgaacacatg
     3961 acagtggctg aaaacattaa attttttaaa tcactttgta aaaatccaat taacgataca
     4021 actatcaacg aatatttaca gcaattaaac tttgatgata cgtctgccaa agtatctaca
     4081 ttgtccggtg ggaataaacg taaaattaat atattagtag gtttactagg tcaacctcga
     4141 attctcattt tagatgaacc gacagttggt attgatttaa aatctagaca tgacatccac
     4201 caactactta acatcatgaa atctaaatgt ttaattatat taactaccca tcatttagat
     4261 gaagttgaag cacttgcaga tgatatcaag ttaattggcc aagatccttt ttatcaacat
     4321 gttttagagg acaaacaatg gacttatacc tattattaaa acgaaaaaat cccaagctgc
     4381 gtatgatatc gcaacttggg attttctgta ttatctactt tgcaagtatg acgttgggtc
     4441 tactgcatat tgattaccga tgccaccaga catacgttgg aagtgtacgt gaggcgctgt
     4501 tgaattaccc gtactacctg aatatgcaat ttggtcacca gctttgactt tatcaccagc
     4561 tgaaacagtt aaacgattat tatgcatata ccattggtag ttattactgt tcgcttcttt
     4621 aatcgttact tgattgccgc caccatagtt actccaacct gcttgtacta ctgtaccatc
     4681 agttaatgag taaactggtg aattttcagg cattgcatag tcgacaccgt aatgcgcacc
     4741 accaccgtga tattgtccat atggttgtag ttgtttacga cttgttaacc agcttgcgtc
     4801 tttcgcatga ccactagctg tcgctttact tgctgatcca ccattttgat tagatgtgcc
     4861 attaggataa ttgacctttc cattaccatc atggctgtta tacgcttggt tgttgttact
     4921 atgtgaataa tagctcgcgt ctggacctac atttgattga taaccatatt gattaatatg
     4981 ctgttggctt tgactcgctg tgtagtcatt gttatctcct gctgttgctg gattcacata
     5041 tgtttggccg cttccattgg catttgcatt ttttggataa cagttataaa aataatgcgt
     5101 atgtccttga gcatctacga atgtatagct atattcttta ttatcaaaca ttgcttgatt
     5161 ccagttacca tcaggtgtgt gatgataatc cccattagaa tcaattgtat aataagtacc
     5221 ataagatacg tcttgtgatt gtgttgacat ttgtgtatgt gcttgttggg tgtttgtcgt
     5281 ttctgctgca tctgcttgat gcgccattgt aaatgtagcg aagcccatcg ttgcaatcgc
     5341 tgctgctgtt aattttttca tgtataaaac atcctccatt aaagttaaag ttagttttca
     5401 attaaactgt actgcacata ctaaaagaat tagacaactg agtaaaggat ttaattctca
     5461 ttttccaact atttaatatt cccgaaatgt tttactaaac tcattacatt gtcattacaa
     5521 aatagccata cattgatatt aaaatgacat ctctcactgc attcgtttaa ccttttataa
     5581 attttcaaaa ttaacaacta atcgttcgtc catgttcgcg attcaacgct aatgcatgat
     5641 agtaatcatc catcaaatca tatcaaccaa attccattat caatcgctat tgattgtcat
     5701 tcaactttct aatagtgata tgcttctcag gcttaaaaat cgtcatatct tttctattaa
     5761 ttaaatcatc tgtgagcttt aatgctacta attcattgct gccataatac ttaatatata
     5821 acgttcttgt agttaaattt attacggtct gatacatcgt atagtgattg tcagcatcat
     5881 gcggacgtac aattccaatc ggtatattta ccgcatctaa taaataaaat gcattcatta
     5941 aatccatttc tttatcattg ttttgagcaa tgtttgcttt cataaatgcc attctcacaa
     6001 agcgctcagt tgaagtaaat ccacctggca atccaaatgt acctgcttca ttgcctaaag
     6061 gttcaatcgt tacaccttcc aataaatttg ctgttgctgg ataaggagaa atattgatat
     6121 attgtcttaa attactataa tgccaattta agtctggatg atttgttaag acaccaatag
     6181 gattatcttt tataaccact tcaccctctt taaatgaaac ttcgactgta tgtccagttg
     6241 catcggaaac atgataatgc aatggcggaa cttcaccgat gtcatttaaa tatacagcta
     6301 caacatgtat ttgggatgct tgttgtttca tatcttcaat gcttgttgta tatcccaaaa
     6361 tccatgtcac aatttcattt tgcgtaatat tcatcgcgtc cgctttgtgt gttgatccat
     6421 atgaactata acctcggaaa tattgtgttg aaatggcaac gccatgttca ttaacaccat
     6481 caccataaat aaaaccttcc atatctgttc ctgtgccaat aaagccatat tgcgtttggc
     6541 ctgtcgtgcc agtgcgagat ttccaacgat aatttctagg cgtcactgct ggcgaaccat
     6601 ctaatggata atcataatcc atcgtgcgtc caagaagtac ttgattattt aaagtttgta
     6661 ttgtgaatcc tgtgcacatt gttctcactc ctctgtacct tcatttactt taatcacttt
     6721 caaataaagc tgtttcactt aaacatacta taaaaaatca attatacaag caattaattg
     6781 atattcattc tcaataactg tggtatgata tgtaaggaaa tcatgactta tgtgtgagtg
     6841 aacgatcatc tatacatccg ttcacttcat ctcatgactt tctatattta atttttacaa
     6901 ggagtgacat ctgtgaataa cacacaatct tcaccacgca gtaatattat tattgcgatt
     6961 atgttgtctg cattaacata ttggttgttt gcacaatcat ttattaatat aggacctctc
     7021 gttggtcaaa cgtatcaaac ctctcctgcc gtgttaaatt tatctattag tttaacttcc
     7081 ttcgccacag gtatcttcat ggtggctgca ggtgatattg ctgataaaat aggacaactg
     7141 agaatgacat acatgggtct cataatcagt atgtttgcat ctcttctatt aattatatcg
     7201 gacatcactg cactgctcat catcggtaga attttacaag gtctatcagc agctatcttg
     7261 ttaccttcaa cagttggcgt gttaaataat caatttaaag gagaacattt aagacgagcg
     7321 attagttatc taatgattag tactgttggt ggcatcggcc tagctggtgt tatcggcggt
     7381 ttaattgcct caaatttcgg atggcaaacg aatttcatca ttagtatagt cattgctttc
     7441 attgccatat tgcttctaaa aggcacacct gaaaaagtaa gtcaacatag ccaccgtcat
     7501 ccattcgatt acaaaggtat gtcgattttc gctgttatga ttggtagctt tacattattg
     7561 ttaacacaag gattcgaaca aggttggttt agtacatttt caatcatttg tctgagcatt
     7621 tttatcatca ctacgttgat attcatcatc atcgaacgtc gacatgaagt accttttatt
     7681 gatttctcag tattacgcaa ccgtccgttc attggtgcat ttttaaataa ctttgtttta
     7741 aatagcggtc taggcgtaac agtggtcttt ttcatatatg ctcaaacaca ccttggttta
     7801 tcagctgcgc aatctggact tgttacattg ccatatgcca ttgtggcagt tgcgatgatt
     7861 cgtttaggtg aaaaagcaac attacgtttc ggtggcaaat tgatgctcat cattggtccg
     7921 ttgttccctg tcatcggcat cactattatt agcatgactc aattattggc atcacaatat
     7981 gtcattgcag ttatcattgg tttcgtcata tgtgcgatag gtaatggttt agtcgcaaca
     8041 cctggactta cgattgcaat tttcagtatg cctaatgaaa aagttggttt agctacagga
     8101 ttatataaaa tgagtggtac attaggtggc gcctttggta tagcactaag tactacagtt
     8161 ttcagtatgt tacaactaaa ctatgcacca agtgtagctg caaccgtaac atttatagtc
     8221 agcattgtat tgatgatcct tggctcattg tctgcataca tgatcattcc aaaaacagtt
     8281 aaatcttaaa tataatagaa gaattatgtt tcgaaatatc tttatcactt taaaatgata
     8341 tacaagaaat ccaagaaaaa taagcgaact gaataaataa agattcaatt aacgcatcag
     8401 tattaggatt cactctaaaa cgattaatag ttttataaga aggtgtttga tcttgagcta
     8461 accacatcat tcgaatactg tcatgaagta atttctctat tctacgacca gaaaatacag
     8521 attgagtata tgcatataag atgattttta acatcatctt tggatgatag gatgttgcgc
     8581 cacgatgatg tctgaattca tcgaatttgc tatcaggtat cgtttcaaca atttcattaa
     8641 catgtcgcga aatatcattt tgaggaattc taacagaagt ttttattggt agtgtaagtt
     8701 gggcaaagtg tcttattttt ttaaagta
//
LOCUS       DAMQYT010000106         8692 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMQYT010000106 DAMQYT010000000
VERSION     DAMQYT010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8692
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.106"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            82..201
                     /locus_tag="PGA33_001771"
     CDS             82..201
                     /locus_tag="PGA33_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473576.1"
                     /translation="MTIFTQLSDRIKKAISKINQANNIPNNARKSPMYHSPIT"
     regulatory      284..418
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            480..1025
                     /locus_tag="PGA33_001772"
     CDS             480..1025
                     /locus_tag="PGA33_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDG6473577.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     gene            complement(1131..1379)
                     /locus_tag="PGA33_001773"
     CDS             complement(1131..1379)
                     /locus_tag="PGA33_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDG6473578.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            1487..2440
                     /locus_tag="PGA33_001774"
     CDS             1487..2440
                     /locus_tag="PGA33_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDG6473579.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTKLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVELEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            2430..3809
                     /locus_tag="PGA33_001775"
     CDS             2430..3809
                     /locus_tag="PGA33_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDG6473580.1"
                     /translation="MLHDILRNKFGFESFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQAVLTTLQSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            3962..5422
                     /gene="ebpS"
                     /locus_tag="PGA33_001776"
     CDS             3962..5422
                     /gene="ebpS"
                     /locus_tag="PGA33_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HDG6473581.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHDTIKNAENNTEHSTVSDKSEA
                     EQSQQPKPYFTTGANQSETSKNEHDNDSVKQDQDEPKEHHNGKKAAAIGAGTAGVAGA
                     AGAMAASKAKKHSNDAQNKSNSGKANNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            5547..5663
                     /locus_tag="PGA33_001777"
     CDS             5547..5663
                     /locus_tag="PGA33_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473582.1"
                     /translation="MLFNKVFSRILAYQFNFLNSHQKDKTPQSTNLTRKNKK"
     gene            5824..6810
                     /locus_tag="PGA33_001778"
     CDS             5824..6810
                     /locus_tag="PGA33_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HDG6473583.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(6925..7893)
                     /locus_tag="PGA33_001779"
     CDS             complement(6925..7893)
                     /locus_tag="PGA33_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDG6473584.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            7970..8629
                     /locus_tag="PGA33_001780"
     CDS             7970..8629
                     /locus_tag="PGA33_001780"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDG6473585.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
BASE COUNT         3214 a         1377 c         1479 g         2622 t
ORIGIN      
        1 tgatttaaat aataatataa atggaagtac tgatataaaa ctttttaacc tactagattc
       61 ttataatttg ctttccattt tatgacgatt tttactcaat tgagtgatag aatcaaaaaa
      121 gccatctcaa aaattaatca agcaaacaac attccaaaca atgctcgcaa atcaccaatg
      181 tatcactctc caattacgta actatgattt aatttaagca tagttattga ggttttgtga
      241 tatatagtat aaaattaatg agaattaaat ttaataatgt aaaattcatc ttcggggtcg
      301 ggtgtaattc ccaaccggca gtaaataaag cctgcgacct gctagtatgt atcatattag
      361 tggctgatct agtgagattc tagagccgac agtatagtct ggatgggaga agatggaggt
      421 tttttgttgt gcaataatcc tcctattctt acgagatgaa tggaaggaga aaattgaata
      481 tgcaacaaaa taaacgtctt atcacaataa gtatgttgag cgcgattgcg tttgtgttaa
      541 cttttatcaa gtttcctata ccatttttgc caccatactt aactttagat tttagtgatg
      601 taccgtcact actagctaca tttacgtttg gaccagttgc cggtatcgta gttgcactgg
      661 ttaaaaattt attgaactac ttatttagta tgggcgatcc agttggacca tttgctaact
      721 ttttagcagg cgcaagtttc ttattaactg cttacgccat ctataaaaat aaacgttcaa
      781 caaaatcttt gattactgga ttaatcattg caacaatcgt tatgactatc gtgttgagta
      841 ttttgaacta tttcgttcta ttacctttgt acggtatgat atttaactta gctgatatcg
      901 caaataatct taaagtaatc attgtttcag gaattatacc attcaatatt attaaaggta
      961 tcgttatttc tattgtattt attttactat atagaaggct tgcgaatttc ttgaaaagaa
     1021 tttaatcaaa ttaaagcaaa ataatataca cataataata aaaagcaggt gactatcaat
     1081 aaacgatagc ttgcctgctt tttctataga acatttgtct aaaaaatcaa ttattcaaat
     1141 tttaaagcgt ccccatcaaa tgattcgtct gcaattttaa tagaatctgt agggcatcca
     1201 tcaattgcat cttccatatc ttcatataat tcctcaggta cttctgcagt accttggtta
     1261 tcgtcaagga ttacgaaagc aataccttcg tcgtcgtaat catatatatc tggcgctgct
     1321 gcaccgcatg caccacatgc aatacaagta tccatatcaa cgattgtata ttttgccaat
     1381 gtcttcgcct cctttgataa aaatgctaaa atagtaatgt gactaaaatt ttagacagca
     1441 tcatttttat tttcaaatta tccgttttac agagtgaggg ttaaatttgc aacacattat
     1501 aaaaacagca ttacaacaaa catttaacta taaaacaaat aaaagtattt acaatatctt
     1561 agttggtaag aaatctcacc aaaccttttt tgacgcttgt agtcaacaac agttgtcatt
     1621 atatcacagt ttaccactat taaaatatcc gtcttttgag ctatttttag aaaaaatcaa
     1681 tgaatttaat gctgaaatgg aaatcatgtt gcatcctaga tatacatttg aaagcatggg
     1741 gcaaacattt caagcaattc aactattagt gcaaaccatg tctaatacca aacaacatgt
     1801 ttttcatttt gtaccaatct ctcaaaataa taagatacaa gaaactgtaa agattgtcta
     1861 taactacatt aaagaaaacc aattacaaat tgattttgaa aatgaactac ataacttgtt
     1921 taaagcaata actttaaaag ggccatgtta cttacattat tatttgcaag gctatgatga
     1981 accaatgtat acgagacagc aagttagttt aatagaaaag ctatctcaac agcaattgtt
     2041 tgaatacgaa atgaataatt tagtgacaat gatgtttgaa ttagaaagtg gagaatatac
     2101 tattttatca aaaataataa tgaaacctac attattaaat caaacttata ttacttatac
     2161 aaaattgctt gaacaattca cgatggaaga tatagcggct caacaacaag ttaaaatcaa
     2221 tactatcgaa gatcatgtac ttgaaatctt aatcaaaggt tacatgtcta attacgatga
     2281 ttatgttgaa ctagaagatc aactccagtt tttgaatttt tatcaacagc atcgtggcga
     2341 acgattaaaa ttttacaaag aacaatttga cacgttatca tattttcaat taaaagtatt
     2401 aatcgttgga tttgaaagag gtgatctgaa tgttgcatga tattttacga aacaaatttg
     2461 gattcgagag ttttaaaccg ggacaacagg aaattataga aagtataatg tctcaacaac
     2521 acactctagg tatacttcca actggaagtg gaaagagttt gtgttatcaa atacctacgt
     2581 atttatcagg taagccgaca ttaattatct caccgttaat atctttaatg gatgaccaag
     2641 ttatgcagtt gaaaataaat ggagaaaaac gtgtaacatg tattcactct ggtatggatg
     2701 aaattgagaa aaagcataat attaaatgtt tacgacatag ccgcttcatc tttctaagtc
     2761 cagaatttct cctgcaaccg tcaaatttta aattaatatc tatgatagac tttggcatga
     2821 ttgttctaga tgaagcacat tgcctatctg aatggggata tgatttcaga ccacattatg
     2881 ctctaatagg aaaagtaaca aagcatttta aagaagcggt tgtcttagca ttgacagcaa
     2941 ctgcaccacc gcatttacaa gatgatttga cggaaatgtt agcgattcaa ttcaatgtta
     3001 ttaaaactac aatgaatcgc ccaaatataa gctttaagca tcttaatttt catgatgatg
     3061 aagataaaat tgaatggttg ctgccgtttc tacaacagtc gggaccaacg attatttatg
     3121 tctcatcgaa aaagatgtgt ctgaatttag cgcaacttat ttatgattca ggttttctta
     3181 caggtattta tcatggtgat atgaattatc aagagcgaca cacagttcaa caacaatttt
     3241 taaataatga tattccgatt atagtcgcaa cgagtgcttt tggtatggga attaataaaa
     3301 aagatattcg cacaatcatt cactttcatc tttcaacaag tccttctaac tacattcaag
     3361 aaattggccg tgcgggtcgc gatggtgaac taagtcaggc aattagttta ttccaaccgg
     3421 acgataaata tattttagaa acgttattat ttgcagatat gataacagaa gaagatgtac
     3481 aaaatttcga aataggagaa tttttagctc ccgataaaca agccgttttg acaacgttgc
     3541 aatcattcta tagtatcggc gccttgaaac agatatttaa gcaatcattt aaacgaaagc
     3601 aattaggatt ctttcgcatg attggctatt gcaaattgga tcaatgtaga cggaagtatt
     3661 tattagaatt tttcggtgaa tatccaccgg cacaagatcg atgttgtgac aatgattcta
     3721 atataactga tatcgcaatt ttaaataaga agaaggtaat tagaagtatt ggatttgatg
     3781 aaaagttgca aaatttattt ctcagatagt attactttac taaaagaaaa ttgacaagct
     3841 ataattagtg tatacacaat tgaaaaatga ttgaaataat tttgaaaaat atacataaac
     3901 atatgtcatg tgggtatatt ttatgtaaaa tcattgtaat agaatagaaa ggaagatggc
     3961 tatgtctaat aattttaaag atgactttga aaaaaatcgt caatcgatag acacaaattc
     4021 acatcaagac catacggaag atgttgaaaa agaccaatca gaattagaac atcaggatac
     4081 aatagagaat acggagcaac agtttccgcc aagaaatgcc caaagaagaa aaagacgccg
     4141 tgatttagca acgaatcata ataaacaagt tcacaatgaa tcacaaacat ctgaagacaa
     4201 tgttcaaaat gaggctggca caatagatga tcgtcaagtc gaatcatcac acagtactga
     4261 aagtcaagaa cctagccatc aagacagtac acctcaacat gaagaggaat attataataa
     4321 gaatgctttt gcaatggata aatcacatcc agaaccaatc gaagacaatg ataaacacga
     4381 tactattaaa aatgcagaaa ataacactga gcattcaaca gtttctgata agagtgaagc
     4441 tgaacaatct cagcaaccta aaccatattt tacaacaggt gctaaccaat cagaaacatc
     4501 aaaaaatgaa catgataatg attctgtaaa acaagatcaa gatgaaccta aagaacatca
     4561 taatggtaaa aaagcagcag ctattggtgc tggaacagca ggtgttgcag gtgcagctgg
     4621 tgcaatggct gcttctaaag ctaagaaaca ttcaaatgac gctcaaaaca aaagtaattc
     4681 tggcaaggcg aataactcga ctgaggataa agcgtctcaa gataagtcta aagatcatca
     4741 taatggcaaa aaaggtgcag cgatcggtgc tggaacagca ggtttggctg gaggcgcagc
     4801 aagtaaaagt gcttctgccg cttcaaaacc acatgcctct aataatgcaa gccaaaacca
     4861 tgatgaacat gacaatcatg acagagataa agaacgtaaa aaaggtggca tggccaaagt
     4921 attgttacca ttaattgcag ctgtactaat tatcggtgca ttagcgatat ttggaggcat
     4981 ggcattaaac aatcataata atggtacaaa agaaaataaa atcgcgaata caaataaaaa
     5041 taatgctgat gaaagtaaag acaaagacac atctaaagac gcttctaaag ataaatcaaa
     5101 atctacagac agtgataaat caaaagagga tcaagacaaa gcgactaaag atgaatctga
     5161 taatgatcaa aacaacgcta atcaagcgaa caatcaagca caaaataatc aaaatcaaca
     5221 acaagctaat caaaatcaac aacagcaaca acaacgtcaa ggtggtggcc aaagacatac
     5281 agtgaatggt caagaaaact tataccgtat cgcaattcaa tactacggtt caggttcacc
     5341 ggaaaatgtt gaaaaaatta gacgtgccaa tggtttaagt ggtaacaata ttagaaacgg
     5401 tcaacaaatc gttattccat aatataacta tataaattgt aactgaactg ctatatacaa
     5461 acgtgcatat tataagtatc tctaaatatt ttggagaact taaattgcgc ttataagtat
     5521 gtagcggttt tttcattttt caaagtttgt tatttaacaa ggtcttttct cgaatattgg
     5581 catatcaatt taacttttta aatagtcatc aaaaagataa aacaccacaa tcaacaaatt
     5641 taacgaggaa gaataaaaaa taaatcaaca tattaaattg tagtgttatt caactccgta
     5701 gctaacaatt ctctatgcac attaaacaaa ttgtcaaaaa tatatcataa atcttcaagc
     5761 acagacttag cgcatcaatc actgaactgt tataatagtt tgggattaaa ggaggccgaa
     5821 acaatgcaaa aagttgaaag tatcataatt ggtggagggc catgcggatt aagtgcggct
     5881 attgaacaaa aaagaaaagg tattgatacc ttaattattg aaaagggtaa tgtcgttgaa
     5941 tcaatctaca attatcctac tcaccaaaca tttttctcat caagtgataa attaagtatt
     6001 ggggacgtac cgtttatcgt tgaagaaagt aaaccaagac gtaatcaagc gctagtttat
     6061 taccgagaag ttgtaaaaca tcatcaatta aaagtaaatg catttgaaga agtattaact
     6121 gttaaaaaaa tgaataataa atttactatt actacgacga aagatgttta tgaatgtcga
     6181 tttttaacaa tcgcgacagg ctattatggt cagcataata cattagaagt tgaaggtgcg
     6241 gatttaccta aagtgttcca ttattttaaa gaggcacatc cgtattttga tcaagatgtt
     6301 gtaattatcg gtggtaagaa ttcggctatc gatgctgctt tggagttgga aaaagctggt
     6361 gctaacgtga cggttctata tcgtggtgga gattattcgc cttcaattaa accgtggata
     6421 cttccaaatt tcacagcatt agtaaatcat gaaaaaattg acatggaatt taatgctaat
     6481 gttacccaaa taactgaaga tactgtgact tatgaagtaa atggtgaaag taaaacgata
     6541 cacaatgatt atgtatttgc gatgattggt tatcatcccg attatgaatt tttaaaatct
     6601 gtaggcattc aaattaatac aaatgaattt ggaacagcgc ctatgtataa taaagaaaca
     6661 tacgaaacaa atatcgaaaa ttgctatatt gcaggtgtaa ttgctgcagg gaacgatgcg
     6721 aataccattt ttattgaaaa tggtaaattc cacgggggca ttattgctca aagcatgcta
     6781 gctaagaaac aaacgccctt agaatcataa aaataaaggt ctatgtacta aataacttag
     6841 ttttacaacg actgacattc atgatatgtc aaatgaggtt gatgactaat gattgtacat
     6901 agaccttttt atgttacgta ttcattataa ttcaaaatat gatttgattt cagctttatc
     6961 taaattgttg cttaacgcga ctaataattt taatcttgct tttggaccat tcaagccgtt
     7021 agaaaaaata aaaccttgtt gtgcgagttg gtaaccacca ccatcgtatg cgtaagttgg
     7081 actcacaata ccattaaagg aacgtgaaac tagcacaata ggtatattta aagatactaa
     7141 ttgttgaatg ccttctaatg cgcttggagg tatgttgcct tgtcctaacg cttcaataac
     7201 cataccatcg ataccttctc gactataaaa actaaaaatg tcacctggca tacccatata
     7261 tgcttttact aatggtacat ttagtttgtc attgacattt tccaatgctt gttggcgata
     7321 tggcatatga tggaattgca cacgatcctt tgtcaataca cctagcggac catgatttgg
     7381 actttgaaat gtgtttgtat tagacgtatg tgttttggta acattacgcg ccgtatgaat
     7441 ttcatcatta aatacaacca tcacgccttt atgacgggcc ttttcatcag aggcaacgcg
     7501 aatagcggaa atataattat ataatccgtc agaaccaatt tcattagacg agcgcattgc
     7561 gccagtaata acaacaggtt gctcgatacc taatattaaa tcaagtaaaa aggcagtttc
     7621 ttctaacgta tcggtaccat gcgtgataac gaaaccatca taatatttat ttgttactgc
     7681 ttctaaaata atatccttta attgtttaac atgttggatt gtcatatgag gtgatggtac
     7741 attaaaagga tttaattcat ctatttgtgc atattgattt atgacatctt gatgcattga
     7801 aatagggttg atatcatttg ttactacttt attagattgg tcttgtgaca tactaatggt
     7861 gccaccagta tgaataacaa gtagatgttt catatatttc ctcctatatt taatttacct
     7921 aattatgata aaatattatt cataaaacga caaggaaggg aaatgacgca tgaaagccat
     7981 taatattgca ttagatggtc cagctgctgc cggaaaaagt acaattgcga aacgtgtagc
     8041 cagcgaacta tcaatgattt atgtcgatac aggagcaatg tatcgtgcat taacatacaa
     8101 atatttaaaa ttaaacaaaa ctgaggactt tgcaaaacta gttgaccaaa caacattaga
     8161 tttaacttat aaagcagata aaggtcaatg tgtcatttta gataacgaag atgtaacaga
     8221 ctttttaaga aataatgatg tgacgcaaca tgtttcatac gttgcatcta aagagccagt
     8281 acgttcattc gccgttaaaa aacaaaaaga gttagctgca gaaaaaggta tcgtaatgga
     8341 tggtcgcgat atcggaactg tagtgctacc agatgcagat ttaaaagtat atatgattgc
     8401 atcagttgaa gagcgagcag aaagaagata taaagataat caattaagag gtatcgaatc
     8461 aaattttgaa gatttaaaac gtgatattga agctcgtgat caatatgaca tgaaccgtga
     8521 aatatcacca ttaagaaaag cagatgatgc agtgacatta gatacgacag gcaagtcgat
     8581 tgaagaagtt actgacgaaa ttttagcgat ggtgagtcaa attaaataat ctaataagtc
     8641 ataactatct atttgtaatg tgcaacgctt aacgcatata caaaatgaat gt
//
LOCUS       DAMQYT010000107         8643 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMQYT010000107 DAMQYT010000000
VERSION     DAMQYT010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8643
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.107"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..614
                     /locus_tag="PGA33_001781"
     CDS             <1..614
                     /locus_tag="PGA33_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDG6473586.1"
                     /translation="FANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNEAQEV
                     TKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHRTDCP
                     NIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLIKVSG
                     RSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            626..1078
                     /locus_tag="PGA33_001782"
     CDS             626..1078
                     /locus_tag="PGA33_001782"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDG6473587.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            1075..1950
                     /locus_tag="PGA33_001783"
     CDS             1075..1950
                     /locus_tag="PGA33_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDG6473588.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            2411..3673
                     /locus_tag="PGA33_001784"
     CDS             2411..3673
                     /locus_tag="PGA33_001784"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDG6473589.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            3689..5455
                     /gene="aspS"
                     /locus_tag="PGA33_001785"
     CDS             3689..5455
                     /gene="aspS"
                     /locus_tag="PGA33_001785"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDG6473590.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            5533..5723
                     /gene="ssrS"
                     /locus_tag="PGA33_001786"
     ncRNA           5533..5723
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PGA33_001786"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5788..5916
                     /locus_tag="PGA33_001787"
     CDS             5788..5916
                     /locus_tag="PGA33_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473591.1"
                     /translation="MGFIAFNKCFLVEFWENNQSDNAKYKILSYYVLMYKSVKEVK"
     gene            5916..6689
                     /locus_tag="PGA33_001788"
     CDS             5916..6689
                     /locus_tag="PGA33_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDG6473592.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(6850..8124)
                     /locus_tag="PGA33_001789"
     CDS             complement(6850..8124)
                     /locus_tag="PGA33_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDG6473593.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDDENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            8209..8631
                     /locus_tag="PGA33_001790"
     CDS             8209..8631
                     /locus_tag="PGA33_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDG6473594.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
BASE COUNT         3024 a         1227 c         1704 g         2688 t
ORIGIN      
        1 actttgcaaa tgaagatgat ttattcgcag ctgtaggatt tggcggcgtg acatccttac
       61 agattgttaa taaattaact gaaagacaac gtattttaga taaacaacgt gctttaaatg
      121 aagcacaaga agttacgaaa tcattgccta ttaaagacaa catcattact gatagtggtg
      181 tctatgtaga aggtttagaa aatgtactta tcaagttgtc aaaatgttgt aatcctatac
      241 caggtgatga tattgtaggt tatatcacca aaggtcacgg tattaaagta catcgcactg
      301 attgcccaaa tattaagaac gaaactgaac gactaattaa tgttgaatgg gtaaaatcaa
      361 aagacgcaac tcaaaaatat caggttgatt tagaggtaac tgcgtatgac cgaaatggct
      421 tgttgaatga agtactacaa gctgttagct cgacagccgg caatttaatt aaagtttcag
      481 gacgttcaga tattgataaa aatgcaataa taaatattag tgtcatggtg aaaaacgtga
      541 atgatgttta tcgtgtggta gaaaagatca aacaacttgg tgatgtttat acagtaacaa
      601 gagtttggaa ctagaggtgc aaaatatgaa agtagttgta caaagagtta aagaagcatc
      661 ggtgacgaat gatacattaa ataatcaaat caaaaaagga tattgtttat tagtcggtat
      721 cggtcagaac tctacagagc aagatgcaga tgtaattgca aagaaaattg ctaatgcaag
      781 attatttgaa gatgacaata ataaattaaa ctttaatatc caacaaatga atggtgaaat
      841 actatcagtt tcacaattta ctctctatgc agatgtaaaa aaaggtaacc gtccaggttt
      901 ctcaaattct aaaaatcctg atcaagcggt aaaaatttat gagtatttta atgatgcgct
      961 acgagcgtat ggtcttactg tgaaaacagg tgaatttgga acacacatga atgttagcat
     1021 aaataatgat ggtccagtca ctattattta tgaaagtcag gacggcaaaa ttcaatgaaa
     1081 aaaatagagg catggttatc taaaaagggt cttaaaaata aacgtactct aatagtagtg
     1141 attgcctttg tcttatttat catcttttta tttttattgc tgaatagcaa tagtgaagat
     1201 agtgggaaca tcacgataac tgaaaatgct gaattacgta caggtccaaa cgctgcgtat
     1261 ccagtcatat ataaagttga aaaaggtgac cattttaaaa agattggtaa agtaggtaaa
     1321 tggattgaag ttgaagatac atccagtaat gaaaaaggtt ggatagctgg atggcacaca
     1381 aatttagata ttgtcgcgga taatacgaag gagaaaaatc ctttgcaagg taaaacaata
     1441 gtgcttgatc ctggtcatgg aggtagtgac cagggtgctt caagcaatac taaatataaa
     1501 agtttagaaa aagattatac gttgaaaaca gcaaaagaat tgcagcgtac tttagaaaaa
     1561 gaaggcgcaa ctgttaagat gacaagaaca gacgatacat atgtttcact agaaaatcgt
     1621 gatatcaaag gcgatgccta tttgagtata cataatgatg cgttagaatc atctaatgca
     1681 aatggaatga cagtttattg gtatcatgat aatcaaagag ctttagcaga tacgttagac
     1741 gctacgattc agaagaaagg tctactttct aatcgcggtt caagacaaga aaattatcaa
     1801 gtgttaagac aaacaaaagt tcctgctgtt ttattagaat taggttatat tagtaaccca
     1861 actgatgaaa cgatgattaa agatcaatta catagacaaa ttttagaaca agcaattgtt
     1921 gatggcctta aaatttattt ttctgcgtag ggcttgcaaa aatatgtgaa agtagttatc
     1981 attgatattg aattttataa ctaaaaccgt tagtattctt gaaatggtaa atgaaatagg
     2041 tagcaatcta actaagattg tgtaggaata taatccatag actgaaagat tatgctgagt
     2101 agtttatata cattgaacac aagaagaggt gctttatgaa aagtaaagcc gttaaacgta
     2161 cgttaaacgt tttgagtggg tttattaaat gcacgcttat aaaaagtaat gatgattaca
     2221 attaggcatg ttttttaaac caatgagggt ggcaacacgg atattcgtcc cttgtgcagt
     2281 ttcaggctgt gcaagggctt tttcttttgc ttttaagatt atgatttatc gtgcaaagtt
     2341 aagtggtcgt atatagtttt agttttaaaa aggtaattaa ataaaatagt ttgccgaggg
     2401 agatgtcaaa atgattaaaa tacctagagg gacgcaggat attttacctg aagattcaaa
     2461 gaaatggcgt tacattgaaa atcaattaga tgaattaatg acattttata attataaaga
     2521 aataagaaca ccaatttttg aaagtacaga tctttttgca agaggtgttg gtgattcaac
     2581 cgatgtcgta caaaaagaaa tgtatacatt taaagataaa ggcgatagaa gtattacatt
     2641 aagacctgag ggaacagctg cagttgtgcg ttcatatatt gaacataaaa tgcaaggtaa
     2701 tccaaaccaa ccaattaaac tttattacaa tggaccgatg tttagatatg aacgtaagca
     2761 aaaaggacgc tatcgtcaat ttaatcaatt tggtgtagaa gctattggtg ctgaaaatcc
     2821 tagcgtagat gcagaagtat tagctatggt tatgcatatt tatcaatcat ttggattaaa
     2881 acatttaaag cttgttatta atagtgtagg ggatatggcg tctcgaaaag aatataacga
     2941 agcgttagtg aaacactttg aaccagtaat tcatgaattt tgttcagatt gtcaatcacg
     3001 tttgcataca aatccgatgc gaattttgga ttgtaaagta gaccgtgata aagaagcgat
     3061 taagactgca cctagaatca ctgatttctt aaatgaggaa tctaaggcat attatgaaca
     3121 agtaaaagct tatttagatg atttaggtat tccatatatt gaagatccta acttagttcg
     3181 tggattggat tattatacac atacagcatt tgaattaatg atggataacc ctaactatga
     3241 tggtgccatt acaacgcttt gtggtggtgg ccgttataat ggtttattag aattgctaga
     3301 tggtccaagt gaaacaggta ttggttttgc gctaagtata gaacgattat tgcttgcact
     3361 tgaagaagaa ggtatcgaat tagatattga agaaaactta gatttattca ttgttacaat
     3421 gggtgatcaa gcagatcgat atgctgtgaa gctattaaat catttgagac ataatggtat
     3481 taaagcagat aaagactatt tacagcgtaa aattaaagga caaatgaaac aagcagaccg
     3541 tttaggtgcc aagtttacaa tcgttattgg tgatcaagaa ttagaaaata ataaaatcga
     3601 tgttaaaaat atgacaactg gtgaatctga aacaattgaa ttagacgcat tagtcgaata
     3661 ttttaagaag tagagagggc gttaaaatat gagtaagaga acaacttatt gtggattagt
     3721 tactgaggca tttttaggac aagaaattac attaaaagga tgggttaaca atcgtcgtga
     3781 ccttggtgga ttgattttcg ttgatttaag agatagagaa ggaattgtac aagtcgtgtt
     3841 taatcctgca ttttcagaag aggcattgaa aattgctgaa acagtacgtt ctgaatatgt
     3901 tgtagaagtt caaggtacag ttacgaagcg tgaccctgaa acagttaatc ctaaaattaa
     3961 aactggccaa gttgaagtac aagttacaaa tattaaagtg attaataaat ctgagacacc
     4021 accattttct ataaatgaag aaaatgttaa cgttgatgaa aatattcgat taaaataccg
     4081 ttatttagat ttacgtcgtc aagagttagc gcaaacattt aaaatgagac atcaaattac
     4141 acgttctatt cgtcaatatt tggatgatga agggttcttt gacatcgaaa caccagtact
     4201 aacgaagtca acacctgagg gtgcacgtga ctatttagta ccatctcgtg ttcatgatgg
     4261 tgaattttat gcattaccac aatcaccaca attatttaag caattattga tgattagtgg
     4321 atttgacaaa tactaccaaa tcgtaaaatg cttccgtgac gaagatttac gtgcagatcg
     4381 tcaacctgaa tttacacaag tcgatattga aatgagtttt gtagaccaag aagatgtgat
     4441 gcaaatgggt gaagaaatgc ttaaaaaagt tgttaaagaa gttaaaggcg ttgaaattaa
     4501 tggcgctttc ccacgcatga catataaaga agcgatgcgt cgctatggtt ctgataaacc
     4561 agatacacgt tttgaaatgg aattaattga cgtttctcaa ttaggacgtg atatggactt
     4621 taaagtattt aaagatactg ttgaaaatga tggtgaaatt aaagcaattg tcgctaaagg
     4681 tgcagctgaa caatatactc gtaaagatat ggatgcttta acagaatttg taaacatcta
     4741 tggtgctaaa ggattagcgt gggttaaagt tgtggaagat ggtttgacag gtccaattgg
     4801 acgtttcttt gaaacagaaa atgttgaaac attacttaca ttaactggtg ctgaagctgg
     4861 tgacttagta atgtttgttg cagacaaacc aaatgtcgtt gcacaaagtt taggtgcatt
     4921 acgtgtcaaa ttagctaaag aattaggttt aatcgatgaa acaaaattaa acttcttatg
     4981 ggtgacagat tggccattat tagaatatga tgaagatgcg aaacgttacg ttgcagcaca
     5041 tcatccattt acatctccaa aagaagctga tattgctaag cttggcactg cgccagaaga
     5101 agctgaggca aatgcttatg acatagtatt aaatggttat gaattaggtg gcggttcaat
     5161 cagaattcat gatggtgagt tacaagaaaa aatgttcgaa gttcttggat ttactaaaga
     5221 acaagcacaa gaacagttcg gcttcttact agatgcattt aaatatggtg caccaccaca
     5281 tggcggtatt gcattaggat tagaccgatt agtcatgtta ttgactaata gaacaaactt
     5341 gagagataca atcgcattcc ctaaaacagc atctgctaca tgtttattaa cgaatgcgcc
     5401 tggtgaagtt tctgataaac aattagaaga actttctttg cgaattcgtc actaaatttt
     5461 aaaaaatcaa taaactttgg tttgttataa tttcattaat tttaattatg tggtaatata
     5521 acatcataag atagtcatct gagttgttcg taagtttgct ttttaatttg ggcctaacac
     5581 tctttgatca agggagccca atgggttttc ttgcagcgta cacgccttat atgaaggacg
     5641 tacaaaacaa gaaacagggc acccacctgt atataacagg ccgaatgatc aagctattat
     5701 aactacggca atacggactc tatcgatacg caagactttg tcttgcgtat ttttatgcgt
     5761 ttctttaagt ttttaataaa gaattagttg ggttttatag cgtttaataa atgtttcctc
     5821 gttgaatttt gggaaaataa tcaaagtgat aatgctaaat ataaaatttt aagttactat
     5881 gtacttatgt ataaatcagt gaaagaggta aaataatgaa acatcaattt tcaagaaatg
     5941 aactagcgat tggacaacaa ggtttagatt tattgaagca acaaactgtt gtcatacttg
     6001 gcgttggagg tgttggttca tttgctgctg aagcattagc aagaacgaat attggtcata
     6061 ttatattaat agataaagat gatgttgata ttacaaatgt aaacagacaa ttacacgctt
     6121 taactacaac ggtaggacaa agtaaagttt ctttgatgga agaacgtatt aaattaatta
     6181 atccagattg taaagtcacg cccttacata tgttttatac agaagagaca tatgaagaaa
     6241 ttttcgataa ctacgatata gattattttg tagatgcaag tgatacaata atgtacaaag
     6301 tacacttaat gaaagagtgt cttgaccgag ggattaaagt gatttccagt atgggtgctg
     6361 caaataaaac agatccaaca cgttttacaa tttcagatat ctctaaaaca cataccgatc
     6421 caatggccaa aattattcgt caaaaattga aaaaactagg aattcgtaaa gggattccag
     6481 ttgtattttc agatgaaagt ccaattgtca taagagaaga tgtaaaagat atagttggag
     6541 ataaaaatgc tatcaatcga aaaggacaaa tgccaccttc ttcaaatgcc tttgtgccaa
     6601 gtgttgttgg attaatttgt gcaagttatg tggtgaatga cgtattaaaa gatattccag
     6661 ttcgtcgcat taaagacaaa gggcaataat tcattttgaa agggataatt ttcaacgtaa
     6721 ggcaagtgta accacaccat aaaactaaaa ctgactagtt cgcaagcata gtagaatatg
     6781 cctcgtgtac tagtcagttt tgatttgatt atagctaaaa tatcaaaggt aatcgattta
     6841 atcttatttt tacggccttt gtttaagtaa gttgttatat atttctttga gttgttgttc
     6901 acttttagat gtcgtttttg gttcgtaata aattttgttt tttagtttat caggtaaata
     6961 ttgctgtgaa acatagccat taacatattg gtgtggatat ttgtaaccaa tagatcggcc
     7021 tagatcttta gcaccttgat aatgtccatc ttttaaatgg tttggaattt ggcccacatg
     7081 accgtttcta atatcggaca atgcactatc aatggcactc attgctgaat ttgacttagg
     7141 tgataagcat agttcgatta ctgcttggct tagtggaatt ctagcttctg gtaaacctag
     7201 acgttctgct gattcaatag cagcaagtgt tctctgacca gcattaggtg aggctaagcc
     7261 tatatcctca tagctaatta caagtaatcg tcgaactatt gtaggtaaat ctccagcttc
     7321 aattaatcgt gctaaataat gtaaagcggc attgacgtcg ctaccacgga tagatttttg
     7381 gaaagcgctc ataacatcgt aatgcatgtc accatccttg tcacttacaa atgcaccttt
     7441 ttgtaaacag tctttagcat cttgcaatgt aacatgtcga taaccgtctt tgtcattatc
     7501 tgcgcttaat acagctaatt ccaatgcatt taacgcacta cgaacatcac cttggctttg
     7561 tgtagaaaag taggtcatgg catcttcatc aattttgggt tgatatgttt tcaaaccatt
     7621 ctcatcatct tctattgcac gagttaacgc ttggcgcaca tcttcgtcat ttaaaggata
     7681 taactcgaaa atttgcgctc ttgaacgaat cgctggattg atagcatgat aaggatttga
     7741 agttgtagca ccgatcaaga cgattttgcc attttctaaa tgaggtaata aaaagtcttg
     7801 tttagcttta tctagtcgat gtatttcatc taataacaag ataacttgac cagacatttt
     7861 agcttcttca acaacaagtt gcatatcttt tttagtgtta gttacagcat tcaattgtct
     7921 gaatttatat tgcgtactgc ccgaaattgc tttggcaata cttgttttgc ctataccagg
     7981 tggaccataa aaaatcattg aagttaattt ttttgtatca accattcttc tgataatgcc
     8041 tcttggtcca actaaatgtt gttgggaaat gatttcatct atattttttg ggcgcattct
     8101 cgatgctaat ggttctgtac tcacatttgt cacacctttc ttatactatc ttaactcaaa
     8161 aaatgctaaa atgggaatat caatattcga attaagtgag gttatacaat gaaaatttct
     8221 actaaaggga gatatggact tacattgatg atttctcttg ctaaaaaaga ggggcaagga
     8281 tgtatatcat taaagtcaat tgctgaagaa aataatttga gtgatttata tttagaacag
     8341 cttgtaggtc ctttaagaaa tgcggggtta attcgaagtg tacgcggtgc taaaggtgga
     8401 taccaattaa gagtgccagc ggaagaaatc tcagcagggg atattataag actgttagaa
     8461 ggtccaatta catttgttga aagtattgaa tcagaaccac ctgcgcaaaa acaactatgg
     8521 attcgcatga gagatgcagt gagagatgtt ttagataata caacattgaa atatttagcg
     8581 gaatatgtag atacaagtga agatttagac ggatacatgt tttatattta atcgtataaa
     8641 tac
//
LOCUS       DAMQYT010000108         8495 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMQYT010000108 DAMQYT010000000
VERSION     DAMQYT010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.108"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..365
                     /gene="modA"
                     /locus_tag="PGA33_001791"
     CDS             <1..365
                     /gene="modA"
                     /locus_tag="PGA33_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDG6473595.1"
                     /translation="DKLALGEVKTVPAGKYAKQYLDNNNLFKEVESKIVYAKDVKQVL
                     NYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEVELKKPITYEAGATSDSKLAKEWME
                     FLKSDKAKEILKEYHFAA"
     gene            379..1050
                     /gene="modB"
                     /locus_tag="PGA33_001792"
     CDS             379..1050
                     /gene="modB"
                     /locus_tag="PGA33_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDG6473596.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKLLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            1051..1656
                     /locus_tag="PGA33_001793"
     CDS             1051..1656
                     /locus_tag="PGA33_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDG6473597.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            1826..2830
                     /locus_tag="PGA33_001794"
     CDS             1826..2830
                     /locus_tag="PGA33_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HDG6473598.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKYRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            2861..3367
                     /locus_tag="PGA33_001795"
     CDS             2861..3367
                     /locus_tag="PGA33_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HDG6473599.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     DGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(3369..3863)
                     /gene="moaC"
                     /locus_tag="PGA33_001796"
     CDS             complement(3369..3863)
                     /gene="moaC"
                     /locus_tag="PGA33_001796"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDG6473600.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTLIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSNQ"
     gene            3926..5185
                     /locus_tag="PGA33_001797"
     CDS             3926..5185
                     /locus_tag="PGA33_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDG6473601.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETKTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFVDGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            5182..5667
                     /gene="mobB"
                     /locus_tag="PGA33_001798"
     CDS             5182..5667
                     /gene="mobB"
                     /locus_tag="PGA33_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDG6473602.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            5681..6127
                     /locus_tag="PGA33_001799"
     CDS             5681..6127
                     /locus_tag="PGA33_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HDG6473603.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            6133..6366
                     /gene="moaD"
                     /locus_tag="PGA33_001800"
     CDS             6133..6366
                     /gene="moaD"
                     /locus_tag="PGA33_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HDG6473604.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            6373..6972
                     /gene="mobA"
                     /locus_tag="PGA33_001801"
     CDS             6373..6972
                     /gene="mobA"
                     /locus_tag="PGA33_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDG6473605.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            6992..8014
                     /gene="moaA"
                     /locus_tag="PGA33_001802"
     CDS             6992..8014
                     /gene="moaA"
                     /locus_tag="PGA33_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDG6473606.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(8295..8465)
                     /locus_tag="PGA33_001803"
     CDS             complement(8295..8465)
                     /locus_tag="PGA33_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473607.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNGMECDINVKEPLKIYYV
                     VILKSGIEMIFS"
BASE COUNT         2963 a         1203 c         1713 g         2616 t
ORIGIN      
        1 atgataaatt agcattaggt gaagtgaaaa ctgtaccagc aggaaaatat gcgaaacagt
       61 atttagataa caataactta tttaaagaag tcgaaagtaa aatcgtttat gctaaagatg
      121 taaaacaagt attaaattat gttgaaaagg gtaatgcgaa acaaggtttt gtgtataaaa
      181 ctgacttata taaacaaaat aaaaaaattg atactgtaaa agtaattaaa gaagtagaac
      241 ttaagaagcc aatcacatac gaagctggtg ctacatcaga tagtaaatta gcaaaagagt
      301 ggatggaatt cttaaaatca gataaagcta aagaaatact aaaagaatac cactttgcag
      361 cataaggagt tgtaatccat gcctgactta acaccttttt ggatatcaat acgagttgct
      421 gtaatcagta cgattattgt aacggtttta ggtattttta tatctaaatg gttgtatcgt
      481 cgtaagggtt cgtgggttaa attattggaa agtttattga tattacctat tgttttgccg
      541 ccaacggtat taggttttat tctattaatc atcttctcgc caagaggacc aatcggtcaa
      601 ttctttgcga atgtactaca tttacctgta gtgttcactt tgacaggtgc tgtgatagca
      661 tctgtcattg ttagttttcc actaatgtat caacatactg tgcaaggctt cagaggtata
      721 gacacgaaaa tgattaatac agctagaacg atgggagcaa gtgaaacgaa aattttcctc
      781 aaattaattt taccattagc taaacgctct attttagcag gtataatgat gagttttgct
      841 cgtgcattag gtgagtttgg tgctacatta atggttgcag gatatattcc aaataaaacg
      901 aatacactac ctttagaaat atacttctta gtggaacaag gtagagaaaa tgaagcgtgg
      961 ttatgggtat tagtgctagt cgcattctct attgtggtta tatctacaat taatttattg
     1021 aataaagata aatataagga ggtcgactag atgcttaaaa tcaatgtgaa atatcaatta
     1081 aagaacactt taattcgcat caatatagat gatactgaac caaaaattta tgcagttcgt
     1141 ggtccatctg gcattggtaa aactactgtt ttaaatatga ttgccggatt acgtaaagca
     1201 gatgaagcta ttatcgaagt gaatgggcaa ttacttactg atacggcaaa aaacgtgaat
     1261 gttaaaattc aacaacgacg tattggatat ctgtttcaag actaccaatt gtttcctaat
     1321 atgacggtct ataaaaatat tacttttatg gctgaaccat ctgaacacat cgatcaatta
     1381 attcaaactt taaacattga tcatttgatg aaacaatatc ctatgacatt gtcaggtgga
     1441 gaggcacaac gtgtagcact tgcacgtgca cttagcacga aaccagattt aattttatta
     1501 gatgaacctt tttctagttt ggatgatact acaaaagatg agagtattac attagttaaa
     1561 cgtattttca acgaatggca aataccaatc atatttgtga cacattcaaa ctatgaagca
     1621 gaacaaatgg ctcatgaaat tattacaatt gggtaatcat ttatttgcca ttaaagagtt
     1681 tagaacgtat ttaaaattgt agaagtgaat gcttctatca gcattttaat gatgttttaa
     1741 actctttttt aggggcagtt tttttgagag acattgacgc gcgtcatata atgaaagtaa
     1801 tgataaaaag aaaggataac ttaatgtgag tcaagaacgt tattcaaggc aaattttatt
     1861 taaacaaata ggtgaaatag gtcaaagcaa aataaatcaa aaatgtgcgt tgattattgg
     1921 tatgggcgca ttaggtacac atgtggccga aggacttgtt agagcaggca ttgccaaact
     1981 aatcattgtt gatagagatt atattgaatt tagtaattta caaagacaaa cattgtttac
     2041 tgaagaagat gctttgaaaa tgatgcctaa ggtggttgca gctaaaaagc atttgctagc
     2101 gttacgtagt gatgttgata ttgatgatta tattgcccat gtggattatt attttttgga
     2161 aacacatgga caggacgttg acgttattat tgatgcaacc gataactttg aaacacgaca
     2221 actgattaat gattttgcat ataaatatcg tataccttgg atttatggtg gtgttgtaca
     2281 gagtacatat acagaagctg catttatacc tggtaaaaca ccttgcttta actgtttggt
     2341 accacaattg ccagcattaa atttaacatg tgatacagta ggggtcattc aacctgccgt
     2401 gacgatggca acaagtttac aattaagaga tgcgatgaaa gtattaacgg aacaaccaat
     2461 tgacacaaaa ataacttatg gcgatatttg ggaaggtagt cattattcat ttggtttcag
     2521 taaaatgcaa cgttcagact gtacaacttg tggagatgta ccaagttatc cgtatttaaa
     2581 caagaatgaa caacgttatg caacattgtg tggtagagac actgtacagt atgaaaatgc
     2641 atcaattaca cacgacattc ttgttcaatt tttaaaacaa catcagttaa attatcgcag
     2701 taattcgtat atggttatgt ttgaatttaa aggacaccgc attgttgctt ttaaaggtgg
     2761 aaggttttta atacatggca tgacacgcac atcagatgcc acacatctaa tgaatttatt
     2821 gtttggataa aaaaagataa gacaaaagga gtgtaatatt atgggcgaac atcaaaacgt
     2881 taaattgaat cgtacagtta aagcagccgt actaacggta tcagatacta gagactttga
     2941 tacagataaa ggtggtcaat gcgtgcgcca actattacaa gcagatgacg ttgaagtgag
     3001 tgacgcacat tatacaattg tgaaagatga aaaagtagcc atcacgacgc aggtgaagaa
     3061 gtggttagaa gaagatattg atgtcatcat tacgactggt ggaacaggta ttgcacaacg
     3121 tgatgtgacg attgaagcag taaaaccact tttaactaaa gagatagatg gctttgggga
     3181 attgtttaga tatttgagtt atgttgaaga tgttggcacg cgtgcattat tgtctcgtgc
     3241 tgtagcaggt acagttaata ataaattgat attttcgatt ccaggatcaa caggcgcagt
     3301 taaattagca ttagaaaagc tcattaaacc agaattaaat catctgattc atgagcttac
     3361 aaaataattt attgatttga ttggcgttga aaatctccag atttaccgcc agacttgctt
     3421 tcaaggtagg tttcgccaat aatcatacct ttatcaactg ctttcgtcat gtcgtaaatg
     3481 gttaaagccg ttgctgatgc agcggttaaa gcttccattt caacaccggt tttgccagtt
     3541 gtagagacag ttgtttgaat gtttaaagta taaaggggtg catttgtttc atcccagctg
     3601 aagtgaacat ctatgccagt caatggtaat ggatggcaca tcggaataag tgttgatgta
     3661 tttttggcag ccataatacc agcgatttga gcagtgttca atacattgcc tttggtattg
     3721 gtgttattag aaatctgctt aaaaatagtt tcatttacag taatgcttga atgagcgaca
     3781 gcagttcttt ttgtaatttg tttgtctgat acatcgacca ttttggcgtg gccttgttga
     3841 ttaatatgag taaactcagt cattttaccc ctcctagtgc atctagtata tcatgaaaaa
     3901 ataaaagttt tggagatgat ttttaatggt agtagaaaaa agaaacccaa tcccagttaa
     3961 agaagcaatt caacgtatcg ttaatcagca gagttcaatg ccggcaatta cggtagcact
     4021 tgaaaaaagt ctaaatcata tcttagcaga agatattgta gctacttatg atataccaag
     4081 gtttgataaa tcaccttatg atggttttgc aattcgcagt gttgattcac aaggggcaag
     4141 tggtcagaat cgcattgagt ttaaagtgat tgatcatatt ggtgcaggtt cagtttctga
     4201 taaattagtt ggggatcacg aagcggtgcg tattatgact ggagcacaaa tacctaatgg
     4261 cgcagatgct gttgttatgt ttgaacaaac gattgaacta gaagatacat ttacaattcg
     4321 taaaccattt tcaaaaaatg aaaatatatc tttaaaaggt gaagaaacaa agacaggcga
     4381 tgttgttcta aaaaaaggac aagtaattaa tccaggggct atcgcggtcc ttgcaacata
     4441 tggctatgca gaggttaaag ttattaagca accgagtgtc gctgttattg caacaggaag
     4501 cgaattatta gatgttaatg atgtattaga agatggaaaa attcgtaact ctaatggccc
     4561 aatgattcgt gccttagcag aaaaattagg tcttgaagtt ggtatttaca aaacacaaaa
     4621 agatgattta gatagtggca tccaagtcgt taaagaagct atggaaaaac atgatatcgt
     4681 tattacaacg ggcggagttt ctgttggaga ttttgactat ttacctgaga tttataaggc
     4741 tgtaaaggcg gaagtgttat ttaataaagt agcaatgcgt cctggtagcg taacaacggt
     4801 tgcatttgta gatggaaagt atttgtttgg attatctgga aatccatcag cttgttttac
     4861 aggatttgaa ctatttgtga aaccagctgt taaacatatg tgtggcgcac tagaagtctt
     4921 cccgcaaata attaaagcaa cattaatgga agattttacc aaggcaaacc cattcacacg
     4981 atttatacgt gctaaagcaa cgttaacaag tgctggagct actgtagtac cttcaggatt
     5041 caataaatca ggtgcggttg tagcgattgc acatgctaac tgtatggtca tgttaccagg
     5101 agggtcacgt ggttttaaag cggggcatac agtagatatt atattgactg aatctgacgc
     5161 tgctgaagag gaacttcttt tatgatttta caaattgtag gttacaaaaa gtctggtaag
     5221 acaacattga tgaggcatat tgtctctttc ttaaagtcac atggttatac agttgctact
     5281 attaaacatc atgggcatgg taaggaagat attcaattac aggattcaga cgtcgatcac
     5341 atgaagcatt ttgaagcggg ggcagatcaa agtattgtac aaggttttca atatcagcaa
     5401 actgtaacac gtgtagataa tcaaaatctt actcaaatta ttgaaaaatc tgttacaatt
     5461 gacaccaata tcgtattagt tgaaggcttt aaaaatgctg attttgaaaa agtcgtagtc
     5521 tatcgaaatg aagaagagtt gcaagtatta caacaattgt cgaatgtttg ttatagcatt
     5581 aatgtaaggg agcatgaaga ttttacagca tttgagcaat ggttattaaa taaaattaaa
     5641 aatgattgtg atacacaatt aacatagagg attgaaatga atgaaacaat ttgaaatcgt
     5701 gacagaaccg atacaaacag aacaatatcg tgaattcact ataaatgaat atcaaggtgc
     5761 agtagttgtt tttaccggtc atgttcgcga atggactaaa ggcgtcaaaa cggaatattt
     5821 agaatatgaa gcgtatattc caatggctga aaagaaattg gcacaaattg gagatgaaat
     5881 aaatgaaaaa tggcctggaa cgataacgag tattgttcat agaatagggc cattacaaat
     5941 ttcagatatc gctgtattaa ttgcggtttc ttcaccgcat cgtaaagatg cctatcgagc
     6001 aaatgaatat gcaattgagc gtataaaaga aattgttccg atttggaaaa aagaaatttg
     6061 ggaagatggt tcaaaatggc aagggcatca aaaagggaat tatgaagaag cgaagaggga
     6121 ggaataagag agatgaaggt actttacttc gcagaaatta aagatatatt acaaaaagca
     6181 caggaagata ttgtgcttga acaagcattg actgtacaac aatttgaaga tttattgttt
     6241 gaacgttatc cgcaaatcaa taataaaaag tttcaagttg ctgtaaatga ggaatttgta
     6301 caaaaatcgg atttcattca acctaatgat actgttgcat taattccacc ggttagtgga
     6361 ggttaaggga gcatgaaagc aataattctt gcaggtggtc attcagtgcg atttggtaag
     6421 cccaaagctt ttgcggaagt gaacggtgag accttttata gtagagtaat taagacatta
     6481 gaatcaacaa atatgttcaa tgaaattatt attagtacaa atgcgcaatt ggcaacgcaa
     6541 tttaaatatc caaatgttgt tatagatgat gagaatcata atgataaagg tccattagca
     6601 ggaatttata caatcatgaa gcaacatcct gaagaagaat tgttttttgt cgtttctgtt
     6661 gatacaccaa tgattactgg taaagctgta agcacgttgt atcagttttt agtttctcat
     6721 cttattgaaa atcatttaga tgtcgcagct tttaaagaag atggacgttt tattccaaca
     6781 attgcatttt atagtccgaa tgcattaggc gctataacta aagcactaca ttctgataat
     6841 tacagtttta aaaatgtata tcatgaatta tcaacggatt atttggatgt aagggatgta
     6901 gatgcgccct catattggta caaaaatata aattatcagc atgatttgga cgctttaatt
     6961 caaaaattgt aagctgttag gaggtccaca aatggtagaa caaataaaag ataaactagg
     7021 acgtcccatc cgtgacttac ggttatctgt gacagatcgg tgtaacttta ggtgtgatta
     7081 ttgcatgcct aaagaggtat ttggagatga tttcgtattt ttacctaaaa atgaactttt
     7141 aacgtttgat gaaatggcta gaatcgctaa ggtatatgca gaattaggtg taaaaaaaat
     7201 acgcattaca ggtggagaac cattgatgcg acgggattta gatgtactta tagctaaatt
     7261 aaatcaaatc gatggtattg aagatattgg tttgactaca aatggtttgt tattaaaaaa
     7321 gcatggacaa aagttatatg atgctgggct gcgcagaatt aatgtcagtt tggatgctat
     7381 tgatgatacg ctatttcaat caatcaataa tcgtaatatt aaagcgacta cgattttaga
     7441 acaaattgat tacgcgacgt ctattggttt gaatgtaaaa gtaaatgttg ttatacaaaa
     7501 aggtattaac gatgatcaaa tcataccaat gcttgaatat tttaaagata aacatataga
     7561 gattcgattt atagaattta tggatgttgg taatgataat ggatgggatt tcagtaaagt
     7621 tgtaactaaa gatgaaatgc ttacaatgat agagcagcac tttgaaatcg atcctgtaga
     7681 accaaaatat tttggggaag tagcaaaata ttatcgccat aaggataatg gtgttcaatt
     7741 tggtttgatt acaagtgttt cacaatcatt ttgttctaca tgtacacgcg caaggctgtc
     7801 atcagatggg aagttttacg gatgtttatt tgcaactgtc gatggattta acgttaaagc
     7861 gtttattcgt tctggcgtga ccgacgaaga attaaaagaa caatttaaag ctttatggca
     7921 aataagagat gatcgatatt cagatgagag aactgctcaa acagttgcca atcgtcaacg
     7981 taaaaagata aacatgaatt atattggtgg ttaatgtgta gggaccacta catattaaat
     8041 cattagagat gttttaatat ttctgtctta ctccctaaaa tacaatatta tttattaaag
     8101 taaaaacggt catatctatg ccagatttaa tagaaatgat cgtttttaaa gtttttacaa
     8161 gttggcgggg ccccaacaca gaagctgaca gaaagtcagc ttacaataat gtgcaagttg
     8221 gcggggcccc aacatagaga atttcaaaaa gaaattctac agacaatgca agttggggat
     8281 caacgaaata aattttatga gaatatcatt tctatcccac tcttaagaat cactacataa
     8341 taaatcttta gtggttcttt aacattgatg tcacactcca tgccattgag ttgtaatata
     8401 tcttttttag gtataaatgt tgtcgaataa acaacaagtt gtccaaaaga tataaatcta
     8461 aacaagatat agccagcaat ttaatatttg taata
//
LOCUS       DAMQYT010000109         8491 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMQYT010000109 DAMQYT010000000
VERSION     DAMQYT010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.109"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            48..1340
                     /gene="obgE"
                     /locus_tag="PGA33_001804"
     CDS             48..1340
                     /gene="obgE"
                     /locus_tag="PGA33_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDG6473608.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            1353..1808
                     /locus_tag="PGA33_001805"
     CDS             1353..1808
                     /locus_tag="PGA33_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDG6473609.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            1822..2424
                     /gene="ruvA"
                     /locus_tag="PGA33_001806"
     CDS             1822..2424
                     /gene="ruvA"
                     /locus_tag="PGA33_001806"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDG6473610.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            2456..3460
                     /gene="ruvB"
                     /locus_tag="PGA33_001807"
     CDS             2456..3460
                     /gene="ruvB"
                     /locus_tag="PGA33_001807"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDG6473611.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERE
                     "
     gene            3462..4487
                     /gene="queA"
                     /locus_tag="PGA33_001808"
     CDS             3462..4487
                     /gene="queA"
                     /locus_tag="PGA33_001808"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDG6473612.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSSRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            4510..5649
                     /gene="tgt"
                     /locus_tag="PGA33_001809"
     CDS             4510..5649
                     /gene="tgt"
                     /locus_tag="PGA33_001809"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDG6473613.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            5668..5928
                     /gene="yajC"
                     /locus_tag="PGA33_001810"
     CDS             5668..5928
                     /gene="yajC"
                     /locus_tag="PGA33_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDG6473614.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            6203..8482
                     /gene="secDF"
                     /locus_tag="PGA33_001811"
     CDS             6203..8482
                     /gene="secDF"
                     /locus_tag="PGA33_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HDG6473615.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
BASE COUNT         2987 a         1175 c         1694 g         2635 t
ORIGIN      
        1 tataattatt gggaaaatga aaattatatg taaaagaggt gagatatatg tttgtcgatc
       61 aagtcaaaat atctcttaaa gccggtgatg gtggtaatgg tattaccgca tacagaagag
      121 aaaaatatgt accatttggt ggaccagctg gcggtgacgg tggtaaaggt gcttcagtcg
      181 tatttgaagt ggatgaaggt ttaagaacgt tattagattt tagatatcaa cgtcatttta
      241 aagcaagcaa aggtgaaaat ggccaaagta gtaatatgca tggtaaaaat gcggaagatt
      301 tagtattaaa agttccacct ggtacaatta ttaaaaatgt tgaaacagac gaagtgttag
      361 cagatcttgt tgaagatggt caaagagctg tagtagcgaa gggcggtcga ggtggccgag
      421 gtaattcacg ttttgcaaca cctagaaacc ctgcacctga cttcagtgaa aaaggtgaac
      481 caggtgagga attagatgta tctttagaat tgaaattatt agctgatgta ggattagtag
      541 gtttccctag tgtgggtaaa tcgactttat tatctatcgt ttcaaaagct aagcctaaaa
      601 ttggggcata tcattttaca acgattaaac caaatctagg tgttgtttca acgcctgatc
      661 aacgtagttt tgttatggca gatttaccag gtttaattga aggtgcatct gatggcgttg
      721 gattaggaca tcaattttta agacatgtag agagaacaaa agttattgtt cacatgattg
      781 atatgagcgg ttctgaaggt agagaaccta ttgaagatta taaagtcatt aatcaagaat
      841 tagctgcgta cgagcaacgt ttagaagata gacctcaaat cgtagtagct aacaagatgg
      901 atttacctga atcacaagat aatttaaact tgtttaaaga agaaattggc gaagatgtgc
      961 cagttattcc agtttcaaca ataacgcgtg ataatattga tcaattatta tatgcaatag
     1021 cagataaatt agaagaatat aaagatgttg acttcacagt tgaagaagag gagtcagttg
     1081 gcattaaccg agtattatat aaacatacac cgtcacaaga taaatttaca atttcaagag
     1141 atgatgatgg tgcttatgtg gtaagtggta atgctattga aagaatgttt aaaatgactg
     1201 actttaacag tgatccagca gtacgtcgat ttgctcgtca aatgcgttcg atgggtattg
     1261 atgatgcgct tagagaacgt ggttgtaaaa atggtgatat cgttagaatt cttggcggag
     1321 aatttgaatt cgttgaatag gagcgaaaca tgatggacaa taaagattat aaaaagtttt
     1381 atttaattag agaagatgtc ttgcctgaat ccgtggttaa aacattgaag attaaagatg
     1441 ccttaaaaag tgatccgaca ttgtccattt atgatgccgt taaacagttt gatctatcta
     1501 gaagtgcttt ttataaatat agagaaacga tatttccagt agacgataaa atgcttgacc
     1561 atcgagaatt tacattaatt ttatatgtaa ctgatattgt tggtatgttg gcacgtgtac
     1621 tagatgttat atcaaagtta gaactatctg tattaacgat tcatcaaagt attccaatgg
     1681 aagaaaaagc aacaataaca ttatcactga atgctaaatc taaagaaact tcagtagaag
     1741 atgttattgg cgctttgaga aatttagatt atgtatcaaa agtagaatta attagtatga
     1801 gtatgtaagg atgtgcctat aatgtacgcg tatgtcaaag gtaagttaac acatttatat
     1861 cctacacacg tagttgttga aactgctggt gttggttatg aaattcaaac accaaattct
     1921 tatcgttttc aaaagcatct agatcatgaa gttttaattc atacatcttt aattgttcgt
     1981 gaagatgcac aattattgta tggatttagt agtgaagaag agaaagatat gttcttgagt
     2041 ttaattaaag ttactggtat tggtccgaaa tcagctttag ctattttagc gacaagtacg
     2101 cctaatgaag taaaacgtgc cattgaaaat gaaaatgata cgtatttaac taaattccca
     2161 ggaattggta agaaaacggc aagacagatt gtcttagatt taaaaggtaa agtgaaaatt
     2221 actgaagaag atagcgattc attattacaa gtagacgcta cttcgacggt gcaagatcaa
     2281 ttcgtgcaag aagcaatgtt agcgttagaa gcattaggtt attctaaacg agagcttgca
     2341 aaagttgaga aaacgttaaa taaaaataaa tatgactcag ttgatgaagc tgttaaggca
     2401 ggtcttcaat tagttgtatc ttaattttaa atagattaat aggggaagtg ttgtcatgaa
     2461 tgagcgtatg gttgatcaat caatgcatag tgaagaaact gatttcgaat tgtcgcttag
     2521 acctacgaga ttacgacaat atattggtca aaattcaata aaaagtaatt tagaagtatt
     2581 tattaaagcg gctaaacttc gtcatgaacc attagatcat gtattgcttt ttggcccccc
     2641 tggattaggt aagacaacat tatctaatat cattgccaat gaaatggaag ttaatatacg
     2701 tacagtatca gggccttcat tagaaagacc tggtgatttg gctgcaattt tatcaggact
     2761 tcaacctgga gatgttttgt ttattgatga aatacacaga ctgagtagtg ttgttgaaga
     2821 agtgttatac cctgcaatgg aagatttctt tttagatatt atcattggta aaggcgatga
     2881 ggctagaagt atccgtatcg acttacctcc attcactttg gtaggtgcaa caacgcgagc
     2941 tggcagctta acaggtccac taagggatcg atttggtgtg cacttaagat tagaatatta
     3001 taacgaatca gatttaaaag aaatcattat tagaacagct gaggttttag gcacaggtat
     3061 tgatgaagaa agtgccattg aacttgctaa acgttctaga gggactccaa gagtagcaaa
     3121 tcgactattg aagcgggtaa gagacttcca gcaagtgaat gaagatgaac aaatatacat
     3181 tgaaacaacg aagcacgcat taggtttact tcaagttgat caacacggac tagattacat
     3241 tgatcataaa atgatgaact gtattattaa gcagtataat ggcggacctg ttggtttaga
     3301 tacgattgcc gtaacaattg gtgaagaacg tattacaatt gaggacgttt atgagccatt
     3361 tcttattcag aaaggctttt tagaacgtac gccacgtggc agaaaagcaa caccattagc
     3421 ttatgaacat tttgcaaagt cgaatgagga gagagaataa cgtgaatatt gaagaatttg
     3481 actatgactt accagaatca ttaattgctc aaacgccttt aaaagatcgt gatcatagtc
     3541 gtttattagt catggataga gaaactggtg aaatgaaaca tttacatttc aaagatatca
     3601 ttgagtattt tagacctggt gatacattag tgcttaacga tacgcgagta atgccagcta
     3661 gactttttgg tttaaaagaa gaaactggtg caaaagttga aatgttaatg ttaactcaaa
     3721 ttgaaggtaa tgattgggaa gtcttactga aaccagctaa gcgtattaaa gttggtaata
     3781 aattgaattt tggtaatggc aaaattatag ctgaatgcat aaaagaaatg gatcaaggtg
     3841 gacgcatcat gcgtttacat tatgaaggta ttttacaaga aagattagat gaattagggg
     3901 aaatgccact gccaccatac atcaaagaac gtttagatga tccagatcgt tatcaaacag
     3961 tttacgctaa agaaagtggt tcagcggcag caccaacagc aggattacat tttactgatg
     4021 agttattaat tgaaattaaa aataaaggtg ttaatatcgc atttgttaca ttacatgttg
     4081 ggttaggtac gtttagaccg gtgagcgtag acgatgtgaa tgaccacgaa atgcatagtg
     4141 aatattatca aatgacacaa gaaacagctg atttattaaa tgatactaag tccaaaggac
     4201 atcgcattat atcagttggt acaacttcaa cacgtacact tgaaacaatt cgacgcgatc
     4261 atgataaatt tgttgaaacg agtggctgga ctaatatatt tatttatcca ggatttgatt
     4321 ttaaagcaat tgatggccag attactaatt ttcatttacc aaaatcaaca ttagttatgc
     4381 tagtatcagc gtttagtagt cgtgaaaatg ttctgaatgc ttataaaacg gcagtaaatt
     4441 tagaatatag attctttagt tttggcgatg caatgttaat tatataaaaa gaatgtgagg
     4501 attttgaata tgcctgcagt aacatacgaa cacattaaaa cttgtaaaca atcaggtgcg
     4561 cgtttaggta tcgtgcacac accacacggt tcatttgaaa cacctatgtt tatgccagtt
     4621 ggtactaaag caaccgttaa aacaatgagt ccagaagagt taagacaaat tgaagcaaaa
     4681 atcattttgg gcaacacata tcatttgtgg ttacaacccg gaaatgatat tatcaaacac
     4741 gctgggggat tacataaatt catgaattgg gatggtccga ttcttacaga ttcaggcggt
     4801 ttccaagtgt ttagtttaag taatttacgt aaaattacag aagaaggcgt ggaatttaga
     4861 catcatacta atgggtctaa attatttttg agtcctgaga aatcaatgca aattcaaaat
     4921 gatttaggat ctgatattat gatggcattt gatgaatgtc caccgatgcc tgctgaatat
     4981 gattatgtaa aaaaatctat tgaacgtaca acacgttggg cgaaaagatg tctagatgca
     5041 caccaaagac ctgaagatca agcattgttc ggcattatac aaggtggcga atatgaagat
     5101 ttaagagaac aaagtgcaaa ggatttagta gaattagatt ttcctggtta tgcaatcggc
     5161 ggtttgtcag ttggtgaacc taaaccggtt atgtataaaa tggttgaaca tacagagcag
     5221 tttatgccta aagataaacc aagatattta atgggtgtag gatctccaga tgcgttaatc
     5281 gaatgtagta ttcgcggcat ggatatgttt gattgtgtct taccgacacg tattgccaga
     5341 aatggtactt gtatgacatc gcaaggtcgt ttagttatta aaaatgcaaa atttgcagat
     5401 gatttaagac cgttagatga gaattgtgac tgttatacat gtcaaaacta ttcaagagcg
     5461 tatatacgtc atttaatcaa ggcagaggaa acttttggta ttcgtcttac tactattcat
     5521 aatttacatt ttctgctaaa attaatggaa gatataagac aagccattcg agaagatcgt
     5581 cttttagatt tcaaagaaga attcttcgag caatatggat taaatgttga gaacccaaaa
     5641 aacttttaag caagaggagc gtataaaatg caattttcat tactaatata tatagtcgta
     5701 atttttgcgg ttatgtattt cttgatgatc agaccacaac aaaaacgtgc gaaacagcat
     5761 cgtgagttga ttaataacat tcaatctggt caaagaatta caactattgg tggtattaaa
     5821 ggtactgtta aagcagtaga tgaaacaact gttgttatta cagttaatgg tcatggtact
     5881 gaattaactt tcgaaaaacc tgctattaaa caagttgacc cttcataatg agaagtggta
     5941 ttaaaaagga tgaaacgaat agcgtttcat cctttttaag ttataagtag tatgtagaat
     6001 agcttatttt tattccaaaa aaagaaattg tgatacataa taattgttaa atttaaatta
     6061 aatatatcta tgactatttt tactccctgc tctagtatgt taatatgtat aaggctaata
     6121 aattttgtaa gaaagattta acttgcgtta taaaatagta ttcaagtatt ctaaatacat
     6181 acgtaaataa tgaggtgttc atgtgaagaa aagtagtaga ataattgcgt tcttgctttt
     6241 agtcgtattg ttgtttgccg gaatggctgc aacttataaa agtgttataa agaatgtcaa
     6301 tttaggactt gatttgcaag gtggatttga ggtgctttat caagtcgatc ctttaaataa
     6361 aggcgacaaa atagataaaa aagccttaca atcaacagcg caaacattag aaaatcgtgt
     6421 taacgtttta ggtgtttcgg aacctaaaat tcaagttgaa gaacctaata gaattagagt
     6481 acaacttgct ggtgtaacag accagaatga agcacgtaaa attttatctt ctcaagctaa
     6541 tttaacaatt cgcgatgcag aagataaagt aaaattaagc ggttctgata ttaaacaggg
     6601 atctgccaaa caagaattta aacaggaaac aaaccagcct acagttactt ttaaagtaaa
     6661 agacaaaaac aaatttaaaa aagtaacgga agaaatctct aagaaacgtg ataatgtcat
     6721 ggttgtttgg ttagatttca aaaaaggtga cagctataaa aaagaagcac aaaagaaaaa
     6781 ccctaaattt atttctgcag caagtgttga tcaaccaatc aattctgata gcgtagaaat
     6841 ctcgggtgga tttaaaggac aagaaggcgt taaaaaagca aaacaaatcg ccgaattgtt
     6901 aaatgcaggt tctttaccag ttgacttgaa agaaatttat tcaaactcag taggtgccca
     6961 atttggtcaa gatgcattag ataaaactgt gtttgcatcg tttattggtg tagcattaat
     7021 ttatttattc atgcttggat tctaccgatt acctggttta gtagcgatta ttgcattgac
     7081 aacttatatc tatctaacgt tagtagcatt taatttcatt tccggggttc taactttacc
     7141 aggattagcg gcgttggtat taggtgtagg tatggctgta gatgccaata ttatcatgta
     7201 tgagcgtatt aaggatgaac ttcgaatagg tagaacgata aagcaagcct tttctaaagc
     7261 aaacaaaagt tcattcctaa caatttttga ttctaactta acaacagtta tcgccgcagc
     7321 agtattattc ttcttcggtg aaagttcagt taaaggtttc gcgacaatgt tattattagg
     7381 tattctaatg atctttgtta cagccgtgtt cttatcaaga ttcttattat cattacttgt
     7441 ttcatcaaat atattcaaaa atcaattttg gttatttggt gttaaaaaga ataaacgaca
     7501 tgatattaat gaaggtgtag atgttcatga ccttaaaact tcattcgaga aatggaattt
     7561 tgttaaatta gctaagccat taattggagt aagtatttta attgtagttg tcggtttagt
     7621 tattctttat atcttcaagt taaacttagg aattgatttc tcaagtggta cacgcgtaga
     7681 tttccaatct aaacaagcaa ttactcaaca aaaggtagag caagttgtaa aagatagtgg
     7741 acttaaagcg gatcaaattc agatcaatgg taaagacaat aaagtagcaa ctgttcaatt
     7801 taaagacgat ttaacgcgtg ctcaagataa taaattgagt gataatatta aatcgaaatt
     7861 cggtgacaca cctcaaatta acactgtatc accaattatt ggacaggaat tagcaaaaaa
     7921 tgcaatgctt gcattaatct atgcatcgat aggtatcatc atctatgtat cattacgatt
     7981 tgaatggcgc atgggtcttt catctgtatt ggcattatta catgatgtat tcattatagt
     8041 agcgattttc agtttattta gaattgaagt agatttaaca tttatcgccg ctgtattaac
     8101 aattgtcggt tattcaatta atgatacaat cgtaacgttt gaccgtgtac gtgaaaactt
     8161 acaaaaggtt aaagtgatta cgacaacaga acaaattgat gatatcgtta atagatcaat
     8221 tagacagaca atgacacgtt caattaatac agtattaaca gttattgtag tagtagttgc
     8281 tatactattc ttcggtgctc ctacgatatt caactttact ttagcattat ttatcggatt
     8341 gatttctggt gtattctctt caatcttcat tgccgttccg ctatggggaa taatgaaaaa
     8401 acgtcagttg aaaaaatcgc cgaaacacaa attagttgta tataaagaaa agaaatcgaa
     8461 cgatgaaaag attttagttt aaaatgaatt a
//
LOCUS       DAMQYT010000110         8380 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMQYT010000110 DAMQYT010000000
VERSION     DAMQYT010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8380
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.110"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            61..1590
                     /gene="aur"
                     /locus_tag="PGA33_001812"
     CDS             61..1590
                     /gene="aur"
                     /locus_tag="PGA33_001812"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HDG6473616.1"
                     /translation="MRKFSRYAFTSMAALTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            1957..2406
                     /locus_tag="PGA33_001813"
     CDS             1957..2406
                     /locus_tag="PGA33_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDG6473617.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            2409..2576
                     /locus_tag="PGA33_001814"
     CDS             2409..2576
                     /locus_tag="PGA33_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473618.1"
                     /translation="MYQMNNKNLFRITCTQVSYQILTCVRVFLWKERIHSHSIDCIKN
                     FIQHVFMGVFE"
     gene            2753..3988
                     /gene="arcA"
                     /locus_tag="PGA33_001815"
     CDS             2753..3988
                     /gene="arcA"
                     /locus_tag="PGA33_001815"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDG6473619.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            4021..5031
                     /gene="argF"
                     /locus_tag="PGA33_001816"
     CDS             4021..5031
                     /gene="argF"
                     /locus_tag="PGA33_001816"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDG6473620.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            5122..6552
                     /gene="arcD"
                     /locus_tag="PGA33_001817"
     CDS             5122..6552
                     /gene="arcD"
                     /locus_tag="PGA33_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDG6473621.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSVIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFSMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYTIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            6569..7510
                     /gene="arcC"
                     /locus_tag="PGA33_001818"
     CDS             6569..7510
                     /gene="arcC"
                     /locus_tag="PGA33_001818"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDG6473622.1"
                     /translation="MKEKIVIALGGNAIQTTEATAEAQQTAIRCAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFVEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            7609..8313
                     /locus_tag="PGA33_001819"
     CDS             7609..8313
                     /locus_tag="PGA33_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDG6473623.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLICHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
BASE COUNT         3060 a         1165 c         1628 g         2527 t
ORIGIN      
        1 gaaaaattaa attaatattt tatttataag ctgtttttaa gatttcagga ggaatgaaat
       61 gtgaggaaat tttcaagata tgcatttaca agtatggcag cattaacctt gttgagcact
      121 ttatcaccag cagcattagc gattgattca aaaaataaac cagctaattc tgatattaaa
      181 tttgaggtga ctcaaaagag tgatgcggtc aaagcattaa aagaattgcc taaatccgaa
      241 aatgtaaaaa atatttatca agattacgct gttactgatg taaaaactga taaaaaagga
      301 tttacgcatt atacattgca accgagtgtt gatggtgttc atgcacctga caaagaagtg
      361 aaagtacacg cagacaaatc aggaaaagtc gttttaatca atggggatac tgatgcgaag
      421 aaagtaaagc caacgaataa agtgacatta agtaaagatg acgcagccga caaagcattt
      481 aaagcagtta agattgataa gaataaagcg aaaaatctta aagataaagt cattaaagaa
      541 aacaaagttg aaatcgatgg tgacagtaat aaatacgttt ataatgttga gttaattaca
      601 gtgacaccag aaatttcaca ttggaaagtt aaaattgatg ctcaaactgg cgaaatttta
      661 gaaaaaatga acttagttaa agaagctgca gaaactggta aaggaaaagg tgtacttggc
      721 gatacaaaag atatcaatat caatagtatt gacggtggat ttagcctaga agatttaacg
      781 catcaaggta aattatcagc atttagcttt aatgatcaaa caggtcaagc aacattgatt
      841 actaatgaag atgaaaactt cgtaaaagat gagcaacgtg ctggcgtaga tgcaaattat
      901 tacgctaaac aaacatatga ttattacaaa gacacatttg gtcgtgaatc atatgacaac
      961 caaggtagtc caattgtttc attaacgcat gttaataact acggtggtca agataacaga
     1021 aataatgccg catggatcgg tgacaaaatg atctatggtg atggtgatgg tcgcacattc
     1081 acaagtttat cgggtgcaaa tgacgtagta gcacacgaat taacacacgg tgtgacacaa
     1141 gagacagcga acttagaata taaggaccag tcaggcgctc taaatgaaag cttttcagat
     1201 gtttttggat actttgtaga tgacgaggat ttcttaatgg gtgaagatgt ctacacacct
     1261 ggaaaagagg gagacgcttt acgcagcatg tcaaacccag aacaatttgg tcaaccagct
     1321 catatgaaag actatgtatt cactgaaaaa gataatggtg gcgtacatac gaattctgga
     1381 attccaaata aagcagctta taacgtgatt caagcaatag ggaaatctaa atcagaacaa
     1441 atttactacc gagcattaac ggaatactta acaagtaatt caaacttcaa agattgtaaa
     1501 gatgcattat accaagcggc taaagattta tatgacgagc aaacagctga acaggtgtat
     1561 gaagcatgga atgaagtagg cgtggagtaa aaatatataa acaagaagaa gtaatgttaa
     1621 acacttataa ataattaaat tttaaataca gcttatatat ggggtatacc tacattgata
     1681 attgactttg taggtatacc ctctctttgt aattagatga gaggagtgtg agggttgtct
     1741 gccgaaagac tactcggcag tctaaaatca ttacaagtag tagatatgtg ataattaaat
     1801 gctgacttag aatacaaaat tcattttaaa agttgtcaca aaaaatttac atgtattttt
     1861 attatatttt gcaaaacaaa gtgttaaatt ataaatgaaa catgcatgaa tttatttttt
     1921 aatacaagaa acgtaactac caaaggagtt tacaatatga agaaaagtaa acgattagaa
     1981 attgtttcta caatagttaa aaagcataag atttataaaa aagaacaaat catttcatat
     2041 attgaagaat attttggtgt aagatatagt gcaacaacaa ttgctaaaga cttgaaggaa
     2101 ctaaatatat atcgtgtccc tatcgattgt gagacatgga tttataaagc tattaataat
     2161 caaacagaac aagagatgag agaaaagttt agacactatt gtgaacatga agttctaagt
     2221 tcaatcatca atggttcata cattatcgtc aaaacctcac ctggtttcgc ccaaggcata
     2281 aactatttta tcgatcagct aaatatagaa gagatattag gtacggtgag tggaaatgac
     2341 actacattaa tcttaactgc ctcaaatgat atggcagaat acgtatatgc aaaattattt
     2401 aaatagacat gtatcaaatg aataataaaa atttgtttcg tatcacgtgt actcaagtta
     2461 gttaccaaat attaacttgt gtacgcgttt ttttatggaa agaaagaatt catagtcatt
     2521 caattgactg tataaaaaac tttatacaac atgtttttat gggtgttttt gaataaaaaa
     2581 tgtatatttt gacccaaaat gcctttattt atgtataaaa atccattatt atgtattgta
     2641 taacaaaaag atatgaaatt ttcgactttc tttatgtgaa tataatcaca tgtaagcgtt
     2701 tgaagattgt ctatactcta aatgaattca aagataaaag gaggaaatag acatgacaga
     2761 tggtccaatt aaagtaaata gcgaaattgg agctttaaaa actgtgttac ttaagcgtcc
     2821 tggaaaagaa ttagaaaatt tagtacctga ttatttagat ggattactat ttgatgatat
     2881 tccatattta gaagtagctc aaaaagagca tgaccatttt gcgcaggtgc taagagaaga
     2941 gggtgttgaa gtactttacc ttgagaagtt agcagctgaa agtattgaaa atcctcaagt
     3001 aagaagtgaa tttattgatg atgtattagc agagtctaaa aaaacaatat taggtcatga
     3061 agaagaaatt aaggcattat ttgcgacact ttctaatcaa gaacttgtag ataaaataat
     3121 gtcaggggta cgtaaggaag aaattaatcc gaaatgtaca catctagtag agtatatgga
     3181 tgataagtat ccattctatt tagatccaat gccaaacctt tattttacta gagatccaca
     3241 agcctcaata ggacacggta taacaatcaa tcggatgttc tggagagcac gacgacgaga
     3301 atcaatattt attcaatata ttgtaaagca tcatcctaga tttaaagatg cgaatattcc
     3361 aatctggtta gatcgagatt gcccattcaa tattgaaggc ggcgatgaac ttgttttatc
     3421 taaagatgtc ttggctatag gcgtttcaga acgtacatct gcacaagcta ttgaaaagtt
     3481 agcgcgacgt atttttgaaa atccgcaggc gacgtttaaa aaagtagtag caattgaaat
     3541 tccaactagt cgaactttta tgcacttaga tacagtattt acaatgatag attatgacaa
     3601 atttacaatg cattcagcca ttttaaaggc agaaggcaat atgaatatat ttattattga
     3661 atatgatgac gtaaataaag atattgccat caaacaatct agtcatttaa aagatacttt
     3721 agaagacgta ctaggtatag atgatatcca attcattcca acaggaaatg gtgatgtcat
     3781 tgatggtgct agagagcaat ggaatgatgg ctcaaataca ttatgtataa gaccaggcgt
     3841 tgtagtgact tacgatagaa actatgtatc gaatgattta ttgagacaaa aaggcattaa
     3901 agtcattgaa atatctggta gcgagttggt acgtggacgt gggggcccta gatgtatgag
     3961 tcaaccatta ttcagagaag acatttaacg taataaatta tagaaacgag ggtgaaaata
     4021 atgacagaaa ttcaaaaacc gtatgattta aaaggcagat cattattaaa agaaagtgat
     4081 tttaccaaag cagaattcga aggacttatt gattttgcaa ttacattaaa agagtataag
     4141 aaaaacggta ttaagcatca ctacttatct ggaaaaaata ttgcactact attcgaaaag
     4201 aattcgacga gaacgcgtgc tgcgtttaca gttgcgtcta ttgatttagg tgcgcatcca
     4261 gaatttttag gaaaaaatga tattcaatta ggcaaaaaag aatctgtaga ggatactgcg
     4321 aaagtattag gtagaatgtt cgatggtatt gaattccgtg gtttttcaca acaagctgtt
     4381 gaagatttag cgaagttctc tggtgtaccg gtgtggaatg gattaacaga cgattggcat
     4441 cctacacaaa tgttagctga ttttatgaca ataaaagaga attttggata tctagaagga
     4501 ataaacttaa cttacgttgg agatggacgt aataatattg cgcattcatt aatggtagca
     4561 ggtgctatgt taggtgttaa tgtaagaatt tgtacaccta aatcattaaa tccaaaagag
     4621 gcatatgttg atattgcaaa agaaaaagcg agtcaatatg gtggttcagt catgattacg
     4681 gataatattg cagaagcagt tgaaaataca gatgctatat atacagatgt ttgggtatcg
     4741 atgggtgaag aaagtgaatt tgaacaacgt attaatttat taaaagacta tcaagtgaat
     4801 caacagatgt ttgatttaac aggtaaagat tcaacgatat tcttacattg tttaccagca
     4861 ttccatgata caaatacact ttatggacaa gaaatttatg aaaaatatgg attagctgaa
     4921 atggaagtta cagaccaaat ctttagaagt gaacattcaa aagtgtttga tcaagctgaa
     4981 aatagaatgc atacaattaa ggcagtaatg gcagcaacat tggggagtta atcactaaat
     5041 ggaacgatat gaatatgatg tgtctgatga tataagtgtc atgtacagac acctcatatt
     5101 ggtattaaag gagaaatgaa tatgaacgaa tcaggagata acaaactcag taaatcttct
     5161 ttaattggac tagttatagg atccatgatt ggtggcggtg cgttcaatat aatgtctgat
     5221 atgggcggta aagccggtgg attagccatt attattggtt ggattattac agctatagga
     5281 atgatttcat tagcgttcgt atttcaaaat ttaaccaatg aacggccgga gctagacggt
     5341 ggtatttata gttatgctca agcaggattt ggcgattttg taggatttat cagtgcttgg
     5401 ggatattggt tctcagcgtt tttaggcaat gttgcctatg caacactatt gatgtcagca
     5461 gtaggtaact ttttcccgat ttttaaagga ggcaacacat taccaagtgt tattgtcgcc
     5521 tcgttactac tctggggtgt ccatttcttg attttaaaag gcgttgaaac agcagcattt
     5581 atcaatagta ttgttactgt tgcaaagtta ataccgattt tacttgtaat catatgcatg
     5641 ataattgcat tcaattttga cacttttaaa acaggctttt tcagtatgac gtcagagggt
     5701 gtattgccat ttagttgggc gagcacaatg agccaagtta aaagtacgat gctagtgaca
     5761 gtttgggtgt ttatcggtat cgaaggtgca gtaatttttt ctagtagagc taaaaataaa
     5821 aaagatgtag gtagtgccac ggttatagga cttatatcag ttttaattat ctatttctta
     5881 ttaactgtat tagctcaagg cgtgattttg caaaatcata tttcgcaatt agattcgcca
     5941 agtatggcac aggtgcttgc aactattgta ggtggttggg gatctacact tgtaaatatt
     6001 ggtttaatta tttcggtact aggtgcatgg ttaggatgga cactgcttgc tggtgaatta
     6061 cctttcattg ttgcaaaaga tggattattt ccaaaatggt ttgctaaaga aaataaaaat
     6121 ggagcacctg taaatgcact gcttattacc aatatattag tacaattatt tttaataagt
     6181 atgctattta cacagagtgc gtatcaattt gcattttcac tagcatcaag tgctatttta
     6241 tacccttaca tgttcagtgc attttaccaa gttaaataca ctttagagca tcgacagcaa
     6301 gcaactacta aacaatggac gattggtatc atagcctcaa tttatgctat atggcttata
     6361 tatgcagcag gtatcaatta cttattattg acgatgttac tttatattcc agctcttctt
     6421 gtttatacaa tcgttcaaaa gaataatcag acacgtttga ttaaatcaga ctatattctt
     6481 tttatgatta ttatcgtact tgcagttatc gggttaatta agttattgat gggaacgata
     6541 aatgtttttt aaaaggagcg acaaaaatat gaaagagaaa attgtcattg cattaggcgg
     6601 taatgcgata cagacaacag aagcaacagc tgaagcacaa caaacagcta ttagatgtgc
     6661 gatgcaaaac cttaaacctt tatttgattc accagcgcgt attgtcattt cacatggtaa
     6721 tggtccacaa attggaagtt tattaatcca acaagctaaa tcgaacagtg acacaacgcc
     6781 ggcaatgcca ttggatactt gtggtgcaat gtcacagggt atgataggct attggttgga
     6841 aactgaaatc aatcgcattt taactgaaat gaatagtgat agaactgtag gcacaatcgt
     6901 tacacgtgtg gaagtagata aagatgatcc acgatttgat aacccaacta aaccaattgg
     6961 tcctttttat acgaaagaag aagttgaaga attacaaaaa gaacagccag actcagtctt
     7021 taaagaagat gcaggacgtg gttatagaaa agtagttgcg tcaccactac ctcaatctat
     7081 actagaacac cagttaattc gaactttagc agacggtaaa aatattgtca ttgcatgcgg
     7141 tggtggcggt attccagtta taaaaaaaga aaatacctat gaaggtgttg aagcggttat
     7201 agataaagat tttgctagtg agaaattagc aacgctgatt gaagcagata ccttaatgat
     7261 tcttacgaat gtagaaaatg tatttattaa ctttaatgaa cctaatcaac aacaaatcga
     7321 tgatattgat gtagcaacac tgaaaaaata cgcggcacaa ggtaagtttg tggaaggatc
     7381 gatgttgcca aaaatagaag ctgcgatacg atttgttgaa agtggggaaa acaaaaaagt
     7441 tatcattacc aatttagagc aggcatacga agctttgatt ggtaataaag gtacacacat
     7501 tcacatgtag cactttatca cgcgacaaaa cattaaatat gtttctccgt tgattcaaat
     7561 gaaaaagttg tctgctgaca ctttgcaagg tttgaaggag tttaacttat gacagaaaac
     7621 tttattttgg gtagaaataa taaattagaa catgaactaa aggcattagc agattacatt
     7681 aatataccat atagtatatt acaaccatat caaagtgaat gttttgtcag acattatacg
     7741 aaaggccaag ttatttattt ttcgccacaa gaaagtagca atatttactt tttaattgaa
     7801 ggtaacatta ttagagaaca ttacaatcaa aatggagatg tatatcgtta ttttaataaa
     7861 gagcaagtat tatttccaat cagtaactta tttcatccga aagaggttaa cgaattgtgt
     7921 acagcattaa ccgattgtac agttcttgga ttgcctagag aattgatggc ctttttgtgc
     7981 aaagctaatg atgatatatt tttgacactt tttgcattaa taaatgataa tgagcagcaa
     8041 cacatgaact ataacatggc attaacaagt aaatttgcta aagatcgaat tatcaaattg
     8101 atatgccatc tatgtcagac agtaggatac gatcaagatg aattttatga aatcaaacag
     8161 tttttaacta ttcaactcat gagtgatatg gctgggattt cccgggaaac agctggtcat
     8221 attattcatg aacttaaaga tgaaaaactt gttgttaaag atcataaaaa ttggttagta
     8281 agcaaacatt tattcaatga tgtatgtgtt taatatacaa tgtaaaatga ataagttgaa
     8341 catgaggtct aacgtacatt tatacgttag gccttttttg
//
LOCUS       DAMQYT010000111         8358 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMQYT010000111 DAMQYT010000000
VERSION     DAMQYT010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.111"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            195..635
                     /locus_tag="PGA33_001820"
     CDS             195..635
                     /locus_tag="PGA33_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HDG6473624.1"
                     /translation="MNTKYFLAAGAVITTLALGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            977..1450
                     /locus_tag="PGA33_001821"
     CDS             977..1450
                     /locus_tag="PGA33_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDG6473625.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            1425..2111
                     /locus_tag="PGA33_001822"
     CDS             1425..2111
                     /locus_tag="PGA33_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDG6473626.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            2111..3166
                     /gene="saeS"
                     /locus_tag="PGA33_001823"
     CDS             2111..3166
                     /gene="saeS"
                     /locus_tag="PGA33_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HDG6473627.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCNEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENEDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(3238..4221)
                     /gene="csbB"
                     /locus_tag="PGA33_001824"
     CDS             complement(3238..4221)
                     /gene="csbB"
                     /locus_tag="PGA33_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HDG6473628.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSLENDEKDTHKVYSK"
     gene            complement(4353..5192)
                     /locus_tag="PGA33_001825"
     CDS             complement(4353..5192)
                     /locus_tag="PGA33_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDG6473629.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            complement(5414..6763)
                     /locus_tag="PGA33_001826"
     CDS             complement(5414..6763)
                     /locus_tag="PGA33_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDG6473630.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            complement(6984..8165)
                     /gene="nagA"
                     /locus_tag="PGA33_001827"
     CDS             complement(6984..8165)
                     /gene="nagA"
                     /locus_tag="PGA33_001827"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDG6473631.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEVDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDNALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
BASE COUNT         2850 a         1488 c         1140 g         2880 t
ORIGIN      
        1 ccttacttaa aaaaacgccc tcctcttatt ttgaccccta tttatttaaa tcagacaatt
       61 attttcattt tcaaattatt ctttcttcaa tattagttaa gcgatattta aacgaagtta
      121 agaattagtt aatggcatat tatttgcctt cattttaaac ttaacttatc aaattgaaga
      181 aatgaggagt tagcatgaat acaaaatatt ttttagcagc tggtgctgtt atcacaacat
      241 tagctttagg tgcttgtggt aattctaatt cacaagatca aggtaacaaa actgaacaaa
      301 aaacaaagtc agaagatagc aatgttaaaa ctgataaaac aaaacaccta acaggtacat
      361 tcagttctaa aaacggtgaa actgttgaag gtaaagctga gattaaaaat ggtaaattaa
      421 tgcttactaa ctacaaatca tcaaaaggtc cagatttata cgtctaccta acaaaaaatg
      481 gcgacattaa aaacggtaaa gaaatcgcaa tggttgacta cgataaagaa aaacaaacat
      541 ttgatcttaa aaatgtagat ttaagcaaat atgatgaagt aacaatctat tgtaaaaaag
      601 ctcacgtcat cttcggcggc gctaaattaa aataatcaat ttctgagtta aacttttatt
      661 tacaacatac tattactata cattacaaat ttttaaaata tacattacac tcattactca
      721 atggaaagcg tatgatttca cagcccccct agcttgtaga aatcatactt tccttttttc
      781 aatatatata caactattaa atcccataag attgcagagc acataagtaa atttttttag
      841 agcttgaggt ttgtttagct taagcaaccc atgagctcaa acacttcctg ttcacataac
      901 actacaaatc gcattatgtt gcttaatctt atgtttatat aaattacaca caataaatag
      961 aaagaatgtg aacatcatga ataaattatt gctactcgtt acatttatca ttcgtgtggg
     1021 ttcaggtatt gttatgttaa tgcaaggcta cgaaaaatta acgggcggat ttacgctgaa
     1081 aggtttagta ccagtcatcg ctaacaatac tgattcacca gagtggtata agtggttttt
     1141 cgcaaatata gttgcacata cgacgtcatt atttgatatt gttgtcccac tcggagagat
     1201 tgcaattgga ttaggtttaa tttttggagt ttttgcatat gctgctagtt tctttggagc
     1261 ctttgttatg ataaattata tcttagcaga tatgatattt acgtatcctc ttcaattaac
     1321 tttctttatc cttttactaa tgagtcactc attgttaaaa cagatttcac ttaaagaaat
     1381 cattaattac tttagaggtc gtaagaacag aggtgaaaaa atagatgacc cacttactga
     1441 tcgtggatga tgaacaagac attgtagaca tttgtcaaac ctattttgaa tatgaaggtt
     1501 acaaagtaac aacgacaact agcggtaaag aagcaatttc tttactatca aatgatattg
     1561 atatcatggt acttgatatc atgatgccag aagttaatgg ttacgacatt gtcaaagaaa
     1621 tgaaaaggca aaaattagat atccccttta tctatttaac tgccaaaaca caagaacatg
     1681 ataccattta cgccttaact ttaggtgcag atgactatgt caaaaaacca tttagtccaa
     1741 gggaactcgt tttacgtatt aataatttac ttacaagaat gaagaaatac catcatcaac
     1801 cagttgaaca actgtcgttt gatgaattaa cacttattaa cttaagtaaa gttgtgactg
     1861 taaatggtca cgaagtccct atgcgtatta aggaatttga gttattgtgg tatttagctt
     1921 ctagagaaaa tgaagttatt tctaaatcag aattacttga aaaagtttgg ggatatgact
     1981 attacgaaga tgctaatacc gtgaatgtcc atatacaccg tattagagaa aaattagaaa
     2041 aagagagctt tacaacatat accatcacaa ctgtatgggg attaggatat aaatttgaaa
     2101 ggagccgata atggtgttat caattagaag tcaaatcatt attggcgtcg tatcgagtat
     2161 actattaact tcaactattt tagcaattgc atatatttta atgtggttta acggccatat
     2221 gacactaact ttgaccttaa cgacaataat tacaagctgt ttaaccttat taatatgtag
     2281 tatttttatt aatccactta tacaaaaaat taagcagttt aatataaaaa ctaagcaatt
     2341 tgctaacgga aattacgcaa gcaatgataa aacgtttaat tcaccaaaag aaatttatga
     2401 attaaatcaa tcttttaata aaatggcttc tgaaattacg caacaaatga atcaaattaa
     2461 atccgaacaa caagaaaaaa cagaactgat tcaaaactta gcccatgatt taaaaacacc
     2521 tttagcaagc attatttcat attctgaagg actacgtgat ggtataatca ctaaggatca
     2581 tgagattaaa gagtcatacg acatattaat taaacaagca aacagattat caacattatt
     2641 tgatgatatg actcatatta tcactttaaa tacaggtaaa acatatcccc cagaattaat
     2701 acaactagac caattacttg tatcaatatt gcaaccatat gagcaacgta tcaaacatga
     2761 aaaccgcaca ttagaagtga atttctgtaa cgaaattgat gcattttatc aatatcgaac
     2821 gccacttgag cgtattttaa caaacttact tgataatgcg ctaaaatttt caaatgttgg
     2881 tagtcgcatt gatattaata ttagtgaaaa cgaagatcaa gatactatcg acattgctat
     2941 tagcgatgaa ggtattggca ttataccaga actacaagaa cgtatattcg aacgtacatt
     3001 cagagtagaa aactctcgta atacaaaaac gggtggttct ggattaggct tatatatagc
     3061 taatgaactc gcgcaacaaa ataacgcaaa aatcagtgta agcagtgata tagatgtagg
     3121 aactacgatg actgtaacat tacacaaatt agacattacg tcataatccg atttatttat
     3181 aaaataaaat gcaaagacta aaaagaagct cccattaatg agggcttctt tttttgttta
     3241 tttagaataa actttatggg tatccttctc atcattttca agacttgaaa gatttgtagc
     3301 ttgaataata tatttaggac gtgccttaac ttcataatat atcctgccaa tatattcacc
     3361 tacaacacca attgaaatta actgtatgcc gcctaataat aaaatagctg caatcgttga
     3421 aaaatatccc ggaatattaa caccagatat cataatattg atgaataaat agataatata
     3481 taacacgctt attgaaaaga taaacaagcc aagataaatc atcattctca aaggtttact
     3541 attaaaggaa atcaatccat caataccata attaaataac tttttaaagg accacttaga
     3601 ttcccctttt tgtctctcaa cattttgata cgtaaacact ttagtattat agcctatcca
     3661 ttcaaataac ccttttgaaa atcgattata ttcttcaagt gatgcaatgg atttaacagc
     3721 tctttggctt aaaagtctaa aatcaccaac accatcatca aattgtactt cttctacaaa
     3781 gcaattaact aacttataat acaaatggct taatgttttg cgactaaaat tttcaccact
     3841 tctatcacgc tttgcaatca cttgatcata gccttccata aaaccttcaa ccattttcgg
     3901 aatatattca ggtggatgtt gcaaatcaca atctatcatg atgactgcat caaattcagt
     3961 actatgctgg taacctgcaa tcatagctgc ttcttttcca aaatttctac taaaagaaat
     4021 aaagctgaca tgcctatcta ttgtggcaag atgttgcatt tcatctatag tggtatccgt
     4081 actaccatca tctatgaaaa gcatattata atcatagcct ttcacactac tatcttgtga
     4141 aagtatttca gttaattgtt gatgtgtttg tgtaatgact tccccttcat taaaacaagg
     4201 aatgacgact ctaattttca tctatgtcca tcctttttat ttaaaagatt ttgaatgtac
     4261 ctgagtattg ttttatcaaa tagtaaaaac caactcattt attttaatgt acacattaaa
     4321 aggcattcat aaacgtaaaa tttaacatcc agttatttta aatccccaat tttcacatca
     4381 tctggatttt taccttgtct tgcatttcta tttaaagcat caatttcagc tacttctgtt
     4441 aattctaaat taaaatctaa aatatctata ttttcttgaa tgcgtttagg tgtctgagat
     4501 tttggaataa taattctatt gtgtgctaaa tgccaacgta atacaacttg tgctggtgtt
     4561 ttatgatatt tttcagcaat tttaacaatg acagggtcgt ctagtagtcc tctatttctc
     4621 atcaaaggca tccatgcagt cactttaata tcgtgacgat cacaaaattc ttgtaattct
     4681 tgttggttaa aatatggatg tacctcaatt tgattcacca ttggtttgat acttgattga
     4741 gccattaatt tttctagatg atgaacatta aaattacata cacctattgc ttttacctta
     4801 ccttgctcgt aaagttcttc catagcttta tatgtttcta aaaatagacc atctgcttca
     4861 caaggccaat gtattagaaa taaatcaaga taatcagttt gtaaattttc aatcgatttg
     4921 ttgaaatatt cgaatgtttt ctcataacct tgatagtcat tccataactt cgttgttata
     4981 aacaaatctt ctctatcgac gccattatcc tttaatgctc gtcctagtga agcctcatta
     5041 tcataaaagt atgctgtatc aaacgctcta tagcctgcgt caattgcagc atttacaact
     5101 ttagtcatat cttcgtcaga gattttataa acacctaacc caactgaagg catcgggtat
     5161 ccattattta atatttgtat ctcattcaac atctttatat ctctccaatc tatgtatctt
     5221 ttatatcttt acattaccct aaatttttca acaaactcaa ttaatacgaa ttatcgcttt
     5281 caataaaaat tattcattaa atcattaaag atattgagtt ccaatactat tttcactttt
     5341 cattaatttt agtcaaaaaa ataaccaacc aaaaatgaat taaatcattc ttagttggtt
     5401 tatatattaa tatctattct gatttttcat cttcatcaga ctgtccgata gtaggtctcg
     5461 cttcattaaa ttcatagttt aatatcaccc aaataatttg gtggttatcg atttctgaaa
     5521 caacccagcg atcataagtt gtatccacgt aatcatcttt ttgtaaattg gtattacgag
     5581 attgtaacca tccacctatc gtatcaatat cctcagagtc atcaaattct ataccgaact
     5641 cttcagttaa atcatccaat agtactctgc catttacttg gaatgtctta ttatcaattt
     5701 taacgatatc attcacttca tcatcatcaa attcatcacg aatttctcca acgatttctt
     5761 ctaaaatatc ttccatcgtt aaaatacctg ccgttccacc atattcatct ataataagac
     5821 tcatatgtac atgttcacgt tgcattctaa ttaatgcatc actgatacgt gttgtctctg
     5881 aaatcattgg caactcatgt atatagtttg ctattttaat cgtttttcca gaagcgtatt
     5941 cagttaaaaa ttctttgacg ttaataaatc ctttaatgtg gtctttatca ccatcatcag
     6001 taattggata acgcgtaaat tgatgttctt ttattgtttc tagtaattcg tctacattaa
     6061 aaggttcatt tagtgtaatc atttgagttc taggtaccat tatatctttt gcatgtcttt
     6121 catcgaatga aaagatattt tgcatatatg ccaattcagt ttggttgatt tctccaccat
     6181 tataactatt gttaataata attttgattt cttcttctga cattgcatca gtttgggcat
     6241 caggatttac accaaacatt ctaataataa cacgtgcaga accattcatc agccaaatca
     6301 atggtttcat aatgttaccg aaatagaaca atggtcttgc atatactaaa gcaagctttt
     6361 cagtatgttg aatagctata gatttaggcg ctaattcacc aagtactaca tgcaaatacg
     6421 taacgattat aaatgacact gcaaacgaaa tcgtcgtcgt taatgcagtt ggtaaattga
     6481 ttgcttcaaa tattgggtgt aatagctttt caaacgttgg ttcaccaagc caacctaacc
     6541 ctaaagatgt tactgttata cctaactgac aagcagaaag ataataatct agattagcaa
     6601 tcatcttttt tactatttta gcaggtttat ttccttcatc tgctagctgt tcaattcttg
     6661 ttgctctaat ttttactaat gcaaattctg aaccaacaaa tacagtggtt aatgcaatta
     6721 atagaataaa tataatcaaa ctaattatgg tcgaagtttc caattaattc cctatttcta
     6781 gggattcacc tccatgttat atgtgtcact catgggtaac acgcattcaa atttatcact
     6841 atgacttaaa ttacaactac atgttatcgc cttcccattt agacaccccc agaaaaaaat
     6901 gttattgctt ctattttatc ataatataaa gtgcttatgt taacagatta aatctattgc
     6961 atacatttaa ttatgattat ttattagcta aatgtgtgaa ccttgccttg ttttattgta
     7021 gattttacat tcatatcatc atctagaata acaagatctg catccttatt tactttaata
     7081 ctaccttttc tatcatcgat acctaatgca atggcttgat ttaaacttgt tactcgccat
     7141 aaatgatcta atgtatcacc tgtaaatgat attaagttac gtaacccatg attcattttt
     7201 aaaatactac cagcaagcgc accatttgca agacgtgctt gttgcgattg aacagttact
     7261 ttttgtccac ccaaatcata ttctccttca ggcatacctt ttgcacgcat tgcatcggta
     7321 attaaataaa aacgttcatt acctttcata cggtaagcaa ttgcaaccga tgccggatga
     7381 gaatgagtgc catcaacaat catttcggta tgtagagcat cattcaacca tgctgctcca
     7441 aaaacacctg gttctctatg ttggaatggc gtcgctgcat tatataaatg cgtgacatgt
     7501 ttagctcctc gctcaacagc ttcaactgct tcttcgtatg ttgctactgt atgaccaatt
     7561 gaaaaaataa tgtcatcttt atacgtttca agcgcttctt ttgcaccttc aacttcaggt
     7621 gcaaacgtca taatctttat taatccgtta gcagtctctt gaaaatgttt aattttatcg
     7681 ataaatgggc gtacaacgta ttgcggatgt tgagcaccaa ctttattttc agatataaat
     7741 ggtccttcta aatgtatacc tacaatttcc gctgcattgt gaacatcttg ctccgcttca
     7801 tatttagcaa tatttgtaag tgcattatct attttatcag tcgattgcgt cattgtagtg
     7861 gccaagtatg atgtcgtccc ttcagacaac aaattttcgg atagatattt taagccatcg
     7921 tatgacccat ccattgcatc ttgaccataa ccaccatgaa tatgtatatc aataaaacct
     7981 ggtaatacat gatgaccttt agcatcaatc acttgaattt tatttgtcgt atcattatca
     8041 attgctgctt tatcatccac ttctccaatt gcaacaatct gtccatcttt cacatgaatg
     8101 taaccattat cgattttgcc atcttcagta taaactttgc cgttatatat aattaattct
     8161 gacacgtata aacccttctt tatttataaa ttttcacttt aagacactca tattttacta
     8221 tatctgcttc gtttggcata actttttgca aaatattaaa aaagtctata catttgtggt
     8281 gtctatttct tcattcattt atctaaaaat aaaaacactt tcatatcaat tcataaaaac
     8341 tgaattaata gaaagtgt
//
LOCUS       DAMQYT010000112         8042 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMQYT010000112 DAMQYT010000000
VERSION     DAMQYT010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.112"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            204..389
                     /locus_tag="PGA33_001828"
     CDS             204..389
                     /locus_tag="PGA33_001828"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473632.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
     gene            complement(612..1190)
                     /locus_tag="PGA33_001829"
     CDS             complement(612..1190)
                     /locus_tag="PGA33_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDG6473633.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(1454..1834)
                     /gene="blaI"
                     /locus_tag="PGA33_001830"
     CDS             complement(1454..1834)
                     /gene="blaI"
                     /locus_tag="PGA33_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HDG6473634.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(1824..3581)
                     /gene="blaR1"
                     /locus_tag="PGA33_001831"
     CDS             complement(1824..3581)
                     /gene="blaR1"
                     /locus_tag="PGA33_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HDG6473635.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            3688..4533
                     /gene="blaZ"
                     /locus_tag="PGA33_001832"
     CDS             3688..4533
                     /gene="blaZ"
                     /locus_tag="PGA33_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HDG6473636.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            complement(5059..5478)
                     /locus_tag="PGA33_001833"
     CDS             complement(5059..5478)
                     /locus_tag="PGA33_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDG6473637.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            5602..6573
                     /locus_tag="PGA33_001834"
     CDS             5602..6573
                     /locus_tag="PGA33_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDG6473638.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            complement(6745..6837)
                     /locus_tag="PGA33_001835"
     CDS             complement(6745..6837)
                     /locus_tag="PGA33_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDG6473639.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(6994..7341)
                     /locus_tag="PGA33_001836"
     CDS             complement(6994..7341)
                     /locus_tag="PGA33_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDG6473640.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNHEGF"
     gene            complement(7360..7977)
                     /gene="cadD"
                     /locus_tag="PGA33_001837"
     CDS             complement(7360..7977)
                     /gene="cadD"
                     /locus_tag="PGA33_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HDG6473641.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
BASE COUNT         2863 a         1160 c         1055 g         2964 t
ORIGIN      
        1 atttactata tgggcatctc tttcattatt aatacaaagc tttgtatctc cgatattagg
       61 caaaggtgta aacgaattag gtgatactat aggattcaac attatattaa tcgtttcttt
      121 aataatgatt atcttcttat tttttataaa tcgtagtaaa aatatctcct atgattatat
      181 aaaagttcaa agaggtgaag ccaatgaata aattagaaaa atggtctttg gtatttatta
      241 tttttacatt tctctcttca ttagcacttt tatatttcaa atataatttt agttcggtta
      301 tgccagtcct aagtctttct attttaacta tactaatatt gttgacgagt attattactg
      361 tagtttcagt taatgatgaa gatacttaaa acgttcatta gtgaaacagg tattatatta
      421 aaaaagcgta cgaatttgta cgcttttttc ttgtgttatt attaaaatct cataacagga
      481 aaatattatg agattttaga cgctgtcaga acgtctcttt tacatcgagt gacattgaat
      541 caaagtcttt gtatgttaat gatatataaa attatttagt agattcatta tagtaagcta
      601 gattagttgc tctacttgtt tttattctct tttgcctcta atttattgag ataacgataa
      661 atagttgtac gtgaaacttg ccactgctct gcaattgttt taattggcgt accattatca
      721 taaagtgtct taagtaagtt taaatcttgt ttatttaatt tttcaggtct acctccatat
      781 cggcctctag cacgtgctgc aattcgaccg gctgatgatc gctctaaaat taaattacgt
      841 tcaaactctg caaatgctgc gaataaatga aataataatt gtcctgtaga acttgattta
      901 tccatcgtta tattttcttc taaactatga aaactcacac ctcgttcgtt aagttcatta
      961 actaacgtga ttaaatcctc catgttccgt cctagtctat ctaatctcca aacaacaatc
     1021 gtatctcctg acctcgcaaa ttcaattgct ttatctaaac caggtcgctt actttttgaa
     1081 ccactcatat ggtcattaaa tatcttttca caaccatatg tatttaatcg atcctcctgt
     1141 aaattcaaat tttgtaatcc agttgaaact cttgcataac ctattttcaa aatacagacc
     1201 tcctatatac acctttagtg taccataact tgattacgag gtcttttgtg aacatagatt
     1261 ttttgttggg tttttgaaca cataattcta tgtaatactt acttatttag acaaaaaaca
     1321 agagtgtaca gaaaatggtt attttctgga cactctcatc tttaataata tattttaaat
     1381 gtcattaaca aaactaattt aataagagtc aagtatggtt tacatatgta ttatgtttga
     1441 aacatacctc taattacttt ttactaatat catttaaaat gtctcgcaat tcttcaattt
     1501 ctttgttatt taattcttca tttttcgcaa aattcagcac taaacttttc atatcccctc
     1561 catacagttt attaagaaag gttttagcag ttttcatttt aatatcgtct tctttaatat
     1621 ttgatgagta aaaataaata ttttctgatt tgtatcgttt tataatctct tttttatata
     1681 gtcttgtgat taatgttcta atcgttttat cactaacttc tttatatttt tgaatttcaa
     1741 ctacaatttc attagctgat actgattttt tatcccatat tatattcata acatcccatt
     1801 cagccataga tatttcaact tgcttattgg tcatttaaaa cacccattcc ttttaatatt
     1861 ttttcactta ttaattcagc atttttccca gacgggtttc catctgataa atgtgtagca
     1921 aaataatact tatcatgatt tgtaattacg taacctacaa accacccatt attatacttc
     1981 ccgtttacta tacctgtacc tgttttccca tacagttcat acttttcatt tttcttaatc
     2041 aataatgaag aagataattg attctttgct tttttactaa aatggttatt ttgttccatc
     2101 atatttttaa aaactattac ttgttcaaga ttagatattt tcaaactatc ttccatccaa
     2161 tagcttttat aacttcccaa attttcatta ccataattta attgcttgag ttgagccgca
     2221 gtatagttct ttggtatttg attactaata cgttcgaagt accaattaac tgaattttgc
     2281 attgctgtat ttaaatcttg ttccttattc caagactcga aaagataatg tttatgattc
     2341 catgacatac gtgaattttt atcacttata atatgacggt caagtccaaa cagtgctaaa
     2401 taaattttgt aagttgaatc aggagaatat ctttttctac tttctttttc attataaata
     2461 tagtatttgt ctttcttcat gctgtacata acgaaagatc cactatttga accaaaattt
     2521 ttactttcat ctaaaatttg gtaatcgctt tgtaagggct ttttataatt ataatcagtt
     2581 aaggattgcc ccattaaaaa ctggctttga attatcatga taaaaaaagt aaaaatacaa
     2641 ataaaaatta gaattaattt tgactgcttt ttcagatcgg cttcttttat attaattaat
     2701 ctacttttga gtaatgattt tttaccatta aatgaatggc ttaatatatt tttgttggaa
     2761 aatggggttt ttaatacgga gtccataata gattccgcat aagttttaaa ttcatttttg
     2821 ttaatattat taaggacaaa tctatcggct tctacttcat tgtcatgaat tatctttctt
     2881 tttacaatat gtactaatgg attgtaactc ataataatag agaagatatt aaagataatt
     2941 aaatgtaaag tatctctatt tttagcatga gcatattcat gcagtattat atattttagt
     3001 cttttatcaa ttacactttt aaaatatgaa ctaggaatca aaataatata tttcccatac
     3061 caaaaagtta ttggagattg aatagcctct gcttttcgaa tcacaatatt ttttttatat
     3121 tgatggttga aaagtatcgt atctatttta tttttttcat tttcgtttag ataaagtgac
     3181 tgtttcttta aatatttaag atataataag gctttcaaaa atttaaaact taaaataata
     3241 actaaaacta tccaaataac tgtgcagata ttatcaattg aatcccaatt aaacttatgg
     3301 atatctgttg tgaactcttg aataggtttg gtggtattta tgttatggtt caaatcgtgt
     3361 gacttacttt caactgtggg cgcttgatta ttcacattat taaattttat aaaagagaat
     3421 ttaattggga taaaaggaat taatccagca agcatagtta gataccaaac tttataattt
     3481 aaactataat taaaatagcg ttttaatatg tacctaaaaa atactaacaa tagaaatata
     3541 aaacaaaagc ttactacgct cattattagt aatttagcca ttttgacacc ttctttcaaa
     3601 tatttataat aaacaattga catcaatatt acaattgtaa tattattgat ttataaaaat
     3661 tacaactgta atatcggagg gtttattttg aaaaagttaa tatttttaat tgtaattgct
     3721 ttagttttaa gtgcatgtaa ttcaaacagt tcacatgcca aagagttaaa tgatttagaa
     3781 aaaaaatata atgctcatat tggtgtttat gctttaaata ctaaaagtgg taaggaagta
     3841 aaatttaatt cagataagag atttgcctat gcttcgactt caaaagcgat aaatagtgct
     3901 attttgttag aacaagtacc ttataataag ttaaataaaa aagtacatat taacaaagat
     3961 gatatagttg cttattctcc tattttagaa aaatatgtag gaaaagatat cactttaaaa
     4021 gaacttattg aggcttcaat gacatatagt gataatacag caaacaataa aattataaaa
     4081 gaaatcggtg gaatcaaaaa agttaaacaa cgtctaaaag aactaggaga taaagtaaca
     4141 aatccagtta gatatgagat agaattaaat tactattcac caaagagcaa aaaagatact
     4201 tcaacgcctg ctgctttcgg caagacttta aataaactta tcgcaaatgg aaaattaagc
     4261 aaaaaaaata aaaatttctt acttgattta atgtttaata ataaaaacgg agacacttta
     4321 attaaagatg gtgttccaaa agactataag gttgctgata aaagtggtca agcaataaca
     4381 tatgcttcta gaaatgatgt tgcttttgtt tatcctaagg gccaatctga acctattgtt
     4441 ttagtcattt ttacgaataa agacaataaa agtgataagc caaatgataa gttgataagt
     4501 gaaaccgcca agagtgtaat gaaggaattt taatattcta aatgcataat aaatactgat
     4561 aacatcttat agtttgtatt atattttgta ttatcgttga catgtataat tttgatatca
     4621 aaaactgatt ttccctttat tattttcgag atttattttc ttaattctct ttaacaaact
     4681 agaaatattg tatatacaaa aaatcataaa taatagatga atagtttaat tataggtgtt
     4741 catcaatcga aaaagcaacg tatcttattt aaagtgcgtt gcttttttct catttataag
     4801 gttaaataat tctcatatat caagcaaagt gacaatagca ttttacaagt attttttatt
     4861 gttcgattag ggtaaaccta aataatacat aaattatatt acaatatgtt tattatagag
     4921 gctaaagtat cttagaaatt tggatagtag gtttttcggg aataaaagta tagttggctt
     4981 taatatataa ttaagctaag ctatcattta tatttactcg attataattc gcacttcaac
     5041 ttttttataa cgtaaaaatt aaaacgtagt tttatttatc tttagtaata tctttttaaa
     5101 cactacagtt tcttcattag ttaaattagc gaagtaatga ttattaagtt gataggcttt
     5161 cttttctaat gttggccata agtcttttgc tttatttgta ctgtttatta gcacagttct
     5221 tctattttca ttatcagttt gacgtgttat tagatccttt ttggacatct tatctaatat
     5281 tccagatatt gttgaaggtt ctaaatttaa agtattacca atcgctttag gtgtattcaa
     5341 attactatta atcaagcaat tcaaaacacc atattctgct ggagtaatat tatagggact
     5401 taataaactt ttaaaatgtt taaaaactct attctgagaa gtactcaaca aataatttat
     5461 acaattatct aattccacaa ctaaaacctc tcttaaatca ttagtattga ctcgcttatc
     5521 atatcatgat aagttttgta ggaaaatagt tttgcaacaa aaacatactt ctacaaaaca
     5581 attgtgaaag aaggctaata aatgttcaat gcggtagtag tgagaaaaag tgcatcaaat
     5641 gttagttata aagtagaaaa agttgatgaa tctcatttat cgaagggtga tgtattagta
     5701 aaagtggtat actcttcaat aaattataag gatatgctag ctttacaata taaaggcggt
     5761 gtaattagag attatcctat gattcctggt atagattttg caggtattgt tgaatcaagt
     5821 tcgaatgata agtttaaaga aggcgataat gtattagtta ctggttatga tgttggcgtg
     5881 acacatacag gtggattttc agaatatgca caagtgcctg gagagtggat tgttcctatt
     5941 ccccaagaaa tgtctattaa agagtctatg gtatatggca cagccggttt tacggcagca
     6001 ttatcaattt ttgaactaga aaaatatgga atgaatatca aatcacaacc tgaaatttta
     6061 gtgtctgggg ctacaggtgg tgtgggcagt gttgctatac aaattttaca taaaattgga
     6121 tatgaaaata tcactgctat cgtaagaaaa gaaaagcaaa tagaaatcgc aaaaaaatta
     6181 ggtgcaaaac aagtaataat aattgaagat gataataaac cgcttaaaaa aggtattttt
     6241 gattatttct tagatacagt ggggagtaat gtaacattat acggtttaaa acgtctaaat
     6301 tatcaaggtg ttgctactgt atgtggaaat gtagcaggaa attcacttaa cgctaatata
     6361 ttaccgttta ttcttagagg tattaaatta attggcattg attctgtata tgttgatcat
     6421 aatatccgtg aagatatatg gagtaagtta gcaaatgaat ggaatatagg aaattcactt
     6481 ctatacaatg aaattggatt tgaagaattc tataaaacga tagatcagct tttaaaagga
     6541 caacatttag gaagaactat tttaaaagta taagtgtgag tattcattaa tacatttact
     6601 attaattcaa tcattttgca tttctctact aaccagtgtt ataatttata taacgaaaat
     6661 gattaacaat taatgcacca atccccacac tattgcagta gtgtggggat tttttcgatg
     6721 ctggcttata aagtcgccta ttaattattt gttgcgttta ctaagccaat aagtataata
     6781 cgcaacagca cagccactga tgactggtgc tatgatgtga acgaaaatga ttagcaaagt
     6841 aatgcacctc ctctcttcgt caattgacgc ctgagagata ggcgactcta ttattatacc
     6901 atctgtgaat atacaagcat atctacttcc gataagtcgt cttatgttat ataactttgt
     6961 tttttatagt atcaagacaa gaagaaactc gtttcaaaaa ccctcatgat ttttagagaa
     7021 aaactctctt atttcatcgt ctttaataaa ataatatgcc atctttccat ctttataaaa
     7081 atcaagtacc tcatttttat ataaaagcct taaatgatgt gaagttgaag caacactcat
     7141 tttcaatatc aaagaaatat cacaaacaca caactcatct tctttaatca aagataatat
     7201 gattttcaat ttcttctcat cacaaatttt ttctaaaatg ttaagtaatt tcttagattt
     7261 atcatcttct aaaaaactta aagcattgtt aactttatcc tcatgtacac agatcacatc
     7321 acaagtattt tcataactca tataaaatca caccctaaat taaaatataa atcctaaaat
     7381 tgtttgaata gtgtcatttt caataataat aaataaacct aaagctatat aaataatagc
     7441 cataatccaa cgactaaatt tctcaacaat ttctccaatt cctggaatat tagctaattt
     7501 ttgtgcagta aacaccaaga agaaaattaa aattaaaaag acaaacagag taagtaataa
     7561 attagtaaca cttaatgtca caaaatacgg aacaaataaa ccaatattat cggcgccaca
     7621 acttgctatc gtaacaattg caaccgtacc aactaattta gacaatcctt tttcattcaa
     7681 ttctttttta gctctctttt ctccttcaca atcatcataa atagccactt taattccaag
     7741 ataaattggt attaaaccca ataaacctaa tatccacttt tctggaacat aatttaatac
     7801 aaaagctaaa aacaaactaa ctaatattaa aataatagac cctaaatatt gaccaacata
     7861 aatatctcta tattcttttc tagtctttgc tctagcaaaa aatattaata aaattactaa
     7921 taaatcaact gctgtagcaa tataaagaac agcagcagca acaaccgttt gaatcataat
     7981 acacctcatt caaaaatatt tttgaatgta ttataactta tatttttaaa taatcatagg
     8041 tg
//
LOCUS       DAMQYT010000113         8033 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMQYT010000113 DAMQYT010000000
VERSION     DAMQYT010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.113"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            127..942
                     /locus_tag="PGA33_001838"
     CDS             127..942
                     /locus_tag="PGA33_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002491360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HDG6473642.1"
                     /translation="MHYIKFIESKDNTKLYMKVNDIQDAKANIIIAHGVAEHLDRYDE
                     ITAYLNEAGFSVIRYDQRGHGRSEGKRAFYSNSNEIVEDLDAIINYVKSNFEGKVYLI
                     GHSMGGYTVTLYGTKHPNTVNGIITSGALTRYNNKLFGNPDRNISPDTYIENNLSEGV
                     CSDLEVMEKYKLDDLNAKQISMGLVFSIMDGVRYLKDNAQQFTDNILILHGKEDGLVS
                     YVDSLQLYQEIGSAHKSLHIYDRLEHEIFNESSYNRTIFNEVIEWLETELTYN"
     gene            1264..2442
                     /locus_tag="PGA33_001839"
     CDS             1264..2442
                     /locus_tag="PGA33_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016913246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDG6473643.1"
                     /translation="MSKRSSVVAHYKNGKFQKEFFIQNNKNGYNYLLKYLNDLDHPQL
                     IFESTGIYSRGMERFCCVNQINYIQMNPLEAKFKTSALRSWKTDQADAHKLACLGPTL
                     KQTGSLPIHELIFFELRERARFHLEIENEQNRLKFQILELLHQTFPGLERLFSSRYSI
                     IALNIAEIFTHPDVVLDIDKDVLITHIFNSTDKGMSMDKATKYALQLRVIAQESYPNV
                     DRHSFLVEKLRLLIQQLKQSIHHLKQLDDAMIQLAQQLDYFENIHSIPGIGKLSTAMI
                     IGEIGDIKRFKSNKQLNAFVGIDIKRYQSGHTHCRDTINKRGNKKARKLLFWVIMNII
                     RGQHHYDNHVVDYYYKLRKQPNEKPHKTAIIACINRLLKTIHYLVMNHKLYDYQMSPH
                     "
     gene            2702..3394
                     /locus_tag="PGA33_001840"
     CDS             2702..3394
                     /locus_tag="PGA33_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDG6473644.1"
                     /translation="MTKTYDVWWQKGQESDDDMARDHQEAWERTIKMLDTSDIEGKTI
                     LDVGCNQGGFLRQLYDTTPFKKGVGIDLARLSLEKAETLKGQRPLTYYLTDKPQETKH
                     VFDTAVSTSVLYLIEDIPQHAKDLKEVLKPGGVYYASFADLTNNPSRQFMDDTINQYG
                     ATPSQNHSLKHIVDSFVDAGFEVAVMKEHVPDVIDLTHYSDFYLSPNDYLQTLYEESF
                     LIKASVKEGTEK"
     gene            3391..4944
                     /locus_tag="PGA33_001841"
     CDS             3391..4944
                     /locus_tag="PGA33_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HDG6473645.1"
                     /translation="MRKCVLMTVAASATLLLAGCGNGQKEDKDVTVSLPTEAKADKLD
                     AQGYDAAMPVYSAVYDALVKYDKDKGIKAGLADKWSVDESGKVYEFHLKKNVKFSDGS
                     ALDAKDVKFSIDRAKAMNKDSTVETLKKLDKVVVKNEHVVQIRLKSPSNQVLNELTQV
                     RPLRIMSPHSVEDGKVNGKFEKAIGTGAFVVDKTGKEKTTMKPNKYFDKGHPVNYHLA
                     FQTIEDGDSRNSAVQSGSVDISGGALGMLSDEQIKQDKKNKNLTIEDRPSTVSHFMAF
                     NPKNDVLNQRTIREAISKSIDAKDIAGKSVNGLFQKNVQFVTKNNQQPHDYDMKAAER
                     LLKSEGYHKNDDGIFEKNGKPLSFNLVIQTAEFPNWKDKAEKVQRQLKQAGIKLNVKT
                     LDSQSYYDTLWTKKDYDLIFYRTYSDALMPYNFISSVFKNNDGQPGVLADDETLTKQL
                     DDFPSTVSKEDQQCSFDDIFKHFNQQYYGVPIAYPNETFVVSDKVKQFKFSGLTDAPI
                     DYKALKVNE"
     gene            4947..5903
                     /locus_tag="PGA33_001842"
     CDS             4947..5903
                     /locus_tag="PGA33_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6473646.1"
                     /translation="MLKRTIKFILYLIVSSFIIFILVEKTSGNPAILYLQRHGYTSIT
                     QDNIEAAQHQLGLGQHVLLRYIDWVGHALTGNLGYGFSTNEAVTAMIMEAIVPTLVLI
                     IVSSCIMLPFGYIVGYFVGTRPHTRYANGIRGFAQVMTSMPEYWLAILFIYYLGVRWQ
                     LLPFVGSDSWQHFVLPIFTIVVIEGCHILLMTAHLITQTLDQDAYQLAQLRHFSLKAR
                     IIVQIKEIFAPLMTISINSIIHLIGKAVILEVIFSMSGIGKLLINAINQRDYPLIQGI
                     VIFIIVFIMLMNYLGDVIILKNEPRLRRRHTQQSGNEKRGTM"
     gene            5903..6670
                     /locus_tag="PGA33_001843"
     CDS             5903..6670
                     /locus_tag="PGA33_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6473647.1"
                     /translation="MKKYQTYIAIGSLLSLMVVLITYGLMQDTQHLNPLESPNGQHWL
                     GTDQLGRDFLVRLIVGSLVTLSLTGIVILLSVCMGLIFGLIAGIERRWLDQIIMFVAD
                     MLLAIPSFIIALVILSLVSNSMIGLILALTIGWIGRYLRYFRNLTRDIQKRPFVQYAR
                     LSGNSTFKTTVTHVIPHLLSSIFALVTADFGKMMLSISGLAFLGLGIKPPTPELGTIL
                     FDGKSYFNGAPWLFFFPGVLLGGFALLCQIINKKITQ"
     gene            6637..7404
                     /locus_tag="PGA33_001844"
     CDS             6637..7404
                     /locus_tag="PGA33_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6473648.1"
                     /translation="MSNYQQKNNAVNTVVNVNQLSILDQEKVLLKDVDLTVTKGAFHC
                     IIGESGSGKSLLTRTILGMKQSQLCYQGDIDIDLTQTDAVFQDVQSNMFQNITLAKHF
                     QYIYEANRTHLTKQRIKEDVLQMMQLLGLRQGEQLLERYPFELSGGMAQRVAFIMSLI
                     RRPNYLFLDEPTSALDQENIKKFMHYLLRAQERYQMTIVFITHDINLVKDCATHISIM
                     QQGKLIENGEAASILTKPTHNYTKKLITIAHRRQPYA"
     gene            7397..8032
                     /locus_tag="PGA33_001845"
     CDS             7397..8032
                     /locus_tag="PGA33_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDG6473649.1"
                     /translation="MLKIERLTKYIDTQLIFKEISCTINDQHLLISGESGCGKSTLAK
                     IIAGLDTDYQGELYLNGRLRESYTSKEWMKHIQYVPQYQRDTLNQRKTVLATLLEPLK
                     NYKVNKQRYTSSIEAVLDQCNLPHDILNHKVSTLSGGQFQRVWIAKALILEPEILILD
                     EATTNLDVINEEAILQMLISLKMTQLIIISHDTYVLSQFEGIQLQLNKLNN"
BASE COUNT         2823 a         1241 c         1536 g         2433 t
ORIGIN      
        1 taaaaaacat gggcttaaag ggttaattta tatagtatta ctgcaaacat tgatttgggt
       61 caaaaattag gggtattaaa aaaatgaatg aatcataatt ttatcaagct gattggagag
      121 gttaaaatgc attatataaa atttattgag tcaaaagata atacaaaact ttatatgaaa
      181 gtgaatgata ttcaagatgc aaaagcgaat atcattatag ctcatggtgt ggcagaacat
      241 ttagatcgtt atgatgagat aacagcatat ttaaatgaag cgggttttag tgttattaga
      301 tatgatcaaa gagggcatgg tcgttctgaa ggcaagcgtg ccttttatag caatagtaat
      361 gaaattgtcg aagatttaga tgcgataata aattatgtga agtcaaactt tgaaggtaaa
      421 gtttacttaa tcggtcatag tatgggtggt tatacagtca ctttatatgg aacgaaacat
      481 ccaaatacag tgaatggtat tataacttct ggagcattaa cacgttataa taataaacta
      541 tttggcaatc ctgatagaaa catatcacct gatacttata tagaaaacaa tttaagtgag
      601 ggggtatgtt ctgatttaga ggtaatggaa aaatataaac ttgatgattt gaatgcgaaa
      661 caaatctcta tggggctcgt cttttcaata atggatggtg ttaggtattt gaaagacaat
      721 gctcaacaat ttacagataa tattttgata ttgcatggca aggaagatgg gctagtaagc
      781 tatgtagatt ctttacagct ttatcaagaa ataggatcag cacataaatc attacacatc
      841 tatgatcgtt tggagcatga aatatttaat gaaagttctt ataatagaac tatttttaac
      901 gaagttattg aatggcttga aacggaatta acttataact aaaacagtat agttccgtgt
      961 atttgattat aagaaattat gaggatatta aacatactaa gattagctat gaagaaatct
     1021 atgacgatag attttttcat agctattttt tatagttata gagaggagta gactgtccag
     1081 actcttggat tttaaatccg taaaaaaaac aagtcagctt tactctcacc ttttgaaatt
     1141 cgtttgtagt atgttgggtt cttgaaaccg tgtataggaa aatgaaatga gaaaggttaa
     1201 gtaaagtttt tagcttctca actattcaaa ggaggttttt ttatcgatta cttaggtgtt
     1261 gatattagta aaaggagtag tgtagttgca cattataaaa atggaaaatt ccaaaaagag
     1321 tttttcatcc aaaataataa aaatggctac aattatttac tcaagtattt gaatgactta
     1381 gaccacccac aactcatttt tgaatctaca ggtatctatt caagaggtat ggaacgattt
     1441 tgttgtgtaa atcaaattaa ctatattcaa atgaatccgt tagaagccaa atttaaaacg
     1501 agcgctctaa gatcatggaa aactgatcag gcagatgctc ataagcttgc ttgtttagga
     1561 ccgacgctca aacaaacagg cagcttacct atacatgagt taatattctt tgaattaaga
     1621 gaacgtgccc gttttcatct agaaatcgag aatgaacaaa atcgacttaa atttcagatt
     1681 cttgaattac tccatcaaac attccctggt ttagaaagat tatttagtag tcgatattca
     1741 atcattgcac tcaacatcgc agaaattttt actcatccag acgtggttct tgatatcgac
     1801 aaggatgtac ttattacaca tatattcaat tctacagata agggaatgtc aatggataaa
     1861 gctacaaaat atgcacttca attaagagtg attgctcaag aaagctatcc taatgtcgat
     1921 agacattcct ttctagtcga aaaattacgc ttacttattc aacaattaaa acaatctatt
     1981 catcatctca aacaattaga tgatgccatg attcaattag cacaacaact cgattatttt
     2041 gaaaatattc attcgatacc tggtattggt aagctaagca cagctatgat tattggggag
     2101 attggtgata ttaagcgatt taaatcaaat aaacaactca acgcttttgt aggcattgat
     2161 atcaaacgat atcaatcagg tcatacacac tgtagagata ccatcaacaa gcgtggtaat
     2221 aaaaaagcga gaaaactttt attttgggtg attatgaata taataagagg gcagcatcat
     2281 tatgacaatc atgtcgtcga ttattactac aaactaagaa agcagcctaa tgagaaacct
     2341 cataagactg ccatcattgc ttgtataaat cgattattaa aaacgattca ttatctggta
     2401 atgaatcata aattgtacga ttatcaaatg tcaccacatt agccaaacgt acaattaaat
     2461 atattttaat accttattca aaaaattaaa atgaacggtt tagttaagta atgcttattt
     2521 taattataag tacttgacta atcgtaagaa agagcctagg acataaatca atgtctcgcg
     2581 accacagctt aatttttggt gttcattcat gactttaaaa aatccttatt gcataaatgt
     2641 acatagtgta gtactattca aaacgtaatt attacgattt gaattaagcg aggagaatga
     2701 aatgactaag acttatgacg tttggtggca aaaaggtcaa gaatcagatg atgatatggc
     2761 acgagaccat caagaagctt gggagagaac aataaaaatg cttgatacat ctgacatcga
     2821 agggaaaacg attttagatg tgggatgtaa tcaaggcgga tttttacgac agttatacga
     2881 tacaacaccg tttaaaaaag gtgttggcat agatttagca cgtttatctt tggaaaaggc
     2941 agagacatta aaaggacaac gtccacttac atactattta acagataaac cgcaagaaac
     3001 gaagcacgtg tttgatacgg cagtaagtac gtctgtcttg tacttaatag aagatattcc
     3061 gcaacatgca aaagatttaa aagaggtatt gaaaccaggc ggtgtttatt acgcttcatt
     3121 cgcggattta actaataacc caagtcgtca gtttatggat gacacgatta atcaatatgg
     3181 tgcaacacct tctcagaatc actctctaaa acatatcgtt gatagctttg tggatgcagg
     3241 atttgaagtt gcagtaatga aagagcatgt acctgacgtg attgatttaa cacattatag
     3301 cgatttttat ttatcaccga atgattattt acaaacacta tatgaagaat cgtttttaat
     3361 aaaagcaagt gtgaaagaag gtactgagaa atgaggaaat gtgtattaat gacggtagca
     3421 gcaagtgcta cgctcttatt ggcaggttgt ggcaatggtc aaaaagaaga taaagatgtt
     3481 acggtatcgc tacctactga agcaaaggcg gataaacttg acgcgcaagg ctatgatgca
     3541 gcgatgcccg tttatagtgc agtgtatgat gcattagtta aatatgataa agataagggt
     3601 attaaagcag gtttagcaga taaatggagc gttgatgaat cagggaaagt ttatgaattc
     3661 catttgaaaa agaatgttaa attctcagat ggttcagcat tagatgctaa ggacgtgaaa
     3721 ttctcgattg atcgtgcgaa agcgatgaac aaagattcga ctgtagaaac gttaaaaaaa
     3781 ttagataagg tcgttgttaa aaatgagcac gtggtccaaa ttagattgaa atctccttca
     3841 aatcaagtgt taaatgaatt aacacaagtg agaccgttgc gtattatgag tccacattca
     3901 gtagaagatg gtaaagtaaa cggtaaattt gaaaaagcga ttggaacagg tgcatttgtt
     3961 gttgataaaa ctggtaaaga aaaaacgaca atgaagccaa ataaatattt tgacaagggt
     4021 cacccagtca attatcatct tgcattccaa acgattgaag atggggactc aagaaattct
     4081 gcagtacaaa gtggttctgt agatatttct ggtggtgctt taggtatgct ctcagacgaa
     4141 caaatcaaac aagataagaa aaataagaac ttaacgattg aagatagacc tagcacagta
     4201 agtcacttta tggcatttaa ccctaaaaat gatgtattaa atcaacgcac aattcgtgaa
     4261 gcgataagta agagcatcga tgcgaaagac attgcgggca aatctgtaaa tggtctgttc
     4321 cagaagaacg tacaatttgt gactaaaaat aatcaacagc cacacgatta tgatatgaaa
     4381 gcggctgaaa ggttacttaa atcagaagga tatcataaaa acgatgacgg catctttgaa
     4441 aagaatggca aacctttatc atttaactta gtcattcaaa ctgcagagtt cccaaattgg
     4501 aaagataaag ctgaaaaagt gcaacgtcag cttaaacaag ccggtattaa gttaaatgtg
     4561 aaaacgttag attcacaatc atactatgat acattatgga cgaaaaaaga ctatgatttg
     4621 attttctata gaacgtattc agatgcatta atgccttaca actttataag ttcagtgttt
     4681 aaaaataatg atggtcaacc aggggtgtta gctgatgatg aaacattaac gaaacagcta
     4741 gacgatttcc catcaaccgt atcaaaagaa gaccaacagt gttcatttga tgacatattt
     4801 aaacacttta atcaacaata ctatggtgtg ccaattgctt atccaaatga gacgtttgta
     4861 gtgagtgata aagtaaaaca attcaaattc tctggactta cggatgcacc aattgattat
     4921 aaagcgttga aagttaatga atagcaatgc tcaaacgtac aattaaattc atactttatt
     4981 taatcgtaag ttcgtttatt atcttcattt tagttgagaa gacatctggt aatccagcga
     5041 ttctgtatct acaacgtcat ggttatacgt cgattacgca agacaatatt gaagcggcac
     5101 aacatcaact tggcttagga caacatgtgt tactaagata tatcgattgg gttggacatg
     5161 cactcacggg caacttagga tacggcttta gtacgaacga agcagttacc gctatgataa
     5221 tggaagccat cgtgccgacg cttgtgctaa tcattgtctc tagttgtatc atgttgccat
     5281 ttggctatat tgttggttac ttcgttggga cgcgtccgca tacacgttac gctaatggaa
     5341 ttcgtggatt cgcccaagtg atgacctcaa tgccagaata ctggttagct attttattca
     5401 tttattattt aggcgtacgt tggcaattgt taccatttgt aggtagtgat tcatggcaac
     5461 actttgtgct gccaatcttc acaattgttg ttatagaagg gtgtcatatc ttattgatga
     5521 cagcacatct gattacacaa acgttagatc aagatgcgta tcaactggcg cagttaagac
     5581 atttttcgtt aaaagcgcgt atcatcgtac aaattaaaga gatatttgca ccactaatga
     5641 cgatttcaat taacagtatc attcatttaa ttggaaaagc cgtaatacta gaagtcatct
     5701 tcagcatgtc tggtataggt aaattgttga ttaatgctat taaccaacga gattatccac
     5761 tgattcaggg cattgtcatc tttatcattg tctttattat gctaatgaat tatttaggcg
     5821 atgtgattat tttgaagaat gaacctagac ttcgacgacg tcatacccag cagtcaggca
     5881 atgagaaaag aggtacgatg tgatgaaaaa atatcaaacg tacatcgcaa taggttcact
     5941 attgagtttg atggttgtat taattacgta tggtttaatg caagacacgc aacatttgaa
     6001 cccacttgag tcacctaatg gacaacattg gttgggtacc gatcaattag gcagagactt
     6061 cttagtaaga ctgattgtcg gtagtcttgt cacattgagt ttaacaggca tagtgattct
     6121 attaagcgtt tgtatgggac ttatctttgg cttaattgca ggcatagaaa gacgatggtt
     6181 agatcaaatc atcatgtttg ttgccgatat gttgctggct attccgtcat ttattatcgc
     6241 attagtcatc ttaagtttag taagtaactc catgataggt ttgatacttg ctttaacgat
     6301 tggatggata ggacgttatt tacgttactt cagaaattta acgcgagata ttcaaaaacg
     6361 tccatttgtt caatatgcac gattgagtgg gaactcaaca ttcaaaacga cagtaacaca
     6421 tgtgattcca catttattaa gtagtatatt cgctttggta acggctgact ttggcaaaat
     6481 gatgctcagc atatctggac ttgcttttct aggactaggt attaaaccgc cgacgcctga
     6541 gttaggaaca attctttttg atgggaaaag ttatttcaac ggcgcaccgt ggctcttctt
     6601 cttccctggt gtattgttag gaggtttcgc cttattatgt caaattatca acaaaaaaat
     6661 aacgcagtaa atacggtagt caacgtcaat caattatcga ttttagatca agagaaagta
     6721 ttgttaaagg atgttgattt gacagtaact aaaggtgcat ttcattgcat tataggtgaa
     6781 agtggcagtg ggaaatcact gttaacaaga acaatacttg gaatgaaaca atcacaatta
     6841 tgttatcaag gagatattga catcgattta actcaaacag atgcagtgtt tcaagatgtt
     6901 caaagtaata tgtttcaaaa tataacatta gctaagcatt tccaatacat ttatgaagcc
     6961 aatcgcacac atctcactaa acagcgtatt aaggaagatg tcttacagat gatgcaatta
     7021 cttggtttaa gacaagggga acaattgctt gagcgttatc ccttcgaact tagtggaggt
     7081 atggcacaac gtgtcgcctt tataatgtca ttaattagac gtccgaacta cttattttta
     7141 gatgaaccaa cgagtgcact tgatcaagaa aatattaaaa agtttatgca ttaccttctt
     7201 agggcacagg agcgctacca aatgaccatt gtttttatca cacatgatat taacttagtg
     7261 aaagattgtg ccacacatat tagtattatg cagcaaggta aattgataga aaatggtgag
     7321 gccgcgtcga tcttaactaa gccgacacat aattacacga aaaaattaat tacgattgca
     7381 catcggagac aaccttatgc ttaaaataga gagattaacc aaatatatag acacgcaact
     7441 gatatttaaa gagatatcat gtacaattaa cgaccagcac ttactcataa gtggggagag
     7501 tggttgtggt aaatccacat tagccaagat tatcgctggc ttagatacgg attatcaggg
     7561 cgaattatat cttaatgggc gcttacgtga atcttatacg tctaaagagt ggatgaagca
     7621 catccaatat gtacctcaat atcaacgtga tactttaaat cagcgtaaaa cggtattagc
     7681 tacattatta gaaccactta agaattataa ggtaaataaa cagcgttata catcaagcat
     7741 tgaagcagtg cttgatcagt gtaatttacc acacgatata cttaatcata aagtttcgac
     7801 attaagtggt ggccaatttc aacgcgtctg gatagctaaa gctttaatat tagaaccaga
     7861 gattctcata ttggatgaag ctacaaccaa cttagatgtc attaatgaag aagctatact
     7921 tcaaatgttg atttccttaa agatgacaca attaatcatt atttcacatg atacatacgt
     7981 cttaagccaa tttgaaggaa ttcagttaca gctaaataaa ttgaataatt aag
//
LOCUS       DAMQYT010000114         8027 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMQYT010000114 DAMQYT010000000
VERSION     DAMQYT010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.114"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..380
                     /locus_tag="PGA33_001846"
     CDS             <1..380
                     /locus_tag="PGA33_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDG6473650.1"
                     /translation="IENKESNLVKMDILLNGDKVDALSFIVHRDFAYERGKALVEKLK
                     TLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGGDISRKRKLLEKQKAGKAK
                     MKAVGNVEIPQDAFLAVLKMDDE"
     gene            943..2067
                     /locus_tag="PGA33_001847"
     CDS             943..2067
                     /locus_tag="PGA33_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDG6473651.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRILKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVKRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNAGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDVIALTNRGKVIGNEVFEAFLIND"
     gene            2167..3144
                     /gene="hrcA"
                     /locus_tag="PGA33_001848"
     CDS             2167..3144
                     /gene="hrcA"
                     /locus_tag="PGA33_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDG6473652.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            3176..3802
                     /gene="grpE"
                     /locus_tag="PGA33_001849"
     CDS             3176..3802
                     /gene="grpE"
                     /locus_tag="PGA33_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDG6473653.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            3871..5703
                     /gene="dnaK"
                     /locus_tag="PGA33_001850"
     CDS             3871..5703
                     /gene="dnaK"
                     /locus_tag="PGA33_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDG6473654.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            5839..6978
                     /gene="dnaJ"
                     /locus_tag="PGA33_001851"
     CDS             5839..6978
                     /gene="dnaJ"
                     /locus_tag="PGA33_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDG6473655.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            6982..7920
                     /gene="prmA"
                     /locus_tag="PGA33_001852"
     CDS             6982..7920
                     /gene="prmA"
                     /locus_tag="PGA33_001852"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDG6473656.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            7922..>8027
                     /locus_tag="PGA33_001853"
     CDS             7922..>8027
                     /locus_tag="PGA33_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDG6473657.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDV"
BASE COUNT         3082 a         1111 c         1551 g         2283 t
ORIGIN      
        1 tcatcgaaaa taaagaaagt aatttagtca agatggatat tttattaaat ggtgataaag
       61 tggatgcgct aagcttcata gttcatagag attttgcata tgaacgtggt aaagcattag
      121 ttgaaaaact taaaacgtta attccaagac agcaatttga agtacctgta caggctgcaa
      181 taggacaaaa aattgtagcg cgtacaaata ttaaatcaat gggtaaaaac gttttagcta
      241 aatgttatgg cggtgacata agccgtaaac gtaaattact tgaaaaacaa aaagcaggta
      301 aagctaagat gaaagcagtt ggtaatgttg aaattccaca agatgctttc ttggctgtat
      361 tgaaaatgga tgatgaataa ttttaaaaaa tcaattaaca atttacaatg aataaagttt
      421 aataactaaa aagagggagc ctaggataaa ttaacgtcct gggctttaca atgttatatt
      481 ggcagccatc gacagagtta aaatgagctt ataacaatgg ggccccaaca cagaagctga
      541 cgaaaagtca gcttactata atgtgcaagt tggggtgggg ccccaacata gagaatttcg
      601 aaaagaaatt ctacaggcaa tgcaagttgg ggtgggacga cgaaataaat tttgcgaaaa
      661 tatcatttct gtcccactcc cttatgcatg agttttactc atgtaatttt atttttaagg
      721 acatattaca tctggctaat gtgtaagagc cactacataa taaatcatta gtggttcttt
      781 attatttcta tctcactccc tctaaacaag aataaatatt aaaatgaatc gatatattag
      841 acaatcattg attaaacgtt aaagttaaaa gtaagaataa ttgcagatag tccaacagga
      901 tatagccgat tggataaaaa gtctgagaag cggggcatta aaatgacggt acaaagtgca
      961 tatatacata ttccattttg tgtaagaata tgtacatatt gtgatttcaa taaatatttt
     1021 atacagaatc aacctgtaga tgagtactta gatgcactaa tcacagaaat gtctacagca
     1081 aaatatagga tcttaaagac catgtatgta ggtggcggca caccaacggc cctttctatt
     1141 aatcagttgg aaagattact taaagcaata cgtgatacgt ttacaatcac aggcgagtat
     1201 acatttgaag caaatcctga tgagttaact aaagagaaag tccaactatt agagaaatat
     1261 ggagtaaaaa ggatttcaat gggcgttcaa acattcaagc cggagttatt gtctgtttta
     1321 ggtagaacgc acaatactga agatatttac acttcggtgt taaatgctaa aaacgcaggt
     1381 attaaatcaa tcagtttaga tttaatgtat catttaccga aacagacgat tgaagatttt
     1441 gaacaaagtt tagatctagc tttagatatg gatattcaac atatttcgag ttacggctta
     1501 atacttgaac ctaaaaccca attttataat atgtatagaa aaggcttgct caaacttcct
     1561 aatgaggatt taggtgctga catgtatcag ttgctgatgt ctaagataga acaatctcct
     1621 ttccatcaat acgaaatatc taattttgca ttagatggcc atgaatcaga acataataag
     1681 gtttactggt ttaatgagga atattatgga tttggagcag gtgcaagtgg ttatgtagat
     1741 ggtgtgcgtt atacgaatat caatccagtg aatcattata tcaaagctat aaataaagaa
     1801 agtaaagcaa ttttagtatc aaataaacct tctttgactg agagaatgga agaagaaatg
     1861 tttcttgggt tgcgtttaaa tgaaggtgtg agtagtagta ggttcaaaaa gaagtttgac
     1921 caatctattg aaagtgtctt tggtcaaaca ataaataatt taaaagagaa ggaattaatt
     1981 gtagaaaaga acgatgtgat tgcacttaca aatagaggga aagtcatagg taatgaggtt
     2041 tttgaagctt tcctaataaa tgattaaaaa aattgaaatt tcgagtcttt aacattgact
     2101 tactttgacc aatttgataa attataatta gcacttgaga taagtgagtg ctaatgaggt
     2161 gaaaacatga ttacagatag gcaattgagt atattaaacg caattgttga ggattatgtt
     2221 gattttggac aacccgttgg ttctaaaaca ctaattgagc gacataactt gaatgttagt
     2281 cctgctacaa ttagaaatga gatgaaacag cttgaagatt taaactatat cgagaagaca
     2341 catagttctt cagggcgttc gccatcacaa ttaggtttta ggtattatgt caatcgttta
     2401 cttgaacaaa catctcatca aaaaacaaat aaattaagac gattaaatca attgttagtt
     2461 gagaatcaat atgatgtatc atcagcattg acatattttg cagatgaatt atcaaatata
     2521 tctcaatata caactttagt tgttcatcct aatcataaac aagatattat caataatgta
     2581 cacttgattc gtgctaatcc taatttagtt ataatggtta tcgtattttc atcaggtcat
     2641 gttgagcatg tacatcttgc ttcagacata ccgtttaata atgacaaact aaatacaata
     2701 tcaaattttg ttacaaacaa actaactgag tttaatcaga atttacaaga tgatattgtc
     2761 tcttttgttc aatcagagca ggaagaaatt tttatcaata aattgttaaa tacgatgaat
     2821 aatcatattt ctaatcaaag taatagcatt tatatgggtg gaaaggttaa acttattgat
     2881 gcattgaatg aaagtaatgt atcttcaatt cagcccattc tacaatatat agaatcaaat
     2941 agaattgcag aattattaca agatatatct tcaccaaata ttaatgtaaa aattggtaat
     3001 gaaattgatg acagcttaag tgatatttca attgtaacaa gtcaatatca ttttgatgaa
     3061 acgttaaaag gtcaaatcgc tgtgattgga cctacagcta tgcattatca aaatgtcatt
     3121 caattattaa atagaatttg gtaatgatgg attagaaagt attggaggac agaaaatgac
     3181 aaataaagac gaatcagttg aaaaaaacac tgaatcaaca gttgaagaaa caaacgtcaa
     3241 acaaaatatt gatgattcag ttgaacaagc tgaagaaagt aaaggtcatt tacaagatga
     3301 agcaatagaa gaaacgtctg acgagaatgt tattgaagaa atagatccaa aagatcaaaa
     3361 aattaatgaa cttcaacaat tagcagatga aaacgaagag aaatatttaa ggctctacgc
     3421 tgagtttgaa aattataagc gtagaattca aaaagaaaat gaaataaaca aaacatatca
     3481 agcacaacgt gtgttaacag atattttacc agcaatagac aatatagaac gtgcacttca
     3541 aattgaaggt gatgatgaga cttttaaatc tcttcaaaaa ggtgtacaaa tggtgcatga
     3601 aagtttgatt aacgcactaa aagataatgg tcttgaagtt attaaaactg aaggtgaagc
     3661 atttgatcca aatattcacc aagctgtagt tcaagatgat aaccctgatt ttgaatctgg
     3721 cgaaatcact caagaactac aaaaaggata caagcttaaa gatagagtat taagaccatc
     3781 aatggtcaaa gtaaaccaat aacttaaatt tggcgaaaag acattgttta aaattaaatt
     3841 aatttaatga ttaaatggag gaattttatt atgagtaaaa ttattggtat agacttaggt
     3901 acaacaaatt catgtgtaac agtattagaa ggcgatgagc caaaagtaat tcaaaaccct
     3961 gaaggttcac gtacaacacc atctgttgta gctttcaaaa atggagaaac tcaagttggt
     4021 gaagtagcaa aacgtcaagc tattacaaac ccaaacactg ttcaatctat taaacgtcat
     4081 atgggtactg attataaagt agatattgaa ggtaaatcat acacaccaca agaaatctca
     4141 gctatgattt tacaaaactt aaaaaataca gctgaaagct atttaggtga gaaagttgac
     4201 aaagctgtaa ttacagtacc tgcatacttt aacgatgctg aacgtcaagc aactaaagat
     4261 gctggtaaaa ttgctggttt agaagttgag cgtatcatta atgaaccaac agctgcagca
     4321 ttagcatatg gtttagacaa aactgataaa gatgaaaaag ttcttgtttt tgacttaggt
     4381 ggcggtacat ttgacgtatc tatcctagaa ttaggtgacg gtgtattcga agtactatca
     4441 acagccggtg acaacaaact tggcggtgat gattttgacc aagtaattat tgactaccta
     4501 gttgcagaat tcaaaaaaga aaatggcgta gacttatctc aagataaaat ggcattacaa
     4561 cgtttgaaag atgctgctga aaaagctaaa aaagacttat caggtgtatc acaaactcaa
     4621 atctcattac catttatctc agctggtgaa aacggtccat tacacttaga agtaaactta
     4681 actcgttcta aatttgaaga attatcagat tcattaatta gaagaacaat ggaacctaca
     4741 cgccaagcaa tgaaagacgc tggcttaaca aactcagata tcgatgaagt tatcttagtt
     4801 ggtggatcaa ctcgtattcc agcagtacaa gaagctgtca aaaaagaaat cggtaaagag
     4861 cctaacaaag gagtaaaccc ggacgaagta gtggcaatgg gagctgcaat ccaaggtggc
     4921 gttatcacag gtgacgttaa agacgtagta ttattagacg taacaccact atctttaggt
     4981 attgaaattt taggtggacg tatgaatacg ttaattgaac gtaacactac gattcctaca
     5041 tctaaatctc aaatctattc aacagcagta gataatcaac catcagtaga tgtacacgta
     5101 ttacaaggtg aacgtccaat ggctgcggat aataaaacac ttggtagatt ccaattgact
     5161 gatattccac cagctgaacg tggtaaacct caaattgaag taacgtttga tatcgataaa
     5221 aacggtattg taaatgtaac tgcaaaagac ttaggtacaa ataaagaaca aagaattaca
     5281 attcaatcaa gttcttcatt atcagacgaa gaaatcgacc gtatggtaaa agatgctgaa
     5341 gttaacgctg aagcagataa aaaacgtcgt gaagaagtag acttaagaaa cgaagctgac
     5401 agtctagtat tccaagttga aaaaacttta actgatttag gcgaaaatat cggtgaagaa
     5461 gataaaaaat ctgctgaaga gaaaaaagac gctcttaaaa ctgctttaga aggtcaagat
     5521 atagaagata ttaaatctaa aaaagaagaa cttgaaaaag tgattcaaga attatcagca
     5581 aaagtatatg agcaagcggc tcaacagcaa caacaagcac aaggtgctaa tgctggtcaa
     5641 aacaatgata gtactgtaga agatgctgaa tttaaagaag taaaagacga cgacaaaaaa
     5701 taattaaaca atacaattag aatgttgtca tgatctgaaa agtcaaagtc aatagaacat
     5761 tggctttggc tttttatttg gatagtatga catattacgt taaactatat gagttaatta
     5821 agaaaaggag agatagctgt ggccaaaaga gattattatg aagttttagg cattagtaag
     5881 gatgcttcaa aagatgaaat caaaaaagcg tatcgaaagc tttcgaaaaa atatcatcca
     5941 gatattaaca aagaagaagg tgcagatgaa aagtttaaag aaatttctga agcctatgaa
     6001 gttttaagtg atgataataa acgcgcaagc tatgatcaat ttggacatga tggtccacaa
     6061 ggttttggtg gacaaggatt caatggctct gactttggcg gttttagtgg cttcggtggc
     6121 ggcggttttg aagatatctt tagctctttc ttcggtggcg gcagacaaag agatccaaat
     6181 gcaccgcaaa aaggtgatga tcttcagtat acaatgacat taacatttga agaagcggta
     6241 tttggtacaa caaaagaaat ttcaatccgt aaagatgtaa catgcgaaac atgtcatggt
     6301 gatggtgcaa agcctggaac aagtaaaaag acttgtagtt actgtaatgg cgctggtcat
     6361 gtagctgtag aacaaaacac aattttaggt agagttcgta ctgaacaagt ttgtcctaaa
     6421 tgtaatggaa gtggtcaaga atttgaagaa gcttgtccaa catgtcacgg aaaaggtact
     6481 gaaaataaaa cagttaaatt agaagtgaaa gtacctgaag gtgtcgacaa tgaacaacaa
     6541 attagattag ctggtgaagg ctctccaggc gtgaatggag gacctgctgg tgatttatac
     6601 gtagtattta gagttaaacc atctgaaaca tttaaacgtg atggtgatga tatttattac
     6661 aaattaaatg ttagcttccc acaagcagct ttaggcgatg aaataaaaat accaacatta
     6721 aataatgaag ttatgttaac aatccctgct ggtacacaaa caggtaagca attccgctta
     6781 aaagaaaagg gtataaaaaa tgttcatggc tatggatacg gagatttata tgttgatatt
     6841 aaagtagtaa cgcctactaa attaacagat agacaaaaag aactaatgaa agaatttgcc
     6901 caattaaatg gtgaagaaat taatgaacaa ccttcgaatt ttaaagatag agcaaaaaga
     6961 ttctttaagg gagaataaag tatgaactgg acagagcttt caattattat taatcatgaa
     7021 gcagtagaat tggctaccaa tatacttgaa aatcatggat caaatggtgt cgtgatagaa
     7081 gattcagatg atttaattaa ccaaccagaa gataaatacg gtgaaattta cgctttgaaa
     7141 aaagaggatt atccagataa gggagtaaga ttgaaagcct attttaatga aatgacttat
     7201 gatgataagt tgcgacagca aattaaagat gagttattaa atttagatga acttgatcaa
     7261 cataacgttc aattcagtga gcaaattatt gcagagacgg attgggaaaa tgaatggaaa
     7321 aactatttcc atccattccg agcgtcgaag aagttcacaa tagttcctag ttgggaaaca
     7381 tatgctaaag aagcggatga agagctttgc attgagctcg acccaggtat ggcttttgga
     7441 acaggtgatc atccgactac aagtatgtgt ttgaaggcaa tagaaacata tgtattgcca
     7501 cagcattcag taattgatgt tggtactggc tcaggtatat taagtattgc aagtcatcta
     7561 atcggtgtaa aacgtattaa agcgttggat attgatgaaa tggcagtgag tgtagctaaa
     7621 gaaaacttca gaagaaatca ttgtgaaacg ttaattgaag ctgttccagg taacttattg
     7681 aaagacgaaa cagaaaaatt tgatattgta atagcaaata ttttagcgca tattattgat
     7741 gaaatgattg aagatgctta taatactcta aatgaaggcg gttattttat tacttctggt
     7801 attataaaag agaagtatga aggtatacag tcacatatgg agcgtgtagg ttttaaaatt
     7861 atttcagaac aacatgacaa cggttgggtt tgtcttgttg gccagaaagt gagtgaataa
     7921 tgtgcaacgt tatttcatag accaaaacgc tgatgtaagt cagcgttttt ttattacaaa
     7981 aaaagaagat attcatcata taacaaatgt aatgagatat gatgttg
//
LOCUS       DAMQYT010000115         7897 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMQYT010000115 DAMQYT010000000
VERSION     DAMQYT010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.115"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(221..2677)
                     /locus_tag="PGA33_001854"
     CDS             complement(221..2677)
                     /locus_tag="PGA33_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDG6473658.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            complement(2691..3698)
                     /locus_tag="PGA33_001855"
     CDS             complement(2691..3698)
                     /locus_tag="PGA33_001855"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDG6473659.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            complement(3688..4254)
                     /locus_tag="PGA33_001856"
     CDS             complement(3688..4254)
                     /locus_tag="PGA33_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDG6473660.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            complement(4273..4734)
                     /locus_tag="PGA33_001857"
     CDS             complement(4273..4734)
                     /locus_tag="PGA33_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDG6473661.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            4892..6106
                     /locus_tag="PGA33_001858"
     CDS             4892..6106
                     /locus_tag="PGA33_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDG6473662.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            complement(6314..6874)
                     /gene="pdxT"
                     /locus_tag="PGA33_001859"
     CDS             complement(6314..6874)
                     /gene="pdxT"
                     /locus_tag="PGA33_001859"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDG6473663.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(6878..7765)
                     /gene="pdxS"
                     /locus_tag="PGA33_001860"
     CDS             complement(6878..7765)
                     /gene="pdxS"
                     /locus_tag="PGA33_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDG6473664.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
BASE COUNT         2319 a         1531 c         1105 g         2942 t
ORIGIN      
        1 acgtaagttg ctagtatctt caaacaaaaa acctttataa aaattcatac ctttatgcta
       61 tcgctgtagg ctcattaact tgttacatat aattcttaac tatcctttga tgattgtttt
      121 attagattgt ttcgttgatg gatactttca cgaatttcta tagttcaatg ctactaaaaa
      181 aacagcccaa aactttaatt tgttttggac tgttttataa ttatgcttgc gatggtgttt
      241 tagtttctga agtttgttca gcaatgtcat atttaaactc tttaccatca tgatctactg
      301 taactttctt accttcaatt tgattaccat ctaatattaa ttcacttaaa ttatcttcga
      361 tagttttttg tatcgctcta attaatggtc ttgcaccata ttctggatca tatccttctt
      421 ctgcgatttt gtctttcgct ttatcagtta caataatatt tatgttttgt tcagataatc
      481 gatttgttaa tttattaacc atcattgtta caatttcttt taattcttct tttgttagtt
      541 tatggaatac aatgatatca tctacacggt ttaaaaattc tggacggaat gaatttttta
      601 attcttttaa catcgttttt cgaattgttt cataatcttg tccatcactt gaaccaccga
      661 atccagcaaa tcgttgatct tgtaattctt gtgccccaac gtttgatgtc attatgataa
      721 ttgtatttct gaaatcaact gtacgtcctt ttgtatctgt caaatgtcca tcatctaaaa
      781 cttgtaatag aatattaaat acatctggat gagctttttc aatttcatca aataaaatta
      841 cagaatatgg tttacgtcta actttttcag ttaattgtcc accatcatca tgaccaacat
      901 atcctggagg agcaccaact aatcggctca ctgcgtgttt ttccataaat tcactcatgt
      961 ctacacggat catcgcatca tcatcgccaa acattgattc agctaaagct ctagctaatt
     1021 cagttttacc aacaccagtt ggtccaagga agataaagct accaattggt cgtttaggat
     1081 cttttaaccc tgcacgggca cgtctaaccg ctttactgat tgaattaaca gcatcttttt
     1141 gcccaataac tctctcatgt aatgtatctt ctagactaag aagtttttca gattctgttt
     1201 cattgatttt agttaatggg atacctgtcc atcctgcaat aacttcagca atatcttctt
     1261 ctgacaatga agttgacatg ccattttgtg cattcttcca ttcattttta gcttcttcat
     1321 attgcttttc aagttttgtt tgtttatcac gcaggttagc agcattttca aactcttgag
     1381 catgtactgc ggcatctttt tcatttttaa ctttttcaat ttcttgttca atttctttta
     1441 aattattagg tgtcgtatga ctcttaagtc ttactttaga acttgcttca tcaattaaat
     1501 caattgcttt atctggtaag aaacgatctg aaacgtatct gttacttaat ttaacagctg
     1561 cttcaatagc ttcgtctgaa atattaatac gatggtgtgc ttcgtaacga tctcttaatc
     1621 cttttaaaat agcaactgta tctactactg aaggttcatc aacttgtaca ggttggaaac
     1681 gacgttctaa agccgcgtct ttttcaatat ttttgcgata ttcatctaat gtagtagcac
     1741 caatacattg taattcacca cgtgctaatg ccggcttcaa aatattcgaa gcatcgatag
     1801 caccttcagc accaccagca ccaactaaag tatgcaactc atcaataaat aggatgacat
     1861 tacctgcttg ttggatttct tccataacct ttttcagacg ctcttcaaat tcaccacgat
     1921 atttagtacc tgcaactact gttcccatat ctaaagacat aacacgctta tcttttaatg
     1981 tctctggtac ctcattattc actatggctt gcgctaaacc ttcagcaata gcagttttac
     2041 caacacctgg ctctccaata agcacaggat tgtttttcgt acgtctactt aatacttcaa
     2101 ttacacgtgt aatttcttta tcacgtccta taacaggatc taatgtaccg tctttggcaa
     2161 tgactgttaa gtcacgagct aaactatcta aagttggagt attatttgac ttactagctt
     2221 gtgcattttt attactcatt tcagggtttc ctaaagcttt cacaacttgt gcacgtgctt
     2281 tagtaatatt taaatctaga tttgcaaaaa ctcttgctgc aacaccttca ttttcacgaa
     2341 tcaagcctaa taaaatatgt tccgttccaa caaaattgtg atgtaatttt ctagcttcat
     2401 ccatcgataa ttcaatgact tttttagctc taggtgtata atgcaatgta ccaacatgat
     2461 cttgaccatg tccgattaat ttttcaactt cttcaattac tttatcttca gtgatattaa
     2521 aactttctaa tacttttgca gcaattcctt caggttcttt cattaacccc aataataggt
     2581 gttctgttcc tatatttgaa tgatttaaac gaattgcttc ttcttgggca tgtgctaata
     2641 cgcgctgtgc acgctcagtt aatctaccaa ataacataaa taatgacctc ctactttata
     2701 tgttctctta gtatatctgc tcgtttttct tttacagatt tgtcatcttc ttcatctaat
     2761 aaaaatggtg actgtatagc taccatcaat tcattaaatt taaagttttg taattcaatg
     2821 taatttaaat ctataccaag tttaacttcg cttaatctat aagaagcctc ttccatagtt
     2881 atcattctac agttttgtaa aatacctagc gagcgaaaaa cacggtcttg tgtttctaat
     2941 tgattataag tgtctaactt ttgtcgtatt tgtttttctt catgaatgat ttgattaaca
     3001 acttctgtta atgtttctat gatttctaac tcagatttac caagtgtaag ttggttggat
     3061 acttgataag tatgtccata aacttgcgaa ccttcaccgt aaatacctct gattgtatat
     3121 ccaaaacgat taatggtttg agcaatccgt gtcattcttt tcataataga tagacctggc
     3181 aaatgtagca tcacgcttgc tctcatacca gtacctatat tggtaggaca tgtagttaaa
     3241 taaccaagtt gttcatcata acttatatca aggcttcgat ctaattcatc atcaattgat
     3301 gaagcttgat tatataaagc ctgtaatgtc gtgtcagttc ccatagcttg aatacgaata
     3361 tggtcctctt catttatcat gacacttaaa gattcatcgt cattcactaa tactgcggct
     3421 gctggttgtt ttattagttc aggactaatc aaatgctttg caaccatttt cattttactt
     3481 tgttgatcca tttgatcaag acgcattaat tcaaagtttg gcaaggcatc ttgtacctca
     3541 tttataactc taaatccatc attttcagta gcatacatta gtggatgcac atgattttct
     3601 aaattacgcg ctaaccgaat tctagaagac ataacaattg gtgtttcttc attacttttc
     3661 atccattggc tgatattatc atgaatatta tgcgtcatca tgttgcacct cactctcagc
     3721 ttttagtgct ttaatttcat ctctaacaat ggctgcttcc tcaaaatctt ggatttcaat
     3781 aagttttttc aaatattcat tcttttcttc gatttttcgc tttaaagcta tctttttatg
     3841 tgaagaatgt ggtgtctttc caacgtgctc aaattgtcca ccttgaactc tgcggacgat
     3901 atcaatgatg tcatctttaa atgttgcata acaattagca cacccaaatt taccaacatg
     3961 tgcaatatct ttcaaagtca tatgacatga tgggcaacgt ttttcttctt taaacgctac
     4021 ttcttgaaaa ttaattccat gtttcgtagc taaatgttgt aaaatttgct taacaacaaa
     4081 tgcttcttcg aaattatctt gatgttcttg atattcaggt tgttcattag cttgattcca
     4141 cggatggtgt ccctcagcac aagtttgaca caccattttt tcttctgttt tatttttact
     4201 tgtaacttta acttttaatt ccgcttcatt aagttgacaa ttttcacaaa gcacttcaac
     4261 acctcatttc atttagtaat aatttataac tggtaacaaa cgttttaaaa tatttgctct
     4321 aataatatct ctagaaacca tatccattga tagcgtttct ctatcaataa ctgcttgaat
     4381 cattttagct tcacgttcat ttattaacat tttatctaaa agcccatcaa taatataata
     4441 agcttgttgt tgagaaatag aaggtccaat cagctgaagc aaatgattaa tataacctgt
     4501 tgcatcttta ttttcaattt tagtgattcg gatgtaacca ccaccaccac gtttactttc
     4561 gatttcataa ccatgttcat tagtgaatcg tgttttgatt acataattta attgtgatgg
     4621 tacgcaatca aaacgctgtg cgatattcgc tctctgaatt tcaacgacat cttcattcga
     4681 ctcttcaaat aaacgtttga tgtattgttc tatgatgtca gacatattgt gcatgtatat
     4741 cacccctttt tgaccttctt tgactatatt atatgaccta ttttgacctt tttcaattaa
     4801 tttgttttta tattttcaaa aatttatgat gttagccctt ccattttatt gtatgataga
     4861 ttgtagaaaa aactaaagga atgagaaata tatgcatatt ctaattggga ttatcggaat
     4921 tatcttcttt ttagcacttg cgtttctatt tagctcagac aggaaaaata ttcgctggaa
     4981 atatgtagga attttacttg caattcagct tatctttgca tttatcttgc ttaaaactac
     5041 aatgggaatc aaggttattg gtggtatttc aaacggtttt aactacttat tattaaaagc
     5101 agcagatggt gttaactttg tattcggtgg tattcaatat atcgatccta agcaaccacc
     5161 attcttcttc agtgtattat tacctatcgt atttatttca gcgattatcg gtatccttca
     5221 atacacaaag attttacctt taatcattaa tgttttagga ttcttaattt ctaaaattaa
     5281 tggtatggga cgtttagaat catacaatgc agttgctgca gcaattttgg gacaatctga
     5341 agtatttatt tcaattaaaa aacaattacc atacatacct aagcaacgtt tatacacatt
     5401 aacagcttca gcaatgtcga ctgtttctgc ttcaattatc ggtgcatact ttaccttaat
     5461 tgaacctaaa tatgttgtaa cggctgttgt cttaaactta tttggtggtt tcatcatcgc
     5521 ttcaattatt aacccttata aagttaatga agaagatgac aaattattag ttgaagaaac
     5581 tgaagaaaga caacaatcat tcttcgaaat gctaggagaa tatattttag atggatttaa
     5641 agtagctgtc attgtaggcg caatgttaat cggatacatt gccattatcg ctctattaaa
     5701 tggaattgta agcaatatct ttagtacagt ttcaggtggc gctatttctt gggatttcca
     5761 aacattaatt ggttttgtct ttgcaccatt tgcattctta gtcggtgtgc catggcaaga
     5821 tgcagttcaa gccggttcag ttatggcgac aaaactatta tctaacgaat ttgtagcaat
     5881 gcaagcactt ggtaaactta gtgatttatc tgaacacgca aaaggtgtta catctgtatt
     5941 cttagtatca tttgctaact ttagctctat cggtattatt tcaggtgcga ttaaatcact
     6001 taatgacaaa aaaggagata cagttgcccg ctttggtttg aaattattat tcggtgcaac
     6061 actagtatct ttcatttcag ctgcaatcgc tggattcttt atttaacaaa tacatcaaca
     6121 tcgaattaac cccttcaaaa ttttgactat tctcagtctc tttgaagggt ttttttctta
     6181 tgtatagctt tattttttat catttttaat tttgacataa aaaataactc ctccaaagct
     6241 aagtttgaag gagatatttt cttctcaaca aatctataaa tatcaataat tcgttgattt
     6301 agcatacatt aagctatgct ttttttacaa tatgattaat aaagtaatca gttactctat
     6361 agtcatctgt taattcagga tggaatgata cgcctaaata tttaccttgc tgaacagcta
     6421 caattttctc attaacctta cataggatat ctacgccttg acctactttt tcaatatgtg
     6481 gggctcttat aaagacacct tcaatatctg tagcgatacc tttaatatct aattctgttt
     6541 caaagctgtc aacttgtcta ccgaatgagt ttcgttgtac agtaatattc aacttgttaa
     6601 ggtatccttc ttcaccaact atatcttgcg ctagaactat taatcctgcg catgtaccaa
     6661 acataggtaa agttgaattt tgtaaagcct ctttaaatcc atataaattc attaatcgac
     6721 gtaacgttgt agactcgcca ccaggtaata ttaagccctc gatttcttct aattgttcaa
     6781 cttttttaac tgcaatacct tcatgaccac ttaattcaat atgtctaata tgttcacgta
     6841 ctgcaccttg taatgctaat acacctattt tcatatctta ccaaccacgc tcttgcatac
     6901 gttcttctaa tgataattga ttgatatcta aacctttcat agcagtgcca agttcacttg
     6961 ctaatcttcc aattagttca tagtcttggt aatgtgttgt tgcttgaaca attgctttag
     7021 caaatttttc tggatcttct gatttaaaaa tacctgatcc aacgaataca ccgtcagcac
     7081 ctaattccat cattaaagca gcatcttgag gagtcgcaac gccaccagct gcaaagttaa
     7141 ctaccggtaa acgaccattg tctttaattt gttttaaaat ttcataaggc gcaccgatat
     7201 ctttcgcaaa agtcataatc tcatcatcat tcattacagt caatcgacta acttctgaat
     7261 taacttgtct catatgtctt acagcttcaa caatattacc tgtacctggt tcacctttag
     7321 tacgtaacat agcagcacct tcaccaattc tacgcgcagc ttcacctaaa ttacgacatc
     7381 cacatacaaa tggtactgta aattgatctt ttcttaagtg atattcctca tctgctggtg
     7441 ttaacacttc tgattcatca atatagtcaa cacccatcgc ctctaatact cttgcttcag
     7501 tgatatgacc aatacgtgct ttagccatga ctggaataga aacagcattc attacttctt
     7561 ctacaatttt agggtttgcc atacgtgcaa caccaccagc agctctaata tcagaaggta
     7621 ctcgttctaa tgccataact gctaccgcgc cagcttcttc tgcaattctt gcttgctcag
     7681 cattaacgac atccataata acgccgcctt tttgcatttc agccatacct cttttgactc
     7741 tgtctgatcc aataatttta ctcattatac ataaaacccc ttttcaagtt gattaaatac
     7801 attgtaatgt ataaactgat atttaaaaag gtacaaaata agataaattt aggaggtcag
     7861 ttataaaatt gaaaaacact ttataccatc aacttta
//
LOCUS       DAMQYT010000116         7865 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMQYT010000116 DAMQYT010000000
VERSION     DAMQYT010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.116"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            176..379
                     /locus_tag="PGA33_001861"
     CDS             176..379
                     /locus_tag="PGA33_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDG6473665.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            complement(376..612)
                     /locus_tag="PGA33_001862"
     CDS             complement(376..612)
                     /locus_tag="PGA33_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473666.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            837..1418
                     /locus_tag="PGA33_001863"
     CDS             837..1418
                     /locus_tag="PGA33_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDG6473667.1"
                     /translation="MAKTLYIMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGIFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEERIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            complement(1491..2108)
                     /locus_tag="PGA33_001864"
     CDS             complement(1491..2108)
                     /locus_tag="PGA33_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HDG6473668.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGETQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF"
     gene            complement(2263..2757)
                     /locus_tag="PGA33_001865"
     CDS             complement(2263..2757)
                     /locus_tag="PGA33_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6473669.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
     gene            complement(3156..3578)
                     /locus_tag="PGA33_001866"
     CDS             complement(3156..3578)
                     /locus_tag="PGA33_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDG6473670.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            3726..4442
                     /locus_tag="PGA33_001867"
     CDS             3726..4442
                     /locus_tag="PGA33_001867"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDG6473671.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFTNDSYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     VPQAPGQIDVTDLKAQVTLY"
     gene            4525..5064
                     /locus_tag="PGA33_001868"
     CDS             4525..5064
                     /locus_tag="PGA33_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDG6473672.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEGDMPKAPRKNRNLITLY"
     gene            complement(5214..5534)
                     /locus_tag="PGA33_001869"
     CDS             complement(5214..5534)
                     /locus_tag="PGA33_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDG6473673.1"
                     /translation="MQSIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            5678..6034
                     /locus_tag="PGA33_001870"
     CDS             5678..6034
                     /locus_tag="PGA33_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDG6473674.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
     gene            6192..6572
                     /gene="gcvH"
                     /locus_tag="PGA33_001871"
     CDS             6192..6572
                     /gene="gcvH"
                     /locus_tag="PGA33_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDG6473675.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVAIIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQIEALLTAEKYSEMIGE"
     gene            6751..7629
                     /locus_tag="PGA33_001872"
     CDS             6751..7629
                     /locus_tag="PGA33_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HDG6473676.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQ
                     EETLNQSKIGGMPFLKYFKDIPLDENNVPMVLLAQINLDDLPEQQELFPVKEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQAHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
     gene            complement(7717..>7865)
                     /locus_tag="PGA33_001873"
     CDS             complement(7717..>7865)
                     /locus_tag="PGA33_001873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473677.1"
                     /translation="FFNSSQGPQHREFRKEILQAMQVGDGPQQREIGFPISTDNASWG
                     GTTK"
BASE COUNT         2827 a         1115 c         1324 g         2599 t
ORIGIN      
        1 aaaagaatat ttacatacta aaaactctta aagttgtata attcgttaat atttttagaa
       61 tcagatatca aaaaaatgtg caaaatcact tttgcacatt tacaaatact agttttctga
      121 taagattaat aataacactt taaatagcgc ttaataaatg aagggggcaa gtcttatgac
      181 gttttacaat ttcatcatgg gttttcaaaa tgataacaca ccatttggta tattggccga
      241 acacgttagt gaagataaag cattccctcg attagaagaa agacaccaag taattagagc
      301 atatgtgatg tctaattaca cagatcatca attaattgaa actacaaata gagctattag
      361 cttatatatg gcaaattaat ttgagtagta ccaattatga tgtattagtg catcccaaat
      421 atcttttgtt ttaaagttta tttcatcatt tcttatcgaa aatggtgtaa taatgtcttt
      481 atctaaccaa gtgttgataa gttcatttgg tacaccatct aacaacattt cacttttact
      541 aattataaaa cattcccagt caagtgaaac attttgtgga ttcacataat tacattgatt
      601 atgattatcc ataaacactc actcctttaa aattctgtac tcttcattgc gttttacccc
      661 gtcacattat cttttaaact aaaatcatca ttacttatga aaaaaatgta catcaaaagc
      721 aaaggttttc gctaccgaaa aagtttaaat aatggtttaa tatatttggt actcatttta
      781 ataaaaagag aatacatttt gagctatcaa tactttttat tgaagaggtg ttattgttgg
      841 ctaaaacgtt atatataatg cgccacggac aaactttgtt taattttaag ggactaattc
      901 agggatttgg agattcgccg ctaacagaac ttggaattgc tcaagctcaa aaggcacgta
      961 gttattatga aactaagggg ataaacttcg atttatatgc atcatcaacg caagaacgcg
     1021 caagtgacac acttgaaaat gttgcaccta accaatcgta tcaacgtttt aagggactga
     1081 aggaatggca ttttggaata tttgaaggtg agtcagtcta tctatttgat aatctataca
     1141 agcctgagga cttattcgga gatcgaattg ttcctttcaa aggagaggca aggcaacaag
     1201 ttgaagaacg cattgtgaaa actttacatg acattatgtc tcaaacaaag aataatgcat
     1261 tagtcgtgag tcatggaaca ataatgggag tatttttaag atattgcctt aaactagatg
     1321 aagcattaaa gcataatatc ggtaattgta atatcctgaa atttgaatat gacaatggaa
     1381 catttaaatt tgttgagtta attgatccaa atttataata aataaagcgt tatacataga
     1441 cacaagtatg tcttttgtat aacgctttta aaatacaact acataacgct ttagaataat
     1501 aattgaataa gtttttgtaa tatcatcaga gcaacaataa taattattat gcttgatatt
     1561 ttattgatta ttgttaataa ttttccggtt ttatcaattg atccaaccat ttttcctaaa
     1621 attgcgagta aaaagaacca taaccatgaa acgctaatac aagcgattgt aaacgcaatt
     1681 ttattgctgc cactatataa tgcagcacta ctaccaatta ctccaattgt atctaaaata
     1741 gcatgtggat tgagtaatga aactgataaa gcaaaactta cttgtttcat tggagacata
     1801 atttgagttt ctccatctgt tgagggttta tcatgccaaa tggtccaagc catatacatc
     1861 aagaaaatta aaccaactat ataaataatt gcttgaagta caggtaaaga cataataatg
     1921 atagatactc ctaccactgc aataataata agtaagctgt ctgacaaccc ggctgtaatt
     1981 attgcaggca atacatatct atattttggt tgattagctc cttggttaaa aataaataca
     2041 ttttgtgcac ctaaaggtaa aattagacca atcgctaaga taaatccatg aataattgcg
     2101 gttaccatta agttttgaca cccctaaaac atagaatatt ctgaaaaaac ttattgttat
     2161 catatcaaat aaaaaacaac tactcaatag tagagtagtt gtatgaaaaa tagaacctat
     2221 atgatttgaa tttatgagaa atgtagaata tataaaactg tattacttat taatcaaatc
     2281 ataaaaaagc caatgttcat cgaaataggt atttccgatt ttaattgcat ttttctcaaa
     2341 accaagaatg tcgaatccta gtgcactata gaaaacttta gcacctatgt tgtttgacac
     2401 aattgatgta agtaaagatt cgtaattgtt ctgccgtgca taattaataa tgaaattaat
     2461 gagctcgcta ttaatcgatt tatcgttatt agtaacaaag ttgtatttaa ttaatgattt
     2521 atgttctttt ccaacgtatc gaatttgttc taaggcagct gttgcgataa gtgtttcgtt
     2581 ttcataacag ccaaatacaa tacatttcgg tgaaccttga tttaaaatgt catgtattaa
     2641 ggtgtcagtc attgtttgac tataatgtga atcttgagtg aattcttcat tgactgtcga
     2701 aacaagtctc ttatactcat taaaatcatt attcgttaat gttttaattt caatcattac
     2761 tgacctcctt aatcattatt aagaaagatt ttgtactaaa aaaatttctt aattacatat
     2821 taaaatgttt attaaaattt tgcaatcact gatttatatg cacattcttg taaatggatt
     2881 atcaatttaa ttatatttat ttttaagttc aggaaattat ggaattggtt attggtcgac
     2941 actggacttt tatttgtaga cggtatgcga agaagtcaag ccagtattgg aaccggtcac
     3001 atttatgatg actaaggtac aatcattttc agttaaatat tgccagaaat ttcaatcacg
     3061 aatagaaaaa ataaccaacc aaaaattttt ggttggcaaa tttgaatttt atgtgaattt
     3121 tattaataac gttgaataat ctctttaagt taatgctaat ctacaacatt tacatttaaa
     3181 tcgacattaa tatttccttg agtcgctttt gaatatggac aaaattcatg agccatttgt
     3241 aaatattttt cagcttcttc ttgagatata acatttttaa ttgtcgcatc aattgaaaca
     3301 cttaatttag gactttctga gtctgaatca tcttctagtc tcactgttag tgttacttct
     3361 ggatgagcat cacgcacttt gttttgcttt aaaattaggt cgaaagcacc gttgaagcaa
     3421 gatgcataac ctgctgcaaa taattgttcg gggttagtag ctttaccatc tgcttgagca
     3481 ggcggaacga tatcaatatc taatgctcga tcatcagtat aaacatgtcc tttacgtccg
     3541 ccaacattcg tcgctttagt ttcataatgt attgccatac ttataacctc ctaatttaaa
     3601 atacatttat gctttaccca ttcgaaaata ccttaatcat tttcatttat agcattgttt
     3661 gattgaagga taaaaagttg ttgtttacaa taaaaataat gagtatctga aatgagggat
     3721 tcactatgac acatgtggaa gtagtagcga ctatcgcgcc acaattatct atcgaagaaa
     3781 ctttaattca aaaaattaat catcgtattg atgcaataga cgtattagaa ttacgaattg
     3841 atcaaattga aaatgtcaca gttgatcaag tggcagaaat gattacaaag ctgaaggtta
     3901 tgcaagattc attcaaatta ttagttacgt atcgtacaaa gttacaaggt ggctatgggc
     3961 aatttacaaa tgactcgtat cttaatttaa tatcagactt agcaaatatc aatggcatag
     4021 atatgattga tatagaatgg caagcagata ttgacattga aaaacatcaa cgaatcatta
     4081 cacatttgca acagtataat aaagaggtgg ttatatcaca tcataatttc gaaagtacgc
     4141 ctccattaga tgaattgcaa tttatatttt ttaaaatgca aaaattcaac ccagaatacg
     4201 ttaaattagc agtaatgcca cataataaaa atgatgtgtt aaatttattg caggcaatgt
     4261 ctacattttc agatactatg gactgcaaag ttgttggtat ttcaatgtct aaacttggac
     4321 taataagtag aacggctcaa ggcgtttttg gtggtgcgtt gacttatggt tgtatcggag
     4381 taccacaagc tccaggacag attgatgtta ctgatttaaa agcacaagtg actttatact
     4441 aataaaatga attaggttgc ttttgtgtaa gtgctgtctt ataattgaga agtattatca
     4501 ttgttcgtgg aggagcgatt ttagatggaa ttacaacaag caatagctaa tagaagaagt
     4561 gtgaaaaaat ttaaaagaga tatgcacata gatgacgcat tgctatatca agcaattgag
     4621 aaagctgctg atgctccaaa tcacggaatg agggaaccat ggagagttgt gcatgttccg
     4681 aaagacagat taggagatat gagtaaggat atttctaaat ttgcatttcc taatgaatta
     4741 gataagcaac aatgtcatta tgatgcagtt acgaaactag gtggcatgtt attgcttatt
     4801 ttaaaaacag atccaagaca acgtcaaaat gatgaaaact actttgcatt tggtgcatat
     4861 gcacaaaatc ttatgttgtt actttatgaa gcgggaatag gtacatgttg gaaatcgcca
     4921 ttatatatct atgatcctaa agtaagaaaa acacttggta taaagaaaga tgaagttctt
     4981 gctggattct tatatttaac ggatttagaa ggagatatgc ctaaagcacc acgtaaaaat
     5041 agaaacttaa ttacattata ttaatatgta taattataga aacattaata aaagctgagt
     5101 catgaattga tggacatcta tcgagttaga gatttaatct aacttactag agtcggtaca
     5161 ataacagtct cagcttttta ttgtgcagta tatacacatt tttattttag tatttattta
     5221 aaagtttctg ctaaaaatga ttcaacttgt tcaggtgact tagcatttgc tgaatgaagg
     5281 tgtgcaattt tatcgccgtt tttaaatact agcaagctag ggatacccat aacttcattt
     5341 tcaacaacta catcttctaa ttcatcacga ttaacagtat accattggta atcattatat
     5401 tgttctacga ttgggtcaat ccataaatcc atagcacgac agtctgggca ccatcctgcc
     5461 tcaaatttaa caattacagg tgtatcgcta ttaattacag atttaaatga ttcattactt
     5521 ttgattgatt gcattgtaac aactcctcta gatagtttaa taatttttat tatagctaaa
     5581 tttatatcat aataaaaaat tttagcttca aaatgaaagc cttttgcttt ggaaaatgat
     5641 atatttattt taaatacata aaggaggttg cagtcgtatg attaaatttt accaatataa
     5701 gaattgtaca acttgtaaaa aggcagcaaa gtttttagat gaatatggcg taagttatga
     5761 accaattgat atcgttcaac atacacctac aataaatgaa tttaaaacaa taattgcaaa
     5821 tacaggcgta gaaattaata aattgtttaa tacacacggc gcgaaatatc gtgagcttga
     5881 tttgaaaaat aaattacaaa ctttatcaga tgatgaaaag ttagagttgt tatcatctga
     5941 tggtatgtta gtaaagcgtc ctctagcagt aatgggcgat aagataacat taggatttaa
     6001 agaagatcaa tataaagaga cttggttagc gtaagtgaaa tgtaagcgtt tactaaatat
     6061 ctcgatattt agattcatta catgtaaaat gaaataagct atacaattgt taatttttat
     6121 aaatatagtt gaatagcatc tagccttatg gcatcattaa tgatgtaaag attaattagg
     6181 aggggattct cttggcagta ccaaatgaat tgaaatattc aaaagagcat gaatgggtta
     6241 aagttgaagg taatgtagca ataattggaa tcacagaata cgcacaaagc gagttaggtg
     6301 atattgtttt cgttgaatta ccagaaacag atgatgaaat taatgaaggg gatacgtttg
     6361 gtagcgtaga atcagttaaa actgtatcag aattatatgc accaatctct ggtaaagtag
     6421 ttgaagtcaa cgaagaacta gaagatagtc ccgaatttgt aaatgaatct ccatacgaaa
     6481 aagcatggat ggtaaaagta gaaattagtg atgaaagtca gattgaagct ttattaacag
     6541 ctgaaaaata ttcagaaatg attggtgaat aatcaccgtg taactcctta atctaagatt
     6601 gaggagttta tttttagtct gagaaaataa atgatatgaa agaaaaatta ttaggtacta
     6661 ttatttggag tattgctaca ttttattatt caagaatgat ggaaataatg aatttagcta
     6721 ttttaaaaat aaaaattggg ggaagttaat atgctaaaca ttcaagacgt tagtcatctt
     6781 tctaaaaagg agcaaaaagc atataaccgt ttcgtagaat ctgtagaaaa cggtaattta
     6841 ccagtactac catgtattga aatggatcta aaagagatgc aagaagaaac attaaaccag
     6901 agtaagattg gtggaatgcc atttttaaaa tattttaaag atataccatt agatgaaaat
     6961 aatgtaccaa tggtattgct agcacagatt aatttggatg atcttccaga acaacaagaa
     7021 ttatttcctg taaaagaagg gatattgcag ttttggatta gttcagaaga tcaaatgtat
     7081 ggaatgtctg aaaatttaaa gggaaacaat ataaactcaa ggcttgttta tataaaagag
     7141 ccaattacag atttatcact cgaaaatatt caagcgcatt tgaagtcatt agatgctgat
     7201 aatgaggata tcccgttcag tggagcattt tctatagaat ttagattgtc gaaacaaact
     7261 attacatgta ctgattataa gtacgatgag gacgtgcttg cattgtggaa taaagtcaat
     7321 ccatccttcg cgctaaaatc aatgtttggt ggttatgatg aattgatgga acctgtgtgt
     7381 aacacattta ctgctaagga accatttaat caacttggtg gttatccata ttttgaccaa
     7441 atagatccaa gaacgaacga tcaagaactg aaaatgtatg atagagtctt actgcaaatt
     7501 gattctacaa gagatggtaa ttcttcgatt atatggggtg atttaggtat tgccaatatc
     7561 ttagtgaaat ctactgacct tgaggctatg aagtttgatg attacatgta ttcatgggat
     7621 tgcagctaag agaatcataa aaatatatta ctttaataag tgtagtatct atagatcaat
     7681 ctagggagtg ggacataaat gatattttcg caaaatttat ttcgtcgtcc caccccaact
     7741 tgcattgtct gtagaaattg ggaatccaat ttctctttgt tggggcccat ccccaacttg
     7801 cattgcctgt agaatttctt ttcgaaattc tctgtgttgg ggcccctgac tagaattgaa
     7861 aaaag
//
LOCUS       DAMQYT010000117         7820 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMQYT010000117 DAMQYT010000000
VERSION     DAMQYT010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.117"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            224..1399
                     /gene="rpsA"
                     /locus_tag="PGA33_001874"
     CDS             224..1399
                     /gene="rpsA"
                     /locus_tag="PGA33_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDG6473678.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
     gene            1621..2931
                     /gene="der"
                     /locus_tag="PGA33_001875"
     CDS             1621..2931
                     /gene="der"
                     /locus_tag="PGA33_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDG6473679.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEAYVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTGAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            2948..3946
                     /locus_tag="PGA33_001876"
     CDS             2948..3946
                     /locus_tag="PGA33_001876"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDG6473680.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            4117..4389
                     /locus_tag="PGA33_001877"
     CDS             4117..4389
                     /locus_tag="PGA33_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDG6473681.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
     gene            4820..5392
                     /locus_tag="PGA33_001878"
     CDS             4820..5392
                     /locus_tag="PGA33_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HDG6473682.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDLYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            5395..6120
                     /locus_tag="PGA33_001879"
     CDS             5395..6120
                     /locus_tag="PGA33_001879"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HDG6473683.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            6122..7081
                     /locus_tag="PGA33_001880"
     CDS             6122..7081
                     /locus_tag="PGA33_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDG6473684.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            7173..7622
                     /gene="ndk"
                     /locus_tag="PGA33_001881"
     CDS             7173..7622
                     /gene="ndk"
                     /locus_tag="PGA33_001881"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDG6473685.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
BASE COUNT         2859 a         1031 c         1514 g         2416 t
ORIGIN      
        1 tgctaatttt tatacaaaaa atatgtataa acatagcaaa ctgtattact ttgatacaaa
       61 aatggttgta ataaatattt atcgatatga cgacttgaat atgataaagt gacatattta
      121 tgtatatgac tatttcgcaa aatgtaatcg aggtagaatt tcttgacaat tctgtcagtt
      181 tataagatgt tataaatatg tagtgtataa ggaggcaaac aagatgactg aagaattcaa
      241 tgaatcaatg attaacgata ttaaagaagg tgacaaagtc actggcgagg tacaacaagt
      301 tgaagacaag caagttgttg ttcatatcaa cggtggtaaa tttaatggga ttattcctat
      361 tagtcaatta tctacgcatc atattgatag cccaagtgaa gttgtaaaag agggcgacga
      421 agttgaagca tatgtcacta aagttgagtt tgatgaagaa aatgaaactg gagcttacat
      481 cttatctaga agacaacttg aaactgagaa gtcttatagt tatttacaag aaaaattaga
      541 taataatgaa atcatcgaag cgaaagtaac agaagtagtt aaaggtggtt tggttgttga
      601 tgtaggacaa agaggttttg ttccggcttc actaatttca acagacttca ttgaggattt
      661 ctctgtgttt gatggacaaa caattcgtat taaagttgaa gaattggatc ctgaaaataa
      721 tagagtcatt ttaagccgta aagcagttga acaagaagaa aacgatgcta aaaaagatca
      781 attattacaa tctttaaatg aaggcgatgt tattgatggt aaagtagcgc gtttaactca
      841 atttggtgca tttatagaca ttggcggtgt tgatggttta gtgcatgtat ctgaactttc
      901 tcacgaacat gttcaaacac cagaagaagt agtttcaatt ggtcaagatg ttaaagttaa
      961 aattaaatct attgatagag atacagaacg tatttcatta tcaatcaaag atacgttacc
     1021 aacacctttc gaaaatatta aaggtcaatt ccacgaaaat gatgtcattg aaggtgtcgt
     1081 agtaagattg gcaaactttg gtgcatttgt tgaaattgca ccaggtgtac aaggacttgt
     1141 acatatttct gaaattgcac acaaacacat tggtacgcca ggtgaagtgt tagaacctgg
     1201 tcaacaagta aatgttaaaa tattaggtat tgatgaagag aatgaaagag tatcactatc
     1261 tattaaagca acattaccaa acgaagatgt tgttgaaagt gatccttcta cgactaaggc
     1321 gtacttagaa aacgaagaag aagataatcc aacaattggc gatatgattg gtgataaact
     1381 taaaaatctt aaactataat ttaatattta atagtcaact ccacatgttt atgattgcat
     1441 gtggagtatt tttatgtaac aaaatatact cggaatgata acgtggacaa atttaactaa
     1501 gtgtttaaaa agataaagtt ttaagtgctg atttttatca ttacagtaat aaactcattt
     1561 tgaatacaca gtctcatgtg atattattaa aaagatataa gaaagagagg aagttagctt
     1621 atgactaaac ctatagtagc tattgtaggt aggcctaatg taggtaaatc tacaattttt
     1681 aatagaatag ttggagaacg tgtttcgatt gtggaagaca cgccaggtgt aacacgagat
     1741 cgtatttatt cttcaggtga atggttaaca catgatttca atattattga tacaggtggt
     1801 attgaaattg gtgatgcacc attccaaaca caaattagag cgcaggcaga aatcgccata
     1861 gatgaagcgt atgttattat ttttatggtt aacgtgcgtg aaggattgac acaaagcgat
     1921 gaaatggtcg ctcaaatttt atacaaatct aaaaaaccgg tcgtattagc ggttaacaaa
     1981 gtagataata tggaaatgcg tacagacgtg tatgatttct attcattagg atttggtgaa
     2041 ccgtatccga tatcagggtc acatggttta ggtcttggtg acttgttaga tgcagttgtt
     2101 tctcattttg gtgaagagga agaagatcct tatgatgaag atacaattcg actatccatt
     2161 attggacgac caaacgtagg taaatcaagt ttagtaaatg ctattttagg tgaagatcgc
     2221 gttatcgttt ctaatgttgc agggacaacg agagacgcta ttgatacaga gtatagttat
     2281 gatggacaag attatgtttt aatcgatact gctggtatgc gtaaaaaagg aaaagtatat
     2341 gaatcaactg agaaatattc agtattaaga gctttaaaag cgattgaacg ttcaaatgtt
     2401 gttttagtgg tcatagatgc agaacaaggc atcattgaac aagataaacg tgttgcagga
     2461 tatgcacatg aacaaggtaa agcagtcgtg attgtcgtaa ataaatggga tactgtggaa
     2521 aaagatagta aaacgatgaa gaaatttgaa gatgaagtac gtaaagaatt ccaattttta
     2581 gattatgcac aaattgcttt tgtgtctgct aaagaacgca caagattacg tacattattc
     2641 ccttacatca atgaagcaag tgaaaaccat aaaaaacgtg ttcaaagttc aactttaaat
     2701 gaagttgtta ctggtgcaat ttccatgaac cctacaccaa cagacaaagg tagacgtttg
     2761 aatgtctttt atgcaacaca agttgctata gaaccaccga catttgttgt atttgttaat
     2821 gatgtagaat taatgcattt ttcttataaa cgctatttag agaatcaaat ccgtgccgct
     2881 tttggttttg aaggtacacc aattcatatt atagctcgaa agagaaatta acgattgggg
     2941 gataacaatg actaaaatta ccgtttttgg tatgggaagt tttgggacag cccttgccaa
     3001 tgttcttgca gaaaatggac atgatgtttt gatgtggggt aaaaatcaag atgctgttga
     3061 tgaattaaat acatgtcata caaataaaaa gtatttaaaa tacgcgaaat tagatgttaa
     3121 catcatcgct acttcagata tgaccaaagc aattcaattt gcagatattt acttaatggc
     3181 tttacctact aaagcaatgc gagaagttgc ttctcaaatt aatgataagc tgacctctaa
     3241 aaagactttt atacatgttg ctaaaggtat tgaaaatggg acgtttaaac gtgtgtcaga
     3301 aatgattgaa gattctattt cacctgaata taatgcaggt attggcgtgt tgtcagggcc
     3361 aagtcatgcg gaagaagttg tagtcaagca accaactaca gttgctgctt catcaaaaga
     3421 taaaagtgta agtaaattaa cgcaagattt atttatgaat gattatttgc gtgtgtacac
     3481 gaatgatgac ttgattggtg ttgaacttgg tggtgcattg aaaaatatca tcgcagtagc
     3541 aagtggtatc gtagctggaa ttggctacgg tgataatgca aaagctgcat taatgactcg
     3601 tggcttagcg gaaattagta gattaggtga aaagttaggt gccgatccta tgacatttct
     3661 aggtttaggt ggtatcggtg acttaatcgt tacttgcaca tcaacacatt ctcggaattt
     3721 cacattagga tataaacttg gacaaggtga atcaatggat caagcattat ctgaaatgaa
     3781 tatggttgtt gaaggtattt atacaactaa atcagtttat catttagcta aagaaaaaaa
     3841 tgtggatatg ccaattacaa atgcattata tagagtatta tttgaaaata tctcagtaaa
     3901 agaatgcgta aaagatttaa tggagcgcga taaaaaatct gaataaaatg tgtattatta
     3961 cacatatacg gctaataatt acgtaaaatg aatagaaatg gcttaaattc aacgtttttg
     4021 ccacataaat gattgcgatt tatgtaatgc ttgtgttaaa gttttaccat aatgatataa
     4081 acatatcatt atcaaacctc aggaggtgaa tgtctaatga acaaaacaga tttaatcaat
     4141 gcagttgcag agcaagctga tttaactaaa aaagaagctg gttcagcagt agatgctgta
     4201 ttcgaatcaa tccaaaactc acttgctaaa ggtgaaaaag tacaattaat tggtttcggt
     4261 aactttgagg tacgtgaacg tgctgcacgt aaaggtcgta accctcaaac tggtaaagaa
     4321 attgatatcc cagcaagtaa agttccagca ttcaaagctg gtaaagcatt aaaagatgct
     4381 gtaaaataat tttacttaaa aagccctgaa taagggcttt ttattttgct tttaatactt
     4441 acaacctgta cataaattgt aatgttcttc taagttttta atctttggaa cattcattgt
     4501 ttaagcgttc aaaatattac atgacataat agttttgata aaaatagcaa ctacaaaata
     4561 tacatatgaa tattgagatt aattgttagc gttgaattta cttaaaaggt aaccatgtct
     4621 actatagtat tttacgttat ttaaaaagat gaataatgta aatgaagtaa aggttattat
     4681 gagaattaca aaagctacat aaattactgt tagtttaaat tgaaatttaa aaatgataag
     4741 taatcttaat gcttcaaaag attttaattt cttacatata atgttaatat gaatagtgat
     4801 aatatcgtga ggtgtagaca tggaaacaac tgttagcaaa ttggaaagac aaatagaaga
     4861 aagattaaag ggcgtatcag aatatgaatc cattaatatt aatcatcggt taggtaagtt
     4921 acttgattta tatgatattc cagatgttgc caaagtggca tgcctcacaa ttgacacatc
     4981 aatgagacac cttgatgaca ttacatataa tcatttgtct aaacattcca ttttgattgg
     5041 agatttaatt agtgcacatt tttatacgct actagcagaa atcaatgatt tatcatttca
     5101 aaatgaaatt agtaaagcaa ttgttgaaat taatgaattg aaatcttcat tacatcatca
     5161 agctttaaat gattatgaaa tttctcaagc aatagtaaaa attgaaacac ttttcccata
     5221 tattacgtta tctcatttcg gtatcaatat tgatgagtcg gaaatatata attatttatt
     5281 tgaagatatg tctgattact atccatcata cttcaagaaa tataatcaaa gtgaagtaaa
     5341 gcattactta cacgatatcc aaaaaagtta tttaaaaagt agaggtaatt aagcatggct
     5401 gacaataaag caaataaaga gcaagtacat cgcgtttttc aaaatatttc aaaaaaatat
     5461 gatagattaa ataatattat tagttttgag cagcataaag tatggagaaa acgcgtcatg
     5521 aaagacatgg gtgttagaaa agggacgaaa gcattagatg tttgttgtgg tactggtgat
     5581 tggacaatcg cattaagcaa agctgtagga ccaacaggtg aagttactgg tattgacttt
     5641 agtgagaata tgttagaagt aggaaaagaa aaaactgctt caatggaaaa tgttaaactt
     5701 gttcatggtg atgcaatgga attgccgttt gaagacaatt cttttgatta tgtaacaatt
     5761 gggtttggat taagaaatgt gccagactat ttagtcgcgt taaaagaaat gaatagagta
     5821 cttaaaccag gtggtatggt ggtatgtctt gaaacgagcc aaccaacttt gccagtcttt
     5881 aaacaaatgt atgcactata ctttaaattt gttatgccta tttttggaaa attatttgca
     5941 aaatcaaaag aagaatatga atggttacag caatctacgt ttaattttcc tggaaaagaa
     6001 gagttgaagc gcatgtttga agaagcaggt ttcataaatg taagagtacg tagttttaca
     6061 gggggcgttg ctgcaatgca ccttggctat aaagaaaaag ataataccaa aggtgattaa
     6121 cgtggcaaag ttaaacatga acaatgaaat taagaaagtg gaacaacgac ttgaaaaggc
     6181 aataaaaagt aaagattctg tattagaaca ggcatcatta cacttattgt cttctggtgg
     6241 taaaagagta cgcccagcat ttgttattct gagtagccaa tttggcaaag atgaacaaac
     6301 gtctgaacaa acgtatcaag ttgcagtcgc attagagtta attcatatgg caacacttgt
     6361 tcatgatgac gttattgata aaagcgacaa gcgtcgaggc aagttaacca tatcaaagaa
     6421 atgggatcag acaactgcta ttttaactgg gaatttttta ttggcattag gacttgaaca
     6481 cttaatggcc gttaaagata atcgtgtaca tcaattgata tctgaatcta tcgttgatgt
     6541 ttgtagaggg gaacttttcc aatttcaaga ccaatttaac agtcaacaga caattattaa
     6601 ttatttacga cgtatcaatc gcaaaacagc actgttaatt caaatatcaa ctgaagttgg
     6661 tgcaattact tctcaatctg ataaagagac tgtacgaaaa ttgaaaatga ttggtcatta
     6721 tataggtatg agcttccaaa tcattgatga tgtattagac ttcacaagta ccgaaaagaa
     6781 attaggtaag ccggtcggaa gtgatttgct taatggtcat attacgttac cgattttatt
     6841 agaaatgcgt aaaaatccag acttcaaatt gaaaatcgaa cagttacgtc gtgatagtga
     6901 acgcaaagaa tttgaagaat gtatccaaat cattagaaaa tctgacagca tcgatgaggc
     6961 taaggcagta agttcgaagt atttaagtaa agctttgaat ttgatttctg agttaccaga
     7021 tggacatccg aaatcactac ttttaagttt gacgaaaaaa atgggttcaa gaaacacgta
     7081 gtatttatga aaagtattga aagcgcttta ccaacctgtt aatatataat agtaatatac
     7141 ataaacctat taaacacagg gggatacata aagtggaacg tacattttta atgattaaac
     7201 cagatgcagt acaaagaaat ctaattggtg aagtaatttc aagaattgaa agaaaaggac
     7261 taaaacttgt cggtggtaaa ttaatgcaag taccaatgga acttgctgaa acacattatg
     7321 gtgaacacca aggtaaacca ttttataatg atttaatttc atttattaca tcagcaccag
     7381 tgttcgcaat ggtagttgaa ggtgaagatg cagttaatgt atctagacat attattggca
     7441 gcaccaatcc ttcagaagct tcaccaggat caattagagg tgatttaggt ttaactgttg
     7501 gtagaaatat cattcacggt tcagattcat tagagtctgc tgaacgtgaa attaatctat
     7561 ggtttaatga aaatgaaatt actagctatg cttcaccacg tgatgcatgg ttatatgaat
     7621 aaaatataaa ctgtaaacct ttacgattta tttataaagg tagaaagggt tttgttatgt
     7681 ggttagtcat tatgattata cataacaagg cccgtttttt atgttgtagt aaattacttg
     7741 aaaaatttta tagttttttg gtaacacgta ttaaaaagag aggaatattc tttatcaaat
     7801 gaaactaaac agagagaagg
//
LOCUS       DAMQYT010000118         7794 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMQYT010000118 DAMQYT010000000
VERSION     DAMQYT010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.118"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            362..1282
                     /gene="mvk"
                     /locus_tag="PGA33_001882"
     CDS             362..1282
                     /gene="mvk"
                     /locus_tag="PGA33_001882"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDG6473686.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            1287..2270
                     /gene="mvaD"
                     /locus_tag="PGA33_001883"
     CDS             1287..2270
                     /gene="mvaD"
                     /locus_tag="PGA33_001883"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDG6473687.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            2283..3359
                     /locus_tag="PGA33_001884"
     CDS             2283..3359
                     /locus_tag="PGA33_001884"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDG6473688.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEETDQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            3536..3877
                     /locus_tag="PGA33_001885"
     CDS             3536..3877
                     /locus_tag="PGA33_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDG6473689.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKVEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(3929..5251)
                     /locus_tag="PGA33_001886"
     CDS             complement(3929..5251)
                     /locus_tag="PGA33_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDG6473690.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSPKMYGGTCI
                     NIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHDDIVDSITAPHIIINTGATSVIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAYGITDLENKGIAL
                     HTNVETTELSSDNHHTTVHTNVDNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(5238..5678)
                     /locus_tag="PGA33_001887"
     CDS             complement(5238..5678)
                     /locus_tag="PGA33_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDG6473691.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMIDTIRGKDGGYLANDQSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYNGKTIKDVIEDIQKEDLCHENI"
     gene            5736..5837
                     /locus_tag="PGA33_001888"
     CDS             5736..5837
                     /locus_tag="PGA33_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473692.1"
                     /translation="MCQYLCLKSVELGVVIVMIYCLKVDALTVRLHN"
     gene            6303..7712
                     /locus_tag="PGA33_001889"
     CDS             6303..7712
                     /locus_tag="PGA33_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDG6473693.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSAGTAVGAGLATFVLGSNPVGWV
                     ILAGLAMSTVFALGTDLIYQNNIFGLKDKVDWVGHKIDNSIDVVKKTTEKSMDSVGNA
                     VSEAKNIISNHINPMKWAW"
BASE COUNT         2759 a         1094 c         1531 g         2410 t
ORIGIN      
        1 gaccttctat tgaaatgttc taatacttta gtagtaaatc tatatattcc aattattatt
       61 tgtagtatta tagttagggt ggaaaattat aaattgtagg gctattttat aaaaagataa
      121 agaatctgaa atatcactcg ataagattta aactttgaag taaacgaaag ttagttttat
      181 actaatcaag ttaagtcaaa tataaattca aaataaagat tatacataat atgaataatg
      241 tgagataaag actgcaatga aatttaacga gaatgcgtat gcaacttaga atatgatgtc
      301 ttttactaat aaataaaaat aatgaatcaa gtataggata aatataaaag gtgatattga
      361 catgacaaga aaaggatatg gggaatcgac aggtaagatt attttaatag gagaacatgc
      421 tgttacattt ggagagcctg ctattgcagt accgtttaac gcaggtaaaa tcaaagtttt
      481 aatagaagcc ttagagagcg ggaactattc gtctattaaa agcgatgttt acgatggtat
      541 gttatatgat gcgcctgacc atcttaagtc tttggtgaac cgttttgtag aattaaataa
      601 tattacagag ccgctagcag taacgatcca aacgaattta ccaccatcac gtggattagg
      661 atcgagtgca gctgtcgcgg ttgcttttgt tcgtgcaagt tatgattttt tagggaaatc
      721 attaacgaaa gaagaactca ttgaaaaggc taattgggca gagcaaattg cacatggtaa
      781 accaagtggt attgatacgc aaacgattgt atcaggcaaa ccagtttggt tccaaaaagg
      841 tcatgctgaa acgttgaaaa cgttaagttt agacggctat atggttgtta tagatactgg
      901 tgtgaaaggt tcaacaagac aagcagtaga agatgttcat aaactttgtg aggaccctca
      961 gtacatgtca catgtaaaac atatcggtaa gttagtttta cgtgcgagtg atgtgattga
     1021 acatcataac tttgaagcct tagcggatat ttttaatgaa tgtcatgcgg atttaaaggc
     1081 gttgacagtt agtcatgata aaatagaaca attaatgaaa attggtaaag aaaatggtgc
     1141 gattgctgga aaacttactg gcgctggtcg tggtggaagt atgttattgc ttgccaaaga
     1201 tttaccaaca gcgaaaaata ttgtaaaagc tgtagaaaaa gctggtgcag cacatacttg
     1261 gattgagaat ttaggaggtt aatgcgttga ttaaaagtgg caaagcacgt gcacatacga
     1321 atattgcact tataaaatat tggggtaaaa aagatgaagc actaatcatt ccaatgaata
     1381 atagcatatc tgttacatta gaaaaatttt acactgaaac gaaagtcact tttaacgacc
     1441 agttaacaca ggatcaattt tggttgaatg gtgaaaaggt tagtggcaaa gaattagaga
     1501 aaatttcaaa atatatggat attgtcagaa atagagctgg catcgattgg tatgcagaaa
     1561 ttgaaagcga caattttgta ccaacagcag cagggttggc ttcatcggca agcgcatatg
     1621 cagctttagc agcagcttgt aatcaagcgc tagacatgca gctgtcagat aaggatttat
     1681 cgagattggc gcgaattggt tcgggttctg cgtcgcgtag tatttatggt ggatttgcag
     1741 aatgggaaaa agggtatagt gatgagacgt catatgccgt tccacttgaa tcgaatcatt
     1801 ttgaagatga ccttgccatg atatttgttg tgattaatca acattctaaa aaggtaccta
     1861 gtcgatatgg tatgtcattg acacgaaaca catcaaggtt ttatcaatat tggttagatc
     1921 atattgatga agatttagct gaagcaaaag cagcgattca agacaaagat tttaaacgcc
     1981 ttggtgaagt aattgaagaa aatggtttgc gtatgcatgc cacgaatcta ggatcaacac
     2041 cgccgttcac atatcttgtg caagaaagtt atgatgtcat ggcgcttgtt cacgaatgcc
     2101 gagaagcggg gtatccgtgt tattttacaa tggatgcggg acctaatgtg aaaatacttg
     2161 tagaaaagaa aaacaagcaa cagattatag ataaattatt aacacagttt gataataacc
     2221 aaattattga tagtgacatt attgccacag gaattgaaat aattgagtaa ggaagagata
     2281 aaatgattca ggtcaaagca cccggaaaac tttatattgc tggagaatat gctgtaacag
     2341 aaccaggata taaatctgta cttattgcgt tagatcgttt tgtaactgct actattgaag
     2401 aaacagacca atataaaggt accattcatt caaaagcatt acatcataac ccagttacat
     2461 ttagtagaga tgaagatagt attgtcattt cagatccaca tgcagcaaaa caattaaatt
     2521 atgtggtcac agctattgaa atatttgaac aatacgcgaa aagttgcgat atagcgatga
     2581 agcattttca tctgactatt gatagtaatt tagatgattc aaatggtcat aaatatggat
     2641 taggttcaag tgcagcagta cttgtgtcag ttataaaagt attaaatgaa ttttatgata
     2701 tgaagttatc taatttatac atttataaac tagcagtgat tgcaaatatg aagttacaaa
     2761 gtttaagttc atgcggagat attgctgtga gtgtatatag tggatggcta gcgtatagta
     2821 cttttgatca tgaatgggtt aagcatcaaa ttgaagatac tacggttgaa gaagttttaa
     2881 tcaaaaactg gcctggattg cacatcgaac cattacaagc acctgaaaat atggaagtac
     2941 ttatcggttg gactggctca ccggcgtcat caccacactt tgttagcgaa gtgaaacgtt
     3001 tgaaatcaga tccttcattt tacggtgact tcttagaaga ttcacatcgt tgtgttgaaa
     3061 aacttattca tgcttttaaa acaaataaca ttaaaggtgt gcaaaagatg gtgcgtcaga
     3121 atcgtacaat tattcaacgt atggataaag aagctacagt tgatatagaa actgaaaagc
     3181 taaaatattt gtgtgatatt gctgaaaagt atcacggcgc atctaaaaca tcaggcgctg
     3241 gtggtggaga ctgtggtatt acaattatca ataaagatgt agataaagaa aaaatttatg
     3301 atgaatggac aaaacatggt attaaaccat taaaatttaa tatttatcat gggcaataaa
     3361 tgatttgata ggggagcaat catctaattt aattagaaga taaatgctcc attttttatt
     3421 gtcagccgtg ttacgtatct tgaaaaaaat attaaaaaga gtaaaataga tagagttaac
     3481 gaaaaaatta atgaaatcga cgatatagat atgataaaga aaggtgggta gcaatatgaa
     3541 aaatacattc cttatttgtg atgaatgtca ggcagtcaat ataagaacgt tacaaaagaa
     3601 gttggaaaaa ttagatcccg atgctgaaat cgtgataggt tgtcaatctt attgtggacc
     3661 tggacgccga aaaacattca cttttgttaa taaccgccca ctggctgcgc ttactgaaga
     3721 agaattaatc gaaaaagttt ctcaacaatt aaagaaacca cgtgatcctg aagaagaaga
     3781 gcgtttaaga aaacgacatg aagaacgtaa acgtcgtaaa gtagaacaag atagaaagct
     3841 taaagaaaaa ttagaaaagc gaaaagcaca acaataaagc ctgatggcag catcattcaa
     3901 tgcgtgccac caggttttta tgttttgtct agaaattaaa taaatcatta aatgattcgg
     3961 ccatcgtagg atgcgtataa atattatctc gtaatacggt atatggaatg ttttgatcaa
     4021 tcgcaagttt aattatatta attaattctt cagattgctt accatataat gtagcaccta
     4081 aaatcatatt attttcatta ttaatgacta ctttaaataa acctcttgga tcattgttaa
     4141 ttttatgacg aggtatagca cttactaaaa gttgatgttc agtgtaatca taatgttgag
     4201 cggcagcttc tttactagtt aatccaacac gtgataatgg tggatctata aatactgtat
     4261 aaggcacgct gcctctattg tcagtcgtac gtgactgatt accatataac gctgatttga
     4321 taattcgata atcatctaaa gatatatacg taaattgaag tccgccttta acatcacctg
     4381 cagcataaat atgcggcaca gttgtttgaa gatgagcatt gactttaatt tcgcctctgt
     4441 cgcctaattc gatatcagta ttttctaaag ctaaatccgt attcggtttg cgcccgatag
     4501 ccaaaagtac tgcatcagcc tcaaagttat caacgttggt atggactgtt gtatgatgat
     4561 tgtcagatga caattcagtc gtttcaacat ttgtatgcaa tgcaatgcct ttattttcta
     4621 agtcagtaat accatatgca acgacatctt gatcttcgcg tggcataaat gattcgccac
     4681 gttctaatac tgttacctta ctacctaaat tcgcaaacat tgaagcaaat tctaaggcga
     4741 tataaccgcc acctacaata acgaggtgct taggttgata gctaatgttt aataaacctg
     4801 tcgaatcgaa gacgtgttta gcttgatcaa ggcctttaat gttaggaatg acagaggtag
     4861 caccggtatt aataatgata tgaggtgcag taatactatc gacgatatcg tcatgttgat
     4921 ctaataaatt cacttcagta ttagatttaa actgcgcttt aaaatccagt acatcaatgt
     4981 tgttatcgtc tgctaataag tggtaatttt tattgtttag cgcattgaca acatcgtttt
     5041 tacggttata acttgcttca aaagatttgc cttctaatcc atcatgtaca agtgtcttcg
     5101 aaggtataca tcctatgttt atacaagtgc ctccatacat tttcggagat tgttcgataa
     5161 ctgcgacgtg ttgacctgtt gatgcagcgt atttcgctaa agttttacca gctttcccaa
     5221 atcctattac aattaaatca tatgttttca tgacataaat cctccttttg aatgtcttca
     5281 atgacatctt tgattgtttt tccattataa aaattaataa tgatattctg ttcgtcttgc
     5341 tgataatgtg acatggtagt tgcaatatta cgagcaattt gacagtgact gccttcgtcg
     5401 ccagtaaata gacgtgtgtg gtgttctttc tctaagacaa aatgtttata taatgttgct
     5461 agagagacat cagcactttg atcatttgct aaataaccgc catctttacc tcgtattgtg
     5521 tcaatcattt ttaaatcgac aagttgagtc gtcacgcgtc gtaattgaac aggatttaaa
     5581 caagttaatt ctgctaatga actactattg aatttttctg aatgatgctt agttaaaaaa
     5641 gctaatacat gcacggcaat gttaaattct aaattcaatg tttctcacct cataattgta
     5701 actgtattag ttacaattaa aaatcatttt gctcaatgtg tcaatactta tgtttgaaaa
     5761 gtgttgaatt aggtgtggtt attgtgatga tttattgttt gaaagttgat gcattaacgg
     5821 tgagattgca caattgaaat tttaattatg tatgaactga tagatatgaa atgaatattg
     5881 aaaatgagag caataaaatt ttgaatgatg atgttgtgtg attgtataaa cttattcgta
     5941 ttttagatac gttttgatat ttcgcatgat gaggaaatac ttttaacaac tgatgacata
     6001 ttttaatagt gaatatattt tgagatatta atatatttaa tgttaaggat gttgtcgcaa
     6061 taaaattaaa atattgaaag ataattttta ttgattaatt gcgaatatac aaagacacca
     6121 ttatatccat atgctaaaat aagaaaaaag gcgacatgtt atctcaattt acctgcatct
     6181 agactccttt acataagaaa taatcatcgc cgagttttaa aattttacaa ctttgtagat
     6241 gtaaaaagca tttgatttta aatagtttga aaatcatata tttttgaaaa aggagcatgc
     6301 cgatgagtat tgacatgtat ttagacagat ctcgaaacca agcttcaagt gtggggaatt
     6361 tgagtcaaac aatgaattca aattatgatg cgttggaaaa agcaattact caatttatta
     6421 atgatgatgc gcttaaaggg aaagcgtata cgtcagctaa gcaatttttt agtacggtgt
     6481 taattccatt atcaacaagt atgaaaacat tgagtgattt aacgaagcaa gcttgcgata
     6541 attttgtgtc acgttatacg agtgaggttg atagcatatc tttaaaagaa tcagagcttg
     6601 aagaagatat cagatcatta agtcaacaaa ttacgcgata tgaaaatttg aataacaatt
     6661 tgaaaaagca tgcttccgat aatcagcaag ccatttcatc gaaccaacaa ataatacgaa
     6721 cattaggtca acaaaaacat gaattagaag agaagctacg caaattgcgt gagtttaatc
     6781 aaaaatcacc agaaatattt aaagaagttg aagaatttca aaaaattgtc caacaaggac
     6841 ttacccaagc gcagaatttt tggaactttt caacaaatca atttaatatt ccttcaggta
     6901 aagaacttga ttgggccaaa gcaagtcatg aaaaatattt gaaagttgct atggggaaaa
     6961 ttgaacataa agcagagaaa gaaactttaa ataaagcaga ctttgctgtt ataaaggcat
     7021 atgccaaaga acatccagaa gacgatatcc cgaaaagtat attgaaatat ataaatgaca
     7081 ataaagacag tattaaaaga gatataggat tagatattac gtcaacactt ttagagcaag
     7141 gcggtataaa tgcaagtaaa ttcggtgtat ttatcaatac agcaggtgga gtgaaaggcc
     7201 cagcaggtcc aaattcattt gtggaagtca aacgtacatc aggtaatgtg tttatagaaa
     7261 atggtagtaa atttgcaaaa ggcggaaaat acctaggtaa aggtgttgct ggtgtaggat
     7321 ttggtatagg tatgtatgat gaccttgcaa atgatgataa aacatttgga gaggcgttgt
     7381 cgcataatgg tatgacgctt gcagctggat ctgcagggac agcagttgga gctggattag
     7441 cgacttttgt tttaggaagt aatccagtag gatgggtgat tttagcaggt ttggctatga
     7501 gtacagtatt tgcattagga acagatttaa tttatcaaaa taatattttt ggattaaaag
     7561 ataaagtaga ctgggtaggg cataaaatcg ataatagtat agatgttgtt aaaaaaacta
     7621 cagaaaaatc catggatagt gttgggaatg ctgttagtga agctaaaaat attattagca
     7681 atcatataaa tccaatgaaa tgggcgtggt gagttttgct ttttaatatt aaggttatta
     7741 ataaaaatcc gagatataaa gtcgttcaat ataatgatga gtatttgtta attg
//
LOCUS       DAMQYT010000119         7749 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMQYT010000119 DAMQYT010000000
VERSION     DAMQYT010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.119"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            157..1779
                     /locus_tag="PGA33_001890"
     CDS             157..1779
                     /locus_tag="PGA33_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDG6473694.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            2043..2729
                     /locus_tag="PGA33_001891"
     CDS             2043..2729
                     /locus_tag="PGA33_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDG6473695.1"
                     /translation="MTNQFKNKQSKLHDSLESITKNLYATPTSELPFDNRFLFKSFIL
                     KRETGNIVIYHSGHLGDSQQDIASLGGVSKVLMNHDHESIGGSNQVEAPYFIHENDVA
                     ALKHKISVQKQFSNRVMLDKDLEVIPAPGHTPGTTLFLWDDGHHRYLFTGDFICFEGK
                     RWRTVILGSSDREKSIQSLEMVKELDFDVLVPWVTIKDEPLVYFVENEYEKREQIQNI
                     IDRVREGENS"
     gene            complement(2917..3480)
                     /locus_tag="PGA33_001892"
     CDS             complement(2917..3480)
                     /locus_tag="PGA33_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HDG6473696.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINSLLESSDLTEAETQEAQKIKSQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(3569..3664)
                     /locus_tag="PGA33_001893"
     CDS             complement(3569..3664)
                     /locus_tag="PGA33_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473697.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            3733..5223
                     /gene="betB"
                     /locus_tag="PGA33_001894"
     CDS             3733..5223
                     /gene="betB"
                     /locus_tag="PGA33_001894"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDG6473698.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            5484..7193
                     /gene="betA"
                     /locus_tag="PGA33_001895"
     CDS             5484..7193
                     /gene="betA"
                     /locus_tag="PGA33_001895"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDG6473699.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            7471..7683
                     /locus_tag="PGA33_001896"
     CDS             7471..7683
                     /locus_tag="PGA33_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDG6473700.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
BASE COUNT         2587 a         1164 c         1531 g         2467 t
ORIGIN      
        1 tatataaata ttcaataatt gccaagtttc aaattgtatg aaatttgcat tattattaaa
       61 tgttagttat tgtcaatttt gtgaatcaat ataattatta cattttgaga taaatcgaaa
      121 caggattcat aaaattaata attaggggga gcacaattga aaaaagagaa agttatggac
      181 tggacgacct ttatagggac agtagctgta cttctttttg cagttatacc tatgatggct
      241 tttccaaaag caagtgaaga tatcatcact ggtattaata gtgccatttc tgattcaatt
      301 ggttcgatat atttatttat ggggctggcg attttttgct ttgtaatgta tatcgcattt
      361 ggtaaatatg gtaatgtcac acttggtaaa gcaagtgata aaccagaatt taatacattt
      421 acatgggcgg caatgctgtt ttgtgcaggc ataggctctg atattttata ctggggcgtt
      481 attgaatggg ctttttacta tcaagttcca ccaaatggcg cgaaaagtat gagtgatgaa
      541 gcactccaat atgcgacgca atatggtatg ttccactggg ggccaattgc ttgggctatt
      601 tatgttctac cagcattacc aattggttat ttagtatttg ttaaaaaaca accggtgtat
      661 aaaattagtc aagcttgtcg tccgatttta aaaggtcaaa cagataaatt tgtaggtaaa
      721 gttgtagata tcttatttat ctttggattg ctaggtggtg cggcaacatc actagcgtta
      781 ggtgtgccat taatttctgc aggcatagaa agattaactg gtttagatgg taaaaatatg
      841 attttacgtt cggccatttt attaacaatc acggttatat ttgccattag ttcatataca
      901 ggattgaaaa aaggtattca aaagttaagt gatatcaacg tttggctatc ctttgtactt
      961 ttagccttta tatttattat tggaccgact gtttttatta tggaaacgac agtgacaggg
     1021 ttcggaaata tgttgagaga tttctttcat atggcaacat ggttagaacc attcggtggt
     1081 attaaaggtc gaaaagaaac gaatttccca caagactgga caatattcta ctggtcatgg
     1141 tggttagtat atgcgccatt tatcggttta tttatcgcta gaatttcaaa aggtcgacgc
     1201 cttaaagaag tcgtgctagg aacaattatt tatggaacgc ttggatgcgt attattcttt
     1261 ggtatttttg gtaactatgc tgtgtattta caaatttctg gacagtttaa tgtaacacaa
     1321 tatttaaata cacatggtac agaggcaacc attattgaag tggtgcatca tttaccattc
     1381 ccatcattga tgattgtact attcttagta tctgctttct tattcttagc aacaacattt
     1441 gattcgggtt catatatttt agcggcagca tctcagaaaa aagtggtagg cgaaccatta
     1501 cgtgccaatc gtttattctg ggcatttgca ttgtgcttat tgccattttc attgatgcta
     1561 gttggtggtg aacgtgcatt agaagtattg aaaactgctt caatactggc aagtgtgcca
     1621 ttaattgtta tttttatttt catgatgata tcatttttaa tcattttagg gcgcgataga
     1681 attaaacttg aaacgcgtgc tgaaaaatta aaagaagttg aacgtcgttc attgcgaatc
     1741 gttcaagtat cagaagaaga acaagacgat aatttataat tcaaagcggg tctgggacga
     1801 cgaaatgaat tttgtgaaaa tatcatttct gttccattcc ccttttttta gtagcattgt
     1861 aggatgaact tttaggtttt cattaatgtt gtactaaaag atttaatttt ttagtgctcc
     1921 aagtacttat ttattgtatg aagcatattc taaatcgaag tttgaaagac tctcattgat
     1981 tattaaatta aataaagggt actgcgtatg tacaattcaa attaatcgaa ggatgaaata
     2041 aaatgactaa tcaatttaaa aataaacagt ccaaattaca tgacagttta gaatccatca
     2101 caaaaaactt atatgcgaca cctacttcgg aactgccttt tgataatcga tttctattta
     2161 aatcttttat tttaaaacgt gaaacaggta atattgttat ctatcattcc ggacacttag
     2221 gtgactccca acaagacatt gcatcattag gtggtgtttc aaaagtattg atgaatcatg
     2281 atcatgaatc tataggaggt tctaatcaag ttgaagcccc ttactttata catgaaaatg
     2341 atgtggctgc actgaaacat aagatttctg ttcaaaaaca atttagtaat cgtgtaatgt
     2401 tggataagga tttagaagtt attcccgcgc ctggacatac accagggacg acactatttt
     2461 tatgggatga tggtcatcac cgttacttat ttactggaga ttttatatgt tttgaaggga
     2521 agagatggcg tacagttata ttaggttcaa gtgatagaga aaaatctatt caaagtttag
     2581 agatggttaa agaattagat tttgatgtac ttgtaccttg ggttactatc aaagatgaac
     2641 cgttagttta ttttgtagaa aatgaatatg aaaaacgtga acaaatacaa aatattattg
     2701 atagagtacg tgagggcgag aatagctaat tgaaatatat tggcgaagca atgtaacgaa
     2761 tctaagaaag ccctagaaaa tacctccata attgattgtc atataaaaca aaaacggtaa
     2821 tttctattta ttgagataga aattaccgtt tatttcgtgg acctattgca ttgtttttat
     2881 catgcataat catcattgtc gttgtttgag tcaattttaa ttttcagaat cagaaggctg
     2941 ttctggaatt gggaaatatt tgaaaatttc accgctttca atcgcttcgg ttaactgttc
     3001 taaccattcg taataaacat gtgtatgatc aagctgagat ttaatttttt gtgcctcttg
     3061 tgtttcagct tcagttaaat cactgctttc aagtaatgaa ttgataatag cttgagcatc
     3121 ttttactgct tcgacattga tgtcaatttc acgctggaat tttttagtga aaaagtttcg
     3181 gaaaaagatg aaaaagtctt tctcggcgat aaaatgttgt ttgcggcttc ctctcgtaaa
     3241 ttgttgttta acaatatcaa attcctgcaa tttcttaacg ccagcactca tacttggttt
     3301 gctcatttgc aattgatgac gcatttcatc aagcgtcata ctgccttcaa acaccattgt
     3361 gccatataag tttcctacac ttctattagt gccatacaaa tccattgtct gtccaattga
     3421 attaattaca atatcttttg cttgttctaa ttgttgctgt ttgttctgag aacgagtcat
     3481 cattgcacct ccgtacatca ttttggtcac gttaaaataa atactaatac attataaaac
     3541 cttttctaaa aaaagacatt aaaaatattt aaagcattaa agttaaatgt ttcgttaaat
     3601 aaaaatctaa cgaacttaca aaacttaatt cttgagttgt tttgtaaatt gacacatttt
     3661 tcatttctat gctaacataa gtttgtaaaa ttcgttaaat aaaaatttaa caaacttaac
     3721 ggaggttgtt gaatggaact tttaaaacat ttatctcagc gtcaatatat tgatggtgag
     3781 tgggttgaaa gcgcgaataa aaatacaaga gatattatca atccttacaa tcaagaagtg
     3841 atatttacgg tttctgaagg gacaaaagag gatgcagaac gtgcaatctt agctgcaaga
     3901 cgtgcgtttg agtctggtga atggtcgcaa gaaactgctg aaacaagagg taaaaaagta
     3961 cgtgccatcg cggacaagat taaagaacat cgcgaagcgt tagcacgatt agaaacatta
     4021 gatactggaa aaacgttaga agaatcatat gcagatatgg atgatattca taatgtgttt
     4081 atgtattttg ctggattagc agataaagac ggtggcgaaa tgattgattc accaattcca
     4141 gatacagaaa gcaaaattgt taaagaacca gtaggtgtag ttacacaaat tacaccttgg
     4201 aattatccgt tattacaagc atcatggaaa attgcgccag cgcttgctac gggttgttca
     4261 ctagttatga aaccaagtga aattacacca ttaacaacaa tacgtgtttt tgaattaatg
     4321 gaagaagttg gtttccctaa aggaacaatt aatcttattc taggtgcagg ttctgaagtt
     4381 ggtgacgtaa tgtcaggtca taaagaggtt gaccttgtat catttacagg tggcattgag
     4441 actggtaagc atattatgaa aaatgctgct aataatgtta cgaatattgc cttggaactt
     4501 ggcggtaaaa atccaaacat tatctttgat gatgctgatt ttgaattggc agtagaccaa
     4561 gcgttaaatg gtggatattt ccatgcaggt caagtttgtt cagcaggatc aagaatatta
     4621 gtacaaaaca gtattaaaga caaatttgag caagcactta ttgatcgcgt gaaaaaaatc
     4681 aaattaggta atggttttga tgctgatact gaaatgggac cagtgatttc aacagaacat
     4741 cgtaataaga tcgaatctta tatggatgta gctaaagcag aaggcgcaac aattgctgtt
     4801 ggtggtaaac gtccagatag agatgattta aaagatggtc tattcttcga gccaacagtc
     4861 attacaaatt gtgatacgtc aatgcgtatt gtacaagaag aggttttcgg acctgtcgtt
     4921 actgtagaag gctttgaaac tgaacaagaa gcgattcaat tagcgaatga ttctatatat
     4981 ggtttagcag gtgctgtatt ttctaaagat attggaaaag cacaacgcgt tgctaacaag
     5041 ttgaaacttg gaacggtgtg gattaatgat ttccatccat attttgcaca agcgccatgg
     5101 ggtggataca aacaatcagg tatcggtaga gaattaggca aagaaggctt agaagagtac
     5161 cttgtttcaa aacacatttt aacaaataca aatccacaat tagtgaattg gtttagcaaa
     5221 taaaaattag ataaggtgag tgccattgta agaacacaag acactcactt tgttttgtat
     5281 aagtggcgaa atgttgattg ataatttgga ctaaacgcaa aatgaatcat agattatttc
     5341 attactgtta gtaacaatcg taaaaggaaa agcgagtgtt ttggttagct aagtttagca
     5401 attcaacgat aaccaatcag ccactaacaa atatttcatg caatactcac tttgaaatac
     5461 aacaaacttt ggaggtcata acgatgagta acaaaaacaa atcatatgat tatgtcatca
     5521 ttggaggagg cagtgcaggt tctgtactag gtaatcgtct gagtgaagat aaagataaag
     5581 aagtcttagt attagaagcg ggtcgcagtg attatttttg ggatttattt atccaaatgc
     5641 ctgctgcgtt aatgttccct tcaggcaata aattttacga ttggatttat tcaacagatg
     5701 aagaaccaca tatgggcggt cgtaaagtag cacatgcccg aggtaaagtt ttaggtggat
     5761 caagttcgat taatggcatg atttatcaac gtggtaatcc aatggactat gaaggctggg
     5821 cagaaccaga aggtatggaa acttgggatt ttgcgcactg tttaccgtat tttaaaaaat
     5881 tagaaaaaac atacggtgca gcgccttatg ataaatttag aggccatgat ggaccaatta
     5941 agttaaaacg agggccagca acgaatcctt tattccagtc attctttgat gcaggtgttg
     6001 aagcaggcta tcataaaaca cctgatgtga atggatttag acaagaaggt tttggaccgt
     6061 tcgatagtca agtacatcgt ggtcgccgaa tgtcagcttc aagagcatat ttacatccag
     6121 cgatgaagcg taaaaactta accgttgaaa cacgtgcctt tgtaactgaa attcattatg
     6181 aaggtagaag agcaactggt gttacgtata agaaaaatgg caaactacat accatcgatg
     6241 ctaatgaagt cattttgtct ggtggggcat tcaatacgcc acaattacta caattatctg
     6301 gtatcggtga ttcagagttc ctaaaatcaa aaggcattga gccacgtgtt catttacctg
     6361 gtgtgggtga aaactttgaa gatcacttag aggtatacat tcaacataaa tgtaaggaac
     6421 ctgtatcatt acagccaagc ttagatatca agcgcatgcc atttattggt ttacaatgga
     6481 ttttcacacg tacaggtgca gcagcatcta accattttga aggtggcgga tttgtacgtt
     6541 caaataacga agttgattat cctaacttaa tgttccattt cttaccaata gctgtaagat
     6601 atgatggtca aaaggcagcg gtcgcacatg gttatcaagt tcacgtagga ccaatgtatt
     6661 caaactctcg tggtagcttg aaaatcaaat ctaaagatcc attcgaaaaa ccaagtatcc
     6721 gctttaatta tttatcaaca gaagaagata aaaaagaatg ggtagaagca attcgtgtag
     6781 caagaaatat cttatctcaa aaagcaatgg atccattcaa tggtggagaa atttcaccag
     6841 gaccagaagt gcaaacagac gaagaaattc ttgattgggt tcgtagagat ggtgaaacag
     6901 cattacaccc atcttgtagt gctaaaatgg gtcctgcttc agatccaatg gcagtagttg
     6961 acccgttaac aatgaaagtc cacggtatgg aaaatttacg tgtagttgat gcatcagcta
     7021 tgccacgtac gacaaatggc aatatacacg cacctgtttt aatgttagct gaaaaagcag
     7081 cagacattat acgtggcaga aagcctttag agccacaata tattgattat tataagcatg
     7141 gtgttcatga tgaaaatgaa ggtgcaatcg aagtaaaacc atacgctaaa taaatttata
     7201 tagtagactc ttagtctggc atctattgta ttggtgccag gcttttttgc gttttatcta
     7261 aatgagaagt taatgtgttg ttcaaaaacg gtgtgtgagt atatgaactg aaaatagagt
     7321 taattgtaca ttgtgatgat taattgacct aattggcaga cattaaaaga gtgcacaacc
     7381 ttaaaaagtt cactaaatag acatttaatt gtgttttctt gaaagctttc gtggtaggat
     7441 ttatataagt aaattgagag aaggaatact atgagctttt acgaatatat acaaacattt
     7501 aaagatgata aaacaccatt aggcgaatta gcgatttgga ttaaagaaga tgattcattc
     7561 ccaaaacaag agaagctaac agaaaacatt ttgtcttatt ttcatcaaat gtccaatata
     7621 gatcatgagt ttttagaaat tgtaaaaaga tcactttctc tgtatgatca attaaaatcg
     7681 taaaatcaga aataaagtta gctaaaataa aaggccggtg atttcacaga tgaagttgtc
     7741 ggctatttt
//
LOCUS       DAMQYT010000120         7671 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMQYT010000120 DAMQYT010000000
VERSION     DAMQYT010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7671
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.120"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(268..465)
                     /locus_tag="PGA33_001897"
     CDS             complement(268..465)
                     /locus_tag="PGA33_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473701.1"
                     /translation="MNCYDEIFNTIKELIENKEISSYQINKDTGISYGNINAMRRRER
                     RIENLSLKNAKILYEYAKKVL"
     gene            complement(958..2298)
                     /gene="glnA"
                     /locus_tag="PGA33_001898"
     CDS             complement(958..2298)
                     /gene="glnA"
                     /locus_tag="PGA33_001898"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDG6473702.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            complement(2317..2685)
                     /locus_tag="PGA33_001899"
     CDS             complement(2317..2685)
                     /locus_tag="PGA33_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDG6473703.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            complement(2928..4166)
                     /locus_tag="PGA33_001900"
     CDS             complement(2928..4166)
                     /locus_tag="PGA33_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDG6473704.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEKVITRLKNVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFETKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
     gene            complement(4185..5423)
                     /gene="hflX"
                     /locus_tag="PGA33_001901"
     CDS             complement(4185..5423)
                     /gene="hflX"
                     /locus_tag="PGA33_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDG6473705.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            5535..6011
                     /locus_tag="PGA33_001902"
     CDS             5535..6011
                     /locus_tag="PGA33_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDG6473706.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            complement(6233..6466)
                     /gene="hfq"
                     /locus_tag="PGA33_001903"
     CDS             complement(6233..6466)
                     /gene="hfq"
                     /locus_tag="PGA33_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDG6473707.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
     gene            complement(6481..7416)
                     /gene="miaA"
                     /locus_tag="PGA33_001904"
     CDS             complement(6481..7416)
                     /gene="miaA"
                     /locus_tag="PGA33_001904"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDG6473708.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            complement(7434..>7671)
                     /locus_tag="PGA33_001905"
     CDS             complement(7434..>7671)
                     /locus_tag="PGA33_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDG6473709.1"
                     /translation="NMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVK
                     VNLYHHKRHEVLFEKDHDKIWEDLFKWLNQFYKK"
BASE COUNT         2375 a         1405 c         1070 g         2821 t
ORIGIN      
        1 aagtgcacct aaaggttttt atgtaatgta ttaaatattt ccatttatac atatacatct
       61 tgtataaaaa gaaagaactc catatatact caaaaggtat actgaagctc tatatattat
      121 ataaaattat tatactattt tgaaaaacat aagtaaatca ctaaacatga tttctttcac
      181 ttttagaaaa cttttaatac tataaaagca cccactcagt cactagtttg ggcagttatt
      241 gtatgcctat tgaactcaat gcttatatta caataccttt ttcgcatatt catataagat
      301 ctttgcattc tttaagctta aattttctat tcttctttct ctacggcgca tagcattaat
      361 attaccgtaa cttatcccag tatctttatt aatttgataa ctcgatatct ctttgttttc
      421 tatcaattct ttgattgtat tgaatatttc atcatagcaa ttcataaatt agatgaggcg
      481 aaatttttaa ttttttagaa tatcaatagt agtataacta aaatgaaaat accgatcgat
      541 aaacaaaaag atattttttg ttttgtttct cttttcatat agtattaccc ccttaataat
      601 gcgtagtaag gtccctcttt tcggggtctt accttagaac gttctgcaaa tgaattcgat
      661 gagaagtaat atgaatatgg ctattttcaa gtaatactca acgttttcgc gacgttcttt
      721 tatcgcctca tctcatcacc tccaaatata ttaaaattca tgtgaactaa aatataaaat
      781 ggtcttcccc agctttaaaa aaataaatac ataaaacatt tacttgacca aaacttgacc
      841 aaatccattt tttaacaaaa gtggtcaagt gaaatcccat ctttccaaat caaaaaagag
      901 ctaaagcaaa atgctctagc ccttgatatt actgatttcc catcattgtt tagcgtatta
      961 atattgcttc atgtactgat ctctttccca ttcagagact tgagttctgt agtaatccca
     1021 ttcaattgat tttgaattaa taaattgatt atagatatga tttcctaaag cttttttaat
     1081 aacttcattt tcacgcattg cttttaatgc agtataaagt gttgaaggta agtcttgaat
     1141 gcctactgct tcacgttctt cacggttcat ttcgtaaata ttttggttaa ctggttctgg
     1201 aacttttaat ttatttttaa taccatctag tccagcttct aagattgcag ctaacgccat
     1261 gtatgggttt gcagctggat ctactgaacg tacttcgata cgagtagata atcctcttga
     1321 agatggtaca cggattaatg gtgaacggtt tttaccactc catgcaatat aacatggtgc
     1381 ttcataacca ggtactaaac gtttatatga gtttactaac gggttacata cagcagtaaa
     1441 tccgcgtgca tttttaagca cacctgctgt aaattgatat gcagtttccg ttaagcccat
     1501 ttcagtattt ggatcaaaga atgcattttc tttacctttg aataatgaaa cgttaaagtg
     1561 cataccgcta ccattcacac cgaataatgg tttaggcata aatgttgcgt gtaaattatg
     1621 tttacgtgcg attgttttaa caaccaattt aaatgtttgg atattatcac atgctgtaac
     1681 agcatctgca tatttaaagt caatttcatg ttgaccaggg gcaacttcat ggtgactagc
     1741 ttcaatatcg aagcccatat cctctaattc taaaacaata tcacgacgac agttttcacc
     1801 taaatctgta ggtgctaaat cgaaatatcc accatcatca ttaagttcta aagttggttc
     1861 ccctttttca tccaacttaa acaagaagaa ttctggttca ggccctaggt taaagtctgt
     1921 gaagcctaaa tcttccattt cttttaatac acgttttaag tttgcacgag gatccccttc
     1981 aaatggtgtt ccatctgttt tatatacatc acaaattaaa cgtgcaactt taccttgtcc
     2041 agcagtccat gggaagatta cccaagtatc taaatctgga tgtaagtaca tatctgattc
     2101 ttcgatacgt acgaaacctt cgatagaaga accgtcaaac atcatttcgt tatcaagtac
     2161 tttttctaat tggcttacag gcacttcaac atttttaatt gttcctaaaa tatcagtgaa
     2221 ttgtaatctt aaatatctta cattttcctc ttctgcaaat ttacgaatgt cgtctttagt
     2281 gaaagtacgt tttggcattt taaaatcctc caaattttat ttaataaatc gggataaatc
     2341 accacgattt attggcaaag tttctccaat aggcttttgc gtggcatcta caatcatctt
     2401 ttttcttatc tcttgttcat ctgttgttaa atgctcttgt gagtcataaa tgatttgttt
     2461 aatccctttg atattaaatc ctttttctaa taatgattta atttctagta atctttccaa
     2521 atcattgagt gagaacagac gtttttgacc ttctgttctt tcaggtttga tgagttcatg
     2581 tgtttcatag taacgtattt gccttggcgt taaatccgtt aacttactta ctacactcat
     2641 agagaagaca gccatatttc gtctgattgc atcattcgat atcattgttc ctctcctcta
     2701 cttttgaact tgttaataac ttatcataat ttttttagga tttcaaaaat atgtcaggtt
     2761 ttctaacata aagcttaaaa cattgatata actgcgattt cgacttctaa aacgacataa
     2821 aacaatctac agctaaaata ttttttcaat ttttaaaaat aatctgataa atctccgcaa
     2881 aaaatttcac ccaaaacaca ttatcattta gggtgaaatt taatctatta tataagtcct
     2941 tgttctttca actgattaac agctcttgtc acagcaattt taacgtgttc atatgttaat
     3001 cctccttgaa catatgcttc ataaggagga cgaataggtc catctgcaga taattcaatc
     3061 gatgaacctt gaataaacgt accagctgcc ataataacat catcttcgta accaggcata
     3121 taactaggtt ctggactaaa atgtgcatta attggggatg cgtgttgaat actttgacaa
     3181 aatgaaatca tttgttcttt cgtttcaaat ttaactgttt gaattaaatc agttcgtttt
     3241 tcgtagtact tcggcgttgt gttcatattc attttttcta aaaataaact agtaaacaat
     3301 gcacctttaa gactctgact gacaacgtgt ggcgctaaaa agaaaccttg atacatttca
     3361 agcaatgcat ttaatgatgc acccgcttct ttaccaatac caggtgctgt caatctataa
     3421 ccacatcgtt caattaaatc ttttctacca gcaatgtatc caccaatctt agctaaacca
     3481 ccgccagggt ttttaattaa tgatcctgct attaaatcgg caccacattc tataggttca
     3541 cgtctttcaa caaattcccc ataacagtta tccacaaata ttaaaatatt aggatgcacg
     3601 tttttcaacc tagtaattac cttttcaatt tcatctagcg gaattgaagg tctttgatca
     3661 tagcctttcg aacgttgaat cgctattact ttggtgcgct cagaaacccc atctaacaca
     3721 ctttcaatat cgatcttacc ttctttaagt gcaatatctt tatacgatac gccatgctcc
     3781 attaaacttt caataccatt tccgtttacg ccaatgactt caagtaaagt gtcatatgga
     3841 ctacccgtta tataaattaa ttcatcacca tgttttaata aactttgtaa tgcaatagta
     3901 atcgcatgcg tacctgaaat aatttgcgga cgaacaattg catcttctgc tttaaatgcc
     3961 tgcgcatata tttcttctaa atgatcacgt ccaaagtcat catacccgta tcctgttgat
     4021 ccttgtagat cactttcggt tgctttgaca tgatgaaatg catttaatac tttttcttga
     4081 ttaatatatg ctgtttcttc aatttcttta aaatatggtg ctaacgttga ttcgacgtca
     4141 gctactatct tacttatatc tttcatttga atttcgtcct tttattattt tttaaatcct
     4201 tttatacgat aaacattttc aatttcatca tatttaagtt cagtcactaa tgtatgttgt
     4261 tttagaaaat ataatcgatc tgcattattc gtcgcaattg tctcatcata ataagttaat
     4321 tgccttttga tttcatcaat aaataacgtc ttaacaagta atttatcacc atcattttta
     4381 gaagaaacaa aaacattagg caaatcactt gctggacgat ttgatgcatg atcacataag
     4441 tcctttttat taaaaataac tatttgagaa atatgactca tatctaattg tttgattaaa
     4501 tcattaactg tgtcatactg cgtacggtat tcaggatggc tactatctac gacatgtact
     4561 aataaatctg cacctttagc ctcttctaaa gttgatttaa aagctgcaat taacgtcgta
     4621 ggtagtttct gtataaaacc aacagtatct gaaataatta aattaaatcc atcatttatt
     4681 tgaatttgtc gtgttttagg atctaacgtt gcaaataatt gatctttttc atacgtctct
     4741 tcatttgcta aaacattaaa ccatgatgat ttaccagcat ttgtataacc aactaaagct
     4801 acttgaaaca cctgattttg atttctttta tttcgatatc tttcgcgatg ttcttctacc
     4861 gtccgcaatt gatgtttaat ttcattcata cgagttcgaa tatgtctgcg atccatctct
     4921 aacttcgttt caccagggcc tctagttcca ataccgccac ctaaacgaga aaggctttta
     4981 ccatggcctt gcaatctagg taataaataa tcaagttgtg ctagctctac ttgcaattta
     5041 ccttctttac ttcttgctct taatgcaaat atttcaagaa tcaactgagt tctatcaata
     5101 atttttacac ctaaagcttc atttagtgat ttggattgtg cagtcgttaa ttcatcattt
     5161 gtgatgacta catcaatatc tttgaactca ataaatgctt gaatttcttc aattttacct
     5221 ttaccaacat aatatttgcg atctacacga tctctgtttt gagtaatttg acccaacact
     5281 tcaagttggc aagtctctga taaagatgat aattcctcca ttgtagactc aaaattaaat
     5341 tgcttatcat cttgagcatg tacaccgact aagacagctt tttctagttt atttttagta
     5401 tcatgaattt gttgctgagc catgttatac atctccttaa taaaatccta ctcaaagtat
     5461 tctatcatag agttttagta aagactatta cacgattttg tggtacattt taagtaaatc
     5521 gaaaggatga tacaatggag acaatttatg attttgtagt agaaacaaat aaaggtgtta
     5581 cttacaaatt agatgcatat aagggtgacg ttatgctaat tgttaataca gctagcgaat
     5641 gtggctttac ttcacaattt gaaggtttgc aatcattata cgagaaatac aaagatcaag
     5701 ggtttgtaat tctaggtttc ccttgtaatc aatttggtgg ccaagaacct ggttcaggcg
     5761 aagaagcagc tcaaaactgt aaattaaact atggtgttac cttccctatg catcaaaaaa
     5821 tagacgtaaa aggggaacat caattacctt tatttagata tttgactgcg gcacaacacg
     5881 gattctttaa tgagaaaatc aaatggaatt tcactaagtt tttagtggat cgcgaaggta
     5941 acgttgttaa acgatttgca cctcaaaaaa aacctgttca aatcgaaaga gaaatagaaa
     6001 agttattata aaatatacta gctcatagca aataaaacgc ttcatataaa gtcgtatcat
     6061 atattgactc gacctttata tgaagcgttt ttattacaaa cattcctcta ttgtatcttg
     6121 tcatttgaac atcatttaat tgatgtcaca tatgacttta tttctatatc tatttatcat
     6181 acttcaaacg ttatgtacac gagtcattaa atcgtgccca tatgattaca acttattctt
     6241 cactttcagt agatgcttga ccttcagttt ctactgtata agtgctgatc gcatgtttgt
     6301 aaatcaagtg ttgtttgcct tgagaattta agcttacgac atacttgtcg tattcttcaa
     6361 taacaccttt catttggaaa ccgtttagaa agaatacagt tacttcagtt tggtttgctt
     6421 taaaattctc tagtgctttg tcttggatgt tttcgtttgc aatcatctgt cggactcctt
     6481 ttacttaatc tgggttgtaa tctcatctaa catcatttga agtgacatat tttctttatc
     6541 taaccaatga acactcattt tattcttgaa ccatgtcatt tgtcgttttg catattggcg
     6601 tgaatgttgc tttaaatcat tgacagcatc ttcataaatc atttgtccgt taatcacagg
     6661 tattaattct ttatatccaa tagcttgcat actttggcaa gattcatagc cttgttcaac
     6721 aagttgttgc acttctctaa ataatccgtg atccaacata atatcaacac gtttatttat
     6781 tcttgaatat aatgttttac gcgacatttc aatccctaat aataatgtat cataattttc
     6841 agtaaattgt tgcactttct tgcgattact caaaagtttt tttgttttta aataatattc
     6901 aatagcgcgc aacactcttt ggcggttgtt agggtgaata ttttctgcag aaaccgcatc
     6961 aaattgagct aaataatcgt gtagttgctg attatctaaa tgttctaatg cagataactt
     7021 ttgtttaact atggataatt gtgcaggtgt aactgtttca tcttctaatt cataattata
     7081 tattaatgat tgaatatata agcctgttcc acctgctatg attggaactt tacctctatt
     7141 cgttatatca gtaattaaat cttctgctaa tcgcttgaat tcatatgctg aaaatgtatc
     7201 atcaggattc aagatatcaa ttaaatgatg tggaatacca tccatttctt caggtgttac
     7261 ttttgcagtt ccaatattca tatgtttgta gacttgcata gagtcaccgc ttatgatttc
     7321 accattgata cgcttcgcga gttcgatgct aagctctgtt ttacctgaag cagttggccc
     7381 cacaattact acaataaaag gcttattttt attcatattt aattccactt tctttatttt
     7441 ttataaaatt gattcaacca tttaaacaag tcttcccaaa ttttgtcatg atctttttca
     7501 aataacactt catgacgttt atgatgataa agattcactt taacattttt aatgccagct
     7561 tttctatata tattcgccaa ttttaaaatc cctttaccat aatcacctaa aggatcgtca
     7621 tatcctgaaa ctagtaatat aggcatggca tgatttaaca ttttcatatt t
//
LOCUS       DAMQYT010000121         7651 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMQYT010000121 DAMQYT010000000
VERSION     DAMQYT010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.121"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..709)
                     /locus_tag="PGA33_001906"
     CDS             complement(<1..709)
                     /locus_tag="PGA33_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDG6473710.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     AQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILL"
     gene            complement(975..1955)
                     /gene="czrB"
                     /locus_tag="PGA33_001907"
     CDS             complement(975..1955)
                     /gene="czrB"
                     /locus_tag="PGA33_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HDG6473711.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
     gene            complement(1957..2277)
                     /gene="czrA"
                     /locus_tag="PGA33_001908"
     CDS             complement(1957..2277)
                     /gene="czrA"
                     /locus_tag="PGA33_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HDG6473712.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            2429..3094
                     /locus_tag="PGA33_001909"
     CDS             2429..3094
                     /locus_tag="PGA33_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDG6473713.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGDQDIKDALTQFEN
                     ETD"
     gene            3529..4467
                     /locus_tag="PGA33_001910"
     CDS             3529..4467
                     /locus_tag="PGA33_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HDG6473714.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            4498..4728
                     /locus_tag="PGA33_001911"
     CDS             4498..4728
                     /locus_tag="PGA33_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473715.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            5400..6773
                     /locus_tag="PGA33_001912"
     CDS             5400..6773
                     /locus_tag="PGA33_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HDG6473716.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRISNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
     gene            7231..7644
                     /locus_tag="PGA33_001913"
     CDS             7231..7644
                     /locus_tag="PGA33_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HDG6473717.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
BASE COUNT         2797 a         1037 c         1367 g         2450 t
ORIGIN      
        1 ataatagtat tccaatgaaa aatttgaaaa atgttttttg accatttttc attgctttaa
       61 atccattagc taagtgtgtt tttactttat cgttagttga ttcaacgaaa ctagtcataa
      121 agttgaaaat aggtattaac aataaccaag taataattgc tttaatcaat aaaacaacaa
      181 tgattatgat gctttgagtt gtaatagcaa taccaatcag gtgtgtacta tctgcatatg
      241 atgattgtac tgaattcata atcatttctg atactggaga aaataatttg tatagtaatg
      301 atgtcagaag tgaaagaacg ataatcattg ctatagatac aagaccgatc agaacgctct
      361 ttaaataatt acctttttta aatgcaataa ataaatcggt aaattttact ttttcatgac
      421 tcattgctcg tttcataaca tttgtaattc cgataaaaat ttgaactaaa acaaacagtg
      481 aaactacagc tgctataagt aatacagcga taacttttaa atatgcatct actggtggtt
      541 tttgcccaaa ttgtgaataa attgcaatag tttgtgcata cttagcaagc tggaaattag
      601 caagtaaata tacaactgca attacagcaa atgctataag tgcatatatc aatgttatta
      661 ataattgtgg tttagcattt ttaaatgctg atttaaagta agtaaacaaa gtggtgcctc
      721 cttttcttca tataaataca ataacaaaaa acacgttatt ttcatattaa tatctaataa
      781 aaattaatta aagtgactaa agttagagaa ttataatagt aaattgtcaa aaatatgact
      841 aatgtaagaa ataatagttg aaaatttgtt aataaacatt gcatcatcaa agaccaaaca
      901 agtcgccata aagacataaa aaacctactg ccattgaaaa gtttaatcga tgcaatggca
      961 gtaggttaaa attattaatg tacatgcgcg tgatgatgag catgatggtt atgtgaatga
     1021 ctatgtgttc ctgaacatat aatcgattca tcatgtttgt gattaggcgt ttctaattga
     1081 atagtcatat ggtgaatatt taaatgtaat aaatcatgct caatgttttc taataataat
     1141 tcacattctt tcattgtcaa tgtatggtct acaacaacat gacaactcaa tgcattcata
     1201 tcatttgaaa ttgtccaaac atggcaatca tgcacacttt gtattcgtga atccttttta
     1261 atagtagtta taacttcatc tatatcaaca tcacttggtg tgccttccat taaaatgtta
     1321 attgaagatt ttgtgatacc ccaagcactt tttaaaataa taacggaaac taaaatactt
     1381 gcgataggat cggcgattgt ccatccaaat gcccaaatta aaatagctgc agtaatggcg
     1441 ccaactgaac ctaataagtc tccgataaca tgtagaaaag caccacgcat atttaaattg
     1501 tgtgaagtgt cgccgccttt aaacataaag aatgcaacaa cgatattgac aattaaaccg
     1561 ataatactaa tgattaacat ttcttttgat tgaacttcag aaggaacaaa gaaacgttta
     1621 atcgcttcaa aaacaatcaa aatacttatt acaaaaagcg ttacaccgtt aaataacgct
     1681 gcgagtactt cgaaacgttt ataaccaaat gtttttgtag ttgtggcatt cttttcagca
     1741 taaataaatg cgacaagtgc aacacctaat gagaatgtgt cgctaaacat atggataccg
     1801 tcagatagta atgccaagct gttagcaagg agaccgccga tgatttcgat aaacatatat
     1861 agaccgatta ttaaaaacga tataaacaat actttcttat tatttgtagt tacatgacta
     1921 tgcatatggt catgatgatg atgtgaatga gacatattat aacccacttt ctttaggatg
     1981 attcgcgtga tgtatggctt gctttaacat agttgctacg tggatgtcat ctaatgaata
     2041 aatcattgat tggccttgtc gttttgcttt cacaagatgc acacttttaa gtaattttaa
     2101 ttggtgcgag acatttgatt gagataaatt caattgatgt gaaatgtgac caacacttgc
     2161 ttcgctgact gataacaatt ccatgatacg tattcgattg taatcgccta atgccttgaa
     2221 aatttcagtt acgcgttcta atgtatctgt atttatttct gaatattgtt ctgacattat
     2281 atgacaatcc tttcatgtga atatttgttc atatattaat ttaatacgtt cccagtagta
     2341 tgtcaattga tttttatagt tgtaacaagt tttacatatc tcataaagtg gtatggcata
     2401 gagaataata aataggagag tgttgtaaat gaatattttg gttataggtg ctaatggcgg
     2461 tgtaggttcg ttactagtgc aacaattagc aaaagaaaat gtaccattta ctgctggtgt
     2521 taggcaatca gatcaactta atgcgttaaa atcacaaggt atgaaagcaa ttcttgttga
     2581 tgttgaaaat gattcaatag agactttaac tgagacgttt aaaccattcg ataaagttat
     2641 cttttcagta ggttctggag ggaacacggg tgcagataaa acaattattg tcgatttaga
     2701 tggtgctgtt aaatcaatga ttgcgagtaa agaggccaat attaaacact atgtcatggt
     2761 ttcaacatat gattcgagac gtcaggcttt tgatgatagc ggtgatttaa agccatatac
     2821 aatagctaaa cattacgctg atgactattt aagacgttca ggtttgaatt atacaatttt
     2881 acatccaggg gcacttacaa atgcagcagg atctggaaag atagaagctg cacaatattt
     2941 tgatggtaaa ggtgaaattc ctagagaaga tgttgcgaca gtattaaaag aaattgtcac
     3001 atccaatcat tttaatcacc aagagtttca aattattagt ggtgatcagg acattaaaga
     3061 tgctttaact caatttgaaa atgaaacgga ttaataagga tagtaaatat gatttgaagc
     3121 gggttacttt ttcgtaactc gttatttttt gaaaatgatg aattataagt atgaataagt
     3181 acgtattgtc tatgcgtttt acaaagaatt tgaacaatgt atggcacaac tattaaagtt
     3241 gtttattaat tggtataatg ggcgagtaaa tttaaaggga aatatgtaca aatgtaagag
     3301 agatataatc tctatgctat aaatttttga aaatgttaaa gtactgtaaa tttaagtgaa
     3361 gcgctttaat tggcagtact gcaatgttag taaatatggt acaatcacta atagttaatt
     3421 tgaaatgtgg aagttaataa tacgatgtaa atgagaacaa aatcaaaata taatggaaca
     3481 acaacatcag agttctattg cttacttaat cacaatggag gtgcaacaat gccattattt
     3541 ttacaaccaa ttttaaaaac aaaattatgg ggcggtcaac gtctaagtga gtttggatat
     3601 caattagaca atgatacaac tggggaatgt tggtgtgtgt cagcacatcc aaatggtacg
     3661 agcgagatta ttaatggacc atatcaaggt caaacattag accgtatttg gtcagaacat
     3721 cgtgaattgt ttggtgattt cccaagcaaa gattttccgc ttctaactaa aatagtggat
     3781 gcaagagaat cactttctat tcatgtgcac cctgataatt cttatgctta tgagcatgaa
     3841 aacgggcaat atggcaaatc tgaatgttgg tatattatag atgcagaaga agatgcagaa
     3901 atagttatag ggacattagc agagtctaga gaagaagttg cgaatcatgt tcaacacgga
     3961 acgatagagt cgatacttag atatattaaa gtaaaacctg gagaattcta ttttattcca
     4021 gcaggaacag tacatactat ttcttcagga atattagcat acgaaacgat gcaatcgtca
     4081 gacattacat atagacttta tgatttcaat cgtcaagata atcaatataa tgatagaccg
     4141 ttaaatattg aaaaagcttt agacgttatt cagtacaatg caccattacc taatattttg
     4201 cctgaaagcg aaattattga aaaccataag tgtacacaca ttgtatcgaa tgatttcttt
     4261 acattggtta aatgggaaat ttctggcacg ttaaattata tgaagcctag agagttctgt
     4321 ttagttacag tgttggaagg cgaagggcaa atgattgtcg atggtgaaat tttcaaactg
     4381 actactggta caaactttat tttgacttct gaagatttgg atagtgtctt tgaaggtgat
     4441 ttcacattga tgattagcta tgtgtaatag tgtaataaca atttaggagt gaatgatttg
     4501 aagcaatttc tatatattgc gttagtatgt ggtgtgatag caggtcttgg tgctttctta
     4561 catataccgc agtatccgag catgacaatt ccacgtatag tagctatttt aggaattatc
     4621 agtgctatgt tgacttttaa agacaagcaa atcagcgcct cattaaagtt tagcgcattg
     4681 ttaattaatg tgctgccatt atgcggtacc tttgtagctt caaattaatt gtcgatagcg
     4741 cgtttgaata taatacagtg cattaacaga agaaatatga ttgtgttaat gacattgatg
     4801 aaaataaagc acatcaacat aacctctcac ttgcatagta gtgagggggt ttttgttggt
     4861 ttatataaaa tgaagtaatt aatatttaat gaaaattaag acatgataaa attctaacgg
     4921 cagtatagat atttaaaaat gtgtatcaat ggcgctatag agataagcaa gcatagtggt
     4981 atcatatata aatttgtaat ttttattgca tagtgtatgc aagcatgttt tttatatgca
     5041 aaataggtag ctaaatatgt taaactgttt gtaacaattt ttatcattaa gacagatata
     5101 ataaataaaa tatagaaaac tgattaaaag gtatacagtt gctcgtatgt gtgtccaact
     5161 gtatttagag tcgcacttgt tgtaaagcaa aatagttcga cttacttaga agtgaaaacg
     5221 ataagggtct gggacgtaaa ttaatgtcct gggcgctaca atgttatagg aaatgcgatt
     5281 aaaatcgcag tgaacaaagg gtatacagtt gttcgcatac gcgtccaact gcataagaaa
     5341 cactaaaaga atgtaattca tagtgtttct aaaatttatt caggttaagg agtattaaga
     5401 tgacagcaga aacgaattat ttttggttga attgtggata taaccgttgg aatcataatg
     5461 aaccgttagt aggacaaaca gctttatttg aatcaggcgc acattttaac ccatctcaag
     5521 gatttagagc gtttaaaaag gctaaagtag gagatcaagt catcttttat caagttcaga
     5581 cggatactgg actacttgga tgtggtgaaa ttattagtgt tgaaacaggt gcacaaaata
     5641 aaatacgtgt acagtttcgt tttaatgaac aattgaaacc attaacagca gattatttaa
     5701 agcgtagtga agctttggaa tttcgtataa gtaatatgaa agagacattg ttcaatcaaa
     5761 tcactgcaga agagtttgat ttaataagtg gtcttggtaa aggcgaaatt aagataccgc
     5821 gttatttctt tttagctgaa actgaagaat ttgaacctgg aaaccaatat acgatttata
     5881 cgcacacgta caacggtatt aaacgtaatg gttatcattt ttatactcag ttagaagaag
     5941 gcgacaatat tattttttat aatcgaacga aaaatcaatc tgtcgttgga attggagaag
     6001 tatcaaagca tattcatgaa aaaccaccaa ttcctggtag aacaaatagt acagtgattg
     6061 aagtgtctta tgagaaagac attacaccga ttacattaag tacattaaat aagcatccta
     6121 aattgaaaaa cttatatttc cttcaagaaa atgcaaaaca ggctattgca agtatgtcac
     6181 aagcacagta tgatgcaatt attgaaatga gcgataataa tggattgaaa tctccatttg
     6241 aaatggttca aaaaccagat atgcttgaaa gtgaaaaaga agaagcgtta aagccattta
     6301 ttttacttgt tgtagatcga aaagaagaag gcttaaaagc ggctaatgac ttattgcaaa
     6361 aagcgaatgc aaatcctgtt ataacaacag gtcatcctga tttttcagaa gatatgcttt
     6421 atggcaagta tttaccaaat gaaacagggg cattatacta tcgtgaaggg tttattactc
     6481 aattaatgcc taaaaaggat aaaagttatt tagttattga taattttaat cgtatagata
     6541 cagacatttt ccaaacgtat attaatgtgt tagaaggcta tgaagtaaca ttaccacgtt
     6601 ataataaaga cggtaatatg attaaatggt cacgacaaaa agattctttc tattatttca
     6661 atcctaattg gcatattgta ggtattacat atgacagttt agaggaaatt aaggagaaat
     6721 attcagagca gtttttaaaa tacacacgta tagtcaaagt caaacatgac taatgatata
     6781 tggtagtttt tgaaaagaag taaggatagg attaaaaatt gcataattgc atatttgaat
     6841 aaccgcataa agaaaaaatg acgggtaatc aaccataaga gctaattaag agcttatgat
     6901 agattactcg tttttatttt aataaataat aaagacagta tatgaatata aaagaattta
     6961 tgttacagaa ttaaaacttt atatgagttt tccagtggta tgttgaataa gacgaatgac
     7021 caaacgctaa gttaataata agaaataaga ttacactgta gattgagaaa tttaaagtaa
     7081 ataaaaaata cattatacaa taaatataaa actgaaaaat aaattttatg gcaatttcgt
     7141 gtcgcgagga cttatcaagt gacaaccttg tttaaatgtt tttaaattag gaaaaatgac
     7201 tataagtact attaaaggag cgaataaatt atgccaatcg tatattatga tggaaactgt
     7261 atatattgtt ataactatgt catttggtta attcaacatg acctaccaag aaattatcaa
     7321 tttgccacgt taaaaggtga agttggccaa caatttttta aacaacatcc agaagcggca
     7381 aataaaaata gtgtgatttt acagaaaggt gatcgtatat attttgaatc gcaggcgatt
     7441 attaaactaa tcacagcact acctaatcga actaaattat tgggcgtagg tttatggctt
     7501 gtacctaaac cagtccgtaa tttcggttat cgaatgtttg ctaataatag aaatagaatg
     7561 tggaaaacaa catggcacca accaaatgat tatgaaaaat cgttcttttt agatgataat
     7621 gcgaaagtaa aacttactga ttgataaaac a
//
LOCUS       DAMQYT010000122         7635 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMQYT010000122 DAMQYT010000000
VERSION     DAMQYT010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.122"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(5..208)
                     /locus_tag="PGA33_001914"
     CDS             complement(5..208)
                     /locus_tag="PGA33_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDG6473718.1"
                     /translation="MRKQVIITKTVVGWYNIKDTQHNLMLNIPPKVFEQYFPDVSKDV
                     QVACLEMDLSKITEIKNKKKVGS"
     gene            complement(175..348)
                     /locus_tag="PGA33_001915"
     CDS             complement(175..348)
                     /locus_tag="PGA33_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDG6473719.1"
                     /translation="MKMYLTYICLVSLLTILLLAISNMYVAFSVYGMMVTYGFNLTGE
                     ITTCENKLLLQKQ"
     gene            complement(360..632)
                     /locus_tag="PGA33_001916"
     CDS             complement(360..632)
                     /locus_tag="PGA33_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDG6473720.1"
                     /translation="MSRTKLHDVPAKENTITEPKQVVVNPLFAKPNALASILGISYSS
                     VNRILKEWEKDSKGVDDLYYSLSSTMIVISIPRFEEYMKARHKKWM"
     gene            complement(625..888)
                     /locus_tag="PGA33_001917"
     CDS             complement(625..888)
                     /locus_tag="PGA33_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDG6473721.1"
                     /translation="MVLNLKRLRAERIACGITQDEMAHKMGWKTRTPYAKRENGIVDI
                     GANEFIKMAKILGYETNNLDIFFTNNVPGKERKNILKGGELNV"
     gene            1081..1413
                     /locus_tag="PGA33_001918"
     CDS             1081..1413
                     /locus_tag="PGA33_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDG6473722.1"
                     /translation="MRTNDEIITIIKTSMKEQNMSLSELARRVGVAKSAVSRYLNLTR
                     EFPLNRAEDFAKVLGIKTEYLLGFAEREESTKQDTIAAHLDGDFTEEELIEIRKYAEL
                     VRKAHRNQ"
     gene            1425..1883
                     /locus_tag="PGA33_001919"
     CDS             1425..1883
                     /locus_tag="PGA33_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDG6473723.1"
                     /translation="MYLYEKMVIENKEIPIDDGKSLGNFEGLYDNGVILINKNLSETR
                     KAEVLYEELAHHKLTYGNILDQSKWINRKFENYARRHGFISAVPLREIVEAYNYGVRN
                     LYELSEYLQLSEKYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            1900..2628
                     /gene="seq"
                     /locus_tag="PGA33_001920"
     CDS             1900..2628
                     /gene="seq"
                     /locus_tag="PGA33_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type Q"
                     /protein_id="HDG6473724.1"
                     /translation="MNKIFRVLTVSLFFFTFLIKNNLAYADVGVINLRNFYTNYQPET
                     LQGVSSGNFSTSHQLEYIDGKYTLYSQFHNEYEAKRLKDHKVDIFGISYSGLCNTKYM
                     YGGITLANQNLDKPRNIPINLWVNGKQNTISTDEVSTQKKEVTAQEIDIKLRKYLQNE
                     YNIYGFNKTKKGQEYGYQSKFNSGFNKGKITFHLNNETSFTYDLFYTGTGQAESFLKI
                     YNDNKTIDAENFHLDVEISYEKTE"
     gene            2652..3380
                     /gene="sek"
                     /locus_tag="PGA33_001921"
     CDS             2652..3380
                     /gene="sek"
                     /locus_tag="PGA33_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type K"
                     /protein_id="HDG6473725.1"
                     /translation="MKKLIVIILINIITLSVSNSASAQGDIGIDNLRNFYTKKDFVDL
                     KDVKDNDTPIANQLQFSNVSYDLISESKDFNKFSNFKGKKLDVFGISYNGQCNNKYIY
                     GGVTATNEYLDKPRNIPINIWINGNHKTISTNKVSTNKKFVTAQEIDVKLRKYLQEEY
                     NIYGHNGTKKGEEYGHKSKFYSGFNIGKVTFHLNNNDTFSYDLFYTGDDGLPKSFLKI
                     YEDNKTVESEKFHLDVDISYKETI"
     gene            3468..4688
                     /locus_tag="PGA33_001922"
     CDS             3468..4688
                     /locus_tag="PGA33_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDG6473726.1"
                     /translation="MWLEKFKNKNKETKYRYYEKYKDPLTAKWRRVSVVLNKNGKQSQ
                     KEAQRLLNERIEEKLNDKKPTTLKSLTFHVACDEWFQNYIKTSGSKRTTIKTKLSKLN
                     TLKKFVDEDILINKITLSYAQQVFDEMDSKGYVYQVNKDALSIFKNVFEYTRRIYKLQ
                     DLEFLKDITLNKRIKSYDEVKAKRNKYLELNEIESIIKDINTKAQKMHSGIHKRFYLF
                     VALMTEFQALNGMRIGEMLAIQNEDIDFDNKSLNINGTIHWFHDESGGFGVKDTTKTE
                     SSYRTIGLSSRSCEILKKAILENKKDSKWNDGYLNRNFVFTNHKGNPMQTERFNKILR
                     EAAKDVGIDKEVSSHILRHSHISLLSQQGVSLKAIMDRVGHSDHRTTLSIYSHVTEQM
                     DKDMMNKLEQVKLG"
     gene            complement(4831..5652)
                     /locus_tag="PGA33_001923"
     CDS             complement(4831..5652)
                     /locus_tag="PGA33_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDG6473727.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            complement(5670..6365)
                     /locus_tag="PGA33_001924"
     CDS             complement(5670..6365)
                     /locus_tag="PGA33_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6473728.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            complement(6358..7383)
                     /locus_tag="PGA33_001925"
     CDS             complement(6358..7383)
                     /locus_tag="PGA33_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDG6473729.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEQGPVTQVFENPQHTVTKRFVKDDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     regulatory      complement(7472..7575)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         2670 a         1241 c         1171 g         2553 t
ORIGIN      
        1 catcttaact acctactttt ttcttatttt taatttctgt aatttttgat aaatccattt
       61 ctaaacacgc aacttgaaca tctttactaa catcaggaaa gtactgttca aatacttttg
      121 gcggtatatt taacattaaa ttatgttgag tatctttaat gttgtaccag ccaactactg
      181 tttttgtaat aataacttgt tttcgcacgt tgtaatctct cctgttaaat taaatccata
      241 agttaccatc atgccgtaca cactaaaagc gacatacatg ttagatattg ctagtaataa
      301 tattgttaac aatgaaacta agcagatata agttaagtac attttcattg ccttgcctcc
      361 tacatccatt ttttatgacg cgccttcatg tactcctcga atcgcggaat actgataaca
      421 atcattgttg atgataacga gtaatataaa tcatcaacac ctttagaatc tttttcccac
      481 tcttttaaaa tgcgattcac cgaactatat gaaattccca aaatactagc tagtgcatta
      541 ggtttcgcaa acaaaggatt cactacaact tgctttggtt ctgtaattgt attttcttta
      601 gctggtacat catgcaattt tgttctagac atttaattca cctcctttta agatgttttt
      661 tcgttctttt ccgggaacgt tattggtaaa aaaaatgtct aggttatttg tttcataacc
      721 taatattttt gccattttaa taaattcatt cgctccaata tctactattc cattttctct
      781 ctttgcataa ggcgttcttg ttttccaccc cattttgtga gccatttcat cttgcgtaat
      841 accacaagct attctttccg ctctcaatct ttttaaattt agtaccacgt ttacacctcc
      901 tatcgttctc gttcgagaac taattacaat ttaccattta cgttctcgtt cgtcaacaat
      961 ttttacttaa aaaaattcaa aaagtttttt cttccttata tattgtattc ttttgggaac
     1021 ggtgttataa tcaaatcgtt cacaaataag aacaaatatt caattcagga gatacaagaa
     1081 atgagaacta atgatgaaat aatcacaata attaaaacat caatgaaaga acaaaatatg
     1141 tcactaagtg aattagctcg tcgtgtaggt gtagcaaaat cagcagtatc aagatattta
     1201 aatttaacta gagagttccc attgaatcgt gctgaagatt ttgcgaaagt acttggaata
     1261 aaaacagaat atttattagg atttgctgaa cgcgaagaat ctacaaaaca agatactatc
     1321 gccgcgcact tagatggaga ttttacagag gaagaattaa ttgaaattag aaagtatgcg
     1381 gagttagtta gaaaagcaca tcgaaatcag taaggggtta ttgcatgtat ttatacgaaa
     1441 agatggttat tgaaaacaaa gagataccaa ttgatgatgg gaaatcttta ggtaattttg
     1501 agggtctcta tgacaatggg gttattttaa ttaataaaaa tttgtcagaa acacgtaaag
     1561 cagaagtgtt atatgaggaa cttgcccacc acaagttgac gtatggcaac attttagacc
     1621 aatcaaaatg gattaataga aaatttgaaa attatgcacg tagacacggc tttatctcag
     1681 ctgttccatt acgtgaaatt gtagaagctt ataattatgg cgtacgtaac ttgtatgagt
     1741 tgtccgagta cctacaactg agtgaaaaat acatattaga agcgatagaa caatataaaa
     1801 agatatacgg tattggtacc cactacggcg agtattcaat tacatttgag ccgttgagag
     1861 tttttaaata taaagaaata taacaaagga gaaatgaaaa tgaataaaat atttagagta
     1921 ctcactgtta gcttgttttt cttcacattt ttaataaaaa acaatctagc atatgctgat
     1981 gtaggggtaa tcaaccttag aaacttttat actaattatc aaccagaaac gcttcaagga
     2041 gttagttctg gaaatttttc tacttctcat caattagagt atattgatgg aaaatacact
     2101 ttatattcac agtttcataa tgaatatgaa gcgaagagat taaaagatca taaagtagat
     2161 atctttggaa taagttactc aggtctttgt aatacaaaat atatgtatgg cggaattacg
     2221 ttggcgaatc aaaatttaga taaacctaga aatataccta ttaatctctg ggtcaatggt
     2281 aagcaaaaca ctatatctac agacgaggtt tctactcaaa aaaaagaggt aactgctcaa
     2341 gagattgata ttaagttacg aaaataccta caaaacgaat acaatatata tggttttaat
     2401 aaaacaaaaa aaggtcagga atatggatat cagtcaaaat ttaattctgg atttaacaaa
     2461 ggaaaaatta ctttccattt aaataatgaa acttctttta catacgattt gttttacacc
     2521 ggaactggtc aagcagagag ttttttaaaa atttacaatg ataataaaac tatagatgca
     2581 gagaattttc atttggatgt agagatttca tatgagaaga ctgaataaat agaatttaag
     2641 gagaaaatgc aatgaaaaaa ttaatagtta tcatattaat aaatataata actttaagtg
     2701 tctctaatag tgccagcgct caaggtgata taggaattga taatctcagg aatttttata
     2761 caaaaaaaga ctttgtagat ttaaaagatg taaaagacaa tgatactcct atagctaatc
     2821 aactacaatt ttcaaatgta tcttatgatt taatttcaga atcaaaagat tttaataaat
     2881 ttagtaattt caagggaaaa aaacttgatg tttttggtat tagttataat ggccagtgta
     2941 acaataaata catatatggt ggagtcacag ctactaacga atatctagat aaacctagaa
     3001 atatccctat aaatatatgg atcaatggaa atcacaaaac tatttctacc aataaagttt
     3061 cgacaaataa aaaatttgtt accgctcaag agattgatgt caaattaaga aagtaccttc
     3121 aagaagaata caacatttat ggacataacg gcactaaaaa aggagaagaa tatggtcata
     3181 aatcaaaatt ttattctggg tttaatattg gtaaagtaac gttccattta aataataatg
     3241 acactttttc atatgattta ttctacacag gagatgatgg gttaccaaaa agttttttaa
     3301 aaatttacga agacaataaa actgtagagt ctgagaaatt ccatttggat gtcgatattt
     3361 cttataaaga aacgatataa atctattaat tatgtcataa tcattatttt ccgggtagca
     3421 tgcctaccct tattattttt tgccaatttt gaggagggag aagcaaaatg tggttagaaa
     3481 aatttaaaaa taagaataaa gaaactaaat atagatatta cgagaagtac aaagaccctc
     3541 tcactgccaa atggagacgt gttagcgtgg tacttaataa gaatggtaag caatcacaaa
     3601 aagaagctca aagactctta aatgagcgta tagaagaaaa actgaatgat aagaaaccta
     3661 ctacacttaa gtcactaact ttccatgtcg catgtgatga gtggtttcag aattatatca
     3721 aaacgtctgg gtcaaaaaga acaactatta aaactaaatt gagtaagcta aacactttga
     3781 agaaatttgt agatgaagat attttaatca ataaaataac actttcatat gcgcaacaag
     3841 ttttcgatga aatggatagt aaaggttatg tatatcaagt aaacaaagat gcgttaagca
     3901 tattcaaaaa tgtatttgaa tacactagac gtatctataa actgcaagat ttagaatttt
     3961 taaaagatat aacattaaat aaaagaatta agtcttatga tgaagtgaaa gctaaacgta
     4021 ataagtacct cgaattaaat gaaatagaat ctatcatcaa agatattaat acgaaggctc
     4081 agaagatgca ctcaggtatc cataagcggt tttatttatt cgttgcactc atgacagaat
     4141 tccaagcctt aaatggtatg cgtattggtg aaatgttagc cattcaaaat gaagatatag
     4201 actttgataa taagagttta aatattaacg gaacaatcca ttggtttcac gatgaatctg
     4261 gtggattcgg tgtaaaagat accactaaaa cagagtctag ttatagaaca attggattga
     4321 gtagcagaag ttgcgagata ttaaagaaag caatactgga gaacaaaaag gattcaaaat
     4381 ggaatgatgg atatctaaat agaaattttg tatttactaa tcataaaggc aacccaatgc
     4441 agactgaaag attcaataaa atccttaggg aagcagctaa agatgttggt attgataaag
     4501 aagtttcttc acatatatta agacatagcc acatatcatt actctctcaa caaggcgtgt
     4561 cacttaaagc tattatggat cgtgtaggcc attccgacca ccgtacaaca ctttccattt
     4621 attctcacgt tactgaacag atggataagg atatgatgaa caaacttgaa caggtaaaac
     4681 ttggataatg cgctcaaatc tgagcgtatt tttttgttta aaattcgata atttgcatat
     4741 actgcccctt ttgtgccctt ttttattttc aataccacaa aaataacccc ttaatcgttg
     4801 ttattaaagg gtatttttat ttaattggta ttatttagca ggaataactg caccattgta
     4861 tttttcatta atgaagtctt gaatatcttt agattgtaat acttcaatta atgctttgat
     4921 tttcttatca tcttgatgtc cttctttaac agcaattaag tttgcataag gattatcttt
     4981 cgcactttct acagcaatag aatctttttt aggatttagt ttttgttcga ttgcaaagtt
     5041 cgaattaatg ataacagcat cagcgtcttc attttgataa attttaggta agaattctgc
     5101 tgattgttta ttattaaact taatatcttt tttattctct gtaatatcac taaacttagc
     5161 atcttcaatt tttacgcctt ttttgatttt aattaaacct gcatcaacga agaattttaa
     5221 gaaacgtcct tgttcagctg gattattaga cacatagact gttgcacctt ttggtaattc
     5281 ttttaaactt ttatactttt tagagtatac agccataggt tctaagtgaa catcaccggc
     5341 acttacgatt ttgtaacctt tatccttttt ctctgtgttt aaatatggtg tatgttggaa
     5401 atagtttgcg tcaatttcac ctttgtctag taatttatta ggtgtagtgt aatcgttaat
     5461 tgttttaata tctagttcat aacctttttt ctctaataat ggttttgctt tttctaaaat
     5521 ttcagcatgt ggtgctggtg aagcacctac tgttattttc ttgtcgtcac tgccactttt
     5581 gtttccatta ccgcaagctg ctaatacaac tgcaaatgtt aatactaaaa taagaccaaa
     5641 taattttttc ataaaatgaa acccccaatt tatcgtttat caagtttatt tgtaagccaa
     5701 tccccaatga attggattat aaatacaata attaaaataa aaactgttga tactaaaatg
     5761 acatcatttt gatttcgagt gaaacctgtt aagtatgcta aatttcctaa accaccggca
     5821 ccaattacac ctgcaactgc tgttgaacca actaaagcga ttgctgtaac tgtaatgcca
     5881 gacactagcg ctggcatagc ttcaggtaaa aggactttac gaattactgt ccaagtatta
     5941 gcgcccattg accaagccgc ttcgatgaca cctttatcaa tttctttaaa agcaatttct
     6001 acgagccttg cataaaacgg tgctgcgcca atgatcaagg ctggtaacgc acctgtcgga
     6061 ccacttatcg ttccaagtat caaacttgta aatggaatta ataataaaat taaaataata
     6121 aatggtatcg ctctaaataa gttaacaatg aaagaaacga tagaataaaa taaccttgca
     6181 ccgatagact tacctttagc agacaagaat aataacacac ctaaaataag accaagtata
     6241 aatgcaaata tagttgagac gactgtcatg tatagtgttt cgactattgc agtccaaact
     6301 tctggccact gaatattagg cattgtaatc atttcattta taatttcact aaatgattta
     6361 cccatgtctt aacacctcca ttttaacttg tcgctcaatt aactcttttt cgaattttcc
     6421 gaaatctaca cttgaaatat atggaatatg cagaactaaa aagccgactg ttccattttt
     6481 tgtattttta atatttgctt ctaaaatatt aattttaata tcataggcag ttgatagact
     6541 cgatacaata ggctcggttg ttgttgaacc agcgaaaact aatctaacga tatatgcatc
     6601 tttttctaat ggctctaatt ctgttaaaga tgtttcgaaa tcatcattta aatcgtcttt
     6661 cacaaatcgt tttgtcacag tgtgttgcgg attttcaaaa acctgtgtca ccggtccttg
     6721 ttctatcact ttaccacttt ccataactgc aacttcatca caaatacgac gaatgacatg
     6781 catttcatgc gtaattagta caattgttaa attttgttgt tctctaattt ttagtagtag
     6841 atctaaaatt tcatctgttg tttgcggatc aagtgcactt gttgcctcat cacaaagcaa
     6901 gaccgttgga tcattagcta acgctcgtgc aatcccaaca cgttgctttt gtccacctga
     6961 taactctgat ggataagcct tttctctacc ttttaaaccg acgagttcga caagttctaa
     7021 tgctttttgc ttagctctcc ttctagggac acctgcaatt tcaagcggaa acataatatt
     7081 ttttaacaca gtccttgacc ataacaaatt aaaatgttgg aagatcatac ttactttttg
     7141 tctttttgct cttaatccat ttttggacaa ttgacctata tggtctccat ctataataac
     7201 ttcacctgat gtaggcgctt ctaaatgatt aaacattcga atcaaagtac tttttcctgc
     7261 tccagaaaaa ccaatgacgc cataaatcga tcctgctcga atcgataaat taacgtgatc
     7321 tacagcaagg acttctttat ttttagtccg atattcttta acaacttctt ttaattcaat
     7381 cacgttgatt cctccctgtg ttgcttaata aaataaaaaa tgctttctca atatcgatag
     7441 aaaaattgag aaagcaatag tagtattgtt tctctcatct tcaaaagtta aaactttatg
     7501 tgaattggca ccatttctat ataagacggt tgccgggctt cgtagggcac atccctccac
     7561 cactctcgat aagagtttac gcatcattta atttgtatta atcctaacac cttagtaaaa
     7621 tttcgtcaat aacta
//
LOCUS       DAMQYT010000123         7494 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMQYT010000123 DAMQYT010000000
VERSION     DAMQYT010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.123"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..2828
                     /gene="sdrC"
                     /locus_tag="PGA33_001926"
     CDS             <1..2828
                     /gene="sdrC"
                     /locus_tag="PGA33_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrC"
                     /protein_id="HDG6473730.1"
                     /translation="ATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLSGHEAKA
                     AEHTNGELNQSKNETTAPSENKTTKKVDSRQLKDNTQTATADQPKVTMSDSATVKETS
                     SNMQSPQNATANQSTTKTSNVTTNDKSSTTYSNETDKSNLTQAKDVSTTPKTTTIKPR
                     TLNRMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHVNQTTGKTEFWATSSDVLKLKA
                     NYTIDDSVKEGDIFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDSTTNTTTY
                     TFTNYVDQYTNVRGSFEQVAFAKRKNATTDKTAYKMEVTLGNDTYSEEIIVDYGNKKA
                     QPLISSTNYINNEDLSRNMTAYVNQPKNTYTKQTFVTNLTGYKFNPNAKNFKIYEVTD
                     QNQFVDSFTPDTSKLKDVTDQFDVIYSNDNKTATVDLMKGQTSSNKQYIIQQVAYPDN
                     SSTDNGKIDYTLDTDKTKYSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTNKDGKQD
                     ANEKGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGYTPTTAN
                     VGTDDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKG
                     IKGVKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGTNTTEDD
                     KDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDNDSDSDSDSDSDAGKHTPAKPMSTVKDQHKTAKALPETGSENNNS
                     NNGTLFGGLFAALGSLLLFGRRKKQNK"
     gene            3195..7340
                     /gene="sdrD"
                     /locus_tag="PGA33_001927"
     CDS             3195..7340
                     /gene="sdrD"
                     /locus_tag="PGA33_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrD"
                     /protein_id="HDG6473731.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKLIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQPDL
                     QENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNDETLVDNNSNSNNENNADII
                     LPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLS
                     LKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVQMGINYSIYMDADTIPVSKNDVEFNVTIGNTTTKTTANIQ
                     YPDYVVNEKNSIGSAFTETVSHVGNKENPGYYKQTIYVNPSENSLTNAKLKVQAYHSS
                     YPNNIGQINKDVTDIKIYQVPKGYTLNKGYDVNTKELTDVTNQYLQKITYGDNNSAVI
                     DFGNADSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKSVSTGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGN
                     YKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKDVKVTLLNEKGEVIGTTKTDENGKYCFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDRDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDRDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DAGKHTPVKPMSTTKDHHNKAKALPETGNENSGSNNATLFGGLFAALGSLLLFGRRKK
                     QNK"
BASE COUNT         3021 a         1180 c         1336 g         1957 t
ORIGIN      
        1 cagcaacaaa tagaaaaggc atgataccaa atcgattaaa caaattttcg ataagaaagt
       61 attctgtagg tactgcttca attttagtag ggacaacatt gatttttggg ttaagtggtc
      121 atgaagctaa agcggcagaa catacgaatg gagaattaaa tcaatcaaaa aatgaaacga
      181 cagccccaag tgagaataaa acaactaaaa aagttgatag tcgtcaacta aaagacaata
      241 cgcaaactgc aactgcagat cagcctaaag tgacaatgag tgatagtgca acagttaaag
      301 aaactagtag taacatgcaa tcaccacaaa acgctacagc taatcaatct actacaaaaa
      361 ctagcaatgt aacaacaaat gataaatcat caactacata tagtaatgaa actgataaaa
      421 gtaatttaac acaagcaaaa gatgtttcaa ctacacctaa aacaacgact attaaaccaa
      481 gaactttaaa tcgcatggca gtgaatactg ttgcagctcc acaacaagga acaaatgtta
      541 atgataaagt acatttttca aatattgaca ttgcgattga taaaggacat gttaatcaga
      601 ctactggtaa aactgaattt tgggcaactt caagtgatgt tttaaaatta aaagcaaatt
      661 acacaatcga tgattctgtt aaagagggcg atatatttac ttttaaatat ggtcaatatt
      721 tccgtccagg atcagtaaga ttaccttcac aaactcaaaa tttatataat gcccaaggta
      781 atattattgc aaaaggtatt tatgatagta caacaaacac aacaacatat acttttacga
      841 actatgtaga tcaatataca aatgttagag gtagctttga acaagttgca tttgcgaaac
      901 gtaaaaatgc aacaactgat aaaacagctt ataaaatgga agtaacttta ggtaatgata
      961 catatagcga agaaatcatt gtcgattatg gtaataaaaa agcacaaccg cttatttcaa
     1021 gtacaaacta tattaacaat gaagatttat cgcgtaatat gactgcatat gtaaatcaac
     1081 ctaaaaatac atatactaaa caaacgtttg ttactaattt aactggatat aaatttaatc
     1141 caaatgcaaa aaacttcaaa atttacgaag tgacagatca aaatcaattt gtggatagtt
     1201 tcacccctga tacttcaaaa cttaaagatg ttactgatca attcgatgtt atttatagta
     1261 atgataataa aacagctaca gtcgatttaa tgaaaggcca aacaagcagc aataaacaat
     1321 acatcattca acaagttgct tatccagata atagttcaac agataatgga aaaattgatt
     1381 atactttaga cactgacaaa actaaatata gttggtcaaa tagttattca aatgtgaatg
     1441 gctcatcaac tgctaatggc gaccaaaaga aatataatct aggtgactat gtatgggaag
     1501 atacaaataa agatggtaaa caagatgcca atgaaaaagg gattaaaggt gtttatgtca
     1561 ttcttaaaga tagtaacggt aaagaattag atcgtacgac aacagatgaa aatggtaaat
     1621 atcagttcac tggtttaagc aatggaactt atagtgtaga gttttcaaca ccagccggtt
     1681 atacaccgac aactgcaaat gtaggtacag atgatgctgt agattctgat ggactaacta
     1741 caacaggtgt cattaaagac gctgacaaca tgacattaga tagtggattc tacaaaacac
     1801 caaaatatag tttaggtgat tatgtttggt acgacagtaa taaagatggt aaacaagatt
     1861 cgactgaaaa aggaattaaa ggtgttaaag ttactttgca aaacgaaaaa ggcgaagtaa
     1921 ttggtacaac tgaaacagat gaaaatggta aataccgctt tgataattta gatagtggta
     1981 aatacaaagt tatctttgaa aaacctgctg gcttaactca aacaggtaca aatacaactg
     2041 aagatgataa agatgccgat ggtggcgaag ttgatgtaac aattacggat catgatgatt
     2101 tcacacttga taatggctac tacgaagaag aaacatcaga tagcgactca gattctgaca
     2161 gcgattcaga ctcagatagc gactcagatt cagatagcga ctcagattca gacagcgatt
     2221 cagacagcga ctcagactca gatagcgatt cagattcaga cagcgactca gactcagaca
     2281 gcgattcaga ctcggatagc gactcagact cagatagcga ctcagattcg gatagcgact
     2341 cagactcaga tagcgattca gattcagata gcgattcgga ctcagacagt gattcagatt
     2401 cagactcaga tagcgactca gattctgaca gcgattcaga ctcagacagc gactcagact
     2461 cagacagtga ttcagattca gacagcgact cagattcaga tagcgactca gactcagata
     2521 gcgactcaga ctcagatagc gactcagact cggatagcga ttcagattca gacagcgact
     2581 cagattcaga tagcgattcg gactcagaca acgactcaga ttcagatagc gattcagatt
     2641 cagatgcagg taaacatact ccggctaaac caatgagtac ggttaaagat cagcataaaa
     2701 cagctaaagc attaccagaa acaggtagtg aaaataataa ttcaaataat ggcacattat
     2761 tcggtggatt attcgcggca ttaggatcat tattgttatt cggtcgtcgt aaaaaacaaa
     2821 ataaataata tgattaactt aaccaggtcc atgtggcctg gttttttctg tttagaaatt
     2881 cccagatata aaacaaaaaa tctttaagga atagatacaa tttaatttgt tacagaaatg
     2941 taattgtcta tgaattagaa aagctaagat attaatttca agggcaaata atactatttt
     3001 ttatgatgat taaataaaga ttacaaaact cataaaaact tttatttaaa cacaaatgtt
     3061 aaataacgtt ttacgataaa gaaaaataat taaagtattg tgctttatct aaaaatgtaa
     3121 tactatattg atatacattt ttgtatttaa aacaattgta ttatttaaaa atttgatgaa
     3181 ttaggagtaa tctaatgcta aacagagaaa ataaaacggc aataacaagg aaaggcatgg
     3241 tatccaatcg attaaataaa ttttcgatta gaaagtacac agtgggaaca gcatcaattt
     3301 tagtaggtac aacattaatt tttggtctgg ggaaccaaga agcaaaggct gcagaaagta
     3361 ctaataaaga attgaacgaa gcgacaactt cagcaagtga taatcaatcg agtgataaag
     3421 ttgatatgca gcaactaaat caagaagaca atactaaaaa tgataatcaa aaagaaatgg
     3481 tatcatctca aggtaatgaa acgacttcaa atgggaataa attaatagaa aaagaaagtg
     3541 tacaatctac cactggaaat aaagttgaag tttcaactgc caaatcagat gagcaagctt
     3601 caccaaaatc tacgaatgaa gatttaaaca ctaaacaaac tataagtaat caagaagcgt
     3661 tacaacctga tttgcaagag aataaatcag tggtaaatgt tcaaccaact aatgaggaaa
     3721 acaaaaaggt agatgccaaa actgaatcaa ctacattaaa tgttaaaagt gatgctatca
     3781 agagtaatga tgaaactctt gttgataaca atagtaattc aaataatgaa aataatgcag
     3841 atatcatttt gccaaaaagt acagcaccta aacgtttgaa tacaagaatg cgtatagcag
     3901 cagtacagcc atcatcaaca gaggctaaaa atgttaatga tttaatcaca tcaaatacaa
     3961 cattaactgt cgttgatgca gataaaaaca ataaaatcgt accagcccaa gattatttat
     4021 cattaaaatc acaaattaca gttgatgaca aagttaaatc aggtgattat ttcacaatta
     4081 aatactcaga tacagtacaa gtatatggat tgaatccgga agatattaaa aatattggtg
     4141 atattaaaga tccaaataat ggtgaaacaa ttgcgactgc aaaacatgat actgcaaata
     4201 atttaattac atatacattt acagattatg ttgatcgatt taattctgta caaatgggaa
     4261 ttaattattc aatttatatg gatgctgata caattcctgt tagtaaaaac gatgttgagt
     4321 ttaatgttac gataggtaat actacaacaa aaacaactgc taacattcaa tatccagatt
     4381 atgttgtaaa tgagaaaaat tcaattggat cagcgttcac tgaaacagtt tcacatgttg
     4441 gaaataaaga aaatccaggg tactataaac aaacgattta tgtaaatcca tcggaaaatt
     4501 ctttaacaaa tgccaaacta aaagttcaag cttaccactc aagttatcct aataatatcg
     4561 ggcaaataaa taaagatgta acagatataa aaatatatca agttcctaaa ggttatacat
     4621 taaataaagg atacgatgtg aatactaaag agcttacaga tgtaacaaat caatacttgc
     4681 agaaaattac atatggcgac aacaatagcg ctgttattga ttttggaaat gcagattctg
     4741 cttatgttgt aatggttaat acaaaattcc aatatacaaa tagcgaaagc ccaacacttg
     4801 ttcaaatggc tactttatct tcaacaggta ataaatccgt ttctactggc aatgctttag
     4861 gatttactaa taaccaaagt ggcggagctg gtcaagaagt atataaaatt ggtaactacg
     4921 tatgggaaga tactaataaa aacggtgttc aagaattagg agaaaaaggc gttggcaatg
     4981 taactgtaac tgtatttgat aataatacaa atacaaaagt aggagaagca gttactaaag
     5041 aagatgggtc atacttgatt ccaaacttac ctaatggaga ttaccgtgta gaattttcaa
     5101 acttaccaaa aggttatgaa gtaacccctt caaaacaagg taataacgaa gaattagatt
     5161 caaacggctt atcttcagtt attacagtta atggcaaaga taacttatct gcagacttag
     5221 gtatttacaa acctaaatac aacttaggtg actatgtctg ggaagataca aataaaaatg
     5281 gtatccaaga ccaagatgaa aaaggtatat ctggcgtaac ggtaacatta aaagatgaaa
     5341 acggtaacgt gttaaaaaca gttacaacag acgctgatgg caaatataaa tttactgatt
     5401 tagataatgg taattataaa gttgaattta ctacaccaga aggctataca ccgactacag
     5461 taacatctgg tagcgacatt gaaaaagact ctaatggttt aacaacaaca ggtgttatta
     5521 atggtgctga taacatgaca ttagatagtg gattctacaa aacaccaaaa tataatttag
     5581 gtaattatgt atgggaagat acaaataaag atggtaagca ggattcaact gaaaaaggta
     5641 tttcaggcgt aacagttaca ttgaaaaatg aaaacggtga agttttacaa acaactaaaa
     5701 cagataaaga tggtaaatat caatttactg gattagaaaa tggaacttat aaagttgaat
     5761 tcgaaacacc atcaggttac acaccaacac aagtaggttc aggaactgat gaaggtatag
     5821 attcaaatgg tacatcaaca acaggtgtca ttaaagataa agataacgat actattgact
     5881 ctggtttcta caaaccgact tacaacttag gtgactatgt atgggaagat acaaataaaa
     5941 acggtgttca agataaagat gaaaagggca tttcaggtgt aacagttacg ttaaaagatg
     6001 aaaacgacaa agttttaaaa acagttacaa cagatgaaaa tggtaaatat caattcactg
     6061 atttaaacaa tggaacttat aaagttgaat tcgagacacc atcaggttat acaccaactt
     6121 cagtaacttc tggaaatgat actgaaaaag attctaatgg tttaacaaca acaggtgtca
     6181 ttaaagatgc agataacatg acattagaca gtggtttcta taaaacacca aaatatagtt
     6241 taggtgatta tgtttggtac gacagtaata aagacggcaa acaagattca actgaaaaag
     6301 gtatcaaaga tgttaaagtt actttattaa atgaaaaagg cgaagtaatt ggaacaacta
     6361 aaacagatga aaatggtaaa tactgctttg ataatttaga tagcggtaaa tacaaagtta
     6421 tttttgaaaa gcctgctggc ttaacacaaa caggtacaaa tacaactgaa gatgataaag
     6481 atgcagatgg tggcgaagtt gacgtaacaa ttacggatca tgatgatttc acacttgata
     6541 atggatacta cgaagaagaa acatcagata gcgactcaga ttcggacagc gactcagatt
     6601 cagacagaga ctcagactca gatagtgatt cagactcgga tagcgattca gattcagaca
     6661 gcgattcaga ttcagatagc gattcagatt cagacagaga ctcagatagt gattcagact
     6721 cagatagcga ctcagattca gacagcgact cagattcaga cagcgactca gactcagata
     6781 gtgattcaga ctcagatagc gactcagatt cagacagcga ctcagactca gacagcgact
     6841 cagactcaga tagtgactca gattcagata gcgactcaga ttcggacagc gattcagact
     6901 cagatagcga ctcagattca gatagcgatt cggactcaga tagcgactca gattcagata
     6961 gtgattcaga ctcagatagc gactcagatt cagacagcga ctcagattcg gatagcgact
     7021 cagattcaga cagcgactca gactcggata gtgattcaga ctcagatagc gactcagact
     7081 cagatagcga ttcagattca gatagcgact cagactcaga cagcgattca gactcagaca
     7141 gcgactcaga ctcagatgca ggtaagcaca cacctgttaa accaatgagt actactaaag
     7201 accatcacaa taaagcaaaa gcattaccag aaacaggtaa tgaaaatagc ggctcaaata
     7261 acgcaacgtt atttggcgga ttattcgcag cattaggatc attattgtta ttcggtcgtc
     7321 gtaaaaaaca aaataaataa tacagcttat accaggtccg tgaggcctgg ttttttattt
     7381 caaaatttat gaaatgtatc ttatgtttga gattaaatag tgcaattttt taaaaaatat
     7441 aacataaatc aacataaatt taattcatct ttacattaaa agtacaaacg aata
//
LOCUS       DAMQYT010000124         7494 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMQYT010000124 DAMQYT010000000
VERSION     DAMQYT010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.124"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            64..1827
                     /locus_tag="PGA33_001928"
     CDS             64..1827
                     /locus_tag="PGA33_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6473732.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNTYTINSIMVYIFIALIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATVYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
     gene            1852..3585
                     /locus_tag="PGA33_001929"
     CDS             1852..3585
                     /locus_tag="PGA33_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6473733.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            3816..3983
                     /locus_tag="PGA33_001930"
     CDS             3816..3983
                     /locus_tag="PGA33_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473734.1"
                     /translation="MQLLILTDIFYRIAVYICPNSLRSLSHFNSKVKILPKFIVILRS
                     LNSCFLEKHAP"
     gene            4037..4723
                     /gene="bioD"
                     /locus_tag="PGA33_001931"
     CDS             4037..4723
                     /gene="bioD"
                     /locus_tag="PGA33_001931"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDG6473735.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTDSYIEKDNQMTIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            4701..6059
                     /gene="bioA"
                     /locus_tag="PGA33_001932"
     CDS             4701..6059
                     /gene="bioA"
                     /locus_tag="PGA33_001932"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDG6473736.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSKKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            6061..7068
                     /gene="bioB"
                     /locus_tag="PGA33_001933"
     CDS             6061..7068
                     /gene="bioB"
                     /locus_tag="PGA33_001933"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDG6473737.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNEVSRA
                     N"
     gene            7046..>7494
                     /locus_tag="PGA33_001934"
     CDS             7046..>7494
                     /locus_tag="PGA33_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HDG6473738.1"
                     /translation="MKFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHL"
BASE COUNT         2771 a          959 c         1401 g         2363 t
ORIGIN      
        1 cagtgaaata aataatataa gcggcttaat ttagattatt aattgaaagg agttacatat
       61 acgatgaaca atcagacaaa ttggataaag atactcagcg gatttgcttc tgatagtaaa
      121 tggaaaatta tgttatcaat tttgttgtct atcatcagtg tcttttctgg attagttcct
      181 tattgggcag tatttaaaat tattttaatg atgattaaca atacatatac gattaattcg
      241 attatggttt atatctttat tgctttaatc gcttatattt cacaagtgtg ttgttttgga
      301 gcgtcaacga tgctatcaca tattacggca tatgaaattt tatctgaaat tcgtaaaaaa
      361 ttagctcaaa aattaatgcg tctcccttta ggcgtagtgg aatctaagaa aataggtgaa
      421 ttaaaaaata tatttgtcga taaggttgaa acaatagaat tacctttagc acatatgatt
      481 cctgaagtta taggaaactt acttttgtca gctgctatct ttttatacat aatgctcatt
      541 gattggcgta tggctagtgc cttattagta acgataccaa tttcaatttt cgcttttaaa
      601 aaagttatgt ctggatttaa tgagacatat gctgaacaaa tgaaatcgaa taattatatg
      661 aatagcgcga ttgtagagtt tatcgaaggc attgaagtga ttaaaacatt taatcaatct
      721 caaagttcat ataaaaaata taaagatgca gtagataatt ataagattca cactttgaat
      781 tggtttaaaa atacatgggg gtatatgaat ttaggagcta gtgtattacc ttcaacattt
      841 ttagggattt taccggtcgg catgtatttg atatctatca accaattaaa ctatgcggaa
      901 tttttcctat gcatcgtatt atctttaggt gtagtagcac cgattaagaa ttttactaat
      961 tatgtaaatc atttaaagtc tatacaatac gcattaactg aagtgaatca aatattaagt
     1021 ctagaagagt tagtattgtc aacaaaattt aagaagcctc aacattatga aattgctttt
     1081 aataatgttg gattttcata taacaaagat aaggacgatc tggtctttaa gcatttatca
     1141 tttacagtac cagaaaataa tttcacagct atcgtcgggg catctggtag cggtaagtca
     1201 accattgcca agcttatatc acgatactgg gatgtgactt caggtgaaat tacgattggc
     1261 ggtataaata taaaagatat tgaatcgaaa caacttaacg atttagttgg ttttgttgga
     1321 caagataact ttttattaaa tcttactttt aaagaaaata ttaaacttgg caatccagaa
     1381 gctacggatg aggcagttga aaaagctgcc aagttagcac aatgtcatga atttattgaa
     1441 aagttgccag atggatatga tacgaatgtt ggtacagtgg gagataaatt gtctggtggt
     1501 gaaaaacaac gagtcactat tgcaagaatg atattaaaag atgcgcctat cattgtatta
     1561 gatgaagcga ctgtttacgt tgatccagac aacgaacaaa aaattcaaga agcattaaat
     1621 gtgttgacgc aggataaaac attgattgtt attgcacatc ggttatctac gattcaacat
     1681 gcagatcaaa ttattgtttt aggtaaacaa caaattttag aaaaagggtc acatcactta
     1741 ttgctaaaat taaacggtaa ttataagaaa atgtgggata cgcacatgca tacaaaagat
     1801 tggggaatca atactggaca taattaaact aatgagaaaa ggaggtcaaa tatgtttcaa
     1861 attacattta aaattttaaa ctggataaga ccatataaag caagaatgat tttaggattt
     1921 agcatgtcat ttttaaatgc tatttttatt gcgttaccta tctttttagc tgcaaaaata
     1981 tttaataatg tgctatctca taaacctatt tatatgaaag atatattgaa tgttgtaatt
     2041 atcatggttt tattagtgat tggacgattt attacagcat atttcaaaag caaaagccat
     2101 gagagcatcg cttatgaaat gagtgcgaaa gaacgtttag atatagggga taaattgaaa
     2161 aatgtaaggt taggttattt taattcgcat cattcaaatg agttaacaac aatagtaaca
     2221 acagatttaa cctttttaga aaactttgct atgaaaatgg tggacgttgt tgttaatgga
     2281 tacatattaa ttacagtact catattgtct ctacttgtag tttcgtggca agtatcatta
     2341 ttagcatgca ttggcgtatt actatcattt tttgcgattc aattattaga aagaaagagt
     2401 cgacaaaatg cgccagcgta tcataatgta caaaaccaat tagtggaaaa ggtattggag
     2461 gttattcgtg gtattcaagt aataaaatca ttcgcgaaag aaaatacgag tcttaaaagt
     2521 tttaaccaag cagtcaatga aagtaaacgt ataaatacaa aaatagaaat gcaatatatc
     2581 ccattcaatt tattgcattt acttagttta aaagttgttt caataatgat tgtattagtt
     2641 gcatgcttat tatatatgaa tcatagtatt gatttaccta cccttattat gatttcaatt
     2701 ttttcatttg tgatatttga tagtgttgaa aatattaata gtgctgcaca cgtacttgaa
     2761 atgatagata tgacgataga tgatattgaa aagataaaaa atgctccaga actggatgag
     2821 aatggaaaaa atttgacgat taaaaatgaa aatatcgctt ttcaaaacgt gaacttttca
     2881 tatgatgata aacaagtgat aaagaatgtg aattttgaga tacctacaca aacatcaaca
     2941 gcaataattg gaccttcagg aagtgggaaa tctacattat gtcacttact cttgcgcttt
     3001 tatgatatcg atgatggaaa tattcgcatc gatggtgttg atattaaaga tatgacatta
     3061 agtacgttaa tgtcgaaaat tagtgcagta tttcaaaagg tgtatttatt taatgatacg
     3121 attgaaaata acatattgtt tggcaatcca ggtgcaacga aagaagaaat tattcgtgca
     3181 gcgaagcaag catgttgcca cgacttcatc atgtcattac ctgagggata tcaaacaatg
     3241 ctaaatgaaa aaggtagtaa tttatctggc ggagaaaagc aaaggatttc tattgctagg
     3301 gcgatattaa aagatgcacc aataattatt ttagatgaag caactgcaag tattgaccct
     3361 gaaaatgaac agctgattca aacggcaatt aatgaattaa gtaaaggcaa aacagtaatt
     3421 acaattgcac ataaacttga aactattaaa aatgcagatc agattatagt gctcaatgaa
     3481 ggtgaaataa ttcaaaaagg tagtcatgat gaattaattc gaaaaccagg aatgtatcaa
     3541 gactttataa gaataaaaag taaatcagca ggctggaaat tataaataat agtttaatat
     3601 ggaatcagaa tttggaaata aaaagggtga tagataatca acagcaaaat aaatcccctc
     3661 actagggcaa tagtgagggg atttggtgta tttagatgtt caatttatta tgtaactaat
     3721 tatagcattg atgaacaagg cgctcaacgt aatattacaa ttaatggatt gtttcacaag
     3781 atggtaaagc taggatgtct ttttattaag aaataatgca attattaatt ttaactgaca
     3841 tattttatcg aatcgctgtt tatatctgcc cgaattcctt aagaagtctt agtcatttca
     3901 atagcaaggt caaaattttg ccaaaattta tagttatact gagaagttta aattcatgtt
     3961 ttcttgaaaa acatgcgcct taaatgtaaa cttattaatt ataaaagttt acattttgat
     4021 tgaggtggtt atttttatga ggatttttat tacaagtacg aatactgatg taggcaaaac
     4081 ctatgttaca aagcatttat accatgcttt gaaaacacgt ggtcatcgag tttgtatttt
     4141 taaaccattt caaactgagg aacgccaaga cgggacgttt ccagatttag aagtatttaa
     4201 aaatgaatgt gatttaagct atgacataac gtcactttat acttttaagc aacctgtatc
     4261 accacacctt gcatttaaaa tgacagatca aatttttcta aataagcagc gtgtattaga
     4321 taaggtaaaa gttttagata aggaatttga ttttatctta attgagggtg ctgggggaat
     4381 tgccgtacca atatatgaag gtacagatga tttctacatg actaaagatc taatcaatga
     4441 ttgtgcagat tgtgtcatca gtgtgttgcc atcaaaatta ggtgctatta gcgatgccat
     4501 tgttcaccaa gattatgtta atcagaatgt atcggcgagt aattttttaa taatgaatcg
     4561 ctatacagac agctatattg aaaaagacaa tcaaatgacg attggaaaat taacaaataa
     4621 aacagtctat acatttgaag aacatgccac gtatgaaaat ttctcagaag catttttaaa
     4681 acaattaata ggagttaaaa atgaattaca cacaacaact taaacaaaaa gactcagaat
     4741 atgtttggca tccatttaca caaatgggtg tatatagcaa agaagaagca atcatcattg
     4801 aaaaaggaaa gggtagttac ctttacgata cgaatggcaa taaatattta gatggttatg
     4861 catcgttgtg ggtcaatgtg catggtcata ataacaaata cttaaataag gtaattaaaa
     4921 agcaactcaa taaaattgcc cattctacgc tgctaggatc atcaaatatt ccgtcaatag
     4981 aacttgcgga aaaattaatc gaaatcacgc caagtaatct aagaaaagta ttttattctg
     5041 atacaggcag tgcgtctgtt gaaatcgcaa taaagatggc atatcagtat tggaaaaata
     5101 ttgatagaga aaaatatgcc aagaaaaaca agtttataac gctaaatcac ggttatcatg
     5161 gggatacgat tggtgcggta agtgttggtg gtatcaagac ctttcataaa atatttaaag
     5221 acttaatatt tgagaatatt caagtagaaa gcccatcttt ctatcgcagt aattacgata
     5281 ctgaaaatga aatgatgaca gctattttaa cgaatataga gcaaattcta attgaaagaa
     5341 atgatgaaat cgcagggttt atattggaac cgttgattca aggtgcgaca ggcttgtttg
     5401 ttcatcctaa aggctttttg aaagaagtcg agaaattgtg caaaaaatac gatgtcttat
     5461 taatttgtga tgaggtagca gttggttttg ggagaactgg aaagatgttt gcatgcaatc
     5521 atgaagatgt tcaaccggat attatgtgtt taggtaaggc gattactggt ggctacttac
     5581 cacttgcagc tacattgaca tctaaaaaaa tatacaatgc atttttaagt gattcgcatg
     5641 gtgtgaatac ctttttccat ggtcatacat acaccggaaa tcaaatcgtt tgtacggttg
     5701 cattagaaaa tataagactt tatgaaaaac gtaagttatt gtcacatatt gaaacgacat
     5761 catcaacact tgagaaacag ttacatgcgc tgaagcgtca tcgaaatgtt ggtgatgtaa
     5821 gaggacgagg cttaatgttt ggagttgaat tagttacaga taaagatagt aaaacgccgt
     5881 tagaaattga aaaagttgaa cgtattgtac gtaattgtaa agaaaacggg ctaatgatta
     5941 gaaatttaga aaatgtcatt acgtttgtgc cagtgttaag tatgtcaaat aaagaagtga
     6001 aaacgatggt acgtattttt aaaaaggcag tacataacat tttagatagg aagtgttaat
     6061 atgaatttgg ctaaacgcat attacaaggg gaacaattaa caaaagagac tgtattgaaa
     6121 atttatgagg atactaatat tgatacctta gatttattaa atgaggcgta cattttaaga
     6181 aaacattatt ttggtaaaaa agtaaaatta aacatgattt taaatgctaa aagtggtata
     6241 tgtcctgaaa attgtgggta ctgtggacaa tcacgagata ttaaacaaaa acagcgatat
     6301 gctttaattc cagaggaaca aattatcgat ggagcaaagg tggcacatga taatcatatt
     6361 ggaacatatt gtattgttat gagtggtaga ggaccgagcg ataaagaagt tgatcatatt
     6421 agtaatactg taagaacgat taaatctcaa cacccgcaac taaaaatctg tgcatgttta
     6481 ggattaacga atgacgaaca agctaagaaa cttaagtcag ctggtgtaga cagatataac
     6541 cacaatatta atacaagtga aaattaccat gataacgtcg tgacaacgca tagttataaa
     6601 gatagaacag atacgataga actaatgaaa gcgaataata tatcaccatg ttctggcgtg
     6661 atttgtggta tgggagaatc taatcaagat attgttgata tggcatttgc tttaaaagaa
     6721 atggatgccg acagtattcc gattaatttt ttgcatccaa tcaaaggcac aaagtttgga
     6781 agcatggatg atttaacacc aatgaaatgt ttaagaatcg tagcattatt ccgattaatc
     6841 aatcctacga aagaaattcg tattgctgga ggaagagagg tcaacttacg ttcgttacag
     6901 ccattagcat taaaagcggc gaattcaata tttgtcggtg attatctaat tactggtggg
     6961 caaccgaacc aattagacta cgatatgatt aatgatttag gctttgaaat tgattatgac
     7021 acttgtgaaa ataaggaaaa caagaatgaa gtttcaagag caaattgatt tattacaaca
     7081 gaaagggtta tatcggtcgc ttaaatcggt agggtatata gatagacagt atattgaagt
     7141 agaaaataag agatgtacga actatacatc taatgattat ttaggattag gtcaaatagc
     7201 gtttgataag gatgatttcg aaagatttat gcggaagtat agttatcact tatcaagttc
     7261 aagattaatt agtggaagtt cgacagctta tgaagaaatt gaaacaatgt tagcaggttg
     7321 gctcggatat agtgcatgta ctatcttaaa tagtggttat gatgctaatt tggcgttatt
     7381 taatattttc aaaaatacaa attgtgtcgt gttttcagat caagaaaatc atgcgagtat
     7441 tattgacggt attaagttaa gtggtttaga aaaagtgata tataagcatt taga
//
LOCUS       DAMQYT010000125         7429 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMQYT010000125 DAMQYT010000000
VERSION     DAMQYT010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.125"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..823)
                     /gene="rsmI"
                     /locus_tag="PGA33_001935"
     CDS             complement(<1..823)
                     /gene="rsmI"
                     /locus_tag="PGA33_001935"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDG6473739.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNI"
     gene            complement(825..1073)
                     /locus_tag="PGA33_001936"
     CDS             complement(825..1073)
                     /locus_tag="PGA33_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDG6473740.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            complement(1066..1791)
                     /locus_tag="PGA33_001937"
     CDS             complement(1066..1791)
                     /locus_tag="PGA33_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDG6473741.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            complement(2065..2412)
                     /gene="yabA"
                     /locus_tag="PGA33_001938"
     CDS             complement(2065..2412)
                     /gene="yabA"
                     /locus_tag="PGA33_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDG6473742.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            complement(2429..3232)
                     /locus_tag="PGA33_001939"
     CDS             complement(2429..3232)
                     /locus_tag="PGA33_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDG6473743.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIEDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            complement(3233..4159)
                     /locus_tag="PGA33_001940"
     CDS             complement(3233..4159)
                     /locus_tag="PGA33_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDG6473744.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQNMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            complement(4373..4702)
                     /locus_tag="PGA33_001941"
     CDS             complement(4373..4702)
                     /locus_tag="PGA33_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDG6473745.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            complement(4730..5347)
                     /locus_tag="PGA33_001942"
     CDS             complement(4730..5347)
                     /locus_tag="PGA33_001942"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDG6473746.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            complement(5349..6686)
                     /locus_tag="PGA33_001943"
     CDS             complement(5349..6686)
                     /locus_tag="PGA33_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDG6473747.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKFLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
BASE COUNT         2246 a         1480 c          983 g         2720 t
ORIGIN      
        1 aaatattata tacttcattc gttttaagtt gtcgttcttc agcaactttt ttaatagctt
       61 gttttggttt catctgtgaa gtttgaatat aatgatcaac atgctcattg atagataaat
      121 catcaaacca cgatatctca ttgttcgctt tagcaccttc aattaagata acgaattcgc
      181 ctttcaatgg tacatcgcct tgctgaatca atgcttgtaa ttgtgttaca tcatcagtta
      241 caatttgttc gaacttctta gttaattcac gccctagtga tacttgtcgt gttgcatcta
      301 tctttgcaat tgtttttaat gtatctgtca cacgatgcgg tgattcgtat ataattaatg
      361 tgctattttc atgcatacgt tgctctaata cagcactttt ttctttctct tttcgtggca
      421 aaaatcctaa aaatgtatat acatatgaag gtaatccact agccatcaaa gccgtcagcc
      481 cagcattagg tccaggcacg gtctctactt taatattagc ttctctggct gccactacta
      541 attcgtatcc aggatcacta attaagggca atccagcatc agatacgagc gcaacgtcaa
      601 gacctaattc taactgttca atgataaaag cagtctgctt atccttgtta tgttcgtgat
      661 atgactttaa tggagttgga atatcataat gattacacag tttactagtt actctagtgt
      721 cttcacaagc aatcatatca acacgtttca atacatcaac tgctctataa gtaatatctg
      781 ctaaattacc aattggtgtg cccactaaat ataatacagc catactatcg ctccttaatt
      841 aatcgcaatt tcttttgtct ggtataagtt ttaatttcat attcacgctt caatgcttca
      901 gactttgtct catacatttc ttgataaact aaatgcaccg gacgtcttac tttcgtatat
      961 ttggctcctt gacctcggtt atgtttttca acacgtgcat taacgtcttt agcgtatcct
     1021 gtatataaac ttccatcact acattttaca atatatacaa aatgactatc cataatatac
     1081 ttccttcatt tcttcgctat aattaccatc ttcattataa atataaaatg ggggcatgat
     1141 ttctaaacct tgatttccac cttttcgacc ttctactact atcgtttgtg ctgatttccc
     1201 tactttacta tatataaaaa cgactttctt aggttcaata ttcacttttc tcatttcaaa
     1261 caagacatcc attagtctct ctgcacgatg taccatgttt agcctgccac cttcttttaa
     1321 taaatgacgg gctgcaatca tgcaatcttc aagtgtacac ataatctcat gtctcgctat
     1381 cttatgtgct tctttttgat gttggtgttg ctgattctct ttaaaataag gcggattaca
     1441 cgttactaaa gtatattgtg aaggtttaaa tactttagta acgtttttca aatccatgtg
     1501 atgcattgtt aaatattcat caacatcatt gaattgaaat gtgcgtcgcg ccatatcgac
     1561 aagtgttttt tgaatctcaa caccttctat atgtcgtgga tgtttcgcaa acaataacaa
     1621 gggtatcacc ccattgcctg aacataagtc caacacaata tcttttgttc taggttttgt
     1681 aaaatgccct aacaacaaag catccgttga aaatgaaaaa acatcatcat tttgaataat
     1741 actaaaatct tctttgatta gttgatcaaa tcgttcattc tcttttaaca tttttaactc
     1801 ctatgaagac aacctttttc tattgagtaa ttgaaaactc tttttatatt atatgcctaa
     1861 ctacattcga tgtatttttg acttttaaca gctacatttt ataactaatg atactgcgtg
     1921 cacacttaat aataacattt agtaccgcca tctcttatgc aatcaccaaa actttataca
     1981 caattaaagc ctgaaacatg cattgtctca gacttatcca aaccatattc atttataatt
     2041 ataacactat ttgagtgtgc ttgattaatc acttaaaact tctaaacaga acagacaatc
     2101 ttcaccatgt cgatgttttc caaataattc gcctttacaa atatgaaatc cttctccata
     2161 caatatagcc aaattatctt tacttggtaa tggctttttc acagcttttg ctggttttga
     2221 attcgcagta tcaatagtag ttggttcatc attgcccaac acctttttca aattatcatt
     2281 ttcaagttga agcgctacat tttcttctac taattcaact gcaagtgcct ttaattctga
     2341 agtttccttt gaaagttgat tgacattcat ttctaaacgc attatttttt caaatatttc
     2401 attgcgatcc aaatgtaata atgcctcctt aatgcatagt ttctatttct tctaatttat
     2461 attcaagtgg ctgttcatgt ccctcaagct tcacctgcat agaaatgtct aatatattta
     2521 aagcaactac tttcccgtta ccatcaggcg tttcaattgc ttcaccaata tcaggtaatt
     2581 gtgcacgtac ttcctcataa tagtcatttt catattttaa acaacacatc aaacgaccac
     2641 atgcaccaga aattttagtt ggatttaatg ataaattttg atccttagcc atcttaatcg
     2701 atactggttc aaaatcccct aaaaatgtag aacaacataa cgacctacca caaggtccga
     2761 taccgccaag caatttggct tcatccctta caccaatttg tctcaactcg atacgtgttt
     2821 ttaaatgttg cgctaatatt tttactaatt ttctaaaatc aatacgatca tccgccgtaa
     2881 aattaaaaat aactttcgat ttatctaatg tatattcgca attgactaaa cgcatgtcca
     2941 aaccttgttc tcttacaatg tctttacata gtattaatgc attttcagca tctcgttcat
     3001 tacaattaaa tttatcaata tctttgtcat cagcaatgcg aataatattt ttaagaggta
     3061 acacaacatc ctcttcagca atatccatta atggattttt aacaatacct atctctatgc
     3121 ctcttttaga ttcgacaact acccagtctt ctatatctac ttgtatatca ttaggtgtat
     3181 aatattctaa ttttcccgct ttttgaaact gaacacctat tacatttggc atctaactca
     3241 cacccttaat tacgatttgt tcaaatacaa gcgttggatt tacattttga ttcaatttct
     3301 tatgtgcttc cgtcagttga tcaaacatca aaattaattg attaaacgac aacttttgcg
     3361 catattgatc aatatcattt tttaaatcac tatatatttg tttatcctct acatttacct
     3421 ttgtatgtat gatatcttcg aagaaaccat tcacagctgc aatagttaaa gattgcagtt
     3481 ttttattttt agcctgtttc aataaatcaa taatacctat aagtgccatt ggcttattag
     3541 taagcaacaa ttcacaccaa cgtataactg atttccttaa tgctaataaa tcaaattctt
     3601 catttaaagc cattgcatta tctatttgcg tagtataagt actaatcatt tcagctactg
     3661 gcttagacat gttttgttca actaatctat ttataaactt ttctttatca ataggcttga
     3721 aatatacatg ctgacatctt gaatggattg tgtctaaaat ttgctcaggt tttgtagaca
     3781 ataaaatagc aatcgtattg tccggtggtt cttcaagaaa tttcaagata ctgttttccc
     3841 cttgaacagt taacttttca aagtcttcaa tgatgtacac tttatttgtg ctttctatag
     3901 gaagttgatt catatgacgc acaagttgtt caacttgttc tttcttaatt gcattctcag
     3961 ttgttgatat atacataaag tctggatgat tatatgtact aacctttgtt tcacattgac
     4021 tatctgtttg acataaaata agctttgcaa aattaatcgc aacttgtttc atcgtttgtg
     4081 catcatcacc ttcaaataaa taggcatgcg ataatttatt tgaatgatat gcattcgtca
     4141 attgttgctg ttcatccatt cattacaaac tcccttttgc ttttatataa aaaggttgcc
     4201 aaagagcaac taattactgc aacaatagcg accatgattt gtgattattt ttagaaccac
     4261 aaaaatcaaa tctattggca tctgaactag tcgcagtcaa gcaacccaaa attgttaata
     4321 acttattaat acactatttt aagtatctct aattgatgat tgtattatag aattaaaatt
     4381 gatggaatgc atcaactggc ataacaaata cagtagcacc gccaacttca acttcaactg
     4441 gatatggaat gtacgaatcc gcactgcctc ccataggtgt aataggtgaa accaactgtt
     4501 ctctattacc acacgtttga ttaatcacag acaatatttc atctacacgg tcatcattga
     4561 caccacataa gaatgttgta ttacccgctc ttaaaaaccc acctgttgtt gccaattttg
     4621 ttgctctaaa gttattttta acaagttgat ctgcaagttc ctgactatct tgatcttgta
     4681 cgatcgctat aatcattttc attttataac acctcttcta acaattatat catatctttt
     4741 ctaaatattt gatgatagtt tgatacgtgt cttcaacaac attttcaaga ggttgatctg
     4801 cattaacgct tttgaaccgt tgtgattcat tatgaatgat ttcttggtaa ccttcaatta
     4861 ctttttcgtg aaactttaaa tcttcttgat ctaatctatt ttgatctctt gaatttttaa
     4921 taatacgttc gcgacctact tcagcactaa catttaaata aatcgtcaag tctggatata
     4981 atccatttat tgcaaattcg tttaatgctc ttacttcttc aacgccaatc cctctagcat
     5041 aaccttgata agctaatgaa ctatcgatat agcgatcaca caacacaacc ttaccttctt
     5101 ttaaagctgg tatgaccttt aatacaagat gttctcttct agatgcagca aataacattg
     5161 cttcagttct aatgtccata tcattgcctt ctaatacaat tttacgtatt tcttcaccag
     5221 taggaacacc acctggttct ctagtcataa tgacatcata atcttttact aatctatggt
     5281 aaacttcatt aattacagtt gtttttccag agccttctgg gccctcaaaa gttataaaag
     5341 ctgacatttt attcatcctc aactaaaatt ttattatttt taattccttc aactatcatt
     5401 ccagtttcca gatattcatt taccaattct atcatatttt cagttattgt ttctcctttg
     5461 aaaataatag gaatccctgg cggatacggg acaatatgtc gcgccagaac tttacctttt
     5521 gcctttttca aatcacacca agtaacatat tcaaagcgtt ttggtttata gttaccttca
     5581 gttgttaaaa gttgtgtttg tttaacttta gaaactgatt ttttcggtaa aatcatatct
     5641 tcaattttac gtaaaagcga atcaaataaa tacgtatcat catgatgcca taacggcaat
     5701 atcgctaatg cttgatagtc gtccgctaat tctaaataga tatgtgcatt cataaaccaa
     5761 ttttgaatat catgacctgt aaaaccttca tattttatca gcaacttcaa cggatcatca
     5821 acttgaagca tttcaaaacc cttcttctcc aaacattcga ttaattgcgc tctcttatca
     5881 aaaaacacgg tactatcata tgttttatag aactcggcag ctgactctaa actagccata
     5941 atcaaatacg aaggactaga tgtttggaag tagcttagat attctataat agtttctcta
     6001 taaggtgcat ttttatgaat ataaagtacc gagcccatcg ttaaagctgg taacgtttta
     6061 tgaaaagatt gaacaacata gtcagcttga taatttaatg tagaatctgg aaatccttgc
     6121 aatccaaagt gcgcgccgtg tgcttcgtca atgagtacag gaatatttaa ttggtgcaaa
     6181 aatttgataa cctcttctac attaaatgtt tcaccgtaat agttaggata agtcaacaca
     6241 gcaagtttgt gaccgtcatt attcaaacgg cttaaattaa ctttattata atgattcgtt
     6301 aacggacttt gatgcgtttc aataaaatgc ccttcttgtt ggctaatatc gagcgcatgt
     6361 aacacagatt tatgtacatt tcttgccatt aagatatcgc cttttttctg tgaaaaagac
     6421 tggatgacag ataatattcc tgaagtggtg ccattcacta agaaataagc atcataatct
     6481 gaatgtttct ccacctgctt catactttcc aaaatgactt cttcaggatg atgtaaatca
     6541 tctaatccag gtatttcagt tttatccatt gtcattgata attgagataa atgaccgata
     6601 gtcatatttt tatgacccgg aacatgcaaa gaaatcgctt cttcttgatt taaactttct
     6661 aatttattta aaataggttg cttcatgata tacgcttcct ttatttacac tgttttggaa
     6721 ttagttactt tcaaaagtat taattatata gtaacacttc tttgacaaaa gttagtgtta
     6781 cttatgcaat agcttgtcta ttgtataata attaatttcc tttttgtact tcgatttaaa
     6841 agatattaga cataaaatct aaaaacagca gtaagatgat ttatgattaa aaactatctt
     6901 actgctgttc actttttata atacttctga atgtcttcac ttatacttct agtcacagat
     6961 ttaaataatc aaaagtgcac attattaaaa tatcaatttc acactcaatg cggctcatcg
     7021 cattcatttc ttgtctagca acgttctact ctagcggaac gtaagttagc taccatcctc
     7081 gctaagaacc tttcttgact tgtgacaatc gcttgcttct ttcctctcct tcggctctcg
     7141 cttactcatt tagctctact aaactcgttg cgctcttttc tcgtttcgtc agattcaaac
     7201 gttttcactt cgccaagcca tttttctttg tgtttacttt ttattttgac gttttagaca
     7261 taaaaaaaga gacctcacgg tctcaacttg cctggcaacg ttctactcta gcggaacgta
     7321 agttggctac catcgtcgct aaagaccttt cttgacttgt gacaatcgct tgcttctttc
     7381 ctcttcttcg gctctcgctt actcatttag ctctactaaa ctcgttgcg
//
LOCUS       DAMQYT010000126         7412 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMQYT010000126 DAMQYT010000000
VERSION     DAMQYT010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.126"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(136..1140)
                     /locus_tag="PGA33_001944"
     CDS             complement(136..1140)
                     /locus_tag="PGA33_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDG6473748.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
     gene            complement(1124..1834)
                     /locus_tag="PGA33_001945"
     CDS             complement(1124..1834)
                     /locus_tag="PGA33_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HDG6473749.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            complement(1922..2128)
                     /locus_tag="PGA33_001946"
     CDS             complement(1922..2128)
                     /locus_tag="PGA33_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473750.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            complement(2194..2802)
                     /locus_tag="PGA33_001947"
     CDS             complement(2194..2802)
                     /locus_tag="PGA33_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDG6473751.1"
                     /translation="MQLFETMKIDNGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESKFETIVSPLPEKSSFTAKFQVLPKVVNPTFIKNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            complement(2802..3953)
                     /locus_tag="PGA33_001948"
     CDS             complement(2802..3953)
                     /locus_tag="PGA33_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HDG6473752.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHAMCTHSLAKDDIYAAAYSFEKAESINSTYEHQT
                     SYVSKYHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKNNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            complement(3937..4530)
                     /locus_tag="PGA33_001949"
     CDS             complement(3937..4530)
                     /locus_tag="PGA33_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDG6473753.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIR
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
     gene            4881..5549
                     /gene="queC"
                     /locus_tag="PGA33_001950"
     CDS             4881..5549
                     /gene="queC"
                     /locus_tag="PGA33_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDG6473754.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            5551..5970
                     /gene="queD"
                     /locus_tag="PGA33_001951"
     CDS             5551..5970
                     /gene="queD"
                     /locus_tag="PGA33_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDG6473755.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            5974..6687
                     /gene="queE"
                     /locus_tag="PGA33_001952"
     CDS             5974..6687
                     /gene="queE"
                     /locus_tag="PGA33_001952"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDG6473756.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
     gene            6786..7370
                     /locus_tag="PGA33_001953"
     CDS             6786..7370
                     /locus_tag="PGA33_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473757.1"
                     /translation="MIVYILINIAIVVLITGFNLYRHQMQHLSLSAMLLSITINAFIN
                     TFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVT
                     DFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNSLDETAENNNKIKKLMIKGLIIINI
                     SFIVMMVLTPYWYLYLIVYLIFLLFLLWQKVYKF"
BASE COUNT         2435 a         1271 c         1092 g         2614 t
ORIGIN      
        1 acactttatc ttttcatttt actgtgaaac gaattagaaa atgatacatt tcgcagataa
       61 aacacagtat tatttcatta tacattagga ggaaattatt tgcatcggac tcgagtatga
      121 gctacgtacc aaatattatt tcatcatttc taattgcatt ctatattcta ttccatggaa
      181 taattggttt aaccagttac tatacttaac aaggttcttt tcagccgtta aaatatctat
      241 aaattgaaat ttcaaacgtg atccttgcgg tttttgtgct aatttcgtta aatgataact
      301 tgcgattgta ccgatttgcg ggtagctacc tagcgtataa tgatcattta ataggataat
      361 aggtgtgcca tcacgcttaa tttgtatcgt tccttttttg acagtctgat acggtggcat
      421 atcttcataa aaagctttga ttttttcacc ttcaagcatc atccccatgc gatttgcttt
      481 gctcgttact ttataatcat gtttcacaaa tctttgaatg gcatcttctt taaaatcttc
      541 cgtaccttta tttttaacga catgaaatac atcagacata taattaaatg acaaggcgta
      601 tccatcaata ccccaatctg tttgtttcgt gtgagcaagg ttttcaaata acttatgatg
      661 acgagctgta taatctctct taagctttat ttcatcgcca tcttgtaatg ttctaccttt
      721 aaaaccacca atttttacat taaagtcggt tgagttagat cctaaccatg catctaattc
      781 aaatccgcct cccacagcta aatacactcg cgatgtataa cttgtttctc taaactttaa
      841 aacatcgcct ttatctaaca aatacaattt atatggagat atagtcatat gctcagtgta
      901 agctttgaca ttaccccctg ctaatgcaat cagcgtaggt tctgtaaaac gaatcgttgc
      961 cattttatta gtcatttcca aagttgcttc attcttgtca tttgcaacta atcgattagc
     1021 aatttcatgt gctaaagtat caagtgcacc acatggaatt acaccatcat gttcatatcc
     1081 ccttctgcca aagtcctgaa agctactgaa taagccactt ttttcaatta tgattgacat
     1141 ctttaaacgc tcctacttct aattcatttt cattgatagg tctaaattta atattatcgc
     1201 ctaactttaa gcgtgcgaaa tctgattctt tcggattaaa aagtgataat ggtgtataac
     1261 caataactaa ccaatcatta attgtatccg tagttacaat accgcatttt ttcccttcaa
     1321 gtactacaga accagctgga atgaattttt tctgtttact cgtgtgatta atatacaatt
     1381 tcttatttaa tccagttaaa taaggaaacc caggtgaata tcccatcatc gaaacaaaat
     1441 attgcgcctt agaatgtaat tcaataaaag tttctagctt gattttgtaa tgttttaaaa
     1501 gtgattctaa atccggacca tatttcgaac catacacaat tggtatttca atcaaatctt
     1561 gctggttaaa atcatgtttt atgtttaaat gaatcgattc tattagtgct ttcatgtata
     1621 aaaaaggtga ttgtatatta tggtgtttaa tcatatccct tgcgtcatag acaatcatca
     1681 tgtctgattc tgatggcaca atttctataa taaatggata attttgttca attaaatatg
     1741 agcgaagtgt taataaatct tcggttaaac ttttagatac ttctttttca attgcgacta
     1801 caatggcttg gtcaccttga ctatatattt tcatttgtac acctcttcaa tatttggtta
     1861 ctttactttc atttttaatt atagaatcga ttgaatagtt cattttacct actcactaaa
     1921 tttactggtg gtacattaaa tccattgccc gcacaacaaa aggcataagt tcgtgaatta
     1981 atgcgtatac aaggataaag cttataacag tagtaattgt tgctatcaaa cgaacaacat
     2041 atattctatt ttcagatagc aatcgattca ctaatcgata atttacgtat acaagaatta
     2101 acaacagcac aatataaaca ataatcatat ttggccccat ttaattttta aatttgtttt
     2161 cacatcattt tctactttat tatcatacta attttaaagg caaaggtgga catcggcaac
     2221 ctctcgtaaa ctatttatca aaaataaacg tatctcattg ttatgatatt tataaatcaa
     2281 ttcgttttta ttaaagtctt tttcaacaag tttgtcacta tctattaaat aatcacgcat
     2341 gcatcctttt aagaaatcat ctttataact tggtgtgtac cattttccat cctcttcaat
     2401 gacaatgttg ccaatatcaa attcaaggac cttgccgtcc tctgaagtta gcaatattaa
     2461 atctgtttca tgattgtgtg ctaaatgctt tcgttctgtc gttttatttt ttataaaagt
     2521 tggattaact actttgggca acacttgaaa ttttgctgta aaactacttt tctcaggtaa
     2581 aggtgacact atcgtttcaa atttgctttc agcatttaat acgattttaa gtctatattg
     2641 accactgtga tactttgttg ttacatcgtt taattcattt cgccatgcat gttcatcaaa
     2701 tttaaagttt aatcgctcag aagaacattt tatgcgatta gtatgataag taagtctagg
     2761 gatatgtcca ttatcaattt tcattgtttc aaataattgc attataacat ctccaaaatc
     2821 ttggtttttg cgtaaaattc attcacttca tcttttggct tagaatcaat tgtaatacct
     2881 gctccgactc cataaatcgc ttgtccatat ttatactcaa tagtgcgaat cggaatatta
     2941 aaaatcattt tatcatcttc agttggaagt aatagtccaa ttgctccgca gtatatacca
     3001 cgaggtgaac tttctaattg tttaatatat ttcattgtat tcagtttcgg tgcacctgta
     3061 atcgaaccac aaggaaataa cgatgttaaa atttgagtta agtctgtatt attttttaaa
     3121 gttccactta ccatcgaagt catttgaaat acagttttat atgcctcaat aaaaaatagt
     3181 ttatacacct taattgagcc actctgtgat attctcccta tatcgtttct tagtaaatca
     3241 acaatcatga cattttctgc acgatctttc gaagaagttt gcaatgtttt ataatactgt
     3301 tgatcttcag cttccgtttt acctctaggc attgtccctt tcatcggttt gcttatgata
     3361 acgttatcga cattgttaaa ttgtcctttt tgaaaaaata attctggtga gatagacgct
     3421 acttggattt catcagtttg taataacgca gtataattac cattactaaa ttgagttaat
     3481 cgttcatata aagtactaat aggataataa atgttatccg ttaagcgcgc cgtatagttc
     3541 acttgatacg tttcgccttc aacaattgct tgttggacac gtttaatatt agtcatcata
     3601 acctcagaag attcaacaaa tgaaaaatga tactttgata cataagaagt ttgatgttca
     3661 tatgttgaat ttatgctttc cgctttttca aaactataag ctgctgcata aatatcatct
     3721 ttagctaatg aatgtgtaca catagcatga ttaaaatact ttgccgcttc gtaacttaaa
     3781 tataacgaga catatctacc ttgtcgttgc tgcgcttgtg caaagtgtat cacttctccc
     3841 acatcagcca acttagtagc aacatacttc tttataaatc cctttaattg aatatggtat
     3901 tgcttatatt cattttcagt taaatagtag cgataattat attctattct cacagtaatc
     3961 acctaccttc gataaaaata attcaacttg tcgataaccg tattcactca aaatagattc
     4021 aggatgatat tgcacaccaa aaaccggaaa tctaatatgc tcaaatgcca taataatcgc
     4081 ttcatcgttt tttgctgtaa tctttaagca atttggaaaa gtcgctccgt cagcaattaa
     4141 tgaatgataa cgcattacat tgaaattttg aggcagtcct tgaaaaatac cttcattggt
     4201 atggcgtaac tgtgtagtat gtccgtgtac aggatgatag ccgtgaatga tatttccacc
     4261 aaaataagac acgatacatt gaaatcctaa acatacacct agtataggta cacgctgata
     4321 aaattgttct aacacttcat tcaagatagg ataatcatcc ggattacccg gcccaggcga
     4381 aataacaatt gcttttggct tcatattaat gacgtcttct atcagcagat tatcaatacc
     4441 aacaacttga actgttagtt tcgtttgagt cttaatatag tctattaaat tatatgtaaa
     4501 tgaatcatta ttatctatga ctagaatcat tgtatactcc gttctaaatg tgttttattt
     4561 ttataatatg tattggatgt agctaaaact ttaaaagcat tgtcattatc ctgaccttga
     4621 ttttaactaa tatatggtat attctattca tcgtacataa atgaatatca gaggttccta
     4681 gctgaaaccc tctataaaaa actagacatt gaaatttcaa acattcgttg gtaaaccttt
     4741 aatatatatt ataggagcaa cccatttctt tcgtctatct tttatggaga taggcttttt
     4801 ttattttata aattaaaaac gggcttatca atgccattaa cctctttatt aatattatta
     4861 atgataggag caattacaaa atggaaagtg tattaaataa tgaaaaagcc atcgttgtct
     4921 ttagtggtgg tcaagacagt acaacatgtc tcttttatgc aaaaaaacat ttcaaagaag
     4981 ttgaactcgt aacgtttaat tatggccaaa gacatgatac tgaaattgaa gtcgcaaaac
     5041 aaattgcaca agatcaagga atgaaacatc acgttttaga tatgtcatta ttatcacaac
     5101 ttactccaaa cgcattaaca caacatgata tggaaattac taataatgaa gatggtatac
     5161 ctaatacatt tgttccagct agaaatttac ttttcttgtc gtttgcaggc gctctagctt
     5221 atcaaattgg ggctaagcat attattacag gcgtatgtga aacagacttt tcaggctacc
     5281 cagactgtcg cgatagtttt ataaaatcaa tgaacgtaac attaagccta gctatggaca
     5341 aagattttgt cattcatact cctttaatgt ggttaaacaa agcagaaacg tggaaattaa
     5401 gtgatgaact cgaagtttta gattatattc gtacaaaaac attaacatgc tataacggta
     5461 tcattgggga tggctgtggt gaatgtccag cttgtcattt acgtcaacgt ggactaaatc
     5521 aatatcttga aagtaaagga gcgctttaaa atgttacaac aaatctatcc tagtacaacg
     5581 catccatatc aattcgaatt aaataaagat tttaattttt cggctgcaca tcacattcca
     5641 tgtgaagaag caggtatttg tcaaaatgtc catggtcata cttactttgt taatttaaca
     5701 attgtcggtg ataaactaga tgacactggc ttcttagtga actttagcca tttgaaaaag
     5761 atgatacacg gtaaatttga ccatcaactg ttaaataact tacctgcttt taaaaacaaa
     5821 atcccttcaa ctgaaatcgt agcggaaaca atttatcaaa ttgttaaaga aaatttggca
     5881 tcgctcgaac accaaccaaa atgtattcaa gtatttgtaa gagaaacacc aacaagttat
     5941 gtcgtattta gaccaaagga acaggtgtaa ctaatggcta aaattcctgt actcgaaata
     6001 ttcggtccaa ccattcaggg tgaaggtcga gtaatcggta gaaaaacgat gtttgtgaga
     6061 acagctggat gtgattatcg ctgtagctgg tgtgattctg catttacttg ggatggtagt
     6121 gctaaaggcg atataaaact catgaccgct gaagaaattt atgatgaatt aaaacgaatt
     6181 ggtggcgatt tatttaacca cgttacaatt tctggtggta acccagcatt aattaaaggt
     6241 atccaagagt tagttgactt atttcaagat aaaggtattt tcagcgcact ggagacacaa
     6301 ggcagtaaat tccaaccttg gatgacacaa attgatgatt taacgattag tccaaaacca
     6361 ccaagttcaa ctatgacacc tgatttaaaa aagttagatg aagtgattac acaatgcgta
     6421 ccttcatcat taaacttaaa agtagttgta ttcgacgata aagattatga ttttgccaaa
     6481 atgatacacc accgttatcc agatattccc ttctatttac aagttggtaa cccatattta
     6541 tcagacagcg tagataatca taccgaaaag ttgttagaac gctacgagca gttggtggat
     6601 ttagttatgc aaagtaatga tatgaatcac gtttatgtgt taccacaact tcatacatta
     6661 ctttggagca ataaaaaagg tgtataaagg ataacttatt ttactttttg caagtcatat
     6721 ctaatgaata acaatatatt aattgatata atagttgatt gaagtataac gaacaggagt
     6781 ccatcatgat tgtatatata ttaattaata ttgctatcgt cgtacttata actggattca
     6841 atttatatag gcatcaaatg caacatttat cattaagtgc aatgttgtta tctattacga
     6901 taaacgcctt tataaataca ttcataattg ataaatataa ctttattact ctgtgtacta
     6961 taacgatgtt tatcatttgg acgatactac agttttacat tgataaaaaa ttaaaacctg
     7021 tatacattac agaccaaaaa tttattgcta tcatattaac gattgttgtc agtttaacgc
     7081 aacgtgttac cgacttttca agtacacaat caatatatat gtctattcca tttttagccc
     7141 ctgccatttt catcattggt ggtattatgt tgtttatttc aacatttaat agcttagatg
     7201 aaactgctga aaataataac aaaataaaga aactaatgat taaaggactt atcattatta
     7261 acatttcatt tatcgttatg atggttttaa caccatattg gtacttgtat ttaatcgtct
     7321 atcttatttt cttgttgttt ttattgtggc aaaaggttta taaattttaa taccaaaact
     7381 attaaacact tctgatattc ttagttcaaa at
//
LOCUS       DAMQYT010000127         7406 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMQYT010000127 DAMQYT010000000
VERSION     DAMQYT010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.127"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..313
                     /gene="mnhE1"
                     /locus_tag="PGA33_001954"
     CDS             <1..313
                     /gene="mnhE1"
                     /locus_tag="PGA33_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HDG6473758.1"
                     /translation="IIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKD
                     WQIVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGE
                     I"
     gene            313..606
                     /gene="mnhF1"
                     /locus_tag="PGA33_001955"
     CDS             313..606
                     /gene="mnhF1"
                     /locus_tag="PGA33_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HDG6473759.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            584..940
                     /gene="mnhG1"
                     /locus_tag="PGA33_001956"
     CDS             584..940
                     /gene="mnhG1"
                     /locus_tag="PGA33_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HDG6473760.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            1189..2343
                     /locus_tag="PGA33_001957"
     CDS             1189..2343
                     /locus_tag="PGA33_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDG6473761.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKELDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(2399..2773)
                     /locus_tag="PGA33_001958"
     CDS             complement(2399..2773)
                     /locus_tag="PGA33_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDG6473762.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHRGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(2792..4108)
                     /locus_tag="PGA33_001959"
     CDS             complement(2792..4108)
                     /locus_tag="PGA33_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDG6473763.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(4519..5994)
                     /locus_tag="PGA33_001960"
     CDS             complement(4519..5994)
                     /locus_tag="PGA33_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HDG6473764.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVFYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSEVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(6125..7333)
                     /locus_tag="PGA33_001961"
     CDS             complement(6125..7333)
                     /locus_tag="PGA33_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDG6473765.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
BASE COUNT         2437 a         1357 c         1083 g         2529 t
ORIGIN      
        1 tattattaaa ttagttatta ttttcttaat cgaactgatt aaagctaatt ttgatgtttt
       61 gaaaattatt attaaacctt cgattaaaaa tgaaccagga ttctttgttt atcacacaga
      121 tttaaaaaaa gattggcaaa ttgtcttgct gtctaattta attacattaa cacctggtac
      181 tgtagtgctt ggtgtaagtg acgatcgtac aaaaatttat attcatgcca tagatttttc
      241 aactaaagag caagaagtag aaagtattaa aacatcatta gagaaaattg taagagaggt
      301 gggcgaaata taatgaatca taatgttatt atcgttattg cattaatcat agttgtcatt
      361 tctatgttag ctatgctcat tcgcgttgtg ctaggcccat cacttgccga tcgtgttgtc
      421 gcattagatg cgattggtct tcaattaatg gcagttatag cattattcag tattttatta
      481 aatattaaat acatgattgt cgttattatg atgattggta tattagcttt tttaggtact
      541 gcagtattct ctaaatttat ggacaaaggt aaggtgattg aacatgatca aaatcatact
      601 gattagtctt gcacttatct ttgttatcat cggtgcttta attagcgccc tagcagctat
      661 aggattattg agacttgaag atgtatattc acgtgcacat gctgccggaa aagcatcaac
      721 attaggtgca atgtcattac tatttggtac gtttttatat tttattgcta ctcaaggttt
      781 tgtaaatatg caattaatcg ttgcgattat ctttgtatta attacaggtc ctctttcaag
      841 tcatatgatt atgaaagcag catataatat taaaacgcct tatactaaaa agactaaagt
      901 cgatgaaatt tcggaagact taaaagacac aaaattgtag attatataac aaaatcatga
      961 atataaatca acaacaaaca gcagtaagat gattccaaat taggaatgat tttactgctg
     1021 ttttcttttg acattgttac ctctttttca atgatttttt ctttgactac agattcgccc
     1081 tatctacata tatctcttta atttaattgc ctttcatgtc gttatgtatt atgataataa
     1141 taattataaa tcgtaacgat tacgttttaa aaagagagag gttttattat gcattggaca
     1201 attatcggcg gtggcataca gggaactgca atcgcacaaa aactattatc aagcggatta
     1261 acaacagacc gattaacaat cattgaccca cacgaaactt tttgccaaag gtttaactca
     1321 tatacaaatc gaatagaaat gccttattta agatcaccga ttgtacatca cgtacatcca
     1381 caaccgttcc atctaaaaca attcgctaaa cagcaccaat atacaaatgc tttttatggt
     1441 ccttatcaac gacctgaatt gacaatgttt atggatcata ttgcacatgc ttctaaacaa
     1501 tatcaattag aggattgctt ggttcaaggt ttagttcaaa ctttagataa acaagaagac
     1561 aaatggcata tcaagttaga agatggacaa attatcacta cagattgcgt cgttattgca
     1621 ataggcagta caaatattcc gtttatgcct gacattttaa aagacaaaca gaatgtaaat
     1681 catatcttcg agaaagaact tgatcaagta gtatatgata agaccgatca tatcgttggt
     1741 agcggcatta ctgctgcaca tcttgcactt aaattgttaa atcatgataa cgataaaaag
     1801 attcatttat ggctaaataa agatattgaa atacatgact ttgatgctga tcctggttgg
     1861 ttaggtccga aaaatatgtc ttcattttta agtactaaaa gcatgcctga aagaaatgcc
     1921 attgtacaac gcgaacgtca taaaggatca atgcctcacg aactgtactt acgccttaaa
     1981 aaacatatta aaaatggtcg tataaatgtg cataaaacac ctatcactca aattagtggt
     2041 ggtgtaatta acactgaaaa tgattctgtt ccatatcaac agattatggt tgcaactggt
     2101 tttgaacaag attttatgtc acaaccactt attaagcaat taatacaaaa ttatgatgca
     2161 cctatcaacg aatgtaatta ccctgttatt tccgaaaaat tagaatggat accaaatcta
     2221 tttgtcgcag gatgctttgc agacttagaa ttaggaccat ttggtagaaa tgttatgggt
     2281 ggccgtaaag ctgccgaacg cattgaacaa gcatttctaa aactacaaca atatagcgca
     2341 taaaaaaact gctagccgta gttatgaata tctcataaca tttcagctag cagttctttt
     2401 attttaaagg tttaatagca actgtaccac gcataactgt aattaattgt tctttgtcat
     2461 tcttaatttt tatatcccat acatgtgtcg acttacctcg atgaataatt tcagctgtcg
     2521 cagtaacacg accatcttta gcagaatgaa tatggttagc attcatctct aaacctaatg
     2581 gaataaattt ggttgtatca attaaattag cagatcctaa tgaacatgct gtttcaccta
     2641 aagcaatcga agccccacca tgtaaatatc caaatggttg ttttacttta tcagtaacag
     2701 gcatagaaat aacaactaaa ccatcttcct ggtgatctat tgacatctca aatgtctcta
     2761 ataaatgagt catattttca cctctttatg cttatagtac catagcagca atagtaccga
     2821 aaatcattaa aggaatatta taaaacaaga agtttggtac acatgtatca cgtatatgat
     2881 catgttggcc atcaacattt aatcccgcag ttggtcctaa tgttgaatca cttgcaggcg
     2941 aacctgagtc acctaatgca ctcgctgttc caatcaatgc gattaatgcc attgtatcta
     3001 gtccaattga cgctccaaaa ggaatgaata atgatgcgat aataggaatt gttgcaaatg
     3061 atgatccaat acctaaagtg acaattaaac ctatcacata catcatgata atgctaaata
     3121 atttattatc accagtaata cttgttaaag ttttaactaa ttcatctata tcaccagtag
     3181 cattcattac accagcaaat ccatttgctg ttaaaataac tacaccaata taagccataa
     3241 ttttaatacc ttcaacaaac ttagcatcta attcatacca attatatgca cgtgaaataa
     3301 agaatacgag tacccctgcc agtgcaccaa aaatcattga atctgtaaat gtttgtacta
     3361 aaaatgtagc tagtattgct acaattgtta ctattaagat atatggtttt aactctgtaa
     3421 cattgtcact atctgaaatt ttacgtgttt catattcacg tggtttacga tatacatata
     3481 aaccgataag taagccaaca atatacccca ttgaaggaat aagcattgct ttccaaatca
     3541 tattaaactc aattgggtga tttgcctttg caaagccact ttgaataatt tgctggaaaa
     3601 tttgaccgaa tccatatggt aataacacat acgggaaaca taaaccaaaa ccgataatca
     3661 aaccgattaa acgtctatct atttttaagt cattaaacag acttaacaat ggtgggatga
     3721 caattggaat gaatgcaata tgtacaggaa ttaagttttg actcatgata ctcatagcta
     3781 ataatgcaat gattattgtc actttgactt taactcttga ccatcgacta ttttcagcgt
     3841 gaattgcatt tataattttt cctactaaat agtctgtgat accactgtat gaaattaatg
     3901 ctgcaaatcc acctaataaa gcatagctta atgctacctc agcaccatcg actatatttt
     3961 tcccaaatac atttataact ttttcaatgc tcatgcctga aattaagcca ccaactagcg
     4021 cactgataaa taagcttata actacgttta atcgacataa acatagcaca atcattaaaa
     4081 ttactgctat tactactgca tttatcatat gacaaccatc ctttatcact ctatcgaact
     4141 aaagcgttta tagactacat agtatcagta gctttaacac ctgtcaatag caaactatac
     4201 caataatctt tattttttag ttaattgtct ttcttaaaat aattttagct ttcattaaat
     4261 taaacaattt tacaagcttg gaacaccaat caaaatccta agttctaaaa tgcaatatta
     4321 gtagtcgttg actgaatgaa catatgctta taatattttt ttgcaatgct agtcaagttg
     4381 atttatgctc acaaggatat gcgatttata ttttcttaca acaatgaaaa tgcctgatac
     4441 aatgcgatcc tttatacttt caagagttaa attagaaatg tatatcaaga cacaagttgt
     4501 atcaggcaaa taattaaatt attgttgttt taaccattgt actatcgtcg gaatcataaa
     4561 tcctgttggc ccttttggac cattatatga agctttatta gtcgttgctg gacctgcaat
     4621 atcgaaatga atgtgaggtg tttgaccact aaaatgtgtt acaaaacttg ccgcaaatag
     4681 cgctttacct tgtccattcg tatggttaac taaatcagcg atatcactgt gtttaatact
     4741 tgcacgttcg gttgcagtaa tcggtaattc aaataccatt tcatcgactt cagaacttat
     4801 ttgtaatata tcgtttaata tcactttact attcgattca aatgcagcag ctttatcatc
     4861 gcctagtgca acaattgctg cacccgttaa tgtagcaaag tccataatca cactaggctg
     4921 atattgattt gcataaaaca cagcatctgc aaggactaat ctaccttcag cgtctgtatt
     4981 cattacttct acagtttcac cacttaatgc tgtaaataca tcatctggct tcattgatgc
     5041 ttcatttatc atattttcag cacacgcaag cactccgaca atatttacag gcagttgtaa
     5101 acgactagcc gcttcaatga taccaacgac attcgcagcg ccacacatgt caaacttcat
     5161 tgtagccatg ccattcttcg ttttaatact ataaccacca gaatcatacg ttataccttt
     5221 accaactaag gcaattggtg cttcatcttt gtctttgcca ttatatgtga tggttactaa
     5281 tctcggttta tgcttactac ctttacctac tgcttgtaaa agtccgaatc cttcagaaac
     5341 taaagtgtca taatctttaa catctacttt gacctttgta tttttaaaat gattaacaat
     5401 atcttctgca aatgtttgtg gtgttaatac attcggtggc atattactaa agtctcttgc
     5461 caaattaatg gattggccaa tactgatacc ctcatgaata aaatctaatt caattaatga
     5521 ttcactaata aggtttaaat tcgtcttaaa cggtgccttc ttacttgatt tataatgatc
     5581 gaattcatat gttgcacgct cactttgaat accgcatgcc attaatacat ctgataactg
     5641 atcatatttt gaaataaatg aatccataag taaatacgta tcttcaatgt gttctgactt
     5701 tatgtattgg aaaagatgtc cccatatttt caacatatct tgataactac gtgtctttaa
     5761 gtttcctaaa ccaactgtaa ttaaacgata tgtttgatct tgtacatcaa atgcagttgt
     5821 ataaatcttc ccaactttac taccaataat atgttgatgt tttagtcttt caagtgattc
     5881 tgtaatatcg atatgattaa aactaatgcg ctctaactga tttaaatgtt ctggtatacc
     5941 aataatcaat gtatttattt cgttgcttag tgtgttattt aatttaaaat tcataatgta
     6001 cctccttaga tttgatatgt tactcacttc aaattgtaca acaataaagc ccttcagtga
     6061 cactgaagga ctttataaat gaataattta attttatgtg tttagctgaa actttattta
     6121 aagcctagaa tttacctttt ttgaatgcta aaccgatacc accgattttg aataccgcac
     6181 gtgtatcaat cacttttttc atgaatgctg cttttttacc agcgataggt ttaccaaata
     6241 ccatacctac accgtcatgt gaacctaaag aacaaacagt tccacgatca acgtattcga
     6301 attcttcagt tgattcaccg tttaagatgc gtttaatgtt tttagcaaca ctttcacctt
     6361 gttgcattgc aatttgtgct gtagttggta atggacgttc ttctccagct gggataaacg
     6421 ctgaacagtc accaataaca aaaatgttgt cgtaaccatt gattgttaaa tcttgctttg
     6481 taacgatacg tccacgttta acgccttcaa atgattcttc cattaattta ctaccacgta
     6541 caccagctgc ccatactgaa gtacctgcat ttaattgttg tttttcacca tctacttcaa
     6601 ctacaaaacc tttttcgtta caagcaacga ttggtgtagc aattttaaat tcaacaccgc
     6661 ggtcttctaa gtagctaact gcgtggttaa ctaattcttc tgagaacatt ggtaacattt
     6721 taggtgctgc ttcaacacaa gtgattttaa ctttattttg atccacacca tatttgctac
     6781 ataattcagg aattctgtct gttaattcac ctaagaattc aacaccagtg aatccagcac
     6841 caccaactaa gatagataaa tcgttatcat ctttttcttt tgatgctgca tagttagcaa
     6901 atttgtcttc gatatgacgt gataattcac gtgctgtgat aacattttca atttggaaag
     6961 catgatcttt cataccttcg atgccgaatg tttcactaac gaaacctaat gctactacta
     7021 aaatatcaaa gtcataaata ccttgatttg tttctacctt tttagcatca cggtcaattt
     7081 ttgttacttc tgcttgaaca aagttcactt tgtctttctt caagacactt tccacaggat
     7141 ataatacatc ttcatagttt agtgtacctg ctgatgcttc atgtaaccat gttgcttcat
     7201 agtgatattc atttttatta ataagcgtaa tttctgcttc ttctgttgat atcgcttttt
     7261 gcaatttagt tacagtttgt aaacctgcat aaccagcacc aagtacaagt acttttttac
     7321 gatcttgagc catttaattt cacctaagct ttcatatttt tttaaccaaa tgctgataat
     7381 tactagtaac aactggtcat tactac
//
LOCUS       DAMQYT010000128         7393 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMQYT010000128 DAMQYT010000000
VERSION     DAMQYT010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7393
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.128"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..300
                     /locus_tag="PGA33_001962"
     CDS             <1..300
                     /locus_tag="PGA33_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDG6473766.1"
                     /translation="NWEYFKVQINQKLSEPETKNIYSQRKIDVEPAFGFMKAILGFTR
                     MSVRGINKVKRELGFVLMALNIRKIAAQRAVHYKIHIKKADFYQIINRNQLFYIA"
     gene            complement(333..1694)
                     /locus_tag="PGA33_001963"
     CDS             complement(333..1694)
                     /locus_tag="PGA33_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDG6473767.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
     gene            2042..3163
                     /locus_tag="PGA33_001964"
     CDS             2042..3163
                     /locus_tag="PGA33_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDG6473768.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            3212..4480
                     /gene="icd"
                     /locus_tag="PGA33_001965"
     CDS             3212..4480
                     /gene="icd"
                     /locus_tag="PGA33_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDG6473769.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVENEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            4870..5574
                     /locus_tag="PGA33_001966"
     CDS             4870..5574
                     /locus_tag="PGA33_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDG6473770.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            5574..7235
                     /locus_tag="PGA33_001967"
     CDS             5574..7235
                     /locus_tag="PGA33_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDG6473771.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKKIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
BASE COUNT         2668 a         1062 c         1378 g         2285 t
ORIGIN      
        1 aattgggaat actttaaagt ccaaattaat caaaagcttt ctgaaccaga aacgaaaaac
       61 atctatagtc aaagaaaaat tgatgtagag cctgcttttg gatttatgaa ggctattttg
      121 ggtttcactc gaatgtcagt tcgaggaata aataaagtta aacgagagct aggttttgta
      181 ttgatggcac ttaatataag gaaaatagca gctcaacgag ctgtacatta taaaatacat
      241 atcaaaaaag ctgatttcta tcaaataatt aatagaaatc agctttttta cattgcctaa
      301 gaacttaatg tcccaagccc taaaacttgt tgttatttat ttgatttagc agcgatacgt
      361 ttatatctta agtacataaa tgctaaaagt ataaaccaaa tcggaataaa ataaattgca
      421 cgtcttgtat caacattaat aaataataac ccgaacacaa aaatgaagaa tacaaatatt
      481 aagtagccca tatatttgcc acctaatagt ttgtacgtag catttttatg tagatctggg
      541 tttttacgac tataattgat atatgcaatg ataatcagac cccatacaac taaaaataac
      601 actgtagaga tggtagtcac atacgtaaat acttttgtcg catctgggaa aatatagttt
      661 agtaatgctg ccacaagtaa taatgctgat gaagcaaaga ttgcaacatg tggaacgcca
      721 tatttattcg tcttagaaaa gttcggaggt gcttgttgtt gacttgataa accgaaaagc
      781 atacggctat ttgagaatat accactgtta catgatgaag cagcagcggt taatactaca
      841 aaattaatca agcccgcagc aaacggaatt ccgatcaatg cgaataattt tacgaatgga
      901 ctgttatcag gatcaacttg ctgccaaggg ataatagaca tgataaccgc taacgcccca
      961 acgtaaaata ttaaaattct aatgggtaca ctgttaattg cttttggaat tgttttaact
     1021 ggatcttttg tttccccagc tgtaacacca atcatttcaa ttcctacaaa tgagaatagt
     1081 gccatttgga aagacataaa gaatccagaa gcacctttag cgaatatgcc gtgttcatat
     1141 agatttgtga aactggcatg cccgaattga gttttaaatg caaatagaat catgacgaaa
     1201 ccaactacta ttaaaccaat aattgtcgct attttaatga tagagaacca aaattctaat
     1261 tctccgaaaa gtcttgcgct aagtaggttg aatgacatta ataacagtac acaaaatagt
     1321 gcacttatcc agtttggaat ttctgggaac caaaagctaa catattttgc cacagccgtt
     1381 acttcagcca tacctgtaat aatccaacag aaccagtatg tccatccggt aacaaatcct
     1441 gcaaaaggcc caatatatgt attggttaca tctgcgaaag atttaaattc agtattctgt
     1501 ataatgattt ctcctaaacc tcgcataaac ataaataaca taaatcctat aatgatgtat
     1561 gttaatagaa ttgaagggcc ggttaatgca atcgtttgac cagcacctaa gaataagcct
     1621 gtaccaattg caccgccaat tgcaattaat tgtatgtggc gattgctcag ttccctttgt
     1681 aatttttcag ccataataca tctcccttaa atatagatat gtttattatg cacttatatt
     1741 gagatatata caattatttt cggtaaaaat gtgtaaaatt ccatgttaat atactttggt
     1801 ttttataatc atatataata accaattgaa aatttaattc tattgtaaaa ttcatggatt
     1861 attcacatct tgaaaaagct ttaatggtgc tatttgtggc tattctgtga catttacata
     1921 gatttacaaa aaaattgttg cacatataat gccagttttt atatttcaca aacgaaatgc
     1981 gtttagctat aatattagtt gaaagccatt tcataaagaa acagtaaagg ggaaatttat
     2041 catggcagaa ttacaaagag gtttagaagg ggttatcgca gcggagacta aaataagttc
     2101 aattattgaa agtcaattga cttatgccgg ctatgatatt gatgatctag ctgaaaatgc
     2161 gcaatttgaa gaagttattt tcctattatg gaactataga ttgccaaacg aagaagagct
     2221 tgctcatcta aaagggaaat taaatcaata catgacatta aatcctcgtg tgtatacaca
     2281 ttttgaggag tatgttacag atcacgtgca tccaatgaca gcattacgta cgtcattatc
     2341 atatattgca catttcgatc ctgatgctga aaatgaatca gatgaaaatc gttatgaaag
     2401 agcaatgcgt atacaggcta aagtagcatc attagttaca gcgtttgctc gagtaagaca
     2461 agataaagaa ccacttaagc ctaatcctga cttaagttat gcggcaaact tcctatatat
     2521 gttacgtggg gaattaccaa cagatataga agtagaagcc ttcaataaag cacttatttt
     2581 acacgctgat catgagttga acgcatctgc atttacggca cgttgtgcgg tatcatcatt
     2641 gtcagatatg tactcaggta ttgtagcagc cgtaggttct ctgaaagggc cattacatgg
     2701 tggtgcaaac gaacaagtta tgacgatgtt atctgagatt gggtcaattg aaaatgttga
     2761 tgcttactta gatgaaaaat ttgctaataa agataaagta atgggcttcg gtcatcgtgt
     2821 atataaagat ggtgatccta gagcgaaata tttaagagaa atgagccgtc aaattacgaa
     2881 agacgctggt cgtgaagaat tatttgaaat gtcagtgaaa atggaaaaac gtatggcaga
     2941 agaaaaagga ttaattccta atgttgattt ttatagtgcg agtgtttatc actgtatgga
     3001 aatacctcat gacttattca cgccaatctt tgctgtaagt cgttctgcag gatggattgc
     3061 tcatatttta gaacaatata aagataatag aattatgcgt cctagagcga aatatattgg
     3121 cgaaacgaat cgtaagtata tcccgcttga agaaagaaaa taatcaatac aaattaaaaa
     3181 tgaagatgta aaatttggag gtaaaataac tatgactgca gaaaaaatta ctcaaggaac
     3241 tgaaggatta aacgtaccta atgaaccaat tatcccattt attatcggtg atggaattgg
     3301 accggatatt tggaaggcag caagccgagt tatagatgct gctgttgaga aagcctataa
     3361 tggcgaaaaa cgcattgaat ggaaagaagt gctagctggc caaaaagcat ttgatacaac
     3421 tggtgaatgg ttacctcaag aaacacttga tacaattaaa gaatatttaa ttgctgttaa
     3481 aggaccttta acaacaccaa ttggtggtgg tattagatca ttaaatgtgg ctttacgcca
     3541 agaattagat ttatttactt gcttaagacc ggtacgttgg tttaaaggag taccatcacc
     3601 tgttaaacgt ccacaagatg ttgatatggt tattttccgt gaaaatactg aagacattta
     3661 tgctggtatt gaatttaaag aaggtacaac agaagttaaa aaggtaattg acttcttaca
     3721 aaacgaaatg ggtgcgacaa acattcgatt cccagaaact tcaggtattg gtattaaacc
     3781 agtttctaaa gaaggaactg agcgattagt tagagcagct atacaatatg ctatcgataa
     3841 taaccgtaaa tcagttactt tagttcataa aggtaatatt atgaaattta cagaaggctc
     3901 atttaagcag tggggttacg atttagcatt atctgaattt ggtgatcaag tattcacttg
     3961 gcaacaatat gacgaaattg ttgaaaatga aggcagagat gctgctaatg ctgctcaaga
     4021 aaaagctgaa aaagaaggca agattatcat taaagattct attgctgaca ttttcttaca
     4081 acaaatttta actcgtccag ctgagcatga tgttgtagca actatgaact tgaatggtga
     4141 ctatatttca gatgctttag ctgcacaagt tggtggtatt ggtattgcgc caggtgcaaa
     4201 cattaattat gaaacaggtc atgctatttt tgaagcaaca catggtacag ctccaaaata
     4261 tgcaggttta aataaagtga atccatcttc agtaatttta agttctgtat taatgttaga
     4321 acatttagga tggcaagaag cggcagataa gattacagat tcaattgaag atacaattgc
     4381 ttcaaaagtt gttacttatg actttgcccg tttaatggat ggtgctgaag aagtttctac
     4441 atcagcattt gcagatgaat tgattaaaaa tttaaaataa gcaaaataaa attaggtgag
     4501 tctagcaact ttaattgcta ggttcgcctt tttaaatgaa ttgaagttgg caacaggata
     4561 gaaataataa agagtcacca atgattgatt atgtagtgtt tcttacacat tagacaaagc
     4621 gaatatgtac ataagcttag gaataaatga gtaaaactca tgcataggta gtgggataga
     4681 aatgctattt ttacaaaatt tatttcgttg tcccagagta agtcatgcaa atgatagaag
     4741 gaaatataag aagtatttta tttatatgta aagtaatgta aaaaatgtgt aaagataatg
     4801 tgtaggatgt tatttttata aatagttcgg ttataatgta aatataatta taagttaggg
     4861 aggcatacca tgtcgcaaaa agtgttggta gtagatgacg aacattcaat cgtaacatta
     4921 cttaaatata acttagaaac agctggttat gaagttgttg tcgcatttga tggtgatgag
     4981 gctttagaaa aggtagaaag tgaacagcca gatttaatta ttttagatgt tatgctacct
     5041 aaaaaagatg gcattgacgt atgtaagact gtaagaacaa ataaaaattt agtccctata
     5101 ttaatgttaa cggcgaaaaa tgatgaattt gatcgggtat taggtttaga attaggtgcc
     5161 gatgactata tgactaagcc tttttcacct agagaagttg ttgcccgtgt gaaagccatt
     5221 ttaagacgtt ctcaatttgt aaaagaagta gaagagattg acgatgatat taccatcgga
     5281 tcgatacgta ttagacccga ttattttgaa gtatataagc ataatgagtt attggagcta
     5341 acaccgaaag aatttgaact cttgctttat ttgatagaaa gacaaggcag agtcattacg
     5401 agagagcata tgttaaatac ggtatggaat tatgaatttg caggagactc aagaattgtt
     5461 gatgtacata tcagtcattt aagagataaa ttagaagata atcctaagaa gccacaactt
     5521 attaaaactg tgcgtggctt aggctataaa ttggaaagac ctaaagaaca atgatgaagt
     5581 ttcaccaccg cttaatgtta ttgataagca gtatattaat tatcagtttt gttacattag
     5641 gcattattat tcacgcttcc atcacatatg ttttatcaga aaaacacgaa caagctctag
     5701 caacagatgc aagatcatat atttatttag tccaagataa tgagattgat aaagttaaaa
     5761 aaattgtaaa acagcaaaat atagatttat tgataactaa gcatgataaa aaggtgttta
     5821 gtagtggcaa actgaaagct atcccaaata gtgatagcta tattcaaaaa agggacgttt
     5881 ttatttttaa taagaaggtt aacggttatc atgtatggat taaaggttac aataacgata
     5941 ttacagaaat gcattggacg ttgtggaaat atcttatttt gacatgttta gttgtactca
     6001 tttgtctgta ttttgcaagc cgtagtttta agcgtacatt aatccgtccc attcaagaag
     6061 ttacttatgc aacacaattg ttggcaaatg gttattatca catacgtgta cctgaaagta
     6121 atgttgtaga aactaaagcg ctttttgtgt ctaccaatga tttagcacga cgccttcaaa
     6181 agcttaataa tgagcaaaaa attcaatcca accgtttaaa aacaacgatt gaaaatattc
     6241 caagtgctat tttaatgata gatcgaaatg gaaaaatcgt tgttgcaaat aaagcgtatt
     6301 atgaacagtt caacatctcg cataatattg aacaagtagg ctatcacggc tatgttaata
     6361 ctgaaattga gcaattaatt ttagaaagtt ttaaagttga aaaacctatt tatgaacaat
     6421 tggaagttgc aataaaccaa gtccatgcca agtactttga tatttcatgc gttccgattt
     6481 taactaggtc tcaaaaaagt ttgcaaggga ttttagttgt gatgcatgat ataacaaatc
     6541 tgaaacaact tgaaaatcta agacgtgaat ttgtagcaaa tgtttcacat gaattaaaaa
     6601 cacctattac ttcaattaag gggtttgctg aaacgcttat tgatggtgca aaaaatgatg
     6661 cggaatcatt agacatgttt ttaaacataa ttttaaaaga atcaaatcga atagaatctt
     6721 tagtaacaga tctgttagat ttatcgcata ttgagcaaca tacagagtta gatacagact
     6781 atatgaattt atcagattta acgcggcgta ttattgataa tatgatgact caagccaatc
     6841 aaaaaaatat ttccattcat actgatattg aaaaagatgt cattgttaaa gcacaagaaa
     6901 gtaaaattgc tcaagttatc acgaatttgt taacgaatgc aattaactat tcttatgaag
     6961 atggagatat taacgttcgt gtgtatcgag atgactttcg tgtcattttc gaagtacaag
     7021 attttggtat aggtattaaa ttagaagacc aacaacgtat ttttgaaaga ttttatagag
     7081 tagataaagc acgaagtaga gattctggtg ggacaggtct tggattatca attacaaagc
     7141 atatagtaga ggcccaccaa ggcaatattg aagtgaatag tcaagttggc aaaggctcaa
     7201 cgttcaaagt tattctaaaa gattataaag aataaaaatt aaagtggtaa cagcgcgtgt
     7261 atttttcacg aagttgttgc tgctttttta tttcgttcaa atctctattg gtattaaatt
     7321 agagttaatg aagtggaata aattcggttg tcaattttgt catttgtata tgtcgatttg
     7381 taagttataa gta
//
LOCUS       DAMQYT010000129         7325 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMQYT010000129 DAMQYT010000000
VERSION     DAMQYT010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.129"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(196..1863)
                     /locus_tag="PGA33_001968"
     CDS             complement(196..1863)
                     /locus_tag="PGA33_001968"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDG6473772.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVEGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKVREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWKKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            complement(2303..4009)
                     /gene="acsA"
                     /locus_tag="PGA33_001969"
     CDS             complement(2303..4009)
                     /gene="acsA"
                     /locus_tag="PGA33_001969"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDG6473773.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRIFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            4178..4810
                     /locus_tag="PGA33_001970"
     CDS             4178..4810
                     /locus_tag="PGA33_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDG6473774.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTHEDLKALTFDAHLTAFRD
                     AEDQYKALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGKLPYLIELGAIE
                     VSINFRQLQLAEKLIQLSLSTPEFENYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            4835..6004
                     /locus_tag="PGA33_001971"
     CDS             4835..6004
                     /locus_tag="PGA33_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HDG6473775.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(6102..7091)
                     /gene="ccpA"
                     /locus_tag="PGA33_001972"
     CDS             complement(6102..7091)
                     /gene="ccpA"
                     /locus_tag="PGA33_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDG6473776.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(7164..7277)
                     /locus_tag="PGA33_001973"
     CDS             complement(7164..7277)
                     /locus_tag="PGA33_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473777.1"
                     /translation="MKKVYIKLKYIVRYHLAFVFISSFSLNFSKQKYVTVN"
BASE COUNT         2280 a         1437 c         1047 g         2561 t
ORIGIN      
        1 tcgctttcct attcttcaag cccacttttt atcttcatat acattaatta accaccttca
       61 aacattgtcg ttagattcgc caaattgaat ctatttttag cacagcaaaa aaccgaactg
      121 ctgaaataat gcatctccaa cagtccggtc tattaaacta tttacttatc tttattaaac
      181 aattgacatg atttattaga ataacccaat tgcatgacca tcatcagtaa catccatgtt
      241 taatgctgct ggttttttag gtaaaccagg catagtcatg attgcacctg tcaacgctac
      301 gataaatcct gcacctgttt tcgcttctaa ttcacgaatt gtaatttcaa atcctgatgg
      361 tgcacctaac aacgtttgat catctgagaa tgaatattgt gttttcgcca tacatactgg
      421 gtaattatcc caaccatttt ctttaaattg ttttaattgt ttttgcgctt tactgctaaa
      481 cgttactttt gaaccgccat agatttcagt cacaatcttt tcaatctttt gctctaatgg
      541 taattctaat tcatataaag gtttaaatga attaggttga tcaatgactt ctaatacttc
      601 atttgctaag tcaacgccac ctttaccacc ttttttccaa acttcagtta aggcaattcg
      661 tacgttattt tctttagccc aagattttac atattctact tctgcatcgg tatcatgtat
      721 aaatgcatta attgcaacaa ccggttctac accgaatttt ttaatattat taacatgacg
      781 ctctaaatta acaattcctg cttttactgc ttctacattt tcttctttta aattatcttt
      841 cgctacacca ccatgcattt ttaacgcacg aattgtcgca acaacaacga cagctgccgg
      901 atcaaatcct gcttcacgca ctttaatgtc catgaatttt tcagcgccta agtctgaacc
      961 aaatccagct tccgttacaa cgatatcagc taaatcacgt gctgtttcag ttgctaaaat
     1021 tgagttacaa ccgtgtgcga tattcgcaaa tggtccacca tgaactaatg caggtgtccc
     1081 ttcaattgat tgtactaagt ttggttttat tgcatctttt aaaatcattg caagtgcacc
     1141 ttccactttt aaatctgcaa ctgtaactgg cttgcgatct ctagtgtaac caatagtaat
     1201 acgactaatt ttatctttta agtctttaat acttctactt aaacataaaa tcgccataat
     1261 ttcagacgct actgtaatat taaagccatc ttcacgtggt acaccatttg taggtccacc
     1321 taacccaacg tttacatgtc taagtgcacg atcattcata tctaatacac gtttccactc
     1381 aatacgtctt tgatcgattc ctaattcgtt accttggtga atatgattat cgataaacgc
     1441 agacaatgca ttatttgcag ttgtaatcgc atggaaatct ccgttgaaat gtaagttgat
     1501 atcttccata ggtaagactt gcgcataacc accaccagtc gcaccacctt tgataccaaa
     1561 tgttggtcct aaagcaggct ctcttaatgc aaccataacg tttttattta actcatggaa
     1621 tgcatcagct aaaccaactg taaccgttga tttaccttca ccagctggtg ttgggctcat
     1681 cgcagttact aaaacaactt tccctttgtt ttctcttggc gtaattttat taatgtcgat
     1741 tttagctttg taatgaccat aaggttctaa tgcatcctct gaaataccta ctgatgcagc
     1801 aatatcctta attggttgta gtgttgattg attcgcaata tctaaatctg ataaatgagt
     1861 caactttttt cgcccctttt tgtttaattt atgcatttat aatatgtaag ttgtagctaa
     1921 gaaattttta cttacattca tataataacg aactgttaaa aacttgtcga ctaaatcggg
     1981 ttagcgcttt catttttgca ataaattcac ttattaaagt tttgtattat acaactttta
     2041 gcgcttgata ctattgataa ctatattttt ctattattta aaataatcat cttccaaaga
     2101 tcatattaaa aataaaaagc aatataaaga tatgccatta ttgttatgtc atcattttca
     2161 aatgctttct acgaaatgat atgacacaag ttaacagttc tttatattgc ttatcgtaaa
     2221 gtaaacgatt atttaaacat tgacatgaaa tatgttgtca ccctttattc ttcgaaaaaa
     2281 atcttcaata acattcatgt cattattcca ttgtacttaa atccccagca tctaaattta
     2341 attcccaagc ttttaataca cgtctcataa ttttacctga ccgtgtttta ggtaatttat
     2401 ctttaaattc gatttcacgt ggtgctgcat gtgccgacaa accttcttta acaaatatac
     2461 gaatttcttc ttttaattcg tctgttggtt catatccttt tctcagtgca acaaacgcct
     2521 taattatttc accgcgaacc ggatcaggtt taccaataat tcctgcttcg gcaactgctt
     2581 cgtgttcaac caatttagac tcaacctcaa atggtccaac tcgttcacca gctgtcataa
     2641 ttacatcatc aacacgtcct tggaaccaga agtaaccatc ttcatcttta tatgccgaat
     2701 caccagatac ataccagtct ccaataaaat atgatttata tttttctgga ttcttccaga
     2761 tacgatacat cattgatggc cagccttttt ttatagcaag gttgcccatt cgatttggtg
     2821 gtaattcatt ccctgcatca tcgataattg cagcttgaat accaggtaat ggtttgccca
     2881 ttgagccaag cttgacgtcc atcgttggat agttaacaat catatgtcca cctgtttctg
     2941 tcatccacca agtatctaac accgttaaac cgtatacttt tttcgcccat tttataactt
     3001 caggatttaa aggctcacct actgatagaa tcgaacgtaa cgatgacaag tcatatttct
     3061 caacaatatc gtcaccagca ctcattaaca ttcttaaagc tgttggtgcc gtataccaaa
     3121 tcgtcacttt aaaatcttca atcatactat accactgttc tggcgaaaag cgaccaccag
     3181 ctatacaatt tgtagcgcca tttaaccatg gtgcaaaaat accataagat gttcctgtaa
     3241 cccaacctgg atctgctgta caccaataaa catcatcttc ttgtaaatct aatacatatt
     3301 ttccagaaat atagtgcact aacattgctt gttgaacatg caatacacct ttaggttgcc
     3361 cagtagaacc tgatgtataa tgtaaaatca aaccatcatc cgactttaac cattcaatgt
     3421 caaattcatc gctagcagtt tccatcaaac taatgaagtc tatgtaattg tcttctacat
     3481 cctcatctac gacaacaatt tttttcaagt ttggtaattt atctacaggt actcgaggta
     3541 acaatgcctt attagtaatt aacactttag cttcactgtt ctctaatcta tccgcaactg
     3601 ccttttccat aaatgcttca aataacggcc caacaattgc accaattttt aaaacaccta
     3661 acaacgcaaa atatagttca ggtgtacgcg acataaatat aaatactctg tcacctttgt
     3721 caacttctgc atgttcagac aaaacattcg ctgctttatt agataaccgt tgcatatctt
     3781 tataagtata cgattctttt ctgtgctcat ctttgtaatt taacgctatt ttatccccta
     3841 atccttgatc tacatggcga tctatgcatt catatgccat gttcattttt ccagtttcac
     3901 tccaagaaaa tgcttgttct acgtctttcc aatcaaaagt attatatgtt tcttcataat
     3961 ctttaaggtt atgtttacct tgcgctcctt tataaacttc gactttcatt tgaaactccc
     4021 cctttgttat gtgaaaacgc tttctttctc aatattatac ataacatttc taattattca
     4081 aaatttttac ttttatcctt tttctagtat taattattgt aaattttcta tataatgaat
     4141 ttaatacaat cacgacgtcg attgaggagt tgaaaatatg aatcatttaa agacgtatca
     4201 atccgaagat tattacattc atgacaagca atttgttatt gaaggtcctt taacacacga
     4261 agatttgaaa gcgcttactt tcgatgcgca tttaaccgca tttagagatg ctgaagatca
     4321 gtataaagct ttgttagaaa ttacaacatt accagaaggt agaatttatg ttgctcgcca
     4381 agatcaactc attgtgggtt atgtcacttt ccactatcct gatgaaattg agcgctggtc
     4441 tacaggtaag cttccatatt taatcgaatt gggggcaatt gaagtcagca tcaattttag
     4501 gcaattacaa cttgcagaaa agctgataca acttagcctt tctacaccag aattcgagaa
     4561 ttatatcgtt ataactacag aatattactg gcattgggat ttaaaaaatt caaagttaga
     4621 tgtatttgac tataaaaaat taatgcagcg gttaatggca actggtggac ttgaaatatt
     4681 cgctacagat gatccagaaa taacaagtca tccagctaat tgtttaatgg caagaattgg
     4741 caaaaacatt acattagaac agcaacaagc gtttgatgat attcgttata tgaatcggtt
     4801 tttcttttaa tttttggaat atcggaggga ttatatgcaa caacattcat caaaaactgc
     4861 atatgtttat tcagataagt tattacaata tcgatttcat gaccaacatc ccttcaatca
     4921 aatgcgttta aaattaacaa cagagctact tttgaatgca aatttattgt ctccagaaca
     4981 aatagtacaa cctagaattg caacagatga cgaattaatg ttaattcata aatatgatta
     5041 cgtcgaagct attaagcatg cttcacatgg cattatcagt gaagatgagg ctaagaaata
     5101 tggattaaat gatgaagaga atggtcaatt taagcatatg caccgccata gtgccacaat
     5161 tgttggaggc gctttaactt tagcagatct tattatgtca ggcaaagtat taaatggttg
     5221 tcacttaggt ggtggtttgc atcacgctca acctggtcga gcaagtggtt tttgtatata
     5281 caatgatatt gcaattaccg cacaatactt agctaaagaa tacaatcaac gcgttttaat
     5341 catagatacc gatgcacatc atggagatgg tacacaatgg agtttctatg ccgataacca
     5401 tgttactact tattctatcc atgaaaccgg aaaatttctt ttcccaggct ctggtcacta
     5461 tactgagcgc ggtgaagata tcggctatgg acacactgta aatgtcccac ttgaaccgta
     5521 tacagaagat gcatcatttt tggagtgttt taaattaaca gttgagcctg tcgtaaagag
     5581 ttttaaacct gatattattc taagcgtaaa tggtgtcgat atacattatc gtgatccact
     5641 aactcatcta aattgtacgt tacattcatt atatgaaatt ccatattttg taaaatattt
     5701 agctgattct tatacgaatg gaaaggtaat tatgtttggt ggcggaggct acaatatttg
     5761 gagagtcgta ccacgtgcat ggagtcatgt attcttaagt ttaattgatc aaccaattca
     5821 aagtggttat ttaccgttag aatggattaa taaatggaaa cattattcat ctgaattatt
     5881 acctaaaaga tgggaagatc gtttaaatga ttatacctat gtcccccgca caaaagaaat
     5941 tagtgaaaaa aataaaaaat tagctttaca tatagcgagt tggtacgaat ctactcgtca
     6001 ataagtaacc taatctgcaa cgattttaaa agataaataa aaggcattct tccaacaccc
     6061 atataatttg ggtcgttaga tgaatgccta attttgtgaa tttattttgt agttcctcgg
     6121 tattcaattc tgtgaggtaa aactacattt ggttcttcta tcttttcatc gttcatatat
     6181 tttgttaata agcgcatccc tactgcaccg atatcatata atggttgaat aacactagaa
     6241 agttgtggtc taaccatctc aactaatcgt gtattattga aactaataat ttgtaattcc
     6301 tctggaactt taataccagc atccattgca ctatgcataa taccaattgc ttcttcgtcg
     6361 ctgatacata aaatggcatc tggcaaattg cctttcattt tggcaaaagc ttttacgcct
     6421 tctttataac tttcagcacc agaacaattc aatgtatcac ctaattgaag gccattttta
     6481 tttaacactt cagttaaacc ttctaaaaca tcttcttgag cttttttaga atgttctcca
     6541 cctactaaag caaatgattt agcgcctttt tcaattaatt ctcccgtaat ttctttcgca
     6601 gcttcagtaa aatcaatatt aactgatgct atatgtgcat ccttaccatt tgttcctgat
     6661 actactacag gtacagatga ttgatttatc aattctttca tttcttcagt aattgtacca
     6721 ccaaggaaaa taataccatc aacctgttta cttaataagt tattaaaaat ttctttttcc
     6781 ttttcaggat cgttatctga atttgaaata attgagtgat atttatacat tgttgcaata
     6841 tcttcaagtc cacgagcaag ttgtgaataa tagatattag atatatctgg aatgatcaca
     6901 cctactgttg ttgtcttttt actagctaaa cctctagcaa cagcatttgg acgataattc
     6961 aaacgcttaa tgacttcgtt aactttattt ttagtttctg ctttaacatt ttggttccca
     7021 ttaacaacac gcgacactgt ggccatagag acacgcgctt ctcttgctac atcatatata
     7081 gtaactgtca taatttcctc cttgtaaacg ttttattcat tataataaca tgttttcatt
     7141 tcattttcat agtttaccat attttaattt acagtaacat atttttgttt ggaaaaattt
     7201 agcgaaaagc ttgaaataaa aacaaacgct aaatgataac gaacgatata ttttaatttt
     7261 atataaactt ttttcatttt taattgtaat gttttgagaa agccttttca aatatcaatt
     7321 gcatt
//
LOCUS       DAMQYT010000130         7306 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMQYT010000130 DAMQYT010000000
VERSION     DAMQYT010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.130"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..1030)
                     /gene="ribB"
                     /locus_tag="PGA33_001974"
     CDS             complement(<1..1030)
                     /gene="ribB"
                     /locus_tag="PGA33_001974"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDG6473778.1"
                     /translation="MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEH"
     gene            complement(1041..1673)
                     /locus_tag="PGA33_001975"
     CDS             complement(1041..1673)
                     /locus_tag="PGA33_001975"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDG6473779.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            complement(1680..2723)
                     /gene="ribD"
                     /locus_tag="PGA33_001976"
     CDS             complement(1680..2723)
                     /gene="ribD"
                     /locus_tag="PGA33_001976"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDG6473780.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     regulatory      complement(2850..2985)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(3204..4706)
                     /locus_tag="PGA33_001977"
     CDS             complement(3204..4706)
                     /locus_tag="PGA33_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDG6473781.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSGQSWQAYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECADYDI
                     DFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDI
                     YNVVINATGAKSHLNELDQDDQLIRNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSDNQ"
     gene            5229..5543
                     /locus_tag="PGA33_001978"
     CDS             5229..5543
                     /locus_tag="PGA33_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDG6473782.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFSQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            5543..6835
                     /gene="arsB"
                     /locus_tag="PGA33_001979"
     CDS             5543..6835
                     /gene="arsB"
                     /locus_tag="PGA33_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HDG6473783.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHVGFVRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF"
     gene            6853..7248
                     /gene="arsC"
                     /locus_tag="PGA33_001980"
     CDS             6853..7248
                     /gene="arsC"
                     /locus_tag="PGA33_001980"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HDG6473784.1"
                     /translation="MTKKTIYFICTGNSCRSQMAEGWAKQILADDWNVYSAGIETHGV
                     NPKAIEAMKEVGIDISNHTSDLIDNNIIKNSNLVVTLCSDADVNCPSLPTNVKKEHWG
                     FDDPAGKPWSEFQRVRDEIKIAIENFKSR"
BASE COUNT         2290 a         1289 c         1073 g         2654 t
ORIGIN      
        1 tatgttcgat gttaaaatat tttaaaatct gtgcagcaat atgataatct cgcaaatctt
       61 catcaaaacc taatgctaaa tttgcagtta ctgtatcata tccttgctca attaattcat
      121 atgcgcgtaa tttgtttaac aatcctatgc cacgaccttc ttgaggtaga taaataatca
      181 tgccaccatg ttcattgata tacttcatag acgattcaag ttgagcacca caatcacaac
      241 gttgactatg gaaaatatcg cctgtaaggc acgcagaatg taagcgtaca ttttcatgtt
      301 gtcgaattgc accttttgtc agtacaacta tctcttcatc tgtgtatgtc gctttaaaac
      361 catacatatc aaatgttccg aaatctgtag gcattttcac ttttgcctta aattcaattt
      421 ctggttctaa ttttttacga tattcaatta aatcatcaat cgtaatcatc tttaattgat
      481 gtttttcttt aaacttttgt aaatcttgtc ctttcgccat cgtgccgtca tcattcataa
      541 tctcacaaat gacaccagcg ggcttggcac cagtaagttt agctaaatca acagccgctt
      601 ctgtgtgtcc atttctagct aatacgcctt tatcttgtgc tactaatgga aataaatgac
      661 caggacgatt aaaatcttta gcttcactac taggatcaat gagctttttg gcagtcaatg
      721 tacgttcata agcactaatt cctgttgttg tatctacatg atcaatactc actgtaaatt
      781 gcgtaccaaa gatgtcggag ttatcatcaa ccatttgtac caaatccaaa cgttgtgcaa
      841 tatctttaga cactggtgcg catattaatc cccttgcttc tttcgccata aaattaatgg
      901 tattatcgtt catccattca gtaaccgcta ctaaatcacc ttcattttca cgattctcat
      961 catctactac aataattggt tctccatttt ttaaagccat taaagcactg tcaatattat
     1021 cgaattgcat gctacccctc ctaaaaacca aatgctctta atttatctac agataattgg
     1081 tctttatctt tatttaaaat attttcaaca tatttaaaca aaacgtctgt ttctaaatgt
     1141 actttatctc ctaatttttt ggatgataaa atcgttgaac gcctcgtttc tggaataaga
     1201 tgaatgtcaa aactgttatc atgcttatca aataccgtta gactaacacc atccacagta
     1261 atagaccctt gcttaactaa ctgattatta atatgttggc tacattgaat cgtaataatt
     1321 tttgcattgg ctgtttcatt tatttttgaa actgttccta gttcatctac atgaccgagg
     1381 acaaaatgtc caccaaacct accgttacca ctcatggcac gctctaaatt tacttctgat
     1441 tgtcgcttaa catctgctaa ataggtttta ttttcagtgc ctttaattac ttgaacagta
     1501 aaagatgtct gattaaaatc aatcactgtt aaacatgcac cattaacact gatggaatca
     1561 ccaatatgca tatctgccgt aatcttatgt gcttcaattt caatcgtcct gactgattga
     1621 cgaatttgaa cacttttaac gacacctatt tcttcaacga tgccagtaaa catgcatcat
     1681 cacttctttc gtaaagttaa tttaacattt tgatttaata actcggaatg aacaatttca
     1741 aattggttcg catctggtat ctcaatcaca tcatttgttt gataaaattg ataatttcca
     1801 gatccgccaa ttaatttcgg ggcataatag agaataaatt catctatata attagattgg
     1861 agaaattctg aagtagtggt tggacctgcc tcaactagca aagttccaac tcctctttta
     1921 tataaattgt gaagaattgt tgttaaatcg caagacttca agtaaataat ttcaatatgt
     1981 gtttgattgg ttgttaaatt tggattttca gtatatatcc aaattggtgt tgattcatct
     2041 tgataaattt gctgattaaa atgaatattc ccagacttag acaatattac ttttataggg
     2101 ttttttccat cttgaatacg tgtagtatat tgtggatcat ctaattcaac tgtacgtctt
     2161 ccagttaaca ctgcgtcgtg tcgatgtctt aacttataga catcttgttt aacctctttg
     2221 ttagtaatcc attgactttg tccattatca ttcgcttgtt taccatctaa acttgcagat
     2281 actttcactg taatttgtgg cagttgcttt gcttttgctt taaaaaagtc ttggtataat
     2341 tgtgatgccc gttcatcatc aacgcattca acctcaatac cgtgagcccg taacgtctca
     2401 tcaccatgtg tgtctaacga attgtctttt gttgcgtata ctacttttgc tatcttacaa
     2461 tcaattattt tgttaacaca gggtggtgtt gaaccaaaat gactacatgg ctctaacgta
     2521 atataaatcg tcgcaccttc agcattttgt tgtgccatat caagtgcttg aacctccgca
     2581 tgcttgtcac cttttctcaa gtgtgcacca ataccaacaa tcctaccttc tttaactaca
     2641 acagcgccaa cgggtggatt aacacctgtt tgaccttgta ccatatttgc aagttgaatc
     2701 gcataatcca taaattgact caaatgatca cctctataaa caaaaatcct cacatcatga
     2761 attaagatgc aaggagaaaa atttatcgtt aaataagcct atttgtacac atttttacaa
     2821 atacgctaca ttatctttgt cgataattaa cattctttct cccatccaga ctttaactgt
     2881 cggctctaga atctcactag atcagccact aatatgaaac atattagcag gtcgcaggct
     2941 ttatttactg ccggttggga atttcaccct gccccgaaag aattatatat gaaattgtta
     3001 tagattattt gagtacgtag tatgtcaact acatttaaaa tgatactata tgttttctga
     3061 aaaaacaatt aatgacggtt ttaatttaat ataatctgag tactataggc atctcattga
     3121 tatgattctt actaacagac attaaaatca aaccttcaat tcgtctctat agagcgttct
     3181 ctttattatc ttctagttac aaattattga ttgtcactgc gctgttgttg ctcattcgat
     3241 tctaaagcat catataattg agatactgta tgcgcaactt gttctacaat cattttcaca
     3301 ccgtttcgta gtttattaac accgtttgtc atttgaccta tcgcaatcat atttgttaat
     3361 gttccaaacc ttggactaat aacttgattg gtttccggaa tgatttgtat gcctcccatt
     3421 gggtgtgctt gtacaatttg tctattttca agatttctaa ttaattgatc atcttgatcc
     3481 aattcattta aatgactttt tgcacctgtc gcgttaatga caacattata tatgtctact
     3541 gattcttggt ttttgtatga aaaataatac aacttgccat catgtttcac atcttctaaa
     3601 tcttttttca aaattaaaga cttattttct attaattcaa taattagttc agcagttctt
     3661 ggaggcattg gatttgaatt taattgaatc atctttgagt atttttgatt aaattgatgt
     3721 tggtcttcaa tacttaagct attccatatc caatttaaat tctctttcaa atgttcaatc
     3781 atactttgga aaatgcccat ttctgttgga cgcgctaaat catacttcaa atctgcaata
     3841 tgatttcctg tacgtctatg tactaatttt ttaaaatcaa tgtcataatc agcacattct
     3901 tttaaaaata aagaaactaa agtatcaagc ggtgcattgc cgaaatgatg ttttttaatg
     3961 tcatttaatt tgtctttagt taagtacttg aatgtcacgt ctatcattgt acctcttaca
     4021 cttggtaaat gagcagaacg actcgtcata gtaattggta attttggatg atgagcagca
     4081 acataacgga caacatctaa actggcaagg cctgtaccaa taatcgcaat atcgtccagt
     4141 tcatttactt cgtctaacgt attatatgtt ggataaggcg tagcgatata tcctttttta
     4201 ccctttaagt tatatggatc atggtaggca aatgtaccac atgttaaaaa tacataatcg
     4261 tacgcttgcc atgattgtcc tgaatttgta gtacatatgt aataagttaa attcgtttca
     4321 tcgatattag aatttgtata aatctcttga actttattat aattagttga tatatttgga
     4381 tattttttcg tgaacataga taaataagat ttcatataat gtccgaatac aaatctcggt
     4441 aaatatgcag gttcatcaaa attaaaatca gtttgttgtt tataccactt ccaaaattca
     4501 gtctcatcat ctaaatttaa actcatcttt ttcgaaggca tattaattag cagctcagaa
     4561 ctatcatttt gaaatggtac gccctgtccc atatttactt tatcatcgta taaatctata
     4621 tctaattgat taaacttcgg gtgcttaact aactctctca atacacttac accagcagtt
     4681 cccatgccta ttattgctac acgcatatat catccatcct tttgtaattc aaaatattat
     4741 atttaaacta ttacctataa ttatagcaat ttggtatttc caataaaatt tattaaggta
     4801 agctattatt tcactttcac aaccattaaa ccatttaaaa cttttcattt aatatcaacc
     4861 ttagtgtttg tgttaaaata tttttaacga ggtgaattaa attgaaattt atagcaaaga
     4921 tattaatctc attagtaatt ctatttatga tttttagtcg taaaaacaaa gcttcttaat
     4981 caataccctt gattacaaaa taaattcaac taaggtgatt ggccatacac tcaagatatg
     5041 gctaatcacc tttttcaatg cagaacaaag agtatatttt gcaaaatcaa tatctataat
     5101 ttatctttaa taccctttta atactaaaac caaatttaaa ttacatagtc atatcgtgta
     5161 atataccaaa ggaacataga cattcatcta tatagatgat aatctattta acgtctagga
     5221 ggtatgttat gacgtataaa gaactagcaa catttttaaa agttttatca gattcaagca
     5281 gattagaaat actagattta ctttcttgtg gagagttatg cgcttgtgat ttgttagcac
     5341 attttcaatt ctctcaacct acacttagct atcatatgaa agcattagta aaaaccaact
     5401 tagttacgac acgaaaaatc ggaaataaac atttatacca gctcaatcat aatatttttg
     5461 agtccgtaat taataacttg tctaaggttc atacctctaa ccaacgatgt atttgtcata
     5521 accttaagac tggtgaatgc taatgatgac aactttagcg acactcattt ttctagtaac
     5581 tttattattt gtattatggc aacctaaagg cctagatatt ggcattaccg cactaactgg
     5641 tgcctttatt gctgttatta ctggtgttgt aagtttttcc gatgttttcg aagtaacagg
     5701 tattgtttgg aatgctactt tgacttttgt ctcagtcatt cttatttcat taatattaga
     5761 taaagttgga ttattcgaat ggtcagctat tcacatgctt catgcttcaa aaggcaatgg
     5821 tttaaaaatg ttcgtttata tcatattatt gggtgccatt gttgctgcat ttttcgcaaa
     5881 tgatggcgca gcgttaatct taacgcctat tgtattagcg atggttaaaa atataggttt
     5941 tagtaagcgg gccatattcc cttttattat tgcgagtggt tttatagctg acacaacttc
     6001 tttaccttta atcgtgagca atctagtgaa tattatatct gctgattatt ttcatgtagg
     6061 attcgttcga tattttagta gaatgattat acctaattta ttctcgcttt tagcaagtat
     6121 tatagtattg tggttatatt ttagaaaagc gatacctaaa acgtttgatg ataataatat
     6181 aaagcatcct aaagatgcca ttaatgattt aaagctattt aaaatttcat ggattgttct
     6241 agttatatta cttttcggct atctaatcag tgaatttact aaaattccgg tatcaatttt
     6301 cactggaatc attgctttta tttttctaat gttggctcgt aaatcaaatg ctgtaaatat
     6361 taagcaagtc attaagggcg caccttggaa tatagtatta ttttcaattg gtatgtatat
     6421 cgtcgtattc ggcttaagaa atgctggcat tactttaata ttggctaaaa tattagaata
     6481 tatttccaat tacggtctat ttagcactat tttgggaatg ggcttcattt cagcgttttt
     6541 atcatcaata atgaataata tgcctacagt tttaatagat gcgattgcta ttggtcaatc
     6601 aaatgtccat ggcatgttaa aagaaggcct aatttatgcg aatgttatcg gttctgattt
     6661 aggtccaaaa attacaccga taggctcttt agctacatta ctgtggttac acgtcttaac
     6721 acaaaaagat gttaagattt cttggggcac atactttaaa actggtatca tcattacaat
     6781 tccagtacta tttataaccc tcatagggtt gtatctaaca cttatcatat tttaattaat
     6841 taggagattg ttatgactaa aaaaacaatt tattttatat gtacaggcaa ctcatgtcga
     6901 agtcaaatgg ctgaaggttg ggctaaacaa atcttagcgg atgattggaa tgtatattct
     6961 gctggtatcg aaacacacgg tgttaatccc aaagcgatag aagctatgaa agaagtaggc
     7021 attgatatat caaatcatac atcagattta atcgataata atattattaa aaattcaaat
     7081 ttagttgtta cattatgtag tgatgcagac gtaaattgcc cttctttacc aacaaatgtt
     7141 aagaaagaac attggggatt tgatgatcct gcaggcaagc cttggtcaga gttccaacgt
     7201 gtaagagatg aaattaaaat cgcaattgaa aatttcaaat cacgatgaga gtgggacaga
     7261 aatgataaaa aaacactaat gatttattat gtagtggttc tttatc
//
LOCUS       DAMQYT010000131         7121 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMQYT010000131 DAMQYT010000000
VERSION     DAMQYT010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.131"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            30..893
                     /gene="rpoD"
                     /locus_tag="PGA33_001981"
     CDS             30..893
                     /gene="rpoD"
                     /locus_tag="PGA33_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDG6473785.1"
                     /translation="MNPSDLSAPPGVKINDPVRMYLKEIGRVNLLSAQEEIELAKRIE
                     QGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNMGLIKAVEKFDFNKGFKF
                     STYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGRDPAPEEIGE
                     EMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQEAQSPSDHAAYELLKEQ
                     LEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHP
                     SRSKRLKDFMD"
     gene            1024..1701
                     /locus_tag="PGA33_001982"
     CDS             1024..1701
                     /locus_tag="PGA33_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDG6473786.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     ELIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            1704..2804
                     /locus_tag="PGA33_001983"
     CDS             1704..2804
                     /locus_tag="PGA33_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDG6473787.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDAYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKALLMNWFNTEKINLDVEASTINTDPFQYI"
     gene            2918..4264
                     /locus_tag="PGA33_001984"
     CDS             2918..4264
                     /locus_tag="PGA33_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDG6473788.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFYLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            4274..5164
                     /locus_tag="PGA33_001985"
     CDS             4274..5164
                     /locus_tag="PGA33_001985"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDG6473789.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            5290..6075
                     /locus_tag="PGA33_001986"
     CDS             5290..6075
                     /locus_tag="PGA33_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDG6473790.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            6117..6980
                     /locus_tag="PGA33_001987"
     CDS             6117..6980
                     /locus_tag="PGA33_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDG6473791.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            6967..>7121
                     /locus_tag="PGA33_001988"
     CDS             6967..>7121
                     /locus_tag="PGA33_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDG6473792.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGIS"
BASE COUNT         2615 a          931 c         1341 g         2234 t
ORIGIN      
        1 aaaaagatag ttcagatact gacgagaaac tgaatccaag tgatcttagt gcccctccag
       61 gtgttaaaat aaatgaccca gttcgtatgt accttaaaga aattgggcgt gttaacttat
      121 taagtgcaca agaagaaatc gaattagcca aacgtattga acaaggtgat gaagtagcaa
      181 aatcaagact tgcagaagcg aacttacgtt tagttgtaag tattgctaaa agatacgtag
      241 gtcgtggtat gttattcctt gatttaatcc aagaaggtaa tatgggtctt attaaagctg
      301 ttgaaaaatt tgactttaac aaaggattta agttttcaac atatgcaaca tggtggatta
      361 gacaagcaat cactcgtgca attgctgacc aagcacgtac gattcgtatc cctgtgcata
      421 tggtagaaac aattaataaa ttaattcgtg ttcaacgtca attattacag gacttaggtc
      481 gagatccagc accagaagaa attggtgaag aaatggattt accagcagaa aaagttcgtg
      541 aaattttaaa aattgcgcaa gaacctgttt cattagaaac accaattggt gaagaagatg
      601 atagtcattt aggagacttt attgaggatc aggaagcaca aagtccttca gatcatgctg
      661 cttatgaatt attaaaagag caattagaag atgtgcttga tacattaact gatagagaag
      721 aaaatgtatt acgattaaga tttggtcttg atgacggcag aacaagaaca cttgaagaag
      781 ttggtaaagt tttcggtgtt acacgtgaac gtattcgaca aattgaagca aaagcactta
      841 gaaaattaag acatccaagt cgtagtaaac gtttgaaaga ctttatggat taatttatat
      901 caattttaat gattgatacc aagacatgaa taagcgctta tttattttta gattaagatt
      961 taacttgagt gaggttggta cattgaataa tgttcaacct cttttttcat aaaggagaat
     1021 aaaatgattt cgttaaataa ccgattaacg acggtaagtc gatttttaaa acaaggtaca
     1081 atcgcggata ttggctcaga tcatgcttat ttgccaatct atgcaattca aaaccattta
     1141 tgcgaatgcg gtattgctgg agaagttatt caaggccctt ttcaagctgc tgtgaaaaat
     1201 gtagctgcaa accaattagt agatagaatt gatgtacgtt taggggatgg cttaagtgtt
     1261 attcaacctg aggacgtaat cgataatatc acaatttgtg gtatgggcgg accacttatt
     1321 gctaaaattt taaaagatgg gcaagataag ttaagtcaac atccaaggtt gattttacaa
     1381 agtaatatcc aaactgaaaa tttaagacaa acattacaac aattgaatta tgaaataatt
     1441 gatgaaataa taatggaaga aaaaggccat atttatgaaa ttgttgtagc agaatattct
     1501 acagaattaa tagagctatc atctgatgaa ttgaaattcg gaccaaaatt gctgaacaac
     1561 aaaaatgaat attttataaa aaaatggcaa agagagttag aagcacttta tcatattaaa
     1621 tcgaaactca atactgaaca acatcatcaa cgtttagctc aaattaatga cgaaattgct
     1681 gtaattgaaa gggtgttata gtaatgaaaa tagctgattt aatgacattg ttagatcatc
     1741 atgtgccatt tagtactgct gaatcttggg ataatgtagg attgttaata ggtgatgaag
     1801 atgttgaagt tactggtgtt ttaacagcat tagactgtac gttggaagta gtaaatgaag
     1861 caatcgaaaa aggttataat actattatta gtcatcatcc tctaatcttt aaaggcgtaa
     1921 catcattaaa agctaatggt tatggtttga tcattagaaa actaattcaa catgacatta
     1981 atttaatagc gatgcataca aatttagatg taaatccgta tggtgtcaat atgatgttgg
     2041 cgaaggcgat gggtttgaag aacatttcaa taataaataa tcaacaagat gtatactata
     2101 aagttcaaac atatatacct aaggataatg ttggaccatt taaagataag cttagtgaaa
     2161 atggattagc gcaagaaggt aattatgaat attgtttctt tgaaagtgaa ggaagagggc
     2221 aattcaaacc agttggtgaa gctaatccaa caataggaca aattgataaa attgaatatg
     2281 tagatgaagt taaaattgaa tttatgatag atgcatatca aaagtcaagg gctgagcaat
     2341 taattaaaca ataccatcca tatgaaacac cggtatttga ttttattgag ataaaacaaa
     2401 catcccttta tggacttggc gttatggcag aagtggataa tcaaatgaca ttggaagatt
     2461 tcgcagctga tattaaatct aaattaaata tcccaagtgt ccgttttgtt ggtgagtcta
     2521 atcagaaaat taaacgtatt gcaattattg gtggttcagg tattggatat gaatatcaag
     2581 ctgtccaaca aggcgcagat gtctttgtta caggtgatat taaacatcat gatgccttag
     2641 atgctaaaat tcatggtgtg aatttaattg atattaatca ttacagcgaa tatgtgatga
     2701 aagaaggttt aaaagcgtta ctaatgaatt ggttcaatac agaaaaaata aatttagatg
     2761 ttgaagcatc tacaattaat acagatccat ttcaatatat ttaaactaaa atctcgggat
     2821 ttctaaattt tgaaatttcg aggttttaat atttttattt aaaatagcac atttatactt
     2881 tataatagta aagatgaaca tataaggagg ccaaatcatg gcaaaacatc cattcgaaca
     2941 atttaatcta gaatctagtt taattgacgc tgtgaaagac cttaattttg aaaaaccaac
     3001 tgaaattcag aatcgaatta ttccaagaat actaaagaga acaaatttaa ttggtcaatc
     3061 tcaaacgggt acagggaaat ctcatgcatt tttattacca ttaatgcagt taattgatag
     3121 tgaaataaaa gaaccacaag caatcgtagt tgcaccaaca agagaacttg cacaacaact
     3181 atacgatgca gcgaaccatt taagccaatt taaagctggt gtttcagtta aagtttttat
     3241 tggtggtaca gatatagaga aagatagaca acgttgtaat gcacaaccac aattgattat
     3301 aggcacccct actagaatta atgacttagc taaaacggga catttacatg tgcacttagc
     3361 atcatattta gttattgatg aagcggatct tatgattgac ttaggattaa ttgaagatgt
     3421 agattacatt gctgcaagat tggaagataa tgcaaatatt gcggtgttta gtgctacaat
     3481 cccacaacag ttacaaccat ttttaaataa atatttaagt catccagaat atgtagctgt
     3541 cgacagtaaa aaacaaaata aaaagaacat cgaattctat ttaataccta ctaaaggtgc
     3601 agctaaagtt gaaaagactt taaatttaat tgatatacta aatccatact tatgtattat
     3661 tttctgtaat agtagagata atgcaaatga tttagcacgt tcactaaatg aagctggtat
     3721 taaagttggt atgattcatg gtggcttaac gccacgtgaa cgtaaacaac aaatgaaacg
     3781 tatacgtaat ttagaattcc aatacgttat tgccagcgat ttagcatctc gtggtattga
     3841 tattgaaggt gttagtcatg tcatcaattt tgatgtgcca aatgatattg acttctttac
     3901 gcatagagtc ggacgaactg gtcgtgggaa ttataaaggt gtagcaatta cgctttatag
     3961 tcctgatgaa gaacacaata tttcattaat agaagatcgc ggttttgtat tcaatactgt
     4021 tgatattaaa gatggtgagt taaaagaagt taaagcgcac aatcagcgtc aagcaagaat
     4081 gcgcaaagat gaccatttaa ctaatcaagt gaagaacaaa gttcgaagta aaattaaaaa
     4141 caaagttaaa ccaggttata agaagaaatt taaacaagaa gttgaaaaaa tgaaacgtca
     4201 agagcgtaag caatttagta agcagcaaaa tagacaaaaa cgtaagcaaa acaaaaaagg
     4261 ttaggtgtgt tttatgttat taggatcaca tgtttcaatg agtggtaaaa agatgttaga
     4321 aggttctgct atagaagcgc atgaatatgg tgaaacaaca tttatgattt atactggtgc
     4381 acctcaaaac acgcgccgta aaagtattga agatttaaat ataactaaag gtcatgaagt
     4441 gatggaaaaa tatggtttat ctaatattgt tgttcatgca ccatacatca ttaatattgc
     4501 aaatacaacc aaaccagaaa cgtttaatct tggtgtagat ttcttgcaac aagaaattga
     4561 aagaacgcaa gctatagggg ctaaagatat tgtattacat ccaggtgcgc atgttggagc
     4621 tggtgttgat gccggaatta ataagattat tgaaggatta aatgaagttt taacgaatga
     4681 caataatgta cgtattgcgc ttgaaacgat ggcaggtaaa ggtacagaga ttggtcgctc
     4741 atttgaagag ttagcacgta ttattgatgg agttcataat aacgaacgtt tatcagtatg
     4801 ttttgataca tgtcatacgc atgatgctgg atataatgtt aaagaggact ttgatggcgt
     4861 attaaatgaa tttgataaaa tcattggagt cgacagaatc aaagttgttc atgtcaatga
     4921 ttctaaaaat gaccgtggcg ctcaaaaaga ccgacatgaa aatattggct tcggttacat
     4981 tggttttgat gcattaaatt acattgtgca tcatgatagt tttaaagata ttccgaaaat
     5041 cttagaaaca ccttatgttg gtgaagataa gaaaaataaa aaaccgccat ataaacttga
     5101 aattgaaatg ttaaaacaac agcaatttga cccagaatta aaaaataagg ttatgcaaca
     5161 ataaaatgat aatgctctct tcaagaaatg cgataaataa aacttgaagg gggcatatct
     5221 ctttattttg tctaattttg agtcgtaaac attactgttt acatatgtta ctaggagttg
     5281 tatagttgaa tgcgaggtga gaatacgatg acaccagtct ttgaattgaa aaatgtcaat
     5341 tactactatg atcataaaaa agtgttagaa aatataaaca ttaaaataaa taaaggtgaa
     5401 tttttagcaa ttgttggacc aaatggtgct ggtaaatcaa cattattgaa gttgattcta
     5461 gggttattac ctttacaaag tggtgagatt tttgttgaag gtattgattt taaaaataag
     5521 aaaacatcaa ttaaattaag ctatgtatca caaaaagcaa atgcctttaa ttcaggtttc
     5581 ccagcaagtg ttaaagaagt tgttttaagc ggattaacaa agacaaaacg tcttttccaa
     5641 acatttaata gcaaagataa tgaaaaagtg attaaagtac tagaaagact gaatataagt
     5701 gatttaattc ataaaaatat agcagaatta tcaggtggtc aacaacaacg tgtaatgatt
     5761 gctcgagcat tgatttcaga acctgcagta ttagtacttg atgaaccaac gaatggtatt
     5821 gatgcaaaac atgtaagtga attttataat actttagatc aattaaaaca agaaggtatc
     5881 accattatct tagttactca tgatatcggt gttgtagcag atactgctac tgaagtagca
     5941 tgtttaaata agcatttgca tttccatggt acaactgatg agtttaaatc acttgatgaa
     6001 gttgaaattt caaaaattta tggacatcct gtacgttttg tcgatcatca gcataatcga
     6061 gaatgttgta attaataaag atttgagttt atttaaaatg gaaaggcgtg tcacaattga
     6121 tagatgcatt attgaatttt gattttatga ggtactcttt aattagtggt atcttgatag
     6181 gttttattgc gcctctaatc ggtgctttta tcgttgttag acgactatca cttatagctg
     6241 atgctctaag tcatgtaact ttaggtggta tatctttcgg tatgttttta cttactatta
     6301 tgccaacact agtatttatt aatccaatgt ggtttggaat cttattcgca atagtaggtg
     6361 cgcttctaat tgaaaaatta agaacgtcat acactgctta ccaagaaatt gctattccaa
     6421 ttataatgag tgctggtatc gccttgagtg caatcttcat ttcattagct gatgggttca
     6481 atcaagaaat tgttggattg ctatttggct caataagcgc agtgaatata agtgatttaa
     6541 caacgattat tgtcattgcc atcatagttg ttctgtttat tacattgttt tataaagaat
     6601 tatttatatt gtcatttgat gaggaatata gtaaagtcat tggcattcct aaatggattc
     6661 aatttttatt tattgttatt gtagcgatgg ttatttctgc ctcaatgcgt gttgtaggga
     6721 tactgttagt aagtgcgtta atcacgttac caattgcaat aagtatgcga ataacaaaaa
     6781 gttttaagca attgatatta ctaagtgtat ttttaggtga attatcagta atattaggct
     6841 tagtcctagc tttctatatg gacatttctc cgggtggtgt aattgtagtt ctacttgtta
     6901 ttttacttat gattacaatg gcttatcaga aaatgcgaat gaagtttaaa aagggagcta
     6961 atatcaatga atacaaatga tgctattaaa attttaaaag agaacggttt aaaatataca
     7021 gataaacgta aagatatgtt agatattttt gtcgaagaag ataagtatat aaacgcaaag
     7081 tatatacaac aagttatgga tgaaaattat cctggaattt c
//
LOCUS       DAMQYT010000132         7104 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMQYT010000132 DAMQYT010000000
VERSION     DAMQYT010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.132"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(371..817)
                     /gene="perR"
                     /locus_tag="PGA33_001989"
     CDS             complement(371..817)
                     /gene="perR"
                     /locus_tag="PGA33_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HDG6473793.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
     gene            complement(914..1864)
                     /locus_tag="PGA33_001990"
     CDS             complement(914..1864)
                     /locus_tag="PGA33_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDG6473794.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLRRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSKVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
     gene            complement(1870..2325)
                     /gene="bcp"
                     /locus_tag="PGA33_001991"
     CDS             complement(1870..2325)
                     /gene="bcp"
                     /locus_tag="PGA33_001991"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDG6473795.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            2406..3695
                     /locus_tag="PGA33_001992"
     CDS             2406..3695
                     /locus_tag="PGA33_001992"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDG6473796.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
     gene            3988..5082
                     /locus_tag="PGA33_001993"
     CDS             3988..5082
                     /locus_tag="PGA33_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDG6473797.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(5172..5264)
                     /locus_tag="PGA33_001994"
     CDS             complement(5172..5264)
                     /locus_tag="PGA33_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473798.1"
                     /translation="MFDFSTILKRKFVTGWWGHNIEKAVRDFLN"
     gene            complement(5273..7009)
                     /locus_tag="PGA33_001995"
     CDS             complement(5273..7009)
                     /locus_tag="PGA33_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HDG6473799.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
BASE COUNT         2340 a         1190 c         1029 g         2545 t
ORIGIN      
        1 aacaacactt atttttgttt aaaaattact gttgaaggga ttattttcta ttcgtaagta
       61 ctatatgtat ttaaagtgta attccttatg ctttttactt cagttttaca aacaaactta
      121 tactcacttt atggatagtt atttagtaga ccttcgtttg aatattttta tgtttattta
      181 taaaggatac tagttattag ttaataccac ttccgtagtt agaatattct tttcttcaat
      241 ttaaatattt ttcagaataa gcataaaaaa aagttaaaca actttcatgt cgtttaactt
      301 cttaaatatt acgcttactt atatgaagta agctaacgct tttttaattt gtcatactac
      361 caaagttaaa ttatttatct tggcattctt tacaaactcc ataaatttcc attcgatgat
      421 gtgttacgtc aaagtcagtc atatgctgag ctaatctttc aatttcattt aactgtggat
      481 attgaaaatc aacaatctta ccacattgtt cacatataat atgataatga ttatgtgtat
      541 taaagtcgaa tcgacttgat gagtctccat atgttaattc ttttacaatt ccaatatctt
      601 taaacactct taagttatta tatattgtcg caacacttat atttggaaaa tcaggtgaaa
      661 gtgcttgata aatttcatca gctgttggat gagtatgtga agaaattaaa taacgtaata
      721 ttgcttgtct ttgaggtgta attcttacgc ctgcttgtcg caatgatgca attgattctt
      781 ctagttcatg ttcaattgat tctatttcaa cactcatcta tatcaccatc tttcttatat
      841 aataattatt attacttact ataaatataa ctgtagtatt gcgcgcgtgt caatatttac
      901 atgatgattt catttaatag ccttttttag catcaacttc attctcaatt agaccattct
      961 tattgagaaa attaactaga ttgtttttaa aaatatctaa taagtcatac tttgcttcat
     1021 aatcattacc agttatatgc gctgttatag ttacattatc caattcatat aattcatgat
     1081 taggtttcaa aggttcattt tcaaacacat ctaaatatgc atgtcgaata actttacttt
     1141 ttaatacttc tattaagagc gcttctttaa ctatgctacc tcgtcctata tttataaaaa
     1201 gtgcttcatc tttcattaat tcaaaatgtt ttttctttag taaatgaatc gtttcttgcg
     1261 tttctggtaa agcatttata ataatgtcag catttggtaa tgtgctttca agtgattcga
     1321 tagtatatat ctcgtcaaac tcatctttgt tctgacctga cttgctcaga ccaattaaat
     1381 tcatattaaa agcctttgct aacttcgcag ttctagtagc aattgcacct gtacctaaaa
     1441 ataaaactgt ttgtcctgat aggcgtttac cagttatttt cgaatcatat atatgttgtc
     1501 gttggttatc atatgatagt ttcatctttt tataatcatc taaaatgaaa gctaaaatgt
     1561 attcagataa ttgtttagct tgaacacctt ttccattagt taaaagtatg ccgtgatctg
     1621 caatataatc taacggcaat gtatttacac ccgttgcaaa ccatgcaatc cattttaaat
     1681 tcgggcaacg tcgtaaaaat gcctcattga taccgccatc ataaccaact aatatatcta
     1741 aatcagccaa gtcgctctca ggtatttctg atgctttttt ataaaattta aaatctaaat
     1801 ctgaaaagcg ttgttgtaat tcagtttcaa cttcacgcat acgatttaac ccaacaactt
     1861 tcatatatgt caccccaaaa tgtttttaag ttcttctatt tgtgttttaa ccttaacctt
     1921 ctcgataaca tctaatactt taccttgttc atctattata aaagtcgttc ttacaatgcc
     1981 catactttct ttgccaaatg atttttttaa ctgatatacg ccagtttctt tagctaattt
     2041 aaaatcttca tctactaata aatcgaaatt caatccgtgt ttctcaataa aattttggtg
     2101 ttttttcttt gaatcaccgc ttataccata tactgcaaca tctaaatcat tgaacatttc
     2161 taaattgtct ctaaagtcac aagcttctgt ggtacaagta ggtgtattat ctctaggata
     2221 aaaatatata atcgcctttt taccttttaa tgtatcattt gtaatgacag ttccgtcttg
     2281 attttctaat ttaaatattg gaaattgttc tcctttttgc aacatataat cacctttttc
     2341 ttcattataa taattttatg atacgatatt agatgaaaat attaaattaa aagaggttga
     2401 tataaatgaa ttttagtgaa agtgaacgtt tacaacaact ttcaaacgaa tatattctag
     2461 gcggtgtcaa ttccccttct cgttcttata aagctgtagg aggcggtgca cctgttgtta
     2521 tgaaagaagg acacggtgca tatttatatg atgtcgatgg caataaattt attgattacc
     2581 ttcaagcata cggtccaatt attacggggc atgcacatcc tcatattact aaagcaattc
     2641 aagaacaagc tgctaaaggt gttttatttg gtacaccgac tgaattagaa attgaattca
     2701 gcaaaaaatt acgtgatgca attccatctc ttgagaaaat tcgctttgta aattctggaa
     2761 cagaagcagt catgacaaca attcgtgttg cacgtgcata tactaaaaga aataaaatta
     2821 taaaatttgc tggatcttat catggccatt ctgatttagt attggttgca gcaggtagcg
     2881 gcccatctca gctcggttct ccagactcag ctggtgttcc agaaagcgtc gcacgtgaag
     2941 tcattactgt acctttcaat gatattaacg cctataaaga agcaattgaa ttttggggtg
     3001 atgaaattgc cgcagtatta gtagaaccaa ttgttggtaa ctttggaatg gtaatgcctc
     3061 aacctggatt tttagaagag gttaatgaaa tttcacataa caatgggacc ctagtgattt
     3121 atgatgaagt aattactgca ttccgtttcc attacggtgc cgctcaagat ttattaggtg
     3181 ttatccctga tttaactgca tttggtaaaa ttgttggcgg tggtttacca attggaggct
     3241 atggtggacg tcaagatatt atggaacaag tagcacctct aggacctgca tatcaagctg
     3301 gtacaatggc tggtaacccg ttatctatga aagcaggtat tgcattactc gaagtactag
     3361 aacaagacgg tgtttatgaa aaattagaca gcttaggcca acaactagaa gaaggtttac
     3421 ttaaattaat cgaaaaacat aatatcacag ctacaattaa tcgtatttat ggatctttaa
     3481 cattgtactt tacagatgaa aaagtcacac attatgatca agttgaacat tctgacggcg
     3541 aagcgttcgg taaatttttc aaattaatgt taaatcaagg tatcaattta gcaccttcta
     3601 agtttgaagc ttggttctta acaactgaac atacagaaga agatattaaa caaactttaa
     3661 aagctgcaga ctatgctttt agtcaaatga aataactttt aaggaagtat atttttaatt
     3721 gaattaaaat aaagcaaatg ctcacatagt agattcattt tatatcgtta actattaacg
     3781 tttaacttga tatgtcacta tgtgagtttt tattttacaa atttatacta ttttaatctc
     3841 taactaaagt tttacatcgc tatcagtatt atgtatgatt tattttaaca actataaata
     3901 agcttgaatt ttgtaactag taagtgtata acagtaatga atacgaatat tagatattca
     3961 ataaacttaa aaggggcgtc attatttttg agactaggag ctcggatttt taaaactggt
     4021 atagccatta ttttagctat gtctatcgct tctttactac cggatgatgt tggtctgaaa
     4081 gccttagctg gtgtcagtgc tgttgttgca atgcaaccta gtatttatcg ttcatttaaa
     4141 acagtttctg atcaagcatt gggtaacatt ataggtgcca tactatctgt tacaatggta
     4201 acaattttta gtgataattt tatcattatg ggtgttaccg tcatagtact tattgctatt
     4261 ttatttaaat ttaatttagc acatgttgct actttggcga gtgtaacagc attaatcatt
     4321 atgggacaac ataccggttc tttctatatt accgcctttt acaggtttgt acttgtaatg
     4381 attggtgtca taagttcatc tctagttaac tttgtgtttc taccacctaa atttgaaaca
     4441 aaaatttact ataattcatt aaatatatcc tcagatattt ttatgtggtt taaattagta
     4501 ttaaatgata cgacggagtt taataatatt aaacaagata gtcataattt aaaacaacgt
     4561 gtcgaaaagt tagagaaaat ttatgattac tatagcgaag aacgtcctat tacaaaaaaa
     4621 catattcacc aacagaatag aaagaaaata cttttcagag aagtagttca gacgactaga
     4681 caagcttatg aagtattaaa caaattgtcg cgttatcaaa acgacttata tttacttaat
     4741 aacaatttcc tattacaaat caaattagat ttagattcat taactgcttt tcacgagcaa
     4801 attttagcta gcctttctaa aaaagcacgt tataatgtga cacatgttga ttatgagtta
     4861 gataatccgc agaaaaaaga tttattgtcc acatttcagc atgagttaat aaatcatcct
     4921 tatcaaacgg aatattcgtt tgccaatgtt atgcaaattg ttgcagccat cgaagagtat
     4981 cgacaccatt tagagcattt agaccgcata agaattagct ttttcacata tcatagatct
     5041 gatgcagaca ttgaaattgt tgaagaagac tttgatttat aacattaaaa caattttatc
     5101 aattaatgcc acaatgagat aaactaatat tgaatctcga aattaaattt aaaaaagcag
     5161 taagattatt ttcaattaag aaaatctctt actgcttttt ctatgttatg accccaccat
     5221 cctgttacaa attttctctt caagattgta ctgaagtcaa acaactaata ctttataagt
     5281 tttgaatgct atataaatgc tcgtaagcac cttgttttgc aatcaattca cgatgcgtac
     5341 ctgtttcaac aatatgtcca ttttcaatta cgacaatttt gtcagcatgt gtaatagtgg
     5401 acaagcgatg cgctacgata agtgtcgttc gatctttact caacacatct aatgcttctt
     5461 gaataatgga ttcactttct aaatcaagtg cacttgttgc ttcatccaag ataagaattg
     5521 gcggattatt taaaaatatt ctagcaatcg ataatctttg tttttgacca cctgataatt
     5581 taacacctcg ttcacctact tcagtgtcat atccctgtgg caagttcata ataaagtcat
     5641 gtgcattagc cattttcgcc gcttcaacta cttcttcatc tgttgctgtt ggacgaccaa
     5701 gtaaaatatt ttccttaact gtatcagaga ataaaatatt atcctgttgc accaatccta
     5761 tttgatttct taaacttccc gttaaaaaat ctttaatgtt gtgaccatct attaaaattt
     5821 gcccagaagt tacatcgtaa aatctcggta ttaagttaat taatgttgat ttaccaccac
     5881 cactcatacc tacgaaagca actgtttctc ctttttcaat actcaaatta atatctttta
     5941 aaattggagc ttcgttatcg ttatattgaa aactaacatg atcaatatca atacgacctt
     6001 gtttaatttc aataggttga gcaccaacac catttttgat gtcataatct tcatcaatta
     6061 attggaatac acggtccatt gaagcaaaac tttgcgttaa agttgtaaat gatgcgacta
     6121 aacgacgtaa aggcccgaac aataactcta agtatccaac aaatgctgca agtgtaccta
     6181 ctgtgattga tccagaaata gcaagatatg caccaacacc gatgacaata attggtccaa
     6241 tatctgtaac tgtattaatt gcggcaaagg aataggcatt ccatcttgta tgtttcaacg
     6301 cacgtgttag gaaattagta ttctttttat caaagttttt cgcttcattg tcttcaatcg
     6361 caaaactttt aacgactgaa ataccttgaa cacgttcatg caagaatcct tgaacctcag
     6421 ctaatgcttg agatctttca cgtgtcaatt ttcttaatct tccaaagaaa acgtacaccg
     6481 ttaaaatgta aaatgggaag ataaacagtg ctgctaaagt caatttcaca tctaaaaaga
     6541 acattatgga tagtgcaata ataattgtta tacaatctaa ccaaatattc attaacccgg
     6601 ttaaaatgaa atcttttgtt tgttcaacat cattaatcac tctagatatt acttgaccta
     6661 cttgattatt agcataaaat ctcgcactta aagcttgtaa atggttgtat aactttttac
     6721 gtatatcata caatatttta ttacttgtcc attgcgccaa atattgacgt ataaattcaa
     6781 ttggtggtct cactattaca aaaataaata atgcgatacc aatggcaata gttaaatgat
     6841 gaactttttc atcagtcgtt agtgcgtggt tattaatcac gccatctatt gcatatttaa
     6901 ttaatagtgg tataagcatt ggtataccaa acttaattat cccaacaata atcgttgcaa
     6961 aaatacgata tttgtatggc ttaacaaatt gcaaatatcg tttaatcata caatcccccc
     7021 taatctattg ccctatccta ttcataagca taaaaatgaa tagaggttgg atacataatt
     7081 tgtagatgta aattcttctt acaa
//
LOCUS       DAMQYT010000133         7058 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMQYT010000133 DAMQYT010000000
VERSION     DAMQYT010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.133"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            48..293
                     /gene="yaaA"
                     /locus_tag="PGA33_001996"
     CDS             48..293
                     /gene="yaaA"
                     /locus_tag="PGA33_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDG6473800.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            290..1402
                     /gene="recF"
                     /locus_tag="PGA33_001997"
     CDS             290..1402
                     /gene="recF"
                     /locus_tag="PGA33_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDG6473801.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            1412..3346
                     /gene="gyrB"
                     /locus_tag="PGA33_001998"
     CDS             1412..3346
                     /gene="gyrB"
                     /locus_tag="PGA33_001998"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDG6473802.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            3383..6046
                     /gene="gyrA"
                     /locus_tag="PGA33_001999"
     CDS             3383..6046
                     /gene="gyrA"
                     /locus_tag="PGA33_001999"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDG6473803.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDSSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFENLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEE"
     gene            complement(6133..6945)
                     /locus_tag="PGA33_002000"
     CDS             complement(6133..6945)
                     /locus_tag="PGA33_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDG6473804.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
BASE COUNT         2425 a         1093 c         1450 g         2090 t
ORIGIN      
        1 tgtgacaaaa aaaggtataa tatattaatg acatacaaag aaatggagtg attattttgg
       61 ttcaagaagt tgtagtagaa ggagacatta atttaggtca atttctaaaa acagaaggga
      121 ttattgaatc tggtggtcaa gcaaaatggt tcttgcaaga cgttgaagta ttaattaatg
      181 gagtgcgtga aacacgtcgc ggtaaaaagt tagaacatca agatcgtata gatatcccag
      241 aattacctga agatgctggt tctttcttaa tcattcatca aggtgaacaa tgaagttaaa
      301 tacactccaa ttagaaaatt atcgtaacta tgatgaggtt acgttgaaat gtcatcctga
      361 cgtgaatatc ctcattggag aaaatgcaca aggaaagaca aatttacttg aatcaattta
      421 taccttagct ttagcaaaaa gtcatagaac gagtaatgat aaggaactca tacgttttaa
      481 tgctgattat gctaaaatag aaggtgagct tagttataga cacggcacga tgccattaac
      541 aatgtttata actaaaaaag gtaaacaagt caaagtgaat cacttagagc aaagtcgtct
      601 aactcaatat attggacacc tcaatgtggt tctatttgcg ccagaagatt tgaatattgt
      661 aaaaggctct cctcaaataa gacgacgctt tatagatatg gagttgggcc aaatttctgc
      721 tgtttactta aatgatttag ctcaatacca acgtatttta aagcaaaaga ataattactt
      781 aaagcagtta caattaggcc aaaaaaagga cttaacaatg ttggaagtat taaatcagca
      841 gtttgctgaa tatgcaatga aagtaactga taaacgtgca cattttattc aagagctaga
      901 gtcgttagct aaaccgattc atgctggtat cacaaatgat aaagaagcgt tgtcgctgaa
      961 ttatttacct agtcttaaat ttgattatgc tcaaaatgaa gcggcacgac ttgaagaaat
     1021 tatgtctatt cttagcgata atatgcaaag agaaaaagaa cgaggcatta gcttattcgg
     1081 accacatcga gatgatataa gttttgatgt gaatggcatg gatgctcaaa catatggttc
     1141 tcaaggacag caacgtacaa cggctttgtc cattaaatta gctgaaattg agttaatgaa
     1201 tatcgaagtt ggggaatatc ccatcttatt attagacgat gtactcagtg aattagatga
     1261 ttcgcgtcaa acgcatttat taagtacgat tcagcataaa gtacaaacat ttgtcactac
     1321 gacatctgta gatggtattg atcatgaaat catgaataac gctaaattgt atcgtattaa
     1381 tcaaggtgaa attataaagt aacagaaagc gatggtgact gcattgtcag atgtaaacaa
     1441 cacggataat tatggtgctg ggcaaataca agtattagaa ggtttagaag cagtacgtaa
     1501 aagaccaggt atgtatatag gatcgacttc agagagaggt ttgcaccatt tagtgtggga
     1561 aattgtcgat aatagtatcg atgaagcatt agctggttat gcaaatcaaa ttgaagttgt
     1621 tattgaaaaa gataactgga ttaaagtaac ggataacgga cgtggtatcc cagttgatat
     1681 tcaagaaaaa atgggacgtc cagctgtcga agttatttta actgttttac atgctggtgg
     1741 taaatttggc ggtggcggat acaaagtatc tggtggttta catggtgttg gttcatcagt
     1801 tgtaaacgca ttgtcacaag acttagaagt atatgtacac agaaatgaga ctatatatca
     1861 tcaagcatat aaaaaaggtg tacctcaatt tgacttaaaa gaagttggca caactgataa
     1921 gacaggtact gtcattcgtt ttaaagcaga tggagaaatc ttcacagaga caactgtata
     1981 caactatgaa acattacagc agcgtattag agagcttgct ttcttaaaca aaggaattca
     2041 aatcacatta agagatgaac gtgatgaaga aaacgttaga gaagactcct atcactatga
     2101 gggcggtatt aaatcgtacg ttgagttatt gaacgaaaat aaagaaccta ttcatgatga
     2161 gccaatttat attcatcaat ctaaagatga tattgaagta gaaattgcga ttcaatataa
     2221 ctcaggatat gccacaaatc ttttaactta cgcaaataac attcatacgt atgaaggtgg
     2281 tacgcatgaa gacggattca aacgtgcatt aacgcgtgtc ttaaatagtt atggtttaag
     2341 tagcaagatt atgaaagaag aaaaagatag actttctggt gaagatacac gtgaaggtat
     2401 gacagcaatt atatctatca aacatggtga tcctcaattc gaaggtcaaa cgaagacaaa
     2461 attaggtaat tctgaagtgc gtcaagttgt agataaatta ttctcagagc actttgaacg
     2521 atttttatat gaaaatccac aagtcgcacg tacagtggtt gaaaaaggta ttatggcggc
     2581 acgtgcacgt gttgctgcga aaaaagcgcg tgaagtaaca cgtcgtaaat cagcgttaga
     2641 tgtagcaagc cttccaggta aattagccga ttgctctagt aaaagtcctg aagaatgtga
     2701 gattttctta gtcgaagggg actctgccgg ggggtctaca aaatctggtc gtgactctag
     2761 aacgcaggcg attttaccat tacgaggtaa gatattaaat gttgaaaaag cacgattaga
     2821 tagaattttg aataacaatg aaattcgtca aatgatcaca gcatttggta caggaatcgg
     2881 tggcgacttt gatctagcga aagcaagata tcacaaaatc gtcattatga ctgatgccga
     2941 tgtggatgga gcgcatatta gaacattgtt attaacattc ttctatcgat ttatgagacc
     3001 gttaattgaa gcaggctatg tgtatattgc acagccaccg ttgtataaac tgacacaagg
     3061 taaacaaaag tattatgtat acaatgatag ggaacttgat aaacttaaat ctgaattgaa
     3121 tccaacacca aaatggtcta ttgcacgata caaaggtctt ggagaaatga atgcagatca
     3181 attatgggaa acaacaatga accctgagca ccgcgctctt ttacaagtaa aacttgaaga
     3241 tgcgattgaa gcggaccaaa catttgaaat gttaatgggt gacgttgtag aaaaccgtag
     3301 acaatttata gaagataatg cagtttatgc aaacttagac ttctaagcgc tgtgaactga
     3361 acttttgaag gaggaactct tgatggctga attacctcaa tcaagaataa atgaacgaaa
     3421 tattaccagt gaaatgcgtg aatcattttt agattatgcg atgagtgtta tcgttgctcg
     3481 tgcattgcca gatgttcgtg acggtttaaa accagtacat cgtcgtatac tatatggatt
     3541 aaatgaacaa ggtatgacac cggataaatc atataaaaaa tcagcacgta tcgttggtga
     3601 cgtaatgggt aaatatcacc ctcatggtga ctcatctatt tatgaagcaa tggtacgtat
     3661 ggctcaagat ttcagttatc gttatccgct tgttgatggc caaggtaact ttggttcaat
     3721 ggatggagat ggcgcagcag caatgcgtta tactgaagcg cgtatgacta aaatcacact
     3781 tgaactgtta cgtgatatta ataaagatac aatagatttt atcgataact atgatggtaa
     3841 tgaaagagag ccgtcagtct tacctgctcg attccctaac ttattagcca atggtgcatc
     3901 aggtatcgcg gtaggtatgg caacgaatat tccaccacat aacttaacag aattaatcaa
     3961 tggtgtactt agcttaagta agaaccctga tatttcaatt gctgagttaa tggaggatat
     4021 tgaaggtcct gatttcccaa ctgctggact tattttaggt aagagtggta ttagacgtgc
     4081 atatgaaaca ggtcgtggtt caattcaaat gcgttctcgt gcagttattg aagaacgtgg
     4141 aggcggacgt caacgtattg ttgtcactga aattcctttc caagtgaata aggctcgtat
     4201 gattgaaaaa attgcagagc tcgttcgtga caagaaaatt gacggtatca ctgatttacg
     4261 tgatgaaaca agtttacgta ctggtgtgcg tgtcgttatt gatgtgcgta aggatgcaaa
     4321 tgctagtgtc attttaaata acttatacaa acaaacacct cttcaaacat catttggtgt
     4381 gaatatgatt gcacttgtaa atggtagacc gaagcttatt aatttaaaag aagcgttggt
     4441 acattattta gagcatcaaa agacagttgt tagaagacgt acgcaataca acttacgtaa
     4501 agctaaagat cgtgcccaca ttttagaagg attacgtatc gcacttgacc atatcgatga
     4561 aattatttca acgattcgtg agtcagatac agataaagtt gcaatggaaa gcttgcaaca
     4621 acgcttcaaa ctttctgaaa aacaagctca agctatttta gacatgcgtt taagacgtct
     4681 aacaggttta gagagagaca aaattgaagc tgaatataat gagttattaa attatattag
     4741 tgaattagaa gcaatcttag ctgatgaaga agtgttatta cagttagtta gagatgaatt
     4801 gactgaaatt agagatcgtt tcggtgatga tcgtcgtaca gaaattcaat taggtggatt
     4861 tgaaaactta gaggacgaag acttaattcc agaagaacaa atagtaatta cactaagcca
     4921 taataactac attaaacgtt tgccggtatc tacatatcgt gctcaaaacc gtggtggtcg
     4981 tggtgttcaa ggtatgaata cattggaaga agattttgtc agtcaattgg taactttaag
     5041 tacacatgac catgtattgt tctttactaa caaaggtcgt gtatacaaac ttaaaggtta
     5101 cgaagtgcct gagttatcaa gacagtctaa aggtattcct gtagtgaatg ctattgaact
     5161 tgaaaatgat gaagtcatta gtacaatgat tgctgttaaa gaccttgaaa gtgaagacaa
     5221 cttcttagtg tttgcaacta aacgtggtgt cgttaaacgt tcagcattaa gtaacttctc
     5281 aagaataaat agaaatggta agattgcgat ttcgttcaga gaagatgatg agttaattgc
     5341 agttcgctta acaagtggtc aagaagatat cttgattggt acatcacatg catcattaat
     5401 tcgattccct gaatcaacat tacgtccttt aggccgtaca gcaacgggtg tgaaaggtat
     5461 tacacttcgt gaaggtgacg aagttgtagg gcttgatgta gctcatgcaa acagtgttga
     5521 tgaagtatta gtagttactg aaaatggtta tggtaaacgt acgccagtta atgactatcg
     5581 cttatcaaat cgtggtggta aaggtattaa aacagctacg attactgagc gtaatggtaa
     5641 tgttgtatgt atcactacag taactggtga agaagattta atgattgtta ctaatgcagg
     5701 tgtcattatt cgactagatg ttgcagatat ttctcaaaat ggtcgtgcag cacaaggtgt
     5761 tcgcttaatt cgcttaggtg atgatcaatt tgtttcaacg gttgctaaag taaaagaaga
     5821 tgcagaagat gaaacgaatg aagatgagca atctacttca actgtatctg aagatggtac
     5881 tgaacaacaa cgtgaagcgg ttgtaaatga tgaaacacca ggaaatgcaa ttcatactga
     5941 agtgattgat tcagaagaaa atgatgaaga tggacgtatt gaagtaagac aagatttcat
     6001 ggatcgtgtt gaagaagata tacaacaatc atcagatgaa gaataataaa aaataagact
     6061 tccctatatg taggggagtc ttatttttat gctagaaagt aatgctttac tatattcaat
     6121 gattagtaat gactaacttt ctaattgttt cattgcgtaa ggtatttcat tgataagtct
     6181 tgatggtggc accacataca tatcttttgc aaggttttcg ccaataaaac tatgtgtata
     6241 tgtggcactc ataaccgctt cttttaagtt atcaaattga ccgacaaagc ttgtaatcat
     6301 accagcaagt gtatcgccca taccaccagt cgccattgct gggctaccga ttgtcaattt
     6361 aaagtcttca tctttaaaga aaatttcagt accatgtttt ttaagtacaa cagttgcacc
     6421 taaacgatca actgcttcac gattacgctc atatgtctgt tcctcaatag gaataccact
     6481 taatcgttcc cattctttga ggtgtggtgt aaagatcaca cgacatgtag gtaattgcgg
     6541 tttcagttta ctaaagattg taatcgcatc gccgtctacg attaaatttt gatgcggttg
     6601 tatattttgt agtaggaatg taatggcatt atttcctttg aaatcaacgc caagacctgg
     6661 accaattagt atactgtcag tcatttcaat cattttcgtc aacattttcg tatcattaat
     6721 atcaataacc atcgcttctg ggcaacgaga atgtaatgct gaatgatttg ttggatgtgt
     6781 agctacagtg attaaaccac taccgctaaa tacacatgca cgagccgcta acataatggc
     6841 accacctaag ttagcagatc caccaattaa taaaattttg ccataatcac ctttatgtga
     6901 atcttcttta cgcttaggaa tgttaataga atttaacgtt tccatagtga tataacctcc
     6961 catgtaaaag ccttttccga atttattcaa ttttaaaaat atatagtaac ttttaacaaa
     7021 atgtattata aatttctgaa ttcattatta tttgtcgt
//
LOCUS       DAMQYT010000134         7032 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMQYT010000134 DAMQYT010000000
VERSION     DAMQYT010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.134"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..1261
                     /locus_tag="PGA33_002001"
     CDS             <1..1261
                     /locus_tag="PGA33_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDG6473805.1"
                     /translation="STTIIGALLLYLPFTGKKPISFLDALFIASSAFTVTGLSPVDIG
                     SQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNRFLIMVTWNIDEPGGVI
                     KLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTSVSAFNNAGFALFKNNL
                     IDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLHSKLVLTTTSILIIIGA
                     ITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIASINKSTALMLMLLMFI
                     GGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISDKHIKLSIVTINISFLF
                     ISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYHGITKIIIIFVMLCGKV
                     GLLTLLRTFIPPKSPKNYRYTKGQIYL"
     gene            complement(2114..2557)
                     /locus_tag="PGA33_002002"
     CDS             complement(2114..2557)
                     /locus_tag="PGA33_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6473806.1"
                     /translation="MSIITRLFNNSDFEKLNQLCKLYDDLGYPTNENDLKKRLKKITN
                     HDDYFLLLLIKENKIIGLSGMCKMMFYEKNAEYMRILAFVIHSEFRKKGYGKRLLADS
                     EEFSKRLNCKAITLNSGNRNERLSAHKLYSDNGYVSNTSGFTKQL"
     gene            complement(2722..3099)
                     /locus_tag="PGA33_002003"
     CDS             complement(2722..3099)
                     /locus_tag="PGA33_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473807.1"
                     /translation="MFISLAIWSINSMGNKQCISLNLLLSDDTFAVISVMTTLGTAIL
                     GGIAPSIYGIFTSPIDKKSSFRAISDWEQEQRNNVKDLMMLLPFTLLSILIVIFIFQF
                     AYSKLFNTIAIIIFIIILVVMFF"
     gene            complement(3417..4007)
                     /locus_tag="PGA33_002004"
     CDS             complement(3417..4007)
                     /locus_tag="PGA33_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDG6473808.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYNANSAHVTACRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGNVPIFINIGEWDGG
                     DEGLDKAVKDVSNDNPNHTVIVDDIPLEDYESIRFL"
     gene            complement(4004..4216)
                     /locus_tag="PGA33_002005"
     CDS             complement(4004..4216)
                     /locus_tag="PGA33_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HDG6473809.1"
                     /translation="MTDDNVNDHIIKNHIEMIVDRLATDKEFYIFDSLIQGLSYQDIS
                     SALDCSEQSVILWYETILDKIVGVIK"
     gene            4277..4823
                     /locus_tag="PGA33_002006"
                     /pseudo
     CDS             4277..4823
                     /locus_tag="PGA33_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(4918..6534)
                     /gene="groL"
                     /locus_tag="PGA33_002007"
     CDS             complement(4918..6534)
                     /gene="groL"
                     /locus_tag="PGA33_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDG6473810.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEEGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(6610..6894)
                     /gene="groES"
                     /locus_tag="PGA33_002008"
     CDS             complement(6610..6894)
                     /gene="groES"
                     /locus_tag="PGA33_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDG6473811.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
BASE COUNT         2291 a         1329 c          879 g         2533 t
ORIGIN      
        1 ctctacaact atcataggtg cacttttatt gtatttacct tttactggta aaaagccaat
       61 atctttttta gatgccctat ttatagcttc aagtgcattt acagttactg gcttgtcccc
      121 agttgatata ggatcacagt ttaatatact tggtgagata gtaatactat tattaattca
      181 aataggtggt ctgggtatcg tgaccgtaac cctattgaca ctagtatttt taaatagaaa
      241 gatatcaatg aaaaatagat tcttgattat ggttacatgg aatattgacg aacctggtgg
      301 tgttattaag ctaattaaac acttggctat ttatagttta gtcactgaat taattggtat
      361 gatttgtttg tgtttatctt ttataccaaa atttggtata ggcaaaggtt tatttttaag
      421 cttattcaca tcagtatcag cttttaataa tgctggattt gcccttttta agaataactt
      481 aatagattat tctagtgatc caattgtcat tattacaatc tcaatactca taatatttgg
      541 aggtattgga cattttgtcg tgatagactt tattaattgt aaaaaattga gtaaattatc
      601 tttacattct aaattagtct taactacaac tagtatccta ataattatag gagctattac
      661 attcttttta ttagaacagt ttaatactat gcaacatatg ggactagttg aaaaaatcgg
      721 aaattctttt ttccaatcag taacaacacg aacagcgggt tttaacagta tagatatagc
      781 aagcattaac aaatctaccg cattaatgtt aatgctactt atgtttattg gtggtgcccc
      841 tctcagtgca gctggaggaa ttaaaataac tacttttgca gttgcgttta tttttgtact
      901 aaattatata cgtaaagaaa ataatgtttc agtattcaat aaagaaatat ctgacaaaca
      961 tataaaacta tctattgtta ccattaatat ctcatttcta tttatcagca tcattacttt
     1021 tatattatcg ataattaatc cgaacatatc attaatcaag ttattattcg aagtggtttc
     1081 tgcattcgga acagtagggt taagtatgaa ccttaccaca gaatatcatg gtattactaa
     1141 aataattatt atattcgtta tgctttgcgg taaagtagga ctattaactt tattaagaac
     1201 atttatacca ccaaaaagtc ctaaaaatta ccgctacact aaaggacaaa tttatctata
     1261 aaataactat ccatctttaa taagaaatga actagtgttt taatctatta cattaaaaaa
     1321 actacctaaa caggtagttt tttatttatt ttaggccgat taaatgtctt atgtctttaa
     1381 ttaagtaaaa tgcacaagta cacctttaaa aagatttact caaaactaaa ctatacctag
     1441 ttaaccgctt gtgattaaga aattttaaat gtggtttctt ttaattatgt caaccaccca
     1501 taaggatttt aatcaagcac tcattcattc ctgcactcgc aatatgcatt acttatagaa
     1561 gtggatgcac tctcatatta tggcaatacc ataactcccg caccataatg atttaaatac
     1621 cattatgaat ctcaattaca aattagcaca taaaaaagac gcctcccata agtctagtca
     1681 ccaaatggaa aacgtctcgt aattaaaata tatttgcgtg cattctaatt tgtacttaga
     1741 atacatcata ctcgttcata atgtaatttt ctaattagtc aaaactatat acagttttac
     1801 atcattcccg gcattatgca ctaataaaaa cttactttaa tacacgcttt aaagattatt
     1861 aaatcagcgt tttaattttt agacaacgct attattttta gtttttcaat tctattatgt
     1921 cataattatg tcactcaaaa actgttttcc aacattgatt tattgttgga aaatctcgaa
     1981 aatctagtct atttctccaa caatcgaaga attatgccct tttttctccc tttaaataag
     2041 tcataatacg aggcatacat gcaacattta caataaaata tgttcctaca tcgtattata
     2101 cgaatgctca cacttatagt tgtttagtaa acccagatgt attgctaaca tacccattat
     2161 cactatatag tttatgtgca gatagtcttt catttctatt accactattt agtgttattg
     2221 ctttacaatt caaccgttta gaaaattctt cagaatcagc taataatctc tttccataac
     2281 cttttttcct aaattcagaa tgtataacaa acgcaaggat tctcatatac tctgcatttt
     2341 tttcgtaaaa catcatttta cacataccac ttaaaccaat tattttattt tcttttatca
     2401 aaagcagtag gaagtaatca tcatgattcg ttattttctt tagtctcttt tttaaatcat
     2461 tctcatttgt tggataacct agatcatcat ataatttaca tagttgattt aatttttcaa
     2521 aatcactgtt attaaacaat cttgtaatta tgctcatata acccacccta aaaatattat
     2581 gtacctattg ttacatacaa tagatacgca aaatcatttc tcactgcaaa tcaggacgtt
     2641 tctcagcgta aacaaatcat ttctattctt ttgaaaaata tctgcatatc ttcacagtaa
     2701 ttttataaca gattaataaa attaaaaaaa cataacaaca agtattataa taaatattat
     2761 aatagctatc gtattaaata gtttagaata tgcaaactga aaaataaaaa tcacaattaa
     2821 aatactaagt aatgtaaatg gtaatagcat catcagatct ttaacattat ttctttgttc
     2881 ttgctcccaa tcgctaatag ccctaaaact acttttttta tctataggcg atgtaaaaat
     2941 accatatatt gaaggtgcta tacctcctaa aatagcagtt cccaaagttg tcattactga
     3001 aattactgcg aaagtatcat ccgaaagcaa taaattcaaa ctaatgcatt gtttattacc
     3061 catcgaattt attgaccaaa tagctagaga aataaacaac ccaaaattta aaataaatga
     3121 tatagtaata gcaattgttt acaaaacacg aaatttttca tttttattta tattatccat
     3181 ttttctccct ttttcttaaa tcattttatt atatattaca ataatcaatc tgaaatgttg
     3241 atgtaatttg aaaaaaatat catactttta ctcctgaaaa cctccctaaa tcatcaatat
     3301 gaaaatcagt attaggatat tgcgtttaca actcttttaa atctcactca ttcttctcat
     3361 cgtcttaacc gatactatca ataaatactg gcaacaccat aaaatatata cccaatttat
     3421 aaaaacctaa ttgactcata atcttctaac ggtatatcat ccacaatcac agtatgatta
     3481 ggattatcgt tagatacatc tttcactgcc ttatctaacc cctcatcacc tccgtcccat
     3541 tcaccaatat taatgaatat aggtacatta ccgtttatat catgcttatc tgtaaataat
     3601 ttatgatatt tacccaacat atcacgagct tttaaacgat cactaggttt tattggtacc
     3661 tctatcagtt caacatgttc attatagact aattgtactt tgccactttg tggattttct
     3721 ttatattctc cacgcttgac tacaacttct ttcgtttctg tttcatcacc gactgccgca
     3781 ttcgttagca catgtaataa ctcttttgcg gttaatacat tctcatctat aatcttatct
     3841 ttttgttctt gtatatattg cttgatgtgc ggctttttca ataacctaca cgctgtcaca
     3901 tgtgcgctat ttgcgttata tcctgctttt atggcacttt gtgttacatt cagtgttcta
     3961 atatactcat tcacaaaacg tgcttgcttt gcagttaact cactcatttt atcaccccca
     4021 caattttatc taatatggtt tcataccata atattacaga ttgttctgaa caatctaagg
     4081 cactactaat atcttgataa ctaagtcctt gtataaggga gtcaaaaata taaaactctt
     4141 tatcggtcgc taatctgtca acaatcattt ctatgtgatt ctttataata tgatcattga
     4201 cattatcgtc tgtcatcaat tcgtcagaat cttcatcacc tattaaaaag aaatcatcag
     4261 tatttatttc atcatcgccc cgttaactag ctttgaagtc tttagcacac ttgcatatac
     4321 cggctgtcgt gctggcgaga tactagcatt gaagtggtct gatattgatt ttgaaaacaa
     4381 cacgattagt attactaaaa catattacaa tccaaataat aacaagaaga aatatcagat
     4441 acttccccct aaaactgaaa gttctatcgg taagatttcc gttgacccaa atgtaataaa
     4501 ggtgttgcgt gattataaga taaacgttca aaataattgg aaaaacgaat tatataatga
     4561 taaccattgt aatgaaaaaa ctatcaatgt ggatacaaac cattatgtca cgtaccagca
     4621 tactcaaaaa tatctctacg cactcattca gatacacaca ttgcgcattg ctcatagaat
     4681 caggtgtaca cattaaagaa atacaagaac gattacggca taaagatatc aataccacta
     4741 tgaatatcta tgctaaaatc acaaattcat acaaaaaaga cgcctcccat aagtttagtc
     4801 accaaatgga agacgtctcg taattaaaat atatttgcgt gcattctaat ttatacttag
     4861 aatgaatcat actcgtgcat aatgtaattt tctagttagt caaaactata aacagtttta
     4921 catcattcct ggcatgccac ccatgttagg ttggtcatta tttttttctg gaattgatgc
     4981 tacaaccgct tcagtcgtta agaacattgc tgcaacactt gcagcatgtt gtaatgctga
     5041 gcgtgttact ttagttggat caacgatacc ttcttctaac atattaaccc actcgtttgt
     5101 agcagcgtta aaaccaacac ccggctctgc gtttttcaaa cgttctacaa taacagaacc
     5161 ttctaatcct gcattttcag caatttgacg aactggtgca gttaatgctt taagtacaat
     5221 atttacacct gtttcaatgt caccttcagc ttcaatttca cttacttttt ggtaaacatt
     5281 tactaatgca gtaccaccac ctgcaacaat accttcttca actgctgcac gtgtagaatt
     5341 taatgcatct tcaatacgta atttacgttc tttaagctct gtttcacttg ctgcacctac
     5401 tttgataact gcaacaccac ctgctaattt agctaagcgc tcttgtaatt tttcacgatc
     5461 aaagtcagat tcagtttctt caatttgaga tttcaattgg ctaacacgtg catcaatgct
     5521 gttttcgtca ccgtcaccat caacaacagt ggtattatct ttagttactt ctactttact
     5581 tgcagtacct aacatatcaa ttgatgcatc ttttaaatct aagcctaaat catcagtaat
     5641 cacttgcgca ccagttaaaa tagctaaatc ttcaagcatc gcttttctac gatcaccaaa
     5701 accaggtgct tttactgcaa cagctgtaaa tgtgccacgc atacggttta gcacgatatt
     5761 tgttaatgca tcgccttcaa cttcatcagc tacaattaag attggacgat tagattgaac
     5821 cacttgttct aataaaggta agatatcttg gaaagacgag attttcttat ctgttactaa
     5881 aatgtatggg cgttctaatt cagcaaccat tttatctgaa tcagtaacca tatacggtga
     5941 ttgataacca cgatcaaatt gcataccttc aaccacttct agttcagtgt ttagtccatt
     6001 tgattcttca attgtaatga caccatcgtt acctactttt tccatagctt cagaaatata
     6061 acgtccaatt tcttcatctg ctgctgaaat cgcacctact tgcgcaattt catttttatt
     6121 ttcaactttt tgagaatttt catgtaacgc ttcaacagca actttaactg ctttgtcgat
     6181 accttgtcgt aaaccaactg ggttcgcacc acttgtaaca tttttcaagc cttcttgaat
     6241 cattgcttga gctaatactg ttgcagttgt cgtaccgtca ccagcaattt catttgtctt
     6301 atttgcgact tcttgaacta gtttagcccc catattttca tatggatctt ctaattcgat
     6361 ttctttagca atcgtcacac catcattcgt aattaaaggt gctgtaaact ctttatctaa
     6421 tacaacatta cgtcctttag gaccaatcgt tactttaact gcatttgcaa gttggtcaac
     6481 accacgtaac attgcttgac gtgcatcttc agagaatttc aattgtttaa ccataaatga
     6541 taaacctcca tttgtttagt tgtgtttcat tttcgttcta tttaatttat ttatgaatta
     6601 agttctgtat tattcaataa ctgctaaaat atcttcttca tttaatacca gatatgtttc
     6661 attatctcgt ttaacttctg taccagcata ttgttggaac acgacacggt ccccttcttt
     6721 cacttcagga gtcactcttg taccatcatt taataggcgt ccagttccta ctgcaacgat
     6781 aacgccttcg tttgattttt ctttagcact atcagttaaa acaataccac ttttagttgt
     6841 ttgttcttgt tcttttttct caataatcac acgatttcca attggtttta gcatgattgt
     6901 tcctccttaa aaaacctaaa gtttagcact taacattaaa gagtgctaac atacatttat
     6961 aataatcaaa tttggtcaaa atttcaagtc ataacctttt aattaagttt tgtaacttca
     7021 agttatttac ga
//
LOCUS       DAMQYT010000135         6790 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMQYT010000135 DAMQYT010000000
VERSION     DAMQYT010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.136"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            218..619
                     /locus_tag="PGA33_002009"
     CDS             218..619
                     /locus_tag="PGA33_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDG6473812.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(739..1302)
                     /locus_tag="PGA33_002010"
     CDS             complement(739..1302)
                     /locus_tag="PGA33_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDG6473813.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(1299..2252)
                     /locus_tag="PGA33_002011"
     CDS             complement(1299..2252)
                     /locus_tag="PGA33_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDG6473814.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            2362..3543
                     /locus_tag="PGA33_002012"
     CDS             2362..3543
                     /locus_tag="PGA33_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDG6473815.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            3831..6248
                     /locus_tag="PGA33_002013"
     CDS             3831..6248
                     /locus_tag="PGA33_002013"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDG6473816.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYK
                     EKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYATNPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLAIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            6270..6581
                     /locus_tag="PGA33_002014"
     CDS             6270..6581
                     /locus_tag="PGA33_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDG6473817.1"
                     /translation="MKSITTDELKNKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
BASE COUNT         2284 a          965 c         1295 g         2246 t
ORIGIN      
        1 tgtactatta tcttatttct aaatattaac tctattgatt attggttttt atacttattt
       61 aattttattc aactttgaca attgaataga aagcaagttt atttacactt gtagttttat
      121 gcataagtta gcacatacaa gttttgggat tgttgggatg tttgttaata cttgtatagt
      181 agctaaatat gtgattatta attgggagat gtttagcatg aaaaaagtaa ataacgacac
      241 tgtatttgga attttgcaat tagaaacact tttgggtgac attaactcaa ttttcagcga
      301 gattgaaagc gaatacaaaa tgtctagaga agaaatttta attttactaa ctttatggca
      361 aaaaggttct atgacgctta aagaaatgga cagatttgtt gaagttaaac cgtataagcg
      421 tacgagaacg tataataatt tagttgaatt agaatggatt tacaaagagc gtcctgttga
      481 cgatgaaaga acagttatta ttcatttcaa tgaaaagtta caacaagaga aagtagagtt
      541 gttgaatttc atcagtgatg cgattgcaag tagagcaaca gcaatgcaaa atagtttaaa
      601 cgcaattatt gctgtgtaag tttaatagca taaaaagagg ttttcattaa gttgaaaacc
      661 tctttttgtt gttggcatta atttttcaaa tgttgactac tcaatcctaa attataaata
      721 gtatagcgca gcaaatgctt aagaaatttt ttctatggca caaatgaatg gagcatgatt
      781 acgttggttt aaaaattgat attgcaaaac ttgcgcatgc ttttgatcca aagtactcaa
      841 gtaatcaagc aatgcatgct tctcaatttg tccttcgcta tgaccatgat atataacaag
      901 tacaataata ccttcaattg acattaatga tagcaatgaa ttaatagctt ggattgtcgt
      961 gtcaggcttt gtcacgatag atttatcacc tttaggcaaa taacctaggt taaagatggc
     1021 tgcatcaata tgacctttat gtgcatcatt tatatgatgt tcaatatttt catgtccatc
     1081 ttttattaaa gaaacatgat tgaaatcctt aactttatca cgtgtatttt ccaaagctaa
     1141 atcttgaatg tcgaaaccat aaacatgtcc ttctggtact tgttcggcta aaaataaagt
     1201 gtcattgccg ttaccgcaag ttgcgtctac aacaatactt tctggtgtta tatgttgttt
     1261 aataagtgtt tttgaaaaag ggagtatacg ttctaatttc attgcttcac cttagacttg
     1321 taacgcaagc cttgataaga atttctacgt gctaattcag catcgatgcc atttaatact
     1381 tcccatttat taacactcca cattggacct accatgatat ctattggacc atcaccggta
     1441 attcggtgaa cgatcatttc agggggaatc acttctaatt ggtccacaac taggtttgtg
     1501 tactcttctt gagtcataaa agttaataaa cctttatcgt attgttttac catcggtgta
     1561 ccttttaaca aatgaagtaa atgaatttta ataccttgta catccatttg tgcaacttct
     1621 ttggcagtag ccatcatcat gtcatagtct tcgccaggta agccattaat gatgtgtgta
     1681 catacattga tattatgctt acgtaatttt gccacaccat cataataagt tttcatatca
     1741 tgggcacgat tgattaaatc agatgttgac tgatggattg tttgtagtcc taattcaacc
     1801 cataagtatg ttcgttgatt caaatctgct aaatattcga caacatcgtc tggtagacag
     1861 tcaggacgcg taccaataga taatcccaca acacccggtt ctttaagtac aggttcgaat
     1921 ttttctttta atacttcaac cggtgcatgt gtatttgtaa atgcctgaaa ataagcaata
     1981 tattttcctt cgtgccattt ctcatgcatc ttttctttaa tttctttaaa ttgtactgcg
     2041 attgaatctg cacgattacc tgcaaagtct ccgctacctg cagcagaaca aaatgtacat
     2101 ccaccatgtg ctacagtgcc atcgcggtta ggacagtcaa acccgccatc caatgcaact
     2161 ttaaatattt tttgtccaaa tttatttttt aaatggtaat tccatgtgtg ataacgtttg
     2221 ttttcaaaag cgtattggaa atgattgccc atatgtcatt ttcctttcta taaaaaaaga
     2281 gttctaagta cagattttaa catattttaa tgttatagtg tttattatag tttgacaaaa
     2341 aagagagagg aactatgaaa tatgaatata cctaaatcag tctggtggct agtaattggc
     2401 atggcgttaa atattactgg ttccagtttt ttgtggcctt taaatacaat ttatatgaaa
     2461 caagaacttg gaaaaagttt aactgttgct ggtttagtgc taatgataaa ttcatttggc
     2521 atggttattg gaaacttatt aggtggttca ctatttgata aattaggtgg atacaagacg
     2581 attttaattg gaactttcac ttgtctttgt agtacaacgc tacttaattt ctttcacggg
     2641 tggccttggt atgctgtatg gcttgtaatg ttagggtttg gtggcggaat gattattcct
     2701 gcgatatacg ctatggctgg agcagtgtgg ccaaatggcg gaagacaaac gtttaatgcg
     2761 atatacttag cgcaaaatat tggtgtggct gtcggtgctg caatgggcgg ctttgtcgca
     2821 gaatttagct ttaactatat ctttttagcc aatcttatta tgtatgttgt gtttgcgctt
     2881 gtcgcggtaa cgcaatttaa tattgaaatt aatgcgaaag ttaaatatcc aactcattta
     2941 gatattactg gtaaaaagaa taaagcaaga tttatttcat tagtactaat ttgtgcaatg
     3001 tttgcaattt gttgggttgc atatattcaa tgggagtcta caatcgcttc atttacacaa
     3061 tctattaata tttcaatggc acaatatagt gttttatgga caattaacgg aataatgatt
     3121 ttagtagcac aaccattaat taaaccgatt ctctatctgt taaaaggaaa cttaaagaag
     3181 caaatgtttg tcggcatcat catttttatg ttgtcgttct ttgtcacgag ttttgccgaa
     3241 aactttacaa tatttgttgt cggtatgatt attttaactt ttggagaaat gtttgtatgg
     3301 ccagcagttc caactatagc caatcagtta gcgccagatg gtaagcaagg acagtaccaa
     3361 ggttttgtga attcagctgc tacagtagga aaagcatttg gtccatttct tggtggtgta
     3421 ttagttgatg cgtttaatat gcgcatgatg tttatcggta tgatgctact acttgtattt
     3481 gcattaatat tattaatggt tttcaaggag aataatacgc aacctaaaaa aatagatgca
     3541 taatgagtaa atagaattaa cgttatagac ttgaaataaa tgtcgttata acataatatt
     3601 aatttgtata atttaatttc gtttggagct tttctacaga aagctagtga tgctgagagc
     3661 tagtgttaag gactaaatgt aaatcgtatt aattttaaat tgaatgaatg acatctctta
     3721 ctattaaaat gagtgcacaa tttttgtgaa atagggtggt aacgcggcaa atgtcgtccc
     3781 tatgtaaata gaatagttag aggtgtcttt tttattgaat aggaggaaat gtgttgaatt
     3841 ataaccacaa tcaaattgaa aagaaatggc aagactattg ggacgaaaat aaaacattta
     3901 aaacaaatga taacttaggt caaaagaaat tttatgcttt agacatgttt ccatatccat
     3961 caggtgctgg tttacatgtt ggacatcctg agggctatac agcaacagat atcatttcaa
     4021 gatataaaag aatgcaagga tataatgtat tacatccgat ggggtgggat gcattcggat
     4081 taccagcaga gcaatatgct ttagacactg gcaacgaccc acgtgaattt acaaagaaaa
     4141 atatccaaac ttttaaacga caaattaaag aattagggtt cagttatgat tgggatcgtg
     4201 aagttaatac aacagatcca gaatactata aatggacaca gtggattttc atacagttat
     4261 ataacaaagg tttagcatac gttgatgaag ttgcagttaa ctggtgtcca gcattaggca
     4321 ctgttttatc taacgaagaa gtgattgatg gtgtctctga acgtggtgga catccagttt
     4381 atcgtaagcc gatgaaacaa tgggtactta aaatcacaga atatgcagat caattattag
     4441 cagatttaga tgatttagat tggcctgagt ctttaaaaga tatgcagcgc aattggattg
     4501 gacgttctga aggggccaaa gtttcatttg atgtagataa tacggaagga aaagtagaag
     4561 tatttacgac tagaccagat acaatctatg gtgcatcatt cttagtctta agtcctgaac
     4621 atgcattagt taattcaatt acaacagatg aatataaaga aaaagtaaaa gcttatcaaa
     4681 cagaagcttc taaaaagtca gatttagaac gtacagattt agcaaaagat aaatcaggtg
     4741 tatttactgg tgcatatgca actaatcctt tatctggtga aaaagtacaa atttggattg
     4801 ctgattatgt attatcaaca tatggtactg gagcaattat ggcagtacca gcgcatgatg
     4861 acagagatta tgaatttgct aaaaagtttg atttgccaat cattgaagtc atcgaaggtg
     4921 gaaatgttga agaagcagca tacactggtg aaggtaaaca tattaattct ggtgaacttg
     4981 atggtttaga aaatgaagcg gcaattacta aagctattca attattagag caaaaaggtg
     5041 ctggcgaaaa gaaagttaat tacaaattaa gagattggtt attcagtcgt cagcgttatt
     5101 ggggcgaacc aattcctgtc attcattggg aagatggaac aatgacaact gttcctgaag
     5161 aagagctacc attgttgtta cctgaaacag atgaaatcaa gccatcaggg actggtgagt
     5221 ctccactagc taatattgat tcatttgtaa atgttgtaga tgaaaaaaca ggtatgaaag
     5281 gacgtcgtga aacaaataca atgccacaat gggcaggtag ttgttggtat tatttacgtt
     5341 acatcgatcc taaaaatgaa aatatgttag cagatcctga aaaattaaaa cattggttac
     5401 ctgttgattt atatatcggt ggagtagaac atgcggttct tcacttatta tatgcaagat
     5461 tttggcataa agtcctttat gatttggcta tcgtacctac taaagaacct ttccaaaaat
     5521 tatttaacca aggtatgatt ttaggagaag gtaatgagaa gatgagtaaa tctaaaggaa
     5581 atgtaatcaa tcctgatgat atagtacagt ctcatggtgc agatactttg cgtctttacg
     5641 aaatgtttat gggaccttta gatgctgcaa ttgcatggag tgaaaaagga ttagatgggt
     5701 ctcgtcgatt cttagatcgc gtatggcgtt taatggtaaa tgaagatggg acattgagtt
     5761 caaaaattgt aactacaaat aataaatctt tagataaagt ttataaccaa actgttaaaa
     5821 aggtaacaga agactttgaa acattaggat ttaatactgc tattagtcaa ttaatggtat
     5881 ttattaatga gtgttataaa gttgatgaag tttataaacc ttacattgaa ggcttcgtta
     5941 aaatgttagc acctattgca ccacatatcg gtgaagaatt atggtcaaaa ttaggacatg
     6001 aagagtctat tacgtaccaa ccttggccaa cttatgacga agcactactt gtagatgatg
     6061 aagtagaaat cgttgttcaa gtgaatggta aattgagagc taaaattaaa attgctaaag
     6121 atacatcaaa agaagaaatg caagaaattg ccttatctaa tgacaatgtt aaagcgagta
     6181 ttgaaggtaa agacatcatg aaagtcatcg ctgttcctca aaaattagtc aatattgtag
     6241 ctaaataatg ttttaaggag gactttgaaa tgaagtcaat tactacagat gaattaaaaa
     6301 ataaactttt agaatctaaa ccagttcaaa ttgttgatgt tcgtactgat gaagaaacag
     6361 caatgggata tattcctaat gcaaagttaa ttccaatgga taccattccg gataatttaa
     6421 attcatttaa taaaaatgaa atatattata ttgtatgtgc tggtggagtt cgaagcgcta
     6481 aagttgtaga atatttagag gcaaatggca ttgatgccgt aaatgtcgaa ggcggcatgc
     6541 acgcatgggg cgatgaaggt ttggaaataa aaagtattta aagtagtgac ataatttaaa
     6601 ataatattac atttgtaatg acaccaagta acgtttcggt tgcttggtgt tttttggtat
     6661 gaattacttt ctgttacaaa acaatctaaa gcgttcttgt tatgttttat taagatttta
     6721 attacaaaac ggaaactaaa ttgtaataaa ataaaacttt attttataaa atgatgatga
     6781 taaaattgag
//
LOCUS       DAMQYT010000136         6768 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMQYT010000136 DAMQYT010000000
VERSION     DAMQYT010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.137"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            75..1325
                     /locus_tag="PGA33_002015"
     CDS             75..1325
                     /locus_tag="PGA33_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDG6473818.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            1508..2458
                     /locus_tag="PGA33_002016"
     CDS             1508..2458
                     /locus_tag="PGA33_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDG6473819.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            2607..4091
                     /locus_tag="PGA33_002017"
     CDS             2607..4091
                     /locus_tag="PGA33_002017"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDG6473820.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYDEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            4088..5047
                     /locus_tag="PGA33_002018"
     CDS             4088..5047
                     /locus_tag="PGA33_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDG6473821.1"
                     /translation="MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(5235..5342)
                     /locus_tag="PGA33_002019"
     CDS             complement(5235..5342)
                     /locus_tag="PGA33_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473822.1"
                     /translation="MHCIAGFLILLTKLNMTRELKNDVTSLCIPIPITT"
     gene            complement(5414..6130)
                     /locus_tag="PGA33_002020"
     CDS             complement(5414..6130)
                     /locus_tag="PGA33_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDG6473823.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(6149..>6768)
                     /locus_tag="PGA33_002021"
     CDS             complement(6149..>6768)
                     /locus_tag="PGA33_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDG6473824.1"
                     /translation="KIEAINNEMRKFRHDYVNILTTLSEYIREDDMPGLRDYFNKNIV
                     PMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNFPISIEIPDEVSSINLNMI
                     DLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQES
                     FSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN"
BASE COUNT         2339 a          944 c         1236 g         2249 t
ORIGIN      
        1 gatgtaataa aacttaacgc atttttgctt tttataaatt gtcagattat tatgaaaaaa
       61 agggagtggt aagtatgaat cttaacgata cgatatttat gtttttgtgt acattattag
      121 tttggttaat gacaccagga ttaagtttat tttatggtgg gttagttcaa tctaaaaatg
      181 cgcttaatac tgtcatgcaa agtatggcag caattgtgct tgttacattt gtatggataa
      241 cagttggttt tacaattagt tttgggaatg ggaatttatg gttcggaaat tgggaatata
      301 cttttcttaa tcatgtaggt tttgcgactc aagaagatat tagcccacat attcctttcg
      361 ctttgtttat gttatttcaa atgatgtttt gtacgattgc aatttctatt ttatctggtt
      421 caatcgctga gaaaatgaag tttattcctt atttattatt cgtagtaata tggactgctc
      481 ttgtatacag tccagtagca cattgggttt ggggcggcgg ttggattaac aaactcggtg
      541 tattagattt cgctggaggt acggttgttc atattacatc aggtgtttct ggtttagtat
      601 tagctattat gattggaaaa ggaaacaaac attctgaatc aacaccacat aatcttatca
      661 ttacgttgat tggcggtata ttcgtgtgga ttggttggta tggatttaat gtaggtagtg
      721 cttttacatt tgataatatt gcgatgcttg catttacaaa tactgtcatt tcagccagtg
      781 caggtgctat aggttggtta attttagaat atatttttaa aaagacgaca agtttacttg
      841 gacttttact cggtgcatta gcaggattag ttgtcattac tcctgcagca ggatatgtaa
      901 catatcttag tgcaacaata atggctttaa taggaggtat ctgttgttat attgtcatta
      961 attacatcaa ggtaaaacta aaatatcatg atgcattaga tgcatttggt attcatggtg
     1021 ttggtggtat tattggtgct gttttaacag cagttttcca aagtaaaaaa gccaatcctg
     1081 acattgagaa tggctttatt tatactggtg acatacatat tatacttgta caaatattat
     1141 gtgtaacagc agttgtaatt tttagtatag tcatgacgtt tattattgcg aaagtaatta
     1201 aattaattac accattatct gttacggaac aagaaacgaa tataggatta gacaagattg
     1261 ttcacggtga acatgcttac tttgaaggtg agctaaatag attcaataaa catattcgat
     1321 attagaatat atttacatag aatattcatt gtcctgacat ttaactaaag gttgatgttg
     1381 ggacattttg ttatacaaaa gttttatttt gaaatctttt tatgaaagaa gcagaaatat
     1441 tatttaaagc ggttacacat atgctaaaat aaggctaagt gtcacaaata atgataggtg
     1501 aataagtatg aaaaatatat ctgatattgc caaattggca ggcgtttcaa aaagtacagt
     1561 atctagattt ttaaataatg gatctgtcag taaaaaaaca agtgaaaaat taacaagaat
     1621 tatagcagaa catgactatc aaccgaatca atttgctcaa agtttaagag cgagacaaac
     1681 acaccttatt ggtgccatta tacctagaat gaattcatat gcagtagatg aaacaatcaa
     1741 aggattggca aaacaatgcc aaaaatatga atcacaatta attttaaatt acacaggttt
     1801 aaatatcgaa gcagaaatac aagcgcttga aacattagca cgcagtaaag tagatggtat
     1861 tgttttaatg gctacagaca taacagagag acatattgaa gtcattaata aaatgaatgt
     1921 accaatcgtt attgttggtc aacaacatga acaacttcat agtattgtgc atgatgatta
     1981 taaagcaggt caaattatag gcgaatggat tggtcaacag ggatatcaac aagttgaagt
     2041 gtttagtgta agtgaaaaag atattgcagt tggtatacat agaaaacgtg gtttacttga
     2101 ccagttagct aaataccaaa ttaaacctaa tatacatgaa acaaatttta cttatgtgga
     2161 agcacaaaaa gatgttgcaa atgttttgga aaatgtggag caagtagatg cggttgttgg
     2221 agcaactgat acgattgcat tagctgccta taaatattat tctgataaaa aagatgttat
     2281 gaaaccacat caaatatatg gttttggtgg tgacccaatg acacaattag tgtctccatc
     2341 gataaaaaca attcattata attattttga agctggccaa tgcgcgatgg aagagataca
     2401 acagatgctt aaaaagcaag atatgccata tagcgtcaca gtagatgtta atatttagac
     2461 gctgtatttt ttaaaataaa tgtggaaccg ataccatata actataaatg gataggttaa
     2521 aagttaaaga acgtaggtaa aatttgctat aatagaatat aaattgttat tagcataaat
     2581 tataaaagga ggactgggta aatattatga ccgaatggac tagagaagaa cgttatcaac
     2641 gaatcgagga cgttgatact gagtatttta aaacattaaa acaacaagtt gatcaatcaa
     2701 aatttcgtca acaatttcat atacaaccag aaacaggctt attaaatgac cccaacggac
     2761 ttatttttta taaagggaag tattatgttt cacatcaatg gttcccatta ggcgcagtac
     2821 atggcttaaa gtattggtat aactacacga gtgatgactt aataaacttt aaagctgaag
     2881 ggccaatttt aaatccagat actaaatatg acagccatgg tgtatatagc ggtagcgctt
     2941 ttgaatataa cgggcattta tattatatgt acacaggaaa tcatcgagat aatcattggc
     3001 aacgacatgc gagtcagatg atcgcacgat tgaaagaaga cggttcagtt gaaaagtttc
     3061 caaagccagt aattagccaa caaccagaag gatatacgag tcattttaga gatcctaaag
     3121 tttttaaata tgatgagaaa tattatgcaa tcattggtgc acaaaataat gatcagcaag
     3181 gtcgattatt actttataat actgaagata taattaattg gcattattta ggtgaaataa
     3241 atacagagtt ggatgatttt ggatatatgt gggaatgccc agattacttt aatgtagata
     3301 atcaagatgt catacttatt tgtccacaag gtattgaacc aaaaggcgat cagttcaaaa
     3361 atatttatca aagtggttat atacttggaa agtttgatat tgaaaagtta acatatgaac
     3421 atgaaaattt tgtcgagctt gataatggtt ttgatttcta tgcacctcaa acatttttag
     3481 atgaaaaagg ccgacgagta ctaattggat ggatggggtt accggaaatc gaatatccta
     3541 ctgataatga aggatgggcc cattgcttaa caattcctcg tgtattaaat gtagaaaatg
     3601 gtcaacttaa gcaacgtcca tatccagcgt tggaaaaatt acgtcacaat aaagagacag
     3661 cattaggcta cgcaaataaa tttactcgaa aattacatcc gtatgaaggt aaacagtatg
     3721 aattaatcat agatattttg gataatgatg ctaccgaagt gtactttgaa ttacgtacat
     3781 ctaagacttc ttcaacatta attgcttata acaagcgtga aaataaaata acattagatc
     3841 gcagcgacag tggtttattg ccgacaaatg ttgaaggtac gacgcgtagt acgatattag
     3901 acacgccatt aaagcaatta caaatttttg ttgatacatc tagtatagaa attttctgta
     3961 atgatggaga acgtgttttg acatctcgaa ttttcccgac agaagacgca ctaggtatta
     4021 aaacatcaac agaatcaggg caagtatatt tacaatttac taaatatgat ttaaaggatg
     4081 aacataaatg agacgtctat tttcaatagg agaagcgtta attgatttta ttccaaatgt
     4141 tacaaatgcg aatttaaaag atgttcaaac atttacgaag caaattggtg gcgcgccatg
     4201 caatgtagct tgtacagttc aaaagttagg acaacaagcg tatatgatta cacaattagg
     4261 taatgatgca tttggagata gcattattga aacgatatca agtattggtg tagatgtatc
     4321 aaaagtgtat cgcacgaatg aagcaaacac tgcgttagct tttgtgagtt tgactgaagc
     4381 gggcgagcga gatttttctt tttatcgtaa gccctctgct gatatgttat ttgaaccaag
     4441 ttttgtgaat gatattgatg tgaatgaaaa tgatgtagta catttttgtt ctgttgattt
     4501 ggtcgatagt ccgatgagag atgcacatta tcaattgatt accaaaacat taaatgcaaa
     4561 tggtactgtc gtatttgatc ctaatgtaag attgccatta tgggataacg ctgaagattt
     4621 aagacaaact attcatacct ttttgccatt ggcgcatatt gtaaaagttt cagatgaaga
     4681 attagaattt attacaggta ttcatgatga aaatgaagcc attcaatcat tatttacagg
     4741 taatgttact gtagtcattt atacgaaggg agcagatggt gctgccgtat atttgaaaaa
     4801 tggtattaat cattatcata gtggctataa ggtaaaacct gttgatacaa ctggggcagg
     4861 ggatgcattt attggtgcag ttattagcag gattttagca accgatgtgt taaatttaac
     4921 tcagttattt gaaaatgaag gtgaggaaat tttagcgttt agcaatcgcg ttgctgcaat
     4981 agtgacaacg aagtatggtg ctattaatag tttgccaaca ttacaagagg ttgaacaagc
     5041 attttaaata attcatttat aaaacagtgt aggaaacaag ttgaatcttg tgctacactg
     5101 ttttttaaaa caaaataaga ttcacggagt aggaaattat agcattttat ttatggaaac
     5161 tgtcaatttc aaatttagta tattttgtta aaaattgcgc cataggattg tagagtgaaa
     5221 tagaaattga taaattatgt agtaatagga ataggtatac aaagagacgt tacatcattt
     5281 tttaattcac gcgtcatatt taattttgtt aataaaatta aaaagccagc tatacagtgc
     5341 atttgctagt tatcttgtta aaatccaaca agatttacaa ttgaatacgc cgttaactga
     5401 ctttattatc ttattatatt tttttaacgt ttctcaccga tgcatagcag tgttctttat
     5461 ttttaaaata gacaattcgc tctttcgaat ctatagattc aatattatgg cgattgacga
     5521 caaagctatt atgacatcta aagaaacgat catctaattg actcagttct tttaaattac
     5581 cataaaattc aatttgacgg ttatctaaat gggcaatgag tctgtgagat tttgttgatg
     5641 attcaaaaaa cataatatca tcatattgaa catacactga attactgcca cgttttaatt
     5701 caatcgtttc aacgctatta tctttagaca acaattgtaa gcgtgtatgt gcagtttcta
     5761 aacagtctat aattcgagtt cttaattcag ctggatcatc tttaaaaata aaatccatcg
     5821 ctgcaacttt gtagacaaat gttaaatagg taagttcact gtgactcgta acgaaaataa
     5881 tgttaccaac tgggtcatgc ttacgaattt cactgcctaa tttgatacca ttaatatcag
     5941 ttgaaagttg aatatctaaa aagtaacagc ctatgtcatt catattttta gcttgctcaa
     6001 gcacctcata aggattatca gttgcgaggg caatttccat aggcttttct tctatcatta
     6061 tataattttt aataatggta accatgtttt ctctttgttt tggatcgtct tcgcaaatga
     6121 aaattttcat acattcacat ccttatggct agttgttaat aatttcaact ttttgaataa
     6181 agaaaccatt ttcgataatt gtatctaata agacattgtc tgcattatca gcaatttctt
     6241 ttaaagttga tagacctaaa ccacgacctt cacctttagt agaaaaactt tcttggaaca
     6301 attcatgaat gcgtggtata tcatcagcgc atttattcat aacaataaac gttactgaat
     6361 tttcactttc aataaatgca acgcgaatga tagggtcatc aatttcagtt gatgcctcaa
     6421 ttgcattatc aagaataata ccaatactgc gacttaaatc gatcatattc aagttaatgc
     6481 tacttacttc atcgggtatt tcgatactaa tcggaaaatt catttcttgt gcacgtaaaa
     6541 ttttcgcagt aattaagcct ttaatttcac gtactttaag attctcgata ccatttaatt
     6601 ttatagcatt catttgtaaa ttgtctttca taggtacaat atttttattg aaataatcac
     6661 gtaggccagg catgtcatct tctcgaatgt attctgaaag tgtcgttaag atattgacat
     6721 aatcatgacg gaacttgcgc atttcgttgt tgatagcttc aatcttca
//
LOCUS       DAMQYT010000137         6644 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMQYT010000137 DAMQYT010000000
VERSION     DAMQYT010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.138"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..1763)
                     /locus_tag="PGA33_002022"
     CDS             complement(<1..1763)
                     /locus_tag="PGA33_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDG6473825.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLR"
     gene            complement(1787..2758)
                     /locus_tag="PGA33_002023"
     CDS             complement(1787..2758)
                     /locus_tag="PGA33_002023"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDG6473826.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRATSIQLHDKNKLDRYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            complement(2745..3947)
                     /locus_tag="PGA33_002024"
     CDS             complement(2745..3947)
                     /locus_tag="PGA33_002024"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDG6473827.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            complement(3952..5094)
                     /gene="bshA"
                     /locus_tag="PGA33_002025"
     CDS             complement(3952..5094)
                     /gene="bshA"
                     /locus_tag="PGA33_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HDG6473828.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            complement(5335..5652)
                     /locus_tag="PGA33_002026"
     CDS             complement(5335..5652)
                     /locus_tag="PGA33_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDG6473829.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(5989..6642)
                     /locus_tag="PGA33_002027"
     CDS             complement(5989..6642)
                     /locus_tag="PGA33_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014533305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDG6473830.1"
                     /translation="MLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEILHANGIYDV
                     EVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDHQGYWFLRFR
                     AALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLFSIVTLPVEF
                     DASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRIILIARSSD"
BASE COUNT         2086 a         1233 c          874 g         2451 t
ORIGIN      
        1 ttccgtaatg atttaactgt tttatgacta atgccattga atatttctag cgttttattt
       61 aacttatcga taatcgcatg taaatccttc aaaatgtctt ttgtttcaaa agtaaataca
      121 ttatggaagc gatgaatatc atcatcataa acatcagaat cattgataat cgtaaatatc
      181 gttgagaaca attgctcatt taactcatga atctcattca tactagcctt caagccaaaa
      241 atatcaattg gtgcaatatc taatttttcc aaaattcgct gcttttccag ttgatcaatt
      301 gcctttaaca atttttcatt ttcgttttta ccaatcaaac caagctgata tttaatatca
      361 gcataactca actcatttgt cacttgattt aaggcatagt ctggtaagcg atgtgcttca
      421 tccactatac aatcatcaaa caattgatat attgaatttt caacatcaga atgaattaaa
      481 tgtgcatgat ttgtaatacc aatttgaatg ttctgtgcat ttcgcttaat aaaattataa
      541 taatgaacat cgtgacgtgc cggtacatat gtttcaattt tctggtcaaa atacatcttt
      601 tgaccacctt ttaaatttaa ttcctgtata tctccggacg gcgtctctgt aatccaaatc
      661 agcaattgca ttttcaagat attcacttcg taattacttg tgtcatcttt taaaatttga
      721 ctaataagcc ccaatgaaat gtaatcactt ttacttttaa tcaatagtgc attaatttta
      781 aaattcaacg cttcattcat tgctggaata tctttttcta acaattgact ttgcagtaat
      841 ttagtattgg tagaaatcat gacatgcttc ccagtttcaa tattatacat caaggccgca
      901 agtaaatatg ctaatgattt accactgcct agtgatgctt caatcattgc tttttcacta
      961 tgcatgagct gatctaatat agtttccgct aaatataatt gttgcggtcg atatgttaag
     1021 ccaagttgat ctacagcttt gctatataaa gacttcaagc tgccattata atttgttgtc
     1081 ggctttttaa aatcaacttg cttacgatag ataatctgtt cgaacttttc gtacgattta
     1141 tccaatggct ttgcatcata ttgcctaacc atctcaaaga aaatatcata caaatcgtat
     1201 ttcaactgtt tacttaaata atataattgc ttcaaagtat ctaacggtaa cttttcaaat
     1261 ttttcaaaag ctaatatcat caatttagca gtagtagcgg catcttcgtc agctcgatgg
     1321 gcatttgcta aggtaatacc atgtgcctct gctaattcac ttaattgata gcttttatct
     1381 gtaggaaaag ctattttaaa gatttctagt gtatctataa cttttttggg acgatattga
     1441 atattacaat ctttaaatgc ctttttaata aaattcaaat caaaatctac attatgagct
     1501 acaaaaatgc aatcttttat cttatcgtag atttcttgtg caacttgatt aaaatatggc
     1561 gcttgttgta gcatattttc ttcaatggat gttaacgctt gaatgaacgg cggaatctct
     1621 aaatttgttc taatcataga atgatatgta tcaataattt ggttattgcg cacaaacgtt
     1681 ataccaattt gaatgatatc gtcaaaatct aattggttgc ctgttgtttc caaatccaca
     1741 acggcatagg ttgccatacc catagctatc tctccttgct ttagtgttaa aaatctatat
     1801 ctgcactaat taaacggtgt gattcacccg cttcatctct aacaattaga tagccatcgt
     1861 aatctaaatc aattgcttgt cctttaaact gtttatcatt ttctgtaaat agcaacgttc
     1921 tattccaaat attagaagct gcagtatatt cttcacgaat ttcagaaaaa ggtaacgtta
     1981 aaaattgatt atatcttttt tcaatttctt gaagtaatat ctctaaaaat tgatatctat
     2041 ctaatttatt tttatcatgt aattgtatac ttgttgctct atgtctaata ctttcatcaa
     2101 agttttctag ttgttgcgtc aaattaatac ctataccaca tattattgct tctataccat
     2161 cattattagc aaccatttca gttaagaaac cacacacttt accattatca atatatatat
     2221 cattcggcca tttcactttg acttcatctt gactaaaatg ttgaatcgca tctcttatcc
     2281 ctaatgcaat aaataaatta aatttagata tcattgagaa tgcaacgtta ggtcttaaca
     2341 cgacagacat ccaaagtcct tgcccttttg aagaactcca atgtctatta aatcgcccac
     2401 gacctttcgt ttgttcatca ctcaagataa aaaatgaaga ttgatttcca acaagtgact
     2461 ttttcgcagc aagttgtgta gaatctattg aatcgtatac ttcactaaaa tcaaacaaag
     2521 cagaactttt tgtatattgg tctattatac cttgatacca aatatctggg agctgttgta
     2581 ataaatgccc tttatgattt actgaatcta ttttacatcc ctctaacttt aattggtcaa
     2641 tcactttttt tactgcagtg cgtgaaatat taagtgattc cgcaatgctt tgtccagata
     2701 tataattcgg tttattttta tagagtaatt gaagtacatc ttgactatat tttgacatga
     2761 ttatccaccc atttcaaaat ttcagtttct tcgttgctta ctttacctgt tacaatcgct
     2821 atctcaattt gtcttagcac atcttttaac cacggaccac ttttggcatt taaatgtgcc
     2881 ataagtacac cgccattaac catcatgtct tttctattat gcataggtaa acgatgtaat
     2941 gtttcatcaa tcgtttgaag gttaacgatt aatggttcat gtccttgaat atcatttgcc
     3001 tttaacacgt ctgctgcaac cattacattt ttaatgagat gcgtatcata atcataaaca
     3061 aacattttta attgttcttt tgtaataata cttggtaatg cattcataat ttgaatatat
     3121 tgattgatat cttttacttg tcggttactt agctttaaag gcttcaatga gtaattaata
     3181 tcaaatttaa ctgatactat agcaatcaac aattctaaat caattgcttc agttacatta
     3241 atttgattca tatcaagatg ttcgaaatac ggcatataat taaatgcttt cagcgatttt
     3301 aaatgattaa aactcttttc aacattaata cctcgcatta atttagttag ttcaatcact
     3361 atacgctcaa ttgataaaaa tttaatatct gccatttgta tacgcatcgc ttcgaatgtt
     3421 tccgttgcaa tatcaaatga taattgtgac tggaacctta aacatcgaat catacgtaaa
     3481 gcatcttctt ggaatcgttc ctcagctata cctacagttc ttattattcg attattaata
     3541 tcttgttgac catcaaaata atcatacaat ttgtatgctg tatccattgc tatcgcattc
     3601 atcgtgaaat ctcgtcgttg caaatcttcg tataaatcac gaacaaatgt aacaccactt
     3661 ggtctacggt gatcgacata atcttcttca gcccggaatg ttgtcacttc ataattttca
     3721 tcattaaaaa ctacatttat cgtgccatgt tctttaccta caggtatcgt atgactaaag
     3781 atagattcta tttcatccgg cgttgcactt gttgtgatat ctatatcatg aatatttctt
     3841 cccatgacat aatctcttac agagccacct acataatatg cttcaaaacc attgtcttga
     3901 atttgttcta atataggcct tgcctgttca aataatgatt tatccatatt attactcgcc
     3961 tttacttttg ttatgctcat ttagcatttt ttgataataa tactcatatt gatctgtaat
     4021 aagttctgat ccaaaacgtt cagcaatatc tgctagcatg tttttctgaa gtttgttgta
     4081 taacacctta tcttcaagta atcggatagc atagtcactc gctgaatcac aatcacccac
     4141 atctacgaca aatccagttt caccatgttt aataacctct ttaattccac cggcatttga
     4201 accaattgga acgacgcctg ttttcatagc ctcaagtaaa gttagtccaa agctttcttt
     4261 ttcacttaat aataatacta agtcagataa ttggtaaaat tcacttacgc aatcttgttt
     4321 ccctaaaaat aaaacatcct cttctacgtt taactctttc gtcaattgac gcattggcac
     4381 taattcagga ccatctccaa gtaaaattaa tttactaggt atcttttcac gtacttttgc
     4441 aaatgtttct ataatagtat ctatgcgttt tacttgtcta aaattcgata catgtattaa
     4501 cactttttca tctggtgcta taccaaattg tgattttaat gctgtgttat gtttagttgg
     4561 aaactcattt tcacgtacaa aattataaat cggtataatt tctttgttag tttcaataat
     4621 ttcatgtgtt tcttgtgcta aagatttact cacacttgtc acaatatcac ttttttcaat
     4681 gccaaattta attgcacctt ggagtgaatg atcatagccc aaaacagtaa tatcagtacc
     4741 gtgtagcgtt gtcataattt ttatatcttt acctgacatc tcacgagcta aaatcccaca
     4801 aattgcatga ggtacagcat agtgcatatg caacaaatca agatcatatt ctttaataac
     4861 ttcagcgatt ttagtactta acgtaatatc atacggtgga tactgaaata ctgcatattg
     4921 attcacttca acttgatgaa aaatcatatt cggtaatggt tttcttattc taaacgggat
     4981 atttgaagtg ataaaatgta cttcgtgacc tcgctctgct aatttaattc ctaattctgt
     5041 ggcaataatt ccagaaccac ccatggacgg gtaacatgtt atacctatct tcattcgctt
     5101 gcccatcctt tctttctatt tctctctatt attctcgatg cgtagataat tgtttaaatt
     5161 taagtttata gtaatgttga gtttataatt tcatatatct aaaaacaggt gttgtatata
     5221 taatcattca tctagttata cttactttaa aaataatata atttcatgcg atgcaattca
     5281 ttgatggatg tttttaatct taatcaaatc caaataaagc atatattttt aaattcactt
     5341 tctttcgaat cgatttttat ctcttgtatt aaacttttcc attgtttcat taaagctctc
     5401 tgtcatatct attcccattg aattcgctaa acataacaac acaaataaat tatcacctaa
     5461 ttctgcttta atcgtatttg cttcctctga atctttcttc tttttttcac cataggtatg
     5521 atttatttca cgtgcaagtt cgcccacttc ttcagtcaat ctagctaagt tagctaatgg
     5581 tgaaaaatat cctgttttaa attgtccaat atattcatca acttcacgtt gcatttctac
     5641 cattgatttc atttctacgt tctccttata ttgcatttct aatatagtat atatcaattt
     5701 gaagtctcat gcatgtttag tgcaatctac tgactctagc taaactagat gacaagttgt
     5761 ttattttaga agtttaataa ttcagtttat ataaatgtaa tgcattccta actaaattaa
     5821 atcaattgaa attgggatta taactttatg atacgtacca ctacaataaa ataatatagt
     5881 gaataatcta ccattagaaa aataagcaca aaaaaactag caaccacaca aaaatgtgat
     5941 tagctagtta ataagtgtct aatttaagtt aattgttaat ctataagatt aatcacttga
     6001 acgcgcaatc aaaataatac gtacaagctc tgctacagcg actgcagttg ctgcaacata
     6061 agtcattgct gctgcagata atactttacg cgcatgcttg tattcttttt catttacaat
     6121 gttcaatgcc gtaatttgtt tcatcgctct tgaactcgca tcaaactcaa ctggtaacgt
     6181 aacaattgag aataataccg ctaatgacat taaaccagca ccaatccata aagcagttga
     6241 accaaatgca ctacctatcg ctgttaagat aatacctaac atgatgatca tataacttaa
     6301 tgaactccct aggtttgcaa caggtactaa tgctgctctg aatcttaaga accaatatcc
     6361 ttggtgatct tgaatggcat gaccaacttc gtgggctgca attgcagttc cagcaactga
     6421 tggtctgtca tagtttgcag gagatagtga aacaactttc tttttaggat cgtaatgatc
     6481 tgttaagaat ccttcacctt taacaacttc gacatcataa ataccgtttg catgtaaaat
     6541 ttctaatgca acttcacgac ccgttttacc actagttgat ctaacttgtg aatatttctc
     6601 atagttagat ttaactttgt gttgtgccca taaaggaagc acca
//
LOCUS       DAMQYT010000138         6528 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMQYT010000138 DAMQYT010000000
VERSION     DAMQYT010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.140"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            77..310
                     /locus_tag="PGA33_002028"
     CDS             77..310
                     /locus_tag="PGA33_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473831.1"
                     /translation="MFNILAELDFEDEAFLILDEVTMTLSDVMKETQHVYRYSVIDEK
                     GEHKHTTDRKGHVIGMLEWALDYIVGNIEVEEL"
     gene            311..559
                     /locus_tag="PGA33_002029"
     CDS             311..559
                     /locus_tag="PGA33_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HDG6473832.1"
                     /translation="MNWEIKNLMCDLKILKEKFEDLKDNHGSHFEDLYLHEPNHTLNK
                     DDAIKEGFSYHERRIHNDQMFDALEKASSFADQSQDNA"
     gene            651..3023
                     /locus_tag="PGA33_002030"
     CDS             651..3023
                     /locus_tag="PGA33_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDG6473833.1"
                     /translation="MAIKKKDRIIGVKELEIPQELKLVPNWVLWRAEWNEKQQNFGKV
                     PYSINGYRASTTNKKTWCDFESVSIEYEVDEQYSGIGFVLSDGNNFVCLDIDNAIDKK
                     GQINSELALKMMQLTYCEKSPSGTGLHCFFKGKLPDNRKKKRTDLDIELYDSARFMTV
                     TGCTIGQSDICDNQEVLNTLVDEYFKENLPANEVVREESNTNIQLSDEDIINIMMKSK
                     QKDKIKDLLQGTYESYFESSSEAVQSLLHYLAFYTGKNKQQMERIFLNYNNLTDKWES
                     KRGNTTWGQLELDKAIKNQKTIYTKSIDEFNVIPQGSKDVKQLLNQLGHEERTKMEEN
                     WIEEGKRGRKPTTISPIKCAYILNEHLTFILFDDEENTKLAMYQFDEGIYTQNTTIIK
                     RVISYLEPKHNSNKADEVIYHLTNMVDIKEKTNSPYLIPVKNGVFNRKTKQLESFTPD
                     YIFTSKIDTSYVRQDIVPEINGWNIDRWIEEIACNDNQVVKLLWQVINDSMNGNYTRK
                     KAIFFVGDGNNGKGTFQELLSNVIGYSNIASLKVNEFDERFKLSVLEGKTAVIGDDVP
                     VGVYVDDSSNFKSVVTGDPVLVEFKNKPLYRATFKCTVIQSTNGMPKFKDKTGGTLRR
                     LLIVPFNANFNGIKENFKIKEDYIKNQQVLEYVLYKAINLDFETFDIPDASKKMLEVF
                     KEDNDPVYGFKVNMFDQWTIRKVPKYIVYAFYKEYCDENGYNALSSNKFYKQFEHYLE
                     NYWKTDAQRRYDNEELAKRIYNFNDNRNYIEPIESGKNYKSYEKVKLKAI"
     gene            3694..4038
                     /locus_tag="PGA33_002031"
     CDS             3694..4038
                     /locus_tag="PGA33_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDG6473834.1"
                     /translation="MNRNRMKQIILEYIKNNDSTSFVEIENVFEEQGFKYKGNGAYTS
                     GNHKNIIFWMGWNEEAFNIVADLKRDGLIEMQICPPMYYLIDGKGLRLPIVKNKNIKI
                     DHWLPVTFSLVN"
     gene            4263..4469
                     /locus_tag="PGA33_002032"
     CDS             4263..4469
                     /locus_tag="PGA33_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473835.1"
                     /translation="MLDHTNESVKRVFKYRDKQELLNKYHELLDKYFLHNISFYEEDK
                     NNERIYGLVVHTKNKLGDEDYERV"
     gene            4456..5514
                     /locus_tag="PGA33_002033"
     CDS             4456..5514
                     /locus_tag="PGA33_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HDG6473836.1"
                     /translation="MKEFKEQFGYQLSNFDDMDIKGYANLYQKDIGKDVSMIEQGLKQ
                     LSITETEVLLPEQINKKLLGVLNMNEVNQSSNTWVGTLTKQLVSSENNFNIQELPTAR
                     KEVFKELLVNCELPQSLREVITITDDEHVESIPSLTYIKDKLATNGIELSLNGSSKYF
                     DRREGHIYTEVSDSVVHGSDRTLDDLLKEIFINECVAYETTLLLDKNNSSGLIDKGNQ
                     DLSLYNQGIKEVSNTSIYDGIKQAMKDIPQTFRRNVSIVMNTEHHDKLIKELAQMGLG
                     GLAGDLSNLFNVTNVVVTDDAHDVFVGDFGHAIYAKYEPIMYNKKKQALKGIYQFALN
                     YVFDIKIIPELLRIVNIK"
     gene            5698..6189
                     /locus_tag="PGA33_002034"
     CDS             5698..6189
                     /locus_tag="PGA33_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HDG6473837.1"
                     /translation="MVSDFESNDKITEVELLMHYNPKVINSKIKAMRSQIESLYHLNM
                     NHVITNDNDMLVSVSYPLDKLVLYIIEEKDKLEYYMKTAQDRLNLFKNIIKNYSENEQ
                     QDVMRYMLSSGKVKNEGVIERLKVDIYKVESRKRQERQNQREELHRIEFNKHLEQVKK
                     ELS"
     gene            6189..6341
                     /locus_tag="PGA33_002035"
                     /pseudo
     CDS             6189..6341
                     /locus_tag="PGA33_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
BASE COUNT         2581 a          782 c         1218 g         1947 t
ORIGIN      
        1 gtttcaatcc gataaggact atcattatca ggataaagca ttatatgaat atgctttaaa
       61 gttagttcat aatggattgt tcaatattct tgctgaatta gattttgaag atgaagcatt
      121 tttaattctt gatgaagtaa caatgacgct aagtgatgtc atgaaagaaa cacaacacgt
      181 ttaccgttat agtgtcatag acgaaaaagg tgaacataaa catacaacag atcgcaaagg
      241 acacgtgatt ggaatgttag agtgggcatt agattacatt gtgggaaata ttgaagtgga
      301 ggaattataa atgaattggg aaattaaaaa tttaatgtgt gatttaaaga tattgaaaga
      361 aaagtttgag gatttaaagg ataatcatgg ctcgcatttt gaagatttat atctacatga
      421 gccaaatcat accttaaata aagacgatgc tattaaagaa ggattttcat atcatgagag
      481 acgtattcac aatgatcaaa tgtttgatgc attagaaaaa gcgtcatctt tcgccgacca
      541 aagccaagat aacgcataaa aaaataaaaa tttataaaat cataccatta atattttaac
      601 attagttgca ttgaaaatac agagaaatca gattagaaag gaggcattct gtggcaatta
      661 agaaaaaaga tagaattatt ggagttaaag aattagaaat accgcaagaa ttaaaattag
      721 tgcccaattg ggtattatgg cgagctgaat ggaacgagaa gcaacaaaat tttggaaaag
      781 taccatatag tattaatggt tacagagcta gtacaaccaa taaaaaaaca tggtgtgact
      841 ttgaaagtgt aagtattgaa tatgaagttg atgagcaata tagcggtata ggttttgtat
      901 taagtgatgg taataatttt gtttgcctag atattgataa tgcaattgat aaaaaaggac
      961 aaatcaattc tgaattagca ctaaaaatga tgcaactcac atattgtgaa aagtctccaa
     1021 gtggtacagg attacattgt ttctttaaag gtaaactacc agataaccgt aaaaagaaaa
     1081 gaacagattt agacatagag ttatatgatt cagcaaggtt tatgactgtt acaggatgca
     1141 caattggtca aagtgatatt tgtgataatc aagaagtatt aaatactcta gttgatgaat
     1201 attttaaaga gaatttgcca gcaaatgagg ttgtgagaga ggaatctaat accaatatac
     1261 aattatctga tgaagatatt ataaacatta tgatgaaatc taaacaaaaa gataaaatta
     1321 aagatctatt acaaggcaca tatgaatcat attttgagag ttcaagcgaa gcagtacaaa
     1381 gcttattaca ctacttagcg ttttacacag gtaaaaacaa acagcaaatg gagcgtatat
     1441 ttttaaacta caataatctt acggataaat gggaaagtaa acgaggtaat acgacttggg
     1501 gacagcttga gttagataaa gctataaaga atcaaaagac aatttatact aaatcgatag
     1561 atgaatttaa tgttatacca caggggagta aagatgttaa acagttgttg aatcaattgg
     1621 ggcatgaaga aagaacaaaa atggaagaaa attggattga agaaggaaaa cgagggcgaa
     1681 agcctacaac aattagccct ataaaatgtg catatatttt gaatgagcat ttaacattta
     1741 tactttttga tgatgaagaa aatactaagt tagctatgta tcaatttgat gaagggatat
     1801 atacacagaa cactacaatt ataaaacgag tgatttccta tttagagccc aaacataata
     1861 gcaacaaagc tgatgaagtt atttatcatt taaccaatat ggtagatata aaagagaaaa
     1921 ctaactcacc atacttaata ccagttaaaa atggtgtatt taaccgtaaa acgaagcaac
     1981 tagaatcatt tacacctgat tatatattta ccagtaaaat agatacatcg tatgtaaggc
     2041 aagatatagt acctgaaata aatggctgga atatagatcg gtggatagaa gagattgctt
     2101 gtaatgataa tcaggttgtt aaattattgt ggcaagtaat taatgactca atgaatggta
     2161 actacactcg taaaaaggct attttctttg ttggtgatgg aaataatggt aaagggacgt
     2221 ttcaagaatt attgtctaat gtaataggtt atagcaatat tgctagctta aaagtgaatg
     2281 agtttgatga acgttttaaa ttgagtgtgt tagagggcaa gacagcagta attggagacg
     2341 atgtaccagt tggtgtgtat gtcgatgatt cttcaaactt taaaagtgta gttactggcg
     2401 atccagtgtt ggttgagttt aaaaataaac ccttatatag agcgactttt aagtgtacag
     2461 ttattcaatc aacgaatgga atgcctaaat ttaaagacaa aacaggcggg actttaagaa
     2521 ggttattgat agtaccgttt aatgccaatt ttaatggcat taaagagaat tttaaaatca
     2581 aagaagacta tataaaaaat cagcaagtgc tagagtatgt gctttataaa gcaattaatt
     2641 tagattttga aacctttgac attcctgatg catctaaaaa aatgcttgaa gtatttaaag
     2701 aagataacga tccagtttat gggtttaaag taaatatgtt tgatcaatgg actattagaa
     2761 aagtgccgaa atatattgta tacgcatttt ataaagaata ttgtgatgaa aatggctata
     2821 atgcattgag ttcaaacaag ttttataaac aatttgaaca ttatttagag aattattgga
     2881 aaactgatgc acagcgaaga tatgacaatg aagaacttgc taagaggata tacaacttta
     2941 atgacaatag aaattacatt gaacctattg aaagtggaaa aaactataaa tcgtatgaaa
     3001 aggtgaagct aaaagcaata tagttgcaac cgaatgcaac cgaatgcaac cgaaaatttt
     3061 aaaacgggtt gcacctataa tcacagttat atcaagggtt tagggcactt atgcaactga
     3121 gcaactcaaa atctataact tattagtaga aaagtaaata accaaaatat aaataagaaa
     3181 tagtatcaaa aattatataa actaatttgt gtgatttcgg ttgcatagtt gcaaaagtgg
     3241 attaacccct tgtgacagta agaaaaaaat tgcaactgga aaatttaaaa cgagttgcat
     3301 agctgtaaat ttcagttgca cataatcgga agagacttgc agttatacaa aattgcctaa
     3361 gatactaaag ttactaaaaa acgtttaagt cactaaaaga tactaaaatt ctagaattca
     3421 gtaaccacag tttttacttt catattagtc tttttgacaa aaagttactg agttactata
     3481 aattatttct ttttagtaga aatgtataat agagaattaa ggtaataaaa gattatataa
     3541 aataccgtga attttcagta actcagtaac caacagcctg tatgtattga catcactgtg
     3601 tttacagtgg ttattgaaaa acataaattc agtggttttt agtatctaac gtggttgctt
     3661 tagtaactgg atagattgga ggactagagt aacatgaata gaaatagaat gaaacaaatc
     3721 atcttagaat atataaagaa caatgacagc acttcatttg tagagattga gaatgtattt
     3781 gaagaacaag gttttaaata caaaggtaac ggcgcatata ccagtggcaa ccataagaat
     3841 attatatttt ggatgggttg gaatgaagaa gcgtttaaca ttgtggcaga tttaaaacgc
     3901 gacgggctaa ttgaaatgca aatttgtccg ccaatgtact atcttattga tggtaaaggt
     3961 ctaaggctac caattgtaaa gaataaaaat ataaaaatag atcattggct accagtaaca
     4021 tttagtttag taaattaaaa catgcagata agcatttaaa atatcaagta tagatatggg
     4081 agtgtgtata tttattaatc ataaaagaat cgcatatcaa ataatcaaac atacacctgt
     4141 gaacgttcaa ttaatgaata cgaaatatat aaatcgcgtt gtaaggcgct atacaaaaaa
     4201 gcgtgctgat tttgaactta ttaaaatgta tatctatcag attaaaagga gagaggcaag
     4261 acgtgctaga tcatacaaat gaatctgtaa aacgtgtgtt taagtataga gataagcaag
     4321 aactattaaa taagtatcac gaattattag acaaatactt tcttcataac atttcgttct
     4381 atgaagaaga taaaaacaac gaacgtatat atggtttagt tgttcatact aaaaacaaat
     4441 taggagatga agattatgaa agagtttaaa gaacaatttg ggtatcaact atctaacttt
     4501 gatgatatgg atattaaagg atatgccaat ctatatcaaa aggatatagg taaagatgta
     4561 agtatgattg aacaggggtt aaagcaatta agtattactg aaacagaagt tttattacca
     4621 gagcaaatta acaaaaaatt attaggagtg ttaaatatga atgaagtaaa tcaatcaagt
     4681 aatacatggg taggtacctt aacaaaacaa ttagttagta gtgagaataa ctttaatatt
     4741 caagagttac caactgcacg taaagaagta tttaaagagt tattggtgaa ttgtgagcta
     4801 cctcaatcac tacgtgaggt gattaccatt actgatgatg aacatgttga atctatacca
     4861 tcactaacat acattaaaga taagttagct actaacggta ttgagctatc acttaatggc
     4921 agtagtaaat actttgatag acgtgaggga catatctaca cagaagtatc agacagtgta
     4981 gttcatggct ctgatagaac attagatgat ctacttaaag agatatttat taatgagtgt
     5041 gtcgcatatg aaacaacatt gttattagat aagaataatt caagtggttt aattgataaa
     5101 ggtaaccaag acttatcact ttataatcaa ggtatcaaag aagtaagtaa cacatcaata
     5161 tacgatggta tcaagcaagc gatgaaagat ataccacaaa cgtttaggcg taatgtatct
     5221 attgtgatga acacagaaca tcatgataag ctcatcaaag agttggcaca gatgggatta
     5281 ggtggacttg caggtgattt aagtaaccta tttaatgtga ctaatgtagt ggttactgat
     5341 gatgcacatg atgtctttgt tggagacttt ggacatgcca tatatgctaa atacgaacct
     5401 attatgtata acaaaaagaa acaagcatta aaaggtattt atcaatttgc attgaactat
     5461 gtgtttgata taaaaataat tccagagtta ttgcgcatag ttaatattaa ataaaaaaat
     5521 tatttaaata aaattttata gccccctgcc catcggctta aaatgttttt tcgccgggta
     5581 ccggcggggg cccttcgctt gcaacgcgga taaactttta tgaaaggggg ataacttgat
     5641 ttatcccttt ttcagatact tataaatatt aaattcgata tgtaaaggtg tggttatttg
     5701 gtatctgact ttgaaagcaa tgataaaatc actgaagtag aacttttaat gcattacaat
     5761 ccgaaagtca ttaatagcaa aattaaagcg atgagatcac aaatagaaag cttatatcac
     5821 ttgaacatga accatgtgat cacgaatgat aacgatatgc tggtatctgt atcatatcca
     5881 ttagataaat tagtacttta tattatagaa gagaaagata aacttgaata ttacatgaaa
     5941 acagctcagg ataggctaaa tttgtttaaa aatatcatca aaaactattc tgaaaatgaa
     6001 caacaggatg ttatgaggta catgttaagt agtgggaaag ttaaaaatga aggagtcatt
     6061 gaacgtttaa aagtagatat ttataaagtt gaaagtagaa agcgtcaaga acgacaaaac
     6121 cagcgagaag aattacatcg aatagagttt aataagcatt tagaacaagt taaaaaagaa
     6181 ctttcataga taagcatgat attaacggga atattcctat attcattaat attggtgaat
     6241 gggacggaga tgatgaggaa ttagataagg cagtgcaaga tgtatctaac gctaaccata
     6301 atcatactgt gattgtggat gatataccgt tagaggattg aagaaaatgg aagctatgct
     6361 atttataaat taatactaat tagtttgata ccatagctta tttactgaga aagtagactt
     6421 aaatgtaaca acaccagtgt ttattgataa tattggtggg tttgaggagt agcaataaaa
     6481 taaaggaggt aattgtgtaa aatatctctt tttgttattt cttattta
//
LOCUS       DAMQYT010000139         6484 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMQYT010000139 DAMQYT010000000
VERSION     DAMQYT010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.141"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            23..523
                     /gene="ftnA"
                     /locus_tag="PGA33_002036"
     CDS             23..523
                     /gene="ftnA"
                     /locus_tag="PGA33_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HDG6473838.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
     gene            complement(605..697)
                     /locus_tag="PGA33_002037"
     CDS             complement(605..697)
                     /locus_tag="PGA33_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473839.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            912..1466
                     /locus_tag="PGA33_002038"
     CDS             912..1466
                     /locus_tag="PGA33_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDG6473840.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKIHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            complement(1534..2604)
                     /gene="dinB"
                     /locus_tag="PGA33_002039"
     CDS             complement(1534..2604)
                     /gene="dinB"
                     /locus_tag="PGA33_002039"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDG6473841.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(2851..3381)
                     /locus_tag="PGA33_002040"
     CDS             complement(2851..3381)
                     /locus_tag="PGA33_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HDG6473842.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            complement(3551..4912)
                     /gene="rlmD"
                     /locus_tag="PGA33_002041"
     CDS             complement(3551..4912)
                     /gene="rlmD"
                     /locus_tag="PGA33_002041"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDG6473843.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPLAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            complement(4993..5940)
                     /locus_tag="PGA33_002042"
     CDS             complement(4993..5940)
                     /locus_tag="PGA33_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDG6473844.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
BASE COUNT         2097 a         1179 c          922 g         2286 t
ORIGIN      
        1 tacaaataag aggtgtatca aaatgttaag taaaaattta ttagaagcat taaatgatca
       61 aatgaaccat gagtactttg cagcacacgc atatatggca atggcagcat actgtgataa
      121 agaatcgtac gaaggatttg caaacttctt cattcaacaa gctaaagaag aacgtttcca
      181 tggacaaaag atttataact atattaacga cagaggtgca catgcagaat tcagagcagt
      241 ttcagcacca aaaattgact tttcaagcat actagaaact ttcaaagaca gcttatctca
      301 agaacaagaa gtaacaagac gtttctataa cttatctgaa atcgctcgtc aagataaaga
      361 ttatgcaact atctcattct taaactggtt cttagatgaa caagtcgaag aagaatcaat
      421 gtttgaaact cacatcaatt atttaactcg tatcggcgat gacagcaatg cattatatct
      481 ttacgaaaaa gaacttggcg ctcgtacatt cgacgaagaa taattaaaca tcactacaat
      541 agacagataa atatcatacg acatgatagg catttgggtc acttacaata acccaatgtc
      601 tatattattt tgctttacgg agatcactag attcattttc tgaatcattg atctgcgttt
      661 tttcattttc aaggctaatt attgtatttt tagtcattta ttttttaaac tactaatgtt
      721 aataactcta aatttgatgt tgaattaatt tgacgatttt aaagcatatc atcatttact
      781 ttttaatcag agttacatcc aaatgataga tttcacgtta taccttcacg tataatatta
      841 tgtatcgttt gtaagcaaat gactaaaagt ctattaatat atacatttaa ttaattgaaa
      901 ggattgacta catgatacaa gatgcgtttg ttgcacttga ttttgaaaca gcaaatggta
      961 aacgtacaag tatttgttct gtcggaatgg ttaaagtcat tgatagtcaa ataacagaaa
     1021 catttcatac tcttgtgaat ccgcaagact atttttcaca acaaaatatt aaaattcatg
     1081 gcatacaacc agaagatgtt gaaaatgcac ctacgtttga ctacgtattt ccatatatga
     1141 tgcaatttat tgcagattta cctgttgtcg cacataacgc ggcatttgat atgaacgtct
     1201 tacatcaaag cattcaaaat attggtttac caactccaaa tttaacttac ttttgtagtt
     1261 atcaacttgc taaaagaacc gttgattcgt atcgatacgg tttaaaacat atgatggagt
     1321 tttatcaatt agattttcat ggtcatcatg atgcattgaa tgatgccaaa gcatgcgcaa
     1381 tgattacttt taggctactg aaaaattatg aaaatttaac atatgtaact aatatttatg
     1441 gtaaaaatct aaaagataaa ggctaggact aaataaaata ctcccttcaa aagtaagcat
     1501 tgtaaaaatg taaactttgc agggagcttt attttatata aagtcatata tcgtcatatt
     1561 tttataagtt gattgttcta aattacctac agtgacacca ataagtcgaa ttggtacatc
     1621 agggtctttt aaatcgttat aaagtaaata tgcaatatta taaatatctt cttcagaact
     1681 aaccgaatct cttaaactca tctgtttaga tagcgtttca aattgataag ttttaatttt
     1741 aaccgttaca gttttagctg acttctgtaa tttatttaga cgttcagctg ttttacctga
     1801 caattcccat acttttctta aaatctcttc atcatcattc acgtctgttg caaatgtgcg
     1861 ttcagtccct actgattttc ttactcttga tgatttcact tcactatggt caataccgcg
     1921 tgccttgtta tataaacccc gacctctttt tccaaacaaa cgtattaatt caaattccgt
     1981 tttctcatat aaatctctac cgttaaaaat accattatca tgcattactt ttttggaagc
     2041 tttacctacg cctggaaaat ctccaatatc caatgtcatc aaaatatcat ggacattttg
     2101 ataatcaatc acagtcatac catcaggttt attcatacca ctcgctaatt tagctaaaaa
     2161 tttgttataa gaaacacctg cagatgctgt taaatgtgtc tgctctagaa tatcttttct
     2221 aatatactga gcaattttcg aagcaggaag gtctggtctc actaattctg taatatctaa
     2281 atacgcttca tccaatgaca tcggttctac cttatctgta taacttcgga aaatagacat
     2341 aatctgcgca gatgtttctc ggtaagcacc aaaattactt gtgacaaagt atccatttgg
     2401 acataattta tgcgcttgtg acataggcat tgctgaatgg acgccgtatt ttcgtgcttc
     2461 ataggatgcc gtagagacaa cacccctact gcttgcttta ccaccaacaa tgactggttt
     2521 ccctttcaat ttggggttat ctctcatttc gacttgtgca aaaaaatagt ccatatctat
     2581 atgaataatt cgtctctcag tcaagtgctc acctccctac taatttttac ttttataacg
     2641 cacaaaaata tctcaacata attatacgct gtgtacgatt tttttacata aatcttgcac
     2701 ttagcgataa ctatattgag ataactacaa gttgttataa aatcaattgc tatttaagca
     2761 tgatgatgaa gacgattgag taagaaaaca taggtaatct gaaataattc aagcaaattc
     2821 attttgttgg tatcatcata ttaaaattta ttattgagtc ggcttttgat gatacaaata
     2881 aatactatct tcatcttgtt gaacatattt aaaactacta gatattgtta atgcaacaag
     2941 cagaatatct ataaagcaat aagcggtatg catactaaac ataaaaataa gtgatgaata
     3001 accaaatacc ttaattaaaa taagcaagcc agtacttaat aggattagtg gtgacagcat
     3061 aataattgag aattgccatt tgttgaagca agcatctgct gtttggaata agattctgtc
     3121 tttttttata ttaaacatag gtttgctatc ttttttaaat aaaagaaata atgctctatg
     3181 gataagttca tgtaaaatca ataaaataat gaatccagca aacccatata caagattgat
     3241 gatgatattt tgatcgacaa ccgctgtgac acctaacgcc cacttatacg taaataaaat
     3301 cacgaataac gcaataacaa gttgcaagat aataaacctt cgcatttgaa aattatttgt
     3361 cgttaaatca attttatgca ttaccaaccc tcccgatcat gacattctta ttcttcttta
     3421 aatatagtat acaatgtcac atttaattta aaaagttcat atcaagaaag taaattggct
     3481 gtaataaaat tttaatatac gacttctttc ttcacttatt aaggcgaaat tttatctcaa
     3541 atcatgtgcg ctatttcaaa ttgaataatg ccactgtctc aacatgtgtt gtttgtggaa
     3601 acatatctac cggtgttacc tcttcaagtt gatatttttc agctaataat aatgcatcac
     3661 gttgctgtgt tgcgggatta catgaaatat agacaatacg cttaggttct aatgtaagca
     3721 aagtctgaat aaacgtttcg tcacagccct ttcttggcgg atcaaccatt acaacatctg
     3781 gtttaatccc ttgtgctttc cattgtaaaa taacttcttc agctttccca cagacaaaag
     3841 ttgtattatt gcattggttt atagtcgcat tttgttgtgc gtcttcaatt gcagaaggta
     3901 ctacttcaac accgtataca tgttttgcaa gtggtgccat atatagccct attgttccaa
     3961 taccacaata ggtatctaat acaacttcat tacctgtcaa ttgcgcatac tcaattgctt
     4021 tattatataa tttctctgtt tgttcagaat taatttggta gaatgactga tcacttattt
     4081 taaatgtact atctgttaat tgatcaataa ttgtatcttt accatatagc gttatagatt
     4141 gacgtcccat aataacatta gagtggctat cattaatgtt ttgtttaatg cttgtcacat
     4201 taggaaatgc atctaatatc ttctcaacaa cagcattttt ttgtggccac tttttaccat
     4261 tagttacaaa aataatcatc atttcgtctg tatgatatcc tgttcttaca accaaatgtc
     4321 tcattaaacc ttttttcaat tgttcttgat aaatacttac atttaaatct tttaaaatag
     4381 atttaacttc attcatcact tcttgatgtt gtgaatcttg tattaaacaa ctttccatgt
     4441 caataatgtc atggcttctt tgacgataaa agcccataat aacttcattc tgttcattct
     4501 taccaactgg aatctgggac ttgtttcgat atctccaagg atctgtcatg ccaactgtat
     4561 cgttaatctt agaattatca aaatgcgctt ttcgctgaaa caaattaatc acttgttcct
     4621 ttttcatttc aagttgtgct tcgtatgata agtgttgaag ttggcaccca ccacaacgtt
     4681 cataatatat acatggcggt gcaacacgat tctcacttct cgtatttata ttaagcaact
     4741 tacctatcgc aaagttcttt tttactttga taattttata ttcaatttgt tcattaatta
     4801 aagcttgtgg tatgaaaata ggaaagcgat ctatttttac gacaccatgg ccttcatgcg
     4861 ttaaatcaac aactgttccc gtttttatgt catttttagc tattgcttgc aaaattttac
     4921 ctccaaaatg aacaggttag gaacaaaatt atgcgcttcc taacctgcca ttatatattt
     4981 cactatttct gtttattctt cgattaaatt gtcatcaaca tgatcattat ttattaactc
     5041 ttcatttaca atatcattag gtgcaaagac atctatatga cgttctagat ttaagaaatt
     5101 cgctggtaat ttaccaccat attctccatc tacatttagt tgtaagtctg tgaatgatga
     5161 aatattaatt gcctttgctt tttcataaat aactttagga tgcttagtat gttctcctct
     5221 tgaagctaaa gtcataatat gaccaagttc tgcaaggttt gatttttcaa ctataattaa
     5281 cgtaaaatag ccgtcatcta acttagcgtc cggcactaat ttttcaaatc ctgccattga
     5341 atttgttaaa cctaaaaaga ataataatgc ttctccttgg aaaacattac catcatattc
     5401 aattcttaaa tctacagctt tcatttgagg taacatttcg aaacctttga tgtaataagc
     5461 aaatggacca acaatagatt tcaatttact cggtgtttca taagagactt gcgtcaattg
     5521 tccgcctgca gctaaattaa taaagtatcg attattcatt ttaccaatat ctactttagt
     5581 agaatgacct tcaatgatga catcaagtgc ccccatgatg tcattaggta tatgcaatgc
     5641 acgtccaaag tcattaacag tacccatagg aatgacacct agcttaggac gattaggctt
     5701 ttctgcgata ccattaacta cttcatttaa tgttccatca ccacctgcag cgattaatac
     5761 atcataattt tcatgcatag ctctttctgc ttcaagtgtg gcatcaccta ttttctcggt
     5821 tgcatatgca ctcgtttcat atcccgcttt ttctaatttt attaaggcat caggtaattc
     5881 tcttttaaat agctctttac ctgatgtcgg gttataaatg attctagcac gtttcctcat
     5941 atcttatccc tctacttaaa attcatatat tttaacttca tctttgtttc gtctaatagg
     6001 gagtgggaca gaaataatat ttaacaaaat ttatttcgtt ctaccccaac ttgcattgtc
     6061 tgtagaattt cctttcgaaa ttctctatgt tggggcccca ccccaacttg cacattattg
     6121 taagctgaca gaaagtcagc ttctttgttt gggggccccg ccaacttgca cattattgta
     6181 agctgacaga aagtcagctt ctatgttggg gccccactag aattgaaaaa agcttgttac
     6241 aagcgtattt tctttcagtc aactacagcc aatataacat tgtagtgcct aggacattga
     6301 atttatgacc caggctcagt cttatttcat cattcttaat atcgttaaag accaacttgt
     6361 atcttaaaca aatactatct caatatgtac aaagcttgtt atttattcag cattttttgc
     6421 cgttcttcat tatatagact tcgtcagtta tgctatttta cctttaaaat gatgttgtaa
     6481 atat
//
LOCUS       DAMQYT010000140         6474 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMQYT010000140 DAMQYT010000000
VERSION     DAMQYT010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.142"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..2520
                     /gene="mutS"
                     /locus_tag="PGA33_002043"
     CDS             <1..2520
                     /gene="mutS"
                     /locus_tag="PGA33_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDG6473845.1"
                     /translation="FYEDAKEASRVLEITLTKRDAKKENPIPMCGVPYHSADSYIDTL
                     VNNGYKVAICEQMEDPKQTKGMVRREVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQP
                     EIALSYCDVSTGELKVTHFNDEATLLNEITTINPNEVVINDNISDNLKRQINMVTETI
                     TVRETLSSEIYSVNQTEHKLMYQATQLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMD
                     FYAKRNLELTESIRLKSKKGTLLWLMDETKTPMGARRLKQWIDRPLISKEQIEARLDI
                     VDEFSAHFIERDTLRTYLNQVYDIERLVGRVSYGNVNARDLIQLKHSISEIPNIKALL
                     NSMNQNTLVQVNQLEPLDDLLDILEQSLVEEPPISVKDGGLFKVGFNTQLDEYLEASK
                     NGKTWLAELQAKERQRTGIKSLKISFNKVFGYFIEITRANLQNFEPSEFGYMRKQTLS
                     NAERFITDELKEKEDIILGAEDKAIELEYQLFVQLREEVKKYTERLQQQAKIISELDC
                     LQSFAEIAQKYNYTRPSFSENKTLELVESRHPVVERVMDYNDYVPNNCRLDNETFIYL
                     ITGPNMSGKSTYMRQVAIISIMAQMGAYVPCKEAVLPIFDQIFTRIGAADDLVSGKST
                     FMVEMLEAQKALTYATEDSLIIFDEIGRGTSTYDGLALAQAMIEYVAETSHAKTLFST
                     HYHELTTLDQALPSLKNVHVAANEYKGELIFLHKVKDGAVDDSYGIQVAKLADLPEKV
                     ISRAQVILSEFEASAGKKSSISNLKMVENEPEINQENLNLSVEETTDTLSQKDFEQAS
                     FDLFENDQESEIELQIKNLNLSNMTPIEALVKLSELQNQLK"
     gene            2533..4542
                     /gene="mutL"
                     /locus_tag="PGA33_002044"
     CDS             2533..4542
                     /gene="mutL"
                     /locus_tag="PGA33_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDG6473846.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMEENQNDEIVIK
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDGTTVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            4548..5090
                     /locus_tag="PGA33_002045"
     CDS             4548..5090
                     /locus_tag="PGA33_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDG6473847.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
     gene            5555..6373
                     /locus_tag="PGA33_002046"
     CDS             5555..6373
                     /locus_tag="PGA33_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDG6473848.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
BASE COUNT         2469 a          860 c         1210 g         1935 t
ORIGIN      
        1 ttttatgaag atgccaagga ggcatcacgt gtacttgaaa ttactttaac taaaagagat
       61 gctaaaaaag aaaatccaat tccgatgtgt ggtgttccgt atcattctgc agatagttat
      121 atagatacac ttgttaataa tggatataaa gtagctattt gtgaacagat ggaagatccg
      181 aaacaaacga aaggtatggt tagacgtgag gtagtaagaa ttgtgactcc aggaactgtg
      241 atggagcaag gtggtgtaga tgataaacaa aataactata ttttaagttt tgttatgaat
      301 caacctgaaa ttgcgcttag ttactgtgat gtttctactg gcgaattaaa ggttacacat
      361 tttaatgatg aagcgacttt attaaatgaa attacgacga taaaccctaa cgaagttgtt
      421 atcaatgaca atatttccga taatttaaaa agacaaatta atatggtgac agaaacaata
      481 acagtcaggg aaacgttatc atcagaaatc tatagtgtga atcaaactga acataaatta
      541 atgtatcaag cgacacaatt attgctagat tatattcatc atacacaaaa acgtgattta
      601 tcgcatatcg aggatgttgt tcaatatgca gctatagatt atatgaaaat ggatttttat
      661 gctaagagaa accttgagtt aacggaaagc attcgattaa aatcaaaaaa aggaacgcta
      721 ctttggctaa tggacgaaac gaaaacacca atgggagcac gccgcttaaa acaatggata
      781 gatagaccac taataagtaa agaacaaatt gaagcacgat tagatatcgt tgatgaattt
      841 agtgctcatt tcatagaaag agacacctta agaacatatc ttaatcaagt gtatgatatt
      901 gaacgtcttg ttgggcgtgt tagttacgga aatgttaatg cgagagattt aattcaactt
      961 aaacattcca tttctgaaat accgaatatt aaagcattac taaattctat gaatcagaat
     1021 actcttgtac aagttaatca actagaaccc cttgatgatt tacttgatat attagaacag
     1081 agtttagtag aagaaccacc aatttcagtt aaagatggcg gactattcaa agttggtttt
     1141 aatacgcaat tagatgaata tcttgaagct tcaaaaaacg gaaaaacatg gttagcagaa
     1201 ttacaagcca aagaaagaca acgtacagga ataaaatcat tgaaaataag ctttaataaa
     1261 gtgtttggtt attttataga aataacacgt gccaacttgc aaaattttga accaagtgaa
     1321 tttggttata tgaggaagca aacgttatcg aatgctgaac gttttataac tgatgaactt
     1381 aaagaaaaag aagatatcat tttaggtgcg gaagacaaag ccatcgaatt agaatatcaa
     1441 ttatttgttc agctacgtga agaagttaaa aaatatactg aacgtttaca acaacaagct
     1501 aaaattattt cagagctaga ttgtttacag agctttgcag aaattgctca aaaatataat
     1561 tacactaggc cttcatttag tgaaaataaa acattagaat tagtggaatc taggcaccca
     1621 gtagtggaaa gagtaatgga ttataatgac tatgtgccta ataattgtcg attagataat
     1681 gaaacattta tatatttaat tacaggtccg aatatgtctg gtaaatcgac atatatgaga
     1741 caagttgcca taattagtat aatggcccaa atgggagctt atgtcccttg taaagaggca
     1801 gtgttaccta tatttgatca aatattcact agaataggtg cggcagatga tttggtttca
     1861 ggtaagagta cgtttatggt agaaatgcta gaagcacaaa aggcattaac ttatgcaaca
     1921 gaggatagtt tgattatttt cgatgaaatt ggacgtggta cttcaacgta tgacggttta
     1981 gctttagcgc aggcaatgat agagtatgta gctgaaacat cacatgctaa aacgttattt
     2041 tcaacacatt atcatgaatt gacaacatta gatcaagcat taccaagtct aaaaaatgtt
     2101 cacgtcgctg ctaatgaata taaaggtgaa cttatattct tgcataaagt caaagatggt
     2161 gcagttgacg atagttatgg tattcaagtt gcgaaattag ctgatttacc tgaaaaagtt
     2221 attagcagag cacaagtgat tctaagcgag tttgaagcgt ctgctggtaa aaaatcatcg
     2281 atatcaaatt taaaaatggt cgaaaatgaa cctgaaatta atcaagaaaa tttaaactta
     2341 agtgttgaag aaacaactga tactttatct caaaaagact ttgaacaagc atcatttgat
     2401 ttgtttgaaa atgatcaaga aagcgagatt gaactacaaa ttaaaaattt gaatttatct
     2461 aatatgacac caattgaggc attggtgaag ttaagtgaat tacaaaatca attaaaatag
     2521 aggtgttgca aaatggggaa aattaaagaa ctccaaacct cattagcaaa taaaatcgca
     2581 gcaggtgaag tagttgaaag accgagttct gttgtgaaag aactgttgga aaatgctata
     2641 gatgcaggcg ctacagaaat aagcattgaa gtagaggaat ctggcgtcca atctattcgc
     2701 gtagtcgata atggaagcgg aattgaagcg gaagacttag gattagtatt tcatagacat
     2761 gcgactagta aattagatca agatgaagat ttatttcata ttaggacatt aggattccgt
     2821 ggtgaagcac tagccagtat ttcatcagtt gctaaagtaa cattgaagac ttgcacggat
     2881 aatgctaatg gaaatgaaat atatgtagaa aatggtgaaa tattaaatca taagcctgca
     2941 aaagcgaaaa aaggaacaga tatacttgta gaatcattat tttataatac accagcacgt
     3001 ttaaaatata ttaaaagttt atacactgaa ctaggtaaaa taacagatat tgtcaacaga
     3061 atggcaatga gccatccgga cattcgaata gcactcattt cagatggcaa aacaatgtta
     3121 agtacaaatg gttcaggacg aactaatgaa gtgatggcag agatttatgg gatgaaagtt
     3181 gcacgagatt tagtacatat atctggagat acaagtgatt atcacattga aggttttgtt
     3241 gcaaagcctg aacattctag aagtaataag cactatattt ctatttttat taatggacga
     3301 tacattaaaa actttatgct aaataaagcg attttagaag gctatcatac actcttaaca
     3361 ataggtaggt tcccgatttg ttatattaat attgaaatgg atccaatctt agtagacgta
     3421 aatgttcatc caacaaaact agaagtgcgt ttatcaaaag aagagcaact ataccaattg
     3481 attgtgagca aaatacaaga agcatttaaa gaccgtatat taattcctaa aaataacttg
     3541 gattatgtgc caaaaaaaaa taaagtatta cattcattcg aacaacaaaa aatcgaattt
     3601 gaacaaagac aaaacacaga gaataaccaa gagaagacgt tttcatctga agaaagtaac
     3661 agtaagccat ttatggaaga aaatcaaaac gatgagatag ttataaaaga agattcatat
     3721 aatccattcg taacgaaaac atctgaaagt ttaatagctg atgatgaatc ttctggttat
     3781 aataatacac gtgaaaaaga tgaagactac ttcaaaaaac aacaagaaat tttacaagaa
     3841 atggatcaaa catttgattc aaatgatggt acaactgtgc aaaattatga gaataaagcg
     3901 tctgatgatt attatgatgt aaacgatatt aaaggaacaa aaagtaaaga ccctaaacga
     3961 agaattccat atatggaaat tgttggacaa gtacatggaa cgtatattat tgctcaaaat
     4021 gaatttggca tgtacatgat tgaccagcat gcagctcaag aaagaataaa atatgaatat
     4081 tttcgagata aaataggtga ggttaccaat gaagtacaag atttattaat cccgttaaca
     4141 tttcattttt caaaagatga acaattagtc attgatcaat ataaaaatga gcttcaacaa
     4201 gtaggtatca tgttagaaca ttttggtggt catgattata ttgtaagtag ctatccagtt
     4261 tggttcccta aagatgaagt agaagaaatt attaaagata tgattgagct aattttggaa
     4321 gagaaaaaag tagatatcaa aaaattacgt gaagatgtag caatcatgat gtcatgtaaa
     4381 aaatccatta aagcgaatca ttatttacaa aaacatgaaa tgtctgattt aattgatcaa
     4441 ttaagagaag cggaagatcc atttacatgt ccacatggtc gtccaataat cattaatttt
     4501 tcaaaatacg aattagaaaa attatttaag cgtgtgatgt agagaggatg aatcaagtga
     4561 ataacaacat attgcctgct ataagaaaca ttaaagattt agagaaactg attaaaacag
     4621 actataaaat gtgtgtgctt ctagatatgc atataggaca tataaaaagt attatggaat
     4681 tgctgaagca aaatcatata gagtgtttta ttcatataga tttgataaaa ggtttaagcc
     4741 acgatgaatt tgcaagtgaa tttattattc agcaatacaa gccaaaaggt atcgtatcga
     4801 ctaaatctaa agtaataaaa aaagctaaat cattaaatac tttaacgatt tttagagtat
     4861 ttattattga tagtcaagca ttgaaacgca gtatagattt gataaaaaaa gttgaacctg
     4921 attttgttga agtacttcca ggtgttgcga gtaaagcgat tcatcatatt cagaaagaaa
     4981 caaacacaca agtcattgca ggtggcctaa ttaatacaat agatgaagtc aatgaagctg
     5041 ttaaaaatgg agcgaaatat gtaactacta gttatgataa actttggtaa atcataagtc
     5101 tgcaaaaatt tcatttttca cgagatttag tcatataaca taataaaaaa gccgttttca
     5161 tatgggatat gcagtaggta tcaaacaaaa aataatcaaa taaaataatt agcacctcta
     5221 aaacgattaa aatcattaaa gttttagagg tgtttattag taaaaaatgc aactagtcat
     5281 aaaaaatatg gctgaagaat gtcaattaaa caaaagagcg tcataaataa attagttagg
     5341 attggtattt ctagttttca aattaaacta cttttataaa gacttgaaaa attaaatttg
     5401 acaaaatttt tacaaaaacc attgacaacg ctttcatatc gatagtatca taacaaatat
     5461 aataaaagtt aatacataga atagagacgg gagatttcta cgagccaaac tgctagtgta
     5521 ggaatctctt tgtctttttg ggaggacatt taatatgaat gtatatttag cagaattcct
     5581 aggaactgca atcttaatcc tttttggtgg tggcgtttgt gccaatgtca atttaaagag
     5641 aagtgctgcg aatggtgctg attggattgt catcacagct ggatggggat tagcggttac
     5701 aatgggtgtg tttgctgtcg gtcaattctc aggtgcacat ttaaacccag cggtgtcttt
     5761 agctcttgca ttagacggaa gttttgattg gtcattagtt cctggttata ttgttgctca
     5821 aatgttaggt gcaattgtcg gagcaacaat tgtatggtta atgtacttgc cacattggaa
     5881 agcgacagaa gaagctggcg cgaaattagg tgttttctct acagcaccgg ctattaagaa
     5941 ttactttgcc aactttttaa gtgagattat cggaacaatg gcattaactt taggtatttt
     6001 atttatcggt gtaaacaaaa ttgccgatgg tttaaatcct ttaattgtcg gagcattaat
     6061 tgttgcaatc ggattaagtt taggcggtgc tactggttat gcaatcaacc cagcacgtga
     6121 tttaggtccg agaattgcac atgcgatttt accaatagct ggtaaaggtg gttcaaattg
     6181 gtcatatgca atcgttccta tcttaggacc aattgccggt ggtttattag gtgcagtggt
     6241 atacgctgta ttttataaac atacatttaa tattggttgt gcaattgcaa ttgttgtagt
     6301 tattattact ttgattttag gttacatttt aaataaatca tcaaaaaaag gtgatatcga
     6361 atcaatttac taaaataaaa agaaacgtaa atagcataat ttaacatgtt tgattcatgg
     6421 attatgctat tttttcgcca aaatttaaca gattttgtac aatgggttag cgat
//
LOCUS       DAMQYT010000141         6466 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMQYT010000141 DAMQYT010000000
VERSION     DAMQYT010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.143"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            25..831
                     /locus_tag="PGA33_002047"
     CDS             25..831
                     /locus_tag="PGA33_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDG6473849.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLDELQKGKHYGATWQDKERISWGISDQNLSKIA
                     AEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVIDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(894..1550)
                     /locus_tag="PGA33_002048"
     CDS             complement(894..1550)
                     /locus_tag="PGA33_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDG6473850.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            1663..2136
                     /locus_tag="PGA33_002049"
     CDS             1663..2136
                     /locus_tag="PGA33_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDG6473851.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(2259..3470)
                     /locus_tag="PGA33_002050"
     CDS             complement(2259..3470)
                     /locus_tag="PGA33_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDG6473852.1"
                     /translation="MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(3652..4311)
                     /locus_tag="PGA33_002051"
     CDS             complement(3652..4311)
                     /locus_tag="PGA33_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473853.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            complement(4371..5513)
                     /locus_tag="PGA33_002052"
     CDS             complement(4371..5513)
                     /locus_tag="PGA33_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDG6473854.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            5781..6167
                     /gene="gtxA"
                     /locus_tag="PGA33_002053"
     CDS             5781..6167
                     /gene="gtxA"
                     /locus_tag="PGA33_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HDG6473855.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFLVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLIITIPITFILSKWILKDSN"
     gene            6301..6408
                     /locus_tag="PGA33_002054"
     CDS             6301..6408
                     /locus_tag="PGA33_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDG6473856.1"
                     /translation="MFNLLINIMTSAISGCLVAFFAHWLRTRNNKKGDK"
BASE COUNT         2110 a         1019 c         1213 g         2124 t
ORIGIN      
        1 aatttgagta aagaaggtaa gggtatgaag atagggacaa tcgccgattt acatattgat
       61 cgtcataata aaaaaacatc cgaagattac ttagaggcat tggttgaaat agttaagtat
      121 aagaaattgg atatattgtt gattgcgggg gatatctcaa atcattatca attgacgcac
      181 caatttataa cgcaattgac aaaacaatta gatatacctg tgaaatttgt acctggaaac
      241 catgatttat gggaagttga aagtatgact acgcaagaca tttggaataa ttataagagt
      301 atgtcacagt gcttggtagg aaaaccattt atagtaaatg aagaatgggc aatcatagga
      361 catactggct ggtatgatta tagctttgca gcacaacgat tttcattaga tgagttacaa
      421 aaaggaaaac attatggtgc gacttggcaa gataaagaac gaatatcttg gggcatatca
      481 gatcaaaatt tatctaaaat agcggctgaa caagtgaaga aagatatatt agaagtagga
      541 aatagacgag tgattttagt cacacatgtt gtgacgcacc ctgatttcat tgttcctatg
      601 ccgcatcgta tattcgattt ttataatgca tttattggga caagtgattt caatcctttg
      661 tatgcgatgt tcgatatacc atatagtatt atgggccatg ttcattttcg taaaagtgtg
      721 atagatgatg gcagatgtta tctctgtccg tgtctaggct atccaagaca atggcgttca
      781 gaagatattt accaggaaat aaatgagacg atacaaataa tagaaattta aaatgcgcaa
      841 acctgaccca gtttgcgcat tttatgtttt acacacgcga gtaatgtgtt tacttacgtg
      901 tgtttatttt gttgctgatt ttcaattgta tatgaatgtg gttgcacata aatgcacttt
      961 cttcctggtg aattaaagct gtattccatt ttctctttac gaattttaat aatttgtttg
     1021 cgatttggaa tgatggcagg taaaactagg ccacgacgaa tatgactcca aagtggatcg
     1081 atataatagt aatagtaatc atcgtaccca atgagtaacg ttacgtgaat atttgaaact
     1141 tgtttcgctg gcttgttatc aaagcggaaa acacgtagta atggtttaga accaagatta
     1201 gtatggtata ttaacacagg ttgaccttga tcgataatac ctttaagatc ttctaacgat
     1261 ttaccagtgc cgtctacgat attaggattg tatttttgta aaaatggtac atatgcttct
     1321 ggaaatatcg tttgatgata attgccaagc ttaatgaata agtgatgtcc aacataacct
     1381 ttatgtggat tgttcggatg tgtcggccaa tgtctcataa tttctgtagc agggatatgt
     1441 tggttgttgt attgcaacat catggctgcg gaaacacctt cacaccccat gaccataggg
     1501 ataggaaata gctgactgat aggtttaact ggtaatattt ttcggttcat aatatagtcc
     1561 tcgcattgat tcaataaata tttaatataa ttatatagcg tcaatgcaaa atgtcctaaa
     1621 catatgtttt acatgagtga ataaaattaa tggagtgata aaatggaata tcaattacaa
     1681 caacttgcgt cgttaacgtt agtaggtatt aaagaaacgt atgaaaatgg acgacaggct
     1741 cagcaacata tagcagggtt ttggcaaaga tgttatcaag agggagtaat tgcggattta
     1801 cagttaaaaa ataatggtga tttagccggg atacttggct tatgtatacc tgaattagac
     1861 ggtaagatgt catatatgat tgcagttacc ggagataata gtgctgatat tgaaaaatat
     1921 gatgtcataa cattagcaag ttcaaagtat atggtatttg aagcacaggg cgcagtacct
     1981 aaagcagttc aacaaaaaat ggaagaggtt catcactaca tacatcaata tcaagcagat
     2041 acggtaaaat cagcaccatt ttttgagttg tatcaggatg gtgatactac aagtgaaaaa
     2101 tatattacag aaatttggat gcctgttaaa gggtgattga atagaaatgt ccttgctatg
     2161 aaggcgtaat tttagttgaa gattcgatct cataattcat aaagaagaac cctgacaaag
     2221 atagatatct ttgctagggt tctaattatg ttgaagtgtt atgcgacatg ttgcttttta
     2281 atcattttaa agtaaatgat ttgtagactg actaatagaa tggctgtaat gaagataata
     2341 atcatatatg gtgatgtatt aaattcgcct tttaagccaa ctaatggtgc aacagcgcca
     2401 cctaagataa attggaataa gccaagtaaa cttgatgcgt taccactgcc acctgttcgt
     2461 tcttccatag ccattgtgaa accaagcggt ccaattgacg tcacaggaca cacatttaag
     2521 aagaatgcga tgagtaagac ccaaagtggt aaatggaatg taagtgtcag gataattaaa
     2581 gcaacaccta ccacttgtat aatagttaaa atgattaata atatgtggcg atgtaatttt
     2641 tctactaata aagcaacgac ttgactgaca atgattaaac ctacaccgtt aacagcaaac
     2701 atgatactaa attgttgggg tgtcatatta tacaattttt gagtaataaa tggcgatgca
     2761 gatgaataac taaatagcat tacataagtt aaaccttgta atagcattgg aataataaat
     2821 gctggttttt tcaaaagact tccaaaatct ttaatgacgc tactaaaatt cacctgcttt
     2881 aattcatctt tagatgtttt aggtaattga gaagcgacgc caattaaaat gatgagtgcc
     2941 acaatagtta aaattgtgaa aatagaacgc catgttgcta cggataaagc taatccaccg
     3001 gctaatggtg caagaatagt gatgatgcca ttaactacca ttaaagatgc taaaaattta
     3061 gcgagtgcgt tgccactaaa tttatcacca gcagaggctt ttgcaatcac gatgacgcca
     3121 ccaccagtta aaccttgaat aaatcgtaaa gttaagaata atggcaattg atcaacaaac
     3181 atagacaaac ctgataccaa aatgaaaatg atcaaaatgg atacagcaat ccgtttgcga
     3241 ccaaaagcat cggataaagg tccaaagata aattggccta gtgcaagacc aatcattgtg
     3301 aatgataatg ttaattgaat ttctgatgta gtagaaccaa aatcaccttg cactttaggt
     3361 aatgatggta cgtacatatc tatagttagt gggccaaaag cggtcatgac gcctaacgta
     3421 attagcaata aaaatgaaaa atgcttcgtt ttggttttcg ttgattgcat gatcagactc
     3481 ctttaaatga tataattata taaattatat acgttttgtg tgtatttgta tgtacttaga
     3541 atacaagttt atataaaaaa tccctatact cttaatgcat catcacgaat aacgtagtga
     3601 tacagataaa gtatagggaa ttaatgttta tcatcaaagc aggtgtaata attaattagc
     3661 gccaccattg tcatctttaa ataagctttc taatttagat ttaatactgt cgaattttgt
     3721 attaatatca tttaacatgc tttcaatttt accttggctt gcattgtttt taagtgcatt
     3781 atttgcctct gtcaatttgt tgatttggtc ttgataaaat tgagttagct tagaatcagc
     3841 tttgttttgt tcttgctgct tcaagttgtc gatttgattt tgtaaatttt taataatgtc
     3901 atcagttttt tggttgtttt taatattttc tttagcatta tctacttgct ggttaatttg
     3961 gccgtcttga ttttcaactt gttttttata attttgagag actttagggt cattactaac
     4021 tttatcgcta tcattgttaa gtaaggcgcg tgtgacaaaa atagcgacaa taattaagag
     4081 aatggcaata atgatatata accatttttt tgaacggcgg gatttctcgt tttcttcttc
     4141 aagaatttgt tctcttcgat aatctctacc attatagtat cgttccgggt gttcttcagg
     4201 atcgccttta gcatattgtt gtctatacga tgcgtcttca gattgacgac gatgattttg
     4261 gttttgattg tcgtattgat atgaatcacg gtatttatca gttcttttca tgggtttcac
     4321 ctcattcaag ttttaactgc aaataataaa gctgatgatt tactttgaca ttattgtaat
     4381 ttcagaatac gagcaatgtc agttgcagtg tttaataaat tttgttcgct attttgtagg
     4441 acatcttcta aagtgctagg tgaagagatt atagaatagg cactatcaat accgaaatcg
     4501 taaacatgtt gataattttc gcctagactg ccacaaatcg cgatgacagg aatatgatat
     4561 tgttttgcag ctaacgcaac gcctacgggt gttttaccaa agatggtctg ataatccatg
     4621 cgtccttctc cagtaataac gaggtctgca tctttaattc tttgatgaaa atctgtaatg
     4681 tcaaagacga catcaatacc ttttgttaaa gttgtctcac aaaacgctaa taatgctgcg
     4741 cccataccgc cagctgcacc agaacctggt atttgattaa cggactttcc tgtgcacatt
     4801 tttatcttat catgataatg cgacattgcg aaatccaact ttggtatcat ctttgcatca
     4861 gcgccttttt gaggaccata aatataggta gcaccatttt cacccaataa aggatttgaa
     4921 acatcacagg ccactttaaa ggtcacctct tttaatcgcg aatctagatt ggttatatcg
     4981 atttgtgcaa tgtgagcaag attagcacca ttcatttgta ataagtcccc gtttacatca
     5041 gtaaacttta cgcctagtgc acttagcata cctgtaccac catcatttgt tgcactgcca
     5101 ccaatcccta aaataatggt cttagcacca tgatttaatg catctttaat tagttcaccg
     5161 gtaccatatg atgatgtgta taaaggatta cgttcctctt tttctaataa atccaaacct
     5221 gacgctgccg ccatttcaat aattgcaatt tgttgttcgt ctgcgcgtgc ataacatgct
     5281 tcaataggtc gcattaaagg gtcattaacg atgactgtat acttagtggc acctgttgca
     5341 tgcattaaag catctgtggt accttcacca ccatcagcca tcggaatgat atcataatga
     5401 agggtattcc cataaacatt agtaaaagcc tgttttataa tattgccaac ttgctgtgcg
     5461 gtcatacttt ccttaaatga gtcaggagcg atgacaattt tttgtaattg catagtagac
     5521 atcctctcat tactgttgtc gctcttattg tatataaaat tactagtgaa ctaaagtaat
     5581 ctctatagtg ctaatgagtc acgcgataaa tcagttacat tataatggcg tataaggatg
     5641 aggggaaaga gactaaattt gctgtgaaag ggtataaaga ttgaaataag catatttgaa
     5701 gtgaagcgga ttggctcgca tattcgagta tgttcgagaa cgtagttatg catatatttt
     5761 gatgttgagg agttacacat atgaaactaa cacaaacgca tgctgaaatt ttaaaattta
     5821 tcattgttgg cggcattaat acgttaaatt attatgttgt ctatttattg ctgttaaaat
     5881 tattacacat tgaatatatg attagtcata ttaccggatt tctagtcgct tttgtgattt
     5941 catattattt gaattgttat tttgtttaca gggtaaaacc tacttggaga aaattcatta
     6001 gttttccaat tacgcagatt gtcaacgtaa gcttacaaac agttttatta tatgtatttg
     6061 tatcctggct aaatttgcca gcagaaattg caccatttgc tggtttaatt attacgatac
     6121 ccatcacatt tatattatct aaatggattt taaaagatag caattaaagt agcgtaaacc
     6181 actaaaaaaa actttactta agccttaata ttgcgtgaaa tataataaat aaatgatata
     6241 atatgatggt cgcctaacat ctcgggcgtt actttaacgc agagaggagg tgtatgacgg
     6301 atgttcaatt tattaattaa catcatgact tcagctataa gcggctgtct tgttgcgttt
     6361 tttgcacatt ggttacgaac gcgcaacaat aaaaaaggtg acaaataacc aacacctaaa
     6421 taaatcccct cactatggca gtagtgaggg gatttggtgt atttag
//
LOCUS       DAMQYT010000142         6450 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMQYT010000142 DAMQYT010000000
VERSION     DAMQYT010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.144"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            1..573
                     /locus_tag="PGA33_002055"
     CDS             1..573
                     /locus_tag="PGA33_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HDG6473857.1"
                     /translation="MVSLFETKIFQFMTLNLFLAYIPFELSLLLRLFKPNKKYEWPLF
                     IIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLSEWKYFTYLLLGVFLAVYVMI
                     LIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVYFFNEPLHVLNHILES
                     LTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            570..854
                     /locus_tag="PGA33_002056"
     CDS             570..854
                     /locus_tag="PGA33_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473858.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(1009..2382)
                     /locus_tag="PGA33_002057"
     CDS             complement(1009..2382)
                     /locus_tag="PGA33_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDG6473859.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVTAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYNVFTSFYKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(2468..4022)
                     /locus_tag="PGA33_002058"
                     /pseudo
     CDS             complement(2468..4022)
                     /locus_tag="PGA33_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
     gene            complement(4305..4592)
                     /locus_tag="PGA33_002059"
     CDS             complement(4305..4592)
                     /locus_tag="PGA33_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473860.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFVKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
     gene            complement(4627..5535)
                     /locus_tag="PGA33_002060"
     CDS             complement(4627..5535)
                     /locus_tag="PGA33_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDG6473861.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIVQDIP"
     gene            complement(5638..>6450)
                     /locus_tag="PGA33_002061"
     CDS             complement(5638..>6450)
                     /locus_tag="PGA33_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDG6473862.1"
                     /translation="KIKIIMNEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYE
                     LKAIALKGDVNHVIIEATGIAHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQ
                     YTENTVSLMEDQLKLSDMIIINKIDLITDDSLEKIDKQLGMICASIPTYKTTYGKVSL
                     EELDLTVKDREISSHHHHHHGIKSMTYTFTGPIDRHLFYQFIMKLPESVLRLKGYVSF
                     RDQPNAIYEFQYAYGLPDYGIIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
BASE COUNT         2173 a         1155 c          925 g         2197 t
ORIGIN      
        1 atcgtctccc tttttgagac taaaattttt caatttatga ctttaaattt gtttttagca
       61 tatatacctt ttgagcttag tctattatta cgattattca aaccgaataa aaagtatgaa
      121 tggccactct ttatcatata tagttttata tttgtgttac ttttaccaaa tacattttat
      181 atggtaactg atttaatcca tttgaaccaa tttcaattca acttttacgc aggactaaat
      241 ttaagtgagt ggaaatactt tacctattta ctgcttggtg tcttcttagc ggtttatgtg
      301 atgattttaa tctattttga aatactaaca ttgacttctc accgttggct taatagatta
      361 attgttgtcg tgctaatgtt tttaaatgga ttcggaattt atattggtcg tttcttacgc
      421 ttacattccg tttatttctt taatgagccc ttacatgttc taaatcacat tctagaaagt
      481 ctaacgctta aaactgctat gtttgtatgt tttatggttg tcatgcaagc agcaattcta
      541 ttatttggaa aaggagtgcg tttgaacaag tgacaataaa tataatcgtt ttaatagtat
      601 ctatcattgt ctttcaattg attattggac acatatggca tgatattggt ttaagttatt
      661 taagaagtat cttactcatg atgctgccat ttggactagg tgtatttata caacaagtgt
      721 cgtattacga acgacaatat cctaaatggc aagtgcctca aaacataaaa gttagattga
      781 aatatattta tctcgctacg tttttagagt atgtcgtact atacctcact ttatttacgg
      841 acatactaag ataacatcaa attaataaga aaactaaata caacttatat atttgaggaa
      901 agccaatttc tatcacatca ataatagaaa ttggcttatt tatattgctt aagaacttta
      961 tattctgctc cagattttac tcatgccaga tctatcaatt gagccgaatt agtctagcgc
     1021 tttaaatgta gctaaaactt gtgtacgttt ttcttgatga tctaccattt gtcttggata
     1081 atgactacct aattcaatcc cctgttcaaa aaggttggac tcattgcgtt gtgtatcatg
     1141 caaatatttt gcatcaattt gattaaaaat cggaagatat gttttgatat acaaagcttt
     1201 agcatcaaag cgttcactct gtcttattgg attaaacatt ctaaaatacg gcactgcatc
     1261 cgtacctgta gaagcagacc attgccatcc atgaatattt gatgctgcat catagtcaat
     1321 aaggtgcttt ctaaagaatt tttctcccca tgtccaatct ataaataaat ctttggttaa
     1381 aaattgcgac acaaccattc tcattcgatt atgcataaaa ccagtttgtg tcaattccat
     1441 tattgctgca tcaatgattg gaaatcctgt ttgcccttcg caccatgcat taaaatccgc
     1501 ttcattttgc gaccatttta tctgtcgata tttaggtttg aaagcttggt atgaggtttc
     1561 aggatactgt gtcattaaca cataataaaa ttctctaaaa atgagttcac gtataaatgc
     1621 ttcatagttt gcctcatctt catcataacg cgccaataaa tcattaaaaa tttcaataat
     1681 atctaataat ccatatgcta aagcaacact tagctgactt gttaatactt caggtaaata
     1741 gtctcttccg ctttcgtaat tctgtatatc ttgatctaaa aaatgttgcc atttattctg
     1801 ttcaattatt tgtgaggatc caaatgctat gtcatcaaac actaaatcat caggtgatgc
     1861 aatgacaaag tcttcgaatg attttaaatc atagtgatat acgtctctat gcctcaagta
     1921 aggtcgccat tttttataga aactagtaaa aacattgtat ggctcccctt gttgattcat
     1981 tgttttactc gctttaaagt aatgattccc acgtatcaaa gtaacggcaa tgcgtgcttc
     2041 attgaataaa gaacgttgat gtaaaatatc atattcttca agatgataac tcataatatc
     2101 acccgcagtc actacttcag agatattgtt ttctttacaa aatgaaatga tatcgtcata
     2161 agacataaca taaggtgtaa gcttttctgc gtgcaacgat tggtaaaagc gctgcaaagt
     2221 accgcgataa aactgtgctt tcaaatcaga agctgaagac atgtcctctt ccggaatgat
     2281 aaaataacat gcatcaatgt cttctttttg ttgataaata tattcaaata atggattgtg
     2341 ttccattcga aacattcgat ttaataacac agcaattgcc ataaacaaaa cacccttcaa
     2401 ttatcttttt attttattaa gctttttgtc actttaaaat aacctagatt cttatttata
     2461 tttttaatta aagcaaacct aaccatttcc aataagtcat gctaaaaact atcactagaa
     2521 tataccctac aattgtcaat ggtatacctg ccttcaagaa atctttaacc gtaaaagtac
     2581 cagtgccata agccaacata ttttgaggtg cactaacagg taataagaaa ccaaaactaa
     2641 taacaaattg ttgaattaaa acaaatccta tagactggtc tcctaagtgt aacgtagagg
     2701 ttagcgaaat aaaaacaggt attaacgctg atgataaact tgttgcactc gcaaagccca
     2761 aatgaatcaa tatattaaaa agcgtgataa gcgcaattgt cgcgataata ggtaaatgtt
     2821 ttaaacctaa aacaccaaaa gtttgatcac ttaaccattg agctgcacct gttttcaaaa
     2881 gaacgttacc tagtgaaata cctacaccaa acacgataat tgttccccat ggtattttat
     2941 tttcaacatg tttccatgtc atgacaccaa ttttcggcat taacataaca cctaaagcaa
     3001 taatagtaat ggatgcagag tcaatcggat gtaatacttt ttcagttgac caaaacagta
     3061 ataataacat cgatataaca attaaacgcc attcacgtgg gctaacgggg ccaagtttat
     3121 gcaattcttc ttttattaaa tctttaccac cttctattgt attaatttct ggaggcatca
     3181 ctttaatcat gatgaaatat aaagctacgg acataactat ggaccaaggc gctgcatata
     3241 agaaccactc gccccatgaa acatcaaatc ctaattgatg gtttataaaa ttaatcgcta
     3301 cgatattttg tgctgccgcc gttttgatac caatattcca aattgacaca gcttgtactg
     3361 aagtaattat taataaagac gctaacttgc tatctttgga aactttaaat gccgcaatca
     3421 tacccagcaa gattggtaca actgcccctg ctctagctgt tgcagaagga acgaaaaatg
     3481 caagtacaat tgaaacgata attgctccaa taactatatt tctagtttta ttaccaacaa
     3541 ttgataacac taaaagagct agtcttttat gcaaattcgt ttcttgcata gcagcagcca
     3601 aaaataatgc agctgcaacg agagctacag ctgaagttgc aaatccacta aacgctaatg
     3661 ataatgcatg attagttcct agaaggtcac ttccagctaa aatagcactg ccacttttcg
     3721 gattacctag cttctcccct aaattttgaa caggggctaa atcctaaaag taatatcatt
     3781 aagccaataa ttaaagttgc tgacaccgga tatgatacag cttccgttac ccacataata
     3841 actgcaaaag ctaaaatagc tagtactgcc ttagccatta tcggcagact tgaaggtgcc
     3901 gggagtaata gtacaactat aagtattata aaactcatga caatccaaaa tggcttgtat
     3961 tgcttttttg tcttttcttt cttttgttgt tcatgttcaa ctacatgttt ttcttctgac
     4021 ataacagtca cccctttgtt taatcttaat ttaattataa caatttttta aaacatacat
     4081 ttttaattta tgcaaaatac acaattttat cgaaatattt tgtaaataat attgctaggt
     4141 gatgagttat ctcggttaat tacaaaacga tatctaagtt taaatattag atcataaaaa
     4201 aactatctac tgaatatcct catagcatca tttttctaag caaaagttaa acttagttag
     4261 ataaaatgca aatgagatta ttgtagatag tctcttttta atgtttaaaa atgattaatg
     4321 ccataaacat aaataattcc aaagaatgcc gcgccactaa gcactgttaa tataagactt
     4381 agtaacaatg tgcgtttata atgtttaaca aatgctatga acaaaatgac tacattatac
     4441 gcaaagaaaa gtccgaataa tgtcattgtt attttcaaat cagaatagaa aatatttaat
     4501 agtaatacaa tgactgcata aattaaatac ggaataacta taaatttgcg ttcaaatctt
     4561 aattgttcga aacgtttatc ttcgtttgtc atgatatgct cctttatgat ttaaaagtaa
     4621 ataagtttac ggaatatctt gtacgataat agcggtgtaa atgtcaaacc acgactgatc
     4681 atctaaatta agttgtagcc cttcgattgc ttgatcaata cgctttaact gacttgttcc
     4741 aagtatcggc atgatacgat gcggtatttt tactaaccac gctatcatca cagctgtgtc
     4801 actcacacca tatttgtcag ctattgattg aacaactttc ataatacgtt gcgctttaat
     4861 atcttccttg tcgaaaattt taccgcctgc aaaaggactc caagccatga tttgaacatg
     4921 gttttgatac attgaatcca ttgttccatc ttgtaaacta tcaacgtgat atggcgataa
     4981 ttctaattga ttgatgctaa tatgtagtct ttctttcata atatattgat ttaacaattg
     5041 gtattgtgaa tgattaaaat tcgacacccc gaatgacttc aacttacctt gtttaacaag
     5101 tttagttaat gcatcagcaa cttgttctgg atccatcaat ggtgaaggac gatgaatgag
     5161 tagactatct aaataatcta cattcaaatt gattaatgac tgttcaacag atttcacgat
     5221 gtgcttacta ctcaaatcat aacgatgtcc atttgtaaaa tcaaattgct tagaaggcaa
     5281 aatgatacca catttcgtaa caatttgaat tttatttctt aattcgggtg ataaatccaa
     5341 agcattacca aacagtgatt cacattgata atctccataa atatcagcat gatccatcgt
     5401 tgtaattcca cgttcaacta attcatttat aaaataattt aactctttcg cagtcatctt
     5461 ccattcattt gcacgccaaa aaccttgtac aagcctagaa aaatgaacat agtgattaat
     5521 cattatttgt tccatatctc atccactcca tccctcaatg tcacaaaata ttcacaataa
     5581 aagtattaag aaattaatgt gctatttccg ttaaattgaa acaacacatt ttctgattta
     5641 cgtaaattgt agcatatcca attgattacg tatgtgattt gtatctaaag tttcaccaat
     5701 aataacaatc gttaatggta attgcatgcc aattattcca tagtctggta aaccatatgc
     5761 atattgaaat tcataaattg catttggttg atctctaaat gacacataac ctttcaaacg
     5821 tagaacagat tccggtaatt tcattataaa ttgataaaac aaatgacgat caatcggacc
     5881 tgtaaacgtg taagtcatac ttttaatccc atgatgatgg tgatgatgag acgatatctc
     5941 tctgtcttta acagttaagt ccaattcttc caacgaaact tttccatagg ttgttttata
     6001 agttggaata cttgcacaaa tcatacctaa ttgcttatca attttctcaa gactgtcatc
     6061 agttataaga tcaattttat taataataat catgtcactt agtttcaact gatcttccat
     6121 cagcgaaact gtattttcgg tatattgatg acgttctaaa aatcgagtcg catctaatac
     6181 accataaata atcggctttt caaagaaatt aacgatttgc ggatcttgac atgcaactag
     6241 taattccaaa ggatgcgcaa tgcctgtcgc ttctatgatg acatgattaa cgtccccttt
     6301 taaagcaatg gcttttagtt catagacaag ttcttgttta agatcgcaac aaacacaacc
     6361 attaatcaat gaatggactt caatttcatt tgaaatacta ttgctatcaa tatcaaaagt
     6421 accgaattca ttcatgatga ttttaatttt
//
LOCUS       DAMQYT010000143         6440 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMQYT010000143 DAMQYT010000000
VERSION     DAMQYT010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.145"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            118..1347
                     /locus_tag="PGA33_002062"
     CDS             118..1347
                     /locus_tag="PGA33_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDG6473863.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTLAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            1542..2399
                     /locus_tag="PGA33_002063"
     CDS             1542..2399
                     /locus_tag="PGA33_002063"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDG6473864.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            2399..3343
                     /locus_tag="PGA33_002064"
     CDS             2399..3343
                     /locus_tag="PGA33_002064"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDG6473865.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            3612..4580
                     /gene="pfkA"
                     /locus_tag="PGA33_002065"
     CDS             3612..4580
                     /gene="pfkA"
                     /locus_tag="PGA33_002065"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDG6473866.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            4602..6359
                     /gene="pyk"
                     /locus_tag="PGA33_002066"
     CDS             4602..6359
                     /gene="pyk"
                     /locus_tag="PGA33_002066"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDG6473867.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
BASE COUNT         2279 a          871 c         1324 g         1966 t
ORIGIN      
        1 aatcaaaacc ataaattctt actcattcag atttacccaa tatttttact tttataatgt
       61 aatgcgtttt atccaagtta ttttttaaaa ataaatattg aattaggggc tgatttcatg
      121 tcattaagag atgaagcatt ggaaatgcac aaacgtaatc aaggtaaatt agaagttaaa
      181 ccaaatgtaa aagttactaa taaagaggaa ttaagtttag catactcacc tggcgttgct
      241 gaaccgtgta aagatattta tgaagataaa agaaaagtat atgattacac aattaaagga
      301 aatacagttg cagttattac tgatggaaca gcggtattag gtttaggtaa cattggacct
      361 gaagcaagta ttcctgtaat ggaaggtaaa gcagtattat tcaaaagctt cgctggtatc
      421 aatggggtgc ctattgcgtt aaatacaact gataccgaag aaatcattaa aacagttaag
      481 ttgttagaac ctaattatgg tggtattaat ttagaggata tttcggcacc acgttgtttt
      541 gaaattgaag aacgattgaa aaaagaaact aatattccgg tattccatga cgatcaacat
      601 ggtacagcaa ttgtaacatt ggcaggtttg gtaaatgcat tgagagttgt taacaaagat
      661 attgctaaaa taaaagttgt actaaatggt gctggtgcag caggaatagc cattgttaaa
      721 ttactatacg cgtatggtgt aagaaatatg gttatgtgtg actcaagagg cgcaattttt
      781 gaaggacgtt catatggtat gaatcctacg aaagatgttg tagcaaaatg gacaaataaa
      841 gataagattg aagggtcttt agaagaagtc gtaaaagatg cagatgtatt tatcggggtt
      901 tctgtagcta atgcgctgtc acaagatatg gttaagagta tggcagataa tccaattata
      961 tttgcaatgg ctaatccaaa tcctgaaata atacctgatg atgccaaagc ggcaggtgca
     1021 cgagttgttg gtacaggacg ttcagactat cctaaccaaa ttaataatgt attagctttc
     1081 cctggtattt ttagaggtgc attagaggtt gaagctacac atataaatga agaaatgaaa
     1141 aaggcagctg tagaagcgat tgctgattta atcgatagtt ctgaattaaa tgaagactac
     1201 tgtatcccag gaccgtttga taaacgtgta gcgccatcag ttgctcgtaa tgttgctaaa
     1261 gcggcaatgg aatctggagt agctaggatt gaagttgatc cgcaagatgt gtatgataaa
     1321 acaatgaaac ttacagattt acaataaaaa taattaacgt ttaattaaat tgcttaaaag
     1381 ttagcaataa agagtcatta aaatgatgaa aatgaaagtt tatgataaac attcaacagt
     1441 caaacgaata taaatcaaat aaatttaaac ccgtttttaa ctggtcaagt tcagtttaag
     1501 gctctaaatg gttagaacag aggttatttg gaggttttcc tatgtttaaa gattttttta
     1561 atcgaacaaa gaaaaagaaa tatcttacag tacaagactc taaaaataat gatgtgcctg
     1621 caggtattat gactaagtgt ccaaagtgta agaaaattat gtacacaaaa gaattagctg
     1681 aaaatttaaa tgtgtgcttt aattgtgatc atcatattgc tttaactgcg tataaacgta
     1741 tagaagcaat ttctgatgaa ggatcattta cagaattcga taagggaatg acctctgcga
     1801 atccattaga ttttccaagt tatttagaaa aaattgaaaa ggaccaacaa aagacaggtc
     1861 ttaaagaagc agttgtgact ggtacagcac aactagatgg tatgaaattt ggcgttgctg
     1921 tcatggattc acgttttaga atgggaagta tgggatcggt tatcggtgaa aagatatgtc
     1981 gcatcattga ttactgcact gagaaccgtt taccatttat tcttttctct gcaagtggtg
     2041 gtgcacgtat gcaagaaggt attatttcct tgatgcaaat gggtaaaacc agtgtatctt
     2101 taaaacgtca ttctgacgct ggactattat atatatcata tttaacacat ccaactactg
     2161 gtggtgtatc tgcaagtttt gcatcagttg gtgatataaa tttaagtgag ccaaaagcgt
     2221 tgataggttt tgcaggtcgt cgagttattg aacagacaat aaacgaaaaa ttgccagatg
     2281 atttccaaac tgcagaattt ttattagagc atggacaatt ggataaagtt gtacatcgta
     2341 atgatatgcg tcaaacattg tctgaaattc taaaaatcca tcaagaggtg actaaataat
     2401 gttagatttt gaaaaaccac tttttgaaat tcgaaataaa attgaatctt taaaagaatc
     2461 tcaagataaa aatgatgtgg atttacaaga agaaattgac atgcttgaag cgtcattgga
     2521 acgagaaact aaaaaaatat atacaaatct aaaaccatgg gatcgtgtgc aaattgcgcg
     2581 tttgcaagaa agacctacga ccctagatta tattccatat atctttgatt cgtttatgga
     2641 actacatggt gatcgtaatt ttagagatga tccagcaatg attggtggta ttggcttttt
     2701 aaatggtcgt gctgttacag ttattggaca acaacgtgga aaagatacaa aagataatat
     2761 ttatcgaaat tttggtatgg cgcatccaga aggttatcga aaagcattac gtttaatgaa
     2821 acaagctgaa aaattcaatc gtcctatctt tacatttata gatacaaaag gtgcatatcc
     2881 tggtaaagct gctgaagaac gtggacaaag tgaatctatc gcaacaaatt tgattgagat
     2941 ggcttcatta aaagtaccag ttattgcgat tgtcattggt gaaggtggca gtggaggtgc
     3001 tctaggtatt ggtattgcca ataaagtatt gatgttagag aatagtactt actctgttat
     3061 atctcctgaa ggtgcagcgg cattattatg gaaagacagt aatttggcta aaattgcagc
     3121 tgaaacaatg aaaattactg cccatgatat taagcaatta ggtattatag atgatgtcat
     3181 ttctgaacca cttggcggtg cacataaaga tattgaacag caagctttag ctattaaatc
     3241 agcgtttgtt gcacagttag attcacttga gtcattatca cgtgatgaaa ttgctaatga
     3301 tcgctttgaa aaattcagaa atatcggttc ttatatagaa taatcaactt gagcattttt
     3361 atgttaaatc gatactgggt tttaccataa attgaagtac attaaaacaa taatttaata
     3421 tttagatact gaattttaac taagattagt agtcaaaatt gtggctacta atcttttttt
     3481 aattaagtta aaataaaatt caatatttaa aacgtttaca tcaattcaat acattagttt
     3541 tgatggaatg acatatcaat ttgtggtaat ttagagttaa agataaatca gttatagaaa
     3601 ggtatgtcgt catgaagaaa attgcagttt taactagtgg tggagattca cctggaatga
     3661 atgctgccgt aagagcagtt gttcgtacag caatttacaa tgaaattgaa gtttatggtg
     3721 tgtatcatgg ttaccaagga ttgttaaatg atgatattca taaacttgaa ttaggatcag
     3781 ttggggatac gattcagcgt ggaggtacat tcttgtattc agcaagatgt ccagagttta
     3841 aggagcaaga agtacgtaaa gttgcaatcg aaaacttacg taaaagaggg attgagggcc
     3901 ttgtagttat tggtggtgac ggtagttatc gcggtgcaca acgcatcagt gaggaatgta
     3961 aagaaattca aactatcggt attcctggta cgattgacaa tgatatcaat ggtactgatt
     4021 ttacaattgg atttgacaca gcattaaata cgattattgg cttagtcgac aaaattagag
     4081 atactgcgtc aagtcacgca cgaacattta tcattgaagc aatgggccgt gattgtggag
     4141 atctagcatt atgggctgga ttatcagttg gtgctgagac aattgtagtt ccagaagtga
     4201 aaacagatat taaagaaata gctgataaaa ttgaacaagg tattaaacgt ggtaagaaac
     4261 actcaatcgt tcttgtagca gaaggttgta tgactgcgca agattgtcaa aaagaattat
     4321 cacaatacat caatgttgat aatagagtgt ctgtgttagg tcacgttcaa cgtggtggta
     4381 gcccaacagg tgcggataga gttttagcat cacgtttagg tggatatgcg gtagacttat
     4441 taatgcaagg tgaaacagct aagggtgttg gaattaagaa caataaaatt gtagcaacat
     4501 cttttgatga aatttttgat ggtaaagatc ataaatttga ttatagtcta tatgaacttg
     4561 ctaacaagtt atctatataa gatttcagga ggaattataa aatgagaaaa actaaaattg
     4621 tatgtacaat tggaccagct tcagaatcag aagaaatgat tgagaaatta atcaatgctg
     4681 gtatgaacgt tgcacgatta aacttttcac atggtagtca tgaagagcat aaaggtagaa
     4741 ttgatacaat tcgtaaagta gctaaaagat tagacaaaat tgtagcaatt ttattagata
     4801 caaaaggtcc agaaattcgt acgcataata tgaaagacgg tatcattgaa cttgaacgtg
     4861 gcaacgaagt tattgttagc atgaatgaag ttgaaggaac acctgaaaag ttctcagtaa
     4921 catatgaaaa cttaattaac gatgttcaag taggttcata cattttactt gatgatggct
     4981 taattgaatt acaagttaaa gatattgacc atgctaaaaa agaagttaaa tgtgatattt
     5041 taaactctgg tgagcttaaa aacaaaaaag gtgttaactt acctggcgta agagtaagtt
     5101 tacctggtat tacagaaaaa gatgctgaag atatccgttt cggtattaaa gaaaatgttg
     5161 acttcattgc agcaagtttc gtacgtcgtc ctagtgatgt tttagaaatt cgtgaaattt
     5221 tagaagaaca aaaagctaac atttcagtat tccctaaaat tgaaaaccaa gaaggtattg
     5281 ataatattgc ggaaattctt gaagtgtctg atggtttaat ggttgcacgt ggtgacatgg
     5341 gtgttgaaat tccacctgaa aaagtaccaa tggttcaaaa agatttaatc agacaatgta
     5401 acaaattagg taaaccagtt attacagcta cacaaatgtt agattctatg caacgtaacc
     5461 cacgtgctac acgtgcagaa gctagtgacg ttgccaacgc aatctatgat ggtacagatg
     5521 cagtaatgtt atctggtgaa actgctgctg gtttatatcc tgaagaagct gttaaaacaa
     5581 tgagaaatat tgctgtatca gctgaagcag cccaagatta caaaaagtta ttgtcagatc
     5641 gtactaaatt agttgaaact tcattagtga atgctatcgg tatttcggtt gcacatacag
     5701 ctttaaactt aaatgttaaa gcaattgtag ctgctactga aagtggttca acggcacgta
     5761 ctatctccaa atatcgtcca cattcagaca ttattgcggt gactccaagt gaagaaactg
     5821 cacgtcaatg ttcaattgtt tggggagttc aacctgtagt taaaaaagga cgtaagagta
     5881 cagatgcatt gttaaacaat gcagttgcaa cagctgttga aactggtaga gtatctaatg
     5941 gtgatttaat cattattact gctggtgtac caactggtga aactggaact actaatatga
     6001 tgaaaatcca cctagttggt gacgaaattg ctaatggtca aggtattgga cgtggatcag
     6061 ttgttggtac tacgttagtt gctgaaactg ttaaagattt agaaggtaaa gatttatctg
     6121 acaaagttat cgttactaac tcaatcgatg aaacgtttgt accttatgta gaaaaagctt
     6181 taggcttaat tacagaagaa aatggtatta catcaccaag tgcaattgtt ggtttagaaa
     6241 aaggtattcc aacagttgta ggtgtagaaa aagctgttaa aaacataagc aataacatgt
     6301 tagttacgat tgatgctgct caaggtaaaa tctttgaagg atatgcaaac gtactataat
     6361 ttataaaaaa acgtctttcc atttatcaac aatggaaagg cgttttttgg ttcatctggt
     6421 attttatgac gtaattaata
//
LOCUS       DAMQYT010000144         6395 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMQYT010000144 DAMQYT010000000
VERSION     DAMQYT010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.146"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(41..277)
                     /locus_tag="PGA33_002067"
     CDS             complement(41..277)
                     /locus_tag="PGA33_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HDG6473868.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            complement(274..924)
                     /locus_tag="PGA33_002068"
     CDS             complement(274..924)
                     /locus_tag="PGA33_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDG6473869.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            complement(1098..1937)
                     /locus_tag="PGA33_002069"
     CDS             complement(1098..1937)
                     /locus_tag="PGA33_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDG6473870.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKSPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVTKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            complement(2085..2192)
                     /locus_tag="PGA33_002070"
     CDS             complement(2085..2192)
                     /locus_tag="PGA33_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473871.1"
                     /translation="MCLLKQHMQCAFSNITYGDFQGIELIIENVLNYMV"
     gene            complement(2346..3456)
                     /gene="prfB"
                     /locus_tag="PGA33_002071"
     CDS             complement(join(2346..3383,3385..3456))
                     /gene="prfB"
                     /locus_tag="PGA33_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDG6473872.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            complement(3625..3729)
                     /locus_tag="PGA33_002072"
     CDS             complement(3625..3729)
                     /locus_tag="PGA33_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473873.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKKSV"
     gene            complement(3771..6302)
                     /gene="secA"
                     /locus_tag="PGA33_002073"
     CDS             complement(3771..6302)
                     /gene="secA"
                     /locus_tag="PGA33_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDG6473874.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
BASE COUNT         1895 a         1209 c          889 g         2402 t
ORIGIN      
        1 tttcaacaaa tttgtttgaa acattatttt aaagttaatc ttagcgatct tcatcttgat
       61 gtttatgaaa ttcgagttga tctataatta aataaccagc taataatgac actacatcaa
      121 taaaaataat ccactcgtta tggaaatact ctttatagat tgaggcacca attaaaatta
      181 atgtcagaat agtaccgacc catttacttc ttgttattac actaaataat actaccaaga
      241 cacatggaaa gaatgctgcg ctaaaatacc atatcattca ttttcctctt ttcttttatt
      301 taaaatgttc atggttgttt ctcttaattc tgttctaggt ataaagtttt cagtcaacat
      361 ttctggaatg atattattaa taaaatcttg tacagatgct aaatggtcaa attgaataat
      421 tgtttctaga ctcatttcat aaatttcgaa aaataattct tcgggattac gtttttgtat
      481 ttctccaaat gtttcataaa gcaaatcaat tttatcagca actgaaagta tttggccttc
      541 taatgaatca tctttacctt cttgcagtcg ttgcttataa acatctctat attgtaatgg
      601 aatttcttct tcaataaagg tctctaccat ttcttcttca acttgcgaaa ataatttttt
      661 taattcacta ctcgcatatt taacaggtgt ttttatatca ccagtaaaca cttcggcgaa
      721 atcatgattt aatgcttttt catataagct tttccaatta atctttcgtc catgatattc
      781 ttcaactgtt gctagatatt gtgcaatttt agttacttta aaggagtgtg ctgcaacatt
      841 gtgttcaaaa tatttaaatt ttccaggtaa tcttataagt ctttccatat ctgataatct
      901 tttaaaatat tgatgtacac ccatttcaat tacctcctcc attaattaat cataaattat
      961 actttctttt tacatatcaa tcaattaaat atcatttaaa tatcttcttt atataactct
     1021 gattaaatga taccaaaaaa tcctctcaac ctgttactta aacaggctaa gaggatagtc
     1081 ttgtcttgat atattactta gtggatgtaa ttatattttc ctggatttaa aattgttctt
     1141 gaagatttaa cattaaatcc agcatagttc atttcagaaa cagtaattgt tccattaggg
     1201 tttacagatt caacaacacc aacatgtcca tatggaccag cagctgtttg gaaaatagcg
     1261 ccaacttctg gtgttttatc tactttaaat cctgcaactt ttgctgcgta attccagtta
     1321 tttgcattgc cccataaact tcctatactt ctacctaatt gtgcacgacg atcgaaagca
     1381 taatatgtgc agtttccata agcatataag tttcctctgt tagcaactga tttattgtag
     1441 ttatgtgcaa caggtacagt tggtactgat ttttgtactt gagcaggttt gtatgctaca
     1501 ttaactgtct tagttactgc ttgcttaggt gcttgcttaa ctactacttt tttagatgct
     1561 tgttgtacag gttgttttac taccttttta gcttggcttg cttttcttac tggtgattta
     1621 accgctttag tttgtttcac tttattttga ggcacaagtg aaatcacgtc accaggaaaa
     1681 attaaaggtg ttacaccagg attgtatgaa tataattgat tcaacgttaa gtgatgctct
     1741 aaagcaatct tatataatga atcgccagca actactgtat aagttgtcgg tgattgcgtt
     1801 tgtgcttgaa catttgatac ataattatgt tgaacaggtg tttttacttg tgtgccatgt
     1861 tgttgtgcat gtgctgcatt atttaaagct aaaaaagcta acactgacga aaccgtcact
     1921 gtaagagatt ttttcatctt gctgtcattc ctttgctgtt agtattttaa gtatgcaaat
     1981 actatagcac aatacatttt gtcaaaagct attgttataa cgatgtaatc aaatggttaa
     2041 caatataaaa agaatacaac cttttatcat agtgtaaaat gtattcatac catgtaattg
     2101 agaacgtttt caataattaa ttcaatacct tgaaaatcgc cataggtaat attactaaat
     2161 gcacactgca tatgttgttt taacaaacac aacttttaaa aaatatattc taactctatc
     2221 taccgaattg tacttaaata ttcataaaca aatcatattc caaaatctaa tttacaattt
     2281 atttagctac ctttaaaaaa accaaaaacc gacgcccttt tagagcctcg gttttaaaat
     2341 atatattaat cgtgcgacat tgtctgtctt aaatatgatt cgataaatgg tccaatgtct
     2401 ccatccatca ctgcatcaac cttacctgtt tcttcgttcg tacgatgatc tttcaccatt
     2461 gagtatggat ggaaaacata tgatctaatt tggcttcccc agccgatttc tttttgttcg
     2521 ccacgaattt cagccatttc acgtgcctgc tcttccaatt ttaattgata taatttagac
     2581 tttaacattt tcatagctgc ttcacggttt ttaatttgag aacgttcatt ttggttatta
     2641 acaactatac ctgaggggtg gtgggtaatt cgtattgccg attcagtttt gttaatatgc
     2701 tgaccacctg caccagaagc tctgaatgta tcaactgtaa tatcatccgg attgatttca
     2761 atctctattt catcattatt aaaatctgga ataacgtcgc atgatgcaaa tgatgtatga
     2821 cgacgtcctg atgaatcaaa tggagaaatt cgtactagtc ggtgtacacc tttttcagct
     2881 tttaaataac cataagcatt atgccctttg atgagcaatg ttacactttt aatccccgct
     2941 tcatccccag gtagataatc aacagtttca actttaaagc ctttcttctc acaataacgt
     3001 tgatacattc taaatagcat attagcccaa tcttgagact ccgtgccacc tgcaccagga
     3061 tgtaactcta gaattgcgtt attggcatcg tgaggcccat ctaataataa ttgcaattcg
     3121 tattcatcca ctttagcctt aaaattaatg acctcttgct ctaagtcttc tttcatttct
     3181 tcatcaaatt cttcttgtaa taaatcccaa gtagcatcca tgtcatctac ttctgcttgt
     3241 agtgttttat aaccattaac tattgctttt aacgcattat ttttatctat aatatcttgc
     3301 gctttcgttt ggttatccca aaaattaggt tctgccatca tttcttcata ttcttgaata
     3361 ttagtttctt tgttctctaa gtcaaagaga ccccctaatt tgtgttaaat cttgattata
     3421 cttatctata tttcgtttga tttctgataa ttccatagca ttcgctccta tttatatttc
     3481 aattcaagtc attgatttgc atcttttata atgctaaatt ttaacataat tttgttaaat
     3541 aacaatgtta agaaatataa gcacactgac aattagttta tgcatttatt gttaaaaatc
     3601 agtacattta tcatcgacat atgcctaaac cgatttttta aaactaagta cataacaacg
     3661 tttaacaact tcttcacatt ttttaaagta tttaacgctt gtaaaataaa aagactcctc
     3721 ccataacaca aactataggt gtttaattgg aaggagttat tttatatcat ttattttcca
     3781 tggcaatttt tgaatttttt accactacca catggacaat catcgttacg accaacttga
     3841 tcgcctttaa cgattggttt cggtttcact ttttctttac catcttcagc tgaaacgtgc
     3901 ttcgcttcac caaactctgt tgttttttca cgttcaatat tatcttcaac ttgtactaca
     3961 gattttaaaa tgaatttaca agtatcttct tcaatatttt gcatcatgat atcaaataat
     4021 tcatgacctt cattttgata gtcacgtaat ggattttgtt gtgcataaga acgtaagtga
     4081 ataccttgac gtaattgatc cattgtgtcg atatgatcag tccaatggct atcaatagaa
     4141 cgaagtaaaa tcatacgctc aaactcattc atttgttctt ctaagatatc tttttgactt
     4201 tgatatgctg cttcaatctt agcccaaacg acttcgaaaa tatcttcagc atctttacct
     4261 ttgatatcat cctctgtaat gtcaccttct tgtaagaaga tgtcattaat gtagtcgatg
     4321 aatggttgat attcaggctc gtcatctgct gtattaatat agtaattgat actacgttgt
     4381 aacgttgaac gtagcattgc atctacaact tgagagctgt cttcttcatc aataatacta
     4441 tttctttcgt tatagataat ttcacgttgt ttacgtaata cttcatcgta ttctaagata
     4501 cgtttacgcg cgtcgaagtt attaccttct acacgttttt gtgctgattc tacagctctt
     4561 gataccattt ttgattcaat tggtgtagag tcatctaaac ctagtcggct catcattttc
     4621 tgtaaacgtt cagaaccaaa acgaatcatt aattcatctt gtaatgataa atagaagcga
     4681 ctatcccctt tatcaccttg acgtccagaa cgaccacgta actggtcatc aatacgacga
     4741 gattcatgtc gctctgtacc tattactgct aaaccgccta attcctctac gccttcacct
     4801 aatttgatat ctgtaccacg accagccatg ttagtggcaa tagtaacggc acctttttgt
     4861 ccagcgcctg caacaatttc agcttcacgt tcatgatttt tcgcatttaa cacatcatga
     4921 cggataccac gttttttaag taaatttgaa atatattcag aagtctcaac tgcaacagta
     4981 cctaatagca ctggttgccc tgccttgtgt ttttcaacaa catcttctac tactgcatca
     5041 aatttacctt tttggctaat gtaaattaaa tcagacttat cgttacgttg cacaggttta
     5101 tttgtcggaa tttgagttac tgtcatgtta taaatatttc taaattcttc ttcttcagtt
     5161 ttagctgtac ctgtcatacc cgcaagttta ttgtacattc tgaaatagtt ttggaatgta
     5221 atagacgcca tagttttaga ttcattttga atttgaacgc cttccttcgc ttcaatagct
     5281 tggtgtaaac cttccgagaa acgacggcct ggcattgtac gtcctgtaaa ttgatcgaca
     5341 attaatactt cgccatcaac aaccatatag tctacgtcac gttgtaatgt aacgtgcgca
     5401 cgtaaagctg tgttgatatg actaataaca tcaacatttt gtacatcata taagttttca
     5461 actttgaaca tacgttcagc tttatccgca ccttgttctg ttaaatgtac agctttcgtt
     5521 ttttcatcgt atttataatc ttcgtcctgt tttaacattt tcgcaaaaac atttgcttgt
     5581 gtataaagtg acgttgactt ttcagcttca ccagaaataa ttaatggcgt acgtgcctcg
     5641 tcgattaaaa ttgagtcaac ctcatcaatg attgcaaaat gtaatggacg cattactcta
     5701 tcttcagaat aattcaccat gttatctcgt aagtaatcaa aacctagctc attattagta
     5761 ctgtaagtaa tgtcttgtgc gtatgcttca cgtttttctt ctgtcgtctt actgtttaag
     5821 tttaatccga cagtcaaacc taagaagtta tataactcag ccatttcttc actttgaaca
     5881 cttgataagt attcattgac tgtaataacg tgaacacctc taccagctaa tgcatttaag
     5941 tatgttggca ttgtcgctgt taatgtttta ccttcacctg ttctcatctc agcgatatca
     6001 cctttatgaa ttgcaatacc acccataatt tgaactttat atggtgtcat attgaataca
     6061 cgtttagagc cttctctaac aagtgcatat gcttctggta aaattttatc taaataatca
     6121 ttttgctttt tgacattatc aatgtcagct aattctgttt ggaattgttt cgttttatta
     6181 cgaatttctt catcagttaa aattgccgtt ttttcttcta aagcgattac tttatcagca
     6241 agtttaccta actgtttaat ttctttatta ttgccatcaa gaatttttga taaaaatccc
     6301 atttcgttcg ctcctttagc taaaaaactg tttggcctac aacaatatat cttatcattt
     6361 atagttagaa aattatactt atttactcat ttgta
//
LOCUS       DAMQYT010000145         6358 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMQYT010000145 DAMQYT010000000
VERSION     DAMQYT010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.147"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..747
                     /gene="codY"
                     /locus_tag="PGA33_002074"
     CDS             <1..747
                     /gene="codY"
                     /locus_tag="PGA33_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDG6473875.1"
                     /translation="LNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSRRGKILGSSL
                     NELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPENRELFIDSRT
                     TIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHSEVEKEARDK
                     AAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIVNALRKLESA
                     GVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            798..890
                     /locus_tag="PGA33_002075"
     CDS             798..890
                     /locus_tag="PGA33_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473876.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDISH"
     gene            1089..1856
                     /gene="rpsB"
                     /locus_tag="PGA33_002076"
     CDS             1089..1856
                     /gene="rpsB"
                     /locus_tag="PGA33_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDG6473877.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            1890..2003
                     /locus_tag="PGA33_002077"
     CDS             1890..2003
                     /locus_tag="PGA33_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473878.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            2038..2919
                     /locus_tag="PGA33_002078"
     CDS             2038..2919
                     /locus_tag="PGA33_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDG6473879.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            3056..3778
                     /locus_tag="PGA33_002079"
     CDS             3056..3778
                     /locus_tag="PGA33_002079"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDG6473880.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            3797..4351
                     /gene="frr"
                     /locus_tag="PGA33_002080"
     CDS             3797..4351
                     /gene="frr"
                     /locus_tag="PGA33_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDG6473881.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
     gene            4724..5494
                     /locus_tag="PGA33_002081"
     CDS             4724..5494
                     /locus_tag="PGA33_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDG6473882.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            5501..6283
                     /locus_tag="PGA33_002082"
     CDS             5501..6283
                     /locus_tag="PGA33_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDG6473883.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
BASE COUNT         2358 a          851 c         1235 g         1914 t
ORIGIN      
        1 ttaaacacgt tacttcaaaa acacaaaggt attgcggttg attttaaaga tgtagcacaa
       61 acgattagta gcgtaactgt aacaaatgta tttattgtat cgcgtcgagg taaaatttta
      121 ggatcgagtc taaatgaatt attaaaaagt caaagaatta ttcaaatgtt ggaagaaaga
      181 catattccaa gtgaatatac agaacgatta atggaagtta aacaaacaga atcaaatatt
      241 gatatcgaca atgtattaac agtattccca cctgaaaaca gagaattatt catagatagt
      301 cgtacaacta tcttcccaat tttaggtgga ggggaaagat taggtacatt agtacttggt
      361 cgagtacatg atgattttaa tgaaaatgat ttggtactag gtgaatatgc tgctacagtt
      421 attggtatgg aaatcttacg tgagaagcat agtgaagtag aaaaagaagc gcgcgataaa
      481 gctgctatta caatggcaat taattcatta tcttattctg aaaaagaagc gattgaacat
      541 atctttgaag aacttggcgg tacggaaggc ctattaatcg catcaaaagt tgcagataga
      601 gttggtatta ctagatctgt aattgtaaat gcactacgta aattagaaag tgctggtgta
      661 attgaatcac gttctttagg aatgaaaggt actttcatta aagttaaaaa agaaaaattc
      721 ttagatgaat tagaaaaaag taaataagtc gatgagtctg ggacataatt caatgtccta
      781 gtcgtgacga tgttatcttg gcggtagtca atgaaatgac tttgatgatt atgttgaagt
      841 catttcttta tttttgtcta attttcaatc aaattgatat ttcacattaa cttgatagaa
      901 ttacctttaa atttatggaa aaatgcttga aaaacatttg ctttagacac ggcattatgt
      961 tatatttatt ttcggctata aagccaatac acacatatca aacgtgattg tgtaaagggt
     1021 gcaatattta tatattgtct ttacaatagt ttgctatgga ggtaattaac caataggagg
     1081 aatttataat ggcagtaatt tcaatgaaac aattactaga agcgggtgtt cacttcggtc
     1141 accaaacacg tcgttggaac ccaaaaatga aaaaatatat cttcactgag agaaatggta
     1201 tttatatcat cgacttacaa aaaacagtga aaaaagtaga cgaggcatac aacttcttga
     1261 aacaagtttc agaagatggt ggacaagtct tattcgtagg aactaaaaaa caagcacaag
     1321 aatcagttaa atctgaagca gaacgtgctg gtcaattcta cattaaccaa agatggttag
     1381 gtggattatt aactaactat aaaacgatct caaaacgaat caaacgtatt tctgaaattg
     1441 aaaaaatgga agaagatggt ttattcgaag tattacctaa aaaagaagta gtagaactta
     1501 aaaaagaata cgaccgttta atcaaattct taggcggaat tcgtgatatg aaatcaatgc
     1561 ctcaagcatt attcgtagtt gacccacgta aagagcgtaa tgcaattgct gaagctcgta
     1621 aattaaatat tcctatcgta ggtatcgttg acactaactg tgatcctgac gaaattgact
     1681 acgttatccc agcaaacgac gatgctatcc gtgcggttaa attattaact gctaaaatgg
     1741 cagatgcaat cttagaaggt caacaaggcg tttctaatga agaagtagct gcagaacaaa
     1801 acatcgattt agatgaaaaa gaaaaatcag aagaaacaga agcaactgaa gaataatcaa
     1861 ctgttgaatc tgacttagat atagtttaaa tgggtgataa gatattaatg cttatcacct
     1921 tttttaaaaa gaaaatcgag gcaaattaca aatattcaat tagagtattg gcaatcttgc
     1981 ctataataat gctaaaatca taatatataa aatgataact tattggagga ataatgaatg
     2041 gcaactattt cagcaaaact tgttaaagaa ttacgtgaaa aaactggcgc gggtatgatg
     2101 gattgtaaaa aagcgctaac tgaaactgat ggtgacatcg ataaagcgat tgactaccta
     2161 cgtgaaaaag gtattgctaa agcagctaaa aaagcagacc gtattgcggc tgaaggttta
     2221 gtacatgtag aaactaaagg taacgacgca gttatcgttg aaatcaactc tgaaacagac
     2281 tttgttgctc gtaacgaagg tttccaagag ttagttaaag aaatcgctaa tcaagtatta
     2341 gatacaaaag ctgaaactgt tgaagcttta atggaaacaa ctttaccaaa tggtaaatca
     2401 gttgatgaaa gaattaaaga agcaatttca acaatcggtg aaaaattaag tgttcgtcgt
     2461 tttgctatca gaactaaaac tgataacgat gctttcggcg cttacttaca catgggtgga
     2521 cgcattggtg tattaacagt tgttgaaggt tcaactgacg aagaagcagc aagagacgtt
     2581 gctatgcata tcgctgcaat caaccctaaa tatgtttctt ctgaacaagt tagcgaagaa
     2641 gaaatcaacc acgaaagaga agttttaaaa caacaagcat taaatgaagg taaaccagaa
     2701 aacatcgttg aaaaaatggt ggaaggacgt ttacgtaaat acttacaaga aatttgtgct
     2761 gtagatcaag acttcgttaa aaaccctgat gtaacagttg aagctttctt aaaaacaaaa
     2821 ggtggaaaac ttgttgactt cgtacgctat gaagtaggcg aaggtatgga aaaacgcgaa
     2881 gaaaactttg cggatgaagt taaaggacaa atgaaataat ctgtcataaa gtaaaacaag
     2941 gaagaagaca cctttaatgt tgctttatta aaatgtaaat cattctaata aaacgacaac
     3001 tgtgtcttct ttacttgtat atgttacata tattcacgat agagaggata agaaaatggc
     3061 tcaaatttct aaatataaac gtgtagtttt gaaactaagt ggtgaagcgt tagctggaga
     3121 aaaaggattt ggcataaatc cagtaattat taaaagtgtt gctgagcaag tggctgaagt
     3181 tgctaaaatg gactgtgaaa tcgcagtaat cgttggtggc ggaaacattt ggagaggtaa
     3241 aacaggtagt gacttaggta tggaccgtgg aactgctgat tacatgggta tgcttgcaac
     3301 tgtaatgaat gccttagcat tacaagatag tttagaacaa ttggattgtg atacacgagt
     3361 attaacatct attgaaatga agcaagtggc tgaaccttat attcgtcgtc gtgcaattag
     3421 acacttagaa aagaaacgcg tagttatttt tgctgcaggt attggaaacc catacttctc
     3481 tacagatact acagcggcat tacgtgctgc agaagttgaa gcagatgtta ttttaatggg
     3541 caaaaataat gtagatggtg tatattctgc agatcctaaa gtaaacaaag atgcggtaaa
     3601 atatgaacat ttaacgcata ttcaaatgct tcaagaaggt ttacaagtaa tggattcaac
     3661 agcatcctca ttctgtatgg ataataacat tccgttaact gttttctcta ttatggaaga
     3721 aggaaatatt aaacgtgctg ttatgggtga aaagataggt acgttaatta caaaataaat
     3781 ttagaggtgt aaaataatga gtgacattat taatgaaact aaatcaagaa tgcaaaaatc
     3841 aatcgaaagc ttatcacgtg aattagctaa catcagtgca ggaagagcta attcaaattt
     3901 attaaacggc gtaacagttg attactatgg tgcaccaaca cctgtacaac aattagcaag
     3961 catcaatgtt ccagaagcac gtttacttgt tatttctcca tacgacaaaa cttctgtagc
     4021 tgacatcgaa aaagcgataa tagcagctaa cttaggtgtt aacccaacaa gtgatggtga
     4081 agtgatacgt attgctgtac ctgccttaac agaagaacgt agaaaagagc gcgttaaaga
     4141 tgttaagaaa attggtgaag aagctaaagt atctgttcga aatattcgtc gtgatatgaa
     4201 tgatcagttg aaaaaagatg aaaaaaatgg cgacattact gaagatgagt tgagaagtgg
     4261 tactgaagat gttcagaaag caacagacaa ttcaataaaa gaaattgatc aaatgattgc
     4321 tgataaagaa aaagatatta tgtcagtata aaactaatat acaatgacat attaaaatgc
     4381 cagtattaaa cgataatgta acatttaaaa tgggcatgtt taattaaatc aaagatgcat
     4441 gtgataattt aaattcacaa tgagcataaa aatggtgttt aaacaagtta attaaacata
     4501 tactttataa ataataggca ttaggtatat tgctataata aagttatgta atttttaacc
     4561 tcagtatgta tgtcacattt ctggtgtaaa ctgtaccgag tcagactttg gtacagtttt
     4621 tttatttgct tattcaatgc attaaatgag tatgataaaa tgataatgat tgtttagtaa
     4681 cttatactat atgacagaga tgatcaggct cggaggaaag accatgttta aaaagctaat
     4741 aaataaaaag aacactataa ataattataa tgaagaatta gactcgtcta atatacctga
     4801 acatatcgct attattatgg atggtaatgg gcgatgggct aagaagcgaa aaatgcctag
     4861 aattaaaggt cattacgaag gtatgcaaac aataaaaaaa attactaggg tagctagtga
     4921 tattggtgtt aagtacttaa ctttatacgc cttttccact gaaaattggt caagacctga
     4981 aagtgaagta aattatatta tgaatttgcc tgtcaatttc ttaaagacat tcttaccgga
     5041 actaattgaa aaaaatgtca aagttgaaac aattggattt actgataagt tgccaaaatc
     5101 aacgatagaa gcaattaata atgctaaaga aaagacagct aataataccg gcttaaaatt
     5161 aatatttgca attaattatg gtggcagagc agaacttgtt catagtatta aaaatatgtt
     5221 tgacgagctt catcaacaag gtttaaatag tgatatcata gatgaaacat atataaacaa
     5281 tcatttaatg acaaaagact atcctgatcc agagttgtta attcgtactt caggagaaca
     5341 aagaataagt aatttcttga tttggcaagt ttcgtatagt gaatttatct ttaatcaaaa
     5401 attatggcct gactttgacg aagatgaatt aattaaatgt ataaaaattt atcagtcacg
     5461 tcaaagacgc tttggcggat tgagtgagga gtagtatagt atgaaagtta gaacgctgac
     5521 agctattatt gccttaatcg tattcttgcc tatcttgtta aaaggcggcc ttgtgttaat
     5581 gatatttgct aatatattag cattgattgc attaaaagaa ttgttgaata tgaatatgat
     5641 taaatttgtt tcagttcctg gtttaattag tgcagttggt cttatcatca ttatgttgcc
     5701 acaacatgca gggccatggg tacaagtaat tcaattaaaa agtttaattg caatgagctt
     5761 tattgtatta agttatactg tcttatctaa aaacagattt agttttatgg atgctgcatt
     5821 ttgcttaatg tctgtggctt atgtaggcat tggttttatg ttcttttatg aaacgagatc
     5881 agaaggatta cattacatat tatatgcctt tttaattgtt tggcttacag atacaggggc
     5941 ttacttgttt ggtaaaatga tgggtaaaca taagctttgg ccagtaataa gtccgaataa
     6001 aacaatcgaa ggattcatag gtggcttgtt ctgtagtttg atagtaccac ttgcaatgtt
     6061 atattttgta gatttcaata tgaatgtatg gatattactt ggagtgacat tgattttaag
     6121 tttatttggt caattaggtg atttagtgga atcaggattt aagcgtcatt tcggcgttaa
     6181 agactcaggt cgaatactac ctggacacgg tggtatttta gaccgatttg acagctttat
     6241 gtttgtgtta ccattattaa atattttatt aatacaatct taatgctgag aacaaatcaa
     6301 taaacgtaaa gaggagttgc tgagataatt taatgaatct cagaactcct tttgaaaa
//
LOCUS       DAMQYT010000146         6317 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMQYT010000146 DAMQYT010000000
VERSION     DAMQYT010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.148"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            272..1438
                     /gene="aroC"
                     /locus_tag="PGA33_002083"
     CDS             272..1438
                     /gene="aroC"
                     /locus_tag="PGA33_002083"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDG6473884.1"
                     /translation="MRYLTSGESHGPQLTVIVEGVPANIEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDSIAQAMRDKIDEAKNEGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGKIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNSEIGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLKQQIIERRQLNIEF"
     gene            1464..2528
                     /locus_tag="PGA33_002084"
     CDS             1464..2528
                     /locus_tag="PGA33_002084"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDG6473885.1"
                     /translation="MKLQTTYPSNNYPIYVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFDDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFIAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVIADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHDINHYIQYLIQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            2538..3836
                     /gene="aroA"
                     /locus_tag="PGA33_002085"
     CDS             2538..3836
                     /gene="aroA"
                     /locus_tag="PGA33_002085"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDG6473886.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTT
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            3843..5087
                     /locus_tag="PGA33_002086"
     CDS             3843..5087
                     /locus_tag="PGA33_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473887.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            5101..5676
                     /locus_tag="PGA33_002087"
     CDS             5101..5676
                     /locus_tag="PGA33_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDG6473888.1"
                     /translation="MSETFNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDN"
     gene            5666..6253
                     /locus_tag="PGA33_002088"
     CDS             5666..6253
                     /locus_tag="PGA33_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HDG6473889.1"
                     /translation="MTIKAFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILYLVISISLILIQKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMIWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
BASE COUNT         2241 a          864 c         1182 g         2030 t
ORIGIN      
        1 tgcgatacga ttatattaaa acggctaatc atttttaatt aatgattata tgatgcaact
       61 gtttagaaat tcatgatact tttctacaga cgaatatatt ataattaatt ttagttcgtt
      121 taatattaag ataattctga catttaaaat gagatgtcat ccattttctt aattgagctt
      181 gaaaacaaac atttatgaat gcacaatgaa tatgataaga ttaacaacat attataatgt
      241 tatcgtggaa gtatgaaagg agcgagtgtg tatgagatac ctaacatcag gagaatcaca
      301 tggacctcaa ttaacagtta ttgttgaagg tgtacctgca aatatagaaa ttaaggttga
      361 ggatattaat aaagaaatgt ttaagcgtca aggcggttac ggacgtggac gtcgtatgca
      421 aattgagaaa gatacagtag aaatagtatc aggcgttaga aatggttata cattaggtag
      481 tccaattact atggttgtaa ccaatgatga ctttacgcat tggagaaaaa ttatgggagc
      541 agctccaata agtgaagaag aacgtgaaaa tatgaaacgt actattacaa aaccaagacc
      601 tggtcatgca gatttggttg gaggtatgaa atataatcat cgtgatttac gaaatgtgct
      661 agagcgatca tctgctagag aaacagcagc tcgagttgca gtcggtgcct tatgtaaagt
      721 gttattacaa cagttagata tcgatatata cagtcgtgtt gttgaaatag gtggaattaa
      781 agataaagat ttttatgatt cagaaacatt taaagcaaat cttgatcgta atgatgttcg
      841 tgtaattgat gacagtatcg cacaagcaat gcgagataaa attgacgaag ctaaaaatga
      901 aggagattca attggcggtg tcgttcaagt tgtagttgaa aatatgcctg ttggtgtagg
      961 tagttatgtg cattatgatc gtaagttaga tggtaagatt gcacaaggtg ttgtcagcat
     1021 aaatgctttt aaaggtgtaa gctttggtga aggatttaaa gcagctgaaa agccaggtag
     1081 tgagattcaa gatgaaattc tatataatag tgaaattggt tattatcgtg gatctaatca
     1141 cttaggtggt ttagaaggcg gtatgtcaaa tggaatgcca attatcgtta atggtgtaat
     1201 gaaaccaatt ccaacgttat ataaaccatt aaattcagta gacattaata ctaaagaaga
     1261 ctttaaagca acaattgaac gttctgatag ttgtgctgtt cctgcagcaa gtatcgtctg
     1321 cgaacatgtc gtagcatttg aaatagcaaa agcattattg gaagaattcc aatcaaatca
     1381 tattgagcaa cttaaacaac aaattattga gcgcagacaa ttaaatattg agttttaaca
     1441 acaagaacaa ttgaggtgta atcatgaaat tacaaacaac atacccttca aataattatc
     1501 caatatatgt tgaacacggt gcaattgacc atattagcac gtatattgat cagtttgatc
     1561 aaagttttat attaattgac gagcatgtaa atcaatattt tgctgataaa tttgatgata
     1621 ttttatcata tgaaaatgta cataaagtta ttattccagc tggtgaaaag acgaaaacat
     1681 ttgagcaata tcaagaaaca ttagagtata ttttatccca tcatgtaact cgtaatacag
     1741 caattattgc tgttggtggt ggtgcaactg gtgattttgc tgggtttatt gcggcgacac
     1801 ttttacgagg cgttcacttt atacaagtgc caacgactat actagcgcat gattctagtg
     1861 ttggcggtaa agtgggtatt aactcaaagc aaggtaaaaa ccttatcggt gcattttatc
     1921 gtccaactgc tgtgatttat gatttagact ttttaaagac gttaccattt gagcaaatat
     1981 taagtggcta tgcagaagtt tataagcatg cgttattgaa tggtgaatca gcgacgcaag
     2041 atatcgaaca gcactttaaa gatagagaga tattacagtc attaaatggt atggataaat
     2101 atattgctaa aggtattgaa acgaagctgg atattgttat tgcagatgaa aaagaacaag
     2161 gtgtacgtaa atttttaaat ttaggtcata catttggtca tgctgttgaa tactatcata
     2221 aaatacctca tggtcatgca gtgatggttg gcattatcta tcaatttata gttgcgaatg
     2281 ctttgtttga ttctaagcat gatattaatc attatattca atatttaata caactcggct
     2341 atcctttaga catgataact gacttggatt ttgaaacgtt ataccaatat atgctaagtg
     2401 ataaaaagaa tgataagcaa ggtgtacaaa tggtcttgat tagacaattt ggagatatcg
     2461 ttgtacaaca tgttgatcaa ctaacattac aacatgcatg tgaacaatta aaaacatatt
     2521 ttaagtaggt gaatgaaatg gtaaatgaac aaatcattga tatttcaggt ccgttaaagg
     2581 gcgaaataga agtgccgggc gataagtcaa tgacacaccg tgcaatcatg ttggcgtcgc
     2641 tagctgaagg tgtatctact atatataagc cactacttgg cgaagattgt cgtcgtacga
     2701 tggacatttt ccgactgtta ggtgtagaaa tcaaagaaga tgatgaaaaa ttagttgtga
     2761 cttccccagg atatcaatct tttaacacgc cacatcaagt attgtataca ggtaattctg
     2821 gtacgacaac acgattattg gcaggtttgt taagtggttt aggtattgaa agtgttttgt
     2881 ctggcgatgt ttcaattggt aaaaggccaa tggatcgtgt cttgagacca ttgaaactta
     2941 tggatgcgaa tattgaaggt attgaagata attatacacc attaattatt aagccatctg
     3001 tcataaaagg tataaattat caaatggaag ttgcaagtgc acaagtaaaa agtgccattt
     3061 tatttgcaag tttgttttct aaggaaccga ccatcattaa agaattagat gtaagtcgaa
     3121 atcatactga gacgatgttc aaacatttta atattccaat tgaagcagaa gggttatcaa
     3181 ttaatacaac ccctgaagca attcgataca ttaaacctgc agattttcat gttcctggcg
     3241 atatttcatc tgcagcgttc tttattgttg cagcacttat cacaccagga agtgatgtaa
     3301 caattcataa tgttggaatc aatccaacac gttcaggtat tattgatatt gttgaaaaaa
     3361 tgggcggtaa tatccaactt ttcaatcaaa caactggtgc tgaacctact gcttctattc
     3421 gtattcaata cacaccaatg cttcaaccaa taacaatcga aggagaatta gttccaaaag
     3481 caattgatga actgcctgta atagcattac tttgtacaca agcagttggc acgagtacaa
     3541 ttaaagatgc cgaggaatta aaagtaaaag aaacaaatag aattgataca acggctgata
     3601 tgttaaactt gttagggttt gaattacaac caactaatga tggattgatt attcatcctt
     3661 cagaatttaa aacaaatgca acagttgata gtttaactga tcatcgaata ggaatgatgc
     3721 ttgcagttgc ttctctactt tcaagcgagc ctgtcaaaat caaacaattt gatgctgtaa
     3781 atgtatcatt tccaggattt ttaccaaaac taaagctttt agaaaatgag ggataatata
     3841 aaatggaaga tatctataaa ttaatagacg atatcaatct acaaaaacta gaaaatttag
     3901 actctcgtgt taatgaagca ataactactg acaacgatga cgcattattt attctaggag
     3961 agacacttta caattttgga ttaatgccac aaggtttgga agtattccgc gtgttatatc
     4021 acaaatatcc agacgaaagt gaattgctga tttattttat tgaaggttta atgtctgaaa
     4081 atcaaactga cgaagcgtta gaatatttaa gttatgttga accatcacct gaaaagttga
     4141 tgttagaagc agatttatat caacaaatta atatgatgga agttgctatt gataaattac
     4201 aagaagcact tgaactagag ccaaatgatc caataatcca ttttgcattg gctgaaatgt
     4261 tatattatga tggtcaatat ttacgtgcta cctctgaata cgaaaccgtt ttagaaactg
     4321 gtgaatatca agttaatggt gtaaacttat tctctcgtat ggcagattgt agtttacaaa
     4381 gtggtaacta tagtgatgcg attcgcttat acgatgaaat taatgaagat gaaatgactt
     4441 cagaagatta tctcaaaaaa gccatttctt acgataaaaa tgacatcact caagaagcaa
     4501 ttaaaataat gactacatta ctttctaaag atcctgatta tattcaaggc tacttgtatt
     4561 tacaatcatt atatgaaaat gaaaaaaatt atccagatgc cattgaaacg ggtaaagaag
     4621 gattacgact aagtcaattt tataaagagt taatgtatac aactggatgt ttggaaatag
     4681 aacacggcga tgctaatgaa ggtgtattat tgttaaaaca agcattagag gttgataatg
     4741 cttaccaaga acctttattg attttaagcg atttatatcg taatgaagaa gattatgaag
     4801 caatcattga attattaaca tatgtagatg aagaagattt ggatcctaca ttcatgtggc
     4861 atttagcgtt cgcctatggt caagaagagc gagataaaga agctcaacat ttctttgaat
     4921 tagcatatcc aacgatgaaa acaaatattg attttatgag tgattattat ttctatttaa
     4981 ttgaaattgg tcaaaaggaa caagcaatta ctattttaaa tcaattgttg gaactagagc
     5041 caagtaacga aaattggcat gacgaattat caagattgca atcttaaaat ggggtatttg
     5101 atgtcagaga cattcaatca aattaaagaa agttttattg aatatttact ttttcaatat
     5161 cgatttaagt cgcgtattgc agtttgggtg cttaactata taaaagtgaa tgaggcgaaa
     5221 ttggcaaaca ttcattttgt agatacaaaa attaatcatc atgaaacatt agaaattgca
     5281 gaagtgggaa gtcacgcttc tgcaattcaa tttactaagc gtaatataaa gttaatgaat
     5341 acaaatgaaa tatttgatta tatcgctaat cataactgtg cttttgatat tcaaattcac
     5401 ttcgcaaacg tgtcaaagcg tgagcaaagg ctagatgatt taatagttgc tcaactaacg
     5461 gaatcgccca gttatcaaac ttatcttcat gacttaaata gtatggctat agatcggcat
     5521 aaacatgcgt tattaataga ttacttgtta cacaatattg atttaagttt acagatgaat
     5581 gaaaagcaac gtttttatca actaacgcaa attttaaata cactcaagct agtaaataaa
     5641 cataatcaat ttgaggactt agcagatgac aattaaagca ttttggcaat atacacttta
     5701 tcaacgttct tggttaatga tgctactcat ttgtaatata ttagggatga tttatggtta
     5761 tatatggtat ggtgaacaat taagtcatac gccctggcat tttaagattt ttgttccaga
     5821 tagcccaact gcaatattat acttagttat ttctatctca ttgatattaa ttcaaaaaca
     5881 aaatagtatt atcgatgcac tggcatttgt aacattattc aaatatggga tttgggcagt
     5941 tataatgaat atattattta ttattgaaca aggtgatatt acagtaaatg gtttggtatt
     6001 aatgttttcc cacagtataa tggctgttca ggcaatttac ttctatccta ggtttaaacg
     6061 ttctatgatt ggaatcagtg tagcgatgat ttgggtgttt ttgaatgatt atattgacta
     6121 ttttcacctt caatttccat attatgattt tataactaca catgtgtggc aaattggtgt
     6181 attatcatgt tgtctaagtg tttttggact tttgctttat attgaattaa acaaattatt
     6241 aaagtgtaaa tgatttgata aaaacggcaa atgacagtaa aatatttata aaggagagtg
     6301 agtttattgt cttcaat
//
LOCUS       DAMQYT010000147         6169 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMQYT010000147 DAMQYT010000000
VERSION     DAMQYT010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.149"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(192..785)
                     /locus_tag="PGA33_002089"
     CDS             complement(192..785)
                     /locus_tag="PGA33_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDG6473890.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            849..1232
                     /locus_tag="PGA33_002090"
     CDS             849..1232
                     /locus_tag="PGA33_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HDG6473891.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHDRKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            1363..3768
                     /gene="mnhA1"
                     /locus_tag="PGA33_002091"
     CDS             1363..3768
                     /gene="mnhA1"
                     /locus_tag="PGA33_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HDG6473892.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            3761..4189
                     /gene="mnhB1"
                     /locus_tag="PGA33_002092"
     CDS             3761..4189
                     /gene="mnhB1"
                     /locus_tag="PGA33_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HDG6473893.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            4189..4530
                     /gene="mnhC1"
                     /locus_tag="PGA33_002093"
     CDS             4189..4530
                     /gene="mnhC1"
                     /locus_tag="PGA33_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HDG6473894.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            4523..6019
                     /gene="mnhD1"
                     /locus_tag="PGA33_002094"
     CDS             4523..6019
                     /gene="mnhD1"
                     /locus_tag="PGA33_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HDG6473895.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVYFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            6021..>6169
                     /gene="mnhE1"
                     /locus_tag="PGA33_002095"
     CDS             6021..>6169
                     /gene="mnhE1"
                     /locus_tag="PGA33_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HDG6473896.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRF"
BASE COUNT         1873 a         1018 c         1033 g         2245 t
ORIGIN      
        1 taaaaacata aaaagcaacc tcaactatta tctttaaaat acacatctat ttaaaaactg
       61 tgctttcttg ataaatatat tgagattgct catcatatta gtttatttat tttcgttgga
      121 atatatacta atccattcat aaattagctt tatccgagtt taaaatgttg gtagttaatt
      181 atgtttagat attattcttc aacatcaata gattcaatta caacatcatg aagtggttta
      241 tcttgtggtc ccacttttgt atttgcaata tcttctaatg tagtttcacc atcaatgatt
      301 tgaccgaata ctgtatgttt ttgatctaac catggtgtac cacccttttc gccatatgca
      361 tcaacgattg gttgaggcca gccaccatct gcaagttgac ttaacatatt ttgaggtact
      421 tctttcattt gaacaatgaa aaattgtgaa ccattagtat taggtcctga gttagccatt
      481 gataatgcgc catataagtt aaatgcattt aatgaaaatt catcttcaaa agcaccgcca
      541 taaatacttt cgccacccat accagtagct gttggatcgc caccttgaat catgaagtca
      601 ttaatgacac ggtggaatgt gattccatca taataaccat tttttgcatg tgtcacaaaa
      661 ttttcaactg tttttggtgc aatatttgga aataatttga atgtcatgtc acctttattt
      721 gtgtgcataa ccactttgat ttcaccttgt tgtacttctt tgtttaactg tggatagtta
      781 gccatttata ttctccattc atgttatgat acatatatat taacatactt ttaagggagg
      841 aaaatagcat gaagctatat cgattttcac acaaaacagg tagctatggt gtcacgattg
      901 ttgaagaaaa tggagacgat gtccttgtta aagttgaaca agttatcaaa cacccgaaac
      961 aaggtgattt acataaccct actgaaactg aaggtgtatt tttccatgac cgcaaagcat
     1021 taagccattt tgaaaaacgc tatacgaaac gttcacaatt acgcgacttc aatgttgatg
     1081 tattgcgcta tgaagattca cttcaacaag caattacaga tttagaaact aaacttaaag
     1141 ctcagcaaac aaaacatgct gaaatgtctt tagctagttt agcacgctta aaagaagatt
     1201 attcacttca atacaaagaa aattttaaat aaaaaatgac gtgtttcttc ttatttaaat
     1261 acttctgaaa ataaagcaca acaaggagtg tgtacgtttt tcagaactat atcattcatg
     1321 tataatgaat aacataaata ttttttaaag gaggacaaca tagtgagttt gttacatatt
     1381 gcggtgatat taccgttaat attcgcactc attattccta tcctgtatag attttttaaa
     1441 cgtatacatt taggatggtt cgtattacct gtccctatcg tcatatttat ttatatgctt
     1501 acattaatta aaacgacgat gtcaggtaat actgtaatga aaaccttaaa ttggatgcct
     1561 cattttggta tgaactttga tttatatctt gatggcttag gtttactctt tagtttgctt
     1621 atttcaggaa tcggcagttt agtagtatta tattcaatcg gctacttaag taagtcagaa
     1681 caactaggga atttttattg ttatttatta ctatttatgg gtgcaatgtt aggtgtcgta
     1741 ctttcagata atgttattat tctatattta ttctgggagc taacgtcatt ttctagtttt
     1801 ttacttattt cattctggcg cgaacgacaa gcatctatat atggtgctca aaaatcgctc
     1861 ataatcacag tatttggtgg tctatcatta ttaggtggta tcatcttact cgctatacca
     1921 acacaatcat ttagcattca gtacatgatt caacatgctt ctgaaattca aaatagccca
     1981 ttcttcattt ttgcgatgat tttaatcatg attggtgctt ttacaaaatc tgctcagttt
     2041 ccattttata tttggctacc agatgcaatg gaagcaccta caccagtcag tgcttatctt
     2101 cattcagcaa caatggttaa agcagggtta tatttaatcg ctcgaatgac accaatcttc
     2161 gcagcatcgc aaggttgggt atggactgtc acactcgttg gtttaattac attattctgg
     2221 gcttcgctaa acgcaacaaa acaacaagac ttgaaaggta tactggcatt ttcaacagta
     2281 tcccaattag gtatgattat ggcgatgctt ggaatcggtg ccattagtta tcactatcaa
     2341 ggcgatgata gcaaaatata tgctgctgca tttacggctg caatattcca tttaatcaac
     2401 catgcaacat ttaaaggtgc cctatttatg attacaggtg cagtggatca ttctactggt
     2461 acacgtgatg ttaagaaatt aggtggctta ctaacaatta tgcctatttc atttacaatc
     2521 actgttatta cagcattaag tatggctggt gtaccaccgt tcaatggttt cttatcaaaa
     2581 gaatcatttt tagaaacaac atttacagca agccaagcaa atttattcag tgttgataca
     2641 ttaggctacc tattcccaat cattggtata gtgggcagtg tcttcacatt tgtatattca
     2701 attaaattta taatgcatat atttttcgga caatataaac ctgaacaatt acctaaaaaa
     2761 gcacacgaag tatcaatatt aatgctttta tcaccagcta ttttagctac attagtaatt
     2821 gtatttggtt tattccctgg cattttaact aattcgatta ttgaaccagc tacatcttcg
     2881 attaatcata cggtaattga tgatgttgaa ttccatatgt tccatggatt aacgcctgcc
     2941 tttttatcaa cattagtgat ttatatttta ggtatcttac taattgtgac atttagttat
     3001 tgggtgaaat tgttgcaacg acaacctggt aaactaacat tcaattattg gtacaacaga
     3061 agtgcaaatg ttattccaaa ctactctgaa aagatgacga atagctatgt taccgattat
     3121 tcacgtaata atttagttat catttttggt gcattaattt tattaacatt tgtcactata
     3181 ttctcagtac ctttcaacat taattttaaa gacgttagtc cgattagaat tttcgaagtt
     3241 tgtatcgtaa ttttattatt aagtgctgcc tttttaattc ttttcgctaa atcaagatta
     3301 tttagcatca ttatgttaag tgcagttgga tatgctgtgt ctgtattgtt tatattcttt
     3361 aaagcacccg acttagcatt gacacagttt gtcgttgaat ctatttcaac agcattgttc
     3421 ttattatgct tctaccactt accaaattta aatcgttata atgaaaagcg ctcattccag
     3481 cttactaatg ctttaattgc tggtggtgtt ggtttgtccg tcattattat cggtttaatt
     3541 gcatacggaa atagacattt tgaatcgata tctaagtttt atcaagagca cgtttatgat
     3601 ttagctcacg gtaaaaacat ggtaaacgtc atacttgttg atttccgtgg tatggatact
     3661 ttattcgagt catctgtgct tggtatcgca ggattagctg tatatacaat gattaaatta
     3721 cgtaaaaagc gtcaaacgca aggtaacgag gtgaaaaatc atgaatagac aacaaaatga
     3781 tctaattttg caatttgctg cagttattat ctttttcatg gtaatggtct ttggtttttc
     3841 acttttctta gcaggacatt atacacctgg tggcggtttc gtcggcggat tattattcgc
     3901 aagttcactt gttatcatta cgattgcatt tgatatagaa acaatgcgta aaattttccc
     3961 attagatttt aaaatcttga taggtatcgg attagttttt tgtattgcga cgcctatcgc
     4021 aagttggttt ttaggcaaaa atttctttac gcatgtcaca tttgatatac cgttatttat
     4081 attagaacct gttcatatga caacagcagt attctttgat ttcggtgttt tatgtgcagt
     4141 agttggtact gtcatgacaa ttattatttc gattggagag aacgaatagt ggaaattatt
     4201 atgatttttg ttagtggtat tctcacagca attagtgtct atctcgtttt gtctaaaagt
     4261 ctgatacgaa ttgttatggg aactacacta ttaacacatg cagcaaattt atttttaata
     4321 actatgggcg gacttaaaca tggtactgtt ccaatttatg aagcgaacgt aaaaagctat
     4381 gttgatccta tcccgcaagc acttatttta acagcaatcg ttatcgcctt tgcgacaaca
     4441 gcctttttct tagtattagc atttagaaca tataaagaat taggcacaga taacgttgag
     4501 agtatgaaag gagttccaga agatgattga aagtaatatg cttgtgttaa cgctagttat
     4561 tccagtaatt acagccatat tacttgtctt tattggaaag cgtcctatta taaagcgtta
     4621 tgtcgcacta ggcggtacat tattaacctt ggtcgcggca attatcaatt tagcaaatgt
     4681 tgttaaacat ggaccaatac gcgtagagct aggttcatgg aaagcaccat acagtattgt
     4741 ctttgtatta gacatattca gtgccctact aataattact agtattatta ttacagctat
     4801 tgtgatttta tattcatacc aaacaattgg tattgaacgt gaaagatatt attattattt
     4861 ttcagtacta tttatgttaa taggtatcat tggtgcattc acaactggtg acattttcaa
     4921 cctattcgta tatttcgaag tatttttaat gtcatcttat ttcttattag taattggttc
     4981 tacaaaaatt caactacaag aaacaataaa atatgttctt gttaacgttg tatcgtcatc
     5041 cttcttcgta atgggtgtag cgattttata ttcagtagta ggaacattga atctagcaga
     5101 tataagcaat aaattagcta acctttcagc gcatgacagt ggcttagtca atatcgtttt
     5161 catactattt atctttgttt ttgctacaaa agctggcgta ttcccaatgt ttgtttggtt
     5221 acctagtgca tactatgcac caccaattcc aatcattgca ttctttggtg ctttgttaac
     5281 aaaagttggt gtttatgcaa tcgctagaac attgagccta ttctttagtg ataatgtaag
     5341 cttttcacat tatgtcattt tattcttagc actattaacg atcatctttg gttgtgtcgg
     5401 tgctgttgca tatgccaaca ttaaaaaaat catcctatac aatgtcatga ttgctgtagg
     5461 tgtgatctta gtcggcgttg caatgatgac agagtcaggt atgattgggg ctatttacta
     5521 tacattgcac gacatgttag taaaacttgc attattcttg cttatcggaa ttatgattaa
     5581 aattacaggc actgctgatt taagacaatt tggtggctta attaaacgat atcctgtact
     5641 tggttggtca ttctttatcg cagcattaag tttagctggc attcccccac tcagtggctt
     5701 ttatggaaaa ttttttattg tccaatcaac atttgaaaga ggattttatt taagtggtgt
     5761 catcgtctta ttatcaagtt tagtcgtatt atattctgtc attcgcatat tcctacaagg
     5821 ctttttcggt caaccaaaag gatatgatct taataacaaa gtcgacgtca aatatttaac
     5881 tacgattgca atcgttgcag ttgtgataac tgttttatat ggattgtcag cagattactt
     5941 atatccaatg gtaaaagctg gtgcagaaac attctacaat ccatcaacct acgttaaagc
     6001 tgtattagga ggtaaataac atggctgtac aattagtatt aaactttatt atcgcagtgt
     6061 tttggttgtt tgtgacaaat agttacacaa caaataactt tgtgttaggt ttcatctttg
     6121 gcctagttct tgtttacctc ttacacagag tgttaccagg tagatttta
//
LOCUS       DAMQYT010000148         6164 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMQYT010000148 DAMQYT010000000
VERSION     DAMQYT010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.150"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..220
                     /locus_tag="PGA33_002096"
     CDS             <1..220
                     /locus_tag="PGA33_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473897.1"
                     /translation="YYALSRSLPLFLLAIFSLVIHSAIFLITISIAMILVQFLDAIVG
                     YKSEDVFKTYGPLATSVVNLILLIVFLF"
     gene            complement(226..363)
                     /locus_tag="PGA33_002097"
     CDS             complement(226..363)
                     /locus_tag="PGA33_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDG6473898.1"
                     /translation="MMVDKATTQIQHDCGIRVLVFIKLIESYIKYSLDNSILVRLRFV
                     V"
     gene            complement(550..1947)
                     /gene="sufB"
                     /locus_tag="PGA33_002098"
     CDS             complement(550..1947)
                     /gene="sufB"
                     /locus_tag="PGA33_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDG6473899.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            complement(2098..2562)
                     /locus_tag="PGA33_002099"
     CDS             complement(2098..2562)
                     /locus_tag="PGA33_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDG6473900.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            complement(2552..3802)
                     /locus_tag="PGA33_002100"
     CDS             complement(2552..3802)
                     /locus_tag="PGA33_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDG6473901.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            complement(3908..5215)
                     /gene="sufD"
                     /locus_tag="PGA33_002101"
     CDS             complement(3908..5215)
                     /gene="sufD"
                     /locus_tag="PGA33_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDG6473902.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            complement(5313..6074)
                     /gene="sufC"
                     /locus_tag="PGA33_002102"
     CDS             complement(5313..6074)
                     /gene="sufC"
                     /locus_tag="PGA33_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDG6473903.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
BASE COUNT         1870 a         1204 c          876 g         2214 t
ORIGIN      
        1 atattatgca cttagtcgaa gcttaccttt atttttatta gctatttttt ctttagtcat
       61 tcatagtgct atatttttga taactatatc cattgcaatg atcttagttc aatttttaga
      121 tgcgattgtt ggttataaaa gtgaagatgt ctttaaaact tatggtccat tagcaacatc
      181 tgtagtgaac ttaatattat taatagtttt cttattttaa ctcacttata caacgaatct
      241 taatcgaact aatatcgaat tatctaaaga atatttgatg tagctttcaa ttaatttaat
      301 aaagaccagc actctaatgc cacaatcatg ttgtatttgt gttgtcgctt tatccaccat
      361 caatgattat tttttacacc aatcaaaaaa tcgaactgat ataaataagt acaaagctta
      421 tctatcaatc cgatttagtt ataaaacaaa aaaagccaca gtaatgtggc tttttgttat
      481 attcagtatc aaaatggtat caatagccat tttcggaagt caagaatggc ttaacaacgc
      541 ggtttaaagc tatccaatac taccttccat ttcgaacttg attaaacggt tcatttcgac
      601 cgcgtattcc attggaagtt cttttgtaaa tggttcgatg aatcccataa caatcatttc
      661 tgtcgcttct tcttcagaaa taccacgact cattagatag aataattgtt cttcagaaac
      721 ttttgaaacc ttggcttcat gttctaatga tatttgatcg ttgaatactt cgttatatgg
      781 aattgtatct gatgttgatt cgttatctaa gattaatgta tcacattcaa tatttgaacg
      841 agcacctttt gctttacgtc caaaatgaac aataccgcga taaataactt taccaccatt
      901 tttagaaata gatttagaaa caattgtaga agatgtatta ggtgctttat gaatcatttt
      961 agcaccggca tcttgaactt gtcctttacc agcaaatgca atagataatg tactaccttt
     1021 tgcaccttca cctaaaagaa cacagtttgg atatttcatc gttaacttag aacctaagtt
     1081 accatctacc cattccatat ttccgttttc ataaacaaaa gtacgttttg taactaaatt
     1141 gtatacattg ttcgcccagt tttgaatcgt agtataacga acgtgcgcat ctttatgcac
     1201 aatgatttcc acaacagcag agtgtaaaga actagttgta taaactggtg cagtacaacc
     1261 ttctacgtaa tgtacagaag caccttcatc agcaatgatt aatgtacgtt caaattgacc
     1321 catgttctca gagttaatac ggaaataagc ttgtagtggc gtatctagtt tgatattttt
     1381 aggtacataa atgaacgaac cacctgacca tactgctgag tttaacgccg caaatttgtt
     1441 atctgctgca ggtactacag aagcaaagta ttttttgaat aattcttcat tttcttgtaa
     1501 agcactatct gtatctttaa agataatacc tttttcttca agttcttttt ccatattatg
     1561 gtaaacaact tcagattcat attgagcaga aacaccagct aaatattttt gttcagcttc
     1621 aggaattcct aatttatcga aagttctttt aatttcttct ggcacttcat cccatgaacg
     1681 ttcagcttgt tctgaaggct ttacatagta agtaatgtca tcgaaattca attctgataa
     1741 gtcgccaccc cattgaggca ttggcatttt ataaaacaat tttaatgatt taagacggaa
     1801 atctaacatc cattccggct catttttcat gttagaaatt tctctaacga tattctcagt
     1861 taaaccacgt tctgatctga aaatggacac atcatcgtcg tggaatccat atttataatc
     1921 cccaacatca ggtgcttttt tagccatttc aatcactcct taaaaatata tttacaacgg
     1981 cgtgcgtaag tttttaattc atgatgtaaa ccatattata acaatgacat gacatcttat
     2041 aaaaattttt atacttttat atgtctaata tcaaaattat ctatgattaa cagcattcta
     2101 ttcttcttca gtcgtacctt ctgctttacc ttctttagca acagtacctt tttccaatgc
     2161 tttccaagct aatgtggcac atttaatacg agctgggaat tgagatacac cttgcaatgc
     2221 ttcaatatct cccatttctt ctgtaatcac atagtcttca ccaagcatca ttttcgtaaa
     2281 ttcttggctc atttgcattg cttctccaag tgaatgacct ttaacagctt gtgtcatcat
     2341 cgatgcactt gccattgaaa tcgaacaacc ttcaccttca aacttagcat cttttataat
     2401 gccgtcttct atatcaaatg ttagtcgtat acggtcaccg catgtcgggt tattcatatc
     2461 tactgtcata gacccgttat ctaatacacc tttatttcta ggatttttat aatgatccat
     2521 aatgacagat ctatataatt gatctagatt attaaaattc ataagagaaa aactccttcg
     2581 tttgtttcaa ggcatttatt aactgatcaa cgtcttcttt cgtgttgtat atataaaaac
     2641 tcgctctagc tgttgaagac acatttaacc atttcattaa cggttgcgca caatgatgcc
     2701 cagctctaac cgctacacct tctgtatcta cggctgtagc aacatcgtgt ggatgtacat
     2761 cttgtaaatt aaacgttatt acacctgcac gacgatcctt tggcgggcca taaatttcaa
     2821 ttccttcaat tgcagacatt tgctcataag catatatcgt taattcttgt tcatatttat
     2881 gaattgcatc aaaacctatg cgttctaaat agcgaatagc ttctgcaagc ccaattgctt
     2941 gagcaattaa tggagtaccc gcctcaaatt tagtaggtaa atcagcccat gttgcatcat
     3001 acttacttac aaaatcaatc atgtcgccac cgaactcaat cggttccatt ttttgtagta
     3061 actcacgttt accaaataat acgccaatac ctgttggtcc aagcatttta tgaccactaa
     3121 aactataaaa atcagcattc atttcttgca tatcaagttt catatgtggt gctgcttgcg
     3181 ccccatcaac actgataatt gcaccatgtt gatgagctat ttctgcaatg gttttaacat
     3241 cattaattgt accgagcaca ttagatatat gtgcaatagc aacgatcttt gttttatcat
     3301 taatcgtttg cttaatatcc tcgatgttta attcaccgtc agctgtcatt ggtataaatt
     3361 tcaatgtcgc atttttacgc tttgctaact gttgccaagg aacaatattg gcatgatgtt
     3421 ccatttcagt gacaacaatt tcatcgccct cttcaacatt tgcatcacca tagctatgtg
     3481 ctacaaggtt aatcgacgca gttgttccgc gtgtaaaaat gatttcttca aaatacttcg
     3541 cattaataaa acgacgaacg gtttcacggg cattttcata accatcagtt gccaatgatc
     3601 ctaatgtatg aacaccacga tgaacgtttg aattataacg cttgtagtaa tcttctaaaa
     3661 catttaacac ttgcacaggc gtttgacttg tcgctgttga atcaagatat gctaaacgtt
     3721 tgccattgac tttttgatct aatatcggaa aatccttgat tacttcatta acgtcaaatg
     3781 agtgttcggc cacttcattc acgacctttc ttaaataaaa atcctaatca tttaaatact
     3841 gacgttgtat tagtcttata ccaatatcga cagtctatat ctattacaaa cttttatttt
     3901 caaaatatta tttagaaact ttgcgttcaa ttacttctct caattgacgt ttaacgtctt
     3961 cgataggtaa ttcacgtact actggatcta agaaaccatg tataacaaga cgttccgctt
     4021 ctctttgaga aataccacga ctcattaaat agtaaagttg atctggatca acacgaccta
     4081 ctgatgcagc atgaccagct tgtacatcat cttcatcaat taataaaata ggattcgcgt
     4141 caccacgagc atgttcagat aacattaata cacgtgattc ctgattagca attgatttag
     4201 ttccaccatg cttaatgtag ccgataccat taaatacaga cgatgcatgt tctttcataa
     4261 caccatgttt aaggatataa ccatctgttt ctttaccata ttgtacgatt ttagatgtta
     4321 gattaatttt ttgttcgcct gtacctacaa ctactgattt aagtgaactt gttgaacgat
     4381 caccaaataa atttgttgta ttatcaataa tttggctacc ctcattcatt aaacctagtg
     4441 cccaattaat tgaggcatcc gcttcagtaa taccacgtcg aatgatatga cctgtaaagc
     4501 ctttatccat atagtccact gagccatatg tgatatttga atttgcacca gcaatcactt
     4561 cagaaataat atttaattga tttccttcac cagatgcatt tgataagtaa ttttcaacat
     4621 atgtgacttc ggcgctttct tcagtaacga tgataacatg gttataaaag cttgcatttt
     4681 cgtcgtcgtg caacacaacg tattgtactg gatgttctac aactacattt ttaggaacat
     4741 aaacaaatac gccaccatta actaatgccg tgtgtagcgc tgtgatacga tgttcatcta
     4801 ctgttactgc atctttcata aagtactttt gtactaaatc actatggttc ataagagcgt
     4861 ctgctaaacc ttcaacgata acgccatctt tcgatgcatt atcatcaact tgtgtgtacg
     4921 caatcgtatt attatgttga attactaagt ttttagaatg atctacgtca ataatttctc
     4981 ttactgactc aggtaattgt gataaagatt gataaacatc accttttaca tcgtgttgtt
     5041 taaaagaatc aaaatcccat tttcttaatt ttgttttatc aggttttggc atttctaaag
     5101 tttctgttaa tttcaaagct tttttacgta attctgtcat ccaagaaggt tcattgtggg
     5161 ctttagaata atcaacaagt tgttcttcag aaatgttcaa aatatcagtt gtcataactt
     5221 tttcctccta cttgatgaaa tttactattt cgtataaatg ttcattttca tcatatatag
     5281 atgaacatct catggatact gtattaataa gattattcag ctgaaccgaa ctcttcttta
     5341 acccattcat atccttcttc ttcaagacgt tttgctaatt ctggaccacc agatttaacg
     5401 actttaccag catacattac atgtacttta tcaggagtaa tgtaatttaa taatcgttga
     5461 tagtgtgtaa tcattaatgc accaaagttt tccccacgca tttggttaat acctttagat
     5521 acaactttta atgcatcgat gtctaaccct gaatcgattt catctaagat tgcaaactta
     5581 ggttctaaca tcattaattg taagatttcg ttacgtttct tctctccacc tgagaaacct
     5641 tcatttaaat aacgttgtgc catgtcttta tctatgtcta gaaaatccat gtttttatct
     5701 aatttcttaa taaattgcat taagttgatt tcttgtcctt cttcacgttt cgcattgatt
     5761 gctgaacgca tgaaatcagc atttgtaaca cctgtaattt ctgatggata ttgcattgcc
     5821 aagaataatc ctgcttttgc tctttcatca acttctaatt ctaaaatatt tacaccgtct
     5881 aaaagtactt ctcctttagt tacttcatag cttgggtgtc ccataattgc agatgataaa
     5941 gttgatttac ctgtcccgtt tggtcccata atcgcatgta tttcatcagt gttaattgtc
     6001 aagttaacac cttttaagat ttctttatcc tcaatagaca catgtaggtc tttgatttct
     6061 aatgttgatg ccattttata ttccctccgt aaaatataaa gttttcttaa ctagtttata
     6121 ataattttaa tttgtagtca aaaagacttt gtaataatgc gttc
//
LOCUS       DAMQYT010000149         6153 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMQYT010000149 DAMQYT010000000
VERSION     DAMQYT010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.151"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..195)
                     /locus_tag="PGA33_002103"
     CDS             complement(<1..195)
                     /locus_tag="PGA33_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDG6473904.1"
                     /translation="MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGM
                     CTTIEDEFNIHQFRDILSPEK"
     gene            305..1174
                     /locus_tag="PGA33_002104"
     CDS             305..1174
                     /locus_tag="PGA33_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDG6473905.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYEKAQFLSIND
                     VHTSIKLIEMNQGISFLPLYITTNSDYNISVINTKILQAPISFTYIYSKKESPEILVF
                     IKSFKKYIANEQL"
     gene            complement(1233..3842)
                     /gene="clpB"
                     /locus_tag="PGA33_002105"
     CDS             complement(1233..3842)
                     /gene="clpB"
                     /locus_tag="PGA33_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDG6473906.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
     gene            complement(4045..5859)
                     /locus_tag="PGA33_002106"
     CDS             complement(4045..5859)
                     /locus_tag="PGA33_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDG6473907.1"
                     /translation="MNKTKGFTKYKKMRYIPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WYKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
BASE COUNT         2011 a         1106 c          770 g         2266 t
ORIGIN      
        1 tttttcagga cttaaaatgt ctctgaattg atgaatatta aattcatcct cgatagttgt
       61 acacatgcct acttcaacag aatttatatt taacttgtta atttgtttca atacttcttt
      121 ttttataatt agactttttc gaacattact ttgttgcata ccatccctta ttgtattatc
      181 ttgaattcga atcatattac ttcaccccct cgataacctc tgatataata ttaatattat
      241 ttttatgcaa taaaaattaa atattatcta atgaattatt aacttttttg aaagaggtaa
      301 gttcatgaat ttagattggt actatacatt tgtcatttta gcgaagacgt taaactatcg
      361 attagctagt gaagaaatta atctcactat accttctata cataagcaaa ttaaaaattt
      421 agaacagcat ctcaatgtga aactatttga aacttataaa aatcaaatta tcttgactga
      481 agatggtcat acatttcttc caattgcgca aagttttatt gaacaatatg aaagtggcat
      541 caaacatatc caacttaaaa aaacgatgtt tcaatcgaaa ttaaatgttg tcgtgtcttc
      601 atatatcgcg acattcatta tgcccaaatt tttaaaatct ttctttaacg agcatccttt
      661 tatcgatgtt tctcttcatg tcaaaaatga aaatattgaa aaagatatta ataatcatac
      721 gtatgacatc gggattagca gaaatcaacc taaactaaga gaagttcatt ctgaaaaagt
      781 atgcgaaggt aaaattgtat tgattgctcc caataaagag aataatcatc ttctaactga
      841 ggcatcttta tttgaaaaat acaaaataat aagtgataac catccagaat attggtcatc
      901 acttaaaaat aatattttaa atatttatga aaaagcacaa tttctatcta tcaatgatgt
      961 gcatacttca attaaattaa tcgaaatgaa tcaaggtata tcattcctgc ctctttatat
     1021 aacaacaaat agtgattaca atatttcggt tattaacacg aaaattttac aagcacctat
     1081 ttcatttaca tatatataca gcaaaaaaga aagcccagaa atattggtgt ttattaaatc
     1141 atttaaaaag tatattgcca atgaacaatt ataataaatt tcaaatctaa aaaaccaaga
     1201 atgcgattaa tcatcacatt cttggttcaa ttttattcat gaattttttc aacattaaac
     1261 gttaagttat tgtctgaatt taaattaact ttaatcgttg taccttctgg gaatccctct
     1321 ttaatcatca tacgtgctaa tggtgtttca atttggcgtt gtacaaaacg ttttaatggt
     1381 cttgcaccgt attgaggttc ataagcttct tgacctagcc aagctttagc atcatcagaa
     1441 acttcaattg agattcgttg ttctaataat cttatattta attgcgttaa gattttatct
     1501 acaatcatac tcatgtcatc aatagataat ggtttaaata atacgatatc atccatacga
     1561 ttcaaaattt ctggtttgaa atatgcattt aaacttgtca taacagcttt ttctgttgat
     1621 tctgtaattt caccagtctc ttttacgttt tctaataaaa cttgagatcc aatattactt
     1681 gtcataataa taatagtatt tttaaaatca acgctacgtc ctttagaatc agttaaacgg
     1741 ccttcatcta aaatttgcaa taatacatta aagacgtcag tatgcgcttt ttcaacctca
     1801 tctaataaaa taactgagta tggattacga cgaaccgctt cagttaattg acccccttca
     1861 tcatgtccaa tatatcctgg aggtgcccct atcaatcttg atactgcatg tttttccata
     1921 tattcactca tatcaatacg aatcatatgt ttttcagaat caaataatga tgcagctaat
     1981 gatttagcta attcagtttt acctactcca gttggaccta ggaataagaa actaccaata
     2041 ggtctgtttg gatctttaat acctgctctt gctctaacta ctgcatctga aaccaggtca
     2101 accgctttat cttgacctac aacacgttta tgcaagatgt cacttaagtg aagtaatttt
     2161 tcacgttctg tttcaactaa ttttgaaact ggtatgcctg tccattggct gacaatatcg
     2221 ccaatttctt cgtctgttac aacttcacga atcattcgat ctgtatcttc accttgctca
     2281 tcttggaaat tatcctctaa ttctctaagt tctttttcca attgaggaat tgttccatat
     2341 tgtagttcag cagctttttc taaattgtta tttgtttgtg catcttccaa cgcttgtcta
     2401 ctttcatcta gttgcgcacg tttttcttgt aaatttgcta ttttttcttt ttctgattct
     2461 acacgagatt gaagtgctgc ttgtttctct ttttcattgg caagctcttc ttgtagttct
     2521 tgtaatctct gtttgctcgc attgtcagat tcatttttaa gtgcgctttc ttcaatttct
     2581 aattgcatga cacgtctatt aacttgatcc aattcagttg gatttgatcc catttccgta
     2641 cgaattgttg cacatgcttg gtcaactaaa tcaatcgctt tatctggtaa aaaacgatca
     2701 gtgatgtaac gatcagacaa ttcagcggca gcaactaagg ctctatcttg aatacgcaca
     2761 ccatgataca cttcatatcg ttcttttaaa ccacgtaaaa ttgaaattgt atcttcaaca
     2821 tcaggctcac taactgctac tttttggaaa cgacgctcta atgccgaatc tttttcaata
     2881 tattctcgat attcatttaa agttgttgca ccaatacaat gtaactctcc tcgtgctaac
     2941 attggtttta gcatgttgcc tgcatccatg gcaccatctg ttttaccagc acctacaagc
     3001 atatggattt catcaataaa taatataatt ctaccatcag actctttaac ttcttttagg
     3061 actgctttta atctctcttc aaattcacca cgatatttag cgcccgctac taatgcgctt
     3121 aaatctaact caaaaacagt tttatctaat aatgattctg gcacatcttt cttaactata
     3181 cgttgcgcta atccttcaac aattgcagtt ttaccaacac ctggttcacc aatgagcaca
     3241 gggttgtttt tagttttacg acttaaaata cgaatcgtat ttcgaatttc ttcatctctt
     3301 cctataacag gatccatttt accttgtcta acttcttcta ctaagtcgcg gccatattta
     3361 gctaatgctt cgtaattaac ttctggattt tgtgatgtca cgtgatttcc ccctcttact
     3421 tttttaataa tttctttgat aacttctact ttattattta tataatgttt tgttgtttga
     3481 tcaatgtcca ttgccgaacg taaaatatgc tccattgaaa tatattcatc ttcatattct
     3541 ttcatgtatg attcagcctt agttatcaat tggtttgctt gttggctaat atattgacca
     3601 tattgtatat tgtcaccttc tacagatgca tacgtgttta gtttgtcttc ataagctttg
     3661 tttaattgat ctacctcaat atttgctcgt tctaaaatac ttttatataa gctttcactt
     3721 tcatttaagg cagcgcttaa aattgcctca atttctatat tttgtaattt atgttgctga
     3781 ctcagttcaa ctgcttgttg taaagcactt tgaacagcat atgtcatttt atttatatcc
     3841 atataatttc acctctaatt tgtagagttg gtcaagttgc acttttgact ttgactttct
     3901 ttgaccttaa ttatattata gtcccaatat aatcatttat caactctttt acacttaaat
     3961 ttcctaatat ttgtaaatat ttttttgaat aaaaaaatct agctaatgtt acgttacaca
     4021 ttaactagac ttattaaaca tatgttattt ctttttaagt gcttttaata tttcatcttt
     4081 taaagctagg actcctttgt actctaaatg tacaccgtct ggtgcaaaat attcactatg
     4141 tccttgtgat cgcttatacc aatcaattaa tgtgacattg gactttcgtt tcgccgcgtc
     4201 agctaataat cgatttacat ttgcctcata aattcttgga acacgtgtat taactaaata
     4261 aatcttggct tttccaaatt gattaagtaa atcgtcgagc tgtttgacag taaagtcgcc
     4321 atttgtacct aattctaata cgacttgatc agatgatttt ttatattgtg aataattcgc
     4381 tttaactaaa ggtaaggttt gatacaattg acgccctact tttccatcaa ttctagactt
     4441 aggaactgat gacttaaatg actcaccgat atcaaccatt actgaatcac cgattaaaag
     4501 aggtttaata ttattatata cgtcggaaga ttcctttttc ttctcactta ctaagccatc
     4561 aatgtgtatg ttatcaattg gtgctatccg cattaaataa ttatcttcaa ttgtggtatc
     4621 aaagctattc gctttttctc caataatgtc tttaccatat ttattgaatg cacctactaa
     4681 aatcaacata aatggaatta acaaggttac tacaattgcc attcttataa attgtggtat
     4741 ataagaaggt cgccaattta aagctttaat accttctttt ctaaatggag tttctataaa
     4801 gcgatatgat agctctgcaa atataattgt taaacttata tctataaagt acacatatac
     4861 aggtatctgt ccgtctacat agtaactatg tacgaaacta attactgcaa aatgccataa
     4921 atataaacta taagaccttt tcccgataaa tactaacact ggatttgaaa atatcttcgc
     4981 tatccatgta gatggatgaa cgacactagc aataataaat aacgttaata tggatattaa
     5041 atagaaacca ccatcatata tccaattcgt ctcatcatta ataatgaaaa ataataatat
     5101 aagtactata aatgataaac tacctatgct atcaataaca tattttacaa ctttaggtgg
     5161 atcatttttc aatttaaacg gtggccataa aaaagctaaa ataacaccca gtaacaatgt
     5221 ctgtaatcta gtatctgtac caaaatacac tcgtgaatga tccccattaa tactatagat
     5281 aaacatcatt aaccctaaag aaattattga tactccccaa aaaataaatc ctattttgta
     5341 tcgcttttta attgttaaca ataatgtaac caaaataact gggaaaaata tgtaaaactg
     5401 ttcttcaatt gctaaagacc ataaatgctt taatggcata aatgaaaatt gctcaaaata
     5461 attaacatct tttgctatat accaccagtt tgatacataa aatatcgcag caataatatc
     5521 atgtttaacc ctaatgatat tatctgattt taataataag gttgctgtcc ctacaaccat
     5581 taataaaact atgactgctg gtaataaacg ttttaaacga cgtatccaaa agcttttcaa
     5641 tttaatgata cctgtgtcat catactcttt gagtaataag cttgtaatta aataaccaga
     5701 gatcacaaaa aatgtatcca cacctaaaaa gccacctgtc aaccattgct tatttaagtg
     5761 gtaaataata attcctagaa cagcgattgc cctcaaacca tcgagccctg gaatatatct
     5821 cattttctta tactttgtaa aaccctttgt tttgttcatt tttgcattct tcccttttaa
     5881 aactgttctt cttagatgct taattaaatt tagttatgct gtttaaaaga atattgaaat
     5941 gcatatgtat attattgaat tacgacatca tcaaaatcat attgactaaa atactgttaa
     6001 attaaaaaaa ttaccaatga tgattcttac ttcgaaatcc aatttgtaat gcaactcggc
     6061 aacttaaaaa ctatgaagta ttatgtattg taatataact gtaatataaa ttaaatttat
     6121 tataaaaatt ttcaagaaaa tattcaacta gaa
//
LOCUS       DAMQYT010000150         6095 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMQYT010000150 DAMQYT010000000
VERSION     DAMQYT010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.152"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(101..325)
                     /locus_tag="PGA33_002107"
     CDS             complement(101..325)
                     /locus_tag="PGA33_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDG6473908.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(342..545)
                     /locus_tag="PGA33_002108"
     CDS             complement(342..545)
                     /locus_tag="PGA33_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HDG6473909.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYSNIELIKSLSI"
     gene            704..1264
                     /locus_tag="PGA33_002109"
     CDS             704..1264
                     /locus_tag="PGA33_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDG6473910.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            1283..3685
                     /gene="mnhA2"
                     /locus_tag="PGA33_002110"
     CDS             1283..3685
                     /gene="mnhA2"
                     /locus_tag="PGA33_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HDG6473911.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYVYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVLALRAV
                     SGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     SYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            3672..4097
                     /gene="mnhB2"
                     /locus_tag="PGA33_002111"
     CDS             3672..4097
                     /gene="mnhB2"
                     /locus_tag="PGA33_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HDG6473912.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            4094..4438
                     /gene="mnhC2"
                     /locus_tag="PGA33_002112"
     CDS             4094..4438
                     /gene="mnhC2"
                     /locus_tag="PGA33_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HDG6473913.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            4428..5924
                     /gene="mnhD2"
                     /locus_tag="PGA33_002113"
     CDS             4428..5924
                     /gene="mnhD2"
                     /locus_tag="PGA33_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HDG6473914.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            5925..>6095
                     /gene="mnhE2"
                     /locus_tag="PGA33_002114"
     CDS             5925..>6095
                     /gene="mnhE2"
                     /locus_tag="PGA33_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HDG6473915.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWV"
BASE COUNT         1912 a          847 c         1081 g         2255 t
ORIGIN      
        1 aaaaaaacag gacattatta caacaaaatc accctagaca atcatgatga aagtctaggg
       61 tgttagtatt tatactaaat taattgcgct ttctttatat ctatttctta tcttcaaata
      121 aaacagttgt tgttttatca ataatttgag cagaatgtac tttggtgata gtaccactat
      181 ttgactttat aatatttaaa tctactaatg cattcatact atcacgtgca acttgctcgg
      241 tcactacgtt gtttaattgt ggtaattgca gttttacagg cttgtctagt gaagatttaa
      301 aacttaattc aagtgtttta gtcatgaata tgtcctcctg attaaattga taaagatttg
      361 atgagttcga tattgctata tgtttctcca gtaagtcgct caataagttt gctgaatgtt
      421 ttaatttggt cgtttgatgc atcagggtta atgttagcga atcgacgctt aaattctgtt
      481 tgtttgccgt tagcgtctac tttagtaaat gataatacaa tagtgatgtg gtttatttta
      541 ctcatatttt aaaacctcct ttcacactat atatcgaaac aaaataataa aatggctaat
      601 tttattttct atgtttaaaa tctataaaaa aggcaataga tatatgtaac taaaatataa
      661 tatagctatg attaagacac ttaaagcaag gggaggttga gtaatgaata aagtagaagc
      721 gattaaattt aatgatgata ttgttaaaat gtatgaagcg ctcaagataa aatctgaacg
      781 tgactattta ttctttaagt tagctataca tagtggattg aaagtatcag aattattaac
      841 aattacagtc tctcaagtta agagactaat tgaaaagtgt acgttatcag aaatgtgtaa
      901 agcacatttt cattcgttga ttaaaattag gttaccagaa acattatcga aagaactact
      961 tcaatatata gaggacagga gtctttcgaa tgaagacgtt ctttttcaat cactacgaac
     1021 aaatcaagta ttatctagac agcaagcata tcgaataatt caccaagcat caattgaagc
     1081 tggtatagat aatgtaggac taacgacatt gcgtaagaca tttgcatatc atgcttatca
     1141 aaaaggtata cctataccag tcattcaaaa gtatttaggg catcaatctg ctattgaaac
     1201 actaaatttt atcggtttag aaaatgagtg tgaacatagt atttatattt cattacaatt
     1261 atagaaacaa aggaggctaa taatgagttt ggtttattta ttaattgcta tacttgtgat
     1321 tatggcgatg atacttctaa tgtctaaacg tagagcattg gctaaatatg ccgggtacat
     1381 agcgttggtt gcacctgtaa tttcatctat ctattttttg attcaaatac catcagtagc
     1441 taaactgcaa tatctttcta cctctattcc atggattaag acattagata ttaatttaga
     1501 tttacgttta gatggtttaa gtttaatgtt ttctcttatt atttcactta ttggaattgc
     1561 agtattcttc tatgcaactc aatatttatc ctctcgaaaa gacaatttac caaggtttta
     1621 tttttattta acgttattta tgttcagtat gattggtatt gtattatcag acaatacgat
     1681 attgatgtac attttttggg aattaacgag tgtatcatca tttttattga tttcatattg
     1741 gtataacaat ggtgacagtc aatttggtgc gattcaatca tttatgatta cagtatttgg
     1801 tggattggcg ttattagttg gttttattat gctgtatatc atgacaggaa cgaataacat
     1861 cacagagata ttaggacaag cagatcatat taagaatcat ggattgttta tccctatgat
     1921 ttttatgttt ttattaggtg catttacaaa atcagcacaa tttccatttc atatttggct
     1981 acctagagca atggctgcac ctacacctgt aagtgcttat ttacattcag ccacgatggt
     2041 aaaagctggt atctttttat tacttcgatt tacaccatta ttaggtctta gcaatatgta
     2101 cgtatatatc gttacgtttg ttggtttaat aacaatgtta tttggttcaa ttacagcttt
     2161 aaaacaatgg gatttaaaag gtatcctagc gtactctaca atcagtcaac ttgggatgat
     2221 tatggctatg gtgggtatag gtggcggata tgctcaacac caacaagacg caatagcatc
     2281 tatttatgta tttgtattat ttggtgcgct atttcatcta atgaatcatg ccatctttaa
     2341 atgtgcgctt ttcatgggag taggtatttt agatcatgaa gcaggttcaa gggatatacg
     2401 aattttaagt ggaatgcgtc aactatttcc taaaatgaat ctagtcatga cgatagcggc
     2461 tctatctatg gctggagtac catttttaaa tggattttta agtaaagaaa tgtttttaga
     2521 tgcattaaca caaactggac aattatccca atttagtttg atttcaatga tagctatcgt
     2581 gtttgttggt gttattgcga gtgtttttac attcacatat gcactataca tggtaaaaga
     2641 agtattttgg acaaaatatg attctaaggt ttttactaaa aaaaatatcc acgaaccatg
     2701 gttgtttagt ttaccatctc ttatattaat ggtgctagta cctgtaatct tttttgtacc
     2761 aaatatattt gggaagggga ttatcgttct agcattaaga gctgtatcag gtggtaatca
     2821 tcaaattgat caattggcac cacatgtttc gcaatggcat ggatttaaca taccgcttct
     2881 tttaaccatc atcattattt tattgggtag tgtactagca atcaaagtag attggaaaaa
     2941 agtgttcaca ggtaaaatta gacagatttc agtttcaaaa agctatgaga tggtatatcg
     3001 acattttgaa aagtttgcta cgaagcgatt taaacgtgtt atgcaagatc gtttaaacca
     3061 atacattatt atgaccttag gcatatttat gattatcatt ggatatggtt atattcgaat
     3121 tggacttcct aaagtacatc agttacatgt ttctgaattt ggggcattag aaattatatt
     3181 agcaatcgta actgtcacaa ttggtatttc tttaattttt atacgtcaac gactgacaat
     3241 ggtcatttta aatggagtca tcggatttgt tgtgacctta ttctttatag caatgaaagc
     3301 ccctgatcta gcattgactc agctagtagt tgaaacaata acgacgatac tatttattgt
     3361 cagtttttca agattaccaa acgtgccaag atctaacgct aacaaaaaaa gagaaataat
     3421 taaaatttct gtatcactct tgatggcact tattgttgta tcattaattt ttattacaca
     3481 acaaacagat ggtttatcat caatatcaga cttttattta aaagctgaca aactaacagg
     3541 tggtaaaaat attgtaaatg cgatacttgg tgactttaga gcattagata cattatttga
     3601 aggattagtg ttaattatta ctgggctagg tatttacaca ttattaaatt atcaagatcg
     3661 gaggggacaa gatgaaagag aatgatgtcg tgttaagaac ggtcacgaaa cttgttgtat
     3721 ttattttatt gactttcgga ttctatgtct tcttcgcagg tcataataat cctggtggtg
     3781 ggtttattgg tggtttaata tttagttcag cgtttatttt aatgtttctg gcttttaatg
     3841 ttgaagaggt tttagaaagt ttaccgattg attttagaat tttaatgatt attggagcat
     3901 tggtatcatc tattactgcg ataataccta tgttttttgg aaaaccattt ttgtctcaat
     3961 atgaaacaac ttggatactt ccaattttag gacaaattca tgtaagtaca ataacacttt
     4021 ttgaattagg tattttattc tcagttgttg gtgttattgt cacagtgatg ttgtcgctta
     4081 gcggaggtcg atcatgaatt taatattatt actagttata ggatttttag tgtttatagg
     4141 aacatatatg attttatcaa tcaatttaat tcgtattgta atcggaattt caatatatac
     4201 tcatgctggt aatctcatta ttatgagtat gggaacgtat ggttctagta gatcagaacc
     4261 actaataact ggtggaaacc aattgtttgt tgatcccttg ttacaagcta ttgtactaac
     4321 tgcaatagtt atagggtttg ggatgactgc gtttttactt gtacttgttt atagaactta
     4381 taaagtaaca aaagaagatg aaattgaagg cctaaggggg gaagatgatg ctaagtaact
     4441 tattgatttt accaatgtta ttaccattcc tttgtgcctt aatccttgta tttttaaaaa
     4501 ataatgatcg tatttctaaa tatttatact taggtacaat gactatcacc acaattattt
     4561 cattaatgct attaatttat gttcagcgtc accgtccaat tacgctagac tttggaggat
     4621 ggtcagcgcc ctttggtata cagtttttag gagattcttt aagtttaatt atggttacaa
     4681 ccgcttcgtt tgtgattact ttaattatgg catacggatt tgggcgtggc gaacataaag
     4741 caaatcgtta tcacttgcca tcgttcatat tatttttaag tgttggcgtg ataggctctt
     4801 ttctaacatc agatttattt aatttatacg tcatgtttga aattatgtta ctagcgtcat
     4861 ttgtactcat tacacttgga caatctgtag aacaattacg tgctgcaatt atttatgttg
     4921 tcttgaatat tattggttca tggctattct tattaggtat aggtttactt tataaaacag
     4981 taggtacatt aaacttttca catattgcaa tgcgtttgaa tgacatggga gataatcgca
     5041 ctgttacaat gatttcatta atcttcttag tcgcatttag tgcgaaagca gcgctggtcc
     5101 tttttatgtg gctacccaaa gcctacgctg tgttaaatac tgagcttgca gcattatttg
     5161 cagcgttaat gaccaaagta ggggcctatg cattaattcg attcttcact ttactatttg
     5221 atcaacataa tgatctcata catccattgc tagcaactat ggctgctata actatggtca
     5281 tcggcgctat aggtgtcatt gcttataaag atattaaaaa gattgcagct taccaagtca
     5341 taatctcaat aggatttatc attttaggtt taggaacaaa cacgtttgca ggtattaatg
     5401 gtgcaatatt ttatttggta aatgacattg ttgtaaaaac attgctattt tttattattg
     5461 gtagtttagt ttacattaca ggctatcgac aatatcaata tttgaatggc ttagctaaaa
     5521 aagaaccttt atttggagtt gcgtttatta taatgatttt tgctattggc ggcgtgcctc
     5581 catttagtgg ctttccgggg aaagtactta ttttccaagg tgcattgcaa aatggcaatt
     5641 atattggact agcgttaatg attattacta gtctaattgc aatgtacagt ttatttagga
     5701 tactttttta tatgtatttt ggagataaag atggggagga agttaatttt aagaaaatcc
     5761 cgctatatcg aaaaagaatt ttaagtattt tagtagttgt ggttatcgca atcggaattg
     5821 ctgcacctgt tgtgttaaat gttacaagtg atgcaactga gttgaacacg agtgatcaat
     5881 tatatcaaaa acttgtaaat ccgcatttga aaggagagga ctaaatgaat caaatagttt
     5941 taaatattat cattgcattc ttatgggtat tatttcaaga tgaagatcat tttaaattct
     6001 cgactttctt ttctggatat ctaattggtt taattgtcat ttatatatta cacaggtttt
     6061 tcagcgatga tttttatgtt agaaaaatat gggta
//
LOCUS       DAMQYT010000151         6088 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMQYT010000151 DAMQYT010000000
VERSION     DAMQYT010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.153"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..849)
                     /locus_tag="PGA33_002115"
     CDS             complement(<1..849)
                     /locus_tag="PGA33_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDG6473916.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIVYVIHPINADWLLGSSP
                     YTSLENEGQSIFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRG"
     gene            1002..1421
                     /locus_tag="PGA33_002116"
     CDS             1002..1421
                     /locus_tag="PGA33_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDG6473917.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDIKSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            1448..1558
                     /locus_tag="PGA33_002117"
     CDS             1448..1558
                     /locus_tag="PGA33_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473918.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(1628..5398)
                     /locus_tag="PGA33_002118"
     CDS             complement(1628..5398)
                     /locus_tag="PGA33_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDG6473919.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKS
                     AAKPAALETQYKTAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYK
                     PQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTING
                     EISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASF
                     ARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSH
                     NYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGV
                     AQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVK
                     EGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQI
                     DKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINA
                     KNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVI
                     YNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIY
                     LFGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNN
                     TGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNV
                     QNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVY
                     LYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYS
                     LKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVA
                     QIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISID
                     KINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNK
                     VVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQ
                     NTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(5626..6060)
                     /locus_tag="PGA33_002119"
     CDS             complement(5626..6060)
                     /locus_tag="PGA33_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6473920.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
BASE COUNT         1841 a          975 c         1031 g         2241 t
ORIGIN      
        1 tcctctaaca atgccaataa ttaaaccatg taataaatat acgtatagcg tacgactacc
       61 aatataagta tataattttt tctttgttga cattaaattt agaaacgcag tcattgcgat
      121 taatataatt ccatataata taagtcgttt aaaaggactg aatatactct gtccttcatt
      181 ttcaagtgaa gtatatggtg aacttcccaa taaccaatct gcattgatag gatgaatcac
      241 gtaaacgata aaaaacaaaa taaaggtaat gatagatact ggtattagtt ttttattttt
      301 aaaaatagcc gtatgttttt tggtgaaaat gtaacctaga taaaatattg ggaaaaatac
      361 gattgtcctt gaaatgctta agtagctatc gatgttatct gaaaaacctg ctccaataga
      421 tataataatt gaaactgata gcactttata tggattaaat cttctaacta ttactaaaat
      481 gacatgaaag aaaaatagcg tgatcaaaaa ccataacgca aatactgggt taaaaggatc
      541 aagttgtaat tcgtcacttt tacctgttaa gaaataataa attgaaaaga atgcaaaaaa
      601 tatcatataa ggtactatca aacgttttga aattttttct aaatagtatg gtttatcaat
      661 atttttcgcg aaataaccag atataaacaa aaatgttggc atatggaaac tataaataac
      721 taggtataat gctgataaat atttatcgcc actagtgtag ggttgtaaca tatgtccaaa
      781 tacgactaat aatattaaaa ttgctcttgc gttgtcaaaa aaataatctc tctcttttaa
      841 tgaagtcata ttttatctcc tttatgtcca taagactctt actaatttat tatattttgt
      901 attagttatg caacaaattt gtctattgtg tcgaatagta ttgtaaaaat tataaaaaaa
      961 gcatataata ttataaataa tatgtagtgg agtagaacaa catgtataaa caacttgaaa
     1021 aacttattac actgactaac aatgacttaa acttagtgaa tagaagattt ggacaacgca
     1081 cggatatcaa atctgaacag ctagaacttc tccgtatttt atttaattac gatcgcttat
     1141 cacagtatga tttgacgatg aagattagca gggaacaatc tatagtttca aggtggatta
     1201 agaaattagt tttgaaaggg tacatcacaa gtcaacaatc tagcgaagat ttaagatgta
     1261 aagaattaat tttgactgat caagcacgta cattaatttc acaaataaat aatgcacgtt
     1321 gcgaattgat tgaagcaaga tgtcaatgtt tatcggaagt cgaattagac aatttaaatc
     1381 aattacttga taagttaaat caacgacgca tatcgttgta acacgacaat agcaaacaca
     1441 taatttagtg tcgttgtgca cacaacaaaa attgaatcat gaattacaag caaaagtagc
     1501 ggtgattgtt aaaattgatg gtaaacaatc accgctattt ttgcttgtgt atgtataaaa
     1561 aagggatcaa aggtcatccc ccatgattga tagtggggga tgacttttga tcctatgttc
     1621 atgttgctta tttatattgt gggatgtcga agtatttgcc gacttcgcca attttatcat
     1681 agtagccttt gatgatttta gcattgatgt tagcccaatc tacatctgta gcatattggt
     1741 gtgttcctgg atgtgcagga ttccatctca ttttgtaaag tgtattttga ccagctttta
     1801 catatgagtt gccgatgaat ttagcaccac caacgattgc ttttgatact gtgtcccaac
     1861 cagcttgttt agcatattta ataccttcac gtaaaggatc gttatcatat gcagcaatac
     1921 caaatacgtt atggtatttc gtgtttgagt tagttacaac tttgttgttc actacatctg
     1981 cacctttcgc taattgagaa gtaccgttac ctgtttctaa tagggcatgt gagataagat
     2041 aaacttcatt aatgccatac atttgagcag ctttgttaaa tgcagcacct tggttttcta
     2101 atacaccttt accttttaag aattgattaa ttttatcaat agaaatattt tgtggttggt
     2161 ctaagcgtaa gaattgatat tttaatgctg gatcttgagc taaacgcttc gtatccattg
     2221 catgcttaac atcattaaag ttagcatctg tccacttacc tggtacacgt tgtacttgtg
     2281 gtttatattg taaaccagct tgtatttgag caacttggtt taatgtcata cctgtttgat
     2341 tatacttaat taattcttta gctaaatcag ttgatttaat ccatgctaat ttaccgttag
     2401 ataatttacc atagtaccaa gtttgtccat taatgacttg ttctttaaca actgcgaatg
     2461 gttgttcatt aaatgctttt aatgagtatt tagctgtatc agaatttggt gttacatagt
     2521 aataaccatt accattttta attacataag tgtagttata atctttggca gctgatgtag
     2581 ttggtttcac agcggttggt gcagttaaat cttttgcatt tacccaacca gtgcggttat
     2641 taatagtacc gtataaataa acatctttgc ctacagatac ttgtttcgtt gcattaaatg
     2701 taccttgagc aatgttattg cctgttaaaa tgacttggtt tttagtaccc caaggaacca
     2761 ttgataagcc gttatttgat ttattaacag tatatttttg agtcgtttta acttctttgc
     2821 ctaagttttg aacatttaag tcttttacat tgaaccaacc taatgggatg ttatggcttg
     2881 tattgtttaa taatacatac gtttcattac catgagcacg ctcttttgtt acatagaacg
     2941 tacggtctgc atatttcgca ccgtttttcg ctgttttttc ataaacagaa gcacgaatac
     3001 cagtgttgtt tggtttaact tgagcaatct tgctaactgt ttgagtcgtt tgtggtttag
     3061 taacagtata agcttttaca gctgtttttg gttgtgctac tgctttttta ggtgcagcag
     3121 gtacagctaa atatgcttta cttacccaac cagatttacc atttacagtt ccaaataaat
     3181 agatagattt atcaatttgt tgttgcttag tcgctttaaa agtttggtta cctgtaccag
     3241 aaactgcacc agcttcttgt ttataagtgc cccaaggtac tgaatataat ttagtgcctg
     3301 gttttactgt atatgtttgc attacattta caggtgattt tgcattgtta taaataacgt
     3361 caccttgttt aacccaacca attaaagttg gactattgta atctttaact aagtagaatt
     3421 tgtttccacc taaacttgct tcttttgtta cagcaaatgt tttttgaact tctttcgttg
     3481 gcttaccagt tttgtcataa actgtagtga ataagccatt gtttttagca ttaatttgag
     3541 caacaccgtt taatgatgaa actgttaatt tattatttgt tgtaggtgtt gatggcttag
     3601 gtgttggtgt aggcgtaggt ttagcagtat caactaaata tgctttactt acccaaccag
     3661 atttaccatt cacagagcca tataaataaa ttgatttatc aatttgttgt tgctttgaag
     3721 ccttaaatgt ttggtttcca gagccagaca cactaccagc aacttgttta gatgtacccc
     3781 aaggtactgt ataaagtttc gtaccaggtt tgattgaata tgattgattt acatttacag
     3841 gtgatttagc tgtgttgtaa accacatcgc cttctttaac ccaaccaaat ttattaccag
     3901 aattgtaatc ttgaacaaga tagaattttt gattacctaa tgtagctgtt ttagatacag
     3961 caaatgtttt ttgaacttca ttagttgctt taccagtttt gtcgtataca gtagtatata
     4021 aaccactatt tgttggtttg atttgtgcga caccattgtt tgcagcaact gttaatttac
     4081 cagttgatgg tttcgacggt gttgttggtt ttgatggtgt agtaggggta gttgtagatt
     4141 gcgtacccca tggcgccact ttacccattt ttattaaata tttttcatta attaagtcat
     4201 ataattgatc ataactataa ttatgacttc ttaaatatcc atgtggatcg gcatggtcag
     4261 taccacctaa atatttactt acagcgtagt gagtccatac tgtaccattt ccatcatact
     4321 cagcactgtc tggttttaaa ccataatatt gtaattgtgt agctgcatag tcagcatagt
     4381 tattcattga acgtgcaaat gaagcatagt cgtgtgtgtg tacgatttca acattgatga
     4441 atctagggtt accgactgca ccgacacccc aagataagta atccgttggt gctgtttcga
     4501 ttatacgatc cccatcaaca aatgcatgta cgaatgcgtt ttgatagtta tttttcatat
     4561 aactaatttc accatttatc gtcgaacgat cattagctgt atcatgaact acgatacctt
     4621 caggacgacc tacgccgtta cggtatgcgt atttagggaa gtaagatgta taatcttctt
     4681 caattttagg tgcttttaag ttatttttac gaatgtaatc gttaattgaa gagtttactt
     4741 gtggtttata ttttggcaaa ctcgtttttg gtgttgcagc aactgatctt ggttgtgctg
     4801 aagcgctaaa agtagttact ttaggtgtcg cttcagtttt agctttaggt gctgatgtag
     4861 ttgcagcttt aggtgctgcg gttttatatt gcgtttcaag agctgcaggt ttagcagctg
     4921 atttaattaa ttctggatta atttgatttt ctgaattatc atcttcatca tcaactaaac
     4981 tataaccagc atttgtaaca ttagtgttag ttttaggtgc tgtagtgctt gttgactttg
     5041 caacaggctg cgtattattt gtagtcgctg attgattagc acgagtgtca ccatttactt
     5101 gtgcagtatc aactttttga cttacttgag cattgcctgt tttgttattt gctgtttttg
     5161 gttggacaat agcagggtct tgatatactt gagtgccaga aatgttttgc gttggatttt
     5221 ttacctcagc ttttgcttgt tcagtagttg ctttaacttt attactatct aaaacgtttt
     5281 tattagtagt ttgatcttgt gtcgtctcag ctgcttgaac ttgatgtgca gtgactgctg
     5341 aacctacaag cgttaatgca accattgatg gtagtttgta attgaatttt ttcgccattc
     5401 tatttattac tcctaacatt tattaattat tactaacatt atagtacctg ttttatatac
     5461 ctgtgcgtat ttaaccaatt tgtaatatac tcttaattta cattttcact gtaaaaaaat
     5521 gaaaaaaatt ttgaatctca atggtgacgt gacaaatcaa atagaattga tgtcaattta
     5581 agaattggtt tattacaaat gtttctaaaa tagatacaac tttttttaat taagcgaggt
     5641 taactttttt gtcatttcaa tatgctcgat gccttcctca agaaatatat tacctctcat
     5701 tttaaagttt aaactttcat aaaatgggat agcatgacat tgggcattca tagtagctac
     5761 gtaaaaacct tcatctttag ctaatgattc tacagcttgc ataagcattc tacccattcc
     5821 ttgtccacga tgtgatttca tcacagctac tcgttctatt ttgacagttg tttcattaat
     5881 agggcgtatt cgagcagtgg caactggctg tccattatca tatccaatga ggtgaataga
     5941 ttcagattca tattcatcaa tttcactttc ctcagggacg ccttgttctt ctacaaacac
     6001 tttctttctt atatagaagc aatcttctaa catcttttga ttgtttactt ttgaaaacat
     6061 aaaattcctc cacatcctaa caaaatca
//
LOCUS       DAMQYT010000152         6027 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMQYT010000152 DAMQYT010000000
VERSION     DAMQYT010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.154"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(133..1011)
                     /locus_tag="PGA33_002120"
     CDS             complement(133..1011)
                     /locus_tag="PGA33_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HDG6473921.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            complement(1031..4471)
                     /locus_tag="PGA33_002121"
     CDS             complement(1031..4471)
                     /locus_tag="PGA33_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HDG6473922.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEGLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQVATDNAQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDMSYSTIEDFINSDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQALKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTRVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIINPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLELLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            4775..>6027
                     /locus_tag="PGA33_002122"
     CDS             4775..>6027
                     /locus_tag="PGA33_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDG6473923.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWMIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGAL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     EQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFVSWIKRTKN
                     TAIKVPHRVKKPASISLIICLIVLG"
BASE COUNT         1878 a          985 c          900 g         2264 t
ORIGIN      
        1 atctatcaaa attcggctta tttattcact ctgacatatc caaatacgca aaaagactat
       61 ttctaccttg tatcgataga aatagtcttt ttatacttaa ttggtttaat ttttcataaa
      121 atcttatact ttttaaaaat gggatgaaat atattttcct gaaacatgta attcatgttc
      181 gttacaaatt gataaaatat gattaagcgt tgttgcaaat gacattttct caaaaagcgc
      241 ttcactatca attggtactt gtgtgtgaat ttcagccagt cgcttcgata aatatagttc
      301 atctaaattg tcattaatct tattacgttg cccagctgat aatgcatcaa tgttttcaac
      361 cacattttca acgctttgat attgctgaat taacttaata gccgttttct cgccaatacc
      421 tttaacaccc gcatatccat ctgctgtatc acccataaac gctttaatat cgattagttg
      481 ttgtggttca agggcatatt cttcgttaaa acgatgtaat gtatatctat tataaatgtt
      541 aaaacctttt ttaattagcc aaacttcaac attgtcatta atacattgca gtaaatcttt
      601 gtcgcccgta ataatataga catcgttatc agttgaatat tgttgtgcta atgtacctat
      661 aacatcatcc gcttcatagt ttttaacgcc aatatttaca aagccaaatt gctctgaaat
      721 ttctttaaca taatcaaatt gtggtatcaa ttcttctggt ggtgcagaac gattttgctt
      781 ataaccatca aacatatcat ttctaaaagt tgattgtccc atatcccaac atacagctac
      841 atgtgtaggg cgtatttcat gtattgccga aaagatatga cgcacaaatc cttgtattcc
      901 atttgtaggt tcaccttgtg aattgtacat aaattgttta tgaagacttg tagcatagaa
      961 atgtctaaat aatagcgcca taccatctac aagtaatatt ttattaggca tttaaaacgt
     1021 tccttctttc ttaatataaa atagttttca gttgttgatg tttatctttc aattgattga
     1081 taagtgttgt atctaaatca aatgctaata aatcgtttat ttgcgtttga atattagctt
     1141 caaaactttc aaattgcgat tcagcttcta cagccatttg ctttacatat aattcaagct
     1201 gttgcctcaa taatcccatt tgtggttgta ataattctaa cgtactttga caaattgatt
     1261 catgtatatc tctttgccca tttggattca aaattttacg ttttgttaat tgtttaggca
     1321 atgcatttag catatcgttg aaatcgatgt gcaataatgg ttgctctata ttagctgatt
     1381 caaagttaaa ctgaggatta ataatgacat gtaaatctgc taattgttga acgattggtg
     1441 caatttgctc agtgagttgc ttattaaagt atttttttat acgttctgta attaaagatt
     1501 gctctaaaaa caatcgttga tgaatttgat ctaagtatac tctcgtagac actttctttt
     1561 cttcattaaa atcactattt tgcgtcattt gagaattaaa cactgattta acatcgtcaa
     1621 gtagttgtag ttttaaacgg gcatttaaat gataaacttg ttcttcaact tcgttgtctg
     1681 tatgttgtaa cgtcgcatca attaattgat gttgtaaacg ctgtttatct ttataatctg
     1741 ttaacctttg ttggcgacgt gaaatatcag ctttatttgt ttcaaattct gtaatcatct
     1801 ctacataaga acgatccatt tgttgaagct gatgaatcat ttgttgttct aaaattgatt
     1861 tagattcaac atcaacaaat tgttgtatgc tttgttttaa ttgatcaatg cccttatctt
     1921 cagcttgcaa tgcatttcga cttgatacag caaaaatgtc tgattgtaag tgtacttgtc
     1981 ttaatgcatc tgatacatat gtttcaactg cttcaagatc atcttgactt tctgctaaat
     2041 cagcagcatt aattaccatt tttaatgctt ggttttcatt caactggttc atatctttca
     2101 tgtgttctat aaacgctttg tcattatcag taaatgaatg attaaaataa cttacataca
     2161 atattaagtc tgaagaagtt aaaatttgtt cggtttcatt tgtatgcctt tggttatttg
     2221 agtgtagccc taatgaatca acaattattt taccttttaa ccaatcatgc attaatgcaa
     2281 tgtgtactgt cttaacaaat gttgcatatt catcttctgc actccacttt ttcaattcct
     2341 gttgattaat ggcatgtttt tctccatttt ccaacatatt gacatacaat ttataatgtt
     2401 tttcaactgc ctgaataaaa gcgagttgat ttttatttaa atgtgatttt aacttttcta
     2461 aatctgaatt aataaagtct tctatagtcg aataagacat atcttggtat tcaactactg
     2521 cattaatttc atctaataat tgcgattgtg attttagcgt tatataactc tcgtctccat
     2581 aagatatttc tgtagtagct gctgtagtag gatttggaga actgactaaa atatgctcgc
     2641 ctaataatgc atttatcaaa ctacttttac cagcactaaa tgttccaaat acacctattt
     2701 ttattaattt attatctata cgtgttaatg tttcgtggat atcttgcttt gtacgcttga
     2761 acaaaggcac atccgaaatt atatcaagcg ctttttgaat atctatagac atatttgttg
     2821 tagctgtatt ttgatttagc tgctgattat cacgattatc ctgagcatta tcagtagcca
     2881 cttgatatgt tgtctcttgt cgacctatta atttatctag agattcatct aaatgaatat
     2941 agtagtgacg ataattctta gtcgtcaatg actgacgcag ctcatttaat tctgtataac
     3001 gttgatattc ttttaaatca tcactttctt ctgttggtaa ttcatctgcc tgcacatttt
     3061 ctattatttc tttaaaaatt ggtgttgatt gttgttcaac atatttctta atggctttaa
     3121 ccacttcgtc tgaaaatgta agtacataag tattgctgat tgatgtttgt ggttgatata
     3181 aaccttcaat catttctggc ttaacgtcat aatgctgatt taatacttta tctgaagctt
     3241 cttttttatt gataaaacgc gtaacaaatg acatatcttc tcgcattggt tgacgaattt
     3301 gttgattaac atgttcttgt aacgcatctg tcgctgttaa taatcgctgt tgttgaattt
     3361 gtagtttttt cttcttttta ttaaaaaatc cgccaacatt aaagtcttga gacatacttt
     3421 ccaaataact tcgtaacatt tctctcatat tatgcggcat aatgtatgca ttttctaaaa
     3481 tatttttacg cttattcttt aaaaatgcca ttaattcatc tggattattt aaaagttgtg
     3541 cctcttcact aattgcttga tgttgttgac tatttaaaaa tgcttgttca aactccgctt
     3601 cttcgatacc taaatcttct agtacttcct gaatttcaga ctgaatgtaa tctagctgag
     3661 cttcggtaat gtattcaacc gttcttgatg tataatcctc tattgtctct ctatgttgat
     3721 ctaatgaaat tagataactt gataatactt caagttcatt ttcagggtga tcaaatttag
     3781 atacataaaa ggtgcgttct aatttaatac cccaatctgc aattgatttt tcaactcgag
     3841 atttaaacgt agagaatgac aattcatcgt cttgatgctt gtcaatttga ttaatgataa
     3901 acacaacagg tattccaaca tcatttatat gcttcataaa cttaaagtta agttcagatt
     3961 gaacgtggtt atagtcaacc gtataaaata tcatattact tgtatacata tattgttctg
     4021 ttattgactg atgtgatgca acatttgaat caacacctgg tgtatcttgc aacgtaaacc
     4081 cattttcaaa tttagctgat tgaaaattaa tttctacaga ttcaacgtcg acattttggc
     4141 gattcatttc ccttacttca tcataattag ataatttggc atacgtttga ttcggcaaat
     4201 tagcaataat atcgtgattg tctgaaactg acacaatagc agtattactt gtcgttggta
     4261 caggtgaact tggtaagata tcttgttcaa ttaataaatt tatcagtgtc gatttacctg
     4321 cagaaaaatg tccaacgaac gaacatgtat attgctgcaa atatactttc ttaattactt
     4381 ggttaattgt atgtaaaagt gcttcatttc gcgacttttc aacttctttt tttaatttat
     4441 ataaaagatc taattgttct ttattaatca tgttactttc ccctttggtt cgttcacata
     4501 gaaatgcttg cttgttacaa cgaattcata caatatcgat gtccatttaa acatagatcg
     4561 ttaaatggct cgcattgtat tatgtttaaa ttttatcata gctgcttgat aaaacatacg
     4621 aataatcaat tttcttcaaa cattccaact taaaagttca tttttctaaa aatcacaaat
     4681 catcgttaaa tttttctaga aaataaatat tgtcacagtt tgttcacaca catcacagaa
     4741 ttgacaccgt ataatagcca attgggaggg aaatatgaaa aatacattta atccattaca
     4801 aagattacat ttctatgcag caatatttat tgctccactg ttaatcactt taaccatttc
     4861 gggcattggt tacttgttct ttccagaagt tgaaaataat atttataaga atgagttttt
     4921 tggtgacagt gatgtaaaaa cgcatcaaac attaaatgat gcagtacatc aagttgaaca
     4981 acaatatgaa ggattctttg taagtaaagt tagcatactt gatgaaccat ataacaaacg
     5041 aattacactg agtgatatgg caggaaatca acgttacgtc tttctagatc ataacaatca
     5101 atttgttgca gatcaaaatg cgaaacatac gtattctaat gtgatgcgaa gtatacatag
     5161 ttctttgttt actgaaaata ctattattaa ttatttagta gagttaaccg catgttggat
     5221 gatattcatg attttatctg gtacttattt actcattaag aagcatttaa tttctaacaa
     5281 aagtaaggca cttcgttggc aaaagtggca cgcaatgatt ggagttatca ttgcaattcc
     5341 agtatttgta ttagtcttaa ctggattgcc atggtctggt tttatgggca gtaaaattgc
     5401 cggtatgatg gacacaaacg gtgcccttgg tcaaggtgaa ttagcgatta atccacctaa
     5461 atcagatttg aacgaattac cttgggctac acgtaaaaat aaacagccag cttcatccga
     5521 aaaaggttca agtggtcatc atggtaatgc agcaatgcct caaaccaaat tagattatca
     5581 aatatctatt gataaggtcg ttgaacaggc gcaaaaagct ggtattaaaa agccgttttc
     5641 aatcgtatat ccaagtgata aaaatggtac ctttattgta tctaatacta gtaattcagg
     5701 tgttactggg ctagatgtat caccatacaa ggaacaaaca ctttatttcg atcaatatag
     5761 cggtaaaaag ctaggtacga ttaaatatga tgactacggt attattgcta aatggtttac
     5821 atggggcatt ccgcttcacg aaggtcattt attcggcatt ttaaataaaa tcattaattt
     5881 atttgtatgt atcgctttat tagtagccat tggcatgggg tttgtctctt ggataaagcg
     5941 tacaaaaaat actgcaataa aagtaccaca tcgcgtaaaa aaaccagcat ctatatcact
     6001 cataatatgt ttaattgtat taggatt
//
LOCUS       DAMQYT010000153         6024 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMQYT010000153 DAMQYT010000000
VERSION     DAMQYT010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.155"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            107..1300
                     /gene="fmtA"
                     /locus_tag="PGA33_002123"
     CDS             107..1300
                     /gene="fmtA"
                     /locus_tag="PGA33_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HDG6473924.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRP
                     NILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKKYPDEYRYGFY
                     AKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
     gene            complement(1837..2115)
                     /gene="qoxD"
                     /locus_tag="PGA33_002124"
     CDS             complement(1837..2115)
                     /gene="qoxD"
                     /locus_tag="PGA33_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HDG6473925.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(2124..2729)
                     /gene="qoxC"
                     /locus_tag="PGA33_002125"
     CDS             complement(2124..2729)
                     /gene="qoxC"
                     /locus_tag="PGA33_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HDG6473926.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(2719..4707)
                     /gene="qoxB"
                     /locus_tag="PGA33_002126"
     CDS             complement(2719..4707)
                     /gene="qoxB"
                     /locus_tag="PGA33_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HDG6473927.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(4707..5807)
                     /gene="qoxA"
                     /locus_tag="PGA33_002127"
     CDS             complement(4707..5807)
                     /gene="qoxA"
                     /locus_tag="PGA33_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HDG6473928.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
BASE COUNT         2151 a          964 c         1030 g         1879 t
ORIGIN      
        1 aaatgaatga taagttaatt tttgtaagtt gtattattag gcatctaggt tcaaattttt
       61 aacgatttaa atatataaaa tgaatacatt gtgaggcaat tatcaaatga aatttaataa
      121 agtaaaacta gttatacatg cgtgtgtact attatttatc attatttcta tagcgttaat
      181 ttttcatcga ttacagacga agacacattc tatagaccca atacataagg aaacaaaatt
      241 atcagacaat gaaaaatatt tagtggatcg taataaggaa aaggttgcgc cgtctaaact
      301 aaaagaggta tataatagca aggatcctaa atataagaaa attgacaagt atttacaaag
      361 ttcattattt aacggttcag tagctatata tgaaaatggc aaattgaaaa tgagtaaagg
      421 ttatggatat caagattttg aaaaaggtat taaaaacaca ccgaatacga tgtttttaat
      481 aggttcagct caaaaatttt caacagggtt actgttaaaa cagttagaag aagaacataa
      541 aataaatatc aatgatccag taagtaaata ccttccatgg tttaaaacat ctaagcctat
      601 cccattgaaa gatttaatgt tgcatcaaag tggattatat aaatataaat cctcaaaaga
      661 ttataaaaat ttagatcaag cagttaaagc gattcaaaaa cgtggtattg atcctaagaa
      721 atacaaaaag catatgtata acgatgggaa ttatttagta cttgcgaaag taattgaaga
      781 agttacaggt aaatcttatg ctgagaatta ttatacaaaa ataggagatc ctttaaaact
      841 tcagcacaca gcattttatg atgaacaacc ttttaaaaaa tatctagcaa aaggttatgc
      901 ttataatagt acaggacttt cattcttaag acctaatatt ttggaccaat actatggtgc
      961 aggtaattta tatatgacac caacagatat gggtaaatta attactcaaa tacaacaata
     1021 taaattattc agtcctaaaa taaccaatcc attattacat gagtttggta cgaaaaagta
     1081 tccagatgaa tatcgatatg gtttctatgc taagccaaca ttgaatagac ttaacggggg
     1141 attctttgga caagtcttta ctgtttacta taatgataag tatgtagttg tacttgcatt
     1201 aaatgtaaaa ggaaacaatg aagttcgaat caaacatatt tataatgata ttttaaaaca
     1261 aaataaacct tacaatacga agggtgttat tgttcaataa ttaatataga agatataaca
     1321 tgtatatggc attaaggcat cgaccttatc tgaccagtat acgagttata tcttcttttt
     1381 tatagtggta aaaagtttaa agtataaggt tgaagaagga tgagtttaaa aatatgtgtt
     1441 aactgataaa aggggaaatc atttggtgag ttggcatcag actaaaatga atgaagacga
     1501 atacgttggt ccatgcgtgg tgtgaatgtt ttctaaatct aactggaaat gataatttaa
     1561 taatgtacac ttttgattgt ttaaacatgt acaattttta aaaacctagg gataatttta
     1621 ttgacgtgaa caacattgag attatcttta gacataaata tggattttga agttactata
     1681 tttattagtt gtggttgtta atgtattaat tgaattgatt ataatgattg tcttataaga
     1741 tatagaaggg gacttaacca tttaaaaatt gaaggaaaat aaaaaccagc taacaaaagt
     1801 gttagctggt taaactttac attattaaaa tgttgtttat aagtgtgaag agtgaccgcc
     1861 ttgcataacc caatatgttc cgacaacgaa acaaagtgta attacaagag caaagataac
     1921 tttgaatgtt tgtaaacgtc catctttacc ttcagttaaa tgcatgaaca ttaataattg
     1981 aagtcctgct tggacgaatg caaagccaaa gataattgtc aacttcgcgt ggaatgttaa
     2041 tgacgtgtat agtgttacgt aaactgctaa aagcgttaat acgatagatg cgataaatcc
     2101 tacagtatgt ttcattattg tactcatccg ctatacacca tccctatcat atatacggca
     2161 gtaaagatga aaacccaaac aacatctaag aagtgccagt ataaacttac tataaataat
     2221 tttggcgcat tatatttgtc taatccgcgt cgttggattt ggattaataa acaaatggcc
     2281 caaacgatac ctagcgatac gtgacaacca tgcgttccta ataggataaa gaaactagac
     2341 cagtaagaac caattgttgg gttaacgcct tctgatgcat agtgtgcgaa ttcataaatt
     2401 tcgaatccaa caaagactaa acctaaaagt aacgtaatga tcatccaaaa catcattaac
     2461 ttttgttttt cttggcgcat gtagtaaata gcaataccac atgtgtaaga actgaataat
     2521 aatgcaaacg tcattattaa aacaagaggc aattcaaata actcagtagt cattttacct
     2581 gcataatcgc caccatgttg caaagttaat agtgtcgcaa atagggtacc gaataacgca
     2641 aattcggctg taatgaaaat ccaaaagcca agcttattta attcgccttc atgtgtgcgt
     2701 gaatcaatag tgtttgtatc atgactcatg acttacagcc tccctttctt taatacgcgc
     2761 ttctcttaat ctagcttcag tttctgcaac ttcagcagca gggatatgat atccgtgatc
     2821 gatttggaaa ctgcgataaa tcatagtacc aaaaataccg aataaacaaa ttaatgctgg
     2881 aattacagtt tcgaaaatta agaagaaacc gccgatagtc ataaagatac caatccagaa
     2941 tccaacagga gtattgtttg gcatatgaat gtctttgtaa ttatggttgt ctaagtaatg
     3001 acgaccatgt tctttcatat caacaaatgt gtcgtagtca ttccaatctg gtgtaatggc
     3061 aaagttgtat ttaggtggaa ttgctgatgc agtagtccac tctagagtac gaccaaggcc
     3121 atcccagtta tctccagttg cttcacgtgg agatttgaag tgactgtata cgatactaac
     3181 aacaaggaat aagaatccga ttgccattaa taatgcaccg atagttgaaa ttaagtttaa
     3241 taagaaccaa ccatctgatg gcatataagt gtataaacga cgtggcatac catctaatcc
     3301 aagaatgaat tgtggtaaga aacaaacgtt aaatccgatc atgaagaacc agaagcacca
     3361 tttgtttaat gtttcgttta atttgtaacc catcatcttt ggataccaga agattaaacc
     3421 agctaagcag gcaaatacaa caccagtaac caatgtatag tggaagtgag ctactaagaa
     3481 gtacgtattg tgatattgat agtcagctga tgccattgct aacattacac ccgtaacacc
     3541 acctaaaagg aagttaggga taaatgctaa tgagaatagc attggtgact caaatgtaat
     3601 acgtccttta tataatgtta ataaccagtt aaacaatttc acaccagttg gaataccaat
     3661 cagcattgtt gaaattgaga agaatgagtt gattaacgca ccattaccca ttgtgaagaa
     3721 atggtgaacc caaactaaga aactaaggaa cgcgatgccg gcagttgccc ataccatact
     3781 ttgatgtccg aataaacgct tacgagcgaa tgtcgggata atttctgagt aaataccaaa
     3841 tgctggaagg ataacgatat aaacttcagg gtgcccccat acccagaaga agttagccca
     3901 aagcattggc ataccgccat gtgcaactgt gaagaatgct gtgtcaaata ttctatcagt
     3961 tgtcattaat gctaacgcta ctgttaaagg agggaaagca agaataacaa ttaatgtagt
     4021 aataaatgtt gttactgtaa acattggcat ttgcataaac ttcatagttg gtgttttaca
     4081 tcttaaaatt gttacaaaga agttgatacc tgtagctaag gtaccaagcc ctgaaatttg
     4141 tatagctatt aagtaatagt taacacccgg accaggactg aattcacctg ctagtggcgc
     4201 atagtttgtc caaccagctg ctggtgaacc accaataata aatgacaggt tgaataaaat
     4261 catacctgca aagaatagcc agaaacttac gttgtttaat actgggaatg caacatcacg
     4321 tgctccaatt tgtaatggaa caacgatatt ccataaacca aagataaatg gcattgccat
     4381 gaagataatc atgattacac catgtgtact aaaaatttcg ttatagtggt tagattctaa
     4441 aaatttgtta tcaggtactg ttaattgcgc acgaataagt aacgcatcaa taccaccacg
     4501 gacgaacatt aatacggcac agattaaata cataataccg attttcttat ggtctacaga
     4561 tgtgaaccat tctttgtaaa gatatttcca taatttaaag taagtaatta ctgcgattaa
     4621 accaataact aagaatgggg caccaatttg tgccattgta atcatccagt tacctttaac
     4681 tagtaattga tcccatggaa aattcattaa tgtccacctc catgatcatc attgtcttga
     4741 tcttgcgcat cttttgaaat tttcttcatt tctttcgcat ttttcgattc atctttcttg
     4801 aactcattgt tatatggttc gtcatttcca agaatcatca acttcatacc atgtcgttta
     4861 tagttcgcat ttgtaatttg agctttacga gcaggtatta atggtttgtc tgatacatct
     4921 ttaaacatat tttcttcact agtgaagttt ggatctttta attcgaaatt gaaacgttta
     4981 tatgcataga agatgtattc tggatcggct gctggatcaa caaacgccat atgtgtacca
     5041 ttaaattcta aagctttatt aggtgtgctt ggtaataatt gtttatcaaa tgtatcttga
     5101 tctaacgttt tcttaccttt aacttctttc acccatttgt cgtagtcttt ttgactaacg
     5161 gcatttactt taaatgtttg acgtgagaat ccttcaccat tgaagttaga gttacgacct
     5221 ctgaacgtac cagtttgaga tgcttctaac gtccaattca ttgtcatgcc agtcatggca
     5281 tatttttgac cacctaattg tggaatccag aaacttgtca ttgtatccat agcttgaagc
     5341 ttaaatacaa caggacgatc tttagggatt gttaatgtat taacagtctc tatatgttca
     5401 tctggataag caaagaacca tttgtatcct gcacttactg catatacaac cattggatct
     5461 ttctcactct tcggtggttt ttcgtaatcg tataaagttt taactgtagg aatagctaaa
     5521 gcagcaacga ttatgatagg tattacaaac catattgttt caatgatggc attatggtgc
     5581 atcttaccag attcggcatt cttattataa ctatacttgt aaataaaaat ggcgaacatg
     5641 ccaagtacaa cgaaacaaat aacaagcatg aagacgattg aataaagaat caagaacttc
     5701 tgactacttg ctactggccc ttttgcgttg aaaatttcta tatttgaaca accactaagt
     5761 aaaattagtg tgccaaataa tagaagcaaa gacttaaatt ttgacacttt tttgacctcc
     5821 taatactaca aatgtagggc ttaacattaa ttttaagtta ttacacaata tttacaaggg
     5881 cttatgggaa aaaaattaat aaaattgtat caaaaatgtt gataaatcaa ggtgtgacgt
     5941 gggttcacac atttgttaaa attatgtgta cattttgtga ctaatagcgt ttttgaaaaa
     6001 attgcttcat atttaaaagg aaag
//
LOCUS       DAMQYT010000154         5944 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMQYT010000154 DAMQYT010000000
VERSION     DAMQYT010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.156"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            650..1561
                     /gene="rapZ"
                     /locus_tag="PGA33_002128"
     CDS             650..1561
                     /gene="rapZ"
                     /locus_tag="PGA33_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDG6473929.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            1558..2553
                     /locus_tag="PGA33_002129"
     CDS             1558..2553
                     /locus_tag="PGA33_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDG6473930.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            2663..3607
                     /gene="whiA"
                     /locus_tag="PGA33_002130"
     CDS             2663..3607
                     /gene="whiA"
                     /locus_tag="PGA33_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDG6473931.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            4181..4768
                     /gene="clpP"
                     /locus_tag="PGA33_002131"
     CDS             4181..4768
                     /gene="clpP"
                     /locus_tag="PGA33_002131"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDG6473932.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(5011..5913)
                     /locus_tag="PGA33_002132"
     CDS             complement(5011..5913)
                     /locus_tag="PGA33_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDG6473933.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKALFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLIKE"
BASE COUNT         2111 a          859 c         1107 g         1867 t
ORIGIN      
        1 tttattatgt agtggttctt aaacattagc cacagctaat gtgtacttaa aaataggaat
       61 acatgagtaa aactcatgca taagaaatac taatttctat agaaaaagta ttactttatc
      121 gttgtcccac cccaacttgc acattattgt aagctgactt tccgccagct tctgtgttgg
      181 ggccccgcca acttgcacat tattgtaagc tgacttttcg tcagcttctg tgttggggcc
      241 ccgccaactt gcattgtctg tagaaattgg gaatccaatt tctctatgtt ggggcccaca
      301 ccccaactcg cattgcctgt agaatttctt ttcgaaattc tctgtgttgg ggcccacacc
      361 ccaactcgca ttgcctgtag aatttctttt cgaaattctc tgtgttgggg cccctgacta
      421 gagttgaaaa aagcttgttg caagcgcatt ttcattcagt caactactag caatataata
      481 ttatagaccc taggacattg atttatgtcc caagctcctt ttaaatgatg tatattttta
      541 gaaatttaat ctagacatag ttggaaataa atataaaaca tcgttgctta attttgttat
      601 agaacattta aattaacatc atgaaattcg ttttggcggt gaaaaaataa tggataataa
      661 tgaaaaagaa aaaagtaaaa gtgaactatt agttgtaaca ggtttatctg gcgcaggtaa
      721 atctttggtt attcaatgtt tagaagacat gggatatttt tgtgtagata atctaccacc
      781 agtgttattg cctaaatttg tagagttgat ggaacaagga aatccatcct taagaaaagt
      841 ggcaattgca attgatttaa gaggtaagga actatttaat tcattagttg cagtagtgga
      901 taaagtcaaa agtgaaagtg acgtcatcat tgatgttatg tttttagaag caagtactga
      961 aaaattaatt tcaagatata aggaaacgcg tcgtgcacat cctttgatgg aacaaggtaa
     1021 aagatcgtta atcaatgcaa ttaatgatga gcgagagcat ttgtctcaaa ttagaagtat
     1081 agctaatttt gttatagata ctacaaagtt atcacctaaa gaattaaaag aacgcattcg
     1141 tcgatactat gaagatgaag agtttgaaac ttttacaatt aatgtcacaa gtttcggttt
     1201 taaacatggg attcagatgg atgcagattt agtatttgat gtacgatttt taccaaatcc
     1261 atattatgta gtagatttaa gacctttaac aggattagat aaagacgttt ataattatgt
     1321 tatgaaatgg aaagagacgg agattttctt tgaaaaatta actgatttgt tagattttat
     1381 gatacccggg tataaaaaag aagggaaatc tcaattagta attgccatcg gttgtacggg
     1441 tggacaacat cgatctgtag cattagcaga acgactaggt aattatctaa atgaagtatt
     1501 tgaatataat gtttatgtgc atcataggga cgcacatatt gaaagtggcg agaaaaaatg
     1561 agacaaataa aagttgtact tatcggtggt ggcactggct tatcagttat ggctagggga
     1621 ttaagagaat tcccaattga tattacggcg attgtaacag ttgctgataa tggtgggagt
     1681 acagggaaaa tcagagatga aatggatata ccagcaccag gagacatcag aaatgtgatt
     1741 gcagctttaa gtgattctga gtcagtttta agccaacttt ttcagtatcg ctttgaagaa
     1801 aatcaaatta gcggtcactc attaggtaat ttattaatcg caggtatgac taatattacg
     1861 aatgatttcg gacatgccat taaagcatta agtaaaattt taaatattaa aggtagagtc
     1921 attccatcta caaatacaag tgtgcaatta aatgctgtta tggaagatgg agaaattgtt
     1981 tttggagaaa caaatattcc taaaaaacat aaaaaaattg atcgtgtgtt tttagaacct
     2041 aacgatgtgc aaccaatgga agaagcaatc gatgctttaa gggaagcaga tttaatcgtt
     2101 cttggaccag ggtcattata tacgagcgtt atttctaact tatgtgtgaa tggtatttca
     2161 gatgcgttaa ttcattctga tgcgcctaag ctatatgttt ctaatgtgat gacgcaacct
     2221 ggggaaacag atggttatag cgtgaaagat catatcgatg cgattcatag acaagctgga
     2281 caaccgttta ttgattatgt catttgtagt acacaaactt tcaatgctca agttttgaaa
     2341 aaatatgaag aaaaacattc taaaccagtt gaagttaata aggctgaact tgaaaaagaa
     2401 agcataaatg taaaaacatc ttcaaattta gttgaaattt ctgaaaatca tttagtaaga
     2461 cataatacta aagtgttatc gacaatgatt tatgacatag ctttagaatt aattagtact
     2521 attcctttcg taccaagtga taaacgtaaa taatatagaa cgtaatcata ttatgatatg
     2581 ataatagagc tgtgaaaaaa atgaaaatag acagtggttc taaggtgaat catgttttaa
     2641 ataagaaagg aatgactgta cgatgagctt tgcatcagaa atgaaaaatg aattaactag
     2701 aatagacgtc gatgaaatga atgcaaaagc agagctcagt gcactgattc gaatgaatgg
     2761 tgcacttagt ctttcaaatc aacaatttgt tataaatgtt caaacggaaa atgcaacaac
     2821 ggcaagacgt atttattcgt tgattaaacg tgtctttaat gtggaagttg aaatattagt
     2881 ccgtaaaaaa atgaaactta aaaaaaataa tatttatatt tgtcgtacaa agatgaaagc
     2941 gaaagaaatt cttgatgaat taggaatttt aaaagacggc atttttacgc atgaaattga
     3001 tcattcaatg attcaagatg acgaaatgag acgcagttac ttgagaggag cttttctggc
     3061 aggtggctca gtgaataacc ctgaaacatc ttcgtaccat ttggaaattt tttctcaaaa
     3121 tgagagtcat gcagaaggct taacgaaact aatgaatagt tatgagttga atgccaaaca
     3181 tttagagcga aaaaaaggaa gtattacgta tttaaaagaa gcggaaaaga tttcggattt
     3241 tcttagtttg ataggtggct atcaagcgtt attaaaattt gaagacgtac gtattgtaag
     3301 agatatgcgt aattctgtta accgactcgt taattgtgaa acggccaatc taaataaaac
     3361 agttagtgct gcgatgaaac aagttgagag cattaaattg attgataaag aaattggtat
     3421 tgaaaattta ccagacaggt tgagagagat tgctagaatt cgagtagaac atcaagaaat
     3481 ttcgttgaaa gagcttggag aaatggtatc aactggtcca atttcaaaat caggtgtaaa
     3541 tcaccgatta agaaaactga atgatttagc cgataagatt agaaatggtg aacaaataga
     3601 attataagta agaaggtgtt tttggtagtg aattatcaaa aacacttttt ttatgattaa
     3661 aaagtgttta atgaataata gtcgactaac tattaaattg agctggaaag tttaatgaag
     3721 gaaattcaat aaccaagtga tgcctaattt tctcattaaa tagcatcata tgtatcaatt
     3781 tggtcataag aaaatatgaa tgtgataatt aattgagtcc ctgaaagtcc ctgatcatag
     3841 acaaaaacaa aatcagcata aataagaatc ccgacattgc gggattctct gtattgaaaa
     3901 gtattgtatt ttattaaaac agcctccttg aagggaattg aacccctatc ttaagaaccg
     3961 gaatcttacg tgttatccat tacaccacaa ggagcaaata tgtaatcata tctctgtact
     4021 atgagaagta aaataagtac aatctaaaat agatattatc agtttacaaa ggaaagagaa
     4081 aagcgtcaaa caatgtaact atttaaagtc aaagtgtttg accaaatttg acttaatatg
     4141 taaaataatg agtaacagtt attacaagga ggaaatatag atgaatttaa ttcctacagt
     4201 tattgaaaca acaaaccgcg gtgaacgtgc atatgatata tactcacgtt tattaaaaga
     4261 ccgtattatt atgttaggtt cacaaattga tgacaacgta gcaaattcaa tcgtatcaca
     4321 gttattattc ttacaagcgc aagactcaga gaaagatatt tatttataca ttaattcacc
     4381 aggtggaagt gtaacagctg gttttgcgat ttatgataca attcaacaca ttaaacctga
     4441 tgttcaaaca atttgtatcg gtatggctgc atcaatggga tcattcttat tagcagctgg
     4501 tgcaaaaggt aaacgtttcg cgttaccaaa tgcagaagta atgattcacc aaccattagg
     4561 tggtgctcaa ggacaagcaa ctgaaatcga aattgctgca aatcacattt taaaaacacg
     4621 tgaaaaatta aaccgcattt tatcagagcg tactggtcaa agtattgaaa aaatacaaaa
     4681 agacacagat cgtgataact tcttaactgc agaagaagct aaagaatatg gcttaattga
     4741 tgaagtgatg gtacctgaaa caaaataatt caaagtaaag agtagactaa gctgtctgct
     4801 ctttttgtat gagtaaacca aggtgtcaat aatttgttta ctatactttg agcggaaata
     4861 tgattgaatg aagctagttg aaccgtaact atatgaaatg ttcccttcaa agtagacatt
     4921 gaaaggaaca tttcaatcct ttgtttgtaa gtcgctctag acattacatt tagtacatat
     4981 gttgtttcta atgctcatta atggtattga ttattcttta attaaatctt caagtgccat
     5041 ttttaaatta ctatatttaa attggaatcc caatgcttga attttattag gtaatacttt
     5101 ttgagtatcc aatactactg ttgacatttg accaagtatg agacgcattg caagacttgg
     5161 tgcccaagtt tcatgaggct tatgcatagc tcttgctaaa gtgtagccaa ataaattttg
     5221 acgttcaggt ataggtgcag ttaaattaaa cggaccacta gctgactcgt tatttattaa
     5281 aaataaaata gcttgaatta aatcattgat atgaatccat gaataccatt gttgaccaga
     5341 acctaattta ccaccaatgt aatattcgta tggtagtttc attgtttgta acgcaccgcc
     5401 ttcattcgat aaaattatac cgaaacgacc gatgacaact cgtgtaccta attgttcaaa
     5461 ttgttgtgcg aaacgttccc attgatacac aatatctgat aagaaatcaa aaggtaaagt
     5521 tttataaact tctgtgtaac tcataaataa atcaggagga tagtaaccag tggcactagc
     5581 attaaataaa gctttaggtg ctttattacg tgatttaaac aattcatata aagcttgcgt
     5641 agattgaatt ctacttagca ttagcgtttg tttatattcc ggtgtccatc gtttattcaa
     5701 tgtagcacct gctaagttga tgaccacatc gatattttga ggaactttgt gttcccaccc
     5761 agatttagcc cagttgacat atgaaatttt cttatcattt gaaatttggt cgtgtcgcgt
     5821 taatatcgtg atatgtgaat ctgatttttt aatttcatta actaattgag atccaaccat
     5881 accagtccca ccagtaatta agtattgttt cattatcatt caccccatgt aattttgtat
     5941 ttag
//
LOCUS       DAMQYT010000155         5934 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMQYT010000155 DAMQYT010000000
VERSION     DAMQYT010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.157"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            289..2538
                     /gene="pflB"
                     /locus_tag="PGA33_002133"
     CDS             289..2538
                     /gene="pflB"
                     /locus_tag="PGA33_002133"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDG6473934.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            2561..3316
                     /gene="pflA"
                     /locus_tag="PGA33_002134"
     CDS             2561..3316
                     /gene="pflA"
                     /locus_tag="PGA33_002134"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDG6473935.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            3652..5415
                     /locus_tag="PGA33_002135"
     CDS             3652..5415
                     /locus_tag="PGA33_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HDG6473936.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWTEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDIVGLKMRQNGHEAKFVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(5579..5923)
                     /locus_tag="PGA33_002136"
     CDS             complement(5579..5923)
                     /locus_tag="PGA33_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HDG6473937.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKQRLESIY
                     NSISNPLHSQNN"
BASE COUNT         2054 a          905 c         1102 g         1873 t
ORIGIN      
        1 agtgttgatt taacgcttat caacaatcat tttttataaa caaatatata ctcctaaatt
       61 aacttttaaa gcaatgaaaa tagtgaacat tataactgtt gtgtaacaga atgcaattag
      121 catattactg ttacacaaat tagtacagtt tctatgtttt gacatacatt tgatgaaaat
      181 tgtacataat ttatgtgaaa aaaatcacaa caaacatgct acaatgacta tgaaaacgtt
      241 aacatagcat ttcaaattca caacattata cagatggagg cgtttagtat gttagaaaca
      301 aataaaaatc atgcaacagc ttggcaagga tttaaaaatg gaagatggaa cagacacgta
      361 gatgtaagag agtttatcca attaaactac actctttatg aaggtaatga ttcattttta
      421 gcaggaccaa cagaagcaac ttctaaactt tgggaacaag taatgcagtt atcgaaagaa
      481 gaacgtgaac gtggcggcat gtgggatatg gacacgaaag tagcttcaac aatcacatct
      541 catgatgctg gttatttaga caaagattta gaaacaattg taggtgtaca aactgaaaag
      601 ccattcaaac gttcaatgca accattcggt ggtattcgta tggcgaaagc agcttgtgaa
      661 gcttacggtt acgaattaga cgaagaaact gaaaaaatct ttacagatta tcgtaaaaca
      721 cataaccaag gtgtattcga tgcatattct agagaaatgt tgaactgccg taaagcaggt
      781 gtaatcactg gtttacctga tgcatacgga cgtggacgta ttatcggtga ctatcgtcgt
      841 gtagctttat atggtgtaga tttcttaatg gaagaaaaaa tgcacgactt caacacgatg
      901 tctacagaaa tgtcagaaga tgtaattcgt ttacgtgaag aattatcaga acaatatcgt
      961 gcattaaaag aattaaaaga acttggacaa aaatatggtt tcgatttaag ccgtccagca
     1021 gaaaacttca aagaagcagt tcaatggtta tacttagcat accttgctgc aattaaagaa
     1081 caaaacggtg cagcaatgag tttaggtcgt acatcaacat tcttagatat ctatgctgaa
     1141 cgtgacctta aagcaggcgt tattactgaa agcgaagttc aagaaattat tgaccacttc
     1201 atcatgaaat tacgtattgt taaatttgct cgtacacctg attacaatga attattctct
     1261 ggagacccaa cttgggtaac tgaatctatc ggtggtgtag gtattgacgg acgtccactt
     1321 gttacgaaaa actcattccg tttcttacac tcattagata acttaggtcc agctccagaa
     1381 ccaaacttaa cagtattatg gtcagtacgt ttacctgaca acttcaaaac atactgtgca
     1441 aaaatgagta ttaaaacaag ttctatccaa tatgaaaatg atgacattat gcgtgaaagc
     1501 tatggcgatg actatggtat cgcatgttgt gtatcagcga tgacaattgg taaacaaatg
     1561 caattcttcg gtgcacgtgc gaacttagct aaaacattac tttacgctat caatggtggt
     1621 aaagatgaaa aatctggtgc acaagttggt ccaaacttcg aaggtattaa cagcgaagta
     1681 ttagaatatg acgaagtatt caagaaattt gatcaaatga tggattggct agcaggtgtt
     1741 tacattaact cattaaatgt tattcactac atgcacgata aatacagcta tgaacgtatt
     1801 gaaatggcat tacatgatac agaaattgta cgtacaatgg caacaggtat cgctggttta
     1861 tcagtagcag ctgactcatt atctgcaatt aaatatgcac aagttaaacc aattcgtaac
     1921 gaagaaggtc ttgtagtaga ctttgaaatc gaaggcgact tccctaaata cggtaacaat
     1981 gacgaccgtg tagatgatat tgcagttgat ttagtagaac gcttcatgac taaattacgt
     2041 agtcataaaa catatcgtga ttcagaacat acaatgagtg tattaacaat tacttcaaac
     2101 gttgtatacg gtaagaaaac tggtaacaca ccagacggac gtaaagctgg cgaaccattt
     2161 gctccaggtg caaacccaat gcatggccgt gaccaaaaag gtgcattatc ttcattaagt
     2221 tctgtagcta agatccctta cgattgctgt aaagatggta tttcaaatac attcagtatc
     2281 gtaccaaaat cattaggtaa agaaccagaa gatcaaaacc gtaacttaac tagtatgtta
     2341 gatggttacg caatgcaatg tggtcaccac ttaaatatta acgtatttaa ccgtgaaaca
     2401 ttaatagatg caatggaaca tccagaagaa tatccacagt taacaatccg tgtatctggt
     2461 tacgctgtta acttcattaa attaacacgt gaacaacaat tagatgtaat ttctcgtaca
     2521 ttccatgaaa gtatgtaaca aaatttaagg tgggagcact atgcttaagg gacacttaca
     2581 ttctgtcgaa agtttaggta ctgtcgatgg accgggatta agatatatat tatttacaca
     2641 aggatgctta cttagatgct tgtattgcca caatccagat acttggaaaa ttagtgagcc
     2701 atcaagagaa gtcacagttg atgaaatggt gaatgaaata ttaccataca aaccatactt
     2761 tgatgcatcg ggtggcggtg taacagtcag tggtggcgaa ccattgttac aaatgccatt
     2821 cttagaaaaa ttatttgcag aattaaaaga aaatggtgtg cacacttgct tagacacatc
     2881 ggctggatgt gctaatgata caaaagcatt tcaaaggcat tttgaagaat tacaaaaaca
     2941 tacagacttg atattattag atataaaaca tattgataat gacaaacata ttagattgac
     3001 aggaaagcct aatacacaca tccttaactt cgcgcgcaaa ctgtcagata tgaaacaacc
     3061 tgtatggatt cgacatgtcc ttgtgcctgg ttattctgat gataaagacg atttaattaa
     3121 actaggggaa tttattaatt ctcttgataa cgtcgaaaag tttgaaattc tgccatatca
     3181 tcagttaggt gttcataagt ggaaaacatt gggcattgca tatgaattag aagatgtcga
     3241 agcgcccgat gatgaagctg ttaaagcagc ctaccgttat gttaacttca aagggaaaat
     3301 tcccgttgaa ttataaatac aattcagacc gaaaagaaag catatgcaac ttcaagagtg
     3361 aaggggcata tgcttctttt tcaattgagt attgagtatt agcaagacgt agtaagtata
     3421 tgagacaact tctacaatgg ttgaaggaag acgtttttgt aagtagctat gctgataaag
     3481 aatgtgatgt cttgttaaag gtggggttcc aatatcatca tttagctgat gttgaatggg
     3541 ttattatttg ctacttgcat atgaatatga gtcttttcaa atttttattg accctgagta
     3601 atgaaaaata ttaagatgaa acttaatatt aaagcaatgc ggagcgtgat tatgaagaga
     3661 attagtaaag atatatgggc agtatttaaa ttactgtatc aaaataaagg gcgttttagc
     3721 attaatgcct tactattgca gttaatcatg atttttatta gtagtacata cttaatttta
     3781 ctatttaata tgatgttaaa agtagctggg caaagccaac ttacgattaa caattggacg
     3841 gaaatcgtta gtcatcccgc cagtgtgata cttcttatta tattcatatt aagtgttgcc
     3901 tttctgattt atgtagagtt ttcattgtta gtttatatgg tttatgccgg ctttgatcga
     3961 cagattatta catttaaatc catttttaaa aatgcctttg taaatgtgcg taaactcata
     4021 ggtgtaccag ttattttctt tgtcatttat ttaatgttaa tgatacccat tgccaaccta
     4081 ggactaagtt cagtattaac aaaaaatatt tacataccta aatttttaac ggaagaactt
     4141 atgaaaacga cgaaaggtat aatcatttac ggtaccttta tgattgctgt atttatatta
     4201 aattttaaat taatatttac tctaccgtta acgattttaa accgccagtc gttatttaaa
     4261 aatatgagac taagttggca aattacgaag cgaaataagt ttcggcttgt tatagaaata
     4321 gttatattag aactcatcat tggtgcgatt ttaacattaa ttatttcagg agcaacatat
     4381 cttgctattt gtgtagatga agaaggagat aagtttttag tctcatcaat tttatttgtt
     4441 gtattgaaaa gcgcattgtt cttctattat ttatttacga aattatcatt aatcagtgtg
     4501 ttagtactgc acttaaaaca agagaatgta ttagaccaac cgggcttaga atttaaatat
     4561 ccaaaaccga aacggaagtc taggttcttt ataatttcaa tggtgcttgc agtgacatgt
     4621 tttatcggtt ataacatgta cttactttac aataatacta tcaatacaaa tatctccatt
     4681 attggccatc gtggtttcga agataaaggc gttgaaaatt ctattccgtc attgaaagct
     4741 gctgcaaaag cgaatgtcga atacgttgag ttagatacaa ttatgacgaa agataaacaa
     4801 tttgttgtta gtcatgataa caatttgaaa cgtttaacag gtgttaataa aaatatttct
     4861 gaatctaatt tcaaagatat cgtcggtttg aaaatgcgtc aaaatggaca tgaagcaaaa
     4921 tttgtatcct tagacgaatt tattgaaacg gctaaacaat caaatgtgaa gctactagta
     4981 gagttaaagc cacatggtaa agaaccagca gattatacac aacgtgttat tgatattttg
     5041 aaaaagcatg gtgttgaaca tcaatatcgt gtgatgtctt tggattatga tgtgatgact
     5101 aagttgaaaa aagaagcgcc atatctcaag tgtggttata tcattccgtt gcagtttggt
     5161 cattttaaag aaacatcatt agatttcttt gtcatcgaag atttttctta ttcgccaaga
     5221 cttgttaatc aagcgcactt ggaaaataaa gaagtctata cttggactat taacggcgaa
     5281 gaagatttaa cgaaatactt acaaaccaat gttgatggta ttatcacaga tgacccagca
     5341 ttagctgatc agattaaaga agaaaagaaa gacgaaacat acttcgatcg ttctataaga
     5401 attttgtttg aataatataa acaaagacct ctaaagttat caagatgata ccttcagagg
     5461 tctttttaat gttgccatct atgggatagg caatcgtttc attcgtttat attcatatga
     5521 caagtatttg tatggcaatt tggcgtcaca aacacttaca tgatttattg gtgaattatt
     5581 aattgttttg tgaatgcaaa gggttagaaa ttgaattgta aatactttct aatctttgtt
     5641 tcgctttagt catttgatcc aaatttttag tgcgtatagc ggattttgca atatagtgcg
     5701 cagctaaaat atcgcgtttt tgaaacgcat ctaaatttag gtacgataat ttatttaagt
     5761 cagtgtttgc tattaattca tgtaattgat ctacaagcgc ttgatgttga tacgtatgtg
     5821 atgtagtttc agatttgctt gctaatttaa taccagtcgt atcaaggagc gccgctttaa
     5881 taccagcaac taaatatgtt ttgattttca tttgtgttgt catgctttgt tact
//
LOCUS       DAMQYT010000156         5926 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMQYT010000156 DAMQYT010000000
VERSION     DAMQYT010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.158"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            61..663
                     /locus_tag="PGA33_002137"
     CDS             61..663
                     /locus_tag="PGA33_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473938.1"
                     /translation="MLISLVIPVFALLVIGGIIWMIIEGIVHISKKNKAIDNFFNQVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSAFLFWLVILTFGIAGIIFLMPYGLCF
                     LPFYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNITER"
     gene            930..4082
                     /locus_tag="PGA33_002138"
     CDS             930..4082
                     /locus_tag="PGA33_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HDG6473939.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNAERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSEIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQHIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQVYHLDNETLRGNKEIEDLDNRINYIVKQIETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            4139..4621
                     /locus_tag="PGA33_002139"
     CDS             4139..4621
                     /locus_tag="PGA33_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473940.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKR
                     "
     gene            4826..5812
                     /locus_tag="PGA33_002140"
     CDS             4826..5812
                     /locus_tag="PGA33_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HDG6473941.1"
                     /translation="MKKCIKTLFLSIILVVMSGWYHSAHASDSLSKSPENWMSKLDDG
                     KHLTEINIPGSHDSGSFTLKDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYTWPGYDDIVSEIIDSNK"
BASE COUNT         2263 a          722 c         1097 g         1844 t
ORIGIN      
        1 aattagaatg attcaattca atcataattg tttgaaaata gaggtgagca ggtgaacgat
       61 atgttaatta gtcttgtaat cccagttttt gctttgttag ttattggtgg tattatttgg
      121 atgattatag aaggtatagt acatatttca aaaaagaata aagcaattga taactttttt
      181 aaccaagtta ataaagtaag tgagacatat aaattcgcta ctactttttt atttctaatc
      241 ttggctacgg ctggtatttc tcaattttat ctatattata tagtatcagc gtttcttttt
      301 tggcttgtca tacttacctt tggcattgca ggtatcattt ttttaatgcc atatggatta
      361 tgttttctac cgttttataa gcaaaaaaag aaaaaacaga catttaaaaa atacatggtt
      421 tacactacga ttggtttgtc aatttgtcta ggcttatctc tagttttggt tcacactacg
      481 aaaatttata tggacgaagg tggcgtaaga tactattacg gtagttttgt aatgaaacaa
      541 gcgggcggtt atgcttattt agctttagcg gtactttcaa cgttgttaat tgttgcgaaa
      601 aaagctacaa ataaaaataa agaaatcgaa accgtcgaca atacaaatat aacggaaaga
      661 taattaaggg agtgctcatt caggagtgct cttttttgat gtccaaattt agttgcaaat
      721 gaaggcatag aagaaatttt tatagtcatt tattggataa tgaattatgg tcatatcgat
      781 taattttatt agtgaggatt ttacaaacat ttgttgaata aatatggtaa tgataatata
      841 tgaatatttt tgaaaaaaca ctaatatgat taaattattt gttaaaatag cattaagtaa
      901 aaaacaaata gggaacaagg tgggatttca tgagtcaatt gctaaatgat acgttatcgg
      961 cttggttgtt aattgaatct ttaagtccag gagaagtaaa ttttacagcg gaagatatac
     1021 tctcagctga acattttaaa aatggtgcaa agcaagcaca acttcaaagt tttgatgaat
     1081 attttgaaat atggaatgct gaacgcttta ttatatcaga agaaaaatca gagactgggg
     1141 aacttatatt taaattttat agacattgct tccgctataa tgaaattaat ttgaaaattc
     1201 aggatatttt tgatgattat tctgagattc ataatccaaa tgggacacac tgttatggtt
     1261 acacatttaa cacagataaa cacggcaaag tgatagttga ttctatacat attccgatga
     1321 ttatgagtgc attaaaagaa attgaaaaga acaaaaatgc caatatagaa gaaaaattta
     1381 atgattctgt tgaaaaattt gttcaaaaag taaaagaaat tttagcagat gaaccaatta
     1441 atgaatttaa attgaagaag atggacaaag cttatgatga gtacttttct gtattaaatt
     1501 caaagaaaga tggattattt ggacattatg tagcaataga atatgtgaaa gatagtgatt
     1561 taccacagcc ggaatttaac agcttcttca taagtgatat tgagaaagca agaaaatctc
     1621 ccaaccaaac tttaattgat tacattgaag gtgtagaaga aagtcagcac atagaagtag
     1681 atgaaaataa agaaatgttt gacaaatttt tacatccttc acgtttgcct gatggacgat
     1741 ggccatcaca gactgagttt agattgtctt taatgcaaca acttgctgta aaccaaatta
     1801 cgagtggtaa tgaaagaata agttcagtta atgggccacc aggtacaggt aagactactt
     1861 tattaaaaga tatatttgct catctagtag ttgaaagagg taaagagtta gctaaactaa
     1921 ataatcctaa agatgcattt gtcaaaacaa aaattcatga aacggatgat aaatacgtat
     1981 acttactaaa ggaatctatt gccaaatata agatggtagt cgcatctagt aataatggag
     2041 ctgttgaaaa tatatctaaa gatttaccga aaattgaaga aattataaga aatcccgaaa
     2101 aatgtaaatt ccctaaatat gaacagaatt atgcaaattt agcacatgaa ttaaaagatt
     2161 ttgctgaaat agctgaagat ttgattggtg aaagtgcctg gggcttattt tctggagttt
     2221 ttggtaaaag tactaatatt aaccaagtat tgagtcatat gttaaaacaa gatgcgaatg
     2281 atattggctt tgctaaatta ctacaaaatg agaataatcg tatgagttat aacgagttaa
     2341 tgagtgaatg gcaatcacat caacgtgcat ttttagaaga gttgaggcat gttgaaatgt
     2401 taaaagaaga atctattaga gcatatgatg tttataaaaa ttgtgagtct ttctctaaga
     2461 ttgaacaggt tattaatagt gaaaaaacaa gtattgaaga acaggtatat catttagata
     2521 atgaaacgtt acgaggcaat aaagaaatag aagatttgga taatcgaatt aattatattg
     2581 ttaagcaaat agaaacttta aatgagttaa ttaaatccat caaagaaagc aacaaaggtt
     2641 ttattaacaa actgaaagcg atgtttaatt cagaagaaga tgaaagctat aaagatcata
     2701 ataaagagaa gcaacaatta ttaacacaac agttagagtt agagaaatgt aaaaaaaaca
     2761 aacatgaaga ccttgttagc aaactaaaag aaaaagagaa attaattaaa caattaacta
     2821 aagtacagtt gcaattagac gagttaaatt cacagttaca agagttagaa gcatatcgta
     2881 ttgagtcaaa aattacaatt ccagaaaaag atttttggag tgacaacaat tatgatgagc
     2941 gccaagttac taatctgtgg acgagtgacg aacttcaata cagacgtgcc atgctctttt
     3001 taagagcaat gatattgcat aaattattat tgattgctaa taatacaact atttattatg
     3061 cgattaatga ttttaaagat agaaggaaat taattgatgc aaatccagat aaagtacaca
     3121 acgcatggaa tgtgatgcat ttaatatttc cagtagttag tacgacgttt gcaagcttta
     3181 aatctatgta tgggggcata ccaaaagatt tcatagacta cttatttatt gatgaagcag
     3241 gacaagcaat acctcaagca gctgtgggag cattatatcg ttcaaaaaaa gttgtagctg
     3301 taggtgatcc gattcaaata gaaccggttg tgactttaga aagtcattta attgataaca
     3361 ttcgtaaaaa ttatcatgtt ccggaatatc tagtttctaa agaagcttct gtgcagtctg
     3421 ttgcagacaa cgccaatcaa tatggttttt ggaaatctga tgctactgat agtaatcaaa
     3481 aaacctggat aggcatacct ttatgggtgc acagacgatg tttaaaacct atgttcacga
     3541 tagctaacca aatcgcttat aataataaaa tggtgttgcc aagtaatatt acaaaagtag
     3601 gtaaaacagg ttggtatgac gttaaaggaa acgcagttca aaaacaattt gtgaaagagc
     3661 atggtgaaaa agtagtggga ttattagctg atgattggat tgaagcaatt aaggaaggta
     3721 aaaatgaacc gagctcattt gtaatatcgc ctttttcagc agtacagcaa cagattaaac
     3781 gtatgttaaa gcaacaacta ccgactagaa ttgatattga acgtacaaaa attaatcaat
     3841 gggtcgataa atccattggt actgttcata cttttcaagg taaagaggct cagaaggtgt
     3901 attttgtaat aggtactgat aatacccaag atggtgctgt gaactggtca tgcgaaaaac
     3961 caaacttgtt aaacgttgca gtgacaagag ctaagaaaga gttttatgta attggcgaca
     4021 tgcaaagaat acagatgaaa ccattttatg agacgatttt taaagaaaga aatgtaaaat
     4081 aacatacaaa aaagtatata gaggaagtta tacattttaa aaggagcaaa attgaataat
     4141 ggagaaattt aacaactgga tattaaatgc aataagtgga tctcaaacag acaagaatga
     4201 aacaactgaa gaattaaaag gggcaaaatt tatcatttta tatgcatatt caatgctcgt
     4261 tttgcttgcg ttagtaattt ctaacatatt cattcacatt ttggagccta aactatcaat
     4321 caccactcaa atcatcatcg ttttgatttt aattgaagca ctaattggac tgcgtttctt
     4381 gaaagcgtac gatgttaagc gtggcaaaga taaagaaaat aagaaaaata gtaaggattt
     4441 cgttaaacta aaatcaattt tagtagcaat tttatttaca tcattggcgc tgacagcagg
     4501 tactgtagct gatatatacg gtttcactga cttaggaaat actagaagtg atttaatcgt
     4561 ttggagcata ggtggtatta tatttggcct cgtatgttac acaatggaag ataaaagata
     4621 acgataagga gctggcgatt ataaagctag ctcctttttt aacttatata tgtaaagaac
     4681 tatcctaagg gtttttaatc atatgtcaat aatttctata atacattatt aaaacatcaa
     4741 ttaaataagt tttaaaattt tacacatatt tttatttaaa aaagatgtat aattaatgta
     4801 ttaaatatag aaagaagttg atattatgaa aaagtgtatt aagactttgt ttttaagtat
     4861 cattttagta gtgatgagtg gttggtatca ttcagcacat gcgtcagatt cgttgagtaa
     4921 aagtccagaa aattggatga gtaaacttga tgatggaaaa catttaactg agattaatat
     4981 accgggttca catgatagtg gctcattcac tttaaaggat ccagtaaaat cagtttgggc
     5041 aaagactcaa gataaagatt accttaccca aatgaagtcg ggagtcaggt tttttgatat
     5101 tagaggtaga gcaagtgctg ataatatgat ttcagttcat cacggcatgg tttatttgca
     5161 tcatgaatta ggaaaatttc tcgatgatgc taaatattac ttgagtgctt atccaaacga
     5221 aacaattgtg atgtctatga aaaaggacta cgatagcgat tctaaagtta cgaagacatt
     5281 tgaagaaatt tttagagaat attattataa taacccgcaa tatcagaatc ttttttacac
     5341 aggaagtaat gcgaatccta ctttaaaaga aacgaaaggt aaaattgtcc tattcaatag
     5401 aatggggggt acgtacataa aaagtggtta tggtgctgac acgtcaggta ttcaatgggc
     5461 agacaatgcg acatttgaaa cgaaaattaa taatggtagc ttaaatttaa aagtacaaga
     5521 tgagtataaa gattactatg ataaaaaagt tgaagctgtt aaaaatttat tggctaaagc
     5581 taaaacggat agtaacaaag acaatgtata tgtgaatttc ttgagtgtag cgtctggagg
     5641 cagcgcattt aatagtactt ataactatgc atcacatata aatcctgaaa ttgcaaaaac
     5701 gattaaagca aatgggaaag ctagaacggg ttggctgatt gttgactatg caggatatac
     5761 gtggcctgga tatgatgata tcgtaagtga aattatagat agtaataaat aaggattcaa
     5821 taatgatatt aagacgagta tgaaaatagt tagattctaa ttattttcac tactcgtttt
     5881 tattttgaaa ataagtaata attcaacaat attataaatt gaacag
//
LOCUS       DAMQYT010000157         5879 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMQYT010000157 DAMQYT010000000
VERSION     DAMQYT010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.159"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            36..743
                     /gene="splA"
                     /locus_tag="PGA33_002141"
     CDS             36..743
                     /gene="splA"
                     /locus_tag="PGA33_002141"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplA"
                     /protein_id="HDG6473942.1"
                     /translation="MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITD
                     ATKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDV
                     KDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTK
                     YKMFESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFG
                     VYFTPQLKEFIQNNIEK"
     gene            868..1590
                     /gene="splB"
                     /locus_tag="PGA33_002142"
     CDS             868..1590
                     /gene="splB"
                     /locus_tag="PGA33_002142"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HDG6473943.1"
                     /translation="MNKNVVIKSLAALTILTSVTGIGTTLVEEVQQTAKAENNVTKVK
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            1648..2367
                     /gene="splC"
                     /locus_tag="PGA33_002143"
     CDS             1648..2367
                     /gene="splC"
                     /locus_tag="PGA33_002143"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HDG6473944.1"
                     /translation="MNKNIVIKSMAALAILTSVTGINAAVVEETQQIANAEKNVTQVK
                     DTNIFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQAFNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            2488..3207
                     /gene="splD"
                     /locus_tag="PGA33_002144"
     CDS             2488..3207
                     /gene="splD"
                     /locus_tag="PGA33_002144"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplD"
                     /protein_id="HDG6473945.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTVVDGIQQTAKAENSVKLIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVTSDAVVQPGSSGSPILNSKREAIGVMYASDKPTGEST
                     RSFAVYFSPEIKKFIADNLDK"
     gene            complement(3156..3263)
                     /locus_tag="PGA33_002145"
     CDS             complement(3156..3263)
                     /locus_tag="PGA33_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473946.1"
                     /translation="MLILLLISKNHLSMYGWMIYLSKLSAINFLISGEK"
     gene            3365..4081
                     /gene="splE"
                     /locus_tag="PGA33_002146"
     CDS             3365..4081
                     /gene="splE"
                     /locus_tag="PGA33_002146"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplE"
                     /protein_id="HDG6473947.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIK
                     NTNVAPYNGVVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKV
                     KKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
                     NKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTK
                     GYGVYFSPEIKKFIADNTDK"
     gene            4232..4951
                     /gene="splF"
                     /locus_tag="PGA33_002147"
     CDS             4232..4951
                     /gene="splF"
                     /locus_tag="PGA33_002147"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HDG6473948.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTMVEGVQQTAKAENTVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGEST
                     RGFAVYFSPEIKKFIADNLDK"
     gene            5016..5150
                     /locus_tag="PGA33_002148"
     CDS             5016..5150
                     /locus_tag="PGA33_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473949.1"
                     /translation="MHYLICIKANQRLIFTNSIVDWFYLCMNEQLFDIIKNINDFESI
                     "
     gene            5314..>5879
                     /locus_tag="PGA33_002149"
     CDS             5314..>5879
                     /locus_tag="PGA33_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HDG6473950.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFL"
BASE COUNT         2286 a          863 c         1045 g         1685 t
ORIGIN      
        1 ttaactataa aaataaattt ggaaggaggt aaaatatgaa taaaaatgta atggttaaag
       61 gtttaactgc tttaactatt ttaacatctc ttggttttgc tgaaaatatt tctaatcagc
      121 ctcattcaat tgccaaagca gaaaagaatg tcaaagaaat taccgatgca actaaggaac
      181 catacaattc agtggtagca tttgtgggtg gtactggtgt agttgttggt aaaaatacaa
      241 tcgtaactaa caaacatatc gctaaaagta atgatatttt taaaaataga gtatcagcac
      301 atcattcgag taaaggtaaa ggcggaggaa actacgacgt taaagacatt gtagaatatc
      361 ccggaaaaga agaccttgcg atagttcatg ttcatgaaac aagtacagaa ggtttgaatt
      421 ttaataagaa cgttagttat acaaaatttg cagacggagc aaaagtgaaa gatagaattt
      481 ctgttattgg ttatccaaag ggtgcacaaa caaaatataa aatgtttgaa tcgacaggaa
      541 cgattaacca tatcagtgga acgtttatgg aatttgatgc gtatgcacaa ccaggtaatt
      601 caggatctcc tgtattgaat tctaaacatg aactgattgg tattttatat gcaggtagtg
      661 gaaaagatga atctgaaaag aatttcggtg tttatttcac accacaatta aaagaattta
      721 ttcaaaataa tattgaaaaa taagtatcat caattcattc gtgaagttga ttttttaaag
      781 agtgattaag aaaacggtta cataattaac taaatatact gaattatgta tctagatatc
      841 aaaataatta aaagagagga acttaaaatg aacaaaaacg tagtcatcaa gagtttagca
      901 gcattaacaa ttttaacatc tgtaacaggt attggaacaa cattggttga ggaagtacaa
      961 caaactgcca aagcagaaaa taatgtcaca aaagttaaag atactaatat ttttccatat
     1021 actggtgtag ttgcttttaa aagtgcaact ggatttgtag ttggaaagaa tactatttta
     1081 acaaataaac atgtgtcgaa aaattacaaa gtgggcgatc gtattactgc acatccaaat
     1141 agtgataaag gtaatggtgg tatttattcg attaaaaaga ttattaatta tccaggtaaa
     1201 gaagatgtat cagtcattca agttgaagag cgtgcaatag aacgtggacc aaaaggcttt
     1261 aattttaatg ataatgtaac gccattcaaa tatgcggcag gggctaaagc tggtgagcga
     1321 attaaagtga ttggttatcc acacccatac aaaaataaat atgttttata tgagtcaact
     1381 ggccctgtga tgtcagtaga aggtagcagt attgtatatt cagcgcatac tgaaagcgga
     1441 aactctggat cacctgtatt aaacagcaac aacgaattag ttggtattca ttttgcttct
     1501 gatgtaaaaa atgatgataa cagaaatgca tatggcgtct actttacacc agaaattaaa
     1561 aaattcattg cagaaaacat agataaataa acaaattgac tttaaacgag cgttgcaaca
     1621 tatctcgaat tgtaaaggag cttgaaaatg aataaaaata tagtcattaa aagcatggca
     1681 gcattagcca ttctaacctc agtaactgga ataaatgctg cagtcgttga agagacacaa
     1741 caaatagcaa atgcagagaa gaatgttacg caagttaaag atacaaatat ttttccatat
     1801 aatggcgtcg tttcatttaa agatgcgaca ggttttgtaa ttggaaaaaa tacaattatc
     1861 accaataaac atgtatcaaa agattataaa gttggcgata gaattactgc ccatccaaac
     1921 ggtgacaaag gaaatggtgg tatatataaa attaaaagca tttctgatta tccgggtgat
     1981 gaagacatct ctgtcatgaa tattgaagaa caagcagtcg aacgtggacc aaaaggcttt
     2041 aattttaatg aaaatgtcca agcattcaat tttgcgaaag atgctaaagt tgatgacaaa
     2101 attaaagtta ttggttaccc attacctgct caaaatagtt ttaaacagtt tgaatctaca
     2161 ggaactataa aaagaatcaa agacaatatt ttaaattttg atgcatacat tgaacccggg
     2221 aattcaggat caccagttct aaattctaac aatgaggtca taggtgtggt gtatggcggt
     2281 attggaaaaa ttggttctga atataatggt gccgtatact ttacgcctca aatcaaagat
     2341 tttattcaaa agcacattga acaataaaca aatttaaata tacaccatga gcatgtgttc
     2401 aataatttta atgaaaaaca tcggtcgaat ataacataaa aaaacgtcta tatcaaaagc
     2461 atcatgaata aacagaggag cacaaaaatg aataaaaata taatcatcaa aagtattgcg
     2521 gcattgacga ttttaacatc aataactggt gtcggcacaa cagtggttga tggtattcaa
     2581 caaacagcca aagcagaaaa tagtgtgaaa ttaattacca acacgaatgt tgcaccatac
     2641 agtggtgtta catggatggg cgctggaaca ggatttgtag ttgggaatca tacaatcatt
     2701 accaataaac atgttactta tcacatgaaa gtcggtgatg aaatcaaagc acatcctaat
     2761 ggtttttata ataacggtgg tggactttat aaagttacta agattgtaga ttatcctggt
     2821 aaagaagata ttgcggtcgt acaagttgaa gaaaaatcaa cgcaaccaaa aggtagaaaa
     2881 ttcaaagatt tcactagcaa atttaatata gcatcagaag ctaaagaaaa tgaacctata
     2941 tcagtcattg gttatccaaa tcctaatgga aataaactac aaatgtatga atcaactggt
     3001 aaagtactat cagtgaatgg aaatatagtg acatctgatg cggttgtcca acctggcagc
     3061 tctggttcac ctatattaaa tagtaagcga gaagcaattg gtgttatgta tgctagtgat
     3121 aaaccaacag gtgaaagtac aaggtcattt gctgtttatt tctctcctga aattaagaaa
     3181 tttattgcag ataatttaga taaataaatc atccatccat acattgataa atgattttta
     3241 gaaattaaca acaaaatcaa caattttaaa catctctgtg attctattta ttcgaaatga
     3301 tttaaaaaat aaaacttcaa aaacctaacc ttatatttat acgaatactt agaggagcac
     3361 aaaaatgaat aaaaatataa tcatcaaaag tattgcagca ttgacgattt taacatcagt
     3421 gactggcgtc ggcacaacag tggttgaggg tattcaacaa acggctaaag ctgaacataa
     3481 tgtgaaacta atcaaaaata ctaatgtagc accatacaat ggtgtcgttt cgataggatc
     3541 tggaacaggt ttcattgtcg gtaaaaatac aattgttacc aacaagcatg tcgttgcagg
     3601 tatggaaatt ggtgcacata ttatagcgca tcccaatggt gaatataata atggcggatt
     3661 ttataaagtt aaaaaaattg tccgttattc aggtcaagaa gatattgcca ttctacatgt
     3721 ggaagataaa gctgttcatc caaaaaacag gaattttaaa gattacacag gcattttaaa
     3781 aatagcatca gaagctaaag aaaatgaacg catttcaatt gttggctatc cagaaccata
     3841 tataaataaa tttcaaatgt atgagtcaac aggaaaagtg ctgtcagtta aaggcaacat
     3901 gattattact gatgctttcg tagaaccagg caactcaggt tcagctgtat ttaacagtaa
     3961 atacgaagtt gtaggtgttc actttggtgg aaacggccct ggaaataaaa gtacaaaagg
     4021 atatggtgtt tatttctctc ctgaaattaa gaaattcatt gcagataaca cagataaata
     4081 aatccttaca tagataaatg attttaaaaa ttaacaacaa actcaacaat tcaaatcatc
     4141 tctgtgattc catttattcg aaatgattaa aaaaaataaa acttcaaaaa gctaacatta
     4201 taattataca aatacttaga ggagcagaaa aatgaataaa aatataatca tcaaaagtat
     4261 tgcagcattg acgattttaa catcaataac tggtgtcggc acaacaatgg ttgaaggtgt
     4321 tcaacaaaca gccaaagccg aaaatactgt taaacaaatt acaaatacaa atgttgcacc
     4381 atacagtggt gttacatgga tgggcgctgg aacaggattt gtagttggaa atcatacaat
     4441 cattaccaat aaacatgtta cctatcacat gaaagtcggt gatgaaatca aagcacatcc
     4501 taatggtttt tataataacg gtggtggact ttataaagtt actaagattg tagattatcc
     4561 tggtaaagaa gatattgcgg ttgtacaagt tgaagaaaaa tcaacacaac caaaaggtag
     4621 aaaattcaaa gatttcacta gtaaatttaa tatagcatca gaagctaaag aaaatgaacc
     4681 tatatcagtc attggttatc caaatcctaa tggaaataaa ctacaaatgt atgaatcaac
     4741 tggtaaagta ttatcagtga atgggaatat agtgtcatcg gatgcaatta ttcagcctgg
     4801 tagctctggt tcacctatat taaatagtaa acacgaagct attggtgtaa tctatgccgg
     4861 taataagcca tcaggtgaaa gcacaagagg atttgctgtt tatttctctc ctgaaattaa
     4921 gaaattcatt gcagataatt tagataaata attaaaactt agacattcac ccaatcctga
     4981 caaaatatac tataactaac atttattaat atatattgca ttatttaata tgcatcaaag
     5041 ccaatcaacg attgattttc accaactcaa ttgttgattg gttttattta tgtatgaatg
     5101 aacaactttt tgacatcatt aagaatataa atgattttga aagcatttga aagctacaac
     5161 atttctataa aatttttcaa taacaattgc gccactaaaa ctcaaaattt ccaccaccaa
     5221 catccaaatt atcaacatcg caacataacc aaatgttata ataaatctat tacacaaaga
     5281 gataaattac ttatgcaaag gcggaggaat cacatgtcta ttactgaaaa acaacgtcag
     5341 caacaagctg aattacataa aaaattatgg tcgattgcga atgatttaag agggaacatg
     5401 gatgcgagtg aattccgtaa ttacatttta ggcttgattt tctatcgctt cttatctgaa
     5461 aaagccgaac aagaatatgc agatgccttg tcaggtgaag acatcacgta tcaagaagca
     5521 tgggcagatg aagaatatcg tgaagactta aaagcagaat taattgatca agtcggttac
     5581 ttcattgagc cacaagattt attcagtgcg atgattcgtg aaattgaaac gcaagatttc
     5641 gatatagaac atctagcgac ggcgattcgc aaagttgaaa catctacatt aggtgaagaa
     5701 agtgaaaatg actttatcgg gctgttcagc gatatggatt tgagttcaac gcgactaggt
     5761 aacaatgtca aagaacgtac tgctttaatc tctaaagtca tggttaatct tgacgactta
     5821 ccattcgttc acagtgacat ggaaattgat atgttaggtg atgcatatga attcctaat
//
LOCUS       DAMQYT010000158         5804 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMQYT010000158 DAMQYT010000000
VERSION     DAMQYT010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.160"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            123..1061
                     /locus_tag="PGA33_002150"
     CDS             123..1061
                     /locus_tag="PGA33_002150"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDG6473951.1"
                     /translation="MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSEELLPQHSQLNTNKTKKKNMANSFLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAALHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL"
     gene            complement(1232..3634)
                     /locus_tag="PGA33_002151"
     CDS             complement(1232..3634)
                     /locus_tag="PGA33_002151"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDG6473952.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSEAQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELDLSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQDKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPILAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(3634..4692)
                     /gene="pheS"
                     /locus_tag="PGA33_002152"
     CDS             complement(3634..4692)
                     /gene="pheS"
                     /locus_tag="PGA33_002152"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDG6473953.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEDKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(5073..5804)
                     /locus_tag="PGA33_002153"
     CDS             complement(5073..5804)
                     /locus_tag="PGA33_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDG6473954.1"
                     /translation="MTSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQSGIV
                     ITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDISKAQ
                     QVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLPVMT
                     QDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQNLI
                     IPIYGKAESLNVAIAGSILLYHLKG"
BASE COUNT         1852 a         1137 c          839 g         1976 t
ORIGIN      
        1 tattttgtaa gtatccgcaa ttaattcttt acaacataca taaatgtttc tgacgttatt
       61 atcatttatg atatgattat ttttgctaaa gacaatgaaa ttttatgaaa ggatttacac
      121 aaatggcgaa tatcgttttt aaattgtcgg ataaagacat aacgacatta atgtcacgca
      181 tttcttttga tactgagaat ttacctcaag gtatgaaagc acgtgcaaag tatcaaaata
      241 caactgtaaa tatttaccaa tcaggtaaag tcatgtttca aggtaatcat gcagaagctg
      301 tgtctgaaga acttctaccg cagcattctc aattgaatac taataaaact aaaaagaaaa
      361 atatggctaa ttcttttctt gaacaaactt taatgtatga tcaatttaac tgtattggta
      421 gtgatgaggc tggaagtggc gattactttg gtccgttgac tgtgtgtgct gctttcgtta
      481 ccaaagagca tgtccctatt ttaaaaactt tgggtgtaga tgattcaaaa aaattaaccg
      541 ataccaaaat tgtagaactt gctgagcaat tggttacttt cataccacat tcattattaa
      601 cactacacaa tgaaaaatat aatattcagc aagcaaaagg ctggactcaa gtaaaaatga
      661 aagctgccct tcataatgaa gccatcaaaa atgtacttga aaaaattgat tcgtctcaat
      721 tagactatat cgtcattgat cagttcgcaa aacgcgaagt gtatagccat tacgcattat
      781 cagatatacc acttccaaag aaaacaaaat ttgaaacaaa aggagaatcg aagtcattag
      841 ccattgcagt ggcaagcata atttctcgat acgcatttgt tacatatatg gatcaaatat
      901 caaaaaatat taatatgacg attcctaaag gtgccggtgc taaagtcgat gtaatcgccg
      961 ccaaaattat aaaaaaatac ggtctttcac gtttagatac catttcaaaa aagcatttta
     1021 aaaatcgtga aaaagcccaa aaaattttaa agcctctttg atttttaatc attagaggct
     1081 tttatgcagt tgtgcgaagc atcatctgta ttccgctgtt cctctttgac tttttaaatg
     1141 cattggaacc aaattggtga acccaaaaat aaaaccccat gttagggata tcaatctact
     1201 tcagatatcc ttatacatgg ggtttaaatc attatctaat aacagcacct tgttcaatta
     1261 atgctgcttc aatttccgct tgtacttttg aaacgcgctc atctgtcaat gtttcttctg
     1321 tgtctaaata atttaaacgt attgcaattg attttttacc tttttctaaa tgttcgccct
     1381 gatatacatc aaagacaagt gtatctttta atatattgcc accatgtgca tgaatcgttg
     1441 ataataaatc agctgctgga atattttgat ctacttctaa tgcaatgtca cgagacatgc
     1501 ctgggaatct cggaattggc tggtaattaa tgtaacctac cgacacagcc attaatgcat
     1561 caaaattcaa ctcaaaaaca tacgtacgtt ttaaatcatt atcagctgct aatattggat
     1621 gtaattcacc aataaaacca acaactttat tctctaataa gatttcagca gtacgacctg
     1681 gatgtaatcc atcaatatca gcacggcgat aactaaattc aagatttaac ttttcagata
     1741 ctcgatccac gacacctttt gctaaataga aatcaaccgt ttctttctta tcttgccatt
     1801 gattgactac ataatctcca gttaaaatac cacttaaata ttcaacttga tctggtagtt
     1861 caccttctcc attagcaaag aagacattgc cgatttcaaa taattttaca tctttatttt
     1921 tgcgtgccac attatatgat gccgcttcga ttaaatgtgg taataaactt tgacgtaatg
     1981 acgcatgcgc ttcactcatt ggcatcaata aatcaattgt ttgacgctgt tgcatcgaaa
     2041 atgcagtagc atcttcttta gaaactaacg aatacgtaat agcttggtct aatccagcac
     2101 cttctaacac ttctttaacc attctagttt tatattggcg atcagttagc tgaccactag
     2161 taactttatc gaagacaggt aacgttgatg gaatatcgtc gtatccatat atacgtgcaa
     2221 cttcttcaat taaatcttct ttaattgtaa tatctttacg acgtgatggt actagcactg
     2281 taataacatc atcatttatt tctgtatcaa accctagttg attaaaaata gtaacaatat
     2341 cattttgtga caaatcaaat ccaatagtgc gattaatttt atcagcagtg atgtcgattg
     2401 gtgtaataaa tgcaccaagt tctcctgaag acactctatc ttttagcact tttccgtttg
     2461 cataagtttg taataaataa catgcacggt cgactgcttc atctacaaat tcagtagcta
     2521 ttcctttttc aaaacgacta gatgattcac tgcgtaaatt taaacgtctt gatgtatgac
     2581 gaattgaaac tggatcaaaa atagcacctt caatcactat atttgatgtt tgttctttaa
     2641 cttctgaaaa atcgccaccc ataacaccag ctaatgcaat tggagtttgt ccattagtaa
     2701 tgacaatatc gctcgttaat aattcacgtt ctgtatcatc taatgttgtc attttttcgc
     2761 cttcattagc ttgacgaaca acaatttgtt gtgaaccaat cgcatcttga tcaaacatgt
     2821 gcaatggttg accgtattct aataacacat aatttgaaat gtcaacaaca ttattaatag
     2881 gacgtatacc cgcttttatt aagcgtgctt gcatccaaat tggcgagggt tcaattgtca
     2941 cgtcgtgaac aacacgtgca ctataatatg gtactttatc ttcattttca attgtcacag
     3001 tcagttcatc atttgcagat aaatcaagct catttgatgt tgtctctggc ttagtcattt
     3061 ttgtattata taatgctgca acttcataag cagtacctat catacttaaa gcatctgcac
     3121 gattcggcgt taaatcaaat tccattactt gatcatctaa atataaagct tgtaaggcat
     3181 ctgttcctgg aacttgggct tcactaaata cataaatgcc tgattcaaaa cttttcggta
     3241 tatagttact tgaaatacca atttcttgta acgaacaaat cataccttct gaacgttcac
     3301 cgcgtaattt ggcacgctta attttaatac caccaggcaa tctgccacct actttagcaa
     3361 caatgacata ttgtcctgca tcaacgttcg gtgcaccaca aacgatttgt acaggttcgt
     3421 cttctccgat atcaacttgg caaacattta atttatcagc atcaggatgt ttctcttttg
     3481 acttaacgaa gccgacaact aaatttttga tatcttttgt gtagtcaatt aaatcatcca
     3541 cttcaatacc tgtgcgcgta atacgttctg ccaaattact tacagaatca tcgattgtta
     3601 catattcttt caaccattca tttgatatca acattacatg tcacctctat cttctaccgc
     3661 tttaaattga tctaaaaatc tcacatcatt agtatagaaa tgacgaatat cttctatacc
     3721 atatttcaac attgcaatac ggtctggtcc cataccaaat gcaaatccag agtactctga
     3781 agaatcaaaa ccagccattt ctaatacatt aggatgtacc attccagcac ctaaaatttc
     3841 aatccatcct gtgtgtttac acacattaca accttttcct ttacatttaa aacatgacac
     3901 atcaacttct acagaaggtt cagtgaatgg gaagtaactt ggacgtaaac gaatttcacg
     3961 atcagcacca aataatttct tagctaacaa ttctaaagta cctttcaaat cactcatttt
     4021 aacgttttta tcaacaacta atccttcgat ttgtgtaaat tgatgactat gtgtcgcatc
     4081 atcagagtca cgacgataca ctttaccagg gcaaataatt ttaactggac cttgaccatg
     4141 acgtgattcc atcgtacgtg cctgcactgg tgatgtatgc gtacgtaata aaatttcatc
     4201 cgtaatatag aaactatctt gcatatcacg tgcagggtgt gatttaggta aattcagcat
     4261 ttcgaagtta taatgatctt gttcaacttc atatccattc acaatttcat aacctaaacc
     4321 taagaataag tcttcaattt cttctattgt acgtgttaat ggatgctttg aaccgatttc
     4381 aatatgacga cctggtaatg atacatcaat tgtttcttca gccaattgct tatttaattt
     4441 ttctttaact aacatctgtt gtctttcatc taattcattt tgaattgttt gacgcaattc
     4501 attcactttt tgaccaaacg caggtttatc ttcattcggc aaatccttca tcaatttcat
     4561 tagtccgcta actgaccctt ttttacctaa gtatttcact ttaacttctt gcagtgcacg
     4621 ttcatcattt gcttcattaa tatctacaag cgcttgttgt tttaactctg acattgtttg
     4681 ttgttcagac atatgtcact gacctcccta ttcgaactat tcattttcta tttatcatat
     4741 ttgttgttta actaaaaaaa gactttcatc ctcttaaaaa ataagggacg aaagtcattt
     4801 ccgtggtacc acccaaattt atgcctaagc atacacttgt ttaatattga taacggattg
     4861 tctttccgac ttaaatctct ttaagtaacc aaaaaggtga aaagacttta aataattgga
     4921 ataacttgca gtcacgatta ttctccctaa aacaattatt tatataaatg tccatgcctt
     4981 tttattggtt tatattcaat tgtgtatgca ttcacgtaga aatgttctgt taaacaatta
     5041 attataatta tatcggaaaa ctttcaacac ggtcaacctt tcaaatgata aagtaaaata
     5101 ctacctgcaa tcgctacatt taaactttca gctttaccat aaataggtat gattaaattt
     5161 tgtgtagtat gtgctaataa ttcaggatta acaccttctc cctcattacc taatagtaat
     5221 gcaaaagaat cacttgaagt aacttctttg tatgccactg cgttttcaag tgctgtacca
     5281 taaacaggac cattaaattg agtaataaac gtatcgagat cttgtgtcat aactggcaaa
     5341 tggaaaacac taccttgact cgctcgcaac actttatctt gataaggatc tgtcgtaccc
     5401 ttctccatta ttacagcatc cattccagca gcatccgcag ttctaattaa tgtgcctaaa
     5461 tttccaggat cttgaacacg atcgatgagc aatacttgtt gtgctttaga aatatcataa
     5521 tgcggcttct caatgattgc gaaaaaccct tgtggtgtca ctgtacctga taaagattca
     5581 gcaactttca tgtttatttc aaaaacttct tgcgcgtatg cgataatttg ctgatctaat
     5641 cttgccggtt caattgcaaa taattgtgta attacaattc cactttgata agcttcttca
     5701 attaaatgca caccttcaat taaagctaat ccagttttat ccctctcacg tttctttttt
     5761 agcttgttcg cttgtttaat tctattattt tgtgcagaag taat
//
LOCUS       DAMQYT010000159         5790 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMQYT010000159 DAMQYT010000000
VERSION     DAMQYT010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.161"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            173..1471
                     /locus_tag="PGA33_002154"
     CDS             173..1471
                     /locus_tag="PGA33_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDG6473955.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            1732..2673
                     /gene="pde2"
                     /locus_tag="PGA33_002155"
     CDS             1732..2673
                     /gene="pde2"
                     /locus_tag="PGA33_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HDG6473956.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGNEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVDSWDEFEQLATALRTKLN"
     gene            2694..>5790
                     /locus_tag="PGA33_002156"
     CDS             2694..>5790
                     /locus_tag="PGA33_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDG6473957.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKKVGDQHRDIVQVFETHNDTYMDHLSISIQGRKHVWIQN
                     VCYQTRQDADTISALAAIRDNTKLDLIHDQEDFGAHFLTEKEINQLDINQEYLTQVDV
                     IAQKCDAELKYHQSLLPQYETPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNYKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DE"
BASE COUNT         2097 a          785 c         1100 g         1808 t
ORIGIN      
        1 ataataatgg atttttccat ttaaaattaa agattttgtg atctttgtgt ttgttgtata
       61 ttttcaaaaa ataaaatagg tgaaacgatc attatttcat ttcacttcaa tgctttttat
      121 ccaaaataaa gttataatgt agttaatact gttcgaaaag aagaggtaaa gaatgacaaa
      181 acatgaacaa attttagatt atattgaatc gttatctata ggctctaaaa tttcggtgcg
      241 taaaatcgct aaatttttaa acgtatctga agggacagca tatcgtgcaa ttaaagatgc
      301 tgataaaatg ggcatggtcg caacgataga tcgtgttggt acggttcgaa tcgaaaaacg
      361 taatcgtaat gaaattgaac atttaacttt taatgaaatt gtcaatatta ttgatggcca
      421 agtgttaggt ggcaacaaag gtatcactaa gatggtatct aaatttgcaa ttggagcaat
      481 ggaactcaaa gatattttaa agtatattgg tccaaagaca ttattgattg tcggaaatcg
      541 tgaagatgtg cagattgaag ctttaaaaag aggtacggct atattaatta ctggtggatt
      601 taagccatct aataaagtta ttaattttgc gaatgaacat gatttacctg ttctatcatc
      661 aagttatgat acattcctag ttgcaaatat tatcaacaaa gcacttttta atcagaaaat
      721 acgaaaagat atcttaatag ttcaagatat aatgacgcca ttagatgatt tatctgtgct
      781 gtttgatacg atgaaaatag cagattataa gcgtatggcg aatcgaactg gccatacaag
      841 atttccggta gtcaatgagt cgtataaatt agtaggtatt gtaacaagtc gagagatgat
      901 taatacaaag gacgacgacg aaatagataa agtgatgacg cgaaatccta tttatgtaaa
      961 tgctatgagt acagtagcaa gttgtgcgca tatgatgatt tgggaaggta ttgaattgat
     1021 tcctgtcgtt tcgagtaata aaaagacagt gggtgtgatt aatagacaag atgttttaaa
     1081 gtctatgcaa cttttaggaa gacagcctca gatgggtgaa acaattaacg atcaaatcgc
     1141 caaatatata acaatgaatc aagatggaat tacagtagaa gtttcaccac ttttaatcaa
     1201 tcattacgga acagtaagta aagcggcatt tgtaagtatt atagaagaaa caatacaata
     1261 tgaaatgcgt aaatttaaaa aaggtaatgt catgatagag aacttaaata tcgtgtatat
     1321 taaaacagta cctattgaat cacatataac agtgcgcttt ggcatactag acgtcggaag
     1381 aaacttcgcc aaaatagaag tgaacatgca tagtcaaaac gacaaagtag cgagcgctct
     1441 cgtgatatgc caaatgtttg acgaagtata ggaacttagc agagaaaata ggcataagat
     1501 agatttcgca caaacgttgg ggccccaccc caacttgcat tgtttgtaga aattaggggt
     1561 ccaatttctc tgtgttgggg cccctgaact aaatagtatg aaaatatata tatctaaata
     1621 attaaaacgg gcaaatagtt taatatggta gtaacaattt ttataattta gatattttga
     1681 tatagactaa tgcttaaatt aagcaacttt tctaaaaaag ggagtatgaa aatgattagt
     1741 actttgaatg aaattatgaa atgtatcgaa gacaacgata caattattat acacagacat
     1801 gttagaccag atcctgatgc atatggttca caacttggtt taaaatacta tattcagcag
     1861 aaatttccgc aaaagcaagt atttgcagtg ggtgaagcgg aatcatcatt aagttttatt
     1921 ggagagttag ataacattga tgataaaaca tatcaagatg cgcttgtaat tgtatgtgat
     1981 actgccaatg ctccacgaat tgacgatgaa cgttatagta caggtcgtaa acttattaaa
     2041 atagatcatc atcctgcagt tgatcagtat ggtgatatta atttagttaa tacgaacgcg
     2101 tcatctacaa gtgaaatcat ttacgattta atctcacatt ttaatgatga agcaattgtt
     2161 aataaagata tcgcgagcgt tttatatctt ggtatcgtcg gtgatactgg gcgattcctt
     2221 tttaacaata cctcagaaca tactatggaa attgctggaa agttaattgg gcatgatatt
     2281 gatcataatg cgcttttaaa taaaatgatg gagaaggacc caaaaatgtt gccgtttcaa
     2341 ggttatgttt tacaacattt cgaacttatg gatgatggat tctgccaagt taaaataact
     2401 gaagatgtat tggagcaatt cggtattcag ccaaatgaag catctcagtt tgttaataca
     2461 attgctgaca tcaaaggctt gaaaatatgg gtatttgcag tcgatgaagg taatgaaatc
     2521 agatgtcgat tacgttctaa agggcaattg attattaatg atattgcgca agattttggt
     2581 ggcggtggtc atccgaatgc gtcaggagtt tcagtggaca gctgggatga atttgagcaa
     2641 cttgctacag ctttacgcac aaaacttaac taatagaaag gggccattca atcatggtgg
     2701 catatttaaa tattcatacg gcttatgatt tgttaaattc aagcttaaaa atagaagatg
     2761 ccgtaagact tgctgtgtct gaaaatgttg atgcacttgc cataactgac accaatgtat
     2821 tgtatggttt tcctaaattt tatgatgcat gtatagcaaa taacattaaa ccgatttttg
     2881 gtatgacaat atatgtgaca aatggattaa atacagtcga aacagttgtt ctagctaaaa
     2941 ataatgatgg attaaaagat ttgtatcaac tatcatcgga aataaaaatg aatgcattag
     3001 aacatgtgtc gtttgaatta ttaaaacgat tttctaacaa tatgattatc atttttaaaa
     3061 aagtcggtga tcaacatcgt gatattgtac aagtgtttga aacccataat gacacatata
     3121 tggaccacct tagtatttcg attcaaggta gaaaacatgt ttggattcaa aatgtttgtt
     3181 accaaacacg tcaagatgcc gatacgattt ctgcattagc agctattaga gacaatacaa
     3241 aattagactt aattcatgat caagaagatt ttggtgcaca ttttttaact gaaaaggaaa
     3301 ttaatcaatt agatattaac caagaatatt taacgcaggt tgatgttata gctcaaaagt
     3361 gtgatgcaga attaaaatat catcaatctc tacttcctca atatgagaca cctaatgatg
     3421 aatcagctaa aaaatatttg tggcgtgtct tagttacaca attgaaaaaa ttagaactta
     3481 attatgacgt ctatttagag cgattgaaat atgagtataa agttattact aatatgggtt
     3541 ttgaagatta tttcttaata gtaagtgatt taatccatta tgcgaaaacg aatgatgtga
     3601 tggtaggtcc tggtcgtggt tcttcagctg gctcactggt cagttattta ttgggaatta
     3661 caacgattga tcctattaaa ttcaatctat tatttgaacg ttttttaaac ccagaacgtg
     3721 taacaatgcc tgatattgat attgactttg aagatacacg ccgagaaagg gtcattcagt
     3781 acgtccaaga aaaatatggc gagctacatg tatctggaat tgtgactttc ggtcatctgc
     3841 ttgcaagagc agttgctaga gatgttggaa gaattatggg gtttgatgaa gttacattaa
     3901 atgaaatttc aagtttaatc ccacataaat taggaattac acttgatgaa gcatatcaaa
     3961 ttgacgattt taaaaagttt gtacatcgaa accatcgaca tgaacgctgg ttcagtattt
     4021 gtaaaaagtt agaaggttta ccaagacata catctacaca tgcggcagga attattatta
     4081 atgaccatcc attatatgaa tatgcccctt taacgaaagg ggatacagga ttattaacgc
     4141 aatggacaat gactgaagcc gaacgtattg ggttattaaa aatagatttt ctagggttga
     4201 gaaacttatc gattattcat caaatcttaa cacaagtcaa aaaagattta ggtattaata
     4261 ttgatatcga aaagattccg tttgatgatc aaaaagtgtt tgaattgttg tcgcaaggag
     4321 atacgactgg catattccaa ttagagtctg acggtgtaag aagtgtatta aaaaaattaa
     4381 agccggaaca ctttgaagat attgttgctg taacttcttt gtatagacca ggtccaatgg
     4441 aagaaattcc aacttacatt acaagaagac atgatccaag caaagttcaa tatttacatc
     4501 cgcatttaga acctatatta aaaaatactt acggtgttat tatttatcaa gagcaaatta
     4561 tgcaaatagc gagcacattt gcaaacttca gttatggtga agcggatatt ttaagaagag
     4621 caatgagtaa aaaaaataga gctgttcttg aaagtgagcg tcaacatttt atagaaggtg
     4681 caaagcaaaa tggttatcac gaagacatta gtaagcaaat atttgatttg attctgaaat
     4741 ttgctgatta tggttttcct agagcacatg ctgtcagcta ttctaaaatt gcatacatta
     4801 tgagcttttt aaaagtccat tatccaaatt atttttacgc aaatatttta agtaatgtta
     4861 ttggaagtga gaagaaaact gctcaaatga tagaagaagc aaaaaaacaa ggtatcacta
     4921 tattgccacc gaacattaac gaaagtcatt ggttttataa accttcccaa gaaggcattt
     4981 atttatcaat tggtacaatt aaaggtgttg gttatcaaag tgtgaaagtg attgttgatg
     5041 aacgttatca gaacggcaaa tttaaagatt tctttgattt tgctagacgt ataccgaaga
     5101 gagtcaaaac gagaaagtta cttgaagcac tgattttagt gggagcgttt gatgcttttg
     5161 gtaaaacacg ttcaacgttg ttgcaagcta ttgatcaagt gttggatggc gatttaaaca
     5221 ttgaacaaga tggtttttta tttgatattt taacgccaaa acagatgtat gaagataaag
     5281 aagaattgcc tgatgcactt attagtcagt acgaaaaaga atatttagga ttttatgttt
     5341 cgcaacaccc agtagataaa aagtttgttg ccaaacaata tttaacgata tttaaattga
     5401 gtaacgcgca gaattataaa cctatattag tacagtttga taaagttaaa caaattcgaa
     5461 ctaaaaatgg tcaaaatatg gcattcgtca cattaaatga tggcattgaa actttagatg
     5521 gtgtgatttt ccctaatcag tttaaaaagt acgaagagtt gttatcacat aatgacttgt
     5581 ttatagttag cgggaaattt gaccatagaa agcaacaacg tcaactaatt ataaatgaga
     5641 ttcagacatt agccactttt gaagaacaaa aattagcatt tgccaaacaa attataatta
     5701 gaaataaatc acaaatagat atgtttgaag agatgattaa agctacgaaa gagaatgcta
     5761 atgatgttgt gttatccttt tatgatgaaa
//
LOCUS       DAMQYT010000160         5762 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMQYT010000160 DAMQYT010000000
VERSION     DAMQYT010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.162"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            37..921
                     /gene="ylqF"
                     /locus_tag="PGA33_002157"
     CDS             37..921
                     /gene="ylqF"
                     /locus_tag="PGA33_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDG6473958.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            905..1672
                     /locus_tag="PGA33_002158"
     CDS             905..1672
                     /locus_tag="PGA33_002158"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDG6473959.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            1781..2947
                     /gene="sucC"
                     /locus_tag="PGA33_002159"
     CDS             1781..2947
                     /gene="sucC"
                     /locus_tag="PGA33_002159"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDG6473960.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            2969..3877
                     /gene="sucD"
                     /locus_tag="PGA33_002160"
     CDS             2969..3877
                     /gene="sucD"
                     /locus_tag="PGA33_002160"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDG6473961.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYESLLTVNK"
     gene            4104..5222
                     /locus_tag="PGA33_002161"
     CDS             4104..5222
                     /locus_tag="PGA33_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDG6473962.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQD
                     IKISNTPKNSSQGNNLVINNNELTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKP
                     KTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGA
                     KDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKF
                     QSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA"
     gene            5250..>5762
                     /gene="fmhC"
                     /locus_tag="PGA33_002162"
     CDS             5250..>5762
                     /gene="fmhC"
                     /locus_tag="PGA33_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HDG6473963.1"
                     /translation="MKFSTLSEEEFTNYTKKHFKHYTQSIELYNYRNKINHEAHIVGV
                     KNDKNEVIAACLLTEARIFKFYKYFYSHRGPLLDYFDAKLVCYFFKELSKFIYKNRGV
                     FILVDPYLIENLRDANGRIIKNYNNSVIVKMLGKIGYLHQGYTTGYSNKSQIRWISVL
                     DLKDKDENQLL"
BASE COUNT         2194 a          748 c         1154 g         1666 t
ORIGIN      
        1 aatgagaaaa gaattgacga aagtaaggtg aattgaatgg ttattcaatg gtatccagga
       61 catatggcga aagccaaaag agaagtaagt gaacaattaa aaaaagtaga tgtagtgttt
      121 gaactagtag atgcaagaat tccatatagt tcaagaaacc ctatgataga tgaagttatt
      181 aaccaaaaac cacgtgttgt tatattaaat aaaaaagata tgtctaattt aaatgagatg
      241 tcaaaatggg aacaattttt tattgataaa ggatactatc ctgtatcagt ggatgctaag
      301 cacggtaaaa atttaaagaa agtggaagct gcagcaatta aggcgactgc tgaaaaattt
      361 gaacgcgaaa aagcgaaagg acttaaacct agagcgataa gagcaatgat cgttggaatt
      421 ccaaatgttg gtaaatccac attaataaat aaactggcaa agcgtagtat tgcgcagact
      481 ggtaataaac caggtgtgac caaacaacaa caatggatta aagttggtaa tgcattacaa
      541 ctattagaca caccagggat actttggcct aaatttgaag atgaagaagt cggtaagaag
      601 ttgagtttaa ctggtgcgat aaaagatagt attgtgcact tagatgaagt tgccatctat
      661 ggattaaact ttttaattca aaatgattta gcgcgattaa agtcacatta taatattgaa
      721 gttcctgaag atgcagaaat catagcgtgg tttgatgcga tagggaaaaa acgtggctta
      781 attcgacgtg gtaatgaaat tgattacgaa gcagtcattg aactgattat ttatgatatt
      841 cgaaatgcta aaataggaaa ttattgtttt gatattttta aagatatgac tgaggaatta
      901 gcaaatgacg ctaacaatta aagaagttac gcagttgatt aatgcggtta atacaataga
      961 agaattagaa aatcatgaat gctttttaga tgagcgaaaa ggtgttcaaa atgccatagc
     1021 taggcgcaga aaagcgttag aaaaagaaca agctttaaaa gaaaagtatg ttgaaatgac
     1081 ttactttgaa aatgaaatat taaaagagca tcctaatgct attatttgtg ggattgatga
     1141 agttggaaga ggacctttag caggtccagt cgttgcatgc gcaacaattt taaattcaaa
     1201 tcacaattat ttgggccttg atgactcgaa aaaagtacct gttacgaaac gtctagaatt
     1261 aaatgaagca ctaaaaaatg aagttactgc ttttgcatat gggatcgcga cagctgaaga
     1321 aatagatgaa tttaatattt ataaagctac tcaaatcgcc atgcagcgag ctattgatgg
     1381 attatcagta caaccaacgc atttattgat agacgcgatg acgcttgata atgcactgcc
     1441 tcaagtatct ttaatcaagg gtgatgcaag aagtgtgtcc attgcagctg caagtatcat
     1501 ggcaaaggtt tttcgtgatg attatatgac acagttatct aaagattatc ctgaatatgg
     1561 ttttgaaaaa aacgcgggtt acggtaccaa acaacattta ctagcaatcg atgatattgg
     1621 cattatgaaa gagcatagaa aaagctttga acctataaaa tcgttactgt aaatcgtaaa
     1681 attcaattaa atgataaaaa agtagcattt aagtttacaa tagcgcttac attttttata
     1741 attgtaaggg aacttaacct aagtaacagg aggatggaag atgaatatcc acgagtatca
     1801 aggtaaagaa atatttcgtt caatgggcgt tgcagttcca gaaggacgag tagcatttac
     1861 tgctgaagaa gcggtggaga aagcaaaaga attaaattct gatgtttatg ttgtaaaagc
     1921 acaaattcat gctggaggta gaggtaaagc aggcggagta aaaattgcta aatctttatc
     1981 tgaggtagaa acatatgcaa aagaattatt agggaaaact ttggtgacac atcaaactgg
     2041 tccagaaggt aaagaaatta agcgtttata tatcgaagaa ggttgtgcta ttcaaaaaga
     2101 atattacgtt ggattcgtta ttgatcgtgc gactgaccaa gtaacattga tggcgtctga
     2161 agaagggggc actgagattg aagaagttgc tgcgaagact cctgaaaaga tcttcaaaga
     2221 aactatcgat ccagtaatcg gactttcacc attccaagca agacgaattg cgtttaatat
     2281 taatattcct aaagaatctg ttaacaaagc tgctaaattc ttattagcac tttataatgt
     2341 attcattgaa aaagattgtt caatcgtaga aatcaaccca ttagttacaa cagctgatgg
     2401 tgatgtattg gcattagatg ctaaaattaa ttttgatgat aatgcattat tcagacataa
     2461 agatgttgta gaattacgtg atttagaaga agaagatccg aaagagattg aagcgtctaa
     2521 acatgattta tcatacattg cattagatgg tgacatcgga tgtatggtta atggtgcagg
     2581 tttagccatg gcaacaatgg atacgattaa tcatttcggt ggaaacccag ccaatttctt
     2641 agatgcaggc ggaagcgcta ctagagaaaa agtaactgaa gcatttaaaa tcattttagg
     2701 tgatgaaaat gttaaaggta tttttgtaaa cattttcggt ggcattatga aatgtgatgt
     2761 tatcgcagaa ggtatcgttg aagctgtaaa agaagtagat ttaactttac cactagttgt
     2821 acgcctagaa ggtacaaatg ttgagttagg taaaaaaatc ttaaaagact caggattagc
     2881 aattgaacca gcagcaacaa tggctgaagg tgcacaaaaa attgttaaac tagtcaaaga
     2941 agcataagga aaggatggga gcactaagat gagtgtattt atagataaga atactaaagt
     3001 aatggtacaa ggtattacag ggtctactgc ccttttccat acaaaacaaa tgcttgatta
     3061 tggtacgaaa atagtagcag gtgtgacgcc tggtaaaggt ggtcaagttg ttgaaggcgt
     3121 tcctgttttc aacactgttg aagaagctaa aaatgaaact ggggcaacgg tttcagtcat
     3181 ttacgttcca gcaccatttg ctgcagactc aattttagaa gcagctgatg cagacttaga
     3241 tatggttatt tgtatcactg aacatattcc tgtattagac atggttaaag ttaaacgcta
     3301 cttacaaggt agaaaaacac gtttagttgg tccgaactgt ccaggtgtga ttacagcaga
     3361 tgaatgtaaa attggtatta tgcctggcta tattcacaaa aaaggtcatg ttggtgtagt
     3421 atctcgttca ggtacattaa catatgaagc agtgcaccaa ttgactgaag aaggtattgg
     3481 tcaaactaca gctgttggta ttggtggaga cccagtcaac ggaacaaact ttattgatgt
     3541 tttaaaagca ttcaatgaag atgacgaaac gaaagcagtt gttatgattg gtgaaatcgg
     3601 tggtacggct gaagaagaag cagctgaatg gattaaagcg aatatgacaa aaccagttgt
     3661 aggctttatc ggtggacaaa cagcacctcc tggaaaacgt atgggacatg ctggtgcaat
     3721 catttcaggt ggtaaaggta ctgctgaaga gaaaattaaa acattaaata gttgtggtgt
     3781 gaaaacagcg gcaacacctt cagaaattgg ttcaacatta attgaagctg ctaaagaagc
     3841 aggtatttat gaatcattat taactgttaa taaataaagt taaaagatga tataaatggc
     3901 tatagcccat tccatacttt ataaaagtat cggaaatggg ctatttgtcg tgcgatgaat
     3961 tttagtacac ttttcatatt ttggaaagac aagttaatta ttttacctcc tacaatagct
     4021 ttataaaaca aagaaaattt taattaaaat atatagcgca ctatgttata atgtttgtat
     4081 attattgtaa ggagtgtttc atcatgaata aacaacaaag taaagtacgc tattcaatta
     4141 gaaaagttag tattggaatt ttgtcaattt caataggtat gtttttggca ttgggtatgt
     4201 cgaacaaagc atatgcagat gaaattgata aatctaaaga ttttacaaga gggtatgagc
     4261 aaaatgtatt cgcgaaatca gagttaaatg ctaataaaaa tacgacaaaa gacaaaataa
     4321 aaaatgaagg tgctgttaaa acatcggaca caagtttaaa gttagacaac aaatcagcaa
     4381 tttcaaacgg aaatgaaatt aatcaagata taaagatttc aaatactccg aaaaactcaa
     4441 gccaaggtaa caatctagtt attaataaca atgaacttac taaagaaatt aaaattgcaa
     4501 acttggaagc tcaaaattct aatcagaaga aaacgaataa agttactaat aattactttg
     4561 gttactacag ttttagagaa gctccaaaaa cacaaatcta tactgtaaaa aaaggagaca
     4621 cacttagtgc tatagcatta aaatacaaaa ctacagtttc aaatattcaa aatacaaata
     4681 atatagcaaa tcctaattta atatttattg gtcaaaaatt aaaagtgcca atgacaccat
     4741 tagtagaacc aaaaccaaaa acagtgtctt caaataataa aagtaatagt aatagcagta
     4801 cattaaatta tttgaaaaca ttagagaata gaggatggga tttcgacggt agttatggat
     4861 ggcaatgttt cgatttagtt aatgtatatt ggaatcatct ttatggtcat ggattaaaag
     4921 gatatggagc taaagatata ccatatgcaa ataattttaa tagtgaagct aaaatttatc
     4981 acaacacacc aactttcaaa gctgaacctg gggacttagt ggtttttagt ggaagatttg
     5041 gtggaggata tggtcataca gctattgtct taaatggtga ttatgatgga aaattaatga
     5101 agttccaaag tttagatcaa aactggaata atggtggatg gcgtaaagca gaggttgcac
     5161 ataaagttgt tcataattat gaaaatgata tgatttttat tagaccattt aaaaaagcat
     5221 aatttaaatc gaaggcagga cattgaaata tgaaattttc aactttaagt gaagaagaat
     5281 ttaccaacta caccaaaaag cacttcaaac attatacaca gtctatagaa ttatataatt
     5341 atagaaataa aataaatcat gaagcacata ttgtgggagt gaagaatgat aaaaatgaag
     5401 ttatagctgc atgtttatta acagaggcac gaatttttaa attctacaaa tatttctact
     5461 ctcatagagg tcctttactt gattatttcg atgctaaatt agtttgttac ttttttaaag
     5521 aattatctaa attcatttat aaaaatagag gagtatttat tcttgttgat ccatatttaa
     5581 tagagaattt aagagatgca aatggtagga taataaagaa ttataataat tcagtgatag
     5641 taaagatgct agggaaaatt gggtatctcc atcaaggtta tacaacagga tattcaaata
     5701 aaagtcaaat taggtggatt tctgtattgg atttaaaaga taaagatgag aatcaacttt
     5761 ta
//
LOCUS       DAMQYT010000161         5707 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMQYT010000161 DAMQYT010000000
VERSION     DAMQYT010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.163"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            45..1046
                     /gene="argF"
                     /locus_tag="PGA33_002163"
     CDS             45..1046
                     /gene="argF"
                     /locus_tag="PGA33_002163"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDG6473964.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEIMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            1069..2001
                     /gene="arcC"
                     /locus_tag="PGA33_002164"
     CDS             1069..2001
                     /gene="arcC"
                     /locus_tag="PGA33_002164"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDG6473965.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            2173..3729
                     /locus_tag="PGA33_002165"
     CDS             2173..3729
                     /locus_tag="PGA33_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDG6473966.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
     gene            4037..4264
                     /locus_tag="PGA33_002166"
     CDS             4037..4264
                     /locus_tag="PGA33_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473967.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
     gene            complement(4569..5516)
                     /locus_tag="PGA33_002167"
     CDS             complement(4569..5516)
                     /locus_tag="PGA33_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDG6473968.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLKNVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLLLAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPDIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKEKQDKFLRNASQ"
BASE COUNT         2002 a          778 c         1076 g         1851 t
ORIGIN      
        1 cgcttacaat ttaggtgtaa ctaaaataaa aggagttgtt attaatgaaa aatttacgaa
       61 acagaagttt tttaacttta ttagactttt cacgacaaga ggtagaattc ttattaacac
      121 tctccgagga tttaaaacgt gctaaatata ttggcactga aaagcctatg ttaaaaaata
      181 aaaatattgc actgttattt gaaaaagatt ctacaagaac gcgatgtgca tttgaagttg
      241 cagcgcatga tcaaggtgca aatgtaactt atttaggccc aactggatca caaatgggta
      301 aaaaagaaac aactaaagat actgcacgtg tgcttggtgg aatgtatgat ggcattgaat
      361 accgtggttt ttcacaaaga acagtagaaa ctttagctga gtattcaggc gtaccagtgt
      421 ggaatggttt aactgatgaa gatcatccta ctcaagttct tgctgatttc ttaacagcaa
      481 aagaagtctt aaaaaaagat tatgcagata ttaactttac atatgttgga gatggtcgta
      541 ataacgttgc aaatgcatta atgcaaggtg ctgccattat gggtatgaac ttccatttag
      601 tttgtccaaa agaattaaat ccaacagatg aattattaaa tcgctgtaaa aatattgccg
      661 ctgaaaatgg tggcaacata ttaatcacag atgatattga ccaaggtgta aaaggttcgg
      721 atgtaattta cactgatgtt tgggtatcaa tgggtgaacc tgatgaagta tggaaagaac
      781 gacttgaatt attgaaacca tatcaagtaa ataaagaaat aatggataaa actggtaatc
      841 caaatgttat ttttgagcat tgcttaccat ctttccataa tgctgatacg aaaataggtc
      901 aacaaatttt tgaaaaatat ggtattcgag aaatggaagt tacagatgaa gtattcgaaa
      961 gtaaagcttc agttgtattc caagaagctg agaacagaat gcatacaatc aaagcagtca
     1021 tggttgctac attgggtgaa ttttaaatga tataaggaag tgaatatgat ggcgaaaata
     1081 gtagtagcat taggtggtaa tgctttagga aaatcacctc aagaacaact cgagcttgtt
     1141 aaaaatactg cgaaatcatt agtaggatta ataacaaaag gacatgagat tgttattagt
     1201 catggtaatg gaccacaggt tggaagcatt aatttgggac ttaactatgc tgcagaacat
     1261 aaccaaggtc cggcatttcc atttgctgaa tgtggcgcaa tgagtcaagc ttacatcggc
     1321 tatcaattac aagaaagctt acaaaatgaa ttgcattcta ttggaatgga taaacaagtg
     1381 gtaacactag tgacacaagt tgaagttgat gaaaatgatc cggcatttaa caatccttca
     1441 aaaccaattg ggttatttta caacaaagaa gaagctgaac aaattcaaaa agaaaaagga
     1501 tttatatttg ttgaagatgc tggaagagga tatagacgcg ttgttccttc accacaaccc
     1561 atctctatta ttgaattaga gagtattaaa acacttatta aaaatgatac actcgttatt
     1621 gctgctggtg gtggaggtat accagtaatt agagagcaac atgatggttt taaaggtatt
     1681 gatgcagtta tagacaaaga taaaacaagt gcgttgttgg gtgctaatat tcaatgcgat
     1741 caattgatta ttttaacagc aattgattat gtatatatta attttaacac tgaaaaccaa
     1801 cagcctttga aaacaacaaa tgttgatgaa ttaaaacgat atatagacga aaatcaattt
     1861 gcaaaaggaa gtatgttacc aaaaattgaa gcagccatat catttattga aaacaatcca
     1921 aaaggaagtg tgcttataac atcattaaat gaattagatg ctgccttaga gggtaaagta
     1981 ggtactgtga ttaaaaagta attgaattga aacgcttttc aattactata tgtcaaatgc
     2041 atgattttta ttatttatgt gcaccccctg aaaataatgc ctctattttg atgcggggtg
     2101 cactttctta atttatattt ataaaatctt taaggtagaa atgctaggtt aaatgtcgaa
     2161 ggagatgaaa ccgtggaaaa tacaattaat gaaagtgaaa agaaaaaacg atttaaatta
     2221 aaaatgccag gtgcatttat gattttattc attttaacgg ttgttgcagt tatagcaaca
     2281 tgggttattc ctgctggtgc atattctaaa ctttcttacg aaccttcatc ccaagaacta
     2341 aagatagtta accctcataa ccaagtgaaa aaggttccgg gtacgcaaca ggaactagac
     2401 aaaatggggg ttaaaattaa gattgaacaa tttaaatcag gtgcaattaa taagccggta
     2461 tcaattccga atacttatga aagattaaag caacatccag ctggaccaga acaaataaca
     2521 agtagcatgg ttgaaggtac gatagaagcg gtcgatatca tggtattcat tcttgtacta
     2581 gggggactta ttggcgtagt tcaagccagt ggttcttttg aatcgggatt gttagcttta
     2641 acgaagaaaa caaaagggca tgaatttatg ctaattgtgt ttgtatcaat actaatgatt
     2701 atcggcggga cattatgtgg tattgaagaa gaagctgtag cattttatcc gattttagtc
     2761 cctatattta tagcgttagg atacgattct atcgtttcag ttggtgccat attccttgcc
     2821 agctctgtcg gtagtacatt ttcaactatt aacccgttct cggttgtaat tgcctctaat
     2881 gccgctggta caacttttac ggatggcttg tattggagaa taggtgcttg tattgtcggt
     2941 gcgatttttg ttattagtta tttatattgg tactgtaaaa aaattaaaaa cgatcctaaa
     3001 gcgtcatatt cttatgaaga caaagatgct tttgaacagc aatggtctgt attaaaagat
     3061 gacgatagtg cccattttac tttgcgtaag aagataatcc ttacattatt tgtactacca
     3121 tttccaatta tggtatgggg agttatgacg caaggttggt ggttcccagt tatggcttca
     3181 gcatttttaa tatttacaat tataataatg tttattgctg ggacaggtaa atctggattg
     3241 ggagaaaaag gaactgtaga tgcatttgtc aatggtgcat caagtttagt aggtgtatct
     3301 ttaattattg gtttagctcg aggtattaat ttagtgttga atgaaggtat gatttcagat
     3361 acaatcttac acttttcatc atctttagtt caacatatga gtggaccatt atttatcatc
     3421 gtattactat ttattttctt ctgtttaggt tttatcgtgc catcatcttc tggcttagcg
     3481 gtattatcaa tgcctatctt tgcaccacta gctgatacag taggtatacc aagattcgtc
     3541 atcgttacga catatcaatt tggtcaatat gcgatgttat tcttagcgcc gactggactt
     3601 gttatggcca cactacaaat gttaaacatg cgatattcac attggttccg atttgtatgg
     3661 ccggtagttg cttttgtatt gattttcggt ggcggagtac taattacgca agtactaatt
     3721 tattcataat ttgaaatgct atattataaa aaatactaat ggggttttat gcatctcgta
     3781 ggtttgtaga aatactaaac taagcgaggt gcattattat ttttgattaa gaaaataatg
     3841 acggtaatga taacactagt aagtaattga tacaatgctc tatttaataa tgatattttt
     3901 aaaatttgtt tttaatgtaa tgttagatct atggtatatt atatttatcg tggtaaatat
     3961 gtatttgctg taaacagttt ttgtaacaca tgatacatat ggttgtgaaa atagcatata
     4021 taaaggatgg ctataaatga cacatttgac aaaggtttta gatacactaa ctggaatatg
     4081 cgtagtatta ttatttagta aatattttgt ggcgtatgca aatatggtgt ttgattggaa
     4141 tttaagatgg tatttgctag aaaacatacc acatttgcca attatattat ttattctgat
     4201 gtttattttc ggagtacctt ctgaaatgat aaaagatagg caaaggaaaa ataacggtgt
     4261 ttaatttatc gatatttaga ggtgataaaa tttgtcaact attacaaaga ctaaaataaa
     4321 aatctttatt aattatttta ttggtatatt agccttggcg tttgctgctt atatattatt
     4381 taaataaagt ttagagtgaa acgtgtttat gaactagacg ttctagtata gttactatag
     4441 ctttagtcag aaggtatcat tgataagatc atattaaatc aaagaggcat tgatatacac
     4501 taaaaagagg caagattacc tgcctctttt tagttattaa atatacgtgt taattcttgg
     4561 taatagtgtt actgagacgc attacgaaga aatttatctt gtttttcctt ttttaaaaag
     4621 aagtgaagat atcctataaa gactctaagt actattacag tggctaataa cacttcgata
     4681 aacaaaagac ctttccaaat gtctggaaac ataagtacag gcaaactgtt ctttaaagca
     4741 gttgctgaga ttactaaagg gaatgtgaaa gctgaaaata cgggcgaaaa aggttctttt
     4801 agtaatttag gcaattgtat aatgatataa aaataaaata tttgagctaa tagtaaaaat
     4861 ataattacga taaaagcatt cgccttagga aaagctataa catatgctgc agcgactaaa
     4921 gaaaatggtg cacaaattgt cgatgtgttc ggtttgattg acgtctgcaa tggaaatgct
     4981 tttaatcgct tgaaaactat aggtaagaca atacaagtag ctacaaagcc atatattact
     5041 gttaattgac ctatgaaaaa atatccgcta acgggtgccg tcaatcctgc gatagcaata
     5101 ccaataaaaa gtacagtcca cgaaggataa acatttttaa gtgaaaaatc ttttaaatat
     5161 tttattgaaa aaataatcat atgcgtcata attcccacaa ggcataaaat ccaaataggc
     5221 gttattaagc tattgataaa agttatgtta ctaaaaaatg tatttaaata agtagtacct
     5281 aaaaagccag acatgaaaaa tgttgtgaac actgatgaaa ctagaggact gtttaattgt
     5341 tctttcacat tattaaaata tttgatcata gtacataaaa ggtgaatcca aatcaagaaa
     5401 gcaaagattc cgcaaacagc gtttaaagta agagataagt cttttaatag attacccagg
     5461 cctaataatc ctaagactag tcctgacgtt actagaggtg ctttttgaag tctcatgatt
     5521 tacaaccttt cttatgtgat tttcttcact aattatatca tgatagctat ggccaattaa
     5581 taagaagagt gtgtactatt acgttattag agtatgtata ttggattgat agacacataa
     5641 ttgacattta aatctcaaaa ttaatgatat ttgaggtatg ctttgcaacc taaatattgg
     5701 aatatgt
//
LOCUS       DAMQYT010000162         5690 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMQYT010000162 DAMQYT010000000
VERSION     DAMQYT010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.164"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            286..1455
                     /locus_tag="PGA33_002168"
     CDS             286..1455
                     /locus_tag="PGA33_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HDG6473969.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG"
     gene            complement(1562..1990)
                     /locus_tag="PGA33_002169"
     CDS             complement(1562..1990)
                     /locus_tag="PGA33_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDG6473970.1"
                     /translation="MILNFNQFENQNFFNANPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(2206..2652)
                     /locus_tag="PGA33_002170"
     CDS             complement(2206..2652)
                     /locus_tag="PGA33_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDG6473971.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDKK"
     gene            3050..3415
                     /locus_tag="PGA33_002171"
     CDS             3050..3415
                     /locus_tag="PGA33_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDG6473972.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            3597..4874
                     /locus_tag="PGA33_002172"
     CDS             3597..4874
                     /locus_tag="PGA33_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDG6473973.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
     gene            complement(5278..5634)
                     /locus_tag="PGA33_002173"
     CDS             complement(5278..5634)
                     /locus_tag="PGA33_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HDG6473974.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     TTIEKMISEKDESIEKNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
BASE COUNT         1894 a          803 c          965 g         2028 t
ORIGIN      
        1 aaaagtatgt gagtgttcct aaataatatg attaaaatga tggcgaataa gtgtctaaaa
       61 gcatcttaaa gggacattgt atagggtaaa tcacttcata aataagggaa aatccttatg
      121 ttcacttttt cacaatcata ataaaatata tatgtagtca atactttgtc tatattgaat
      181 gttttcatat aaatgaaagc atttttaaat aacattgacc tctaatatat aggcagagta
      241 ttgatatcta ttaaaaaata aatgattttg atgaaggtga aacgtatgta caaaacaaaa
      301 ggtggctttc aacttacatt acaaacatta agtttagtgg ttgggtttat ggcttggagt
      361 ataattgcgc cattaatgcc ctttattaaa caagatgtca atgttactga aggtcaaata
      421 tcaatcattt tagcgatacc agttattttg ggatcggtgc tccgtgtgcc atttggttat
      481 ttaacaaaca ttgttggcgc taaatgggta ttctttacta gttttatcgt attgttattc
      541 ccgatatttt tcttaagcca agcacaaaca ccgggtatgt taatggcttc aggatttttc
      601 cttggtgtag gtggtgcaat tttctcagtt ggtgttacat cagttcctaa atatttccct
      661 aaagaaaaag taggtctagc aaatggtatt tatggtatgg gaaatatcgg tacagcagtt
      721 tcttcatttt tagcaccacc gatagcgggt attattggtt ggcaaacaac agttagaagt
      781 tacttaatta ttatcgcttt atttgcatta attatgttca tttttggtga cacacaagaa
      841 cgtaaaatta aagtaccatt aatggctcaa atgaaaacat tatctaaaaa ctacaaatta
      901 tattacttaa gttattggta ttttattact tttggtgctt ttgtagcatt tggtattttc
      961 ttacctaact acttagtaaa tcattttgga attgacaaag tagatgctgg tattcgatca
     1021 ggtgtattca ttgcgctggc aacattctta agaccaatag gtggcatttt aggtgataaa
     1081 tttaatgcag ttaaagtatt gatgattgat tttgttgtta tgattatcgg tgccattatt
     1141 ttaggtattt cagaccatat cgcattattc actgtaggct gtttaacaat aagtatttgt
     1201 gcaggtattg gtaacggctt aatcttcaaa ttagtaccat catacttctt aaatgaagcg
     1261 ggatccgcaa atggtatcgt atcaatgatg ggtggtttag gaggattctt cccaccacta
     1321 gtaatcacgt acgtagctaa tttaacagga tcaagtcatt tagcatttat tttcttagcg
     1381 gtatttggat gtattgcatt atttaccatg cgtcatttat accaaaaaga atatggctca
     1441 ttgaaaaacg gttgatatgt aatacatgcc attcatttag ttaaatacaa agccttaata
     1501 tcatgcgcaa tattcgtagc atgacattaa ggctttagta gtgtattcat tttaaattta
     1561 actatgaaat tggaatagat gttgatgaag tagtttcatt gcttggtttg attttaggca
     1621 aggtaatggt taataaccca ttttcaaaac tagcagtaat atgttgctta tcaacagctt
     1681 caaaatcaaa ttgacgcatt aatgattcga agttacgctc atctaaaatg agttgttcag
     1741 atttgtattt tgcgcttcta gtagcttgaa tagtgagcgt attattattg aaatcgatac
     1801 taatatcttc tttatttaca cctgctagtt cagcttctaa gtaatataat tcgtctgttt
     1861 catatatatt cgttacaaat gaaggtgttg aaaagtaatt aaatacttgt ttacctaaat
     1921 ctttaaatgt atcacttgga ttagcgttaa aaaagttttg attctcgaat tgattgaagt
     1981 tcaatatcat cacttccatt atagaaattt gtattacaaa gccattatat gagtagtatt
     2041 aaaaaagtta aagtaaaagg ggagataaaa aagtaataaa tgggtagatt tgtgcctgat
     2101 aaggaatcag ggcaaaagaa ctgagcatcg agatttatac aagcaataaa gataatgaat
     2161 aaacatatcg atgcatactt ctgcccatca ccttatgata gggagttatt ttttgtcgga
     2221 atagtcaaaa tttttagaaa caaaattccg caaattatta attatatctg atgcttctcg
     2281 ttctgaaatg ttgaattcat taaagacctt aacagaaatt tcagcaagag ggctttttat
     2341 tgctttacct tgttctgtta gagaaatttg taggtttctt tcatctttct cttcacgtgt
     2401 tcgaacaacg taatctttct tttctaattt ctttagtaat ggtgtcagtg ttccagaatc
     2461 taagaacaca cgttcaccta attttttgat gttaagtttt tcatcatttt caatcgccat
     2521 taaaacaatg taaccagtat aggttaaatc atattcctta agatagtttg tgtatttttt
     2581 aattatttct tttgaagaaa cataaaataa aaaacacaac tgtttgctaa gatagctgtt
     2641 ttctacatac atccaatcac tccttgttaa aataaacaat attatgaagt gtaatagttg
     2701 tcaagtactg atttcatcgc aattattgaa ttgtctataa ttgaatttaa atgttaaata
     2761 atgcaaaata tatcgatatc ttagtatttt catcggtaat tactgatatg actgattttt
     2821 taaattgaaa aaaatgtaat tgtatcatcc ttaattgtta aaagtagtca attattttga
     2881 tagatagtaa aataaaattt gttattaaat tttttaaaag catcgtgtat gtaattcgat
     2941 ttctataata atattgagaa taaaaaaatt tgttacaatc tggtaaaaac tctttattaa
     3001 ataaattgat tcgttcatac tgaatagata agcaaaaagg agttacataa tgaagaagaa
     3061 aaaaaacgcg attaaagtta tcattatatt atttatttca ttaattgttg cagtagcatt
     3121 tttctttggt ctgaaaacat atcaaggcca taagaatatt caactaattg acagttactt
     3181 ggaagagaaa aatctaaaag ataaaattaa atcagaaaaa acagagtata gtgctaaaaa
     3241 aggtctcttc tataaggaag ttacatttaa agatgaacct ggtgtaactt atgttgtgca
     3301 accaatcagt actaataaag gattatttgt tgaaggcttt gatacagaga ctaagaaaag
     3361 tttgaaaact gcaaagcata aatatttcaa tcaaaattat aaaccatcaa aatagtgaaa
     3421 cgttaaactg gtgaagcagt ttagtgcctt caattttaat tatttgaggt aaacagtttt
     3481 tgtatggaaa ttagtgattt ttgacatcta taaaagataa atattttaat tttttataga
     3541 taattatcaa ttttctgata taattgagtg atgtcagatg aaggggagtt tgtactatgg
     3601 ctctattcaa gagaaaaatt agcctaccta tgcaagttgt tattgcatta gtgttaggtg
     3661 ttgtcgtagg acttttatta tatggacaag aaaacgtagc aaattacatt aaaccatttg
     3721 gtgacgtatt tttaaattta attaaaatga tcgttatacc agttgtattt tgctcactag
     3781 cgctttctat ttcgaacgtt ggggaatcga aaactgtagg gcgttatggc tggaaaacaa
     3841 ttttatactt tgaaattatt acaacaatcg caataggttt agggattatc ttcggtaacc
     3901 tatttaaacc aggtgctgga ttagacccaa caaaattacc taaaggtgat atttctaaat
     3961 atcaatcaac tgcacatgca gcagaacaat ctacatatgg aaatcatttt attgatacca
     4021 ttgtacatat tattccgaca aacttttttg aagctttaaa taagggtgaa ttattaccta
     4081 ttatcttctt cgcagtattc tttggattag gattagctgc tgtaggtaaa aaagcagaac
     4141 cagttaaaga atttttaagc ggatcgcttg aagctgtgtt ctggatgatt aataaaattt
     4201 taaaattagc accacttgga gtgtttgcat tcatttgtac tacaattatt acatttggtg
     4261 catccgcatt attaccacta ttaaaattag tattagttgt tgtctttgca atggtgttct
     4321 ttgtattcgc tatactagga ctagttgcat ggatgtgtgg tattaatatc atgaatatta
     4381 ttagaatctt gaaaagtgaa ttgcttttag cattttctac atcaagttcg gaagctgtac
     4441 ttcctgtaat gatgaagaaa atggaaaact tcggttctcc aaaagaaatt acttcttttg
     4501 ttataccaat tggttatacg tttaacttag atggatcagc actttatcaa tctattgcag
     4561 cattattcgt tgcacagatg tatggaatgc acttaacatt atcagagcaa attgtgttga
     4621 tgttaacatt aatgattacg tctaaaggta tggcggcagt accaggaact tcaatcgttg
     4681 ttttattaac aacattaggt gccatgggct taccggcaca aggtttagca ttaattattg
     4741 gtgttgaccg tatcttagat atggtacgta catgtgtaaa cgttattggt aatgcattat
     4801 caacaatcgt tatagctaaa tgggaaaacg tatatgacaa agcaaaaggt caagaatatt
     4861 taaaatcaat ttaaaaaata ctatctgaca tttaaagccc cttacaacct ttggttgtta
     4921 ggggctttta tgcatgcgct aaagcgcagg cgaacggaag cgtagtgacc tcaggttgtt
     4981 agggactttt atgcatgcgc taaagcgcag gcgaaccgaa gcgtagtgac ctcaggttgt
     5041 taggggcttt tatgcatgcg ctaaagcgca ggcgaaccgg agcgcagtga cctcaggttg
     5101 ttaggggctt ttatgcatgc gcaaagcgca ggcgaacggc aagtggagct attcaatatg
     5161 aaagtatttc aaatataaaa agcagcagta aaatgattcc aaattggaaa tcaacttacc
     5221 gctgctttta ctcgtatata ccttgatggc aactaaaatt tgtaatttaa attacaatta
     5281 gtgtttcatg tgcattttgc ccatgtcatg aattttcatc attaaaccat gtggtacatc
     5341 gtcatggtca acttttttaa ctttaacaat tttatctgaa tctgctaatt tataaacaat
     5401 tacaaaatca ccttttttaa ctttaacatc tgaatctgaa ggtaatttaa catttttttc
     5461 aatactttca tctttttcac taatcatttt ttcaatagta gtattatctt tcataattcc
     5521 atagtatgta tctttctttc cgccagtcat catcatgatt actaatacaa tacatacaat
     5581 taacatgatg gcgattaaac taataccaag accaaaacct tttttctttt tcatggttca
     5641 tcaatctcct taaatgtagt taattctgtt atacctaaaa gtgcaacagt
//
LOCUS       DAMQYT010000163         5546 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMQYT010000163 DAMQYT010000000
VERSION     DAMQYT010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.165"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(142..1194)
                     /gene="isdA"
                     /locus_tag="PGA33_002174"
     CDS             complement(142..1194)
                     /gene="isdA"
                     /locus_tag="PGA33_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HDG6473975.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
     gene            1403..2086
                     /gene="isdC"
                     /locus_tag="PGA33_002175"
     CDS             1403..2086
                     /gene="isdC"
                     /locus_tag="PGA33_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HDG6473976.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            2086..3162
                     /gene="isdD"
                     /locus_tag="PGA33_002176"
     CDS             2086..3162
                     /gene="isdD"
                     /locus_tag="PGA33_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HDG6473977.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTIKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            3159..4037
                     /gene="isdE"
                     /locus_tag="PGA33_002177"
     CDS             3159..4037
                     /gene="isdE"
                     /locus_tag="PGA33_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HDG6473978.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            4050..5015
                     /gene="isdF"
                     /locus_tag="PGA33_002178"
     CDS             4050..5015
                     /gene="isdF"
                     /locus_tag="PGA33_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HDG6473979.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            5077..>5546
                     /gene="srtB"
                     /locus_tag="PGA33_002179"
     CDS             5077..>5546
                     /gene="srtB"
                     /locus_tag="PGA33_002179"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HDG6473980.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQEHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKII"
BASE COUNT         2019 a          721 c          977 g         1829 t
ORIGIN      
        1 gtattgtaca ttgaaatgtt agttaattgc aacgttattt tataaaaaaa taacctatgt
       61 tatagattat gaaatatcta tgacataggt taaataaaat ttaaaataaa aaatatatta
      121 agttatgagt gtgacgatga tttatttaga ttcttttctt ttgaaaagta ataaagataa
      181 tgaacctaaa agggcaagtg ttgcgaaggc aactgtgcta ataaagttat caactgaagt
      241 taaaccagtt tttggtaatt ctttagcttt agatgcttgt ttaggcgttt cgttatgttt
      301 tgtaactttg ttagtttgtt gtgatttatt atcacttaca gcttgattgt tgctttcaga
      361 tttcgctgtt gcaacatctt taacaggtgt ttgaacttta ttttgttctt gagcagtttg
      421 tgctgtttta actgtatgag cagtttgtgt tttagtttga tcttttgttg tgtcagtact
      481 tacaacttta gtagagtgat tgtcttcaac tttgcttgtt gtagttacag taggtttaac
      541 tttttcaact ttaggttgaa ctggtttagt ttgctcagta ggtgttttag gttgagctgg
      601 ttttggttga accggtttaa cattgtttgg ttttgctgcg tcagctaatg taggaattgc
      661 tttttcaaat tccaaatgcg tagtatatct atgattgtaa ttaatttgtg gcacgacaat
      721 atgtacttta gtagttaagc tcttatatcc aggttcaact gcaacattga ttgttctagt
      781 atccgctttt ttattatcgt taacaacagt tgttgctaat tcttgattgt ttgcattgta
      841 aaatttgtat tctttccaga atgatgcatt gtttaacacg gtttggaaat aatatttatt
      901 attttgttta attactttac cagggtgttg catatagtca tccatgtgtg acttctctga
      961 agagccatct ttttgcactt ggaaattaat tggttgtgat gttgcttgag aaacttgtgt
     1021 gctttgatta ttagttgcgt tcgtagcttc tgttgccgca ttgacttgtt ggctgtctgc
     1081 gcctatgtat acaagggaac ctaaaatgat agatgctgta cccattgtaa tctttttcat
     1141 agctgatgaa cgttgttctg attgatactt actgtttaaa taatgttttg tcatgttgtt
     1201 ttcctcctaa ggatacaaat tatgtgttgt tatatgagat gattaaccat tgcaattgat
     1261 aatggttatc aattatacta gcacatctat tttcgtataa caataataaa ttgattataa
     1321 aataaatatt gacaatgata atcattatta tttatgattt tactaaagac ttaaaagcaa
     1381 tcataaaaag gaggattatg ttttgaaaaa tattttaaaa gtttttaata caacgatttt
     1441 agcgttaatt atcatcatcg cgacattcag taattctgca aatgccgcag atagcggtac
     1501 tttgaattat gaggtttaca aatacaatac caatgacacg tcaattgcta atgactattt
     1561 taataaaccg gcaaagtaca ttaagaaaaa tggtaaattg tatgttcaaa taactgtcaa
     1621 ccacagtcat tggattactg gaatgagtat cgaaggacat aaagaaaata ttattagtaa
     1681 aaacactgcc aaagatgaac gcacttctga atttgaagta agtaagttga acggtaaaat
     1741 agatggaaaa attgacgttt atatcgatga aaaagtaaat ggaaagccat tcaaatatga
     1801 ccatcattac aacattacat ataaatttaa tggaccaact gatgtagcag gtgctaatgc
     1861 accaggtaaa gatgataaaa attctgcttc aggtagtgac aaaggatctg atggaacgac
     1921 tactggtcaa agtgaatcta atagttcgaa taaagacaaa gtagaaaatc cacaaacaaa
     1981 tgctggtaca cctgcatata tatatgcaat accagttgca tccttagcat tattaatcgc
     2041 aatcacattg tttgttagaa aaaaatctaa aggcaatgtg gaataatgag aaatgttaaa
     2101 caaattgcta caaaatctat tatagctatt attagcttag gtatacttac atatacaaca
     2161 atgattggta gcgtgttggc tgatgagata aaatatccat cagccaaatt taatcaacct
     2221 gaagcaaaag ataaaacaga attaactaca tcaatttttg atgaaaagat aaaagagaat
     2281 aaagcgttag agttactaat ttttaatcaa gaaaataaaa atgtaactga ggaacaacaa
     2341 ctagttgacg aaaaggcgca attgatttca gatatgactg gtaaaattta cttgcaagta
     2401 aagctaaaag gtcaaataga taaagaacaa cttgtttttc aaaatgacaa aaatgaagaa
     2461 tttccttttg ttataaaaga tgaaaaggat gacacaatag taagaatttt aattgaacag
     2521 catatggata aaatcaatat gcatgttaaa acgttggctg aaaagaaaaa tctagataac
     2581 aaagaaatgg tgtattctat tcattttaaa gagaaaaaag tacaacatga tgatgcaaaa
     2641 gaagtgcctt caaaacatca aaatcaagaa aataatcaag atcagcttaa aaaagatatt
     2701 gatgacaaaa aagatagtca aaaatcagat actaaggaaa gacgtactag cctttttact
     2761 gaaaaaggat taaatgatat tcctgtacaa aaagataaag tgcaacaaga cagtaataaa
     2821 aagattgaaa atgagcgacc taaagcatca ggtacattaa aagttgaaaa tagccctcca
     2881 acaataaaaa aggttgaaaa taatcacaaa gagcaaccga aacataaaga tgaaaaatca
     2941 aaaaaggaaa agaaaaaagt agttgaaaaa gaaaaagcgt taccagcttt taatagagat
     3001 gatgatagca agaatagtag tcaattatct agtgatatta aagaacttga tgaaccaaat
     3061 cataaaaagc aatatatgtt atttgcagct ggcattgtgt tagcaactat tttacttatt
     3121 tcggcacatt tatacagcag aaagagaggt aaccaagttt gagaatcata aagtatttaa
     3181 ccattttagt gataagcgtc gttatcttaa ccagctgtca atcttccagt tctcaagaat
     3241 caactaaatc cggcgaattc agaatcgtac caacaactgt tgcattgaca atgacattgg
     3301 acaaattgga tttaccaatt gtcggcaaac ccacgtcata taagacattg cctaatcgtt
     3361 ataaagatgt accggaaatt ggtcaaccaa tggagccgaa tgttgaagct gttaaaaagt
     3421 taaaaccaac acatgttttg agtgtgtcaa cgattaaaga tgaaatgcaa ccattttaca
     3481 aacaattaaa tatgaaaggc tacttttatg attttgatag tttaaaaggg atgcaaaagt
     3541 cgattacaca attaggtgat caatttaatc gtaaagcaca agcaaaagaa ttaaatgacc
     3601 atttaaattc tgtaaagcaa aaaattgaaa ataaagcagc taaacaaaag aaacatccca
     3661 aagtattaat attaatgggt gtaccgggta gctatttagt agcaactgat aaatcatata
     3721 ttggtgattt agttaaaata gcaggtggag aaaatgttat taaagtgaaa gatcgtcaat
     3781 atatttcgtc taatactgaa aatttgttga atatcaatcc agatattatt ttacgattac
     3841 cacacggaat gcctgaagaa gttaagaaaa tgtttcaaaa agaatttaaa caaaatgata
     3901 tttggaaaca ttttaaagct gtgaaaaata atcatgttta tgacttagag gaagtgccat
     3961 tcggtattac agcaaatgtt gatgctgata aggcaatgac tcaattatat gatttatttt
     4021 ataaggataa aaaatagtga gttgatatga tgataaaaaa taaaaagaaa ctactatttt
     4081 tatgtttgtt agtcatttta atcgcaactg cttatatttc gtttgtaacc ggtacaatta
     4141 aattgtcatt taatgaccta tttacaaaat ttacaactgg tagcaatgaa gcagtggatt
     4201 caatcattga tttgcgattg ccacgtatat taattgcatt gatggttggc gcaatgttag
     4261 cagtttctgg agcattatta caagcagcac tacaaaatcc tttggcagag gcgaatatca
     4321 ttggcgtttc ctcaggtgca cttataatga gagcactttg tatgttgttt attccacaat
     4381 tgtactttta cttaccatta ttaagtttta ttggaggttt aataccattt ttaataatta
     4441 tattgttgca ttctaaattt agattcaatg ctgtaagtat gatattagta ggtgttgcgt
     4501 tattcgtatt attaaatggt gttttagaaa ttttaactca aaacccttta atgaaaattc
     4561 ctcaaggctt aacaatgaaa atatggagcg acgtatacat attagcagta tcagcattat
     4621 tgggattaat attaacatta ctattgtccc ctaaattgaa tttactaaat ttagacgaca
     4681 tacaagcgcg aagtatcggt tttaatattg atcgttacag atggttaaca ggtttattag
     4741 cagtattttt agcaagtgca actgttgcga ttgttggaca actagccttt ttaggtatta
     4801 ttgtgccaca tgtggttaga aagctagttg ggggcaatta cagagtactt attccgtttt
     4861 ctacagttat tggtgcatgg ctattgttag tggctgattt attaggacga gtgatacagc
     4921 ctcctttaga aattccagcc aatgctattt taatgattgt cggtggtcca atgctaattt
     4981 acttaatttg tcaaagtcaa cgaaatcgaa tctaaaacgg tacaacaact ggtttagtga
     5041 tgactgaaga aacgtcgtta ataaaagagg atttttatga gaatgaagcg atttttaact
     5101 attgtacaaa ttttattggt tgtaattatt atcatttttg gttacaaaat tgttcaaaca
     5161 tatattgaag acaagcaaga acgcgcaaat tatgagaaat tacaacaaaa atttcaaatg
     5221 ctgatgagca aacatcaaga acatgtgaga ccacaatttg aatcacttga aaaaataaat
     5281 aaagacattg ttggatggat aaaattatca ggaacatcat taaattatcc agtactacaa
     5341 ggtaagacaa atcacgatta tttaaattta gattttgagc gagaacatcg acgtaaaggt
     5401 agtattttta tggattttag aaatgaattg aagaatttaa atcataatac tattttatac
     5461 gggcaccatg tcggtgataa tacgatgttt gatgtgttag aagattattt aaagcaatcg
     5521 ttttatgaaa aacacaagat aattga
//
LOCUS       DAMQYT010000164         5486 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMQYT010000164 DAMQYT010000000
VERSION     DAMQYT010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.166"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            129..296
                     /locus_tag="PGA33_002180"
     CDS             129..296
                     /locus_tag="PGA33_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473981.1"
                     /translation="MRKIWVIDCLYASTFFVDCLTVDSHLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
     gene            293..2716
                     /gene="hysA"
                     /locus_tag="PGA33_002181"
     CDS             293..2716
                     /gene="hysA"
                     /locus_tag="PGA33_002181"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase HysA"
                     /protein_id="HDG6473982.1"
                     /translation="MTYRIKKWQKLSTITLLMAGVITLNGGEFRSVDKHQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYDKYDENNPDMKKKFDATEKEATNLLKEMKTESGRKYLWS
                     GAETLETNSSHMTRTYRNIEKIAEAMRNPKTTLNTDENKKKVKDALEWLHKNAYGKEP
                     DKKVKELSENFTKTTGKNTNLNWWDYEIGTPKSLTNTLILLNDQFSNEEKKKFTAPIK
                     TFAPDSDKILSSVGKAELAKGGNLVDISKVKLLECIIEEDKDMMKKSIDSFNKVFTYV
                     QDSATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNDT
                     TLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDAMDDSTK
                     AKYKKIVKSSVESDSSYKQNDYLNSYSDIDKMKSLMTDNSISKNGLTQQLKIYNDMDR
                     VTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYHDNFWV
                     TADMKRLSGTTTLDNEILKDTDDKKSSKTFVGGTKVDDQHASIGMDFENQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTNSDNQENNSVF
                     LESTDTKKNIGYHFLNKPKITVKKESHTGKWKEINKSQKDTQKTDEYYEVTQKHSNSD
                     NKYGYVLYPGLSKDVFKTKKDEVTVVKQEDDFHVVKDNESVWAGVNYSNSTQTFDINN
                     TKVEVKAKGMFILKKKDDNTYECSFYNPESTNSASDIESKISMTGYSITNKNTSTSNE
                     SGVHFELTK"
     gene            complement(2776..3630)
                     /locus_tag="PGA33_002182"
     CDS             complement(2776..3630)
                     /locus_tag="PGA33_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDG6473983.1"
                     /translation="MNKTNFGAYLNKSKSRKIYKIFDQNLKREFSYWQFRKLFKSYTR
                     QSDVHELKVCTQNDGTIEQIWIDEANQFGVSVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEIFWGGDNELYNYHYLYPHQRYAIDLVIKENHKTYKNQGKVNTDYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTNESQFLGNYIVIKHAENEYSLIAHLHQYSI
                     IVNEGQNVKYGDIIGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFINNRELIKGD
                     VVCGLQAE"
     gene            complement(3841..4656)
                     /locus_tag="PGA33_002183"
     CDS             complement(3841..4656)
                     /locus_tag="PGA33_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HDG6473984.1"
                     /translation="MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVI
                     TAVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEP
                     FLFEDIEDILRHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDSRILEALVRYA
                     NDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMRKYNIPVKSILGFGDS
                     GNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY"
     gene            4950..5375
                     /locus_tag="PGA33_002184"
     CDS             4950..5375
                     /locus_tag="PGA33_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDG6473985.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKEMTVDGY
                     HTVPYTISVDGITALHRTYFIFPENKNVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
BASE COUNT         1999 a          883 c          826 g         1778 t
ORIGIN      
        1 aacattttac atttcatcgt ttttaattac tttaaaaatt gtataactta aatatttaaa
       61 atgatataat cactaagatt gataatattt aattttttga aaattatttt agttgcaatt
      121 tttgggtgat gcggaaaatt tgggtgatcg attgtttata tgcatcaaca ttctttgtgg
      181 attgtttgac agtagacagt catttgtata cgttaacgaa ttttgaaaaa ttgaatcatt
      241 atttaatcat aatattcatt gcgcacgcgc aacacaaaaa ggagacatgt caatgacata
      301 tagaataaag aaatggcaaa aattatccac cattacgtta ttaatggctg gtgtgattac
      361 tttgaatggt ggtgaattca gaagtgttga taaacatcaa atcgctgtgg ctgatacgaa
      421 tgttcaaacg ccagattatg aaaaattgag gaacacatgg ctggacgtta actatggtta
      481 tgataagtat gatgagaata atccagatat gaagaagaag tttgatgcta cagagaaaga
      541 ggcgacgaat ttactcaagg aaatgaaaac tgaaagtggt aggaaatact tgtggtcagg
      601 agcggaaacc cttgaaacta attcttctca tatgactcgt acctatcgta atattgagaa
      661 aatcgcagaa gcgatgagga atcctaaaac cactttaaat actgacgaaa ataagaagaa
      721 agtgaaagat gcccttgagt ggctgcataa aaatgcatat ggaaaagaac cagataaaaa
      781 agtaaaagaa ttaagtgaga attttactaa aacaactggc aagaatacca acttaaattg
      841 gtgggattat gaaattggaa cacctaaatc attaacaaat acgcttatat tgctgaatga
      901 tcaattttca aatgaagaaa agaaaaaatt cactgcccct attaaaactt tcgccccaga
      961 tagtgacaaa atattatctt ctgtaggaaa agctgaactt gctaaaggcg gaaatctagt
     1021 agacatttct aaggtgaaac ttttagaatg tattatcgaa gaagataaag atatgatgaa
     1081 aaagtctata gattcattta ataaagtctt cacttacgtt caagattctg ccactggtaa
     1141 agaacgtaat ggattttata aagatggctc ttacattgat catcaagacg ttccatacac
     1201 tggtgcttat ggcgttgtac tcttagaggg gatttctcaa atgatgccga tgataaaaga
     1261 aacacctttt aatgataaaa cccaaaatga tacaacctta aagtcatgga ttgacgacgg
     1321 atttatgcca ctcatttata aaggtgaaat gatggattta tcacgaggta gagctatcag
     1381 tcgtgaaaat gaaaccagtc actcagcatc tgcaacagta atgaaatcat tgttgagatt
     1441 aagtgatgcc atggatgatt caacaaaagc taagtataaa aagattgtca aatcttcagt
     1501 agagtcagat tcaagttata aacaaaatga ttatttaaat tcatattcgg acatagataa
     1561 aatgaagtct ttaatgacag ataacagtat ttctaaaaac ggattaacac aacaacttaa
     1621 aatatataat gacatggatc gtgtcaccta tcataacaaa gacttagact ttgcatttgg
     1681 tttaagtatg acgtcgaaaa acgtagcacg ctatgaaagt atcaacggag agaatttaaa
     1741 aggttggcac actggtgctg gaatgtctta tttatataac agcgatgtca aacactatca
     1801 tgataacttc tgggtgacag ccgatatgaa acgtttatca ggtacaacaa ctttagacaa
     1861 tgaaatatta aaagatacgg atgataaaaa gtcgagtaaa acttttgttg gcggaacaaa
     1921 agttgatgac caacatgcta gtatcggaat ggattttgaa aatcaggaca aaactttaac
     1981 tgccaaaaaa tcatatttca tattaaacga taaaattgtc ttcttaggaa ctggcattaa
     2041 aagtactgat tcatcaaaga atccagttac aacgattgaa aatcgcaaag cgaatgggta
     2101 tacgttatat acagacgata aacaaacaac caattctgat aatcaggaaa acaattcagt
     2161 ctttttagag tccacagata ccaaaaagaa catcggttat cattttttaa acaaaccgaa
     2221 aataactgta aaaaaagaaa gtcatactgg taagtggaaa gaaataaata aaagtcaaaa
     2281 ggatacacaa aaaactgatg agtattatga agtaactcaa aagcattcta attctgacaa
     2341 taaatatgga tatgtgttgt atccaggctt atctaaagat gtctttaaga caaaaaaaga
     2401 tgaagtaact gtcgttaagc aagaagatga cttccacgtt gtgaaagata atgaatcggt
     2461 ttgggctggt gttaattata gtaatagcac tcaaactttt gacattaaca acactaaagt
     2521 cgaagttaaa gccaaaggta tgtttatact taaaaagaaa gatgataaca cttatgaatg
     2581 tagcttctat aatcctgaat ctacaaattc cgcttcagat attgaatcta aaatttcaat
     2641 gaccggttac tctattacaa acaaaaatac gtcgacttct aatgaatccg gcgtgcactt
     2701 tgaattaact aaataagcaa gaaaatctcg acactatacc atttttatag tgtcgagatt
     2761 ttataagtat cgccatcatt cagcttgtaa tccgcagacc acatcccctt tgataagttc
     2821 tcgattattt ataaatcgaa tttttaaaga tgtacatgct tcaatattct tatcattcat
     2881 tacttgaaaa tgtatatgag gttcagtaga attgccagaa ttcccaacct tcccaatgat
     2941 atcaccatat ttaacatttt gcccctcatt cacaatgatt gaatattggt gtaaatgagc
     3001 tattaagcta tactcatttt ctgcgtgctt aattacaata taatttccta aaaactgtga
     3061 ttcattcgta cttccaatac tgttgtcttg aaccccattc actacttttt caactgtacc
     3121 atttgctggc gcaatgatat cttttccaaa acaaaaataa tcagtattta cttttccttg
     3181 attcttatat gttttatgat tttctttaat aactaaatca atagcatacc tttgatgtgg
     3241 gtataaatag tgataattat ataattcatt atctccaccc caaaaaatct catagcaatc
     3301 tgttgtcggc actgcgtatt ttaaagtcgt ttcattctta atcgcatcat aattaattaa
     3361 cggacgcata acaaatccga taatactatt atctttccaa gtcactgaca ctccaaattg
     3421 atttgcttca tctatccata tttgctcaat agtaccatca ttttgagtac acacttttaa
     3481 ttcatgtaca tcagactgcc ttgtataaga tttgaaaagt ttcctaaact gccaataact
     3541 aaactctcgc ttcaaatttt gatcaaaaat cttatatatt ttcctgctct tacttttatt
     3601 taaataagcc ccaaagtttg ttttattcat atatacttaa cctattatat taattttggt
     3661 atcaattctt tctccagttc ctgcgtcatt tttgcgctac ctgaatcgtc tacacttaat
     3721 tggataaatc aacatataaa taatttttta aagctaaagt gtagtcgatt caccatattt
     3781 tctaatcatc ccttaaatat taatatttgc acttaaaaag tatacatatc actcaactaa
     3841 tcaataatca tacttacctt caacaaattc tttaacatgc agtgttatcc cttgataata
     3901 tggatatttt gttaatctaa atttctgctt taatgcttca tctctactat tcgacatcaa
     3961 atatgcatgt tcaagatagc ttaaatatgc ttcgtcattg ccgctatctc caaaacctaa
     4021 tattgatttt actggtatat tatatttcct catcagaaat tgagtagcat ataattttcc
     4081 agcatttgac ggtgtgaagt caatatcata cgcattttca ggatcaccag ctaatggatt
     4141 gcttcgacta aatctcgctg tatagttttg gtcatttgcg tatctgacaa gtgcttctag
     4201 gatacgacta tcattgttgt gattacctgt tgaatgaaaa taaaaattat aatgtgtttc
     4261 attaccttca aatgctcttt gtggaataaa ttcgacatta tattgcgcag aaatatgacg
     4321 taatatatct tcaatatctt caaataaaaa tggttcgctc aagactttct ttttatatgt
     4381 ttctgattca acatattcgc cattattcca agtgaacatt ttagaactca aatcagaaaa
     4441 gatatgtaat ggtttaaacg tcatattaac ttgatccatt ttatccatga cactttgaaa
     4501 tgtacttcct gtcaatacag cagtgatcac ttcattatta ttagttagtt tttccaacaa
     4561 tttttccatt tctcttaaat gacttaaatc ttcttcattt gtattatgtt taaaatatgt
     4621 ttcatcaaaa tcaaacaata atagacgctt tgacatactc attcccccat aatattttaa
     4681 tgaatttatc tataacttaa atacactata agtctcaatt aaaagtataa gatcgactat
     4741 atgcttttca tgattattat atacaactaa tcaatcccta taaactaaaa acattgctta
     4801 ccacttttca aaatgcacga gtcatatccc tcgttaaaaa ctattactaa aaattaaaat
     4861 ataaaaaatg caaaaagtat gttttaaact ttatgaaacg tgttatacaa atagtgtaat
     4921 atcatacaac ccgaaaagga gattgagata tgaaactaaa atcatttgtt actgccactt
     4981 tagcattggg attattatca acggtcggag ctgcattacc gagtcacgaa gcatctgcag
     5041 atagtaataa cggctataaa gaaatgactg tggatggtta tcacactgtt ccttacacaa
     5101 tttcagtaga tggtattact gcattacatc gaacttactt tatcttccca gaaaataaaa
     5161 atgttcttta tcaagaaatt gacagtaaag taaaaaatga attagcttct caacgtggtg
     5221 ttacaacaga aaaaattaat aatgcccaaa cagcaactta tacgcttact ttgaatgatg
     5281 gtaataaaaa agtagtgaat ctaaagaaaa atgacgacgc taaaaattca attgatccaa
     5341 gtacaatcaa acagatacaa attgtagtta aataataatt tgaaaatagt tttatagggc
     5401 aagcgctgaa aaattggcgt ttgtcttttt agttattgaa cttgtaaatt attgttgttt
     5461 gcttctcgta gtaacagatt ggccta
//
LOCUS       DAMQYT010000165         5423 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMQYT010000165 DAMQYT010000000
VERSION     DAMQYT010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.167"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            10..981
                     /locus_tag="PGA33_002185"
     CDS             10..981
                     /locus_tag="PGA33_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6473986.1"
                     /translation="MKYLLKGNILLLLLILLTIISLFIGVSELSIKDLLHLTESQRNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            968..1924
                     /locus_tag="PGA33_002186"
     CDS             968..1924
                     /locus_tag="PGA33_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDG6473987.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            1921..2682
                     /locus_tag="PGA33_002187"
     CDS             1921..2682
                     /locus_tag="PGA33_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6473988.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFENGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMNIQIEHIRNQRICLYFKD"
     gene            2801..3829
                     /locus_tag="PGA33_002188"
     CDS             2801..3829
                     /locus_tag="PGA33_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDG6473989.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(4146..4460)
                     /locus_tag="PGA33_002189"
     CDS             complement(4146..4460)
                     /locus_tag="PGA33_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473990.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(4478..5401)
                     /locus_tag="PGA33_002190"
     CDS             complement(4478..5401)
                     /locus_tag="PGA33_002190"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDG6473991.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
BASE COUNT         1853 a          773 c          955 g         1842 t
ORIGIN      
        1 aaatttgtaa tgaagtattt attaaaggga aatattttgc ttctattact aatattgttg
       61 acaattattt cgttgttcat aggtgtgagt gaactatcaa ttaaagattt actacattta
      121 actgagtcac agcggaatat tttattctca agccgaatac caaggacgat gagtatttta
      181 attgctggaa gttcgttggc tttagcaggc ttgataatgc aacaaatgat gcaaaataag
      241 tttgttagtc cgactacagc tggaacgatg gaatgggcta aactaggtat tttaattgct
      301 ttattgttct ttccaaccgg tcatatttta ttaaaactag tatttgctgt tatttgcagt
      361 atttgcggta cgtttttatt tgttaaaatc attgatttta taaaagtgaa agatgtcatt
      421 tttgtaccgc ttttaggaat tatgatgggt gggattgttg caagtttcac aaccttcatc
      481 tcattgcgca cgaatgctgt tcaaagcatt ggtaactggc ttaacgggaa ctttgccatt
      541 atcacaagtg gacgctatga aattttatat ttaagtattc ctcttttagc attgacatat
      601 ctttttgcta atcatttcac gattgtagga atgggtaaag actttactaa taatttaggt
      661 ttgagttacg aaaaattaat taacatcgca ttgtttatta ctgcaactat tacagcattg
      721 gtagtggtga ctgttggaac attaccgttc ttaggactag taataccaaa tattatttca
      781 atttatcgag gtgatcattt gaaaaatgct atccctcata cgatgatgtt aggtgccatc
      841 tttgtattat tttctgatat agttggcaga attgttgttt atccatatga aataaatatt
      901 ggtttaacaa taggtgtatt tggaacaatc attttcctta tcttgcttat gaaaggtagg
      961 aaaaattatg cgcaacaata ataaaaaaat aatgctttta attgcagtaa cgttattaat
     1021 tagtatgctg tacttatttg taggtattga ttttgaaata tttgaatatc aattttcaag
     1081 tcgtttaaga aagttcatat taattatttt agtaggtgct gccattgcaa cttcagtggt
     1141 gatttttcaa gcgattacaa ataaccgtct attgacacca tcaataatgg ggttagatgc
     1201 agtttattta tttatcaaag tattgccagt ctttttattt ggaattcaat cggtatgggt
     1261 tactaatgta tatttgaact ttatattaac acttataacg atggtgttat tcgcactaat
     1321 cctattccaa ggtatcttta aaatcggaca tttttcaatt tattttatct tacttattgg
     1381 tgtcctttta ggaacatttt ttagaagcat aacaggtttt attcaactga ttatggatcc
     1441 tgagtcattt ttagcaatac aaagtagtat gtttgctaat tttaatgctt ctaattcgaa
     1501 tttagttact ttctcagcag tgctattagt aatcttatta gtcattacaa ttttactatt
     1561 gccttattta gatgtattgc ttttaggtcg tgctgaagca attaatcttg ggatatcgta
     1621 tgaaaaatta acgcgaattc tacttgtaat agtctcagtt ttagtttctg tgtcaactgc
     1681 attagtagga ccaattacat ttttaggttt attaactgta aatctagcgc atgaactaat
     1741 gaagacgtat gaacataagt atattttaat tgcgacaatt tgcttgagtt ggattagttt
     1801 atttagtgcg caatgggtag ttgaaaatgt gtttgaagct acgacagaaa tgagtatact
     1861 tattgatttg attggtggaa gttatttcat ttatctatta gttagaagga gaaatgcgca
     1921 atgattcaag ttgaaaattt aactaaaact ataaataatc aaatgatatt ggaagatatt
     1981 agcatagata tcgaaaaagg taaattgact tctttaattg gacctaatgg tgcgggtaag
     2041 agtactttac tttcagcgat atgtaggtta attcgttttg agaacggtga agtgaaaata
     2101 gatggacagc tcatgtctga ttataaaaat aatgacttgt cgaaaaaaat atctatatta
     2161 aaacaaacaa accatactga aatgaatatt acggtagagc agttggtaaa ctttggacga
     2221 ttcccttatt ctaaaggtcg tttgacgaaa gaggatcatg atattgtcaa tgatgcgcta
     2281 gatttgttgc aactacaaga tatcagaaat cgtaatatta agtcattatc tggtggacaa
     2341 cgtcagcgtg catacattgc aatgacaata gcacaagata ctgaatatat tttgctagat
     2401 gaaccattaa ataatttaga tatgaagcat gctgttcaaa ttatgcaaac gttaaaaatg
     2461 ttagcgcata aaatgaataa agcgattgtc attgtgttac atgatattaa ctttgcgtcc
     2521 tgttattcag atcagattgt agcattgaaa aacggacaac tagttaagtc agatttgaaa
     2581 gataatgtca ttcaaagtag tgttttaagt gatttatatg acatgaatat tcaaattgaa
     2641 catataagaa atcaaaggat ttgtttatat tttaaggatt gataatttgg agacacttta
     2701 aaggggtgat gcgccaatta aagaagggtt aaacgtaaag catttattta tatttcacat
     2761 caagcacaca gattaagcca aaagaggaga atattatatt atgaagaaaa cagtcttata
     2821 tttagtatta gcagtaatgt ttttattagc ggcatgcggt aacaattctg ataaagaaca
     2881 atcaaaatca gaaactaaag gttctaaaga tacagtaaaa attgaaaata actataaaat
     2941 gcgtggcgag aaaaaagatg gtagtgacgc taaaaaagtt aaagaaactg ttgaagtacc
     3001 aaaaaatcct aaaaatgcag ttgtgttaga ctatggcgca ttagatgtaa tgaaagaaat
     3061 gggcttatca gataaagtaa aagcattacc taaaggggaa ggcggtaagt cattaccgaa
     3121 tttcttagaa tcatttaaag atgataaata tacaaacgtt ggtaatttaa aagaagtgaa
     3181 ttttgataaa attgctgcga cgaaacccga agtaatcttt atctctggac gtacagctaa
     3241 tcaaaagaat ttagatgaat tcaaaaaagc tgcacctaaa gcgaaaattg tttatgttgg
     3301 tgcagatgaa aagaacttaa ttggttcaat gaaacaaaac actgaaaata tcggaaaaat
     3361 ttacgataaa gaagataaag ctaaagaatt aaataaagat ttagataaca aaattgcttc
     3421 aatgaaagat aaaacgaaaa acttcaataa aactgttatg tatttactag ttaacgaagg
     3481 tgaattatca acatttggac ctaaaggtcg ttttggtgga ttagtttacg atacattagg
     3541 attcaatgca gttgataaaa aagtaagtaa tagcaatcat ggacaaaatg tttctaacga
     3601 atatgttaat aaagaaaatc cagatgttat tttagcgatg gatagaggtc aagcgataag
     3661 tggtaaatca actgcgaaac aagcattaaa taatcctgta ttaaaaaatg ttaaagcaat
     3721 taaagaagac aaagtatata atttagatcc taaattatgg tactttgcag ctggatcaac
     3781 tacaactaca attaaacaaa ttgaggaact tgataaagtt gtaaaataat tttaaaagag
     3841 gggaacaatg gttaaaggtc ttaatcattg ctcccctctt ttctttaaaa aaggaaatct
     3901 gggacgtcaa tcaatgtcct agactctaaa atgttctgtt gtcagtcgtt ggttgaatga
     3961 acatgtactt gtaacaagtt catttcaata ctagtgggct ccaaacatag agaaatttga
     4021 ttttcaattt ctactgacaa tgcaagttgg cggggcccaa acatagagaa tttcaaaaag
     4081 gaattctaca gaagtggtgc tttatcatgt ctgacccact ccctataatg ttttgactat
     4141 gttgtttaaa tttcaaaata aatatgatag tgatatttac agcgattgtt aaaccgagat
     4201 tggcaatttg gacaacgctc taccatcata tattcattga ttgttaattc gtgtttgcat
     4261 acaccgcata agattgcttt ttcgttaaat gaaggctcag accaacgctt aatggcgtgc
     4321 ttttcaaact cattatggca cttatagcat ggatagtatt tattacaaca tttaaattta
     4381 atagcaataa tatcttcttc ggtaaaataa tggcgacagc gtgtttcagt atcgattaat
     4441 gaaccataaa ctttaggcat agacaaagct ccttaactta cgattccttt ggatgttcac
     4501 caataatgcg aacttcacga tttaattcaa tgccaaattt ttctttgacg gtcttttgta
     4561 cataatgaat aaggttttca taatctgtag cagttccatt gtctacattt accataaaac
     4621 cagcgtgttt ggttgaaact tcaacgccgc caatacggtg accttgcaaa ttagaatctt
     4681 gtatcaattt acctgcaaaa tgaccaggcg gtctttggaa tacactacca catgaaggat
     4741 actctaaagg ttgtttagat tctctacgtt ctgttaaatc atccatttta gcttgtattt
     4801 cagtcatttt accaggagct aaagtaaatg cagcttctaa tacaactaag tgttcttttt
     4861 gaataatgct attacgataa tctaactcta attcttttgt tgtaagttta attaacgagc
     4921 cttgttcgtt tacgcaaagc gcatagtcta tacaatcttt aacttcgcca ccataagcgc
     4981 cagcattcat atacactgca ccaccaattg aacctggaat accacatgca aattcaaggc
     5041 cagtaagtgc gtaatcacga gcaacacgtg agacatcaat aattgcagcg ccgctaccgg
     5101 ctattatcgc atcatcagat acttcgatat gatctagtga taataaacta attacaatac
     5161 cgcgaatacc accttcacgg ataataatat ttgagccatt tcctaaatat gtaacaggaa
     5221 tctcattttg ataggcatat ttaacaactg cttgtacttc ttcattttta gtaggggtaa
     5281 tgtaaaagtc ggcattacca cctgttttag tataagtgta tcgttttaaa ggttcatcaa
     5341 ctttaatttt ttcatttggg ataagttgtt gtaaagcttg atagatgtct ttatttatca
     5401 cttctcagta catcctttct cat
//
LOCUS       DAMQYT010000166         5379 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMQYT010000166 DAMQYT010000000
VERSION     DAMQYT010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.168"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            102..2447
                     /locus_tag="PGA33_002191"
     CDS             102..2447
                     /locus_tag="PGA33_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HDG6473992.1"
                     /translation="MAWSDFMQKLAQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGY
                     LKEGKRRAGQVMNRSMLTLDIDYAAQDMTDILSMFYDFAYCLYSTHKHREISPRLRLV
                     IPLKRNVNADEYEAIGRKVADIVGMDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLP
                     LLDPDKILNEYVDWTDTLEWPTSSKEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEE
                     AISTFIPDLYEKHSTNRYTYHEGSTAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLV
                     RIHLYGAQDEDAKTDTPVNRLPSYKAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIVN
                     SDDAWSETLEITSKGTFKASIPNIEIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTN
                     FKTRQWQDGDDSSLRSYIEKIYDIHHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGH
                     KRLEKLFIKYLGVEDTEVNRTTTKKALTAGIARVMEPGCKFDYMLTLYGPQGVGKSAL
                     LKKLGGAWFSDSLVSVTGKEAYEALQGVWLMEMAELAATRKAEVEAIKHFISKQVDRF
                     RVAYGHYIEDFPRQCIFIGTTNKVDFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEI
                     DQIWAEAKYYYEQGEELFLNPELEEEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWDD
                     LTIFERRRFYQGDVDMLPTGNVDYVERNKVCALEVFVECFGKDKGDSRGSMEIRKISN
                     ILRQLDNWSVYDGNKSGKIRFGKDYGVQIAYVRDESLEDLI"
     gene            complement(2556..2654)
                     /locus_tag="PGA33_002192"
     CDS             complement(2556..2654)
                     /locus_tag="PGA33_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6473993.1"
                     /translation="MNLMQHWKQTGLNLTARRERGVVSFVASMLHH"
     gene            2788..3078
                     /locus_tag="PGA33_002193"
     CDS             2788..3078
                     /locus_tag="PGA33_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDG6473994.1"
                     /translation="MKESTLEKYLVKEISKLNGLCLKWVAPGTRGVPDRIIIMPEGKT
                     YFVEMKQEKGKLHPLQKYVHRQFENRDHKVYVLWNKEQVNTFIRMVGGTFGD"
     gene            3059..4426
                     /locus_tag="PGA33_002194"
     CDS             3059..4426
                     /locus_tag="PGA33_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDG6473995.1"
                     /translation="MEHLAIDFKPHSYQKYAIDKVIDNEKYGLFLDMGLGKTVSTLTA
                     FSELQLLDTKKMLVIAPKQVAKDTWVDEVDKWNHLNHLKVSLVLGTPKERNDALNTEA
                     DIYVTNKENTKWLCDQYKKEWPFDMVVIDELSTFKSPKSQRFKSIKKKLPLINRFIGL
                     TGTPSPNSLQDLWAQVYLIDRGERLESSFSRYRERYFKPTHQVSEHIFKWELRDGSEE
                     KIYKQIEDICLSMKAKDYLDMPDRVDTKQTVVLSEKERKVYEELEKNYILESEEEGTV
                     VAQNGASLSQKLLQLSNGAVYTDEEDVRLIHDKKLDKLEEIIEESQGQPILLFYNFKH
                     DKERILQRFKEATTLDDSNYKERWNSGDIKLLIAHPASAGHGLNLQQGGHIIVWFGLT
                     WSLELYQQANARLYRQGQNHTTIIHHIMTDNTIDQRVYKALQNKELTQEELMKAIKAR
                     IAKHK"
     gene            4439..4876
                     /locus_tag="PGA33_002195"
     CDS             4439..4876
                     /locus_tag="PGA33_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDG6473996.1"
                     /translation="MGNTIYDIKPGTFKYIESEIYNLNENKKEIKRLRLEILNPTKEQ
                     DSNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNKEKKLKMEQIGHECHMHRNTVTTIRKNFVKAVAYHAGIK"
     gene            5033..5347
                     /locus_tag="PGA33_002196"
     CDS             5033..5347
                     /locus_tag="PGA33_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDG6473997.1"
                     /translation="MTKHNNIYKHGRKSYQYDWFYHSKAWKKLREIALDRDNHLCQMC
                     LREDIVTDANIVHHIIYVDEDFNKALELDNLMSVCYGCHNKIHANGNDKSNLKKIRVL
                     KI"
BASE COUNT         2062 a          727 c         1066 g         1524 t
ORIGIN      
        1 tgttagacaa agtcactcaa atagaaacaa ttaaatatga tcgtgatgtc tcatattctt
       61 atgctgctag tcgtttatcc acatattgga ctaatcacaa tatggcttgg tctgacttta
      121 tgcagaagct agcacaaaca gttagaacta aagaagattt aactgagtac aataaaatgt
      181 ctaagtctga acaagcagat ataaaagatg ttggcggatt tgtcggtgga tatttaaaag
      241 aaggcaaacg acgtgctggt caagtcatga atcgttcaat gctaacactt gatatcgatt
      301 atgctgctca agatatgact gacatattat ctatgtttta tgattttgca tattgtttat
      361 attcaacaca taagcataga gagataagtc caagactgcg tttagtgatt cctttaaaac
      421 gaaatgtaaa tgcagatgag tatgaagcta ttgggcgtaa agtcgcagat atcgttggca
      481 tggattactt cgatgataca acttatcaac cacataggtt aatgtattgg ccttcaacta
      541 gcaatgatgc agaatttttc tttacctatg aagatttacc tttgttagat ccagataaaa
      601 tattaaatga atatgttgat tggactgaca cattagaatg gccaacgtct tcaaaggaag
      661 agagtaagac taaaagatta gcagataagc aaggtgaccc agaagaaaag ccgggaattg
      721 ttggcgcatt ttgtagagcc tatacgatag aagaagctat atcaactttt attcctgact
      781 tatacgaaaa acattctact aaccgttata cctatcatga aggttcaact gcaggtggat
      841 tggtgttata cgaaaataac aagtttgcct attctcatca taatacggat cccgttagcg
      901 gtatgcttgt gaacagtttt gatttagtac gcatacactt atatggtgct caagatgaag
      961 acgctaaaac agatactccg gttaatcgac tacctagtta taaagcaatg cagcaaagag
     1021 cgcaaaatga tgaagttgtt aaaaagcaat taattaacga caaaatgtct gatgcaatgc
     1081 aggatttcga tgaaatagta aatagcgatg atgcatggtc tgagacgtta gaaattactt
     1141 cgaaaggtac tttcaaagct agtatcccaa atatagaaat tatattgcgt aatgatccaa
     1201 atttaaaagg aaaaatagca tttaatgaat ttacaaaaca aattgaatgc ttagggaaag
     1261 tgccatggaa tactaatttt aagacacgtc aatggcaaga cggtgatgat agcagtttaa
     1321 gaagttatat cgaaaagatt tatgacatac accattcagg taaaacaaaa gatgccatta
     1381 taagcgtagc aatgcaaaat gcttatcatc cagtaaggga ttatctaaat aaaatatcgt
     1441 gggatggaca taaacgtctt gaaaagttat ttatcaaata cttaggtgtt gaagatactg
     1501 aagtgaatag aacaactacc aaaaaagcat tgactgctgg aattgctcga gtaatggagc
     1561 ctggatgtaa atttgactat atgcttacac tttatggtcc tcaaggtgta ggtaaatctg
     1621 ctttgctaaa aaaattaggt ggtgcatggt tttctgacag tttagtttct gttacaggta
     1681 aagaagccta tgaggcctta caaggcgttt ggctaatgga aatggcagaa cttgcagcta
     1741 caagaaaagc tgaagttgaa gctattaagc atttcatatc taaacaagtt gaccggtttc
     1801 gtgttgctta tggacattat attgaagatt ttccaaggca atgtattttc attggtacaa
     1861 ctaataaagt tgatttctta agagatgaaa ctggtggaag acgtttttgg ccaatgactg
     1921 taaatccaga gagagttgaa gtgaactggt ctaaactaac caaagaagag atcgaccaaa
     1981 tttgggcaga agctaaatat tattatgaac aaggagaaga gttattcctc aaccctgaac
     2041 tagaagaaga aatgcgttca atacaaagca aacatactga ggaatctcca tatacaggca
     2101 ttattgatga atatcttaac acaccaattc ctagcaattg ggatgactta actatctttg
     2161 aacgaagacg attttatcaa ggtgatgttg atatgttacc aacaggaaat gtagattacg
     2221 ttgaaagaaa taaggtctgt gcgcttgaag tgtttgttga atgttttggt aaagataagg
     2281 gagatagtag aggatctatg gaaattagaa agatttcaaa catcttaaga caattagaca
     2341 attggtctgt atatgatggt aataaaagtg ggaaaattcg atttggaaaa gattatggtg
     2401 tacagatagc ttatgtaaga gatgaaagtt tagaagattt aatataataa atattgaata
     2461 aatatacatt ttagagtgtt gtatcagatg ttgcatcatt ttttgagtga tgcaacacgt
     2521 gagtgtaaaa agtaatcgta ggtgttgcat catttttagt gatgcaacat tgatgcaaca
     2581 aatgatacaa cacctctttc ccttctcgct gtaaggttca accctgtttg tttccaatgt
     2641 tgcatcaaat tcactataaa gtttaaaaag tagtgttagg gagtaaagag gtataggggt
     2701 aaccctctaa cagctatttt taaaagtttg gcaagaattg atgcaacatc ggaacacaaa
     2761 tataaatttt gtatacaagg tgaatatatg aaagaatcga cattagaaaa atatttagtg
     2821 aaagagatat caaagctaaa cggtttatgt ttaaaatggg ttgcacctgg aacaagaggt
     2881 gtgccagata gaattattat tatgccagaa ggaaaaacat attttgtaga aatgaagcaa
     2941 gaaaaaggaa agttgcatcc tttacaaaaa tatgtgcata gacaatttga aaatagagat
     3001 cataaagtat atgtgttatg gaataaagaa caagtaaata cttttatcag aatggtaggt
     3061 ggaacatttg gcgattgact tcaaaccaca tagctatcaa aagtatgcaa tagataaagt
     3121 gatagataat gagaaatacg gtctgttttt agatatgggt ctagggaaaa cagtatcaac
     3181 acttacagca tttagtgaat tgcagttgtt agacactaaa aaaatgttag ttatagcacc
     3241 taaacaagtt gctaaagata catgggttga tgaagttgat aagtggaacc atttaaatca
     3301 tctgaaagtg tctttagttt taggaacacc taaagaaaga aatgatgcat taaacacaga
     3361 ggctgatatc tatgtaacca ataaagaaaa tactaaatgg ttatgtgatc aatataaaaa
     3421 agaatggcca tttgacatgg ttgtgattga tgaactgtct acatttaaaa gtcctaagag
     3481 tcaaaggttt aaatctatta aaaagaaatt accactcatt aatagattta taggattaac
     3541 aggaacacct agtccaaata gtttacagga tttatgggct caagtttatt tgatagacag
     3601 aggtgaaaga cttgagtctt cattcagtcg ttatcgagaa aggtacttta aaccaactca
     3661 tcaagttagc gaacatattt ttaagtggga gctaagagac ggatctgaag aaaagatata
     3721 taaacaaata gaagatatat gtttaagcat gaaagcgaaa gattatctgg atatgcctga
     3781 cagagttgat actaaacaaa cagtagtctt atcagaaaaa gaaagaaaag tatatgaaga
     3841 attagaaaaa aactatattt tagaatcgga agaagaagga acagttgtag ctcaaaatgg
     3901 ggcatcatta agtcagaaac tacttcaact atctaacggc gcagtttata cagatgagga
     3961 agatgtaaga cttatacatg ataagaagtt agataagtta gaggaaatta tagaggagtc
     4021 tcaaggccaa ccaatactat tgttttataa cttcaaacac gataaagaaa gaatacttca
     4081 aaggtttaag gaagcaacca cattagacga ttcaaactat aaagaacgtt ggaacagtgg
     4141 agacattaag ttgcttatag cacatccagc aagtgcagga catggattaa acttacaaca
     4201 aggtgggcac attattgttt ggtttggact tacatggtcc ttggaattat accaacaagc
     4261 aaatgctaga ttatacagac aaggacaaaa tcatacgact attattcatc atatcatgac
     4321 cgataacaca atagatcaaa gagtatataa agctttacaa aataaagaac taacgcaaga
     4381 agaattaatg aaagctatta aagcaagaat agctaagcat aagtaatgga ggtctaacat
     4441 ggggaacaca atatatgata tcaagccagg cacatttaaa tatattgaat cagaaatata
     4501 taatttaaat gagaacaaga aagaaataaa aagattgagg ttggaaatac ttaatccaac
     4561 gaaggaacaa gattccaata ttgtatatgg accattacaa aaaggcgaac cagttagaac
     4621 aactgaacta atggcaacta gattattaac taataagatg ttacgaaacc ttgaagaaat
     4681 ggtcgaagca gttgagagtg aatacttaaa gttgcctgaa gatcataaga aagtaataag
     4741 gctcaagtat tggaataaag aaaagaagtt aaagatggaa cagataggac atgaatgcca
     4801 tatgcatcgt aatactgtta ctactatacg aaagaacttt gttaaagcgg tagcgtatca
     4861 tgcaggtatc aaataacatt gtgcaaagat tgtgcaaaag gcctacaaat ctgtagtaat
     4921 atgatagtat cggaaagatg tataaagtta tctaaaagtt atacgacaca agtacacgag
     4981 gcacatcgct atgcggtgtg tcttttgtta tgcaatcaaa gaggtgtaag agatgaccaa
     5041 gcacaataac atttataagc atggtcgtaa gtcatatcaa tacgattggt tctatcattc
     5101 aaaagcatgg aagaagttaa gggagatagc attagataga gataatcatc tttgtcaaat
     5161 gtgtttacgc gaagatattg taacagatgc aaacatagtg catcacatta tttatgttga
     5221 tgaagatttt aacaaagctt tagaattaga taatctaatg tcagtttgtt atggctgtca
     5281 taacaaaatt catgcaaatg gtaatgacaa aagtaatctt aagaaaatta gagttctaaa
     5341 aatttaaata aaaaaacatt taaataaaat tttatagcc
//
LOCUS       DAMQYT010000167         5350 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMQYT010000167 DAMQYT010000000
VERSION     DAMQYT010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.170"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            123..1241
                     /gene="ald"
                     /locus_tag="PGA33_002197"
     CDS             123..1241
                     /gene="ald"
                     /locus_tag="PGA33_002197"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDG6473998.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
     gene            1336..2376
                     /gene="tdcB"
                     /locus_tag="PGA33_002198"
     CDS             1336..2376
                     /gene="tdcB"
                     /locus_tag="PGA33_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDG6473999.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            2407..3729
                     /locus_tag="PGA33_002199"
     CDS             2407..3729
                     /locus_tag="PGA33_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDG6474000.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            3886..5277
                     /gene="norB"
                     /locus_tag="PGA33_002200"
     CDS             3886..5277
                     /gene="norB"
                     /locus_tag="PGA33_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HDG6474001.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEIFYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
BASE COUNT         1865 a          758 c         1019 g         1708 t
ORIGIN      
        1 ttaaatattt attaacaaat tatagataaa atatgaataa ttaattaatg gtatttacat
       61 attcataacc aaaggataaa ataattgtga aattattcac aaatgaaaga ggagtgttgc
      121 aaatgttagt tgcagtagtc aaagaattaa aacaaggtga aggacgtgta gcttgcacac
      181 ccgaaaatgt gcgtaagtta acggatgcag gacataaagt aattgttgaa aaaaatgctg
      241 gcattggttc aggattttct aacgatatgt atgaaaaaga aggcgctaag atcgtaactc
      301 acgaacaagc atgggaagct gatcttgtta tcaaagtaaa agaacctcat gaaagcgaat
      361 atcaatattt caaaaagaat caaattatct ggggattttt acatctagca tcttcaaaag
      421 aaatagtaga aaaaatgcaa gaagttggtg taactgcgat tagtggtgaa accattataa
      481 aaaatggaaa agcagaatta ttagcgccaa tgagtgctat agcaggtcaa cgctcagcaa
      541 ttatgggagc ttactactct gaagcacaac atggtggtca aggtacttta gtgactggtg
      601 tacatgaaaa tgtggatata cctggtagta catatgtgat tttcggtggt ggagtagcag
      661 caacaaatgc agcaaatgtt gccttgggac taaatgctaa agtaatcatt atcgagttaa
      721 acgatgaccg cattaaatat cttgaagata tgtatgcaga aaaagatgtc acagtagtca
      781 aatcaacacc agaaaattta gcagaacaaa ttaagaaagc agatgtattt atttctacaa
      841 ttttaattcc aggtgcgaaa ccgccaaaat tggttactcg tgagatggtt aaatcaatga
      901 aaaaaggttc agtattaatc gatatagcta ttgaccaagg tggaactatt gaaacaatta
      961 gaccaactac aatttctgat ccagtgtatg aagaagaagg tgtgattcat tatggtgtac
     1021 caaatcaacc aggagcagtc ccaagaactt caacaatggc attagcacaa ggaaatattg
     1081 attatatatt agaaatttgt gacaaaggct tagaacaagc aattaaagat aatgaagcct
     1141 taagtactgg tgtaaacatt taccaaggac aagtgacaaa tcaaggatta gcttcatcac
     1201 atgacctaga ttataaagaa atattaaatg ttatcgaata gatagtaatt taaatgaaat
     1261 tgagtgaaat gaatatttta aatatagcat tatagtttgg actaaaaatt tacaaaacgg
     1321 aaggatgtaa ttattatgac aaccaacaca gttacattac aaacagcaca tattgtaagt
     1381 ttaggagata tcgaagaagc taaagcaagc attaaaccat ttattcgtcg aacacctcta
     1441 attaaatcaa tgtatttaag ccaaagtata actaaaggga atgtatttct aaaattagaa
     1501 aatatgcaat tcacaggatc ttttaaattt agaggcgcta gcaataaaat taatcactta
     1561 acagatgaac aaaaagaaaa aggcattatc gcagcatctg ctgggaacca tgcacaaggt
     1621 gttgctttaa cagctaaatt attaggcatt gatgcaacga ttgtaatgcc tgaaacagca
     1681 ccacaagcga aacaacaagc aacaaaaggc tatggggcaa aggttatttt aaaaggtaaa
     1741 aactttaacg aaactagact ttatatggaa gaattagcga aagaaaatgg catgacaatc
     1801 gttcatccat atgacgataa gtttgtaatg gcaggccaag gaacaattgg tttagaaatt
     1861 ttagatgata tttggaatgt gaatacagtc atcgtaccag ttggcggtgg aggattaatt
     1921 gcaggtattg ccaccgcatt aaaatcattt aacccttcaa ttcatattat cggtgttcaa
     1981 tctgagaatg ttcatggtat ggctgagtct ttctataaga gagatttaac tgaacatcga
     2041 gtggatagca caatagcaga tggttgtgat gtaaaagttc ctggtgaaca aacatatgaa
     2101 gtagttaaac atttagtaga tgaatttatt cttgttactg aagaagaaat tgaacatgct
     2161 atgaaagatt taatgcagcg tgccaaaatt attactgaag gtgcaggcgc attaccaaca
     2221 gctgcaattt taagtggaaa aataaacaat aaatggcttg aagataaaaa tgttgttgca
     2281 ttagtttcag gcgggaatgt tgacttaact agagtttcag gtgtcattga acatggactg
     2341 aatattgcag atacaagcaa gggtgtggta ggttaaaaca tttaatctta aaaatgaggt
     2401 gtaattatgt caaatggtaa agaattacaa aaaaatatag gtttcttctc agcgtttgct
     2461 attgttatgg ggacagttat tggttcagga gtattcttta aaatatcaaa cgtaacagaa
     2521 gtaacaggaa cagcaggaat ggccttgttt gtatggttcc taggcggcat cattaccatt
     2581 tgtgcggggt taacagcagc agaacttgct gctgcaatcc ctgaaacagg tggcttaacg
     2641 aagtatatag aatatacata cggtgatttc tggggcttcc tatcaggttg ggcgcaatca
     2701 tttatttatt ttccagctaa cgtagcagca ttgtctatcg tatttgcgac acagctaatt
     2761 aatttattcc atttatctat aggttcgtta ataccaatag caatcgcatc tgcgttatct
     2821 attgtgttga taaatttcct aggttcaaaa gcaggcggaa ttttacaatc agttacttta
     2881 gtaattaaac tgattccaat catcgttatt gtaatttttg gtatttttca atctggagat
     2941 atcacttttt cattaattcc aactacaggt aattcaggaa atggcttctt tacagcaatt
     3001 ggtagtggtt tattagcaac tatgtttgca tatgatggtt ggattcatgt aggaaatgtt
     3061 gcgggggaac ttaaaaatcc taaacgcgat ttacctttag cgatttcagt tggtatcggt
     3121 tgtattatgg ctgtgtattt attaattaac gctacattct tattaacgtt gccaatagaa
     3181 ctacttgctg gtaatttaaa tgcagcttca gatacatcaa aaatattatt tggtgaaaat
     3241 ggcggtaaga ttattacaat cggtatatta atttctgttt atggtacgat caatggctat
     3301 actatgactg gtatgcgcgt accatatgca atggctgaaa gaaaattatt gccatttagc
     3361 catttattcg caaaattaac aaaatctggc gcaccatggt ttggcgcaat tatacaactt
     3421 ataatcgcta tcatcatgat gtcaatggga gcatttgata caattacaaa tatgttaatc
     3481 tttgttattt ggttgttcta ttgtatgtca tttgttgcgg taataatttt aagaaaacgt
     3541 gaaccaaata tggaacgacc atataaagta ccgttatatc cgatcatacc tttaattgct
     3601 attttggcag gatcatttgt attaattaat acactgttta cacaatttat attagcaatc
     3661 attggaattc taataacagc acttggtata ccagtttatt actataaaaa gaaacaaaaa
     3721 gcagcataag gtaagataac tagcattgag aataaatgga tggactacta ataaatttaa
     3781 agttttacac attaaaatca aaaaccattc aattattcta tggaacagac aaatttctgt
     3841 tatggaattt gtctgttttt caaaagtata gggaggcaaa tagagatgga aaagccgtca
     3901 agagaggcat ttgaaggcaa taataagttg ttaataggaa ttgttctaag tgtaataacg
     3961 ttttggctat ttgcacaatc attggttaat gttgtaccaa tacttgaaga tagtttcaat
     4021 acagatattg gaacggttaa tatcgccgtt agtataactg ctttattttc aggaatgttt
     4081 gtagtaggag caggtggtct tgctgataaa tatggcagaa ttaaactcac gaacattggt
     4141 attatcttaa atatattagg ttcattatta atcattattt caaatattcc tttattactt
     4201 attataggaa gattaattca aggactttca gcagcatgta ttatgcctgc aactttgtct
     4261 attattaagt catattacat tgggaaagat agacaacgcg ctttaagtta ttggtcaatt
     4321 ggctcatggg gcggctctgg tgtttgttca ttttttggag gtgcagttgc aacgctttta
     4381 ggttggcgtt ggattttcat cctatcaatt ataatttcat taattgcact gtttcttatt
     4441 aaaggcacac ctgaaactaa atctaaatcg atttctctaa ataaatttga cattaaaggt
     4501 ctggttcttt tagtcattat gctcctcagt ttaaatattt taattactaa aggatcagaa
     4561 ttaggtgtaa cctcacttct ttttattact ttattagcta ttgcaattgg atcttttagt
     4621 ttatttatag ttcttgaaaa gcgtgctaca aatcctttaa tcgattttaa attatttaaa
     4681 aataaagctt acacaggtgc aacagcttca aactttttgt taaatggtgt tgcaggaaca
     4741 ttaatagtag ccaacacatt tgttcaaaga ggtttaggat attcttcatt gcaagcagga
     4801 agtttatcaa tcacttattt agtaatggta ctaattatga ttcgtgttgg tgaaaagtta
     4861 cttcaaacac tcggatgcaa gaaaccaatg ttaattggaa caggagttct tattgtcgga
     4921 gaatgtctca tttcattaac tttcttgcca gaaatattct atgtcatttg ttgtattata
     4981 ggttatttat tctttggttt aggactaggg atatatgcta caccatcaac agatacagca
     5041 attgcaaatg caccgttaga aaaagtaggc gttgctgcag gtatctataa aatggcttct
     5101 gcattaggtg gagcatttgg cgtcgcattg agtggtgcag tatatgcaat cgtatcaaat
     5161 atgacaaaca tttatacagg tgcaatgatt gcattatggt taaatgcagg tatgggaata
     5221 ttatcattcg ttatcatttt gttacttgtg cctaaacaaa acgatactca attatgataa
     5281 ttgagaatta aattgaaatc atacaagtcg ctacaatatt aaacaaaaat ataaaccgat
     5341 tcttatgtgt
//
LOCUS       DAMQYT010000168         5316 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMQYT010000168 DAMQYT010000000
VERSION     DAMQYT010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.171"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            35..808
                     /locus_tag="PGA33_002201"
     CDS             35..808
                     /locus_tag="PGA33_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDG6474002.1"
                     /translation="MIHSKRLRLWLYLVLLAVFIGACGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDQEFEKGDKGTWIIHSKMTIETNGKNMESRGLVLYVDRNT
                     RTTKGEFIVRELWEDKKGYSRSKEKEYPVKMEHNKIIPTKPIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNDDYNVKQLRKRYDIPTKKAPKLLIK
                     GDGDLKGSSIGHKNLEFTFIENKEENIYFTDSINFKPTE"
     gene            920..1704
                     /locus_tag="PGA33_002202"
                     /pseudo
     CDS             920..1704
                     /locus_tag="PGA33_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            1701..3025
                     /locus_tag="PGA33_002203"
                     /pseudo
     CDS             1701..3025
                     /locus_tag="PGA33_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3025..3351
                     /locus_tag="PGA33_002204"
     CDS             3025..3351
                     /locus_tag="PGA33_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474003.1"
                     /translation="MFTTYKNINELENAYDEERKQLNDAFNQIDELRHQTRKKCEQMY
                     DHFLYLKHKMNYSEDAMIRMTRIIESFDRETNQRIRHHEMKLEDYKDELRREYLKQSD
                     RIEGDE"
     gene            3354..3641
                     /locus_tag="PGA33_002205"
     CDS             3354..3641
                     /locus_tag="PGA33_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474004.1"
                     /translation="MGLDFSGLPDLAVLEQMKEKEQISEVIAPEHVRMHHDHQNKLKS
                     DEKILLDQMVSHFKKFEDDFKNAAQGAWVKNATDELKDISNDLEKIQDIKV"
     gene            3958..4065
                     /locus_tag="PGA33_002206"
                     /pseudo
     CDS             3958..4065
                     /locus_tag="PGA33_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
     gene            4177..4971
                     /locus_tag="PGA33_002207"
     CDS             4177..4971
                     /locus_tag="PGA33_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDG6474005.1"
                     /translation="MIHSKKLTLGICLVLLIILIVGYVIMTKTNGRNAQIKDTFNQTL
                     KLYPTKNLDDFYDKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNT
                     RTTKGYYFISEMTDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLKKEIENFKFFV
                     QYGNFKDINDYKDGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDIPTKQAPKLLLK
                     GDGDLKGSSVGSRSLEFTFVENKEENIYFTDSVQYTPSEDTRYESN"
     gene            complement(4975..5097)
                     /locus_tag="PGA33_002208"
     CDS             complement(4975..5097)
                     /locus_tag="PGA33_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474006.1"
                     /translation="MSYIIKFTFKFFEMATYISQLTLDIELVSSELLVILPGFI"
BASE COUNT         2185 a          674 c          969 g         1488 t
ORIGIN      
        1 tttgcatcaa aaataaaaat ggggatgtga aatgatgatt cactcaaaaa ggttgaggct
       61 ctggctgtat ttagtattat tagctgtttt tataggggct tgcggaatga aaaaggaaga
      121 aagcagtaaa gataaacaaa ttaaagaaaa cttcaacaaa acgttgagcc tgtatccaac
      181 taaaaatctt gaagactttt atgataagga aggctttcga gatcaagagt ttgaaaaggg
      241 cgacaaaggc acttggataa ttcactctaa aatgacaata gaaacaaatg gaaagaatat
      301 ggaatccaga ggattggtcc tgtatgtaga ccgcaatacc agaacgacaa aaggtgaatt
      361 tattgtgagg gaattatggg aagataaaaa aggatactca cgtagtaaag aaaaagaata
      421 tccagtaaaa atggaacata ataaaattat cccaaccaaa ccgatagctg atgataaatt
      481 aagaaaagaa atcgaaaact ttaaattctt tgtacaatac ggtgacttta aagatattaa
      541 tgattataaa gatggtgaca tttcatataa tccgaatgtg ccaagttatt cagcaaagta
      601 tcaattaagt aatgatgact acaatgtgaa gcaacttaga aagaggtatg atataccaac
      661 taagaaagca cctaaattat taataaaagg cgatggtgat ttaaaaggat catctatagg
      721 acacaaaaat ttagagttta catttataga aaataaagaa gaaaacatct attttacgga
      781 tagtattaat ttcaaaccta ctgaataggg ttaatagaag tttgaaatga aaatttcata
      841 aagtgttcaa aataattttt cgctttagtt gtgtaaatta aagattgagt cagtattaaa
      901 aatataaggt ggagaaatga tgattcattc aaagaaatta acgctcggta tatgtttggt
      961 attactcatt atattgattg gaggttgtgt cattatgaca aaaacaaatg gtcgaaacgc
     1021 tcaaattaaa gaaaatttca acaaaacatt aagtgtatat ccaaccaaga atctcgatga
     1081 tttttacgat aaggaaggtt ttcgagatca agaatttgat aaaagagata aagggacttg
     1141 gattatttat tctgaaatgg ttatcgaacc aaaagggaaa aatatggaat cgagaggaat
     1201 ggtgctctat atcaatcgca atactagaac aacgaagggt aattttattg tcaccgaaat
     1261 aactgaagat agtaaaggat attcacgtag taaagaaaaa aatatcctgt caagatggaa
     1321 aataatcgaa ttattccaac aaagcctata ccggatgaca agttaaaaaa agagattgaa
     1381 aactttaagt tctttgtaca atatggaaat tttaaagatt ttaaagatta taaaaatggt
     1441 gatatttcat ataatcctaa tgtgccaagt tattctgcaa agtatcaatt gaataatgat
     1501 gactataatg ttcaacagtt aagaaaacga tatcatattc caaccaaaca agcgcccgaa
     1561 ttaaaattga aaggatccgg caatttaaaa ggctcatccg taggatctaa ggatctagaa
     1621 tttacgtttg tagaaaatca agaagagaat atctattttt cagattcggt cgaatttaca
     1681 cctagcgagg atgataaatc atgagtcaaa cggaatatca aataaaatct ggcaatataa
     1741 aaggtaactc tgaagaaaca agtacagtat ctaatataag ttatgaaata gaaaacgcaa
     1801 ataacagtgg tttaaaacaa aataaaattg ataaacaaat taaaaagtta caagaaaaaa
     1861 ataaattccc taaaaatctt tcatatctta aaagttatac ggaccccaaa acaggcacga
     1921 ctacaagcgc ctttttaaat aaagacactg gcaaagttac tttaggtatg acaggtacta
     1981 atgtacacaa agacgcaata ttaaaacaaa catttggtgt tccttcttat caaggatata
     2041 tagatgtgag tgaaacgcta aaggatattg gggccgatgt caatattggc cttcattccg
     2101 tcacagataa agatccacat tataaaaata cccaagactt tatcaaaaat atcaaaaaag
     2161 actatgatat tgatattatt accggacatt cgctgggcgg tagagatgcg atgattttag
     2221 gtatgagtaa tgatattaaa catatcgttg tgtataatcc agctccatta gcgatcaaag
     2281 atgtgagcgg cctttatgcc gatcaagaag aacttaaaaa attgattgaa aagtacgatg
     2341 gtcacattgt aagatttgtg tctgatgaag acgaattaga tgcaggtgtc cgcaatcatt
     2401 tatatgaaac tgctggagaa aaaatagtac ttaaaaatgg agaaggccat gcaatgagtg
     2461 gtattttaat gagcagaaca caggctataa tcttagctga attaaacaaa gttaaaggct
     2521 accaagacga aaataataaa gcattaaaat ccgttcgtaa acaaacgagg catagattac
     2581 ataaagtaga gacgttaaga gcgaattgga ttcaaacaac gggtggatca ctctcttcct
     2641 cccaacaaca attattagaa gctttaacag cactaaccat tgccgaaggc ttaaatcaat
     2701 tagtgaatga agaaagccaa catttgaaaa aatgtatcac gcgatggcac ataaatttgg
     2761 agacaactgg aaaaaagcgc aagaagttgg aaatgaaatt ggtgaaaaat taacctctga
     2821 agaggttata gatgaattaa gaaaaggtgg cgcgtatgaa agtaaacttg aaacagatcc
     2881 caaaagaaaa attgatgata agataaagaa attaaatgat gtttataaaa attgtaatgg
     2941 ctatatcgca aaaattaaac agagtatcga agcaattgtt tctaatgacc aaatgttagc
     3001 gagccagatt gatgggatga tgtaatgttt actacgtata aaaatattaa tgaacttgaa
     3061 aatgcctatg atgaagaaag aaaacaattg aatgatgcat tcaatcaaat tgatgaatta
     3121 agacatcaaa cacgcaagaa atgtgaacaa atgtatgatc atttcttata tctcaaacat
     3181 aaaatgaatt atagtgaaga cgctatgatc aggatgacac gtattataga atctttcgat
     3241 agagaaacga atcaacgtat ccgacatcac gaaatgaaat tagaagatta taaagatgag
     3301 ttaagaagag aatatctaaa acaatctgac agaattgaag gagatgaata agcgtgggac
     3361 ttgattttag tggtttacca gatttagcag tattggaaca aatgaaggaa aaagaacaga
     3421 ttagtgaggt tattgcgcct gaacatgttc gtatgcatca tgatcatcaa aataagctga
     3481 aaagtgatga gaaaatatta cttgaccaaa tggtaagtca tttcaaaaaa tttgaagatg
     3541 attttaaaaa tgcggcacaa ggggcttggg tgaaaaatgc cacagacgaa ttaaaagata
     3601 ttagtaatga tttagaaaaa attcaagata ttaaagtata aaaaggtatt aagaaacaca
     3661 ataagtatat aatccattta ataataaatg aattatatag ttcataattt cgactataag
     3721 tggctattag ccattatttt tcgggatcta tgtcaaatcg gactaatgaa ttcaataatg
     3781 gaagttaagc aaccaatctt tgtttaactt cttttttatt ttttggaaaa taaagttttg
     3841 aacataataa aatttgatta tgttttaacg aattttgatg tttcttgaac tatatatcat
     3901 ctagtcgtca tttacaattg gtaaatatga cttcaaactg tatgaaggtg atggcgatta
     3961 aaaggctcat ccgtaggttc taaagaacta gaatttaact ttataagaaa taaagaagaa
     4021 aatgtgtatt tttctgatag tattaatttc aaacctactg aatagggtta atagaagttt
     4081 gaaataaaaa tttcataaag tgttcaaaat aatttttcgc tttagttgta aatattaaaa
     4141 actgagtcaa tattaaaaat ataaggtgga gaaatgatga ttcattcaaa gaaattaacg
     4201 ctcggtatat gcttggtatt actcattata ttgattgtag gttatgtcat tatgacaaaa
     4261 acaaatggtc gaaacgccca aattaaagac acatttaatc aaacattaaa attatatcca
     4321 accaaaaatc tcgatgattt ttacgataaa gaaggctttc gagatcaaga atttaaaaag
     4381 ggtgataaag gtacttggat agttaattct gaaatggtaa tcgagccaaa aggtaaggat
     4441 atggaaacga gaggaatggt gctctatatc aatcgcaata ctagaaccac aaaagggtat
     4501 tattttataa gtgaaatgac agatgacagt aacggcagac caaaggatga tgaaaaaagg
     4561 tatccggtaa aaatggaaca taataaaatc ataccaacga agccactacc gaatgacaag
     4621 ttaaaaaaag agattgaaaa ctttaagttc tttgtacaat atggcaactt taaagatatt
     4681 aatgattata aagatggtga tatttcatat aatcctaatg taccaagtta ttcggcaaaa
     4741 tatcaattga ataatgatga ttataatgtc caacagttaa gaaaaagata tgatattcca
     4801 accaaacaag cgccgaaact attattgaaa ggcgatggag atttaaaagg ttcatccgta
     4861 ggttctagaa gtcttgaatt tacctttgtc gaaaataaag aagaaaacat ttattttaca
     4921 gattctgtac aatatactcc aagtgaggat acaaggtatg agtcaaactg aatatcaaat
     4981 aaaacctggt aatataacaa gtaactctga agaaacaagt tcgatatcta aagtgagctg
     5041 tgaaatatag gtagccattt caaaaaattt aaaggtgaat ttgataatgt agctcaagga
     5101 gattgggtta aaaaggcgaa gaatgaactg gatgatatta gtaagaaatt aaaaaatatt
     5161 caaagaacgg aagtttaata gcttatatga ttcttggagc taagacagca tgcgttcatt
     5221 catgccatta ttaatataag caccgcaaca aaaaagcttc taatgtgata caggaacctc
     5281 atattccgta tcatgttaga agcttttaat gtctaa
//
LOCUS       DAMQYT010000169         5285 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMQYT010000169 DAMQYT010000000
VERSION     DAMQYT010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.172"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(11..1603)
                     /gene="pckA"
                     /locus_tag="PGA33_002209"
     CDS             complement(11..1603)
                     /gene="pckA"
                     /locus_tag="PGA33_002209"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDG6474007.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     regulatory      1758..1868
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1974..3167
                     /locus_tag="PGA33_002210"
     CDS             1974..3167
                     /locus_tag="PGA33_002210"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDG6474008.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     gene            3292..4200
                     /locus_tag="PGA33_002211"
     CDS             3292..4200
                     /locus_tag="PGA33_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HDG6474009.1"
                     /translation="MDLSSPIVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNLKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            4412..5245
                     /locus_tag="PGA33_002212"
     CDS             4412..5245
                     /locus_tag="PGA33_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDG6474010.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNNVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQRIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHAVVTIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKKFE
                     G"
BASE COUNT         1800 a          810 c          979 g         1696 t
ORIGIN      
        1 gtattcaaat ttatttgttg aagctacctt tttcagcaat atgttcaact ttttcaccaa
       61 atttttcgaa gttcttttca aaacgttgaa ttaaatcttc tgcttgtgct ttatatttct
      121 ctttgtcgct ccaagcatta attggattta aaattgtttt cggtacatct tcaatttcta
      181 caggaatgct taaaccaaac gtactatctt ttgtatattc tgcatttttc aatttgccag
      241 aaatcgcttg gtttaccatt tgacgtgtgt aatgtaagct gattctacgt cctacaccat
      301 attttccgcc agtccatcca gtattaacaa gataaacatc aacatcatgt aaatcgataa
      361 gttcacctaa tagatcagcg taaacagtag ggtgtaacgg gaagaacggt gctccgaaac
      421 atgttgagaa tgatggttca ggttctgtca caccacgctc tgtaccagct aatttagaag
      481 tgaaaccact caagaaatga tacattgctt ggtctttatt taactttgaa atcggtggaa
      541 taacaccaaa tgcatccgca gttaagaaaa taattgtatt tggatgtgct gctttagatg
      601 gtactacaat attgtcaatg tgattaattg gataagcggc acgcgtgttt tctgtataac
      661 gattgtcttc aaagtccact gaaccatctt ctgcaactac agtgttctct aaaattgcac
      721 catatttgat tgcgtcaaaa atctgtggtt ctttttcttt ggaaagatta attgcttttg
      781 catagcagcc accttcgata ttaaagaccc cgtttttatt ccagccgtgt tcatcatcac
      841 cgattagttt acggtgtggg tcagccgata aggttgtttt accagtgcca gatagaccaa
      901 agaataatgc aacatcgcct ttttcaccaa cgtttgctga gcaatgcatg ctcataatat
      961 cttgcatcgg taagagataa ttcattacag agaagatacc ttttttcatt tcaccagcgt
     1021 attcagtacc gccgattaaa atgactttgt gtttaaatga aataatgaca aaggtttcag
     1081 atttagtacc atcaacttct ggatctgctt taaaatgtgg tgcagaaacg atagtgaagt
     1141 taggtttaat ctttgtagct tcttcttttg attcaggtct aataaacata tttttagcaa
     1201 ataaattatg ccatgctaat tcattgatga ctgtaagttt taacattgta tctttatcgc
     1261 taccagcgta gcctttaaat acgtatagtt catctttttt atctaaatag tctagtactt
     1321 tatggtataa cttcaagaaa gtttcttcat cgataggttg attaatttct ccccaatcaa
     1381 tgttatctct atatgaaggt tcagagacaa aaaatttgtc tttaggcgaa cgaccagtat
     1441 attttccagt acttgcatta acagcaccaa gttctgttaa taccccttcg ttattgtcta
     1501 agattttatt ataaagttgt gtcgtcgaaa gttgaaaatg tgacgttggt tttttcagta
     1561 atttgtcaat tttagttgtt tcagtgtatg tgtctactga catcctaaat ccctccaaag
     1621 cgataattta aatgtaagcc tttacattta attagtataa cacattaaca taattaatct
     1681 acaatatttt accgtaaatt tatttgtgaa aaattgacat taattcatta attaggtact
     1741 attattttta acggattctc ttatcctgag tggtggaggg acatggaccc aatgaaaccc
     1801 agcaacctct tttttataaa agaaaggtgc caaaccgttt gcagacaaat aggtctgaac
     1861 gataagagcg aatggacgta ttaggccttc tctctatatt aatagttaga aggtcttttt
     1921 tatttagctc acagagagag aattttcgta atataaattt aaaggagcaa actatgttaa
     1981 ataacaaacg attatttact tcagagtctg ttacagaagg acacccagat aaaatcgctg
     2041 accaagtgtc agatgcaata ttagatgcta ttttaaaaga cgatccaaat gcacgtgtag
     2101 cttgtgaaac aactgttaca acaggtatgg cattgatcgc cggcgaaatt tctacaacaa
     2161 catatgttga tattccgaaa gttgttagag aaacaattaa agaaattgga tacacaagag
     2221 caaaatatgg ttatgattat gaaacaatgg caattttaac tgcgattgat gaacaatcac
     2281 ctgacattgc acaaggcgtg gataaagcat tagagtatcg tgataaagat agcgaagaag
     2341 aaattgaagc aacaggtgca ggtgaccaag gtttaatgtt tggttacgca acgaatgaaa
     2401 cagagacgta tatgcctcta gcaatttatt tatcacatca attggcaaaa cgtttatcag
     2461 atgtgcgtaa agatggtacg ttaaattatt tacgaccaga cggtaaagtt caggtcactg
     2521 ttgaatatga tgaaaatgat aacccagtac gtattgatac gattgtcgta tcaacacaac
     2581 atgctgagga tgtgacactt gaacaaattc aagaagacat taaagcacat gtcatttatc
     2641 caactgttcc agaaaacttg attaatgaac aaactaaatt ctacatcaat cctacaggac
     2701 gctttgtaat cggtggacct caaggtgatg caggattaac aggacgtaaa atcattgttg
     2761 atacgtacgg tggctatgca cgtcatggtg gcggatgctt cagtggtaag gatcctacaa
     2821 aagtagaccg ttcagctgca tacgctgcac gttatgtggc taaaaatatt gttgcagcag
     2881 gtttagcaga tcaatgtgaa gtacaattgg catatgcgat tggtgttgca gaaccagtgt
     2941 caattgcaat tgatacattt ggaacaggta aagtttctga agggcaactt gtcgaagcag
     3001 ttagaaaaca ctttgaccta agacctgcag gtattattaa aatgcttgat ttgaaacaac
     3061 cgatttacaa acaaactgct gcttatggtc attttggacg tacagatgtc ttattcccat
     3121 gggaaaaatt agacaaagtg gaagaattaa aagacgcagt aaaataataa gatgaattga
     3181 atatagctaa ataaaagatt ttcggtatat aaatgataat atgtatcgaa aatctttagc
     3241 ttttctatta taattaaagt tattcatata ttaaaaagga gcgttgttgt gatggattta
     3301 tcttcaccga tagtcattgg actcattatt gcagtagtca ttgcattaat cttttttgtc
     3361 ctttttttag ttgccttagg tagtaagaaa aaagttaagc gacaaacaga agaaaagtat
     3421 gaacaacaag aacaaaacat taaaaagtct catgaagaag ctttagagaa agagcgtatt
     3481 caaaataaaa agacaattac aaaacaacaa gaagattata atcacatggt tagtacaaaa
     3541 gatcgtgaga ttgacgcatt aaaattgttc tcgaaaaatc atagtgaata tgtaacggac
     3601 atgagattaa ttggtattcg tgaaagatta gttaaagaaa aacgaattcg accagaagat
     3661 atgcacatta tggcaaatat ctttttacca aaggatggct ttaataatat tgaacgaatt
     3721 agtcatttag tattaacaag aactggtctt tatattattg attctcaatt gttaaaaggt
     3781 catgtttata atggtattag tggcggtcaa tttaaagatt taccaccaat ggaacaagta
     3841 tttgatacat tggatttaga taaatctaga ccgcaaacta ttgtgatgga tcaaaatgat
     3901 gacaaacgtt cattatcatt tgtaaattat tcagatcaaa ttgaggcgat taaacaattg
     3961 gctgaagact tacaaaaaga attaggtgcc aaatatacac caacttcaat tttatacttt
     4021 aatcttaaaa atgaaggcga tgtaacgatt tcaaattata atcaaaatag tgctgtcaaa
     4081 gtgcttgtcg gtgctgaaca attagacgag ttctttaaca aatttgtatt ccatggtcgt
     4141 attcaataca acgtggaaga cttacaacaa atgatggata aaattgaatc attcaattaa
     4201 atgttaggct gaagtgatga ctatatgttg ttacttcggc ttttttagtt tgtcataaat
     4261 taaacagaat tacttttaca atttgattaa tatttgagag atattactag aattttagtg
     4321 gttgattgct ctaagtgttt tttctcatgt attcaactca gatttgttat gataacaggg
     4381 tataatacat caaaaaggtg aggcgatatt tatggaggtt aaaacatttt ataatggaaa
     4441 cacgatgcca caaattggtt tagggacatt tcgtgtggaa aatgatgaaa attgcatgga
     4501 aagtgttaag tatgccattg aacaaggata tcgtagtatt gataccgcaa aagtttatgg
     4561 gaatgaagaa caagtaggtg ctggcattcg tgccggattg gagtcaactg gtattgctag
     4621 agaagactta tttattactt caaaattata ttttgaggat ttcggtcgtg aaaatgtagc
     4681 agctgcttac gaagctagtt tatctagatt aggtttaaaa tacttagatt tgtatctagt
     4741 acattggcca ggtacgaacg aagccgtaat ggttgataca tggaaaggta tggaagattt
     4801 atataaaaat aataatgtta aaaatatagg tgtcagcaat tttgaacctg aacatttgga
     4861 agctttgttg gcacaagtgt caattaaacc tgtaattaat caagttgaat atcatccata
     4921 tttaacccaa cataaattga aattatattt ggcagcacaa cgtatcgtga tggaatcttg
     4981 gtcaccattg atgaatgcac aaattttaaa tgatgagaca attaaagaca ttgctcaaga
     5041 attaggaaag tcacctgccc aagttgtttt aagatggaat gtgcagcatg ctgtggttac
     5101 aatccctaaa tcggtgacac caaacagaat ctctgaaaat ttccaaatat ttgatttcga
     5161 attatcagat gaacaaatga cgcgaattga tggtttaaat caagataaga gaattggacc
     5221 tgatccaaaa aaatttgaag gctagattaa aatcgctcaa ctgatgaaaa ggttagatga
     5281 attgt
//
LOCUS       DAMQYT010000170         5211 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMQYT010000170 DAMQYT010000000
VERSION     DAMQYT010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.173"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..179
                     /locus_tag="PGA33_002213"
     CDS             <1..179
                     /locus_tag="PGA33_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferrichrome ABC transporter substrate-binding
                     protein"
                     /protein_id="HDG6474011.1"
                     /translation="SKPSYGKFDFEKTHTWQNIEAVKKGHVISYKAEDYWFTDPITLE
                     HLRSKLKKEILNKK"
     gene            241..1086
                     /locus_tag="PGA33_002214"
     CDS             241..1086
                     /locus_tag="PGA33_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDG6474012.1"
                     /translation="MKQLVGIPESMLIPLIARAKEYENEKPIIKDALSKKIFDGLDDM
                     YKNVTCDDMSQIGISIRTVIIDCVTKRLIKDNKDLIVVNIGCGLDTRFQRFNKEKISW
                     IDLDVPESIEIRKTFFKESNSYKMISKSMLDYSWIDDVKNYKFFNSKSNILFIIEGVL
                     MYFDESVMTQLLDTIIKKMGDHNLTFAIEFCSKTIANNTKRHQSVSKLSSQPVFKYGY
                     NDLKKLNEILPNTIRVIHEYNYFDYYKNRWGLFGYCRFIPYLKKRLNNKIVLMKYKVP
                     KRQRH"
     gene            complement(1458..2681)
                     /locus_tag="PGA33_002215"
     CDS             complement(1458..2681)
                     /locus_tag="PGA33_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDG6474013.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLTDLFDKYDIKS
                     EILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWSYPPFQLTEKDGKLYGRG
                     TTDMKGGLMALVVSLIELKEQNELPHGTIRLLATAGEEKEQEGAKLLADKGYLDDVDS
                     LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNLFKEKYSE
                     LKQQDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIQYLKEK"
     gene            3116..4171
                     /gene="lukH"
                     /locus_tag="PGA33_002216"
     CDS             3116..4171
                     /gene="lukH"
                     /locus_tag="PGA33_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HDG6474014.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKDKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKT
                     VKVVDKYSDDNKPYKEG"
     gene            4193..5209
                     /gene="lukG"
                     /locus_tag="PGA33_002217"
     CDS             4193..5209
                     /gene="lukG"
                     /locus_tag="PGA33_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HDG6474015.1"
                     /translation="MIKQLCKNITICTLALSTTFTVLPATSFAKINSEIKQVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKDKMPVTVSEGFNPEFLAVMSHDKKDKGKSQF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVKFVKVLND
                     NEKK"
BASE COUNT         1987 a          720 c          816 g         1688 t
ORIGIN      
        1 taagtaaacc ttcgtacgga aaatttgatt ttgaaaaaac acatacatgg cagaatattg
       61 aagctgtaaa aaaaggacat gtaatttcat ataaagcaga agattattgg ttcacagatc
      121 ctattacatt agaacatttg agaagtaaat taaaaaaaga aattttaaat aaaaaataat
      181 agaaataagt tgtaaaaatt ttcttatgca ttggtactaa tgtttttaag gagtgattaa
      241 atgaagcaac tggttggaat tcccgaatca atgttaattc ctttgatagc tcgagcaaaa
      301 gagtacgaaa acgaaaaacc aataataaaa gacgcactat caaaaaaaat atttgatggt
      361 ttagatgata tgtacaaaaa tgttacatgt gatgacatgt ctcaaattgg aattagtata
      421 cgtactgtga taatagattg tgttactaag aggcttatca aggataataa agatttaatc
      481 gtggtcaata taggttgtgg cttagataca aggtttcaaa gatttaataa agaaaaaata
      541 tcatggatag atttagatgt accagaatca atagaaatac gaaaaacatt ttttaaagaa
      601 agtaatagtt ataagatgat atctaaatct atgctagatt acagttggat tgatgatgtc
      661 aaaaattata aattttttaa tagtaagtca aatatattgt ttattattga aggtgtattg
      721 atgtattttg atgagagtgt aatgactcaa ttattggaca ctattatcaa aaagatggga
      781 gatcataatt tgacatttgc gattgaattt tgctcaaaaa caattgcgaa taatacaaag
      841 agacatcaat cggtatcaaa gttatcctca caacctgttt ttaaatatgg ttacaatgat
      901 ttaaaaaaat tgaatgaaat tttaccaaat acaataagag taatacatga atataattat
      961 tttgactatt ataagaatcg atggggctta tttggatatt gtagatttat accttattta
     1021 aaaaaaaggt taaacaataa aatagtactt atgaaatata aagttccaaa gagacagaga
     1081 cattaagttc ttagacaatg taaaaaagct gatttctata aattatttga tagaaatcag
     1141 cttttttcaa atgtatttga taatttacag ctcgttgagc tgctgttttc tagggattta
     1201 tgtcccattc cttttacgag aaggttttta cgagttttat gtaactatta gaagctatct
     1261 ttttatatga ccattagtca atatatgtat gacaaatcag cacaataatt tcagttttac
     1321 atcacttaat caatgaaaat gtgcttgttg atatttgaat atttacattc aaacaatatt
     1381 catcgctatt tctatcaact taaaattcgc aataaaaaac tgccagcacg cgaatagggc
     1441 agctgacagc atcgttctta tttttctttt aaatattgaa tggaagcctc tttaaaaata
     1501 tcaatatatt tcagatacat atctttttca atatattcat cgatttgatg tgccattaat
     1561 ggattacctg gtccaaaaat ggctaaatca acattgtcct tattatctcc taagaaacta
     1621 gaggcatctg ttgcacctac aagcgctgaa acaaatattt cgtcttgttc tacataacta
     1681 gaagctacat ctttaatcgt agtaattaat ttgctatttt tatcgcttgt tacaggtcgg
     1741 tgattgcttg gaatatcgag tgaaagctta ttgctatcca catcattaat gatattttgg
     1801 aaaaacgatt ctataaagtc gttatcatac tcaggaactg gtcttacgtt aaattcaagt
     1861 gaagcttcat ctggtacaga gttaaattgt ttgccgccat ttataatcga acatacagct
     1921 gtaagaccag atgcataatt tgcatcctct tcagaaattt cttttccaat caatgatttg
     1981 aacataggcg caacatctaa ttcatgttta gtatcttgtt gtttaagctc tgaatatttt
     2041 tctttaaata gattataaaa ttcaagcagt gtatcaattg cattgtcacc aataaatgga
     2101 actgagctat ggacagcttt accagttgca gttactttac atgacataga ccccttatgt
     2161 gcataataaa ttccagatcc agttggttca gcaataatta agctatcgac atcgtctaaa
     2221 tagcctttat cagctaataa tttggcacct tcttgttctt tctcttcgcc agcagtagcc
     2281 agtaatctaa tcgttccatg aggcaattca ttttgttctt ttaattcgat tagagatacg
     2341 accaaagcca ttaaaccgcc tttcatatct gtagtgcctc ggccatataa tttgccatct
     2401 ttttctgtca gttgaaaagg gggatatgac caattatctt gatttcctgc atcaacaaca
     2461 tccatatgac cactcaatgc gagtataggt gagccgttac cgatttctgc aacgatattg
     2521 gcgcggtgtt cattaacttt caaaatttca gatttaatat cgtacttgtc gaataaatct
     2581 gttaaataat tacaaacgtc tatttcatta ttattttcag tttgtagttc aacaatatct
     2641 gctagtaatt gaattttttc tttttcacta aaagttgtca ttaagctcac acctttcaaa
     2701 gtagtatata tatttatata aacataattt ttattcttaa acataattta aaatgcagaa
     2761 agtagtgtgt tatttgattt cgttctatga aattaactgt ctagtatcaa cgatcttatt
     2821 aacgctgata tcggatatgc ttaattttat agtaaattgt atgagaaaga tgcaggatat
     2881 tatttagcta ataattatat ataaatttca aatcgatggt tattaactac taaataaaaa
     2941 tatttgctaa attaatgact ttgtacacac attattttta aagaggataa agtatttaat
     3001 aatattaaca aaatcattta ataaatagtt aaatatatat tctctgtttt tgtgatatta
     3061 ttcacatgtc gatacatatc aacaaatatc aatcatacga aagaaggtta taacaatgaa
     3121 aaataaaaaa cgtgttttaa tagcgtcatc attatcatgt gcaattttat tgttatcagc
     3181 agcaacgact caagcaaatt cagctcataa agactctcaa gaccaaaata agaaagaaca
     3241 tgttgataag tctcaacaaa aagacaaacg taatgttact aataaagata aaaattcaac
     3301 agcaccggat gatattggga aaaacggtaa aatcacaaaa cgaactgaaa cagtatatga
     3361 tgagaaaaca aatatactcc aaaatttaca attcgacttt atcgatgatc caacttatga
     3421 caagaatgta ttacttgtta aaaaacaagg ctcaattcat tcaaatttaa agtttgaatc
     3481 tcataaagaa gaaaaaaatt caaattggtt aaagtatcca agtgagtacc atgtagattt
     3541 tcaagtaaaa agaaatcgta aaactgaaat attagaccaa ttgccgaaaa ataaaatttc
     3601 aactgcaaaa gtagacagta cattttcata tagctcaggt ggtaaattcg attcaacaaa
     3661 aggtattgga cgaacttcat caaatagcta ctccaaaacg attagttata atcagcaaaa
     3721 ttatgacaca attgccagcg gtaaaaataa taactggcat gtacactggt cagttattgc
     3781 gaatgacttg aagtatggtg gagaagtgaa aaatagaaat gatgaattat tattctatag
     3841 aaatacgaga attgctactg tagaaaaccc tgaactaagc tttgcttcaa aatatagata
     3901 cccagcatta gtaagaagtg gctttaatcc agaattttta acttatttat ctaatgaaaa
     3961 gtcaaatgag aaaacgcaat ttgaagtaac atacacacga aatcaagata ttttgaaaaa
     4021 cagacctgga atacattatg cacctccaat tttagaaaaa aataaagatg gtcaaagatt
     4081 aattgtcact tatgaagttg attggaaaaa taaaacagtt aaagtcgttg ataaatattc
     4141 tgatgacaat aaaccttata aagaaggata atattgaaag ggcggattac taatgattaa
     4201 acaactatgt aaaaatatca caatttgtac gttagcacta tcgactactt tcactgtatt
     4261 accagctact tcatttgcaa agattaattc tgaaatcaaa caagtttctg agaagaatct
     4321 tgatggtgat actaaaatgt atacacgtac agctacaaca agtgatagtc aaaaaaatat
     4381 tactcaaagc ttacaattta atttcttaac tgaacctaat tatgataaag aaacagtatt
     4441 tattaaagca aaaggtacaa ttggtagtgg tttgagaatt ttagacccaa atggttattg
     4501 gaatagtaca ttaagatggc ctggatctta ttcagtttca attcaaaatg ttgatgacaa
     4561 caacaataca aatgtgactg actttgcacc aaaaaatcag gatgaatcaa gagaagttaa
     4621 atatacgtat ggttataaaa caggtggaga tttttcgatt aatcgtggag gcttaactgg
     4681 aaatattaca aaagagagta attattcaga gacgattagt tatcaacaac catcatatcg
     4741 tacattactt gatcaatcta cgtcacataa aggtgtaggt tggaaagtag aagcacattt
     4801 gataaataat atgggacatg accatacgag acaattaact aatgatagtg ataatagaac
     4861 taaaagtgaa attttttctt taacacgaaa tggaaattta tgggcgaaag ataatttcac
     4921 acctaaagac aaaatgcctg taactgtgtc tgaagggttt aatccagaat ttttagctgt
     4981 tatgtcacat gataaaaaag acaaaggtaa atcacaattt gttgttcatt ataaaagatc
     5041 aatggatgag tttaaaatag attggaatcg ccatggtttc tggggctatt ggtctggtga
     5101 aaaccatgta gataaaaaag aagaaaaatt atcagcatta tatgaagttg attggaagac
     5161 acataatgtg aagtttgtaa aagtacttaa tgataatgaa aagaaataag t
//
LOCUS       DAMQYT010000171         5123 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMQYT010000171 DAMQYT010000000
VERSION     DAMQYT010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.174"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            16..1158
                     /gene="scpA"
                     /locus_tag="PGA33_002218"
     CDS             16..1158
                     /gene="scpA"
                     /locus_tag="PGA33_002218"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HDG6474016.1"
                     /translation="MALSLIFSLSVTPIANAESNSNIKAKDKKHVQVNVEDKSVPTDV
                     RNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVLNTEGNIDY
                     IVTISPKITKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVVTQNHKAKL
                     VLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQYINKLENF
                     KIRETQGNNGWCAGYTMSELLNATYNTNKYHAEAVMRFLHPNLQGQRFQFTGLTPREM
                     IYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGHAMAVVGNA
                     KLDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY"
     gene            1189..1515
                     /locus_tag="PGA33_002219"
     CDS             1189..1515
                     /locus_tag="PGA33_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HDG6474017.1"
                     /translation="MEQIELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEALGTSPRMT
                     FIKHKS"
     gene            complement(1807..1980)
                     /locus_tag="PGA33_002220"
     CDS             complement(1807..1980)
                     /locus_tag="PGA33_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HDG6474018.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIVFVGKKIQ"
     gene            complement(1961..2563)
                     /locus_tag="PGA33_002221"
     CDS             complement(1961..2563)
                     /locus_tag="PGA33_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDG6474019.1"
                     /translation="MSFVTENPWLMVLTIFIINICYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            complement(2834..3655)
                     /gene="nadE"
                     /locus_tag="PGA33_002222"
     CDS             complement(2834..3655)
                     /gene="nadE"
                     /locus_tag="PGA33_002222"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDG6474020.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            complement(3648..5117)
                     /locus_tag="PGA33_002223"
     CDS             complement(3648..5117)
                     /locus_tag="PGA33_002223"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDG6474021.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLALGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
BASE COUNT         1723 a          922 c          707 g         1771 t
ORIGIN      
        1 aactttccaa aattaatagc tttaagttta atattttctt taagtgttac ccctattgca
       61 aacgctgaga gcaattcaaa tatcaaagca aaagataaga agcatgtgca agttaatgtc
      121 gaggacaaga gtgttcctac tgacgtacgt aatttagcgc aaaaagacta tctttcttat
      181 gttacttcac ttgataaaat ctataataaa gagaaagcct cgtatacact tggtgaacct
      241 tttaaaattt ataaattcaa caaaaagagt gatggtaatt attattttcc agttttgaac
      301 actgaaggta atatcgatta tatagttaca atttcaccaa aaatcactaa gtattctagt
      361 tcatcttcta aatacacaat caatgtttct ccatttttat ctaaagtctt aaatcaatat
      421 aaagatcaac aaattactat tttgacaaac tcaaaaggtt actatgtggt tactcaaaat
      481 cataaagcta aactagtact aaaaacacca cgtctagaag ataaaaagtt aaaaaagact
      541 gagagtatac ctactggcaa taatgtaacg caactaaagc aaaaagcttc tgtcactatg
      601 ccaactagtc aatttaaaag taataattat acttacaacg agcaatatat aaataaactt
      661 gagaatttca agataagaga aactcaaggt aacaatggtt ggtgtgcagg ctatacgatg
      721 tctgaattac taaatgcgac ttataatact aacaaatatc atgcagaagc tgtgatgaga
      781 tttttacacc caaacttaca aggacaacga ttccaattta ctggtttaac gccaagagag
      841 atgatttatt ttggacaaac tcagggaaga agtccacaac ttcttaatag aatgactaca
      901 tataatgaag ttgacaatct tactaaaaat aataaaggaa ttgctgtttt aggtagtaga
      961 gtagaatcaa gaaatggtat gcacgcaggg catgccatgg cagttgttgg gaacgctaaa
     1021 ttagataatg gacaagaggt tataattata tggaatccat gggataatgg ttttatgact
     1081 caagatgcta aaaacaatgt tattccagta tccaatggcg accattatcg atggtattca
     1141 tctatttatg gttattaaaa tttgtatttt agaaaggtaa ataatattat ggaacaaatt
     1201 gaattattta gtattgataa attcaaatgt aattcagagg ctaagtatta tcttaatatt
     1261 attgagggag aatggcatcc tcaagattta aatgatagtc ctttaaaatt tattctcagt
     1321 acctcagacg attctgatta catttgcaaa tatataaata cagaacacaa acaactcaca
     1381 ttatataata aaaataatag ctcaattgtt attgaaatat ttataccaaa tgataataaa
     1441 atactactaa caattatgaa cacagaagct ttgggaactt ctcctagaat gactttcatt
     1501 aagcataagt cataagacta tctagatatg attaagtaaa ctattaatct tattaaaggt
     1561 ctctaggaag aaaaaatttc atgcgatgag attgttttag aaaaagcgac gatatatata
     1621 atctaacaat aatttttaaa atattaagaa cccccagcat atgctttata tagcttgctg
     1681 ggggtttctc atacaatatg gatcgagcat cttgcatgta taaacaaata ggctattcaa
     1741 agtaaaatta ctcaaacttt gaatagcctt tcctcttcaa atagtcttta tttaaattgg
     1801 attatgttat tggattttct tacctacaaa aacgatttct tgatgtgata cttcaaccgt
     1861 gccatctttt tgctcttgaa acacgggttt ttcaatacgt tttaccggca tatacccttg
     1921 cattttcatt cgtgacaaac aatcagcaat tgtttcattc tcatttactt tgaatttcat
     1981 gttctactac actttccagt tcttctggtt cataatcttt aagctttcta cgacgaatgc
     2041 ctttagtcca gaatccacca tgtatgtttc gaggttcata cgcaatgata aatgctttcg
     2101 gatctaaatt ttttatcgta tccatcaatt tacgttcata ttttcttggt gttaaaattt
     2161 gcatcaccat acgactacca tctctaccaa acgcagcata gtgcgtaacg ccatatccta
     2221 aatttcgaag ttcattcggt aaatctaact catattctgc tgaagttaca tttacaactg
     2281 tataacctaa tgccagtttt tcttctattt tcataccaac aatgatacct attgaaaaac
     2341 caaatgcgta ggcaataata ttttgaatat ggtctaaatt agacataacc aaacctaaac
     2401 caacgatata aactaatact tctaaaaaac taactgatgc agcaatataa cgataacctt
     2461 tcaacgttaa aattgttcgc atcgttaaaa acgttacata acaaatgtta atgataaata
     2521 tagttagtac cattaaccat ggattttctg ttacgaatga cataaggcgc cccactctct
     2581 ttcaattaca ctttaaatac caaactgctt caacctaacc ttaatgaatg catattaata
     2641 taatatagta cgaaaactct cataaataaa tagcgaattt gtcataaata aaaatttatt
     2701 ctcaaatgaa catacttact ttagttcata cactttcaat ttatacactg aagtagtcta
     2761 gctgaatgtg gtttaatgtt attaattcta actcatattt aatttaagtc acacgttaga
     2821 agaaaaaatt aaattaggat tttggccacg tgtatcttgt atatgcaagt tcacgtttgt
     2881 gtgcatttcg tatataatga ttttcaatta ctttttgttc ttctggcgta actggcttac
     2941 cttctaaata attatcaatc gcctcataag ttacacctaa tgcatcttca tctggaagct
     3001 gtggtttatc atcttctaaa tcagcagttg gcgttttttc atataattcc tttggcgcac
     3061 caagatacgc taataattga cgaccttgtc gtttattcaa accaaatata ggtgcgatat
     3121 ctgcagcacc atcaccgtac ttcgtataaa acccagttat attttcagct gaatgatctg
     3181 ttcctactac aataccttgt cggtttgaag caattgaaaa ttgtactttc atacgttcac
     3241 gcgctttttc atttcctttt tggaaatctg taagaacaat accggcttct tttaatgatt
     3301 gcacactttg atcaactgca ggcttaatat tgactgttac tatttcatct ggttcaatga
     3361 atcgcaaagc ttgctcaact tcatcagcat ctttttgaac tccataaggt aatttaactg
     3421 caataaacgt acaatcaatg ccttcttcac gtaattcgtt aacagacatt tgtactagtt
     3481 ttccaactaa tgtagaatcc tgtcctcctg aaatacctaa cactaaagat tttataaatg
     3541 aatgtgattg tacataattt tttataaatt gctttaattc cataatttct tcagcactat
     3601 cgatacgctt tttcactttc atttcttgta caataacgtc ttgtaattta ctcattatct
     3661 tcttccatct ccttaacgtg ttccgcaact tcaaaaatac gtttatgttt attatcccaa
     3721 catgccttgc ttaaatcgac tggatattct tgtggattca ggaaacgctt attttcatcc
     3781 caaatagatt gtaatcctag tgctaaatat tcacgtgatt catcttctgt tggcatttga
     3841 tatactaatt taccattttc ataaatatta tgatgcaaat caatggcttc gaaagatttt
     3901 ataaatttca ttttataagt atgcactgga tggaataatt ttaaaggttg ttcatcgtat
     3961 ggattttcat tttccaaagt aatataatcg ccttctgcct tacctgtttt cttgtttata
     4021 atgcgatata catttttctt acctggcgtc gtaacctttt cagcgttatt tgataattta
     4081 atacgatcac tatatgaacc atcttcattt tcaatagcta caagtttata tactgcacct
     4141 aatgctggtt gatcgtatcc tgtaatcagc tttgtaccaa cgccccaaga atctactttt
     4201 gcaccttgtg ctttcaaact cgtaatcgtt tcttcatcca aatcattaga cgcgataatt
     4261 ttagtttcag taaatcctgc ttcatcaagc atacgtcttg cctctttaga taaataagcg
     4321 atatctccag aatctaatcg aatacctaca aagttaattt tgtcacctaa ttcttttgca
     4381 acttttattg catttggcac gccagatttt aaagtatgga atgtatctac taggaacaca
     4441 caatttttat gtctttcagc atattttttg aaggcaacat attcgtctcc ataagtttgg
     4501 acaaatgcat gtgcatgtgt accagacaca ggtataccaa ataatttccc cgccctaaca
     4561 ttacttgtag aatcaaagcc cccgatgtaa gcagctctag cgccccacaa tgctgcatca
     4621 atttcttgcg cacgacgtgt accaaactcc attaatttat catttgatgc aatttgacga
     4681 attctgctag cctttgttgt aattaatgta tggaaattta caatgtttaa taaaattgtt
     4741 tctattaatt gcgcttgaat caatggtgct tctacgcgta acaatggttc gttaccaaag
     4801 cataattcgc cttcttgcat cgaacggatg ctgcctgtga attttaaatc ttttaaatat
     4861 gataagaaat catccttgta gccaatagac tttaaatatt ccaaatcaga ttctgaaaat
     4921 ccaaaatgtt ctataaaatc aatgacgcgt tttaaaccat taaaaacagc atagccacta
     4981 ttaaatggca tttttctaaa atacaaatca aatacagcca ttttttcatg aatattatca
     5041 ttccaataac tttcagccat atttatttga tataagtcat tatgtaacat taaactgtcg
     5101 tcttctaatt ggtacacttg tat
//
LOCUS       DAMQYT010000172         5115 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMQYT010000172 DAMQYT010000000
VERSION     DAMQYT010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.175"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            41..493
                     /locus_tag="PGA33_002224"
     CDS             41..493
                     /locus_tag="PGA33_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474022.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
     gene            667..1149
                     /gene="ybaK"
                     /locus_tag="PGA33_002225"
     CDS             667..1149
                     /gene="ybaK"
                     /locus_tag="PGA33_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDG6474023.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            1399..2163
                     /locus_tag="PGA33_002226"
     CDS             1399..2163
                     /locus_tag="PGA33_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDG6474024.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            2160..3080
                     /gene="pfkB"
                     /locus_tag="PGA33_002227"
     CDS             2160..3080
                     /gene="pfkB"
                     /locus_tag="PGA33_002227"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDG6474025.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            3086..5044
                     /locus_tag="PGA33_002228"
     CDS             3086..5044
                     /locus_tag="PGA33_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDG6474026.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKAGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFNPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YAFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
BASE COUNT         1805 a          727 c         1037 g         1546 t
ORIGIN      
        1 taagatgata aaaaatgagt aaggaaatgt gggtaagggg atgacagtaa aaaatttatt
       61 tttaggcttt gttgctgtaa tattaaccgt ttgtttaatt ggtttattaa tattagcaac
      121 aaatgaagat gcgcttgcta aggtacataa aacaattaat acgcttaacg cgataaatgt
      181 atcaactgaa gatacttata aaaagaaaat ggatattctc aatattcata ctgctaaagc
      241 atctgaagtg aatgaaaatg tgaaaaagca aaatcatttt aaacatcgtg tgaatgcaaa
      301 taaatcaaat tcttttaacg aacaagagtg ccaagttatt gctgatcgtt atgcagataa
      361 gcatatcaat gataattatg gtttagaaag aatttctaag acaaatcatg gatataatta
      421 tgtgtattcc aatgataatt caactagtaa gcaacatgta agtatttcaa atcaaggcat
      481 aataacgaaa taatagatgg aacagtgtat tctaattgga tatactgttt ttattttgca
      541 ataatttaat ttaaaaaggt gaattcaact tataaaatga tgtaaatgtt atgtcaaaat
      601 caaccaatcc gtaatgtatt ttaaaatgtt aatatagttc tgaagaagta taaatgaggt
      661 gttgaaatgg ctaaaaataa gaaaacgaac gcgatgcgta tgcttgatcg tgcaaaaatt
      721 aaatacgaag ttcatagctt tgaggtacca gaagaacatt tatctggtca agaagtcgca
      781 gaactcatac aagcaaatgt taaaacagta tttaaaacgc ttgttctaga aaatacaaaa
      841 catgaacatt ttgtatttgt tatcccagta agtgaaactt tagatatgaa aaaggcagct
      901 gctttggttg gagagaagaa attgcagctt atgcctttag ataatttgaa aaatgtaacg
      961 ggatacattc gtggtgggtg ttcgcctgtt ggtatgaaaa cattgtttcc aacagtcgtt
     1021 gacaaatcgt gtgaaaatta tagtcatatc agtgtgagtg gtgggcttcg aacaatgcaa
     1081 atcacaatag ctgttgagga tttgattaca ataactaaag gcaaaattgg agcagttatc
     1141 catgaatgat taataacaac aaaaagtatg gggcaagatt aggagtgtga cagagatgaa
     1201 atttttataa aattcatttc tgccacactc ctttttgatt gaattagcat tttacgatca
     1261 taaacagtca ttataattga gtatttgaac ataaaaatgt aattttatcg tcacaatttg
     1321 agtgtttgtg attgtttttg gtaatttatg attgaaaagt gaaagcgtac tcattataat
     1381 acaaagtgag atggggtgat gatgataatt actgaaaaaa gacacgagtt aatattagaa
     1441 gaactttcgc acaaagattt tttgacttta caagaattaa tagatcgaac tggttgcagt
     1501 gcttcaacaa tacgaagaga tttatctaaa ctacaacaat tagggaaact gcaacgtgtg
     1561 catggtggtg caatgttaaa agaaaatcgt atggttgagg cgaatttaac tgaaaaatta
     1621 gcaacgaatc ttgatgaaaa gaaaatgatt gctaaaatag cagctaatca aatcaacgat
     1681 aatgaatgct tatttatcga tgctggttca tctacattgg agctaattaa atatattcaa
     1741 gcgaaagata tcattgtggt aactaatggt ttaacacatg tagaagcttt acttaaaaaa
     1801 ggtattaaaa caattatgct aggtggtcaa gttaaagaaa atacacttgc tacgattggt
     1861 tctagtgcta tggagatatt aagacgatat tgtttcgata aagcttttat cgggatgaat
     1921 ggattagata ttgaacttgg attaactact cccgatgagc aagaggcatt agttaaacaa
     1981 acagcaatgt cattagccaa tcaatcattt gtacttatag atcattctaa gtttaataaa
     2041 gtatattttg ctcgtgtacc tttgctagaa agtacgacaa tcatcacatc tgaaaaagca
     2101 ttaaatcaag aatcgttaaa agaataccaa caaaagtatc actttatagg agggacttta
     2161 tgatttatac agtgactttc aatccttcaa ttgactatgt catttttacg aatgatttta
     2221 aaattgatgg tttgaacaga gcaacagcaa catataaatt cgctgggggg aaaggtatta
     2281 atgtctcgcg cgtcttaaag acattggatg ttgagtcaac tgccttggga tttgcaggtg
     2341 gatttcctgg gaaattcatt atagatacat taaataacag tgcaattcaa tcgaatttta
     2401 ttgaagttga tgaagataca cgtattaatg tgaaattaaa aacaggacaa gaaacagaaa
     2461 tcaatgcacc gggtcctcat ataacgtcaa cacaatttga acaactgtta caacaaatta
     2521 aaaatacaac aagcgaagat atagttattg ttgctggaag tgtaccaagt agtattccaa
     2581 gcgatgcgta tgcgcaaatt gcacaaatta cagcacagac aggtgctaaa ttagtagtcg
     2641 acgctgaaaa agaattggct gaaagcgttt taccatatca tccactattt attaaaccta
     2701 ataaagatga attagaagtg atgtttaata caacagtgaa ctcagacaca gatgttatta
     2761 aatatggtcg tttgttagtt gataaaggtg cgcaatctgt tattgtctcg cttggcggtg
     2821 atggtgctat ttatattgat aaagaaatca gtattaaagc agttaatcca caagggaaag
     2881 tggttaatac agttggctct ggtgatagta cagttgcagg catggtggct ggaattgctt
     2941 caggtttaac gattgaaaaa gcattccaac aagcagtcgc atgcggtact gccacggcat
     3001 ttgatgagga cttagcaaca cgggacgcta tagaaaaaat aaaatcacaa gttacgatta
     3061 gcgtacttga tggggagtga aaataatgag agtaacagag ttattaacaa aagatacaat
     3121 agcaatggat ttaatggcaa atgacaaaaa tggtgttatt gatgagttag taaatcaatt
     3181 agacaaagca ggtaaattaa gtgatgtcgc gtcatttaag gaagcgattc acaatcgaga
     3241 atcacaaagt acaactggta tcggcgaagg tattgccatt ccacatgcca aagtggccgc
     3301 agttaagtca ccagctattg cgtttggtaa atctaaagca ggcgtagatt atcaaagttt
     3361 ggatatgcaa ccagcacact tattctttat gattgcagcg ccagaaggtg gcgcccaaac
     3421 acatctagat gctttagcta agttgtctgg tattttaatg gatgaaaatg tacgtgagaa
     3481 attattacat gcttcatcac ctgaagaagt actagcgatc atagatgagg ctgatgatga
     3541 agtgacaaaa gaagaagagg cagaagctga agcacaacaa gttgcaactg cagaacaatc
     3601 atctaaacaa tctaatgagc catatgtgtt agcagtaact gcttgtccaa caggtattgc
     3661 acacacatat atggcacgtg atgcattgaa aaagcaagcg gataaaatgg gtattaaaat
     3721 taaagtagaa acgaatggtt caagcggcat taaaaaccat ttaactgaac aagatattga
     3781 aaatgcaaca ggtatcattg ttgctgctga tgttcatgtt gagacggatc gcttcgatgg
     3841 taaaaatgtc gtagaagtac cagtagcaga tggtattaaa cgcccagaag aattaattaa
     3901 taaagcatta gatacaagtc gtaaaccttt tgttgcccgt gatggtcaaa gaaaaggtaa
     3961 ctcaaatgac agtcaagaaa aattaagccc aggtaaagca ttctataaac acttaatgaa
     4021 cggtgtttct aacatgttgc cacttgtaat atctggtggt attttaatgg caattgtatt
     4081 tttatttgga gcaaattcat ttaatccaaa aagctcagag tacaatgcgt ttgcagagca
     4141 gctttggaac attggtagta aaagtgcatt cgcgttaatc attccaattt tatctggatt
     4201 cattgcacgt agtattgcgg ataaacctgg tttcgcttca ggtcttgtag gtggtatgtt
     4261 agcaatttca ggtggttcag gatttattgg tggtattatt gcaggtttct tagcaggtta
     4321 cttaacacaa ggtgttaaag ccatgacacg taagttacca caagcattag agggattaaa
     4381 gccaacatta atttatccac tattaacagt gacggctaca ggcttattga tgatttatgc
     4441 ctttaatcca ccagcatctt ggttaaatca tttgttatta gatggattaa acaatttatc
     4501 aggttctaat attgtattat taggtttagt tattggcgct atgatggcga ttgatatggg
     4561 cggtccattc aacaaagcgg catatgtttt tgcaacaggt gcgttgattg aaggtaatgc
     4621 agcaccaatt acagctgcaa tgattggtgg tatgattcca ccgttagcaa ttgcgacagc
     4681 gatgttaatt tttagacgta aatttacaaa agaacaacgt ggttcaatta tccctaacta
     4741 tgtgatgggt atgtcattta ttacagaagg tgcgattcca tttgcagctg ccgatccatt
     4801 acgtgttatt ccttcaatga tgattggttc aggtataggt ggcgcaattg ctttaggctt
     4861 aggttcacga attactgcgc cacatggtgg tattattgta attgttggta ctgatggtgc
     4921 acacttactt caaactctta ttgcacttct agttggcaca ttagtttcag cattaattta
     4981 cggtttaatc aaaccaaagt taactgaaac agaaatcgaa gcttcaaaat caatggacga
     5041 gtagttttaa tgatgtaaaa tgattgttag caaagagctt catattaagt tgtatgttca
     5101 atgaatatat gttag
//
LOCUS       DAMQYT010000173         5090 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMQYT010000173 DAMQYT010000000
VERSION     DAMQYT010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.176"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            251..2224
                     /gene="metG"
                     /locus_tag="PGA33_002229"
     CDS             251..2224
                     /gene="metG"
                     /locus_tag="PGA33_002229"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDG6474027.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKSGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLNES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATKEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            2255..3028
                     /locus_tag="PGA33_002230"
     CDS             2255..3028
                     /locus_tag="PGA33_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDG6474028.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            3195..3731
                     /gene="rnmV"
                     /locus_tag="PGA33_002231"
     CDS             3195..3731
                     /gene="rnmV"
                     /locus_tag="PGA33_002231"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDG6474029.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHAELIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            3742..4635
                     /gene="rsmA"
                     /locus_tag="PGA33_002232"
     CDS             3742..4635
                     /gene="rsmA"
                     /locus_tag="PGA33_002232"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDG6474030.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            4735..4998
                     /locus_tag="PGA33_002233"
     CDS             4735..4998
                     /locus_tag="PGA33_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDG6474031.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
BASE COUNT         1848 a          682 c         1027 g         1533 t
ORIGIN      
        1 ttataaacgc aagctgtaat tttaaatggt aagttatcat tttgcattga tactgataaa
       61 atgatgttga ctatgataaa aaaatgatga catcgacgtt ttttaatgta aaataaatac
      121 attgaaagta ataaatacct taacattgaa taagatgaaa atgagatgac gagataaatg
      181 ttcgcgtccg ttgaaatgca tagaaatctt agatattatt tgaagtgaga cattacgagg
      241 aggaacagtt atggctaaag aaacatttta tataacaacc ccaatatact atcctagtgg
      301 gaatttacat ataggacatg catattctac agtggctgga gatgttattg caagatataa
      361 gagaatgcaa ggatatgatg ttcgctattt gactggaacg gatgaacacg gtcaaaaaat
      421 tcaagaaaaa gctcaaaaat ctggtaagac agaaattgaa tatttggatg agatgattgc
      481 tggaattaaa caattgtggg ctaagcttga aatttcaaat gatgatttta tcagaacaac
      541 tgaagaacgt cataaacatg tcgttgagca agtgtttgaa cgtttattaa agcaaggtga
      601 tatctattta ggtgaatatg aaggttggta ttctgttccg gatgaaacat actatacaga
      661 gtcacaatta gtagacccac aatacgaaaa cggtaaaatt attggtggca aaagtccaga
      721 ttctggacac gaagttgaac tagttaaaga agaaagttat ttctttaata ttagtaaata
      781 tacagaccgt ttattagagt tctatgacca aaatccagat tttatacaac caccatcaag
      841 aaaaaatgaa atgattaaca acttcattaa accaggactt gctgatttag ctgtttctcg
      901 tacatcattt aactggggtg tccatgttcc gtctaatcca aaacatgttg tttatgtttg
      961 gattgatgcg ttagttaact atatttcagc attaggctat ttatcagatg atgagtcact
     1021 atttaacaaa tactggccag cagatattca tttaatggct aaggaaattg tgcgattcca
     1081 ctcaattatt tggcctattt tattgatggc attagactta ccgttaccta aaaaagtctt
     1141 tgcacatggt tggattttga tgaaagatgg aaaaatgagt aaatctaaag gtaatgtcgt
     1201 agaccctaat attttaattg atcgctatgg tttagatgct acacgttatt atctaatgcg
     1261 tgaattacca tttggttcag atggcgtatt tacacctgaa gcatttgttg agcgtacaaa
     1321 tttcgatcta gcaaatgact taggtaactt agtaaaccgt acgatttcta tggttaataa
     1381 gtactttgat ggcgaattac cagcgtatca aggtccactt catgaattag atgaagaaat
     1441 ggaagctatg gctttagaaa cagtgaaaag ctacactgaa agcatggaaa gtttgcaatt
     1501 ttctgtggca ttatctacgg tatggaagtt tattagtaga acgaataagt atattgacga
     1561 aacaacgcct tgggtattag ctaaggacga tagccaaaaa gatatgttag gcaatgtaat
     1621 ggctcactta gttgaaaata ttcgttatgc agctgtatta ttacgtccat tcttaacaca
     1681 tgcgccgaaa gagatttttg aacaattgaa cattaacaat cctcaattta tggaatttag
     1741 tagtttagag caatatggtg tgcttaatga gtcaattatg gttactgggc aacctaaacc
     1801 tattttccca agattggata gcgaagcgga aattgcatat atcaaagaat caatgcaacc
     1861 gcctgctact aaagaggaaa aagaagagat tcctagcaaa cctcaaattg atattaaaga
     1921 ctttgataaa gttgaaatta aggcagcaac gattattgat gctgaacatg ttaagaagtc
     1981 agataagctt ttaaaaattc aagtagactt agattctgaa caaagacaaa ttgtatcagg
     2041 aattgccaaa ttctatacac cagatgatat tattggtaaa aaagtagcag ttgttactaa
     2101 cctgaaacca gctaaattaa tgggacaaaa atctgaaggt atgatattat ctgctgaaaa
     2161 agatggtgta ttaaccttag taagtttacc aagtgcaatt ccaaatggtg cagtgattaa
     2221 ataactgtat ttttaaaaat taggagagat aattatgtta atcgatacac atgtccattt
     2281 aaatgatgag caatacgatg atgatttgag tgaagtgatt acacgtgcta gagaagcagg
     2341 tgttgatcgt atgtttgtag ttggttttaa caaatcgaca attgaacgcg cgatgaaatt
     2401 aatcgatgag tatgattttt tatatggcat tatcggttgg catccagttg acgcaattga
     2461 ttttacagaa gaacacttgg aatggattga atctttagct cagcatccaa aagtgattgg
     2521 tattggtgaa atgggattag attatcactg ggataaatct cctgcagatg ttcaaaagga
     2581 agtttttaga aagcaaattg ctttagctaa gcgtttgaag ttaccaatta tcattcataa
     2641 ccgtgaagca actcaagact gtatcgatat cttattggag gagcatgctg aagaggtagg
     2701 cgggattatg catagcttta gtggttctcc agaaattgca gatattgtaa ctaataagct
     2761 gaatttttat atttcattag gtggacctgt gacatttaaa aatgctaaac agcctaaaga
     2821 agttgctaag catgtgtcaa tggagcgttt gctagttgaa accgatgcac cgtatctttc
     2881 gccacatccg tatagaggga agcgaaatga accggcgaga gtaactttag tagctgaaca
     2941 aattgctgaa ttaaaaggct tatcttatga agaagtgtgc gaacaaacaa ctaaaaatgc
     3001 agagaaattg tttaatttaa attcataaag ttaaaagtga gaaagatcac cgccataaat
     3061 gtaaacgatg ctatattcgt ttaatatgct atggttcttt ctcacttttt taaattaaaa
     3121 tatcgtgcat gtggaatacg tgcgatagag atggttagag ctttgaaatt aagaattgta
     3181 ggaaggcgtt ttaaatgaaa atcaatgagt ttatagttgt agaaggacga gatgatactg
     3241 agcgtgttaa acgagctgtt gaatgtgata cgattgaaac gaatggtagt gccatcaacg
     3301 aacaaacatt agaagtaatt agaaatgctc aacaaagtcg aggcgttatt gtattaacag
     3361 atccagattt cccaggagat aaaattagaa gtacaattac tgaacatgtc aaaggtgtta
     3421 aacatgcgta tattgataga gaaaaagcta aaaataaaaa agggaaaatt ggtgttgaac
     3481 atgccgaatt aattgatatt aaagaagcgt taatgcatgt tagttcaccc tttgatgaag
     3541 cttatgaatc aattgataaa tctgtgctaa tagagttggg gttaattgtt gggaaagatg
     3601 caaggcgccg tagagaaatt ttaagtagaa aattgcgaat cggccattcc aatggtaagc
     3661 agttattgaa aaagttaaat gcatttggtt ataccgaagc ggatgtaagg caagctttag
     3721 aagatgaatg aggaagtgaa aatgttggat aataaagata ttgcaacacc atcaagaacg
     3781 cgagcgttgt tagataaata tggctttaat tttaaaaaaa gtttaggaca gaactttttg
     3841 atagatgtga atatcattaa taatatcatt gatgcaagtg atattgatgc acaaactggg
     3901 gtgattgaaa ttggtccagg catggggtca ttgacagaac aattggccag acatgctaaa
     3961 agagtattgg catttgaaat tgatcaacgt ttaatacctg tattaaatga tacactatca
     4021 ccttatgata atgtgacggt gattaatgaa gatattttaa aagcgaatat taaagaagct
     4081 gttgaaaatc atttacaaga ttgtgaaaaa ataatggttg ttgcaaacct gccgtactat
     4141 attacgacgc caattttatt aaatttgatg caacaagata taccaattga tggctacgtg
     4201 gtgatgatgc aaaaagaagt gggcgaacgc ttaaatgctg aagtaggttc aaaagcatat
     4261 ggttcgttat caattgtcgt acaatactat acagagacta gtaaagtatt aacggtacct
     4321 aaatctgtat ttatgccacc acctaatgtt gattcaatag ttgtaaaact gatgcagaga
     4381 actgaaccgt tagtaacagt agataacgag gaagcattct ttaagttagc aaaagcagca
     4441 tttgcacaaa gaagaaagac aattaacaat aactatcaaa attattttaa agatggtaaa
     4501 caacacaaag aagtgatttt acaatggttg gaacaagcag gtattgatcc aagacgtcgc
     4561 ggtgaaacgc tatctattca agattttgct aaattgtatg aagaaaagaa aaaattccct
     4621 caattagaaa attaaatgat tgacaaagca aagcactatt gttaaaattt aaattttgtt
     4681 tgacgaaaac gttgcaaata tggtattatg taacttgtag cgaggtggag caatatgcca
     4741 aaatcaattt tggacatcaa aaattctatt gattgtcatg taggaaatcg tattgtactg
     4801 aaagccaatg gaggccgtaa gaaaacaata aaacgttctg gaattttaaa agaaacatat
     4861 ccgtcagttt tcattgttga gttagatcaa gacaaacaca actttgagag agtatcttat
     4921 acatacactg atgtgttaac tgaaaatgtt caagtttcat ttgaagagga taatcatcac
     4981 gaatcaattg cacactaaat aagacatata gagatgttag acgtttctta gtataagaag
     5041 taaatattat gataattatt tgagtgttgg gcattatgtt caatactctt
//
LOCUS       DAMQYT010000174         5087 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMQYT010000174 DAMQYT010000000
VERSION     DAMQYT010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.177"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(28..726)
                     /locus_tag="PGA33_002234"
     CDS             complement(28..726)
                     /locus_tag="PGA33_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HDG6474032.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQQETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(743..1732)
                     /gene="floA"
                     /locus_tag="PGA33_002235"
     CDS             complement(743..1732)
                     /gene="floA"
                     /locus_tag="PGA33_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HDG6474033.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(1750..2433)
                     /locus_tag="PGA33_002236"
     CDS             complement(1750..2433)
                     /locus_tag="PGA33_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDG6474034.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
     gene            complement(2677..2853)
                     /locus_tag="PGA33_002237"
     CDS             complement(2677..2853)
                     /locus_tag="PGA33_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDG6474035.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
     gene            complement(3146..4492)
                     /gene="mtaB"
                     /locus_tag="PGA33_002238"
     CDS             complement(3146..4492)
                     /gene="mtaB"
                     /locus_tag="PGA33_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDG6474036.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            complement(4499..>5087)
                     /locus_tag="PGA33_002239"
     CDS             complement(4499..>5087)
                     /locus_tag="PGA33_002239"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDG6474037.1"
                     /translation="ITSDKIEVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKAT
                     ELGASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKL
                     ICDTIDNYDYILIAYEEEAKDGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLF
                     SNTSTVVGLGPRILRAETAPLYALSAISYEKELMG"
BASE COUNT         1547 a          976 c          722 g         1842 t
ORIGIN      
        1 tacgatagtg cttgttttca aaaaatttta taattgtttt ggtttagcta aaatttctga
       61 ccatatcata ccattgatta cttcatcttt atcaaattgg aacgctgatt tagtcaaatc
      121 tttttcgaca ttttgagaat taagcagctg ctttaatttc aaacgttttg tacgttcaga
      181 taagtattta tcctcaataa tatctctagc acgtttttcc attttagcaa tttgcttttc
      241 tttttctcta tcaatgtcag tacgaatcgt tttaatatca tctctaagtg atttttctaa
      301 ttgacgtcta atttcgtcag aattatcttg tctagaaggt ctatgtttct gctccaccgg
      361 ttctgcttta ggcactgtga ttggtttctc tgtcatcggt tttgtttctt gttgtttttt
      421 aggtgccata ggttctacaa caggtttcga tttaggctct tcctttggaa gttcatcgaa
      481 taaaggtggt aacgtatcat catattttcg tttcgatgat ttcttttctt cttcatttaa
      541 ttcttcactt atttctttaa acgttcgctc aatttcttca aaaaagccac ctttttttgg
      601 ttcattatcg gtagatgttt tttgaggtgg cttttgattt tgtctatctt tatgactatt
      661 ttcgcgcata gtagtaatga tagaaatgat cactgatatg acaaaaatta gaataccgac
      721 actcatttaa tcacctctcg acttaatgtt caggtgactc atcatcactt tgatcagttc
      781 gtttattaat tgcatttctc atgcctgtat cagcttcgat atttttcaaa ttataataat
      841 ctttaacact gatattacct gaacgtaatg cttcagccat agctaatggt acttcagatt
      901 cggcttcaac tactttagca tgcatttctt gtacacgcgc tttcatttct tgctcagttg
      961 ctacagccat agctctacgt tcttcagctt ttgcttgtgc aatatttttg tctgctaatg
     1021 cttgttcagt ttgtaagtct gcaccaatat ttttactaat atcaacgtca gcaatatcaa
     1081 ttgataaaat ttcaaatgca gtacctgaat ctaaaccttt gcttaaaact gttttagaaa
     1141 tattatctgg gttttcaagt acttctgtat gatgcttact agaaccaatt gttgaaacga
     1201 taccttcacc aacacgtgcg atgattgttt cttcaccagc accaccaaca agtcgagcaa
     1261 tattagctct aactgtgata cgagctttgg ctttcacttc aataccgttc attgctacac
     1321 ctgcgataaa tggtgtttca atgactttag gattaacaga catttgaacc gcttctaata
     1381 cgtcacgtcc tgcaaggtca attgcagcag cacgttcgaa aggaagatca atgtcagcac
     1441 gttgtgcagc aatattagcg tcaacaactc tgtcaacatt tcctcctgct agataatgcg
     1501 attctaattg gtttgttgtt aatgctagtc ctgctttgtg cgctttaatt aatggcgcta
     1561 taacttttct tggagataca cgacgtaaac gcataccaac caatgtacct ataccaacat
     1621 gaacgccagc tgctaacgct gaaatccata aaccaatggg tacaaatgag aataaaataa
     1681 gtaatgcaac tactataata actgctatta cgataaaact taaactaaac atggtatcgc
     1741 tccttttaat taatctactt ccctcacaac tactcttgtt ccttcaactt caaggatttt
     1801 taccgtttta ttgcgcaaaa taaagttgcc atctgaaaca gcatcaatac gttcattttc
     1861 acaaaaaata atccctgcag gtcgaagatc tgtaactgtt tgagcagtct ttcctacgag
     1921 gtgcgagcgg ttatcatgag aattgtaacc tgactcagaa ttagttgaat cttttaagat
     1981 aactttatcc aaaaacggaa tctttctgtt gaaaatcttc actaatatca cccattctac
     2041 aatcgttaaa atcaaggcaa cgataacatt cgcaagcata aatagcaaat tatcaccgag
     2101 cgttgttatg cttatagtta tcagtatcat gccaataata ccaattactg caccaactac
     2161 aaataattca attacaacta atataacgcc aattgagaat attaagatag aatgcatatt
     2221 gacatttcct tggattagaa atcccaaaaa taaaataagt aatgataatg tggcgataat
     2281 accagctgca ttgatttttt tagagtaaag ttgatataca aatcctaaga atgtcaaaca
     2341 ggttaatatt aacgtaaaaa taggttgaac aattatattg ctaacttgtt caacccaagt
     2401 atctccagcc gttgattcca agatagttgt catttgtaaa aaattattat aactcacaat
     2461 ttcacctcgc cctcacatta attatacatg aaattgaata tactttatag ttataagaac
     2521 ctatagaata tcaatttgat tatttataaa ctatataact ttttttaatt aaaaattgac
     2581 aacaaaaaaa catcacatat tagtgtcaga cactaacttg tgatgtttgt tttatataat
     2641 taatattcgt gttgagggag tcaacagagg tattaattat ttgaatttac gtttacgtgc
     2701 agcttctgat ttctttttac gttttacgct tggtttttcg taaaattcac gtttacgtac
     2761 ttcttggatt gttccacttt tagaaactga acgtttaaat ctacgtaacg catcttcaag
     2821 tgattcattt ttacgtacta ctgttttaga catctgtatt tccctccctc caaatatcaa
     2881 cggaacttta tagtcaattg cacagtaaaa ctatgcattt ataagtataa tatatctgta
     2941 atttatggtc aattagtaaa ttgtttttta tttgaaacat atttcacatc aaaatcacaa
     3001 agacttttag attttgttct aaaaatctct taataattta tttaatgaga agagttgctt
     3061 atatagtaaa ttgtgaagcc gttaaaacaa cgttacaaaa cctatatctt taatacggaa
     3121 ccatatggta tgaatcaagg aatacttaaa ctaaaacttc tctatcagat ttatttgttg
     3181 cgaaatcaac aactttaatt gcttgccctt catttaatgg ataatttgct tgcgtaattt
     3241 taacttttac aatttgacct atgagtgatt cgtcaccttc aaattgtact ttcatataat
     3301 tatctgcata tccaactaat gtaccttctg tgtcaccctg ttcctcagga attacttcaa
     3361 gcacatcttg atcaaattta gacgcatata actttccgag ttgattgctt agcgtaatta
     3421 acttatgcac ccgttcattt ttaatttctt catcaatttg gtcatccatt cttgcagctg
     3481 gcgtgccaat tctaggagaa taagggaaaa catgcagttc agagaactta tgctttacga
     3541 taaaatcata tgtttcttgg aactcagctt cagtttcacc tgggaaacca acaattacat
     3601 cactcgtaac tgccaagtct ggtaaagctt tatgcaattt tgttaatcgt tctgaaaatc
     3661 tatccattgt atacttacgt ctcatacgtt ttaatactgt atctgaacca gattgtaatg
     3721 gaatatgcaa atgacgcaca acttttgttg aacgttctaa aacgtcaatt acttcatctg
     3781 taagttgact tgcttcaatt gaagaaattc gaattcgttc taatccatta atcgtttcaa
     3841 gatcacgtaa taattgggcc aagttataat cttttaaatc ttgaccatat ccacctgtat
     3901 gaattcccgt caatacaatt tccttatatc ctgaattcac tagttgcgtc gcttgttcaa
     3961 ctactttttc cggatctctt gaacgcatta agccacgagc ccatggaata atacagaatg
     4021 tgcagaagtt gttacaacct tcttgaattt ttaatgacgc acgtgttcta tctgtaaaat
     4081 aagggacatc taattcttca tatttacgat ttttcatgat atttccaaca ccattaattg
     4141 gttggcgttc tttacggaat tcgtcaatgt aacctaatag tttatgtcta tcttgtgtac
     4201 caactactac atcgacacca ggaatttcca taatttcagc tgatgaagtt tgcgcataac
     4261 aacctgttac acagattaca gcatcaggat tttgtcttat tgcacgtcta attatttgac
     4321 gacttttttt atcacccgta ttcgttactg tacaagtatt aataacaaat acatcagcat
     4381 tcgcttcaaa gtcaacgcgc tcatagtttg cttctttaaa taattgccag attgcttcag
     4441 tttcataatg gtttacttta caacctaatg tgtgaaacgc aactgttgac ataaatattc
     4501 accccattaa ttctttttca taacttattg cacttaacgc atacaatggc gcagtttctg
     4561 cccgtaaaat tctcggccca agaccaacaa ctgtactagt attactaaat aatgaaattt
     4621 cattttctga caaaccaccc tcaggaccaa aaatcatcaa cactttatcc tgagcattga
     4681 attgttgtaa agtttgcttg aaattgctta actcaccatc ttttgcttcc tcttcatatg
     4741 caataagaat atagtcataa ttatcaatag tatcacaaat taattttaaa ttcgactcga
     4801 attgaataga tggaatcact aaacgatagc tttgttcagc agcttcttta attatttttt
     4861 gccaacgctc tatctttttg gcaacttttg cctcgtttaa tttaacaatt gaacgttcca
     4921 tgctcacagc tataaatgat gaagcaccca attcagtagc tttttgtagc aaccactcat
     4981 atttgtcagc tttgattagt ccactgcaaa tcgtaacatc aactggcaat tctgtattaa
     5041 tattttgttt ttcttttaaa tcaacttcaa ttttatcact tgttatg
//
LOCUS       DAMQYT010000175         4982 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMQYT010000175 DAMQYT010000000
VERSION     DAMQYT010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.178"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            184..1674
                     /locus_tag="PGA33_002240"
     CDS             184..1674
                     /locus_tag="PGA33_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDG6474038.1"
                     /translation="MHRESKYIEYKKSRKGLSNDIWSTYSAFANTEGGTIYLGIEEKK
                     IEDKKVFVSVGVEDPEKMIEDFWNALYGRSKVSQNILSNKDVKIVNIENKACIEIHVP
                     EAPYSKKPIYVDNKKDLVYKRVDDADRIATEEEYKFMIVNSQDDIDTELLDNYDMSDL
                     NHESIENYRKLLLKNTNDERYANMSQLDLMIDLGAYRKDRSSKDKQYKMTTACLLFFG
                     KYNAISDRFPGFQLDYFKKTNYLDTDWKDRISSGDLGNEDLNVYSFFEKVLIKLTDNI
                     EESFSLNDGLTRQNYARDLKVAIREALVNTLMHAYYDTKQSIKIVNCEDFIEFYNPGN
                     MRINKEDFIHGGHSKDRNSILSTLFRRVGYSEKAGSGGPRIFDVVNRHKLKTPEIELT
                     DMDTNVVLWKQDLMKEFEKYPELDKKVIKYIIDYGSISKGEALKMENMTEYQFRNILK
                     KLKDDNLIKKEGEGPATKYVLIESKEADILRTKKVIKSLESFFRNK"
     gene            complement(2047..3365)
                     /gene="cstB"
                     /locus_tag="PGA33_002241"
                     /pseudo
     CDS             complement(2047..3365)
                     /gene="cstB"
                     /locus_tag="PGA33_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002488497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
     gene            3421..3558
                     /locus_tag="PGA33_002242"
     CDS             3421..3558
                     /locus_tag="PGA33_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001791883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474039.1"
                     /translation="MELKLLNKLDDDQLDLEFLQDNGLKGNVQGPMTLKEPLKSYIQK
                     S"
     gene            3555..3926
                     /locus_tag="PGA33_002243"
     CDS             3555..3926
                     /locus_tag="PGA33_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDG6474040.1"
                     /translation="MKTNILNLDNELENTEYVEGKPYVQYGIKYERNQALRNEAIKIH
                     GTTCKVCGFDFKAKYGDLGEGFIEIHHLKPMFSIKREIKVNPQKDLVPLCSNCHKMIH
                     RNTKKPLTIKELTKIVNYNSK"
     gene            4055..4675
                     /locus_tag="PGA33_002244"
     CDS             4055..4675
                     /locus_tag="PGA33_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474041.1"
                     /translation="MKFTEVEVIEHLVKAYKEAGKPTYPHENLYRGRNHSISGIGEDL
                     LGAYLISRLEGVQIFIDQPLSMIDKSLSTRYPDLLICEDNEIKNILEVKMDLGYQRKD
                     FIDYCRKKEEWISNIVGKQCVLSRKREDKIPMNIADDIKFHVVIYSENNGPKRFDEEI
                     MPIVNETCPHIEVYVLTSGQHPNLVNVNLEGININKDEFEILVNAL"
BASE COUNT         1853 a          610 c          879 g         1640 t
ORIGIN      
        1 cgtgaaataa tcagaggaat cgtttgaaat catcgtagta atcgcgcgaa aatcgcgtga
       61 aataatcaga ggaatcgttt gaaatcatcg tagtaatcgc gcgaaaatcg cgtgaaatac
      121 tatggtagac ggtttgagta aattaatgga gtattttaat atttatggtt tggaggaatt
      181 gcgttgcata gagaaagtaa atatatagag tataagaaat cacgaaaagg attatctaat
      241 gatatttggt ctacgtatag tgcttttgca aatactgaag gtggtactat atatttagga
      301 attgaagaaa aaaagatcga ggacaaaaaa gtctttgttt cagttggtgt tgaagatcca
      361 gagaaaatga ttgaagattt ttggaatgca ctatatggaa gaagtaaagt tagtcaaaat
      421 attttatcaa ataaagatgt taaaattgtt aatattgaaa ataaagcgtg cattgaaatt
      481 catgtaccag aagcgcctta ttcgaagaaa ccgatatatg tagataataa aaaagattta
      541 gtatataaaa gagttgatga tgctgataga attgcgactg aagaagagta taaattcatg
      601 attgtaaatt ctcaagacga tatagataca gaattattag ataactatga catgtctgat
      661 ttaaatcacg aatctatcga aaattatagg aaacttctat taaaaaatac taatgatgag
      721 agatatgcga atatgagcca actggattta atgatagatt taggagcata tagaaaagat
      781 agaagttcga aagacaaaca gtataaaatg actacagcat gtttattatt ctttggtaag
      841 tataatgcga ttagtgatag attcccagga tttcaattag attattttaa gaaaacaaat
      901 tacctagata ctgattggaa agatagaata tcaagtggag atttaggtaa tgaagattta
      961 aacgtgtata gtttttttga aaaagtattg ataaaattaa ctgataacat tgaggaatca
     1021 tttagcctaa atgatggttt gactagacaa aattatgcaa gagatttaaa agtagcaatt
     1081 cgcgaagcac tggttaatac attaatgcat gcgtattatg atactaagca aagtattaaa
     1141 atagttaatt gtgaagattt tatagagttt tataatccgg gtaatatgag aataaataaa
     1201 gaagatttta ttcatggagg gcattcaaag gacagaaata gtatattatc gacgcttttc
     1261 agaagagtag gatattcaga aaaagctgga tctggaggac caaggatatt cgatgtagtt
     1321 aatagacata agcttaaaac gcctgaaata gaattaacgg acatggacac taatgtagta
     1381 ctttggaaac aagatttaat gaaggagttt gaaaaatatc ctgagttaga caaaaaagta
     1441 ataaagtata ttattgacta tggatcaata agtaagggtg aagccttaaa aatggaaaat
     1501 atgacagaat atcagtttag aaatatttta aaaaaactaa aagatgataa cttgataaaa
     1561 aaagaaggtg aaggtccggc tactaaatat gtgttaatag aatcaaaaga agctgatata
     1621 ttgcgaacta aaaaagtaat taaaagttta gagtctttct ttaggaataa ataaaaaaac
     1681 aagagatagg tgcgaagtgt ttggattata caaatgcttc gcatcttata tttattaata
     1741 aatatcatag aaaaccgtat cattaaccga tacgcagaga tgcggttttt tagacacttc
     1801 ataaagggat tttgaacgta tcagaacata tgaggtttat aggaattgct gttatgtttt
     1861 ttgatcacat caataaacaa aaaaggtatg tactatgtaa aatatttatt aaatgatata
     1921 aagcgagggt atataaatga tttttaaata gatatttatc caataatata aaaaggaact
     1981 ataagctata tctaaaagct atatctaact acttatagtc cttcttcatt agtataaata
     2041 taattattaa gaaaggtata tatctttgta accttcgttt acattaataa tgttgtgata
     2101 acctttgtgt tctaaaatac caatagctat agaacttcta atgccagact gacagtctac
     2161 ataaataaca tcgtttctat tgaaaggtaa atctgtttct aaaagtttgc cgtgtggcac
     2221 atgaaccgct tgggataagt ggccattatt ccattcatta tcattacgta catctaagac
     2281 atgtgcttca ttaccagtta tgtctttact atgaacagat tgtgtttgaa tttgagcttg
     2341 tggtaactga tatccagaca cattatcata tccaataagt tgtaaagtat gttatgtgtt
     2401 gcttttgaaa caaggtgata gtctccaatc aagttaattt cttgattata gtttagatac
     2461 cagccaattt gattgatgaa atttttatca tatggaatat tgattgtacc ttcaatatgt
     2521 ccaccatgat aagcctcctt actgcggaga tcaaaagtta atctgtttgt acttgtagct
     2581 ggataaaccg tataaggttg atataaattc ataccgaatt gattaatttt tttcatttgt
     2641 gcaaaatgat gtggtggtgc aggttggtca gaaatgagtt tatcgataaa ggtagcttca
     2701 ttattttcag aaaaagccca gttcgtttgt ttttcatagc caagcgtaga tgttggaata
     2761 gcacctaaag atttaccaca aggactacca gcgccatgac caggccaaat ttgaatgtag
     2821 tctggcaagt ctttaatact ttcaatagat ttaaacattt gttttgcgcc tatttcagat
     2881 aatccttcta ctttaacagc tttttctagt aaatcaggtc taccgatatc tcctacaaaa
     2941 ataaaatcac cactgaatag tcccattgga acttgtgctc cagcaccttc gtcagtaagt
     3001 aaaaaactta tactttctgg cgtgtgacca ggtgtatgaa gcacttttaa ttttatattt
     3061 cctacataaa tatcatcatt atgttgaaca aaatgagtgt ggttaggcat atttttataa
     3121 cctaacatgt catcactttc gcccgataca taaatactag catttaactt tatagcaaca
     3181 tctctaattc ctgaaacaaa atttgcatgt atatgtgttt cagctgcatg agtaatggtt
     3241 aaactctctt catcggcaac tcgaatatat gaagataagt cacaaatagg atcaatgatc
     3301 atggcttctc cagttttttg acaaccgatt aaataagatg cttgagataa atgtttatca
     3361 taaattgatg atagtcaatt tgttatcaag ggtgataatt atattaacta tggtaattac
     3421 atggaattaa agttattaaa caaacttgat gatgaccaat tagatttaga atttttacaa
     3481 gataatgggt taaaaggtaa tgttcaaggt cctatgactt taaaggaacc tttaaaaagt
     3541 tatatacaaa agtcttgaaa acgaatattt taaatttaga taatgagtta gaaaacactg
     3601 aatatgtcga aggtaaacca tatgtacagt acggtattaa atatgaaaga aatcaggcat
     3661 taagaaatga agctattaaa attcatggaa ctacatgtaa agtatgtgga tttgatttta
     3721 aagctaagta tggcgattta ggtgagggtt ttattgaaat tcatcattta aaaccaatgt
     3781 tttcaataaa aagagaaata aaagtaaatc cacaaaaaga tttagtccca ctatgttcta
     3841 attgccataa aatgattcat agaaatacta aaaaaccttt aacgattaaa gaattaacca
     3901 aaatagttaa ttataatagc aaataattta atattttata aactatcatt caaccctctt
     3961 aatttattag gaggtttttt gtatttatgc tttcaaatgt gtgatatact ttgtttgttg
     4021 aaatatagag tatctataga tagggtgatt gagtatgaaa ttcactgaag tggaagttat
     4081 cgaacatctt gtaaaggcat ataaagaagc aggaaagcct acttatcctc atgaaaattt
     4141 atatcgagga cgtaatcata gtatttcagg tattggagaa gacttgctgg gtgcttattt
     4201 gattagtaga ttggaaggtg tccaaatatt tattgatcag cctttatcta tgattgataa
     4261 atctttaagt acaagatatc cggatttatt aatttgtgaa gataatgaaa ttaaaaatat
     4321 actagaagtt aaaatggact taggatatca aagaaaagat tttatagatt attgccgaaa
     4381 gaaagaagaa tggatttcaa atatcgtagg aaaacagtgt gtattgtcta gaaagagaga
     4441 agacaaaatt cctatgaata tagctgatga tattaaattt catgttgtga tttacagtga
     4501 aaacaatgga ccgaagcggt ttgatgaaga aatcatgcct atcgttaatg aaacatgtcc
     4561 acatattgaa gtatatgtcc taacaagcgg tcaacaccct aatttagtaa atgttaatct
     4621 tgaaggtatt aatattaata aagatgaatt tgaaatatta gtaaatgcgt tataaaaaaa
     4681 tagagcatcc tccacgttat ggaggtgctc tgttttttat tgaaaagtat caagttaatt
     4741 aatttaatat gcttaataag ttctaccttg acctttttct ctagcttctg ttcgatctct
     4801 tatgtactca gtacatactg gattttctgt taattcatca actgtattag tttaatcaga
     4861 agacgtgtct actttgtaag cttctaagaa cttattatat gatatagcgt ttgagttttg
     4921 ttgttcttct ataaatttct gtagttattt ttcaaaaacc gcatcattaa ctgataagca
     4981 ga
//
LOCUS       DAMQYT010000176         4981 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMQYT010000176 DAMQYT010000000
VERSION     DAMQYT010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.179"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..603)
                     /locus_tag="PGA33_002245"
     CDS             complement(<1..603)
                     /locus_tag="PGA33_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDG6474042.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLERQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKEMKDFKLNDIQNHTLLVTEKGCSYREQLE"
     gene            716..1266
                     /locus_tag="PGA33_002246"
                     /pseudo
     CDS             716..1266
                     /locus_tag="PGA33_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
     gene            1259..2479
                     /locus_tag="PGA33_002247"
     CDS             1259..2479
                     /locus_tag="PGA33_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDG6474043.1"
                     /translation="MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQL
                     LGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETY
                     LVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGIL
                     YKINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNM
                     MIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAI
                     LSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMN
                     TVSDKYVGRSFTIWTSISLLLQSLIAPFLGRWINEINDKFGFYIILILSLLIFVTLLL
                     VNKTGKIKYAHKEE"
     gene            complement(2551..2847)
                     /locus_tag="PGA33_002248"
     CDS             complement(2551..2847)
                     /locus_tag="PGA33_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDG6474044.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKRRKMMGEYIIYYDGVVIGGLYDN
                     RLLVKATKSAQQKLQDNTLVSPYPGSKEMILILDFTEATNLTDLFKTIKNDLKK"
     gene            complement(2896..3099)
                     /locus_tag="PGA33_002249"
     CDS             complement(2896..3099)
                     /locus_tag="PGA33_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474045.1"
                     /translation="MEGLKHSLKSLGWWDLFFAIPIFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            3408..4394
                     /locus_tag="PGA33_002250"
     CDS             3408..4394
                     /locus_tag="PGA33_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDG6474046.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL"
BASE COUNT         1701 a          770 c          783 g         1727 t
ORIGIN      
        1 ttctaactgt tctctgtaac tacacccttt ttcagtaaca agtaatgtat ggttttgaat
       61 atcgtttaat ttgaaatctt tcatctcttt gttagtaaca aatattaaac gttcttcagt
      121 tataggaata gcagttaagt tactattaat aatcttttcg tctgtgataa ggctaatatc
      181 cgatttgaaa ttagcaattt cttctttaat atagaaatta gaatgtgcgg atttaaactt
      241 caagtttaca tatgggtgtt cagctaacca ttcactaatc ttattatagt agcgatacaa
      301 atactgactt tcttgcatgt taatacttat atttaccttc ttattgtaaa gtccttttat
      361 ttctctctcg tattcgctat tactatctaa aatcacttta gcgtatttaa cgtatgctaa
      421 gccttcttcc gttagttcca taccatattt ttttctataa aataatttaa catctaattg
      481 cctttctaat ttttcaatat gtgtagatac agttgactgt gaatagttaa gtaactctga
      541 tgctttagta aggttctttg tttcactagt tactaaaaat gattttaaaa tgtttaagtt
      601 caaagtagta cctcctttag ggttcccttt tatcgaatcc tgaattctaa gaattctgat
      661 ttaataataa tatattaaat attattataa aggtaaatcg aaaggagtta tgactatgat
      721 tacttgttta tttggaagta gtagaaaggt ggcaattcag ctatcgcagt agagaatcta
      781 ttgaagaatt taaaagtcaa ctttgtgaat ttatatcaaa gcaacattga aaaggttaca
      841 gataatcgtc atagcaaagg tgccaacttt cataatgatg attacgaaca aattttaatc
      901 aaagttttag aatcagatat tattattttt tcaacaccac tctattggta ctccatgagt
      961 gcgtctttaa aactcttcat tgatagatgg accgagtcat tgcgagatac gcaaatagac
     1021 aacttcaaag aaatcatgtc acaaaaaaag tatttgattt taatcatagg tggcgacaat
     1081 ccgcatacca aagcacaacc tttagtcaac caattcaaat taatatttga gtttatgaat
     1141 attactaact atagattttt gatcggcgaa ggcaataaac cattcgatat tttaaacgac
     1201 tctcaattta tagaagagct cgcaaatata aatcttgcat tgaaaaaagg tgatatttat
     1261 gactgatttg ttgaaaatta aaaacttcag attgttcttt ttagcagaaa taatttcagc
     1321 attcggagtt ggaattagta cagttggggc taattggtat ttaatcgaca aaactaacga
     1381 ctcccaacta ttaggtatta tgttagcttt aaatgtatta tccggtttct tagcttctcc
     1441 tataataggt ggactagccg ataaatataa tagacgaaat attattttaa tcacctatct
     1501 tcttcaggta atattatatt tactcattgt catagcactc gtaatgatag gatttgagac
     1561 ctatttagtg attggatttg cgattgttaa tgggataggt tggactacgt atatggcaac
     1621 atctagaagt ttagttaaac agatattaaa accagatcaa tatacagatg caaattctct
     1681 attagagatt agtttgcaaa caggtatgtt tattgcaggt ggattatcag gaatattata
     1741 taaaataaat ggattcactc taattatagc gatgactata atgatgtttc taatcagcat
     1801 ttttatgtta tttagattgc atgtagataa accaactcat tcagaggaag aatcaacaaa
     1861 tagcttattg caagaatatt tattaggatg gaaattttta aaagacaaca tgatgatatt
     1921 tatttttgga gttatttcaa tcataccaat ggtgtttaca atgatcttta acatatcatt
     1981 accaggatat gtttataatg ttttaaaact ttcgtctgta caatttggtt tttcagatat
     2041 gttatatggc attggaggat tatgtgcagg tttaatttca gctattcttt cgaagaaaat
     2101 ctcaactaaa gtattgatat ttttgttata tttcatatta gtcataaatt cagcactatt
     2161 tatttggata aactcagcat tttacttatt cataggatca tttatactag gctactcaat
     2221 ttcatcaatc agaatttata tgaatacagc tataatgaac actgtttcag ataaatatgt
     2281 cggtcgctca tttacgatat ggacgtcaat ttcattgtta ctgcaaagtt taattgctcc
     2341 atttttagga agatggatta atgaaattaa tgataaattc ggtttctata ttatactcat
     2401 tttatcctta ctcatatttg ttacactgct gcttgttaac aaaacaggca aaataaaata
     2461 tgcacataaa gaagagtgac cgtcactctt ctttaacaag cgaccattta tcgatgggct
     2521 tagttctctc tgcacccaca cttcactact tcactttttc aaatcatttt ttatggtctt
     2581 aaataaatca gtgagatttg ttgcttcggt aaagtctaga attaatatca tttctttaga
     2641 acctggatat ggcgaaacta atgtattatc ttgcaatttc tgctgggcac ttttagtcgc
     2701 cttgaccaat aatctattat catacaaacc acctataacc acgccatcat aataaataat
     2761 atattctccc atcatctttc ttctcttaac cgcgtttgaa ttcacatgat ttaaaaataa
     2821 atcatgtaca tctttcttag tcgccattgt aatcgctcct tcagttttat gtttaatcac
     2881 attcgtatta atgattcatt tcgtgttgct cttaatttta tctataatta tatgcgtagt
     2941 taaaatcaaa cctatggaaa agaaaataat gataacaatg ttaagaaata tagttataaa
     3001 attatagttt ggaaggtatg cgaatagcag aaaaataggt atcgcaaaaa ataaatccca
     3061 ccaacctaaa ctttttaaag aatgctttaa accttccata atatcacctt tataaatttg
     3121 tctttgttat aagataacta aaaaatcgct ttactgtaaa agtagccaaa gaaaattctg
     3181 aatcatattc ataagtagtg tatcattaat aatgaacaat ttaatactat aatccttgat
     3241 ctttgtattg atcaacttac cacaacattt attttagact actcttagac ttccctttca
     3301 aatggttgca tctattgaaa ttccttttgt ataagttagg cttttgtggt aatatcatca
     3361 tgcataaaaa atcgagatac taattataaa gagggtataa atatattatg aaagaaaatt
     3421 tttggagtga attaccacgt ccatttttta ttttggcgcc aatggaagac gttacagata
     3481 tcgtctttcg acacgttgta agtgaagcag ctagaccgga tgtgtttttc actgaattta
     3541 caaatactga aagcttttgc caccctgaag gcatacatag tgtgcgcgga cgcttaactt
     3601 ttagtgaaga tgaacagccg atggtcgctc atatatgggg agataagcca gaacagttcc
     3661 gtgaaacgag tattcaatta gctaaaatgg gctttaaagg catagactta aatatgggat
     3721 gtcctgtagc aaatgttgct aaaaagggta agggttccgg cttaatctta agacctgacg
     3781 ttgctgccga aattattcaa gcgactaaag caggtgggct tccggtaagt gttaaaacac
     3841 gccttggcta ctatgaaatc gatgaatgga aagattggtt gaagcacgtc ttcgaacaag
     3901 acattgccaa tttatctatt catcttcgta cacgtaaaga aatgagtaaa gtagatgcac
     3961 attgggaatt aatcgaagct attaaaaatt tacgtgacga aattgcacca aatacattgt
     4021 taacaattaa cggtgatatt cccgatagaa aaacaggact tgaactggca gaaaaatatg
     4081 gcattgatgg cgtcatgatt ggtagaggca ttttccacaa tccattcgct tttgaaaaag
     4141 aaccacgcga acacacaagc aaggaactat tagatctatt gagattgcat ttatcattgt
     4201 ttaacaaata tgaaaaagat gaaatacgac aattcaagag cttgcgtaga ttctttaaaa
     4261 tctatgtgcg tggcataaga ggcgctagcg aacttcgaca tcaattgatg aacacacaat
     4321 caattgcaga agcacgagca ctactcgatg aatttgaagc ccaaatggac gaagacgtta
     4381 aaattgaatt atagtatgag taataaagtt tatggatgat atttcccaat ttaacacgga
     4441 ttgaacactt tctacttagg tattatcttg gttttcctga taggataact cccgatgatg
     4501 cctttttact tatgtcactt atccttttgg aaatggtgca aaattaaaaa tacatcagga
     4561 tatcgtttaa ctcagaaaaa aactccaatc aaaaaaaatg tttacaacac attatgaaat
     4621 taaaattgct cttagtgttc aacagacaaa gttattgaaa ttactattgg aggtacatac
     4681 caagctatca ttttgagtac acgtggtttg aatatctggc aagaaatgga aattggtaat
     4741 gtcagtcata aagtcgctaa acgtgctcaa ataccattaa ttatttttaa ataatttaga
     4801 ggatgaggta taaaccttta aaaaacagca gtgagatgat tttcaattag aaaatatctt
     4861 actgctgttt ctattttcat aatcattctt attgaatgac ttcatcgtga tcatttaaaa
     4921 cagtaacaac tttttcaatc ttatactatt tagagctaaa aattcacgac atataaacac
     4981 a
//
LOCUS       DAMQYT010000177         4967 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMQYT010000177 DAMQYT010000000
VERSION     DAMQYT010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.180"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            101..871
                     /gene="fabI"
                     /locus_tag="PGA33_002251"
     CDS             101..871
                     /gene="fabI"
                     /locus_tag="PGA33_002251"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDG6474047.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(1066..2151)
                     /locus_tag="PGA33_002252"
     CDS             complement(1066..2151)
                     /locus_tag="PGA33_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDG6474048.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
     gene            2493..4061
                     /locus_tag="PGA33_002253"
     CDS             2493..4061
                     /locus_tag="PGA33_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDG6474049.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
     gene            4203..4961
                     /locus_tag="PGA33_002254"
     CDS             4203..4961
                     /locus_tag="PGA33_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDG6474050.1"
                     /translation="MSEFKTGKINKHVLYSNILNRDVTLSIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDEK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFDGGHQWKDWKPLLSDILLYFLSKNTDDQLYE"
BASE COUNT         1664 a          690 c          919 g         1694 t
ORIGIN      
        1 gtcataatag tgttaaaatg tattgacgaa taaaaagtta gttaaaactg ggattagata
       61 ttctatccgt taaattaatt attataagga gttatcttac atgttaaatc ttgaaaacaa
      121 aacatatgtc atcatgggaa tcgctaataa gcgtagtatt gcttttggtg tcgctaaagt
      181 tttagatcaa ttaggtgcta aattagtatt tacttaccgt aaagaacgta gccgtaaaga
      241 gcttgaaaaa ttattagaac aattaaatca accagaagcg cacttatatc aaattgatgt
      301 tcaaagcgat gaagaggtta ttaatggttt tgagcaaatt ggtaaagatg ttggcaatat
      361 tgatggtgta tatcattcaa tcgcatttgc taatatggaa gacttacgcg gacgcttttc
      421 tgaaacttca cgtgaaggct tcttgttagc tcaagacatt agttcttact cattaacaat
      481 tgtggctcat gaagctaaaa aattaatgcc agaaggtggt agcattgttg caacaacata
      541 tttaggtggc gaattcgcag ttcaaaacta taatgtgatg ggtgttgcta aagcgagctt
      601 agaagcaaat gttaaatatt tagcattaga cttaggtcca gataatattc gcgttaatgc
      661 aatttcagct ggtccaatcc gtacattaag tgcaaaaggt gtgggtggtt tcaatacaat
      721 tcttaaagaa atcgaagagc gtgcaccttt aaaacgtaat gttgatcaag tagaagtagg
      781 taaaactgcg gcttacttat taagtgattt atcaagtggc gttacaggtg aaaatattca
      841 tgtagatagc ggattccacg caattaaata atatcattca acagctttgt tcacgttatt
      901 atatatgtga gcaaagcttt tttgctttta taataatcgg actgatggaa aattatttga
      961 tatttcattt gactgatttt ttatgcacaa aaaaatcgcc tcaaaagctg tagtttcaac
     1021 ttttgatgcg atagtatctt atattcttaa aagacatcca cagaattagt ccttaacatt
     1081 actgtttgct ttatcaataa tgcgttggcg gtatttgaaa atattactaa caaccgtttt
     1141 aagtacagca tataatggca ctgcaatcag aactaatgta aagccaccta aatctcctgc
     1201 tgctaaaata acaacgataa ttgttaaagg atggatactt aaagatttac ccattacatt
     1261 tggtgtaatg atattacctt caagttgatg tgcaattaat gtaatgatac aaacccatat
     1321 aaatgtagta ggactatcta taataccgag tattgctgca ggtgcaaatg ataaccatga
     1381 acctaagaaa ggaattaagt ttgcgacacc agcaaatagt actaataaag gaatatatgg
     1441 taagtcaata attgaataac cgatatataa gaatatacct aaaataacac tgacagttac
     1501 ttgaccttga atgtaagatt ttaatgtaaa gtttaaatca gttaataaat ctacgaaaaa
     1561 tactttacgt tcacctttga aaaatttagc aacagctggg ataaattttt catggtcttt
     1621 taacatataa attaagaaga atggaaccat aatcaataag aagatggttg aaattaatga
     1681 tgtaatgtac tgtaatgaat tagataaaat attagtaacg ccatcaccca ttgatttaac
     1741 catatttgta attctacttg ttacatcttc aggtaattta tccatttgag ctagtgcgaa
     1801 tttaataatt tgctctgctt ctttttgtag ggcaggtgtc tgactaatta aattgttgat
     1861 atttgaaatg atgattggtg caacaaacgc aacaattaag ccgataatag caaacaaagc
     1921 taacatgatt gttgttatac tagcccatct tggaaaaccg actttttcaa gtaagttttg
     1981 aaaaggtaga cagatataaa ataaaaaacc actaattaaa aatggaagaa atacagaacc
     2041 gatgattgta gctattggag taaatacttc gtgcacttcc ataaatagtt tgatgagaat
     2101 gaacagcata atcagagcga tgccagttcg gaaccaaacc ttgtttaaca taaacgatgt
     2161 tcctcctatt tttctaaact tagttattat ttagtatatc ctaaatagca gtgcttaaaa
     2221 aggttaatat tttttaaata tacttaaatt aaccattcac tttctatttt aagatattta
     2281 ctttcaatag tttgtgagac ttttcaatga ctcgtaagtc tgcaattgga tcaaacagca
     2341 ccagtttata tgaataactt attttaatat taaacgtgtt taaatagtga aatgatcata
     2401 ttaatattta taagaatatt ctgaaaaata tattgaagtg tctcgtgctt aatcgtataa
     2461 tatttttatg tttaaacaat agggggaatc ttatgattga aaaattagta acctttttaa
     2521 atgaggttgt ttggagtaag ccattagttt atggtttgct aattactggt gtgctattta
     2581 cattgcgtat gcgatttttt caagttagac attttaaaga aatgattcga ttaatgtttc
     2641 aaggagagaa gtctcctaat ggtatttcaa gttttcaagc gatagccatg tctttagcag
     2701 gcagggttgg tacaggtaat attgtcggtg tatctactgc aatatttata ggaggacctg
     2761 gtgcagtatt ttggatgtgg attactgcgt ttttaggtgc aagtagtgct tttattgaat
     2821 ctacacttgg tcaaatattc aagagagttg aaaataatga ataccgtggt ggaccagcgt
     2881 attatattga atatggtatt ggtggtaaat ttggtaaaat ttacggaatt atctttgcta
     2941 ttgttacgat tatctcagta ggtctattgc ttcctggtgt gcaatctaac gctatagcaa
     3001 gttctatgca taatgcgatt catgttccac aatggttaat gggtggtatt gttgtagtta
     3061 ttttgggatt aattattttt ggtggtgtac gtagtattgc caatgttgca acagccgttg
     3121 taccatttat ggcaattatt tacatactga tggctgtcat tatcatttgt atcaatatac
     3181 aagaagtgcc agcgttattt gcattaattt tcaaatcagc atttggatta caatctgctt
     3241 ttggtggtat cgttggcgca atgatagaga ttggtgttaa acgtggatta tattcaaatg
     3301 aggctggtca aggtacaggt ccacacgcag cagcggcagc agaagtatca catccaagta
     3361 aacaaggtct agtacaagca ttttcagttt atattgatac attatttgta tgtactgcaa
     3421 ctgctctgat tatacttatt tctggtacat ataatgtgac tgatggtacg gttaatgcga
     3481 atggcacacc gcatttaatt aaagatggcg gtatttatgt tgaaaatgca acaggtaaag
     3541 attattcagg tactgcgatg tatgcacaag ccggcattga taaagcgttc catggcagtg
     3601 gttatcaatt tgatcctact ttctctggcg taggttcgta ctttattgca tttgctttat
     3661 tcttctttgc atttactaca attttgtcgt actactacat tacagaaaca aatgttgctt
     3721 atttaacgcg taatcaaaat aatcaagttt catcgatatt tattaatatt gctcgtgtga
     3781 ttattttgtt cgctacattt tacggtgcag ttaaaacagc tgatgtagca tgggcattcg
     3841 gtgatttagg tgtaggtcta atggcttggt taaatatcat tgcgatttgg attttacata
     3901 agcctgccgt aaatgcttta aaagattatg aaattcaaaa gaaacgttta ggcaacggtt
     3961 ataatgcagt ttatcaacct gatccgaata aattacctaa tgctgtcttt tggttgaaga
     4021 cgtatccaga acgtttaaaa caagcacgtg ccaaaaagta atctactttt gtttatagta
     4081 tatgtagtga tcatttgata aaaaagaaaa gtattgagaa ttttaggtgc tcagaaattt
     4141 gaattttaaa aatatagtgt ctcttggtac aataacaata caactactag gggcactttt
     4201 ttatgtcaga atttaaaact ggtaagatta ataaacatgt tttatatagt aatattttaa
     4261 atagagatgt cacgttaagt atttatttac cagaatctta taatcaactt gttaaatata
     4321 atgtcattct ttgctttgac ggattagatt ttttacgttt cgggagaata caacgtacat
     4381 atgaatcgtt aatcaaagaa gcgcgtattg atgatgcgat cattgttgga ttccattatg
     4441 aagacgttga taagcgtaga gaggaatttc atccacaagg aagtcgttct catttaacta
     4501 ttcaatcagt cggtaaagaa atattgccat ttattgactc gacgttttct acactgaaag
     4561 taggtaatgc aaggttatta gtaggggata gtttagcggg tagtattgcc ttattaacgg
     4621 cgttgaccta tccaacgatt tttagtcgtg tagcaatgtt aagtccacat tcagatgaaa
     4681 aagtattaga taagctaaat caatgtgcaa ataaagaaca attgacaatt tggcatgtca
     4741 ttggtctaga tgaaaaagat tttactttac caacaaatgg taagcgtgcc gatttcttaa
     4801 caccgaatag agaattagct gaacaaatta agaaatataa tataacttat tattacgatg
     4861 aatttgatgg tggtcaccaa tggaaagatt ggaaaccatt gctgtcagat atattattgt
     4921 attttttaag taaaaacaca gatgatcaac tttatgaata atttaca
//
LOCUS       DAMQYT010000178         4953 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMQYT010000178 DAMQYT010000000
VERSION     DAMQYT010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.181"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(304..504)
                     /locus_tag="PGA33_002255"
     CDS             complement(304..504)
                     /locus_tag="PGA33_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDG6474051.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
     gene            complement(861..1547)
                     /locus_tag="PGA33_002256"
     CDS             complement(861..1547)
                     /locus_tag="PGA33_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDG6474052.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFYFATSCL
                     VLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
     gene            complement(1888..2358)
                     /locus_tag="PGA33_002257"
     CDS             complement(1888..2358)
                     /locus_tag="PGA33_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474053.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVTESGANDTKQFTEIVS
                     EEKVITVEHAQINIFQSNSNSNLMEFNILTMGGKSGAMVGYSEIDSSHFTDRDKRVIR
                     RDHVKEAQSLVENYKDTQSADARMKAKQKVNTLSKPHQNYFNKQIDKVYNGLQR"
     gene            complement(2693..3715)
                     /gene="emp"
                     /locus_tag="PGA33_002258"
     CDS             complement(2693..3715)
                     /gene="emp"
                     /locus_tag="PGA33_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HDG6474054.1"
                     /translation="MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKKFVVPESGIN
                     KIIPAYDEFKNSPKVNVSNLTDNKNFVASEDKLNKIADSSAASKIVDKNFVVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
     gene            complement(4066..>4953)
                     /locus_tag="PGA33_002259"
     CDS             complement(4066..>4953)
                     /locus_tag="PGA33_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HDG6474055.1"
                     /translation="VNKRMLENKKEDLETIIDEFFSDIDKTRPNNIPVLEDEKQEEKN
                     HKNMAQLKSDTEAAKSDESKRSKRSKRSLNTQNHKPASQEVSEQQKAEYDKRAEERKA
                     RFLDNQKIKKTPVVSLEYDFEHKQRIDNENDKKLVVSAPTKKPTSPTTYTETTTQVPM
                     PTVERQTQQQIIYNAPKQLAGLNGESHDFTTTHQSPTTSNHTHNNVVEFEETSALPGR
                     KSGSLVGISQIDSSHLTEREKRVIKREHVREAQKLVDNYKDTHSYKDRINAQQKVNTL
                     SEGHQKRFNKQINKVYNGK"
BASE COUNT         1471 a          793 c          696 g         1993 t
ORIGIN      
        1 ttgttaaaca ttttgagtgt ttaacattac ttcttgttat cgccatatac agactttaat
       61 tctcgtcatt attaaagttc tgtattggcg cttttttata ttcaaaatca aaagtcggac
      121 agatgaaaag taaaaaactt ttccatcagt tcgatttatt ttagaaccat tatatattgt
      181 taaatctcca actaaattta ttggacaaaa aaataacggc tagtgctttt accacaccaa
      241 ctgttatcga tttaaagcat aaaaaaacag ccctatagta aggactgttt gaataagtta
      301 aaattacatt ttaactacgt ttgcagcttg ctcgccacgg tcgccttcaa cgatgtcgaa
      361 ttcaactttt tggccttctt ctaatgattt gtatccatct tcagcgattg ctgagaagtg
      421 tacgaatacg tcgctaccat cttctctttc gatgaaacca aaaccttttt ctgcattaaa
      481 ccatttaact gtaccgttat tcatattgaa tacctccgtc gtgcttttgc actaaatatt
      541 ttgtaacaaa ttcataaatg aaaaggagaa tattctatat aaataactca catttttctt
      601 cacgctcttt attacttaac tttcattata cacttttaaa tatagaagta aagcattatt
      661 ttattttctg tgataataca cattgaacta tatagtaaca ttccatttta atactaaatt
      721 taaaattttt attttctaat attaaataca cttacttttg atactattta cttttttaat
      781 tctgaattga taataaatca tatcgtgatc taaaaattat aaaagtgcca ctagcagcag
      841 tgacactttt acaatgagca ttattgacct gaatcagcgt tgtcttcgct ccaaatattt
      901 aatttctctt tttttgcttg tgcttcactt tttcttaaaa gttgttcatg tgtattgtta
      961 ggtttataaa cataagcaac tttagccaag ccttgacgaa ctaaagcttc gtttaccatt
     1021 tttccatcag cataaatata cgctaagcca cgtccatatt tatcagttct ttgacctttg
     1081 tcaaactcga cttcaatttt ctttgcattt tctaccattt ttttcgtaaa tgcacttgct
     1141 tcaggaccat atttctctac acctttttta ggatgctttg tttcaggtgt atcaaccaat
     1201 aatagtctga atgtcattgg ttgacctttg tacattaatt taaccgtatc accatcaatc
     1261 gctttaatta atgtcgcagg ttctttatgt aattttttag ttgaagttgc actatatact
     1321 gttggatctt cagaaccact tctatttacg ccattatctg tttgtgatgc atttgctgag
     1381 ctacttagac ttgaaactac aactaaagtt aacactaaac aactagtagc gaaatagaaa
     1441 aacctctttg cgtattgccc tttcgaaaca ttactgatag ccatccctat aagtaatatt
     1501 gaaacgattg ccatacatat gccagcactt aataagtatt ctgtcataac taacacctct
     1561 ttctttttag ttaattttaa tattaaacgt taattaatta taattctaca tttttacaat
     1621 taattaataa aaatttaata attataataa aataactata cttactttta tgtattttct
     1681 atattttagg tgccagaact tatatattta gccttatttg taaaattaaa aagcctacaa
     1741 catgaatcgt tgcagactat catatcgaga caaagaggtt tattagtgtc tctatatcta
     1801 tgtggaattt gaaaagatta tttataatat ttacggggat aaattgtcat ttaaaaacga
     1861 gatgtataac ttataattta ctttggatta gcgttgtaat ccattataaa ccttatcaat
     1921 ttgtttattg aaatagtttt gatgcggttt gcttaatgtg ttaacttttt gtttggcttt
     1981 catcctagca tcagcacttt gtgtatcttt atagttctct actaagcttt gtgcttcttt
     2041 aacatgatca cgtctaataa cgcgtttgtc acggtctgtg aaatgtgatg agtcaatttc
     2101 actataacca accatagctc ctgatttacc gcccattgtt aatatgttga actccatcaa
     2161 gtttgaattg ctattagatt gaaaaatatt aatttgagca tgttcaactg ttataacttt
     2221 ctcttccgat acaatttcag taaattgctt agtatcattt gcgccactct ctgttactaa
     2281 tgcgtttgcg tgattagaat gccataattg agttgcacaa attacaccca ttgttagtac
     2341 taatactttc cttttcattt gtttatgctc ccttatttgc tttgatataa aaaccgactt
     2401 tttcaccctg ttttcttcgc cattgaaaat tatataccaa tattttagaa acaataatta
     2461 acttgcgatg aacaaactat taacaataat cttgagtatt atatttatct taattaataa
     2521 tatatttatt taagattatg ttacttccaa ctttcaaagt agaaaaacgg tataatttgt
     2581 tgatgggtgt ttattgataa actgcaaaaa atacagccta caaccacata gattgtagac
     2641 tatatttaaa ataataggta tttatcatat ctcgtaaact tagtgccaat ttttatactc
     2701 gtggtgctgg taagctaccc ttaaattcag gaacgtagtg tgtagggcta taacttggaa
     2761 cagcatattg ataatttaca tttttgatat ttaatgatgg tttcccaatt ttataaccat
     2821 ttgattgtga aaatgagaaa tatttcttca cacctttaac tactttataa gaatagaagt
     2881 atttatagtc atatgcttta tttactttag cattttgatg tgttgctgtt gtgttatttt
     2941 ggaaacttgg tacatgcata cgatgtgaat tgtgaccata tggtggaatt accttgaaac
     3001 tatttatttg tggcacaaca caaaagtgat taattttaat gctagcatgc cctggtgtaa
     3061 caaatttatg cgcgtgatat ccaggaactg caaaatgatg cttgataatt aaagattgag
     3121 atggatgtgt atatctaggc gattctgatg gtttaacaat aaagtgttta ttaatagaat
     3181 cctttgcatg atttacatgt ttatgtacat gtgttgattt gtatgaagta ataactttct
     3241 tgtagtgggt ttgcgtagta atgaagtggt ggtttacttt gttttgcgta ataaatctat
     3301 ttacttctgg gcctttagca acaaaatgct tatcaacttg ttgtgaagct ggattgtttg
     3361 ttgctacatt cacgcgatta ttgatttctt tgtactctgg cacaatgttt cctaactttg
     3421 attctggtac gacaaagttt ttatctacaa ttttactagc tgccgatgaa tctgcaatct
     3481 tattcaattt atcttcagaa gctacaaagt ttttattgtc agttaaatta ctaacattta
     3541 cttttggcga attcttaaat tcatcgtaag ctggaataat tttattaatt cctgattctg
     3601 gaactacaaa ttttttgtca acactctctg tcactgatgc tttagcgtga tttgaattca
     3661 taatttgtgt agcaaatagc gtgctcatag ttaaaactaa taatttcttt ttcatctgtt
     3721 atttctcctt tatatagact caatattata accaatataa tttccctgtt atattcacta
     3781 acagcattat ataccagaat tttcagtata ataattaact tgaagtaaac gttgtcttaa
     3841 catttttatt gtttttcagc ttaaaattaa ttattgatat tgatagttaa gcataataat
     3901 tttttcgtaa tataaagtga aaaaagtaat agtccacacc tgtttagaat gtggactata
     3961 ctagattgca tcattgaaat gatgactttg atattattta ttgctagttt aaaatgttgt
     4021 tatttttacg acaaactcat tatttgcttt gcagccatgc attaattatt tgccattata
     4081 tactttattg atttgtttat taaaacgttt ttgatgacct tcacttaaag tatttacttt
     4141 ttgttgtgca tttattcggt ctttataact atgtgtatct ttataattat caactaactt
     4201 ttgagcttct ctaacgtgtt cacgcttaat tacacgcttc tcacgttcag ttagatgaga
     4261 agaatcaatt tgacttatac caaccagtga tcctgatttt ctaccaggta aagcagacgt
     4321 ttcttcaaat tcaacaacat tattatgcgt gtgatttgaa gttgttggtg attgatgcgt
     4381 tgttgtgaaa tcatgacttt caccatttaa tccagccaat tgttttggtg cattataaat
     4441 aatttgttgc tgagtttgac gctcaactgt aggcattggt acctgtgtcg ttgtttcagt
     4501 atatgtagtc ggtgatgttg gtttctttgt tggtgcagaa accacaagtt tcttgtcgtt
     4561 ttcgttgtca atacgttgtt tatgctcaaa atcatattct aatgacacta caggtgtttt
     4621 cttaattttt tgattatcca aaaatctcgc ttttctttct tctgctcttt tatcatattc
     4681 agctttttgt tgttcagaaa cttcttgaga tgcaggtttg tgattttgag tatttaaact
     4741 tcttttactt ctcttgcttc tttttgattc atcacttttt gctgcttcag tgtcagattt
     4801 taattgagcc atatttttat gatttttctc ttcttgtttt tcatcttcta aaacaggaat
     4861 attattaggt cttgttttat ctatatcact aaaaaattca tcaattatgg tttctaagtc
     4921 ttctttttta ttttctaaca tccttttgtt aac
//
LOCUS       DAMQYT010000179         4900 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMQYT010000179 DAMQYT010000000
VERSION     DAMQYT010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.182"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(76..882)
                     /gene="yjbH"
                     /locus_tag="PGA33_002260"
     CDS             complement(76..882)
                     /gene="yjbH"
                     /locus_tag="PGA33_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HDG6474056.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(905..1270)
                     /locus_tag="PGA33_002261"
     CDS             complement(905..1270)
                     /locus_tag="PGA33_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HDG6474057.1"
                     /translation="MTTTPYDIIGKEALYDMIHYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHIDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            complement(1374..1967)
                     /locus_tag="PGA33_002262"
     CDS             complement(1374..1967)
                     /locus_tag="PGA33_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDG6474058.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            2153..2500
                     /locus_tag="PGA33_002263"
     CDS             2153..2500
                     /locus_tag="PGA33_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474059.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            2517..3152
                     /locus_tag="PGA33_002264"
     CDS             2517..3152
                     /locus_tag="PGA33_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDG6474060.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            3169..3978
                     /locus_tag="PGA33_002265"
     CDS             3169..3978
                     /locus_tag="PGA33_002265"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDG6474061.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            3975..4829
                     /locus_tag="PGA33_002266"
     CDS             3975..4829
                     /locus_tag="PGA33_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDG6474062.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMTNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDRIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
BASE COUNT         1668 a          715 c          837 g         1680 t
ORIGIN      
        1 taatttaatg ttgcaaaatt aagagtcagt aaaaagacct cgttacattt atggtgtacg
       61 aggtctttaa tttatttatt ttgatttgat tttaggcatt ttagatttcc agaaatcgcc
      121 atctggatat ttaagttttt caattttttg ttgaatggct aacttcttta actctttgtt
      181 taaaagtttt tctggccatt cataaatagt aagtaattct tccatcgtta caagttgttg
      241 ttgctgtata taagtttcta atttaggagg aagattcttt tcgataggtt tacccatcaa
      301 ttcattaatt atataagtat agatgtgata tgggtataat ccttcgactt ttaaaccttc
      361 ttcatgaaca tcttcactaa agaaaacgag agaaggggct tgttcgattt ccatttctct
      421 tgcaatatgc aaatcaattt tcaagctttc ggttagttta cttttttgta agtcgtcttt
      481 aaatacttct aaatcaatac ctgcattttg aatacagtca caaatcattg attctgtaat
      541 aatatctctt ttaggtatga tttcattttg cattaaatgt ataaatcgtt cggcacgtac
      601 acgaccttgt aactcagctg ctttataagc tagggcgatg ttatcaaagt tggatgtact
      661 ttgagcttgg catttcgtta atacttttaa cgaaggattt aatatatgtc tgatacgtat
      721 atattgatta tattcaattc ttaatttgga taagattgct gataatttga agcaatcgga
      781 gctaaatgga tcgaaaaatg aataaatttc gattttactt acaggtgata gattgatatc
      841 ttcacgactc ttattttcca ttattcgtaa ttctccagcc atgtttattc acctacaatt
      901 aattttagga attcaccata tgattagcag ttaatcttaa gcgctcaaat aaataatctc
      961 caacaccttg tggaaacgca gcgcgattaa ttgctgtctg catattttct aaccatgcat
     1021 ctctttcaaa ttcagtgatt gtaaaatcta tatgtctttt tcttagcata ggatgtccgt
     1081 gttcttcggt ataaatgttt ggaccgccca aaaactgtgt taaaaattgt ttttgtttac
     1141 gacttgtttc tgcaaaatct cctggaaaca ggtgattaag tcgttcatct ttttctacaa
     1201 gggtgtaaaa ataatgaatc atatcgtata acgcttcttt accaatgatg tcatatggtg
     1261 ttgttgtcat ttaatcaccc attttcaaaa atttactgtt acgaacttaa gttaatatat
     1321 aactaatata acatgatttt aaacatttga aagaaatatg catatttgcc aatttaattt
     1381 atattgtttg aaagtgtttc ttttttcttg aaaaaacgtt gaactttatt taaaggttga
     1441 tgatgttcga ggtttagttc gtttaataaa gattggaact tttgtaaacc ttgattatag
     1501 tctttaactt cgaactctaa ctcataatcc gtagtatcga aatactcact tttatctaaa
     1561 accagtaaat cacctttata tttagtttct tggcgatatg tcgttaatgc accaagtatt
     1621 gataaagttg tatcttttac accaaactgt tcaactataa tttgacgaat gtcatctgga
     1681 agattgtcgt ttgaaataat caagttcatc tctggtttaa tgtcgacgat atagttgtat
     1741 tctaatagac caacctttgc tggtgtcttt aaagtcattt catattgatt gtctttaact
     1801 cttatgcgta gtgcagagcg atgttccttt aatttgaaat cgggtgtatc aatatagtaa
     1861 ttgacttgct taaaaagcac actgtcttta aaatatttct cttgcaattt attatagatt
     1921 gatgcagtta tcatttgttt aaattctatt tcatgatttg ttgccatgat atgtatacac
     1981 ctcgtatcaa attcaattta tcttaactat attatgaatg acaaagttga attttaaaag
     2041 taatttcctt tatctattat caatgttaat ttgaccatta aaaatagtgt tcgtaagtgt
     2101 tttgtattat tgaattgtgt taaaatgtta tggaataaga ggaggattaa gcatgcgttt
     2161 atatattaat gaaattaaaa ttaaagatga catactttat tgttatacag aagattctat
     2221 taaaggatta tctgaagtag gacaaatgct cgttgatagt gataattatg cctttgcgta
     2281 tacattagat gatggtaaag cgtatgctta tctcattttc gtacaagaaa catggacgat
     2341 gttgcatgaa aacatgacta aaaaaattat tatcaatgat gaactagaat tgactgaatt
     2401 ccaccaagaa cttacttata ttttagacaa cataaaaggg aataataatt atggtaagga
     2461 atttgttgca accgttgaag aaacattcga cattgaataa agcggggtga agcactatga
     2521 atcaatggga tcagttctta acaccttata agcaagcggt tgatgagttg aaagtgaaac
     2581 ttaaaggcat gcgcaaacaa tatgaagttg gtgaacaagc gtcgccaata gaatttgtta
     2641 ctggtcgtgt taaaccaatc gctagtatta tagataaggc aaacaaacga caaataccat
     2701 ttgataggtt aagagaagaa atgtacgata tcgctggttt aagaatgatg tgccaatttg
     2761 ttgaagatat tgatgttgtc gtcaatattt taagacaaag aaaagatttt aaagtaattg
     2821 aagaacgaga ttatattcgt aacactaaag aaagtggtta ccgctcgtat catgtcatta
     2881 ttgaatatcc aattgaaaca ttacaaggcc aaaaatttat attggctgag attcagattc
     2941 gtacattagc aatgaatttc tgggcaacga ttgaacatac tttacgatat aaatatgatg
     3001 gtgcttatcc ggatgaaatt caacatcgtt tggaaagagc ggcagaagca gcgtatttac
     3061 ttgatgaaga gatgtctgaa attaaagatg aaattcagga agctcaaaaa tattacacgc
     3121 aaaaacgttc taaaaaacat gaaaatgatt aacgaggtgt tataaatcat gcgttataca
     3181 attttaacta aaggtgactc caagtctaat gccttaaagc ataaaatgat gaactatatg
     3241 aaagattttc gcatgattga ggatagtgaa aatcctgaaa ttgttatttc agttggtggt
     3301 gacggtacat tactacaagc attccatcag tatagccaca tgttatcaaa agtggcattt
     3361 gttggagttc atacaggtca tttaggattt tatgcggatt ggttacctca tgaagttgaa
     3421 aaattaatca tcgaaattaa taattcagag tttcaggtca ttgaatatcc attgcttgaa
     3481 attattatga gatacaacga caacggctat gaaacaaggt atttagcatt aaatgaagca
     3541 acgatgaaaa ctgaaaatgg ctcaacactt gttgtggatg ttaacttaag agggaaacac
     3601 tttgagcgat ttagaggcga tggattatgt gtatcaacac cttcgggttc aacggcttat
     3661 aacaaagcgc taggtggcgc actgatacat ccttcacttg aagcaatgca aattacagaa
     3721 attgcctcga taaataatcg tgtgtttaga acggtaggat caccacttgt attaccaaag
     3781 catcatacat gtttaatatc accagttaat catgatacca ttagaatgac gatagatcat
     3841 gttagtatca aacataaaaa tgttaattca atacaatacc gtgtagcaaa tgaaaaagtg
     3901 aggtttgcac gttttagacc attcccattc tggaaacgtg tgcacgattc tttcatatca
     3961 agtgatgaag aacgatgaaa tttaagtatc atatatcaca acaagaaact gttaaaactt
     4021 ttttagcacg acatgatttt tctaagaaga cagtgagcgc cattaaaaat aatggcgctt
     4081 taattgttaa tgatgaacca gtcacagtgc gtaagcaatt aatgacaaat gatatattag
     4141 aaattcattt accgcgagaa ataccgagtg ttaatttaat accttatgct cgtaagctag
     4201 aagtattgta tgaagatgct tttatcatca tagttactaa accaaacaat caaaattgta
     4261 cgccttcgag agaacatcct catgaaagtt taatcgaaca agtactatat cattgtcagg
     4321 aacatggtga aaatattaac ccacatattg ttacgcgtct agatcgtaat acaactggta
     4381 ttgtgatatt cgctaaatat ggacatatcc atcatttatt ttctaaagta aacttgaaaa
     4441 aaatatatac ttgccttgta tatggtaaaa cccatacatc tggtattatt gaagctaata
     4501 ttagacggtc aaaggatagg attataacta gagaagttgc ctcggatggt aaatacgcta
     4561 aaacatctta tgaagtaata aatcagaatg ataaatacag tttatgcaaa gttcatttgc
     4621 atacgggacg tacacatcaa attcgtgtac attttcaaca tattgggcat ccaattgtgg
     4681 gagattcttt gtatgatggt tttcatgaca aaattcatgg tcaagtactg caatgtacgc
     4741 aaatatattt tgttcatcca atcaataaga acaatattta tattacaatt gattataagc
     4801 aattacttaa attattcaat caactctaat tcacacaggg ggtgtaagta tgtcaatgaa
     4861 cacagatgaa aaagagcgtg ttcaagagga attatacgac
//
LOCUS       DAMQYT010000180         4858 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMQYT010000180 DAMQYT010000000
VERSION     DAMQYT010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.183"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(1..813)
                     /locus_tag="PGA33_002267"
                     /pseudo
     CDS             complement(1..813)
                     /locus_tag="PGA33_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
     gene            complement(934..1653)
                     /gene="mecA"
                     /locus_tag="PGA33_002268"
     CDS             complement(934..1653)
                     /gene="mecA"
                     /locus_tag="PGA33_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDG6474063.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVYFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
     gene            complement(2024..2419)
                     /gene="spxA"
                     /locus_tag="PGA33_002269"
     CDS             complement(2024..2419)
                     /gene="spxA"
                     /locus_tag="PGA33_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDG6474064.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
     gene            2714..3703
                     /gene="trpS"
                     /locus_tag="PGA33_002270"
     CDS             2714..3703
                     /gene="trpS"
                     /locus_tag="PGA33_002270"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDG6474065.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            complement(3745..4626)
                     /locus_tag="PGA33_002271"
     CDS             complement(3745..4626)
                     /locus_tag="PGA33_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6474066.1"
                     /translation="MQNKSKSPFKIAFSKFIHNKIAMLSVIFLLIITIVSIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGIKDAFNPRGRR"
     gene            complement(4638..>4858)
                     /locus_tag="PGA33_002272"
     CDS             complement(4638..>4858)
                     /locus_tag="PGA33_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDG6474067.1"
                     /translation="LGADIVSILGGAVITETIFSYNGIGKLFLESVIGQDYPLMMALT
                     LFFSFLGLLGNLISDITYGFIDPRIRSN"
BASE COUNT         1689 a          823 c          668 g         1678 t
ORIGIN      
        1 caacttgtaa ttgccactca ataggatgat ttttaatata aatttgctct ggaaaaatat
       61 aaccataatt gtgcactact tcttgatcag ttaactgtaa ttgatacatt gcacttaaag
      121 tgggttctaa aacagaattt tgccagcgac aatatttgat atattgatta attgcgaact
      181 ttgataattt ataaacagca tgataatcac aaggcgcctc attaaacaac tcactaattt
      241 tggcatttct tttttcagcg acatatttgt gcccgcctaa atattgtatt tgttgaaaca
      301 tcattatttg ttttttctct aaattgaacg tatgaagttt tcgagtgaat ggattaataa
      361 acatactttg aaaatgattg aacttcactt tattatgaca ataatcaaca tcatttaata
      421 accaacttac tttatatccc attttcttta aaccttccgt tcgttgaaga atctgtttat
      481 aaggaattgg cgaatactgt agctcaataa catatttatt attaatcaaa atatccggaa
      541 tttgttttat ttcttttaaa atggctctat ctctacttta catccttgtt gctgtatcat
      601 ctgtgctaaa atcaatttac ttttataatg tttaatcgtt tcattattaa aacatttttg
      661 ttccgctaaa tgtttatgtg caaaatgact aattactttg agcccacgct ttaaaataac
      721 ttgcttgcca cacaccggac aaaaatattg tgtctttctc aatgcagtag ttgctaatac
      781 gcgttccttt tcttcattta aagctactaa catatcttca cctcaaaata ttacacgtaa
      841 gtttaactac tattactttt tcagcttttg aaatcaataa aaaaagagac tccaaagagt
      901 ctcctttcat agtgcgttgc ttaattaata tcgttattca gttgtctctg gaaaataacg
      961 tcgaacttga gctgttacgt tatgactcat aattatttta gcatagtcat ttaaataaac
     1021 ttctgttctg tctgttggat aagcaaattc aagcaattga ctgtaactat cattaatgac
     1081 ttcttgatca acatgactat caaaatatac agcataataa taagtaccat caaccatata
     1141 taacaaatct tcaaactctg tagttattgg attgctatga tatgcataat taataacatc
     1201 ttctaaatcg ttaaatttca caatgattgt tcttgtattt ttacgtgctg aagacttttg
     1261 acgtttagaa ccttgagctt ctttttcttt tgttcgttgc tcgaataatt cttctaattg
     1321 atcttcacct tctaatgttt gagctaacaa ttcttgaact tgttcatcaa attgatcagt
     1381 tgcatcatca tcagacatat tcatcatatc ttcattttta gatttagaaa ttgtgacttc
     1441 gacacctttt tcaaaggcat gtacttgaat ccataatgga ccttctacaa caaaatcttc
     1501 ttcttcgtta atttcatcca tcattgacca aaagaattct tcgccacgtt tgcgatttgt
     1561 ccataaatct tcacgactaa atccacgggc ctcgatatcg ctatatgtta taaacaattt
     1621 tacagttgta tcatctactc gttctattct catatcatct cactccttac agtcgatgaa
     1681 tagtatccac attgtattaa agcatcaaac aaaatacaat ttatttgttt gatttctttc
     1741 tctaaaatat tttggtaact tataataaca tattcgttgt agcaaaacta tttaaatgat
     1801 ttatacagtt aaagcattaa agcactttct taatttaatt ttaccttatc atagcaaatg
     1861 tttcatccaa aacactctaa tatagagcta tattaacaat tgcacatttg atttaaaaga
     1921 tactaagcaa ttattgtaaa attaaaacca aaaaagcaac actatagaaa tagtgttgct
     1981 tgacatatct tttagtaaag tatgacacat agttaaatgg ttattagtca accatacgtt
     2041 gtgcttcttg taattggaac gtacgaactt ttctaggtaa gaaacgtcga atctcgtcct
     2101 cattataacc aacttgtagt cgtttattat ctaaaataat tggacgacgt aataagccag
     2161 gattatcttg aatgattgaa tataagtctt gtaatggtag tgaatcaata tcaacattta
     2221 atttttggta tgttttagaa cgtgtagaaa tgatttcatc agtaccgtct tcagtcattt
     2281 ttaatatttg cttaatttca tcaattgtta aatgttcaga aaaaatatta cgctccgtat
     2341 acggaatgtc atgttcttgt aaccatgctt tcgctttacg gcaagatgtg caacttggtg
     2401 aagtaaataa tgttaccata catctcactc tcctatttga atgaataaaa ttcattgctt
     2461 aaaatttagt tatagatcaa gaaaaaacat ttttttctaa aattcttaat cgttactatt
     2521 tattataact atctaacatt aaaattaaat gagaaaaacc taatttttca gataagtttc
     2581 tacacttata aaaaagatta ttaatccctt tgttagtagt aagttatacg tatattctaa
     2641 cacatcttac atttttaaga aatactgtta taatgataat tattaaaata ttactaagaa
     2701 agtaggcatt taaatggaga cattattttc aggcatccaa cctagtggaa ttcctactat
     2761 tggaaattat attggcgcac taaaacaatt tgttgatgtg caaaatgact atgattgtta
     2821 tttctgtatc gtagatcaac atgcaattac aatgccacaa gatcgtttaa aattacgtaa
     2881 acagaccaga caattagcag cgatttattt agcttctggt atagatccag acaaagcaac
     2941 attgttcata caatctgaag tccctgcaca cgtacaagca ggatggatgt taactacgat
     3001 tgcttctgtt ggagaattag agcgtatgac gcaatacaaa gataaagctc agaaagcagt
     3061 tgaaggtata cctgctggtc tattaacata tccaccttta atggcagctg atattgttct
     3121 ttacaatact aatatcgttc cagttggaga tgaccaaaag cagcatatcg aattgactcg
     3181 taaccttgta gatagattta atagtcgcta taatgatgtg cttgtgaaac ctgaaattcg
     3241 tatgcctaaa gttggtggac gtgtcatgag tttacaagac ccaacaagaa aaatgagtaa
     3301 gagtgatgat aatgctaaaa acttcatttc attattagac gagccgaatg ttgcagctaa
     3361 aaaaattaaa agcgcagtaa ctgattcaga tggtattatt aaatttgatc gtgacaacaa
     3421 gccaggtata acaaatttaa tttcaatata cgctggatta acagacatgc caattaaaga
     3481 tattgaggca aaatatgagg gcgaaggtta tggtaaattt aaaggtgacc ttgctgaaat
     3541 agttaaagca tttttagtag aattccaaga aaaatacgaa agtttctata actcagataa
     3601 acttgatgat attttagatc aaggtagaga taaagcacac aaagtttcat ttaaaactgt
     3661 caaaaaaatg gaaaaagcga tgggtttagg acgtaagaga taaaataaag aggtactaga
     3721 attaactagt gcctctttta ttatttaacg tctacctcta ggattaaaag catctttaat
     3781 cccttcccct acaaagttta tacttaatat agttaatgta atcattatag caggtggcat
     3841 ccatatccac ggtttaccac ttataacatc cccttcttga gcatcactca acatattacc
     3901 ccaagatggt attgctttac taattccgaa tcctaaaaag ctcaatcctg attccactac
     3961 aatcataccg gcaaataaca atgttgcttg tacgataact acacttaata tattcggcaa
     4021 aagatgtttc aaaataattt tatatgtggg tgtaccaatt gattttgctg ccaaaaagta
     4081 ttcattttct ttttcttgaa gtactttacc acgaacaagt cttgcaatac ctccccaact
     4141 taatacgata ataactagaa caagaattat ggcagatcca taaggatttt taattttatc
     4201 tccaagtgca gcatttaata caattgcaaa tattaaaaat ggaaataaca taacaaattc
     4261 ggttattctc attaataatg tatcaacaat acctccaaaa taaccagaaa tcactccaac
     4321 tgtaattcca atcaaaagca ttcctattgt agatgtaatt ccaatggata atgaaatacg
     4381 acctgcatat aacaaacgac taaagttatc cctaccacca gagtctgtac caagaatgtt
     4441 ttgtgctgtc atttcacctt ttatatttaa taaatcttgt tggttcactg gaaaaggagc
     4501 tattaatggc gctataattg atacaatagt tatgattaat aaaaaaataa ccgataacat
     4561 tgcaatttta ttatgaataa atttagaaaa tgcaatttta aaaggcgatt ttgacttatt
     4621 ttgcattcta atccccccta gttacttcta attcttggat ctataaatcc ataagtaata
     4681 tcagaaatca aattacccag taaacctaaa aatgagaaaa ataacgttaa tgccatcatt
     4741 aatggatagt cttggcctat taccgattct aaaaataatt taccgatacc gttatatgaa
     4801 aagatagtct ccgtaatcac ggctccacct aaaatactta caatatcagc acctaaaa
//
LOCUS       DAMQYT010000181         4857 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMQYT010000181 DAMQYT010000000
VERSION     DAMQYT010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.184"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(1..1169)
                     /gene="sqr"
                     /locus_tag="PGA33_002273"
                     /pseudo
     CDS             complement(1..1169)
                     /gene="sqr"
                     /locus_tag="PGA33_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
     gene            complement(1188..2522)
                     /gene="cstB"
                     /locus_tag="PGA33_002274"
     CDS             complement(1188..2522)
                     /gene="cstB"
                     /locus_tag="PGA33_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HDG6474068.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYRLPKSGISTQSVHSADMTGKEEHVLDVRNDEEWNNGHLDQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            complement(2553..3620)
                     /gene="cstA"
                     /locus_tag="PGA33_002275"
     CDS             complement(2553..3620)
                     /gene="cstA"
                     /locus_tag="PGA33_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HDG6474069.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEEENDS
                     YDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
                     IKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAA
                     LIIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPKKNIRMPLSK
                     MNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEV
                     EYGGVGTYIGATENANHNLFI"
     gene            3757..4017
                     /gene="cstR"
                     /locus_tag="PGA33_002276"
     CDS             3757..4017
                     /gene="cstR"
                     /locus_tag="PGA33_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HDG6474070.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMGIIISENLIECVKAAADDEESSQELINEAVNLLVKSK"
     gene            4017..4772
                     /locus_tag="PGA33_002277"
     CDS             4017..4772
                     /locus_tag="PGA33_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDG6474071.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIF
                     APIGLKLSKKVPNIIQKWIISILIVFAIVQLIL"
BASE COUNT         1494 a          869 c          696 g         1798 t
ORIGIN      
        1 taccaataca ttttaggtaa taaatctttc ttaaatatat acatacttct acgttctttg
       61 gcctgattaa acggcattgt ttctttagta tttttattat aatcaaactc tgcaagtatt
      121 aacctattat atccagtaac aatagggcat gaagtataac catcataatg atgcgttaac
      181 atttgattat tcatcacttg caataaatta ttagcgacga taggtgcttg cttacgaata
      241 gctgcgcctg tttttgaagt aggtacattt gaagcatcac caagtgcaaa tacattagag
      301 tagcttttat gctgtaatgt ggttgggtta acatctaccc aaccctcact atctgaaagt
      361 gtactttctt ttactacatc taagggaccc ataggtggtg ttacatgtaa catatcataa
      421 cttattgttt ttctatcgta tgctttgata tgttcgaatg tagccacttt tttgtcaccg
      481 tcgatttcaa caaggttata attataattg actgttatat ttctttcttc aacaatcctt
      541 tctaattctt tattatattt tcctacgtca aataaagcat cttttggcgt tgcatatatc
      601 acattagcgt tagaacggat tttatgtttc ctaaaataat cttcagctaa atacataatt
      661 ttcataggcg cacctccaca ctttatagga gtgtttggat gcgtaaaaat ggcatttcct
      721 tgtttaaaat tagaaatttg gttccaagtt tcgttaacat agtcaggtga atagttagag
      781 caaacaccat ttttacctat attttctttt agtcctttaa ttgaagacca attaatctgt
      841 aatcctggag ctactactaa aaaatcataa taaacgactg tattatctcc taaaataacg
      901 ctattatttt caggttaaaa acttgaaaca gcctgtttta tccagttagc accttcaggt
      961 ataacacttt ccatatcttt acgagaactt ttcaaactag atacccctgc accaaccaac
     1021 gtccataatg gttgatagta atgatggtct gctggatcta taattgctat tttctctttt
     1081 aagttttgat tttttcttaa tagtcttgat gctacggtaa cacctgctgt accgccacca
     1141 ataataacaa tttgataatg cttattcatt ttttccacat ccttaaatta ttttaatgat
     1201 tctggaaaat cttgatagcc ttctctaata ttcaccacat tttcaaaacc tttgctttcc
     1261 aatataccca ctgcaattga acttctaaca cctgactgac aatgtacata tattttatcc
     1321 tctttattaa aaggaatatt ttcatttaat aatttaccat gcggaatatt aactgcttga
     1381 tctaagtgtc cattattcca ctcttcatca ttacgtacgt ctaatacatg ttcttcttta
     1441 cctgtcatat cagcgctatg aacggactgg gttgaaatgc ctgattttgg caaacgatag
     1501 cctgctacct tatcaaaccc aattaattgt aaagtgtgtt tcgctttctc aacagtagat
     1561 ttatctccaa ttaaatctat atctttttca aaatctaagt accaaccaat ttgattaata
     1621 aagtttttgt tgtatggtat attgattgtt ccttttgtgt ggccaccgtg aaaggcctct
     1681 ttgctacgaa gatcaaatgc tactctctta ttatctaaac taggataaac atcatatgat
     1741 tgatataaat tcataccaaa ctgattaact tgtttcattt gtgcaaaatg atgcggtggt
     1801 gctggttgat ttgatgttaa tgattcaata aatttagtct catcaatttc attaaatgcc
     1861 cagttattaa ttttctcata acctattgta gatataggta tggcacctaa tgctttacca
     1921 caagggcttc cagcaccatg acccggccag atttgaacat agtctggtaa atttttaata
     1981 ttttgaacgg actcatacat ttgtttcgcg ctaatttctg tagaaccctt tatttgaaca
     2041 gatttttcta ataaatcagg tctacctata tcaccaacaa aaataaagtc accactaaat
     2101 aatcccatcg gaacacttga accaccacct aaatcagtga gtaaaaagct aatactttca
     2161 ggcgtgtgtc ctggagtatg cagaacttct aatttaacat tgcctacttg aatgatatct
     2221 ccatgtttaa caaattgtgt ttttgatggc atatttttat accctaatgc atcttcacct
     2281 tcgccagaca catatatatt tgcatttaag cgtttagcca catcacgaat tcctgaagca
     2341 aaatcagcat gaatatgtgt ttctgtagct tgtgtaattg ttaaaccttc agaatctgca
     2401 acttctatat atttcgataa atcacgaaca gggtctatta ttattgcctc tcctgtacgt
     2461 tgacaaccca ctaaatatga tgcttgagat aaatgattat cgtaaaactg tttaaaaaac
     2521 atgataacaa ctccttttat taatagattt aattagataa ataaattatg attcgcattt
     2581 tcagtagcac caatataagt gcctacacca ccgtactcaa cttcatctct aagttcttct
     2641 ttctgaatac ccatgacatc catactcatc gtacaagcga ttaatttgat attttgctca
     2701 atagcttgat tgataagtgt tggtaatgaa tcaacatttt tctttttcat tacgtagcgc
     2761 atcatcatat ttcctaaacc aaacatattc attttggaaa gaggcattcg tatattcttt
     2821 ttgggcaaca ttaaatcaaa catttttgca ataccttgct ttttaacatt aactgtttgc
     2881 acttttttta atgcattaag cccccaaaaa gtaaagaaga tagttacatc ttttccagca
     2941 gctctagcac catttgcaat aatcaacgct gctacagctt tgtctaattc tccactaaat
     3001 aatacaattg tagtaccttt agcagaataa tttatatcta aatcttttgc tttttctttt
     3061 tgaataatcg cattaattcc attgttattt tcatcaagct taactaaagt atgccttgtt
     3121 tgtttcaccc aacttttaat gtcactaggg aatccagggt ctgtgacttt gacttcaatt
     3181 tggtcaccta cttcaatatt cttcatttct tgactaattt ttacaattgg ccctggacat
     3241 tgaagaccac gatagttaaa ttgtttacga tctgctttaa tttctatact tttatattct
     3301 tcttgtgtat cataactatc gttttcttct tcataagctt tataaccacc atcaagattt
     3361 ataacgttat aaccttgttt agctaaatat ttactagcat tagcacttct gttaccactt
     3421 ctacagatta cataataggt tttatttttt tctttattga atgactcaat ctcatcaaca
     3481 ggatgaagta tggaaccatt gatatgtcct aatgcatact cctcttctgt tctaacgtca
     3541 attaattgcc cttgcttggc tagtttttcc aattctgctt tactaaattc atcgataaac
     3601 ttttctccgt attgtttcat aataacctcc ctaaaaatac ctgtaggggt attttaaaca
     3661 gttggatgga acttgtcaaa tacctctagg ggtatttgac acatttgtgt ttatctatta
     3721 atatattttt attcaattaa aagtggaggt atgactatga attatgataa aaaaatgatt
     3781 aatcgtatta atagaataca agggcaacta aatggaatta ttaaaatgat ggaggaagga
     3841 aaagactgta aagatgtcat tacacaaata agtgcatcaa agagttcact ccaacgcttg
     3901 atgggtatca ttattagtga gaatttaata gaatgtgtaa aagcagctgc ggatgatgaa
     3961 gaaagctccc aagagttaat taatgaagct gtaaacttat tggtgaaaag taagtaatgg
     4021 atattgttaa tatagttatc atgttactca ttggggtatt cggtgggttc atatctggtc
     4081 tagtaggcgt aggtggtgct atcattattt atccagctat tttattatta ccaccattat
     4141 tcggagcccc tgcatatagc gcatatatcg catcaggtct tacttctagc caagtctttt
     4201 tcagtacatt aagtggctca ttaaaagctc gaaaaaaaac agagttctcg ccacaattag
     4261 ttctttatat gggtggcggc atgattatag ggagtatgtt aggtgcattt ctagcaaatt
     4321 tatttgatgc tacatttgta aatacggtat atatcattat tgctttactt gcattaacat
     4381 taatgtttat taaagtgaaa cctagttccg aaaagtcttc atttaataaa tatttattag
     4441 ttatcattgg ccttttcatt ggtattattt caggtatagt aggtgcaggt ggtgccttca
     4501 ttattattcc tatattattg gttttattca aattaccaat gaatactgta gtcgctaata
     4561 gtatagtaat tgcttttata tcttcaattg gtgcttttgt catcaaactt attcaaggtt
     4621 atatcccttt atatgacgcc ttattcctta taatagggag tattatattt gctccaatag
     4681 ggttaaaatt aagtaaaaaa gtccctaaca taatacaaaa atggattatc agtattttaa
     4741 tcgtttttgc tatagttcaa ctcattttat aaaactaatc cttttaactc agacagcaat
     4801 ttcccgaaaa ataatacctc ttatttaaac aaatatgtat gcagttcgga tttatat
//
LOCUS       DAMQYT010000182         4846 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMQYT010000182 DAMQYT010000000
VERSION     DAMQYT010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.185"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            22..168
                     /locus_tag="PGA33_002278"
     CDS             22..168
                     /locus_tag="PGA33_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474072.1"
                     /translation="MMLTAIYFSIFTFYISQYSLKLKTLYNIETDYNYKIVLLLKEGK
                     THES"
     gene            158..682
                     /locus_tag="PGA33_002279"
     CDS             158..682
                     /locus_tag="PGA33_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDG6474073.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFCKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRNLRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     regulatory      669..766
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            841..1932
                     /gene="gcvT"
                     /locus_tag="PGA33_002280"
     CDS             841..1932
                     /gene="gcvT"
                     /locus_tag="PGA33_002280"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDG6474074.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            1952..3298
                     /gene="gcvPA"
                     /locus_tag="PGA33_002281"
     CDS             1952..3298
                     /gene="gcvPA"
                     /locus_tag="PGA33_002281"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDG6474075.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            3291..4763
                     /gene="gcvPB"
                     /locus_tag="PGA33_002282"
     CDS             3291..4763
                     /gene="gcvPB"
                     /locus_tag="PGA33_002282"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDG6474076.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSKHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
BASE COUNT         1746 a          641 c          943 g         1516 t
ORIGIN      
        1 ggatatgtta cgccgttctt attgatgcta acagcaattt atttttccat tttcactttt
       61 tatatcagtc aatatagttt aaaattaaag acattatata atattgaaac tgactataac
      121 tataagattg ttttgttatt gaaagaaggg aaaacgcatg aatcatgata aatcaccaat
      181 aattttaatt ggtttcatgg gtaccggtaa atctacgatt ggtaaatacg ttgcagatga
      241 gcaaaattta tcatttattg atatagattc atatatcgaa gagaagtata agttaacaat
      301 accagaaata ttttgtaaac atggtgaaca atatttcagg aatttagagt tcacatgttt
      361 gcaagaatgt attaacactg cagatataat tgctactggt ggtggtatta ttgagagtga
      421 agaggcattt aattttttga aaaatcaaaa aaacattatt tggttagatt gtaatattga
      481 tattatatat agtcgaatca atgatgaccc acatcgacct aatgcaaata ataagacaat
      541 caagcagtta aatgacttgt attgctcgcg gaatttaaga tataatgaaa tcgcattcaa
      601 gaaatttgat agtcatttgc tatcaatttc agaaatatat tatgaattgc taaatttaat
      661 aaaagcgagt gatcagtatt agagagaata gagcgttaag actctatcgc cgaaggtgca
      721 agtaatttat tacgaaactc tcaggcaaaa ggataatact gtaacgcgtt cctgaattgg
      781 tgatttataa acagggtagc gattgctatc ctgtttttat aattttaagg gggtatttca
      841 atgtcaagtg atttaaaaca aacaccttta tatcaaaatt atgttgatag aggtgcaaaa
      901 attgtggaat tcggaggatg ggcgatgcct gttcaatttt caagtattaa agaggagcat
      961 aatgctgttc gatacgaaat tggcctgttt gatgttagtc atatgggtga aattgaagta
     1021 acaggtaaag atgctagtca gtttgtgcaa tatttattat caaatgatac tgataattta
     1081 actacttcaa aagcattata tactgcttta tgtaatgaag aaggcggtat tattgatgat
     1141 ttagtaatat ataaattagc tgacgacaat tatttattag ttgttaatgc tgctaatact
     1201 gaaaaagatt ttaattggat tttaaaacac aaagagaaat ttgatgttga agtacaaaat
     1261 gtatcaaacc aatatggtca attagcaata caaggaccaa aagctagaga tttaattaat
     1321 caattagttg atgaagatgt aactgaaatg aaaatgtttg aatttaaaca gggtgtcaaa
     1381 ttatttggag caaacgtcat tttatctcag tcaggttaca caggtgaaga tgggtttgaa
     1441 atttattgta atatcgatga tactgaaaaa atttgggatg gtttattaga gtataatgtt
     1501 atgccatgtg ggttaggcgc tcgtgataca ttaagattag aggctggatt gccattacat
     1561 ggacaagatt taactgaatc aattacacca tatgaaggtg gtatcgcttt tgcaagtaaa
     1621 ccattaattg atgctgattt tattggtaaa tctgtattaa aagatcaaaa agaaaatggt
     1681 gcaccaagaa gaacagtggg attagaatta cttgaaaaag gaattgcaag aactggttat
     1741 gaagttatgg atttagatgg aaatattatt ggagaagtaa cttcaggaac acagtctcca
     1801 tcatcaggaa aatcaattgc acttgcaatg ataaaaagag atgagtttga aatgggtaga
     1861 gagttgcttg ttcaagttcg taagcgtcaa ttaaaagcga aaattgttaa gaaaaatcaa
     1921 attgataaat aattaaaaag gggtgtgcat tgtgagtcat cgttatatac ctttaactga
     1981 aaaagacaag caagaaatgt tacaaacaat tggtgcaaaa tctataggag aattattcgg
     2041 tgatgtacca agtgacattt tattaaatag agatttaaat attgctgaag gcgaagcgga
     2101 aacaacgtta cttagaagat taaatcgcat tgcaagcaag aatatcacta aagaaacgca
     2161 tacatcattt ttgggtgctg gtgtttatga tcattacgcg ccatcagttg tggatgctat
     2221 gatttcaaga tcagaatttt acacagcata tacgccatat caaccggaaa tttctcaagg
     2281 tgaattacaa gccatttttg agttccaaac tttaatttgt gaattaacag atatggatgt
     2341 agcaaattct tctatgtatg atgggatgac tagctttgct gaagcatgta tcttagcatt
     2401 tagtcaaaca aagaaaaata aaattgtagt ctcaaaaggt ttacattatc aagcattaca
     2461 agtacttcat acttatgcta agacgcgtaa ggaattcgaa gtagtggaaa tcgatttaga
     2521 tggcactgtt acagatttga aaaaattaga agcagcagtg gatgatgaaa cagcagctgt
     2581 tgcagtacaa tatccaaact tttacggttc aattgaagat cttgaaaaga ttcatagctt
     2641 tattgaagat aaaaaagcat tatttatcgt atatgcaaat ccattagcat tgggattact
     2701 tacacctcca ggttcatttg gtgcagatat tgttgtaggt gatactcagc cattcggaat
     2761 tcctgctcaa ttcggtgggc cacattgtgg atattttgca acgacgaaaa agttaatgcg
     2821 taaagtgcca ggtagattag tcggtcaaac acaagatgat gaaggtaatc gtggctttgt
     2881 attaacatta caagcacgtg aacaacatat tcgacgcgat aaagcgacat ctaatatttg
     2941 ttctaaccaa gcattaaatg ctttagcgtc atcaattgca atgtcagcac ttggtaaaca
     3001 aggtatctat gatattgcag ttcaaaatat agaacatgct aactatgcta aacaacaatt
     3061 tattaaaaaa ggttttgaag tattagatgg tacatctttc aatgagtttg tagtcaaatt
     3121 tgacaaacca attcaacaag ttaacgagga attagtgaag tacaatatta tcggtggttt
     3181 tgatttaggt gttgtttcag acgactttaa aaatcacatg ttaatagctg taactgaatt
     3241 aagaacaaaa gatgaaatcg atacatttgt agaaaaggct ggtgagttaa atgactagta
     3301 aatcaagtcc attaattttt gaaagatctc gtgaaggcag atatgcatat tcattaccaa
     3361 aaagtgatat taaaacaaat tctgttgagt cattgttaga tgataaattt attcgtaaaa
     3421 acaaagcgga gtttcctgaa gttgctgaac tagacttagt acgtcattat actgaacttt
     3481 caaataaaaa tttcggtgta gacaacggat tttatccatt gggttcatgt acgatgaagt
     3541 ataaccctaa aattaatgaa aaggttgcta gaataccagg atttagtgaa tcacatccat
     3601 tacaagatga agatcaagtt caaggttcat tagaaattat ttatagtttg caagaagaat
     3661 taaaagaaat tactggtatg gatgaggtga cattacaacc agctgctggc gcacatggtg
     3721 aatggactgc attgatgata tttaaagctt accatgagaa taatggtgaa ggtcatcgtg
     3781 atgaagtcat tgtgccagat tctgcgcatg gtacgaatcc agcctcagct tcatttgcag
     3841 gatttaaatc agttactgta aaatcaaacg aacgtggcga agttgatatt gatgacttga
     3901 aacgtgttgt aaatgaaaat acagcagcta ttatgttaac taatccaaac actttaggta
     3961 ttttcgaaaa aaatattatg gaaatccgtg aaatcgtcca taatgctggt ggtctattat
     4021 attatgatgg tgcgaattta aacgctatta tggacaaagt tcgcccagga gatatgggat
     4081 ttgatgctgt tcatttaaac ttgcataaaa catttactgg tccacatggt ggtggcggtc
     4141 ctggttcagg tccagtcggt gtagtaaaag aactagcaag ttacttacca aagccaatgg
     4201 ttattaaaga tggcgacaaa tttaaatatg ataatgacat taaaaattct atcggacgtg
     4261 taaaaccatt ttatggtaac tttggtattt acttaagagc ttatacgtat attcgaacta
     4321 tgggagcaac tggacttaaa gaggtttctg aagcagcggt tcttaatgcg aattatatta
     4381 aagcacgttt atctaaacac tttgaaatac cttataaaca atattgtaaa cacgagtttg
     4441 tgttaagtgg tgtgcgtcaa aaagaatttg gtgtacgtac tttagacatg gctaagcgat
     4501 tattagattt cggtgtacat ccaccaacaa tatacttccc attaaatgtt gaagaaggta
     4561 tgatgattga accgactgag acagagtcta aagaaacact tgattatttt atcgatacat
     4621 taattagtat tgctgaagaa gctaaaaatg atcctgataa agtgctagaa gcaccacata
     4681 caactgtgat tgatcgatta gacgaagcta cagctgctcg taaaccaata ttaaagtttg
     4741 aaaatcttaa acaggaaaaa taaagtatta aacacatatt ccgagaatta ttattctaac
     4801 tttgtatgaa gatttaagga taatggtttc aaaatcaatt gaaaaa
//
LOCUS       DAMQYT010000183         4819 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMQYT010000183 DAMQYT010000000
VERSION     DAMQYT010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.186"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            7..888
                     /locus_tag="PGA33_002283"
     CDS             7..888
                     /locus_tag="PGA33_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDG6474077.1"
                     /translation="MNQVMNIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVEENIDNVDKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAIEDTRVILRISAETIPGEGFAGARIIRKEVQKMFLQEGI
                     KTPQPIMTAYNHSENGV"
     gene            918..1121
                     /locus_tag="PGA33_002284"
     CDS             918..1121
                     /locus_tag="PGA33_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDG6474078.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIESHDDTQR"
     gene            1133..2230
                     /gene="ychF"
                     /locus_tag="PGA33_002285"
     CDS             1133..2230
                     /gene="ychF"
                     /locus_tag="PGA33_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDG6474079.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(2316..2507)
                     /locus_tag="PGA33_002286"
     CDS             complement(2316..2507)
                     /locus_tag="PGA33_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474080.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            2751..3047
                     /locus_tag="PGA33_002287"
     CDS             2751..3047
                     /locus_tag="PGA33_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDG6474081.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            3068..3571
                     /gene="ssb"
                     /locus_tag="PGA33_002288"
     CDS             3068..3571
                     /gene="ssb"
                     /locus_tag="PGA33_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDG6474082.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            3623..3865
                     /gene="rpsR"
                     /locus_tag="PGA33_002289"
     CDS             3623..3865
                     /gene="rpsR"
                     /locus_tag="PGA33_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDG6474083.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(4131..>4819)
                     /locus_tag="PGA33_002290"
     CDS             complement(4131..>4819)
                     /locus_tag="PGA33_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDG6474084.1"
                     /translation="LKYLVLKLITENNQEDGYKIIDEFLCIDKSYSNSNFDTNSRTPE
                     EVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQNPPAWVCIEFMQLGQFVSFLNFYYKK
                     YNDEELRVANILMPLVKNIRNKSAHNQPIIANLNYDSRLPQYLFEKGNNIGISRNMFG
                     IKNFIDTFATLELHNQVCSNAIIQARYHDLDQLQKRYKRNESYYNNALAIKRFFIALD
                     KIIDFNRPKV"
BASE COUNT         1700 a          672 c          910 g         1537 t
ORIGIN      
        1 cttatcatga atcaagtcat gaatattatt tcatctctat ttgagccatt aacaaaaata
       61 gaaacatatg aaaacattgc aactaaaatc gctatgattg ttatttatat tatcgtagcc
      121 ctcatagtta ttaaaatact gaataaaatg attgaacagg gatttaagat tcaaaataaa
      181 agtaaaaaga gtaacaaaaa gcgctctaaa actttaatat ctcttgttca aaatgtagtg
      241 aagtatatcg tttggtttat agttattacg acgattttaa gtaaatttgg cattagtgtt
      301 gaaggggtaa ttgccagtgc tggtgtcgta ggcttagcag taggttttgg tgctcaaact
      361 atagttaaag acgtaattac aggattcttt attatatttg aaagccaatt tgatgtaggt
      421 gattatgtta agataaataa cggtggtaca actgtagcag agggaacagt taaatcaatt
      481 ggacttcgtt caacacgaat caatacaatt tcaggagaat taacaatttt accaaatagt
      541 agtatgggtg aaataacgaa ctactcaatt acaaatggta cagctatcgt taaaattcca
      601 gtgtctgtcg aagaaaacat tgataatgtt gataaaaaac taaacaaact atttacttct
      661 ttacgtagta aatattactt atttgttagt gatccggttg ttattggtat tgatgctatt
      721 gaagatacaa gagtaatatt gagaatatct gcagaaacaa ttccaggtga aggatttgct
      781 ggagctcgaa ttattcgcaa agaagtacaa aaaatgtttt tacaagaagg tattaaaaca
      841 cctcaaccaa ttatgactgc ttataatcat agtgaaaacg gtgtttagta gtttataata
      901 catggaggtc atatttaatg gcgtcaaaat atggaataaa tgatatagta gaaatgaaaa
      961 aacaacatgc gtgtggaaca aaccgtttta agattattag aatgggtgca gacataagaa
     1021 ttaaatgtga aaattgtcaa agaagtatta tgattccacg tcaaacgttt gataaaaaac
     1081 ttaaaaaaat catcgaatct catgatgata cacaaagata ggagaatgat taatggcttt
     1141 aacagcaggt atcgttggat tgccaaacgt tggtaaatca acattattta atgcaataac
     1201 aaaagcaggt gctttagcag cgaactatcc attcgctacg attgatccta atgtagggat
     1261 agtagaagtg ccagatgcta gattacttaa attagaagaa atggttcaac ctaaaaagac
     1321 attgccgact acatttgaat ttacagatat cgctggtatt gtgaaaggtg cttcaaaggg
     1381 agaagggtta ggtaataaat tcttatcaca tattagagaa gtagatgcga tttgtcaggt
     1441 cgttcgtgca tttgatgatg ataacgtaac tcatgttgct ggtcgagtag accctattga
     1501 tgatattgaa gttattaata tggaattagt actagcggac ttagaatctg ttgagaaacg
     1561 tttgcctaga attgaaaaat tagcacgtca aaaagataag actgctgaaa tggaagtacg
     1621 tattttaaca actattaaag aagctttaga aaatggtaaa cccgctcgta gtattgactt
     1681 taatgaagaa gatcaaaaat gggtgaatca agcgcaatta ctgacttcta aaaaaatgct
     1741 ttatatcgct aatgttggtg aagatgaaat tggtgatgat gataatgata aagtaaaagc
     1801 gattcgtgaa tatgcagcgc aagaagactc tgaagtgatt gttattagtg caaaaattga
     1861 agaagaaatt gctacattag atgatgaaga taaagaaatg ttcttagaag atttaggtat
     1921 cgaagaacca ggattagatc gattaattag aacaacttat gaattattag gattatcaac
     1981 atattttact gctggtgtgc aagaagtacg tgcttggaca tttaaacaag gtatgactgc
     2041 acctcaatgt gctggtatca ttcatactga ttttgaacgt ggatttatcc gtgccgaagt
     2101 aacaagttat gacgactatg tacaatatgg tggcgaaagt ggcgctaaag aagcgggcag
     2161 acaacgatta gaaggtaaag aatatattat gcaagatggc gatatcgttc atttcagatt
     2221 taatgtataa acgatagagt gaagttaatt aaatagtata tatgtagaag aggcggaatc
     2281 aattgttcgc ctcttttaat tatgcgtata atttattaaa agaatggaat aattttactc
     2341 gcgttaataa tatcttgagt gctgaaaaat tgtttgcctt cgccagtata agcaggctct
     2401 aaaacaagat tagcctttgc acaataaagc cattcaggat gaataccact attaagtatc
     2461 tcttggaatt cttgaaaatc tttagaccaa tcaatattta aattcattcc ttaccacctc
     2521 acaactatta tagaacatat gttcgctttt gtgaagtgta tttttaaaaa tatcatagaa
     2581 agtttcaaat gaattaatat caaaaatgat atgaagagta gtttgaatta ttattgtaaa
     2641 aagtaatggc gcatgatata attctttatt gtgagtaatg aaaattattc cttgcttatc
     2701 tgttttaaga ttgataagcc gtatagacca caaggaggtg caaatataaa atgagaacat
     2761 atgaagttat gtacatcgta cgcccaaaca ttgaggaaga tgctaaaaaa gcgttagttg
     2821 aacgtttcaa cggtatctta gctactgaag gtgcagaagt tttagaagca aaagactggg
     2881 gtaaacgtcg cctagcttat gaaatcaatg atttcaaaga tggcttctac aacatcgtac
     2941 gtgttaaatc tgataacaac aaagctactg acgaattcca acgtctagct aaaatcagtg
     3001 acgatatcat tcgttacatg gttattcgtg aagacgaaga caagtaataa ttagaggggg
     3061 cgtttaaatg ctaaatagag ttgtattagt aggtcgttta acgaaagatc cggaatacag
     3121 aaccactccc tcaggtgtga gtgtagcgac attcactctt gcagtaaatc gtacgttcac
     3181 gaatgctcaa ggggagcgcg aagcagattt tattaactgt gttgttttta gaagacaagc
     3241 agataatgta aataactatt tatctaaagg tagtttagct ggtgtagatg gtcgcttaca
     3301 atcccgtaat tatgaaaatc aagaaggtcg tcgtgtgttt gttactgaag ttgtgtgtga
     3361 tagcgttcaa ttccttgaac ctaaaaatgc gcaacaaaat ggtggccaac gtcaacaaaa
     3421 tgaattccaa gattacggtc aaggattcgg tggtcaacaa tcaggacaaa acaattcgta
     3481 caataattca tcaaacacga aacaatctga taatccattt gcaaatgcaa acggaccgat
     3541 tgatataagt gatgatgact taccattcta ataaaaatta acgaaattaa agcgaaaaaa
     3601 ttatcaaagg aggcacacaa tcatggcagg tggaccaaga agaggcggac gtcgtcgtaa
     3661 aaaagtatgc tatttcacag caaatggtat tacacatatc gactacaaag acactgaatt
     3721 attaaaacgt tttatctcag aacgcggtaa aattttacca cgtcgtgtaa ctggtacttc
     3781 agctaaatat caacgtatgt tgactacagc tatcaaacgt tctcgtcata tggcattatt
     3841 accatatgtt aaagaagaac aataatatat aatttattgt caaaccccgt aggcataggc
     3901 ttacggggct ttttgtgttt tggggtatag aaaaagggca aaaaaggatg atgtgaatgt
     3961 tttgtgttcg gaatttgcac aaagatatgt ttatattgca aaaataatat gaatttagat
     4021 gcataaaaaa agaactacgc attttaaata aaatgcatag ctcttctttt tcttgcatac
     4081 gaattaaaat aactcgcgag acctataagt ctctttcctc actagatagt ttatactttt
     4141 ggtctgttga agtcaataat tttatctaaa gctataaaaa atcttttgat agctaatgca
     4201 ttattataat agctttcgtt tcttttatat cgcttttgaa gttggtccaa atcgtgatat
     4261 cttgcttgga taattgcatt actacaaact tgattatgta attctagcgt agcgaaagta
     4321 tctatgaaat tttttattcc gaacatgttt ctagatatgc ctatattatt ccctttttca
     4381 aataaatatt gaggtaatct actgtcataa tttagatttg ctatgatggg ttggttatga
     4441 gctgatttgt ttcttatatt tttaactaaa ggcattaaaa tattagcaac tctcaattct
     4501 tcgtcattgt acttcttgta atagaagttg agaaacgaaa cgaattgacc tagttgcatg
     4561 aattcaatgc aaacccatgc gggtggattt tgatagtatt tattcaactt ctcgggtagt
     4621 tgtcctcgtt tattcatatg cttgaatatt tcgtttttat ttttgatttt ggtttccata
     4681 acttcttctg gtgttcttga atttgtgtca aaatttgaat tgctatatga tttatcaata
     4741 cataagaact catctattat tttataacca tcttcttggt tattttctgt tattagtttt
     4801 aagactagat actttaaac
//
LOCUS       DAMQYT010000184         4808 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMQYT010000184 DAMQYT010000000
VERSION     DAMQYT010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.187"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            272..1159
                     /locus_tag="PGA33_002291"
     CDS             272..1159
                     /locus_tag="PGA33_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDG6474085.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSNDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKKGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            1307..2257
                     /locus_tag="PGA33_002292"
     CDS             1307..2257
                     /locus_tag="PGA33_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDG6474086.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVTGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            2312..3031
                     /locus_tag="PGA33_002293"
     CDS             2312..3031
                     /locus_tag="PGA33_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDG6474087.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            3015..3815
                     /locus_tag="PGA33_002294"
     CDS             3015..3815
                     /locus_tag="PGA33_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDG6474088.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLTLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTAAFINKHHDEKHV"
     gene            3951..4457
                     /locus_tag="PGA33_002295"
     CDS             3951..4457
                     /locus_tag="PGA33_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474089.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            4619..>4808
                     /locus_tag="PGA33_002296"
     CDS             4619..>4808
                     /locus_tag="PGA33_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474090.1"
                     /translation="MRINDKILLENIEDYFNHKGLSPHLIDDIKEKVITDIKNSEKKD
                     QDYIEYKRKSPAQIILMIQ"
BASE COUNT         1790 a          625 c          888 g         1505 t
ORIGIN      
        1 acagttgttt gtttgtgcag caactgcata agagccccta atcgctaaag ctcaagggga
       61 gtaaaggaat acagttgttt gtgcagcaac tgcataaaag cctctaatca ctaaaggtga
      121 agaggaacgc agttggatgc taaggcacaa ctgcataaaa gcctctaatc gctaaagatg
      181 aagaggaacg cagttggatg ctaccgcaca actgcataaa tccctctaat cgctaaagcg
      241 aaaagtggga ttaaaaagga gatgtgatag tgtgaagaaa tcgttaattg cttttatttt
      301 gatttttatg cttgtcctga gtggctgtgg tatgaaagat aatgataaac aaggtagcaa
      361 tgataatggc tcgtctaaat cgccgtacca tagaattgtt tcgttaatgc ctagtaatac
      421 tgaaatttta tatgaattag gattaggtaa atacatagtt ggtgtttcaa cggttgatga
      481 ttatccaaaa gatgtgaaaa agggtaagaa acaatttgat gctttgaatc taaataaaga
      541 ggaactttta aaggcaaagc cagatctaat tcttgcgcat gagtcgcaaa aggcaactgc
      601 taataaagta ttgtcatcat tagagaaaca aggcatcaaa gtagtgtatg ttaaagatgc
      661 acaatcaatt gatgaaactt acaacacatt taagcaaatt gggaaattaa cgcatcatga
      721 taagcaggct gaacaacttg ttgaggaaac taaagataat atcgataaag tcatagattc
      781 aattcctgct catcataaaa aatcaaaagt atttattgag gtttcatcaa agcctgaaat
      841 atatacagca gggaagcata cattttttaa tgatatgtta gaaaaattag aagcccaaaa
      901 tgtgtatagt gacattaatg gttggaaccc tgtaacgaag gaaagtatta ttaaaaagaa
      961 cccagatata ttaatttcga cggaagctaa gacaagatca gattatatgg atatcatcaa
     1021 aaaaagaggt ggattcaata aaattaatgc tgtcaagaat acacgtattg aagttgtaaa
     1081 tggtgatgaa gtatcaagac caggtccacg tattgatgaa ggattaaaag aattaagaga
     1141 tgcaatttat agaaaataaa ccattctaat tatgcccctt attgctacat gtaaaaaata
     1201 catgtttgag ataaggggtt tttaaaatat atttagtgaa tgatagcaac gcgagtatgt
     1261 gattgctata atgaatgtaa ttatcgatga aagaaaagag aatgctatga catttaataa
     1321 agtattattg agctggatag tcatattgat tataacaact agcatatatc tattttggca
     1381 gttgggcgat atcaatgatg tatttaacca gtctatttta atcaatgtta gattaccgag
     1441 attattagaa gcattgttga caggtatgat attaactgtt gcaggcctta tatttcaaac
     1501 agttttaaat aatgcattgg cagatagctt tacattagga ttggcaagcg gcgctacatt
     1561 tggttcagga ttagcattat ttttaggttt aacaacgtta tggattcctg tattttcaat
     1621 aacatttagt ttgataacat taataactgt attagtcatt acgtcggtat tgagccaagg
     1681 ctatccagtt agaatcttaa tattaagtgg tttaatgatt ggtgcgttat tcaattcact
     1741 tctatatttt ttgattttat taaaacctcg caaattaaat acaattgcca attatctgtt
     1801 tggtggtttt ggtgatgcag aatactcaaa tgtatctata atagcaatca catttatcat
     1861 tgcattgttt ggtatattta tcattcttaa tcaactaaag ttattgcaat taggagaact
     1921 aaaaagtcag tcactaggct taaatgttca attgattaca tatatcgcgt tatgtatagc
     1981 ttctatgata acggcgataa atgtcgcata tgttggcatc attggattca ttggtatggt
     2041 gataccgcaa ctcattagaa aatggcagtg gaaacaatca ttaggaagac aattggcttt
     2101 aaatattgta actggaggac aaataatggt tatggcagat tttattggta gccatatatt
     2161 gtcaccagta caaataccgg caagtattat cattgcatta attggtatac cagtgttatt
     2221 ttacatgcta atatctcagt cgaaacggtt acactagcac acgacatttg ctaaaataaa
     2281 aataactata aacataaaga gggcataagc gatggatttg aatcaaatta aagcagttgt
     2341 atttgattta gaaggtacgt tgttggacag agttaaatct cgagagaaat ttatcgaaga
     2401 gcaatatgaa cgatttcatg actacttaat tcatgttcaa ctggcagatt ttaaaaaagc
     2461 atttattgag ctagatgacg atgaagataa tgataaacct gatttatata aagaaatcat
     2521 taaacgtttc catgtagata ggttaacttg gaaagactta tttaatgatt ttgaaatgca
     2581 tttttatcgt tatgtatttc cttattacga tactttgtat acactagaaa agctatcgca
     2641 aaaaggcttt caaattggtg ttatcgcaaa tggtaaatct aagattaaac aatttcgatt
     2701 acattcactt ggtttgatgc atgttattaa ttatttatca acatcagaaa cagttggttt
     2761 tcgtaaacca catcctaaaa tttttgaaga tatgattgat caactagggg tattacctga
     2821 gcaaattatg tatgttggcg atgatgcgtt aaatgatgta gctccagcac gagctatggg
     2881 catggttagt gtatggtata aacaagaaga tgctgaaatt gaaccactcg aagaagaagt
     2941 tgattttaca attacaacag tggaagaatt attaaccatt ttaccaataa aaaatgataa
     3001 taaaggagaa aattatggat ctatttacta gaaaagatgg aacatcgata cattacagta
     3061 cattaggtga aggctatcct atcgtattga ttcatactgt acttgataat tattctgtgt
     3121 ttaataaatt agcagcagaa ttagcaaaat catttcaagt tgtgttaatt gatttacgtg
     3181 gacatggcta ttctgataaa cctcgtcaca ttgaaataaa agatttttct gatgacattg
     3241 ttgaattact taaatattta tatattgaag aagttgcatt tgtatgccat gaaatgggtg
     3301 gaatcattgg tgcggatatt tcagtacgtt atcctgaatt tacatcatca cttacgttgg
     3361 taaatccaac atctattgaa ggtgaattac cggaagaacg tttatttaga aaatatgccc
     3421 atattattcg aaactgggat cctgaaaaac aagataaatt tttaaataag cgtaagtatt
     3481 atcgtccgag aaaaatgaat cgattcctca aacatgtcgt agatacaaat gaaatatcaa
     3541 ctaaagaaga aattcaagca gttaaagagg tattcaaaaa cgctgatatt tctcaaactt
     3601 atagaaatgt cgtagtaccg acaaaaatta ttgcaggaga attcggtgaa agaacaacaa
     3661 gattagaagc taaagaagta gctgatttaa tccaaaatgc ggactttgaa gtatatcaag
     3721 aatcaagtgc attcccattt gttgaagagc aagaaagatt cgtcgaagat acagctgcat
     3781 ttatcaacaa acatcacgat gaaaagcatg tttaattttt agtgataagt gaaatataaa
     3841 agtagtaatt attatttgta atataattgt aatatgactg ttgttttaga aatgattgtt
     3901 gtataatggt tgtgagcaca agacgatata aataaaagga gagagtgctt atgaaaaagc
     3961 tactaacggc aagtataatt gcatgttctg ttgtaatggg agtaggctta gtgaacacta
     4021 gtgccgaagc agcaagtggc aactctattg atactgttaa acaattaatt aagggtgatc
     4081 agtcattaga aaatgtgaaa attggcgaat ctattaaaga tgttttaact aagtacaaaa
     4141 atccgatgta ttcttacaat gaagatggaa ctgaacatta ttacgaattc catactaaaa
     4201 aaggtatgtt attagtaact actgatggta agaaaaacaa tggtaaagta actcatattt
     4261 caatgatgta taatgatgct aatggtccaa catatcaagc tgttaaaaat tatgttggca
     4321 aagcagtaac acatacggaa tatagcaaag ttgctggtaa ttttggatat attgaaaaag
     4381 gcaaaacgac ttatcaattt gcctcagcac caaaagataa aaacataaaa ttatatcgta
     4441 ttgatttgga aaaataataa ttaaattaat cgtgcttaac cgacaagtta aatcgcaata
     4501 cttgtcggtt attttattac atggcataat acagtgaata ttaattttta tgatttgcga
     4561 aggggaaatt aaaccaatac aataaataaa gatgtttcat tttaaaggag tactaccaat
     4621 gcgtattaac gataaaattt tactagaaaa tatagaagat tactttaatc ataaaggttt
     4681 atcaccgcat ttgattgatg atattaaaga gaaagtaatt actgatataa aaaattctga
     4741 aaagaaagat caagattata ttgaatataa aagaaaatct ccagcacaaa tcatattaat
     4801 gattcaaa
//
LOCUS       DAMQYT010000185         4749 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMQYT010000185 DAMQYT010000000
VERSION     DAMQYT010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.189"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            334..1299
                     /locus_tag="PGA33_002297"
     CDS             334..1299
                     /locus_tag="PGA33_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDG6474091.1"
                     /translation="MSISEYKNRYLESLYNTFKTELENERTRYFILDTNYTNSDDTEK
                     EFTWDVKQNNKIREGDLFIFRRPTNLSQIAQQFYFFGAGKIEKIERKNNVATAYISKP
                     LLFVDRVLKDNIENLKWEFKERIKEEWEQFFFKNKISNITKNDFLKLLSLSRNVVEIE
                     SNYINNDISLTFKQMADMATRIKKNIYYVGNHEAFVQAKGSAHFEFSKRIKQNYSYRC
                     AITGIKTKDFLVVTHIIPWHENEFIRLDPSNGICLSLFLAKAFKKGFITFSNSYRVVL
                     SKEAEKDAALYEELKIYENQKIELPDCQKPNLKYLDWHREHIFKN"
     gene            1396..1587
                     /locus_tag="PGA33_002298"
     CDS             1396..1587
                     /locus_tag="PGA33_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474092.1"
                     /translation="MYYLAYGLTTLDNCFKYYQLITQNEHFCYSQQEERESYQQKFQS
                     MINGLKIKIGIQSSNDDAQ"
     gene            1679..2008
                     /locus_tag="PGA33_002299"
     CDS             1679..2008
                     /locus_tag="PGA33_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474093.1"
                     /translation="MARTYNVIKYVIKKNKDDLKIHGLRLYEFNIKDEKAINISQPIF
                     VNRATLIEKIQNGEAFTHAFRVNHNTYYAGQLISLSLNKNGYINNNFEASRDIIRNIE
                     VSILDYE"
     gene            complement(2085..2444)
                     /locus_tag="PGA33_002300"
     CDS             complement(2085..2444)
                     /locus_tag="PGA33_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002434158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474094.1"
                     /translation="MENTNSNMHKNDNSYVLSALSYLSIFFAPVILPLFVWILADEPT
                     SEHGKKAFINHIMTWVSFFIGRLAFIFSKEVFDKPLDHQLLIFSILLIITIIFFLIAL
                     ILYILNINRGIKILLKK"
     gene            complement(2974..3824)
                     /locus_tag="PGA33_002301"
                     /pseudo
     CDS             complement(2974..3824)
                     /locus_tag="PGA33_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4337..>4749)
                     /locus_tag="PGA33_002302"
     CDS             complement(4337..>4749)
                     /locus_tag="PGA33_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6474095.1"
                     /translation="EPYESLTELQHLFDKHLLDESERRFIVEDENQVVGIVELVEINY
                     IHRNCEIQIIIKPEFSGKGYAKFAFEKAIIYAFNILNMHKIYLYVDADNKKAIHIYES
                     QGFKTEGLLKEQFYTKGKYKDAYFMSLLKSEYIL"
BASE COUNT         1770 a          606 c          684 g         1689 t
ORIGIN      
        1 attattttat tttataaaaa tagtttaata aaatgtttaa tttttataaa aaataattaa
       61 atattataga cattacgata gtttaattag aaaatatatt taagaattat gaatttagtt
      121 cttattaatg ttaaagatca tcaattattt tgtcacatta tcttgagagg attaccttat
      181 gaggtagcct tttatttgtc acctaatatt gctctaaggc taataataca tatataaaat
      241 aaataattat cagaatatat ttagaaattg atatttaata attaaataaa agtatgctaa
      301 atatttataa tttacaagtg agctgataga aagatgagta taagcgaata taaaaataga
      361 tacttagagt cactatataa cacctttaaa actgagttag aaaacgaacg cactcgctat
      421 tttatattag ataccaacta tacaaatagt gatgatactg aaaaggagtt tacttgggat
      481 gtaaaacaga ataataagat cagagaaggc gatttgttca tttttagacg gccaactaat
      541 ttatctcaaa ttgctcaaca attttatttc tttggagctg ggaaaataga aaaaattgag
      601 cgaaaaaata atgtagcaac cgcttacatt tcaaaaccct tactttttgt ggatcgtgtt
      661 ttaaaagata atattgaaaa tttaaaatgg gagttcaaag aaagaattaa agaagagtgg
      721 gagcagtttt tctttaaaaa taaaatcagt aatattacaa aaaatgattt tttaaaacta
      781 ctaagtttga gtagaaatgt agtggaaata gaaagtaatt atattaataa tgatatttcg
      841 ctaactttta aacaaatggc tgatatggca actcgaataa agaaaaatat ttactacgta
      901 gggaatcatg aagcatttgt tcaagctaag ggaagtgctc attttgagtt ttcaaaaagg
      961 attaaacaaa attatagtta tagatgtgca attaccggga ttaaaactaa agacttttta
     1021 gtcgttacac atattattcc ttggcatgaa aatgagttta ttagactaga tccttcaaat
     1081 ggtatttgtt tgtctttatt tttagcaaaa gcatttaaga aaggctttat cactttttct
     1141 aacagttata gagttgtttt atctaaagaa gcagagaaag atgctgcttt atatgaagaa
     1201 ttaaagattt atgaaaatca aaaaatcgaa ctaccagatt gtcagaaacc taatttgaaa
     1261 tatttagatt ggcatagaga acatattttt aaaaattaat tttgggagag gttgtatgac
     1321 agatattagt caattagtag aggtgccata catctttcat tagtagattg aaaaagagca
     1381 cttgaaacag ataatttgta ttatttagcc tatggattaa caactttaga taattgtttt
     1441 aaatattacc aattaattac acaaaatgaa catttttgct attctcaaca agaagaaaga
     1501 gaaagttatc agcaaaaatt tcaaagtatg attaatggtt taaaaattaa aattggcata
     1561 caaagttcaa atgatgatgc gcaataagct acaaagtttg attttttaat tgaacgttat
     1621 atgtttttaa aagaagaaag agcttcgaat taatatctta atggaggaag attagtaaat
     1681 ggctagaacg tataatgtga ttaaatatgt cattaagaaa aataaggatg atcttaaaat
     1741 acatggatta agattatatg aatttaatat aaaagatgaa aaagctataa atattagcca
     1801 acccattttt gtgaacagag caacattgat tgagaaaata caaaatggtg aagcatttac
     1861 gcacgccttt agagtaaatc acaatactta ttatgcaggc caattaattt ctttatcatt
     1921 gaataaaaat ggttatatta ataacaactt tgaggcctca cgagatatta ttagaaacat
     1981 tgaagtaagt attttagact atgaataaag gtaatactga tagaatacat tacaattaaa
     2041 aaagggagta taggactccc ttaataagtt gatatataag tgacttattt ttttaataga
     2101 attttaattc ctctgttaat gttcaagata tataatatta aagcaattaa aaagaatata
     2161 atagtaataa ttaaaagaat gctaaatatt aataattgat gatccaaagg tttatcaaag
     2221 acttctttag aaaaaataaa tgccaatcta cctataaaaa aactaaccca agtcataata
     2281 tgattaatga atgctttttt gccatgttct gatgttggtt catcagcaag tatccataca
     2341 aataaaggta aaatgacagg tgcgaaaaag atacttaaat aactcaatgc tgataaaacg
     2401 taactattgt catttttatg catattacta tttgtatttt ccactttttt caccttcttt
     2461 acttttcttt actattataa aaacattaat agcgagctat tacaacagtt ataagagaag
     2521 cttacgatat tgtaagtatg tgttgttata atcaatataa aattaagaaa gacctgagaa
     2581 tttatattat catccacaat cgaagtgagt agcgaaattt attggaaaag gtacttatat
     2641 tgaagatatt gttaatggtt catttttaaa aagccaagac gatttgaagt taaaagcttc
     2701 tgacttcact gtcgaagata actataacta cggtgtactt atatttccag agaatctttc
     2761 tcttacagat aataataaga aattggaatc tttaataaaa actatactat ttacaggtaa
     2821 acgatatcaa tatacttatt ttaaaatttg atcttaatag ataaggatat aatgatttaa
     2881 acaatttaag agatattatt ttcacaaaaa aagaatgctt tttgttagag gcactgaacc
     2941 cttaaaatgg gaacttaata aaaacactat gttctaggct gctaattctc tgtattttac
     3001 aggggataag tagcctagtt tttgttgaat tcgattatta ttatagtttt taatgtattt
     3061 tcgacaatat ctattacaat atgattagag ttattaagct gattattgat gtaaaaagtt
     3121 tcagacttta gcgaggaatg gaaactttct atcggggcgt tatcggcagg tgttcccttt
     3181 cgggacatac ttctgataat gcctttttct tcgcataatt gataataagc ataagatgta
     3241 taaacgctgc cttgatcact atgtaatata cacccctcag gtatatcgat ttgatttaat
     3301 gtatcattaa ctaaacgttg gtcttgttta tcatctattt tatacgccac aatttctccg
     3361 ttataaatgt ccattatcga agataaatac aacatagaat gaccaaatgg taaataagta
     3421 atatcggttg ttaatacttc catgggacaa ctcgctttaa attgtctttg taataaattg
     3481 tccgttttat aatacggttt acctattctt gtcgtctttt taggtctaac tcggcagttc
     3541 aaatgatgct tctgcatcac tctctgtact ctcttatgat taattggtga tgtataacat
     3601 tgattaatca atgctgtaat cttacgataa ccgtaggtat attgtttacc aacatgttgt
     3661 gaaaatctat aactttcccc atttcgatac catcgccacc aatttttcac ttgtgttcta
     3721 tttctaatat ttaattcttt cataatttct tttgttggaa atcctgctgc tttcatttca
     3781 actgctttat actttgtttc tactgaataa gaagctcttt tcatagaaaa aaaacacctc
     3841 cgtatgattc attttaatat gaattcaacg aaagtgtttt tatataattc ccactattgg
     3901 ggtcagtcta aaattaaaat tgataacttt tttaataaaa tagatacact aattttaaaa
     3961 caaggtaaaa aaatagaatt attaaaacaa cgcaaacaag gcttactaca aaaaatgttt
     4021 gtgtaattct gataacgtcc tattatgtta attgaaaata gacaaaaata aattgagagc
     4081 atcccttacc gcaaagtgaa ggatgctcta tttttgttga aattaatata agcttcattc
     4141 tgatagatag tcattaataa ttgtttgcaa atcgttatta agtaccgttg ttacagcgtc
     4201 atcattcata aaatcctcgt agattttatc aagaatttat tcctcttaca gacattcgcg
     4261 agaagtccgt tttaatttta ttagaagtaa ttcaggtttg aacctaccta aatgaatata
     4321 tgagttatct tttatactac aaaatatatt cagatttcaa taatgacata aaataggcat
     4381 ctttatattt acctttagtg tagaattgct ctttaagtaa tccttctgtt ttaaatcctt
     4441 gtgactcgta tatatgaata gcttttttgt tatctgcatc aacatataga tagatcttgt
     4501 gcatatttaa aatattgaat gcataaatta tcgctttttc gaatgcgaat tttgcataac
     4561 ctttaccact gaactcaggt ttaataatta tttgtatttc acaattacga tggatgtaat
     4621 taatttctac taattcaaca atacctacga cttgattttc atcttcaaca ataaaacgtc
     4681 tttccgattc atctaataaa tgcttatcaa ataaatgttg aagttccgtt aaggattcat
     4741 aaggctctt
//
LOCUS       DAMQYT010000186         4748 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMQYT010000186 DAMQYT010000000
VERSION     DAMQYT010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.190"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(203..724)
                     /locus_tag="PGA33_002303"
     CDS             complement(203..724)
                     /locus_tag="PGA33_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HDG6474096.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(752..2047)
                     /locus_tag="PGA33_002304"
     CDS             complement(752..2047)
                     /locus_tag="PGA33_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDG6474097.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVAPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(2087..2563)
                     /locus_tag="PGA33_002305"
     CDS             complement(2087..2563)
                     /locus_tag="PGA33_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6474098.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(2732..3268)
                     /locus_tag="PGA33_002306"
     CDS             complement(2732..3268)
                     /locus_tag="PGA33_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDG6474099.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            complement(3479..4417)
                     /locus_tag="PGA33_002307"
     CDS             complement(3479..4417)
                     /locus_tag="PGA33_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDG6474100.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDNNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVVENIKAADIVLSDDEIQYIDELFPIED"
BASE COUNT         1515 a          891 c          634 g         1708 t
ORIGIN      
        1 taattcacaa agtatatagc tatgatcaaa aaaatgtgtc agttattcaa attttttatt
       61 aactacagat taatttaatt tcaaaaaaat taaaaaaact cccaatcaat ctaagacatt
      121 attttcacct cacgatttac gtcgtcattg agaaatgaat catatcttta tagattaatt
      181 gggaaatttt agtttctaat atttaattaa atgcttgatg taaagcatca gaagaatgct
      241 tccacatacc ttcatcatgg ttcattaaaa agtcttttaa aatagcttta ttctcgtcac
      301 caatttgatc tatgataata tgatgtttca ttgatttatc catcatatta acatgttcag
      361 gcatactttt ataaccacga cgaattgctt tattcactaa taaatagcac gctgttaaac
      421 cagcgtaata tggtccttgt tcattacgtt ctgttgttac ccaacacaac caatattctt
      481 tagcttcatc gccttcaaca gcagctttat cagtaatcca tttcacacct ttttcaactt
      541 tggcacgagc atgcatgcct ccaatatcaa taaatgcttc tttgttagca acatcgataa
      601 aaactggtgc aatattgtct aagctaattg aaccaatatt tgtaccttta tgaccatcta
      661 gagggtcatt ttttatgata ttaaaattaa agcctttttt ctcagacaat gatataacct
      721 ccatatttaa cgataacaat tttagccctg tttaattaag ctcattaatc aaattttcaa
      781 tagcttctct aattgtctta tctttaatct ttgcaaccat ttctctagga taatataatt
      841 tatagtcttg gcgattaatg cctgtaatac tatgaatgac taatgattga ttgcttattt
      901 ctctaatcgt accatcttgt cttaataaat gaatcggttt gcgatttgac cccggtcgat
      961 catagtcata tggcaaatca gaaaatgctt cactcacaaa ataataatct ggattaatac
     1021 cacctgcttc aaacagttct tgcagttctg atattgtaat aattgagcca tcaaatggaa
     1081 tatatttaaa taagtctcga ttaataaatc gacttgctaa atcacttaaa atagcatcat
     1141 cttcttttgt ccatttttcc aaataatacg taactaccgc ttcatcgagt tcaacatatt
     1201 gttcaatcgt aactgtctct tcaaaaaatg gaataaaatc atgtggatgc aacttaaatt
     1261 catagccttc attataaagc tgttttgcgc gtttcaaaca attattaagc agcacttctc
     1321 caccacgact aactgggtgg aagtaaattt gccaatacat ttgataacga ctcataataa
     1381 agttttcaac tgcatgcata ccactttctt tgattagtac ttcatcttta gaaggtcgca
     1441 ttaatcttaa aatacgctcc atatcaaaag caccatatga tacacctgtg aaatacgcat
     1501 cacgttgtaa ataatccatt ctatccgcat cgatttgtga cgaaatcatc gaaatgacca
     1561 atttattatg atgcgtttta ttaattactt ccgcaacttc tcttggaaac tcaggcgcca
     1621 ctttacgtaa tacagcattc acctcagtat ctccagtaat aatcgcttgt gtgtatgctt
     1681 catggtctgt attaaatatt ttttcaaaac tatgtgaaaa tggaccatgt cctaaatcat
     1741 gtaataatgc agcacataat gccaacggtc tatctttatt gtcccatgca tcatgaccaa
     1801 taaatgactc atcaattaat cgtctaacta tttcatacac acctaaagaa tgtccaaagc
     1861 gactatgttc tgctgtgtga aaagataggt acagtgttcc tagttgtcta attcgacgta
     1921 acctttggaa ttccttagtt ttaattaaat cccatatcaa ttgatcttca acatgaatat
     1981 atcgatgtat cgggtcttta aaaacttttt cttctactaa ttttaaatct acatatgcgt
     2041 tagtcattat tcccctcctt ttcgtttaat ataatattta atttacttaa aatgctttgt
     2101 acataagtgc taagtctaac ttttcgccat acatttctgg ctcataagag cgtaagattg
     2161 taaaaccttg ctctttatag taagctactg cttcttcatt tttattatct acttctaagt
     2221 aaacaccttc aaatttatct tcaaaacgtg ataatccttc atttaacaat gctgtaccat
     2281 aacctgtatg ttgcgattct ggtttaacat aatgagctga taaatataat tcttcaccgt
     2341 aaataaagtt agcaaagcca acgatgtcat taccttcttc aacgactaag aataattgtt
     2401 cttgaagtct tttctttaaa tgatgttcat tatatgaagc ttctaacaag tgattaactg
     2461 ttgtcgcagc gtatatattt aagtatgtat taaaccaagc tttagttgcg acatctctaa
     2521 tttgaacaac atctttttca gttgcttgtc ttaccttgaa catgactttc tccccttatt
     2581 aacaagtttt aataacggca ttataccaca acttgctcaa tacttaataa acaatgattg
     2641 tctattcaat ttatatattt atattttccg ttaaaattaa aaataaaaaa taacgaagca
     2701 aaaaatcact tcgtttagta tgaggtatgt cttattgcaa tatactattc cactcagttg
     2761 cacgtgctaa ggcatagttg tctttcatga tgtcaccagg cttttcagca gttccaataa
     2821 tataaccatt taaagtggca cctaaaaagt ctaaactata tttcatttgc gtaattgctg
     2881 gcttcgcttt tatttttgga caatctccac caactaaaat aactctaaaa tccttttcgg
     2941 ccatttgtgc cttaaaatta ggatatcgtt tatcttgtaa tgtttctgac caatgttcga
     3001 taaatgcttt caatggtgct gaaatgctat accaatacac tggtgatgca aaaataattg
     3061 tatcactagc caatatttta tctagaatcg gcaaatagtc atcgtcatat gaagtaatag
     3121 tctctgctgt atgtctcacg tcacgtatcg gtttaaactg atgttgtgtc acgtcaatcc
     3181 attgatactc taaatcttgc aaagcgaatt ttgttaattg tgcagtatta ccgtttggtc
     3241 tactcccacc aaacaaaaca gtaatcattt tagcctaacc tcacttttga ttaataaata
     3301 tctgtgtttt tcgttaccta attatactat cataagcttt gcctaccgaa tagtaaaacg
     3361 cttacaactt ttatataaat ttgacgaaat ttcgtcatgc cttatataac gtcgtttgtg
     3421 atacggggct aattcatgat gaaattagat acatatatca ccattaaata caattcattt
     3481 agtcttcaat cggaaacagt tcatcgatat attgaatctc atcatctgat aaaacgatat
     3541 ctgcagcttt aatattttca acgacttgtt ctgcacgttt tgcaccagga ataatcacat
     3601 cgatagctgg tctcgttaaa taaaatgcta atacaatgtt cgcaattgaa gtttgatgtg
     3661 ctgcagctat gctttccaaa gcttttacgc gacgcacatt ttcttcaaat acacctggtt
     3721 taaaatcacg acgtgtagta cgatggtcac taaatttagt gttctcatca tattttccag
     3781 ctaaaatacc ggatgctaat gggaaatatg gaataaatgt gatttggtga tcaacacaat
     3841 attgtaatac tgcctcattt tcgcgatgca ataaattata ttctaactgt acaacatcaa
     3901 cgtaaccatc tttatttgct tctttaagtt gatctaatgt gaaatttgat acaccaattg
     3961 ctttaatctt cccttgttcc ttaagctctt gtaatgctgc aactgcttga tctttcggag
     4021 tgttgttatc cggaaaatga atataatata aatcgatata atcagtttgt agacgtttca
     4081 aactattctc aacttgttgt tttaaatatt ccggttgatt gttctgatgt acttcttgat
     4141 tttcatcaaa ttcatgagac cctttcgtag caattttaat ttgctctcgc ggatattctt
     4201 taacaacttc tccaaccaat tcttctgatc gttctggccc ataaatatat gccgtatcta
     4261 ataaattaat accatgatta atggcttgac gaacaacatc ttttccttgt tcttcatcta
     4321 agttcggata taaattatgc ccacctactg cgttcgtccc aagtgcgatt ggaaacactt
     4381 caacatcaga tttacctaag tttacaaatt gcttcattag acccagctcc ttatctttat
     4441 aacttttcca ctatcacact atcatgattt aatacaactt ccaaatgtta gaattagagc
     4501 ttgaatttaa gcggtgtaat aggaaaacgt tatataaaac tcagttaaaa taagtattgc
     4561 ttaaattgat atgcatatag cgcgattgct cgttgacctt taaattacat gtacttttga
     4621 atcagaaatt gaaaaacatt taagaacgac aatattaaag ccattgcaaa tttaatttgg
     4681 taatttctat tggtttttaa gtatgacatg aatgttttta tctataatta aagctatatt
     4741 aactagaa
//
LOCUS       DAMQYT010000187         4735 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMQYT010000187 DAMQYT010000000
VERSION     DAMQYT010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.191"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            22..261
                     /locus_tag="PGA33_002308"
     CDS             22..261
                     /locus_tag="PGA33_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDG6474101.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            382..2370
                     /gene="tkt"
                     /locus_tag="PGA33_002309"
     CDS             382..2370
                     /gene="tkt"
                     /locus_tag="PGA33_002309"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDG6474102.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            2648..2890
                     /locus_tag="PGA33_002310"
     CDS             2648..2890
                     /locus_tag="PGA33_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDG6474103.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            2877..2975
                     /locus_tag="PGA33_002311"
     CDS             2877..2975
                     /locus_tag="PGA33_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474104.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
     gene            3069..3536
                     /locus_tag="PGA33_002312"
     CDS             3069..3536
                     /locus_tag="PGA33_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HDG6474105.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            3660..>4735
                     /locus_tag="PGA33_002313"
     CDS             3660..>4735
                     /locus_tag="PGA33_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDG6474106.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIIISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNAIEISE
                     KDDMSIIEMFYKHITDKELSDIQS"
BASE COUNT         1727 a          633 c          890 g         1485 t
ORIGIN      
        1 aattgaaggt aggtttttta attgagtaat tctgatttga atatcgaaag aattaacgag
       61 ttagctaaaa agaaaaaaga agtaggatta actcaagaag aagcaaagga gcaaacagcc
      121 ttaagaaaag cttatcttga gagttttaga aaagggttta aacaacaaat tgaaaatact
      181 aaagtaattg atccagaagg taatgatgta acacctgaaa aaattaaaga gatacaacaa
      241 aaaagagata ataaaaatta aatcacaaat ctgtaaagaa ttttctgaca ttataacttg
      301 aaataagtat tttacttatc tttttatttt aaaataagtt ataatgtatt tgataaaatt
      361 gaagaaggga agatacacaa gatgtttaat gaaaaagatc aattagctgt tgatacgcta
      421 cgtgcactaa gtatcgacac aatcgaaaaa gcgaattctg gtcatccagg attacctatg
      481 ggagctgccc caatggctta cactttgtgg acacgtcatc tgaattttaa tccacaatct
      541 aaagattact tcaatagaga ccgtttcgta ttatctgcag ggcatggttc agcattattg
      601 tatagcttgt tacatgtttc tggtagttta gaattagaag aattaaagca atttagacaa
      661 tggggttcta aaacaccagg tcatcctgaa tacagacata cagatggtgt agaagttact
      721 accggaccac ttggacaagg ttttgctatg tcagtaggat tagctttagc agaagatcac
      781 ctagcaggga aatttaataa agaaggatat aatgttgtag atcattacac atatgtatta
      841 gcttctgacg gtgatttaat ggaaggtata tcgcatgaag cagcttcatt tgctggacat
      901 aataaattaa gtaaattagt tgttttatac gattcaaatg atatttcatt agatggcgaa
      961 ttaaacaaag ctttttctga aaacacaaaa gctcgttttg aagcatatgg ttggaattac
     1021 ttactagtta aagatggtaa tgatttagaa gaaattgata aagcgattac tacagctaaa
     1081 tctcaagaag gaccaacgat tattgaagtt aaaacaacaa tcggatttgg ttcaccgaat
     1141 aaagcaggaa ctaatggtgt tcatggggca cctttaggtg aagttgaaag aaaattaaca
     1201 ttcgaaaatt acggtttaga tcctgaaaaa cgttttaatg tttcagaaga ggtatacgaa
     1261 attttccaaa atactatgtt aaaacgtgct aatgaagatg aatctcaatg gaattcatta
     1321 ttagaaaaat atgcagaaac atatcctgaa ttagcagaag aatttaaatt agcgattagt
     1381 ggtaaattgc ctaaaaatta taaggatgaa ttaccacgtt ttgaactggg tcataatggt
     1441 gcatctcgtg ctgattctgg tactgttatt caagcaatca gtaaaactgt cccttcattc
     1501 tttggtggat cagcagacct tgctggttca aacaaatcca atgtaaatga tgcaactgat
     1561 tatagttctg aaacacctga aggtaaaaat gtgtggtttg gtgtacgtga atttgctatg
     1621 ggtgctgctg taaatggtat ggctgcacat ggaggtttac atccatatgg tgcaacattc
     1681 ttcgtattta gtgattattt aaaaccagcg ttacgtttat catcaattat gggattaaat
     1741 gcaacgttca tcttcacaca tgattcaatt gcagtaggtg aagatggtcc tactcatgaa
     1801 ccaattgagc aattagctgg attaagagcc attccaaata tgaatgttat ccgtcctgct
     1861 gatggtaatg aaacaagagt agcatgggaa gttgccttag aatctgaatc tacacctact
     1921 tcattagtat tgacacgtca aaacttaccg gtattagatg taccagaaga tgtagttgaa
     1981 gaaggcgttc gaaaaggtgc ctatacagtt tatggctctg aagagacacc agaattccta
     2041 ttattagctt caggttcaga agttagtctt gcagttgaag ctgctaaaga tcttgaaaaa
     2101 caaggtaaat cagtacgtgt tgtttcaatg cctaactgga atgcatttga acaacaatct
     2161 gaagaatata aagaatcagt tattccatca agcgtaacaa aacgtgttgc gattgaaatg
     2221 gcttcaccgc ttggatggca taaatatgta ggtactgcag gtaaagttat tgctattgac
     2281 ggctttggcg caagtgcacc tggcgattta gtagttgaaa aatatggatt tacaaaagaa
     2341 aatatcttaa accaagttat gagcttataa gaataattta taaagcgagt atgtttagaa
     2401 gtctaggatg cataatctta ggcttctttt aaagtgttga aatttagagt atagcactta
     2461 aactacatca taagtgataa gttatgaaag tatactattt cagattaatc tttaaaagct
     2521 ctgttataac agcatgattt ttgatattat ttttagtatc gatattaaaa tacttgaata
     2581 aactagttct tgaaataatg tgatgaattt agtaaaattc agtaggataa agaaagcggg
     2641 tgaaacaatg gcaacttggt tagcaattat ttttatagta gctgcattaa ttttaggttt
     2701 aattggaggt ttccttttag ctagaaaata tatgatggac tacttgaaga aaaacccacc
     2761 aatcaacgaa gaaatgcttc gtatgatgat gatgcaaatg ggtcaaaaac cttctcagaa
     2821 gaaaattaat caaatgatga cgatgatgaa taaaaatatg gatcaaaata tgaagagtgc
     2881 gaaaaagtaa attcgcaatt gatagaggct attttccaga tatggaaatg gcctcttttt
     2941 ataatcaaat taataagaat aaatatgttt attaaaatta aagttaacaa aatgacgaat
     3001 agactgagaa atgctataat tcattttgta tgatttacag agagtttatt taacgagaag
     3061 gtgtctgcgt gctctattta atattttcaa tcattgtagc tttatttatg ggaactatag
     3121 ttatagttat tcgtatgaaa gctcaaaatt atccggtaaa tgagaaaaaa atagttttgc
     3181 caccgttttt tatggcgacc ggtgcattga tgtacgtcgt tccatatttt aggctaacag
     3241 gatcggaaat gctagaagcc tttataattg gtttgctttt ttctacagtt ctaatttgga
     3301 cttctcgatt tgaagtcaaa ggtacagaaa tttatatgaa acgatctaaa gcatttccag
     3361 ttattttgat ttcattactt atcattcgta ctgtgatgaa aatattcatt agtaatgaaa
     3421 tagatcctgg agaattaggc ggcatgttct ttttattagc attctgtatg attgttcctt
     3481 ggagagcagc aatgctatat aaatacaaaa aactaaagaa aacattaatc aattaattac
     3541 ttttaaaacc acttgtgatc gacttctaaa tcagtcaatg agtggtttta attttacttg
     3601 aaaaggtgaa atgattatat taaaataaaa gcgaacaaat gttctatatg gaggcagtaa
     3661 tgaaaattat acatacagca gactggcact tagggaaaat attaaatggc aaacagcttt
     3721 tagaagatca agcgtatatt ttagatatgt tcgtagaaaa aatgaaagaa gaagaacctg
     3781 atatcattgt gatagctgga gatttatatg acacaacata tccaagtaaa gatgcaatca
     3841 tgttattaga acaagcgatt ggaaagttaa atttagaact gcgtatacca ataattatta
     3901 ttagtggaaa tcacgatggt aaagagaggt taaactatgg ggcgagttgg tttgaacata
     3961 atcagttatt tataagaaca gattttacat cgattaattc accaatagag ataaatgggg
     4021 ttaattttta tacactccct tatgctactg tgagcgaaat gaaacactac tttgaagatg
     4081 acaccattga aacacatcaa cagggaatta cgcgctgtat tgaaacaata gcaccggaaa
     4141 ttgatgaaga tgccgtcaat attttaatta gtcatctgac tgttcaaggt ggaaagacat
     4201 ctgattctga aagaccatta actattggaa cggttgaatc agttcagaaa ggtgtttttg
     4261 atatatttga ttatgtcatg ctaggtcact tgcatcatcc atttagtata gaagacgaca
     4321 aaattaaata tagtggctcc ttattgcagt attcattttc ggaagcgggt caagctaaag
     4381 ggtatagacg tgtaacaatt aatgatggca ttattaacga tgtatttatt cctcttaagc
     4441 cacttagaca attggaaatt atctcaggcg aatataatga tgttattaat gaaaaagttc
     4501 atgtgaaaaa taaagataat tatttacatt ttaaacttaa aaatatgtct catattactg
     4561 atccaatgat gagtttaaaa caaatttatc ctaatacttt agcgctgacg aatgaaactt
     4621 ttaattacaa tgaagaaaat aatgctatag aaataagtga aaaagatgac atgtcaatta
     4681 tcgaaatgtt ttataaacat ataactgata aagaattatc ggatatccaa tctaa
//
LOCUS       DAMQYT010000188         4704 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMQYT010000188 DAMQYT010000000
VERSION     DAMQYT010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.192"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..1238
                     /locus_tag="PGA33_002314"
     CDS             <1..1238
                     /locus_tag="PGA33_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDG6474107.1"
                     /translation="SGVVKIIEENRQQIQDTLNKDIVIKHILVRDKSKKRPLNISQYH
                     LTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVITANKDLLAVHLKLLED
                     LAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNGTSNFILSKMTKEQTT
                     FEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVIKLNDVKRRGISGVTL
                     TDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAAVEDEYNAIYVIGDAV
                     GDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFELKTDKTREMMDSDAEI
                     NIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDNQSYAAVIVGLESSPE
                     ELITKHGYEVDKVYPVEGV"
     gene            1244..2305
                     /locus_tag="PGA33_002315"
     CDS             1244..2305
                     /locus_tag="PGA33_002315"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDG6474108.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            2307..3221
                     /locus_tag="PGA33_002316"
     CDS             2307..3221
                     /locus_tag="PGA33_002316"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDG6474109.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            3279..4082
                     /locus_tag="PGA33_002317"
     CDS             3279..4082
                     /locus_tag="PGA33_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDG6474110.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYANEVTLDHNENGIPHALKKLL"
     gene            complement(4375..4689)
                     /locus_tag="PGA33_002318"
     CDS             complement(4375..4689)
                     /locus_tag="PGA33_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474111.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFMHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
BASE COUNT         1684 a          647 c          923 g         1450 t
ORIGIN      
        1 gatctggtgt tgttaaaatc atcgaagaga accgacagca aattcaagat acattaaata
       61 aagatattgt cataaagcat attcttgttc gagataaatc taaaaagaga ccgctaaata
      121 ttagccaata tcatttaact gaagatgtta atgaaatttt aaatgatgat tcattagata
      181 ttatcgttga agtcatggga ggaattgaac caactgtaga ttggttaaga acagcactta
      241 aaaataaaaa acatgttatt accgcaaata aagatttatt agcagtacat cttaaacttt
      301 tagaagattt agcagaagaa aatggtgtag ctttaaagtt tgaagcgagt gtagcaggtg
      361 gtattccgat cgtaaatgcc ataaataatg gtttgaatgc gaataatatt tcaaaattta
      421 tgggaatttt aaatggtacc tctaatttta ttttatctaa aatgactaaa gagcaaacga
      481 catttgagga agcacttgat gaagcgaaaa gacttggttt tgctgaagcg gatccaactg
      541 atgatgtaga aggggtagat gcagcgcgta aagttgtcat tacatcatat ttatcattta
      601 accaagtcat taaattaaac gacgttaaac gaagaggaat tagtggcgta actttaactg
      661 atattaatgt agccgatcaa ctggggtata aaattaaatt gattggtaag ggaatatatg
      721 aaaatggcaa agttaatgca tcggtagaac caacgttaat tgataaaaag catcaattag
      781 cagctgtaga ggatgaatat aacgcgattt atgttattgg tgatgccgtt ggtgacacga
      841 tgttttatgg aaaaggagca ggcagtttag caacaggtag tgccgttgtc agtgatttat
      901 tgaatgtagc attattcttt gaatcagatt tacacacatt gccaccacat tttgaattaa
      961 agacagataa aacacgggaa atgatggatt cagatgcaga aattaatatt aaagaaaaat
     1021 ccaatttctt tgtagtagtg aatcatgtca aaggttcaat tgaaaatttt gaaaatgagt
     1081 taaaggcaat attaccattt caccgatcat taagagttgc aaattacgat aatcaatcat
     1141 atgccgctgt tatagttgga ttggaatcat caccggaaga attaatcact aagcatggat
     1201 acgaagttga caaagtatac ccagtagaag gagtttaatt ataatgagaa gatggcaagg
     1261 attagtagaa gagtttaaag cacatttacc agtaaatgaa aatacaccaa aattaacatt
     1321 gaacgaggga aatacaccac tcattcattg tgaaaatatg tctaaaatac taggcataga
     1381 tttatatgtg aagtatgaag gtgccaatcc gacaggttca tttaaagatc gcggtatggt
     1441 aatggctgtg acaaaagcaa aagagcaagg taagaaaatt gtaatatgcg cttcgactgg
     1501 aaatacatca gcgtctgcag cagcatatgc agcgagagca ggtttaaaag ctatcgtcgt
     1561 aataccagaa ggtaaaattg cattaggtaa attgtcgcaa gcagtaatgt atggtgcaga
     1621 aatcgtttct attgaaggaa actttgatga agctttagaa attgtaaaag aaattgcaaa
     1681 aagtggcgaa atcgagcttg taaactctgt caatccattt agaatcgaag gacaaaagac
     1741 aggctcattt gaaattgtac aacaattaga cggtgaagca cctgatattt tagcgattcc
     1801 tgtaggtaat gcaggtaata ttactgcata ttggaaaggc tttaaagaat atcatgaagc
     1861 taaaggatca caattgccga aaatgtttgg cttccaagct gaaggcgcat caccaattgt
     1921 tcaaaataaa gtcattaaaa atcctgaaac gattgcaact gctattcgaa ttggtaatcc
     1981 tgctagttgg gataaggcga ctaatgctct taaagaatca aatggattaa tagatagtgt
     2041 tactgatgat gaaattctag aagcatatca gttaatgaca actaaagaag gtgtctttag
     2101 tgaaccagcg agtaatgctt ctattgcagg tttaattaaa ttgcatagac aaggtaaatt
     2161 acctcaaggt aaaaaagtag ttgctatttt aactggtaat ggattaaaag atcctgatac
     2221 tgctatttca ctactagata atccgataaa gccattgcca aatgataaag atagcattat
     2281 cgattatatt aaaggagctt tataacatgt cgaatgtttt ggagttaaca attcctgcat
     2341 caacagccaa ccttggagtt ggctttgatt ctataggtat ggctttagat aaatttttgc
     2401 atctgtctgt aaaggaaaca tcagggacaa aatgggaata tattttccat gatgatgcat
     2461 ctaagcaatt gcctactgac gaaacaaact ttatttatca tgtagcacaa caagttgctt
     2521 ctaaatatag tgttgacttg cctaatttat gtatcgaaat gagaagtgat attccattgg
     2581 caagagggtt aggttcgtca gcttctgctt tagtaggagc tatatatatc gcaaattatt
     2641 ttggtgatat ccaactgtct aaacatgagg tattacaatt agcgactgaa atcgaaggac
     2701 atcctgataa tgttgcgccg accatttatg gtggtttaat cgctggatat tataatgatg
     2761 tctcgaaaga aacgtcagtt gcacatatcg acataccaga cgtggatgtg attgtaacga
     2821 taccaactta tgaactaaaa acagaagcat caagacgtgc tttaccacaa aaattaacac
     2881 atagtgaagc ggttaaaagt agtgcaatta gtaatacaat gatttgtgca ttagcacagc
     2941 acaattatga attagcaggt aaactcatgc aacaagatgg ctttcatgaa ccgtatcgtc
     3001 agcatttaat tgctgaattt gatgaagtga aaacaattgc tagtcaacat aatgcctatg
     3061 caactgtaat tagtggtgct ggaccaacta ttttaatatt tagtcgtaaa gaaaatagtg
     3121 gggaattggt tcgctcttta aatagtcagg tagtatcatg ccattctgaa ttggtcgata
     3181 ttaatatcag tggtgttaaa gaacgaattg tataccaata gacgctttat attgtaaaat
     3241 agtattaaat gcagaataga gaggagattt aatgcgatat gacaaattat aaagttgtcg
     3301 ttttagatat ggatgacaca ttgctaaatt cagataatgt gatatcagaa gaaactgcaa
     3361 attatttaac agcaattcaa gatgaaggtt attatgttgt tctagcatct ggtagaccta
     3421 ctgaaggtat gattccaact gctagagatt taaaattacc tgaacatcat agctatatta
     3481 ttagttataa cggtagtaaa acgattaaca tgactaatga agaagtagaa gtaagtaaat
     3541 cgattggtaa gcaagatttc gatgaaattg tagattattg tcgagataga ggctttttcg
     3601 ttcttacata tcatgatggt caaattattt acgacagcga acatgagtat atgaatattg
     3661 aagcagaatt aacaggttta ccgatgaaac gtgttgatga tatcaaagcg tatattcaag
     3721 gcgatgtacc caaggtcatg ggtgtagatt atgtagcgaa tattacagaa gctagaattg
     3781 atttgaatgg tgtgttcaat gataatgtag atgctacgac aagtaagcca ttcttcttag
     3841 aatttatggc caaagacgtt tcaaaaggta atgcaattaa agcgttatgt cacaaattgg
     3901 gatattcggt ggatcaagtc attgcttttg gtgatagtat gaatgataaa tcaatgtttg
     3961 aagtcgcagg tctagctatt gctatgggga atgcatcaga tgaacttaag caatatgcaa
     4021 atgaagttac gttggatcat aatgaaaatg gtattccaca tgcgctcaaa aaattgttat
     4081 aaattttaaa ataagcctta acacatgata tttgaataag atatcttgtg gttaaggctt
     4141 tttatttttg tgaaaatgac ttcagttata ctatggagga tttgaaatac atattttaga
     4201 ttagtaatga tatcaaacga atagagtaaa tgtatatttt ttgaataaat caagtattaa
     4261 ctagtcacgg aaggtagata aataaatttg atcattctga ataataacga atgcattaaa
     4321 aaatgcagtg acaatctaac attaattgtt aaaatcactg caagcatctt gattttattt
     4381 agctgtttgt gtccagtcat gcttacctct gaaacgtttt acatgagcat atatttggtc
     4441 agcagtataa tctaaatttg tataaactgt aatactgaac gctgattggt tttcagcaaa
     4501 tgattcttcg gaaaataatt cctttaagtg cataaataaa tcattcattt gtttatggtt
     4561 taaaccggga tattccctta aagttctctt taataattgt ccgttctttt cttctaatgc
     4621 ttgaactaca ataacaaatc tttcatcttg ctttagtaca tcatcaaata aatttgtttg
     4681 tccaatcatt gaatttcacc cctg
//
LOCUS       DAMQYT010000189         4629 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMQYT010000189 DAMQYT010000000
VERSION     DAMQYT010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.193"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            528..2333
                     /gene="glmS"
                     /locus_tag="PGA33_002319"
     CDS             528..2333
                     /gene="glmS"
                     /locus_tag="PGA33_002319"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDG6474112.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            2565..3347
                     /locus_tag="PGA33_002320"
     CDS             2565..3347
                     /locus_tag="PGA33_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6474113.1"
                     /translation="MLIQLDQIGRMKQGKTILKKISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATSGTVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEIRNEAHQLLKLVGMFAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPTLAMIYVTHFIEEITANFSKILLLK
                     DGQSIQQGAVEDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            3415..4272
                     /locus_tag="PGA33_002321"
     CDS             3415..4272
                     /locus_tag="PGA33_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDG6474114.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAEHFLAE"
BASE COUNT         1647 a          633 c          900 g         1449 t
ORIGIN      
        1 aaaacaaaaa aattgtttta ggatttttaa aattttattg tgaaaatatt tgcaaaacaa
       61 aacaacaccg ttgtacaata atgattaatg gaaaggggga aagttcggca gtacagttaa
      121 agcgcctgtg caaataaata tttgtatttg aagattaaag gttaatatat gagtggcctt
      181 tatagagtgc aatatatgta tttgtagacg aggaggatag tgatcgaata gatcggcgga
      241 tgctatcccg gatgtggctc attcgttagc ttattaagta aaacattagg gtgacttaat
      301 ggacaaagtt aataagatcg ccagaaattg aatataaaaa atattaatat ggaaagtaca
      361 gtgtgagcaa tttgtatagt tgtaaaaata actatgctta atttgttatg gatgaatgcg
      421 atgatagcat gttcctattt atattatgaa agcagattgt caatctaaat tatcggcaat
      481 aaatcataat ttacgcgtac tattccaata tggaggaaaa tgtcgttatg tgtggaattg
      541 ttggttatat tggctatgat aatgccaaag aattattatt aaaaggttta gaaaaattag
      601 aatacagagg ttatgactct gcaggtatcg cagtagtaaa tgatgataat acaactgtat
      661 ttaaagaaaa aggtcgtatt gcagaattac gtaaagttgc tgatagtagc gattttgatg
      721 gacctgttgg aatcggtcac acacgttggg caacacacgg tgtaccgaat catgaaaact
      781 ctcatccaca tcaatcatca aatggccgtt ttactctagt tcataacggt gttattgaaa
      841 actatgaaga gttaaaaggt gaatacttac aaggtgtatc attcatttca gaaacagata
      901 cagaagttat cgttcaatta gttgaatact tttcaaatca aggactttca actgaagaag
      961 catttacaaa agttgtgtca ttattacatg gttcatatgc attaggttta ttagatgctg
     1021 aagacaaaga cacaatctat gttgctaaaa ataaatcacc attattatta ggtgttggtg
     1081 aaggtttcaa tgttatcgca tcagacgcac ttgcaatgtt acaagtgaca agcgaatata
     1141 aagaaatcca tgaccatgaa atcgttattg ttaaaaaaga tgaagttatt attaaagatg
     1201 cagatggaaa cgttgtagaa cgtgattcat atattgctga aattgatgca tcagatgctg
     1261 aaaaaggtgt ttatgcacac tacatgttaa aagaaattca tgaacaacca gcagtaatgc
     1321 gtcgtattat tcaagaatat caagatgcag aaggtaactt gaaaattgat caagacatca
     1381 tcaatgatgt taaagaagca gaccgcattt acgttattgc agcaggtaca agctaccatg
     1441 caggtttagt aggtaaagaa tttttagaaa aatgggctgg cgtaccaact gaagtacacg
     1501 ttgcatcaga gtttgtctac aacatgccat tattatctga aaaaccattg ttcgtttata
     1561 tttctcaatc aggtgaaact gcagatagcc gcgccgtatt agttgaaact aataaattag
     1621 gtcataaatc attaacaatc actaatgttg caggttcaac tttatcacgt gaagcagacc
     1681 acacattgtt attacacgcg ggtcctgaaa tcgcagttgc atctacaaaa gcatatactg
     1741 cacaaattgc agtattatca atcttgtctc aaatcgttgc aaaagagcat ggtcgtgaag
     1801 cagatattga tttattgaga gaattagcaa aagtaacaac agcaatagaa gcaattgttg
     1861 acgatgcacc aattatggaa caaattgcta cagatttctt agaaacaaca cgcaatgcat
     1921 tctttatcgg acgtactatt gactataacg taagtttaga aggtgcgtta aaacttaaag
     1981 aaatttctta cattcaagca gaaggttttg ctggtggaga acttaaacat ggtacaattg
     2041 ccttaatcga agaaggtaca ccagttgtag gtttagcaac acaagagaaa gttaatttat
     2101 caattcgtgg taacgttaaa gaggtagtag cacgtggtgc acatccatgt attatttcta
     2161 tggagggtct tgaaaaagaa ggcgacactt atgtcattcc tcatgtacat gaattgttaa
     2221 cgccattagt atcagtggtt gcattacaat taatttcata ctatgcagca ttacacagag
     2281 atttagatgt tgataaacca cgtaaccttg ctaaatcagt tactgtggaa taattcactt
     2341 ttttagaatc aatcatgtat taaaattaaa gtatatggca cccttttaga ttaatcgact
     2401 agaagggtgc ttttttaggt cgacttagct tttacttcat cttaatttgg cagaaatgcg
     2461 taaaaatgaa gtgttttatt tatttaaata gtctgacaat taagggtgtt atgttaatat
     2521 gattttatga gaagtatgga gtagcaataa aggggtgacc tcgcatgtta attcaattag
     2581 atcaaattgg gcgaatgaag caaggaaaaa caattttaaa aaagatttct tggcaaattg
     2641 ctaaaggtga taaatggata ttatatgggt tgaatggtgc tggcaagaca acacttctaa
     2701 atattttaaa tgcgtatgag cctgcaacat ctggaactgt taaccttttc ggtaaaatgc
     2761 caggcaaggt agggtattct gcagagactg tacgacaaca tataggtttt gtatctcata
     2821 gtttactgga aaagtttcaa gagggtgaaa gagtaatcga tgtggtgata agcggtgcct
     2881 ttaaatcaat tggtgtttat caagatattg atgatgagat acgtaatgaa gcacatcaat
     2941 tacttaaatt agttggaatg tttgctaaag cgcaacaata tattggttat ttatctaccg
     3001 gtgaaaaaca acgagtgatg attgcacgag ctttaatggg gcaaccccag gttttaattt
     3061 tagatgagcc agcagctggt ttagacttta ttgcacgaga atcgttgtta agtatacttg
     3121 actcattgtc agattcatat ccaacgcttg cgatgattta tgtgacgcac tttattgaag
     3181 aaataactgc taacttttcc aaaattttac tgctaaaaga tggccaaagt attcaacaag
     3241 gcgctgtaga agacatatta acttctgaaa acatgtcacg atttttccag aaaaatgtag
     3301 cagttcaaag atggaataat cgattttcta tggcaatgtt agagtaaata ttttgcaaat
     3361 aataagtaat aatgacaaaa tttaattaag ataaaatgga cagtggaggg caatatggat
     3421 aacgtaaaag caatattttt ggacatggat ggaacaattt tacatgaaaa taatcaagca
     3481 tcaacgtata cgaaagatgt cattaatcaa ttgagagaga aaggatataa agtatttttg
     3541 gcaacaggac gttcgcattc tgaaatacat caacttgtac ctcaagattt tgcggttaat
     3601 ggcatcatta gttcaaatgg aacaattgga gaagtagatg gagaaattat cttcaagcat
     3661 ggtttatcat tggctcaagt gcaacaaatt actaatttag ctaagcgcca acaaatttat
     3721 tatgaggtat ttccttttga aggtaataga gtttctttaa aagaagatga aacatggatg
     3781 cgagatatga ttcgtagtca agatcctatt aatggcgtaa gtcatagtga atggtcttca
     3841 agacaagatg cgcttgctgg taagatagat tgggtaacta agtttcctga aggtgaatat
     3901 tcaaaaattt atctattcag ttctaattta gaaaaaataa cagcatttag agatgaatta
     3961 aagcaaaatc atgtgcaact acagattagt gtttcaaatt catcaagatt taatgcggaa
     4021 acaatggctt atcaaactga taaaggtaca ggcattaaag aaatgattgc acattttggt
     4081 attcatcaag aagaaacgtt agttattgga gatagcgaca atgatagagc aatgtttgaa
     4141 tttggtcatt atacagttgc tatgaaaaat gcacgccctg aaatccaagc attaacttca
     4201 gatgtaacgg catacacgaa tgaagaggat ggcgcagcaa aatatttagc agagcatttt
     4261 ttagctgaat aataaaatag gtagttattt attatttaat ttacaatagt tgatgagtaa
     4321 tgtacaaaga gcagtaaagt tattttctat tagaaaatgt cttactgctc ttttgtatgc
     4381 ttataaatat ttgaatcatc tatatttaat tggacaaact ctatgagaat aaatattgtt
     4441 aaaactaata agataggaaa ttcattgatt ttgaataata tttcttgttt taaggtttaa
     4501 ctattgaatt gtatacttct ttttttagta gcaacagatc cgtctttaaa aaaattcaca
     4561 cctattgact gtttttgttc ttgattatat tctctatgaa gtgttaatcc actataaaat
     4621 caaccggtt
//
LOCUS       DAMQYT010000190         4572 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMQYT010000190 DAMQYT010000000
VERSION     DAMQYT010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.194"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            17..1258
                     /locus_tag="PGA33_002322"
     CDS             17..1258
                     /locus_tag="PGA33_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDG6474115.1"
                     /translation="MHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLSKLLVRATK
                     KWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGESLFNQGLDL
                     LQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHMFENEAYKY
                     LSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREKFALKPFDK
                     HQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVFNMMFGGDP
                     SSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISEFEKIKAGD
                     FTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDIQKVSREDI
                     VSVAEKAFLDTIYVLTKGGDK"
     gene            1258..2544
                     /locus_tag="PGA33_002323"
     CDS             1258..2544
                     /locus_tag="PGA33_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDG6474116.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFDTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            2544..3248
                     /locus_tag="PGA33_002324"
     CDS             2544..3248
                     /locus_tag="PGA33_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDG6474117.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            3353..4180
                     /locus_tag="PGA33_002325"
     CDS             3353..4180
                     /locus_tag="PGA33_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HDG6474118.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            4199..>4572
                     /locus_tag="PGA33_002326"
     CDS             4199..>4572
                     /locus_tag="PGA33_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDG6474119.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWI"
BASE COUNT         1710 a          600 c          843 g         1419 t
ORIGIN      
        1 acaatctcaa ccaaatatac atatcaaagt ttcaccaaca actaaattta aaacaactac
       61 tatagttttt aaatttatgg cacctttaga atatgacaca ataacagcta gatcattatt
      121 aagcaaatta ttagttcgag caactaagaa atggccaacc gataagtcgt ttaataatca
      181 cttagccgat ttatatggtg cgtatgtgaa tagtacaatt tcaaaattca aagatcagca
      241 tgtcattaca ttttcattag aaattgttaa tgaacgttat ttaagaaacg gtgaatcatt
      301 atttaatcaa ggattagatt tattacaaga aatcatttgg aatccattaa ttgaaaataa
      361 agcattcaat gataattttg ttaaccaaga gaaaacatta ttagccaaaa aaatagaagc
      421 aatggtagat aataaagcac aatattcgtt tttaaaatta ctcgaccata tgtttgaaaa
      481 tgaagcatat aaatacttat ctacaggaca actagaacaa atcccacata ttactgctga
      541 aacactatat catacatatc aatcaatgat taataatgat caatgttctg tttatgttgt
      601 cggcaatgta gaacctgaaa gtgttgagaa acaaatacgt gaaaaatttg cacttaaacc
      661 attcgataaa catcaattcc aacattctac tcatcattta cacgatgaag aagttgatta
      721 tattgttgaa tatgatgacg tggatcaagc taaattaaat atgggatacc gttttccaac
      781 acaatatgga caaagtggat atgctgcctt tgttgtattt aacatgatgt ttggaggaga
      841 tccttcatct gttttattta atgaagtgcg agaaaagcaa agtttagcgt actctataca
      901 ttcacaaatt gatggcaaaa atggctattt atttgttttg agtggggttt caagtgataa
      961 gtacgaaact gcaaaagaca ctattataag tgaatttgaa aaaataaaag caggagattt
     1021 cactgaagaa aaattagagt tagctaaaaa agtaatcatt tctcatcgat atgaatctga
     1081 agatcgtccg aaaagtatta tagagattat gcataaccaa atattattag agcaaccaca
     1141 aagcaaagaa acatttataa atgatataca gaaggtaagt cgcgaagata ttgtttctgt
     1201 tgctgaaaaa gcatttttag atacaatcta tgtgttgaca aaaggagggg ataaataatg
     1261 aaagagcgtt attatgaatt aatagacgaa agagtattcg aacaagaatt agaaaatggt
     1321 ttacgattat ttattatccc caaaccaggt tttcaaaaga catttgtcac ttacactaca
     1381 caatttggtt cattagataa tcaattcaaa ccccttggac aagaccaatt tgttactgta
     1441 cctgatggag ttgctcactt tttagaacat aaattatttg aaaaagaaga agaagactta
     1501 tttactgcgt ttgctgaaga taacgcacaa gcaaatgcgt ttacaagctt tgatcgtaca
     1561 agctacttgt tcagtgcaac tgataatatt gaaaacaaca ttaaacgttt acttacaatg
     1621 gttgaaacgc cttattttac aaaagaaact gttgataaag aaaaaggtat tattgcagaa
     1681 gaaataaaaa tgtatcaaga gcaacctgga tataaattaa tgtttgatac attgcgtgca
     1741 atgtatcaac aacatccaat acgtgttgat attgccggta gtgtagaaag tatatacgat
     1801 attacaaaag atgatttgta tctatgttat gaaacgtttt atcatccatc aaatatggtt
     1861 ttatttgttg ttggcgatgt ggatcctgaa gcaatatgtc gaatagtaaa acaacacgag
     1921 gatgctcgta ataaagttaa ccaacccaaa atcgaacgag gacttgttga tgaaccggag
     1981 gatgttaaag aagcatttgt tactgaatct atgaaaattc aatcaccaag actaatgctt
     2041 ggttttaaaa ataaaccatt acaagaagcg cctcaaaaat atgtacaacg tgatttagaa
     2101 atgtcattat tctttgagtt aatttttggg gaagaaacag atttttatca gaatttatta
     2161 aacgaaggac ttatcgatga tacatttggt tatcaatttg tactagagcc gacgtatagt
     2221 ttttcaatcg tgacaagtgc tactgaagaa ccagataaat taaaaaaatt attattagat
     2281 gagttgcgtg ataaaaaagg caatttccaa gatgcagaag catttgaact tttgaaaaag
     2341 caatttatag gtgaattcat atcaagttta aactcacctg aatatattgc taatcaatat
     2401 actaaattgt attttgaagg tgttagcgtg tttgacatgt tagacattgt tgaaaatatc
     2461 acgttagata gtattaacga aacgtcatcg ttatatttaa atttagatca gcaagtcgat
     2521 agtcgtttgg agattaaaaa gtaatgaaag cattagtatt aggtggttct ggttcaattg
     2581 gttctgagat agtcaaacaa ttattaactg atggatttga agtttatgtg caatattatc
     2641 gtactgatat aaatgaatta actagcaaat ttaatgatga taaagttcgt tttatacaag
     2701 cggatttatc tcaaacaatt gatattgaca aaacatttgg tgacattaaa tcattagact
     2761 gtttaatata tgcaagtggt cagtctttat atggtgtttt acaagatatg aaagaccatg
     2821 atattgatgc atgttatcag ttaaatgtct tgcaattaat tcgattatgt agatatttcg
     2881 ttgatgtttt acgtcaaagt gccaatggaa gaattattgt aatttcatca atttggggtg
     2941 agacaggagc tagtatggaa actatttatt cggcgatgaa aagtgcacaa ttaggtttcg
     3001 ttaaggcgct tagtcaagag cttgcactaa catcagtgac agtaaatgct atcgcacctg
     3061 gatttgtagc cggtaatatg gcaagtgagt ggcaagaaga tgaacttcaa gcaatgataa
     3121 ctgaattacc acaacagcga ttggttttac cgagtgaggt tgctcataca tgcgcctatt
     3181 tatatcaccc aaatgctaga agtgttactg gaactataca gaaagttaat ggtgcttggt
     3241 atatttaaaa tatagaacta tttttatgag caaatgtttt tatgattgat gttataatat
     3301 gaatattaca atgaatcata atttgttaat taaacaaggg gtgaggttca ccatgacagt
     3361 tgcagagaaa aaagaatggt acctagaata cgaaattgaa attaatagac cgggtctttt
     3421 aggtgatgta tctagtttat taggtatgtt aggtataagt attgttacaa ttaatggtgt
     3481 tgatcaaggt aaacgaggcc ttttaattaa aacagacaat cttgaaaaag ttgaacgttt
     3541 tgagcaaata gctcgtggta taaatgaaat tgaaataaca aagcttaaaa aaccagaatt
     3601 aagagaccgt cttgcagtaa gacatggaag atatattgag caagatgcaa aagataagaa
     3661 aacctttcga tttgagcgtg aagatttagg cttgttagta gactttttag ctgaattgtt
     3721 caaagaagaa ggtcataagt tgattggcat tagaggtatg ccacgggttg gtaaaactga
     3781 atcaattgtt gcgggaagtg tttgtgcaca taagagatgg ttatttatta gttctacttt
     3841 aataaaacaa actgtacgta gctctctaat taaaggggaa tatgatgcca atcatgtata
     3901 cattattgat ggtgcagtta ctgccagaga atctaatcca aaacatcaag agcttgttaa
     3961 cgaagttatg acgttaccat caatcaaagt cgttgaacat ccagatttat ttgttgaaac
     4021 aagtacttgt acaatggaag attttgacta tattatcgaa ttgagagaaa atgaaaatca
     4081 agaaatacat tacgaagaaa tgaagaaaca gacagtccaa agtaagaata atttagattt
     4141 tggagatccg tttggtggtg gttttggttt cttcgagtaa gtgtaaggag gctatgaatt
     4201 gaaaacggtc ggtgaagcgc taaaaggtag acgtgaaagg ttaggaatga ctttaacaga
     4261 attagagcaa cgtactggaa ttaaacgtga aatgctagtg catattgaaa ataatgaatt
     4321 cgatcaacta ccgaataaaa attacagcga aggatttatt agaaaatatg caagcgtagt
     4381 aaatattgaa cctaaccaat taattcaagc tcatcaagat gaaattccat cgaaccaagc
     4441 cgaatgggac gaagtaatta cagttttcaa taataataaa gacttagatt ataagagtaa
     4501 atcaaaagag ccaatacaat tattagtaat catgggtatt acagttttaa taactttatt
     4561 gttatggatc at
//
LOCUS       DAMQYT010000191         4544 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMQYT010000191 DAMQYT010000000
VERSION     DAMQYT010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.195"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(101..1033)
                     /gene="cysK"
                     /locus_tag="PGA33_002327"
     CDS             complement(101..1033)
                     /gene="cysK"
                     /locus_tag="PGA33_002327"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDG6474120.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            complement(1212..2093)
                     /gene="hslO"
                     /locus_tag="PGA33_002328"
     CDS             complement(1212..2093)
                     /gene="hslO"
                     /locus_tag="PGA33_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDG6474121.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            complement(2322..4415)
                     /gene="ftsH"
                     /locus_tag="PGA33_002329"
     CDS             complement(2322..4415)
                     /gene="ftsH"
                     /locus_tag="PGA33_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDG6474122.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHQQA
                     PNIEKPYDPNHPDNK"
BASE COUNT         1340 a          943 c          638 g         1623 t
ORIGIN      
        1 aaaatgttta atttaacgct catattttta aaaatataca tggtagaaaa aaagagcaag
       61 ttatctcaaa aagataactc actcttttaa atgacattaa ttagtcatcg aatgaatata
      121 aaggtgttga taagtagcgt tcaccattac tcggcaatac tgttactact gttttacctt
      181 ttcctaattc ttttgctttt tgaatggcag cataaatcgc agcacctgat gaaatacctg
      241 ctaaaatacc ttcctcttta gcaactcgac gagacatttc catcgctgta tcatttccta
      301 ctttaataat actgtcatag atttctgtat tcaaagtgcc tggaataaat ccagcaccta
      361 aaccttgtaa tttatgtgga cctggctcac caccgctcaa tactggagaa gcctcaggct
      421 ctatagcaac aatttcgatg ttaggatatt cttttttcag aactttacct acaccagata
      481 acgtaccacc agtaccaaca ccagctagga acgcatcgat agttttccct tcaaattgtt
      541 gtaataactc aggacctgta gttaactcat gaacttcagg gttcgcaggg ttttcaaatt
      601 gttgtggctc gaagtaacca tgttcttctt tcaattcttt agctttttta attgcacctt
      661 tcatcgcttc tgatccaggc gttaaaacta attccgcacc gtatgctttt aataaattac
      721 gacgctcttg gctcattgtt tcgggcatag taaatactgc tttatatcct ttagcagcac
      781 atacaaatgc taaaccgata cctgtattac cacttgttgg ttctacaatt gtatcgccag
      841 gtttaatttt gccttctcgc tctgcttttt caatcattgc taaagcaatt ctatccttta
      901 cagaaccacc tggattttga tattccaatt ttacataaac atctgctgca ttgtcatcta
      961 ctacatttct caatttgact accggtgtac cgccaataat ttgagtaata ttatctactg
     1021 gtttttgtgc cattaagaac actccttttt agttagttgt tgaaatctta gtttatttat
     1081 cggatataga attattttat caaataaatt ctagaaagca aacaaaatca ctaaggatta
     1141 tacaactatt tagttctaca tttcactaaa ccgctaaaaa taaacatttt agcgtattga
     1201 tttaaattaa attacgctaa actttctagc aacacgttta attcttcttc agtatattta
     1261 tatttatttc cacagaaatg acatactgct tcagcaccat gatcttcttt aatcatattt
     1321 tgaatctcag cctcgcccaa tcctttaata gcatttaaaa atttctcatg actacaatta
     1381 cattcaaatt gaacaggcat tttctctaaa atttgcacat ggtcttcacc taagatttcg
     1441 tttagtaatc cttctggcgt taatccttgt tcaattaatt tagaaactgg tgtcatttca
     1501 ctaattgctt tttctaattt tgaaattgtt tcatctttgg cacctggcat aacttgaatg
     1561 ataaatcctc ctgctgcttt aatcgtatta tcaggattta ccaatacacc aagacctacc
     1621 gatgaaggtg tttgttcact tgtagcataa taataagtaa aatcttcacc aagttctcct
     1681 gaaacaattg gacttgctcc agagaaatag tctttcattc caacgtcttt aacaaccata
     1741 atagatccat ttgtccctac cgctcgtctt acatcaagtt taccttgctc atttaatgga
     1801 aaatgagttt gtggatggtc tacataagca cgcacctcgc ctttagcatt tgcatcggca
     1861 ataattcgtc caataggtcc ttggccatct acagtgacag ttaatttttg atcacctttc
     1921 aacattgcgc ccatcatagc tgttgctgtc attgttcttc ccattgcagc agatgctgtc
     1981 ggccatgtat aatgtctcgt ttgtgcttct tgaacagttt cagttgtcaa agcagcataa
     2041 gccctaatct ctccatcaaa tgctaatgct ttaacaatat aatcgtgtgt cattatttca
     2101 atctcctcta ttactctata tttaaaaaat tactttactt cataaaatgc aacaattgta
     2161 cttattctac acccatcatt ctaaataatg aagtaacttg ttttacaatt attttctgct
     2221 ataacaattc aacgacttaa aatctaatac gtattttcaa aaacgataaa agtacctctt
     2281 tctataactt tatcatagaa agaggtactg aatataatcg attatttatt gtctgggtga
     2341 tttggatcgt aaggtttttc gatatttggg gcttgttgat gtgctggttc atctttttca
     2401 tcagatttat cagcttcttt tttatcctca gcaatatctt tttcttcttt acgatcttca
     2461 ctttcgtcac gttgtccatc ttctaattgc tctttacgaa tctcttcata agatttaccg
     2521 aatttaccat cattaaattc agaatcttca tctttaacaa ctttagctgc atcataatca
     2581 atttcaggta atttaccttc gtagaataat gattgaattt gttcagcaac taatgtttct
     2641 tctgttaata atgtttcagc aattaaaatt aattgttctt tgtgctctaa taaaatttgt
     2701 ttacaacgtt cgtattgttc tttaacgatt cgttgaactt ctttatcaat ttcatatgcg
     2761 atttggcttg aataattagg ctcaccttgc atatctttac ctaagaatac ttgaccattg
     2821 ctatgaccga actgtaatgg tcctaatttt ttactcatac catattgcgt aaccattgag
     2881 cgtgcgattt gtgttgcacg ttcgaagtca tttgaagcac ctgttgatac ttcgttaaag
     2941 ttaatatctt ctgatacacg tccaccaagt aaaccacaga ttttatctaa taactcttgt
     3001 tcagtcatta agaaacgatc ttgtttaggt agcatcattg cataaccacc tgcttgtcca
     3061 cgtggaacaa tagtaacttt atgcactact tctgcctcat caagtaccat accgataatt
     3121 gtatgaccag cttcgtgatg agcaacaata ttacgttctt tcttagaaat aactcgagat
     3181 ttcttagcag gtccggctat aactctatcc gttgcctctt cgatatctct catgtcaatt
     3241 ttctttttac cttcacgtac agcaattaaa gatgcttcat ttaataagtt ctctaaatca
     3301 gcacctgaga aaccaggtgt acgttgtgaa attgctttta aatcaaccgt ttcatcaagt
     3361 ggtttgtttt tagcatgaac atgaagaatt gcttcacggc ctttcacatc tggacgacca
     3421 acttgaattt gtctatcaaa acgacctgga cgtaataagg ctgggtcaag gatatcagga
     3481 cggtttgtag cagctatcat aatgatacct tcattttcac cgaaaccatc catttcaact
     3541 aataattggt ttagggtttg ttcacgttca tcatgaccgc caccaacacc tgcaccacgt
     3601 tgacgaccaa cagcatcaat ctcatcgata aagatgatac aaggcgcgtt tttcttagca
     3661 ttatcgaata agtcacgaac acggctcgca ccaacaccaa caaacatctc tacaaagtct
     3721 gaaccactaa tagagaagaa tggtgcgcca gcttcacctg caaccgctct agcaagtaat
     3781 gttttaccag tacctggagg tccaacaagt aagacacctt taggaatcct agatcccatt
     3841 tctttgaatt ttttattatc tttcaagaaa tcaacaattt caattaattc ttgtttttct
     3901 tcatctgccc ctgctacatc agagaaacga acacgacgtt tattattatc gtacattttt
     3961 gctttagatt taccaaagtt catcatacga ccgccactac cgccaccttg tgcttggctt
     4021 aggaagaaaa taaataataa cgctatgact acaactggaa ttaatgttga aagtatactc
     4081 acaaagacac tttgtttttc ttcttcttta atcgttaatt ttacaccgtt ttgcttttta
     4141 gcagcatcag taattttttg taattctttt tcgttgttat ataaaatagt tgatgaatag
     4201 tcttcatcat ttttcgtttt accacttacc atatagacat tttgttgtgg ttggatttct
     4261 aaagttttaa ggtcaccttt ttccaacttc tcagtaaatt gattatatgt aagctgtttc
     4321 ggcatatttc cattaccgtt taaatatgaa aatagaccaa aaataataac gcctattatt
     4381 acgataacta gcacattgcg aaaagctttc tgcatgcgtc atttcctcct acttcccata
     4441 ataaaactaa caaaaattgt aacatatgac atgttttata agctagtaat tgagcttaac
     4501 atcaatgtca ttcacacccg tattagtttt atcaatctct atat
//
LOCUS       DAMQYT010000192         4505 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMQYT010000192 DAMQYT010000000
VERSION     DAMQYT010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.196"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(52..687)
                     /locus_tag="PGA33_002330"
     CDS             complement(52..687)
                     /locus_tag="PGA33_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HDG6474123.1"
                     /translation="MLGTDELYKVLYEHLGPQFWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIHSSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            complement(1075..2736)
                     /locus_tag="PGA33_002331"
     CDS             complement(1075..2736)
                     /locus_tag="PGA33_002331"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDG6474124.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(2733..3149)
                     /locus_tag="PGA33_002332"
     CDS             complement(2733..3149)
                     /locus_tag="PGA33_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDG6474125.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(3242..3349)
                     /locus_tag="PGA33_002333"
     CDS             complement(3242..3349)
                     /locus_tag="PGA33_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474126.1"
                     /translation="MKDSENLVFFGFEVLFVFYKMAYIYEALIKYGIVN"
     gene            complement(3424..4434)
                     /gene="adhP"
                     /locus_tag="PGA33_002334"
     CDS             complement(3424..4434)
                     /gene="adhP"
                     /locus_tag="PGA33_002334"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDG6474127.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
BASE COUNT         1387 a          909 c          578 g         1631 t
ORIGIN      
        1 taaattattt tttatatttc atataaactc atctatctaa cttcacagcg tttacttttt
       61 aaagtaaggt tctaaaaaat cataattctt tatatctttg tctctaaagt aatgtttacc
      121 aaatacatct aacagagcat gaaattcatt agcatcttga tttgtaaaat gatttggtaa
      181 tgtgactact ttttttaatt gatcataatt tttagtgttt tcatatccta atttatcata
      241 tatttttctt gtatagctat ctggaataaa ttcaatacgt ccgaatatat aaacaagtaa
      301 gacatctgct gtttcacttc caataccttt caaagataat aattcttttc ttaatccacc
      361 tttatatcgc tcattaatct cttgataatt gaaatgatgt cgtgctaacc atgttaataa
      421 tgttttaatc gtcagtgatt tacttttata aaagccactt gaatgtatca atgattgtaa
      481 cgtttcaata ggtagttcta atatatgatt tggattaaaa tgcgtatgtt ctttaatctg
      541 attcaatgca atttctgcat ttcgccatct agtattttga actaaaattg cacctaacat
      601 catttcaatg tcattatcag caggccacca aaattgtggt ccgagatgtt catataaaac
      661 tttatataat tcatcagttc ctaacattta caccctccat tttagaggga gtgggacaga
      721 aatgatatta tcgtaaaatt tatttcgtcg tcccacccca acttgcattg tctgtagaaa
      781 ttgggaatcc aatttctctt tgttggggcc ccgccggcaa ggttgactag aattgaaaaa
      841 agcttgttac aagcgaattt tcgttcagtc aactactgcc attataactt tgtagagcat
      901 agaacattga tttatgtctc aggctctctt atgcagttgt gcagagcatc caactgtgtt
      961 cctcttaacc ttattcacat actaagcatt ccccaatatg tcacatacct aaaaaaacac
     1021 tccaacacaa agtatctttc gagaaactct acgttggagt gtatataaat gttcttacat
     1081 tgattcaggt gcgcttacac cgaccattgc caatgcattt ttcaatgtaa ttctgaccgc
     1141 ttcaatcata gcaacatgtg cttttgtttt ttcaatatca tctgttaaca ctttttcagc
     1201 attatagaat ttatggaaat gagaagctaa atcttgaata taattagtaa ttctatgtgc
     1261 cgatctatgc tcagcagcac tttcaattgt aggttcgaaa tcagctactt ttttcaacaa
     1321 ttcaatcgct ttttcattag taatcgttgt aaaatcattc gcagcagtca cttcaatacc
     1381 ttgctctttc gcttgtttta aaattgaaca aatacgcgca tgtgcatatt gagcatagta
     1441 aactggattg tcttgagatt gctctttcgc taattccata tcaaaatcaa agtgactatc
     1501 aggactacgc atagttaaga aataacgtgc agcgtcaacg ccaacttcgt ccataatttc
     1561 tcttaatgta atcgcattac cagtacgttt actcatcttc acttctttac cattttccat
     1621 taaacgaacc atttgcatga tttgaatttc taaacgatta ctatctacac caaacgtttc
     1681 aagagatgct ttcaaacgat taatataacc atgatgatca gcaccaaata aatcgattaa
     1741 aatgtcatta ccacgtttta ctttatcgaa gtggtacgca atatctggta agaaatacgt
     1801 atatgtaccg tcatttttaa ttaatactct gtctttgtcg tctttaaaat cagttgtacg
     1861 taaccatgta gcgccatcag cttcatacgt ataacctaat tctttcattt ttgctaaaac
     1921 ttcaagaatt tctccttttt catataaaga tgtttcacta aaccaattat caaaatgcgt
     1981 attgaactct gctaaatcat ttttcaattt agccatttcg tattctacgc ctaattttct
     2041 aaattctttc aaacgtgctt cttcagaata atctttaatt tcagggtgtt tctctgctaa
     2101 atctttacct atttcaataa tatcttttcc attatagcca tcttctggca ttgaataact
     2161 attgtcacct aaagcttcaa agaaacgtgt ttcaatcgaa cgcgctaagt tagtaatttg
     2221 attaccagca tcattaatat aatattcacg tgttacatta tagccagctg cagttaaaat
     2281 attagctaaa gcatcaccaa ctgctgcatt tctagcatga ccaatatgta aatctcctgt
     2341 agggttagct gaaacatact caagcaatac attttgacct tttgattcat ttacatgtcc
     2401 aaattgatca cctttttcaa ttgcttcagg aataattgct gttaaatact gattatctaa
     2461 gtaaaaatta atgaatcctg gaccagcaat gtcaatttgt tttacatgtg ctttttcagt
     2521 atctaagtta tcaacaatcg cttgagcaat ttcacgagga ttacgctttg caatcttagt
     2581 cagtaccatc gcaatattag tagcataatc tccatttttt gtatctttag gaacttcaat
     2641 tttaatatca ggaatctcat ctgctaatcc tgctttgtta atacttgctg caatttcttc
     2701 tactaatgtt tgtttcactt gatcaataat attcattcgc ctatctcctt atagttaatt
     2761 tcatattgat aagaacccat tttttcatta tcttgatata attcataatg aatctttagc
     2821 ttgccaccgt ctccacttac gaaatgtaaa atgcgtaatg ttttaacttc tagtggaatt
     2881 cgtccagccg atatatcgta aaaagttgtt gtcgtttgtc cttcaacaaa atgcaaattc
     2941 atattaatgt cgcctttacg aatcaactta acgctatcat catccacttt tattgtaaca
     3001 ttaactgttg catcctcaat ttgttctaca taccgaataa agttagattg cctttgttgc
     3061 caagttcctt cagtagtaaa ttcaaatttt tctttttcgt tgtgctgttt caacacttgc
     3121 tttgtttgaa tacttacttt tttatccaat ttactactca ccttaatctt cgcataatag
     3181 aaatattata gcataaaata aggtcatctt gttatgcctt cttaaagcta aataggttca
     3241 attaattaac aattccatac ttaatcaacg cttcatatat gtaagccatt ttataaaata
     3301 caaacaacac ctcaaaacca aaaaacacga gattttcact atctttcatg aaaacctcgt
     3361 gtttgattat aatgatagtg ttcagagcac taccttcttt atctatatag tcagttgata
     3421 tttttaaaat ttaataacca ttctaccagt tatagtacca ttttccattt cttcaaaaat
     3481 atcattgatt tcttctaatt ttcttaattg aactttaggt gttactttat tttcagcagc
     3541 aaattcaaac gcttcacgta agtcttgtct tgtaccaaca agtgaaccta ctacttcaat
     3601 accatcaagc actaatcttg ggatatctaa gttcatttta tcaacaggta aaccaacggc
     3661 aacaactctt gcaccagctt ttacaacatc aaccgcttgg ttaaatggcg tcttagcaac
     3721 tgaagttacc actgttgcat ctaatccttt gttatcagtt aatttcataa cttctgcaac
     3781 tggatcaaca tctttagaat taataatagc atcagcacct aattctttcg cgaatgctaa
     3841 tttatcatca ttgatgtcga aggcaacaat tttagccccc ataacgtttt tagcatattg
     3901 taaagctagg ttacctaaac cacctatacc aaaaacacct aaccattgtc caggttttac
     3961 attacttact tttacagctt tataagttgt cacacctgcg catgtaatag aagacgctgc
     4021 tgctggatct aatttttcag gtactttcac agcatagtct gcagtaacaa taacttgttc
     4081 agccattgca ccatctactg tataaccagc atttttcaca ctacggcaaa gtgtttcacg
     4141 acctgttgta caatattcac atcttccaca gctttcgaac atccaagcga tagacacacg
     4201 gtctccaatt tttaatgatt ctacatcttc cgcaacttcg atgactttac caataccttc
     4261 atgacctaaa gtaacgcctg taacatcacc aaaatcagca ttcttaacat gtaaatcggt
     4321 atgacaaacg ccacaatatt ccgtttgtac caacgcttca ccaggtttta aagctcttaa
     4381 ctttttgtcc tcaatactta ctttgtgatc tttcgttaca actgctgctc tcataataat
     4441 gtcctcctta tagtcactcc agtatgtatg atattaattg gctattactt tcaaaaaaga
     4501 aaacg
//
LOCUS       DAMQYT010000193         4490 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMQYT010000193 DAMQYT010000000
VERSION     DAMQYT010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.197"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            96..233
                     /locus_tag="PGA33_002335"
     CDS             96..233
                     /locus_tag="PGA33_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474128.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(497..1699)
                     /locus_tag="PGA33_002336"
     CDS             complement(497..1699)
                     /locus_tag="PGA33_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDG6474129.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            2017..3087
                     /locus_tag="PGA33_002337"
     CDS             2017..3087
                     /locus_tag="PGA33_002337"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDG6474130.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            3102..4460
                     /locus_tag="PGA33_002338"
     CDS             3102..4460
                     /locus_tag="PGA33_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDG6474131.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLIYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLINDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
BASE COUNT         1680 a          638 c          808 g         1364 t
ORIGIN      
        1 tttgaaaata aattaaaaat tttgtaaatg atgtagtaaa ggtatgtcga atagggtaag
       61 ataatattga aagcgatcca aacgaggtga taataatgaa acgtcctgaa aagattcaaa
      121 atgtagtcaa actattgtca tcattaggtg tgaatattaa aaaaactaaa tctcgtttag
      181 acattattaa tactttgcca gcatctaata aagtaagtca cgaattaaaa taattgactg
      241 tgattgtgaa tcacacaccg ttaaagctat cgtcggacta ttcgatgata gcttttttga
      301 ctggatttac taaaatgaaa gcaagtacta tataacaaaa tccctcttta gagtgtacat
      361 tttacaatgt gtacttcaaa gagggattgt tattatcgag taatatctat atatgacatt
      421 ttaaatcgac gacgtaaata gactatataa cgtcatcttg aaaagttatg atatttgaaa
      481 ctcaaaatag tttaaattaa ttactttttg gatggtataa atcgacatat cgttttggtt
      541 catgataata aattgataag actataccta ttccagtcat catactccat agcgcactac
      601 caccataact aataaatggt aatggaatac cagtgattgg taacaactga attgtcatac
      661 caatattttg taaaatatgg aacacaagta aagtgacgaa accaacgata aagattttgt
      721 taaattgatc ttcaattttc gcagctaatc ttattagatg gaagattaaa aataaaaata
      781 ttaagatcaa tatgacagaa ccgataaagc caagttcctc tccaatcact gaaaagataa
      841 agtcagtatg attttcaggt atataaactt caccgtgatt gtatccttta cctagtaact
      901 gtccagaacc gatagcttta agtgattcag ttaaatgata gccatcacca ctactatatg
      961 tataggggtc aagccatgaa ttgattcgtc ccatttgata cagttggaca cctaataaat
     1021 tttcaattaa tgcgggtgca tatagaatac ctaaaatgac tgtcattgca ccaacaatac
     1081 ctgtaataaa gataggtgct aagatacgcc atgttatacc acttactaac atcacacctg
     1141 caataatagc agctaatact aatgtagttc ctaggtcatt ttgcagtaat attaaaatac
     1201 ttggtactaa cgagacacca ataattttga aaaataataa caaatcactt tggaatgatt
     1261 tattgaatgt gaattgatta tgtctagaaa cgacacgcgc taatgctaaa attaaaataa
     1321 ttttcatgaa ttcagatggc tgaatactga tagggccaaa cgtgtaccaa cttttggcac
     1381 cattgataat aggtgtaata ggtgactcag gaataacgag caagcctatt aataatagac
     1441 agattaagaa atacaataaa tatgtataat gtttaatctt tttaggtgaa ataaacatga
     1501 tgatacctgc aaaaattgca cctaaaatgt aataaaaaat ttgtctgata ccgaaattag
     1561 cactgtattg accaccgccc attgccgagt taataagcag aacactgaaa attgctaaaa
     1621 cagctatagt ggctactaat acccagtcta ctttgcgaag ccaatgctta tccggctgtt
     1681 gacgagatga ataattcatt gcaaactcct tttatactca ctaatgttta tatcaatttt
     1741 acatgacttt ttaaaaatta gctagaatat cacagtgata tcagctatag atttcaattt
     1801 gaattaggaa taaaatagaa gggaatattg ttctgattat aaatgaatca acatagatac
     1861 agacacataa gtcctcgttt ttaaaatgca aaatagcatt aaaatgtgat actattaaga
     1921 ttcaaagatg cgaataaatc aattaacaat aggactaaat caatattaat ttatattaag
     1981 gtagcaaacc ctgatatatc attggaggaa aacgaaatga caaaagaaaa tatttgtatc
     2041 gtttttggag ggaaaagtgc agaacacgaa gtatcgattc tgacagcaca aaatgtatta
     2101 aatgcaatag ataaagacaa atatcatgtt gatatcattt atattaccaa tgatggtgat
     2161 tggagaaagc aaaataatat tacagctgaa attaaatcta ctgatgagct tcatttagaa
     2221 aatggagagg cgcttgagat ttcacagcta ttgaaagaaa gtagttcagg acaaccatac
     2281 gatgcagtat tcccattatt acatggtcct aatggtgaag atggcacgat tcaagggctt
     2341 tttgaagttt tggatgtacc atatgtagga aatggtgtat tgtcagctgc aagttctatg
     2401 gacaaacttg taatgaaaca attatttgaa catcgagggt taccacagtt accttatatt
     2461 agtttcttac gttctgaata tgaaaaatat gaacataaca ttttaaaatt agtaaatgat
     2521 aaattaaatt acccagtctt tgttaaacct gctaacttag ggtcaagtgt aggtatcagt
     2581 aaatgtaata atgaagcgga acttaaagaa ggtattaaag aagcattcca atttgaccgt
     2641 aagcttgtta tagaacaagg cgttaacgca cgtgaaattg aagtagcagt tttaggaaat
     2701 gactatcctg aagcgacatg gccaggtgaa gtcgtaaaag atgtcgcgtt ttacgattac
     2761 aaatcaaaat ataaagatgg taaggttcaa ttacaaattc cagctgactt agacgaagat
     2821 gttcaattaa cgcttagaaa tatggcatta gaggcattca aagcgacaga ttgttctggt
     2881 ttagtccgtg ctgatttctt tgtaacagaa gacaaccaaa tatatattaa tgaaacaaat
     2941 gcaatgcctg gatttacggc tttcagtatg tatccaaagt tatgggaaaa tatgggctta
     3001 tcttatccag aattgattac aaaacttatc gagcttgcta aagaacgtca ccaggataaa
     3061 cagaaaaata aatacaaaat tgactaactg aggttgttat tatgattaat gttacattaa
     3121 agcaaattca atcatggatt ccttgtgaaa ttgaagatca atttttaaat caagagataa
     3181 atggagtcac aattgattca cgagcaattt ctaaaaatat gttatttata ccatttaaag
     3241 gtgaaaatgt tgacggtcat cgctttgtct ctaaagcatt acaagatggt gctggggctg
     3301 ctttttatca aagagggaca cctatagatg aaaatgtaag cgggcctatt atatgggttg
     3361 aagacacatt aacggcatta caacaattgg cacaagctta cttgagacat gtaaacccta
     3421 aagtaattgc cgtcacaggg tctaatggta aaacaacgac taaagatatg attgaaagtg
     3481 tattgcatac cgaatttaaa gttaagaaaa cgcaaggtaa ttacaataat gaaattggtt
     3541 tacctttaac tattttggaa ttagataatg atactgaaat atcaatattg gagatgggga
     3601 tgtcaggttt ccatgaaatt gaatttctgt caaacctcgc tcaaccagat attgcagtta
     3661 taactaatat tggtgagtca catatgcaag atttaggttc gcgcgagggg attgctaaag
     3721 ctaaatctga aattacaata ggtctaaaag ataatggtac gtttatatat gatggcgatg
     3781 aaccattatt gaaaccacat gttaaagaag ttgaaaatgc aaaatgtatt agtattggtg
     3841 ttgctactga taatgcatta gtttgttctg ttgatgatag agatactaca ggtatttcat
     3901 ttacgattaa taataaagaa cattacgatc tgccaatatt aggaaagcat aatatgaaaa
     3961 atgcgacgat tgccattgcg gttggtcatg aattaggttt gatatataac acaatctatc
     4021 aaaatttaaa aaatgtcagc ttaactggta tgcgtatgga acaacataca ttagaaaatg
     4081 atattactgt gataaatgat gcctataatg caagtcctac aagtatgaga gcagctattg
     4141 atacactgag tactttgaca gggcgtcgca ttctaatttt aggagatgtt ttagaattag
     4201 gtgaaaatag caaagaaatg catatcggtg taggtaatta tttagaagaa aagcatatag
     4261 atgtgttgta tacgtttggt aatgaagcga agtatattta tgattcgggc cagcaacatg
     4321 tcgaaaaagc acaacacttc aattctaaag acgatatgat agaagtttta ataaacgatt
     4381 taaaagcgca tgaccgtgta ttagttaaag gatcacgtgg tatgaaatta gaagaagtgg
     4441 taaatgcttt aatttcatag agattagtcg agggaccttt tacttataaa
//
LOCUS       DAMQYT010000194         4452 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMQYT010000194 DAMQYT010000000
VERSION     DAMQYT010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.198"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..338)
                     /gene="deoC"
                     /locus_tag="PGA33_002339"
     CDS             complement(<1..338)
                     /gene="deoC"
                     /locus_tag="PGA33_002339"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDG6474132.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVV"
     gene            complement(419..1771)
                     /gene="tet(38)"
                     /locus_tag="PGA33_002340"
     CDS             complement(419..1771)
                     /gene="tet(38)"
                     /locus_tag="PGA33_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDG6474133.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
     gene            complement(1778..2485)
                     /gene="deoD"
                     /locus_tag="PGA33_002341"
     CDS             complement(1778..2485)
                     /gene="deoD"
                     /locus_tag="PGA33_002341"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDG6474134.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            2721..3476
                     /locus_tag="PGA33_002342"
     CDS             2721..3476
                     /locus_tag="PGA33_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDG6474135.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            complement(3668..4393)
                     /gene="sasD"
                     /locus_tag="PGA33_002343"
     CDS             complement(3668..4393)
                     /gene="sasD"
                     /locus_tag="PGA33_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HDG6474136.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALRQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
BASE COUNT         1466 a          809 c          698 g         1479 t
ORIGIN      
        1 accactgctt caatgtcttg ttgtacatca tcaaaacgtc catcttttaa tgcgccgatg
       61 ttgatgacca tgtcaatttc atctgcacca ttttgaattg catcttctgt ttcaaatgct
      121 ttcgttgcag ttgtcgacgc acctaatggg aatcctatta ccgtacaaac gagcacctct
      181 gaatcagcta gtcgctctgc tgcatattta acatgtgttg gattcacaca tacagattta
      241 aaattgtatg ctttcgcttc atcgatgatt tgatcgattt gcgtacgtgt tgactcaggc
      301 ttcaataaag tgtgatctat atatttctca aatttcatac ttactactcc tcgtgttata
      361 taatctcttt atttaatttt actataaata cgaatatatc tcgcgaattt atatctattt
      421 atttttcaga ttgtgtccaa cgatttaagc tgacgaataa aactaatccg acaatactca
      481 ttaaacctaa aataattaaa ataataccga aatgtgaact taatgcatca ttgcctggga
      541 aatttaatgc tttaaaatcg attagagccg cagcaatcgc aatacctaca gataccgcca
      601 cattaataat taaattataa aaaccaatag ccacacctgt catattaaga tctattgttt
      661 taatggcttc gttaagtaaa ggtgcataca ttaaagcaaa gctacctgca aagaatatca
      721 tagaaatgac gaagattgaa atgtgattac ctactgcaaa tgcaggtaaa atcaagctca
      781 gtgctattaa aataattgct gtgataatcg cttgttttga attcagatat tcgccgattt
      841 taccacttag tgcaccaaca atgactgcta ctatataacc cggtactaat aacagtgatg
      901 ttgtgtctag ttgcagatga taaatttgct ccattatgaa tgggaacgta aaaatataac
      961 ccaattggat agcatacatt acaaatacta taaataaaaa tgaagcataa cgtttatttt
     1021 ggaaaaatga tttatttact aatggacgtt gcgcattttt aatatatagc gcaaaaacga
     1081 taatcgcaat taaggcacca atcatatata accaattaaa gttcgtaata aacagcatga
     1141 ctgttgtagc aatagttgcc actagaatta agccaacaaa atctaaatga gctttattcg
     1201 tattattttc ttttggtaat aatttaaata ggaatggcaa cgtaaatact acgattaatg
     1261 caattaaaaa catatttgtc cagtgtaaat acgtagaaat aaatccacct gataatgtac
     1321 cgataactaa tgacaaggaa taactgctcg tacttaagcc aaggtaagtc ttctggtcct
     1381 ctttagaaag atactttgca acatatatca catataatgt ctctgcagca gctaaaccgg
     1441 cagtttgaat aatacgtcca actaaaagta atgggaattg atgttggaaa atgtaaccaa
     1501 taattgatcc gataatgatt aatatcacgc cataaataaa tagtgtgcgt atactaatgg
     1561 catcagataa tgaagcgtat actacagcgc caataccaat tactaaaccg gctaatgtcg
     1621 cttgccaact tactgtcgtt acagatatgt ttaagtcatc agcaatggct acagaaatca
     1681 atttaaatga gttgtctata accaaactga atacaaataa gaataataaa ataggtactg
     1741 ctttctttat tttagaatat tcaacattca taatcatcta caccaatgac agtgcaattt
     1801 caatcatatc tgtaaatgca cgttcccttt cctcaggtgt tgttgacgtt tcatgaatta
     1861 aatgatcgct cactgtgaac acacctaatg cttcgacacc agcgtaaatt gcattcatgt
     1921 ataatgcagc tgattccatt tctacaccta aaatacccat acgcatccaa cgttcactcg
     1981 ctgttgtatc cgcgttatag aaaatatcac ttgataacac attacctaca tgatgacgta
     2041 cacctttgtc acgtgctgtt tcaactgctt tttctaataa ttgataagaa gcaattggcg
     2101 caaaatgacc tggtaattga tattgttgaa cgtaatttga atcagtagag gcaccttgtg
     2161 caataatcac atcatataaa tcaatgtttt cttgcatcgc gccacaagag ccaacgcgaa
     2221 ttaatttttt acaaccaaat gtatgaatta attcataaga gtaaatgcca atagatggca
     2281 tacccatacc tgaacccatg acagaaactt ttttaccttt atatgttccg gtaaaaccaa
     2341 acatgtttcg cactgtattg aactgttcca catcatccaa ataagtttct gcaatgaact
     2401 tagctcttaa cggatctcct ggcaatagaa ccgtttctgc aatttcgacg tcattcattg
     2461 gtttaatgtg tggtgttgat ttcataaaat attcctccca atatttgtat agtgatttgt
     2521 ctatttgtct aaccatgtat ataataatat tttttaaaag cgctttcaag cattacattt
     2581 tgacaatatt aagaacattt tacagttaca gctactaacc ttgtataaaa ctaattttca
     2641 aagcctatct atatttaatt tttataattt ttccaatttg agtaaaaatt attgtatgat
     2701 gagagtgatt aagtaacttt atggaggttg tctacgtgac gcaaacacaa ccaaaatttt
     2761 taacgattta taatacgtta tataaagaaa ttcaaatagg aaaatatccg agtggccatg
     2821 cattacctac tgaaaaggaa ctttgtgcgc gctttgatgt cagtcgtatg actttgcgcc
     2881 aagctataaa attgttagcc gaagatggca tcgtagagag tacaagaggg aagggacact
     2941 ttgttattcc acaaacaaat gcccaacacg cttcaagtat ggatcaattt aagcatccac
     3001 ttgatcaaat attgctagct cgcgtatcta tgtcatcaat taattatcgt gttgatttag
     3061 aaagtgaata tacaaatcat ttgtttgcaa accatccttc tgccgtcatt gcaatggaac
     3121 gctattatca aaaaaaggac aatcattcta agcaagctga tgcattttgc tttaccttta
     3181 taccgttaaa tgtcattgac acatttaaag tgaatacaca aaacgaagac gacatgaaga
     3241 cattcgttga gcaaacgatt tatagcaatg catatcaatc agatttaaaa atgtctatta
     3301 ccaaagcgcc ccacttcaaa aatcatagtc acgtgtttga tggtgataca cattgttggc
     3361 tgattattga aacactgtac gcacaaacac cttatccaat tatgattaat aagtggtata
     3421 ttccacagga aatatctgaa ttaacactga cacgtataag acaatcggat tattaaaaat
     3481 tattaaaata taagaaattt tttgatgact atttagggag ttggtaagaa atggaaaagc
     3541 ttgttacaag cgcaatttca ttcagtcaac tactgccaat ataacattag cgagcctagg
     3601 acattgattt atgtcctagg ctcgctcaga taatcacacg atttttctat tattaatcgt
     3661 attatgttta tttatttgtt gaacgtcgtc taaatgctaa gaataaccca gatactaata
     3721 gtagtgcggc aatacttgca gtaagaatac ttgatgtcat actttcacca gcagctggta
     3781 aaactttttt gttactttgg ttcatttgat gatttgatga atgcatattt gcttttggac
     3841 tcatcatttg atggtttgat gattgcatat gaccttgttg catgtgtggc tgttggttca
     3901 tgtgtgtgtt ctgattcata tggccttgtt gactcatatg acctgtttta ttttgcacca
     3961 tttcactacc accatgatta acaccatttg catctacatt cgtttgataa aaattgccat
     4021 cttttgttaa aggtaacgca tggccattca tatctctaac agcatgtggt ttaaataccc
     4081 ctacattagt tgtaattatc acattgtatt tatcagcaat attttgcgtc gcatcataca
     4141 atttcatatt atagtcttgc atattactgc cagcgcttaa ttggcgtaaa gctttctcta
     4201 attcagtata ttgtgcttgc gtcattcctt gtggtactac tccgccataa ggacgatgat
     4261 tttgtacatt ttggctttgt ttattcgaca cattcattga agttgtgtcg gcatgcgcat
     4321 tttgaaaagg catacttgag aatactaaag tgcttgttac aattaaagaa cctactgttg
     4381 ctaatttttt cattgtcttg tccccttata ttacaatttg attacattta cattatcata
     4441 cgattacaaa ag
//
LOCUS       DAMQYT010000195         4342 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMQYT010000195 DAMQYT010000000
VERSION     DAMQYT010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.199"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(1..493)
                     /locus_tag="PGA33_002344"
                     /pseudo
     CDS             complement(1..493)
                     /locus_tag="PGA33_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            525..694
                     /locus_tag="PGA33_002345"
                     /pseudo
     CDS             525..694
                     /locus_tag="PGA33_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004399387.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
     gene            1301..1651
                     /locus_tag="PGA33_002346"
     CDS             1301..1651
                     /locus_tag="PGA33_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474137.1"
                     /translation="MKTTTQELKQYMTRLFQLSNNETWECETLEEAAENILPKRFIND
                     SPLAHLILETYTYYNNELHELSIYPFLMYSNNQLISIGYLDHFDMDFLYLTDTKNTII
                     DERHLLKEGGNNHE"
     gene            1738..2049
                     /locus_tag="PGA33_002347"
     CDS             1738..2049
                     /locus_tag="PGA33_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474138.1"
                     /translation="MKINRYITRGISEQLSLDLQILLWNMVKDRDNQLNTDYLHIFKL
                     QEDDNMLSITHEQEQPAYKLEYHYTNYIKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            2065..2568
                     /locus_tag="PGA33_002348"
     CDS             2065..2568
                     /locus_tag="PGA33_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDG6474139.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKIWDDKKPACTVITMYLHL
                     DGVLSLDLTTVLILNQLANSEQYGAVYLVNLFSNIKTPENLKHIKEPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEVMHPLNPKA
                     RQKWTLK"
     gene            2582..2806
                     /locus_tag="PGA33_002349"
     CDS             2582..2806
                     /locus_tag="PGA33_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474140.1"
                     /translation="MNHDNTIQSNWRTVASCLASQNYISIVKGLVHHFTAIDDEEILD
                     KIYEEFINDDSITTVLNNDLQTIINHYLSK"
     gene            complement(3061..4188)
                     /locus_tag="PGA33_002350"
     CDS             complement(3061..4188)
                     /locus_tag="PGA33_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HDG6474141.1"
                     /translation="MKTRAAVLHEMGAKKPYKESKPLKIEYLELDNPSEHEVLIKIHA
                     AGLCHSDLSVINGNRPRPLPMALGHEASGEVIKVGKAVTRVSEGDHVVCTFIPSCGKC
                     IPCKEGRPALCENGAISNEKGEMLEGGMRLSNDEGKVYHHLGISGFAEYSVVSENSIV
                     KIDKKIPFERAAAFGCAIITGIGAVVNTAQIRSGSNVAVVGLGGIGLNAIIGAKLAGA
                     NEIIALDINEDKFELAKQFGATATFNSSDKDIDEQIKEYIPGGVEYAFETAGVVPAMK
                     VAYQITKRGGTTVTTGLPNPKDNFSFPQVTLAAEERTIKGSYVGSCVPDRDIPRFVNL
                     YNQGRLNIDSLISEVITLDEINEGFDRLSNGEVGRIIMKMH"
BASE COUNT         1485 a          783 c          629 g         1445 t
ORIGIN      
        1 aataaattgt ccgttttata atacggttta cctatttttg tcatcttttt aggtctaact
       61 cggcagttca aatgatgctt ctgcatcatt ctctgtactc tcttatgatt aattggtgat
      121 gtataacatt gattaatcaa tgctgtaatc ttacgataac cgtaggtata gtggttagct
      181 ttacataatt caataacttt ttgtgttacg gtatcatttt tatgggtttt gtttttccat
      241 cggtaatatg ttgatttagg tatgtttaat acttttagta gcaatttgat tgaatatttt
      301 acttttaatt gatccactaa atctatgact actgttggta ccacttcctt tccaatacct
      361 tgtacttttt taaaatatcc aattctatat cttttctctt attttctaat tttaattgtt
      421 ctacttctga cagctcttct aatcctttac cgtaggtata ttgtttacca acttgttgtg
      481 aaaatctata tgctagtttt ggtaaaactg aggaaggacg ctggatagct aagaatgccc
      541 ataattatgg atttattata agatatccta aaaacaaaga aagcatcaca ggatatcaat
      601 atgaaccttg gcatttacgt tatttaggaa aaagaaggcg acaaaagtgt acaaatctgg
      661 tgaaacatta gagggatttg taggcttaaa atgatcaatt taaaataatc gaaacttcaa
      721 ctaccttatt atcgacgaag tgtattccag cacttcgtct ttatttttac cgcaaacatt
      781 ataccttact atttaataaa tctattttat atctacatac ttcctctttc tttttctaaa
      841 aatttttgag aattctcttt atcaatttca ataacactta tctaatgaaa cagtcattgt
      901 cgtaattttt tcataactac acgaggctta tattgaaatt gctaaagaac atcccacgat
      961 gattttttat cgggaaagga atgggatttg cagattagaa gaagcagaag gattgtgcaa
     1021 gcacaatccc aaaatcccgt tattctttat aaataaataa tatatataag gaagcagaga
     1081 ccccacccta tcatcaatac ttataaataa tgagttgatt gttcgtcaaa ataggcggaa
     1141 tttcgggatt tccttgtcat accaaggttt gtagcggttt tttatatgga attttctcat
     1201 attgtcaacg ggatcagtaa taaatgacaa atcaaatata tattatctca atgatgtata
     1261 tgaagtacat cactatcaca ctatctaggg aggcaatgat atgaaaacaa ctactcaaga
     1321 actcaaacaa tatatgactc gcttattcca attatctaac aatgaaacat gggaatgtga
     1381 aacactggag gaagcggcag aaaatattct acccaaacgt tttattaatg attccccact
     1441 tgcacatctt atacttgaaa cttataccta ctataataat gagctacatg aactaagtat
     1501 ctatccattt ctaatgtatt ctaataacca actcatcagt attggttatc tggatcattt
     1561 tgatatggac tttctatacc tcacagatac taaaaatacg attatcgatg aacgtcattt
     1621 actaaaagaa ggagggaata accatgaata attggatcaa agtggcacaa atatctgtta
     1681 cagtcattaa cgaagtgatt gacatcatga aagaaaagca aaatggagga aaatagtatg
     1741 aaaatcaatc gatacatcac aagaggcatt agtgaacaac tatctctaga ccttcaaatc
     1801 ttactttgga acatggtaaa agatcgagac aatcaactta atacagatta cctacacatt
     1861 ttcaaactac aagaagatga taatatgttg tcaattacac atgaacaaga acaacccgca
     1921 tacaaattgg aatatcacta tacaaactat ataaaaaatc aaaatgcatt acctaagaaa
     1981 gtctacgtca tccgagaaga tgatgtagac gctttttatt atgtgatgct tttgccagaa
     2041 gaatactaaa ggaagtgacg atatatgaat acaatcaaaa gtacgataca cacagaagcg
     2101 atatttagcg atgatgaaca acatcgctac ttactcaaaa agatatggga tgacaagaaa
     2161 ccggcttgta ctgtgataac gatgtatctt catttagatg gtgtattatc actcgatctt
     2221 actacggttc tcatcctcaa tcaattagct aattctgagc aatatggcgc tgtttatctc
     2281 gttaatcttt tctctaatat taaaacacca gagaacctta aacatatcaa agagccttat
     2341 gatgagcaca cagatataca cttaatgaaa gcaattagtg aaagtgacac agtcattctt
     2401 gcttatggtg cctatgcgaa gcgaccagtt gttatcgacc gtgtcgaaca agtgatggaa
     2461 atgttaaaac ctcataaaaa gaaagtaaaa aagctcataa atccagtaac gaacgaagtt
     2521 atgcatccac tcaaccctaa agcacgtcaa aaatggacac taaagtaaag gagtattatc
     2581 tatgaaccat gacaacacta tacaatccaa ttggcgaacg gttgctagtt gtttagcatc
     2641 acaaaattac atttcaatag taaaaggtct ggtgcatcat ttcacagcca tcgatgacga
     2701 ggaaatactt gataaaatct atgaagagtt tataaatgat gactctatta caacggtact
     2761 taacaatgat ttacaaacaa ttattaatca ttatctatca aaatgaaaac tatattctaa
     2821 taaaactaga gcatccttca cttttgcggt gaaggatgct cctatatctt tttttatcta
     2881 aaaaaactat tagtaattag ttttaaaaca tttatcttct aatatataga tatgaaatgt
     2941 aaaaatacaa ttagattggc agtcacaatg caaatgtaat atttgtcagt aatcttccta
     3001 ctcaaattgt tattcttttc atagaaatca agaggaggaa cgacttgaat tctattatat
     3061 tcaatgcatc ttcattataa ttctacctac ttcaccatta gacaaacgat caaatccctc
     3121 attaatttca tctaaagtga taacctcact gatgagtgaa tcaatattta aacgtccttg
     3181 gttgtataga ttaacgaacc ttggtatatc tctgtcaggt acacaacttc ctacatatga
     3241 tcctttaatg gtacgttctt cagccgctaa agtaacttga gggaaagaaa aattatcttt
     3301 aggattaggc agtcctgttg ttacagttgt cccccctcgt ttagtaatct gataagcaac
     3361 tttcatagct ggcactacac ccgctgtttc aaaagcatat tctactccgc caggaatata
     3421 ttctttaatt tgctcatcga tatctttatc gcttgaatta aatgttgctg tagccccaaa
     3481 ttgctttgct aattcaaatt tatcttcgtt aatatctaaa gcaataattt cattggctcc
     3541 cgctagttta gctccaataa tagcattcaa tccaatacct cctaaaccaa caaccgctac
     3601 attactacca gaacgaattt gggctgtatt caccacagca ccgatacctg tgataatagc
     3661 acaaccaaat gcagctgcac gttcaaaagg tattttttta tcaattttaa ctatagagtt
     3721 ttcagagaca acagaatatt cagcaaaacc tgatatcccc aagtgatgat atacttttcc
     3781 ttcatcatta gataaacgca tccctccctc taacatttcg cctttttcat tagatattgc
     3841 tccgttttca cataatgcag gacgtccttc tttacatggg atacattttc cacaactggg
     3901 aataaatgtg cataccacat gatctccttc gctaactctt gtaacagctt ttccaacttt
     3961 aattacttca ccggaagctt catgaccaag tgccataggt aaaggtctag gtctgttacc
     4021 attaattact gacaaatcag agtgacataa tccagctgca tgaattttaa ttaatacttc
     4081 gtgttcgcta ggattatcaa gttctaagta ttcaattttt aaaggtttag attctttata
     4141 gggcttttta gcccccattt catgtaaaac tgctgctcta gttttcactt ccaacactcc
     4201 tttaatttat atacattaag tctattctta tctgttattt tacactttaa tattattatt
     4261 tacaaaaaat tctgattatt gagattattt tatataatac caatttttaa aaatttatta
     4321 tgtaaaatca taatttagaa gt
//
LOCUS       DAMQYT010000196         4333 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMQYT010000196 DAMQYT010000000
VERSION     DAMQYT010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.200"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            36..566
                     /locus_tag="PGA33_002351"
     CDS             36..566
                     /locus_tag="PGA33_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDG6474142.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNTLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            829..3612
                     /gene="clfA"
                     /locus_tag="PGA33_002352"
     CDS             829..3612
                     /gene="clfA"
                     /locus_tag="PGA33_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HDG6474143.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSS
                     STNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSL
                     AAVAADAPAAGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLT
                     MPAYIDPENVKKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFV
                     NPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRST
                     LYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSD
                     NDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSESDSDSESDSDSDSDSDSDSDSDSASDSDSGSDSDSSSDSDSESDSNSD
                     SESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLLASIGSL
                     LLFRRKKENKDKK"
     gene            3833..>4333
                     /locus_tag="PGA33_002353"
     CDS             3833..>4333
                     /locus_tag="PGA33_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HDG6474144.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNRASAVVSGEKNPYVSESLKLTN
                     NKNKSRTVEEYKKSLDDLIWSFPNLDNERFDNPEYKEAMKKYQQRFMAEDEALKKFFS
                     EEKKIKNGNTDNLDYLGLSHERYESVFNTLKKQSEEFLKEIEDIKKDNPELKDFNEEE
                     QLKCDLE"
BASE COUNT         1593 a          682 c          875 g         1183 t
ORIGIN      
        1 ttttctataa gatagaacta aaaggagggg caaagatgca aattagacaa atacatcaac
       61 atgactttgc tcaagtggac cagttaatta gaacggcatt tgaaaatagt gaacatggtt
      121 atggtaatga atcagagcta gtagaccaaa ttcgtctaag tgatacgtat gacaatacct
      181 tagaattagt agctgttctt caaaatgaag ttgtagggca cggtttacta agtgaagttt
      241 atcttgataa cgaggcacaa cgggaaattg gattagtgtt agcacctgta tctgttgata
      301 ttcatcatca aaataaaggt attgggaagc gattgattca agcattagaa cgagaagcaa
      361 tattaaaagg atataatttt atcagtgtat taggatggcc gacgtattat gccaatctag
      421 gatatcaacg cgcaagtatg tacgacattt atccaccata tgatggtata ccagacgaag
      481 cgtttttaat taaagaatta aaagtgaaca gtttagcggg aaaaacaggt accataaatt
      541 acacatctgc ttttgaaaaa atatgatttc aagctaggat tacattaggt agagttcata
      601 ttaataataa aaaatgtttg caatcaaatc gtacgttgtc gtttgtaatt cttaaaatag
      661 caataaataa aatgtttgtt agtaaagtat tattgtggat aataaaatat cgatacaaat
      721 taattgctat aatgcaattt tagtgtataa ttccattaac agagattaaa tatatcttta
      781 aagggtatat agttaatata aaatgacttt ttaaaaagag ggaataaaat gaatatgaag
      841 aaaaaagaaa aacacgcaat tcggaaaaaa tcgattggcg tggcttcagt gcttgtaggt
      901 acgttaatcg gttttggact actcagcagt aaagaagcag atgcaagtga aaatagtgtt
      961 acgcaatctg atagcgcaag taacgaaagc aaaagtaatg attcaagtag cgttagtgct
     1021 gcacctaaaa cagacgacac aaacgtgagt gatactaaaa catcgtcaaa cactaataat
     1081 ggcgaaacga gtgtggcgca aaatccagca caacaggaaa cgacacaatc atcatcaaca
     1141 aatgcaacta cggaagaaac gccggtaact ggtgaagcta ctactacgac aacgaatcaa
     1201 gctaatacac cggcaacaac tcaatcaagc aatacaaatg cggaggaatt agtgaatcaa
     1261 acaagtaatg aaacgacttc taatgatact aatacagtat catctgtaaa ttcacctcaa
     1321 aattctacaa atgcggaaaa tgtttcaaca acgcaagata cttcaactga agcaacacct
     1381 tcaaacaatg aatcagctcc acagagtaca gatgcaagta ataaagatgt agttaatcaa
     1441 gcggttaata caagtgcgcc tagaatgaga gcatttagtt tagcggcagt agctgcagat
     1501 gcaccggcag ctggcacaga tattacgaat cagttgacga atgtgacagt tggtattgac
     1561 tctggtacga ctgtgtatcc gcaccaagca ggttatgtca aactgaatta tggtttttca
     1621 gtgcctaatt ctgctgttaa aggtgacaca ttcaaaataa ctgtacctaa agaattaaac
     1681 ttaaatggtg taacttcaac tgctaaagtg ccaccaatta tggctggaga tcaagtattg
     1741 gcaaatggtg taatcgatag tgatggtaat gttatttata catttacaga ctatgtaaat
     1801 actaaagatg atgtaaaagc aactttgacc atgcccgctt atattgaccc tgaaaatgtt
     1861 aaaaagacag gtaatgtgac attggctact ggcataggta gtacaacagc aaacaaaaca
     1921 gtattagtag attatgaaaa atatggtaag ttttataact tatctattaa aggtacaatt
     1981 gaccaaatcg ataaaacaaa taatacgtat cgtcagacaa tttatgtcaa tccaagtgga
     2041 gataacgtta ttgcgccggt tttaacaggt aatttaaaac caaatacgga tagtaatgca
     2101 ttaatagatc agcaaaatac aagtattaaa gtatataaag tagataatgc agctgattta
     2161 tctgaaagtt actttgtgaa tccagaaaac tttgaggatg tcactaatag tgtgaatatt
     2221 acattcccaa atccaaatca atataaagta gagtttaata cgcctgatga tcaaattaca
     2281 acaccgtata tagtagttgt taatggtcat attgatccga atagcaaagg tgatttagct
     2341 ttacgttcaa ctttatatgg gtataactcg aatataattt ggcgctctat gtcatgggac
     2401 aacgaagtag catttaataa cggatcaggt tctggtgacg gtatcgataa accagttgtt
     2461 cctgaacaac ctgatgagcc tggtgaaatt gaaccaattc cagaggattc agattctgac
     2521 ccaggttcag attctggcag cgattctaat tcagatagcg gttcagattc gggtagtgat
     2581 tctacatcag atagtggttc agattcagcg agtgattcag attcagcaag tgattcagac
     2641 tcagcgagtg attcagattc agcaagcgat tccgactcag cgagcgattc cgactcagac
     2701 aatgactcgg attcagatag cgattctgac tcagacagtg actcagattc cgacagtgac
     2761 tcagattcag atagcgattc tgactcagac agtgactcag attcagatag cgattcagat
     2821 tcagatagcg attcagattc cgacagtgat tccgactcag acagcgattc tgactccgac
     2881 agtgattccg actcagacag cgattcagat tccgacagtg attccgactc agatagcgat
     2941 tccgactcag atagcgactc agattcagac agcgattcag attcagacag cgattcagat
     3001 tcagatagcg attcagattc cgacagtgac tcagattccg acagtgactc ggattcagat
     3061 agcgattcag attccgacag tgactcagat tccgacagtg actcagactc agacagtgat
     3121 tcggattcag cgagtgattc ggattcagat agtgattccg actccgacag tgactcggat
     3181 tcagatagcg actcagactc ggatagcgac tcggattcag atagcgattc ggactcagat
     3241 agcgattcag aatcagacag cgattcagaa tcagacagcg actcagacag tgactcagat
     3301 tcagatagtg actcggattc agcgagtgat tcagactcag gtagtgactc cgattcatca
     3361 agtgattccg actcagaaag tgattcaaat agcgattccg agtcaggttc taacaataat
     3421 gtagttccgc ctaattcacc taaaaatggt actaatgctt ctaataaaaa tgaggctaaa
     3481 gatagtaaag aaccattacc agatacaggt tctgaagatg aagcaaatac gtcactaatt
     3541 tggggattat tagcatcaat aggttcatta ctacttttca gaagaaaaaa agaaaataaa
     3601 gataagaaat aagtaataat gatattaaat taatcatatg attcatgaag aagccacctt
     3661 aaaaggtggc ttttttactt ggattttcca aatatattgt ttgaatataa ttaataatta
     3721 attcatcaac agttaattat tttaaaaagg tagatgttat ataatttggc ttggcgaaaa
     3781 aatagggtgt aaggtaggtt gttaattagg gaaaattaag gagaaaatac agttgaaaaa
     3841 taaattgcta gttttatcat tgggagcatt atgtgtatca caaatttggg aaagtaatcg
     3901 tgcgagtgca gtggtttctg gggagaagaa tccatatgta tctgagtcgt tgaaactgac
     3961 taataataaa aataaatcta gaacagtaga agagtataag aaaagcttgg atgatttaat
     4021 atggtccttt ccaaacttag ataatgaaag atttgataat cctgaatata aagaagctat
     4081 gaaaaaatat caacagagat ttatggctga agatgaggct ttgaagaaat tttttagtga
     4141 agagaaaaaa ataaaaaatg gaaatactga taatttagat tatctaggat tatctcatga
     4201 aagatatgaa agtgtattta atactttgaa aaaacaaagt gaggagttct taaaagaaat
     4261 tgaagatata aaaaaagata accctgaatt gaaagacttt aatgaagagg agcaattaaa
     4321 gtgcgactta gaa
//
LOCUS       DAMQYT010000197         4322 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMQYT010000197 DAMQYT010000000
VERSION     DAMQYT010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.201"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            177..2975
                     /locus_tag="PGA33_002354"
     CDS             177..2975
                     /locus_tag="PGA33_002354"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDG6474145.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPADLALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            2989..4257
                     /gene="sucB"
                     /locus_tag="PGA33_002355"
     CDS             2989..4257
                     /gene="sucB"
                     /locus_tag="PGA33_002355"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDG6474146.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
BASE COUNT         1596 a          672 c          838 g         1216 t
ORIGIN      
        1 tcatttctgt tgtactccca aaatttacaa taaaatacat ctataaacct agaagaatca
       61 acgcttttgt tgattcttct ttttagcaga taaataggta aatctacttt aacaaataac
      121 taaatagtga tattattaca ttgtaagcgt ttcaacattt ttgtggaggg tgtaaaatga
      181 ctaacgaaag aaaagaagtt tcagaggctc ctgtaaactt cggtgcgaat ttaggtctaa
      241 tgttagatct atatgatgac tttttacaag atccatcatc tgtaccagaa gatttacaag
      301 tcttattcag cacaattaag aatgatgact caattgtacc agctttaaaa agtacaagta
      361 gtcaaaatag cgacggcaca attaagcgtg tcatgcgttt aattgataat attcgccaat
      421 acgggcatct taaagccgat atttatcctg taaatcctcc aaaaaggaaa catgtaccta
      481 aattagagat tgaagacttt gatttagatc aacagacttt ggaaggtata tcagcaggaa
      541 ttgtttcaga tcactttgcc gacatttatg ataatgctta tgaagcaatt ttaagaatgg
      601 aaaaacgtta caaaggacca attgcatttg agtatacaca tattaataac aataccgaac
      661 gtggttggtt aaaaagaaga attgaaacgc catataaagt aacgttaaat aataacgaaa
      721 aaagggcact attcaaacaa ttagcgtatg ttgaagggtt tgaaaaatat cttcataaaa
      781 acttcgttgg tgcaaagcgt ttttcaattg aaggggtaga cgcacttgta ccgatgttac
      841 aacgtactat tacgattgct gcgaaagaag gtattaaaaa tatacaaata ggcatggctc
      901 accgtggacg tttaaacgtt ttaacgcatg tcttagaaaa accgtacgaa atgatgattt
      961 cagaatttat gcatacagat ccaatgaaat tcttacctga agatggtagc ttgcagttaa
     1021 ctgctggatg gactggtgat gtgaaatatc accttggtgg cattaaaact actgattcat
     1081 acggtacaat gcagcgtatt gcactggcta acaatccaag tcacttggaa attgttgcac
     1141 ctgttgttga ggggcgtacg agagcagcac aagatgatac acaacgagct ggggctccga
     1201 cgactgatca tcataaagca atgccaatta ttatacatgg cgatgctgct tatcctggtc
     1261 aaggaattaa cttcgaaaca atgaacttag gaaacttgaa aggctattct acgggtggtt
     1321 cattgcatat tattactaac aatagaattg gatttactac agaaccaatt gatgcacgtt
     1381 caacaactta ttctacagat gtggccaaag gttatgatgt gccaatattc catgtcaatg
     1441 cagatgacgt tgaagctact attgaagcaa ttgatattgc aatggaattt agaaaagagt
     1501 ttcataaaga cgtcgttatt gatttagtag gttatcgtcg tttcggacat aacgaaatgg
     1561 atgaaccatc aattactaat ccagttcctt atcagaatat tcgcaaacat gactctgttg
     1621 aatatgtgtt tggtaaaaag cttgttaatg aaggtgtcat ttcagaagat gaaatgcatt
     1681 catttataga acaagtccaa aaggaactaa gacaagctca tgataaaatt aataaagctg
     1741 ataaaatgga taatccagat atggaaaagc ctgcagatct tgcattaccg ttacaagcag
     1801 acgaacaatc atttactttt gatcacttga aagaaataaa tgatgcattg ttaacatatc
     1861 cggatggctt taacattttg aaaaagttaa acaaagttct tgagaagcgt catgagccgt
     1921 ttaataaaga agatggttta gttgattggg cacaagcaga acaacttgca tttgcgacaa
     1981 ttttacaaga tggtacaccg attcgcttaa ctggtcaaga tagtgaacgt ggtacattca
     2041 gtcataggca tgccgtgtta catgatgagc aaacaggtga aacatataca cctttacatc
     2101 atgttcctga tcaaaaagcg acatttgata tacacaattc tccgctttca gaagcagcag
     2161 tagttggttt tgaatacggc tataatgtgg aaaacaaaaa aagcttcaat atttgggaag
     2221 cacaatatgg tgattttgca aatatgtcac aaatgatttt tgacaacttc ttattcagtt
     2281 ctcgctcaaa atggggagaa cgttcaggat taacattatt cttacctcat gcatatgagg
     2341 gtcaagggcc tgaacattca tcagcaagat tagagcgatt tttacaatta gctgctgaaa
     2401 ataattgcac agttgtcaac ttatctagtt caagtaatta tttccactta ttgcgtgcac
     2461 aagcggctag tttagattct gaacaaatgc gaccattggt tgttatgtca ccaaaaagct
     2521 tactgagaaa taaaacagtt gcaaaaccaa ttgatgaatt tacttctggt ggatttgagc
     2581 caattttgac agaatcatat caagcggata aggttacaaa agttattttg gcaactggta
     2641 aaatgttcat tgatttaaaa gaagcattag ctaaaaatcc agacgaatca gtattactcg
     2701 ttgcgattga aagattgtat ccattcccag aggaagagat tgaagcatta ctagcacaat
     2761 tgccaaacct tgaagaagtg tcatgggtac aagaagaacc taaaaatcaa ggtgcatggt
     2821 tatatgtcta tccatatgtt aaagtgctag ttgcagataa atatgattta agttatcatg
     2881 gcagaattca aagggctgct ccagctgaag gcgatggaga aattcataaa cttgttcaaa
     2941 ataaaattat agaaaatgca ttaaaaaata actaggggga aataagtcat gccagaggtt
     3001 aaagttccag aattagcaga atctattaca gaaggtacca ttgcagaatg gttgaaaaac
     3061 gtaggggata gcgtagaaaa aggtgaagct attcttgaat tagaaactga taaagttaat
     3121 gtcgaagttg tatctgaaga agcaggtgta ttatctgaac aacttgcaag tgaaggcgac
     3181 actgtagaag ttggacaagc aattgctatc atcggcgaag gtagtggcaa tgcttctaaa
     3241 gaaaatagta acgacaatac tccacaacaa aatgaagaaa caaataataa aaaagaagaa
     3301 acaacaaata attcggtaga taaagctgaa gtaaatcaag caaatgatga caatcagcaa
     3361 cgtattaatg ctacgccttc tgcgcgtcga tatgctcgtg aaaatggtgt gaatcttgct
     3421 gaagtaagtc cgaaaacaaa tgatgtggtt cgtaaagaag atattgataa gaaacaacag
     3481 gcaccggcat caacacaaac aacacaacaa gcatctgcaa aagaagagaa aaaatacaat
     3541 caatatccta caaaaccagt gattcgtgaa aaaatgtcac gtagaaagaa aacagctgcc
     3601 aaaaaattat tagaggtatc taataataca gctatgttaa caacatttaa cgaagttgac
     3661 atgacaaatg ttatggaatt gcgtaaacgt aagaaagaac aatttatgaa agatcatgat
     3721 ggtactaaat taggatttat gtcattcttt actaaagctt ctgtagcagc tttgaaaaag
     3781 tatccagaag ttaatgcaga aatcgacggc gacgacatga ttacgaaaca atattatgat
     3841 attggtgtag ctgtttctac agatgatgga ttattagtac catttgtaag agattgtgat
     3901 aaaaagaatt ttgcagaaat cgaagcagaa attgctaatt tagcagttaa agcacgagag
     3961 aaaaaacttg gcttagatga tatggttaat ggttcattta cgattacaaa tggcggtatt
     4021 tttggatcaa tgatgagtac gccaattatc aatggtaatc aagctgcaat cttaggcatg
     4081 cattcaatta ttacaagacc aattgcgatt gatcaagata caatcgaaaa tcgtccaatg
     4141 atgtatattg cattaagcta tgatcataga attattgacg gtaaagaagc agttggattc
     4201 ttaaaaacaa ttaaagaatt aattgaaaac ccagaagact tattattaga atcttaatcc
     4261 acaacacaaa atagtttact attcctaaaa gcgggattaa atcaataaca aacagcagta
     4321 ag
//
LOCUS       DAMQYT010000198         4318 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMQYT010000198 DAMQYT010000000
VERSION     DAMQYT010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.202"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(208..912)
                     /gene="budA"
                     /locus_tag="PGA33_002356"
     CDS             complement(208..912)
                     /gene="budA"
                     /locus_tag="PGA33_002356"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDG6474147.1"
                     /translation="MTNVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDKDFTK
                     ANIDYKDIADEIREAE"
     gene            complement(949..2613)
                     /gene="alsS"
                     /locus_tag="PGA33_002357"
     CDS             complement(949..2613)
                     /gene="alsS"
                     /locus_tag="PGA33_002357"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDG6474148.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            2846..3085
                     /locus_tag="PGA33_002358"
     CDS             2846..3085
                     /locus_tag="PGA33_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474149.1"
                     /translation="MCVVIMRDVGLIDVEDCYDIFGMWCLVNGGLKLSFYGGLKMLFY
                     VGLEFVVLWRYKKPQQDLDLTVTYTFHTYIYRLVL"
     gene            complement(3067..3249)
                     /locus_tag="PGA33_002359"
     CDS             complement(3067..3249)
                     /locus_tag="PGA33_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474150.1"
                     /translation="MKDSIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSIYLMSIFL
                     ICLGTASIIYNYKTNR"
     gene            complement(3803..>4318)
                     /locus_tag="PGA33_002360"
     CDS             complement(3803..>4318)
                     /locus_tag="PGA33_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474151.1"
                     /translation="FGTEGNRNVIDRIGKGHEKLQEIMTYQPENRKTVLIFYKIQPDN
                     IYITNQYGKEAVSRLKRILEQSIPNKEYIDLSEISLITFFKPEYNKQHVNEEFNSLSS
                     SKIKNLAKENDGIIIYLNFKDVNYQLISEGNTFFSAKPYLHCVFNKDILNMDKVDIEN
                     FFKSIEVKYSK"
BASE COUNT         1356 a          755 c          611 g         1596 t
ORIGIN      
        1 aaacataaca gcaaatctca aatatctgga acctaataaa tcccctcact actgctaata
       61 gtgaagggat ttattttatt acttagacgt aattataaat atatttgttc tattgtagtc
      121 atggttaatc atatttacaa actatcattt ctaaatcgcg cattatgaag tcaattttca
      181 taaaccgtca ttatatttct ggattcatta ttcagcttct ctaatttcgt ctgcaatatc
      241 tttatagtca atatttgctt tagtgaaatc tttatcttga attggaaaat gctgttcaaa
      301 tgtttctata ttttggattt ctactttaac atcttctact tcaaaatcta agacatgtcc
      361 accaaagtta cgatcatcat tcgcaaagtg tacatgaaat cctgctgatc cgataccatg
      421 gaataattct ggtgtaaaga aaccaacgac tgaacctttg acatacgttt cagtttgttc
      481 aggttgtctt ctagctgaat cgattaaacg tgtataaggt ggttcttgag ccggcatcat
      541 acgtacatgc atttttttaa ataagcctga aatttttact gctgaaaata aattttcact
      601 caacatcttt tccttaattt ctgcaaaaac tgcttctgaa gatttatctt tcgtttcata
      661 gcttgtatct gctacaaatt tagttacagt tgcatatggt gttaactcgt caccttttaa
      721 ttctacaaat tctttatgtt catttgcatg gtaagctttt ccatctaaaa agattacctc
      781 accgtttgaa cctgttagtg tagcgatacc taagtcacca tgttgcaata attcatttat
      841 tgatgcagtt ccttttaata atcctgccat taatgtgcct aatgtaccat gttggtataa
      901 aacattagtc ataaaatctc ccactcctct ttaacattta tctttatttt agttaaatac
      961 gtcaggtaac atatttgttg aaagtttgat attatctttg taatttacag gtatatcaat
     1021 taatactgga ccatctgttt catagccctc tttaattgcc gcttctaatt cttcttgatt
     1081 agtaactcgt aaacctttcg cgccaaatga ttctgcatat tttacaaaat ctacaggacc
     1141 gaagtctaca cctgacgaac gtttatattt catttcttct tggaattcaa ccatgttata
     1201 ttttccatca ttccaaataa gctggatgat atttaaattt ttacgtacgg ccgtttctaa
     1261 atcttgtgat gaaaataaaa agccaccatc gccagcaacg gacacaactt gcgtattagg
     1321 gcgcacaagt gcagctgaaa ttgcccacgg taatgctaca ccaagcgttt gcataccatt
     1381 gctaaataat aaatgtcttg gattgtaact tctgaaatta cgtgccatcc aaatatagtg
     1441 acttccaaca tcaactgtta cagtagtatc atcagttaat accttttgca tagattcaat
     1501 aatttccact ggatgtagaa ttccttcttc atgcgtcgct ttaataccag tttcttcatc
     1561 aatatgtgtt cttaattgtt ctaattcgtc taaatgttgt tgatttataa atggctcatc
     1621 cactttttca gaaatcattt caatcgtttt agcaatatta ccaatcaact cttttttcgg
     1681 ttgcatataa ttagtaattt cagcttgaac ttcgtcaata ttgataattt gtgtttctaa
     1741 ttctttattc cagttactag cttcgtattc aattggatca taaccgattg taacaactaa
     1801 atcactttta cgtaataatt catcaccaac ttgattgcgg aataagccca cacgaccgaa
     1861 gaaatgattt tctaattcac gactaattac acctgcacct tggaatgttt ctacaactgg
     1921 taaattcgtg cgctcaacta atttgcgaat ggcatttgtt tcttctgcac ttgaacttct
     1981 cataccagct aataaaacag gaaatgatgc atttttaatc gcttcaatga catcattaat
     2041 atcttgttca ctcggtactc ctaaatttgg tttttggcaa agtgatatag ctttagattc
     2101 aactggtgaa gaaataacgt cttgcggaat actaataaaa cttgcgccat tttttcctga
     2161 agtagcaatt cgaattgcat ttgtcataac ttctgataat gattcaggat cttgtacttc
     2221 ttcactatat tttgatgaat attttaatag cgcagcatta tcaatacttt gatgcgttaa
     2281 tcgtaataaa tcattacgtt tcacttggcc acctaacgct aatacaggat ccccttcaga
     2341 tgttgcggtt aataatccag tagttaaatt acttacacca ggaccacttg taacaagtac
     2401 tacacccggt ttgcctgtta atcttccaat accttgcgcc atcattgcgg cgttttgttc
     2461 atgacgagtg acaataagtt caggaccatc atcaattaaa gcattaaata gatagtcaat
     2521 ctttgcaccc ggaataccaa aaacatattc taccccatta tttttcaaag tatcaataac
     2581 catatcggct gcagtgtact ttttatcagt catttatatt catttccctt caaatgtgat
     2641 gtatagatgt gtattacatg taattacttt gtgatttgta atctaatgat agttacgcaa
     2701 aattaatgat gtttatcaaa attttacatg tacgattaga tttgtaatac ataaaaattg
     2761 ccttcagtac attatataac ctttgttaga tgaataaaaa tcgttatttg taatactaag
     2821 tattccaaaa atgcatgaat agattttgtg cgttgtgatt atgagggatg ttggattgat
     2881 tgatgtagag gattgttacg atatttttgg gatgtggtgt ttagtcaatg gcgggctgaa
     2941 attgtcgttt tatggtgggc tgaaaatgct gttttatgtc gggcttgaat ttgtcgtttt
     3001 atggagatat aaaaagccac agcaagactt agatctaact gtgacttata catttcatac
     3061 ttatatttat cgattggttt tatagttata aatgatagaa gcagttccta aacaaattaa
     3121 gaagatactc attaaatata tagaaaagtt atctgtttta atataaaacc taaaaagtag
     3181 aaacaccagc atcgcaacaa taaaataaac aggaataaaa gctttcttcc aaaatataga
     3241 atctttcaat taagtcactc cttattttga taacactctt aaatcatggt tcttgagtta
     3301 tcatctggtt atcatccgat agctctttcc cttgaatatc tattattaga acactatcgt
     3361 acggtctccg caagctaaat gacaatttaa taatgtacac ttttgattgt gtaagcatgt
     3421 acaaattata cgtgtaaatc aataattcat agttgaatta attctatacc tgagacatta
     3481 attcatttat actctattcc atattcccca cacaaacgca aaaaaacacc tcgacataac
     3541 atcgaggtgt tttgtatata tatcgtccga caattaacgt tttgagaatt gaggtgaacg
     3601 acgagctgct ttaagaattt gtttttttac gttcagaaaa ttttagaatt tcgcatgatt
     3661 atatagactg tcgtacacct tgatattaaa ggatttcttc aattttgaat ttcattgatt
     3721 ttcatcaaat tgaagcaaag ggtattaacg gggtattaat tttttaaaaa aatctgctgg
     3781 tcggtggatt caataattga tcttatttac tgtatttgac ttctatactt ttgaaaaaat
     3841 tttctatatc aactttatcc atatttaaaa tatctttatt gaacacacaa tgcaaatatg
     3901 gttttgcaga aaaaaaagta ttgccttcac ttatcagttg ataattaaca tccttaaaat
     3961 ttaaataaat aataatacca tcattttctt tggctaaatt ctttattttc gaagaagata
     4021 aagaattaaa ctcctcattc acatgttgtt tattatattc tggtttaaaa aatgtaatta
     4081 atgatatctc tgataaatca atatattctt tattaggtat actttgctct aaaattcttt
     4141 ttaatcgaga cacagcctct ttaccatatt ggtttgtaat ataaatattg tcaggctgta
     4201 tcttgtaaaa tattaagaca gtctttctat tttctggctg ataagtcata atttcttgta
     4261 atttttcatg acctttccct attctatcaa tgacatttct attaccctct gtaccaaa
//
LOCUS       DAMQYT010000199         4312 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMQYT010000199 DAMQYT010000000
VERSION     DAMQYT010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.203"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..868
                     /locus_tag="PGA33_002361"
     CDS             <1..868
                     /locus_tag="PGA33_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDG6474152.1"
                     /translation="SFISIKNLSQKSTIRLYVKDYAVKDVLTKQVLEKFKSLIFISGT
                     LKFNHSFEAFKQLFNKDVHFNTFEVNTSLQSAKNTSVFIPSDVASYQYKNIDEYVASI
                     VSYIIEYTTITSSKCLVLFTSYKMMHMVQDMLNELPEFEDYVVLTQQQNQNYKIVQQF
                     NNFDKAILLGTSTFFEGFDFQANGIKCVMIAKLPFMNKHNAKYWLMDSEFTSTFKEYV
                     LPDAVTRFRQGLGRLIRNENDRGIIVSFDDRLINSNYKNFFEQTLENYRQKKGDIQQF
                     GKLLRQIQKKKK"
     gene            1190..2482
                     /gene="asnS"
                     /locus_tag="PGA33_002362"
     CDS             1190..2482
                     /gene="asnS"
                     /locus_tag="PGA33_002362"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDG6474153.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            2810..3496
                     /locus_tag="PGA33_002363"
     CDS             2810..3496
                     /locus_tag="PGA33_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HDG6474154.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKELNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQ
                     AALDEANSLNKLSFKYIDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            3486..4145
                     /gene="nth"
                     /locus_tag="PGA33_002364"
     CDS             3486..4145
                     /gene="nth"
                     /locus_tag="PGA33_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDG6474155.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            4150..>4312
                     /locus_tag="PGA33_002365"
     CDS             4150..>4312
                     /locus_tag="PGA33_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474156.1"
                     /translation="MTNITKEVFDNLEQEIDLFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGN"
BASE COUNT         1621 a          574 c          775 g         1342 t
ORIGIN      
        1 tagtttcatt tcgattaaaa atttatctca aaaatcaaca atacgtttat atgtgaaaga
       61 ttacgctgta aaagatgtat taacgaaaca agtattggaa aaatttaaat cgctcatttt
      121 tatatctggt actttaaaat ttaatcattc gtttgaagct tttaaacagt tattcaacaa
      181 agatgttcac tttaatacat ttgaagttaa cacgtcgtta caaagtgcaa aaaatacaag
      241 tgtatttata ccgagtgatg tagcatctta tcaatataaa aatatagatg agtatgtagc
      301 atcgattgta agctatatta ttgaatatac gactataacg tcatcaaaat gtttagtctt
      361 atttacgagt tataaaatga tgcatatggt acaagatatg ctaaatgaat tgccagaatt
      421 tgaagattat gttgtattaa cacaacagca gaaccaaaat tataaaatag tacaacagtt
      481 taataatttt gataaggcca tactgcttgg tacgtcaaca ttttttgaag gttttgattt
      541 tcaagcaaat ggtattaaat gtgtgatgat agcgaagtta ccgtttatga acaagcataa
      601 tgcaaaatat tggcttatgg attctgaatt cacttcaact ttcaaggaat atgtattacc
      661 tgatgcagtt acacgtttta gacaaggact tggaagatta attagaaatg aaaatgatcg
      721 cggcataata gtttcatttg atgatcgact cataaacagt aattataaaa acttttttga
      781 acaaacactt gaaaattatc gtcagaaaaa aggcgacatt caacagtttg gaaaattatt
      841 aagacaaatt caaaaaaaga aaaagtgaaa tacaaagtaa aattcttgtg acatgacatg
      901 tgctatgttc tgttacaaga atttttgttt ggtcgtcaaa atttgagttt tataattaaa
      961 cttgaatgtt agcacaaaaa acgaccttca tactaataat attgaggctt tattatatat
     1021 tttgtctgaa actgatgatt gtattgtctt aatataaatt aatgttcaaa tctgtgcgta
     1081 aatcattacc atttacacgt tatcttttag tctagttcta gaacttgaaa gcggattttg
     1141 ttaaaatgaa tgagtattgt ataacgaaaa aataaatagg agaatggtta tgaaaacaac
     1201 gattaaacaa gcgaaagatc atttaaacca agacgttaca attggtgctt ggttaacaaa
     1261 taaacgttca agtggtaaaa tcgccttttt acaattacgt gatggaacag gctttatgca
     1321 aggcgtagta gttaaatcag aagttgatga agaggtattc aaacttgcga aagaaattac
     1381 tcaagaatca tctctatacg ttacaggcac aattacagaa gataatcgtt ctgacttagg
     1441 atacgaaatg caagtgaaat caattgaagt tatttcagaa gcgcatgact atccgattac
     1501 acctaaaaat catggtacag aattcttaat ggatcaccgt catttatggt tacgttctaa
     1561 aaaacaacat gctgtaatga aaattagaaa tgaagttatt cgtgcaacgt atgaattttt
     1621 caacaaagat ggatttacaa aggttgatcc accaattttg acagcaagtg cgccagaagg
     1681 tacaagtgaa ttattccata ctaaatactt tgatcaagat gcgtttttat ctcaaagtgg
     1741 tcagttatac ttagaagctg cagcaatggc acacggaaaa gtattttcat ttggtccaac
     1801 tttcagagct gaaaaatcaa aaacacgtag acacttgatc gagttctgga tgattgaagg
     1861 agaaatggct ttcacaaatc atgctgaaag tttagaaatt caagaacaat atgtaacaca
     1921 tgttgtaaaa tcagttttag aaaattgtaa actagagttg aaaattttag agcgtgatac
     1981 atcaaaactt gaaaaagttg cgacaccatt ccctagaatt tcatatgatg atgcaattga
     2041 attcttaaaa gcagaaggct ttgatgatat tgaatggggc gaagattttg gtgcgccaca
     2101 tgaaacagcc attgctaatc attatgattt accggtgttt attactaatt atccaactaa
     2161 aattaagcct ttctatatgc aaccaaatcc tgaaaatgaa gaaactgtat tatgtgcaga
     2221 cttaattgca cctgaaggat acggtgaaat tattggtgga tctgaacgtg tggatgactt
     2281 agaattgtta gaacaacgcg ttaaagaaca tggattagac gaagaagcat atagttacta
     2341 cttagactta cgtcgttatg gtagtgtgcc acactgtgga tttggtttag gtttagagcg
     2401 tacagtagca tggatttctg gtgttgaaca cgttcgtgaa acagcgccat tcccaagatt
     2461 attaaaccgt ttatatccat aagttttagg gtcgttactt aaaatatata ttatgataat
     2521 aaaaagaatc gtccagccta tattaatagc tgggcgattt attcttggaa aatgaagatt
     2581 gataacggct tatattaatc atgaacaaac ttttgtttag taactatatt tggaaaataa
     2641 atagttcatc tgattatcca taaagttcat tgttaaaatc tagtgttaaa aaatactgtt
     2701 atttaaaatt tgtttgctac tagttaaata tattaagcat tttagtcgaa aattaaagaa
     2761 aatgatttat actattaagt aatggttaaa tttaaaaggg ggcacgaaca tggataaata
     2821 tcaattaaaa gcaagacctg tagtgatacg aagagaatta ttagaccatt acagtgactt
     2881 aggtttagat gagcaagatt tagtcatttt gcttaaattg atttatgctt ccgaaacatc
     2941 aaataaacag ccttcaattg aattgcttca gaagggctca actatgcaac cacgtgatat
     3001 tacaatggtt attcaaaatt tgatacaacg tgaattgcta gaattacaag ttcaaaaaga
     3061 tgaagaaggt agatttaccg aatatatgaa tctagatcct tttttcgaaa aattaagtca
     3121 tatattgaag caacaaagta tggaaacgaa agagctaaat agtaaagaaa aatttaaaca
     3181 attatttaga gttttagagg atacatttgc aaggccgtta tctccatatg aaatagaaac
     3241 gttaaatcaa tggattgatg ttgataaaca cgatactgca attatacaag cggcattaga
     3301 cgaggcgaat agtttgaata aacttagctt taaatatata gatcgtattt tacttaattg
     3361 gaagaaaaac aatgtcaaaa caattgacga ttctagaaaa ataagagaaa aattcaataa
     3421 accgaaaatg acgcacacag ttaaaacggt acctaaattt gattggttaa acggggagaa
     3481 ccttgatggt aagtaagaaa aaagcattag aaatgattga tgttatagca aatatgtttc
     3541 cggatgcaga atgtgaatta aaacatgata atccgttcga attaactatt gctgtattat
     3601 tgtcagcgca atgtacagac gttctagtaa atagagtaac aactgagctg tttaaaaaat
     3661 acaaaacgcc tgaagattat ttggcagtca gtgatgaaga acttatgaat gatattcgtt
     3721 ctatcggtct gtatcgtaac aaagctaaga atattaagaa actttgtcaa tctttgattg
     3781 atcaatttaa tggagaaata ccacaaacac ataaggaatt agaaagttta gcaggtgtag
     3841 gacgtaaaac tgctaatgta gtcatgagtg tagcatttga tgaaccttca ttagctgttg
     3901 atacgcatgt agaacgtgtt tctaaacgct taggtattaa tcgttggaaa gataatgtga
     3961 gacaggtaga agaccgttta tgttctgtca tacctagaga tagatggaat aggagccatc
     4021 atcaactcat tttctttgga agataccact gtttagcccg aaaacctaaa tgcgatattt
     4081 gtccactatt agaagattgt agagaaggac aaaaacgtta taaagctagt ttgaaagaag
     4141 cgtgataaca tgacaaatat tacaaaagag gtatttgata atttagaaca agagattgat
     4201 ttatttgcca aaaataagac attaggttct agtgaagcaa agccctactt ggatgaatac
     4261 catagtaaaa ttattgatta ttttaagcag gttaatgaca taactggcaa ta
//
LOCUS       DAMQYT010000200         4230 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMQYT010000200 DAMQYT010000000
VERSION     DAMQYT010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.204"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(92..1900)
                     /locus_tag="PGA33_002366"
     CDS             complement(92..1900)
                     /locus_tag="PGA33_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDG6474157.1"
                     /translation="MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSLHKCKGFSFHVL
                     LSLTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGK
                     FGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANHQ
                     ITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASE
                     LASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVF
                     TSLHGTSVPIVPELLQSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELAN
                     KSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQ
                     SIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFV
                     RDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGFEGKKKI
                     NAIMTKFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEGF
                     IALRPSGTEPKIKLYVSLKCPNFDDVAQKINAMIFS"
     gene            2017..2409
                     /locus_tag="PGA33_002367"
     CDS             2017..2409
                     /locus_tag="PGA33_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HDG6474158.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEKMSLPLMWEFPGGKVEKNE
                     TEKDALIREIREEMKCDLIVGDKVITTEHEYDFGIVRLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            2411..>4230
                     /locus_tag="PGA33_002368"
     CDS             2411..>4230
                     /locus_tag="PGA33_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDG6474159.1"
                     /translation="MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKSEFDLLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEN
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKK"
BASE COUNT         1521 a          583 c          736 g         1390 t
ORIGIN      
        1 ccttcaaaac gcgaaaacat aaggattcta gtttcaaaag ggctgataag cataaaatga
       61 aatgtaatat ttcgatgtat aaaattttta attagctaaa aatcatcgca ttaatttttt
      121 gagctacatc atcaaaattc ggacatttta acgacacata taatttaatt ttaggttcag
      181 taccagaagg acgtaaagcg ataaatcctt cgtcaaataa gacacgaata acatttgatt
      241 taggagaatt aatctgcgac gttgtatctt tatccaaatg ataaacctcg ctagttaaat
      301 aatcttcaat tgctttcact ttgagtcctt gaatctcttg cggtggattt gaacggaatt
      361 tggtcattat tgcattaatt ttctttttcc cttcaaatcc ttctagcgta tgcgagaata
      421 atgtatcctc atgtctacca acagtttgat aaatctgttc taattcatct ttcaatgttt
      481 tgccatataa ttttaactca gaagcgtatt ttataatgag tggcacaatt tgtacggcat
      541 ctttatcacg tacaaaaggc tctgatagaa aaccgtaact ctcttcaaat gcgaaaatca
      601 tattttgatg atcatccagt tgtcttattt cctgagcaat aaatttaaag cccgtcagca
      661 cctctttgta ttcaacatta ttatagcgtg ctaatgactt tgttaactca ctacttacaa
      721 tagattgaat cattaagcgg tgacgtaatt gagacgtttg ttgaatacga taattaagta
      781 acagcgcacc aatttggttg ccattaaaat atgtgatatg accgtgtgca tcacgttcag
      841 caataccaag tcgatctgca tcagggtcag ttgatattaa taagtctgca tgacttttat
      901 ttgcaagttc gactgcttga tcaaaagcgc gatgatcttc aggattagca ctttgaactg
      961 agctgaaatt cggatcaggt ttacattgtg cttctacaag attaaattga ttgaaattca
     1021 aagattgtaa taactcaggt acaatcggaa cacttgtacc atgcaaacta gtgaacacaa
     1081 cttgtaaatc agatttagga atatagccaa tcatattttg aatgtgtttc atatagtcat
     1141 cagttacaga tttcggaaaa ggcttgatat aagatgtatt ttgtttagaa ataggtatat
     1201 caatctgtaa tggatcgcca acttcttcga tataacgact tgcaagctca gatgcatcag
     1261 tcgataattg cgcaccatca gaaccatata ctttgatgcc gttatagtct ttcggattat
     1321 gacttgctgt aatcataatg ccagcagtag tattaagatt gcgcaccgcg aacgataatt
     1381 ccggtgtcgt tttatatgta tcaggtaaat aaactgttat ttgatggttt gctaatacat
     1441 tagcaattat ttgggcgaat tcagttgaaa gatgtctaat atcataatga atgactattg
     1501 ttggactgtt tgtttgggca tttaaataac gcgctaaacc taatgccaat ttttcaatag
     1561 taaacttatt aagtcgacct tcaccaagac cgaattttcc gcgtataccc gcagtaccaa
     1621 atgttaattt actttcaaaa ccttctcgct gttcaatgtc agattgctgc tcataaaaat
     1681 gtttaactaa actatcatta gctcgttcta tccataattc tttatccatt gttgctaaac
     1741 atcctttcaa aatctcagtt agacttaata aaacatgaaa actaaagccc ttacatttat
     1801 gtaatgaatt ataaagaaat acgccccaaa agtaaaaaaa cacagcccca agacaatact
     1861 tttcacaagt attatataat agatgtgtat gaaaatgcat ggagtagagc gttcatgtga
     1921 atgtaaaata ttaattttta atattgattt atatatagtt ttgtaatttt tgatagaata
     1981 acaattaaga tgattttaaa ttgaagggtg agtcttatga aaaaagtaat caatgtagta
     2041 ggagctatta ttttttctga taacaaaatt ctttgtgcac agagaagtga aaaaatgagt
     2101 ctgcctttaa tgtgggaatt tcctggcggt aaggttgaaa agaatgaaac tgaaaaagac
     2161 gctttgatta gagaaattag agaagaaatg aaatgtgatt taattgttgg agacaaagtt
     2221 ataactacag aacatgaata tgattttgga attgttaggt taacaacata caaatgtact
     2281 ttaaacaaag agttaccaac tctaactgaa cataagagta ttgaatggtt gtcaataaac
     2341 gaacttgata aattaaattg ggccccagcg gatataccag ccgttaataa aattatgact
     2401 gagggataat atgagtagat tactaaatga tttcaatcaa tctttacata aagggtttat
     2461 agacaaacat atttctcata aagggaatta tacaccgaag cttttagtaa ataataaaaa
     2521 tgaaaaagtt ctttcaacaa ttatagatga acttcaaaaa tgtgaaacgt tttatttttc
     2581 tgtggctttt ataacagaaa gcggtttagc cagcttaaag gctcaactat tagatttaag
     2641 caataagggt gttaaaggga aaatattaac atctaattac ttagggttta atagtcctaa
     2701 aatgtatgga gaattactta aattaaaaaa tgtagaggta agattaactg atattgctgg
     2761 attccatgcc aaaggatata tttttgagca taaggattat agttctatgg taataggaag
     2821 ctctaattta acatctaatg cgttaaaggt taattatgag cataatgttt tactgtctac
     2881 tatgaaaaat ggcgatttag ttgatagtgt aaaaagtgaa tttgacttgt tatggcaaaa
     2941 gagtacccca ctgactgagc aatggattaa ttcatataaa gaatcatttg agtaccgttc
     3001 cttagaaaaa ttagcggagg ttgaacaaac tcaaatgctg ctagctgata aggtgaaaaa
     3061 atcagtggaa attgtaccca atttaatgca agcagaagca ttaaggtcat taaaagctat
     3121 tagggataaa gccaaagata aggcgttaat aatatctgca actggtacag gtaaaacgat
     3181 tttatgtgca ttagatgtta gagaagtaaa ccctaataaa tttttattta ttgttcataa
     3241 tgaggggatt ttaaatagag ctaaggaaga atttaaaaaa gtattaccta taaaaaatga
     3301 tagtgatttt ggattgttga caggaaaaca tagagatgtc gatgccaaat atttatttgc
     3361 aaccattcaa acattatcta gagatgataa ttttaagcaa tttgatgaaa atgaatttga
     3421 ttatattgtt tttgatgaag cacatcgttc tgctgcatct acttatcaac gagtatttaa
     3481 ctacttcaaa cctaagttca tgctgggaat gactgctaca ccagaaagat cagatgaatt
     3541 aagtatattt gaactgtttg attataatat tgcatatgaa ataaggttac aagcagcatt
     3601 agaaagtgat attttatgtc catttcatta ttttggtgtg actgattatg tacatcaagg
     3661 tattaaagaa gatgatgtaa ctaaactgag atatttaact tctgatgaaa gagttaatta
     3721 tattattcaa aagacagatt actatggata ttcaggtgaa attttacaag gattgatttt
     3781 tgtaagtagc aaaaaagaag cttatgattt agcggataaa ctaagttcaa aagggattaa
     3841 aagtgttgct ttaacaggag atgatagtgt taattatcgc caaattgtaa tagaaaaatt
     3901 aaaagaaggt aagattaact atattataac tgtagatctt tttaacgaag gtattgatat
     3961 tccagaagtc aatcaagttg taatgttgag accaacagag tctagtatta tattcattca
     4021 gcaacttggt agaggattaa gaaaaagttc aaataaagag tatgttacag taattgattt
     4081 tattggtaat tataagacaa attatttaat tccgattgcg ctttctgggg atcaatcgca
     4141 aaataaagat aattataaga agtttttaac gaataacgat tcgattaatg gagtatctac
     4201 aattaatttt gaagaagttg ctaaaaaaca
//
LOCUS       DAMQYT010000201         4190 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMQYT010000201 DAMQYT010000000
VERSION     DAMQYT010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.205"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            15..758
                     /locus_tag="PGA33_002369"
     CDS             15..758
                     /locus_tag="PGA33_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDG6474160.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIV
                     LTQIYGGQQVSPNTEKLIIIARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKGN
                     RIVAFIIATTVSSLIFALAHNDFKFIPVYFGMGVIFSLAYVWTKRLAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(783..2036)
                     /locus_tag="PGA33_002370"
     CDS             complement(783..2036)
                     /locus_tag="PGA33_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474161.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPST
                     DSKPDPNNQNPSPNPKPDPDNPKPKPDPKPDPDKPKPNPDPKPDPDNPKPNPDPKPDP
                     NKPNPDPKPDPDKPKPNPNPKPDPNKPNPNPSPDPDQPGDSNHSGGSKNGGTWNPNAS
                     DGSNQGQWQPNGNQGNSQNPTGNDFVSQRFLALANGAYKYNPYILNQINKLGKDYGEV
                     TDEDIYNIIRKQNFSGNAYLNGLQQQSNYFRFQYFNPLKSERYYRNLDEQVLALITGE
                     IGSMPDLKKPEDKPDSKQRSFEPHEKDDFTVVKKQEDNKKSASTAYSKSWLAIVCSMM
                     VVFSIMLFLFVKRNKKKNKNESQRR"
     gene            2233..2859
                     /locus_tag="PGA33_002371"
     CDS             2233..2859
                     /locus_tag="PGA33_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDG6474162.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
     gene            3220..4005
                     /locus_tag="PGA33_002372"
     CDS             3220..4005
                     /locus_tag="PGA33_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HDG6474163.1"
                     /translation="MKVQIYQLPIVFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGEYVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
BASE COUNT         1334 a          625 c          719 g         1512 t
ORIGIN      
        1 tttggaggaa aattatgaca agattatggg catcattgct aactgttatt atttatatat
       61 tgtctcaatt tttaccgctt ctcattgtaa aaaaattacc atttgtacaa tatagtggca
      121 tagaactgac taaagcagtc atttacatac aacttgttct atttttaatc gccgccacga
      181 cgattatttt aattaattta aaaattaaaa atccaacaaa attagaatta gaagttaaag
      241 aacctaaaaa atatatcatt ccatgggcat tgcttggatt tgcattggta atgatttatc
      301 aaatggtagt gagcattgta ttaacgcaaa tttatggtgg acaacaagta agtcctaata
      361 cagaaaagct aattattatt gctcgaaaaa tacctatatt tatcttcttt gtatctatta
      421 ttggtccttt attagaagaa tatgtattca gaaaagtaat ctttggagaa ttatttaatg
      481 cgattaaagg taatcgtatc gtggcattta ttattgctac aacagtaagt tcattaatat
      541 ttgcattagc acataatgat ttcaaattta ttccagttta ttttggtatg ggtgtcattt
      601 tttcattagc atatgtttgg acaaaacggc ttgctgttcc aattattatc catatgttac
      661 aaaacggatt tgtcgttata ttccaattac ttaatcctga ggctttgaaa aaagccacgg
      721 aacaagcgaa ttttatatat cacattttta ttccataata aaaaaaacga atcacacacg
      781 gattatcgtc gctgtgattc gtttttattt ttctttttat ttcgctttac aaataagaat
      841 agcatgattg aaaataccac catcatagaa catacaattg ctagccaact tttactatat
      901 gcagttgacg cacttttctt attatcttct tgttttttaa ctactgtaaa atcgtctttt
      961 tcatgcggtt caaatgagcg ttgttttgaa tccggcttat cttcgggctt tttcaaatct
     1021 ggcattgatc caatttcacc agtaattaat gcgagtactt gttcatctaa attacgatag
     1081 tacctttctg atttcaatgg attgaaatat tggaatctaa agtaattcga ttgctgttgt
     1141 aatccattta aatatgcatt tccgctgaaa ttttgttttc gaataatatt ataaatgtct
     1201 tcatcagtaa cttctccata atctttgccc aacttattaa tttgatttaa aatatacgga
     1261 ttatacttgt aagccccatt tgccaaggct aaaaatcgtt gggatacaaa atcattacca
     1321 gtaggatttt gtgagtttcc ttgattccca tttggttgcc attgaccttg attagatcca
     1381 tctgaagcat ttgggttcca tgtcccccca tttttcgagc caccagaatg attggaatcc
     1441 ccaggttgat cgggatctgg tgacgggtta ggatttggct tattagggtc tggttttgga
     1501 ttcggatttg gctttggttt atctggatct ggttttggat ccggatttgg cttattaggg
     1561 tctggttttg gatctggatt tggtttcggg ttatctggat ctggttttgg atccggattt
     1621 ggctttggtt tatctgggtc tggttttgga tccggttttg gtttcgggtt atctggatct
     1681 ggtttaggat tcggacttgg gttttggtta tttgggtctg gttttgaatc tgtacttggg
     1741 ttttggttgt ttgagtctgg cttcacatta ttcgaatcag gattagcatc acgactattc
     1801 gaacctgttc cttcattacc cgtttgattt gattcgtgag gtgtcccagt ctcattatta
     1861 ttcatttcac tatttactga tggtgaagtt atattatgat tcaaattagt agttggtttt
     1921 tctccattta agttatcctt tgcttcaact gtgtgcgttg caagccctaa actactacta
     1981 cttaaaatta aaatacttgt tgaaagtaac attttcttaa accaatgata tgacatttca
     2041 cgagacacct ccctttttca tgtaagtatt aatctaagta taataaatat gcgttgaaaa
     2101 tgcatttata tttggaattt gaaaatgaat attaatattc ttgaaatttt cgtaaatatt
     2161 gctttacaag tacgagtata aattttatac ttagtctatt aagtattctt tactaaagaa
     2221 ggagcaataa ttatgggctt atttaaaaaa gataaaaaag caatgacttt tgacaatgca
     2281 atggaagaaa gacgttcaat atataatttg aaagattcga tatcaattag cgatgacgaa
     2341 ttagaatcaa tgattgcaca tgcagttaaa catgttcctt catcatttaa ttctcaatca
     2401 actagaattg ttctcttgtt aaatgacaac aataataaat tttgggataa cacaaaagca
     2461 attttaaaag aagtcatggg agagaatcgt gatttcgagc caactgaaca aaaaattgat
     2521 aacttcaaac attcatacgg tacaattttg ttttatgaag atcaagatgt tgtaagtggt
     2581 ttacaagaac aaatgccaaa ctactatgat aattttgcaa tatggtcaac tcaaacaaat
     2641 gccatgcatc aatttgcaat ttggactgca ttagctacta aaggcatagg tgcttcatta
     2701 caacactata atccacttgt tgatgagatg acttcaaatg aatttaatat tcctaaaagt
     2761 tggaaactta ttgctcaaat gccatttggt gatattcgtg aagctgctgg cgaaaaaaca
     2821 tttaatccag tggaagatcg tttcgtaata aaaaaataat caatatattg ttaggaatgg
     2881 gacagaaatg ataaagaatc attagttatt cattatgtag tagttcttac acattagccg
     2941 ctgctaatgt gcacttaaga ataggaatac atgagtaaaa ctcatgtata agaaatacta
     3001 atttctaaag aaaaagtatt tctttatgtt agagcccacc ccaacttgca ttgtctgtag
     3061 aaattggtgt tccaatttct ctatgttggg gccccatcaa ctactgtcaa tataactttg
     3121 tagagcctag gactttgatt ttatgtcttg tgctcgtttt ttgcgtttca acataacttt
     3181 tagtaagtta tatataagat ctatcaagga tgtgatgtta tgaaagtcca aatttatcaa
     3241 ttaccgattg tttttggaga tagttctaaa aatgaaactc aaataacgca atggttcgaa
     3301 aaaaatatga atgctgaagt agatgtagtc gttttacctg aaatgtggaa taatggctat
     3361 gatttagagc atttaaatga aaaagcagat aataatctag gacaaagttt ttcatttatt
     3421 aaacatttag ctgaaaaata taaagtcgat atcgtagctg ggtcagtttc taacattaga
     3481 aacaatcaaa tatttaatac agcttttagt gtgaataaaa gtggccagct gattaatgaa
     3541 tacgacaaag ttcatttagt tcctatgtta agagagcatg aatttttaac cgctggcgaa
     3601 tacgtagctg aaccctttca attgtctgac ggtacttatg tgacccaact tatctgttat
     3661 gatttacgtt ttccagaatt attaagatat cctgctcgta gtggtgctaa aattgcattt
     3721 tatgtggcgc aatggcctat gtcacgtctt caacattggc attcattact aaaggcacgt
     3781 gcaattgaaa ataatatgtt tgtcattgga actaatagca ctggattcga tggtaacaca
     3841 gaatatgctg gtcattcgat agttataaac ccaaatggag atttagttgg agaattaaat
     3901 gaatccgcag atattttgac tgtagattta aacttaaatg aagtagaaca gcaacgcgaa
     3961 aatatacctg tatttaaaag tattaaatta gatttgtata aataagaagc gcccgaaata
     4021 atatttaaca catgtcatga ctaaacatag atttatgaga gatttttcat aagtctatgt
     4081 ttattttttt ataatagata actcagtaag aacccatttc gccctttgca aatgaatgat
     4141 tttcttaatt aaattgatta attttttgac cttttccaac attagactta
//
LOCUS       DAMQYT010000202         4108 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMQYT010000202 DAMQYT010000000
VERSION     DAMQYT010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.206"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            23..1654
                     /locus_tag="PGA33_002373"
     CDS             23..1654
                     /locus_tag="PGA33_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDG6474164.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGIIVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNYNNEFLLKYEENQKPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            1651..3324
                     /locus_tag="PGA33_002374"
     CDS             1651..3324
                     /locus_tag="PGA33_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HDG6474165.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKKRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSVRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAITRMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(3451..3894)
                     /gene="mgrA"
                     /locus_tag="PGA33_002375"
     CDS             complement(3451..3894)
                     /gene="mgrA"
                     /locus_tag="PGA33_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HDG6474166.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
BASE COUNT         1372 a          577 c          729 g         1430 t
ORIGIN      
        1 aataggttta ggtgatataa ttgtgaaaaa attaacaaca atactgtttc aatataaaat
       61 ttttccggta ctcatgttct tggtcagtac tggtctcggc ataatcgtta taacgcaaaa
      121 tattttaata gcagattttt tagctaaaat tataagacat caatttcaag gtttatggat
      181 tgtattattt attttattag gtgttttact tttaagagca actgtgcaat ttctaaatca
      241 atggttaggt gatacattag catttaaagt taagcatatg cttagacagc gggttattta
      301 taaaaataat ggtcatccaa tcggtgaaca aatgactata ctcacagaaa acattgatgg
      361 tctagcacct ttttataaga gttatttgcc tcaagtgttc aaatcaatga tggttccgct
      421 catcataatc attgcaatgt ttttcatcca tttcaatacc gcattaatta tgttaataac
      481 tgcaccattt attcctttgt tttatattat tttcggtttg aaaacgcgag atgagtcaaa
      541 agatcaaatg acttatttga atcaatttag tcaacggttt ttaaatattg ctaaaggttt
      601 agtgacgtta aagctattta atcgtacaga gcaaacagag aagcatattt acgacgatag
      661 tactcagttt agaactttaa caatgcgcat tttacgcagt gcttttttat cgggattaat
      721 gctcgaattt ataagtatgt taggtattgg attggttgca ttggaagcaa cgctaagctt
      781 agtagtattt cataatattg attttaaaac tgcggcaatt gcgattattt tagcgcctga
      841 attttataat gcaattaagg acttagggca agcgttccat actggaaaac aaagtgaagg
      901 tgccagtgac gttgtgtttg agtttttaga acaaccgaac tataataatg aatttctatt
      961 aaagtatgag gaaaaccaaa agccatttat tcagttaaca gacatatcat ttcgatatga
     1021 tgattctgat agattggtat taaatgattt aaatttggaa atatttaaag gtgatcaaat
     1081 tgcacttgta ggtccaagcg gggcaggtaa atccactttg acacatctta ttgcaggtgt
     1141 ttatcagcca acaataggta ctataagtac aaaccagcgt gatttaaata taggaatact
     1201 tagtcaacag ccatatattt tcagtgcttc tataaaagag aatattacga tgtttaaaga
     1261 tatagaaaat aatactattg aagaagtgct agacgaagta ggtttattag acaaagtgca
     1321 atctttcaca aaaggcatta acacaataat aggtgaagga ggcgaaatgt tatctggtgg
     1381 acagatgaga cgcatagaac tttgccgtct tttagttatg aagccagatc tcgttatatt
     1441 tgatgagcct gcaactggtt tagatattca aacagaacac atgattcaga acgttctgtt
     1501 tcaacatttt aaagatacaa cgatgattgt cattgcacat agagataata caattcgcca
     1561 tttacaacga cgcttgtata tagaaaatgg aagactgatt gctgatgatc gcaatatttc
     1621 agtaaatata acagaaaatg gtgatgactt atgaaaacac gactaaaatt tcaagtagat
     1681 aaggatttat tgttagctat agttgttggt gtttgtggaa gtttagttgc gctcgccatg
     1741 tttttcttaa gtggttatat ggtgacacaa agtgcacttg gtgcgccact atacgctctg
     1801 atgattttag tcgttacagt aaaattgttt gggtttttaa gagctattac tcgatacgta
     1861 gagcgcctta tttctcataa agctacattt acaatgctac gtgatattcg ggtacagttt
     1921 ttcggtaaat tagtaaatgt cattcctaat gtttaccgta aactgagttc tagtgattta
     1981 atttcacgta tgattagtcg tgttgaggca ttacaaaata tatatttacg tgtttattat
     2041 ccaccagtcg tcatcggttt gacagcgcta gttacagtca tagttttggc gttcatttca
     2101 atcggccatg cgctattgat tatggttagc atgttgttca ctttactcat tgttccttgg
     2161 ttaagctcaa aaaaagcacg tactttaaag aaacatgcag ctaatgaaca ggcccgattt
     2221 ttaaatcatt tttatgatta taaagctggt atggatgaac tacgtcgatt taatcaaatt
     2281 aatcattatc gagataattt gatggctaaa ttaaatcatt ttgataaatt acaacttaaa
     2341 gagcaacgct ttttaacgat ttatgatttt atattaaata ttattgctat gctttcgatt
     2401 tttggtagtt tagttctagg attaattcaa attaatgcag gccaactaaa tattatttat
     2461 atgacgagta tagttttaat ggtcttaact ttatttgaac aagctgtacc aatgacaaat
     2521 gtcgcgtatt ataaagcgga tactgaccaa gcattgcacg atattaatga agtgatatct
     2581 gtaccttcta ctaatggaaa aaaacgtctt aatgataagt atgatgcaac gaacatttat
     2641 gaagttaagg atgctagttt taagtattgg aatcagcaaa cgtatgtgtt gtcggatatt
     2701 aattttaatg ttaatagagg cgaaaagatt gcgattgtgg gtccttctgg ttcaggaaaa
     2761 agtacattac tacaaattat ggcagggtta tatcaattag atagtggctc tgttcgtttc
     2821 gaaaatatgg atatgtttga aatagatgac aaagataagt ttgaatcgtt aaatgtcttg
     2881 ctacaatctc aacaattatt tgatggtaca atacgtcaaa atttatttac cgatgaaaaa
     2941 gatgaagcgg tgcaagcaat atttaagcaa ttagatttag aacatttggc actagaacgt
     3001 caaattgact tagatggtca tacattatct ggcggagaaa ttcagcgttt agcgattacg
     3061 aggatgttat taaaagatac tgcatcaaca tggattttag atgaaccaac aactgcatta
     3121 gataaacaaa atagtttaaa agttatggat ttaattgaag cacatgcaga aacattaatt
     3181 gttgctacac acgatttaac tttattgtca cgttttgaga ccatcattgt gatgataaat
     3241 ggtaaaatag ttgaaaaggg aaactatcaa caattactag ctaatcaagg tgctttatgg
     3301 aatatgattc aatataatgc ataaaaaaac tgcttgatag gctaagaaac ctttcaagca
     3361 gttttttaac taacaagctt acgcgtgcta attgtttttc tattcttaat aaattctaaa
     3421 cattacttta attgtcatga caaaagttaa ttatttttcc tttgtttcat caaatgcatg
     3481 aatgacttta cctaataagc gattaagttc tttaacttca tcttgcgata aagaagaagc
     3541 tgaagcgact ttgtcagatg cattacttaa ttctggtcta atagtttcac ttttgtcagt
     3601 caagtgaata aatacttcac gttgatcgac ttcggaacgt tcacgcttaa ttaagtctac
     3661 ttgttccatt cgttttaata atggtgatac tgtaccagta tcgagtgcta attcagttac
     3721 gactttcttg acgtttacag gagattcatc ccataaaatt gttaagacaa gaaattgtgg
     3781 gtatgttaga ttgtacttct taaaaacttt gttagagtag tagcgattaa cttgtctttg
     3841 agcattgtac aaactaaagc atagctgttc ttttaaatta tgttgatcag acattaaagt
     3901 tctcctccag acatactatc cgtttttttc tcttttcgga ttggtaatca ttaaaaagtt
     3961 gattgtttat taattcacaa ctttctttga ttcaatgcca tgctaaaatt aaagtatgtt
     4021 taaagtttag aagatatttt tgattaaatc aagcaaaaag ataatttaat atatatgtta
     4081 tcatttttaa aaataactgt aatagaaa
//
LOCUS       DAMQYT010000203         4102 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMQYT010000203 DAMQYT010000000
VERSION     DAMQYT010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.207"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..645)
                     /locus_tag="PGA33_002376"
     CDS             complement(<1..645)
                     /locus_tag="PGA33_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDG6474167.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQT"
     gene            complement(795..2468)
                     /locus_tag="PGA33_002377"
     CDS             complement(795..2468)
                     /locus_tag="PGA33_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDG6474168.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            complement(2626..>4102)
                     /gene="glpK"
                     /locus_tag="PGA33_002378"
     CDS             complement(2626..>4102)
                     /gene="glpK"
                     /locus_tag="PGA33_002378"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDG6474169.1"
                     /translation="IDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWVEHDANEI
                     WTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVWQSRQTQS
                     ICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLLFGTIDTW
                     LVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKASSEVYGK
                     TIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGDKAVKSES
                     GLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATRVDSTEGV
                     YVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVMEAMSKDSG
                     IDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAVGFWESKD
                     DIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
BASE COUNT         1223 a          857 c          558 g         1464 t
ORIGIN      
        1 tgtttgatat aataattgat ttgacacatt aaatccacta tatggatctt taataaagtt
       61 atctacttca attggattac tagaaatcca atcactttgt gtacgtaatg gacgtatttt
      121 tttattaaaa ctatttgata ctaactggtt aacccattca actcgtttct cagcaccata
      181 aatttttgta atcagttgta acactttaac ggttggtaaa cctttccata atgaatacat
      241 accagtacca cttaaaatta gaccatcaac atattgcgga tatgtttcta caaacaatct
      301 agctataacg gatcccattg aatgtccgat tataatgtat ggtttatcaa cattgcctct
      361 cactgtttgc gctacttcaa aggcatcacc gataactcgt ttcatatcat cgtaatgccc
      421 tcttgttgat tcatcaatat taataccatg tcctcgatga ttatgacgta tcacatcgaa
      481 gccatgcttt gaaagtgcat gtgctaattt atcgtaacgt tccatatgtt cagccatacc
      541 atgaaatata tgaataattc cttttgaatc tacatctgta aaattcactt taacttcaat
      601 attcgtatta tcttgtacag ttaatttaaa ttgtttttca gtcatcatct cacaccttta
      661 ataacaacgc ttttatattc gcttttatta ttatcataca tttagtgatg catagtttca
      721 attgactaat gtatctcgta taaaaaaact gatgccctta aaacaggaca ccagtttatg
      781 attgtacaaa ttaattattc tttaactgct ggttgattat ttccatgttg cgcttcttta
      841 attgcttgct caacttcttc agtatatgct tcaatttgag ctggagagta atcaagcata
      901 tctgccataa tatcgatgac agcatcttta taatctaata catctttaat attgaaatac
      961 attttaccag aacgacgaac taagaaatcg ttaggtttgt ataccatttc ttgttgaata
     1021 ctataaacaa gttctacata aatttctaat ggtaacttgc tatcatggta ttgagatgtt
     1081 tgcgcaatgt tgaacaattc atcaacattt gaaccatatt tagatgctaa acgtcttgca
     1141 acatcttcat caatgccgaa tcctttagct acatctactt tttgctctac aaacgcatca
     1201 aagttcttgc taccacctac atcgccacct gaaattgcca gaccttttgt attacatgga
     1261 ctaaatgtta aaccgtagtc ttttttcaag cgtttagata ctaaatcaac aatgtcttga
     1321 gccatgtgac gatagcctgt taatttacca cctgcaatag ttaataaacc tgatttacct
     1381 tcccaaattt catccttacg agagatttca gaagggtctt tgccttcttc gtaaattaat
     1441 ggtctaattc ctgcccatgt tgattcaata tcttcatctg taacattaac actagggaac
     1501 atgtaattaa tcgcatcgat taaatagtct ctgtcttctt gtgtagttaa tggtgaagat
     1561 ttgatattgt catagaatgt atctgtagta cctacatacg cttttccttc acgtggaatt
     1621 gcaaaaatca ttcttccatc tttttcagta tcaaagtata ctgcttgacc taatgggaat
     1681 tttgattgat caataacaac atgtacacct ttagttaaac gtaatttttt attattgcgt
     1741 gcataatcac cacttctaac atcatcaacc catggacctg ctgcattaac cactttttta
     1801 gccttaattg tataattttc attagttaat ttatctataa ctttaacacc atttacttgt
     1861 tgatttttat cataagtgaa gtgttcagat ttagtatagt tgataatttc tgcgcctttt
     1921 tcagcagcac gcttcataac ttcaatagtt aaacgcgcat cgtcagtacg atattcaaca
     1981 tagtaaccgc cgccttttag accttctttt ttaactaatg gttctttagc taaagtttct
     2041 tttttagata acattttttt acgttcagac ttcttaacac ctgctaaacg atcatacatt
     2101 cctaaaccaa ttgatgttga gaatttacca aatgttccac ctttatgcat tggtaaaagc
     2161 atccactctg gagtcgtaac atgaggccca ttttcataaa caatcgcacg ttctttacca
     2221 gtttcggcaa ctactccaat ttggaattgt tttaagtaac gcaaaccacc atggactaat
     2281 tttgtagatc ttgagcttgt tccttgtgca aagtcttgca tttcaactaa tgcaactttc
     2341 attcctcttt cactcgcgtc tagtgcaata cctgcacctg taataccgcc accaataatt
     2401 actaaatcat attcatcatt tcttaaattc tttttaatat gttctctctt aaaagtagac
     2461 aatgccattc caaaacgcct cctaaaaaat gtaaaaacga gagacttgtc ccacataaac
     2521 aatccttcaa ttgtacatgt tcgaacaaat ctctcgcttc tcgattcaaa tattaactta
     2581 aattgattgt atcacaatgt ttatacaaaa gtctaatcta caagtttatt ctgttttaaa
     2641 aacttgtgtt gcttcaacag cttttttcca acctctatat aatttttctc tttcgccttc
     2701 atccattttc ggatcgaatt tttcttctaa tttccagttt ttagcgatat catctttact
     2761 ctcccagaat ccaactgcta aacctgctaa aaatgcagca cctaaagctg tagtttcttg
     2821 aatttcaggt ctttcaacag aagtattaac aatgtctgct tggaactgca taataaagtt
     2881 atttttaact gcaccaccat cgacacgtaa actttggaca tcaataccag agtcttttga
     2941 cattgcttcc ataacgtcac gagtttggta acatagtgat tctaaagttg cacggataaa
     3001 gtgctctttt tcagttccac gtgttaaacc gaaaatcgca ccacgtgctt cagaatccca
     3061 atatggtgtt cctaaaccta caaaagctgg aacaacataa acaccctcag tagagtcaac
     3121 tcgtgtcgca taactttctg attgtggtgc tgaattaatc attcttaatc catcacgtaa
     3181 ccattggatt gctgaacccg aaacaaagat ggaaccttca agcgcataat ttacttttcc
     3241 atcaatacca taagcaattg ttgttaataa accactttca gatttaaccg ctttgtcacc
     3301 tgtattcatt aacatgaagc caccagttcc atatgtgttt ttcacgtcac cacgttcgaa
     3361 gcaagcttgt ccaaataatg ctgcttgttg atcaccagct actccagcga ttggtacttc
     3421 ttgaccatag aagtggtaat caattgtctt accatatact tcactcgaag ctttaacttc
     3481 tggcaacata tttttaggta ctgtaagtag ttctaataac tcatcgtccc attctaaatc
     3541 atggatatta aacattaatg tacgactcgc atttgaataa tcagtaatat gcgcagcttt
     3601 tcctgataat ttccatacta accaagtatc aatcgttcca aataatagat cgccattttc
     3661 tgctttttct cgtgcacctt caacattgtc tagaatccat ttaacttttg tacctgcaaa
     3721 atacggatct aaaagtaatc ctgtcttatc tctaaatgtt tgttcatatc cttgttgttt
     3781 taattctgaa caaattgatt gtgtttgacg tgattgccaa acaattgcgt gataaattgg
     3841 gcggccagta tgtttgtccc aaacaaccgt tgtttcacgt tggtttgtaa taccgatacc
     3901 tgcaatttga tcagctctaa catcattttc attaattact tccgtcatta cagctaacac
     3961 agatgtccaa atttcatttg catcatgttc aacccaacct gattgtggaa aatattgctt
     4021 aaactcacgt tgtgctaccc ctgcaatttc ccctttttga ttgaataaaa tcgctcttga
     4081 gcttgttgtt ccttggtcta ta
//
LOCUS       DAMQYT010000204         4062 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMQYT010000204 DAMQYT010000000
VERSION     DAMQYT010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.208"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..2763
                     /locus_tag="PGA33_002379"
     CDS             <1..2763
                     /locus_tag="PGA33_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HDG6474170.1"
                     /translation="LKYLIRMSTRPTPFGLLSGINLGHFVNEPTRLKVGNSIQKYVKV
                     DGEWLYKLVSYIESIDEYYQNLKVIWNSKAHIINDRIYLNEQSAIYLNNNKDTSFSIK
                     NSELLVFIKTTVTNNNITFSNLAEKINQEFEINDITKVKVYIHNLVSKEIIYSTIRPP
                     LSYSDNLNYILNKLSLHNDDFVKKIREIQKLILAYEKTEIGFGEELYKDIIHHMKALF
                     KCKNYLQIDTKIDMINNYLHQDIATNISEAAYLLWLLSRNNIGFTDLKVLHNRFIEKY
                     GFEQLVNIKDLISDITGFGTTIFQEEETDGNNIVMLKQKFLHALRNNDEIVINEKDVE
                     SLINDNEINHYHAPMSADVYAELYLGRFYNQYNELIVISPLTASFNAGATFGRFHHLI
                     DTETLAKLEHEKGHYYQKMICDDNVEMISINNIPKYPRNHNVLTNHDSYEYSLNLGSS
                     NSYSKYELTLDDIYVGATFNKLYLYSSQLNKRVLFESNNMYNFLKECNLYRLLREISM
                     ESVKCIEPMNDVSIDSFSYSPRIRYKNVILKPAYWKINEMVLPLPKNEEWDQQFLKYQ
                     EQFNIPNIVNLVYGDNKLLLNLSLANHRYLLMKEYKKHKRVRLVESFLPQSKNDHVYE
                     IVTPIYKKSSYRGPEIEIPKYKNTDIEYDKDWFALHIHIDKPSQDTFIIDNLYPFVKH
                     LKDKGDIDQYFLMRYIKQGDILKLRLFRNDENYAEIYSILKNWLPHVRQTTEVSDYEF
                     VSYEPEFFRYGGKNTINEIEAFFEYDTNLAVNIIENDFKFDRPYIVAISIMYLFEMFS
                     ISNEERMEIVNNYVPTSFKSKDIRPFKNELVTICNPANNFEYMAKHYSGIYRILKDGN
                     QILSKLNEGLKKTLTTKRSRIIGSLIHMRCNRIFGIDKDQETFVLSIVKEIVKTQKHW
                     CGDKND"
     gene            2756..4000
                     /locus_tag="PGA33_002380"
     CDS             2756..4000
                     /locus_tag="PGA33_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HDG6474171.1"
                     /translation="MIDMNLILKKKFQEISEVDDFISKASTETDYFEPSTLSHGIPGI
                     ILFLDAYQKVFDINTEQIVHKYIMKLAPYLQSGQYNNSLFGGLSGIAFSMDLASQNGK
                     NYQNILNNIDGHIVNEIENKFDQILQEPLNPLNYDTVSGLAGIGRYLLNRVDENEFNV
                     KALKSILVYFKDIQYSKNSWVVPQESQFLESDKNYFTEGNINLGLAHGVLGPMSLFAL
                     CVIKGITIENHQHILKDMYKFIMDEKFCNHERWLQRYDLISERNHFNFIRNGWCYGNT
                     GVMTTLFLIGQALQDDEIIKMSKKVMLQVVNDKDENLISPTICHGLSSQILMLTIMNL
                     NFELNEVSDYITVLINKLISHYKEDYLVNFIDINENKQDVFKSRKVGLLEGELGVYLT
                     LMTLKNTKILNEKNWTNAFLIS"
BASE COUNT         1561 a          486 c          658 g         1357 t
ORIGIN      
        1 ttaaaatatt taattagaat gtcaacacgt ccaactccat ttggattatt aagtggtatt
       61 aatttggggc attttgtaaa tgaaccaaca cgtcttaaag ttggaaatag tattcaaaaa
      121 tatgtgaaag tcgatggcga atggttgtat aaactggtta gttatattga gagtattgat
      181 gaatattatc aaaatttgaa agttatttgg aatagtaaag cacatattat caatgatcga
      241 atatatttaa atgaacaaag tgctatttat ttaaacaaca ataaagatac atctttttcc
      301 attaaaaata gcgaattact cgtattcatc aaaaccactg tgacgaataa taatattact
      361 ttttcaaatt tagctgaaaa gataaatcaa gaatttgaaa ttaatgatat tactaaagtg
      421 aaagtatata tccataatct tgtctcaaag gaaattatat attcaacaat cagaccaccg
      481 ttatcatata gcgataattt aaattatatt ttaaataaac tttctttaca taatgatgat
      541 tttgtgaaaa aaattagaga aatccagaaa ttgatattag cttatgaaaa aactgaaatt
      601 ggatttggcg aggaattata taaagacatt attcatcata tgaaggcact attcaaatgt
      661 aaaaactatc ttcaaattga taccaaaatt gacatgatta acaattattt acatcaagat
      721 atagcaacaa atatatctga agcagcgtac ttattgtggc ttttatcaag aaataatatt
      781 ggattcacag atttgaaagt tcttcacaac cgttttattg aaaagtatgg atttgaacaa
      841 ctcgtgaata ttaaagattt gatttcagat ataacaggtt tcgggacaac aatttttcaa
      901 gaagaagaaa ccgatggaaa taatattgta atgcttaagc aaaaattttt acatgccttg
      961 agaaataacg atgagatagt cataaatgaa aaagatgtag agtcattgat taacgataat
     1021 gaaatcaatc attatcatgc gccaatgagt gcagatgttt atgctgaact ttatttagga
     1081 cgtttttaca atcagtacaa tgagctcatt gttattagtc ctttaacggc gtcttttaat
     1141 gctggcgcta catttgggcg atttcatcat ttaattgata ctgaaacttt agcaaaatta
     1201 gaacatgaaa aaggacatta ttatcagaag atgatatgtg atgacaatgt agaaatgatt
     1261 tctataaata acataccgaa atatccgaga aatcataatg tattaactaa tcatgactca
     1321 tacgaatatt cattgaattt aggaagtagt aatagttatt caaagtatga gcttacctta
     1381 gatgatattt atgttggtgc tacctttaac aaattatatt tatattctag ccaactaaat
     1441 aaaagggtac tatttgaatc aaacaatatg tataactttt taaaagaatg taatttatat
     1501 cgattgttac gagaaatttc aatggaatct gtgaaatgca ttgaacctat gaatgatgtg
     1561 agtattgatt catttagtta ttcaccacga atcagatata aaaatgtcat tcttaaacca
     1621 gcatattgga aaattaacga gatggtatta cctttaccta aaaatgaaga atgggatcaa
     1681 caatttttga aatatcaaga acaattcaat atacctaata tcgtgaattt agtttatgga
     1741 gataacaagc ttctactaaa tttatcatta gctaatcata gatatttatt gatgaaagaa
     1801 tataagaagc ataaacgtgt acgtttagta gaatcatttt tacctcaatc aaagaatgat
     1861 catgtgtatg aaattgttac accaatttat aaaaaatcat cttatcgagg acccgaaatc
     1921 gaaattccaa aatataaaaa tacagatatt gaatatgaca aagactggtt tgcattacat
     1981 attcatatcg ataagccatc gcaagataca tttatcatcg acaatttgta tccatttgta
     2041 aaacatctta aagataaggg agatatagat caatattttt taatgcgtta tatcaaacaa
     2101 ggtgacattt taaagttgag gttattcagg aatgacgaaa attacgcaga aatatacagc
     2161 atcttgaaga attggttacc acatgttcga caaacgacag aagtttcaga ttatgaattt
     2221 gtatcatacg agccagaatt ttttagatat ggtggaaaga acacgattaa tgaaatagaa
     2281 gccttctttg aatatgacac gaatttagct gtcaatatta ttgaaaatga ttttaagttt
     2341 gatcgaccat atattgttgc tatttcaata atgtatttat ttgagatgtt ttcaatttct
     2401 aatgaagagc gtatggaaat tgttaataac tatgtaccta catcatttaa aagtaaagat
     2461 attagacctt ttaaaaatga acttgtaact atatgtaatc ctgctaataa ttttgaatat
     2521 atggctaaac actatagtgg tatttatcga attttaaaag atggcaacca aattttgagc
     2581 aaattaaatg aagggcttaa aaaaacttta actactaaaa ggtcaaggat tattggcagc
     2641 ttaattcata tgcgctgcaa tcgaatattt ggcattgata aggatcaaga aacatttgtt
     2701 ttatctattg taaaagaaat tgttaaaact caaaagcatt ggtgtggtga caaaaatgat
     2761 tgatatgaat ctaatattga agaagaagtt tcaagagatt tcagaagtag atgatttcat
     2821 ttcaaaagca tctactgaaa ccgattattt tgaaccttca actttatcac atggtatacc
     2881 aggtatcatt ttgtttttag atgcttatca aaaagttttc gatattaata ctgagcaaat
     2941 tgtgcataag tacatcatga aattagcacc atatttacag agtggtcaat ataacaattc
     3001 tttgtttggt ggactttcag gtattgcatt ttcaatggat ttagcatcgc aaaatggcaa
     3061 aaattatcaa aatattttaa ataatattga tggtcacatt gtaaatgaaa tagaaaataa
     3121 atttgatcaa atattacaag aaccattaaa tccattaaat tatgatactg tcagtggatt
     3181 agctgggata gggagatatt tgctaaatag agtagatgag aatgaattta atgttaaagc
     3241 attaaaaagc atattagtat actttaaaga tattcaatat tctaaaaata gctgggtagt
     3301 cccacaagaa agtcaatttt tagagtctga taaaaattat tttactgaag gtaatatcaa
     3361 tcttggcctt gcacatggag tgctaggacc gatgtcttta tttgcattat gcgtgattaa
     3421 aggaattacg attgaaaatc atcagcacat attaaaagac atgtacaaat ttatcatgga
     3481 cgaaaaattt tgtaaccacg aaagatggtt gcagcgttac gatttaattt ctgaacgtaa
     3541 tcatttcaat tttattcgga atggttggtg ttatggcaat acgggtgtaa tgacgacgtt
     3601 gtttttaatc ggccaagcat tacaagatga tgaaataatt aaaatgtcta aaaaagtgat
     3661 gctacaagta gtaaatgata aagatgaaaa tttaataagt ccaactattt gtcatggatt
     3721 gtcatcacaa atattaatgt taacaattat gaatttgaat tttgaattaa atgaagtgtc
     3781 tgattatatc actgtattaa taaataaact gatttctcat tataaggaag attatctggt
     3841 gaattttata gacattaatg aaaataagca agatgtattt aaaagtagga aagttggcct
     3901 tttagaaggt gaattagggg tctatctaac attgatgaca ctgaaaaata caaaaattct
     3961 caatgaaaaa aattggacca atgcgttttt aattagctga atggaggcgt atatcatggg
     4021 agaaaatgta ttgatttgtt tgtgtggctc agttaatagt at
//
LOCUS       DAMQYT010000205         4015 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMQYT010000205 DAMQYT010000000
VERSION     DAMQYT010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.209"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(23..580)
                     /gene="efp"
                     /locus_tag="PGA33_002381"
     CDS             complement(23..580)
                     /gene="efp"
                     /locus_tag="PGA33_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDG6474172.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
     gene            complement(606..1667)
                     /locus_tag="PGA33_002382"
     CDS             complement(606..1667)
                     /locus_tag="PGA33_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDG6474173.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDADEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            1772..2353
                     /locus_tag="PGA33_002383"
     CDS             1772..2353
                     /locus_tag="PGA33_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474174.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            2367..2585
                     /locus_tag="PGA33_002384"
     CDS             2367..2585
                     /locus_tag="PGA33_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474175.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLISFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(2649..3479)
                     /locus_tag="PGA33_002385"
     CDS             complement(2649..3479)
                     /locus_tag="PGA33_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDG6474176.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIEFKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            3637..>4015
                     /locus_tag="PGA33_002386"
     CDS             3637..>4015
                     /locus_tag="PGA33_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDG6474177.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKS"
BASE COUNT         1346 a          702 c          569 g         1398 t
ORIGIN      
        1 ctatttgtta acaaattaga gattatcctc ttgaaatgta gcttccatca ccagtgttga
       61 taattaaaac gtcaccttcg tttacaaata aaggtacatt taatgtataa ccagtttcaa
      121 cagttgccga tttagtggca ccagttgcag tatcaccttt aataccaggt tctgtttcag
      181 ttactgttaa ttcaacagtt ttaggtaatt caacaccgat agtttcacct tcgtatgttt
      241 gaatttgtac ttccatacct tcttttaagt aattcaattc ttcttttaag taatcacttg
      301 aaagttctgt ttgttcaaag ctttcattat ccataaatac atgattatct ccgtcagcat
      361 ataaatattg catgcgacga ttttcaatca ttgctggttc aactttttca ccagctctaa
      421 acgttttctc ttgaattgca ccagttctta aattacgtaa ttttgaacga acgaatgctg
      481 aacctttacc aggctttaca tgttggaagt ctataacttt ccaaatagcg ttatcaacag
      541 aaattgttaa acctgtttta aaatcattaa ccgaaatcat tcagtttcct cctcatttta
      601 cacgcttatg ttaaaactat aaggtctttt gtgcatttag taaagacttg acaaccattt
      661 tctgtaatta aaatatcatc ttctattctt ataccgccca aaccttctat ataaacacca
      721 ggttctactg taacacagtt gttaacttga agtttatctt gtatcgtacg agccagcatt
      781 ggcccttcat ggatttctaa accaatacca tgtcctagtg aatgtccaaa ttcttttcca
      841 tacccttttg actctaaata gtttcttgaa atggcatcag cttctgcacc agtcatgcca
      901 ggtctaatct cattaattgc tttcatttga gattcaagta ctatttgata tatttctttc
      961 agtttaggat ctggttctcc aatagcaaat gttctagtaa tatctgaaca atagccgtta
     1021 taatacgcgc caaaatctaa tgtaatcatg tcgccttttt caataatttt atcacttgca
     1081 acaccatgtg gtaatgcacc tctatgacca gatgctacaa tcgtatcgaa tgatggtcca
     1141 tctgctccta attctagcat tttgctttct aatattgcct ttaattcttt ttcagtcatg
     1201 cctgctttta caacagttaa aatatattca tatgtttcat caacaatatt agctgctttt
     1261 tgaattaaag caatttcgtc agcatctttg acgtctctaa ttttatctac agtattagaa
     1321 atgcttatta atgatatacg gcttttattt aattcaaggt atgtatcata acttacatga
     1381 tgcccctcaa aacctacatt ttcaaaattt tcttggtgta gcaattcttt aatctcacca
     1441 ataatagtag atttacgatt aataatttca taatttggcg cctgcttagt tgcttgatca
     1501 atatatctaa agtctgttat caaatattgt ttatctttag atatgataag tgctccactg
     1561 gtaccagtaa aacctgataa atatcttcta ttgtaatccg aaagaatgat aatcgcatct
     1621 aaatgttttt gttctaaaat acgatgcact tgtgttattc tgctcatctt attacctcct
     1681 agtgaaacgt ttgtgcattt caactttata cattaaaata atatcataat aaggataaaa
     1741 aataatagat attgatttta gggagatagt aatgaaaaaa ttggtttcaa ttgttggcgc
     1801 aacattattg ttagctggat gtggatcaca aaatttagca ccattagaag aaaaaacaac
     1861 agatttaaga gaagataatc atcaactcaa actagatatt caagaactta atcaacaaat
     1921 tagtgattct aaatctaaaa ttaaagggct tgaaaaggat aaagaaaaca gtaaaaaaac
     1981 tgcatctaat aatacgaaaa ttaaattgat gaatgttaca tcaacatact acgacaaagt
     2041 tgctaaagct ttgaaatcct ataacgatat tgagaaagat gtaagtaaaa acaaaggcga
     2101 taagaatgtt caatcgaaat taaatcaaat ttctaatgat attcaaagtg ctcacacttc
     2161 atacaaagat gctatcgatg gtttatcact tagtgatgat gataaaaaaa cgtctaaaaa
     2221 tatcgataaa ttaaactctg atttgaatca tgcatttgat gatattaaaa atggctatca
     2281 aaataaagat aaaaaacaac ttacaaaagg acaacaagcg ttgtcaaaat taaacttaaa
     2341 tgcaaaatca tgataggagt cttttaatgc gtaatataat attttatctt gtacttatta
     2401 ttgctgcgat tggattagta atgaatctag atgcctttat tttttcaatc gtcagaatgt
     2461 taatcagctt tgctgtaata gctggtatta tttatctgat ttattatttc ttcatcttaa
     2521 ctgaagacca acgcaaatat cgcaaagcaa tgcgtaagta taaaagaaat caaagaagaa
     2581 aatagataaa aaaacggaag cacttgtagg taaaatagtc tacgtgcttc cattttttat
     2641 tctaaaaact actttctaaa catccattca tctgaacgat atttttcagt taattcttcc
     2701 acttctgcca attgagcttc tgttaattca agtggcttaa attctatatt taaacctttc
     2761 ttaaaacctt tctcgaaagc ttcttccatt tgactaatag taatgtgttc atctgaaata
     2821 tcattgatgg caactgcttt ttcaacgaat gcctctttca tttttaattt taatctttca
     2881 tttttataaa taaacatatc aaacagttca tcaatatcaa tatcttgtaa aatcgaaccg
     2941 tgttggagga ttacgccctt ttgtctcgtt tgagcactcc cagcaatctt acggccttca
     3001 acaactagct cataccaact tggtgcatca aaacacactg aacttcgagg ttgttttaat
     3061 ttttgacgct cttcaggcgt tttaggtacc gcaaaataag tatcaaatcc taagttttta
     3121 aatccttcta ataatccttg tgaaatcact ctgtacgctt ctgtaactgt agaaggcata
     3181 ttcggatgcg attcaggcac aatcacactg taagttaact ctttatcatg tagcacccca
     3241 cggccaccag tttgacgcct tacgagacca aaacctttct ctttaacctt atcaatatca
     3301 atttcttttt gtagcctttg gaaataccct attgataatg ttgcaggatt ccatgtgtaa
     3361 aaacgtataa ctggatcaat ttcacctcta gagacaaaat ttaataacgc ttcatccatt
     3421 gccatattat aatatgggtc tttacttcct gtattaataa aattccaagt ttcagtcata
     3481 ttcaagaact cctatcagca aaatgtataa tatttgattc gctaattaat caatttaact
     3541 aaatgaataa taattgcaat tctttagtga aatattttga taatttgacc taacagtctt
     3601 ataattatat tatcgtttaa ttagggagga tgcaagatga gtgctagttt gtacatcgca
     3661 ataattttag ttatagcaat tattgcttat atgattgttc aacaaattct taacaagcga
     3721 gctgttaaag aattagatca aaatgaattc cataatggga ttagaaaagc tcaagtcatc
     3781 gatgttagag agaaagttga ctatgactac ggtcacatta atgggtctcg caatattcct
     3841 atgacaatgt tcaggcaacg attccaagga ttaagaaaag atcaaccggt atacttatgt
     3901 gatgccaatg ggattgctag ctatagagcc gctcgtattt tgaaaaagaa tggatataca
     3961 gatatctata tgttaaaagg cggctataaa aaatggactg gaaaaataaa gtcta
//
LOCUS       DAMQYT010000206         3965 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMQYT010000206 DAMQYT010000000
VERSION     DAMQYT010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.210"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(381..1658)
                     /locus_tag="PGA33_002387"
     CDS             complement(381..1658)
                     /locus_tag="PGA33_002387"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDG6474178.1"
                     /translation="MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAKVAEALKKEPRANTQAAEHILQEIRQQ"
     gene            1912..3078
                     /locus_tag="PGA33_002388"
     CDS             1912..3078
                     /locus_tag="PGA33_002388"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDG6474179.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(3248..3769)
                     /locus_tag="PGA33_002389"
     CDS             complement(3248..3769)
                     /locus_tag="PGA33_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDG6474180.1"
                     /translation="MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVG
                     KLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
BASE COUNT         1258 a          782 c          640 g         1285 t
ORIGIN      
        1 taagaaatga gaaattaaat tcttatatga tgaataaatg atgactgcag aagaaattgc
       61 taaaaaagga gtggagtgag gcattctact gttcatattg ttaacaaata tcaaaatgat
      121 aggaacttga agaatctaag actgttaaaa tcgagacaat aagaaactcg ttccaaattt
      181 gaatcctata atgaattcgt ctcatgaaga agttatttac ttttcattag actgatttac
      241 taatcaaaaa acttctatca cgaacgcttc aatttcctgt atgcgtgtga tagaagtttt
      301 tatttttgtt catattaatt tatctaagcg ctacgatgga ctgacttaaa cttttcttta
      361 aaatcgctat tcgccattta ctattgttgt ctaatttctt gtaaaatatg ttccgctgct
      421 tgtgtatttg cacggggttc ttttttcaaa gcttcagcta ctttagcaat ttcatcacct
      481 tttgccccta caacgatagc taatgattta tattgtaaac tcatatgacc ttgttgaata
      541 ccttctgaca caagcgcgcg acatgctgca aagttttgcg ctaaaccaac ggcagcaact
      601 acatgaccta attcttgtgc tgactctaca tttagtagct ctaatgaagc tttagcaatt
      661 ggtaatactt ttgtaccacc gccaacgatt gccaatgtca taggcacttc aattgtacca
      721 atcaatcgtt gacgatcttg atcgtaacgc catgtagcaa taccacgata ctgtccgtca
      781 cgactcgcgt atgcatgcgc acttgcttct gcaccacgcg tatcatttcc tgttgctaaa
      841 acaacagcat gtatgccatt cataacacct ttattatgtg ttgctgcacg atgaatatct
      901 acttgggcca atacagaagc acgttccatt cgtttggcaa cctcttctcc agttctctcg
      961 ccccttgcta aatctttaac atcaatttcg ccttgaactt taacaacgga cgctgttgca
     1021 tgattggata aaatactcat taaaatgtcg ctttgcggaa attcattttt taaaaatgca
     1081 gttatggcct ctaaaatcgt attaagcata ttagcgccca tagcatcttt cgtatcaaca
     1141 aatactttta aagatagtaa ctgttgctca ggaaatgtat caatcgctat acgttggtaa
     1201 ccaccaccac gcgctttaat agaaggatat gcctcatccg caattttatg aatttgcttt
     1261 tctaaagctt taatgtctgc tgataatttt tcagtatcgt caacgccatc aaagacgatt
     1321 tgacctatca taatacgttc agaagatacc gttttaaatc cgccagtctg attcactagc
     1381 tttgcaccat aactagctgc agcgacaact gaaggctctt ccaccatcat aggtacaaca
     1441 tatgccttat cgtccacaat gatattcggt aataatccaa cgggtaatgc accttgcgcg
     1501 atgacatttt caattaaact attggctact tcttcatcga ttaatggatg attcagtaaa
     1561 atgtcgaatt gttcttctga taaccattgc ttatcaacca attgttgtaa cttttcttta
     1621 cgagataaat gtcgaaaatt cttatctaaa ttttgcatgg acgtactcct tttacttcac
     1681 ataattttta acatttcaat cactactatt tttaccacaa aataacgtca ttcgtcttaa
     1741 aattcaattg aataattgtc gttttgactt taaaataaaa caaggtaaat taaaacgctt
     1801 acaagaaacg acaaatcatt tttaaattta gtatatttct ttgtataaaa ttagcatatt
     1861 ctgatatgat acaagtgttg cttttataaa tttgaaagga tgtaaaacct tatgacaata
     1921 ggtatcgata aaataaactt ttacgttcca aagtactatg tagacatggc taaattagca
     1981 gaagcacgcc aagtagaccc aaacaaattt ttaattggaa ttggtcaaac tgaaatggct
     2041 gttagtcctg taaaccaaga catcgtttca atgggcgcta acgctgctaa ggacattata
     2101 acagacgaag acaaaaagaa aattggtatg gtaattgtgg caactgaatc agcagttgat
     2161 gctgctaaag cagccgctgt tcaaattcac aacttattag gtattcaacc ttttgcacgc
     2221 tgctttgaaa tgaaagaagc ttgttatgct gcaacaccag caattcaatt agctaaagat
     2281 tatttagcaa ctagaccgaa tgaaaaagta ttagttattg ctacagatac agcacgttat
     2341 ggattgaatt caggcggcga gccaacacaa ggtgctggcg cagttgcgat ggttattgca
     2401 cataatccaa gcattttggc attaaatgaa gatgctgttg cttacactga agacgtttat
     2461 gatttctggc gtccaactgg acataaatat ccattagttg atggtgcatt atctaaagat
     2521 gcttatatcc gctcattcca acaaagctgg aatgaatacg caaaacgtca aggtaagtcg
     2581 ctagctgact tcgcatctct atgcttccat gttccattta caaaaatggg taaaaaggca
     2641 ttagagtcaa tcattgataa cgctgatgaa acaactcaag agcgtttacg ttcaggatat
     2701 gaagatgctg tagattataa ccgttatgtc ggtaatattt atactggatc attatattta
     2761 agcctaatat cattacttga aaatcgagat ttacaagctg gtgaaacaat cggtttattc
     2821 agttatggct caggttcagt tggtgaattt tatagtgcga cattagttga aggctacaaa
     2881 gatcatttag atcaagctgc acataaagca ttattaaata accgtactga agtatctgtt
     2941 gatgcatatg aaacattctt caaacgtttt gatgacgttg aatttgacga agaacaagat
     3001 gctgttcatg aagatcgtca tattttctac ttatcaaata ttgaaaataa cgttcgtgaa
     3061 tatcacagac cagagtagtc ggtgtattta aaacacatat aataaaacct aaaagcagca
     3121 gtaagaccac ttctaattga aatcgtctta ctgctgttct ctatttataa cacttcgtat
     3181 tgaatgaatt cattatgcct atttgacaca ttattgaagt tttcctaatg cctggatcct
     3241 ttatacgtta cggcttcgtg ctatgttttg gtacataaag ctttgacata tcgatattct
     3301 ccaactctaa cagcttaatt ttattattaa tcgttccacc gaaccctgtt aagctacccg
     3361 ttttaccgac aacacgatga catggcacga taatagataa tggattactt ccgactgcac
     3421 ctccaaccgc ttgggctgac atttttggct tgttaagcag cttgcctact tttttggcaa
     3481 tagcaccata cgttgttaga gtcccataag gaacctgtct taattcattc caaacacact
     3541 gttgaaaatg actacctgtt ggctttaaag gtattgtgat ttcaggattg tcacctttaa
     3601 aatacgcgtc taaccactgt gtcgcctctc taaatatcgc taaagacgta ttttcttccc
     3661 tagtaccatc accttgttga ttttcaaaca aaacagcggt cagacttacc ccatcactca
     3721 aaagttccaa tcgtcctaca ggcgaatcat agtaactctt atactccata aaaatccccc
     3781 ctttttctaa tcatacgcct taattgttaa attagaaaat attcatgttc acatttttta
     3841 aaaataaata agtcgtcaat tacgtatata aacacgtaat accagctatc actttgctgc
     3901 aatatacagt tacatatctt actacacgtg ctaacctctt actttgtaaa ccaaatctta
     3961 aatta
//
LOCUS       DAMQYT010000207         3962 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMQYT010000207 DAMQYT010000000
VERSION     DAMQYT010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.211"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            826..2889
                     /locus_tag="PGA33_002390"
     CDS             826..2889
                     /locus_tag="PGA33_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDG6474181.1"
                     /translation="MNNNGEEHNHQNHMNHSNQMHHDNHASHDHHSGHAHHHGNFKVK
                     FFVSLIFAIPIILLSPLMGVNLPFQFTFPGSEWVVLILSTILFFYGGKPFLSGGKDEI
                     ATKKPGMMTLVALGISVAYIYSLYAFYMNNFSSATGHTMDFFWELATLILIMLLGHWI
                     EMNAVGNAGDALKKMAELLPNSAIKVMDNGQREEVKISDIMTDDIVEVKAGESIPTDG
                     IIVQGQTSIDESLVTGESKKVQKNQNDNVIGGSINGSGTIQVKVTAVGEDGYLSQVMG
                     LVNQAQNDKSSAELLSDKVAGYLFYFAVSVGVISFIVWMLIQNDVDFALERLVTVLVI
                     ACPHALGLAIPLVTARSTSIGAHNGLIIKNRESVEIAQHIDYVMMDKTGTLTEGNFSV
                     NHYESFKNDLSNDTILSLFASLESQSNHPLAISIVDFAKSKNVSFTNPQDVNNIPGVG
                     LEGLIDNKTYKITNVSYLDKHKLNYDDDLFTKLAQQGNSISYLIEDQQVIGMIAQGDQ
                     IKESSKQMVADLLSRNITPVMLTGDNNEVAHAVAKELGISDVHAQLMPEDKESIIKDY
                     QSDGNKVMMVGDGINDAPSLIRADIGIAIGAGTDVAVDSGDIILVKSNPSDIIHFLTL
                     SNNTMRKMVQNLWWGAGYNIVAVPLAAGILAFIGLILSPAIGAILMSLSTIIVAINAF
                     TLKLK"
     gene            2907..3452
                     /locus_tag="PGA33_002391"
     CDS             2907..3452
                     /locus_tag="PGA33_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1541 domain-containing protein"
                     /protein_id="HDG6474182.1"
                     /translation="MIKKLFFMILGSLLILSACSNNDEKDKDTNDQKSESHMKHNDES
                     KVPEDMKSTNEGEFKVGDKVTITAGHMPGMKGAEATVKGAYKTYAYVVSYKPTNGNEK
                     VNNHKWVVNEEIKDAPKDGFSKGDTVKLEASHMSGMKGATANIDNVKKTTVYVVDYKS
                     KDNGKIIKNHKWMTGNELKAR"
BASE COUNT         1448 a          527 c          702 g         1285 t
ORIGIN      
        1 attattttta tttataacta tatattttat atgaaaaata taagttataa taataagttt
       61 aatattgcct cgtggttctg agcttgaact tatctctaaa tcattttgag ctacttatct
      121 atcaattcat atattctata acaatatttg tgacatcacg tgctatttca tgaagtgatt
      181 ttacgatatc accttcttta gaaaaaatat atttcagtag gcaccgacgt atacagaatc
      241 atttgagtat taaaaataaa aactagaaaa agaaaccgca tcattaactg atacgcagaa
      301 tcatattata aataaaacta aaaatgaggt tgtatataac tcactctgaa attgattgaa
      361 tatatagtat ctttaataaa atgcagctat tgtggcgtag aatttgagaa tcaaaaaatg
      421 attaatatag tttgaagaga ctgagcataa atactagaaa aatggccagt aaatgagttt
      481 actataaact catttactgg cttctctaat aattatcaag acaatttgcg tttctaggca
      541 tactttgaaa tgcgctattt tctttaagaa tattaatata tgacttgtgt tggtaatgat
      601 aaccttggtt agagtgtatt gtcatgtgat agttaagatt agatctttta attaaaacat
      661 tgttttaaat atcgatgaca aggtctaatg taggacgtgt agatataagg ttcggaatta
      721 tataaatcca taaaaggaga taaatcgaaa acctttatac cctgggtggg tatatgttat
      781 agtataagta gctttactat aacattttca ttaggagggg ttaatttgaa taataatggt
      841 gaagagcata atcatcaaaa tcacatgaat cattccaatc aaatgcatca tgataaccat
      901 gcctcacatg atcatcatag tggccatgca catcatcatg gaaattttaa agttaagttt
      961 tttgtttcat taatttttgc aatacctatc attcttttat cgccactgat gggtgttaac
     1021 ttaccttttc aattcacatt tccaggttct gaatgggtag tgttaatatt aagtacaatt
     1081 ttattctttt atggtggtaa accgttcttg tctggtggta aagatgaaat tgctacaaaa
     1141 aaaccaggca tgatgacctt agttgcccta ggtatttcag tagcttatat ttatagcttg
     1201 tatgcttttt atatgaataa ctttagtagt gcaactggtc atacaatgga ctttttttgg
     1261 gaattagcaa ccttaatttt aattatgcta ttaggacatt ggatagaaat gaatgctgtc
     1321 ggaaatgctg gagatgcttt aaagaaaatg gcagaactgt tacctaatag tgctattaaa
     1381 gttatggata atggccaacg cgaagaagtt aaaatatcag acatcatgac tgatgatatc
     1441 gtcgaagtaa aagccggaga aagcattcca acagatggta ttatcgttca aggacaaaca
     1501 tctatagatg aatccctagt cactggagaa tctaaaaaag tacaaaaaaa tcaaaatgac
     1561 aacgtcatcg ggggttctat taatgggtct ggaacaatac aagtcaaggt tacagctgtg
     1621 ggagaagatg gatatctttc tcaagttatg ggacttgtta atcaagcaca aaatgataaa
     1681 tctagtgctg aattgttatc tgataaagta gcgggttatt tattctactt tgctgtaagt
     1741 gttggcgtga tttcttttat tgtctggatg ctcattcaaa atgatgttga ttttgcatta
     1801 gaacgtcttg taactgtgtt agtcattgct tgtccacatg ctttaggctt ggcaatacct
     1861 ttagtcactg cacgttctac ttcaattggt gcacataatg gtttaattat taaaaataga
     1921 gagtctgtag aaatagctca acatatcgat tatgtaatga tggacaaaac tggtacttta
     1981 actgagggta acttttctgt gaatcattat gagagcttta aaaatgattt gagtaatgat
     2041 acaatattaa gccttttcgc ctcattagaa agtcaatcta atcacccatt agctataagt
     2101 attgttgatt ttgcgaaaag taaaaatgtt tcatttacta acccacaaga cgttaataat
     2161 attccaggtg tcggattaga aggtctaatt gataataaaa catataaaat aacaaatgtc
     2221 tcttatcttg ataaacataa acttaattat gacgatgact tgtttactaa attagctcaa
     2281 caaggtaatt caatcagtta tttaattgag gatcaacaag tcattggcat gattgctcaa
     2341 ggagatcaaa ttaaagaaag ctcaaaacaa atggtagctg atttactatc aagaaatatt
     2401 acaccagtca tgcttacagg tgacaataat gaagtggcac acgctgtcgc aaaagaatta
     2461 ggtattagtg atgtccacgc acaactcatg ccagaagata aggaaagcat tataaaagat
     2521 tatcaaagtg acggtaataa agtcatgatg gtcggagacg gtatcaacga tgcgccgagt
     2581 cttataagag cggatattgg tatagcaatt ggtgcaggta cagatgttgc agtggattca
     2641 ggtgatatca tacttgttaa aagtaatcca tcagatatca ttcatttctt gaccctttca
     2701 aataatacta tgagaaaaat ggtgcaaaac ttatggtggg gtgcaggtta taatattgtt
     2761 gctgtacctt tagcagctgg tattttagca tttattggct tgattttatc acctgcaata
     2821 ggtgctattt taatgtcttt aagtacaatt atagttgcaa ttaatgcctt tacattaaaa
     2881 ttaaaataaa agataggagt tttattatga ttaaaaaatt attttttatg atattaggat
     2941 cattactaat attatcagct tgctccaata atgatgaaaa agataaagac actaatgacc
     3001 aaaaaagtga gagccatatg aagcataatg atgaaagtaa agttccagaa gatatgaaat
     3061 cgactaatga gggtgaattt aaagtgggag ataaagtaac gattacagca gggcatatgc
     3121 caggtatgaa aggtgcagaa gctactgtaa aaggtgcgta taaaacatat gcctatgttg
     3181 taagttataa acccacaaat ggaaatgaaa aagtaaacaa tcataaatgg gtcgtaaacg
     3241 aagagattaa agatgcacct aaagatggat ttagtaaggg cgatactgtt aaattagaag
     3301 caagtcatat gtctggtatg aaaggtgcta cagccaatat agataacgtg aaaaagacta
     3361 ctgtttacgt agttgattac aaatccaaag ataatggtaa aatcattaaa aatcataaat
     3421 ggatgacagg aaatgagctg aaagcacgat aaaaatctag ttctaaattg agaaataaat
     3481 agatataaaa atatcctcct taatcaataa tttaaataac ttattattgt taaggaggat
     3541 attttttagt gtgtaaatta aaaagaattt tagaagaata acatttatca aaaaactgtt
     3601 cattacctta ttaattgaaa ttatataatt aaaaaccgca tcattaaccg atacgcagag
     3661 gcgtatcata agtaaaacta aaaaattctg tatgaggaga taataatttg gagggtgtta
     3721 aatggtggac attaaatcca cgttcattca atatataaga tatatcacga taattgcgca
     3781 tataacttaa gtagtagcta acagttgaaa ttaggcccta tcaaattggt ttatatctaa
     3841 aatgattaat atagaatgct tctttttgtc cttattaaat tataaaagta actttgcaat
     3901 agaaacagtt atttcataat caacagtcat tgacgtagct aagtaatgat aaataatcat
     3961 aa
//
LOCUS       DAMQYT010000208         3949 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMQYT010000208 DAMQYT010000000
VERSION     DAMQYT010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.212"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(697..2880)
                     /locus_tag="PGA33_002392"
     CDS             complement(697..2880)
                     /locus_tag="PGA33_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDG6474183.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(2877..3554)
                     /gene="recU"
                     /locus_tag="PGA33_002393"
     CDS             complement(2877..3554)
                     /gene="recU"
                     /locus_tag="PGA33_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDG6474184.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNTFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEVFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
BASE COUNT         1260 a          650 c          621 g         1418 t
ORIGIN      
        1 aagaaagata tgattatatg attgaacgta aatatcattt tatgggctat cgacaaatga
       61 agacctttaa aactgaattg attaaaatga atgctagtta tcaaacaagg ttaaagaata
      121 agcaggtata gacaagattg atcatttcct aatgttgaaa atatataaaa ctaattcatg
      181 cacacatatt tataattatg ttaggtgtcg tgtcattaca acgtcttaat ataaaaactg
      241 tccatctaca tcataatgga tatcttgaga tatgctttaa gatatctatt tcgaatcgta
      301 ggtggatttc ttatatctat gattgtgaaa atatgttgca aacatgttga tatttcactg
      361 attaacgtac acagggaatt aataaatata tcaatttaaa acagcgtcta agatgactat
      421 gagaaaatat aatcataatt ttaaaaatgg gaacaaccac ttattcaaat tcaataattt
      481 tacatagcta aagatgtgaa tatgaaaaac tatttagatg tttcaaaatg tatgtgaatg
      541 actgatttta cggtttctac taatatagca atagcgtatg aatacttata aaaaaagcag
      601 gaagttttac cttcccacca taaaagatga agaacctaaa aaaatcctcg gctacaccat
      661 aaatatggta taactgagga ttttatgttg agtggattag ttgaatatac ctgttaatcc
      721 accgctgttt ctagattgtt gagatctagt attgttattt gattgtgcag taccactgct
      781 gtttgctgat gtgtcactac caccgtgtgt actacgattt gtagtgttgt tatcttgact
      841 accagaaaca ttgattgatg ggatactacc acttactgag ctaggacgtt taaagtcttc
      901 gccatctcta gatgaaattt ttgacatcac attttcatat aagaactgtg gatattcttg
      961 ttggctatgt cccacaaatg agttttcacc atattgttta actttactga agcccatcca
     1021 cactgacata gtgtattgag gtgtaaagcc gttaatccac acgtctttcg ctgcattatc
     1081 aggtaaatta tattgtgaat aagtttcagc accgtaagta ccagtacctg tcttagcacc
     1141 catatttact ccagatacac catggccata tgcagaacca tatggtttaa atgtaccttt
     1201 tagcatctca gctaacatgt atgcagtgta atcactcatc gctttatggc tagtatgatc
     1261 gtattcgatt gtttcaccat cacgagtaac tactttttga attgaatgcg cgttgttata
     1321 agtaccaccg ttagcgattg cagcaaatgc tgatgctaat tgtgttggtg agaattctga
     1381 agcagaacca ccaagtactt cagatggacc aatatcgcct tcgtagttta agccaagttt
     1441 ggcagcgaat ttcttaggtg catcattacc agcattttgc ttaactgatt gccaagcttt
     1501 taaagctggg atattgaaac tttgtcgtaa agcatcataa atagatacag taccgtgact
     1561 cttcgtatca tagtttctaa atgtagaacc atcaacttga tatgaagatt catcttgaat
     1621 cgcatggttt gttgcccatt tcatattttc aatggcaggt ccatacgcta agaaaggttt
     1681 taaagatgaa ccagtagggt gaggatctgt tgcttggttt ctgttaacga cgtctttgaa
     1741 atcacgtcca ccagatatag caactaaacc accagtttta ctatcaagaa ttgttgcacc
     1801 aacttgttgg tctttattct tgtagaagct accattatca acatcatttt gtaatgtttt
     1861 ttgaacatct ttatccatgt ttgtataaat tttaatacca ctttgtaata cattacctaa
     1921 attttcatct ttgaatgctt tattattcat taattcagat tttacaaagt taacgtatga
     1981 attatactca gaatcttgat ttgtatcaat gttttgacgt tcttcaggag tacgatttac
     2041 taagttcgct tttaaatcga ttttcttagc atcttcccac tgtttatctg taatgcgttt
     2101 atgataatgc attaagtata aaacagtgtt tttacgatct tcagcagctt ttggatgatc
     2161 ataaatatta tagttgtttg gaacctgagg taaaccggct aaataagctt cttccgctaa
     2221 gtttaaatct tttaaatctt tattaaagta atacttagca gcagctttaa tacctgttac
     2281 gccatcagaa tagtaaattt tgtttagata tacttggaag atatcatctt tactatactc
     2341 ttgttctaaa cgatatgata agtatgcttc ttgagcttta cgtccaatag atttatgttg
     2401 tgataaaaat gcatctttaa caacttgttg tgttaatgtt gaggcacctt cagaaccaaa
     2461 tccaccagtc aagttcttac caattgcacc gaataaacgt ttataatcaa gtgcgccatg
     2521 ttcgtagaaa cgattgtctt cagttgcaag tactgcgtct ttcattgatt tcggcacgtc
     2581 ttttaaattt acatgctcat gtctttggcc attatctaat gttttaacaa gttctccgtt
     2641 cttgtcatat atctttgcag gaatcggatc ttgtaattta gcttcggtaa aagcaggtgc
     2701 tttccaagca taataagcaa acaataagat acctagtaaa agaacaacga aaaatgcaat
     2761 aatcatgaag ccaataatct taataatcgt tctcttcaca tttctattct tttttgagtt
     2821 ggatttccca ccattattac cgtttttctt aggctgagaa gatcctttgt tttccgtcat
     2881 acgcggtcct cactttcatc taatatcaac ttatcaactg cttttagata gtctaatctt
     2941 ggttgatact gataaggaat atggtaacca ttttttcgta tttcatcaac tgttatagac
     3001 tttttaatat tatctttata tctcttccaa aatacttcga attttgaata gggtaaaaga
     3061 taaacttcat ctagcgtttt aaaacgaatc attaaaaaca caataccttt ttgttgatat
     3121 gcatttttca tatgttcgac ttgatggtca tgaatattat ttaaaggaaa ggacgtcttg
     3181 tttttagttt cctttgcttc aaaatcaata taataacctt gataaacgcc gttgtaatca
     3241 gttgttgaag gtgtacgaaa ataagcttcg ttaatcacag ctttactccg cttaggatag
     3301 ttgacattaa ctatttgtac tggcgtaggc tttttgtgaa taactgcaat gtcgctttta
     3361 agataaaacg tatttgaatg ttcgatatct ttttcaagtg acataccacg tccaccatac
     3421 tcaatattac taaaggcagc ggtctttttc cctccgtcta tagcactatt tttacgatat
     3481 ggtttaccat ttggataatt cataaatttc accacacaag tttggtagta aacgttgttt
     3541 attatacaca acattatagt gaaacaccaa acttaaatta gtataaatgc gaaagacaca
     3601 atacaccaaa gcttatttta acgtgatttc agcattaaca caataatatt ataatacatt
     3661 taccgttaat gttggaattt tacacacaat tttaattaat tgaaaagttt tgcttataaa
     3721 aatgaatact cattacaata tttttgaatt ttgttataaa agtcaaatca tgattagtaa
     3781 tttttatttt tgaggcaagt acaagtatgt gttgttaatc agcagaggtg tatttgattt
     3841 aaatgatttt attagattaa aagtattttc atcgtataac aacatacacg ataaaatttt
     3901 gtatttatat aggaagtttt tagatttact tgatttaaca gataacaca
//
LOCUS       DAMQYT010000209         3854 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMQYT010000209 DAMQYT010000000
VERSION     DAMQYT010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.213"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..426)
                     /locus_tag="PGA33_002394"
     CDS             complement(<1..426)
                     /locus_tag="PGA33_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDG6474185.1"
                     /translation="MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNL"
     gene            complement(398..1372)
                     /locus_tag="PGA33_002395"
     CDS             complement(398..1372)
                     /locus_tag="PGA33_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDG6474186.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMHKYI"
     gene            complement(1424..2047)
                     /locus_tag="PGA33_002396"
     CDS             complement(1424..2047)
                     /locus_tag="PGA33_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDG6474187.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            complement(2044..2373)
                     /locus_tag="PGA33_002397"
     CDS             complement(2044..2373)
                     /locus_tag="PGA33_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDG6474188.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            complement(2373..3359)
                     /locus_tag="PGA33_002398"
     CDS             complement(2373..3359)
                     /locus_tag="PGA33_002398"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDG6474189.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            complement(3356..3559)
                     /locus_tag="PGA33_002399"
     CDS             complement(3356..3559)
                     /locus_tag="PGA33_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDG6474190.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            complement(3540..>3854)
                     /locus_tag="PGA33_002400"
     CDS             complement(3540..>3854)
                     /locus_tag="PGA33_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474191.1"
                     /translation="DNAQNIVKQTINKNYADDQTYYLSGMIMATINSKSEGMTEWERG
                     LRMFPKSGLLNFELAIANRSLNDDEKALKYVRKALNADPKNTDYINLEKELTKSNESK
                     NK"
BASE COUNT         1219 a          712 c          516 g         1407 t
ORIGIN      
        1 gaggtttaat ataataatca tagcaataaa gatagaaact agtattaagg ggtattgcag
       61 tgtctttaac aatcgttgtt ctgactttct attcactttc atataattta cggtttcttc
      121 tagaacgtct ataatattgc caaatctttc tgccaaatat acttgcatga cgatagtatc
      181 gctataacct atcagtgata atatttgatt gcatggtgca ccatttgaaa tttcacttag
      241 aatggtggta cctaattgct tatttttata tgtcatttga agatttaaaa attgaaaact
      301 ttgatacaga gtgaaaccat atttcaacaa attacataaa tttgaaagta agtcgatttg
      361 ttgggcctta cttaattgtc gcttcttaga atgtagttta aatgtattta tgcattgtag
      421 tttcacaaat gacacctgct tttgtcatat catcaagttt atcttctaag ttcttaaatg
      481 atgatggtaa tgaatgatta tgggaaaaga aatatcggag ttgttgctga gatagaattt
      541 cacatactaa ttgtcgctgt tgcttaatag tagttacaag tcgttggttt ataattaagt
      601 tagttgcctg tatcaattct tgtacagaaa tgcccatttc taatagcctt aaaatagcac
      661 ctttacaatc agttgcatgc aatgtagtca gaacaaggtg accacttaaa ctagcctgta
      721 taacacactt ggcaacatct ttatctctga tttcacctat taaaataaca tcaggatcac
      781 atcttaaaat agctttaaac gaatttacat agtttatgcc agctttatca ttcacattaa
      841 tttggacgat accaggaatt tgcatctcta caggatcctc tatagaaatt acatttaaat
      901 tcaaggcttt attcgcgtat gagaccattt gatacattaa tgtactcttt cctgaaccag
      961 ttggtccact aaacaatagt aatccttgtt tcttattcat gaggtgttta aaatcattga
     1021 atttataagt tgatttctgt ggttgaaaaa attgaggtac aattctgata acacaacttt
     1081 cttggccaag tgacaatggt aaagttgata ttctcaaaaa atatatttta ttgaaaaggt
     1141 aactatatcg accgctctgt gcgacttgct gtgtagaaac atcaagccca gcttgaaact
     1201 tcatataaac taataacttt tgataaatgc tattcccaat ttgttcatac tgctccaagt
     1261 tatcattaat tctaaattta atacttactt catttttaac tggaataaaa tgtacatcac
     1321 tcgctttcat ttctatcgct ttattaatta tttcttgaaa tagaatcttc aaaaaaacac
     1381 ctcctacata taatcacgta ggaggtgttt ttattacttc aatttaaccg tgtaaaaatg
     1441 gatttaattg ttcatcatca accgtcgtat atggaccatg tccagggaat aaaggtaaat
     1501 cgccttctaa ttcaaatatt ttatcttgaa tagaatcaac tagcgtttca taatcacctt
     1561 tatataaatc tgtacgtccg attccattat taaataatgt atctccaaca actgcgaatt
     1621 catcgaacac atatgttaaa cttcctggtg aatgtccagg tgtgtgtaac acattaaact
     1681 taaatccttc tatttctgtg ctaccttcgt ttaacttttc aggagttacc ttacttgtaa
     1741 taattggtaa tccatattgc ttaaatttat ctgccccatt tttaacggga tcttttagaa
     1801 aatcaaactc tgcttcatgc atataaaccg ggacatcgaa tcgatcaact atatcatcga
     1861 ctgctccgat atgatcaaag tgtgcatgtg ttaataaaat agcttttaac ggtttattta
     1921 tttggtttaa ttttttaata attttttcac tttcacctga agggtcaatc agaataacag
     1981 ctttgtcatt ttcgatgaaa tacgtattag tatcaactaa gcctaaagtt aagcttgaaa
     2041 tcctcatagg ttttcaccac tattttctaa atgtttttgt actgatgtta gtttatcatt
     2101 catttttcta gatttgtctc gtcggtcatc aatacggata tttgtacaaa ctctacttaa
     2161 acctttatca aaaggtaatt catgtatcac ttgcacaact tctaatacat cgcttaattc
     2221 accttcaatt agagtattca ttggtgttaa ttgaaaatca attttaccca ttgctttata
     2281 ttcttgaagt tttttctgaa tatctgcaat atatttacta acactcggac cttccgttcc
     2341 aactggaata acaaccacat caacaatagc cattatttta ccccctcttt atctaataca
     2401 taggtcttga ttaatcctgc tgctcctgta atacctgcat cattacctaa tttcgcttgt
     2461 acaatttcag tttcaaattg agcaggtgca aatgttaaat tatgatattc tgttttaata
     2521 ttttcaatta aaataggtcc tgcagtagac attcctccac ctagaacgat atatttcgga
     2581 ttacttgtaa cactaataat actacataaa tatccaatat agtttgcaac cttttcagta
     2641 atgaaaatac agaattggtc accagctttt gccgcatcaa aaacagcttt tgctgtaacc
     2701 ttattttctt taatcaattc taatatagaa gatctaaacg tcaacttcgg atagtagaag
     2761 ttaactaagt taacaacgcc tgtcgctgaa gcaactgttt caatacatcc agaacgacca
     2821 caattacatt taaatcgttg atcgaagtct gctctaaaat gacctatttc tgcgccagag
     2881 ccattatgac catgtacgat ttcaccattt gaaataattc ctccacctag acctgtacca
     2941 agtgtgatgg caacaacatc atcggcacct tcaccagcac ctttgtgttt ctcccctaaa
     3001 gcagctatgt tagcatcatt atctacatac actggacaat caacgaattg ttcaaaaatc
     3061 tcacgtacat taactttttc tggccaatat aagtttactg ctccatttac tgtacctttt
     3121 tcaaagtcaa caggacctgg tacaccaata cctacgccaa gtacatttga aaaattataa
     3181 ttattttcat ttactttttc aacaaacgaa tcataaattc ctttcaaaag tgtatatcct
     3241 gtactatcag atgtatcagt gtgaatagac catttatgta attgttctaa ttcaggtgtg
     3301 aaaataccta atttacaagt cgtcccgcct acatcagctg ctaaaataat ttttgtcatt
     3361 tctgttcatt ccttctctga ttaattatca acgtacattt caaatattct tccttagata
     3421 acagttcata ttgatacaat gatgaaatct cctgttgaat catttcgtac atatcttctg
     3481 gatttttaaa atatattaga aatccgtaac ttttcaataa ctgttgtaca tcataaaagt
     3541 tatttatttt tcgactcatt tgatttagtc aactcttttt ctaagttaat ataatctgta
     3601 tttttagggt ctgcatttaa tgctttacgc acatatttta atgctttttc atcatcattt
     3661 aatgaacgat ttgctatcgc taactcaaaa tttaataaac ctgatttagg aaacattcta
     3721 agtcctcgct cccattctgt cataccttcg gacttagaat tgatagtagc cataatcata
     3781 ccacttaaat aatatgtttg atcatcggca taatttttat ttattgtctg cttcacaata
     3841 ttttgagcat tatc
//
LOCUS       DAMQYT010000210         3773 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMQYT010000210 DAMQYT010000000
VERSION     DAMQYT010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.214"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            71..2146
                     /gene="topA"
                     /locus_tag="PGA33_002401"
     CDS             71..2146
                     /gene="topA"
                     /locus_tag="PGA33_002401"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDG6474192.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKVEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            2302..3609
                     /gene="trmFO"
                     /locus_tag="PGA33_002402"
     CDS             2302..3609
                     /gene="trmFO"
                     /locus_tag="PGA33_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HDG6474193.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FTGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
BASE COUNT         1447 a          560 c          730 g         1036 t
ORIGIN      
        1 ataaaacgtt gacaaaagca aaattaagcg tttatcattt atctttagta attagattag
       61 cgagggggaa atgacattgg cagataattt agtcattgtt gaatcgcctg caaaagcaaa
      121 aaccattgaa aagtatttag gtaagaaata taaagttata gcttcaatgg gacacgtcag
      181 agacttacca agaagtcaaa tgggtgtcga cactgaagat aattacgaac caaaatatat
      241 aacaatacgc ggaaaaggtc ctgttgtaaa agaattgaaa aaacatgcaa aaaaagcgaa
      301 aaacgtcttt ctcgcaagtg accccgaccg tgaaggtgaa gcaattgctt ggcatttatc
      361 aaaaatttta gagcttgaag attctaaaga aaatcgcgtt gttttcaacg aaataactaa
      421 agacgctgtt aaagaaagtt ttaaaaatcc tagagaaatt gaaatgaact tagtcgatgc
      481 acaacaagcg cgtcgaatat tagatagatt agttggctat aacatctcgc cagttctttg
      541 gaaaaaagta aaaaaagggt tgtcagcggg tcgagttcaa tctgttgcac ttcgtttagt
      601 cattgaccgt gaaaatgaaa ttcgaaactt taaaccagaa gaatattgga ctattgaagg
      661 agaatttaga tacaaaaaat caaaattcaa tgctaaattc cttcattata aaaataaacc
      721 ttttaaacta aaaacgaaaa aagatgttga gaaaattaca gctgcattag atggagatca
      781 attcgaaatt acaaacgtga ctaaaaaaga aaaaacgcgt aatccagcaa acccatttac
      841 aacttctaca ttacaacaag aggcggcacg taaattaaac tttaaagcaa gaaaaacaat
      901 gatggtcgca caacaattat atgaaggtat agatttgaaa aaacaaggta cgattggttt
      961 aataacatat atgagaaccg attctacacg tatttcagat actgccaaag ttgaagcaaa
     1021 acagtatata actgataaat acggtgaatc ttacacttct aaacgtaaag catcagggaa
     1081 acaaggtgac caagatgccc atgaggctat tagaccttca agtactatgc gtacgccaga
     1141 tgatatgaag tcatttttga cgaaagacca ataccgatta tacaaattaa tttgggaacg
     1201 atttgttgct agtcaaatgg ctccagcaat acttgataca gtctcattag acataacaca
     1261 aggtgacatt aaatttagag cgaatggtca aacaatcaag tttaaaggat ttatgacact
     1321 ttatgtagaa actaaagatg atagtgatag cgaaaaggaa aataaactgc ctaaattaga
     1381 gcaaggtgat aaagtcacag caactcaaat tgaaccagct caacactata cacaaccacc
     1441 tccaagatat actgaggcga gattagtaaa aacactagaa gaattgaaaa ttgggcgacc
     1501 atcaacttat gcaccgacaa tagatacgat tcaaaagcgt aactatgtca aattagaaag
     1561 taagcgtttt gttcctactg agttgggaga aatagttcat gaacaagtga aagaatactt
     1621 cccagagatt attgatgtgg aattcacagt gaatatggaa acgttacttg ataagattgc
     1681 agaaggcgac attacatgga ggaaagtaat cgacggtttc tttagtagct ttaaacaaga
     1741 tgttgaacgt gctgaagaag agatggaaaa gattgaaatc aaagatgagc cagccggtga
     1801 agactgtgaa atttgtggtt ctcctatggt tataaaaatg ggacgctatg gtaagttcat
     1861 ggcttgctca aacttcccgg attgtcgtaa tacaaaagcg atagttaagt ctattggtgt
     1921 taaatgtcca aaatgtaatg atggtgacgt cgtagaaaga aaatctaaaa agaatcgtgt
     1981 cttttatgga tgttcgaaat atcctgaatg cgactttatc tcttgggata agccgattgg
     2041 aagagattgt ccaaaatgta accaatatct tgttgaaaat aaaaaaggca agacaacaca
     2101 agtaatatgt tcaaattgcg attataaaga ggcagcgcag aaataatatt tttatttcct
     2161 agatacattt taagattgtt aaatagaatc attagtgaat cttattttaa agatagtaaa
     2221 ggattaatct aaataagtgc ggataatata aacataacaa cataattaaa agacataaat
     2281 gacaataaaa ggagtataga aatgactcaa actgtaaatg taataggtgc tggtcttgcc
     2341 ggttcagaag cggcatatca attagctgaa agaggaatta aagttaatct aatagagatg
     2401 agacctgtta aacaaacacc agcgcaccat actgataaat ttgcggaact tgtatgttcc
     2461 aattcattac gcggaaatgc tttaactaat ggtgtgggtg ttttaaaaga agaaatgaga
     2521 agattgaatt ctataattat tgaagcggct gataaggcac gagttccagc tggtggtgca
     2581 ttagcagttg atagacacga tttttcaggt tatattactg aaacacttaa aaatcatgaa
     2641 aatatcacag ttattaatga agaaattaat gccattccag atggatacac aattatcgca
     2701 acaggaccac ttactacaga aacccttgcg caagaaatag tggacattac tggtaaagat
     2761 caactttatt tctatgatgc ggctgctcca attattgaaa aagaatctat tgatatggat
     2821 aaagtttact taaagtcccg ttatgataaa ggtgaagctg catatttaaa ctgtcctatg
     2881 actgaggatg aatttaatcg cttttatgat gcagtattag aagctgaagt tgcgcctgta
     2941 aattcatttg aaaaagaaaa atatttcgag ggttgtatgc cttttgaagt aatggcagaa
     3001 cgcggacgca agacattact atttggacca atgaaaccag taggattaga agatccaaag
     3061 actgggaaac gtccttatgc ggtggttcaa ttaagacaag atgacgctgc tggtacactc
     3121 tacaatattg ttggcttcca aacgcattta aaatggggag ctcaaaaaga agtcattaaa
     3181 ttaattccag gcttagaaaa tgttgatatt gttagatatg gtgtgatgca tagaaatacc
     3241 ttcattaatt caccggacgt attaaacgag aaatatgaat tgatttcaca accaaacata
     3301 cagtttacgg gacaaatgac tggtgttgaa ggttatgtag aaagcgcagc tagcggctta
     3361 gttgcaggta tcaatcttgc gcataaaata ttaggcaagg gtgaggtagt atttccgaga
     3421 gaaacaatga ttggaagtat ggcttactat atttctcatg ctaaaaacaa taagaatttc
     3481 caacctatga atgctaactt cgggttatta ccatctttag aaactagaat taaagataaa
     3541 aaagaacgct atgaagcaca agctaataga gctttggatt acttagaaaa tttcaaaaaa
     3601 actttataaa atagttagaa agactagata tgctattcat tcttaagtca tcaacgagta
     3661 agtaatgact ttctaaatgg aaaatactta tcctagtctt tttaattttg gaattgttac
     3721 gtatttctga caatttagaa ttcgcattca aaaaatatct aaataaataa cac
//
LOCUS       DAMQYT010000211         3676 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMQYT010000211 DAMQYT010000000
VERSION     DAMQYT010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.215"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            45..1571
                     /gene="spa"
                     /locus_tag="PGA33_002403"
     CDS             45..1571
                     /gene="spa"
                     /locus_tag="PGA33_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HDG6474194.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDNNKPGKEDNNKPGKEDNNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDNKKPGK
                     EDGNKPGKEDGNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIK
                     PGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL
                     "
     gene            complement(1900..3492)
                     /locus_tag="PGA33_002404"
     CDS             complement(1900..3492)
                     /locus_tag="PGA33_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDG6474195.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISVDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
BASE COUNT         1395 a          713 c          567 g         1001 t
ORIGIN      
        1 gttatattat gatgacttta caaatacata cagggggtat taatttgaaa aagaaaaaca
       61 tttattcaat tcgtaaacta ggtgtaggta ttgcatctgt aactttaggt acattactta
      121 tatctggtgg cgtaacacct gctgcaaatg ctgcgcaaca cgatgaagct caacaaaatg
      181 ctttttatca agtcttaaat atgcctaact taaatgctga tcaacgcaat ggttttatcc
      241 aaagccttaa agatgatcca agccaaagtg ctaacgtttt aggtgaagct caaaaactta
      301 atgactctca agctccaaaa gctgatgcgc aacaaaataa cttcaacaaa gatcaacaaa
      361 gcgccttcta tgaaatcttg aacatgccta acttaaacga agcgcaacgt aacggcttca
      421 ttcaaagtct taaagacgac ccaagccaaa gcactaacgt tttaggtgaa gctaaaaaat
      481 taaacgaatc tcaagcaccg aaagctgata acaatttcaa caaagaacaa caaaatgctt
      541 tctatgaaat cttgaatatg cctaacttaa acgaagaaca acgcaatggt ttcatccaaa
      601 gcttaaaaga tgacccaagc caaagtgcta acctattgtc agaagctaaa aagttaaatg
      661 aatctcaagc accgaaagcg gataacaaat tcaacaaaga acaacaaaat gctttctatg
      721 aaatcttaca tttacctaac ttaaacgaag aacaacgcaa tggtttcatc caaagcctaa
      781 aagatgaccc aagccaaagc gctaaccttt tagcagaagc taaaaagcta aatgatgctc
      841 aagcaccaaa agctgacaac aaattcaaca aagaacaaca aaatgctttc tatgaaattt
      901 tacatttacc taacttaact gaagaacaac gtaacggctt catccaaagc cttaaagacg
      961 atccttcagt gagcaaagaa attttagcag aagctaaaaa gctaaacgat gctcaagcac
     1021 caaaagagga agacaataac aagcctggca aagaagacaa taacaagcct ggcaaagaag
     1081 acaacaacaa gcctggtaaa gaagacaaca acaagcctgg caaagaagac ggcaacaagc
     1141 ctggtaaaga agacaacaaa aaacctggta aagaagatgg caacaagcct ggtaaagaag
     1201 acaacaaaaa acctggtaaa gaagacggca acaagcctgg caaagaagat ggcaacaaac
     1261 ctggtaaaga agatggtaac ggagtacatg tcgttaaacc tggtgataca gtaaatgaca
     1321 ttgcaaaagc aaacggcact actgctgaca aaattgctgc agataacaaa ttagctgata
     1381 aaaacatgat caaacctggt caagaacttg ttgttgataa gaagcaacca gcaaaccatg
     1441 cagatgctaa caaagctcaa gcattaccag aaactggtga agaaaatcca ttcatcggta
     1501 caactgtatt tggtggatta tcattagcct taggtgcagc gttattagct ggacgtcgtc
     1561 gcgaactata aaaacaaaca atacacaacg atagatatca ttttatccaa accaatttta
     1621 acttatatac gttgattaac acattcttat ttgaaatgat aagaatcatc taaatgcacg
     1681 agcaacatct tttgttgctc agtgcatttt ttattttact tacttttcta aacaacttct
     1741 gaaacgcctc aacactttct actctgatta catatatgac atttttaggc attaaaaaat
     1801 cgaactagac aagatgctca ttgcatttcg tactagttcg attcatgaat aattagattt
     1861 aaaatgtcat ttgaatccaa gtgacaacat tatttatatt tagaatatta acgttagtat
     1921 aaacgtccaa acacaaataa aagcaacaaa tataatactg tattttaacg tcatttttaa
     1981 taatgcagat tcttcaccaa cttttttaac agctgcagtc gcaatggcaa ttgattgtgg
     2041 tgaaataagt ttcgctgcta caccacctgc agtgttagct gccacaagta atgaaccgct
     2101 tgttgaaatt tgttgtgcca ctgtcgcttg aataggtgcg aataaagtat tgttatttac
     2161 aactgaacca gtcataaaca caccaatcca acctaatact ggagagaata atgggaaaat
     2221 tgctcccgct ttagcaatac cttgtccaat tgctacagtc aaaccaccgt atgtcataac
     2281 tttagcaata gctaggatag ctgaaattgt aaggatcggt aaccataatt ctttaattgc
     2341 ttcgaccaat aaagcacctg cacttttcca ttttaacttc gtaattaaaa ttgtaataat
     2401 tactgttaat aaaatcgctg tcccagttgc accaattaaa tcgagacgca acgcaattcc
     2461 tttaggcgat aaatcactca cagtatttgg aattggcaat tttattacta aactttcaag
     2521 tgcacctcca ggttggaata attttttgaa gaatggtgca ctccatacta atacaaaggc
     2581 agttaaaatt acgaacggac tccaagcaaa gacaatttct ttaggcgttc gtttttgaat
     2641 tttatgttca gacgcttcca atctgaaaat gtttttcggt ttaaatttac gacaaacaaa
     2701 tgctaacacc accattgttg ctagtgatgg aataatgtct gctagttctg gaccatggaa
     2761 tattgttaat aataattgta atccagtata tgtaccactc actgttaaaa tgacaggtaa
     2821 aatttcttta atacctttca taccatctac aatgaatact aaaacaaatg gaataataaa
     2881 gtttaaaatt ggaagtgtta atgctgagta tctcgcaaca tctaatgttg taacgcctcc
     2941 acttaagtta aacgtatcaa taatactaac tggtaaacca attgcaccaa aggcacccgc
     3001 cgcaccatta gcaattaaac ataacatcgc tgcttttaat ggttcaaatc caagttgaat
     3061 taataatact gcacaaatcg caattggcac accaaatcct gctgcacctt ctaaaaatgc
     3121 gttgaaacaa aatccaatta ataatagttg gattctttgg tccactgaaa tacttgcaat
     3181 actatcttga ataatagaaa attgtcctgt tttaatagaa actttatata accaaactgc
     3241 cattaaaacg atatatccta ttgggaaaat accggcaaca acgccttctg taatcgcacc
     3301 tgctgataca cgcgctggta attcaaatac aaataaagcc acaatcaatg taacaaccaa
     3361 agttgtcaat gctgcataaa tgcctttcat tttaaaaacg gttaagcata ataaaaataa
     3421 aataataggt actgctgcaa ctaaggctga taatccgaca ttatcgaatg gatttacagt
     3481 aagtagtgtc ataatgactc cctctcttta tataaaatat ttatcattct gattaatcta
     3541 caacctattt caacttatat tttgcgatga tcacatattt aaaatgtaac actcctatat
     3601 gtgacaggca atcgaatttt tacaaaaagt tcacaaaata tacacaatat ttaactataa
     3661 taaataatat atcatt
//
LOCUS       DAMQYT010000212         3639 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMQYT010000212 DAMQYT010000000
VERSION     DAMQYT010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.216"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(141..1202)
                     /locus_tag="PGA33_002405"
     CDS             complement(141..1202)
                     /locus_tag="PGA33_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDG6474196.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKFEVPKQ"
     gene            1695..2897
                     /locus_tag="PGA33_002406"
     CDS             1695..2897
                     /locus_tag="PGA33_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDG6474197.1"
                     /translation="MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPS
                     ACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIV
                     DESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLP
                     EHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVK
                     TVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPDYT
                     IFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRHSYELVQGLR
                     KDLRLCNWPKFINRLNSVSKKSVSKGVWKVVKYYRKHQRMLRNTIYYPAFNNGAIEGI
                     NNKIKLIK"
     gene            3033..3137
                     /locus_tag="PGA33_002407"
     CDS             3033..3137
                     /locus_tag="PGA33_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474198.1"
                     /translation="MYFKNSSLNYIPTTFGGEPKKKHFPKMESASSEP"
     gene            complement(3128..3200)
                     /locus_tag="PGA33_002408"
     tRNA            complement(3128..3200)
                     /locus_tag="PGA33_002408"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3165..3167),aa:Lys,seq:ttt)
     gene            complement(3204..3278)
                     /locus_tag="PGA33_002409"
     tRNA            complement(3204..3278)
                     /locus_tag="PGA33_002409"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3244..3246),aa:Gln,seq:ttg)
     gene            complement(3288..3371)
                     /locus_tag="PGA33_002410"
     tRNA            complement(3288..3371)
                     /locus_tag="PGA33_002410"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3335..3337),aa:Tyr,seq:gta)
     gene            complement(3401..3476)
                     /locus_tag="PGA33_002411"
     tRNA            complement(3401..3476)
                     /locus_tag="PGA33_002411"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3441..3443),aa:Val,seq:tac)
     gene            complement(3484..3555)
                     /locus_tag="PGA33_002412"
     tRNA            complement(3484..3555)
                     /locus_tag="PGA33_002412"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3520..3522),aa:Glu,seq:ttc)
     gene            complement(3557..3631)
                     /locus_tag="PGA33_002413"
     tRNA            complement(3557..3631)
                     /locus_tag="PGA33_002413"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3597..3599),aa:Asn,seq:gtt)
BASE COUNT         1356 a          635 c          563 g         1085 t
ORIGIN      
        1 tacataaaaa atccgctatt atacgtgaac aaatctcaca ttttaaaatc aatcaaaaaa
       61 gcgctactat caatatctta ttgacagtaa cgcctatatt tatcatatat taaaacttat
      121 tcttgtcatc aagctataaa ttattgtttt ggaacttcaa atttataaat atcattagaa
      181 tatataggaa tgaatttttt acacaattca tgaacaataa atgccgatac aaatggtatg
      241 acaaaataac caagtgctaa taaaataata ttcattgttg gatcacctga cattcggtta
      301 aatgcattta ttgggccaac taatcctgta tatccaaatc cagcagacaa tggtgttcct
      361 ttaacctgaa gaacgtaagc aatgattcca gtaattattc catttatagt caatggtatc
      421 gaaataatta aatttttcaa gtaaactggg atcatcattt ttgcagctcc tatgagtaaa
      481 actgcattta caccaataga attgacacgc aatgagccaa ataagaaagt aacacaggca
      541 gccactatac ctaggtttgc tgctccactt cctagtccgt ttaaactaat cgcagttgca
      601 atcgctacta acgatattgg tgttaccatt aataatgaaa atgccacact aataagtata
      661 gacattaaca acggatttaa gtctgtaaaa gaatgaatta catttccaat tgcttgagta
      721 atttttcgaa tgtaaggtaa tgtgattaga ccgatacccc cactaacgat aggtactaaa
      781 actggtaata taattaattc aaaagatcca agtttgtttt gtaataccat atatataaga
      841 catgcaataa taacaaccaa actcgtattt ataatgtcac ctataccttt taacataaaa
      901 ctattattgc tatatacaac agcacctgaa ccaatcatag ctgatgtacc tactatagca
      961 gcacctgcac cattaaattt aaattgatga gcagctaaaa ccccaataat aaatgccata
     1021 aatgattgaa ttagtatcac taactgatac gttaattcta aaatttcatt accactttta
     1081 aatattttta atacttcacc taataaagca ttcggaacaa gtgcaataac aacaccagca
     1141 ccaatagaat ttaaaatctt actgaaaaac tgtttattat cagcattatt tgcgttactc
     1201 ataaacgacc tccaatttga acattaaact catcatatta tagaaaatta actttaacaa
     1261 tgattagttg gctaatttaa tctaaatata acaatccaca aattgaataa agcaaattta
     1321 aacaatctac tcactaatca atgtcatgtt aattttaaaa tactactaat tacatcctgt
     1381 gactaataat gtaataaatt gtaaatatta aaatagccat attactatta agatagataa
     1441 attgaatcga cttggtataa agcacaataa aatagcactt ttacaaatat attattagaa
     1501 aagttgaaac aaatatataa ccccagataa tataatcatt atcattaatg taataccaaa
     1561 aaaagaagtt aaacaggttc ttcatctttt atggtgggaa ggtaaaactt cctgcttttt
     1621 ttaatacaca aaaagcgcaa ttgcctctat aatttaaagt gaccaaaccc aaactaaagg
     1681 agacaagtgc gcctatgtgt aatgatacct tagaattact aagaataaaa gatgaaaata
     1741 taaaatatat aaaccaagaa attgacgtca ttatcaaagg aaaaaaagca acagtggtta
     1801 atgctgtact aacgtataag ccttcggcct gttattgttg tggagttaaa aatgaaggac
     1861 aaattcataa acatggtaag cgtgtttctc gtattacttt acttaaaact caagggtata
     1921 acacatacct caacttagct aaacaacgtt ttaaatgctt agaatgcaat ggcactttta
     1981 ctgctaaaac gtcaattgtt gatgagtcgt gttttatctc aagatgtgtt actcaaaaag
     2041 ttatagaaga agctactaaa gttaaaacag agattgatac tgcagaagat aactgtatct
     2101 ctccatctac tgtaagtcgt attagaacta aagcggctaa ttcattacga attaaaccct
     2161 ttaattgttt gccagaacac atcgctatgg atgaatttaa aagcgttaaa aatgtaactg
     2221 gatcaatgag tttcattttt atagataatg atactcatga tgttatagat attttagaaa
     2281 atagaactac aagattcttg cgtgcctatt tcgagcgatt cgatttaaaa aatcgacaac
     2341 aagttaagac ggttactatt gacatgtatg aaccctatgt ccgattattt cgcgacctat
     2401 ttcctaatgc agctattatt tttgacagat tccatatcgt tcaacattta aatagagaac
     2461 ttaataagta tcgtgtacaa gttatgaatg aataccgtaa taaaaaagga cctgattata
     2521 caatttttaa gaataactgg aaagtcctat tgatggatac tagtaaaacc atatttagta
     2581 aatacagatg gaataaatct tttaaggctt ataaacgctc atctgacatt gtagaattca
     2641 tgctttcaaa agacgatata ctacgacact cctacgaact tgtccaagga ttacgaaaag
     2701 acctaaggtt atgtaattgg cctaaattta ttaatcgttt aaattcagtt agtaaaaagt
     2761 ctgtgagtaa gggtgtatgg aaagtggtta aatattatag aaaacatcaa aggatgttaa
     2821 gaaatacaat ttattaccca gcatttaata atggtgctat agaaggaatt aataataaga
     2881 taaaattaat caagtgaatt tcttttggtt acagaaattt caacaacttt aaagcacgta
     2941 taatgatgat tttcagcttg tacaaaggag aaaaaaagaa gacaaccaag cccaataatg
     3001 gactggccgc ctaataataa aagctctaaa agttgtattt taaaaatagt tctttaaatt
     3061 atatacccac cacatttggt ggagaaccta aaaaaaagca cttcccaaaa atggaaagtg
     3121 caagtagtga gccatagagg attcgaacct ctgaccctct gattaaaagt cagatgctct
     3181 accaactgag ctaatggctc taatggctgg gctagctgga ttcgaaccaa cgagtgacgg
     3241 agtcaaagtc cgttgcctta ccgcttggct atagcccaat atatagatgg tggagggggg
     3301 cagattcgaa ctgccgaacc cgaaggagcg gatttacagt ccgccgcgtt tagccacttc
     3361 gctacccctc cagcttattc atataattta ataatcaaaa tggtggagaa tgacgggttc
     3421 gaaccgccga ccctctgctt gtaaggcaga tgctctccca gctgagctaa ttctccaaaa
     3481 taatgactcc tacgggactc gaacccgtgt taccgccgtg aaagggcggt gtcttaaccg
     3541 cttgaccaag gagccatggc tccacaggta ggactcgaac ctacgaccga tcggttaaca
     3601 gccgatagct ctaccactga gctactgtgg attaatatt
//
LOCUS       DAMQYT010000213         3614 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMQYT010000213 DAMQYT010000000
VERSION     DAMQYT010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.217"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(118..267)
                     /gene="rpmG"
                     /locus_tag="PGA33_002414"
     CDS             complement(118..267)
                     /gene="rpmG"
                     /locus_tag="PGA33_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDG6474199.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            complement(358..1875)
                     /locus_tag="PGA33_002415"
     CDS             complement(358..1875)
                     /locus_tag="PGA33_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDG6474200.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKSHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            2079..3533
                     /locus_tag="PGA33_002416"
     CDS             2079..3533
                     /locus_tag="PGA33_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDG6474201.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
BASE COUNT         1103 a          656 c          564 g         1291 t
ORIGIN      
        1 accgatgaaa ctcaatccat taatcactct tcacaaaaat ttcataaaaa agcgagctcc
       61 ccaatataat aggtaagccc gctttatttt caaatcgtcg tatttgaatg ataagaatta
      121 cttagtttca cgatgtaacg tatatttgtt taatcttggg caatattttt tcatttcaat
      181 acgctcagga ttattacgtt tatttttagt agtgatatag ttacgatcgc cacattctgt
      241 gcatgctaat gttacgttta cgcgcacgtt aaacaccctc tcttgttagg aatctttacg
      301 atttattatg atatcataaa ctgctcaact acgcaatata gaaactacat atcaaattta
      361 tttttcaaag ttttcgtatg tttcatcatt ttcagtttca agatcaatag aatttatatc
      421 aatacctaat gcttttgcaa cacctttacc atattctggg tcagctttgt aacaatgacg
      481 aatatgacgt cgtttaacat catccgttac gccttccatt gcatttgctg tatttgtaaa
      541 aattctttct ttagcgtcct ctgattgtaa tctaaacaat ttacctggtt gttcaaaata
      601 attatcatca tcttgacgtt gattatattc atagccgtat ccatcagttg ggaatggtgg
      661 ctttttatat tcaggttgag aatcaaattt accatggtta tttggataat aatgtgttcc
      721 tccaccttgg ttattgtcaa ctacgcgcat ttgaccatct ctactaaaag gacaaatatt
      781 ttcaatacca acacctttag gttggtttac aggaatctgc caatgattaa ctcctaatcg
      841 atatctttgc gcatcgccat atgagaataa acgcccttgc agcattttgt ctggagaaaa
      901 atctaatcct ggaataatat tagttggtgc aaacgcagct tgttcaacat ccataaagta
      961 attatctgga tttctattta attcaaactc tccaacttca attagaggat actcatcgtg
     1021 ataccatact tttgttaaat caaatggatt atctttatgg tttttagctt gttcctcagt
     1081 cattacttga atatacattg tccattttgg ataatcacct ttttcaatgg cttcgaataa
     1141 atcgcgttga gatgaatcac gatctgtagc tataatttca gcagcttctt catcagttaa
     1201 gttttcaata ccttgttgcg ttctaaaatg ggatttaacc caaacacgtt caccagaatc
     1261 attatacata gagtatgtgt gagaaccgaa cccatgcata tgacgtaaat ctttaggaat
     1321 ccctctatct gacattaaga tcgttacttg gtgcaatgct tctggaagac ccgtccagaa
     1381 atcccagtta ttttgtgcat ctctcatatt tgttctagga tctcgtttca ccgcacgatt
     1441 taaactaaca aataactttg gatctctaaa gaagaatact ggtgtgttat tccctactaa
     1501 atcccagttc ccttcttcag tgtagaactt taacgcaaat cctcgaatgt cacgctccgc
     1561 atcagcagca ccacgttctc ctgctacagt agagaaacgg gcaaacattt cggtttgctt
     1621 acctatttca gagaatattt tagcattcgt atattttgtt atatctttag ttacagtaaa
     1681 tgtcccaaat gcaccagaac ctttggcatg cattcgacgt tctggtatta cttctctatc
     1741 aaattgagac atttgctcta aaaagtaaat atcttgcatt aaaagaggtc ccctaggccc
     1801 tgctgtcata ctattttctc ggtctgatac tggatgccca aaaacaccag ttaacttttt
     1861 gtcttgttgt gacatagtca tccctccaca atttataata attataattt aatacatatt
     1921 ataatttaac cccatcaaaa ttacaattaa acatgctcaa gatgattaaa aaatcagaaa
     1981 attttaattt tttcgcttta cagtactaaa tagttattga atagtacact taaaaagctt
     2041 acaataatgg gtatatttaa atagatggga gactaaatat ggaagataat aaaatgaacc
     2101 gtagtcttaa ctcaagacac atttccatga ttgctatagg tggtgcaatt gggactggtc
     2161 tatttgtagc tactggtaat atcatttctc aagctggtcc tggaggcgct atactcgctt
     2221 atcttgttat tggtgtcatg ctatatttct taatgtcatc aattggagag ttggcaacat
     2281 tttatccagt atcaggttca ttcagctctt attcaacacg ctttattgac tcatctcttg
     2341 gctttaccat gggatggttg tattgggcat tgtggtcatt agttacaagt gttgatgtca
     2401 tagtagcgtc aaatgtgctt tatttctggg acacatttaa atttttccac cccattactt
     2461 ggagcttaat ctttattaca attttactat tattaaacat tttttctgta aaatcatttg
     2521 gagaaactga gttttggtta tcattgatta aagtgttaac aattatcgta ttcgttattt
     2581 ttggcttttt aatgattttc ggtatcttag gtggtcatac atatggattt gaaaactata
     2641 caaaaggcca agcaccgttt gttggtggta tctctggttt cttaggcgta ttattagtcg
     2701 ccggattttc ggttggtggt acagaagtag tagcagtaac tgctggtgaa tcagatgacc
     2761 ctaaaaagtc tatgcctaag gcaattaaac aagtattttg gcgtattctt ttattctatg
     2821 tcttatcaat tgcagtaatt ggtgcaatta ttccgtacac agatccatca ttattaagag
     2881 caagtagttc aataagtcaa agcccattta caattgtatt cgatagagta ggcatagcct
     2941 ttgcagcatc agtaatcaac gcggttattt taacttcatt attatccgct gcaaattcag
     3001 gtgtttatac aacaggcaga atgttgtatt ccttaagttc agacaaaaaa gcaccccaat
     3061 ttttaagtaa attaaacaag acaactaagt tacctttaag agcattatta actacttatg
     3121 cagtcgttgt tattgttatt atttatgcaa actttaattc aaatgccgtt tttaatttac
     3181 ttgaaattat tggttcaatg attatagttg tttggggatc aagcatttgg tcacaaatac
     3241 gattgcgaca agctattaaa aaacaaggtc aagaccctaa taaggtccta ccatataaag
     3301 caccttttta tccattagga ccaatcattg tcatcactac actattattc ttgctatttg
     3361 gtggctcagt tgaatatatt ttaaaagatc aatggttaaa tgcttttaaa aactttttac
     3421 ctttaatcat tctagcgttg atttacttta ttcataaaat cattcacaaa acaaaatttg
     3481 taaagctaga aacaattaat ttaaaaccac acgattatga caatcaaaaa taaataaatt
     3541 ctcatacggc agatgcttta gattatctat agcatttgcc gctttttaat ggtgtaaagc
     3601 agatatactt taat
//
LOCUS       DAMQYT010000214         3595 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMQYT010000214 DAMQYT010000000
VERSION     DAMQYT010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.218"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..411
                     /locus_tag="PGA33_002417"
     CDS             <1..411
                     /locus_tag="PGA33_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474202.1"
                     /translation="QYRHTKTVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYN
                     SKAHPDLTVKVREKDNIVKGIILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVG
                     NDFSSVKYIDRDNHLKLNLLYQDLEIKRIEFFSK"
     gene            471..1028
                     /locus_tag="PGA33_002418"
     CDS             471..1028
                     /locus_tag="PGA33_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDG6474203.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            1025..1867
                     /locus_tag="PGA33_002419"
     CDS             1025..1867
                     /locus_tag="PGA33_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDG6474204.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
     gene            complement(1924..2964)
                     /locus_tag="PGA33_002420"
     CDS             complement(1924..2964)
                     /locus_tag="PGA33_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDG6474205.1"
                     /translation="MQQVTSDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYQPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            3415..3588
                     /locus_tag="PGA33_002421"
     CDS             3415..3588
                     /locus_tag="PGA33_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474206.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
BASE COUNT         1263 a          451 c          620 g         1261 t
ORIGIN      
        1 caatatcgtc atacgaaaac tgtggatttg tctaatcttg aaataaacga tattaaatta
       61 aatgagaagt ttgataaaaa aggctatgaa gtaaataaaa aaattaagtt tgatcgtttt
      121 aaattttata acagtaaagc tcaccctgat cttaccgtta aagtgagaga aaaggataac
      181 atcgttaagg ggataatatt agtaagagat gaaaagatac atactaattt tgatggggga
      241 attggttcgc cgataaataa cgcgattgaa aatcttggat tcggatataa aagaacaaaa
      301 gttggcaatg atttctcatc ggtaaagtat attgatagag ataaccattt aaaattaaac
      361 ttactttatc aagatttaga aattaaacgt attgaatttt ttagtaaata gctttaggtc
      421 ttaaagttat aaaaaacgaa tgaataattt tattgggatg agtgacctat atgaacaaga
      481 ttacttattt aaatgaatta gaaacagcac ttgatgcgct acctaggcat ttaagagatc
      541 aaaaaatgta tgaatatgaa cgttattttt atgagcaaga acttaatggc gttgatgaag
      601 gggaaatttt aaagaagtta aaagacccac aagatgttgc agctgaaaca aaagctagaa
      661 gtgtcattga ttatgctgaa tctaaaccaa catttgaaaa tatttcaaga gctgttgctg
      721 cttcattaag tttaggcatt ctatctattt ttgtcatcct tataccagta tctatagttg
      781 gattatttgt attagcatta tttttaatat cacttttgct gctgttttgt ccaattattt
      841 tattagcatc agcaatatcc agaggaattg tggactcaat tagtaatgta ttttttgcca
      901 tatcatattc aggattagga ttagtattta tcattgtcat atttaagatt ttagaataca
      961 tttatcgttt aatcttaaaa tatttacttt ggtatattaa aactgtcaaa ggaagcgtta
     1021 gaaaatgaag aaattctttt ttattgggct tttagtgttt gttgtctttt ttacagcagc
     1081 aaccattatt tggttcagct atgataaaaa caaatatggt actaaacaat atgataaaac
     1141 attcaaagac gatgcttttg acaatgtatc tataaatttg gatagtacag aacttcgtat
     1201 aaaacggggg aatcaattta gagttaaata tgatggtgac aatgatatat taattaatat
     1261 agtagataag acgttgaaga ttagtgataa aaggtctaag acaagaggat atgcaattga
     1321 tatgaatcct tttcatgaga ataagaaaac gttaacgatt gaaatgcctg ataaaatgat
     1381 taaacgttta aatctatcat ctggagcagg aagtgttaga atcagtgatg ttgatttaga
     1441 gaacacaagt attcaaagca ttaacggtga agtagttatt aaaaactcaa atcttgacgc
     1501 actagattca aagacaaata atagttctac ttatattagt aaaagtaaca ttaaaaatag
     1561 caatattaaa gttgttattg gtacgctaca aatcgacaag agtcaaatta aacaatccat
     1621 atttttaaac gatcatggtg acattgaatt taaaaacatg ccatcaaaag tagatgcaaa
     1681 agcttctact aaacaaggag atattcgttt taagtatgat agtaaacctg aagacactat
     1741 actaaagcta aatccgggaa cgggtgatag cgtagttaaa aataaaacat ttactaatgg
     1801 taaagttggg aaaagcgaca atgttttaga attttatacg attgatggta atatcaaagt
     1861 tgaataaata aaggatgtaa gcaccgatat taggaagcat aatttctcta atatcggtgt
     1921 tatttatttg ttggcaaaag ttaagtcggt atctatattg ccagtaaagt gagtgatatt
     1981 aaggtcttga ccatctaacc atgatttgaa atctattatt tctggtggcg cattttctcc
     2041 caatgtaaaa tatgcagtta atgtttcagg ttgatacatt gatgtatgga tggtgccaga
     2101 ccagcttttg aatagtttac tgtaaatttc atactgagga ttattgaata acttaaatgc
     2161 tgtagtcata tctaaattat cattagtttg tgaaatggta cgcgccagtc tttctttaga
     2221 ttcttttgta taattacgat tttcatgtgt taatatttca aaatgatttg tacatatatt
     2281 atcataacga acatctattg atctcggtgt cacttcaaca attgcatggt tcaatgattt
     2341 gtccatcagt atgtagctaa atgagcttct gtgtggtatt tctttcaata attggattgc
     2401 ttctgttaca tttcggcaat tttcaagaat tagacgacca atcatataac atacaaaacc
     2461 atttgctggt ttcttccggt gcataaagtt atagcccata gttaatcctg actcattcat
     2521 accatccatt cttccagtta cccttgatac aggaccaatt tgagctaaac cgctatctgt
     2581 aggttgataa agtaagtagc gaccatcata agttgcaggg tggtaatcat aatttctaac
     2641 catgaagtct ttgccttgaa agaccgtgca accactttct tttaaatcgg taaaacgata
     2701 atgtccaaag tttaaaataa tttggcgtgt tggcattttg agtatacttt gtagtcccat
     2761 taattcttcc catatttgag gtgcgtatgt ttggaatatt tgataagttt catttacatc
     2821 tatatcgaaa cgtgggacac gttttttcca ttctttttct cgatttttta gaagaggtgt
     2881 ttgttgaagc catttaccag ttttaacacc taactcgaaa tgtgaacctc taaaagtcat
     2941 gatatctgat gtcacttgtt gcatatcatc ggcccctttc tttttagttg taatatattg
     3001 taaataaata gtaatcgtat gtatattgaa tgtcatgtta aataaagtta tattttacta
     3061 aatgaaatat aaaattgttt gaggtgattt ctcggtgtat aagacttatc aatcagttaa
     3121 aacatatttt tatagatggt ggggatattg agttaaaaac ttaaaatcat cttatcataa
     3181 atatcaatct taagttagca ttcacgataa tagtcattgt taacattagc atataaggtc
     3241 atgtcacgtt gaaacagagg ttcctcggca tttttgaaaa taatcattag cttgctcact
     3301 atataatttg atgaatatat ttcgtgaaag tgggtattta tttaatgatt attctatata
     3361 tgatagtata atgaaatgta gataggtatt taatttaaca gaggtgaaat tgagatgtgg
     3421 aattttatta aatgtgtgtt taaattcgta tttagcttag ttgctattac aacattagtt
     3481 gctggtgttg gtgtagtagc atttgcttat atctttaaaa aagattttga agatattgaa
     3541 agaaaaacta aagaaattat ttctgatatt gaaagtaaaa ataactaata acatt
//
LOCUS       DAMQYT010000215         3592 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMQYT010000215 DAMQYT010000000
VERSION     DAMQYT010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.219"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            228..530
                     /gene="gatC"
                     /locus_tag="PGA33_002422"
     CDS             228..530
                     /gene="gatC"
                     /locus_tag="PGA33_002422"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDG6474207.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            532..1989
                     /gene="gatA"
                     /locus_tag="PGA33_002423"
     CDS             532..1989
                     /gene="gatA"
                     /locus_tag="PGA33_002423"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDG6474208.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            2002..3429
                     /gene="gatB"
                     /locus_tag="PGA33_002424"
     CDS             2002..3429
                     /gene="gatB"
                     /locus_tag="PGA33_002424"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDG6474209.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            3469..>3592
                     /locus_tag="PGA33_002425"
     CDS             3469..>3592
                     /locus_tag="PGA33_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474210.1"
                     /translation="MIADLDAIEGLFISIEVKLLLTLFIVHVNGKNINFINALAL"
BASE COUNT         1326 a          522 c          674 g         1070 t
ORIGIN      
        1 tttttggata tgagcaaatt tttataatta aaattgcata atcataatat tgaattgggt
       61 tgtctaatat taaaagagtg ttgttttcat aaaaaatgat ttgataaacg aatgataatg
      121 tttatacagt ttatatgaaa ttaaagtagc acctcataaa tacttagatt tttaattgga
      181 aatttgatac aatttagtga tgaatgactt aaaggaggct tttattaatg acaaaagtaa
      241 cacgtgaaga agttgagcat atcgcgaatc ttgcaagact tcaaatttct cctgaagaaa
      301 cggaagaaat ggccaacaca ttagaaagca ttttagattt tgcaaaacaa aatgatagcg
      361 ctgatacaga aggcgttgaa cctacatatc acgttttaga tttacaaaac gttttacgtg
      421 aagataaagc aattaaaggt attccacaag aattagcttt gaaaaatgcc aaagaaacag
      481 aagatggaca atttaaagtg cctacaatca tgaatgagga ggacgcgtaa gatgagcatt
      541 cgctacgaat cggttgagaa tttattaact ttaataaaag acaaaaaaat caaaccatct
      601 gatgttgtta aagatatata tgatgcaatt gaagagactg atccaacaat taagtctttt
      661 ctagcgctgg ataaagaaaa tgcaatcaaa aaagcgcaag aattggatga attacaagca
      721 aaagatcaaa tggatggcaa attatttggt attccaatgg gtataaaaga taacattatt
      781 acaaacggat tagaaacaac atgtgcaagt aaaatgttag aaggttttgt gccaatttac
      841 gaatctactg taatggaaaa actacataat gaaaatgccg ttttaatcgg taaattaaat
      901 atggatgagt ttgcaatggg tggttcaaca gaaacatctt atttcaaaaa aacagttaac
      961 ccatttgacc ataaagcagt gccaggtggt tcatcaggtg gatctgcagc agcagttgca
     1021 gctggcttag taccatttag cttaggttca gacacaggtg gttcaattag acaaccggct
     1081 gcatattgtg gcgttgtcgg tatgaaacca acatacggtc gtgtatctcg atttggatta
     1141 gttgcttttg catcttcatt agaccaaatt ggtccattga ctcgaaatgt aaaagataat
     1201 gcaatcgtat tagaagctat ttctggtgca gatgttaatg actctacaag tgcaccagtt
     1261 gatgatgtag actttacatc tgaaattggt aaagatatta aaggattaaa agttgcatta
     1321 cctaaagaat acttaggtga aggtgtagct gatgacgtaa aagaagcagt tcaaaacgct
     1381 gtagaaactt taaaatcttt aggtgctgtc gttgaggaag tatcattgcc aaatactaaa
     1441 tttggtattc catcatatta cgtgattgca tcatcagaag cttcgtcaaa cctttctcgt
     1501 tttgacggaa ttcgttatgg ttatcattct aaagaagctc attcattaga agaattatat
     1561 aaaatgtcaa gatctgaagg tttcggtaaa gaagtaaaac gtcgtatttt cttaggtaca
     1621 tttgcattaa gttcaggtta ctatgatgct tactataaaa aatctcaaaa agttagaaca
     1681 ttgattaaaa atgactttga taaagtattc gaaaattatg atgtagtagt tggtccaaca
     1741 gcgcctacaa ctgcgtttaa tttaggtgaa gaaattgatg atccattaac aatgtatgcc
     1801 aatgatttat taacaacacc agtaaactta gctggattac ctggtatttc tgttccttgt
     1861 ggacaatcaa atggccgacc aatcggttta cagttcattg gtaaaccatt cgatgaaaaa
     1921 acgttatatc gtgtcgctta tcaatatgaa acacaataca atttacatga cgtttatgaa
     1981 aaattataag gagtggaaat catgcatttt gaaacagtta taggacttga agttcacgta
     2041 gagttaaaaa cggactcaaa aatgttttct ccatcaccag cgcattttgg agcagaacct
     2101 aactcaaata caaatgttat cgacttagca tatccaggtg tcttaccagt tgttaataag
     2161 cgtgcagtag actgggcaat gcgtgctgca atggcactaa atatggaaat cgcaacagaa
     2221 tctaagtttg accgtaagaa ctatttctat ccagataatc caaaagcata tcaaatttct
     2281 caatttgatc aaccaattgg tgaaaatgga tatatcgata tcgaagtcga cggtgaaaca
     2341 aaacgaatcg gtattactcg tcttcacatg gaagaagatg ctggtaagtc aacacataaa
     2401 ggtgagtatt cattagttga cttgaaccgt caaggtacac cgctaattga aatcgtatct
     2461 gaaccagata ttcgttcacc taaagaagca tatgcatatt tagaaaaatt gcgttcaatt
     2521 attcaataca ctggtgtatc agacgttaag atggaagagg gatctttacg ttgtgatgct
     2581 aacatctctt tacgtccata tggtcaagaa aaatttggta ctaaagccga attgaaaaac
     2641 ttaaactcat ttaactatgt acgtaaaggt ttagaatatg aagaaaaacg ccaagaagaa
     2701 gaattgttaa atggtggaga aatcggacaa gaaacacgtc gatttgatga atctacaggt
     2761 aaaacaattt taatgcgtgt taaagaaggt tctgatgatt accgttactt cccagagcct
     2821 gacattgtac ctttatatat tgatgatgct tggaaagagc gtgttcgtca gacaattcct
     2881 gaattaccag atgaacgtaa agctaagtat gtaaatgaat taggtttacc tgcatacgat
     2941 gcacacgtat taacattgac taaagaaatg tcagatttct ttgaatcaac aattgaacac
     3001 ggtgcagatg ttaaattaac atctaactgg ttaatgggtg gcgtaaacga atatttaaat
     3061 aaaaatcaag tagaattatt agatactaaa ttaacaccag aaaatttagc aggtatgatt
     3121 aaacttatcg aagacggaac aatgagcagt aaaattgcga agaaagtctt cccagagtta
     3181 gcagctaaag gtggtaatgc taaacagatt atggaagata atggcttagt tcaaatttct
     3241 gatgaagcaa cacttctaaa atttgtaaat gaagcattag acaataacga acaatcagtt
     3301 gaagattaca aaaatggtaa aggcaaagct atgggcttct tagttggtca aattatgaaa
     3361 gcgtctaaag gtcaagctaa tccacaatta gtaaatcaac tattaaaaca agaattagat
     3421 aaaagataat ttaaatcatc aaactatgaa gatttaaaaa ataaaccctt gattgctgac
     3481 ttagatgcaa tcgagggttt atttatatct atagaagtca aattactttt aactttattc
     3541 attgtacatg ttaatggtaa aaatattaat tttattaatg cgttagcttt aa
//
LOCUS       DAMQYT010000216         3574 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMQYT010000216 DAMQYT010000000
VERSION     DAMQYT010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.220"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..297
                     /locus_tag="PGA33_002426"
     CDS             <1..297
                     /locus_tag="PGA33_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDG6474211.1"
                     /translation="PQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLNNLIEQDH
                     RHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIMLAS"
     gene            355..732
                     /locus_tag="PGA33_002427"
                     /pseudo
     CDS             355..732
                     /locus_tag="PGA33_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            729..1610
                     /locus_tag="PGA33_002428"
                     /pseudo
     CDS             729..1610
                     /locus_tag="PGA33_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1726..2655
                     /locus_tag="PGA33_002429"
     CDS             1726..2655
                     /locus_tag="PGA33_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6474212.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFEDLVGGGTIG"
     gene            2648..3385
                     /locus_tag="PGA33_002430"
     CDS             2648..3385
                     /locus_tag="PGA33_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6474213.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKV
                     PTPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLKNLLIIPISRESFL
                     VSKIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSP
                     PIIILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFL
                     LSLIIIIVTSIFSVIILFSYFKKTDIS"
     gene            3399..>3574
                     /locus_tag="PGA33_002431"
     CDS             3399..>3574
                     /locus_tag="PGA33_002431"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="BacA; phosphatase activity in Escherichia coli not
                     kinase; involved in bacitracin resistance as bacitracin
                     supposedly sequesters undecaprenyl disphosphate which
                     reduces the pool of lipid carrier available to the cell;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase"
                     /protein_id="HDG6474214.1"
                     /translation="MGFIEQIKAFILGVVEGLTEFAPVSSTGHMIIVDDMWLNTADDL
                     GSASATTFKVVIQLG"
BASE COUNT         1308 a          491 c          632 g         1143 t
ORIGIN      
        1 cctcaaaagg tagttacaga tcaggcacct tcaacgaagg cagcaatggc taaaataatt
       61 aaagctttta aacttaaacc tgactgtcat tgtacatcga aatatctgaa taacctcatt
      121 gagcaagatc accgtcatat taaagtaaga aagacaagat atcaaagtat caatacggca
      181 aagaatactt taaaaggtat tgaatgtatt tacgctctat ataaaaagaa ccgtaggtct
      241 cttcagatct acggattttc cccatgccac gaaattagca tcatgctagc aagttaaacg
      301 aacactgaca tgatatatta gtgattagct atatttttta ctttgcaaca gaaccagcca
      361 tttaatatga ctgttacaat tactaagatt caagcactat tacgccgcac gtatgatttc
      421 accactcaag ttaataattt aacagtagat gattgcgagt tactacttga tgcagcaaag
      481 cttaaattta atgaagaaac catagacctg acacacactg aattgcaaat catgcatagc
      541 ttattccaga acaagggcaa attcgtcagt cgtaatgcgt taatcgaaaa atgctgggaa
      601 tcagaacact tcattgatga caatacgctt gctgtcaaca ttacacgttt acgcaaaaag
      661 ctacaaaaac taggacttac agattttatc gaaacgaaga aaaatgtcgg atatcgtgta
      721 ggtgacgtat gaatttcttg aaatcaatcc aacgtgaaat ttatattgtc atccttactt
      781 gtgctttatt ctactttata ttattcatgt ataatgtgcc gatgcaagca tatatgattg
      841 gtctaggtat tgtattgttt gtttcaatga tttactgggg tatccattac atgaacttta
      901 aaaaagaata aacactacag gaacagaacg aagatttaag aatcgaaaac cataaacttc
      961 gtaatgactt tattaactac caaagtgagt tagagtcata ttttttaatg tgggtgcatc
     1021 aaatcaagac accgattaca gcatcaaagt tactgcttga agatcctgat gaacacgtga
     1081 tttctaacgt gcgtaatgaa gtcttacaaa ttgataatta cacgaatctt gcactgaact
     1141 atttgaaact catgaaccaa aagacataca tggtctttat ggaagtgagt gtagatgaat
     1201 taattcgacc actgattaaa cgttatgcga tacagtttat tcatcatcaa acaaagttgc
     1261 attatgagaa aagtgaagcg gttatattaa cagatgccaa atgggcatca attatgattg
     1321 agcaaatatt aaacaatgca ttaaaatatg cacgtggcaa agatgtgtgg attacgtttg
     1381 acgaaaatac aaagaaatta tcaatccgag ataacggtat cggcattaat gcgagtgatt
     1441 taccgaaaat ctttgataaa ggtttctcgg gctataacgg tagaataagt gagaaatcaa
     1501 gcggtattgg tttatatatt gtcagcacaa tctcaaagcg cttaggccat aaagtaagtg
     1561 ctacatctac attaaacgat ggttcaatat tcaccattca atttaaataa ctccaatctt
     1621 tcatttttgt aagattagcg gctgtttttg taagaataga taaaggcagc cttttctaat
     1681 cgcgcgtaag ataaaggtaa caacaaacta agaaaggtgc cagaggtgaa aaatttgaat
     1741 aatataattg aaataaataa cttaacaaag caatatggaa gtcagatagc agttaataat
     1801 ttgagtataa ctgttgaaag tggacaaatt tatggttttt taggaagaaa tggtgcaggc
     1861 aaaacaacta ctataagaat gcttttaggc ttaattaaac ctacgaaagg taatattaaa
     1921 atatttgaaa aagagttcga aagtaataaa actgaaattt taaaaaatat tggtgctatg
     1981 gttgaaacgc caggttttta tagtaatttg acagctaaag agaacttaac tataaatgct
     2041 aaaatattag gtatacaaaa agaaaatgca attgaagaag tgttagaaat agtaggacta
     2101 gcttctgaaa aagtaaaatt gtttaaaaat tattctttag gtatgaagca acgtttaggg
     2161 attgcgagag ctttattgca caatcccgaa ttattgatac ttgatgagcc gacgaatgga
     2221 ttagatccta ttggcataaa agaaattaga aagttgatta aggatctggc tcaatctaga
     2281 aatataacca ttttaatttc aagtcatata ttgtcagaaa tagagcagtt agtagacaag
     2341 gtgggtatta ttcataacgg agttttgtta gaagaaatta tgtatgaaga tttaaaaaaa
     2401 aagaacagac aatttataag tatatcagta tcagatgaag aaaaagttgc atgtttgttg
     2461 gaaactaact tcggaattta tgactataaa attaaagaaa atggcgaatt taaaatttat
     2521 tcacatattg atagacaaca agaaattaat agattattag tattaaacaa tattgatgtt
     2581 ttccagatga gagtgataga agataatcta gaaacttatt tcgaagactt agtaggaggt
     2641 ggcacaattg gataatctac tccgtacaga atttattaag ttaaaaagat catatatgtt
     2701 tctaataagt attttaggta catgcgtagc accttttatg gtggttgtag cttcttttat
     2761 tagtatgcgg acgaaagtac cgactcctaa tattgtattt aaacagttat ttgaagaagt
     2821 cagtttatat catatgctaa ttattggacc tcctctatat ggggttataa tagcctattt
     2881 gtttggaaga gaatatatag aagatacttt aaaaaactta ttaattatac ctatatctag
     2941 agaaagtttt ttagtaagta aaataatatt attattcttg tggattcaaa cactatctgt
     3001 tattgcatgg ttgctaactt taatgctagg tatgctattg cattttcaag ggttaagcca
     3061 gagtttaata attgattcat tctttacatt tgttattggc ggagtgttat tatggatatt
     3121 atctccacca ataattattt tgtcgatata ttttaaaaat tatgttccca caattgtttt
     3181 aaccataata attactttga taaatttaat gactgctaat tctgaacata gaggattatt
     3241 tccttgggct gcagttggag acatatctag aggaacatta cctaatactt atccatttct
     3301 attatcttta attatcataa ttgttacatc tatattcagt gtaattatat tattctcata
     3361 ctttaaaaaa actgatataa gttaaaagga ggagaaaaat gggttttata gagcagatta
     3421 aagcatttat tttaggtgta gttgaagggt tgacagaatt tgctccagta tcatcaacag
     3481 gtcatatgat tattgttgat gatatgtggt taaacactgc tgacgattta ggaagtgcgt
     3541 ctgcaactac ttttaaggtg gtaatacagt tagg
//
LOCUS       DAMQYT010000217         3525 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMQYT010000217 DAMQYT010000000
VERSION     DAMQYT010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.221"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            49..462
                     /locus_tag="PGA33_002432"
     CDS             49..462
                     /locus_tag="PGA33_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474215.1"
                     /translation="MKPTKVILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDCDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINVAKSDFTKFINELASLH"
     gene            complement(952..1938)
                     /locus_tag="PGA33_002433"
     CDS             complement(952..1938)
                     /locus_tag="PGA33_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDG6474216.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            2466..3224
                     /gene="map"
                     /locus_tag="PGA33_002434"
     CDS             2466..3224
                     /gene="map"
                     /locus_tag="PGA33_002434"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDG6474217.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
BASE COUNT         1244 a          534 c          635 g         1112 t
ORIGIN      
        1 atggtgatta tgcaacatga aaaaatgaat gatgaaagga atttgacgat gaagcctact
       61 aaagtgatat taaaagatgc atcttattta catagcaaaa catcgataac atttatttta
      121 aaagatgtag ttatcgaaga agataataaa atttattatt tcgacactag tgcaactttc
      181 gaagatcaag aagttaaatt tgaatttgca ctctttgata gtgacatgga caatttgaaa
      241 catatggatt gcgacaatcc tgttactgaa atatatttta tagaacctga tttacatttt
      301 acaattattg attttaatca agaactgctt tgtatttata ttgattttga ttctggttta
      361 aggcattcaa acatggcaac agaatctggt atttcattaa ggataaatgt tgctaaatca
      421 gattttacta aatttattaa tgaattagcc tctttacatt aatgatttaa atctgatatg
      481 taattacaat caaaaaagac agccacatcc ctccgtagtt taggcgtgtg gctatatttg
      541 agtctgaata tttatgcttg taattttaaa aagggacatg ctatatacga taaaaagagg
      601 cggggacata aatcaatgtt ctatgctcta cgaagttata ttggcagtag ttgactgaac
      661 gaaaatgcgc ttgtaacaag cttttttcaa ttctagtcag gggccccaac aaagagaaat
      721 tggattccca atttctacag acaatgcaag ttggggtgtg ggccccaaca aagagaaatt
      781 ggattcccaa tttctacaga caatgcaagt tggggtgtgg gccccaacac agagaatttc
      841 gaaaagaaat tctacaggca aagcgagttg gggtgggacg acgaaataaa ttttatgaaa
      901 atatcatttc tgtcccactc ccatggtgcc aattagcata aggtacttaa attaagcata
      961 tctgctgtct agcagtcgat aaatcattag aacttcgtat agtatatgac ttttaatttg
     1021 attttcacca ctaatttcaa gtgcttttat agtcgaacgt aaagtttcta cagaatcatc
     1081 ttctctctta aaagaaccat cataaaatat atctttgatg ctactactaa tttttagcaa
     1141 tgccattttt tcgtcacctg aaaagttaac acgagtattt ttaggcaagt aaatgatatt
     1201 tgataaatga gtgataaaca aacgattcgt atatgcacgt ttagttaatt gattgagtaa
     1261 tttccaatca cattcttttt tcttatgata gcttaattca tcacgttgat aacttattaa
     1321 cgtttcaact tgattattta aattgaaaat atttttatat gctttttcgc ttttatcaga
     1381 ttgcagtctt gataagataa gttcttggca gcgattgtaa aataatttat acatcaaggc
     1441 atctgtctta cttaattttt cttcgacctg accataatac ttaggtggaa acaccatgaa
     1501 gttaattaaa cctgatgtca cgagtccaat aattgctgtc aatgttcgag acaaaaagtt
     1561 gaatatgtag gcatcatgaa tacctggaat catagctaat gatgttagta cagcgacatt
     1621 cgtaccaact tgcaatttga gttttgtaca gaataaaatc gtgaacgttg cactcaatgc
     1681 atatgtaaaa ggtgattgat cgccgaataa atatgtaaat aatactgcaa agcctgcacc
     1741 aattaccgta gcaggtaatc tacgataacc tttaataagt gatgccttgg cagttggttc
     1801 aattgtgact acagctgtta aaatggcata gatgggtgtt aaatctagtg ccatacaaaa
     1861 gacagctgtt aaaaaaatgg caataccagt tttaattgtt ctggcaccaa ttaaatgttt
     1921 ataccattga tcgttcattt tttaacctct aatcatcgta aaatcttagc gagcgcttta
     1981 taataatagt atcgtacatt ggaaaagttc atgtatgtaa aatatttgaa ataatcatac
     2041 ataagcatta ctttgatttt catatacatt aatcaaagtt ctgggctcca caatgttata
     2101 ttgttagtag ttgactgaaa taaaatgcac ttgtaacaag catttttcaa ttatagtccg
     2161 gggccccaac atagagaatt tcaaaaaaga aattctacag gcaatgcagg ttggcggggc
     2221 cccaacacag aagctgacga aaagtcagct tacgataatg tgcaggttgg cggggcccca
     2281 acatagagaa attggatcta caatttctac aggcaatgca agttggggta caacgataaa
     2341 gaaatatttt ttctttatca cactatgtct cactcacttt ccaaaatact aaagtaacat
     2401 ctttagtata tcaaagaatt tttgctataa taagttataa ttatataaaa aaggaacggg
     2461 ataaaatgat tgtaaaaaca gaagaagaat tacaagcgtt aaaagaaatt ggatacatat
     2521 gcgctaaagt gcgcaataca atgcaagctg caaccaaacc aggtatcact acgaaagagc
     2581 ttgataatat tgcgaaagag ttatttgaag aatacggtgc tatttctgcg ccaattcatg
     2641 atgaaaattt tcctggtcaa acgtgtatta gtgtcaatga agaggtggca catgggattc
     2701 caagtaagcg tgtcattcgt gaaggagatt tagtaaatat tgatgtatcg gctttgaaga
     2761 atggctatta tgcagataca ggcatttcat ttgtcgttgg agaatcagat gatccaatga
     2821 aacaaaaagt atgtgacgta gcaacgatgg catttgagaa tgcaattgca aaagtaaaac
     2881 cgggtactaa gttaagtaac attggtaaag cggtgcataa tacagctaga caaaatgatt
     2941 tgaaagtcat taaaaactta acaggtcatg gtgttggttt atcattacat gaagcaccag
     3001 cacatgtact taattacttt gatccaaaag acaaaacatt attaactgaa ggtatggtat
     3061 tagctattga accgtttatc tcatcaaatg catcatttgt tacagaaggt aaaaatgaat
     3121 gggcttttga aacgagcgat aaaagttttg ttgctcaaat tgagcatacg gttatcgtga
     3181 ctaaggatgg tccgatttta acgacaaaga ttgaagaaga atagttcaac atatactaag
     3241 actaaagtat gaacatcatt tagttccgga gcctattcat attggtttcg gaactgtttt
     3301 ataataatta agaacacaat caatgcgtca tttcaaaaat atgttgtaac aaagtagttt
     3361 ttaagcaaac atatcatcga catcaacgaa gatacatagc gcatttggta ttttaaaact
     3421 tattataaaa ggtgatagtt atgaactatg ttgaacgtta tattgaacag tttttgagag
     3481 caacagtaag aaataatatc aagcactacc ttttaatgct agatg
//
LOCUS       DAMQYT010000218         3517 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMQYT010000218 DAMQYT010000000
VERSION     DAMQYT010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.222"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            47..883
                     /locus_tag="PGA33_002435"
     CDS             47..883
                     /locus_tag="PGA33_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474218.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIVLIVFAVVMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCFLLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(1144..2121)
                     /locus_tag="PGA33_002436"
     CDS             complement(1144..2121)
                     /locus_tag="PGA33_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDG6474219.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            2273..3310
                     /gene="mutY"
                     /locus_tag="PGA33_002437"
     CDS             2273..3310
                     /gene="mutY"
                     /locus_tag="PGA33_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDG6474220.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
BASE COUNT         1218 a          502 c          600 g         1197 t
ORIGIN      
        1 atttgaaaga agacaaatgg atttatattg aattgtaggt gtaaagatgc tagataatat
       61 tattttatat tttaaaaact taccgcatac taaaaggtat gtaacagaac gtttaaaaca
      121 aagttggaaa agttttctaa tcgtattagc tgcatgttta atacttatta ttgcaagtga
      181 aacgctattt tcctttagtc atttgaccga tgttaaagag gtgagatggt tatttagaat
      241 cattgtattg attgtttttg cggtagtgat gttcacaatt tatatctctt atcatcatta
      301 tatgaatgac tttttagtta ctaagttatt taatatttcc gcagcgacgc cagtagttat
      361 tatgtctatt ttaagtttta ttatgctagt cattttgacg atgattagtg cattggttaa
      421 accagtaaac ttcgaaacat catatatagc gctattctat tttattgtga tggcaactat
      481 ttttgtagga ttaatatccg ttacttttgg tcttattcga ctgttaactg aaaaaataaa
      541 tatcatattt tatggggttt gtgtattgtg tttcttattg ttaccaatta ttttcatacc
      601 aaatccaaat catgtattta tcaatcacat cctaatgcta aatccaatgt attacattgt
      661 taatggtata gcacaatcta tcatatttgg tatatcaagt atggaaaaca ttccatatca
      721 tttttacttt attttattct tatgtttaat agctgcagta aatttcgtat tagcaaggta
      781 tacgacacac gcaatttata ataaaacatc taaagtgaca caaactgata atcaacagga
      841 tgtttcaaat gatagtacag acgaggcgga tacttcttca tgatgtttag ctgacctgtt
      901 gtgtaaatta atctgtgaga atcagaaaca attagaatat catctagata ttgttttcct
      961 atactgattt tgatatttcg agtcattaaa ggtagtttgt tagcaacaat ataaatttta
     1021 gattttgtaa aagcattcat agttttacat taaaaaaccg gacagagtaa aaatcgtttt
     1081 gattttcatt ctgcccggtt gttcattttt tatagattaa ctttagtgaa taataacttg
     1141 aaattattta gcatacagtt tacgttgtaa cttatcttct gtaaataccc agccaatata
     1201 agagtggtct atgacattat cgtttgttac atgtgcaatt gccacaaatg aataatgatc
     1261 attttttaaa taacgcaaat caatcaaacg aatttcagta gaaccatctg ctaacgttgt
     1321 tgtttgccaa cgatagattg atgaaaagtt taaaaaagta cgtatatctt tattaccttt
     1381 gactttccat aaaatggagt ctgttgataa tgtttgacgt tccactttat cactaaacac
     1441 cacattccta ccatatgctt ttccaacata gtcatgtgca tcagtttgta tcgcgacacg
     1501 ccattccata aattttattg ttggcgcaac aaatacttta acagggtgat gctcttgttg
     1561 aattgctttt aaagcttgtt gcttaattac ggctctcatt ttaaaacgaa tcatgtaata
     1621 aatgattagt aaagcgatta taggaaagaa gactgcaaat ggatgcaagc ctataaccca
     1681 taatacaata ccaatacaaa gaactgtgaa aataataggg tcaaatgtgt taatcacact
     1741 taattgaatc catttgtttg tgataggtct aagcgcttgt gtaccataag aattgaatat
     1801 atctacaaag acatgtaaaa atactgcgag ctgagcccac atccatacat gaaatgggtt
     1861 ggttccacta aagaacgtga atattaaaaa tgtaattaaa attggccata gtattgtgaa
     1921 agggatggaa tgcgtgatac ctctatgatg cgaaatatat gttgcattgt cctttaattt
     1981 aagaacagta tccccatcag gaattaatga accaacgata agggttgtag ctgttgcacc
     2041 aaacgtagaa gccattgctg gatcttgagt tgcaagtgct gtaaggccca ccccaattgc
     2101 gatatgtgtt gcagtgtcca tatcagttca ctcatttcat aatattatat atactattat
     2161 aatccaattt cgcaaataac taaattaact tacatactaa ccatgatttt aatgtacaat
     2221 aaaaacatta tgagtaaaga caattaattt tggtgaggaa agggttttat aaatgtatca
     2281 gcagtctagt tttaaagaaa atttgataca ctggtttgat gaaaatcaaa gagaaatgcc
     2341 atggcgacaa acgaccaatc cgtactatat ttggttaagt gaggtcatgt tacaacagac
     2401 acaagttaaa acagttattg actattatca tcgttttgtt gaacgatttc caactgtgga
     2461 agttttgagt caagcttcgg aagatgaagt gttaaagtat tgggaaggct taggatatta
     2521 tagtagagct cgaaattttc atacggcgat taaagaagtt catgataaat atgaaggctt
     2581 agttcccaaa gatcctgatc aatttaaagc gttaaaaggt gttggcccat acacacaagc
     2641 tgcagtaatg agcattgcat ataatgtacc actagcaact gtagacggaa atgtttttcg
     2701 agtatggtct agattgaatg atgactatcg agatattaaa ttacaatcga cgaggaaatc
     2761 gtacgaacaa gaattattac cgtatgttac tacagaagct ggaacgttta atcaagctat
     2821 gatggaatta ggggcgttaa tttgtacacc aaaaaatcca ttatgcttat tttgtccagt
     2881 tcaagaaaac tgtgaggcat ttgataaagg tacatttgaa aagttacctg ttaaatctaa
     2941 aaatgtaagt aaaaaagtaa ttgagcaatc cgtcttttta attagaaata atcaaggtca
     3001 atatctgttg caaaagcgta gcgaaaaatt attgcatggt atgtggcaat ttcctatgtt
     3061 tgaaagtgag catgctagac gtaaaatgac tgaaaaaatc ggtcatgaca ttcaaccagt
     3121 agaaacacct atttttgagt tgaagcatca atttactcat ttaacatgga agattaaagt
     3181 atacgccgtt tcaggagcaa ttaacattga aacattacca gatgacatga tttggtttga
     3241 tttaagtgat cgagatcaat atacattccc tgtacctatg tcaaagattt atcaatttat
     3301 aaatgggtaa cattcgatga ttggtaatat tatcggaaaa catagtgaca ttaattttaa
     3361 aaagtataaa tgatatcttt aaatctcata gaataacaat gtcaaaaagt taaagtgttg
     3421 atatataaaa gtgatgattt tatatacttt aaatgggaca tgtaaatctc tgttgtagta
     3481 cttagcaata ttaccgagtt taattattaa gtagtaa
//
LOCUS       DAMQYT010000219         3499 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMQYT010000219 DAMQYT010000000
VERSION     DAMQYT010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.223"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..273
                     /locus_tag="PGA33_002438"
     CDS             <1..273
                     /locus_tag="PGA33_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDG6474221.1"
                     /translation="TYWHDAHKWNDRAKAEGYKVDKNPEPGALFIAEQGAGGHDGHYG
                     HVAVVIGVSDGGKTFRITEMNWEGAFKVNERTLKMTDGYSFIHDKE"
     gene            280..870
                     /locus_tag="PGA33_002439"
     CDS             280..870
                     /locus_tag="PGA33_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474222.1"
                     /translation="MKQKIIMFTLVTVILFCAVLIGYQIPKQQVKMKQNQIEDLQEEQ
                     RILRDKNGELNKLVKRQSKTVISDEEKQIREVSSNFVKQMFEMKKDSSFKSKAPQIKP
                     LVTKDYYDTLFKDSKDKYDLYDDITVNDIHVYFDTYDPKKDSYKVFVQFDERIETDGD
                     DKIEHRQTSAQLDLVRTAEGWRIDNLKRFNLKPLGR"
     gene            926..1282
                     /locus_tag="PGA33_002440"
     CDS             926..1282
                     /locus_tag="PGA33_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin-like fold lipoprotein"
                     /protein_id="HDG6474223.1"
                     /translation="MRRWFVLILGLVILLSACGQKYDKEIDEVTKLEKESIQDVKNTK
                     KYKNVERSKSYYKIYNDGEVIIMTYMPFKDSNTKVSRVYKINQTSDKYEEDSNIDAEK
                     FEKDNKPVYEENNMKK"
     gene            1391..1894
                     /locus_tag="PGA33_002441"
     CDS             1391..1894
                     /locus_tag="PGA33_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDG6474224.1"
                     /translation="MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYR
                     EVNKRSVDGVYNPLLAEEDAIKSRSISDEQVAKRRRLNDETKAYIEEKLSLKWSPRQI
                     ASQIEKDTGYYISYPTIYRYIRNGLVKVDVKRDMRQAGKKYNKSSEKRGKLDVGSRVI
                     KYRLKKY"
     gene            1875..2411
                     /locus_tag="PGA33_002442"
     CDS             1875..2411
                     /locus_tag="PGA33_002442"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDG6474225.1"
                     /translation="MGSKSINNREEFGHYEIDTVWSVRPSTYCLLTIIERKTRYLYAS
                     RLNTRKSNVVCNEIINIMKPLLPKSITMDRGKEFALFEEIEDELNCSIYFSDLGCPYQ
                     RGSIENANGLLRQYYPKGTDFANITQKSLDEAVKQINARPRMIFDYKSSEEMLKYHVS
                     TQNCEPILNDCVRHEPVN"
     gene            complement(2632..3147)
                     /locus_tag="PGA33_002443"
                     /pseudo
     CDS             complement(2632..3147)
                     /locus_tag="PGA33_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
BASE COUNT         1291 a          423 c          599 g         1186 t
ORIGIN      
        1 acttattggc atgatgcaca taaatggaat gatagagcta aagctgaggg ttataaagtt
       61 gataaaaatc cggaacctgg tgcattattt attgctgaac aaggtgcagg cggtcatgat
      121 gggcattatg gtcatgtggc tgttgttatt ggtgtaagtg atggtggtaa aacatttaga
      181 ataacagaaa tgaattggga aggtgctttt aaagttaatg aacgtacatt gaaaatgact
      241 gacggatata gttttattca tgataaggag tgaaaataaa tgaagcaaaa aattattatg
      301 tttactcttg taacagtaat attattttgt gctgttttaa ttggttatca gattcctaaa
      361 caacaagtta aaatgaaaca aaatcaaatt gaagatttgc aggaagaaca acgtatatta
      421 agagataaaa atggagagtt aaataaatta gtaaagagac aatcaaaaac agttatttct
      481 gatgaagaaa aacaaatacg tgaagtttct tcaaattttg ttaaacagat gtttgaaatg
      541 aaaaaagatt cttcttttaa atcaaaagca ccacaaataa agcctttagt tacaaaggat
      601 tattacgata cattatttaa ggattctaaa gataaatatg atttatatga tgatataacg
      661 gttaatgata ttcatgttta ttttgatact tatgacccta aaaaagatag ttataaagtt
      721 tttgttcagt tcgacgaacg tattgaaaca gatggagatg ataaaattga acatcgtcaa
      781 acatctgcac aattggattt agttcgtaca gctgaaggtt ggcgtattga taatttaaaa
      841 cgtttcaatt taaaacctct aggacgttag tttgtttcaa gacgatagca atgatataat
      901 ttacgtaaaa aggagtgaat ttatcatgag gcgatggttt gtattaattt taggattagt
      961 tatattatta tctgcatgtg gtcagaaata tgataaagaa attgatgaag ttacaaagtt
     1021 agaaaaagaa agtattcaag atgtgaaaaa tacaaagaag tataaaaatg ttgagcggtc
     1081 aaaaagttat tataagattt ataatgatgg tgaggttatt ataatgactt atatgccttt
     1141 taaagatagt aatacaaaag tttcaagagt atataaaata aatcagacta gcgataaata
     1201 tgaagaagat tctaatattg atgctgaaaa gtttgagaaa gataataaac cagtttatga
     1261 agaaaataat atgaaaaaat aaagaatcaa ctccctatac gctttagcga tggtatcggg
     1321 agttgatgaa catatatcct cttataactt attttataaa taaccttttt aaaagtaaag
     1381 gaggatatac atgaaaaaaa gaaaaaaacg taaagaatta gactataaaa cacgtctagc
     1441 tatcgcaaag tatttaaatg atggtttaaa catcattcag atttctaaga ttttaggtcg
     1501 tgatgatgca gttctttata gagaagtaaa taagcgttct gttgatggtg tttataatcc
     1561 tttattagct gaggaagatg ctataaaatc gcgtagtatt tcagatgaac aagttgctaa
     1621 acgtagacgt ttgaatgatg aaacaaaagc atatattgaa gaaaaacttt cattaaaatg
     1681 gtctcctaga cagatagcaa gtcaaattga aaaagataca gggtattata taagttatcc
     1741 tactatatat agatatattc gcaatggatt ggtaaaagtt gatgttaagc gtgatatgag
     1801 acaagcaggc aagaaatata ataagtcttc tgaaaagcgt ggcaaattag acgtagggtc
     1861 tcgtgttata aagtatcggc tcaaaaagta ttaataatcg tgaagaattt ggacattatg
     1921 aaattgatac tgtttggagt gttagaccat caacatattg cttgttaact attattgaaa
     1981 gaaagacaag atatttatat gctagtagat taaatacacg taaatccaat gtagtttgta
     2041 atgagattat aaatattatg aaaccattat tacccaaaag tataactatg gatagaggta
     2101 aggagtttgc tctatttgaa gaaatagaag atgaattgaa ttgtagtatt tattttagtg
     2161 atttgggttg tccatatcaa cgtggttcta ttgaaaatgc aaatggactt ttaagacagt
     2221 attatcctaa aggtacggat tttgcaaata taacacaaaa gtcattagat gaagctgtta
     2281 aacaaattaa tgcacggcct cgaatgatat ttgactataa atcgagtgag gaaatgctaa
     2341 agtaccatgt aagcacacaa aattgtgaac ctatattaaa tgactgtgtg aggcacgaac
     2401 ccgtcaatta atataggtta atctaagcga atggtttaca atgagcgcgg aaggacagga
     2461 cgaataaatt ttgtatgtaa aatgcataat ctaaaaagaa gcatactatt taaatctgtg
     2521 gtttttgaca gatattacta gtaaaatgct tctttttctt cttctgtact cttttagtaa
     2581 tatttattaa aaaaatttaa gaaattctca tttttctatt gacacactgc ctattttaaa
     2641 gtccagttcc ttcaatgata cttcttcctt ttgaataaaa aacaactcgt ttatcgaaaa
     2701 gccattttta gcatcaactt ttttaacttg tagacttgga ttacttacaa atccaaacac
     2761 tgatctcaca tttttctttg ttataccgcc aacgctatat atgccacctt ttatatctat
     2821 taattcaggt actgcaaaga catcaaggcc atgtgttttt tctttatatt tatttttatc
     2881 gtcaccaagt aaaaatactt taaaagcttt atttcgttgt ttaaagttta aaacgttaga
     2941 gccatagtaa tcttcgcttt taacactaat gtttgttggt tcaaaacttt cttcagaata
     3001 gtattgatgt agcatattga tataatattt gcgttgtgtc tcatcaagtt ctactttcgc
     3061 gtgaccagtt tgactttctg ctgtaaacac acctgtagtt aatattccta atgctaatgt
     3121 tgctttagct atcgttgtta atttcatatt tttttgctcc aatcttaatg tattggattg
     3181 ttattattac gtaatttgaa ctatctgtct ttattataag gtgcatcaaa acagactgac
     3241 attaacgctc atttaaataa acttgaatca ttattaatga aaatcaaacg tattttaaca
     3301 ttcgtattaa ctgattttta atagcaacat aaatattttt ttggtgaatc attgttcttt
     3361 ccgattagtt ttaaatctta agctattggt tataaagtgt acaaaaaagc agcttaataa
     3421 cgtagtcaat gttcgatatg tttttattat aaaaacacgt atcaaaatca ttaaacatgg
     3481 gattattaaa ccgcttctt
//
LOCUS       DAMQYT010000220         3422 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMQYT010000220 DAMQYT010000000
VERSION     DAMQYT010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.224"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            80..346
                     /locus_tag="PGA33_002444"
     CDS             80..346
                     /locus_tag="PGA33_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474226.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            490..798
                     /locus_tag="PGA33_002445"
     CDS             490..798
                     /locus_tag="PGA33_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474227.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISFINQTAIADKHK
                     NQIAYIFTLCISLSAVFLMSSILSFEYDFIALAFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(906..1139)
                     /locus_tag="PGA33_002446"
     CDS             complement(906..1139)
                     /locus_tag="PGA33_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474228.1"
                     /translation="MLRSLCKHYRILINAIKVGIEMKYKISLAYNLAIIIGSLIILCI
                     LISRGYDIYVILIPILTILASLINLFCDIKKHK"
     gene            complement(1120..2313)
                     /locus_tag="PGA33_002447"
     CDS             complement(1120..2313)
                     /locus_tag="PGA33_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDG6474229.1"
                     /translation="MLYLEVLKNRNFTYLLIGNFLRRSCFVLFSLQIIWFTVELTNQS
                     SLKLSMMVMSQTLPFIIFGIFGGAYSDKHNKKKILYLSDLILSLIIIIIPLLAITSNL
                     NYLTLLTISTAITIINCYTDPAFRAILPEIIDEEHLATSNALIDSLQRGSNIILPALI
                     GVIVILVGNVGVFFICSILLFLGFIFNALLKYTNNNMIDRHSKEDFSETWEFLKQSKE
                     IPFIIIIQFACILINTGLWRVVLPLFISNILKEGVGVYGLATSCLGIASLLMSLIMGL
                     LSEKRLIFKFSIGVLVWGIGLSIINVFPSVAILYIGATLLGLGQSIEGLTRSVAIQIK
                     TPNHLMGKVFSISSTSNYAADTLSLGLISIIIPLLSLSNIFILGGVIISILSLAGLKF
                     VKKSM"
     gene            complement(2697..>3422)
                     /locus_tag="PGA33_002448"
     CDS             complement(2697..>3422)
                     /locus_tag="PGA33_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E domain-containing protein"
                     /protein_id="HDG6474230.1"
                     /translation="VNELTEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTG
                     KVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTIT
                     TPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSG
                     PVNPNNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKTGLESTQ
                     KGLIFSSIIGIAGLMLLARRRKN"
BASE COUNT         1153 a          592 c          447 g         1230 t
ORIGIN      
        1 gcgatgaaaa aggtttagaa ttacaaaaat aactatttta aatttagtta caaatatatt
       61 ttagaaagga tgtgaaataa tgaaaggctc taaacaaata cttttgatta tgggcattat
      121 atctcttatt gttttattta tttttacact attcatcatg gcgcaatatg caaaacatta
      181 tgaacaaaaa tccgacagtt ccaacgcaca tactttaaat tcatcatcag ccataattga
      241 gcaacatact atgtcgaatt tagcgtctct agatttatac gctcctgttc gtaacattac
      301 ttctagtcgt gatattcatc ccatttattt catgacaaaa gattaaaact tcctttttca
      361 taaccccatt cattcaacta ataaattaaa aaatgataaa aacgtactgt tttttagaaa
      421 catacatata taagaaacgc atccattact aaaagacagt acattatatt ctatcaggaa
      481 ggtgcaacaa tgaccgtcaa catcgtattt agtatcgtat tctgcataag tatggttata
      541 ttaggcattt atgtcgcaat aactaaagat ttcacactaa tctcttttat aaatcaaacg
      601 gccattgcag ataaacacaa gaaccaaatt gcatatatat ttacgctatg tataagtttg
      661 agtgcagtat ttttaatgtc ttcaattcta agctttgaat atgattttat tgcattagca
      721 ttcttatttt taacgatagc attattactt atcgcattat tctacgtttg cttttataaa
      781 attacaaaat acccataaat gtttgtgata gtgtgttggt tagatttatt gatgcgctgt
      841 cagtaagtaa ttgtttgcga aaaaaataca accttgtacg aatacaaagt tgcattaata
      901 tagaatcatt tatgcttttt aatatcacaa aacaaattga ttaaactagc taaaatagtc
      961 aaaattggaa tcaagattac ataaatatca taacctctag atattagtat acataatata
     1021 attagcgacc ctattataat tgctaaatta taagccaaac taattttata tttcatttca
     1081 attcccacct taatagcatt tattaaaatt ctataatgtt tacatagact tcttaacaaa
     1141 ctttaaccca gctaagctaa gtattgaaat aattacacct cctaatataa atatattact
     1201 taagcttagt aaaggtataa ttatacttat cagtcctaaa gacaatgtat ccgctgcata
     1261 attcgatgta gatgagatac taaatacttt ccccatcaaa tgatttggcg tttttatttg
     1321 aatggcaact gatcttgtta gtccctctat agattgtcca agtcccaata atgttgcacc
     1381 tatatataat atcgccacac ttggaaacac attaataatc gataagccaa ttccccaaac
     1441 taaaacacca atactaaatt taaagattaa tcgcttttct gacagcaaac ccataatcaa
     1501 tgacattaat aaagatgcta tacctaaaca tgatgtagct agtccatata caccaacgcc
     1561 ctcttttaat atattggaaa taaacaatgg taatacaaca cgccaaaggc cagtattaat
     1621 caatatgcag gcaaattgga tgattataat aaatggaatt tctttagatt gtttcaagaa
     1681 ttcccaagtt tcagaaaaat cttcttttga gtgtctatca atcatgttgt tatttgtata
     1741 ttttaaaagt gcattaaaaa taaatcctaa aaatagcaat atactacaaa taaaaaagac
     1801 gccaacatta ccaactagta ttacaatgac accaattaaa gcaggtaaaa taatatttga
     1861 gcctctttgc aaactatcga ttaacgcatt acttgttgct aaatgctcct catcaataat
     1921 ttcaggaaga attgccctaa acgcaggatc cgtatagcag ttaataatgg tgatagctgt
     1981 agatatggtt agaagcgtca gataatttaa atttgatgtt attgcaagta aaggaataat
     2041 tattataatc aaacttagta taagatcaga tagataaagt attttctttt tattatgttt
     2101 atcagaatat gcgccaccga aaataccaaa tataataaat ggaagtgttt gactcataac
     2161 catcattgat aattttaaag atgattggtt tgtcaattca acagtaaacc aaattatttg
     2221 taacgaaaac agcacaaaac aactccgacg taagaaatta ccaatcaata aatatgtaaa
     2281 gtttctattt ttcaaaactt ctaaatacaa catatttatc acctctcata aaaataattg
     2341 aatgcatcca ccagcttttt tagaccttct tctaaactct ctttatccaa agcgcaatta
     2401 attctaatat aatttagtca gttaaatatc aattatttcg aaatatacat actacttgaa
     2461 acaccataca taacccccaa aatgactact cagaggttat attctactaa ttatgattat
     2521 attaaatatg aaaatattat caaaaaaatc aaatttataa caaaaataca ccccttaaag
     2581 ttaggtcttt caatccaact tttggggtgt atatcattct catcatattc taggttgttt
     2641 ttaacaaact aaatatagtg aatgcaaatc aactattatt taaattatga attattttaa
     2701 ttctttcttc tacgagccaa taacattaat ccagcaattc caattatact actaaagatc
     2761 aaaccttttt gcgtgctttc taaacctgtt tttggtaatt ctgctcgttt tttctcttga
     2821 ttagctactg attctttagc aattttagat tttttaactt tatcattttt atccattgaa
     2881 tgaactgggc catttggttt tgctctgtct ttcgataatc ctggattgtt aggatttact
     2941 gggccacttg gatgagttgg tctgctcggc ttctctgggt tttcaggtcc ttttggatct
     3001 tttggtttct ctccaccgaa ctctacaatc ttatctactg gttgttttgt gatctcttct
     3061 gttggttgac cctcgccaac tttttcacct gttaatgggt tcactgtgat tggtgttgtg
     3121 attgtcttac ttcctggttg tccttcttgt ttcactcgct cttcaccagg ttgtaatttt
     3181 ggattaaact cacgttttgt ttcaaacggt atctctactg tttttgtttc tggtgtaccc
     3241 gtttttggtc cgtgtttaat cacatcatcc actggctctt cgatcacttt tcctgtgtct
     3301 ggattcttga ttcctggttt acctggtact ttttccgttt gatctgtggg taagtttgga
     3361 tcaaagatat ctttatgacc ttgcggtatt ttctcgccac cgaattctgt taattcatta
     3421 ac
//
LOCUS       DAMQYT010000221         3384 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMQYT010000221 DAMQYT010000000
VERSION     DAMQYT010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.225"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(128..1483)
                     /locus_tag="PGA33_002449"
     CDS             complement(128..1483)
                     /locus_tag="PGA33_002449"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDG6474231.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIALDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            complement(1510..2442)
                     /locus_tag="PGA33_002450"
     CDS             complement(1510..2442)
                     /locus_tag="PGA33_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HDG6474232.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            complement(2444..3253)
                     /locus_tag="PGA33_002451"
     CDS             complement(2444..3253)
                     /locus_tag="PGA33_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDG6474233.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
BASE COUNT         1025 a          667 c          427 g         1265 t
ORIGIN      
        1 tattttatat tacatattga ttactttgtt tttattttgt ttatatcatt ttacgtttgt
       61 actataaatt atttctacaa acacaaaaaa ccgatgcata cgcatcggct catttgtaat
      121 acagtattta tttatctaat cccattttat cttgaaccac atcagctatt tgttgtgcaa
      181 atctttcagc atcttcatca gttgctgctt caaccatgac acgaactaat ggttctgttc
      241 cagaaggtct tactaaaatt cgaccttctc cattcatttc tacttctact ttagtcataa
      301 cttctttaac gtcaacattt tcttcaacac gatatttatc tgttacgcgt acgttaatta
      361 atgattgtgg atattttttc atttgtccag ctaattcact tagtgattta ccagtcattt
      421 ttattacaga agctaattga ataccagtta ataaaccatc accagttgta ttgtaatcca
      481 tcataacgat atgtccagat tgttctccac ctaagttata attaccgcga cgcatttctt
      541 ctactacata tctgtcgcca actttagttt tattagattt aattccttct tgttcaagcg
      601 ctttgtaaaa acctaaatta ctcataacag tagaaacaat catgtcatta ttcaattctt
      661 gatttttatg catttcttga ccaataataa acataatttg gtcaccgtca acgatttgac
      721 cattctcatc tactgctatg attctgtctc catcgccgtc aaatgctaac ccaaaatcac
      781 tttcagtttc aactactttt tcagctaatt tttcaggatg tgtagagcca catttctcat
      841 tgatattata tccatcagga ctacatccaa ttgtttcagt atctgcttct aagtcaccaa
      901 ataagaatgg cgctagtgat gatgttgaac catttgcacc atctaaagca attttcaaac
      961 cttcaaagtt aacatctact gttgatttta aatagctcaa atatttttgt gccccttcaa
     1021 agtaatctga ataatgtaca atatcattgc caactggtct tggtaattct gggttttctt
     1081 gatccaataa tgcttcaatt tcattttctt gttcatctga tagtttaaaa ccatctgatc
     1141 caaagaattt aataccatta tctgcaactg gattatgaga ggctgaaatc attacaccta
     1201 actctgcacc catatcgcgt gttaaatatg caacacctgg tgttgaaata atacctaatc
     1261 gcatcacttc tgcaccaatt gaaatcaaac cagctattaa tgctgattct aacatttcac
     1321 ctgaaactct agtatcgcga cctacaagta cacgtgggtg tttttcacct ttattatgtg
     1381 ctagaacata gccaccgtat cttcctaatt taaatgccaa ttcaggtgtt agttcttggt
     1441 ttgcgacacc tcttactccg tctgtaccaa aatattttcc cattgtttac tctcctttaa
     1501 tttagctatt tattttacat ttatataagc cttcgtttca cttggttgtg ctttagtgac
     1561 atgttctggt aaattgattt ttacagtctt ttcagttgat tctgaaatac catctaaatc
     1621 tacttctgca tcaacttcgc ttatattttg taagtcatct cgactaccga agatttcaat
     1681 ttctttatct tctaaatcaa tcgaacttaa ctctttatca tctgctaaac tacctttctg
     1741 tttaacattt actttaacct ttttgctaaa aggctctact ttaacttgta aattcacttc
     1801 attaggttga atcgatacat ttaatttatt cagtttttta tcaaaagccg ttacttctgc
     1861 gacatctttt gtgtcaccat taatcttttt attagtttta aaagtggctt ttaaataagc
     1921 gatatcattc aattgttctt ctccacctgt tactttaact gtttgtggtg aaacttcttg
     1981 ctttgtaatt ttataaagtg gatcaatatc actctgactt acatcaggtt gaactgtcat
     2041 ctttttagtt actttgtttt caagcgtaat atttgctaat ttaggtttta cagaataatg
     2101 aatgtcatca gctaaccctt taacttgata cttctcttga tattttccag ctttagcatt
     2161 tgataaatca atcactactc ttaaatcttc tggattttca atttttatta tctttgattg
     2221 tggtcctgaa atagtcacat taactgtttc aggcgctttt gttaaatgca aatctttagt
     2281 gttataaaga atttcaacgg gtacatcttg aatcgtttta ctagactttt gaccaagatt
     2341 accagtgtta aagatatttc caaaaacatt gttaacagat aaaaagaaaa acaatgccaa
     2401 aagaaaggca ataaatctca agccccattt actttctagc atattatttc acacctttct
     2461 tttgaaagcg tgtgccaaac caatgttcag caagcaactc ttcaaaaatt tcgtttgaaa
     2521 tgtctcgtcg taattttcca tcaaatgtta ccgaaatatc accagtttct tcagatacaa
     2581 taacggtaaa tgcatcagat acttctgaaa taccaaccgc agctctatgt cttgtaccca
     2641 aacttttaga tatcttagga ctatcagaca atggcaaata acttgctgct gctgcaatct
     2701 tcgtgccttg aataatcatt gcaccatcat gtaaaggtgt gttaggtata aagacattaa
     2761 ttaaaagttc ttgcgaaata tttgaatcca ttgcaatacc tgtttcaata taatcttgaa
     2821 gacctgtttc tttttcaaag acaattaatg cacctatacg tcttttagcc atatattgca
     2881 cagcctttga aaccgattga atcaatttct cttcatcttt actatacgta ttagaagtat
     2941 agcgttttaa aaagctacct ctaccaagtt gttctaacgc acgtctaatt tctggttgga
     3001 atattactat taaagctaat accccccatt gaataacgat atcgaataat ttagatgttg
     3061 cagtcaagtt caatatcata cttatctgct gaccaataac aattactaat atccctttaa
     3121 gtaattgtat cgctttagtt cccttaaaga ccgtgatgag aagataaagt acataccaaa
     3181 ctatcagtaa atcaaggata ctcgttacaa tttttaacgt actgaggttt tgaaaaaagt
     3241 tggaaaaatc cataacatct cctccgggta atatttttcc ataataccca ttataccaat
     3301 catttacatc actgtcatac gaacatatat ttaaatagaa aaaatttatt ttaaagatta
     3361 taactactct taatcatttt agtg
//
LOCUS       DAMQYT010000222         3356 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMQYT010000222 DAMQYT010000000
VERSION     DAMQYT010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.226"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..363
                     /locus_tag="PGA33_002452"
     CDS             <1..363
                     /locus_tag="PGA33_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HDG6474234.1"
                     /translation="VLAIILFIATYLNISKNQGRSPFFKPLHMILRLFMLLTLISGFW
                     ILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWITIALIIITM
                     VLGVILPLGPISKLFGIG"
     gene            complement(534..1850)
                     /locus_tag="PGA33_002453"
     CDS             complement(534..1850)
                     /locus_tag="PGA33_002453"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDG6474235.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRRYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVSVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEINAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(1902..2726)
                     /locus_tag="PGA33_002454"
     CDS             complement(1902..2726)
                     /locus_tag="PGA33_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDG6474236.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            2840..3148
                     /locus_tag="PGA33_002455"
     CDS             2840..3148
                     /locus_tag="PGA33_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDG6474237.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
BASE COUNT         1043 a          548 c          566 g         1199 t
ORIGIN      
        1 gtattagcga ttattttatt tatcgctaca tacttaaaca tttcaaaaaa tcaaggcaga
       61 tcaccatttt tcaaaccgtt gcacatgatt ttacgcttat ttatgctgtt gacgttaatt
      121 tcaggatttt ggatattaat tcagtcattt atgaatggcg gggcaaatca tatgttgctt
      181 acattgaaaa tgctgtgtgg tgttgcagta gttggattga tggaagtgtc gattgctaaa
      241 agaaagagac atgaacaaag tcacacaatg ttttggataa caattgcatt aattatcatc
      301 acaatggtat taggtgtcat tctaccgtta gggcctatat caaaattatt cggtattggc
      361 taattgataa gacatatagt atatgacata aggcatggac aattattagt ccaagacctt
      421 atgtcttttt tgattctaaa aatgttaaac ataaaaaatg gactggatag tagtaccagt
      481 ccaacataat tctaacctag acaattaaga ttagattgaa tcgcatgatt catttattta
      541 gctttgtaac caatcatatt gattaaatct ttagggtggc tatatggtgg tgcataagcc
      601 acttcaaact cagttaactc atctacagtt agctggttca tcattgccat cgatagtaca
      661 tcaatacgtt tatctgcacc ttcttttcct actgcagctg ctcttaaaat ctgacggttt
      721 gaagtgtcat aatatactct taagtgtaaa ggggaatttc ctgggtaata attcgcgtgt
      781 gcaccttgag tgacttctac cattttatag tcaaattgct ttagttcgtt tggtttaacg
      841 ccgacactcg caaatgtata atcaaagaac ttcacaatat tgttgcctaa gaagcctttg
      901 aattcaatag tgtcatttcc agcaatttgt tcggcaacaa tacttgctgc acggtgagcg
      961 ccccaagcta aaggaacact agccggtaga tcgacatgtc gataatgtga tgttgcaata
     1021 tcgcctattg cataaatgtt tggaacattt gtttcaaatt tatcgtttac cggtatgaaa
     1081 ccttttcgat caagtttgat atttgaactt tcgataaatt ttgaattggg gtgagtaccg
     1141 acaccttcaa taatcatatc gtaatgttca acttttcctg atttaaatgt aatttcattt
     1201 ccattgatag cattaatttc ctcatttaaa cggtatggaa tctcccgctt atctaattca
     1261 tcaagtatag gttgattcat gtcggcatcc attaatttat ttatcttatc agatcgatga
     1321 attaaagtag ggtgtaaacc acgttcataa agattttcaa gaacttctaa tgaaacataa
     1381 cctgcaccta caaccaatac tttatcaact tgatttgctt tgatgaattg atcgatagca
     1441 tcagtgtctt ctaaatttct aagtgtaaat gtaatatcac tttcaaagcc aaggctattt
     1501 gcacttgcac cagggcttaa aatgagttta tcgtaagatt cttcaaattg ttcgtttgtc
     1561 tttctattta atacagatac agtttgtctt tcatcattga ttgcaataac ttcatgataa
     1621 gtttttactg taatttgctt tctatcataa aatttttcag gtgtatacgc taaagcatat
     1681 cttctatctt caacaacttc gccaatgaca taaggcaatg cacaattagc aaagctcata
     1741 tcacgatctt tttcaaaaat aataatgtca ctttctttat ctaaacgtcg aatttggctg
     1801 gcacatgttg caccgccagc gactgctccg actacgacta ttttgggcat cgtaacttac
     1861 ctcctattgt tttaaaatat tataaaatga ttagttataa attaacagta atatctaata
     1921 tttaaattaa agaaatcatt caaatatcga ccaatgccat cttcattatt gttgaatgta
     1981 atattgttcg ctacatcttt aagttcttgc aaaccatttt ccatagcaac accgtgacgc
     2041 gcgtactcaa tcatttcaat atcattatct tcatcaccga atgcaataat attatttcgg
     2101 tcaatattta aaaattgtct aacttgctca atgcctcttg ctttattaat accaagtttt
     2161 acaatttcaa tgacagggaa tggtgcgccc cagcgtcgat gctcaatatg atcggcataa
     2221 aaatgagtaa gcatattttt gatttcaggt attttacttt cttcggcttc aattaaaatt
     2281 gaggtagggg attctttcaa gtggacaagt aaattaccag tttgaattct tggattaccc
     2341 attgaaaaac cttcaaataa tcttggatca tgattgttaa tgaaaacata atctttcact
     2401 tctgctataa tattcgatac ttgatattgt tgtaatcctt gaataatgtt ttgtgcgatg
     2461 cctaaatcta aaatttcatg gcaagttttg aagtttttat ctttagggtg atgtacgtaa
     2521 gcgccattaa aattaacaat tggtgtcgtt aaatttaatt catgataata catttgactt
     2581 gcacgataag gtctgccagt cgcaatcata atttggtgtc cacgttgttg taattcattt
     2641 aatacttgtt tagtatatga tgaaatttct ttgttatcgt ttaataatgt tccgtctaag
     2701 tctagacata ttaaatgtgg ttgcatatct ctctcctcat aagttgctta gttcatatag
     2761 atacatcata gcattttatt ggcgttttgt cggatatttg atatattatg aataatatga
     2821 cattaaagag gtgatatgga tggaagaggc attgaaagat agtatcttag gtgcattaga
     2881 aatggtaatt gaccctgaat taggaattga tatcgttaat ttgggtttag tatacaaagt
     2941 gaatgttgat gatgaaggcg tatgtacagt tgatatgact ttaacatcaa tgggatgtcc
     3001 aatgggacct caaattattg atcaagttaa aacagtatta gcagagattc ctgaaattca
     3061 ggatactgaa gtgaatatcg tatggagtcc accttggaca aaagatatga tgtcacgtta
     3121 cgctaagatt gcacttggtg tgagctaaca taaacttgca ttgcctgtag aatttctttt
     3181 cgaaattctc tgtgtttggg cccgcagtca atataacatt gtagagtata attcattgtg
     3241 aataagctcc cttcaaagta gacattgaaa aatgaaaatt ttgaagggag ccttatttat
     3301 gttctagttt ctgattttat tttgaaaatt tatatctaac ttgtttcaaa aaatat
//
LOCUS       DAMQYT010000223         3287 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMQYT010000223 DAMQYT010000000
VERSION     DAMQYT010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.228"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            98..1231
                     /locus_tag="PGA33_002456"
     CDS             98..1231
                     /locus_tag="PGA33_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDG6474238.1"
                     /translation="MINEQRLLNTFFELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAVVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARHSK"
     gene            1299..2705
                     /gene="gndA"
                     /locus_tag="PGA33_002457"
     CDS             1299..2705
                     /gene="gndA"
                     /locus_tag="PGA33_002457"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDG6474239.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            2924..>3287
                     /locus_tag="PGA33_002458"
     CDS             2924..>3287
                     /locus_tag="PGA33_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDG6474240.1"
                     /translation="MEKLNQVMLYVDDVEKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKHSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAV"
BASE COUNT         1167 a          454 c          668 g          998 t
ORIGIN      
        1 cctgataaat ctgtgtaacg tcaattactg taacatttta tttcatcaat ttttaccgtt
       61 tggcgtacat gaaaattttg ataaaagggg ttccattatg attaatgagc aaagattact
      121 taatacgttt tttgaacttg tacaaatcga ttctgagaca gggaatgaat caacaatcca
      181 acctatttta aaagaaaaat tcatagcatt agggttagat gttaaagaag atgaagcggc
      241 taaacatcct aaattaggtg ccaataatct agtgtgtaca atgaatagta ctatcgaaga
      301 aggcgaagta cctaaattat atttgacgag ccatatggat actgttgtac ccgcaattaa
      361 tgtaaagcca attgtaaaag atgacggcta catatactct gatggtacga ctattctagg
      421 tgcagatgat aaagcaggat tagcagcgat gcttgaagtt ttgcaagtga taaaggaaca
      481 acaaatccca catggacaaa ttcaatttgt gattactgtt ggagaagaat caggtttaat
      541 tggtgctaaa gaattaaatt cagagttgtt ggacgcggac tttggttatg ctattgatgc
      601 aagtgctgat gtcggtacta cggttgtagg tgcaccgacg caaatgttaa tttcagctaa
      661 aatttttggc aaaacggctc atgcaagtac gccaaaagag ggtgttagtg ctattaatat
      721 tgcggcgaaa gcaattagtc gaatgaaatt aggacaagtt gatgaaataa ctactgccaa
      781 tattggtaag tttcatggcg gttcagctac gaatattgtt gcagatgaag tcattttaga
      841 agcagaggca cgttctcacg atccagaaag aataaaaaca caagtcaaac atatgactga
      901 tgtgtttgaa actactgcat cagaattagg tgggaaagca gtagttacag tggagcaaag
      961 ttatccagga tttaaaatta atgataatga agcggttgtt aaaattgcac aagaaagtgc
     1021 tcgtaattta ggattatctg caaatacaat tatttctggt ggcggttcag acggcagtat
     1081 tattaataca tttggtattc cttcagtcat tttaggagtg ggttatgaaa aaatccatac
     1141 aacaaatgag agaatgccga ttaaatcatt gaacttgctt gcaagtcaag tgttagaaat
     1201 aatcaaaata gttgcgcgac attcgaaatg atttagtgta gtgtatgtct attttgtgat
     1261 acaatactaa tgaaaaatca acaagtgagg taagtaatat gacacaacaa attggagtta
     1321 ttggtttagc cgttatgggt aaaaacctag cttggaatat tgaatcacgc ggatatagtg
     1381 tatctgtatt taaccgctca agtgaaaaaa ctgatttaat ggttgaagaa tcaaaaggga
     1441 aaaatattca tccaacgtat tcattggaag aatttgttaa ttctttagaa aaaccacgta
     1501 aaattttatt aatggttcaa gcaggtaaag ctacggacgc aacgattgat agtttgttac
     1561 ctttattaga tgatggagat attttaattg atggtggtaa tacaaactat caagatacta
     1621 tcagacgtaa taaagcatta gcacaaagtg caatcaactt tattggtatg ggcgtttctg
     1681 gtggtgaaat tggagcgtta actggtccgt cattaatgcc gggtggacaa gaagaagctt
     1741 ataacaaagt tgcagatatt cttgatgcaa ttgctgcaaa ggcaaaagac ggtgcttcat
     1801 gtgttactta tattggacct aatggtgctg gacactatgt gaaaatggta cacaatggta
     1861 tcgaatatgc tgacatgcaa ttaattgcag aaagttatgc tatgatgaaa gaattattag
     1921 gcatgtctca tgaagacatt gctcaaacgt tcaaagactg gaatgctggt gaattagaaa
     1981 gttatttaat tgaaatcact ggtgatattt tcatgaaatt agatgaaaat aaagaagcat
     2041 tagttgaaaa aattcttgat actgcaggtc aaaaaggtac aggaaaatgg acttcaatta
     2101 acgcattaga attaggtatt ccattaacaa tcattactga atctgtattt gcacgtttta
     2161 tctcttcaat caaagaagag cgcgtaaatg cttctaaaga attaaatggt ccaaaagcat
     2221 catttgatgg tgacaagaaa gactttttag aaaaaattcg taaagcatta tatatgagta
     2281 aaatttgttc ttatgcacaa ggtttcgcgc aaatgcgtaa agcaagtgaa gataatgaat
     2341 ggaatttaaa acttggtgat ttagctatga tttggagaga aggttgcatt atccgtgcac
     2401 aattcttaca aaagatcaaa gatgcttacg ataataatcc aggattacaa aacttattgc
     2461 tagatcctta ctttaaaaac atcgttactg aatatcaaga tgcgttacgt gatgttgtcg
     2521 caactggtgt tcaaaatggt gtaccaacac ctggtttctc atcaagtatt aattactatg
     2581 atagttaccg tgctgctgat ttacctgcaa acttaattca agcacaacgt gactacttcg
     2641 gtgctcatac gtacgaaaga aaagataaag aaggcgtatt ccatacacaa tggattgaag
     2701 aataatattg attaaaggat agttgaaaga tactatcctt ttttatatct tttgataaat
     2761 ataaaagaga gtgtgataga gtagctacaa cctataaaaa attacacaag tattatcctt
     2821 gaccttaaaa gcatcccgcc atatttttaa gcattttgtg aagtaataaa atatttactg
     2881 tgttatgata gcatgtacta tagagaaata gaggtactat tttatggaaa agttaaatca
     2941 agttatgtta tatgttgatg atgtcgaaaa agcgaaagca ttttggacag aaactttaga
     3001 atttgttgtt gtaagtgaaa caccgttagc tgaggactat gttgcagttg aagtatcacc
     3061 taccaaagat gctgaaacgt cattgacgat tatggcaaaa gaatttattg aaaagcatag
     3121 cccggaagtg aacctaggaa caccatcttt aatgtttaaa gagaaaaact ttgatgcatt
     3181 atactcaaaa ttaaatgatt taggtttaac tgggcatgat atagtagaaa tgaatggtca
     3241 acgtgtattt aattttcaag atggccaagg taactatttt gcagtta
//
LOCUS       DAMQYT010000224         3271 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMQYT010000224 DAMQYT010000000
VERSION     DAMQYT010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.229"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..1285
                     /gene="mgtE"
                     /locus_tag="PGA33_002459"
     CDS             <1..1285
                     /gene="mgtE"
                     /locus_tag="PGA33_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDG6474241.1"
                     /translation="DEFLALHTYEQSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDI
                     DDDEYELLFDKMNATYASHILEEMSYDNAVDILNELTKPKVASLLTLMNKDDANEIKA
                     LLHYDEDTAGGIMTTEYLSLKAHTPVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGV
                     LSLRDLIVAENDAYIEDIMNERVISVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLG
                     IITIDDILDVMDEEASEDYSRLAGVSDIDSTNDSIIKTALKRLPWLIILTFLGMITAT
                     ILGRFEKTLENVALLAAFIPIISGMSGNSGTQSLAVSVRNITTGEINEQSKFRIALRE
                     AGSGVLSGVVCSTILFTIIVAIYHQPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLN
                     IDPAVASGPFITTINDIISMLIYFGLATSFMAYLI"
     gene            1295..3139
                     /locus_tag="PGA33_002460"
     CDS             1295..3139
                     /locus_tag="PGA33_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HDG6474242.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
BASE COUNT         1086 a          430 c          623 g         1132 t
ORIGIN      
        1 agatgaattt ctagcattac acacatatga acaaagtgag tattttgaag atactaccga
       61 tgaaaataga caaaagattt ttcaatattt atcacctgaa gaagttgcaa atttctttga
      121 tcaattagat attgatgacg atgaatatga gttgctattt gataagatga atgcgacata
      181 cgcaagtcac atattagaag aaatgtcata cgacaatgca gtagatattt taaatgagtt
      241 gactaaacca aaagttgcta gtcttttaac attgatgaat aaagatgacg cgaatgaaat
      301 caaagcatta cttcactatg atgaggatac ggccggcggt attatgacga cggagtattt
      361 atcacttaaa gcgcatacgc ctgttaaaga agcattatta ttggtcaaag cgcaagcacc
      421 agacgcagaa acaatatatg ttatatttgt cgttgatgat gatggtaaat tagtaggtgt
      481 tttatcgcta agagatttaa ttgtagctga aaatgatgct tatattgaag atattatgaa
      541 tgaacgtgtc attagtgtga atgtagcaga cgaccaagaa gatgttgctc aagttatgag
      601 agactatgat ttcatggctg tacctgttat agattaccaa gaacatttgc ttggtatcat
      661 cacgattgat gatattttag acgttatgga tgaagaggct agtgaagact actctcgttt
      721 agccggggta tcagatatcg attcgactaa tgattcaatc attaaaacag cattaaaacg
      781 tttaccatgg ttgattattt taacattttt aggaatgatt actgcgacaa ttttagggag
      841 attcgaaaaa acattagaaa atgtagcgct actcgcagcg tttattccta ttattagtgg
      901 tatgtcagga aattcaggta cacaatcttt agccgtttca gttcgtaaca ttacgacagg
      961 ggaaattaat gagcaaagta aatttagaat tgcattaaga gaagcaggaa gtggtgtatt
     1021 atcgggtgtt gtatgttcaa caatattatt tacaattatt gttgcaatat atcatcagcc
     1081 acttttagca ttaatcgttg caggaagttt aacttgtgcg atgacggtgg ggacgtttgt
     1141 aggttcgatg attccattat tgatgaataa attaaatatc gatccagcag tggctagtgg
     1201 accatttatt acaacaatta atgatattat tagtatgttg atttattttg gtttagctac
     1261 atcatttatg gcttacttaa tttaaggagg agttatggag tttttatctt tagttattgt
     1321 tgttttagca gcgtttttaa ctccaataat tgtcaatcga ttaaatatta atttcttgcc
     1381 agttgttgtt gcagaaattt tgatggggat tgtgattgga aattcatttc taaatatagt
     1441 agaaagggat tcaattctaa atattttatc aacgttaggc tttatctttt taatgttttt
     1501 aagtggttta gaaattgatt ttaaagcttt taaaaaagat aaacgcgcac gtcaaggaca
     1561 aaatgatgat gaatcctcaa ttccagggca tcttaatcta gcgttaactg tatttgcatt
     1621 tattatgatt atttcgattc ttttagcgta tgtatttaaa tggcttggat tagtggatga
     1681 tgtgttatta atggtcatta tcatttcaac tatttcctta ggcgtagttg ttccaacttt
     1741 aaaagaaatg aatattatga gaacaactat agggcaattt atcctattag tagcagtact
     1801 tgcggactta gtaactatga ttttattaac ggtctatggc gcaatcaatg gtcaaggcgg
     1861 cagtacaata tggttaatag gtatattagt tgttttcaca gcaatttcat atattttagg
     1921 tgttcaattt aaaagaatgt catttttaca aaaattgatg gatggtacga cgcaaatcgg
     1981 tattcgtgcg gtatttgcat taataatatt attagtagcc ctagcagagg gagttggcgc
     2041 agaaaatata ttaggtgcat tcttagcagg tgtcgttgtt tcattattaa atccagatga
     2101 agaaatggtt gaaaagttag actcatttgg ttatgggttc tttattccta ttttctttat
     2161 aatggttggt gtagatttaa acataccttc attaattaaa gaaccgaaat tactaattat
     2221 cataccgatt ttaatcgttg catttatcat ttcaaaatta attccagtca tgtttattcg
     2281 acgttggttt gatatgaaaa caacgattgc atcagcattt ttattaacat caacattatc
     2341 gctcgtgata gctgcagcca aaatttcaga aagattaaat gctatttcag ctgaaacgtc
     2401 aggtatatta attttaagcg cagtcattac atgtgtattc gttccgatta ttttcaaaaa
     2461 actgtttcca gttccagatg agtttaaccg taaaattgaa gttagtttaa ttggtaaaaa
     2521 tcaattaacg attcctatag cgcaaaattt aacatctcag ttatatgacg tgacattata
     2581 ttatcgcaaa gacttgagtg atcgtcgtca attgtcagat gatatcacga tgatagaaat
     2641 tgctgattat gaacaagatg ttttagaacg actaggtctg tttgaccgag acatagttgt
     2701 ttgtgctacg aatgacgatg atattaaccg aaaagttgct aaattagcca aagcacatca
     2761 agttgagcgt gtcatttgca gacttgaaag cacaacggac gatacagagt tagttgattc
     2821 aggtattgaa attttcagta gctacttaag taataaaatc ttattaaaag gtttaattga
     2881 aacacctaac atgttgaatt tattaagtaa tgttgaaacg tcactatatg aaattcaaat
     2941 gttaaattat aaatatgaaa atattcaatt acgtaatttc ccattcggag gagacatcat
     3001 cttcgtgcgt attatccgta ataatgagtc gattgttccg catggagata cacaattgcg
     3061 atatggagat cgcttaattg ttaccggtgc taaagaatac gttgatgaat tgaagcaaga
     3121 gttagaattt tatttttaac aataatgata ttaaaatgca gacgtatttt agtacgacgt
     3181 aaaattaatg attttaaaat gctagtatgt atatgatttt gataaataaa tgctttttac
     3241 gtaaatcaag tttgatacag aaaggactaa a
//
LOCUS       DAMQYT010000225         3271 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMQYT010000225 DAMQYT010000000
VERSION     DAMQYT010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.230"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..793
                     /locus_tag="PGA33_002461"
     CDS             <1..793
                     /locus_tag="PGA33_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474243.1"
                     /translation="QRYSNSKIDTEVYARKQLIKKNKALSAENAELRSQMLSSNNDVG
                     HHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLATKHPFFEYARSFDFIIVSDIGL
                     INVDVKSWGEKTFYHFDVPDEHDTEISNSNIEKVVGHYISQQYHDQFNSSRKSIYTFT
                     ETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFNFKVQSTGVIYFSDGTVNIIQG
                     SEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQVDQITAIFK"
     gene            complement(936..1469)
                     /locus_tag="PGA33_002462"
     CDS             complement(936..1469)
                     /locus_tag="PGA33_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDG6474244.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            1608..1796
                     /locus_tag="PGA33_002463"
     CDS             1608..1796
                     /locus_tag="PGA33_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HDG6474245.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            complement(1909..2511)
                     /locus_tag="PGA33_002464"
     CDS             complement(1909..2511)
                     /locus_tag="PGA33_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDG6474246.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVFLDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGWI"
     gene            complement(2508..>3271)
                     /locus_tag="PGA33_002465"
     CDS             complement(2508..>3271)
                     /locus_tag="PGA33_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDG6474247.1"
                     /translation="LFILTMIICTIITYLLYNNYFVAMMVYYVVISLIMLDNFKKMKN
                     REYQKEIAEKNRHINTLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDADVEK
                     VKAELLAINKLSRESLNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQ
                     VLSPTKQSMLVMITREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCE
                     VKSISQRVQHLNGTLAVDSTNGTKIIIEISTGGIA"
BASE COUNT         1084 a          546 c          406 g         1235 t
ORIGIN      
        1 tcaaaggtat agtaatagta aaatagatac cgaagtttat gcaagaaagc aacttattaa
       61 aaagaacaaa gcattaagtg cagaaaatgc tgaattaaga agtcaaatgc tgagttctaa
      121 taatgatgtt ggccaccatg catacaaaaa tgctaaaaga gaattacgca agattttaga
      181 tagctatctc gaaaatggta aattaaaata ttacgatatt atcgttacaa gcaatttagc
      241 tactaaacat ccgtttttcg agtatgcacg ttcatttgat tttatcattg tttcagatat
      301 cggtttaata aacgttgatg taaaaagctg gggtgaaaaa acattttatc actttgatgt
      361 acctgatgag catgatacag aaataagcaa tagtaatatt gaaaaagttg tcggtcatta
      421 cattagccag caatatcatg atcagtttaa ttcatcaaga aagtctatct atacttttac
      481 agaaacagtt caaccaaatc gtgttattta tgatttttat gattatgatc cgtatcaatt
      541 agcagcaaat aatgcgaaag cattaaagga tcatattgaa caaaacttta actttaaagt
      601 ccaaagtacc ggtgttattt actttagtga tggtacagtt aatatcattc aaggttctga
      661 agaaagagat aaatatgttg atacagtttc aacaaaatct tcgttacgac gtattattag
      721 cgaagccata gagctatcta aacacccttt aaataaagaa caggttgatc aaatcacagc
      781 tatattcaag taaaatcaaa ggcataataa tccattacgc tcgatacgca ctatggttat
      841 tatgccttta aattatttct atatataaat ttatggtaat aataaatcga tgcttcaagt
      901 atattttact ttaatatata taatcaccta tttaattatt tactccaaat atttaatttc
      961 tgttgtttag ctttattttg tgcttctata aatacatttc tatatttgcc atttggtgaa
     1021 aaatacttct ctctagcaag tcccttttcc actaattcct tattgtacat acgatcttta
     1081 cttatccata catacgccaa tgttctacca tagcgatctt gtttttcttt atcatattct
     1141 aaataaacat cttgatttgt taatgtcttc ttactataat ttgatgcttc tttgccaaat
     1201 ggttgtacag gcgtattcgg tttcaccgtt tctggcgtat caacccctat aagcctaact
     1261 ttaatttctt taccattttg atttgcaata aatgtatcac catccacaac tctttgaaca
     1321 tgtactttat ctttaccatt taaatcttgt acagtttcat gattcgtccc ctttttgaaa
     1381 ggacccgtat gattcataaa ttgaaatgct aatacaccta caataatgat ggctaccaca
     1441 atcatagcaa gcgatttatt tgacttcata ttgccaccta cttctctttt ttcattccga
     1501 ttaatcatat aaaagttaag aaatatcgtc aatagccata tctacatatt tcaatatttt
     1561 ggaaagctat agcatgatat aatgttattg ttaaggagtg aaaataaatg aacatttcaa
     1621 atgaaaatac agtcacatta attgcagtaa ttatcgcaat tatcattggt attttcctac
     1681 aaatattttt caaattacca ttaatagtga cggcagtact atctatttta ttgggtatct
     1741 ttgtaggctt tatcgtttac ttgattgttt cgttttataa taagcgaaaa aattagttaa
     1801 caaatctaaa ttgcgagtac caaaataaaa gtattgagta agattttgat tattgactca
     1861 ctttccaagt ctcttaaatc aaaacactca atactttttt gtttgtattt agatccagcc
     1921 tttttcattt gcctttttcc aagcatcaaa acgattatct gcaaataatt tatcaattat
     1981 aacagatgta taatttctaa ctgttccatc tgtcaaaaat aatttttcac ttatttcttt
     2041 actacttaaa ccattgccaa tttcccttaa tacaatttgt tctttgggcg ttaatgggtt
     2101 tttatctaca aaaaatgaag tcatcaatgt ggcgctatat tctttctctc cgttatttac
     2161 tttattaatg gtttccacca attcttctat agaacgttct tttaaaacat atgcatccac
     2221 atcattcaca actgcttttt caaagtatcc cggtctttta aaagttgtta caataatcac
     2281 tttaatattc aaatgctttt ttctaatttc cgctaaaact tcaagtccag tcatgcctgg
     2341 catttctata tctaaaaaaa caacgttagg attatattct tcaataagtt tcattgcatc
     2401 gagaccatta tcagtatctg ctaaaatttc aaaatcacca tgtagtttaa ttaattgaac
     2461 cattgcctgt cgtaacatat tttgatcttc tgcaataatt aaagatgtca tgctattcct
     2521 cctgttgaga tttcaatgat tattttagtt ccatttgttg agtcgactgc taaagttcca
     2581 tttaaatgtt gtacacgctg tgaaatactt ttcacctcac aatcactatc gatacctttg
     2641 ccatcatctt caatcataag cagtaattta tgattgttta cagtttttaa tttaccatga
     2701 acttttgaag catttgcatg tttaataaca ttatttatcg cttcacgcgt aatcataact
     2761 aacatagatt gtttagtagg acttaatact tgcgctaatt ctttattttc aaatgtaaaa
     2821 tcaatatcag catcttttaa aactttacgt atactatcaa tctcttcaat aaatgatggt
     2881 aattttacat catcaataat ttctcgaact ttgttcaatg attcacgaga taatttatta
     2941 attgctaata attcagcttt tactttttct acatcagcat ctattagttt ataagctaat
     3001 tctgatttta atgataaact tgcaaacaca tgccctaacg tatcatgtaa gtcttgacca
     3061 attctatgtc gctcttgttc agcaattaat gtattaatat gtctattttt ttctgctatt
     3121 tctttttgat attcacggtt tttcattttt ttaaaattat ctagcattat taacgatata
     3181 acgacataat aaaccatcat tgcaacaaaa tagttgttat acaataagta cgttattatt
     3241 gtacatataa tcattgttag tatgaaaaga t
//
LOCUS       DAMQYT010000226         3233 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMQYT010000226 DAMQYT010000000
VERSION     DAMQYT010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.231"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            168..2318
                     /locus_tag="PGA33_002466"
     CDS             168..2318
                     /locus_tag="PGA33_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDG6474248.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLQEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE"
     gene            2311..2766
                     /locus_tag="PGA33_002467"
     CDS             2311..2766
                     /locus_tag="PGA33_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDG6474249.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
BASE COUNT         1240 a          414 c          613 g          966 t
ORIGIN      
        1 gtgtcatttt caataatctt agtgcgtttt aaaatatagt atgacctaat cattcgtttt
       61 aaatgttttg gaagtgaaaa ttacattaag tatcatacct taatagaagt attttagaat
      121 atgttaaaat aaatgagtaa atttaagaaa aagtgtgggt taagtaaatg gacaatcaat
      181 tgattaattc aatcatagag aaatatcaat ttagtaaaaa acaaattgaa gcagtattaa
      241 cactgctaga agaaaaaaat acagtaccat ttattgcgag gtatcgaaaa gagcaaactg
      301 gtggactaga tgaagttcaa ataaagcaaa ttgatgacga ataccaatat atggtcaatt
      361 tacaaaaacg taaagaagaa gttatcaaaa atatagaaca gcaaggatta cttactgagg
      421 aattaaagaa ggatatttta aaacagaaca aattacaacg tgttgaagac ctatataggc
      481 cttttaaaca aaagaaaaag acaagggcaa ctgaggcgaa acgtaaaggg ttagagccat
      541 tagcgatatg gatgaaggca cgtaaacatg aagtctcaat tgaagaaaaa gcacaacaat
      601 ttataaatga agaagtgcaa tcggttgaag atgctatcaa aggtgcacaa gatattattg
      661 cggaacaaat ttcagataat cctaaatata gaacaaaaat tttaaaagat atgtatcatc
      721 aaggtgtgtt aactacatct aaaaagaaaa atgctgaaga tgaaaaaggt atttttgaaa
      781 tgtactatgc atatagtgag ccaattaaac gcattgctaa tcatagagtt ttagctgtta
      841 atcgtggtga aaaagagaaa gtattatctg taaagtttga attcgatacg acatcagtag
      901 aggatttcat tgcacgtcaa gaaatcaatc ataataatgt aaatcgcagt tatattttag
      961 aggcgattaa agatagcttg aaacgcttaa ttgtcccttc gatagagcgt gaaatccatg
     1021 ctgatttaac tgaaaaagct gaaaatcatg caatagatgt ttttagtgaa aacttaagaa
     1081 atctattact gcaacctcca atgaaaggta aacaaatatt aggcgtagat ccagcattta
     1141 gaacaggttg taaattagca gtcattaacc cattcggtac ttttatagca aaaggtgtga
     1201 tttatccgca tccaccagtt tctaaaaaag aggcagcaga gaaggatttt gtacaaatgg
     1261 ttaaagcgta tgatgtgcaa ttaattgcaa ttggcaatgg tactgcaagt cgtgaaacag
     1321 aacaatttgt tgcagattta attaaaaagc atcagttgcc agtacaattc atcattgtca
     1381 atgaagcggg cgcttcagta tactcagcat cagaaattgc tagagatgaa tttcctgatt
     1441 ttcaagtgga agaaagaagt gccgtatcaa ttggacgacg cgttcaagat ccattaagtg
     1501 aattagttaa aatcgatcct aaatctattg gtgtcggaca ataccagcat gatgttaatc
     1561 aaaaagcact agaaaatgca ttaactttcg ttgttgaaac agcagttaac caagtaggtg
     1621 ttgatgtcaa tacagcatca tcatcattat tacaatatgt gtctggtcta agttcgcaaa
     1681 ttgctaaaaa tataattgca tatagagaag aaaatggcgc aataaagcat aataaagagt
     1741 taagcaagat taagcgtttg ggtgctaaaa cttttgagca gagtatagga tttttaagaa
     1801 tagtagacgg ttcagagcca ttggataata catcgattca tcccgaaagc tataaagtaa
     1861 cgtatcagtt attagataaa ctcggatttg gtggcaatga tttaggtagt gatgcattaa
     1921 aagctaaatt aaattcattg gatatggatg aactggcgat tgaattacaa gtcggtgtac
     1981 ctacattaga ggatattatt aaatccttga aagcacctaa tcgagatcca agggacgagt
     2041 ttgaaacacc aatccttaaa tccgatgtgc tatcaattga agatttacaa gaaggaatga
     2101 aattgagtgg tacagttaga aatgtagtag attttggagc atttgtagat ataggtgtta
     2161 aacaagatgg acttgtccat gtttcgaaac tttctaagaa gttcgtaaaa aatccaatgg
     2221 atattgtcag tgttggtgat atcgttgacg tttggattta cagtattgac aaaaataaag
     2281 acaaagtatc attaacgatg attgatccac atgaataatg acaaacttca gcgaatggtt
     2341 gagaatcttt cggaagaaaa atttggacgt acgtttcgac attgtgcata ttttaataaa
     2401 aggttacgta caacaggcgg acgttatctt ttaaagtccc atgatattga aattaatcca
     2461 aaacaatatg agcattatgg agaagatgct gttgtaaaaa ttatactgca cgaattgtgt
     2521 cattatcact tgcatattgc tggtaaaggt tatcaacata aagatcaaga ttttaaacga
     2581 ttgagccaac aggttggagc gcctagattt tgtaacagca ttgaaagcta tcaacaacgg
     2641 gcaaattatg aatattattg cactaaatgt catgcaaaat atatacgtat ccgtaaagtt
     2701 gatacgaatc gtatgcgttg cggacattgc aatggaaaat taagaatgaa aagacaattg
     2761 aaataagtac aatacaaata gagctaattc gtgtatatgt aagattattt atgagttgta
     2821 tggaatttat atagaattta tcagtgagtt attttgattg gtaaacatag taattgaata
     2881 agaagaaatt gtgtttataa aatgtttaaa gccgagattg tcgtaattta atacttcgca
     2941 atctcggctt ttctacttat gatataggta atggcataaa ttgtggcttg ataggatgaa
     3001 attaaaatga ttagattaaa aatcttggcg tattttagta ataattaata tataaacttt
     3061 acccaattaa agctttaaag tagcacaaaa gataattata catttttcat gacattttga
     3121 ttgaactttt ctattgactg tacagcttat agttagtatg attaaacacg ttccttaaaa
     3181 aagaacaaat taaataaact ttgatttgaa agacagtcaa ttctaaattt aat
//
LOCUS       DAMQYT010000227         3218 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMQYT010000227 DAMQYT010000000
VERSION     DAMQYT010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.232"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            1..1152
                     /locus_tag="PGA33_002468"
                     /pseudo
     CDS             1..1152
                     /locus_tag="PGA33_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669709.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="MAP domain-containing protein"
     gene            complement(1476..2762)
                     /locus_tag="PGA33_002469"
     CDS             complement(1476..2762)
                     /locus_tag="PGA33_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDG6474250.1"
                     /translation="MNPLAQSLNEQLQQSNATAFTMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            2962..3060
                     /locus_tag="PGA33_002470"
     CDS             2962..3060
                     /locus_tag="PGA33_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474251.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
BASE COUNT         1174 a          449 c          534 g         1061 t
ORIGIN      
        1 ctagtcaaat aaaatcagta ctgaagcatg atagaggtat tagtgaacaa gatttaaaat
       61 atgctaagaa agcttattat actgtttatt ttaaaaatgg tggtaaaaga atcttacagt
      121 tgaattcaaa aaattacaca gcaaacttag ttcatgcgaa agatgttaag agaattgaaa
      181 ttactgttaa aacaggaact aaagcgaaag cagacagata tgtaccatac acaattgcag
      241 taaatggcac atcaacacca attttatcag atttaaaatt tacaggtgac ccacgtgtag
      301 gctacaaaga tatctctaaa aaagttaaat cagtattgaa gcatgataga ggtatcgggg
      361 aacgtgaatt aaaatatgca aaaaaagcaa cttacacagt acattttaaa aatggaacga
      421 aaaaagtgat taacataaat tcaaatatta gccaactgaa tctgctttat gttcaagata
      481 ttaaaaagat agatattgat gttaaaacag gaactaaagc gaaagcggat agctatgtac
      541 catatacaat tgcagtaaat ggcacatcaa caccaatttt atcaaaactt aaaatttcga
      601 ataaacaatt aattagttac aaatatttaa atgacaaagt gaaatctgta ttaaaaagtg
      661 aaagaggcat cagtgatctt gacttaaaat ttgcgaaaca agcaaaatat acagtatatt
      721 tcaaaaatgg aaagaaacaa gtagtgaatt taaaatcaga catctttaca cctaatttat
      781 ttagtgccaa agatattaaa aagattgata ttgatgtaaa acaatacact aaatcaaaaa
      841 aaaataaata aatctaataa tgtgaaattc ccagtaacaa taaataaatt tgaaaacata
      901 gtttcaaatg aatttgtgtt ctataatgca agcaaaatta caattaatga tttaagtata
      961 aaacttaaat cagcaatggc aaatgatcaa gggataacta aacatgacat aggacttgct
     1021 gaacgcgcag tgtataaagt gtattttaaa aatggttcgt caaaatatgt agacttaaaa
     1081 actgagtata aagatgaaag agtatttaaa gcaactgaca ttaaaaaggt agatattgaa
     1141 cttaaattct aatctttaat tttataataa ggcatctcac aatcgtgggg tgccttttac
     1201 atttgtagag atgtgatgct tgaatgtttg aagtgatttg ccaaaagtta tataaattgg
     1261 ttctataata aatcggactg atagaaaaag ttttttactt ttcatctgtc cgattttttg
     1321 attttgttag gagagatgta taaaactaat taggtatagt gtttgacaag ttgctgaata
     1381 aaatatatct tatgatactt tgaaagaagg aataatttta aaaataaaaa accaataatc
     1441 cgagtcatac tcatcagatt attggttgaa attaattatc ttaagtcatc aatcgcttta
     1501 gcaattgaat caaatacatg tgggatatct tctttttcta cacagctaaa cgcaatacga
     1561 atatctgttg cattaagtgc aataacacca attgaatatt tttcaattaa atgtttacga
     1621 agttcttcag ggtcaacatc atgaactttt attgccataa agtatccaga gttaaagtca
     1681 taggcttgcc aatgtgaatg atattgatca gcatagacaa cttccttagt gacttcatag
     1741 cgttctttta atgtttgaat attttgttcg atttctttat cgaattgttt attatttttt
     1801 aatacatgct taacagcgct ttgtgttgga agaggtccac tagaaatgtt acttcgtata
     1861 agacctttta ctttggcttc taatacttct ttagttgttt gatcagacgt tccaaatgtc
     1921 ataaacccaa cacggaatcc ccatgcgaaa aattcttttg ttgcaccatc taaacgaaca
     1981 ggtaatattg catttgaatt taaattagat aatgcagtaa ataatgattg agtatacaca
     2041 tcttcataga ataaaccata gtatgcgtca tccacaacag ctataacttt tgtaccttta
     2101 tcagctaatg ctttaattgc atcgacgatg gtagtcactt ctttatgcgt aggtgtgtaa
     2161 cctgtcggat tattaggata atttaaaatc ataatgactt tatctttatt gtatgattgt
     2221 aaagcttcta caagtgaatc agtagtataa tgcccgtctt tatcaaagat aggatatgtt
     2281 tgaaggtttg caccatttct cgtattgaaa acaagtttgt aattacccca attatgctct
     2341 ggtaacaaga tagtgtcacc ttgatttaca aataaatcgc caactaaaga taaaccatgt
     2401 gttaatgcat tcgtaacaat tggtagtgac atgttgtcga ttgatagctc tggattgtca
     2461 cgcaacattt tttgttgcca taaatcacgt aattcttcga tgccttgtgg tggcgcataa
     2521 gggaatattt catctggagt taaatcatta aacattgcat ctaaagatga tgcaaacatt
     2581 tttccgtctt tgtttgtcgc catacctata gttgcattat atgttgtact ctttgcttca
     2641 gcagattgag ataaaatgcc ttttggataa aacatatttt gacctaagtc agataacatt
     2701 gtaaaggcag ttgcatttga ctgttgaagt tgttcattta agctttgggc taaaggattc
     2761 attattgctt aacctctctt taaaaaaatt gtatgcctat atattatctt caattatacc
     2821 aaagataaag tgttgttggt gaattaacga ataatatgta aaatctgctg ccaaaatacg
     2881 aacatatgtt ctgtttttat gttagaatag gtgtaggtca tccttcagta tgatacttta
     2941 gtcgaacgga ggtgtagtgt aatgatgtgg tggcaagaag ggatgatgac acttattaca
     3001 ggtggcttac taattatttt tcgactttgg ttagaactga aatggaaaaa taaaaaatga
     3061 tcctacacac ataaataacc ccaagtctgc tggtaccaga cttggggttg tgtagtataa
     3121 ttctgtggat acacttaaat aatatcattg aacacaatat atcgcaatca ataatcattt
     3181 tgattgaatg gttttgttgc gctattttag ttgaaagt
//
LOCUS       DAMQYT010000228         3203 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMQYT010000228 DAMQYT010000000
VERSION     DAMQYT010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.234"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            173..2215
                     /gene="lip1"
                     /locus_tag="PGA33_002471"
     CDS             173..2215
                     /gene="lip1"
                     /locus_tag="PGA33_002471"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HDG6474252.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKKSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDSNANNNLPSQSRIKEAPSLNKLDQTSQREIVNETEIEKVQPQQNNQANDKITNY
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNHDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTKATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKLTDTKQA"
     gene            complement(2662..>3203)
                     /gene="icaC"
                     /locus_tag="PGA33_002472"
     CDS             complement(2662..>3203)
                     /gene="icaC"
                     /locus_tag="PGA33_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HDG6474253.1"
                     /translation="FHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERY
                     LVIMIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNT
                     IQMISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREF
                     YIFRFIIGKQPYKLNINAY"
BASE COUNT         1289 a          486 c          581 g          847 t
ORIGIN      
        1 ttctctttgg agaatatatt ttttaaatac aaatgtaaac gctttctcgt caaattaaac
       61 aatagaaagg atggtcatta tgagtgcttg gttaagtaaa ttatttgagt ttattcctcg
      121 aataattatc aatttgttta tctaaaataa aaaaatagag gtgctgacaa tgatgaaaag
      181 tcaaaataag tatagtattc gtaaatttag tgtaggtgca tcttccattt taatagctac
      241 attactattt ttaagtggtg gacaagcaca agcagctgag aagcaagtga atatgggaaa
      301 ttcacaggag gatacagtta cagcacaatc tattggggat caacaaacta gggaaaatgc
      361 taattatcaa cgtgaaaacg gtgttgacga acagcaacat actgaaaatt taactaagaa
      421 cttgcataat gataaaacaa tatcagaaga aaatcatcgt aaaacagatg atttgaataa
      481 agatcaacta aaggatgata aaaaatcatc gcttaataat aaaaatattc aacgtgatac
      541 aacaaaaaat aacaatgcta atcctagcga tgtaaatcaa gggttagaac aggctattaa
      601 tgatggtaaa caaagtaaag tggcgtcaca gcaacagtca aaagaggcag ataatagtca
      661 agattcaaac gctaataaca atctaccttc acaaagtcga ataaaggaag caccatcatt
      721 aaataagtta gatcaaacaa gtcaacgaga aattgttaat gagacagaaa tagagaaagt
      781 acaaccacaa caaaataatc aagcgaatga taaaattact aactacaatt ttaacaatga
      841 acaagaagtg aaacctcaaa aagacgaaaa aacactatca gtttcagatt taaaaaacaa
      901 tcaaaaatca ccagtagaac caacaaagga caatgacaag aaaaatggat taaatttatt
      961 aaaaagtagt gcagtagcaa cgttaccaaa caaagggaca aaggaactta ctgcaaaagc
     1021 gaaagatgat caaacgaata aagttgccaa acaagggcag tataaaaatc aggatcctat
     1081 cgttttagtg catggtttca atgggtttac agatgatatt aatccttcag tgttagctca
     1141 ttattggggc ggtaataaaa tgaacattcg ccaagattta gaagaaaatg gttacaaagc
     1201 ttatgaagca agtataagtg cttttggaag taactatgac cgcgcagttg aactttatta
     1261 ttatatcaaa ggcggtcgtg tagattatgg tgcagcacat gcagcaaaat atggacatga
     1321 acgttatgga aaaacatacg aaggaattta caaagactgg aaaccaggac agaaggtaca
     1381 cctagttgga catagtatgg gcggtcaaac gatacgtcaa ctagaagaat tactgcgtaa
     1441 tggtaatcgt gaagaaatag agtatcaaaa gaaacatggt ggcgaaattt ctccactatt
     1501 caaaggtaat catgacaata tgatttcatc aattactact ttaggaacac cacataatgg
     1561 tacacacgca tcagatttag ctggtaatga agctttagtg agacaaatcg tatttgatat
     1621 cggtaaaatg tttggtaata aaaattcaag agtagacttc gggttggctc aatggggtct
     1681 aaaacagaag ccaaatgaat catatattga ttatgtcaaa cgcgttaaac aatctaattt
     1741 atggaaatca aaagataatg gattttacga tctgacgcgt gagggtgcaa cagatttaaa
     1801 tcgtaaaacg tcgttgaacc ctaacattgt gtataaaaca tacactggtg aagcaacgca
     1861 caaagcatta aatagcgata gacaaaaagc agacttaaat atgtttttcc catttgtgat
     1921 tactggtaac ttaatcggta aagctactga aaaagaatgg cgagaaaacg atggtttagt
     1981 atccgttatt tcttctcaac atccatttaa tcaagcttat acaaaagcga cagataaaat
     2041 tcaaaaaggc atttggcaag tgacgcctac aaaacatgat tgggatcatg ttgactttgt
     2101 aggacaagac agttctgata cagtgcgcac aagagaagaa ttacaagatt tttggcatca
     2161 tttagcagac gatttagtga aaactgaaaa gctgactgat actaagcaag cataatttat
     2221 aaagtaaagg gaggaattaa taatgactgc agacttcttt caattaatcg gatcattatt
     2281 tagaattcta aaagaattat tcaagtaaaa cattggcgag gccccaacat aaagaatttc
     2341 gaaaagaaat tctacaaaca atgcaagttg gcggggcccc aacaaagaag ctggcggaaa
     2401 gtcagcttac aataatgtgc aagttggcgg ggccccaaca tagaagctgg cggaaagtca
     2461 ggttacaata atgtgcaagt tggggtggga cgacgaaata aattttgcga aaatatcatt
     2521 tctgtcccac tcccattggc atttacgaag tttaaatgtg caattagaat atatgtataa
     2581 caatattaaa cacgcggtaa aacgaagtca gtcaattcaa actgatttcg cccaccgcgt
     2641 gtttttaaca tagcttaata attaataagc attaatgttc aatttatatg gttgttttcc
     2701 aataataaac ctaaagatat agaattcacg caatatcatg ccgacaccta tacataatcc
     2761 taaaatgaat agtagtgata tcgctagaaa gaccattgta ttatcctcaa atatatttgt
     2821 atatgcaaac aatgagtcta gaatgattgg atgtaataaa taaataaaga atgagaaagc
     2881 actaatcatt tgaatcgtat taaataacat tgttttaaaa tgcgtgcaaa tacccaagat
     2941 aacaataaac ataatactat tatatggtgt taatgaatat gaaaagctgg taacgttcca
     3001 atagtctcca tttgctaacg caataaacac aaaataagta gctacagcta atacaatcat
     3061 aataactaaa taacgttcta agaaatttaa tacacgttcg tagttataac ccatatatgc
     3121 acctaagaag aaataaaaaa tccatccgaa tattatagta ttttcactta atggataata
     3181 gtgtagcacg gtatcgtgaa acg
//
LOCUS       DAMQYT010000229         3184 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMQYT010000229 DAMQYT010000000
VERSION     DAMQYT010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.235"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(15..90)
                     /locus_tag="PGA33_002473"
     tRNA            complement(15..90)
                     /locus_tag="PGA33_002473"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55..57),aa:Ala,seq:tgc)
     gene            complement(114..187)
                     /locus_tag="PGA33_002474"
     tRNA            complement(114..187)
                     /locus_tag="PGA33_002474"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(151..153),aa:Pro,seq:tgg)
     gene            complement(208..284)
                     /locus_tag="PGA33_002475"
     tRNA            complement(208..284)
                     /locus_tag="PGA33_002475"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(248..250),aa:Arg,seq:acg)
     gene            complement(290..378)
                     /locus_tag="PGA33_002476"
     tRNA            complement(290..378)
                     /locus_tag="PGA33_002476"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(342..344),aa:Leu,seq:taa)
     gene            complement(386..460)
                     /locus_tag="PGA33_002477"
     tRNA            complement(386..460)
                     /locus_tag="PGA33_002477"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(426..428),aa:Gly,seq:gcc)
     gene            complement(494..566)
                     /locus_tag="PGA33_002478"
     tRNA            complement(494..566)
                     /locus_tag="PGA33_002478"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(531..533),aa:Lys,seq:ttt)
     gene            complement(573..648)
                     /locus_tag="PGA33_002479"
     tRNA            complement(573..648)
                     /locus_tag="PGA33_002479"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(613..615),aa:Thr,seq:tgt)
     gene            complement(665..740)
                     /locus_tag="PGA33_002480"
     tRNA            complement(665..740)
                     /locus_tag="PGA33_002480"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(705..707),aa:Val,seq:tac)
     gene            complement(753..867)
                     /gene="rrf"
                     /locus_tag="PGA33_002481"
     rRNA            complement(753..867)
                     /gene="rrf"
                     /locus_tag="PGA33_002481"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1425..2912)
                     /gene="lysS"
                     /locus_tag="PGA33_002482"
     CDS             complement(1425..2912)
                     /gene="lysS"
                     /locus_tag="PGA33_002482"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDG6474254.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            complement(2965..3057)
                     /locus_tag="PGA33_002483"
     CDS             complement(2965..3057)
                     /locus_tag="PGA33_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474255.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
BASE COUNT          957 a          697 c          483 g         1047 t
ORIGIN      
        1 gtgtaaaaaa taaatggtgg agactagcgg gatcgaaccg ctgacctcct gcgtgcaaag
       61 caggcgctct cccagctgag ctaagccccc ataaaatgga atttaagaag taatcgggaa
      121 gacaggattc gaacctgcga ccccttggtc ccaaaccaag tgctctacca agctgagcta
      181 cttcccgtta ttcaattaat ttaaaaatgg cgcgcccgat aggagtcgaa cccataacct
      241 cttgatccgt agtcaaacgc tctatccaat tgagctacgg gcgctaaaat ggtgccgagg
      301 accggaatcg aaccggtacg gtgatctctc accgcaggat tttaagtcct gtgcgtctgc
      361 cagttccgcc accccggcaa aataatggag cagaagacgg gattcgaacc cgcgacccca
      421 accttggcaa ggttgtattc taccgctgaa ctacttctgc attattttgc taaatataaa
      481 aattaaatcg taatgagcca tagaggattc gaacctctga ccctctgatt aaaagtcaga
      541 tgctctacca gctgagctaa tggctcaaaa gatggtgccg gccagaggac ttgaaccccc
      601 aacctactga ttacaagtca gttgctctac caattgagct aggccggcta tatctaagaa
      661 taaatggtgg agaatgacgg gttcgaaccg ccgaccctct gcttgtaagg cagatgctct
      721 cccagctgag ctaattctcc gatttgtcat ttgcctggca acgttctact ctggcggaac
      781 gtaagttcga ctaccatcga cgctaaggag cttaacttct gtgttcggca tgggaacagg
      841 tgtgacctcc ttgctatagt caccagacaa aataacttac ttattcatga tacacgctta
      901 attaataagg tgcaatagtt aattttacac tttgatgtaa actttgtcac atcagctttt
      961 agtgtgtttc gtctgttttt ggcgactttt atatattact tcattcaatt gataaagtca
     1021 acacttttaa tcaacttttt tattcttcaa aactgacatt tctcgtgccc tctaattcat
     1081 aacactttta gtattctatc atacacatat gtacaaaacc atacacattc tatacaattt
     1141 taaagtactt ttatagctga ttaagataaa ttatactaat atagcaattt ttgttataag
     1201 atatctcaat gtctatcgtt aacatttctc atccgaacta ctctatatac gtatcgtcat
     1261 tttcatctat cagccaacta ccttgtttaa cagtcaaaaa tagcaccact atcgttaact
     1321 attactatca aaaacattca aacacgaact aattaccata attaagaaag ccctcgcata
     1381 cacatgttgt atacacgaga gctcttacta acaatcaacg tcatttattt ttgtctcata
     1441 taagggaata ataatacgtc tctgattgat ggagagtcag ttaataacat aaccaatctg
     1501 tcaataccga taccaagacc acctgtcgga ggcataccat attctaacgc ttcaatgtaa
     1561 tcttcatcca tttcatgcgc ttcatcatta ccttgcgctt tttcaacaag ttgcgcttca
     1621 aaacgacctt tttgatcaat aggatcattt aattcagtaa atgcatttgc atgctctcta
     1681 cctacaatga acaattcgaa acgatcagta aatctaggat cttcaggatt tttcttcgct
     1741 aaaggtgaaa tttcagtcgg atgaccatag ataaacgttg gctgaataag tgtttcttca
     1801 actttttgct caaagaattc atttaaaata tgaccatatt tcattgtatc tttaatttca
     1861 ataccatgtt ctttagctaa agctttagct tcttcatcac ttttaacttc ataaaaatct
     1921 acaccagtag cttcttttac agcatcaaca atatgcaaac gagtccaagc agattctaaa
     1981 tcgatcgttt ccccattgta ttgtactttt gcagaaccta acacttcatt ggcaatatgt
     2041 ctcaccatag attctgttaa atccataatg tcatgataat ctgcatatgc ttcatataat
     2101 tcaatcattg tgaattcagg gttatgtcta gttgatacac cttcattacg gaatactcta
     2161 ccaatttcat atactttttc aagtccaccg acaattaaac gttttaaatg caactcaata
     2221 gcaatacgca tgtataacgt tgcatctaat gcattatgat gtgttacaaa tggtctagca
     2281 gctgctccac cagcaatttg gtgcatcata ggtgtttcta cttccaagaa acctttatta
     2341 tttaaataat tacgcatttc ttgaatgatt ttactacgat taataaatgt acgagtgcta
     2401 tcttcgttcg taattaaatc taaatatctt tgacgatatc tctgttcaat atcctgtaaa
     2461 ccgtggaatt tatccggtaa tggtcgcaat gatttagtta gtagcgtgaa tttcttcgct
     2521 ttaaccgata attcgccagt atttgttttg aacattacac cttcaacacc aacgatatcg
     2581 cctaaatcag catttttcca taaatcaaat tcgtcatcgc caacttgatc tttacgaacg
     2641 taaatttgaa tttgtccagc taagtcctga acgtgtgcaa atcctgcttt acctttacca
     2701 cgcttagtca ttaatcgtcc agctatagcg acatgactat ccgcttcttt ttctaccaat
     2761 tcttctttag aatactggtc ccactcttct ttcaaatcac tagataaacc tgaacggtca
     2821 aatttagaac caaacgggtc tataccaaga tcatataatt cttgtaattt ttgacgtcga
     2881 accaacattt ggtcattcat ttcttctgac ataactttct ctcctttaac ttgttgtaat
     2941 cctttagtat tccaactggt acactcaaac attttccttt aaagttttat aaaatatcag
     3001 acatttttgc tcaacataca tctctttcga tgtttctaat actttatcat taaccattac
     3061 accttaaagt tatgatgtgg catgttttct tatattcata acatcaattt tatcactttt
     3121 ttaattttac aagagcttgt aaatgtaata aagcaatttc tgttttcgtt tatgaattac
     3181 ttat
//
LOCUS       DAMQYT010000230         3172 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMQYT010000230 DAMQYT010000000
VERSION     DAMQYT010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.236"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(30..122)
                     /locus_tag="PGA33_002484"
     CDS             complement(30..122)
                     /locus_tag="PGA33_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474256.1"
                     /translation="MFIFKTTSKSHFHNNVKSLECIKIPINKNR"
     gene            411..1547
                     /locus_tag="PGA33_002485"
     CDS             411..1547
                     /locus_tag="PGA33_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HDG6474257.1"
                     /translation="MSVNARDLLVLHTNVNRLVGEEIFANKCLANNDVQIMNSIKKLI
                     EAELLTTTNDFEVSIYKKTRPELQSILKSFGIKTTGNKPDLIKRIDDNFHIINNLDLP
                     YVYIPTKKGEEILKKTEYLTSFIQSYGEISLERAYYLVENYIDENCDDKVAEIYKFEF
                     QRKYDNGEFDFNHGYNFELNMLIDHYKRDVKDYDNARKYSNIYLYFGLRDFLKKLMSN
                     YSYYDSKGNIDLNEIQNDLNRFINSSASGMYERLIYNENLSNNIMFELFKKDTQDYSD
                     LEEQLIEKFINYVVSNVKKESRSNTLIELSKILENGYTIDKEEFKKEDDYLSKYIFTD
                     IDYLKKLESKINVAIDIRSGEIHLVLDDDSLDILIQNQKYGNEF"
     gene            1590..2073
                     /locus_tag="PGA33_002486"
                     /pseudo
     CDS             1590..2073
                     /locus_tag="PGA33_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837446.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2234..2338
                     /locus_tag="PGA33_002487"
     CDS             2234..2338
                     /locus_tag="PGA33_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474258.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            complement(2838..>3172)
                     /locus_tag="PGA33_002488"
     CDS             complement(2838..>3172)
                     /locus_tag="PGA33_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDG6474259.1"
                     /translation="FNTQSLSQSVAENIIRVYTVMTIILFVVIHAIILMGIVQYYLKR
                     GQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVETKTEKAQDSLNDNTTKTMTSD
                     KPEDNQPK"
BASE COUNT         1167 a          366 c          539 g         1100 t
ORIGIN      
        1 ataataagta tttggaactt tattgcattt tatctatttt tattaattgg aatctttata
       61 cattctaatg atttaacatt attatgaaaa tggcttttag atgttgtttt aaaaataaac
      121 actttgaaat ataacctctt gctgatagat taagaaaata tattcttgaa ttttaagggg
      181 ggataattac aatcttagtt cttttaactg gtactattat caatgaatca taaaattcga
      241 aacatacgag tttttagact atgagagaca cattcatttg cattgagaat cattttttta
      301 gtgtataatc tatgtaaatc gaattagaat atagagattt tgtttgttcg gatgtatttt
      361 ttattcatac ttaataaatt tgaaaagcaa aaatgaggag atgaaagcta gtgagcgtaa
      421 atgcacgtga tttattagta ttacacacta atgtaaatag attggttgga gaagagatat
      481 ttgctaataa gtgccttgct aataatgatg ttcaaattat gaattcaata aaaaaattaa
      541 ttgaagctga gttattaaca acgactaatg attttgaagt tagtatatat aaaaagacaa
      601 gacctgaatt acaaagtatt ttaaaaagtt ttggtataaa aacaacaggt aataaacctg
      661 acttaattaa acgtattgac gacaattttc atattattaa taacttagat ttaccatatg
      721 tatatatacc aactaagaaa ggagaagaaa ttttaaagaa aacagagtac ttaacctctt
      781 ttatacaaag ttatggtgaa atttctcttg agcgtgctta ttatttggtt gaaaactata
      841 tagatgaaaa ttgcgatgat aaagttgcag aaatatacaa gtttgaattt caaagaaaat
      901 atgacaatgg cgagtttgat tttaatcatg gatataattt cgaattgaat atgttgatag
      961 atcactataa aagagatgta aaagactacg ataatgccag aaagtattca aatatttatc
     1021 tttactttgg tttgagagat ttcttaaaaa aattaatgag taattattca tattacgata
     1081 gtaaagggaa tatagatttg aacgaaatac aaaacgatct gaatagattt atcaactcta
     1141 gcgcttctgg tatgtacgag cgattaatat ataatgaaaa tttatccaat aatattatgt
     1201 ttgaattatt taaaaaggac acacaagatt atagtgattt ggaagaacaa ttgattgaaa
     1261 agttcataaa ctatgtagtg tctaatgtaa aaaaagaaag taggagtaat actcttatag
     1321 agttatcaaa aattttagag aacggatata caattgataa agaagaattt aaaaaagaag
     1381 atgattatct ttctaagtac atatttactg acatagatta tttgaaaaag ttagaatcaa
     1441 aaataaacgt tgctattgat attcgaagtg gagaaattca tttggtatta gatgatgata
     1501 gccttgatat attaatacaa aatcaaaaat acggcaatga gttttgagtc ataactaaat
     1561 ataatatgtt gaagagaggt tttatttaaa tggctaaaat tggttatgat cgtgtatcaa
     1621 cgaaagatta atatcgatac acttaaagaa tttgggtgtg ggtgtgtata tagtgaaaga
     1681 ataagtggtg tcaaacgtac agagcttgtt aagtgtttag attatttacg agagagcgac
     1741 acattagttg tctatcaact tgatcggtta ggtagaacga caaaactatt aattgaatta
     1801 tcacaatgat tcgatgataa cggaattgac ttacaaatta gtaacatgaa catttcaacg
     1861 aaagacacaa tgggcaaaat gttttttacg atgatgagtg catttttcag gattagaagt
     1921 taatttacta tgtgagtgta taaaatagac ttagcagcaa caagagcgag aggccgaaaa
     1981 agcgggcgcc cctctttacc agagaataaa aaatgagaaa ttaaattttt atatgatgaa
     2041 caaacgataa caggggaaga aatagctagt tagacaaggg tatgtcactc aactgtttat
     2101 cgagttgatt aagaaatgaa aaaacttata ctatgaatta ctgtttaaaa gtgtgcatgt
     2161 tataatattt attgagcaag ttggatagat ggtggctaat ctcttaataa aggggtgatg
     2221 cctatggtta tagttgttac tcctagaaag gactagcatg tctgattttg aaatgcttat
     2281 ggttgtatta acaatcattg gtttagtatt gattagtact caagaccata aaaaataacc
     2341 ttctattcgc tttgaccggc atttttgaag gctattttta aataaaatat aaggtcaccg
     2401 tctttttaac gggctcatta gggtaacatg tttccgagtg ttgccctttt tgtgtttcaa
     2461 gagttaatga ttttcatctt ttgcttctac ttactacaaa tatattttaa cacatttttc
     2521 ttatgaattg tagttctgaa cataatcaga attaataaaa ccaactttcc atacagcaga
     2581 aaatacaatt aaaagtatag aatgtaatca cgaattatat aaaaagtatt ggagacctct
     2641 tcatatatag ataattcact tagttatttt agaaagaagc ccctaacaac taaagttgaa
     2701 aaatagagga acacagttgg atgctacgca tcaactgcat aagagcccct aacaactaaa
     2761 gtttaaaagt aggagcgcct gcgctaagcg catgcataag agcccctaac aactaaagtt
     2821 gtaaggggct ctaaaattta ttttggttga ttgtcttctg gtttatctga agtcattgtt
     2881 tttgttgtat tatcatttaa tgaatcttgt gctttttctg tttttgtttc aacagatgtt
     2941 gtcactttat cattttctac ttttgtattt tttggttcag tgacaacttt tttattcttg
     3001 tcagctgttt ttactttatc tttagtaatt ttttgaccac gttttaagta atattgaacg
     3061 atacccatta aaatgattgc atgaataaca acgaataaaa taattgtcat tactgtataa
     3121 actcttatga tattttctgc aacactttga gagagtgatt gcgtattaaa tg
//
LOCUS       DAMQYT010000231         3167 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMQYT010000231 DAMQYT010000000
VERSION     DAMQYT010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.237"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..415)
                     /locus_tag="PGA33_002489"
     CDS             complement(<1..415)
                     /locus_tag="PGA33_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDG6474260.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVT"
     gene            776..1573
                     /locus_tag="PGA33_002490"
     CDS             776..1573
                     /locus_tag="PGA33_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDG6474261.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
     gene            complement(2161..>3167)
                     /locus_tag="PGA33_002491"
     CDS             complement(2161..>3167)
                     /locus_tag="PGA33_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDG6474262.1"
                     /translation="SYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKTA
                     ILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYGI
                     PSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFKV
                     IFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVEP
                     QLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLHF
                     PLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLFF
                     "
BASE COUNT         1140 a          556 c          468 g         1003 t
ORIGIN      
        1 atgtaacgaa taaatatttg cctatactcg atgcatctcc aacgaagaaa taaccaataa
       61 taccaaatgc tataaatgca aaaattgcta atgcgatatt tttatagaaa atatctggtc
      121 ctaaattttg taaatacgtg gtaaacgttg cgtatgacaa taagccaatc actacagcgt
      181 aaatatgtga aataattaaa ccgaaccgtc tcgctctttc aaaaacaagt gttgctaaaa
      241 taataatcag taatccaaat gacaagggtt gtcgccaact taatggtaaa aactgaccta
      301 gatagcatcc tatgccaaat ataatccaat aatacataaa ataaagccac actttactta
      361 ttgattgagc atgctggttt tgatgataat atgaatgatt tgtattttgc gacaaaataa
      421 ttcctccttc taccgtcgtt ttatgcgtca aattttacag taaattttgc ttcatcaaaa
      481 gaaataacct ttaacaagta tagtaatttt acattttaca atgttacaaa atataatttt
      541 ttataattag ttaaaatcac taaaacgctt ttatacacta tcaaatcagc atttataaaa
      601 atatgaaccg atatcctaaa atgttaataa tattacaaga taataacaaa ccacacaaag
      661 ctacttattt ttgataatat ggaaatcgta atataaaacg aaaacttaat ttactatata
      721 aattgtctta ataattttta aaagtagtaa aacataattt taaggaggag tccctttgaa
      781 aaaattagca tttgcaataa cagcaacatc tggtgcagct gcatttttaa cgcatcatga
      841 tgcacaagct tctacacaac atacagtaca atctggtgaa tcattatgga gtattgctca
      901 aaaatacaac acttcagtag agagtattaa acaaaataac caattagata acaacttggt
      961 attccctggt caagttatct cagtaggtgg aagtgatgca caaaatacgt caaacacttc
     1021 tccacaagct ggttcagcat catctcatac tgtacaagct ggtgaatcat taaatatcat
     1081 tgctagcaga tatggtgttt cagttgatca attaatggca gccaataact tacgtggtta
     1141 tttaattatg cctaaccaaa cattacaaat tcctaatggt ggatcaggtg gtacaacacc
     1201 aacagctaca acaggtagca atggcaatgc atcatctttt aatcaccaaa atttatacac
     1261 tgctggtcaa tgtacatggt acgtatttga ccgtcgtgct caagctggta gtccaattag
     1321 cacatattgg tcagacgcta agtattgggc tggtaacgca gctaatgatg gttaccaagt
     1381 aaacaacaca ccatcagttg gttcaattat gcaaagcaca cctggtccat atggtcatgt
     1441 tgcttatgtt gaacgtgtca atggtgatgg tagtatcttg atttctgaaa tgaattacac
     1501 atatggtcca tacaatatga actaccgtac aattccagct tcagaagttt ctagctatgc
     1561 attcatccat taattaaata aattgtactg atatatacta gcaattcaca tcatgtgaga
     1621 ttgctagttt tttatttttg aaaaaaattt tcattttggt acaaaaaatt atctcaccct
     1681 tccctatcat acatatttat attttgtatg aatggtagtt aggtaaaaat taacaaccta
     1741 cctatttgat taaattaaat gcataatata ttaaaaatca aaataatgca actaagttga
     1801 ctatataatg atttaatttg ttataccagt attttacgct ttttcgtcta catatacaaa
     1861 tttatattaa ataaagccca atacaattta ggttaattaa acaagttgat aactatttaa
     1921 ttattccttc attgaagaat ataaactatt aaatcattat tttgctctta catatatttt
     1981 aatgacctaa ctgattatgt tccatggaat acatttataa tatagcctcc taattaaaat
     2041 gctttgtctt ggtcattcta cgtaaattct ataaaatatg ttatctactt acataaaaaa
     2101 ctgaacttca ataccaccat atgtttgtga tactgaagtt cagtttagtt ttattttcaa
     2161 ttagaaaaat aagttaagta tatagaatag taaacctgct aacaatgctg aaataggtaa
     2221 tgtaatcacc catgtaatga tcattcgttg cgcagtgctc cattttacac ctttagctcg
     2281 gttagaagca ccaacaccta agattgatga tgacacaacg tgagttgttg ataatgggaa
     2341 atgtagcgat gatgcaacaa aaattgttaa tgcagatgat aaatcggccg cagcaccatt
     2401 tgctggacgt attttcataa tattaccacc tacagttttg ataattttcc agccaccaat
     2461 tgcagtacca agccccattg ctgtcgcaca ggcaaatttt acccataact gtggttcaac
     2521 actgccatca ttctgtacat tagcgacaat caatgccaac gtaataatac ccattgattt
     2581 ttgcgcatca ttcgtaccgt gagagaatga ttgtaacgct gctgtgaaaa tttggaaaaa
     2641 tctaaagtta cgattcgctc ttgttaaatt tgcattttta aagataactt taaaaattga
     2701 atacatcaag aaaccaacac aaaatgcgat aatcggtgaa acgattaata caataataat
     2761 ttttgtgaaa ccttggtaat gtaacactcc aaatgagcct tcagatgcga ttgctgcacc
     2821 cgcaattgaa cctataagtg catgtgaaga cgaacttgga attccgtaaa accaagtagc
     2881 taaattccaa ataatagccg caagtattgc agctaacaca acaactaatc cattttccaa
     2941 tttaaatgga tcgacaatgt ctttagtaat ggtgcctgca acgcccgtaa atgttaaagc
     3001 acctataaag ttcatcactg ctgccattaa aattgccgtt ttaggcgtta acgctctagt
     3061 agatacagca gtagctactg cattggctgt atcatggaat ccattgataa agtcaaatat
     3121 cagcgagaaa ataactacag ctatagtgac gatgattata tatgaca
//
LOCUS       DAMQYT010000232         3144 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMQYT010000232 DAMQYT010000000
VERSION     DAMQYT010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.239"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            140..775
                     /locus_tag="PGA33_002492"
                     /pseudo
     CDS             140..775
                     /locus_tag="PGA33_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            772..1302
                     /gene="sat4"
                     /locus_tag="PGA33_002493"
     CDS             772..1302
                     /gene="sat4"
                     /locus_tag="PGA33_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HDG6474263.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFEKAVFWYLRF"
     gene            1395..2189
                     /locus_tag="PGA33_002494"
                     /note="aph(3')-IIIa"
     CDS             1395..2189
                     /locus_tag="PGA33_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDG6474264.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2465..3036
                     /locus_tag="PGA33_002495"
                     /pseudo
     CDS             2465..3036
                     /locus_tag="PGA33_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         1055 a          495 c          719 g          875 t
ORIGIN      
        1 cgaacactga catgatatat tagtgattag ctatattttt tactttgcaa cagaaccatc
       61 atttttatta aaatactctt ttgtaaaatc tttctgctct tcaatgtatt taatttcttt
      121 acctctaaat ctattatagc tatttgatga ttacaataaa attgatctta ccttattgcc
      181 cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc taattgataa
      241 agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg taagaaagcc
      301 aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac cttatgttat
      361 taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc agattgttcg
      421 ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct ttaaatgaag
      481 tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt gggagaaact
      541 tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat ttctatgcca
      601 tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg cctatccgga
      661 gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca ctggtaaaac
      721 cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac agtgattaca
      781 gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt tgaggtgata
      841 ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt actctatgaa
      901 gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga ggcagattgc
      961 cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga cgataaatgc
     1021 gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga agatatcgcc
     1081 gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat atctatagaa
     1141 tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa taaccttata
     1201 gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac tatgttatac
     1261 gccaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
     1321 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
     1381 gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
     1441 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
     1501 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
     1561 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
     1621 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
     1681 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
     1741 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
     1801 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
     1861 gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa
     1921 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
     1981 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
     2041 acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac
     2101 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
     2161 attatatttt actggatgaa ttgttttagt acctagattt agatgtctaa aaagctttaa
     2221 ctacaagctt tttagacatc taatcttttc tgaagtacat ccgcaactgt ccatactctg
     2281 atgttttata tcttttctaa aagttcgcta gatagagttc tatattagag atgtaaagag
     2341 ttttggtgaa gaggtttgtg agtatggctt ttataaccag ggtgcaatga gaagcactaa
     2401 cagcagctag cttaagaacg cataggatat actttttatg gaagtcacag aggaggaata
     2461 aaatttgaac aggattaaca gagtaacctc tattcttatt cagctgcaat caaaaaaata
     2521 attcctgcca aagaaattgc acagcgattt aatataagct taaggacagt ttacagagat
     2581 atccggacac ttgaagaagc cggaatacca attggatctg aggccggaaa aggatatttt
     2641 cttgtagagg gcttcctact tctgccggta atgtttacag cggcggaagt gggtgcattg
     2701 attacagcag ggaaattttt aaattgccat ggagatgaat catttataaa ggattttgat
     2761 tcagctatgt ataaaatcaa atctattttg aagcatggtg aaaaaaacta tgcacaggag
     2821 ttggagaaca gtattaacgt gtacagcaca tctggacaga aaaatacatt ggcggataac
     2881 gttattgcag caattcagac tgcaatctgc aataaaaggg taatatcaat tcaatatcct
     2941 gcatcaggag ggcaagaacc ggaaagcaga atgatagagc ctatatcact ggggttttat
     3001 gaacagaact ggtacttaat cggttttgca ggctaagaaa tgaatatagg aattttcgtg
     3061 tggatcggat taaaagcatt tgcatagaag aagagctctg tcaaagccat gatggctctt
     3121 tggaacaaat attaaaacaa atgc
//
LOCUS       DAMQYT010000233         3128 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMQYT010000233 DAMQYT010000000
VERSION     DAMQYT010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.240"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            564..1145
                     /locus_tag="PGA33_002496"
     CDS             564..1145
                     /locus_tag="PGA33_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474265.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIA
                     DKNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDI
                     ENKKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVA
                     KKLAKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
     gene            complement(1471..1659)
                     /locus_tag="PGA33_002497"
     CDS             complement(1471..1659)
                     /locus_tag="PGA33_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474266.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILM
                     GINAIYLVVKVMNFKKNN"
     gene            complement(2242..>3128)
                     /locus_tag="PGA33_002498"
     CDS             complement(2242..>3128)
                     /locus_tag="PGA33_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HDG6474267.1"
                     /translation="VSYADILEILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCK
                     SKLCPVCNWRRAMKNSYQAQKVIEKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHL
                     TKAFDRLSRYKKVKQNLVGFMRSTEVTVNKNDGSYNQHMHVLLCVENAYFRKKENYIT
                     QEEWVNLWQRALQVDYRPVANVKAIKPNRKGDKDIESAIKETSKYSVKSSDFLTDDDE
                     KNQEIVSDLEKGLYRKRMLSYGGLLKQKHKILNLDDVEDGNLINASDEDKTTDEEEKA
                     HSITAIWNFEKQNYYLRH"
BASE COUNT         1140 a          520 c          379 g         1089 t
ORIGIN      
        1 tacattatct attttctttt ctctccatgg gcgttgtttc ccacttttcg tcatatcttt
       61 taacgtttta ttatcttgat tttcggttat acttctagta gtattatatt gcatataaaa
      121 agacccccag tattatttaa gattcgacac cttaataata taactggggt tttttattgt
      181 caaattttcc tttcacgcac aaaaacacgt ttaaacactg taatatcaac agttacagct
      241 atatataaaa tcgagttatt tctatatagt atcaagacaa gaagaaactc gttcacactc
      301 gtttcaaaaa acaacctatt ccttcttctt tttatgaccc tctaaaccta aataaattaa
      361 tgtgaaaacc aatgaataaa taaaactttc taaaaagtca aaaggattat caaataaaat
      421 cgaattaaca aaattaaaaa ttaaaaatac agataataaa ataatcaaaa agtaaaaaaa
      481 cttattcata ttaaactcct ttgtttttat atgttaatat tatattgaat tcatttaaat
      541 aacaaaacaa gggggcattt acaatgaatg caattcaaaa cagtatatca ctaagaaaga
      601 ttttagtagt atctttaagt attttaacca tactttcaat ggtcttggat tttaatttca
      661 aagaagctca agcccaaaac aaaaataaca ttgcagataa aaacgttgaa actttaaacg
      721 aaaaagaaat tgaaaaagaa ctaaaataca tatatggcaa aattattatt ttagataaag
      781 acggaacagc taaagatgtt aatctagaaa atgccaaatc tcgatatgga tatgttcctg
      841 aaggattcca aaaactaaaa aacgacatag agaataaaaa gaaaactagt tcaatatcac
      901 ctagagcagt tggtgggaac tacaaaaata gcggtgactg tttctattct gaagttttaa
      961 actcatatgg agaactttta actggaaaca taatcacagc agtttttgaa gatgttaaag
     1021 ccaaaaactt aaagggcgta gcaaaaaaat tagctaaaat tgggataaaa ggaaacctag
     1081 ctggaatcgc agctactatg gtttcgaagg atgtacaatg taatcttaaa tacgggttac
     1141 tttagatact atttattcca attgctttat tgacgttgag cctcggaacc cttaacaatc
     1201 ccaaaacttg tcgaatggtc ggcttaatag ctcacgctat gccgacattc gtctgcaagt
     1261 ttagttaagg gttcttctca acatcaataa attttctcgg cataaatgcg tcgtctattt
     1321 tttattatct tcatgataat catatttttc ttccatctcg tcaaaagcat catctttata
     1381 gaaaaacaaa aagtaagcaa caacaccaat aggaataaaa aaacagtgtg aaaacaagaa
     1441 gaaactcgtt tcaaaatccc ctcaaatatt ctaattattc tttttgaaat tcataacctt
     1501 aacaaccaaa taaatagcat ttattcccat caaaataaca tacggaatta atatatataa
     1561 attttccaaa tgagatataa aaattaatcc aagtaaaaag aaaacattta aaatagttgc
     1621 tataacgtaa ccttcatcta acactgtgtt tttattcaaa ataaacactc cttttttaaa
     1681 aaactttatc acaagactta ctcaaattat aaataccttt attttgagtt ttaagggggt
     1741 attttaaagg tttagggggt atcatatagg tttttaactt aaaaatatat acaagctctt
     1801 aaaacgcaaa tatgagccaa ataaatatat ggtaatttca caaacaaaaa tttcagcaaa
     1861 accagtgact aattttttaa cactgcccat ttacatgcaa attaaaaatt agcataaaat
     1921 cctttaaaac ttccacatgt ttttctaaat tcctgcaggg cgttttttat caaaaatcga
     1981 gaatttttaa tttttctcta aaaactccgt aagagcataa ggagaatttg ctatcattca
     2041 aaaatgatag gtctggcaaa gccagaaatt ccaggggttt taaggtattt taaagtgata
     2101 tcatacaaag ctgtactgtc ttatttttgt gacacaatat attgcgtttg tcttattttt
     2161 gtgacactac atacagcatt tgtcacaaaa ataagacagt acttttttat aaaaaatcaa
     2221 caaaaaagac attagtcaac actaatgtct taaataataa ttttgctttt cgaaattcca
     2281 aattgcggta attgaatgtg ctttttcttc ttcgtctgtt gttttatctt catcacttgc
     2341 attaatcaaa ttaccatctt cgacatcgtc taagtttaaa attttatgtt tttgtttaag
     2401 caatccacca taacttaaca tacgttttcg atacaaacct ttttctaaat cacttacaat
     2461 ttcttgattt ttttcatcat catcagttaa aaaatcagat gatttaaccg aatattttga
     2521 ggtctctttg attgccgatt caatgtcttt atcgcctttt ctattcggtt tgatcgcttt
     2581 aacattagca acaggtcgat agtcaacttg taatgctctt tgccataaat taacccattc
     2641 ttcttgagtt atataattct ctttttttct aaaatatgca ttttcaacac ataacaaaac
     2701 atgcatgtgc tgattataac taccgtcatt tttattaacg gtaacttctg ttgaacgcat
     2761 aaatccaaca agattttgtt taaccttttt atatctactc aacctatcaa atgctttagt
     2821 tagatgcttc aaactttgtt ctaaagtatc tccatctatc gcattttttg ttgaaagtgt
     2881 taaaaacaac caacgtgctt ttggcttttc cttaattact ttttcaatca ctttttgagc
     2941 ttgataacta tttttcatag cacgtctcca attacaaacc ggacataatt ttgatttaca
     3001 aaaccatgtc ttatgtaact tcaaatagcc ttcatcagtt ggcttaaatt ctaaaatatt
     3061 accacattgt tttacattaa aagccttttt gatttttaaa atttctagta tatctgcata
     3121 gcttacat
//
LOCUS       DAMQYT010000234         3119 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMQYT010000234 DAMQYT010000000
VERSION     DAMQYT010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.241"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            76..801
                     /locus_tag="PGA33_002499"
     CDS             76..801
                     /locus_tag="PGA33_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HDG6474268.1"
                     /translation="MKKNIMNKLVLSTALLLLETTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHFSLSGKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     EFYKGNKPVLTLKELDFRIRQTLIKNKKLYNGEFNKGQIKITADGNNYTIDLSKKLKL
                     TDTNRYVKNPRNAEIEVILEKSN"
     gene            896..1621
                     /locus_tag="PGA33_002500"
     CDS             896..1621
                     /locus_tag="PGA33_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HDG6474269.1"
                     /translation="MNNNITKKIILSTTLLLLGTASTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKPVLTLKELDFRIRQTLIKSKKLYNNSYNKGQIKITGADNNYTIDLSKRLPS
                     TDANRYVKKPQNAKIEVILEKSN"
     gene            1729..2445
                     /locus_tag="PGA33_002501"
     CDS             1729..2445
                     /locus_tag="PGA33_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HDG6474270.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KDRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYNENRNKGKLKITGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
     gene            complement(2890..3114)
                     /locus_tag="PGA33_002502"
     CDS             complement(2890..3114)
                     /locus_tag="PGA33_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474271.1"
                     /translation="MLIQERGLKMTELNNIINSLQSLFESESGYKISKNSGVPYQTVQ
                     DLRNGKTKLEDARFRTIIKLYSYYVSLKEH"
BASE COUNT         1169 a          477 c          461 g         1012 t
ORIGIN      
        1 tttagctttt atttaattcc gaatcggtgt aatagcttat atactttggg taattcacgc
       61 aaaggagatt ttcatatgaa aaagaacatc atgaataaat tagttttatc aacagcattg
      121 ttacttttag aaactacatc aacacaactt cctaaaacac caatcagttt ttcatctgaa
      181 gcaaaagcct ataatatcag tgaaaacgag actaatatca atgaactaat caaatattac
      241 actcagccgc atttttcatt atctggaaaa tggttatggc aaaagcccaa tggtagcatt
      301 catgcaacat tgcaaacgtg ggtttggtat agtcatattc aagtgtttgg atccgagagt
      361 tggggaaaca ttaatcagtt aagaaataaa tacgttgata tatttggaac taaagatgag
      421 gacacagttg aaggttactg gacttatgat gaaacattta ctggtggtgt tacgccagca
      481 gctacttcat ctgataagcc ttatagacta tttttaaaat atagtgataa acaacaaact
      541 atcatcggtg gacatgaatt ttacaaagga aataaaccag tattaacttt aaaagaatta
      601 gatttccgta ttcgtcaaac attaataaaa aataaaaagt tatataacgg agaatttaat
      661 aaaggtcaaa ttaagataac tgctgatgga aataattaca cgattgattt aagtaaaaag
      721 ttaaaattaa ctgacacaaa ccgttatgtt aaaaatcctc gtaatgcaga aattgaagtc
      781 atactcgaaa aatctaacta acctattacc ttttgtaaat gcggataatt tcaattatct
      841 aattaacccc tttttataat taaacattcc aacaatactc aaaggagaaa ttcgaatgaa
      901 caataacatc acgaaaaaaa ttattttatc aacaacattg ttactattag gtacagcatc
      961 tacacaattt cctaatacac ctatcaattc ttcatctgaa gcgaaagctt attatataaa
     1021 tcaaaacgaa actaacgtta atgagttaac taaatattac tcgcaaaaat atttaacctt
     1081 ctctaacagt acgttatggc aaaaagataa cggtacgatt catgcaacgt tgttacagtt
     1141 ttcttggtat agtcatattc aagtttatgg acctgaaagt tggggcaata tcaaccaatt
     1201 aagaaataaa agcgttgata tttttggcat aaaagaccaa gaaaccattg attcttttgc
     1261 attatctcaa gaaacgttta ctggtggtgt tactcctgca gcaacatcta acgataaaca
     1321 ctataaactg aatgtaacat ataaagataa agcagaaacg tttactggcg gatttccagt
     1381 ttatgaaggc aataagcctg ttttaacttt aaaagaatta gattttcgta ttcgtcaaac
     1441 attaattaaa agtaaaaaat tatataataa ttcttataat aaaggacaaa ttaaaataac
     1501 aggtgcagac aataactaca caatagattt aagtaaaagg ttgccatcaa ctgatgcaaa
     1561 tagatatgtt aaaaaacctc aaaatgcaaa aattgaagtt atcctcgaaa aatcaaacta
     1621 acaataataa tggagttaat aaaaataatc gcaaatacta tattgacttc gctcacattt
     1681 aaatttctta ttcctcgtat catgattcct ctgaaaggag atgttctaat gagtaagaac
     1741 atcacgaaaa atataatttt aacgacaaca ttattactat taggtactgt attacctcaa
     1801 aatcaaaaac cagtatttag tttttactct gaagctaaag cttatagcat tggtcaagat
     1861 gaaactaaca tcaatgaatt aattaaatat tacacacagc ctcatttttc attttcaaat
     1921 aaatggctat atcaatatga taatggaaac atttatgttg aacttaagag atattcatgg
     1981 tcagcacata tatctttatg gggcgctgaa agttggggaa atattaatca gttaaaagat
     2041 cgttacgtag atgtgtttgg actaaaagac aaagatactg atcagttatg gtggtcttat
     2101 agagagacat ttacaggtgg cgttacacca gccgcaaaac cttctgataa aacttataat
     2161 ctttttgtgc aatacaaaga taaactacaa acgattattg gtgcgcataa aatataccaa
     2221 ggcaataaac cagtattaac attgaaagaa atcgatttcc gtgcacgaga agcgttaata
     2281 aaaaataaaa tattatataa cgaaaatcgt aataaaggta agcttaagat caccggtggc
     2341 ggtaataact acactattga tttaagcaaa agattacatt cagatctagc aaatgtttat
     2401 gttaaaaatc ctaataaaat aactgttgac gtcctctttg attagtatat gaaggtgact
     2461 tatacttcat gcactttaat tccaaatcag attatttaaa tgataatttt taaagtgtat
     2521 gatgtatata ataggtaaaa ttttctatat atttaaatgg aattgggagt aggaatgtga
     2581 cagaaatagt attttataaa atttatttcg ttgtcactcc ccaacttgca cattattgta
     2641 agctgacttt tcgtcagctt ctgtgttggg gcccacccca acttgcattg tctgtagaat
     2701 ttctttttga aattctctat gttggggccc cgcctataat tgaaaaatgc ttgttacatg
     2761 ggcattttca ttcggtcaac tactaccaat ataatattgt agagcctaag acattgattt
     2821 attatgtctt aggctctatt ccttcattta atgattaaat tattatagca atactttatt
     2881 gtcccatgat tagtgttctt ttaatgagac atagtaacta taaagtttaa taatcgttct
     2941 aaatctagca tcttctagtt tggttttccc atttcttaaa tcttgtactg tttgatatgg
     3001 aactcctgaa tttttcgaaa ttttatagcc tgattcagac tcaaacaaag attgaagaga
     3061 gtttataata ttattcaact ctgtcatttt taatcccctt tcttgaatta acaatatac
//
LOCUS       DAMQYT010000235         3112 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMQYT010000235 DAMQYT010000000
VERSION     DAMQYT010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.242"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..199
                     /locus_tag="PGA33_002503"
     CDS             <1..199
                     /locus_tag="PGA33_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HDG6474272.1"
                     /translation="EKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYEL
                     ASKTFKKNVVAPEPYKVLEFI"
     gene            209..1582
                     /locus_tag="PGA33_002504"
     CDS             209..1582
                     /locus_tag="PGA33_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDG6474273.1"
                     /translation="MKIIKRAIISLIILSLLISITMSNASASEELYYSVEYKNTATFN
                     KLVKKKSLNVVYNIPELHVAQIKMTKMHANALANYKNDIKYINATCSTCITSEKTIDR
                     TSNESLFSRQWDMNKITNNGASYDDLPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKN
                     LVPLNGFRGTEPEETGDVHDVNDRKGHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGS
                     KKTELLWVSKAIVQAANDGNQVINISVGSYIILDKNDHQTFRKDEKVEYDALQKAINY
                     AKKKKSIVVAAAGNDGIDVNDKQKLKLQREYQGNGEVKDVPASMDNVVTVGSTDQKSN
                     LSEFSNFGMNYTDIAAPGGSFAYLNQFGVDKWMNEGYMHKENILTTANNGRYIYQAGT
                     SLATPKVSGALALIIDKYHLEKHPDKAIELLYQHGTSKNNKPFSRYGHGELDVYKALN
                     VANQKAS"
     gene            1605..2297
                     /locus_tag="PGA33_002505"
     CDS             1605..2297
                     /locus_tag="PGA33_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding subunit"
                     /protein_id="HDG6474274.1"
                     /translation="MAKLVTENISKRFKNQDVLKHINITLENNEVYGLLGINGAGKTT
                     LMKIICGILQQDSGEIKLDNRPMTRNDLHKVGSLIETPATYNHLSAQDNLKIVCLNES
                     VDFSEINSVLSLVNLNVDKKKKVKDFSLGMKQRLGIAMALIKKPEILVLDEPSNGLDP
                     YGIQELRELLKLLTEQGTSIIISSHILSEIQVLADHIGIIHEGELKYQQRNNKDENLE
                     EIFFKITKGDYK"
     gene            2294..3055
                     /locus_tag="PGA33_002506"
     CDS             2294..3055
                     /locus_tag="PGA33_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDG6474275.1"
                     /translation="MIHLKIEGIKFKNSFSMYVLLISPLVFLCFAIFTVLFAKSNTGT
                     ANSVSPYITLLFNIWPIAFIPIVLCMACNSLFKIEMRNKSFNYYLSNNWSITKEIRAK
                     IFILSIAFLVHCFLVFIIAYIGDLIINPHPVNAMLLLVTILLMYVVSLPLIPLNFLLT
                     RYFGVFVSILINLVLSVICVLFLTLKSLFWVLPWGIMQRIPLITLGILPNGLVVNHNS
                     KYFNDLNALYISIIVSIIIFAIVTFLNNKKSWRLK"
BASE COUNT         1155 a          409 c          549 g          999 t
ORIGIN      
        1 tgaaaaactt tctatatttc caaatatgaa tttacgaatg tgggaaaatc cagttactca
       61 aaataatatt cgattattaa aagattatgg tgtatcaata tatccagcaa atatttcaga
      121 aagttatgaa ttagcgtcaa aaacatttaa aaagaatgtt gtcgcacctg aaccatataa
      181 agttctggaa ttcatttgag attagaatat gaaaatcata aagcgcgcta ttattagttt
      241 aataatattg agtcttttaa tatctattac aatgagtaat gcatcagcgt cagaagaact
      301 atattacagt gttgaataca aaaatacagc aacatttaac aagttagtta aaaagaagtc
      361 cttaaacgtt gtctataata taccggaatt acatgtggca cagattaaaa tgacgaaaat
      421 gcatgctaat gctttagcaa actataaaaa tgatattaaa tatatcaatg ccacatgttc
      481 aacttgtatt actagcgaga aaacaataga cagaacatca aatgagtcat tattttcaag
      541 acaatgggat atgaataaaa taaccaataa tggtgcatcg tatgatgatt tgccaaaaca
      601 tgctaacacc aaaatagcaa tcatagatac aggtgtgatg aaaaaccatg acgatttgaa
      661 aaataatttc tcgactgatt ctaaaaattt agtaccttta aacggtttta gaggtactga
      721 accggaggaa acaggtgatg ttcacgatgt caatgatagg aaaggacatg gtacgatggt
      781 gtcgggtcaa acgagtgcta atggtaagtt aataggtgtt gcaccgaata acaaatttac
      841 aatgtatcgc gtgtttggta gtaaaaaaac agaactgctt tgggtatcaa aagcgattgt
      901 tcaagctgca aatgatggaa atcaagtcat taatattagt gttggtagtt atattatttt
      961 ggacaaaaat gaccatcaaa catttagaaa agatgaaaaa gtagaatacg atgcgttaca
     1021 gaaagcaatc aattacgcca agaagaaaaa atctatcgtt gttgctgcag ctggtaatga
     1081 tggtattgat gtcaatgaca aacagaaact aaaattacag cgtgaatatc aaggtaatgg
     1141 cgaagtgaaa gatgttcctg catctatgga caatgtcgtt acagtaggat ctacagatca
     1201 aaagagtaat ctatctgagt tttccaattt tggtatgaat tatacagata ttgctgcgcc
     1261 cggaggatca tttgcttatt taaatcaatt cggtgtggat aaatggatga atgaagggta
     1321 tatgcataag gagaacattt taactactgc caataacgga agatatattt atcaagctgg
     1381 aacttcatta gccacaccta aagtttcggg agcactagct ttaatcattg ataaatatca
     1441 tcttgaaaaa catccagata aagcgattga attgttatat cagcatggga catctaagaa
     1501 taataaacca tttagtagat atgggcatgg tgagcttgat gtgtataaag cattaaatgt
     1561 agcaaatcaa aaagcaagtt aataaatcaa aggagttttt gattatggca aaattagtta
     1621 ctgaaaacat ttcgaagcgg tttaaaaatc aagatgtatt aaagcatatt aatatcactt
     1681 tagaaaataa cgaagtttat ggattacttg gtattaatgg agccggtaaa acgacactta
     1741 tgaaaattat atgtggcata cttcaacaag attcagggga aattaaatta gataatagac
     1801 caatgacacg aaatgatttg cacaaagttg gttcgcttat tgaaacacct gcgacatata
     1861 atcatttaag tgcacaagat aatttgaaaa ttgtgtgttt aaatgaaagc gttgatttca
     1921 gcgaaattaa tagtgtttta agcttagtca atttaaatgt cgataaaaag aaaaaggtta
     1981 aggacttttc tttaggtatg aaacaaagac ttggaattgc aatggcatta attaaaaagc
     2041 cagaaatttt agtattagac gaaccatcta atggtttaga cccatatgga atccaagaac
     2101 ttagagaact tctaaaatta ttaacagaac aaggtactag tattattatt tcaagtcaca
     2161 ttttatctga aatccaagtt ttagcagatc atatcggtat tattcatgag ggtgagctaa
     2221 aatatcagca aagaaataac aaagatgaaa acttagaaga gatattcttc aaaataacga
     2281 aaggtgatta caaatgatac atttaaagat agaaggtatc aaatttaaaa attctttcag
     2341 tatgtatgtt ttattaataa gtccgctggt atttctttgt tttgctattt tcacagtctt
     2401 attcgccaaa agtaatacgg gaacagcgaa tagtgtgtca ccatatataa ctttactatt
     2461 taatatttgg ccaattgctt tcatcccgat tgtattatgt atggcttgta attcgttatt
     2521 taaaattgaa atgagaaata aatcatttaa ttattactta agtaataatt ggtcgattac
     2581 aaaagaaata agagcaaaga ttttcatttt atcaatagca tttttggtac attgcttttt
     2641 agtatttatt attgcttata taggtgacct aattattaat ccacatccgg ttaatgctat
     2701 gttgttattg gtgacaatat tgttgatgta tgtagtatct ctaccattga taccgctcaa
     2761 ctttttatta actcgatact ttggtgtgtt cgtatcaata ttaataaact tagtattatc
     2821 agtcatttgt gtcttgtttt taacattgaa gagtttattt tgggtgttgc cgtgggggat
     2881 aatgcagaga atcccgctta ttacgcttgg tatactacct aatggtttag ttgtaaacca
     2941 taattcaaaa tactttaatg atctcaatgc cttatatatt tcgattattg ttagcatcat
     3001 tattttcgcg atagtaacat ttttaaataa taagaaaagt tggcgattaa aatgataatt
     3061 aacgaattaa agtcatgtaa gttgaaattt tctaagcaag cgctcacatt tg
//
LOCUS       DAMQYT010000236         3075 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMQYT010000236 DAMQYT010000000
VERSION     DAMQYT010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.243"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            140..1036
                     /gene="xerC"
                     /locus_tag="PGA33_002507"
     CDS             140..1036
                     /gene="xerC"
                     /locus_tag="PGA33_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDG6474276.1"
                     /translation="MNHIQDAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLEL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKNLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            1033..1578
                     /gene="hslV"
                     /locus_tag="PGA33_002508"
     CDS             1033..1578
                     /gene="hslV"
                     /locus_tag="PGA33_002508"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDG6474277.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            1644..3047
                     /gene="hslU"
                     /locus_tag="PGA33_002509"
     CDS             1644..3047
                     /gene="hslU"
                     /locus_tag="PGA33_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDG6474278.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
BASE COUNT         1147 a          444 c          616 g          868 t
ORIGIN      
        1 aaaaaagagg tattctaatt aacaggtttt ccgattagtt acaattattt aattctcaaa
       61 agatttagaa ttgattatca aattactgta agccctttgc tgtatatgct acaattctta
      121 ttgatggagg gtaaatgtat tgaatcatat tcaagatgcg tttttaaata cattgaaagt
      181 tgaacggaat ttttcggaac acacattgaa atcatatcaa gatgacttaa ttcagtttaa
      241 tcaattttta gaacaagaac atttagagtt gaatactttt gaatacagag atgctagaaa
      301 ttatttgagc tatttatatt caaatcattt gaaaagaaca tctgtttctc gtaaaatctc
      361 aacgttaaga actttctatg aatattggat gacgcttgat gagaacatta ttaatccatt
      421 tgttcaatta gtacatccga aaaaagaaaa atatcttccg caattctttt acgaagaaga
      481 aatggaagcg ttattcaaaa ctgtagaaga ggacacttca aaaaatttac gggatcgagt
      541 tattcttgaa ttgttgtatg ctacaggcat ccgtgtttcg gaattagtaa atattaaaaa
      601 acaagatata gatttttacg cgaatggtgt taccgtatta ggaaaaggga gcaaagagcg
      661 ctttgtaccg tttggtgctt attgtagaca aagcatcgaa aattatttag aacatttcaa
      721 accaattcag tcatgcaatc atgattttct tattgtaaat atgaagggtg aagcaatcac
      781 tgaacgcggt gtacgatatg ttttaaatga tattgttaaa cgaacagcag gcgtaagtga
      841 gattcatccc cacaagctca gacatacatt tgcaacgcat ttattgaatc aaggtgcaga
      901 cctaagaaca gtacaatcgt tattaggtca tgttaatttg tcaacaactg gtaaatatac
      961 acacgtatct aaccaacaat taagaaaagt gtatctaaat gcacatcctc gagcgaaaaa
     1021 ggagaatgaa acatgagtaa tacaacatta catgcaacaa caatttatgc tgtaagacat
     1081 aatgggaaag cagctatggc tggagatggg caagtaacgc ttggtcaaca agtcatcatg
     1141 aaacaaacgg caagaaaagt gcgacgttta tatgaaggta aagtgttagc tggtttcgca
     1201 ggtagtgtag cagatgcgtt tacgttattt gaaaaattcg aaacaaaatt acaacagttt
     1261 agtggtaact tagaaagagc tgctgttgaa ttggcacaag aatggcgagg cgataaacaa
     1321 ttacgtcaat tagaagctat gctaattgta atggataaag atgctatttt agttgtcagt
     1381 ggaactggcg aagttattgc tccagatgat gaccttatcg ctattggatc aggaggcaac
     1441 tacgcattaa gcgcaggacg tgcattgaaa cgccatgcat cgcatttgtc tgctgaagaa
     1501 atggcatatg agagcttgaa agtagcggct gatatttgtg tctttaccaa cgataatatt
     1561 gttgtcgaaa cactataata atcagagcac gataaataat tacgagcaat taattttagt
     1621 taaaagacgg aggaatgaaa ttaatggata cagctggaat aagattaact ccaaaagaaa
     1681 tcgtatctaa attaaatgaa tacatcgttg gacaaaatga tgctaaacgt aaagtggcaa
     1741 ttgccctacg taatcgatac agaagaagtt tattagatga ggaatcaaag caagaaattt
     1801 cacctaaaaa tattttgatg attggaccaa ccggcgttgg taaaactgaa attgcaagaa
     1861 gaatggccaa agttgtcggc gcgccattta taaaagtaga agctactaaa tttactgagg
     1921 taggttatgt aggacgagat gttgaaagta tggttagaga tcttgttgat gtttcagtaa
     1981 gattagtcaa ggcgcagaaa aaatcattgg tacaagatga agcaacagct aaggccaatg
     2041 aaaaacttgt taagttatta gttccaagta tgaaaaagaa agcgtctcaa acgaataatc
     2101 ctttagagtc acttttcgga ggtgcaattc caaatttcgg acaaaataac gaagatgaag
     2161 aagaaccacc tactgaggaa attaaaacaa aacgttctga aattaagaga cagctagaag
     2221 aaggcaaact tgaaaaagaa aaggtaagaa ttaaagtcga acaagatcct ggtgctttag
     2281 gtatgctagg tacaaatcaa aatcagcaaa tgcaagagat gatgaatcaa ttaatgccta
     2341 aaaagaaagt tgagcgagaa gttgctgttg agacggcaag gaaaatctta gctgatagtt
     2401 atgcggatga actaattgat caagaaagcg ctaaccaaga agcgcttgaa ttagcagaac
     2461 aaatgggtat catctttata gatgaaatcg acaaagttgc gacgaataat cataatagtg
     2521 gtcaagatgt ctcaagacaa ggtgttcaaa gagatatttt acctatactt gaaggtagcg
     2581 ttattcaaac caaatatggt actgtgaata ctgaacatat gctgtttata ggtgctggag
     2641 ctttccatgt atctaagccg agtgacttga taccagaatt gcaaggtcgt tttccgatta
     2701 gagttgaact tgatagttta tcggtagaag attttgtaag aattttgaca gaaccaaaat
     2761 tgtcattaat taaacaatat gaagcattgc ttcaaacaga agaagttact gtaaacttta
     2821 ccgatgaagc aattactcgc ttagctgaga ttgcttatca agtaaatcaa gatacagaca
     2881 acattggtgc acgtcgactt catacaattt tagaaaagat gctagaagat ttatcattcg
     2941 aagcaccaag tatgccgaat gcagttgtag atattacccc acaatatgtt gatgataaat
     3001 taaaatcaat ttcaacaaat aaagatttaa gtgcatttat tctataaaaa tatacaaaag
     3061 gagaaaaatt catga
//
LOCUS       DAMQYT010000237         3055 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMQYT010000237 DAMQYT010000000
VERSION     DAMQYT010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.244"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            369..1604
                     /gene="arcA"
                     /locus_tag="PGA33_002510"
     CDS             369..1604
                     /gene="arcA"
                     /locus_tag="PGA33_002510"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDG6474279.1"
                     /translation="MVQGPIQVNSEIGKLKTVLLKRPGKELENLVPDHLSGLLFDDIP
                     YLKVAQEEHDKFAQTLRDEGIEVVYLEKLAAESITEPEVRENFINDILTESKKTILGH
                     ETEIKEFFSKLSDQELVNKIMAGVRKEEIQLETTHLVEYMDDRYPFYLDPMPNLYFTR
                     DPQASIGRGMTINRMYWRARRRESIFMTYILKHHPRFKDKDVPVWLDRNSPFNIEGGD
                     ELVLSKDVLAIGISERTSAQAIEKLARNIFKDANTSFKKIVAIEIPNTRTFMHLDTVL
                     TMIDYDKFTVHAAIFKEENNMNIFTIEQNDGKDDIKITRSSKLRETLAEVLEVEKVDF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNQLLRDKGIKVIEITGSEL
                     VRGRGGPRCMSQPLFREGI"
     gene            1690..>3055
                     /gene="arcD"
                     /locus_tag="PGA33_002511"
     CDS             1690..>3055
                     /gene="arcD"
                     /locus_tag="PGA33_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDG6474280.1"
                     /translation="MDENKLGKTSLIGLVIGSMIGGGAFNIISDMGGQAGGLAIIIGW
                     IITAIGMISLAFVFQNLTNERPDLDGGIYSYAQTGFGDFIGFSSAWGYWFAAFLGNVA
                     YATLLMSAVGNFFPIFKGGNTLPSIIIASILLWGVHFLILRGVETAAFINSIVTVAKL
                     IPIFLVIICMIVVFNFSTFKSGFYGMTSGSVGVFSWGDTMAQVKSTMLVTVWVFTGIE
                     GAVVFSGRAKSKKDVGTATVIGLISVLVIYFLMTVLAQGVIQQNQISKLANPSMAQVL
                     EHIVGHWGSVLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNKAPV
                     NALIITNILVQLFLISMLFTDSAYQFAFSLASSAILIPYTLSAFYQVKYTIQNKSKAN
                     LKQWIIGIIASIYTIWLVYAAGLDYLLLTMLLYIPGLLVYSYVQRDNNKHLTKLDYTL
                     FIFII"
BASE COUNT         1072 a          416 c          567 g         1000 t
ORIGIN      
        1 gaaagaaata ttaggaatta ttggaggtaa cgatactttg atgattttga cttcttcaaa
       61 tgaaatagca gaatttgttt gttatcaatt attcccttaa aaattaaatg attatattaa
      121 taggaatgat tactaataca aaaaaatgag taaaaaatct ttttgcttga ataagataag
      181 gttttaacaa tgagattttt caaattattc actattatat agtacaaaat acatattcat
      241 acaaaatttt gcattgaata aatctatata tgtgaaaacc ctccctaaac aattgtgaat
      301 taaatcacac gatgtttttt gaaagcgttt tataatgtga atgtaataaa gcaaaggagg
      361 aatttgaaat ggtacaagga cccattcaag taaatagtga aataggcaaa ttgaaaactg
      421 tgttgttaaa aagaccagga aaagaattag aaaatttagt acctgatcat ttaagtggtt
      481 tattattcga tgatattccc tacttaaaag ttgcacaaga agagcatgac aaatttgctc
      541 aaactttgag agatgaagga atcgaagtag tttatttaga aaaacttgca gcagaatcta
      601 ttactgagcc agaagtacgc gagaacttca taaacgacat attaacagaa tctaaaaaga
      661 caatattagg tcatgaaact gaaattaaag aattcttttc aaagttatct gaccaagaac
      721 ttgtaaataa aatcatggct ggcgtacgta aagaagaaat tcaacttgaa acaacccatt
      781 tagtagaata tatggatgat agatatccat tttacttaga tccaatgccc aacctttatt
      841 ttacaagaga tccccaagct tcaattggta gaggaatgac aattaacaga atgtattgga
      901 gagcacgacg tagagaatct atttttatga catatatact gaaacatcat ccaagattta
      961 aagataaaga tgtaccagta tggttagatc gtaactcacc atttaatatt gaaggtggag
     1021 atgaattagt attatcgaaa gatgttttag ctattggtat atcagaacgt acatcagctc
     1081 aagcaataga aaagttagca cgtaatattt tcaaagatgc aaacacaagt tttaaaaaaa
     1141 tcgtagctat tgaaatacct aatacacgta catttatgca cctagataca gtactaacta
     1201 tgattgacta cgataagttt acagtacatg cagcaatatt taaagaagaa aataatatga
     1261 atatatttac catagaacaa aatgatggta aggacgatat aaaaattact cgttctagca
     1321 agttacgtga aacacttgct gaagttttag aagtagaaaa agtggacttt attccaacag
     1381 gtaatggcga cgttattgat ggtgcacgtg aacaatggaa tgatggctca aacacattat
     1441 gtattcgacc aggggttgtg gtgacatacg atcgcaacta tgtatcaaac caacttttac
     1501 gcgacaaagg aattaaagtg attgaaatta ctggtagtga acttgtacgt ggacgcggag
     1561 gcccaagatg tatgagtcag ccgttattta gagaaggtat ttaaatttga aaggtggtaa
     1621 aagtcacaag taattcattc attaaatcaa ataacaagat ttttaccacc tttattaggg
     1681 gagattaata tggatgaaaa taaattaggt aaaacttcct taattggttt agtcataggc
     1741 tctatgatag gcggtggtgc attcaatatc atctcagata tgggtggcca agctggtgga
     1801 cttgcaataa ttatcggttg gataataact gctattggta tgatttctct tgctttcgta
     1861 tttcaaaatt taacaaatga gcgaccagat cttgatggag gaatttatag ttatgctcaa
     1921 acagggtttg gagattttat tggtttttca agtgcttggg gatattggtt tgcagcattt
     1981 ctaggtaatg tggcttatgc aaccctatta atgtcagctg tgggtaactt tttccctata
     2041 tttaaaggag gtaacacact tccaagtatt atcatagcat caattttatt atggggtgta
     2101 cattttttaa tacttagagg tgtagaaact gcagcgttta taaatagtat tgttacagta
     2161 gctaaattaa tacctatatt tctagttatt atatgcatga tagttgtatt caacttcagt
     2221 acttttaaat ccggttttta tggtatgact agtggaagtg ttggcgtttt tagttgggga
     2281 gatacaatgg cacaagtaaa aagtactatg ttagtaactg tatgggtatt cacagggatt
     2341 gaaggagccg ttgtcttttc tggacgtgca aagtctaaaa aggatgtagg aactgctacc
     2401 gttattggtt tgatttctgt gctagtcatt tatttcttaa tgactgtact agcccaaggt
     2461 gtcattcagc agaaccaaat ttcaaaactt gctaatccat caatggcaca agtattagaa
     2521 catattgtag gtcattgggg ttcagtgtta gttaatatag gcttaattat ctctgtttta
     2581 ggagcttggt taggatggac attactagct ggtgaattac cattcattgt agctaaagat
     2641 ggacttttcc cgaaatggtt tgctaaagaa aataagaata aagctccggt caacgcttta
     2701 attattacta atatattagt tcagttattt ttaattagta tgttgtttac agatagtgcc
     2761 tatcagtttg cgttttcact tgcatcaagt gcaatcttaa ttccatatac actcagtgct
     2821 ttttaccagg ttaaatatac tattcaaaat aaatctaaag ctaatttaaa acaatggata
     2881 ataggaatta ttgcatctat ttacacaatt tggttggttt atgcagctgg attagattat
     2941 ttactattaa cgatgttgtt atatatacct ggattactcg tatacagcta cgtacaaagg
     3001 gataataaca aacatttgac aaaattggat tatacgttat tcatattcat cattg
//
LOCUS       DAMQYT010000238         3051 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMQYT010000238 DAMQYT010000000
VERSION     DAMQYT010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.245"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            74..1249
                     /locus_tag="PGA33_002512"
     CDS             74..1249
                     /locus_tag="PGA33_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HDG6474281.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            1227..2417
                     /locus_tag="PGA33_002513"
     CDS             1227..2417
                     /locus_tag="PGA33_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDG6474282.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHILWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIVNR
                     EKAK"
     gene            complement(2530..3039)
                     /locus_tag="PGA33_002514"
     CDS             complement(2530..3039)
                     /locus_tag="PGA33_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HDG6474283.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIK
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
BASE COUNT         1006 a          492 c          491 g         1062 t
ORIGIN      
        1 ttatttcttt tataatgccg taaaataagt tacacttaaa taaaattttc aaactaacgg
       61 agggtggcta ttaatggtta ctcaaaataa aaagatattg attattactg gctcattcgg
      121 taacggtcat atgcaagtta cacagagtat cgttaatcaa cttaatgata tgaatctaga
      181 ccatttaagc gtcattgagc acgatttatt tatggaagct catccaattt tgacttctat
      241 ttgtaaaaaa tggtatatca atagctttaa atattttaga aatatgtaca aagggtttta
      301 ttacagccgc ccagataaac tagacaaatg tttttacaaa tactatggac ttaataagtt
      361 aattaattta ttgataaaag aaaagccaga tttaatatta ttaacgtttc ctacaccagt
      421 tatgtcggta ctaactgagc aatttaacat taatattcca gttgctacag tgatgacaga
      481 ctatcgctta cataaaaact ggattacgcc gtattcaaca agatattatg tggcaacaaa
      541 agaaacgaaa caagacttca tagacgtagg tattgatcct tcaacagtta aagtgacagg
      601 tattcctatt gataacaaat ttgaaacgcc tattaatcaa aagcagtggt taatagacaa
      661 caacttagat ccagataagc aaactatttt aatgtcagct ggtgcatttg gtgtatctaa
      721 aggttttgac acgatgatta ctgatatatt agcgaaaagt gcaaatgcac aagtagttat
      781 gatttgtggt aagagcaaag agctaaagcg ttctttaaca gctaagttta aatctaacga
      841 gaatgtattg attctaggtt ataccaaaca catgaatgaa tggatggcat caagtcaact
      901 tatgattacg aaacctggtg gtatcacaat aactgaaggt ttcgcccgtt gtattccaat
      961 gattttccta aatcctgcac ctggtcaaga gcttgaaaat gccctttact ttgaagaaaa
     1021 aggttttggt aaaatcgctg atactccaga agaagcaata aaaattgtag caagtttaac
     1081 taacggtaat gagcaattaa ctaacatgat tagtacaatg gaacaagata aaattaagta
     1141 tgctacacag acaatatgtc gagacttact tgatttaatc ggtcattcat cacaaccaca
     1201 agagatttat ggaaaggttc ctttatatgc aagattcttc gttaaataat tacgctaatc
     1261 ataaaaattt cattttaatg cttattatct tatttttaat ggaatttgcg agaggcatgt
     1321 atatcctaag ctatattaat ttcttgccaa cagttacatc cattgctgta gcaataacgt
     1381 ctctagcatt ttcaatacat tttattgctg atgcatctac aaactttgtt ataggatttt
     1441 tactgaaaaa attcggcaca aaaatcgttt taacgactgg atttatttta gcatttacaa
     1501 gtttattttt agttatttgg tttccagcat caccttttgt aatcatattt agtgctatga
     1561 tgctcggtat tgcagttagt cctatttggg tcattatgtt atccagtgtt gaagaagata
     1621 aacgtggcaa acaaatgggc tatgtttatt tttcatggtt gttaggccta ttggtaggta
     1681 tggtttttat gaatctactt atcaaagtgc acccaacacg ctttgccttc atgatgtctt
     1741 tagtcgtttt aattgcatgg atattatatt attttgtcga tgtaaagtta acaaattata
     1801 atactcgtcc agttaaagct caattgagac aaattgttga tgtgacaaag cgccatttat
     1861 tgttgttccc tggtattctt ttacaaggtg ccgcaattgc tgccctagtt cctatattac
     1921 caacatatgc tactaaggtt attaacgtaa gcacaattga atatactgtt gcaatcatta
     1981 ttggtggtat cggctgtgca gtttcgatgc tatttttatc gaaactaata gataaccgca
     2041 gcagaaactt tatgtatggt gttattctaa gcggatttat tttatacatg atattaattt
     2101 ttactctatc tatgattgtt aatattcaca tcttgtggat tatcgcttta gctatcggtc
     2161 taatgtatgg catcttatta ccagcatgga atacctttat ggcacgattt atcaaatcag
     2221 atgaacaaga agaaacttgg ggtgttttta atagtatcca aggattcggc tcaatgatag
     2281 ggccattatt tggcggatta attacacaat ttaccaacaa tttaaacaat acattttatt
     2341 tctcggcatt aatcttttta gtcttagctg tattttatgg aagctacttt atagtaaata
     2401 gagaaaaagc taagtaaggt taaaacgtat tttaaaataa agaaaatggt catttttatt
     2461 acattcaata aaaatgacca ttttcttatt aattcaaaca tactacgatt aacaatacta
     2521 ttatgttact taagctaatt taaacgtttc aataactttc catttatctt cgtcatcgtc
     2581 aaaacgtaac aaagttaatt catcgataat ttctttatgg tctaccccag ctaatgctac
     2641 ttgaccaaaa atatcttcga attcttggct agatagtcct tgtgctattg taaagtgtgg
     2701 cacaaaaaca tgttcagctt ctccatagaa atcttctcca ttaaagcgat taaacaattc
     2761 ttctaagtcg tccgtcttcg caactttaaa gtaaatcaca ttgttcgttg gtttgaagct
     2821 agaagctttt gtagcatgaa cttctactgc tggtatacca ttaatacgag ctctaacctg
     2881 ttcaatgaca gaatctaaat caccatcttt aatttcaaat ggcgctttaa ttgtcacatg
     2941 tggtttaatt cgtgaatact gtttatcata tcttttacgg taagaatcca ccgcttcttg
     3001 aaatgacttt gatggaatta atgctaatcc taaaatcatc tgaattcctc c
//
LOCUS       DAMQYT010000239         3000 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMQYT010000239 DAMQYT010000000
VERSION     DAMQYT010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.246"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..379
                     /gene="lpl4"
                     /locus_tag="PGA33_002515"
     CDS             <1..379
                     /gene="lpl4"
                     /locus_tag="PGA33_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl4"
                     /protein_id="HDG6474284.1"
                     /translation="ILLDKVEDKKLKQKIENFKFFSQYANLKELKNYSNGDVSINENV
                     PSYDVKYKMSNKDENVKQLRSRYNIPTDKSPVLKMHIDGNLKGSSVGDRKLEIDFSKR
                     ENSHLSVIDSLDYQPAKTNKDDE"
     gene            398..1168
                     /gene="lpl5"
                     /locus_tag="PGA33_002516"
     CDS             398..1168
                     /gene="lpl5"
                     /locus_tag="PGA33_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HDG6474285.1"
                     /translation="MKSIKRIGLCISLLILSIFVTSCDGDNKITGDSKETQIKKSFSK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKGNKSDELDDEGMVLNLDR
                     NTRTAKGYYFVKKFYEKDKLPDRKNYKVEMKNNKIILLDKVEDPNLKKRIENFKFFGQ
                     YANFKDLENYNNGDVSINWNVPSYDVEYKMSNKDENVKQLRSRYNIPTDKAPMLKMHI
                     DGDLKGSSVGYKRLEIDFSKEGRDISVIDYLSYKPAKK"
     gene            1200..1991
                     /gene="lpl6"
                     /locus_tag="PGA33_002517"
     CDS             1200..1991
                     /gene="lpl6"
                     /locus_tag="PGA33_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl6"
                     /protein_id="HDG6474286.1"
                     /translation="MEYLKRLALLISVIILTIFIMGCDSQSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLEDFYGKEGYRDGEFKKDDKGTWLIRSEIVKQPKGKVMKTRGMQL
                     YINRNTETAKGFFVLKEISENNNRVNKDKEEKYEVKMVGNKIIPTEQINDEKIKKEIE
                     NFKFFVQYGNFKNFEKYNNGEFSYNPEAPIYSAKYQLHNDDYNVRQLRKRYDISTKET
                     PKLLLKGGGDLKNSSVGQNDIEFTFVERKGENIYFNDSVEFIPSK"
     gene            2061..2837
                     /gene="lpl7"
                     /locus_tag="PGA33_002518"
     CDS             2061..2837
                     /gene="lpl7"
                     /locus_tag="PGA33_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl7"
                     /protein_id="HDG6474287.1"
                     /translation="MKCFQKLYIFILILIVLMAGCESNKITGDSKETQIKKSFAKTLD
                     VYPTKNLEDFYDKEGYRDGEFKKGDKGKWVIRSEMTTELKNENMVSKGMVIRLNRNSR
                     TCTGEYFVRIVKEDSEGKVYSDERKYPVKMENNKIITLKPIDDEKVKKEIEEFKFFVQ
                     YGNFKELENYKDGEVTYNPEAPIYSAQYQLKNSDYNVEQLRKRYNITTKKAPKLLLKG
                     SGNLKGSSVGYKNIEFTFVENKEENIYFTDSINFNPSEDK"
BASE COUNT         1287 a          301 c          557 g          855 t
ORIGIN      
        1 cattttatta gataaagtag aagataaaaa gcttaaacaa aaaatagaaa attttaaatt
       61 tttcagtcaa tatgcaaatc ttaaagaatt gaaaaactac agcaatggtg atgtctcaat
      121 taatgagaat gttccaagtt atgacgtgaa atataaaatg agtaataaag atgaaaatgt
      181 taagcaatta agaagtcgtt ataatattcc tactgataaa tcaccggtat taaaaatgca
      241 tattgatggt aatttgaaag gaagttccgt tggagatagg aagttagaaa ttgatttttc
      301 gaaacgtgaa aatagccatt tatcagtaat agattctttg gattaccagc cagcgaaaac
      361 taataaagat gatgaataat gaggatggtg tgtaacaatg aagtctataa aaaggattgg
      421 attgtgcata agtctgttga ttttaagcat ttttgtaaca tcttgcgatg gtgataataa
      481 gatcactgga gattcaaaag aaacacaaat caaaaagagc ttttcgaaaa cgttagatat
      541 gtatccaatt aagaatctcg aggacttata tgataaagaa ggataccgtg atggcgaatt
      601 taaaaagggc gacaaaggaa tgtggactat atatacagat tttgctaaag gcaataaatc
      661 agacgaattg gatgatgaag gtatggtttt aaatctggat agaaatactc gaacggctaa
      721 gggatattat tttgttaaga aattttatga aaaggataaa ttacctgata gaaaaaatta
      781 taaagttgaa atgaaaaata ataaaattat cttattagac aaggtagaag atccaaatct
      841 aaaaaagaga atagaaaact ttaaattttt cggacaatat gcaaatttta aggatttgga
      901 aaattacaac aatggcgacg tgtcaataaa ttggaatgtt ccaagttatg acgtggaata
      961 taaaatgagc aataaagatg aaaatgttaa acaattaaga agtcgttata acattcctac
     1021 tgataaagct ccaatgttaa aaatgcatat tgacggggac ttaaaaggta gttctgttgg
     1081 atataaaagg ttagaaatag atttttcaaa agaaggtagg gatatttcag tcattgatta
     1141 tttaagttat aagccagcga aaaaatagta ctgataatta aatattaggg tgtgaaggaa
     1201 tggaatatct aaaaaggctt gcattgttaa taagtgttat tattttgacc atttttataa
     1261 tgggttgtga tagtcaaagc gatactgcag aaaatccaaa agaaggttca aaagaagcac
     1321 aaattaaaaa gagtttttcg aaaacgttag atatgtatcc aattaagaat ctcgaggatt
     1381 tttatggcaa agaaggatat cgagatggcg aatttaaaaa agatgataaa ggtacttggc
     1441 taattagatc cgaaatagtt aaacagccaa agggcaaagt gatgaaaaca agaggtatgc
     1501 aattatatat taatagaaat accgaaacag ccaaaggttt ctttgttttg aaagaaataa
     1561 gtgaaaataa taatcgtgta aataaagata aggaggaaaa atacgaagtg aaaatggtag
     1621 gaaataaaat tattcctact gaacaaatta atgacgagaa aataaaaaaa gaaattgaaa
     1681 acttcaagtt ttttgtgcaa tatggaaact ttaaaaattt cgaaaaatac aacaatggtg
     1741 agttttcata taatcctgaa gcaccaattt attcggctaa atatcaatta cacaacgatg
     1801 attacaatgt aaggcaacta cgtaaaagat atgacatttc aacaaaagaa acaccgaagt
     1861 tacttttgaa aggtggagga gatttaaaaa attcctcagt tggtcaaaac gatattgaat
     1921 ttacttttgt tgaaagaaaa ggtgagaata tttattttaa cgatagtgtt gaattcatac
     1981 caagtaagta aagttaaatt aaaatgtgtg gttagatgaa atgatcaaat atcaaaaaat
     2041 gtgcaaagag atgtgacaag atgaagtgtt ttcagaaatt atacatattt atattaattt
     2101 taatcgtatt aatggcagga tgcgaaagta ataagatcac tggagattcg aaagaaacac
     2161 agatcaaaaa gagctttgcg aaaacattag atgtataccc tacgaaaaat ctagaagatt
     2221 tttatgacaa agaaggatat cgagatggcg aatttaaaaa gggtgacaaa gggaagtggg
     2281 ttattagatc tgaaatgaca acagaactga aaaatgaaaa tatggtatct aaaggtatgg
     2341 tcatacgttt aaatagaaat agtagaacat gcactggtga atattttgtc aggatagtta
     2401 aagaagacag tgagggcaag gtatatagtg atgaacgaaa atatccagtg aaaatggaaa
     2461 ataataaaat cattacatta aaaccaatcg atgatgaaaa agtaaaaaaa gaaattgaag
     2521 aatttaaatt cttcgtacaa tacgggaatt tcaaagaatt ggaaaactat aaagacggag
     2581 aagtgacata taacccagaa gcaccaatat actctgcaca atatcaattg aaaaatagtg
     2641 attataatgt agaacaacta cgtaagcgat ataatataac gacgaaaaaa gcgcctaaat
     2701 tattattgaa gggttcaggt aatttaaaag gctcatcagt tggatataaa aatattgaat
     2761 ttacctttgt tgaaaataag gaagaaaata tttacttcac agatagtatt aatttcaacc
     2821 caagtgagga taaataagta tatttagtat tacaaaaacg ttagaaaaat taataataaa
     2881 gttaaattaa caaactgtat taaattcata tagtcagttt tgtttgatga catgatagta
     2941 gaaaaattat tattttaata ggttgtatga actatacaaa tatttataaa aattaaaaca
//
LOCUS       DAMQYT010000240         2970 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMQYT010000240 DAMQYT010000000
VERSION     DAMQYT010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.247"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            41..391
                     /locus_tag="PGA33_002519"
     CDS             41..391
                     /locus_tag="PGA33_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HDG6474288.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     ILNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTDG"
     gene            384..947
                     /locus_tag="PGA33_002520"
     CDS             384..947
                     /locus_tag="PGA33_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDG6474289.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            961..1305
                     /gene="gpsB"
                     /locus_tag="PGA33_002521"
     CDS             961..1305
                     /gene="gpsB"
                     /locus_tag="PGA33_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDG6474290.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            1368..1747
                     /gene="rnpB"
                     /locus_tag="PGA33_002522"
     ncRNA           1368..1747
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PGA33_002522"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            1956..>2970
                     /locus_tag="PGA33_002523"
     CDS             1956..>2970
                     /locus_tag="PGA33_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDG6474291.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILT"
BASE COUNT         1096 a          412 c          581 g          881 t
ORIGIN      
        1 agtatattgt gataaattaa aaaatgtagg aggcgtcctc atgaatgatg tagtcgaatc
       61 actaatttat gaagttaaca acatgcaaca aaattttgaa aatgtgaaat cacaacaaca
      121 agatcatgat ttttaccaaa ctgtaaagcc atatactgaa catattgata gcatactcaa
      181 tgagatcaaa ttacatcgtg aatttattat agaagtacct tatatgaatt caaggaaatt
      241 tgagctactg attgctaaca ttgaacaact ttctgtcgaa tgtcatttta agcgaacaag
      301 tcgaaagtta tttatagaaa agcttaaaag tgttcaatat gatttacaaa atatattaga
      361 tggcgtaaca aaagagggta ctgatggtta aaacagttta tgtaacaggt tacaaatcat
      421 tcgaattaaa catttttaaa gatgacgcac ctgaagtaca ttatttaaaa caatttataa
      481 aacataaaat tgaacaactg ttggatgaag gattagaatg ggtgttaata caagggcaaa
      541 tgggtataga attatggact gcagaagttg ttattgaatt acaacgaaca tatgattcgt
      601 taaagtttgc tgtaattaca ccgttccaag gacatacaga aaagtggaat gaacataatc
      661 aaagtaagta tgccaacata attaagcatg cagattatgt tgatagtatt tttcatacgt
      721 cgtatcaagg tccttttcaa tttaaacaag cagatcaatt tatgcttgaa cattcggatc
      781 aaacattgct catttatgat gaggaacaag aggcgagtcc taagttcttc aagcagatgt
      841 tagttgattt tatggataaa acaaactata cttgtgatat tgtgacgttc gatgaattaa
      901 cagcattcat caatgactta cagtggtctg aagatcaaag tttctaatga ggtggaaaaa
      961 atgtcagatg tttcattgaa attatcagca aaagatattt atgaaaaaga ttttgaaaaa
     1021 acgatggctc gtggctatag aagagaagaa gtagatgcat ttttagatga cattattgct
     1081 gattatcaaa aaatggccga tatgaataat gaagttgtaa aattatcaga agagaatcat
     1141 aaacttaaaa aagaattaga agaattaaga ctacgtgtag caacatcaag acctcaggac
     1201 aataaaagtt tttcttcgaa taatacaaca acaaatacat cttcaaataa tgtagatatt
     1261 ttaaaacgta tttcaaactt agaaaaagct gtatttggta aataattact aaatacaaaa
     1321 gtttaactgt cttactaata atgactatgt tataatttta aaagtgatat tttgggtaat
     1381 cgctatatta tatagaggaa agtccatgct cacacagtct gagatgattg tagtgttcgt
     1441 gcttgatgaa acaataaatc aaggcattaa tttgacggca atgaaatatc ctaagtcttt
     1501 cgatatggat agagtaattt gaaagtgcca cagtgacgta gcttttatag aaatataaaa
     1561 ggtggaacgc ggtaaacccc tcgagtgagc aatccaaatt tggtaggagc acttgtttaa
     1621 cggaattcaa cgtataaacg agacacactt cgcgaaatga agtggtgtag acagatggtt
     1681 atcacctgag taccagtgtg actagtgcac gtgatgagta cgatggaaca gaacatggct
     1741 tatagaaata tcactactag tttagctctc ctagatgatg gagagctttt ttcatgaaaa
     1801 gaacacttaa aattaacgcc ttgtcttgat ataatgacac tgccttgttt taaaatagta
     1861 agcggatgcg ttaatgtatc agcgattaaa tttgttggaa atgtataaaa aacacaagct
     1921 aagaataaaa tacctgtata aaaggagaat catatatgtt tcaattactt gcagtttgtc
     1981 cgatgggatt agaagctgtt gttgctaggg aaattcaaga attaggctat gaaacaaatg
     2041 ttgaaaatgg tcgtatattt tttgaaggag acgcaagtgc aattgtaaag gcaaatttat
     2101 ggttgcgcac agcagaccga atcaaaattg ttgttggacg ttttaacgca acaacgtttg
     2161 acgaattatt cgaacaaacc aaagcgctcc cttgggaatc tataattgat aaagagggta
     2221 acttcccagt tcaaggtaga agcgttaaat caacactaca tagtgtgcca gattgtcagg
     2281 cgataactaa gaaagctatt gttgaacgat tacgtcgcgc atataacgaa aaaggttggt
     2341 taaatgaatc aggtgccaaa taccctgttg aagttgccat tttaaaagat aatgtattat
     2401 tgactatcga cacatcaggt tctggtttga acagacgtgg ttatagatta gcacaaggtg
     2461 aagcaccaat taaagaaacg ttggcagcaa gtttaatccg tcttgcaaac tggaaaggtg
     2521 atacaccttt aattgatcca ttttgcggtt cgggaacaat agctatagaa gcttgtttaa
     2581 ttgctcaaaa tatcgcacct ggttttaatc gcgagttcgt atcagagcaa tggaacatca
     2641 tgccagcaaa tatttatgat gattaccgtg atgaagcgga taagatggct gattatgata
     2701 aagaaatcga agtatatgct tctgatatcg atccagaaat ggtagagatt gctaagcgta
     2761 acgctgaaga agttggattg tctgatatta ttaaatttag tgtaaaagat gtcaatacat
     2821 taacaattga tacagaagaa ccggtggcgt taattggaaa tcctccatat ggtgaacgta
     2881 ttggtgatcg tgaagaagtt gaagaaatgt accgttatat tggtaaacta atgaaacaac
     2941 atccattttt atctacatac attttaacaa
//
LOCUS       DAMQYT010000241         2895 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMQYT010000241 DAMQYT010000000
VERSION     DAMQYT010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.248"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            196..1560
                     /gene="radA"
                     /locus_tag="PGA33_002524"
     CDS             196..1560
                     /gene="radA"
                     /locus_tag="PGA33_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDG6474292.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEVLSF
                     ALHS"
     gene            1585..2658
                     /locus_tag="PGA33_002525"
     CDS             1585..2658
                     /locus_tag="PGA33_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDG6474293.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
BASE COUNT         1042 a          386 c          555 g          912 t
ORIGIN      
        1 attttatctt tgaaagatac cgactatcaa catgatgtaa gtttatttta taatattcat
       61 aaaaaataaa tctggtaaaa cagtttgcgt tagtagtcat gttaaaatac aaacaaatgt
      121 aactcacatt taatttgtca taatgggaat gtgcgtttaa aatagatttg ttcaaaggaa
      181 agtggaggtg caattttggc caagaaaaaa gtgatttttg aatgtatggc ttgtggttat
      241 caatctccta aatggatggg gaaatgtcct aattgtggcg cttggaatca aatggaggaa
      301 attgttgaaa aagcagccaa tcctaaacat ggagttaaaa ccaaggaatt agcaggtaaa
      361 gtacaaaaat taaatagtat taaacatgaa acaacgccga gagtgttaac agattcagca
      421 gaattcaacc gtgtattagg tggaggtatt gtgagcggat cgttagtact tattggtggg
      481 gatccaggta ttggtaagtc aacgttactt ttacaaattt gtgcatcgtt atctcaaaag
      541 aaaaaagtac tatatattac tggagaagaa tcgcttagtc agactaaatt acgtgcagag
      601 cgattagatg aagattcaag tgaattgcaa gtattagctg aaacagatct tgaagttatt
      661 tatcaaacag taaaagaaga acaacctgat ttattagtag tggattcgat tcaaacaata
      721 tatcatcctg aaatcagctc tgcgccaggt tctgtttcac aagttcgtga aagtacacaa
      781 agtttaatga atattgctaa acaaatgaac attgcaactt ttatagtggg tcatgtaacg
      841 aaagaaggtc aaattgctgg cccaagattg ctagaacaca tggttgatac tgtgctttat
      901 tttgaaggcg atgaacacca cgcatatcga attttgcgag ctgttaaaaa ccgttttggt
      961 tcaacgaatg aaatgggaat cttcgaaatg aagcaaagtg gattaaaagg tgtaaataat
     1021 ccatctgaaa tgtttttaga agaacgttca acaaatgttc caggttcaac aattgttgca
     1081 accatggagg gaaccagacc acttttaata gaagttcaag cgctggtaac tccaacgact
     1141 tttaacaatc cgagacgaat ggcaacaggg attgatcata atcgattaag tttgttgatg
     1201 gctgttttgg aaaagaaaga aaattatcta ttacaacaac aagatgctta tatcaaagta
     1261 gctggcggtg taaagttaac ggagccagca gttgatttaa gtgtaattgt agcaactgca
     1321 tctagcttta aagataaagc tgtcgacgga ttagattgct atattggaga agttggttta
     1381 acgggtgagg tacgtcgtgt atctcggata gaacaacgcg tgcaagaggc tgcaaaacta
     1441 ggtttcaaac gtgtaattat tcctaaaaat aatataggcg gatggacata tcctgaaggt
     1501 atacaagtaa taggtgtaac tactgtacat gaagtattgt catttgctct tcattcataa
     1561 aacatcaaga aaggaggaca ttgtgtgaat atcgttaaac taatggttat tattatttac
     1621 ttaattattg ggagcgcatt aggaataatt attattcctg aaattgcaaa tgatcttgga
     1681 ttacaaaact ccagcttttt aaaaaatcac tatgtagatg gcattatcgg tagtattttt
     1741 atgttcttaa tttttggtgt atttattaga cgagttacta acgctataaa aggtttagaa
     1801 cattttatta tgcgtagaag tgctgttgaa atactattcg caacaatagg tttaataatc
     1861 ggattactta tttctgttat ggtgtcgttt atattagaat caattggcaa ctctattttt
     1921 aatcatttca ttcctgtcat aattacgata ttactatgtt atttcggttt ccaatttggc
     1981 cttaaaaaac gagatgaaat gttaatgttt ttacctgaga atatagcgcg ttccatgtca
     2041 caacatacta aaagtgctac gccaaaaatt atcgacacaa gcgcaattat tgatggtcgt
     2101 attttagaag tcattcgttg cggttttatc gatggcaata ttttaattcc acaaggtgtt
     2161 attaatgaat tacaaattgt tgcagattca aatgacagtg ttaaacgtga aaagggtaaa
     2221 agaggcttag atattttaaa tgaattgtat gatttagact atcctacaaa ggttatacat
     2281 ccaactaaaa cacatagtga tattgatacg atgttattaa aattagcaaa acaatatcat
     2341 gcaagtatta taacgacaga tttcaaccta aataaagttt gtcatgtaca tggtatcaaa
     2401 gcattaaatg ttaatgattt atcagaagca atcaaaccta atgtacatca aggtgatcaa
     2461 ctgcatattt tactgacaaa aatgggtaag gagcctggtc aggcagtagg atatctagat
     2521 gatggtacga tggtagttgt tgataatgct aaaaatctta ttggcagtca tgtcaattta
     2581 gaagtagtca gcttattgca aacatcttca ggaagaattg tttttgctaa aaaaatcgaa
     2641 gatacagtat cattataaaa actgtagttg acgaaaacaa aatagatatt aaaaggtata
     2701 tgaaatggcg aagtttgtaa tttgaagtat aactataaat aaatgcaaaa catattagtt
     2761 atcaaaaaat acaaatagtg ctaatatatc attgaaacaa gttcattaag tgttaaattt
     2821 tagatattaa taaagttatt cttatagcaa tcaagacata caaaattaga atcaatttta
     2881 acgtatttga atagt
//
LOCUS       DAMQYT010000242         2889 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMQYT010000242 DAMQYT010000000
VERSION     DAMQYT010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.249"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            14..955
                     /locus_tag="PGA33_002526"
     CDS             14..955
                     /locus_tag="PGA33_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDG6474294.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASLKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSDGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKIKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            967..2211
                     /gene="fabF"
                     /locus_tag="PGA33_002527"
     CDS             967..2211
                     /gene="fabF"
                     /locus_tag="PGA33_002527"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDG6474295.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVNS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(2266..2637)
                     /locus_tag="PGA33_002528"
     CDS             complement(2266..2637)
                     /locus_tag="PGA33_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDG6474296.1"
                     /translation="MLKKWLGMALITPILTFIIWLFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
BASE COUNT         1011 a          415 c          590 g          873 t
ORIGIN      
        1 aaggtgttca actatgaacg tgggtattaa aggttttggt gcatatgcgc cagaaaagat
       61 tattgacaat gcctattttg agcaattttt agatacatct gatgaatgga tttctaagat
      121 gactggaatt aaagaaagac attgggcaga tgatgatcaa gatacttcag atttagcata
      181 tgaagcaagt ttaaaagcaa tcgctgacgc tggtattcag cccgaagata tagatatgat
      241 aattgttgcc acagcaactg gagatatgcc atttccaact gtcgcaaata tgttgcaaga
      301 acgtttaggg acgggcaaag ttgcctctat ggatcaactt gcagcatgtt ctggatttat
      361 gtattcaatg attacagcta aacaatatgt tcaatctgga gattatcata acattttagt
      421 tgtcggtgca gataaattat ctaaaataac agatttaact gaccgttcta ctgcagttct
      481 atttggagat ggtgcaggtg cggttatcat cggtgaagtt tcagatggca gaggtattat
      541 aagttatgaa atgggttctg atggcacagg tggtaaacat ttatatttag ataaagatac
      601 tggtaaactg aaaatgaatg gtcgagaagt atttaaattt gctgttagaa ttatgggtga
      661 tgcatcaaca cgtgtagttg aaaaagcgaa tttaacatca gatgatatag atttatttat
      721 tcctcatcaa gctaatatta gaattatgga atcagctaga gaacgcttag gtatttcaaa
      781 agacaaaatg agtgtttctg taaataaata tggaaatact tcagctgcgt caataccttt
      841 aagtatcgat caagaattaa aaaatggtaa aatcaaagat gatgatacaa ttgttcttgt
      901 cggattcggt ggcggcctaa cttggggcgc aatgacaata aaatggggaa aataggagga
      961 taacgaatga gtcaaaataa aagagtagtt attacaggta tgggagccct ttctccaatc
     1021 ggtaatgatg tcaaaacaac atgggagaat gctctaaaag gcgtaaatgg tatcgataaa
     1081 attacacgta tcgatactga accttatagc gttcacttag caggagaact taaaaacttt
     1141 aatattgaag atcatatcga caaaaaagaa gcgcgtcgta tggatagatt tactcaatat
     1201 gcaattgtag cagctagaga ggctgttaaa gatgcgcaat tagatatcaa tgaaaatact
     1261 gcagatcgaa tcggtgtatg gattggttct ggtatcggtg gtatggaaac atttgaaatt
     1321 gcacataaac aattaatgga taaaggccca agacgtgtga gtccattttt cgtaccaatg
     1381 ttaattcctg atatggcaac tgggcaagta tcaattgact taggtgcaaa aggaccaaat
     1441 ggtgcaacag ttacagcatg tgcaacaggt acaaattcaa tcggagaagc atttaaaatt
     1501 gtgcaacgcg gtgatgcaga tgcaatgatt actggtggta cagaagcacc aattactcat
     1561 atggcaattg ctggtttcag tgcaagtcga gcgctttcta caaatgatga cattgaaaca
     1621 gcatgtcgtc cattccaaga aggtagagat ggttttgtta tgggtgaagg tgctggtatt
     1681 ttagtaattg aatctttaga atcagcacaa gctcgaggtg ccaatattta tgctgagata
     1741 gttggctatg gtactacagg tgatgcttat catattacag cgccagctcc agaaggtgaa
     1801 ggtggttcta gagcaatgca agcagctatg gatgatgctg gtattgaacc taaagatgta
     1861 caatacttaa atgcccatgg tacaagtact cctgttggtg acttaaatga agttaaagct
     1921 attaaaaata catttggtga agcagctaaa cacttaaaag ttaactcaac aaaatcaatg
     1981 actggtcact tacttggtgc aacaggtgga attgaagcaa tcttctcagc gctttcaatt
     2041 aaagactcta aagtcgcacc gacaattcat gcggtaacac cagatccaga atgtgatttg
     2101 gatattgttc caaatgaagc gcaagacctt gatattactt atgcaatgag taatagctta
     2161 ggattcggtg gacataacgc agtattagta ttcaagaaat ttgaagcata actataaaaa
     2221 tcttcagtaa cgttgtttta gttactgaag attttttcag tttctttata ctaagatgag
     2281 cgacagcaca atcgtcataa taaaatataa aatatttatt aataataagg ggattatcca
     2341 tgtagaaaca aagtaatgct ctttttttac ctcttgtggg ttgaaaaatg gatcatcaga
     2401 gatagacttc ttctttttcg aagatgacat ttgatacttt aatcttctaa aaccataact
     2461 tgtcgcatca aaaatgcctt cttgtacaag taaaatcaaa aatatgctaa taaaaataat
     2521 taatgaaaca taaaacaata tatttaaata tgtaatgata gtatggctat taaaaagcca
     2581 tataataaac gttaatattg gcgttattag tgccattcca agccattttt tcaacatttg
     2641 atcactccca cttatagaaa actcttacgc atagtttaca ttaaaatcag acattgagga
     2701 atgatttttt aatttcttca gctttattga aattctaaaa tcaatcattc ttcattagtt
     2761 taaagcaaaa aaatattgat atatagtaaa tattgtatat ataatattag ttaagatttc
     2821 agaaaatttt gaagggaatg gaaatttaga aatcggaatt tgttagagga ggggattaga
     2881 tggggaaat
//
LOCUS       DAMQYT010000243         2824 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMQYT010000243 DAMQYT010000000
VERSION     DAMQYT010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.250"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..387
                     /locus_tag="PGA33_002529"
     CDS             <1..387
                     /locus_tag="PGA33_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDG6474297.1"
                     /translation="SEIVKSILHNNLRFISPPKGGNDKNRKRWPLYRPWALFIKDTEK
                     LNLTTRPTLKSIEDNLDWLCKQVATTLDTVLTAESMAQSEGLLTDTDFLDKILAHSQF
                     NDEHTNRINHYLEALKQKKHLSKDKC"
     gene            448..1503
                     /locus_tag="PGA33_002530"
     CDS             448..1503
                     /locus_tag="PGA33_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDG6474298.1"
                     /translation="MKIISKYILTRFSKNGKHFDIPKEQRRVIPKFKNRRKMIVICFY
                     SLLAILLILLLASFIKATRANNDSKEAVNKTNMIQKKYEDNANTVQYSPKLKLYADKF
                     IDTYMNISKDSKELESREKELLKYFPSDYKKPDEKISDTERKLNSKEFYNIKRKDKQT
                     IIQYIVNYDVNITEKKEVKVKKKKKSEKDKDEYETKTEEKQRKVNQNILINIPIKSEN
                     NKYVVVEYPYFTPIPDSQLNQAKMVEDNLKDNKREDNPKAKAFIEDFFNKYASSKSDD
                     MAYLMDNPEGLEGTREVSQIREIRLYPKGDDYVAKVEILMKDKDSPLENLEHYTLDIT
                     KKDGKYYVKNMTNSIGG"
     gene            1508..1768
                     /locus_tag="PGA33_002531"
     CDS             1508..1768
                     /locus_tag="PGA33_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474299.1"
                     /translation="MSGLFNFFVLGADRPSLGGVGDWISNEVGTGIGLVVLVVGIVKW
                     ASGKYGHMVILFVVGGFLFLVSKGPEQVFNAIAGVWKMIFGG"
     gene            1780..2163
                     /locus_tag="PGA33_002532"
     CDS             1780..2163
                     /locus_tag="PGA33_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDG6474300.1"
                     /translation="MDKKAYNLKRTFEQPIVMYEFTDKFRINKGFRLDFWATFLIVWF
                     ILFLLFWYLLQPVIMSIGGLMFIYFTVAPYYITKYIVKLKQDGKKLFFFLWDFVIFVF
                     NVQLRKVKLSYDEEVEYHDKKITFK"
     gene            2198..>2824
                     /locus_tag="PGA33_002533"
     CDS             2198..>2824
                     /locus_tag="PGA33_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDG6474301.1"
                     /translation="MNLKAPYAGLRDNLLLTKTGEVWAYYRVRSESISTANYDAKEES
                     KKRMRYFLESIKGYQDFHFEMYDHDLDLRNKFKEISKDFDDEAFNVAEYYSKETIDVL
                     EQELQMITHNSFVMGVKIRELADDAVTLKEILKSTLSDTAERIVSNFGFDVNLDDKIL
                     DKYKTFERDLADSFLGIGGERLTENELAYIIRKPFISNATHDVQEESSR"
BASE COUNT         1040 a          284 c          532 g          968 t
ORIGIN      
        1 agcgaaattg taaagtctat attgcataat aatttgcgtt ttatctctcc tcctaaaggt
       61 ggaaatgata aaaatcgtaa gcggtggcca ttatatagac cttgggcgtt atttattaaa
      121 gatactgaaa aattgaattt aactactaga ccaacattaa agtccattga ggataatctt
      181 gattggttat gtaaacaagt tgctactact ttagatacag tgttaactgc cgaaagtatg
      241 gcgcaatctg aggggttgct tacagatact gattttttag ataaaatttt agcgcattca
      301 caatttaatg atgagcatac gaatagaatt aatcattatt tggaggcttt aaaacaaaaa
      361 aagcacttat caaaagataa gtgttaaaaa aataactggg atgtgatttt tattaaaaat
      421 ttcgtaaagg attttagaaa atcgtttatg aaaataataa gcaagtacat tttaacacga
      481 ttttctaaaa acggaaagca ttttgatatt cctaaagaac aacgccgagt aattccaaaa
      541 tttaaaaatc gtcgtaagat gattgtaatt tgtttttact cattattagc tattttgtta
      601 attcttttat tagcttcttt tattaaagct acaagagcaa ataatgacag taaagaagca
      661 gttaataaaa cgaatatgat tcaaaaaaag tatgaagata atgcgaatac agttcagtat
      721 agtcctaaat taaagttata tgctgataag tttattgata cgtatatgaa tatctcgaaa
      781 gattcaaaag aattggaatc tagagaaaaa gaattgttga aatattttcc atcagattat
      841 aaaaaacctg atgaaaaaat ttcagataca gaacgaaaat tgaatagtaa agaattttat
      901 aatattaaac gtaaagataa acaaacaatt atacagtata ttgttaatta tgatgtgaat
      961 attactgaaa agaaagaagt taaagttaag aagaaaaaga aaagtgaaaa agataaagat
     1021 gagtatgaaa caaaaacaga agaaaagcaa cgtaaagtaa atcaaaacat tttaataaat
     1081 atacctatta agagtgaaaa taataagtat gttgttgttg aatatcctta ttttacgcca
     1141 atccctgata gtcaattgaa tcaagctaaa atggttgagg ataatttgaa agacaataaa
     1201 cgggaagaca atccgaaagc aaaagctttt attgaagatt tctttaataa atatgcttct
     1261 agtaagtctg atgatatggc ctatttaatg gataaccctg agggcttaga aggaactaga
     1321 gaagtttctc agataagaga aattaggtta tatccaaaag gcgatgatta tgttgctaaa
     1381 gttgaaattt tgatgaaaga taaagattct cctttggaga atttggagca ttacacatta
     1441 gatataacaa aaaaagatgg taaatattac gttaaaaata tgactaattc tattggaggt
     1501 taatgttatg agtggtttat ttaatttttt tgttttaggt gctgaccgtc catcacttgg
     1561 tggagttggt gactggataa gtaatgaagt aggtacgggt attggtttag tagtattggt
     1621 tgttggtatt gtaaaatggg ctagtggtaa atatgggcat atggttatat tatttgttgt
     1681 tggtggcttt ttattcttag tttctaaagg tcctgaacag gtatttaatg cgattgcagg
     1741 tgtttggaaa atgatttttg gcggttgagg tgattccatt tggataaaaa agcttataat
     1801 ttaaagcgaa cttttgaaca accgatagtt atgtatgaat ttacagataa gtttcgtatt
     1861 aataagggtt ttcgtttaga tttttgggct acatttttga ttgtgtggtt tatattattt
     1921 ttgttatttt ggtatttatt acagcctgta attatgtcga ttggtggact tatgtttatt
     1981 tactttacgg ttgcacctta ttatataacg aaatatattg ttaagttaaa acaagatgga
     2041 aagaaattat ttttcttctt atgggatttt gtaatatttg tttttaatgt gcagttaaga
     2101 aaagttaaat taagttatga tgaagaagta gaatatcatg ataaaaaaat aacatttaaa
     2161 tagcccctat tggggttatt tttattggag ggatgacatg aatttaaaag caccgtatgc
     2221 aggtttaaga gataatttgt tattaacgaa aactggcgaa gtttgggcgt attatcgtgt
     2281 tcgttctgag tctatatcaa cagcaaatta tgatgcaaaa gaagaatcaa aaaagcgtat
     2341 gcgttatttt ttagagtcga ttaaaggtta tcaagatttt cattttgaaa tgtatgacca
     2401 cgatttagat ttgcgaaata aatttaaaga aattagtaaa gattttgatg atgaagcttt
     2461 taatgtagct gaatattatt caaaagaaac tattgacgta ttagaacaag aattacaaat
     2521 gattacgcat aattcttttg ttatgggtgt taaaatacgt gaattagctg acgatgcagt
     2581 tacacttaaa gagattttaa aaagtacatt atcagatacg gctgagcgta ttgtttcgaa
     2641 ttttggtttt gatgtgaatt tagacgataa aattttagat aagtataaaa catttgaacg
     2701 agatttggct gatagttttc taggtattgg aggcgagcgt ttaactgaga atgaattggc
     2761 ttatattatt agaaagcctt ttatttcaaa tgcaacacat gacgtacagg aagaatcttc
     2821 aaga
//
LOCUS       DAMQYT010000244         2795 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMQYT010000244 DAMQYT010000000
VERSION     DAMQYT010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.251"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            134..598
                     /locus_tag="PGA33_002534"
     CDS             134..598
                     /locus_tag="PGA33_002534"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDG6474302.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(720..1721)
                     /locus_tag="PGA33_002535"
     CDS             complement(720..1721)
                     /locus_tag="PGA33_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HDG6474303.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            1833..1952
                     /locus_tag="PGA33_002536"
     CDS             1833..1952
                     /locus_tag="PGA33_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474304.1"
                     /translation="MMLISFIYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            1955..2782
                     /locus_tag="PGA33_002537"
     CDS             1955..2782
                     /locus_tag="PGA33_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDG6474305.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
BASE COUNT          970 a          394 c          499 g          932 t
ORIGIN      
        1 tgagggatta aaacaatatg gcattgatat tgcagaaaga attgaagtta tcgtaccaga
       61 aacggtacat aatcatgatt atatggaaac gaaaaaaata aaaatgggtc atttaatata
      121 ggaggatttt aacatgaatt ttgaaggtaa attaattgga aaagatttga aagttgcaat
      181 cgtagttagt cgatttaatg attttatcac tggaagatta cttgaaggtg caaaagatac
      241 tttgattcga catgatgtta atgaagacaa tattgatgta gcatttgtgc caggtgcgtt
      301 tgaaattcct ttagtagcta aaaaattagc ctcatcagga aattatgatg cagtaattac
      361 attaggatgc gtaattcgcg gtgctacgtc tcattatgat tatgtttgta atgaagtcgc
      421 gaaaggtgtt tctaaagtga atgatcaaac taatgtacca gtcatatttg gtattttaac
      481 gactgaaagt attgaacaag ctgtggaaag agcaggtacg aaagctggca ataaaggtgc
      541 tgaagcagca gtaagtgcaa ttgaaatggc taatttatta aaatctataa aagcatagag
      601 cactgttgtg agttcataat caaagattta taaatgtttc agaactgcca atataacatt
      661 taggagccta gaacattgat tattgttcca gactcctaaa ttattgttta tgtcttgctc
      721 tatttacggc ataatttttt aattgtactt aaacctaaca taactgtagc tcctaaagct
      781 gcaattatgc caacacgttt taagccagca ggtttcacaa attcttttac agcaagagat
      841 aggttttgtg ggcgttctgc taatcttctc ataaaatacg caaaccaatc atcgccataa
      901 ggtacataaa tagtgaaatt atagccttca tttgcgattt cttctgctaa ctctgatcta
      961 aaaccataga gcatttggaa ttccatacga tctttttcaa tgtgattttc tttcataaat
     1021 tgttttacat gattaatgat gcgatggtca tgtgttgcaa ttgaagtgaa attgcgtgcg
     1081 tttaacaaac gttgttcaat tattttgatg taatttgcat ctacgtcttc cttagattga
     1141 aatgcaattg attcgttttc tttatatgca cctttaacca aacgtaatcg taaatcttgg
     1201 tacttatcaa ctaattcgtg gctatcgtat aaatatgctt gaattacagt accaacattt
     1261 ctaaattcgc cttttaagcg atctaaaact tgaacaattt gttgcaggct agcatatttt
     1321 tcagtatcaa tatttatatg catattgttg taagtatttg ctttaagtaa aatctctctt
     1381 aaattttggt aagctaattc taagtcgaat tctgcaccta actgactcaa tttaacagac
     1441 atatgtgcct ttacgccatg ttgatgaagc gcgtccataa ttgttaaaat ttgttcttta
     1501 gcatgattac tttcttcaac tgtaccgaca aattccccta aattgtctac cgtaacagcg
     1561 atattcttgt catttaagta ttcgattgta ttaattaact ctggaattgt atttccggca
     1621 acgactttat tggcgcccaa acgtgggccc actttttttg ctgcgttgtt taaaaaacta
     1681 ttattagata atccgataaa aaaattcttt aatagtgcca ttgctactcc tccaaaccct
     1741 tgtgtttaca ataagtttag catgaaaaaa atattcgaga aagcgttttc atcaaaaaca
     1801 atcaaaaata attatgtata attacactat tattgatgtt gatttcattt atatataata
     1861 atatggacaa caataatact ataaatagaa atctacatag tgaatttgct ataattatgt
     1921 tattgaagat taatgaaaaa ggggcagtat aataatgtgg aagtgggaag ctgaaaatga
     1981 tgcgaaaggc gtcgttgtta ttgctcataa tatattagaa catacgggaa gatatgcata
     2041 tgttattacg atgttaagaa gaaatggcta tcatgtgatt atgggagatt tgcctggtca
     2101 aggtcaaact tcaagagcta ataaaggaca aattgaaaac tttcaaacgt atcacgaaag
     2161 tttgctagac tggttaaaga tagctaatga atataaaatt cctacttatg tattaggggt
     2221 aggacttggc ggtttgattt tattaaattt acttgaaaaa gtagaattgc caatcgaggg
     2281 gatgatgtta atttctccta tgctagaact tcaaaagaat ggaaaaaatc gtaaagataa
     2341 acttgtttca aatataggta aaatttcaaa agacacacgt tttaatgttg gtgtagaacc
     2401 aaaagattta acacgtaatt tagaaattgt tgaagaaaca gttaacgatg gtttaatgtt
     2461 aaaaaaagca acgtatcatt ggtataatac aattaatgag acgatgaaag atacgatggc
     2521 tcatattcat gacattcaac caatgccaac gctattaatg tatggaacga aggatttaat
     2581 tgttgatact agggcaattg atgagtttaa agaaaaatat caaacacctg aattatattt
     2641 taaagcttgg caaggttttt accacgaggt tcacaatgag ccagaacgtg atgaagtaat
     2701 gcgttatatt ctgactttct taaataacag cgtcaataca atgggcttta ttgttgaaga
     2761 tgacgaaatt gtagaaattt aatattctaa aaagg
//
LOCUS       DAMQYT010000245         2764 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMQYT010000245 DAMQYT010000000
VERSION     DAMQYT010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.252"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..1178
                     /locus_tag="PGA33_002538"
     CDS             <1..1178
                     /locus_tag="PGA33_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDG6474306.1"
                     /translation="LASPEFDDLKRTDASQVDGQDDTSEDDDNESEKADSKSADSESK
                     GGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGDAVGQS
                     QAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIEREQRDA
                     RFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKMAETKK
                     GVVLFHETLKALNIKHEILSFSEDAFDSDEHAQPNIINEIINYDYSTFEKDGPRIMAL
                     EPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEAVEMSR
                     KFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLLLKSL"
     gene            1403..2746
                     /gene="brnQ"
                     /locus_tag="PGA33_002539"
     CDS             1403..2746
                     /gene="brnQ"
                     /locus_tag="PGA33_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDG6474307.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
BASE COUNT          949 a          397 c          487 g          931 t
ORIGIN      
        1 cattagctag tccagagttt gatgatttaa agagaactga tgcaagtcaa gtcgatgggc
       61 aagatgatac ttcagaagat gatgataacg aaagtgaaaa agctgactct aaatctgcag
      121 atagtgagtc aaaaggtggc gcctatttag aaatggagct tcacgaagga caaaatagtg
      181 aaacattagg taatgatgaa gcacgtgaag gcgatgctac agatgatatg actgatatga
      241 tgaccaaaaa gggcaaagga tcaaacgata cgctaaatcg tgaagaaggc gatgctgttg
      301 gtcaaagcca agcatttcaa cttgatggtg tgaacaaaaa tgtagaaata aaatggcaaa
      361 ttcctgaaat tgagccacaa tatgttttag aatatcaaga atcgaaacaa gacgtacaat
      421 atgaaatcaa agacttaatt caaattataa agaaaacgat tgaacgagaa caacgagatg
      481 cacgttttaa tttaacaaaa gggcgattac aaaaggattt aattaattgg tttatcgacg
      541 atcaatataa attgttttac aaaaaacaag atttaagtaa atcatttgat gcgacattta
      601 cattgttaat tgatgcgtca gcaagtatgc atgataaaat ggctgaaacg aagaaaggtg
      661 tagtgttatt ccacgagacg ttaaaagcat tgaacattaa acatgaaatt ttatcattca
      721 gtgaggatgc ctttgattca gacgagcatg cacaaccaaa tatcattaat gaaattatta
      781 actatgatta ctcaaccttt gaaaaagatg gcccacgtat tatggcactt gaacctcaag
      841 atgataatcg tgatggtgtt gctattagag ttgcaagtga acgattaatg cgtcgaaatc
      901 aacatcaacg atttttaatc gtattttcag acggcgaacc gtctgcattt aattatagtc
      961 aagatggtat tattgatacg tatgaagctg tagaaatgtc acgtaaattt ggtattgaag
     1021 tatttaatgt atttttgagt caagatccaa taactgaaga tgttgaacaa acaattcata
     1081 atatttatgg tcaatatgct attttcgttg aaggtgttgc gcatttacct ggacatctct
     1141 ctccattatt aaaaaaatta ctacttaaat ctttataaat aaatttaaaa tatgagttta
     1201 tatgaaattg tgaataaaag tcatcagtta ctatttaaaa agtggatttg aagaatttta
     1261 ttttctgaca taatttgatt taaacaacac atgtgttttg tttctgctat gtacaacata
     1321 tttcatatag actctttatt attttttgtg ctaaaatatt tacttgtaaa ttatttcgtg
     1381 ttttaaaaaa aggagtaact ctatgaataa aaatacatgg gtcattgggt ttacgctctt
     1441 cgctatgttt tttggcgcag gcaatctaat ttttcctcct aatctcggat tggatagtgg
     1501 tcaatttttc tggcccgcca ttttagcgtt tgttctaact gggattggtt taccattatt
     1561 aggtgtgatt gtaggtgcac ttgataaaga aggatatatt ggcgcattaa ataaaatttc
     1621 acctaaattt tcaatattgt tcttaatcat catttatttg actataggac cactttttgc
     1681 aatacctaga actgcatcta catcttttga aatgacaatt acaccaatta tacatagcaa
     1741 tagtagtatc gctttattta tatttacgat tatctacttc atagtcgttt tgtatatttg
     1801 tttaaatcca tctaagttaa tcgatcgtat tggttcatta ttaacaccat tattattgat
     1861 tactatttta gcgatgatta ttaaaggata cttagacttt agcggtaata gtgctggaaa
     1921 gggcaatgaa gcactatatc attctaattt ttcaagtttt gctgaaggct ttacacaagg
     1981 ctatttaaca atggatgcca ttgcagcaat tgctttttca atgattgttg ttaatgcagt
     2041 aaaactaaca ggcattacta aaacaaatca aatattcaaa caaactttga ctgctggttt
     2101 aattgcagcc gtagctttaa ttttcatata tatttcatta ggttatattg gtaatcatat
     2161 gccagtaagt gacatgacgt tagatcaatt gaaatccaaa gatcgaaaca ttgggacata
     2221 tttattaacg acaatggctt caacaggatt tggttcattc ggaaaatatt tattgggcat
     2281 cattgtggcg ctggcatgtc taactacagc atgcgggctt attgttgcag tttctgaata
     2341 tttccataga atcgtaccta aagtatcata caaagcattt gtattagttt tcattttaat
     2401 gagttttatt attgctaacc aaggtttaaa tgctgttatc tcaatgtcaa ttccggtatt
     2461 aagcattgta tacccagtag caataactgt tgtattatta attttaattg ccaaattcat
     2521 accgacaaaa cgcatttcac aacaaattcc agttattatc gtatttatat tgtcgatttt
     2581 cagtgttatt agtaagttag gttggctgaa aattaacttt atagaatcat tgcctctaag
     2641 agcgtattct ttagagtggt tcccagtagc aattattgca acgatattag gctatctagt
     2701 cggcatattt gtaaaacaag atccaattaa atatcaacag gaataacgaa taatataaaa
     2761 gagg
//
LOCUS       DAMQYT010000246         2762 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMQYT010000246 DAMQYT010000000
VERSION     DAMQYT010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.253"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..2262
                     /gene="recJ"
                     /locus_tag="PGA33_002540"
     CDS             <1..2262
                     /gene="recJ"
                     /locus_tag="PGA33_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDG6474308.1"
                     /translation="KYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQAIES
                     IISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTATTILVITLQLL
                     GAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLGVDVI
                     VTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFKALVA
                     IGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFIIGPR
                     LNAVGRLDDASLACELLMTDVEEEAAFLAEQVEHFNRERKDIVATITEEAMAMAETKV
                     KKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSIDQVSM
                     FEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVKQVDV
                     LLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTLGESN
                     IAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILDYRSK
                     RKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDLSNSL
                     QQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQHLSVK
                     PDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQLLYQD
                     FSEIKNWIKSQLS"
     gene            2284..>2762
                     /locus_tag="PGA33_002541"
     CDS             2284..>2762
                     /locus_tag="PGA33_002541"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDG6474309.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKI"
BASE COUNT         1039 a          379 c          526 g          818 t
ORIGIN      
        1 aagtataagt ggaaattaac taaaccagct gaatacataa gtgatgaatt aacatcaaaa
       61 ttaaagttaa cgccaatcgt aaaaaaaata ttagaaagta aatcgattat tgatgaacaa
      121 gcaatagaat ccatcatttc agatacagat atcaatcatg atgcactgca attgagcgat
      181 atgactaaga ctattgaaag aattaaaaga gcgatagcga atgatgagaa aatcctagtt
      241 tatggtgatt acgatgccga cggtgttacc gcaactacaa ttttagtgat aacattacaa
      301 cttttaggtg cacaagtagg atggcatatt cctaaccgct ttaccgaagg atatggacca
      361 aacgaactag catttagaaa tgctcatgat gaaggcataa cattaatcat aactgttgat
      421 aatggcatac aaggtcataa tgaaattaaa atggttcaag atttaggagt tgatgtcatt
      481 gtaactgatc atcatgaaat tggtagtaca ttaccagaag catatgcaat cgtacatcca
      541 atgcatccat cgtttaatta tccgttccaa caattgtgtg gtgcaggtgt tgcatacaaa
      601 ttagcacaag cattgattga aaatgtacct gattatttta aagcattagt tgcgataggt
      661 accattgcgg atttagtatc cttaactgat gaaaatcgat cattagtgaa acaaggatta
      721 aaagtcttaa atgatcaatg tccgacctct gttaaagcat tgctaaaaga agcgggatac
      781 aatgacaata ttgatgaaga aacaattggt tttattattg gacctagact taatgctgta
      841 ggtcgtttag atgacgcatc attagcttgc gaattgttga tgactgacgt cgaagaagaa
      901 gctgcatttt tagcagaaca agttgaacat tttaaccgtg aaagaaaaga tattgtagca
      961 actatcacag aagaagcaat ggctatggca gaaacgaaag ttaaaaaggg agatttattt
     1021 ttacttttag caaaagaaaa ttggcatgaa ggtgtcttag gtattgttgc ttcgaaaatt
     1081 gtcgaaacat ttgctttgcc aacattaatt ttaaatattg atagggagca aaatcatgca
     1141 aaaggctccg ccagatcaat tgatcaagtg tcgatgtttg aaatactatc agcacatcaa
     1201 gagttaatcg ctaaatttgg cggccatcat atggctgcag gtatgacgat ggatattgaa
     1261 aatatagaat cgttagctga aggtttaaat aaatggatga aagaattatc taaaacaact
     1321 tcgttagatc ccgtaaagca agtagatgta ttattaacag aaaatgatat aacgattaaa
     1381 aatattcgtg atatgaatcg tttaagaccc ttcggaacag atttttctag acctattttt
     1441 gaaatggatg atttatcagt ttcttcagtt aaggcgatag gtcaacaaaa aaatcatcta
     1501 aaattgacac ttggtgaaag taatatcgcc gcgctatttt ggcaaaatgg acacttagaa
     1561 cctgagttac aagatgaaca gccaattaat atattaggat ctgttcaaat caacgaatgg
     1621 aatggtaatc aatcaccgca aataattatt caagatattg cgatgaatga acagcaaata
     1681 ttagattata gaagtaagcg aaaaagttta ccttttacag aaaatgatga aaatattgtc
     1741 gtgcttattc atcctaaaag tgataaagta aatgcgaatg aatattatta tggtgaagaa
     1801 attaaacaac aaactgataa agtagtatta agagatttac caacgtcaat ggaagacttg
     1861 tctaattcct tgcaacaact gcaattttct caactttata tagttttgca acataatcat
     1921 tcgatttact tcgatggtat acctaatatg gatattttta aaaagtgtta taaagcatta
     1981 ataactaaac aagaaacaaa tatccagaaa gagggtatgt tattgtgtca acatttaagt
     2041 gtgaaaccag atacacttaa attcatgttg aaagttttct tagacttaaa atttgtaaca
     2101 caagaagatg gtttaattcg aatcaatcaa caacctgata aaagatcgat tgattccagc
     2161 aaagtatatc aattaagaca acaacgtatg gatgttgaaa agcaattatt atatcaagat
     2221 ttttcagaaa taaaaaattg gataaagtca caattgtcgt gagcaattta ggaggaaata
     2281 ttaatggatt taaagcaata cgtatcagaa gttcaagatt ggccgaaacc aggtgttagt
     2341 ttcaaggata ttactacaat tatggataat ggtgaagcat atggctatgc aacagataaa
     2401 attgtagaat acgcaaaaga cagagatgtt gatatcgttg taggacctga agcgcgtggc
     2461 tttatcattg gctgtcctgt agcttattca atggggattg gctttgcacc tgttagaaaa
     2521 gaagggaaat tacctcgtga agtcattcgt tatgagtatg acctagaata tggtacaaat
     2581 gttttaacaa tgcacaaaga tgcaattaaa ccaggtcaac gtgtgttaat tacagatgat
     2641 ttattagcta ctggtggtac gattgaagca gcaataaaat tagttgaaaa attaggcggt
     2701 atcgtagtag gtattgcatt tataattgaa ttgaaatatt taaatggtat tgaaaaaatt
     2761 aa
//
LOCUS       DAMQYT010000247         2737 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMQYT010000247 DAMQYT010000000
VERSION     DAMQYT010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.254"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            732..1106
                     /locus_tag="PGA33_002542"
     CDS             732..1106
                     /locus_tag="PGA33_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HDG6474310.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
     gene            complement(1357..2145)
                     /locus_tag="PGA33_002543"
     CDS             complement(1357..2145)
                     /locus_tag="PGA33_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDG6474311.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLKFLKSVS"
     gene            complement(2314..>2737)
                     /locus_tag="PGA33_002544"
     CDS             complement(2314..>2737)
                     /locus_tag="PGA33_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474312.1"
                     /translation="TGMSLFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTIIAII
                     SLLLIILQAFNITWGVIPITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTV
                     DAMIHNPQSAQIFSLIIWILLVVLVIYFTIRLSSRTRL"
BASE COUNT         1028 a          404 c          344 g          961 t
ORIGIN      
        1 tattcgtgct aatatttcat agttggttat tcaattaatt aaaaataagt caaaatgcac
       61 aactttttat aattcattga gtcgagtttg aaaaataaaa gtgctttaat gcatgatcaa
      121 ttatcgtact ttctattatt tgttacccgt tatcaatcgg aataacgtat agacacttta
      181 acgtgctata gattggtttt aatcactaaa ttaatgtgtt tttcttatca ttaaaactgc
      241 actgagaatt actaaattaa aaaaattata aaaatttttc atttttagtg ataaaattct
      301 gaaaaatggg tataaatagt agaagaagtt aacttggaag agttaagcta taacaaagaa
      361 tctctttaga cacacattga atatcgaaac atttaattgc gctaaatcgt ttcattaaat
      421 aaattacctt gtattgtcga ttaaattaag gtaaattata aaaaatgctg atatttttga
      481 ctaaaccaaa tgctaaccca gaaatacaat cactgtgtct aatgaataat ttgttttata
      541 aacacttttt tgtttacttc tcatttttaa ttagttataa ttaactaaat aatagagcat
      601 taaatatatt taataaaact tatttaatgc aaaattatga ctaacatatc tataataaat
      661 aaagattaga tatcaatata ttatcgggca aatgtatcga gcaagatgca tcaaataggg
      721 aggttttaaa catggcaatt acaaaaatca atgattgctt tgagttgtta tcaatggtca
      781 cttatgctga caaattaaaa agtttaatta aaaaggaatt ttcaattagc tttgaagaat
      841 tcgctgtatt gacatacatc agcgaaaaca aagagaaaga atactatctt aaagatatta
      901 ttaatcattt aaactacaaa caaccacaag ttgttaaagc agttaaaatt ttatctcaag
      961 aagattactt cgataaaaaa cgtaatgagc atgatgaaag aactgtatta attcttgtta
     1021 atgcacaaca acgtaaaaaa atcgaatcat tattgagtcg agtaaataaa cgaatcactg
     1081 aagcaaacaa cgaaattgaa ctataatttt gtttagcgca atttggtgaa gtttgataga
     1141 tgatacattc tattaaactt ccttttttta tgctcttttt acctaattgt taagaggttt
     1201 tgcactaatg gcacttaata taaggaagat agccgcataa cgagcagtat atatcaaata
     1261 catttcaaaa aaagctgatt tctatcaaat aatgaatagt aatcagcttt ttcacattgc
     1321 ctaaggactt tatgccctag gtttatatat cacatattat gatacgcttt ttaaaaattt
     1381 taaataataa ttcataaatg cttctggttc ttcaatatgc ggtgcatgtc ctgaatgttc
     1441 gaagattttt ttcgtaacat ttaaaaaatg cgcttccaat ttaaaatgat tcttatcttt
     1501 aatcaaagga tcatgttccc cattaattaa caaagtaggt acacatgtac gtttttttaa
     1561 atacataggt tcatctttat gcaaaattaa tgaacgaatt gccaccgcac tatcatcctc
     1621 agacttaatg ctatatattc tttgtttttg ataccatttc atcgtcttct cttgatttct
     1681 aaacaattta gaaaataaaa gaatatacgc ttcagactta ttaaaacccg ctatatcatc
     1741 ttgatattgt attaacaatt gagtaaagct gtgtattaag tcatctttct tcgatgcaat
     1801 ggtagttaat gattttacac gataagcata tttatctgta aataacttag caactacccc
     1861 acctaaatca tgtcctagaa tatgtgcctc atgaatattt aacttctcca ttagaatttt
     1921 taaatcctca acgtgatcgt ttaaatcgta tgattcactt ttagaagact tgccatgacc
     1981 tcttaaatcg taagtaagta ctttatatga tgatgctagt tgatgttgca aatcttcaaa
     2041 tccagctaaa ttaccatcca atccatgaag taatattata ggatcccctt taccttcaat
     2101 ttgataagtt aattggattt gaggattaat tgtgacttta ttcattgtga cacctccctc
     2161 aataaactat atacccaacc ttcattctat atacgcttcc attttataaa caattttttt
     2221 aaattattta taaattacca aaagttattc caacaataaa aaaactattc atctcaatta
     2281 atacaattaa tcaagtgaat agttttatca tatttataat cttgtacgtg aagataatct
     2341 aattgtgaaa tatataacta gaactactaa taatatccat ataattaatg aaaaaatctg
     2401 ggctgattgt ggattatgaa tcattgcatc gacggttttt tgacaaaata ataacccgat
     2461 tcccgaaaac tcaagtcgct tataaaatat gccggcaatt actaaaataa taccaatgaa
     2521 aaagaaaaat tgatgtccaa aatttgtaat tggtataacg ccccaagtaa tattaaatgc
     2581 ttgtaatata attaataaca aggatatgat agcgataatt gtacccataa tcatttctat
     2641 tttattacta cgcaagtaat tcttttgaga cacccttagg tatacaacaa tagtcattgg
     2701 aaaaatcact aaacaataaa atagactcat tccagta
//
LOCUS       DAMQYT010000248         2723 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMQYT010000248 DAMQYT010000000
VERSION     DAMQYT010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.255"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..361
                     /locus_tag="PGA33_002545"
     CDS             <1..361
                     /locus_tag="PGA33_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDG6474313.1"
                     /translation="TDCKILTLPKDLLEYLCRKHNEIFESLFKKLNETIQFQVEYIMA
                     LRANSAKERIERILQILCLSIGDDNGEFYELKQIMTVQLISNLSGLNRKTTGEIIREL
                     KIENIIYQDKRNWIIKK"
     gene            399..1397
                     /gene="argF"
                     /locus_tag="PGA33_002546"
     CDS             399..1397
                     /gene="argF"
                     /locus_tag="PGA33_002546"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDG6474314.1"
                     /translation="MKNLRNRNFLTLLDFTQKEMEFLLNLSEDLKRAKYAGIEQQKMK
                     GKNIALLFEKDSTRTRCAFETAAYDQGAHVTYLGPTGSQMGKKESTKDTARVLGGMYD
                     GIEYRGFSQRVVEDLAKYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKPYNEINFTY
                     VGDGRNNVANALMQGAAIMGMTFHLVCPKELNPTDELLNRCNDIADKNGGEILITDDI
                     DEGVKGSDVIYTDVWVSMGEPDEVWEKRIKLLEPYRVTKELMKKTGNPHTIFEHCLPS
                     FHDTETIIGKQIQEKYGLTEMEVTNEVFESEQSVVFQEAENRAHTIKAVMVATLGE"
     gene            1417..2346
                     /gene="arcC"
                     /locus_tag="PGA33_002547"
     CDS             1417..2346
                     /gene="arcC"
                     /locus_tag="PGA33_002547"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDG6474315.1"
                     /translation="MSKIVVALGGNALGQSPKEQLDLLKSTSKSLVSLIDKGYEIVIS
                     HGNGPQVGSINLGLNYAAEHKQGPPFPFPECGAMSQAYIGYQMQESLQNELHSMGIDK
                     QVVTLVTQVQVASDDSAFNNPTKPIGLFYTKEQADKFTKEKGYTFVEDSGRGYRRVVP
                     SPQPISIVELDSIETLITHGTLVIAAGGGGIPVIKENEVYTGVDAVIDKDKTSALLAA
                     HLQSDQLIILTAVDHVYINYGKENQRGLDEVSVDEMKKHISDGQFAKGSMLPKVEAAL
                     QFLEKNTKGSVLITSLAGLGDALDGKIGTLIKN"
     gene            complement(2446..>2723)
                     /locus_tag="PGA33_002548"
     CDS             complement(2446..>2723)
                     /locus_tag="PGA33_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDG6474316.1"
                     /translation="TSYAYYQLCEEKGIIRSMSRKGTPADNAPIESFHSSLKSETFYI
                     NNELNHSNHIVIDIVEKYIKNNNNNNNNRIQQKLGYLSPVKYRELIA"
BASE COUNT         1020 a          382 c          504 g          817 t
ORIGIN      
        1 gacagattgt aaaatattaa ctttaccgaa agatttactt gagtatttat gtagaaagca
       61 taatgaaata tttgaaagtc tcttcaagaa acttaatgag actattcaat ttcaagtaga
      121 atatattatg gcgttaagag ctaattcagc taaagaaaga attgaaagaa tactacaaat
      181 tttatgcctt tcaattgggg atgataatgg agaattctat gaattaaaac aaattatgac
      241 tgttcaatta ataagtaatt tatctggact taacagaaaa actactggtg aaataatcag
      301 agaattaaaa atagaaaata ttatatatca agataaaaga aattggatta taaaaaaata
      361 acatgaataa aatattaatt gaaaaggaga tattcaaaat gaaaaattta agaaatagaa
      421 actttttaac tttgttagac ttcacacaaa aagaaatgga atttttactt aatttatctg
      481 aagatcttaa acgcgcaaaa tatgcaggaa tagaacaaca aaaaatgaaa ggtaaaaata
      541 tcgctctact ttttgaaaaa gattcaacac gcactcgatg tgcatttgaa acagcggctt
      601 atgatcaagg tgcacatgta acataccttg ggccaacagg ttctcaaatg ggtaaaaaag
      661 agtctaccaa agatactgct cgtgttttag gtggaatgta tgatggcata gaatatcgtg
      721 gattctcaca aagagtagtt gaggatttgg caaaatattc tggcgttccc gtatggaatg
      781 gtttaacaga tgaagatcat cctacacaag tacttgctga ttttttaaca gctaaagaag
      841 tattgaaaaa accatataat gaaattaact tcacatatgt tggagatgga cgtaataacg
      901 ttgcaaatgc cttaatgcaa ggagcagcaa tcatgggcat gactttccat cttgtatgtc
      961 ctaaagaact aaatccaact gatgaattat taaatcggtg taatgatata gctgacaaaa
     1021 acggtggtga aatccttata actgatgata ttgatgaagg tgtcaaagga tctgatgtaa
     1081 tttacacaga tgtttgggta tctatgggtg aacctgatga agtttgggaa aaacgtatca
     1141 aattattaga accataccgt gtaactaaag aattaatgaa aaaaacaggt aatccacata
     1201 caatttttga acattgctta ccatcattcc atgatactga aacaataatc ggtaaacaaa
     1261 ttcaagaaaa atatggtcta actgaaatgg aagtaacaaa tgaagtattt gaaagtgaac
     1321 aatcagttgt tttccaagaa gcagaaaata gagcacatac aataaaagca gtcatggtag
     1381 caacattagg agagtaaatg aaaggaggat ataaatatgt ctaaaatcgt cgtagctttg
     1441 ggtggtaacg ctttaggaca atcgcctaaa gaacaattag atttattaaa aagtacatct
     1501 aaatctctag taagtttaat cgataaagga tacgaaattg taattagtca tggtaatggt
     1561 ccacaagttg gaagtattaa cttaggttta aattatgcgg cggaacacaa acaaggtcct
     1621 ccttttcctt tccctgaatg tggtgctatg agtcaagctt atattggcta tcagatgcaa
     1681 gaaagtttac aaaacgaact tcattcaatg ggcatagata aacaagttgt tacgctagta
     1741 acacaagttc aagttgcaag cgatgattct gctttcaata accctactaa accaattgga
     1801 ttattttata caaaagagca ggctgataaa tttacgaaag aaaaaggtta tacttttgta
     1861 gaagattctg gacgcggcta tcgtcgcgtt gtcccttccc cacaacctat aagtatagtt
     1921 gagttagata gtatagaaac attaatcact catggaacac tagttatcgc tgccggcggt
     1981 ggtggaattc cagtaattaa ggaaaatgaa gtttatacag gtgttgatgc agttattgat
     2041 aaagataaaa cgagtgcttt attagcagca catttacaat ctgatcaatt aatcatatta
     2101 actgctgtgg accatgttta cattaactat ggaaaagaaa accaaagagg tctcgatgaa
     2161 gtgtctgtgg atgaaatgaa aaaacatatc tctgatggtc aatttgctaa aggaagtatg
     2221 cttccaaaag ttgaagctgc acttcaattt cttgaaaaaa atactaaagg cagtgttttg
     2281 attacatctc tagcaggatt aggggacgct ttagacggta aaataggaac attaattaag
     2341 aattgaaatt ataatacttt agttcaatta cctttataaa caaaagagcc tataatccta
     2401 gggcactgaa cccttaaaac gggaacttaa taaaaacacc atgttctagg ctattaattc
     2461 tctgtatttt acaggggata agtagcctag tttttgttga attcgattat tattattatt
     2521 attattgttt ttaatgtact tttcgacaat atctattaca atgtgattag agtgattaag
     2581 ctcattattg atgtaaaaag tttcagactt tagcgaggaa tggaaacttt ctatcggggc
     2641 gttatcggca ggtgttccct ttcgggacat acttctgata atgccttttt cttcgcacaa
     2701 ttgataataa gcataagatg tat
//
LOCUS       DAMQYT010000249         2685 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMQYT010000249 DAMQYT010000000
VERSION     DAMQYT010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.256"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            127..240
                     /locus_tag="PGA33_002549"
     CDS             127..240
                     /locus_tag="PGA33_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474317.1"
                     /translation="MYFKNSSLNYIPTTFGGEPKETLFYKVSNTLCYVNNS"
     gene            complement(244..327)
                     /locus_tag="PGA33_002550"
     tRNA            complement(244..327)
                     /locus_tag="PGA33_002550"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(291..293),aa:Leu,seq:caa)
     gene            complement(366..440)
                     /locus_tag="PGA33_002551"
     tRNA            complement(366..440)
                     /locus_tag="PGA33_002551"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(405..407),aa:Gly,seq:tcc)
     gene            complement(544..618)
                     /locus_tag="PGA33_002552"
     tRNA            complement(544..618)
                     /locus_tag="PGA33_002552"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(583..585),aa:Gly,seq:tcc)
     gene            complement(626..699)
                     /locus_tag="PGA33_002553"
     tRNA            complement(626..699)
                     /locus_tag="PGA33_002553"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(665..667),aa:Cys,seq:gca)
     gene            complement(705..776)
                     /locus_tag="PGA33_002554"
     tRNA            complement(705..776)
                     /locus_tag="PGA33_002554"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(742..744),aa:Gln,seq:ttg)
     gene            complement(788..860)
                     /locus_tag="PGA33_002555"
     tRNA            complement(788..860)
                     /locus_tag="PGA33_002555"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(825..827),aa:His,seq:gtg)
     gene            complement(863..936)
                     /locus_tag="PGA33_002556"
     tRNA            complement(863..936)
                     /locus_tag="PGA33_002556"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Trp,seq:cca)
     gene            complement(952..1032)
                     /locus_tag="PGA33_002557"
     tRNA            complement(952..1032)
                     /locus_tag="PGA33_002557"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(996..998),aa:Tyr,seq:gta)
     gene            complement(1038..1113)
                     /locus_tag="PGA33_002558"
     tRNA            complement(1038..1113)
                     /locus_tag="PGA33_002558"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1078..1080),aa:Thr,seq:tgt)
     gene            complement(1121..1193)
                     /locus_tag="PGA33_002559"
     tRNA            complement(1121..1193)
                     /locus_tag="PGA33_002559"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1158..1160),aa:Phe,seq:gaa)
     gene            complement(1212..1287)
                     /locus_tag="PGA33_002560"
     tRNA            complement(1212..1287)
                     /locus_tag="PGA33_002560"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1252..1254),aa:Asp,seq:gtc)
     gene            complement(1297..1370)
                     /locus_tag="PGA33_002561"
     tRNA            complement(1297..1370)
                     /locus_tag="PGA33_002561"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1334..1336),aa:Met,seq:cat)
     gene            complement(1385..1477)
                     /locus_tag="PGA33_002562"
     tRNA            complement(1385..1477)
                     /locus_tag="PGA33_002562"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1439..1441),aa:Ser,seq:tga)
     gene            complement(1516..1591)
                     /locus_tag="PGA33_002563"
     tRNA            complement(1516..1591)
                     /locus_tag="PGA33_002563"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1556..1558),aa:Asp,seq:gtc)
     gene            complement(1602..1691)
                     /locus_tag="PGA33_002564"
     tRNA            complement(1602..1691)
                     /locus_tag="PGA33_002564"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1653..1655),aa:Ser,seq:tga)
     gene            complement(1701..1774)
                     /locus_tag="PGA33_002565"
     tRNA            complement(1701..1774)
                     /locus_tag="PGA33_002565"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1738..1740),aa:Ile,seq:cat)
     gene            complement(1803..1876)
                     /locus_tag="PGA33_002566"
     tRNA            complement(1803..1876)
                     /locus_tag="PGA33_002566"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1840..1842),aa:Met,seq:cat)
     gene            complement(1898..1973)
                     /locus_tag="PGA33_002567"
     tRNA            complement(1898..1973)
                     /locus_tag="PGA33_002567"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Ala,seq:tgc)
     gene            complement(1991..2064)
                     /locus_tag="PGA33_002568"
     tRNA            complement(1991..2064)
                     /locus_tag="PGA33_002568"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2028..2030),aa:Pro,seq:tgg)
     gene            complement(2074..2147)
                     /locus_tag="PGA33_002569"
     tRNA            complement(2074..2147)
                     /locus_tag="PGA33_002569"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2111..2113),aa:Arg,seq:acg)
     gene            complement(2166..2254)
                     /locus_tag="PGA33_002570"
     tRNA            complement(2166..2254)
                     /locus_tag="PGA33_002570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2218..2220),aa:Leu,seq:taa)
     gene            complement(2265..2339)
                     /locus_tag="PGA33_002571"
     tRNA            complement(2265..2339)
                     /locus_tag="PGA33_002571"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2305..2307),aa:Gly,seq:gcc)
     gene            complement(2343..2424)
                     /locus_tag="PGA33_002572"
     tRNA            complement(2343..2424)
                     /locus_tag="PGA33_002572"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2388..2390),aa:Leu,seq:tag)
     gene            complement(2428..2503)
                     /locus_tag="PGA33_002573"
     tRNA            complement(2428..2503)
                     /locus_tag="PGA33_002573"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2468..2470),aa:Lys,seq:ttt)
     gene            complement(2509..2584)
                     /locus_tag="PGA33_002574"
     tRNA            complement(2509..2584)
                     /locus_tag="PGA33_002574"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2549..2551),aa:Thr,seq:tgt)
     gene            complement(2601..2676)
                     /locus_tag="PGA33_002575"
     tRNA            complement(2601..2676)
                     /locus_tag="PGA33_002575"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2641..2643),aa:Val,seq:tac)
BASE COUNT          768 a          677 c          621 g          619 t
ORIGIN      
        1 tggttacaga aatttcaaca actttaaagc acgtataatg atgattttca gcttgtacaa
       61 aggagaaaaa agaagacaac caagcccaat aatggactgg ccgcctaata ataaaaactc
      121 taaaagttgt attttaaaaa tagttcttta aattatatac ccaccacatt tggtggagaa
      181 ccaaaagaaa ccttgtttta caaggtttct aatacgttat gttatgtaaa taacagttaa
      241 ttataccggt ggtcggggtc gaaccgacac tccacaagtg gaacgggatt ttgagtcccg
      301 cgcgtctgcc aattccgcca caccggctta atggtaaaca aaaaacttcc ctttggaagc
      361 aattatggag cggaagatag gatttacacc tatacctcgt tccgggaagg aacgtgttct
      421 aaaagttgaa ctactcccgc aaatattaaa ttatggagcg gaagatagga tttacaccta
      481 tacctcattc caggaaggaa tgtattctaa gagttgaaat actcccgcat tattattaaa
      541 ttatggagcg gaagatagga tttgcaccta tacctcgttc cgggaaggaa cgtgttctaa
      601 aagttgaact actcccgcat aaacctggag gcggcaaccg gatttgaacc ggtgataaag
      661 gttttgcaga cctctgcctt accacttggc tatgccgcca ataactgggc tagctggatt
      721 cgaaccaacg agtgacggag tcaaagtccg ttgccttacc gcttggctat agcccattaa
      781 taataagggc ggctgaaggg gatcgaaccc tcgaatgtcg gaaccacaat ccgatgtgtt
      841 aaccacttca ccacagccgc catggcaggg gcagtaggaa tcgaacccac accaaaggtt
      901 ttggagacct ttattctacc gttgaactat gcccctatta aaaataataa atggaggggg
      961 gcagattcga actgccgaac ccgaaggagc ggatttacag tccgccgcgt ttagccactt
     1021 cgctacccct ccataaatgg tgccggccag aggacttgaa cccccaacct actgattaca
     1081 agtcagttgc tctaccaatt gagctaggcc ggctaagaaa tggttcagga cagagtcgaa
     1141 ctgccgacac atggagcttc aatccattgc tctaccaact gagctactga accataataa
     1201 aaatgtaatg atggcggtct cgacgggaat cgaacccgcg atctcctgcg tgacaggcag
     1261 gcgtgttaac cgctacacta cgagacctat aaaatattgc gggaggcgga tttgaaccac
     1321 cgaccttcgg gttatgagcc cgacgagcta ccgaactgct ccatcccgcg ataataaaaa
     1381 ataatggcgg aggaagaggg attcgaaccc ccgcggcccg ttaaggccct gtcggttttc
     1441 aagaccgatc ccttcagccg gacttgggta ttcctccatt attataggta aatcgctatt
     1501 aattataaaa ttaaatggcg gtctcgacgg gaatcgaacc cgcgatctcc tgcgtgacag
     1561 gcaggcgtgt taaccgctac actacgagac cattagtaaa acggaggaag agggattcga
     1621 acccccgcga gccgttaagc ccctgtcggt tttcaagacc gatcccttca gccggacttg
     1681 ggtattcctc caaaattata tggaccttgc aggactcgaa cctgcgaccg aacggttatg
     1741 agccgttagc tctaaccaac tgagctaaag gtcctaaata taattttaca actaataaat
     1801 agtggcggtg gaggggatcg aacccccgac ctcacgggta tgaaccgtac gctctagcca
     1861 gctgagctac accgccttat atagtttgta aataatatgg tggagactag cgggatcgaa
     1921 ccgctgacct cctgcgtgca aagcaggcgc tctcccagct gagctaagcc cccataataa
     1981 ttacagtata tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc
     2041 tctaccaagc tgagctactt cccgtataat taacgcgccc gataggagtc gaacccataa
     2101 cctcttgatc cgtagtcaaa cgctctatcc aattgagcta cgggcgcata tgtttttatt
     2161 gaaaatggtg ccgaggaccg gaatcgaacc ggtacggtga tcactcaccg caggatttta
     2221 agtcctgtgc gtctgccagt tccgccaccc cggcactata aaaatggagc agaagacggg
     2281 attcgaaccc gcgaccccaa ccttggcaag gttgtattct accgctgaac tacttctgca
     2341 tatgcgggtg aagggagtcg aacccccacg ccgtaaggcg ctagatccta agtctagtgc
     2401 gtctgccaat tccgccacac ccgcaaatgg tgagccatag aggattcgaa cctctgaccc
     2461 tctgattaaa agtcagatgc tctaccaact gagctaatgg ctcttccatg gtgccggcca
     2521 gaggacttga acccccaacc tactgattac aagtcagttg ctctaccaat tgagctaggc
     2581 cggcaatatg taagaataaa tggtggagaa tgacgggttc gaaccgccga ccctctgctt
     2641 gtaaggcaga tgctctccca gctgagctaa ttctccgatt taaaa
//
LOCUS       DAMQYT010000250         2652 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMQYT010000250 DAMQYT010000000
VERSION     DAMQYT010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.257"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..752)
                     /locus_tag="PGA33_002576"
     CDS             complement(<1..752)
                     /locus_tag="PGA33_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDG6474318.1"
                     /translation="MAMNEIALFKGVRIQPYQKYIDFMFASVLAFIFLVYRIYILFID
                     YKSVNKKLDILTLFAYFKPHLLYLLIGTAIIFVICKLIAQFLIRFKTKDLAEMIETQG
                     FHNRTVIKKTTEYIPIDYKQTEYDYYPTMFYKRGKKSFVIHIKKDGSRFQDNYLELET
                     IIEPMFNSELVEKKHIGRYLRYEFMPLKYKKRIVMNGEQESNTIFYDTKIAITHQITW
                     DFVKAPHGLVTGITGGGKTYFLFYVIRELFRR"
     gene            complement(806..>2652)
                     /locus_tag="PGA33_002577"
     CDS             complement(806..>2652)
                     /locus_tag="PGA33_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDG6474319.1"
                     /translation="AVIDPTRLSVLEVENDNEKFYTTTVVVDDFPLDMEYTHLFEKAQ
                     NMPFPVECHVKAKIINNEKVKKKFDRAKINHKQQAKEKQDTGDSASEDVQDNLFVLNQ
                     MEREVTGSGVFIEWVFMFVIKSDDYRDCKRKAKRLMKRLKETQIYAVNPLADQLQLFY
                     QCLHGNDLFINKYWLQRTTATGIAENLFGVSQSLGTKTGFYIGRIDKFIRSVSREEAV
                     ASSRDIILFSLLLAAKGIKGAVSDSPHVLITGQTGKGKSFLAKLLWIYTSFFKGQMLY
                     WDPKSEFAEWFDRVTESKEMQEKYPLFINHLKTFKYVTLNYEDSTNYGCLDPIVFLDG
                     IEANEMLKSVFDEIKSFENYHHIETAIYQAISDTVEERENGKKVGSLNVIEKLQNNED
                     EHVKNAGDLLFEKTQNNILKLVFSDGTNPALNIQEKATILQVKGLDMPKADDDTSSYS
                     TSEKNGITLMLLIGKFLEKFGSRRDVQTTIFIDEGWAFSASRQGKKVGKRIKRTGRSE
                     NNSLVFITQSVKDKADDDGGNFGCHFAFDEKDEREDILKSLGLEYSKESPENMEMLKD
                     LKKGQCIFSDFYGRVGKMVVHCPFEEMTEAFRTQEDSASSKAEEKFAM"
BASE COUNT          903 a          513 c          282 g          954 t
ORIGIN      
        1 tgtctacgga ataactcacg aatcacataa aataaaaaat atgttttacc acctccggtt
       61 atacctgtaa ccaatccatg aggtgctttc acaaaatccc aagtaatttg atgcgtaata
      121 gcaatttttg tatcataaaa aattgtattt gattcttgtt cgccattcat aacaatacgc
      181 tttttatatt taagtggcat aaattcatat ctcaaataac gaccaatatg ctttttttct
      241 actaattcag aattaaacat tggttcaata atcgtctcta actctaaata attatcttga
      301 aacctcgaac cgtctttctt aatatgaata acaaaagatt ttttaccacg cttataaaac
      361 attgttggat aataatcata ttcagtttgt ttataatcaa tagggatata ctcagttgtt
      421 ttcttgatta ctgtacgatt atgaaaacct tgtgtttcaa tcatttcagc caaatctttt
      481 gttttaaaac gtattaaaaa ctgagcaata agcttacata tcacaaatat aattgctgta
      541 ccaattaata aatacaataa atgcggttta aaatatgcaa acaacgtcaa tatatccaat
      601 tttttattaa ctgatttata atcaataaac aaaatatata tacgatatac caaaaaaata
      661 aatgctaata cacttgcaaa cataaaatca atatacttct gataaggctg tatacgtacg
      721 cctttaaata aagcaatttc attcattgcc atcaaatcca ctcctttaaa ataaaaagca
      781 catacaattt tgtatgtgct aaaaattaca tcgcaaattt ttcttcagct ttagagcttg
      841 cagaatcttc ttgtgttcta aatgcttcag tcatttcctc aaatggacaa tgtacaacca
      901 tttttccaac acgtccataa aaatcactga atatacattg acctttcttc aaatctttca
      961 acatctccat gttttcagga gattcttttg aatattctaa gcctaaagat tttaaaatat
     1021 cctcacgttc atctttttca tcaaatgcaa aatgacaacc aaagttacca ccatcatcat
     1081 ctgctttatc ttttaccgat tgtgtaataa atacaagtga attattctca gaacgtcccg
     1141 ttctttttat acgcttacca acttttttac cttgtcttga agcactaaaa gcccaaccct
     1201 catcaataaa tatagtcgtt tgaacatctc tacgagaacc gaatttctct aaaaacttac
     1261 caattaataa cattaatgta atcccatttt tctcacttgt tgaataactt gatgtatcat
     1321 catctgcttt aggcatatct aaacctttaa cctgtaaaat agttgctttt tcttgaatat
     1381 ttaaagctgg attcgtacca tcactaaaaa caagttttaa aatattgttt tgcgtttttt
     1441 caaataataa atcgcctgca ttttttacat gctcatcttc attattctgt aatttttcaa
     1501 ttacatttaa tgaaccaact tttttaccat tttcacgttc ttcaaccgta tcagaaatag
     1561 cttgataaat tgctgtttca atatgatgat aattttcaaa agatttaatt tcatcaaaca
     1621 ctgatttcaa catctcatta gcttcaatac catctaaaaa tacaataggg tctagacaac
     1681 cataattggt tgaatcttca taattcaatg ttacatattt aaatgttttt aaatgattaa
     1741 taaatagggg atacttttct tgcatttcct tactttctgt tactctgtca aaccattctg
     1801 caaactcaga ttttggatcc cagtacaaca tttgaccttt aaaaaatgat gtataaatcc
     1861 ataataattt tgctaaaaat gatttacctt tcccagtttg accagtaatt aaaacatgcg
     1921 gactatcact gacagccccc ttaatacctt ttgcggccaa caataaacta aacaaaatta
     1981 tatcccttga acttgcaaca gcttcctcac gtgaaacaga acgtataaat ttatcaatac
     2041 gccctatata aaaacccgtt tttgttccca aactttgaga cacaccaaat aaattctctg
     2101 ctatacctgt tgccgttgtt ctttgtaacc aatacttatt aataaataaa tcattaccat
     2161 gtaaacattg ataaaacaat tgtaactggt cagctaatgg attaactgca tatatttgag
     2221 tttcctttaa tcgtttcatc aaacgctttg ctttacgttt acaatctcta taatcatcac
     2281 ttttaattac aaacataaat acccactcaa taaatacacc tgagccagtc acttctcgtt
     2341 ccatttgatt caatacaaac aaattatctt gaacatcttc cgaagcactg tcgccagtat
     2401 cttgcttttc ttttgcttgt tgtttatgat ttattttcgc acggtcaaat tttttcttaa
     2461 ctttctcatt attaatgatt tttgctttta catggcattc aaccggaaaa ggcatatttt
     2521 gagctttctc aaataaatgt gtatattcca tatctaacgg aaaatcatca acaacaactg
     2581 ttgttgtata aaatttctcg ttatcatttt caacttctaa aacactcaag cgagtagggt
     2641 caattactgc at
//
LOCUS       DAMQYT010000251         2641 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMQYT010000251 DAMQYT010000000
VERSION     DAMQYT010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.258"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            184..627
                     /locus_tag="PGA33_002578"
     CDS             184..627
                     /locus_tag="PGA33_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDG6474320.1"
                     /translation="MKIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLRRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            821..2419
                     /locus_tag="PGA33_002579"
     CDS             821..2419
                     /locus_tag="PGA33_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDG6474321.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDAEIEKWQQQKDSNQ"
BASE COUNT          960 a          395 c          512 g          774 t
ORIGIN      
        1 tgaagacaca aaaatagtgg gcgactcctg tcccccattt gcatgaaata ttaatacttt
       61 ttacaaagtc ttatctttta aaaagaagcc aaactatgat tgtaattaat aaatacggga
      121 atgaattagt aatagatata aagtaatatg accaaaaata taaatgaaat gaaggcattt
      181 attatgaaaa tttatgatga atatagaagt tatgtaatag aagaactgga tgattgtctc
      241 acaatacaaa aaaataatga cactgcatat tacgatgtgc tagaagcgat aaatgattta
      301 tcaaatgatt cattatgtgt gttaaatcat ttatatatta acgaaggaca agaagaaaca
      361 ttcgagcaaa aatttttgcg acgtaataag catttaaaaa acgttgatgg atttaaagca
      421 ttaaggtttt taagacctag aacagctggt agacactata tcattattac gctgtgggaa
      481 aataggcaag cattttatca ttggcaaaat tcagctgagt acaagcatac acacaagcat
      541 cgtggcactt cgaaaggtgc agatgtaaaa ataattaatc gtgaattatc atacaatatt
      601 cgtattgaat tagctgatat ggtctagccc gagatatcaa ccctcaatca tttgaaagtc
      661 cttgaatgct acgtcatgtc aaagtgtagt gtcgaggact ttggtattta accctattta
      721 attgaaaatt ctgtaaagat aggcgtatga tgtatataat tcatttataa aattacaatg
      781 ttcatcacag gaaaagtgga tgtatgaaag gaagttagtt atgcaaaagt cagctctatt
      841 cgcgcctgaa aaatataaca ttatttcgga aatcgaaaaa tatagccaca taccagataa
      901 aaaggcaatt ttatatcata atactgaagg cgaagatata tcagtcactt accaacagct
      961 cattgaacag tctaataaag taggtaatgt tttagcatcg cacggtttat ctaaaggcga
     1021 taaagtactc attatgatgc cacgatctat tgcgacgtat gaattatata ttgctgcttt
     1081 aaaattaggt gttgcaatca ttccatgttc tgaaatgcta agaactaaag atttacaata
     1141 ccgtatcaca catggtgaaa ttaatgcagt tattgcacta gagccattca cagttgaatt
     1201 tgaaaaaatt aaagaatatg atgcgttaac taaatttgtt attgccggtc aaaaagatgg
     1261 atggatttca cttgaagctg aaaaagaaaa cgcaagtaac aaactagaaa tggcagatac
     1321 gacgcgtgat gatatggcga ttctgtcata tacatctggt acaactggca atcctaaagc
     1381 cgttacacat tgtcatggat ggggatttgc acatttacaa atggcaccaa aacattggtt
     1441 atgtattaat gaagatgact tagtttgggc aacagcagca ccaggttggc aaaaatgggt
     1501 atggagtcct ttcttatctg tattaggttc aggtgcaact gcttttgtat ttaatggaag
     1561 gttcagtcct gaaacatact tagaattgct tcaaaaatat caaattaatg ttttatgttg
     1621 tacaccgaca gaatatcgta tgatggctaa attacagaac ttaaatgatt atgacttaac
     1681 gcatttacat agtgccgttt ctgcaggtga gccactaaat cgagaagtag ttgaacaatt
     1741 caagaaatac tttaatttaa ctgtaagaga tggatatggc cagactgaaa gtaccttgct
     1801 tataggcttt ttaaaagata caccacagag gattggatca atgggtaaag gcattccggg
     1861 tagttcggtc actgtagtag atgatgaggg gaatagcgta cctgctaata cgaaagggaa
     1921 tattgcagta ccacttgatt tgccagcact tttcaaaggg tattataaag agccagaacg
     1981 tacggcgaaa gcgcaaacag gtaaatacta catcactggt gacttagctc atgtagatga
     2041 tgatggttat ttctggtttg aaggacgtcg agatgacatt attattagtt caggctatac
     2101 gattgggcct tttgaagttg aagatgcgtt aacgaatcat ccagccgtta aagaatgtgc
     2161 tgtagtagca aaacctcatg atatacgagg aaatattgtt aaagcattcg ttattttaca
     2221 ggaccatact gcgggtgatg atacgttagt taaagaacta caacaatttg ttaaaaatga
     2281 agttgcacca tataaatatc cgagagagat tgaattcgtt gacgacttac caaaaacaaa
     2341 ctcaggtaaa attcgtcgtg ttgaactacg agatgctgaa atagaaaaat ggcaacaaca
     2401 aaaagatagc aatcaataaa catagcaaag cagtccaatg cgtaaggtat gatgatacgc
     2461 actggactgc ttgtttattt atgatgttct ttgtcgtcta gttataaata ttaaaattag
     2521 tttagaaaca agcgcaacca gtattaagaa tgagaaaata taagatgtta aacctcgaat
     2581 atcccagttt aataaaaaca cattactatc accggcatta agttgaaact cagtatgtgt
     2641 t
//
LOCUS       DAMQYT010000252         2631 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMQYT010000252 DAMQYT010000000
VERSION     DAMQYT010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.259"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            1173..2042
                     /locus_tag="PGA33_002580"
     CDS             1173..2042
                     /locus_tag="PGA33_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDG6474322.1"
                     /translation="MEMSRLNLTDDNDIDEIIAWRGEQEELEQKRDTYKKRYHEFEME
                     IARLESLTKDKELLDSDKLKDEYELKKGKMNTLIDEYSAVHYQCQNNINKTQSIVSHI
                     NYLNQELKDQQEIFQLAEIVSGKNNKNLTLENFVLIYYLDQIIAQANLRLATMSDNRY
                     QLIRREAVSHGLSGLEIDVFDLHSNKSRHISSLSGGETFQSSLALALGLSEIVQQQSG
                     GISLESIFIDEGFGTLDQETLETALDTLLNLKSTGRMVGIISHVSELKNRIPLVLEVK
                     SDQYQSSTRFKRN"
     gene            complement(2113..2475)
                     /gene="mscL"
                     /locus_tag="PGA33_002581"
     CDS             complement(2113..2475)
                     /gene="mscL"
                     /locus_tag="PGA33_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDG6474323.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
BASE COUNT         1086 a          329 c          420 g          796 t
ORIGIN      
        1 ttgctaatag caaaaaagat atttctaaaa ccaataataa aatattgaat cttgattgtg
       61 ataagcgaaa catagacaaa gagaaaaaaa tgttagaaga aaatggagat ttaattgaaa
      121 gtaaaatctc ttttattgat aaaactagag tattatttaa cgatattaat aagtatcaac
      181 aaagttatct caatattgaa cgcttgagaa ctgagggtga acaattaggt gatgaattaa
      241 atgatctaat taaaggttta gaaacggtcg aagattcaat aggtaataac caaagtgatt
      301 acgagaaaat tatcgaacta aataatacga taacgaacat aaataatgaa attaatataa
      361 ttaaagaaaa tgaaaaagct aaagctgaat tagataaact attaggtagt aagcaagagt
      421 tagagaatca aattaatgaa gaaacatcta tattgaagaa tctcgaaata aaattagatc
      481 gctacgataa aacaaaattg gacttaaatg ataaagaaag ctttataagt gaaattaaat
      541 ctgctgtaaa tattggagat caatgtccga tatgtggtaa tgaaattcag gatttagggc
      601 atcatattga ttttgacagt attgctaaac gtcaaaatga aattaaagaa attgaagcaa
      661 atatccacgc tataaaatcg aatattgctg tgcataattc tgaaattaaa tttgttaatg
      721 aaaaaatatc gaatattaat attaaaacgc aaagtgattt ttcacttgaa gtattgaata
      781 agcgtctgct agaaaatgaa aatgcattga ataatcaaag agatcttaat aaatttatag
      841 aacaaatgaa agaagaaaaa gataatctaa cgttgcaaat tcataataaa caattgcgtc
      901 taaataaaaa tgaatctgag ttgaaattat gtcgagatct catcactgaa tttgaaacac
      961 tctcgaaata taataatatc actaattttg aggtggatta taagaagtat gttcaagatg
     1021 tgaatcagca tcaagaactc tcaaaggaga ttgaagataa gttaatgcaa ttgtctcaaa
     1081 gaaagttaat tgagcaaaat aatctaaatc attatgaaaa tcaactagaa acttacaata
     1141 atgacttaga attgaatgaa cagtctattg aaatggaaat gtcgaggctg aatttaactg
     1201 atgacaatga tatagatgaa ataatagcct ggaggggcga gcaagaggaa ttagagcaga
     1261 aaagggatac ttataaaaaa cgttatcatg aatttgaaat ggaaatagct aggttagaat
     1321 cattaactaa ggataaagag ttattggact ctgataaatt aaaagatgaa tatgagctaa
     1381 aaaaaggaaa gatgaataca ctgatagatg aatactctgc tgttcattat caatgtcaaa
     1441 ataatattaa taaaacacaa tctatagttt cgcatattaa ttacttaaat caagaattaa
     1501 aggatcaaca agaaatattt caattggctg aaattgtcag tggtaagaat aacaaaaatc
     1561 ttacattgga aaactttgtc ttaatttact atttagatca aattattgct caagcaaatc
     1621 tgagattagc aacaatgtca gataatcgat accaactaat taggcgagaa gcggtttctc
     1681 atggtcttag tggcctagaa attgatgtat ttgatttgca ttcaaataag tctagacata
     1741 ttagctcgtt atcaggtgga gaaactttcc aatcgtcgct tgcattagct ttagggttaa
     1801 gcgaaattgt acagcagcaa tcaggaggta tttcactaga atcaatattt attgatgaag
     1861 gattcggtac attagatcaa gaaacgcttg aaacagcgtt agacacttta ttaaatctta
     1921 aatcaactgg tagaatggtt gggattattt cacatgtgag cgaattgaaa aatagaatac
     1981 ctttagtttt agaagtgaaa tcagatcaat atcagagttc aacaagattc aaaagaaatt
     2041 aaaacataaa aaatagagat ggtactttgg aatgtataga acaaccttag cccatctctt
     2101 tttatttaaa aattattttt tctcacgtaa taaatctctg atttcagtta ataacacaac
     2161 attttcttcc acaactgctt cttcttcggc ttcttctttc ttcattaatg tatttgcaat
     2221 cttaacaaag atgaataaag caaacgcgat gataataaag tcgataacag attggataaa
     2281 taaaccgtat ttaatacccc agaatgacca ttctttagca aaatcaactg atccgaaaat
     2341 tttaccaatt aatggcataa tgatattttc tactaatgaa gatataatct tgttgaaagc
     2401 tgcacccatc acaacagcaa ttgctaaatc taagacgtta ccttttaagg cgaactcttt
     2461 gaattctttt aacattacac tcaacctctc ttttttaaat gtaacctcaa ttatacaaca
     2521 aataaaaaaa ttagaaaata gattattcaa gaaaatttga ttatcgcaaa gacctttgtt
     2581 atcctataaa ggtgcgtgag ttattgaata aaatgtttag ataatatttt t
//
LOCUS       DAMQYT010000253         2572 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMQYT010000253 DAMQYT010000000
VERSION     DAMQYT010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.260"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(258..1466)
                     /locus_tag="PGA33_002582"
     CDS             complement(258..1466)
                     /locus_tag="PGA33_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDG6474324.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
     gene            complement(1459..1626)
                     /locus_tag="PGA33_002583"
     CDS             complement(1459..1626)
                     /locus_tag="PGA33_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474325.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            complement(1601..2452)
                     /locus_tag="PGA33_002584"
     CDS             complement(1601..2452)
                     /locus_tag="PGA33_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDG6474326.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
BASE COUNT          932 a          423 c          319 g          898 t
ORIGIN      
        1 atattattaa ttaacagccg attatgagtg cttcttaata tattaaaaat accacttatt
       61 tttattttcg acataataat gaagtactaa tcaagtggca aatactcgtt tcattatgca
      121 tgtgctttat aaacggctaa actttcgtaa aaatttataa gctaaacatg tgctgataat
      181 cataaatgta actgcccaac attataagca ccataaaaaa taccaacaaa catatagtca
      241 ttggtatttt ttattactta ttcaactatt ttattacttt ctttaatatc atattcacct
      301 acatcattac catagaaacg attgtatcga cgtttaaata attggaataa atgagtaact
      361 aatactttta aaatagcata tcccggaata cctaaaataa cgcctacaat accaagcaat
      421 ttgcctgcac acagtaaaat gaaaatgatt gtaagtggat gaatcttaag tgttttaccc
      481 atgatatttg gtgaaatgaa gtgcccttca acaaattgta ctaaagtcca tactactgct
      541 aattttaaga gcatccacgg cgatgttata gcagctatta caatagctgg agaaatcgct
      601 atagtaggcc ctaaatatgg tacaacactt gtaactgccg caatactagc taatactaag
      661 ctatatttca acccgataac cgaataaccg ataaacaaca gtataccgat acagaatgaa
      721 acgataattt gtccttgaat gtatgaacca acttgaacac tcattttttc aagtagatca
      781 tgaaaatctt ttcggaattt cggtggcata atattcgttg aaaattcttt gaagtgatgt
      841 ccatctttaa gcataaagaa tagtacaaat ggtgttgtga caatcacaac gccaatatta
      901 gcaatcgttt ctgcaaacgt tgcaatttta gacccaaagc catctgtaaa ttcacttaac
      961 atagatggta ttttcttagg taaagaattt aaccaatcat taatttgact ataatacgaa
     1021 gagaaaaacg tattatttgc tattttatca atagaattaa ttaatttttc tagatattgc
     1081 ggtgaatttt taactaaact atctacttgc gaaccaataa taggtatcaa taaattaaca
     1141 attaacgtaa taacacctac tacagctaga taaataatag aaatacctgc aactcttggt
     1201 attctataac gttccatcat atttacgatt gggttaaata gatagaatag aattaaagag
     1261 acgatgatag gtgctgctat cgtcttaaaa acgatgataa aaggatcaaa aacatatgat
     1321 actttttcga aaataaaaat aacaataccc aacaatacta gcgctattaa tgagaagact
     1381 aaatcattcc caccaacaaa cttcatatac cgtgattcgg taaattttaa aaaattcttt
     1441 cttatattct tttcattttc attcaattat agattcacca ccacttaaaa tattcataag
     1501 atgttgtata aaaacagtcc acttatgtaa aattactaac atcaatgata ccatacaaat
     1561 accctttaca gtaacgaaac gcccgtgtaa tacacttaat ttaggattga ttaatccttt
     1621 cttcaaaatg atgaatgtgt aatgttaaat atataatttc agactcactg atattaacat
     1681 caaattgttt ttgtatcata gttaaaattt tatatgctgt gttatagcaa atcggatagt
     1741 gatttttaat catagacaca aaatcatctt gtgcatgtat atattctttt cttcttaatc
     1801 ggcgaattaa aaattgtacg tgccttataa aacgttggta ttgtaatgat tccttatcaa
     1861 cagttgtcac aaggtctgat tcaatgatat ctataccttt tttaataaca ttattgatca
     1921 aggtcatctc atgcatagat aaatcttctg tattagatgc aatatgtaat gcaataaatc
     1981 ctatctcatc ttcaggaaaa tgtacatcta atgctgcatt taactgatca atcacctgtt
     2041 tagcaatatg atatgcatca ctatataact gcatagtttc cataacaaat ggattgctaa
     2101 taacttgatt ttgttttaag cgtttataag caaatataat atgatccgtt aatgaaacta
     2161 caagttgttt tgaatcaaca ttcatcgcag tattagaaat aaaattcaac gaatcaataa
     2221 ttacttgtaa tacattatca tcagcgattt caactaaact tttataatgt gctttttgtt
     2281 gctcactttc taatttataa attttctcta ttgtaatagt ttggtcgttt aacgccattc
     2341 cctctttttt gttaaaacca atacctttac cgattaaaat aacttcttga tcattattag
     2401 tacatactac gacattgttg ttcaatgttt tagtaacaat atattctccc attatcatca
     2461 cctatttttc tattattaag attatatatc ggaaatgtct aacttgaaag tataataatt
     2521 taaatacttg tgttctatta cacgcctatc ctatatgata tatcttaatt ta
//
LOCUS       DAMQYT010000254         2551 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMQYT010000254 DAMQYT010000000
VERSION     DAMQYT010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.261"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            33..524
                     /locus_tag="PGA33_002585"
     CDS             33..524
                     /locus_tag="PGA33_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDG6474327.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            598..1956
                     /locus_tag="PGA33_002586"
     CDS             598..1956
                     /locus_tag="PGA33_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474328.1"
                     /translation="MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQAEADKSVAVSNKESKAVALKAQQAAI
                     KEEASANNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEEPKAVALKAQQAAIKEEAS
                     ANNLSDISQEAQEVQEAKKEAQAEKDSDTLTKDASAAKVEVSKPESQAERLANAAKQK
                     QAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNIDNKDTVKQK
                     EAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQKKQHN
                     KKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKAKSKIEKRTF
                     ND"
     gene            2091..2195
                     /locus_tag="PGA33_002587"
     CDS             2091..2195
                     /locus_tag="PGA33_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474329.1"
                     /translation="MRSFHVFMLFETSLDNNSDIAHFKLKMEKYSIIK"
BASE COUNT         1096 a          346 c          447 g          662 t
ORIGIN      
        1 cattattaca tgttaattag gaggcgtttt taatggattg gattttacca attgctggaa
       61 ttatcgctgc gattgcattc ttaattttat gtatcggtat cgtagctgta ttaaattctg
      121 ttaagaaaaa cttagattat gttgcaaaaa cacttgacgg tgtagaaggt caagttcaag
      181 gtattactcg tgaaacaaca gatttacttc ataaagtaaa ccgtttaact gaggatatcc
      241 aaggtaaagt agatcgttta aactcagttg tagatgctgt taaaggtatc ggtgactcag
      301 tacaaacgtt aaacagctct gtagatcgtg taacaaattc aattacacat aatatttctc
      361 aaaatgaaga taaaatctca caagttgttc aatggtcaaa tgttgcaatg gaaattgcag
      421 acaaatggca aaatagacac taccgtcgtg gaagtgcaaa ttacaaagct aataatgtag
      481 caactgatgc aaatcatagc tatacttcta gagtagataa ataattaata attaaacaaa
      541 tgcacttaaa tgaggttgtt acttatttaa gtgcatttgt tataggagga tatacaaatg
      601 acgcagcaac aaaatgataa aagaacatta aaaaataaac acacttatca aaatgaacca
      661 ctaccaaacc gtaaagattt tgttgttagt tttataactg gcgcgcttgt tggttcagct
      721 ttaggtttgt attttaaaaa taaagtttat caaaaagcag atgatttaaa agtcaaagaa
      781 caagaactgt cgcaaaagtt tgaagaaaga aaaacgcaac ttgaagaaac ggttgcctat
      841 acaaaagaac gtgttgaagg atttttaaac aaatctaaaa atgagcaagc ggcattaaag
      901 gcacaacaag cagcgataaa agaagaagca agtgcaaata atttaagtga tacatcacaa
      961 gaagcacaag agattcaaga agctaaaaga gaagcacaag cagaagcgga taaaagtgtg
     1021 gctgtatcaa ataaagaatc aaaagcagtg gcattgaaag cacaacaagc agcgataaaa
     1081 gaagaagcaa gtgcaaataa tttgagtgat acatcacaag aggcacaaga gattcaagaa
     1141 gctaaaaaag aagcacaagc agaaacagat aaaagtgcag ctgtatcaaa tgaagaacca
     1201 aaagcagtgg cattgaaagc acaacaagca gcgataaaag aagaagcaag tgcaaataat
     1261 ttaagtgata tatcacaaga ggcacaagag gttcaagaag ctaaaaaaga agcacaagca
     1321 gagaaagaca gtgacacatt aactaaagat gcaagtgcag caaaggtaga agtatcaaaa
     1381 ccagagtcac aagctgaaag attagcaaac gctgcaaaac agaagcaagc taaattaaca
     1441 ccaggttcaa aagagagtca attaactgaa gcgttatttg cagaaaaacc agttgctaaa
     1501 aatgacttga aagaaattcc tcaattagtt actaaaaaga atgatgtatc agagacagag
     1561 acggttaata tagataataa agacactgtt aaacaaaaag aagctaaatt tgaaaatggt
     1621 gttattacac gtaaagctga tgaaaaaaca actaataata cagctgttga caagaaatca
     1681 ggtaaacaat ctaaaaaaac aacaccttca aataaacgaa atgcatcaaa agcatctaca
     1741 aataaaactt caggtcagaa aaagcaacat aataagaaat catcacaagg tgcaaagaaa
     1801 caaagtagtt caagtaagtc aactcaaaag aataatcaaa ctagtaataa gaattcaaaa
     1861 acaacaaatg ctaaatcatc caatgcatca aaaacgccaa atgctaaagt tgagaaagct
     1921 aaaagtaaaa tagagaaacg tacattcaat gactaagatt actaaaatcg ttgaataatc
     1981 aatcatagca gtaagctatg taatatattt ttaaaaattg aatttcaaaa taaaaggtag
     2041 gcattcaggt gcttaccttt tttgatatca tcagtttatt ttgaaaggca atgcgatcat
     2101 ttcatgtatt tatgttgttt gaaacatcgt tagataacaa tagtgatatt gcacatttta
     2161 agctgaagat ggaaaaatat tcgataatca aataaacaat gaattttaga aggtacaatg
     2221 acgttcacta atttaaatat agctgaatgt gttggtgagt gatgttcact atagatttat
     2281 attaatatac aaagacaaag gttgttaatt tttattaagc gttaggttga atgtatgaga
     2341 attttagatt tataatagaa gatagaaacg aaaatttttc ttaaaagcag taatgttgac
     2401 tcaaaataag ctataataat gacacttatt taattgatta acatttgcta ataaatatca
     2461 atatagaata taactttcca ataatgactg agaaaatcga aatgtcagtc tcgaatcata
     2521 taattagaaa attgattatt ttctgtcaat t
//
LOCUS       DAMQYT010000255         2539 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMQYT010000255 DAMQYT010000000
VERSION     DAMQYT010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.262"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            237..524
                     /locus_tag="PGA33_002588"
     CDS             237..524
                     /locus_tag="PGA33_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474330.1"
                     /translation="MEKGIFNYDNANVLKLDTNQLNENIKVIDDIFKNYEQIEPTIEV
                     ENGNTKLKLNGYFIASIISPLNLNKLNNLYVEEEFYHTYNELIVKYTEVKE"
     gene            530..2011
                     /locus_tag="PGA33_002589"
     CDS             530..2011
                     /locus_tag="PGA33_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDG6474331.1"
                     /translation="MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYK
                     GNYDEIIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEI
                     EESKGISRYFDITTGTYIAYIRKSKSEKVIDFFKDNKRIERFSFIDNKVHMKETFNVD
                     NKVCYQVFYDEKGYPYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDS
                     KDSIMICDGPGSFPKMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKI
                     NGVIVLTEAQRLDILNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRID
                     LLIEVAELVVKKDNAVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLED
                     FKLVVSTSQYEGQGLSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNINDMA
                     DKILQLVNNDVLAAEFGSKARENIIEKYSTESILEKWLNLFNS"
     gene            complement(2100..2456)
                     /locus_tag="PGA33_002590"
     CDS             complement(2100..2456)
                     /locus_tag="PGA33_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDG6474332.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
BASE COUNT         1042 a          276 c          403 g          818 t
ORIGIN      
        1 gaagaatttg aaattaaatt gaattgtttt tggtagttgg tatatttata acactctgca
       61 aaagaatgta gcaaaattag ataaattaga ttatttaaaa ttatgcgtga tattattatt
      121 tttatcatgc aaataattat aataacaata tttaattatt gtatttacag ttgattaata
      181 gcgttgtgaa gtataaaatt attttgtaaa acaattttgt tattaagagg tgcgctatgg
      241 aaaaggggat tttcaattac gataatgcga atgtattaaa attagatact aatcagttaa
      301 atgaaaacat aaaagtcatt gacgatatat ttaaaaatta tgagcagata gagccaacta
      361 tagaagtcga aaatggaaat acaaaattaa aattaaatgg atattttatt gcgtcaatta
      421 taagcccatt aaatttaaat aaacttaata atttatatgt tgaagaagaa ttttatcata
      481 catataacga attaattgtt aaatatactg aggtaaagga ataattataa tgaaaaaaat
      541 atttatgatg gtacatgagt tagatgtgaa taaaggtggt atgacctctt cgatgttcaa
      601 tagaagtaaa gagttttatg atgcggacat acctgctgat attgttactt tcgattacaa
      661 aggaaactat gatgaaatta ttaaagcttt gaaaaaacaa ggtaaaatgg atcgaagaac
      721 gaaaatgtat aatgtatttg agtattttaa acaaatttca aataataaac attttaagtc
      781 taataaattg ttatataaac atatttcaga aagactaaaa aatacgattg aaattgaaga
      841 gagtaaaggt atttcaagat attttgatat aacgactggt acatatattg cctacattag
      901 aaaaagtaaa tctgaaaaag tgattgattt ctttaaagat aataaacgaa ttgaacggtt
      961 tagttttata gataataaag tgcatatgaa ggaaacattt aatgtagata ataaagtttg
     1021 ttatcaagta ttttatgatg aaaagggata cccatatatt tcaaggaata ttaatgctaa
     1081 taatggtgct gtaggtaaaa cttatgtgtt agttaataaa aaagaattta aaaacaattt
     1141 agcactgtgt gtttactatt tagaaaaact aataaaagat tctaaagata gtattatgat
     1201 ttgtgatgga ccagggagtt ttccaaaaat gtttaataca aatcataaaa atgctcagaa
     1261 atatggcgtt attcatgtta atcatcatga aaatttcgat gatacgggtg catttaaaaa
     1321 aagtgagaaa tatattattg agaatgcgaa taaaattaac ggtgtaattg tattaacaga
     1381 ggcacaaaga ttagatattc ttaatcaatt tgatgtagaa aatattttca ctattagcaa
     1441 ttttgttaag atacataatg ctccaaaaca ttttcaaact gaaaaaatcg taggtcatat
     1501 ttctagaatg gtaccaacga agcgaattga tttgcttatt gaagtggctg agttagtcgt
     1561 aaaaaaagat aatgctgtta aatttcatat atatggagaa ggatctgtca aagataaaat
     1621 agctaaaatg attgaagata aaaatttaga aagaaatgtt tttcttaaag gatatacaac
     1681 aactccacaa aaatgcttgg aagattttaa attagtcgtt tctacatctc aatatgaagg
     1741 tcaagggtta agtatgatag aagcaatgat ttctaaaagg cctgttgttg cctttgacat
     1801 caaatacgga ccaagtgatt ttatagaaga taataaaaat ggttatttaa tagaaaacca
     1861 taatattaac gacatggctg ataaaatact tcagcttgtt aataatgatg tattagcagc
     1921 ggagtttggt tcgaaagcga gagaaaacat tatagaaaaa tattcaacgg aatcaatatt
     1981 agaaaaatgg ttaaatcttt tcaatagcta aatgaaaaaa tcctaggtat tcatgagtac
     2041 ctaggatttt tacgcatgat aaatacaaaa aaagagagga tagtcctctc aaacaatagt
     2101 taataaaatt gtagccattt cttccagctg taactactaa tacaagctaa gttcattacg
     2161 atatgtatga tttcaaattc ataaccactt acatatcctc ttgtaagttt aagtgtgaag
     2221 attgagccta acataacaca aatataaaaa acattaacat aaggcgttaa aaagccgata
     2281 ataagtgcta aacctccaac aacttcacca attgacatca atactgcgat ggtaggtggt
     2341 aaacctaaag agcctaagaa gtgaattgtt ccatctaaat caacaaattt caaagtacca
     2401 tgcactaaga atgatgttgc taacatccat ctaattaaaa gcatacctaa tttcatcatg
     2461 tcacctccaa cattcatatt tttaaaacat agtccaaatt atatttgtaa acatgtggaa
     2521 agtaaagatt ttttataag
//
LOCUS       DAMQYT010000256         2527 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMQYT010000256 DAMQYT010000000
VERSION     DAMQYT010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.263"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(4..1176)
                     /locus_tag="PGA33_002591"
     CDS             complement(4..1176)
                     /locus_tag="PGA33_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDG6474333.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQQINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSAIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            complement(1601..>2527)
                     /locus_tag="PGA33_002592"
     CDS             complement(1601..>2527)
                     /locus_tag="PGA33_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474334.1"
                     /translation="TQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHFMWIIVSY
                     GISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRRALPLTKQ
                     ELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAKRDKFKKK
                     VNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFVSGDWKLS
                     QTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLTEFINIMI
                     HGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
BASE COUNT          853 a          433 c          320 g          921 t
ORIGIN      
        1 aaattaatgt ctaatattta caaaaacagc ttttaaatat ttagatggct tataatgtgg
       61 atgcgtttta aaatcttttg gtaatcccat aacttcagtt aattcataat caacgccact
      121 ctcttcaagc gtctttttaa tagtattttt aaattgcttt aatggatata cacttgcgtt
      181 tgtacataac aataatgttc cttcagatga taagatattt aaggcgccat taattaattt
      241 gtcataatct ttttgcactg aaaatgtacg ttttttgtta cgcgcaaagc taggtggatc
      301 aatcacgatc gtgtcataac tatgtccatg tcgtgcagca tatttataga aatcaaaagt
      361 gtccatgaca taaatatatt gggatttagg atcaatagca tttaatccaa aattttcttc
      421 agttaaacta cgagaacgat tagccaaatc tacacttgtt gttgaagatg cctcacttgc
      481 tgctattgca gaaaaagcac ctgtataact aaataagttt aaaacatggc gttctttggc
      541 atattgatcc cttaatttct tgcgcacttc tttttgatct aaaaagatac ctgtcatcaa
      601 accatcttca aggtctacat tataaaatgt gaagttttct tcgataacaa ttggaaactc
      661 aggtgcatct ccttcaacaa aaccaccact atattcgctg tctttaaatc ttactttttc
      721 gtaaatagat ttataatcaa atacttttct aaccgcttca agaatggcat atttaaattt
      781 ataaatacct tttgagtacc attgaatcaa caaatgacca tcgtaattgt cgattgttaa
      841 tcccccaaca ccatcacctt cagcattaaa taatctaaaa gcatttgttc catctatatt
      901 aaaataataa tcacgttctg ctaatgcagt attaaacaat tttacaaaga aagctgtgtt
      961 gatttgttga gctttatcgt atgttagaac ccaacctaat cctttatgtt ggcgaccaac
     1021 ataagccgtt gcgacatatt gtgatttatc agtcacaatt tgaaaaatat ctccttcttt
     1081 taaatgatct tgtgaataga tatcctcttc ttcaattaaa ggatatccat taaaatattt
     1141 tgtttctttg cctttgttca gagttgctat tttcatctga caacttcctt tctgtgtttc
     1201 gttgtagttt tatatagatt aaattgtttg ccaaatttca atatcatcta cttctaatct
     1261 tactgttgat tttaacaaat tagtaattcg aatgatatat gttaaatttc tacaaaggaa
     1321 atactatata tcgtttcgca aattccataa gtcgcttcaa ctatttacta gttttcttga
     1381 ttttacaaat ggataacttt tcaactattt tatttgcact aaaactttgt atacaatttt
     1441 caatacttaa ttcacatcac ctgaatcaat caaatctagt tacaaaaaaa gtactatcca
     1501 taaacttcat tgacctgtat ctaagtaagt tagagatata tctaacttat agcgtacaca
     1561 tcaaaatgtt taatagatag tacttttata atgcttttaa ttatggtcgc tctcgtaatt
     1621 gtaacgaata agaaatgatg attgccaata acattaatag taatgttggc gtaatgaaca
     1681 ctgaaagtaa tagtccatga atcattatat ttataaattc cgttaacaat ttaaagaata
     1741 aaatgctccc cataaataat tgtaaataat aaaacttacc tttcaagtgg aaagttaacg
     1801 ttgtagcgat atatgcgata ataatgaata acagaattaa taatgtaacc cattcaataa
     1861 catattgtgt ctgtgataat ttccaatcac cagatacaaa taatgcatta cgattattaa
     1921 attctaaaaa tgtgaataaa aagatgacga caccacaaaa tagttcgaca tacattggtt
     1981 taacccattt cggttttctc atccaatcag gaatatcgtc ttcttgaata tcgataatat
     2041 cattaacttt ctttttgaat ttatctcttt tagctctaat tttatctaaa tcgacacttc
     2101 ttcttgtctt acgtgcatat tctgtagtct tatcttggag ttgtttcaag tgaattttag
     2161 tttgattcgt gacattccct ttacttgttc ttgataattc tggctcctct tcacctaatt
     2221 cttgctttgt taaaggtaaa gctcggcgca agaacaagaa attccaaata gccatttgac
     2281 caagtaacca cataattata aagaaagcat tgacacttaa gatatcaatt gactctattt
     2341 cattagcatc aattctgcca tacaatgcaa tttgtgtaat taagtaactt ataccataac
     2401 tcacaattat ccacatgaaa tgatttttta cattttcctg attcaattca tctttataga
     2461 cgatatatcc tatatgaatt gtaaatggaa taatgaaaat cactgcaaag aatccataaa
     2521 cttgagt
//
LOCUS       DAMQYT010000257         2526 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMQYT010000257 DAMQYT010000000
VERSION     DAMQYT010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.264"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..180
                     /locus_tag="PGA33_002593"
     CDS             <1..180
                     /locus_tag="PGA33_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HDG6474335.1"
                     /translation="EIYEFLNDFIQSHQTKTIINITHNLSDLSALPGKYFIFKDLQIE
                     EYQSKEEVINDYINL"
     gene            186..917
                     /locus_tag="PGA33_002594"
     CDS             186..917
                     /locus_tag="PGA33_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HDG6474336.1"
                     /translation="MSTLKLEFKKSISNKIIYTLVVLFTFLFLLGYFLPIGIDKVKSL
                     SYSQFFFSSYTVATQLGFLLFSFVIAYFINKEYSNKNILFYKLIGDNIFTFFYKKVAV
                     LFFECLVFIILSITIISIIYSDFSHYLLLIILFSLVILQYILVVGTISMVSPNILISL
                     GISIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESTFMCPTEIINIVS
                     FFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(1077..1697)
                     /gene="srtA"
                     /locus_tag="PGA33_002595"
     CDS             complement(1077..1697)
                     /gene="srtA"
                     /locus_tag="PGA33_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HDG6474337.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVGVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            1891..2382
                     /locus_tag="PGA33_002596"
     CDS             1891..2382
                     /locus_tag="PGA33_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDG6474338.1"
                     /translation="MIRYAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNRWLDLAFYELD
                     LQD"
BASE COUNT          796 a          411 c          375 g          944 t
ORIGIN      
        1 gaaatttatg aatttttaaa tgattttatt caaagccatc aaactaaaac tattatcaat
       61 attacacata atttatccga tttaagtgct ttgccgggta aatactttat ttttaaagac
      121 cttcaaatag aagagtacca atcaaaagaa gaagtcataa atgattacat taatttataa
      181 ttgaggtgtc cactttgaaa ttagaattta aaaaaagtat ttccaataaa attatttaca
      241 cgcttgttgt gctattcaca ttccttttct tacttgggta ttttttacca ataggtatag
      301 acaaagttaa aagtttaagc tatagccagt ttttcttcag ttcatatact gttgcaacgc
      361 aattagggtt cttactattt tcatttgtta ttgcatattt tattaataaa gagtattcga
      421 ataaaaatat cctgttttat aaattgattg gcgataatat ctttaccttc ttctataaaa
      481 aggtagcagt gttatttttt gaatgtttag tgttcataat tctaagtatc actattattt
      541 caattatata ttcggatttc tcacattact tactattaat aatcttattt tcactcgtta
      601 tcttacaata tatcttagtt gttggaacga ttagtatggt ttcacctaac attttaatca
      661 gcttaggtat cagcattgta tattggattg gttcagtcat cttagttgcc attaataaaa
      721 atatatttgg tatcgttgct ccatttgagg cgagcaatac gatgtatcgt gccgtagaaa
      781 aaatattgaa taacgagtca acatttatgt gtcctactga aattataaat atagtatcat
      841 tcttcgttct tctatttata gttaatacta ttgtcttatt actaagtaga aagagatggt
      901 tgaaaatcgg tatgtagtat aaaaaaggag cggtatacat tgccgcttct ttttattatg
      961 tatttttaca atctccacca atgacacgtc gtcattgcta cctcatacca aacaaaaaac
     1021 agtctcgcaa tcaagactgt tttccactca atatattcat ccattagcgt aatagattat
     1081 ttgacttctg tagctacaaa gattttacgt ttttcccaaa cgcctgtctt ttcattgtaa
     1141 tcatcacaag taattaatgt taattgttta tctttacctt tttgttcatc tagaactcct
     1201 acatctgtag gcttaacatc tcttatactt gtcattttat acttacgtgt ttcattacca
     1261 actttaaagt acaccatact accttttttg gctgctttaa gatttgtaaa ttgatagttc
     1321 ggacggtcaa tgaaagtgtg tcctgcaatt gaaatatttt gatcatctag tgattcattt
     1381 tcttctgcaa agcttacacc tctatttaat tgttcaggtg ttgctggtcc tggatatact
     1441 ggttctttaa tatcagcatc tggaatttca atatagcctg ccactttcga tttatctttc
     1501 ggaatttgag gtttagcttg ctgcttttta tctttactcg cctgttcttt tacattttta
     1561 tcatattgtt caatcttttc atctttatct ttatcgtgaa gataattatc gatatgtggt
     1621 ttagcaaaca aatatgctgc cactaggata agtaccacac cagcgattgt cattaatcga
     1681 tttgtccatt ttttcatacg ttaaggctcc ttttatacat ttcacaatgc ctgttaacat
     1741 ttcatatagt ataccattaa tttcaaaatg actcatagca attcatttta tactataaaa
     1801 tttacatgta tacttttacg ttagatttca ttacacatat ttgcattcaa ataacgaaac
     1861 gcttttaata attactaagg gggaattgat atgattagat acgctaaaaa agaggattta
     1921 aacgctatat tagcgatata caatgatgcc attatcaata ctacagctgt ttatacttat
     1981 gaaccacaaa ccatagacga acgtgtcgca tggtttgaaa cgaaacaacg taagcatgag
     2041 cctatctttg tatttgagga aaatggaagt gtcttagggt ttgcgacgtt cggttcattt
     2101 agaccttggc cagcatacct atatacaatc gaacattcta tttatgtcga tgcttcagct
     2161 agaggaaaag gtattgctag tcaattacta caccatttaa ttgtggaagc aaaagctaaa
     2221 ggttatcgtg cgctagttgc aggcattgat gcttccaaca aagcgagtat tcagttgcat
     2281 caaaagtttg cttttaagca tgccggcaca ctgaccaatg taggttttaa atttaataga
     2341 tggttagatt tagcatttta cgaattagat ttacaagact agtaatgttt gaatcacata
     2401 atataaacaa gacaaccatg ttaattccct taacataaca agccaacata taaaatttta
     2461 aacttctcag gggagtggga cagaaatgat aaagagccac taatgattta ttatgtagtg
     2521 gttctt
//
LOCUS       DAMQYT010000258         2504 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.265, whole genome shotgun sequence.
ACCESSION   DAMQYT010000258 DAMQYT010000000
VERSION     DAMQYT010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.265"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            321..1268
                     /gene="hlgC"
                     /locus_tag="PGA33_002597"
     CDS             321..1268
                     /gene="hlgC"
                     /locus_tag="PGA33_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HDG6474339.1"
                     /translation="MLKNKILTTTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKA
                     MRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSF
                     NYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKD
                     PRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTH
                     YGNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN"
     gene            1270..2247
                     /gene="hlgB"
                     /locus_tag="PGA33_002598"
     CDS             1270..2247
                     /gene="hlgB"
                     /locus_tag="PGA33_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HDG6474340.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(2299..>2504)
                     /locus_tag="PGA33_002599"
     CDS             complement(2299..>2504)
                     /locus_tag="PGA33_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDG6474341.1"
                     /translation="YLLKINVLSLVLASVLGSLVSRLLLSIILNLPVWVVLLNAIPGV
                     IFTLIVAIPLYLTLRKRMAVLLR"
BASE COUNT          993 a          392 c          389 g          730 t
ORIGIN      
        1 catcaaaaat cgtaaaaagc acaatttgta ttttacaaac attaattaaa aaagaaagca
       61 agacattcgt gcaatcggtt accttaaatt gtttacaact gtcaacaata ccaaggtttt
      121 attaactata tttctcacaa aattagcttt tagcattcca aacaaaaaag gttaaatcga
      181 acggaattat ggcattttta acttaattgt aaaaaaagtt gataatggtc aattgttaat
      241 gaacagttaa ttataataac gcccaaaata tattattatt taattaagtt aaataaaatt
      301 atagaaataa agtgaaactt atgcttaaaa ataaaatatt aactacaact ttatctgtga
      361 gcttacttgc ccctcttgcc aatccgttat tagaaaatgc taaagctgct aacgatactg
      421 aagacatcgg taaaggaagc gatatagaaa ttatcaaaag gacagaagat aaaacaagta
      481 ataaatgggg cgtgactcaa aatattcaat ttgattttgt aaaggataaa aaatataaca
      541 aagatgcttt gatattaaag atgcaaggat tcattagctc tagaacaaca tattacaact
      601 ataaaaaaac taatcatgtt aaagctatgc gatggccatt ccaatataat attggtttaa
      661 aaacaaatga taaatatgtt tctttaatta attatttacc taaaaataaa attgaatcta
      721 caaacgtgag tcagacatta ggatacaata tcggtggtaa tttccaatca gccccatcac
      781 tcggtggtaa tggatcattt aactattcta aatcgattag ctatacacaa caaaattatg
      841 taagtgaagt agaacaacaa aactcaaaaa gtgttttatg gggcgtcaaa gcgaattcat
      901 tcgccactga atcaggtcaa aaatcagcct ttgatagcga tttatttgta ggctacaaac
      961 ctcatagtaa agatcctaga gattatttcg ttccagacag tgagttacca cctcttgtac
     1021 aaagtggatt taacccttca tttatcgcca cagtatctca tgaaaaaggt tcaagcgata
     1081 caagcgaatt tgaaattact tacggaagaa acatggatgt cactcatgcc attaaaagat
     1141 caacgcatta tggcaacagt tatttagacg gacatagagt ccataatgca tttgtaaata
     1201 gaaactatac tgtgaaatac gaggtcaatt ggaagactca tgaaatcaag gtgaaaggac
     1261 agaattgata tgaaaatgaa taaattagtc aaatcatccg ttgctacatc tatggcatta
     1321 ttattacttt ctggtactgc taatgctgaa ggtaaaataa caccagtcag cgtaaaaaaa
     1381 gtcgatgaca aagttacttt atacaaaaca acagccacag cagattctga taaatttaaa
     1441 atttcacaga ttttaacatt taatttcatc aaagataaaa gttatgataa agatacttta
     1501 gtacttaaag ctactgggaa tattaactca ggctttgtga aacctaatcc taatgactat
     1561 gacttttcaa aattatattg gggagctaaa tacaatgtat ctataagctc acaatctaat
     1621 gattcagtaa acgtcgttga ttatgcacca aaaaatcaaa atgaagagtt tcaagttcaa
     1681 aatactttag gctatacatt tggtggtgac attagtatct ctaatggttt atctggtgga
     1741 cttaatggaa atacagcttt ttctgaaaca attaattata aacaagaaag ttacagaaca
     1801 acattaagtc gcaacacaaa ttataaaaat gttggctggg gagttgaagc acataaaatt
     1861 atgaataatg gttggggacc ttatggaaga gatagcttcc acccaacata tggtaatgaa
     1921 ctcttcttag ctggcagaca aagcagtgca tacgctggcc aaaacttcat agcgcaacac
     1981 caaatgccat tattatctag aagtaacttc aatccagaat ttttaagcgt actatcacac
     2041 agacaagatg gcgctaaaaa atctaaaatt acagtaactt atcaacgtga aatggattta
     2101 taccaaattc gttggaatgg cttctactgg gcaggcgcaa attataaaaa ctttaaaact
     2161 agaacattta aatcaacata tgaaattgat tgggaaaatc acaaagtgaa attgttagat
     2221 acaaaagaaa ctgaaaacaa taaatagcta gtaaaacacg gtcgccaaca gtaattgtga
     2281 cgaccgtgtt ttgatttatt atcttagtaa tactgccatt ctttttctca atgtgagata
     2341 taaaggaata gctacaatta aagtgaatat tacgcctgga atcgcgttta acaacactac
     2401 ccacacaggt aaatttaaaa taatagataa tagtagccta gataccaaac tgcctaatac
     2461 acttgctaaa actaatgata gtacatttat tttcaataaa taaa
//
LOCUS       DAMQYT010000259         2495 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMQYT010000259 DAMQYT010000000
VERSION     DAMQYT010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.266"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            107..406
                     /locus_tag="PGA33_002600"
     CDS             107..406
                     /locus_tag="PGA33_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HDG6474342.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRTQEKIYDK
                     LDRNFEELRRDKEEDEKNKEKNAKNIRDIKMWILGLIGTILSTFVIALLKTIFGI"
     gene            542..844
                     /locus_tag="PGA33_002601"
     CDS             542..844
                     /locus_tag="PGA33_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDG6474343.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDEESVSSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAESKYRKATGQAPIKEVMTPTNMNDTNDLG"
     gene            855..2309
                     /locus_tag="PGA33_002602"
     CDS             855..2309
                     /locus_tag="PGA33_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDG6474344.1"
                     /translation="MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMLATGE
                     RLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESA
                     NLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNK
                     AKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGES
                     ASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSE
                     LGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGVVASEVKAPVKNEKNPPVPAGYT
                     LDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDIAGNRISSFGKFSAV"
BASE COUNT          928 a          327 c          512 g          728 t
ORIGIN      
        1 agataaagag gaatatgcaa ttattacagg agaaaagtat ccagaaaatc tagagtcata
       61 ggttataatc ttatggcttt ttaatttgaa taaagtgggt ggtgtaatgt ttggatttac
      121 caaacgacac gaacaagatt ggcgtttaac gcgattagaa gaaaatgata agactatgtt
      181 tgaaaaattc gacagaatag aagacagtct gagaacgcaa gaaaaaattt atgacaagtt
      241 agatagaaat ttcgaagaac taaggcgtga caaagaagaa gatgaaaaaa ataaagagaa
      301 aaatgctaaa aatattagag acatcaagat gtggattcta ggattaatag ggacgattct
      361 aagtacattt gttatagcct tgttaaaaac tatttttggc atttaaagga ggtgatcacc
      421 atgcttaagg gaattttagg atatagcttt tggtcgtgtt tctggtttgg taagtgtaag
      481 taatatttaa gagtcagtgc ttcggcactg gctttttatt ttggataaaa ggagcaaaca
      541 aatggatgca aaagtaataa caagatacat cgtattgatc ttagcattag taaatcaatt
      601 cttagcgaat aaaggtataa gtccgatacc agtagatgaa gaaagtgttt catcgattat
      661 cttaacagtt gttgctttat atactacata taaagataat ccaacatctc aagaagggaa
      721 atgggcgaat caaaaattaa agaaatataa agctgaaagt aaatatagaa aagcaacagg
      781 gcaagcgcca attaaagaag taatgacacc tacgaatatg aacgacacaa atgatttagg
      841 gtaggtgttg accaatgttg ataacaaaaa accaagcaga aaaatggttt gataattcat
      901 tagggaagca gttcaatcct gatttgtttt atggatttca gtgttatgat tacgccaata
      961 tgttctttat gttagcgaca ggcgaaaggc tgcaaggttt atatgcttat aatatcccgt
     1021 ttgataataa agcaaagatt gaaaaatatg gtcaaataat taaaaactat gacagctttt
     1081 taccgcaaaa gttggatatt gtcgttttcc cgtcaaagta tggtggcgga gctggacacg
     1141 ttgaaattgt tgagagcgca aatttaaaca ctttcacatc atttggtcaa aactggaacg
     1201 gtaaaggttg gactaatggc gttgcgcaac ctggttgggg tcctgaaact gtgacaagac
     1261 atgttcatta ttatgacaat ccaatgtatt ttattaggtt aaacttccct aacaacttaa
     1321 gcgttggcaa taaagctaaa ggtattatta agcaagcgac tacaaaaaaa gaggcagtaa
     1381 ttaaacctaa aaaaattatg cttgtagccg gtcatggtta taacgatcct ggagcagtag
     1441 gaaacggaac aaacgaacgc gattttatac gtaaatatat aacgcctaat atcgctaagt
     1501 atttaagaca tgcaggacat gaagttgcat tatacggtgg ctcaagtcaa tcacaagata
     1561 tgtatcaaga tactgcatac ggtgttaatg taggcaataa aaaagattat ggcttatatt
     1621 gggttaaatc acaggggtat gacattgttc tagaaataca tttagacgca gcaggagaaa
     1681 gcgcaagtgg tgggcatgtt attatctcaa gtcaattcaa tgcagatact attgataaaa
     1741 gtatacaaga tgttattaaa aataatttag gacaaataag aggtgtaaca cctcgtaacg
     1801 atttactaaa tgttaatgta tcagcagaaa taaatatcaa ttatcgctta tctgaattag
     1861 gttttattac taataaaaat gatatggatt ggattaagaa aaattacgac ttgtactcta
     1921 aactaatagc tggtgcgatt catggtaagc caattggtgg agtggtagct agtgaggtca
     1981 aagcgccagt taaaaacgaa aagaatccgc cagtgccagc aggttataca ctcgataaga
     2041 ataatgtccc ttataaaaaa gaacaaggca attacacagt agctaatgtt aaaggtaata
     2101 atgtaagaga cggttattca actaattcaa gaattacagg ggtattaccc aacaacacaa
     2161 caattacgta tgacggtgca tattgtatta atggttatag atggattact tatattgcta
     2221 atagtggaca acgtcgctat attgcgaccg gagaggtaga catagcaggc aaccgaataa
     2281 gcagttttgg taagtttagt gcagtttgat aattgtatat gatgaatctt aggcaggtac
     2341 ttcggtactt gcctattatt taaaattaat aaacagttaa tttttacatg aatatattaa
     2401 attttaaaaa aacaaacgtt tttagtatat aaattatttt gtgttcgtat tgtgtgctat
     2461 gattaaaaag ttgttatggt caactatatc gtggt
//
LOCUS       DAMQYT010000260         2480 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMQYT010000260 DAMQYT010000000
VERSION     DAMQYT010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.267"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(327..653)
                     /locus_tag="PGA33_002603"
     CDS             complement(327..653)
                     /locus_tag="PGA33_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDG6474345.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSANVEMFVEYV
                     KQHMIV"
     gene            complement(717..1460)
                     /locus_tag="PGA33_002604"
     CDS             complement(717..1460)
                     /locus_tag="PGA33_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HDG6474346.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDSLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTIDFSCDYVSYLLSSQELLRNLKCYLNMCQLSLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVNLNNK"
     gene            complement(1486..>2480)
                     /locus_tag="PGA33_002605"
     CDS             complement(1486..>2480)
                     /locus_tag="PGA33_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDG6474347.1"
                     /translation="LGVNPENLHIIVTIDTSNELSIEQITLLLQKIVNYNVNVVFRIK
                     HEYKELLTSAERGKIEKLVATIFNMTFESNRCKIAFLINDLNTTAIQKLKRLIDNYIG
                     VFELIYHLEPKELNTRYDKEIDHLIDYFILPLRQIEQVSIARSKIICDVDLINENNDL
                     KEKITMSDINKVSELLTNYTKFRGLIMHMSIISESSKEINLTRLSIFIFIINILNQLR
                     GIVIYQNDTMIVTKFKHEVQCVICLPAALLNENSDAIRLSCKGLQQFSHAKTKQIVLS
                     IDKDNHTSENGVLVNDIMQQHSYWKANFIERNIYDHSLMVSSNSIVHLKYHLEI"
BASE COUNT          849 a          404 c          270 g          957 t
ORIGIN      
        1 caaataagta taaaaattca atatttttat tactagaata tggttaaata catttatttc
       61 ttataataat ttgatgttta agatattttg ccaaattgaa aattattagt ctgaatattt
      121 tgtatcttaa ctgatatgta aacttttaaa attttaatct atatcaacgt gtttgatttg
      181 atatttttta tattaattgg atggcttgct cacaaaattt ataaaatata aaaaaagaag
      241 agctttaaat cataacgcat gccatttatc tttaaatgca atgagttatc attcaaactc
      301 ttcttatcaa cctacataca ttgacattat actatcatat gttgttttac atattcaaca
      361 aacatttcaa cgttagcact tttaatatca tgcattggga cattaccaat attaaatttg
      421 aacattatag tttgaccatc ataatctatc ttctctattt cattataact aatacttcta
      481 taataaaatt ggccattcat atctacattc ataattaatc tttcatttgt tgctataaat
      541 gccccttcaa attcattctc accttgaact tgatactcaa ttattcctag cactgacgga
      601 cccttttttt cagttggaaa taaatcattt ggattcacat tatctaaaat cataatatcc
      661 ctcccactta aaactataaa ctattcttca taggtatatg aaatacaagt gattaactat
      721 ttattattaa gattaacttg tattcccttt tcaagataat tataacatgc ttgaataagc
      781 atagtgactt tagaaaactt ttctcgcttg attgttacaa taacacgcct ttcatctaat
      841 tcacatctct cttttttaac taatcccttc aaaattaaac actttaaaat cgggctaacc
      901 gcaaatatcg ggtgatggaa ttcaccctgt aattctttaa ccgtcaattg acctttatgc
      961 agatttaata ttcctaaaac gtataactct tctaatgata attgacacat atttaaataa
     1021 cactttagat tccgcaataa ttcttgtgac gacaataaat acgatacata gtcacaacta
     1081 aaatcaattg tatattgacc aaaataacgt tcaatatagt cactaagctc attattcata
     1141 tattctattt tatctatatg cattttatta atcgaaatag tcaatttacg ttgatccctt
     1201 tgatctcttc ctttcaatag ccattgatga ttgtttaaat aacttaacat ttgaagaagg
     1261 gccatcttag attggatttc tttcattttt aataaactat ctattgaagc gctattggaa
     1321 tcagaattat catatttaac taatagattt aatagtttca cttgagtcaa agtgagatga
     1381 aatgaatatt tcaacatatc attaatgact ttcaacatta gtatgtgtgc agttaaacgc
     1441 ttaactttaa atttagacat ctttaaaacc tctcttaaac catgcctata tctcaagatg
     1501 atatttcaaa tgaacaatac tattgcttga gaccattaat gaatgatcat aaatatttct
     1561 ttctataaaa ttagctttcc aataactgtg ttgttgcata atatcattca caagtacacc
     1621 attttcggaa gtatgattat ctttatctat acttaaaaca atttgtttag ttttagcatg
     1681 gctaaattgt tgaagaccct tacacgataa acgtatagcg tctgaattct catttaacaa
     1741 tgcggctggc aaacaaatga cacattgtac ttcatgtttg aacttcgtta caatcatcgt
     1801 gtcattttga taaataacaa tccctcgtaa ttgattaagt atatttatga taaaaatgaa
     1861 tattgataat ctagttaaat ttatttcttt gctcgattca cttataattg acatgtgcat
     1921 aataagtcct cgaaattttg tataatttgt caaaagttca gaaaccttat taatatcaga
     1981 cattgtgatt ttttctttta agtcattatt ctcattaatc aaatctacat cacatataat
     2041 tttagaacgt gcaatagaca cctgttctat ttgtcttagc ggtaagataa aatagtctat
     2101 tagatgatca atttctttat catagcgcgt attcaactct tttggttcca gatgatatat
     2161 taattcaaat actcctatat aattatctat taaacgtttc agtttttgaa ttgcagtagt
     2221 atttaagtcg ttaattaaaa atgcaatctt acatctatta ctctcaaaag tcatattaaa
     2281 aattgtagct actagctttt ctatcttgcc tctttcagca ctcgttaaca attccttata
     2341 ttcatgttta atacgaaaca ctacatttac attgtaattc actatctttt gaagtaataa
     2401 agtgatttgt tcaatcgata gctcattgct tgtgtcgatt gtaacaatta tatgcaagtt
     2461 ttcaggatta acacctaatc
//
LOCUS       DAMQYT010000261         2452 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMQYT010000261 DAMQYT010000000
VERSION     DAMQYT010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.268"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..1004
                     /locus_tag="PGA33_002606"
     CDS             <1..1004
                     /locus_tag="PGA33_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDG6474348.1"
                     /translation="VTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKETISAE
                     NKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYNNFIMI
                     SYMKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQNNKEYD
                     VFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQNFDLV
                     NAERKQHQLSTLKYSKQNSETARKHSKDMANNHYFDHTNLKGQSPFDRLKKDGITFNS
                     AGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTENYTG"
     gene            complement(1384..2007)
                     /gene="lexA"
                     /locus_tag="PGA33_002607"
     CDS             complement(1384..2007)
                     /gene="lexA"
                     /locus_tag="PGA33_002607"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDG6474349.1"
                     /translation="MRELTKRQSEIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            2150..2383
                     /locus_tag="PGA33_002608"
     CDS             2150..2383
                     /locus_tag="PGA33_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474350.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANISAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
BASE COUNT          909 a          363 c          385 g          795 t
ORIGIN      
        1 tagtaactgt tgttgcgttt ttatcaatta ttatcattgc gccaataaca gaatttaaac
       61 cattcattca tttacaaaat gaagtaagac aatatattga cattcacatc aataaagaaa
      121 caatttctgc ggaaaataaa ttggatacac cgaagaaaca acaatttgcc tttaataata
      181 tacaaatgaa catgtcgaaa tcagatgttg agaaaacatt aaataaacca aaaagagtga
      241 catttaatga atatggtacg aagtggtata cgtattatga tgacgattac aataatttta
      301 taatgataag ttacatgaaa gataaagtta atgcgttata tacaaatcaa aatataatca
      361 cttcaaaatc aaaaattaaa tataatacac ctaaatcggt tgtaaggcaa agattaggcg
      421 aaccagaaac agagattgtt aaaggtagag tgcgttacga acaaaataat aaagaatatg
      481 atgttttcca taaaaatcac atttatacga cggtatttta tgataagcat cgacgtaata
      541 atgtaacagc tgttttacaa gtaagtgatg ctatggaaaa tagattaaaa gaacaatatg
      601 gagcaccatc gaaatcgctt gcagatagtt ttgaactaca aaattttgat ttagttaatg
      661 ctgaaagaaa acaacatcaa ttatctacat tgaagtattc taaacagaat tctgaaactg
      721 cacgtaagca tagtaaagat atggccaaca atcattattt tgatcataca aatttaaaag
      781 gtcaatcacc atttgatcga ttgaaaaaag atggtattac atttaactca gccggagaga
      841 atttagcata tggtcaagtt agtagtatct atgcacatca aggattaatg aattctattg
      901 gtcacagaaa aaatatttta aatgatacgt ttaaaatatt aggtgttggt gttgatttta
      961 atgatgaaaa acaacctttt tggacagaaa attatactgg ttaatgtttt tggcatgaga
     1021 ttaaagggta atgtttgtct gtaaggaagt agataaacat gatgacaaca gataaaccaa
     1081 aagatgcgga tatacttgaa cgcgtcaaag atatacttaa taaaaaagaa agaaaaaaat
     1141 aataatggct cgctcctgta aattattacg ggggcgagtt ttaatttgtg taacattttg
     1201 cagaaatctg taattcaaaa tttatattaa agaatgagat tatctaacaa ttcgcaattt
     1261 ctgtttgaac tgaatttaga ttagaataaa taaaacctct tgaaaacgta actttttcaa
     1321 gaggtccaaa atttataaaa atgagggctt aacaattacg ataatatata acggtttaaa
     1381 atattacatt tcgcggtaca aaccaattac tttcccaatt acagcaacat tgtctaggta
     1441 aattggctcc attgtactat tttcaggttg taatcgataa cgattttttt ctttatagaa
     1501 gcgtttgaca gttgcttcat cttcctcagt catagcaaca ataatgtctc cattttctgc
     1561 tatggtttga ctgcgaacaa ttactttgtc tccgtctaat ataccagcct caatcatact
     1621 gtcgcctacg acgtttaata tgaatatgtc gctattgtgt gtcgatgtta agtgttcagg
     1681 taatggaaaa tattcttcaa tattttctac tgcggtaata ggaacacctg ctgtgacttt
     1741 accaataact ggcacatgaa tcgtttcttc catattaata ttatcatttg tttgatcact
     1801 tacaatttct atagcacgtg gtttcgttgg atctcttctt atatagcctt tttcttcaag
     1861 acgtgaaagg tgaccatgaa cagttgaact ggatgctaag ccaactgctt caccaatttc
     1921 gcgaacacta ggcggataac ctttcgtttg aacaacttgt ttaatatagt tatatatttc
     1981 gctttgtcgt tttgttaatt ctctcatata taggcactcc ctaaattaat ttaaatacag
     2041 tatatcatta atttgaaacc aaaacaaaca tttgttcgtt aaaatattga cacagaacat
     2101 aagttctgat aaattactaa tacaaacaaa tgttctagga gtgaaaatga tgttttacaa
     2161 taaatataaa aacgtatcaa catatatcat catattttta gtttcaagtg cagcctttgc
     2221 aatattcttg ttaagtgcga acattagtgc tcactcggaa caagtgtacg aaatgactga
     2281 ccatcaaatt aagaacaata cgataaataa agcatacgaa cataaagacc ctacaaacaa
     2341 tagcgaacaa agagatggga aagtgttcgc tttaataaat tgatacattg tcacaacgtt
     2401 attttgccta tttttgcgaa atagcgtttt ttattacttt tttgctgatc tt
//
LOCUS       DAMQYT010000262         2387 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMQYT010000262 DAMQYT010000000
VERSION     DAMQYT010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.269"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            970..1164
                     /locus_tag="PGA33_002609"
     CDS             970..1164
                     /locus_tag="PGA33_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDG6474351.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(1397..2248)
                     /locus_tag="PGA33_002610"
     CDS             complement(1397..2248)
                     /locus_tag="PGA33_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDG6474352.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTSLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
BASE COUNT          890 a          353 c          367 g          777 t
ORIGIN      
        1 aaatcaatta aataattatt aacaaagata taaaatatta ttattacaat atattaattt
       61 tgagctggaa atattcgtta tttatgctat aatcatttta gacacagcaa tgtgttcaaa
      121 ttttcatcta ttcgtaagtt agccttcggg ctgacttttt atttccatta ttcacatgtt
      181 aatcttgttg ttatttaggc aggtacttcg gtacttgtct atttttttat gttataatta
      241 catgtgtata tagtaggagt gaactatata gcccggcaga ggccatatat ctggctgttg
      301 gtctcacagg agacatcttc cttgtcatca ctcatataca tatatcttga taacatagag
      361 ttgttatagt cgctacgcca ctcatactag ttactgggtg gttgttttta tgttatatta
      421 taaatgatca aaccacacca cctattaatt taggagtgtg gttgttttca agggcaaaaa
      481 aagggcataa ttttgaaaca aagggcaagg gtatgaataa taaattatct tgaaaatttt
      541 ataaatgcat atataacaat gttttgattg cccttgaact tccttgataa tctggctaat
      601 tattcctgct aaataaatca tattaatggc tcagtaacgt gatgttgctg ggctttttaa
      661 tttaaacagg tatttatatg atatttagga atgagatgct attacggata aagtaaaatc
      721 ctatatgcat gcgtgctact catttattct tatttttaat ttcacatggc tagtgactaa
      781 tacatcataa atcattagtg attctttatt atttcagtcc cactccctca agttaatgat
      841 gaatctattt cgctagttat aaaatgacgt caaatcaata tcaaaaatta tttaagtaaa
      901 atgtttagat aatttttcag tgggtaagta ttatatataa catttaatta ttccgaggag
      961 gcatttatta tggcagacga aagtaaattt gaacaagcaa aaggtaatgt taaagaaaca
     1021 gtaggtaatg ttactgataa taaaaattta gaaaacgaag gtaaagaaga taaagcttct
     1081 ggtaaagcga aagaattcgt tgaaaatgca aaagaaaaag caactgattt tattgataaa
     1141 gtaaaaggta acaaaggcga gtaattttac tttttattcc taatacactg cacatgccac
     1201 ttagtcggta taaccatcgg ctaggtggtt tttgtattaa aaaagtggat accaaaattt
     1261 attaataatt attttaatgt tagaaaaaaa ctaaataaaa actcgctaat gatatccaat
     1321 aatatgtata caaaacgaga catatattgc atatgattaa cgagatactg aaaatatttt
     1381 atcaccccta aaatgattat tcattttcag cggtaattcg acctaaagtc aaacttacaa
     1441 taaaaccgat gataaatact actaatgaaa cgaaccacat cacgatatta gttggtaaac
     1501 ctggaaatac tgcaaagagg gagccaacaa caaaaccaat gattaatgca aaagtcatta
     1561 gtttatgatg tgttaggaaa tactggataa ttttgcttga aataatgaat ccagcaagca
     1621 cgccaaatcc gactgcaagt aatataggaa gacctgcaaa gttaagttta acaacttcag
     1681 atattgctag catgaccgta ccatagacgc caaatactaa taacataaat gaccctgaaa
     1741 tacctgggag taacatagca ctagatgcac acatacctgc aataaaatat ttaataataa
     1801 gactagttga tagagtaagt gtttctccag catgtttatc accattattc attaatgtaa
     1861 taacaattaa gatagcgata ccagctataa ccatcatgta atgtttagtt gtaaatgacg
     1921 ttttatagtt agaaattttc aataaatatg gaacgatacc aatgattaat ccaccaaaga
     1981 aaaacatagt tggaatatgg tgttggctta ataaataatt aaaaagatta cttagtgatc
     2041 ccattgccag taacattcca attataatgg ggattaaaaa tgtaaaactt ggccaaaaac
     2101 gtcgtgagaa tatgccgcta attgaagcga taaattgatt gtaaatacct aacaataatg
     2161 cgatagtccc accgctaaca ccaggtacca agtcactcgt tcccatagca aaacctttta
     2221 gaatattaat ccatttaaat tgttgcatga ataactcctt tcaaacgatt ggaataaaat
     2281 cataaatagc atcataccat attacaaatg tcctagtgaa atgataacat attttaaatt
     2341 cataaaatcc attgagaaat tatgtgcact tattatcatt tatattt
//
LOCUS       DAMQYT010000263         2368 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMQYT010000263 DAMQYT010000000
VERSION     DAMQYT010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.270"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            84..500
                     /locus_tag="PGA33_002611"
     CDS             84..500
                     /locus_tag="PGA33_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDG6474353.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     VRDLLQYKIDNYDNHIAKINASDNYDDNVERLWERK"
     gene            570..1418
                     /locus_tag="PGA33_002612"
     CDS             570..1418
                     /locus_tag="PGA33_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDG6474354.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            1638..1763
                     /locus_tag="PGA33_002613"
     CDS             1638..1763
                     /locus_tag="PGA33_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474355.1"
                     /translation="MRYIHFSIITLITGIIMHITMYFVPFESRKMPLFMTIIFTI"
     gene            2025..2368
                     /locus_tag="PGA33_002614"
                     /pseudo
     CDS             2025..2368
                     /locus_tag="PGA33_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          937 a          292 c          414 g          725 t
ORIGIN      
        1 aataagaagt attcaaacga agatgacaat atataaagca acattacttt agacgaccaa
       61 acataactga aaggatagtc gcaatgaaaa ctaaagaagt cgtagcgctc atgaatatat
      121 ctcaagacac tttaagatat tatgaaaagg ttggtgtgat tccaccagtt aatcgagatg
      181 aaaatggata tagaatatat aatgatagtg atttaaattg gatttattta gtgaaaaatt
      241 tgcgaaatgc aggcgtcagt attgaatcgc ttatcgaatt ttgcaggtta gcgcagcttc
      301 ctaaaaatga aaatattcaa gcacagcaaa agcaaatttt aaataagcaa ctcgaagaat
      361 taaatgaaaa cttaaagaca attcatgatg tgagagattt actacaatat aaaattgata
      421 attatgataa tcatattgct aaaatcaatg ctagtgataa ttatgatgac aatgttgaac
      481 gtctttggga gagaaagtaa acctatggct tgctatagag ttcgctccat ggtttatagt
      541 ggacatacaa gataaaggag gttcatttta tgaatcatat tgaaataagt aaagatgtaa
      601 agataccagt attaggtttc ggagtatttc aaattcctca agaacaaaca gcagaagcgg
      661 ttaaagaagc aattaaagcg ggttatagac atattgatac agcacaaagt tatttaaatg
      721 aaactgaagt aggtcaaggt attgaagctt caggtattga tagaagtgaa ctattcatta
      781 cgactaaagt atggattgaa aatgttaact atgaagatac tatcaaatca atcgaacgat
      841 cattacaacg attaaatttg gactatcttg atctagtgct tattcaccaa ccttacaatg
      901 acgtatatgg ctcatggcgt gcattagaag agttaaaaga aaatggtaaa attaaagcaa
      961 tcggtgtatc taacttcggc gttgatcgaa ttgtagattt aggtattcac aatcaaattc
     1021 aaccacaagt gaaccaaata gaaattaatc cattccatca acaagaagaa caagttgcag
     1081 cacttcaaca agaaaatgtt gtggtagaag cttgggcacc atttgcagaa ggtaaaaatc
     1141 aattattcca aaatcaattg ttgcaggcca ttgcagataa atacaacaaa tcaattgcac
     1201 aagttatttt acgctggtta gtagaacgtg acattgttgt actcgcaaaa tcagtgaatc
     1261 cagaacgtat ggctcaaaac ttagatatat ttgattttga acttacagaa gaagataaac
     1321 aacaaattgc gacattagaa gaaagtaata gccaattttt ctcacatgct gatccagaga
     1381 tgattaaagc acttacaagc agagaattag atgtttaatc ataatataac aaagtaaatc
     1441 atttacatta cactaacctg aaacttggat gttaaagtct atgtttcagg ttatttttta
     1501 ctcttaaaaa gtgaaaggag caagcaaata agttattata tatttttata ttatgtaata
     1561 acgtaactaa ggttataatt agataattaa tatgtcttgg aattaattat ttaataagtt
     1621 gtatagcagg tggcgatatg agatatatac acttttctat tattacatta attacaggta
     1681 tcattatgca tattacaatg tactttgtac catttgaatc tcggaaaatg ccactattta
     1741 tgacgataat ttttacaatc taaaagcagt aaagactcat aatataacca aaaacattaa
     1801 taaaagtttg ttaaatgata aggggatgag tattggtgaa tttactcaaa aggtttaagg
     1861 gaaaaatcct acctggagag attcaaaaac taaatggact atttccaaaa ataaagggca
     1921 atcacatggt ggttcttatt ggaagttaat aaataacaaa ggaaaaagaa tagcttcttt
     1981 aactaaagaa ggaaaaatct taagggaata aggtgttgta aattatgtgc tttgatataa
     2041 ataatttgga tataaaaaag ttgaatttta gaaaggtaaa aaacgcaatt catttaagtt
     2101 taagtggcga taaataccag tttttggagg acggtaaaac tatcaataat acttattttt
     2161 agctgtatat gataatgcaa taaacatttt tgaagatttg ttttcgcctt ctgattctat
     2221 aaatttggtt catgtagtat atgtttatag tgaaccttat aagaaaacaa atgttataaa
     2281 aaaatttaca tcgttaacaa tagatgaatt gtattacttt aaagaatata aaaaggagaa
     2341 ggatgataat attttgtgcg aggagttt
//
LOCUS       DAMQYT010000264         2359 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMQYT010000264 DAMQYT010000000
VERSION     DAMQYT010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.271"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..788)
                     /locus_tag="PGA33_002615"
     CDS             complement(<1..788)
                     /locus_tag="PGA33_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDG6474356.1"
                     /translation="MKFKSLITTTLALGVLASTGANFNNNEASAAAKPLDKSSSSLHH
                     GYSKVHVPYAITVNGTSQNILSSLTFNKNQNISYKDLEDRVKSVLKSDRGISDIDLRL
                     SKQAKYTVYFKNGTKKVIDLKAGIYTADLINTSEIKAININVDTKKQVEDKKKDKANY
                     QVPYTITVNGTSQNILSNLTFNKNQNISYKDLEDKVKSVLESNRGITDVDLRLSKQAK
                     YTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIE"
     gene            1177..2169
                     /gene="sph"
                     /locus_tag="PGA33_002616"
     CDS             1177..2169
                     /gene="sph"
                     /locus_tag="PGA33_002616"
                     /EC_number="3.1.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
                     /protein_id="HDG6474357.1"
                     /translation="MVKKTKSNSLKKVATLALANLLLVGALTDNSAKAESKKDDTDLK
                     LVSHNVYMLSTVLYPNWGQYKRADLIGQSSYIKNNDVVIFNEAFDNGASDKLLSNVKK
                     EYPYQTPVLGRSQSGWDKTEGSYSSTVAEDGGVAIVSKYPIKEKIQHVFKSGCGFDND
                     SNKGFVYTKIEKNGKNVHVIGTHTQSEDSRCGAGHDRKIRAEQMKEISDFVKKKNIPK
                     DETVYIGGDLNVNKGTPEFKDMLKNLNVNDVLYAGHNSTWDPQSNSIAKYNYPNGKPE
                     HLDYIFTDKDHKQPKQLVNEVVTEKPKPWDVYAFPYYYVYNDFSDHYPIKAYSK"
BASE COUNT          820 a          358 c          326 g          855 t
ORIGIN      
        1 ttttcgatat gtttttttgt atctacgtta atattgatac ttttaatatc acttgaattt
       61 attaaattcg ctgtgtaaat acctgatttc aaatcgataa ctttcttcgt tccattttta
      121 aaattaactg tatatttcgc ttgcttcgat aatcttaaat caacatcggt aatacctcta
      181 tttgattcta aaactgattt aactttatcc tctaaatctt tgtaactaat attttgattc
      241 ttattaaatg ttaagtttga taaaatattt tggcttgtac cgttcacagt gattgtgtat
      301 ggaacttggt aatttgcttt atctttcttt ttatcttcaa cttgcttttt agtatctacg
      361 ttaatattaa tagctttaat ttcacttgta ttaattaaat cagctgtgta aatacctgct
      421 ttcaaatcga taactttctt tgttccattt ttaaagtaaa cagtatattt cgcttgcttc
      481 gatagtctta aatctatatc actaatacct ctgtctgatt ttaaaactga tttaactcta
      541 tcctctaaat ctttataact aatattttga ttcttattaa atgttaagct tgataaaata
      601 ttttggcttg taccgttcac agtgattgca tatggaacat ggactttaga atatccatgg
      661 tgtaacgaac ttgatgattt atctaatggc ttagctgcgg cagacgcttc attattatta
      721 aagtttgcac ctgttgatgc taaaacacct aatgctaaag ttgttgtaat caatgactta
      781 aatttcataa attatctctc cttttttgtg taattcgtat ttgcaactta attatagcca
      841 gactttctct attttttgaa ttaactgaat attaataata aattatcttt aacaataatt
      901 ttttaacact gttaaaagtt cttttaattt tgattaacta attaatttac aatacctaaa
      961 atgttgtttg gttttgttta taccaagctt caaacttaaa tgtcataaca acattcattt
     1021 cttaattccg attagatttg tcgattatat ttacagcatc tttatactca aaaaacattt
     1081 acttaaaaat ataaattcga tttaataatt aatttaaatt tagttaatca attttgcatc
     1141 tattttgttg taagctatat aaaaggagtg ataatgatgg tgaaaaaaac aaaatccaat
     1201 tcactaaaaa aagttgcaac acttgcatta gcaaatttat tattagttgg tgcacttact
     1261 gacaatagtg ccaaagccga atctaagaaa gatgatactg atttgaagtt agttagtcat
     1321 aacgtttata tgttatcgac cgttttgtat ccaaactggg ggcaatataa acgcgctgat
     1381 ttaatcggac aatcttctta tattaaaaat aatgatgtcg taatattcaa tgaagcattt
     1441 gataatggtg catcagacaa attattaagt aatgtgaaaa aagaatatcc ttatcaaaca
     1501 cctgtactcg gccgttctca atcagggtgg gacaaaactg aaggtagcta ctcatcaact
     1561 gttgcagaag atggtggcgt agcgattgta agtaaatatc ctattaaaga aaaaatccag
     1621 catgttttca aaagcggttg tggattcgat aatgatagca acaaaggctt tgtttataca
     1681 aaaatagaga aaaatggtaa gaacgttcac gttatcggta cacatacaca atctgaagat
     1741 tcacgttgtg gtgctggaca tgatcgaaaa attagagctg aacaaatgaa agaaatcagt
     1801 gactttgtta aaaagaaaaa tatccctaaa gatgaaacgg tatatatagg tggcgacctt
     1861 aatgtcaata aaggcactcc agagttcaaa gatatgctta aaaacttgaa tgtaaatgat
     1921 gttctatatg caggtcataa tagcacatgg gaccctcaat caaattcaat tgcgaaatat
     1981 aattacccta atggtaaacc agaacattta gactatatat ttacagataa agatcataaa
     2041 caaccaaaac aattagtcaa tgaagttgtg actgaaaaac ctaagccatg ggatgtatat
     2101 gcgttcccat attactacgt ttacaatgat ttttcagatc attacccaat caaagcctat
     2161 agtaaatagt gctcaactaa ctaataactt gcttcgttct aaaaggacga agcgagttat
     2221 attgttaaaa tttgaattga cttacatttt aataaaatca tcttaacaac tttaattttt
     2281 cattaataca agtctttact ctacactcaa acaagattca tacactgcac gtcataataa
     2341 atctatctat tcaaatata
//
LOCUS       DAMQYT010000265         2337 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.272, whole genome shotgun sequence.
ACCESSION   DAMQYT010000265 DAMQYT010000000
VERSION     DAMQYT010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.272"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            199..507
                     /gene="spn"
                     /locus_tag="PGA33_002617"
     CDS             199..507
                     /gene="spn"
                     /locus_tag="PGA33_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HDG6474358.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
     gene            complement(610..2118)
                     /locus_tag="PGA33_002618"
     CDS             complement(610..2118)
                     /locus_tag="PGA33_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HDG6474359.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASENQNALISNIN
                     VDNQEKQNNVNQAVQPQNNTNETSKVPANFVKLNDIKPGDTSIQGTTLPNQFILLTID
                     KKDVSSVEDSDSSFVMSDKDGNFKYDLNGRKIVHNQEIEVSSSDPYLGDDEEDEEVEE
                     TSTEEVGAEEESTEAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKG
                     HTSVKGKVALSINNKFINFETNANGGPNKEEAKSGSEGIWMPIDDKGYFNFDFKTKRF
                     DDLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLK
                     DVKEDLHVDEIYGSLYHTEKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEGQEFPD
                     LQVDEKGEFSFDVDHAGFRLQNGETLNFTVVDPITGELLSGNFVSKNIDIYESPEEKA
                     DREFDERMENTPAYHKLHGDKIVGYDTNGFPITWFYPLGEKKVERKAPKLEK"
     gene            complement(2140..>2337)
                     /locus_tag="PGA33_002619"
     CDS             complement(2140..>2337)
                     /locus_tag="PGA33_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HDG6474360.1"
                     /translation="ELDFKLRKHLIDKHNLYKTEPKDSKIRITMKDGGFYTFELNKKL
                     QTHRMGDVIDGRNIEKIEVNL"
BASE COUNT          710 a          411 c          314 g          902 t
ORIGIN      
        1 ttcatttgat ttaaaccctt tttgaaatca aggttttatt aggaaaaatc ctcaagcatc
       61 gtattaaaga ttaattgcat ttaaacaata tttgtcactt taactattta ttaaaaatta
      121 ttaaatcgac ttaataaacg taattttctt aatattcagt gttatgatgg agttacaatt
      181 tttagaggag tgatagatat gaaatttaaa aaggttttag tagctacggc aatggtaggt
      241 gttttagcaa ctggcgttgt tggatatggt aatcaagcag atgcgaaagt ttattctcaa
      301 aatggactcg tactacatga tgatgcaaac ttcttagaac atgaattaag ttatattgat
      361 gtgttattag ataagaatgc tgaccaagct acaaaagaca acttaagatc atacttcgcc
      421 gataaaggac tacattcaat caaagacatt atcaacaaag ctaaacaaga cggctttgat
      481 gtttcaaaat atgaacacgt aaaataaatt caataatgct ttgtaacaaa gcacaaaaaa
      541 cagctcaatg acgtagaagt cagtgattaa ctcactaatt tcattacatc attaagctgt
      601 tttatttaat tatttttcta attttggtgc cttacgttca actttctttt cacctaatgg
      661 ataaaaccag gtaatcggga atccgttagt atcgtagccg acaattttat caccatgtaa
      721 tttatgatat gcaggtgtgt tttccattct ttcatcaaac tcacgatctg ctttttcttc
      781 aggagattca taaatatcta tgtttttaga aacaaaattt ccacttaata attcacctgt
      841 aataggatca actactgtga agtttagtgt ttctccattt tgtaatctaa atccagcatg
      901 atctacatca aagctgaatt cacctttttc atcgacttgc aaatcaggga attcttgacc
      961 ttcacctagt tcagagtcta ccttcacaac tgcatttgcg aatttagtct tacctttaat
     1021 tactttagta ccttctttat caagaatacc tttacctttt tctgtatgat ataagcttcc
     1081 gtaaatttca tctacatgta aatcttcttt aacatctttc aatactttta ctttttccac
     1141 tttagtatgg tcatatttag tttctgcttt atcaatatct tctaaactcg ttaccttagt
     1201 tttgaaaatt aatgacttca atgcctcatc ttcgtcatca ggtgcaaatg ttaatgagat
     1261 ctcatcattt ttctttaact ctaaatcatc gaaacgtttc gttttgaagt caaaattaaa
     1321 gtatcctttg tcatcaatag gcatccagat tccttctgat ccagatttcg cttcttcttt
     1381 atttggacca ccattagcat ttgtctcaaa gttaataaat ttattattaa tagatagagc
     1441 aactttacct tttacagagg tatggccttt aataatacct gaaccttcag taataggttc
     1501 gataaaaact tggtgatgtc catctttttc ttttagctgt tctttcggta tttcatacgc
     1561 tttttcatat cgcggtgttg tatatgtagc tttagcttct gtactttctt cctcagcacc
     1621 aacttcttca gttgaagttt cttctacttc ttcatcttct tcatcgtcac ctaaataggg
     1681 atctgatgaa gacacttcaa tttcttgatt atgaacaatt ttgcgaccat ttaagtcata
     1741 cttaaaattc ccatctttat cagacataac aaagctgctg tcagaatctt caactgagct
     1801 cacatctttt ttatcaatag ttaatagtat aaattgattt ggtaaagttg ttccttgtat
     1861 agaagtatca cctggtttaa tatcattcaa tttgacaaaa ttagccggta cttttgatgt
     1921 ttcattagta ttattttgag gctgaacagc ttgatttaca ttattctgtt tttcctgatt
     1981 gtctacattt atattagaga ttaaagcgtt ttgattttca gatgcttctg cttgtccgtt
     2041 tgtcataata tataacattg taattgcaac agatactaaa ccgactttca ttttacgtaa
     2101 cttaaaattt tccctcatga tatgctccct cgaataattt tataaattca cttcaatttt
     2161 ttctatattt ctgccatcaa taacatcacc catacggtgt gtttgtaact ttttattcaa
     2221 ttcaaatgtg tagaacccac catctttcat agtaattcga attttactgt ctttaggttc
     2281 tgtcttataa aggttatgtt tatcaattaa atgctttctt aatttaaaat caagttc
//
LOCUS       DAMQYT010000266         2333 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMQYT010000266 DAMQYT010000000
VERSION     DAMQYT010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.273"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(95..562)
                     /locus_tag="PGA33_002620"
     CDS             complement(95..562)
                     /locus_tag="PGA33_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDG6474361.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(748..2298)
                     /gene="adcA"
                     /locus_tag="PGA33_002621"
     CDS             complement(748..2298)
                     /gene="adcA"
                     /locus_tag="PGA33_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HDG6474362.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHHGGYDPHVWLDPK
                     INQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISH
                     ESIGYLADCYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTET
                     IRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAES
                     KHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKD
                     LKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFK
                     LVDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQ
                     IKEEMLAH"
BASE COUNT          647 a          428 c          317 g          941 t
ORIGIN      
        1 cgttctacca tcgaatcatc cctcattttt ataatattac aagattatta taaaccgaat
       61 gaaacaaaaa agtttttaca tattttcaac aactttattt caataaagca tcaagttgat
      121 ctaaagtttg attcatacct tgttcaacac ccatgtttat gacttgttga gcggcttctt
      181 ttgttggaaa taccgatgtt gatgtcactg tcgttttcgt tgtattgctc ttggaaaatg
      241 acaaagtaat tttcatgctt ggcatttttg tatcttttgc accttgaggt gtcgcaaatg
      301 agtcaatata ttcaattaaa tatggacgct taaccgtttt atattctgct aatgtatagc
      361 tggtcattgt aggtgtttta atcgcataaa atgcatcacc gcctgaaaca gcattaaagc
      421 gaaacacttt ggtactagcg tcttttggat gaaaccattt ttcaaataat gcttttgtcg
      481 tataggcatc aaacaccttt tcaatcggtg cttctatcgt tcttgaaaaa ataattttat
      541 tatcttcaac tttaatcgtc atcttcccac tccctatctt ttatatttac tcatcttaac
      601 atgcattgag taacatatgt taccaaatca tttgttataa actatagtaa tattaatcat
      661 tccctctgtt gaaattgata actacgaaat aaaaaacaca ctctattcag ctattaacag
      721 ttgagtagag tgtgcaaacc tgttgaatta atgcgctaac atttcttctt tgatttggtc
      781 tttatttaat tttgaaggat aatatgttgg ccagttatcc atttctttta ataacgcgtc
      841 attatcatta cccataaaga tatggaagtg ttctgccttt ttaggtgcaa tgttgtgatc
      901 gctaaattgg atgaatttcg gtaagtcttt atcattacca tcgaccaatt taaacataaa
      961 tctcacgcca cggttacctt taggatattt caatgttttc ttaccattgt attcatattt
     1021 accagtgtgt ttcttaccat ctttagtaaa tgtaatttca tttcctttta tatcaatgtt
     1081 agtaatatca gttttatatc ctttgtcata ataagctttt aaatctttag cagatttctt
     1141 cggatcattt tcagctttat gttccatcac ttcatcaagc gtaccgtctt ttaagtaagg
     1201 gtaaacagat tgccattcac cagcataatc gcttaattca cggtctttaa cttgctcatc
     1261 tttaaaatac ccatcagaaa ttgctttgtc atgtttactt tcagctttgt cgtctttcac
     1321 tttaacacca ctgtctaaag ctttaccgat attttcaata ttagatttca ttaatgattg
     1381 atacgtaata ttatcttttt tctgttgttc tttatttaaa gactccatgt tgtagaattt
     1441 taaaggcttc gcatctgttt ctttacgaat tgtttcagtc actttattcg caacattatc
     1501 ttcgtaaaga atatatttag cattgctatc tctaatttct ttaacaattt tagttaattc
     1561 tttttgtgat ggatcttcag cattcatgtt ttgaatacct ttttgaacaa aaccataaca
     1621 atcagctaag tatccaattg attcatgtga aatgaatact gcattacctt gcttatcttt
     1681 tgtaacttgc ttcatatcgt tatcaatttt cttaagatcg tcgtttaatt ttttgtagtt
     1741 tttctcatag tcatctttat gttttggatc ttttttcact aattcatctt taatttcttt
     1801 agcgaaagtt tggttaattt taggatctaa ccatacgtgt ggatcatatc caccatgatg
     1861 atgatgatgt tcttctttct catgatcatg tccctcatgt tcatgctctt caccatgctc
     1921 gtgttgatca gttaaaagct ttgctttatc taatttatcc tctaaagaca gttttttatc
     1981 tttatctttg atagtagatg caactttctt agcaaccgga tctaaattat cccctgtata
     2041 cataaacaag tctgacttgc ttgcacttaa tatatctttt tgtgttggtt catagctatg
     2101 taaatctgtc cctgctggat agattgatga taccttcacg tgttttccac caatttgctc
     2161 tgcaaatgat tgcaatggat aaactgtcgt tttaattgtt accttgccat ctttatcttt
     2221 tccatcatca ttcccacatg cggctaatga taaagttacg gctggtacaa gaagtaacat
     2281 acctaatttc tttttcatag tcaccctcct aaatagtaat ctttacgttt taa
//
LOCUS       DAMQYT010000267         2326 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMQYT010000267 DAMQYT010000000
VERSION     DAMQYT010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.274"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..287
                     /gene="holA"
                     /locus_tag="PGA33_002622"
     CDS             <1..287
                     /gene="holA"
                     /locus_tag="PGA33_002622"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDG6474363.1"
                     /translation="TMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHP
                     YRVKLALGQVRHYQLDELLNIIDACAETDYKLKSSYMDKQLILELFILSL"
     gene            complement(332..583)
                     /locus_tag="PGA33_002623"
     CDS             complement(332..583)
                     /locus_tag="PGA33_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDG6474364.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            929..>2326
                     /gene="lepA"
                     /locus_tag="PGA33_002624"
     CDS             929..>2326
                     /gene="lepA"
                     /locus_tag="PGA33_002624"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDG6474365.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCVLRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFD"
BASE COUNT          820 a          308 c          437 g          761 t
ORIGIN      
        1 taactatgaa agaagaacca attaaattac ttgcactaat tacaagtaat taccgattat
       61 tttatcaatg taagattctg agtcaaaaag gatatagtgg acagcaaatt gctaaaacaa
      121 taggagtaca tccatacaga gtaaaattag cgttaggaca agtaagacat tatcaacttg
      181 atgaattatt gaatattata gatgcttgtg cggaaactga ttataaactt aaatcatcat
      241 atatggataa acagttaata ctggaattat tcattctatc tttataaaaa ataaaagttc
      301 aagcatgcgc ttgaactttt atttaaaaag attatttatt tgcagtcatt aattgtgatt
      361 taatacggtc agctttgttt gaatgtatta aattactttg agcagctttg tctactaact
      421 taactgctaa gcttactaat tcatttttat tatcagcgtt atttgaaaca gctgttttag
      481 cgtttttaac tgctgtacgc attgcactct tttgtgaaat gttgcgtgct tcagcttttt
      541 cagttgtttt tacacgttta attgcagatt tgatatttgc catttctgtc acctcctaaa
      601 agtgagttca tcttatcaaa attttatttg attacaacaa gaaatatttt aacaaaagtc
      661 taaccgttgt gcaatcattt atttgatggg gttagcttaa aattttgcag atttaataaa
      721 ttgtgttgga aacacaatca atacgtttta tagttataag tacgtaatta acatattaac
      781 atatcgcaag tatgtattta aataaagatt gttataattt caaagttcat ccaagattat
      841 ggcgtttgca tttacctatt aaaaacgtta ttatatcaaa gatgcgaaag ataatacggg
      901 tttattttat gaaagtgaga aggataaaat ggataatgag caacgcttaa aaagaagaga
      961 gaatataagg aatttctcga ttatagcaca tattgaccac ggaaaatcta cattggctga
     1021 tagaatttta gaaaatacca aatcagttga aacaagagat atgcaagatc agttactaga
     1081 ttcaatggat ttagaaagag aacgtggtat tacaatcaaa ttaaacgcag tacgtttaaa
     1141 gtacgaagct aaagatggaa atacttatac attccattta atcgatacgc ctggacacgt
     1201 cgattttaca tatgaagtgt cacgttcttt ggcagcttgt gagggcgcga ttttagtagt
     1261 agatgcggct caaggtatcg aagcacaaac attagcaaat gtttatttag cattagataa
     1321 tgagttagag ttattgcctg ttattaacaa aattgattta cctgctgcag aacctgaacg
     1381 cgtgaaacaa gaaattgaag atatgatagg tttagaccaa gacgatgttg ttttagcaag
     1441 tgctaaatct aacattggaa ttgaagagat actagagaaa atagttgaag ttgtgccagc
     1501 tccagatggt gacccagaag caccactaaa agcgttaata tttgattctg agtatgatcc
     1561 atatagaggg gtaatttcat cgataagaat tgtggacggt gttgttaaag ccggagataa
     1621 aattcgaatg atggccactg gtaaagagtt cgaagtaaca gaagttggaa ttaatacacc
     1681 taagcagctt ccagttgatg aattaacagt tggtgatgtt ggttatatta ttgcaagtat
     1741 taaaaatgtt gatgattcta gggttggtga caccatcaca ttagctagta gacctgcatc
     1801 agaaccattg caaggttata agaaaatgaa tccaatggta tattgcggac tgttcccaat
     1861 agataacaaa aattataatg atttaagaga agcattagaa aaattacaat tgaatgatgc
     1921 atcattagaa tttgagcctg aatcgtcaca agcattaggt tttggttata gaactggttt
     1981 cttaggtatg ttacacatgg aaataattca agaaagaatt gaaagagaat ttggtattga
     2041 attaattgca actgcaccat ctgtaattta tcaatgtgtt ttaagggacg gttcagaagt
     2101 gacggttgat aacccagcac aaatgccaga tcgtgataaa attgataaaa tatttgagcc
     2161 atatgttcgt gcaactatga tggttccaaa tgactatgtc ggtgcagtaa tggaattatg
     2221 tcaacgtaaa cgtggacaat ttataaatat ggactattta gatgatattc gtgtaaatat
     2281 tgtttatgaa ttacctttag ctgaagttgt atttgatttc ttcgat
//
LOCUS       DAMQYT010000268         2324 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMQYT010000268 DAMQYT010000000
VERSION     DAMQYT010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.275"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(134..865)
                     /locus_tag="PGA33_002625"
     CDS             complement(134..865)
                     /locus_tag="PGA33_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDG6474366.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            complement(867..2180)
                     /gene="murT"
                     /locus_tag="PGA33_002626"
     CDS             complement(867..2180)
                     /gene="murT"
                     /locus_tag="PGA33_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HDG6474367.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
BASE COUNT          700 a          458 c          294 g          872 t
ORIGIN      
        1 attcactaga acaaaaaaac acacatcaat ttggttgttt ccatccaaaa taatgtgtgt
       61 gtttatacat atatgattaa ttactaaact tcatcgttta tcaatattaa atccattgat
      121 gcgatgttca gagttaacga gatttcttct gtctatttgc tctgtctatt aatacttgtt
      181 tcgcttgtat ttccgcttca ttatctattt ctttaggctc aaacggaata cccttacgtt
      241 cacaagcttt ttctaacaga taatcagtga tttcgtaatt tttaggtaaa attggtccat
      301 gtaaataagt acctaataaa tttttataat gaatgccttc ttttttatct tcatcattat
      361 taccataacc aaaagtaaca tgaccaagtg taccgaaatc atgatatgtt ctaccaccgt
      421 gattttcaaa acctacaata gttccaaaag tatcactttc gataacaata tctcctgtta
      481 atcggtttgt ctttgattca gtataaaaat ctaaaatacc taacccttct aattctgtac
      541 catcaggcgt gatatatttt ttccctaaaa attgatagcc tccacaaatc gttaatcccg
      601 gcataccatc ttcaatcgct tctttaagtg gtgtcttaat tttacttaat tcttttgttg
      661 ctaatgcttg ttctctatca cttccaccac cgataaagaa aatatcacat tcatcaaagg
      721 taataccttc tgtttcattg atttctacga cattaacttt aatatttcgt tttttagcac
      781 gttgtcttaa agcaataata tttcctatat cactgtataa attcaattta tctgacataa
      841 aatgataaat agtcaattca tgcatattat gattgacctc cttcaaacga acggtttaat
      901 tgttcaagca taggcgctaa tgatgtatag tttggtattg caactgtgaa acctttataa
      961 tccatagtct ttgccgttgc tttataaata tcacgctcaa ctataattgg tacttcaacc
     1021 tctgctaact tcaatcgcaa ttgaagttct tctgctcgtg tacctgtcac gatgatagct
     1081 tcaatttgtt gcttagataa tttttcaaaa tctgcatcat aaatccatga agtatctcga
     1141 ccatctgcag cgttatcatt tagcgaaata acatacactt tttcgccttc taattgttca
     1201 ccaactgata aacttgcatt cattcctgca ggatttttag ctaaattgat catcgcttct
     1261 tttcgttctt ttttaaagta ctgcatacga ccattgtctg atgtatacgt ttcaaagcca
     1321 tttttaattg tttgttcatt taaccctagc tctcttaaaa cagtatatgc tgctaacgcg
     1381 ttataagcgt taaagtcacc tgcaattttc atatcatatt tttcatcatt gatatttaaa
     1441 tataaaaacg gtgccacatc aaaacttgat atttcatatt ttgcttgctc tcgtttgaaa
     1501 ccacactgac agtgataatg accaatttga ttataatgaa tataatcgta ttgcaataag
     1561 cgaccacagt ttggacaata tctactttca ttcatcgtac tttgttcaaa ttcatgggca
     1621 tgtgctttca taccatagta cacaatcgta tcacttgcga ttttcaaacg actcacaaat
     1681 ggatcatcag catttagcaa taatttgatg cctttattac taattgtctc tgcaatgtta
     1741 ttaaccataa tatcaatttc accgaagcga tccatttgat ctctaaagaa attagtaaat
     1801 accatcattg aaggtgtaac ttcttttaac acacgtggaa tcgaaccttc atcaatttcg
     1861 attaccgcaa ttttagtctt aggtgttgat tgcatgatga atgcagaagt tatacctgca
     1921 gccatattag caccttcatt attgtgtata atttgaatat tatttgcttt taaagtatgt
     1981 ccaattaagt ttgaagtcgt tgttttacca tttgttccac tgataaatac aatatcatca
     2041 acttgctctg ctaattttct taatatatct gtatccactt ttctagcgat ttgtccaggt
     2101 aaatctgttc ctcttttacc tactgctcta cttgctttac gcgccaattt cgctagatgg
     2161 attgccgtcc actgtctcat gtgtttcctc ctcaaatttc cactcgcatc attataacat
     2221 gacaaggcaa cttcaaaaaa gtttctcaat cacaaattga tacagtgttt ctaaattata
     2281 atcattctca tttagataat taaaaatgat atttattctc aatt
//
LOCUS       DAMQYT010000269         2301 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMQYT010000269 DAMQYT010000000
VERSION     DAMQYT010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.276"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..1678
                     /gene="rsp"
                     /locus_tag="PGA33_002627"
     CDS             <1..1678
                     /gene="rsp"
                     /locus_tag="PGA33_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HDG6474368.1"
                     /translation="LSNPVFIDCVNYIHDNIDAHLSLKDIAMHCNISESYCSNLFVRY
                     LSMNFKDYFTSIKLVNAINLLLSTKHSITTVSELAGFNSHTNFANQFKNYLHFSPKQF
                     RSLVSKITEPPQIHFQQDNVSQFTELISTIDLTAQLATNTTDIHIDDFNPKDRSQRAK
                     VFVRFSNFNELFQFIFNEYYDINFEHLPKPVVFIDDIHDIEISQTNYNLLNRCFEKLF
                     EKNIGLAIAIKSTQQFETMKQLILTFLQGNQDYKTSKKLVKFMLVFCSNSMTAEEIHL
                     CHLKIKNKNKEIKYSVTVDGFLETYSTVEQVYDVMQRLKFHYYFIDIENSKTATHLIT
                     KNQHYHQTDTHFEQYKKFILDSGISSTQFVYNNLSVSGFKYTNDGKNPIQLSDIVYHL
                     IALLRYGGGISYQLLDDHSNYISLYNKYGSPLPLMHLYKMFRPFVNEDIEITNNYVLS
                     RKDNNYHFLLFNKINDRYMSDVKQDFIFHNELPQDSLMIIKTLNHEHGSIQHLLPISD
                     QLVYIEKEILDELDKTNYPKTELAVQEETGRTFELKLNHDEVKYICFKPS"
     gene            1749..2171
                     /locus_tag="PGA33_002628"
     CDS             1749..2171
                     /locus_tag="PGA33_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDG6474369.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEIVTEQEVIDWLWETQD
                     KSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKIDL"
BASE COUNT          857 a          356 c          319 g          769 t
ORIGIN      
        1 tttaagcaat ccggtgttca tagactgtgt aaactatatt catgacaata ttgatgcgca
       61 cttgtcttta aaagatatag cgatgcattg caacatctct gaatcatatt gttctaatct
      121 ttttgttcga tatttgagta tgaattttaa agattatttt acaagtatta aacttgttaa
      181 cgccatcaat ttattacttt ctacaaaaca ttctattaca actgtttctg aattggccgg
      241 ttttaacagt catacaaatt ttgcaaatca atttaagaat tacttgcatt ttagtcctaa
      301 acagttccgt tcactcgttt ccaagattac tgaaccacca caaatacact tccaacaaga
      361 taacgtatca caattcactg agttaatttc aacaattgat ttaacagctc aactggctac
      421 aaatacaaca gatattcata tagatgattt taatccgaaa gatcgaagtc agcgtgccaa
      481 agtatttgtt cgatttagta atttcaatga actatttcaa tttattttca atgaatatta
      541 cgatattaac tttgagcact taccaaaacc tgtcgttttc attgacgata ttcacgatat
      601 tgagattagc caaacgaatt acaacctgtt aaatagatgt tttgaaaagc ttttcgaaaa
      661 aaatataggt ttagcaatcg ctattaaatc aacacaacaa ttcgaaacga tgaagcaatt
      721 aatactaaca tttttacaag gcaatcaaga ctataagaca agcaagaaat tagttaaatt
      781 tatgctcgta ttttgctcta attctatgac agcagaagaa attcatttat gtcatcttaa
      841 aattaaaaac aaaaataaag aaattaaata tagtgtgaca gtcgatggct ttttagaaac
      901 ttattcaacc gtggaacaag tttatgatgt catgcagcgt cttaaattcc attattactt
      961 tattgatatt gaaaattcaa aaactgcaac acatcttatc actaaaaacc aacactatca
     1021 tcaaactgac actcattttg aacagtataa gaaatttatt ttagattcag gtatatcatc
     1081 aacacaattt gtatataata acctgtctgt aagcggattt aaatatacta atgatggtaa
     1141 gaatccaatt caattatctg acatagtgta tcacttaatc gcattattac gatatggcgg
     1201 tggcattagc tatcaattat tagatgacca ttcaaattat atttccttgt acaacaaata
     1261 tggtagcccc ctaccattaa tgcatttata taaaatgttt agaccttttg ttaatgaaga
     1321 tattgaaatt acaaataatt atgtattgag tcgtaaagat aataactacc atttcttatt
     1381 attcaataaa attaatgatc gatatatgtc agacgtaaaa caagatttca ttttccataa
     1441 tgaattacct caagactctt tgatgattat taaaacattg aatcatgaac atggttcaat
     1501 tcaacatttg cttccaataa gcgatcaact tgtttatata gaaaaagaaa ttttagatga
     1561 attagacaaa acgaattacc ctaaaacgga gcttgcagtt caagaagaaa ctggtcgtac
     1621 atttgaactc aagttaaatc acgacgaggt taaatatatt tgctttaaac caagctaaat
     1681 actaacagtc ctcttgtgtt tagtttctta cgttaaaggc tatttatatc ataaggagat
     1741 gatttgtaat gagtaactca caagcaattc aagcaattga aaacgtgtta gcaacgtcaa
     1801 aagttggtgt attatcaact gcatataata ataaacctaa tagtagatat atggtctttt
     1861 ataatgatgg tcttacttta tatactaaaa cgaatatcca ttctgctaag gtcaaagaaa
     1921 ttaaagataa tccagcagca tatgttttgt taggctataa cgacacaact aatcgtagtt
     1981 tcgttgagat ggaagcgaca atcgaaatcg ttacagaaca agaagtgatt gattggctat
     2041 gggaaacaca agacaaaagc tttttcagtt caaaagaaga tccagagtta tgtgttttaa
     2101 gagtagttcc gcaatccatt aagctaatga atgataaatc attagataca cctatcaaaa
     2161 tcgatttata acacaaagtg tatataggaa ataactttta tgaattctag atataacaat
     2221 gttaaatact taaaataact cgctataatt aaagtgttta atatgtttac aattcaattt
     2281 gcaatgtatt gttttttaga c
//
LOCUS       DAMQYT010000270         2250 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMQYT010000270 DAMQYT010000000
VERSION     DAMQYT010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.277"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            115..888
                     /gene="lpl1"
                     /locus_tag="PGA33_002629"
     CDS             115..888
                     /gene="lpl1"
                     /locus_tag="PGA33_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl1"
                     /protein_id="HDG6474370.1"
                     /translation="MGYLKRFALYISVMILIFAIAGCGKGNETKEDSKEEQIKKSFAI
                     TLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWTIYTDFAKSNKPGELDDEGMVLNLDR
                     NTRTAKGHYFVTTFYRNGKLPDEKNYKIEMKNNKIILLDEVKDDKLKQKIENFKFFGQ
                     YANLKELRKYNNGDVSINENVPSYDVEYKMSNKDEIVKELRSRYNISTEKSPILKMHI
                     DGDLKGSSVGYRKLEIDFSKRENSKLSVIEFLSYKPAKK"
     gene            919..1719
                     /gene="lpl2"
                     /locus_tag="PGA33_002630"
     CDS             919..1719
                     /gene="lpl2"
                     /locus_tag="PGA33_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl2"
                     /protein_id="HDG6474371.1"
                     /translation="MRYLKKLAWFISVIILGIFIIGCDSSSDTGEKAKEDSKEEQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWMIYTDFAKSNKPGVLDNEGMIL
                     NLDRNTRTAKGYYFVDTIYDNHENSYSKNYRVEMKNNKIILLDKVEDQKLKERIENFK
                     FFGQYADFKSLKSYNNGDVSINSNVPSYDAKFKMSNKDENVKQLRSRYNIPTEKAPIL
                     KMHIDGDLKGSSVGYKKLEIDFSKEENSELSVVDSLNFQPAKKNKDDE"
     gene            1738..2250
                     /locus_tag="PGA33_002631"
                     /pseudo
     CDS             1738..2250
                     /locus_tag="PGA33_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557596.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
BASE COUNT          962 a          212 c          447 g          629 t
ORIGIN      
        1 taaattgatt gtatatgtat attttggtaa cgtaaaagag aaatatacaa aataattaat
       61 tatttatatg aaaagagaat ataaatgaag tataaaacag agagacgtga agcgatggga
      121 tatttaaaaa ggtttgcatt gtacataagc gttatgattt taatatttgc gatagcaggt
      181 tgtggcaaag gtaatgaaac aaaagaagat tcaaaggaag aacaaatcaa aaagagcttt
      241 gcgataacat tagatatgta tccaattaag aatctcgagg acttatatga caaagaagga
      301 tatcgagata gcgaatttaa aaaaggtgac aaagggatgt ggacgatata tacagatttc
      361 gccaaaagta ataaaccggg tgaattggat gatgaaggta tggttttaaa tctggatagg
      421 aatactagaa ccgctaaagg ccattatttt gttactacat tttaccggaa tggtaaacta
      481 ccagatgaaa agaattataa aattgaaatg aaaaataata agattatttt attagatgaa
      541 gtaaaagatg ataagctcaa gcagaaaata gaaaacttta aatttttcgg tcaatatgca
      601 aatcttaaag aattgagaaa atataataat ggtgatgttt caattaatga aaatgttcct
      661 agttatgatg ttgaatacaa aatgagcaat aaagatgaaa tagttaagga attaagaagt
      721 cgttataata tttcgactga aaaatcacct atattaaaaa tgcatattga tggggacctg
      781 aaaggcagtt ctgtaggtta tagaaagtta gaaattgact tttcaaaacg tgaaaacagc
      841 aaattatcag tcattgaatt tttaagttat aaaccagcga aaaaatagta ttgataagga
      901 atattagggt gtgaaaaaat gcgatatcta aaaaagcttg catggttcat aagtgttatt
      961 attttgggca tttttataat aggttgtgat agttcaagcg atactgggga aaaagcaaaa
     1021 gaagattcaa aggaagaaca aatcaaaaag agctttgcga aaacattaga tatgtatcca
     1081 attaagaatc tcgaggactt atatgacaaa gaaggatatc gagatagcga atttaaaaaa
     1141 ggtgacaaag ggatgtggat gatatataca gatttcgcca aaagtaataa accgggtgta
     1201 ttagataatg aaggtatgat tttaaatttg gatagaaata cacgtacggc caagggatat
     1261 tattttgtag atactatata tgacaatcat gaaaactctt atagtaaaaa ttatagagtt
     1321 gagatgaaaa acaataaaat tattttatta gacaaggtgg aagatcaaaa acttaaagaa
     1381 agaatagaaa actttaaatt tttcggacaa tatgccgatt tcaagagttt gaaaagttac
     1441 aacaatggcg acgtttcaat taatagtaat gttccaagtt atgacgcgaa atttaaaatg
     1501 agtaataaag atgaaaatgt taagcaatta agaagtcgtt ataacattcc tactgaaaaa
     1561 gctccaatat taaaaatgca tattgatggg gacttaaaag gcagttccgt tggatataaa
     1621 aagttagaaa tagacttttc aaaagaagaa aatagcgaat tatcagtagt cgattcatta
     1681 aattttcagc ctgccaaaaa aaataaagat gatgaatgat agggaggatg tgaaacaatg
     1741 tggtcaataa aaagaattgg aatgtacaca aatgttataa ttttgagcgt ttttataata
     1801 ggttgtgata gttcaagcaa taatgcagaa aatcaaagag aagactcaaa agaagaacaa
     1861 atcaaaaaga gctttgcgaa aacgttagat atgtacccta ttaagaatct cgaggattta
     1921 tatgacaaag aaggatatcg tgatggtgag tttgaaaaag gcgataaggg gacgtgggta
     1981 ttgtattcag ctatggtatc acaaccaaaa ggcgagagtt taaaatcacg aggaatgata
     2041 ttaaagttag atagaaataa gagaactgct aaaggaagtt atattattag agaattgaaa
     2101 gaagataaaa atcatgatgt tcaaaaaaat gaaaagaaat atccagtgaa attggtgaat
     2161 aataggatag ttttgataaa agatgttaaa gacaaaaagt taaaaaaatg aaatagagtc
     2221 gtttgaatta ttttcacaat atggaaactt
//
LOCUS       DAMQYT010000271         2234 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.278, whole genome shotgun sequence.
ACCESSION   DAMQYT010000271 DAMQYT010000000
VERSION     DAMQYT010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.278"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            274..1209
                     /gene="lukE"
                     /locus_tag="PGA33_002632"
     CDS             274..1209
                     /gene="lukE"
                     /locus_tag="PGA33_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HDG6474372.1"
                     /translation="MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKR
                     TEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRM
                     IWPFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFN
                     YSKTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGS
                     AREYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRT
                     GIYAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
     gene            1211..2194
                     /gene="lukD"
                     /locus_tag="PGA33_002633"
     CDS             1211..2194
                     /gene="lukD"
                     /locus_tag="PGA33_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HDG6474373.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
BASE COUNT          865 a          306 c          389 g          674 t
ORIGIN      
        1 ttttgaagat ttgattaaag tcgtttttat ttttacagag aaaagattga gtatcggtaa
       61 ttttatcata ttgttcgaat ataaagggaa ctatttaaag gtaatgaagg ttgcgccaaa
      121 atgaccggtt ttcagcagca taaatgaata aattgtgtca tttttaatat tttatagcat
      181 tttatttttt aatgaacagt taattttata aataagtgaa ataatctaga ataaatttgt
      241 attaaaaatt tatatagaaa gaaagtgaaa cttatgttta agaaaaaaat gttagctgca
      301 actttgtcag taggactgat tgcaccttta gcatctccga ttcaagaatc tagagcaaat
      361 actaatattg aaaatattgg tgatggtgct gaagtaatca aacgtacgga ggatgtaagt
      421 agtaagaaat ggggcgttac tcaaaatgtc caattcgact ttgtaaaaga taaaaaatat
      481 aacaaagacg ctttaattgt taaaatgcaa ggttttatta attccagaac ttcattttca
      541 gatgtgaagg gtagtggata tgaattaact aaacgaatga tttggccatt ccaatataat
      601 ataggactga cgactaaaga tccaaatgtt agcttaatca attaccttcc taaaaacaaa
      661 atagaaacta ctgatgttgg tcaaacatta ggatataaca ttggaggtaa tttccagtca
      721 gcaccatcta taggtggcaa tggctcattt aattattcta aaacaattag ttatacccaa
      781 aagagttatg tcagtgaagt agacaagcaa aactcaaaat ctgttaaatg gggtgttaaa
      841 gcaaacgaat ttgttacgcc tgatggaaaa aaatctgcgc atgatagata tttattcgta
      901 caaagtccaa atggtccaac aggttcagca agagaatatt ttgctcctga taatcaattg
      961 ccacctttag ttcaaagtgg ctttaatcca tcgtttatca ctacactatc acatgaaaaa
     1021 ggttcaagtg atacgagtga atttgaaatt tcatatggta gaaacttaga tattacatat
     1081 gcgactttat tccctagaac tggtatttac gcagaaagaa agcataatgc atttgtaaat
     1141 agaaactttg tagttagata tgaagttaat tggaaaacac acgaaattaa agtgaaagga
     1201 cataattaat atgaaaatga aaaaattagt caaatcatca gttgcttcat caattgcact
     1261 gcttttgcta tcgaatacag ttgatgcagc tcaacatatc acacctgtaa gcgagaaaaa
     1321 agtagatgac aaaatcactt tatacaaaac aacagcaaca tctgataatg ataaattgaa
     1381 tatttctcaa attttaacgt ttaatttcat taaggataaa agttatgaca aagatacgtt
     1441 agtacttaag gcagccggaa acattaattc aggttataaa aagcctaatc caaaagatta
     1501 caattactca cagttttatt ggggcggtaa gtataatgtt tcggttagtt cagaatcaaa
     1561 tgatgctgta aatgttgttg actatgcacc taaaaatcaa aatgaagaat tccaagttca
     1621 acaaacatta ggttattctt atggcggaga tattaatata tctaatggct tatcaggtgg
     1681 attaaatgga tcaaaatcat tttcagaaac gataaattat aaacaagaaa gttacagaac
     1741 tacgattgat agaaaaacaa atcataaatc aattggctgg ggtgttgagg cgcacaaaat
     1801 tatgaataat ggttggggac catatggtag agatagttat gacccaacat atggtaatga
     1861 actgttttta ggcggtagac aaagtagttc aaatgctggt caaaatttct tgccaacaca
     1921 tcaaatgcct ttattggcgc gtggtaactt taacccagaa tttataagcg tactttctca
     1981 taaacaaaat gatacaaaaa aatctaaaat caaagtaact taccaaagag aaatggatag
     2041 atatactaat caatggaatc gactacactg ggttggtaat aactacaaaa atcaaaatac
     2101 agtaacgttt acatctactt atgaagttga ctggcaaaac catactgtta aattaatcgg
     2161 tacggattct aaagaaacta atcctggagt ataactgtta gaaatagagg aagttaaacc
     2221 cgaaatttaa gagt
//
LOCUS       DAMQYT010000272         2232 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.279, whole genome shotgun sequence.
ACCESSION   DAMQYT010000272 DAMQYT010000000
VERSION     DAMQYT010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.279"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..736
                     /gene="lpl8"
                     /locus_tag="PGA33_002634"
     CDS             <1..736
                     /gene="lpl8"
                     /locus_tag="PGA33_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HDG6474374.1"
                     /translation="KDEQKKEEQTNKTDSKEEQIKKSFEKTLDMYPIKNLEELYDKEG
                     YRDGEFKKGDKGMWTIYTDFAKSNKQGGLSNEGMVLYLDRNTRTAKGHYFVKTFYNKG
                     KFPDRKNYKVEMKNNKIILLDKVEDTNLKKRIENFKFFGQYANLKELKNYNNGDVSIN
                     ENVPSYDAKFKMSNKDENVKQLRSRYNIPTDKAPVLKMHIDGNLKGSSVGYKKLEIDF
                     SKGGKSDLSVIDSLNFQPAKVDEDDE"
     gene            755..1540
                     /gene="lpl9"
                     /locus_tag="PGA33_002635"
     CDS             755..1540
                     /gene="lpl9"
                     /locus_tag="PGA33_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl9"
                     /protein_id="HDG6474375.1"
                     /translation="MKSIKRIGLCISLLILIIFVTSCDGDNKIIGDSKEEQIKKSFAK
                     TLDIYPIKNLEDLYDKEGYRDGEFKKDDKGTWLIRSEMKIQLKGENLESRGAVLEINR
                     NTRTAKGHYIVREVVEDSDGMTHNHTKRYPVKMENNKMIPLKPIDDEKVKKEIEEFNF
                     FVQYGNFKELENYKEDEVSYNPEVPIYSAKYQLKNSDYNVEQLRKRYNIPTQKAPKLL
                     LKGSGNLKGSSVGYKNIEFTFIENKEENIYFTDSIYFNPSEDK"
     gene            1723..>2232
                     /gene="lpl10"
                     /locus_tag="PGA33_002636"
     CDS             1723..>2232
                     /gene="lpl10"
                     /locus_tag="PGA33_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl10"
                     /protein_id="HDG6474376.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEE
                     QIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTILTGFSKSNKPGVLDDE
                     GMVLYLNRNAKKATGYYFVNKVYDDISKNHNEKKYRVELKNNKIVLLDNVEDKKLKQK
                     IENFKFFSQY"
BASE COUNT          977 a          225 c          414 g          616 t
ORIGIN      
        1 gaaagatgaa cagaaaaaag aagaacaaac aaataaaaca gattcaaaag aagaacaaat
       61 caaaaagagt tttgagaaaa cattagatat gtatccaatt aagaatctcg aggagttata
      121 cgacaaagaa ggataccgag atggcgaatt taaaaagggt gataaaggga tgtggacgat
      181 atatacagat ttcgccaaaa gtaataaaca aggtggattg agtaatgaag gtatggtctt
      241 atacttagat agaaatacac ggactgcaaa gggacattat tttgttaaga cattctataa
      301 taagggcaaa ttcccagata gaaaaaatta taaagttgaa atgaaaaata ataaaattat
      361 cttattagat aaagtagaag atacaaatct aaaaaagaga atagaaaact ttaaattttt
      421 tggacaatat gcaaacctta aagaattgaa aaactacaac aatggtgatg tctcaattaa
      481 tgagaatgtt ccaagttatg acgcaaaatt taaaatgagc aataaagatg aaaatgttaa
      541 gcaattaaga agtcgttata atattcctac tgataaagca ccggtattaa aaatgcatat
      601 tgatggtaat ttgaaaggaa gttctgtggg ttataaaaag ttggaaattg acttttcaaa
      661 aggtggaaaa agcgatttgt cagtaataga ttctttgaat ttccagccgg cgaaggtaga
      721 tgaagatgat gaatgatgag gatggtgtgt aacaatgaag tctataaaaa ggattggatt
      781 gtgcattagt ttgttgattt taatcatctt tgttacatct tgtgatggtg ataataagat
      841 cattggagat tcaaaagaag aacaaatcaa aaagagcttt gcgaaaacgt tagatatcta
      901 ccctattaag aatctcgagg atttatacga caaagaagga tatcgagatg gcgaatttaa
      961 aaaagatgat aaaggtactt ggctaattag atctgaaatg aaaatccaat taaaaggaga
     1021 aaatctggaa tctagaggag cagttttaga aattaacaga aatactagaa cggctaaagg
     1081 gcattatatt gttagagaag ttgttgaaga tagcgatgga atgacacaca atcatacaaa
     1141 aagatatcct gtgaaaatgg aaaataataa aatgattcca ttaaaaccaa tcgatgacga
     1201 aaaagtaaaa aaagaaatcg aagaatttaa cttctttgta caatacggga atttcaaaga
     1261 attggaaaac tataaagagg acgaagtgtc atataaccca gaagtaccaa tttactctgc
     1321 aaaatatcaa ttgaaaaaca gtgattacaa tgttgaacaa ttaaggaagc gatataatat
     1381 cccgacgcaa aaagcgccca aattattatt gaaaggctca ggtaatttaa aaggttcatc
     1441 agtcggatat aaaaatattg aatttacctt tattgaaaac aaggaagaaa atatttactt
     1501 cacagatagt atctacttta atccaagcga ggataaataa atataattac taatataaaa
     1561 aataacgtta gaacatttaa taataaagtt aaaatgaatt acatgtatta aattgaataa
     1621 gttagttttg tttgataaca taaaagttga ataatcacta ttttaataag ttgtatgaaa
     1681 tattcaaatg tttataaaat taaaacagag agatgtgaaa tgatggagta tataaaaaaa
     1741 attgctttgt acatgagtgt attactttta atcattttta ttgggggatg tggaaatatg
     1801 aaagatgaac agaaaaaaga ggaacaaacg aataaaacag attcaaaaga agaacaaatc
     1861 aaaaagagtt ttgcgaaaac gttagatatg tatccaatta agaatctcga ggatttatac
     1921 gacaaagaag gatatcgaga tggtgagttt aaaaaaggcg ataaaggtac gtggactata
     1981 cttacaggtt tttcaaaaag taacaaacca ggagtattag atgatgaagg catggtgtta
     2041 tatcttaata gaaatgccaa aaaggcaaca ggttattatt ttgtaaataa agtttatgat
     2101 gatattagca aaaatcataa tgagaaaaaa tatcgtgttg aactaaaaaa taataagatt
     2161 gttcttttgg ataatgtaga agacaaaaaa cttaaacaaa aaattgaaaa ttttaaattt
     2221 tttagtcagt at
//
LOCUS       DAMQYT010000273         2227 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.280, whole genome shotgun sequence.
ACCESSION   DAMQYT010000273 DAMQYT010000000
VERSION     DAMQYT010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.280"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..1963
                     /locus_tag="PGA33_002637"
     CDS             <1..1963
                     /locus_tag="PGA33_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDG6474377.1"
                     /translation="RLGYLQIAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVD
                     NASKMAITYTRGRKTTQSEMLDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAM
                     MTKEQAMLADGSIKQDQYDKQLLSKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQM
                     IKNEDVSEKEYAAVSQQLSKLPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKEL
                     TEHYLSKGYSRNDRVGKSYLEYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQ
                     DLKLTIDIDLQKEVEALLDKQIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINK
                     SGKMTDYDIGTFTSQFAVGSSVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSY
                     FNKNGHVTINDKQALMHSSNVYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQ
                     VGLGVKTGIDLPNETRGQIEPLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYR
                     IQPHIGLTIHESTNKDEVGPLKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTG
                     YVSFKDTVVPTAGKTGTAEVFQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPW
                     LTGGDLGRDVINYYFKQLGKNDKNKDKDK"
     gene            2076..2225
                     /gene="rpmG"
                     /locus_tag="PGA33_002638"
     CDS             2076..2225
                     /gene="rpmG"
                     /locus_tag="PGA33_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDG6474378.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
BASE COUNT          873 a          326 c          428 g          600 t
ORIGIN      
        1 acgtttaggt tatttacaaa tcgcacaagg ctcacattat aaacaaatta taaaaaatga
       61 tgaaaacatt acagtgaatg agtctgtgcc aagaggtcgt attttagaca gaaatgggaa
      121 agttttagtt gataatgctt ctaaaatggc tattacatat actaggggtc gaaaaacaac
      181 acaatcggaa atgttggata cggctgaaaa gttatcaaag ctaatcaaga tggatactaa
      241 gaaaattaca gaacgtgata agaaagattt ctggattcag ttgcatccta aaaaagcaaa
      301 agcaatgatg acaaaagaac aagctatgtt agcagatgga agtattaaac aagatcaata
      361 tgataaacaa ctgttatcga aaatcggaaa atcacaatta gatgaattgt cttctaaaga
      421 tttacaagtt ttagctattt ttcgagagat gaatgcagga acagttttag atccacaaat
      481 gataaaaaat gaagatgtca gtgaaaaaga gtatgcagca gtttctcagc aactttccaa
      541 attaccaggt gttaacacgt ctatggattg ggatagaaaa tatccatatg gcgatacttt
      601 aagaggtata ttcggagatg tatcgacacc tgctgaaggt attccaaaag aattgacaga
      661 acattactta tccaaaggat attcacgcaa tgatcgtgtt ggaaaatctt acctagaata
      721 tcaatatgaa gatgtattgc gtggtaagaa gaaagaaatg aaatacacaa cggacaaatc
      781 tggtaaagtt acatcttcag aagtgttaaa tcctggcgct cgcggtcaag atttgaaatt
      841 aacgatcgat atagatcttc aaaaagaagt agaagcatta ttagataaac aaattaagaa
      901 gcttcgcagt caaggtgcca aagatatgga taatgcaatg atggttgtac aaaatcctaa
      961 aaatggagac attcttgcgc ttgccggaaa gcagattaat aagagtggta aaatgactga
     1021 ttatgacatt ggtacgttta cttctcaatt tgcggttgga tcttctgtaa aaggtggaac
     1081 attattagcc ggttatcaga ataaagctat caaagttgga gaaacaatgg tcgatgaacc
     1141 attacatttc caaggtggtt tgacaaaacg atcatacttc aataaaaacg ggcatgtaac
     1201 tattaatgat aagcaagctt tgatgcattc atcaaacgta tatatgttta aaacagcatt
     1261 aaaattagcg ggagaccctt attattctgg tatggcttta ccttcagaca taagttcacc
     1321 tgcccaaaag ctaagaagag gattaaatca agtaggctta ggtgtgaaaa cagggataga
     1381 tttaccaaat gaaacaagag gtcaaatcga accattaaca aataatccag gtaattatct
     1441 agatttatca attggtcaat atgataccta tacaccatta caattatcac aatatgtttc
     1501 aactatagcg aatgatggtt atagaataca gccacacatt ggattaacga ttcatgaatc
     1561 aactaataaa gatgaggttg gtccactcaa gaagaaaatt aatggcactg tcttgaacaa
     1621 ggttaataat actgaaaagg aaatcaaaca aattcaagaa ggattcaaaa tggcatttaa
     1681 tgataaagat ggtactggat atgttagttt taaagataca gtagtaccta ctgctggtaa
     1741 aacgggtacc gcagaagtgt tccaaaacgg agagccaaga gttaactcta cttatatagg
     1801 atacgcgcca attgatgatc caaaattagc gttttcaatt gtatatacaa atcagcctgt
     1861 accaccacca tggttaacag gtggagactt aggtagagat gtaattaact actactttaa
     1921 gcagttaggt aaaaatgata aaaataaaga caaagacaaa taaaatttaa cctgacgatt
     1981 gtgtagcgca tggttgtaaa attttaactt tgcaaatatt atagatgttg gtataataat
     2041 aaagtcgtat ttagaaatga taaggaggaa taaccatgcg cgtaaacgta actttagctt
     2101 gtacggaatg tggtgacaga aactacatta caactaagaa caaaagaaat aatccagaac
     2161 gtgttgaaat gaagaaattc tgttcacgtg aaaacaaaca aactttacac cgtgaaacaa
     2221 aataatc
//
LOCUS       DAMQYT010000274         2212 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.281, whole genome shotgun sequence.
ACCESSION   DAMQYT010000274 DAMQYT010000000
VERSION     DAMQYT010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.281"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            38..388
                     /gene="sarV"
                     /locus_tag="PGA33_002639"
     CDS             38..388
                     /gene="sarV"
                     /locus_tag="PGA33_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HDG6474379.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(490..1701)
                     /locus_tag="PGA33_002640"
     CDS             complement(490..1701)
                     /locus_tag="PGA33_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDG6474380.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFLVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(1694..2134)
                     /locus_tag="PGA33_002641"
     CDS             complement(1694..2134)
                     /locus_tag="PGA33_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HDG6474381.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLREAHND"
BASE COUNT          806 a          334 c          389 g          683 t
ORIGIN      
        1 acaaacatct aagaagaaac ataggaggca tcatattatg agtaataaag ttcaacgttt
       61 tatagaagca gaaagggagt taagtcagtt aaagcactgg ttaaaaacaa cacataagat
      121 ttcaattgaa gaatttgtag tcctttttaa agtgtatgaa gctgaaaaga ttagcggtaa
      181 agaattgagg gatacattac attttgaaat gctatgggat acaagtaaaa tcgatgtgat
      241 tatccgtaaa atctataaaa aagagcttat ttctaaattg cgttctgaaa cggatgaaag
      301 acaagtattc tatttctata gtacttctca aaagaaattg ttagataaaa ttactaaaga
      361 aatagaagtg ttaagcgtta caaactaaaa acttaaaaag catgccaatc agtacatcat
      421 aattgcgtct tggggacaga caaatgatga atagagattg gcatgctttt tatttttgaa
      481 tataaatatt tagttcatgg catttctagt tacatgacgt ccatgaatta agaagtaaac
      541 aagcatagta atgattgcta aagcggccat aaagccgaag atttcactat atgaaaacat
      601 atgagtaaat aacccaagga atgatggacc gaagccgaca cctgcatcta gaccaacgta
      661 aaaagtagat gtcgcgatac catatttaat cgggggtgag acttttatcg caatagattg
      721 cattgcagat gataaatttc cataccctaa acctaggcaa gcaccagcaa gtaatattaa
      781 ccagctttga tagcttgaaa ttaagcatac aaatgaaagg aaaagcatga taaatgctgg
      841 gtagacaata atattttcat ttttatcatc catcaatcta ccagcaatag gtctagtaat
      901 taacgatgct atagcatagc aaataaagaa atagcttgct gcagtgacta ggtgtcgctc
      961 taaagcaaat gcttgtaaat aagttaggat ggacgcatag gtaacgccaa ttaaaagcat
     1021 aattacagca acaggaatgg cctcttttgc aataaattga tgaatactaa atcttggttt
     1081 atcaatgaca ttagtttcag ttttgttatt tgttacttcg aaatcaactt ttataaataa
     1141 tgagataatg agtccgagta tgcctaatat gacacaaata ataaacagta agtcaattgc
     1201 gtattttgta ataagtaaca tgcctagaaa tgggccaatc gctgtaccta atactaaact
     1261 taaggaaaat aaactgatgc cttcactttt tctattaaca ggggtaacgt atgccgcaat
     1321 agtacctgtt gcagttgtca caactgcagt tgcgataccg tttatgagac gtacaaagat
     1381 taaaaaagct aaagatccat caataaaata aagtaattgc gtgataatta aagcaattaa
     1441 accaataaat aataatcgtt taggtccgat ttgatttaca aatttacctg tagcaaatcg
     1501 accaattaaa gacccgacaa taaatagtcc tacaactaat ccggctaatg aatcggaagc
     1561 gtcgaaagct tgcttactat agccagcaat aactacaagt aacaaataca tgcataggta
     1621 tacgataaag ttgactaaaa agttaattgt aaaacttttg gtgaaaatag gtgatttatt
     1681 taatgagcct tgattagtca ttgtgtgcct cccttaataa tatttcatga atttgattca
     1741 tcacatttat ggtttcttgc atttgatctt gcgaaatgtt tgttttggca actactgctt
     1801 gttggagctt ttgtacatca agacaaacaa tctcatataa ttcatgccct ttaggtgtta
     1861 aagttaaaaa cttttgtcgt ttatcctcta aactgttttc tactgttata aggtcatttt
     1921 ctattagagc ttttaaagtt tttcttgcag taggcttttc aattgcacgt ctatgtgaaa
     1981 tttcaatgag agtagtgggc tgatgtttag cgatatcgcg taagattaac cattggccat
     2041 aatatatatt gtgtttttct aaaatcggct cggctaattt taaatagggg cgatatattg
     2101 ttataaaact attgaaaaat tcttgtgata gcatagtgac atctcctaag acaaaatagt
     2161 tagcttagct aaccttttta caacaatagt aattataaaa cgggagcaat ta
//
LOCUS       DAMQYT010000275         2199 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.282, whole genome shotgun sequence.
ACCESSION   DAMQYT010000275 DAMQYT010000000
VERSION     DAMQYT010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.282"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(342..2150)
                     /gene="pepF"
                     /locus_tag="PGA33_002642"
     CDS             complement(342..2150)
                     /gene="pepF"
                     /locus_tag="PGA33_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDG6474382.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLETDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
BASE COUNT          674 a          383 c          299 g          843 t
ORIGIN      
        1 tttttcgaat ttaaccagtt cgaaaattat acaaattata aatttggtac ataatagaca
       61 tatatgaatc gttaatgtat aaaaagtgca tatttaatac atttttatac aacttcactt
      121 ccctcttatt aaaaaagaac ataaaaaaac gtcgtgtctg aatacacgac gctaaacaaa
      181 ggggatggga gaaatttttc acttcaaaca aaggggtatg tttgttatgt gattaatttc
      241 atgttcataa tataacatgc tatctctacc atttcaacct tttaatagta cttaatttaa
      301 attgtcacaa tctgttcata atgtaagcga atacaatagt actaagcttt cattaatttt
      361 tcaaaagcgt tcaatttttg ttcaaaaact tcacaagctt gttcaattgg ttcaggtgtt
      421 gtcatatcta caccagcatt ttttaatatc tcaattggat aatttgagct accttttttc
      481 aagaattcat taatatatct atctactgct ggcttacctt ctgttaaaat ttgatgactt
      541 aagctttgag ctgcactgta accagttgcg tattgatata cataataatt catatagaag
      601 tgtggaatac gtgaccattc cttactaata tcttcatcag tttctacaga atcaccgaag
      661 tataatttat ttaatttggc atattcttca ttcattctag ttggcgttaa tggttcacct
      721 gcttcttcaa ttgcatgaat tttatgctca aattctgcga acattgtttg tcggaataat
      781 gtagctctga aacgttctaa ttcttggttt aataataata agcgtttttc atcatcaaga
      841 tgtttatcca tataatcact taaaagtgct tcgttacaag ttgatgcaac ttcagcgaca
      901 aaaatagtgt agtcactaga atttgacggt tggaattttc tactgaagta actatgtgct
      961 gaatgcccaa attcatgaac taatgtgtat aagtctgaaa tagtattaga ccagttaagt
     1021 agaataaatg gattagttaa atgtgcacct gatgaatagc cacctgaacg tttaccttta
     1081 ttctcataga catcgaccca acgattgttt aagccttctt taactacatt taaatattct
     1141 tcacccattg gttctaatgc ttttaacatc cactctttag cttcttcata aggcatttca
     1201 aacttaatat ctttaattaa tggtgtatat aaatcataca ttttcaagtc atctaaacct
     1261 agcaattctt tgcgcaattc agtatatcta tgtagcaatg gtaaatattt atgtacagtt
     1321 tttactagat tgtcatatac attttctgga atatgattat tactcaatgc tttttctctt
     1381 gcagttttgt aattgtgtgt acgagcatta aatacatttt tcttcacttc acctgctagc
     1441 gtagcgccaa gcgtattatt atgagcacca tatgctttat atacatttct aaaagcactt
     1501 tctcttagtt tgcgatcatc tgtttctaaa tacttaataa atgtaccttg tgttaacggg
     1561 tgtgcatttc catctttatc tatcgcatct tcaaatacta aatcagcgtt gctaaacata
     1621 ccgtatacat ttgatggcgt tgataacgcg tcctgcgctt ctgttaataa cttttcagtt
     1681 tcagcatcta aaatatgagg acgtttttca ttaatcaact tcaaatcaaa tgcgaatttt
     1741 tgtaatttat catatgaatt tacaaatgat tgaattttat cttcatcaat ttgtaaaatc
     1801 tctggcacta agaaactcca tgccgagcta aatttaataa ttaattgatg tgctcttgac
     1861 tccataccag tatacttgtc gttcgttgta tcttggtctt gttttaggtg cgcgtataca
     1921 tatacttttt ctaatttagt acctaatgta tcttctaatt ctaacgcatt gtataatgtc
     1981 tcagcactat caccaatgtg tcctttaaat tgttcttctt tgcctaactc attttcaact
     2041 tctttaaatg cagcctcaaa agcttcatca tctttgaaaa ttgttgttaa gtcccatgta
     2101 tattcaggat atttacgttc ctgttcttct ctcgataatt gttgactcat atgtagtaaa
     2161 cctcctaata aaatataatt gatataattt tctcatttt
//
LOCUS       DAMQYT010000276         2176 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.283, whole genome shotgun sequence.
ACCESSION   DAMQYT010000276 DAMQYT010000000
VERSION     DAMQYT010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.283"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..166
                     /locus_tag="PGA33_002643"
     CDS             <1..166
                     /locus_tag="PGA33_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDG6474383.1"
                     /translation="LSNGPLEGINNKIKLIKRTSFGYGSYNHLRNRILLCSKLYAPKS
                     KKEVKQCLVA"
     gene            complement(144..245)
                     /locus_tag="PGA33_002644"
     CDS             complement(144..245)
                     /locus_tag="PGA33_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474384.1"
                     /translation="MQGFKEKHQELKKALCQIGLMRSISEVKQLNIA"
     gene            complement(368..463)
                     /gene="pepA1"
                     /locus_tag="PGA33_002645"
     CDS             complement(368..463)
                     /gene="pepA1"
                     /locus_tag="PGA33_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HDG6474385.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            666..809
                     /locus_tag="PGA33_002646"
     CDS             666..809
                     /locus_tag="PGA33_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDG6474386.1"
                     /translation="MTRERRSFSSEFKLQMVRLYKNGKPRNEIIREYDFTPSTFVNGG
                     YKM"
     gene            1413..1796
                     /locus_tag="PGA33_002647"
     CDS             1413..1796
                     /locus_tag="PGA33_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474387.1"
                     /translation="MSNQNLFDELEKKGYKLEDIFTKEEIKKYKAEDQLRAGKTQYVE
                     TGKDTATLYLSSAYTKTIAALGAGAISVISALTGGLVGAGVGGFLGSIAASNIDTSKG
                     IYIKLKTKKNAAGEYVLTGEKWGYQ"
     gene            1807..1983
                     /locus_tag="PGA33_002648"
     CDS             1807..1983
                     /locus_tag="PGA33_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474388.1"
                     /translation="MDRLKYSLKVGILALLLFCTLNYLVPMQSNAFSIIIYSAIFAVL
                     LMLLVYIFLGILKK"
     gene            1985..2170
                     /locus_tag="PGA33_002649"
     CDS             1985..2170
                     /locus_tag="PGA33_002649"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474389.1"
                     /translation="MKQISIGYEEIYGDRIFHSHFLKEHEVKNWKTVNQIIEKEHLDK
                     NEYEAEPPLFFFSGSLD"
BASE COUNT          824 a          294 c          382 g          676 t
ORIGIN      
        1 tttatcaaat ggtcctttag aaggtataaa taacaaaata aaattaatta aacgtacatc
       61 ttttggttat ggaagttaca atcatttgcg caatcgaata ttattatgtt caaaacttta
      121 cgctccaaaa agtaaaaagg aagttaagca atgtttagtt gcttaacttc ggatattgaa
      181 cgcatcagtc caatttgaca tagagccttt tttagttctt gatgtttctc tttaaaacct
      241 tgcatatttt acaaaaagaa aaattagcag tataattaag acaacgaaaa taagtattta
      301 cttatacacc aatcccctca ctatttgcgg tagtgagggg atttttattg gtgcggctat
      361 atgtcaccta ttttgtattg cgtctactta gccaataaga aaaaaacgca atggcacagc
      421 cactgatgac tggtgctatg atgtgaacga aaataagcat caccttatac acctcctctc
      481 tgcgtctaaa ttgacgcctg agagataggc gactctacta ttatatcatc ggcaaatata
      541 caagcacagt cacttgcttc tgataagtta tatgattcta gctgatagat tgaatcgtct
      601 acacttaatt ggacaaattc tatgagaata gatattgtta atttaagaaa gtaggcgatt
      661 ttattatgac aagagaaaga agatcattta gttcagagtt taagttacaa atggttagat
      721 tatataaaaa tggtaagcct aggaatgaaa ttatacgcga gtatgatttc acaccttcga
      781 cgtttgtaaa tggcggttat aaaatgtagg aaaatggata aagcaacatc aaaacacggg
      841 tacattcaat caccaagata acttatcgga tgaagaaaaa gagctgatta aattacgcaa
      901 agaagttcaa catttaaaaa tggagaacga tattttaaag caagtagcgc tgattatggg
      961 gcaaaaatag aagtcattca aaagaatgca catcaatatt cagtatcagc aatgtgtaaa
     1021 gtcctgataa tactaagaag tacctattat gattctataa aaagaaaaga taataaaatc
     1081 actaaagatg attcaaacat agaacatgcc gtcataaata tttttaattc taatagaaaa
     1141 gtctttggta caagacgaat taaaaatcat ttaaatgaca agggtctcac tgtatctgga
     1201 caaaagatag gtcgatcatg aaaaaatcta gtttctgttt atacgaaatc taaatacaaa
     1261 aatcatctaa aagaaactaa tgaaaaacga attaaaaatc tttattatta gctgctggtg
     1321 tattatttgt tagtccaatt tcattatctt ataattcaga tgtagctcat gctgaagata
     1381 agttagacca ttctcaagca aaggtaatat atttgagtaa ccaaaattta tttgatgaac
     1441 ttgagaaaaa aggttataaa ctggaagata tatttacaaa agaagaaata aaaaaatata
     1501 aagctgaaga ccaattgaga gcgggtaaaa ctcaatatgt agaaacaggt aaagatactg
     1561 caacattata tctttcttct gcatatacaa aaacaatagc tgctttaggt gcgggggcta
     1621 ttagcgttat ttctgcacta acaggaggtt tagttggcgc aggtgttggt ggtttccttg
     1681 gatctatagc tgcatcgaat attgatacta gtaagggaat atatataaaa ttaaaaacta
     1741 aaaagaatgc agccggggaa tacgttctga caggagaaaa atggggatat cagtaagggt
     1801 gattttatgg atagattaaa atattcactt aaagttggaa ttttagcatt attattattt
     1861 tgtactttaa attatttagt tccaatgcaa agcaatgctt tttcaataat tatatattcg
     1921 gcaatttttg ctgtgttact tatgctttta gtttatatat ttttaggaat tttaaagaaa
     1981 tgacatgaaa caaattagca ttggctatga agaaatctat ggggatagaa tttttcatag
     2041 ccatttttta aaagagcatg aagtaaaaaa ttggaaaacc gtcaatcaaa taattgaaaa
     2101 agaacacttg gacaaaaatg aatatgaagc ggaaccgccg ttatttttct tttcaggatc
     2161 gttagattag attcgg
//
LOCUS       DAMQYT010000277         2104 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.285, whole genome shotgun sequence.
ACCESSION   DAMQYT010000277 DAMQYT010000000
VERSION     DAMQYT010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.285"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..1136
                     /locus_tag="PGA33_002650"
     CDS             <1..1136
                     /locus_tag="PGA33_002650"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDG6474390.1"
                     /translation="QMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGEDFNAQLI
                     ALYNNSQGIPTKYISPKEAGIFVTDVPQEAQILDSAYSEIKKLRDYDEKLIIPGFFGV
                     SHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKDPELIEEITY
                     REMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVISG
                     ISCDKDFTVINIKKYLMNRQVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQ
                     NKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGTANKITHALAESKINLKM
                     INQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN"
     gene            complement(1190..1765)
                     /locus_tag="PGA33_002651"
     CDS             complement(1190..1765)
                     /locus_tag="PGA33_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474391.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
BASE COUNT          762 a          310 c          326 g          706 t
ORIGIN      
        1 tgcaaatgga tgaaagtatt ttacggacga tagatgtgac tttggaacat tatattaatc
       61 aattaaaaaa tgaaccaaag agactattag atgcattact ttcttgtggt gaagatttta
      121 atgcgcaatt gatagcttta tataataata gtcaaggtat accaacaaaa tatatatcac
      181 cgaaggaagc gggtattttt gttactgacg tcccacaaga agcacaaata ttagatagtg
      241 cttactctga aattaaaaaa ttaagagatt atgatgaaaa actaattatc ccaggatttt
      301 ttggcgtatc tcatgaagga tatatagtta catttccacg tggcggatca gatataactg
      361 gcgccatcat atcaagtggt gttagagcca caatttatga aaatttcacc gatgtgtctg
      421 gtatttataa agctaatcca aatatcatca aagatcctga attaatcgaa gaaatcactt
      481 atcgagaaat gcgtgaatta tcttatgctg gttttagtgt tttccatgat gaagccttac
      541 aaccacttta taaagatcgc atccctgtgg tgattaaaaa tacgaaccgc cctcaagata
      601 agggtacatt tatagtgcat gatagagaaa taaatgcgaa aaatgtgatt agcggtataa
      661 gttgtgataa agattttacc gttattaata tcaaaaagta tttaatgaat agacaagtcg
      721 gctttactag aaagatatta ggtgtcttag aggataataa tatttcattt gatcatatgc
      781 cttcaggtat tgatactatc agtatcgtta tgcgttcaaa acaaattcaa aataaagaaa
      841 caaaagtatt aaatgaaatt cgtcaaaaat gtgatgttga tgagctaagt attgagcatg
      901 atttagcaat attaatgatt gtcggcgaag gtatgaataa agtcatcggt actgcaaata
      961 aaattacaca tgccttagct gaatcaaaaa ttaacttaaa aatgattaac caaggtgctt
     1021 cggaaatctc gatgatgttt ggaattcatg tagatgatgc tgaaaaagct gtattatcta
     1081 catatgagtt ttgctatcac ggggttgcat taaaaaactt gtgtgataaa aactaatata
     1141 aaagcaattc gtaacacatg tattcaactg cgttatgaat tgctttttct taattattta
     1201 ttgttctctg gattgcatcc ttaatacgtt gctcacctac tacaaatttt aaaataaaaa
     1261 attgatagta gcttaatgca tctatgactc gtttataatt ggaataatga tgatactgat
     1321 ggtgttgcat tagatcatac attagaacga tctccggttt aatataatcc acatcccaat
     1381 ttaaactatg gaagtatatg ttactcttag gtatacgtat attcggatcc aatctaaaaa
     1441 tccattcatc gtttactaca tcataaacga aaatactcat tattgttgta tcatcatatt
     1501 gcacataaac ttttgcaatt tttgaaagtg ataaatcttc tttagctatt gcttgatctt
     1561 gcttatctat gtatagtaac gcatatgatt gcggaataac tttcacaata tcacttaaac
     1621 gcttacgttc cacgcatatt tcaacaatag aaggtaattt taattcatca tttaagaaaa
     1681 gctgcatggc aacttcaccg tgaaatttaa ataattgcga tgatgttttc attaaattgt
     1741 ccactaattg ttctttagca ttcatttcat tcactctcaa atacacctcc taagcaagcg
     1801 ctttcacaaa tatcattata cattttaaaa ttaatgaatt caatcttcaa aatactatga
     1861 gtgacctaat tctgtcattt aaatgaccgt tataacataa attcgacata aacttgaatt
     1921 cacgaatctt tcacgtcata ttcattgaac atcaatatct gtcagtttat aatttgatta
     1981 taacaatgat gaactaaaat caactaataa aaatgtggga aattgaataa caattaatct
     2041 ttattaatta ttattgtata caaagttaaa gaagtcaaaa aggattggtg aaaaaatgtc
     2101 tttt
//
LOCUS       DAMQYT010000278         2075 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.286, whole genome shotgun sequence.
ACCESSION   DAMQYT010000278 DAMQYT010000000
VERSION     DAMQYT010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.286"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(127..1293)
                     /gene="norA"
                     /locus_tag="PGA33_002652"
     CDS             complement(127..1293)
                     /gene="norA"
                     /locus_tag="PGA33_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HDG6474392.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANDYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
     gene            1502..1924
                     /locus_tag="PGA33_002653"
     CDS             1502..1924
                     /locus_tag="PGA33_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDG6474393.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
BASE COUNT          749 a          376 c          279 g          671 t
ORIGIN      
        1 ttagtcaatc gaatcttttt aaatgtaaag catcgaattt tcttaataaa attcataaga
       61 aaaacgatgc taatcattca taatattacg ctttacttta tatcaatata caccaaaata
      121 cttatgctac atattttgtt ctttcaattt tgctctatgt tgcttttcaa ttaaaacaat
      181 aacaacacct gctaatgaaa cacctatagc catataaatt ggtgcttcaa tgtgtacatc
      241 aaataacgca cctgcgatta aaggacctat gaaattaccc atactagtga atgtcgagtt
      301 caatccgcct gcaaagcctt gcctttctcc agcaatatta gaaaaataat ttgtaatggc
      361 tggtcgtatc atatcaaaac ctatgaagac aacaaaactg attaacatta ttgaccaata
      421 gtcattagca aaaactaata atattaagac aacaactgaa tataataatg accaagctat
      481 aaatgttaac tctgagaaat acttcataaa tttatcgaag aaatagattt ggaaaagtgc
      541 cccaaatata ccgccacccg taatagcaat cgaaatatct ttaggtgaat aatttacctt
      601 gtcagctgtg tatagtgaat acaatgtttc aaatgcagat aaaccaaacg ataatacaag
      661 tgttaaaata actggtgtaa taaacacttt ccagttaatt ttcgttagca attgtggctc
      721 taacttttgg aaaccacttg tcgtagactt tttcggatcg tgaatcaata caattgacat
      781 tataaatgct agaataccta atgctcctgc aaagtaaaat ggcatacgat gtgaaacttc
      841 tgccataaat ccaccaatcc ctggtcctaa aatgaatcca gaattgataa tcgctgacat
      901 gtagccaaag ttttttgctt tttgatggct tggtgaaatg tcagctatta aacctgtcac
      961 accaggcatt accataccag cactcatacc accaatcact ctcgataaca tcaataccga
     1021 aaaattgtgg ccaactgcaa acataaattc tgacactgaa aacaaaatta atcctataca
     1081 tataattaat ttcttcccta atttgtcagc tagcgtacca ccaaacggcg atataatcat
     1141 ttgagataac gcaaaagcag caactagtaa tcctaaatca ctaccagtta atcccaaatc
     1201 ttttaaataa acaggcaaga ctggtattac taaaccgata cctaaaaaaa tcaagaaaat
     1261 attaaaatat aagacaaaaa tctgtttatt catatgctca cctctttttt ctccatgtca
     1321 tgcttaaagc tgttattctt cttttccacg acagattgca attcatgcta aatattcgta
     1381 aaaagtttct atattgtatt acaacattgc tacatttgac aatatttttt ctttgtaatt
     1441 atcactattt tccatttaat tgtataatta atgacatatt aaaataaagg ggattgatca
     1501 tatgaaattg caaaaaagta atcatactat actactagtg ttggaaaaag gagaagatat
     1561 cgttgaatgt ataacaacct ttgcagatga ccaagactta acatttacat ctgtcagcgg
     1621 cattggtgca tgtgatgacg ttgtattaaa atttttcaat ttaacaacga aacaatacga
     1681 agaaaaacat attactgagc cactggaatt gacgagctta ttaggtaata tttctcgtct
     1741 agacaatggt cattttgcac atttgcatgc tactttcggt actcaatcat atgaaacttt
     1801 tagcggacat ctcgctaaag caattgtttc tgcaactgct gaaatcattc ttactgtaac
     1861 tgatttggac attcaacgtt cattcaaaga tgcagtcggt ttaaatttac ttgatcctca
     1921 atagcacttt actttttagt tgaatcaaat tttattacaa gttatcataa gtgcttaatc
     1981 ataaatgcat tctcgtttat taatatactt caacgagtta aattaaattt ttaattagga
     2041 attgaatgcg tttataacta tcacatttga atgaa
//
LOCUS       DAMQYT010000279         2066 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.287, whole genome shotgun sequence.
ACCESSION   DAMQYT010000279 DAMQYT010000000
VERSION     DAMQYT010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.287"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..338)
                     /gene="deoC"
                     /locus_tag="PGA33_002654"
     CDS             complement(<1..338)
                     /gene="deoC"
                     /locus_tag="PGA33_002654"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDG6474394.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYHFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVV"
     gene            653..1363
                     /gene="deoD"
                     /locus_tag="PGA33_002655"
     CDS             653..1363
                     /gene="deoD"
                     /locus_tag="PGA33_002655"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDG6474395.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            complement(1484..1927)
                     /locus_tag="PGA33_002656"
     CDS             complement(1484..1927)
                     /locus_tag="PGA33_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDG6474396.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
BASE COUNT          641 a          362 c          320 g          743 t
ORIGIN      
        1 accactgctt caatgtcttg ttgtacatca tcaaaacgtc catcttttaa tgcgccgatg
       61 ttgatgacca tgtcaatttc atctgcacca ttttgaatcg catcttctgt ttcaaatgct
      121 ttcgtcgcag ttgtcgatgc acctaatggg aatcctatta ccgtacaaac taacacctct
      181 gaatcagcta gtcgctctgc tgcatattta acatgcgttg gattcacaca tacagattta
      241 aaatggtatg ctttcgcttc atcgatgatt tgatcgattt gcgtacgtgt tgactcaggc
      301 ttcaataaag tgtgatcaat caattttgca ctattcattt tctatctcct cctttatggt
      361 tgattataaa aatacggttg taaattagtt gattgagcgt caggttcatt taaatatcag
      421 gttagatgtt cgctttttat gtaaccgcat acatatacta ttacattaat tcatttccca
      481 taaacaaaca atacaattga acgtgatatc ttcattatga acgatgactt gacaacaagc
      541 taatcaggat tatattttta taattcttta attctatagt acaaaaattc gcaaaaaagg
      601 gaaacaaatg ttatcttaaa attattaatg aatattaagg agaagataac aaatgacaaa
      661 aggtacacca catattcaac caaatggagt aaaaattgct aaaacagtat taatgcctgg
      721 cgatccgcta cgtgcaaaat atattgctga taatttttta gaaaatgttg aacaatttaa
      781 cgatgtacgt aacatgtttg gttacactgg tacatataaa ggtaaagaag tttctgtaat
      841 gggttctggt atgggtattc caagtattgg tatttactca tatgagttat acaacttctt
      901 tgatgtagat acaatcattc gtatcggttc ttgtggcgca ttacaagaaa atgttaactt
      961 atacgatgtt attattgcac aagctgcatc aactaattca aattatgtag atcaatacaa
     1021 tattccaggt catttcgcgc ctatcgctga cttcgagtta gtaactaaag ctaaaaatgt
     1081 cgctgaccaa atcggtgcta ctacacacgt aggtaacgta ttatcttctg atacatttta
     1141 caatgccgat ccaacattca atgatgcttg gaaaaaaatg ggtattttag gtatcgaaat
     1201 ggaatcagct ggtttatatt taaatgcgat tcatgctggt aaaaaagcac ttggtatttt
     1261 cacagtaagt gatcatattt tacgtgacga agctactaca cctgaagaac gtcaaaattc
     1321 atttacacaa atgatggaaa tcgctttaga aatcgcagag taacttattt aaattgactt
     1381 taattgctct ttaacaatgc gattaaactc aaaaagccaa cacattctgg gcgtatcccc
     1441 atttatgtgt tggcttttat ttatattatt acttatctgt agattagctt aagtaagatt
     1501 taaacatcca attatgttta tctactgatg tttgcatacc tataaacata tcttctgata
     1561 catcatcgcc agcattacca gcaatttcga ttgcgttttc taattgtttt gagatatttg
     1621 tgaagtcttg tgataattct tcaaccattt gttctgcaga gtaacctttc gcagcttctt
     1681 taacaattga ttgctctaag cattcagtta atgtacctac agggtttcct cctaccgcta
     1741 aaattctttc agctaattcg tctacatatt ggcttgcttc attatataat tcttcaaatt
     1801 taacgtgtaa tgagaagaag ttaggccctt tcacatacca gtggaaattg tgtagctttg
     1861 tgtaagctac tgtccagttt gctacttgtt gattcaattc ttttacaaca tcttgttgat
     1921 tactcatatt taatacactc cttaaaattg tctacgtctt gcttgtttat attaattatt
     1981 atattataat aattctaatc tgtaaagtgt ttttgctctc ttttttataa ttattctaat
     2041 ctaaccgtaa agtgttccgc tattta
//
LOCUS       DAMQYT010000280         2040 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.288, whole genome shotgun sequence.
ACCESSION   DAMQYT010000280 DAMQYT010000000
VERSION     DAMQYT010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.288"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..658
                     /gene="arlR"
                     /locus_tag="PGA33_002657"
     CDS             <1..658
                     /gene="arlR"
                     /locus_tag="PGA33_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HDG6474397.1"
                     /translation="TQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSHY
                     YDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKPF
                     DIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLAE
                     NKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVIR
                     "
     gene            655..2010
                     /gene="arlS"
                     /locus_tag="PGA33_002658"
     CDS             655..2010
                     /gene="arlS"
                     /locus_tag="PGA33_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HDG6474398.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
BASE COUNT          790 a          275 c          351 g          624 t
ORIGIN      
        1 gacgcaaatt ttaatagtag aagatgaaca aaacttagca agatttcttg aattggaact
       61 cacacatgaa aattacaatg tggacacaga gtatgatgga caagacggtt tagataaagc
      121 gcttagccat tactatgatt taatcatatt agatttaatg ttgccgtcaa ttaatggctt
      181 agaaatttgt cgcaaaatta gacaacaaca atctacacct atcattataa ttacagcgaa
      241 aagtgatacg tatgacaaag ttgctgggct tgattacggt gcagacgatt atatagttaa
      301 gccgtttgat attgaagaac ttttagcaag aattcgtgca attttacgtc gtcagccaca
      361 aaaggatatt atcgatgtca acggtattac aattgataag aacgctttta aagtgacggt
      421 aaatggcgca gaaattgaat taacaaaaac agagtatgat ttactatatc ttctagctga
      481 aaataaaaac catgttatgc aacgggaaca aattttaaat catgtatggg gttataatag
      541 tgaagtagaa acaaatgtcg tagatgttta tataagatat ttacgaaaca agttaaaacc
      601 atacgatcgt gacaaaatga ttgaaacagt tcgtggcgtt gggtatgtga tacgatgaca
      661 aaacgtaaat tgcgcaataa ctggattatt gttaccacga tgattacgtt tgtcacgata
      721 tttttgtttt gtttaattat tatttttttc ttgaaagata cactgcataa tagtgagctt
      781 gatgatgcag aacgaagctc aagcgatatt aataatttat ttcattctaa gcctgttaaa
      841 gatatatctg cattagactt gaatgcatct ttaggtaatt ttcaagagat aattatttat
      901 gatgagcata ataataaatt atttgagaca tcgaatgata acacagtgag agttgaacca
      961 ggttatgaac accgttattt tgaccgcgta ataaaaaaac gctataaagg cattgaatat
     1021 ttaattatta aagaaccaat tacaacgcaa gatttcaaag ggtatagctt gttaattcat
     1081 tcactagaaa attatgataa catcgtaaaa tcattgtata tcattgcgct ggcatttgga
     1141 gtgattgcaa caattataac tgccacaatc agttatgtat tttcaacaca aattactaaa
     1201 ccgcttgtca gtttatcaaa taaaatgatt gagattcgac gagatggttt tcaaaataaa
     1261 ttgcaattaa atacaaatta tgaagaaata gataatttag caaatacgtt taatgagatg
     1321 atgagccaaa ttgaagaatc atttaatcaa caaagacaat ttgttgaaga tgcgtcacat
     1381 gaattacgaa caccattaca aattattcaa ggtcatttaa atttgattca gcgatgggga
     1441 aaaaaagacc cagcagtatt agaagaatcg ttaaatattt ctattgaaga aatgaatcgt
     1501 atcataaaat tagtcgaaga attacttgaa ttgactaaag gagatgtaaa tgacatttct
     1561 tctgaagcac agaccgtgca tattaatgat gaaattcgct cgcgaataca ctcattaaaa
     1621 caattgcatc ctgattatca atttgatacg gatctgacat ctaaaaatct agaaattaaa
     1681 atgaaacctc atcaattcga acaattattt ttaatcttta ttgataatgc aatcaaatat
     1741 gatgtgaaga ataagaaaat taaagttaag acaaggttaa aaaataagca aaaaataatt
     1801 gaaattacag atcatggaat tggtattcca gaggaagatc aagatttcat ttttgatcgc
     1861 ttttatcgag tggataaatc tcgttcaaga agtcaaggcg gtaatggact cggattatct
     1921 attgctcaaa aaatcattca attaaacgga ggatcgatta aaattaaaag tgaaattaac
     1981 aaaggaacaa cgtttaaaat catattttaa tcatgactga gacgtcaatc aaagtcatag
//
LOCUS       DAMQYT010000281         2030 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.289, whole genome shotgun sequence.
ACCESSION   DAMQYT010000281 DAMQYT010000000
VERSION     DAMQYT010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.289"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..525
                     /gene="sdpA"
                     /locus_tag="PGA33_002659"
     CDS             <1..525
                     /gene="sdpA"
                     /locus_tag="PGA33_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpA"
                     /protein_id="HDG6474399.1"
                     /translation="VVSEVHLLYSMLIVLVGLILLRLFKINLFSFKKLTLSQVIYIIG
                     GALLIYGLDNLYLYFHDVPANEQQLEQAIRNTPFYISIFTVTIIPAIVEEIVFRGMII
                     RVIFRKHLFLGLIVSSLVFASLHESDTWIGYLPYLYSGLIFGLIYIKTKRLEVVIFMH
                     FLNNLLALLFIIWR"
     gene            complement(965..1678)
                     /locus_tag="PGA33_002660"
     CDS             complement(965..1678)
                     /locus_tag="PGA33_002660"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDG6474400.1"
                     /translation="MAKLNVEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
BASE COUNT          633 a          335 c          261 g          801 t
ORIGIN      
        1 gttgtcagtg aagtacattt actttatagc atgctaattg tattagtagg tttaattcta
       61 ttgaggctat tcaaaatcaa tttattctct tttaaaaaat taacattgtc tcaagttatt
      121 tatattatag gcggtgcact attaatttat gggttagata atctttattt atattttcat
      181 gacgtaccgg caaatgaaca acaattagag caagcaatac gaaatacacc attctatatt
      241 tctattttca ctgttaccat catccccgct attgtggaag aaattgtttt tcgcggtatg
      301 ataataaggg ttatcttcag aaaacacttg tttttagggt taattgtgtc tagtttagtt
      361 tttgcatcat tacacgaatc tgacacttgg attggttatt taccttactt atattctggt
      421 ttgatttttg gcctaattta tataaaaaca aaacgattag aagttgtaat attcatgcac
      481 ttcttaaata acttgttagc tctgctcttt ataatatgga gataattttg agataacctt
      541 tcatattatt ttcaaattat ctatctgata tttattttat tgaacttgag tcaccactta
      601 aactcaaaaa tacgtgtcat caagtgcaaa ataatttaga actaatacaa acataaaaac
      661 ttttacttta cgcttaaaat caaaagtcac atacctagat ataagtacaa cttgtatatt
      721 ttgtactcta ataggtatgt ggcttaaata atatttaatt cttgagttgc atatagtaac
      781 tgtttttatt cattttctaa aatatgcaag actaatttcc ttttctactc ctgttgaatc
      841 aatttagact tttttataac tcgagctttt acttagctga attttgactt cttcttcatt
      901 aataactatg atggaaaaac ccccataagc acaatgccta tgagggttta aatatattta
      961 tatattatag aatagaaaga cctgaagatt gaatatcttt cgcaaagcct ttaactgtat
     1021 ctactgataa ttcgttaata tcgcgaccta agtttgtatt cacttttttc acaacatctg
     1081 ctgggcatgt aataatatct gcaccaattt catcagcttg aatcacattg aataattcgc
     1141 ggcaacttgc ccataataat ttaacgccgt ctttactatg cgtaactttt acagcctctt
     1201 tcattaatgg taatggatct acgcctgtat ctgcaatacg tcctgcaaat actgaaacat
     1261 atgttggcac accttcagtt actgcttcag ttatttcttt aacttgttca attgtgtaaa
     1321 cagccgtaac gtttaatctc acattgtcag ctgaaagttt tttaattaaa ggaatcgttg
     1381 attcaccttt tgtatttaca ataggaattt taacaaatac attttcgcca tattgtttta
     1441 aaattgctgc ttctttttcc atagtttcta aatcgtctgc aaatacttca aatgaaattg
     1501 aagcatctgg aatttctttc acagcttctt cagcaaaagc tttgtaatct gttacgcccg
     1561 ctttagccat taaactagga tttgttgtaa aaccatccac ttgtttgttt ttataagctg
     1621 ctttcatttc ttcaatatct gcaccgtccg caaatacttc tacatttagt ttagccatat
     1681 aatatagcct ccttgattct tattaaaatt ttaacaacat ctgcatgtct ttttcttaca
     1741 accatttgta aaaaatgatt tttatttctt tgttatataa aacatttata tcacgctttt
     1801 ataatatata gctaattttt tgcctatatt ttttaaaaat gactttagca aacattgtgt
     1861 ctacaagtta ataaaactat tgttttatga ttgtttatga tacgatatgt aaatgataat
     1921 catccaggag gtctagcatg tctcgttcaa aaaaatactt ttacttatct agcttaatga
     1981 ttattttaag ctttttcttt aatacaaata acgttttcct aagtggactt
//
LOCUS       DAMQYT010000282         2007 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.290, whole genome shotgun sequence.
ACCESSION   DAMQYT010000282 DAMQYT010000000
VERSION     DAMQYT010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.290"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            86..586
                     /locus_tag="PGA33_002661"
                     /pseudo
     CDS             86..586
                     /locus_tag="PGA33_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532440.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(646..1143)
                     /locus_tag="PGA33_002662"
     CDS             complement(646..1143)
                     /locus_tag="PGA33_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDG6474401.1"
                     /translation="MIINLETQQIHFDLEHEIPYFQAPEKNRIRLDIDVLNKKQISNI
                     INVIFNNQSKTKCTFLSEYLYPVKFREKTRIGRFFNITNWYEEIHSTDEKYVIATIKN
                     IKNKDLIDYSLYIKKGLVEPFIVFYNQENIFYISNAVIDIISVSKDTLDYIKSEFNDH
                     CASPY"
     gene            complement(1145..1231)
                     /locus_tag="PGA33_002663"
                     /pseudo
     CDS             complement(1145..1231)
                     /locus_tag="PGA33_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1246..1572
                     /locus_tag="PGA33_002664"
                     /pseudo
     CDS             1246..1572
                     /locus_tag="PGA33_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1601..1792
                     /gene="vraH"
                     /locus_tag="PGA33_002665"
     CDS             1601..1792
                     /gene="vraH"
                     /locus_tag="PGA33_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HDG6474402.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALTILVIVLSSFTTPMIGI
                     PAGLLGGSYYLKRREEKDK"
BASE COUNT          687 a          310 c          305 g          705 t
ORIGIN      
        1 cctattattt aaaacgtcgt gaagaaaaag gtaaataaat aagccttatg tgctaaactc
       61 aatatgtatt tcctaccaaa gaaaacataa ggagtttgga catcttgaag gagatactat
      121 tgttggtcgt cgtcatggta gtgcaatcat tactttggta gaaagagtat ccaagatgat
      181 tatcacctta cgacccttag gtcgtaaggc aactgatata gaaaacgctc tacactcaat
      241 gttttcatct ttaccttctc atatatttaa atcaattact tttgattgtg gtaaggagtt
      301 ctcaaactgg aaaacattat gtaactgtca cgatatatct atattttttg ctgatccagg
      361 cacgccatca cagcgaggtt taaatgaaca ttctaacgga atacttagac gcagtggatt
      421 agataaagaa ctagatttca atttagtaac agacgatcat atcatcagtg ttgctcaaaa
      481 aatcaatcat cgtcctagaa aatctttagg ttatagaaca ccattggaag tatttatgag
      541 tttcatcgaa gatgataaac ttgtccagct taatttgaca atctaaagat ttataaaaaa
      601 gccgttggtc tcttcgaaac tacggctttt ctctgtgcta taaaatcagt atggcgaagc
      661 acagtgatca ttgaattctg actttatata atcaagagta tctttggata cactgatgat
      721 atctataaca gcattgctaa tgtagaatat attttcttga ttatagaata caataaatgg
      781 ctccactaaa ccttttttaa tatataaaga ataatcaatt aagtctttgt tttttatatt
      841 tttaatagtt gcaataacat acttctcatc ggtagaatgg atttcttcgt accagttagt
      901 aatattaaaa aaacgaccaa ttcttgtttt ttctctaaat ttaacaggat ataagtattc
      961 agacaaaaat gtacatttcg tctttgattg gttattaaat ataacattaa taatattaga
     1021 aatttgcttt ttattgagaa catcaatatc taagcgtatc ctatttttct caggtgcttg
     1081 aaaataggga atttcatgtt ctaaatcaaa atgtatttgt tgtgtttcaa ggttaataat
     1141 cattttaatc tccaattctt tttaggttag catcgtatgt accacttctt gttttcttgc
     1201 tacctaaatt cttaatactg cttgcacctt ttcattttat cttctcatat atttaaatca
     1261 attacttttg attgtggtaa ggagttctca aactggaaaa caatatgtaa ctgtcacgat
     1321 atatctatat tttttgctga tccaggcacg ccatcacagc gaggtttaaa tgaacattct
     1381 aacggaataa ttagacgcag tggattagat aaagaactag atttcaattt agtaacggat
     1441 gatcatatca tcagtgttgc tcaaaaaatc aatcatcatc ctagaaaatc tttaggttat
     1501 agaacaccat tggaagtatt tatgagtttc atcgaagatg ataaacttgt ccagcttaat
     1561 ttgacaatct aaagatttat aaaaaaggag agattgaatc atgaaattta gagaattagt
     1621 aaaacaatca tatgaggatt tgaaaaattt gactgtaact tggtataacg tcattgcttt
     1681 aacaatactt gttattgtat taagctcatt cacaacacca atgataggca ttccagctgg
     1741 tttattaggt ggctcttatt atttaaaacg tcgtgaagaa aaagacaagt aagcacattc
     1801 catcggtata gaacattgct atgacctttc aagtcagatt ttaatatcaa caagtagcgt
     1861 ttatcgctgc ttgtttttta tggattgctt ataaaatcat catgtttaaa cgatatgtca
     1921 ctttgaaaac tagcaaacac cataaaagac tacaattttt ttatttttat aaaccccttt
     1981 aaatcaacgt ttctaaggtt tttaaaa
//
LOCUS       DAMQYT010000283         1946 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.291, whole genome shotgun sequence.
ACCESSION   DAMQYT010000283 DAMQYT010000000
VERSION     DAMQYT010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.291"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..186
                     /locus_tag="PGA33_002666"
     CDS             <1..186
                     /locus_tag="PGA33_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HDG6474403.1"
                     /translation="LQSYIGKLVKLTLTNNKILIGKVIDFDDKVDNFDGYNSIEIDTG
                     RITYDISENKIKTIVLQ"
     gene            complement(735..1760)
                     /locus_tag="PGA33_002667"
     CDS             complement(735..1760)
                     /locus_tag="PGA33_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HDG6474404.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
BASE COUNT          691 a          343 c          236 g          676 t
ORIGIN      
        1 ttacagtcat acataggcaa attagtcaaa ttaacgctta ctaataataa aatattaatt
       61 ggaaaagtga tagactttga tgataaagtt gataattttg atggttataa ttcgatagaa
      121 attgatacag gtagaattac atatgatata tcagaaaata aaattaaaac tattgtttta
      181 caataatagc atcctttcta cacagataaa gagaaagcgg tgctattttt tgcgctttta
      241 taacaactaa ggatacaaga cacataactt caacaatatc ctcatctaaa cctatcgatt
      301 tcctaagctc aattttaaac acaaagttta tttgaaatga tgcacaccta taaaaatctt
      361 aatcatcgaa aagtcacaaa catctccata ttaaaaactt aaatagtgca ttccatttaa
      421 actaaacatc gatattccta ccaatataga cttagtaatt cgattgttaa tacattactt
      481 tcataaaata actacaacca tcataatact tctcaaatgt cgtcaaatta aagtaaatac
      541 agcacaaatt aacattttta aatatataaa ataaaacaag atactcaata atctgtagta
      601 ccttgttttg gaataatgta attttatatc atcaacggct acaaatgagc agtttcatat
      661 ataaataaac acatctactc aactgatctt aattaattaa taaatgattt accagatgac
      721 atataacagc attattagat taattctaat agtttattta aattttcttc ggttgtcgcc
      781 caactggttg cgaatctaac aacacgatgt tgatcatcgt atttttccca aacagcaaat
      841 ttaacttttt gttctaactc tgctattttc tcgttactta aaataaaaaa ttgttgattg
      901 gttggagaat caaagtaaag acgatagcct ttatttttaa acccgtcttt catcttattt
      961 gccatttcga tagcatgtct gcttatatta aaatataaat tgtccgtaaa taattctaaa
     1021 aattgtatgc ctgttaaccg tccttttgct aaaagggcac cgtgatgctt gattcgagtg
     1081 gtaaattgtt tcggttcatt atttttcgta aaaacaatgg cttccccgca taatgcacct
     1141 atcttcgtac cacctatata aaatacatca caatatttag cgatgtcttt aatagtcata
     1201 tctgattggt cactcatcaa tccataccct aatcgtgcac catccataaa taatggaagc
     1261 tgatattgct tacatacttt gcataactct tccaattctg atttagagta taatgtgcca
     1321 tattctgtag gatgagaaat atataccatt cctgggaata ccatatggtc ctttttaaaa
     1381 tcacttttaa atgtctccat gtaagtttca acatctgaag cactaacttt tccttcctta
     1441 gagggtatag taattacttt atgtccacta tattcaattg caccgccctc atgcacagca
     1501 acatgaccag tgtctgctga aatgacccct tcgtaacttt ctaacattga attaataaca
     1561 acctgattgg tttgtgttcc acctactaaa aaacgaattg tagcatttgg acagtcaatt
     1621 gtatctttaa tcttttcaat tgcctgagct gtgaattgat caaagccata tcccgaagct
     1681 tgtacaagat ttgtatctac taatcgtttt aatacttttt catgagcacc ttctaaataa
     1741 tcattttcga atgaaatcac tacatttccc cctaaaacta atatcaacat tttaataaga
     1801 taaaccaatt tcaaaactag ttcgatattt aaaatgtatt atgaatggtt aaagtttgta
     1861 tcgcattatc gcgaagttga ataaatatat tatacatcaa acaatacatt atgaaaatta
     1921 attaattttt tatcacagcg actttt
//
LOCUS       DAMQYT010000284         1934 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.292, whole genome shotgun sequence.
ACCESSION   DAMQYT010000284 DAMQYT010000000
VERSION     DAMQYT010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.292"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(287..1933)
                     /locus_tag="PGA33_002668"
     CDS             complement(287..1933)
                     /locus_tag="PGA33_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDG6474405.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
BASE COUNT          696 a          379 c          287 g          572 t
ORIGIN      
        1 attgatttat gtcccagcct gagttaattt tctataaaag tatatttaat ttgcgtttat
       61 accgtcaaac ttcactttag ctttgtcaaa cccctttcta ttaagttttc agaaataaac
      121 ctatcttaaa atataaaaaa atcgagaatt cgtagtttaa taacgaaatt ctcgttctta
      181 tccttttgaa tatactcaat tttccacaaa aacaaacaag tagtatatct gttctagcta
      241 ctagaatgac atactacttg ttattaaaat acttaactaa actttattag ttatcttttt
      301 tctctatatt tctacgtgac tgacgctttt caagaatgtc agattcataa tcttcttgtt
      361 gactcttgat atattcttgt aagcgatgtt tattcggagt caatgttaaa cctaggaatt
      421 tacgttcctg gttcgcatcc ttgtagaaac ttaccatcat gagtatgacg acaaaggaga
      481 atgggaatgc acttataatt gcagcacttt gaatcgcatt taaagcttca gcgccgttac
      541 cgccaccagc taataaaagt acaaatgcta ttaaggcctg tgaaattccc caaacaactt
      601 ttaccatact agatggattt aatgaaccaa atgttgtttg cattcctaat acaaatgttg
      661 ctgagtcagc agatgtaata aagaatgatg caattaataa taatgcaatc aacgataaaa
      721 caatgccaaa tggcacatga ttaaacactc caaatagctg tgtttcagga gtcatatcaa
      781 aaatttcttt gtgtttctta cctgtctcga tgcctaatac accaaagaca ctaaaccaaa
      841 caaaactaac aattgctgga actagcaaga caccagaaat gaactctcta attgaacgtc
      901 cttttgaaac tcgtgcaata aacactccaa cgaatggact ccaacttaac caccaacccc
      961 agtaataaag tgtccatgaa gacatccatt cacgtttttg aggatttaaa gctgctgtat
     1021 caaaactatt aaacaagaat gtgtttagta aactacccgt agagctagtt aacatattta
     1081 aaataagaac agttggtcca acaattaaag cagctaccat taaaatagta cctaaaccaa
     1141 tgttcaagtt acttaagtat tgaatacctt tacttaatcc agaccatgca cttgctataa
     1201 ataagatagt aacaacaatg atgataatcg cttgtacaaa cgtattgttt ggaacattga
     1261 acaagtaatg taaaccacca ttaatttgta gagcacccat acctaacgaa acggctaccc
     1321 caacgattgt cgcaaataca gataaaacgt caataaaaat cccaatagga ccttctactt
     1381 tatcacctaa aagaggacgt aaagttctag ataataaacc tggttcacct ttacggaatt
     1441 gcgaatatgc caacgctaac gcaacaacac cataaacagc ccaagcatgg aatccccaat
     1501 ggaaaaatgt tgaacgtaga gcttcagtat aagcttcagt agttttggga tctgctgtag
     1561 gtggcgtagc aaagtgcgcc atcggttcag ctgcaccata aaacaccaaa cctatcccca
     1621 taccagcact aaacaacata gcaaaccatg aaattgtatt aaactcaggt ttgtcatttg
     1681 gtttacctag tttaagtttt ccaataggac taaaaataag gaatatacag aagaacacga
     1741 taatcgtagt aagaataaga taataccaac ctaacttttc tgtaatccac attttaatat
     1801 tattggtaac atagttgaat tgttcaggta aaaatgcacc aagtaatacg actatagcaa
     1861 caacaattgc actatagatg aagactggtg aatacttctt tccatttgga ttctctggtg
     1921 aagaagaatt cata
//
LOCUS       DAMQYT010000285         1915 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.293, whole genome shotgun sequence.
ACCESSION   DAMQYT010000285 DAMQYT010000000
VERSION     DAMQYT010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.293"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            19..957
                     /gene="lukS-PV"
                     /locus_tag="PGA33_002669"
     CDS             19..957
                     /gene="lukS-PV"
                     /locus_tag="PGA33_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     S"
                     /protein_id="HDG6474406.1"
                     /translation="MVKKRLLAATLSLGIITPIATSFHESKADNNIENIGDGAEVVKR
                     TEDTSSDKWGVTQNIQFDFVKDKKYNKDALILKMQGFINSKTTYYNYKNTDHIKAMRW
                     PFQYNIGLKTNDPNVDLINYLPKNKIDSVNVSQTLGYNIGGNFNSGPSTGGNGSFNYS
                     KTISYNQQNYISEVERQNSKSVQWGIKANSFITSLGKMSGHDPNLFVGYKPYSQNPRD
                     YFVPDNELPPLVHSGFNPSFIATVSHEKGSGDTSEFEITYGRNMDVTHATRRTTHYGN
                     SYLEGSRIHNAFVNRNYTVKYEVNWKTHEIKVKGHN"
     gene            953..>1915
                     /gene="lukF-PV"
                     /locus_tag="PGA33_002670"
     CDS             953..>1915
                     /gene="lukF-PV"
                     /locus_tag="PGA33_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     F"
                     /protein_id="HDG6474407.1"
                     /translation="MDMKKIVKSSVVTSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDSDKLKISQILTFNFIKDKSYDKDTLILKAAGNIYSGYTKPNPKDTISSQF
                     YWGSKYNISINSDSNDSVNVVDYAPKNQNEEFQVQQTVGYSYGGDINISNGLSGGGNG
                     SKSFSETINYKQESYRTSLDKRTNFKKIGWDVEAHKIMNNGWGPYGRDSYHSTYGNEM
                     FLGSRQSNLNAGQNFLEYHKMPVLSRGNFNPEFIGVLSRKQNAAKKSKITVTYQREMD
                     RYTNFWNQLHWIGNNYKDENRATHTSIYEVDWENHTVKLIDTQSK"
BASE COUNT          763 a          271 c          314 g          567 t
ORIGIN      
        1 agaaaggaaa tgatttttat ggtcaaaaaa agactattag ctgcaacatt gtcgttagga
       61 ataatcactc ctattgctac ttcgtttcat gaatctaaag ctgataacaa tattgagaat
      121 attggtgatg gcgctgaggt agtcaaaaga acagaagata caagtagcga taagtggggg
      181 gtcacacaaa atattcagtt tgattttgtt aaagataaaa agtataacaa agacgctttg
      241 attttaaaaa tgcaaggttt tatcaattca aagactactt attacaatta caaaaacaca
      301 gatcatataa aagcaatgag gtggcctttc caatacaata ttggtctcaa aacaaatgac
      361 cccaatgtag atttaataaa ttatctacct aaaaataaaa tagattcagt aaatgttagt
      421 caaacattag gttataacat aggtggtaat tttaatagtg gtccatcaac aggaggtaat
      481 ggttcattta attattcaaa aacaattagt tataatcaac aaaactatat cagtgaagta
      541 gaacgtcaaa attcaaaaag tgttcaatgg ggaataaaag ctaattcatt tatcacatca
      601 ttaggtaaaa tgtctggaca tgatccaaat ttatttgttg gatataaacc atatagtcaa
      661 aatccgagag actattttgt tccagacaat gaattacccc cattagtaca cagtggtttc
      721 aatccttcat ttattgcaac tgtttctcat gaaaaaggct caggagatac aagtgaattt
      781 gaaataacgt atggcagaaa tatggatgtt actcatgcta ctagaagaac aacacactat
      841 ggcaatagtt atttagaagg atctagaata cacaacgcat ttgtaaacag aaattacaca
      901 gttaaatatg aagtgaactg gaaaactcat gaaattaaag tgaaaggaca taattgatat
      961 gaaaaaaata gtcaaatcat cagttgttac atcaattgca ttgcttttgc tatccaatac
     1021 agttgatgca gctcaacata tcacacctgt aagtgagaaa aaggttgatg ataaaattac
     1081 tttgtacaaa acaactgcaa catcagattc cgataagtta aaaatttctc agattttaac
     1141 ttttaatttt attaaagata aaagttatga taaagataca ttaatactca aagctgctgg
     1201 aaacatttat tctggctata caaagccaaa tccaaaagac actattagtt ctcaatttta
     1261 ttggggttct aagtacaaca tttcaattaa ttcagattct aatgactcag taaacgttgt
     1321 agattatgca cctaaaaatc aaaatgaaga atttcaagta caacaaacgg taggttattc
     1381 ttatggtgga gatattaata tctctaacgg cttatcaggt ggaggtaatg gttcaaaatc
     1441 tttttcagag acaattaact ataaacaaga aagctataga actagcttag ataaaagaac
     1501 taatttcaaa aaaattggtt gggatgttga agcacataaa attatgaata atggttgggg
     1561 accatatggc agagatagtt atcattcaac ttatggtaat gaaatgtttt taggctcaag
     1621 acaaagcaac ttaaatgctg gacaaaactt cttggaatat cacaaaatgc cagtgttatc
     1681 cagaggtaac ttcaatccag aatttattgg tgtcctatct cgaaaacaaa acgctgcaaa
     1741 aaaatcgaaa attactgtta cttatcaaag agaaatggat agatatacaa acttttggaa
     1801 tcaacttcac tggataggta ataattataa agatgaaaat agagcaactc atacatcaat
     1861 ttatgaagtt gattgggaaa atcatacagt taaattaata gatactcaat ctaag
//
LOCUS       DAMQYT010000286         1900 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.294, whole genome shotgun sequence.
ACCESSION   DAMQYT010000286 DAMQYT010000000
VERSION     DAMQYT010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.294"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(40..732)
                     /locus_tag="PGA33_002671"
     CDS             complement(40..732)
                     /locus_tag="PGA33_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDG6474408.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            1117..>1900
                     /locus_tag="PGA33_002672"
     CDS             1117..>1900
                     /locus_tag="PGA33_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HDG6474409.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGY"
BASE COUNT          650 a          264 c          331 g          655 t
ORIGIN      
        1 aagatatttt ctaattgaaa atcatcttac tgctgttttt taggaatcga tggggtttaa
       61 aaattgatga tagttatcaa tggatatgtc tctggtttga atcgtcttac cgaatgaata
      121 cgaaatatct ataatatttt caatttcgag ttcggcagtt ttgtcttgag caatgtcata
      181 aatgactgca tgccaatgtc catgtttaca caaaaggcca ataaatataa cgttttcagc
      241 tttaatatta tcatcaaccc tatgatctac taacatcaca tttcgttctg tacaataaat
      301 taaaatgtcg gaaaacagca ttgggagtgt catatgttgt gtgtcatcaa atttaatata
      361 aaagtccatg cgtttaagca attttcttaa gcgtgtttgt ggaagactta aacattgttt
      421 tataattgcg ttgatttcgc ttttatatgg taatgtcgag tacgactgac tttcattaag
      481 cactaaaaat aaggctgata attgtgtttc agataagttt aatgcaattt tagattgtgc
      541 ttgttgtatt tggtaaccac catttttccc ataatgcgca taaattttaa caccttgatt
      601 ttctaaatca tcaatatctc ttaaaattgt gcgtttggat acgttgcaat atttagctaa
      661 ttctaaagcg gtcatttttt tattttgttg aattgcagta attattaaat tttgcctttc
      721 agctttattc atgacagcca cctcctaaaa gatgatatat tacacaaagt ataacataag
      781 agtgacaatg aggacatttt caatatcttt taaaaaaaat cgtaaaaatt ttgtggaaaa
      841 cgcttacatt ttaaaaaaga ttgtgctatg atattacttg taacaggggg agcgattaaa
      901 caaaggggta gagctatata acagatagct tttaatcgtt caagttacat cacaacatct
      961 tcaatattta ttacttactt tcctttctat ttgtcggcta gcacgtgact agccgatttt
     1021 tttatgcata aaatgaagaa gtcttcagtt gtattgttga ttcttttaat atagggtaat
     1081 aataaaatga taatgatatg agaagtggtg gacgaaatga cagataaaac attacagcaa
     1141 atagataagt taatatgttc gtggttgaaa caaatagaca atgttattcc gcaattaatt
     1201 atggaaatga ctacagaaac taagcgtcat cgatttgatt tagttacaaa tgtagataaa
     1261 cagattcaac aacaattcca acaattttta gcaacacatt ttccggaaca ccaactatta
     1321 gcggaagaaa aaagtaatga aatgattacg aatgaaatta atcatttatg gattatggat
     1381 cccattgatg gaactgccaa tttagtaaaa cagcaagaag attattgtat tatattggcg
     1441 tatttttatg aaggtaaacc aatgttatcg tacgtatatg attatccgca taaaaagctt
     1501 tataaagcaa tacgaggaga aggtgctttt tgtaatggaa ttaagatgga agaaccacca
     1561 tcgttaaaat tggaagacgc tattatatcg tttaatgcac aagtgatgaa tctagatacg
     1621 gtgcaagatt tatttgacgc ctcatttagt tatcgtttag ttggtgcttg cggattagat
     1681 tctatgcgag tggcaaaagg tcaatttggg gcacatatta atacaaatcc taaaccttgg
     1741 gacattgctg cacagttttt atttgcagaa ctattgaatc taaaaatgac gactttagat
     1801 ggtaaggcaa ttgatcattt aaagggtgca ccttttatta taagtaataa agcatgtcat
     1861 gaaacggtac ttaaaatttt aaatgcaaat ggtggttatc
//
LOCUS       DAMQYT010000287         1897 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.295, whole genome shotgun sequence.
ACCESSION   DAMQYT010000287 DAMQYT010000000
VERSION     DAMQYT010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.295"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(222..698)
                     /gene="folK"
                     /locus_tag="PGA33_002673"
     CDS             complement(222..698)
                     /gene="folK"
                     /locus_tag="PGA33_002673"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDG6474410.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK"
     gene            complement(695..1060)
                     /gene="folB"
                     /locus_tag="PGA33_002674"
     CDS             complement(695..1060)
                     /gene="folB"
                     /locus_tag="PGA33_002674"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDG6474411.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            complement(1038..1841)
                     /gene="folP"
                     /locus_tag="PGA33_002675"
     CDS             complement(1038..1841)
                     /gene="folP"
                     /locus_tag="PGA33_002675"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDG6474412.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAINRVKAMIDEGADII
                     DVGGVSTRPGHEMVSLEEEMNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
BASE COUNT          594 a          416 c          228 g          659 t
ORIGIN      
        1 taaaaattat cttccacaaa cttcaacaaa agcctaaata aattacagca atttatcaaa
       61 tattgcttac tttgatttta tgaaataact taattctaac acatactaaa tcatcatata
      121 ctaattcgaa atcaaatgca tttagagata atcaaaatgc ggaaacatct ccaataatca
      181 ataatctatt cccaataaat atgaatgttc tcaacaatac attatttata tctctttaca
      241 ctgtcatcga caaaaactaa atctttcact ttcaatttcg aacgtggttc tacgacattt
      301 gctgctatat catttaatgg gattaaaaca aatgcacgtt cattcattct cggatgtggc
      361 accgacagtt ttggtaaatc tatcatttct tctccataca acaaaatatc cacatctaaa
      421 gttctaggac cccatcgttc ctttctaata cggtgtaaac attcttctgt cttcaaacaa
      481 cattccaaca gttgtaatac tgtgagtgtt gtttgaattt caacacacaa atttaaaaag
      541 ttaggttgct cagtataccc aactggtgct gtttcataaa tcggagaaat attagatacg
      601 ttaataccat catattcatt caaaatcttt atagcatcgt ttaactggct ttctctatca
      661 ccaatattac tacctaatcc taagtatgct tgaatcattt attctccctc actatttcga
      721 tacctactcc atcataatga cccggaatcg gtgggttttc tttagtgatt ctcactttcg
      781 tttccattac acgattatat tgtgaattta tacgatttgc aatacgttca gctagatgct
      841 caagtaaatt aacggcctta ccttccataa ttgatttaac ctcttcgaac acttcaccat
      901 aatgaactgt atcaataaca ttatcagtac gcccagcttc agctaagtct actttcaaag
      961 ttacatccac tttgaaaatt tgccctattt cattttcagc tgataaagca ccatgatatc
     1021 cataaaagcg cataccttta agaaagattg tgtcttgcat tttcattctc ctttaaaaaa
     1081 tctatacctt tagctaattt agcattcaac tcgacattat gaacgcgtac tgctctaacg
     1141 cctttcataa taccatatgc agtcgtagct gcagttactt catctctttc aaccggtgtt
     1201 gtatcataac ccatcatctc tttagtgaaa cgtttccggc ttgtcgctaa taaaactgga
     1261 tattctgttg caacaagttc atccagtctt gccataactt cggcttcttc atttctagtt
     1321 ttagcgaaac ctatacctgg atctagccaa attttatttg aaggtatacc agctatttta
     1381 gcttgatgtg cttgtgctaa caaagatgtt aacatttctt cgacaaccgg ttcatcacga
     1441 ttaccatttc cattatgcat taaaataatt tccgcgtcat atttagctac aatttggaac
     1501 atacgatgat catacagacc cgcccattga tcattaatca tatcaacgcc taattttaaa
     1561 catgcttcag caacctcact tcgaaatgta tcgactgaaa tttttacatc aaaaccgaca
     1621 atagcttcaa caacaggtaa tactctgttc atctcttctt ctaatgaaac catttcatga
     1681 ccgggtctcg ttgaaacacc tccaacatct ataatgtcag caccttcatc tatcatggct
     1741 ttcactctat ttatagctga ttcaacatta ttaaattttc caccatctga gaatgaatca
     1801 ggtgtgacgt ttaatatgcc cataattttt gttttagtca tgttaaccac cctttaatta
     1861 tgtattgttt aactctaata aaatttactc ctgctat
//
LOCUS       DAMQYT010000288         1887 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.296, whole genome shotgun sequence.
ACCESSION   DAMQYT010000288 DAMQYT010000000
VERSION     DAMQYT010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.296"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..1383
                     /locus_tag="PGA33_002676"
     CDS             <1..1383
                     /locus_tag="PGA33_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDG6474413.1"
                     /translation="LNGFTLRKIIFENFSKKCTYWVTLIITQILLTTSVLWILNYTGN
                     LDLFILRIVLLSCLFILTISVINLWTFLMLLNLNIANMIKGKQHFKTIRFINTVCKSI
                     LLVLIANVMIENTSVIKDLNKIKETEKYWNVLDDYYTIEFAPYHETKQSLIDNMVRSE
                     QLVKASEAENNAILFKPKGDSVDNDNFSPDEGNVILVNNQFWSIYYKQFQPDIPIKNQ
                     KNNVEVIIPQKFHAMRNEINQAYHSWFEFVQNKNNKENKLSIQFINKNDCRIFSFDAR
                     DSRHLSFIEAPIIVNVQASDLSNDFYYAMISQGGYLFKNYDALVKNIEKYHLDGEISG
                     ITNYKDSVMEMYHENNLKLTVLNFSQIIIAIILIIIILFDVKYYFEQHRKLLVIKKLY
                     GYSTLRANYQYLLINNIVVIFIGILTNVILHSHYIMMLFATILVVQILLQICSLYYHG
                     RRFNEVIKEF"
     gene            1386..1706
                     /locus_tag="PGA33_002677"
     CDS             1386..1706
                     /locus_tag="PGA33_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDG6474414.1"
                     /translation="MKFIIAILLGLILSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKE
                     VRQE"
     gene            1703..>1887
                     /locus_tag="PGA33_002678"
     CDS             1703..>1887
                     /locus_tag="PGA33_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDG6474415.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNG"
BASE COUNT          720 a          234 c          292 g          641 t
ORIGIN      
        1 ttaaatggtt ttacattaag aaaaataatt ttcgaaaatt tttcaaagaa atgcacgtat
       61 tgggtaacgt taatcataac tcaaattctt ttaactacaa gtgtactttg gatattaaat
      121 tacacgggta atttagattt attcatatta agaatagttc tgctttcatg tcttttcatt
      181 ttaacgatta gtgttattaa tttatggact ttcttaatgt tactaaattt aaatattgca
      241 aatatgatta aaggtaagca gcactttaaa acaattcgtt ttattaatac agtttgtaaa
      301 agtattctct tagtactaat agctaatgta atgatagaaa atactagtgt tattaaagat
      361 ttgaataaaa taaaagaaac tgagaaatat tggaatgtat tagatgatta ttacacgatt
      421 gaatttgcac cttatcacga aacaaaacaa agtttgattg ataatatggt gcgatcagaa
      481 caattagtaa aggctagtga agcagaaaat aatgcgattt tattcaaacc aaagggtgac
      541 tccgttgaca atgacaactt ttcgcctgat gaggggaatg taatattagt aaataatcag
      601 ttctggtcga tttattacaa gcagtttcaa cctgatattc cgataaaaaa tcaaaaaaat
      661 aatgtcgaag taattattcc acaaaagttt catgcaatgc gtaatgaaat caatcaagca
      721 tatcattcat ggtttgaatt tgtacaaaat aaaaataata aagagaataa gttatctata
      781 cagtttatca acaaaaatga ttgtcgaatt ttttcatttg atgcacgaga tagtcgccat
      841 ttgtcattta tagaggcgcc aatcattgtg aatgttcagg catcagattt atcgaatgat
      901 ttttattatg ccatgatcag tcaaggcggg tatttattca aaaattatga cgcgctagta
      961 aaaaatattg aaaagtatca tcttgatggg gaaatcagtg gaataaccaa ttataaagat
     1021 agcgtgatgg aaatgtatca tgaaaacaat ttgaaattaa cagtactcaa cttttcacaa
     1081 atcattatcg caatcatttt aataattatt attttatttg atgtgaaata ttattttgaa
     1141 cagcatcgaa aattactcgt aatcaaaaag ctatatggtt attcaacatt aagagccaat
     1201 taccaatact tattaataaa taatatagtt gttattttta ttggaatatt gacgaatgta
     1261 attttacatt ctcactatat aatgatgtta tttgcaacga ttcttgtcgt tcaaatatta
     1321 ttgcagattt gcagcctata ctatcatggc cgacgtttta atgaagttat caaggagttt
     1381 taactatgaa atttatcatt gcaatattat taggattaat attatccatt actattgctt
     1441 ttacaatcat acatcatcct atacttgatc gttttaatcc tttcttaaaa acggagtata
     1501 gttatgccaa agtgccaaaa ggtacgcaac aatatgttaa tattacggct tatagtgaaa
     1561 gaggggaaaa gcttgattat aaattaacat ttaatggatt ttcacctagt agaacgtatg
     1621 tcgaaataaa gcataaaggg caatatgtca tatcgatcac atatgttgaa aaagaggata
     1681 caccaaaaga ggtaagacaa gaatgataga attaaaagat ttaaccatac aaaaaggtaa
     1741 tacacatatt ttgaagaaac ttaatttgaa atttcaatgt ggaaaatcat atgcacttat
     1801 tggtaaaagt ggatgcggta aatctacatt attaaatact attgctggac ttgaaaaaac
     1861 aggaaaacaa tatgtctatt ttaatgg
//
LOCUS       DAMQYT010000289         1868 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.297, whole genome shotgun sequence.
ACCESSION   DAMQYT010000289 DAMQYT010000000
VERSION     DAMQYT010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.297"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..307
                     /locus_tag="PGA33_002679"
     CDS             <1..307
                     /locus_tag="PGA33_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="immunoglobulin-binding protein sbi"
                     /protein_id="HDG6474416.1"
                     /translation="DTAKYYYNTYYKYKGAIDQTVLTVLGSGSKSYIQPLKVDDKNGY
                     LAKSYAQVRNYVTESINTGKVLYTFYQNPTLVKTAIKAQETASSIKNTLSNLLSFWK"
     gene            843..1772
                     /gene="hlgA"
                     /locus_tag="PGA33_002680"
     CDS             843..1772
                     /gene="hlgA"
                     /locus_tag="PGA33_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HDG6474417.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
BASE COUNT          735 a          324 c          248 g          561 t
ORIGIN      
        1 tgatacagca aagtactact ataatacgta ctataaatac aaaggtgcga ttgatcaaac
       61 agtattaaca gtactaggta gtggttctaa atcttacatc caaccattga aagttgatga
      121 taaaaacggc tacttagcta aatcatatgc acaagtaaga aactatgtaa ctgagtcaat
      181 caatactggt aaagtattat atactttcta ccaaaaccca acattagtaa aaacagctat
      241 taaagctcaa gaaactgcat catcaatcaa aaatacatta agtaatttat tatcattctg
      301 gaaataatca atcaaaaata tcttctctag ttttacatca ttttttaaat aattttcgta
      361 acaaaccgtg attaaaaaga accgttgatt ctcaatcgaa tctacggttc ttttttcatt
      421 ttccatcaat taaatgcttc ttcgctattt gtcagcccac ttttttacct gcaacttgtt
      481 aaataatcct tacatcgtta acgaatagtt catcatttag ttgaatcagc tcaactttat
      541 taacttcata ttttcacaaa ctattgcgca atccattcct tttccactac aagcaccata
      601 attaaacaac aattcaataa aataagactt gcaaagcata gttatgtagc tatataaacg
      661 cctgcgacca ataaatcttt taaacataac ataatgcaaa aacatcattt aacaatgcta
      721 aaaatgtctc ttcaatacat gttgatagta attaactttt aacgaacagt taattcgaaa
      781 acgcttacaa atggattatt atatatatga acttaaaatt aaatagaaag aaagtgattt
      841 ctatgattaa aaataaaata ttaacagcaa ctttagcagt tggtttaata gcccctttag
      901 ccaatccatt tatagaaatt tctaaagcag aaaataagat agaagatatc ggccaaggtg
      961 cagaaatcat caaaagaaca caagacatta ctagcaaacg attagctata actcaaaaca
     1021 ttcaatttga ttttgtaaaa gataaaaaat ataacaaaga tgccctagtt gttaagatgc
     1081 aaggcttcat tagctctaga acaacatatt cagacttaaa aaaatatcca tatattaaaa
     1141 gaatgatatg gccatttcaa tataatatca gtttgaaaac gaaagactct aatgttgatt
     1201 taattaatta tcttcctaaa aataaaattg attcagcaga tgttagtcag aaattaggct
     1261 ataatatcgg cggaaacttc caatcagcgc catcaatcgg aggcagtggc tcattcaact
     1321 actctaaaac aattagttat aatcaaaaaa actatgttac tgaagtagaa agtcagaact
     1381 ctaaaggtgt taaatgggga gtgaaagcaa attcattcgt tacaccgaat ggtcaagtat
     1441 ctgcatatga tcaatactta tttgcacaag acccaactgg tccagcagca cgagactatt
     1501 tcgtcccaga taatcaacta cctcctttaa ttcaaagtgg ctttaatcca tcatttatta
     1561 caacattgtc acacgaaaga ggtaaaggtg ataaaagcga gtttgaaatc acttacggca
     1621 gaaacatgga tgctacatat gcttacgtga caagacatcg tttagccgtt gatagaaaac
     1681 atgatgcttt taaaaaccga aacgttacag ttaaatatga agtgaactgg aaaacacatg
     1741 aagtaaaaat taaaagcatc acacctaagt aaacagttca atcatcttaa aaaatcctgg
     1801 gacacttcat acttgtctca ggatttttta acaaattgaa tcagccttat aacattaaat
     1861 tattttat
//
LOCUS       DAMQYT010000290         1851 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.298, whole genome shotgun sequence.
ACCESSION   DAMQYT010000290 DAMQYT010000000
VERSION     DAMQYT010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.298"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            309..>1851
                     /locus_tag="PGA33_002681"
     CDS             309..>1851
                     /locus_tag="PGA33_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDG6474418.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNGLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFR
                     VEDILT"
BASE COUNT          688 a          255 c          371 g          537 t
ORIGIN      
        1 aatgttagat gattgagcat tataccttaa taacatcgtt tatttatttc ataaattgta
       61 gtatcataga actaatattt aaaaaatgaa acagtagatt taggtcgaat ttttgtaaaa
      121 gttttaaaag taggaatagt atacaaatta aactcgctca agtaaaatta atattacgat
      181 taatgacgac aggataaata tttatcgtcg acggacgtat gattggtgtg ggacaaatac
      241 tattcaacaa gagtacctaa atcattgttt aaggcgaagt aataaatatg aatggggtgt
      301 atcatataat gaacaacgaa tatccatata gtgcagacga agttcttcac aaagcaaaat
      361 catatttgtc agcagatgaa tatgagtatg ttttaaaaag ctatcatatt gcttatgaag
      421 cacataaagg tcagttccga aaaaacggat taccatacat tatgcatcct atacaagttg
      481 caggtatttt aacagaaatg cgattagacg gaccgacgat tgtcgcaggt tttttgcatg
      541 atgtaattga agatacaccg tatacatttg aagatgtaaa agaaatgttc aatgaagaag
      601 ttgctcgaat tgttgatggt gtgacgaagc ttaaaaaagt aaaataccgc tcaaaagaag
      661 aacaacaagc tgaaaatcat cgcaagttat ttattgcgat tgccaaagat gtacgcgtaa
      721 ttttggtgaa attagcagac agattacata atatgcgtac cttgaaagcc atgccgcgcg
      781 aaaaacaaat tagaatttct cgagaaacat tagaaattta tgcaccatta gcacatcgtc
      841 ttggtattaa tacaatcaaa tgggaactag aagatacggc tcttcgttat attgataatg
      901 tgcaatattt tagaatagtc aatttaatga agaagaaacg tagtgaacgt gaagcgtata
      961 tcgaaacggc tattgataga atacgtactg aaatggaccg aatgaatatc gaaggcgata
     1021 taaatggtag acctaaacat atttacagta tttatcggaa aatgatgaag cagaaaaaac
     1081 aatttgatca aatttttgat ttgttggcga tacgtgttat tgtcaattct attaatgatt
     1141 gttatgcgat acttgggttg gtgcatacgt tatggaaacc gatgccagga cgttttaaag
     1201 attatattgc aatgcctaaa caaaatttgt atcagtcatt gcatactaca gtagtaggcc
     1261 caaatggaga cccgctcgaa atccaaatac gaacgtttga tatgcacgaa attgctgagc
     1321 atggtgttgc agcacactgg gcttacaaag aaggtaaaaa agtaagtgaa aaagatcaaa
     1381 cttatcaaaa taagttaaat gggttaaaag aattagctga agcggatcat acatcgtctg
     1441 acgctcaaga atttatggaa accttaaaat atgacttaca gagtgacaaa gtatacgcat
     1501 ttaccccagc gagtgatgtt attgagttgc catatggtgc tgtgccgatt gattttgctt
     1561 atgcgattca cagtgaagta ggtaataaga tgattggtgc caaggtgaat ggcaaaattg
     1621 taccaattga ctatatttta caaacaggcg atattgttga aatacgtact agtaaacatt
     1681 catatggacc aagtcgtgat tggttgaaaa ttgttaaatc gtctagtgcc aaaggtaaaa
     1741 ttaaaagttt cttcaaaaaa caagatcgtt catctaatat tgaaaaaggc cgaatgatgg
     1801 ttgaagctga aataaaagag caaggattta gagtcgaaga tattttgaca g
//
LOCUS       DAMQYT010000291         1841 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.299, whole genome shotgun sequence.
ACCESSION   DAMQYT010000291 DAMQYT010000000
VERSION     DAMQYT010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.299"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..1036)
                     /locus_tag="PGA33_002682"
     CDS             complement(<1..1036)
                     /locus_tag="PGA33_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HDG6474419.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLTNHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDN"
     gene            complement(1263..1841)
                     /gene="pgsA"
                     /locus_tag="PGA33_002683"
     CDS             complement(1263..1841)
                     /gene="pgsA"
                     /locus_tag="PGA33_002683"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDG6474420.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
BASE COUNT          634 a          336 c          222 g          649 t
ORIGIN      
        1 tattatcata gcctatatga actacaccat catactctaa tagaataatt gctgatgaca
       61 cattaaacaa tttattaaca aattgcactg catcttttaa ttgctgctca ataggtttat
      121 taatatcaac aaagttttca ttgtgattaa tcataccctt tagaacatcg tttaaatcca
      181 cttctttcaa tcgatgatat aaagcaccat tagtaatacc atcatatatt acaaaaggtt
      241 catgaatttt ctttattaca gcttgctcaa ttaatgtgtc atctgaacca taataatatt
      301 ctccaatacg atcaagaatt tcttgtttaa caggttgaat caatgattgt gcttgttctt
      361 ttgagtcggc atttgcagtc aatctaatat atacttcatg acttcccgcc aaaggcgcaa
      421 tcgtaggatt agtctgttta tcgataagat ctattaatat tgtttctact ttagattcac
      481 ctattcccgc aaatcttaat agttcagaat gtataattcg attatggttt ataaaatgtg
      541 acaacaattc atttttcacc attggttgca tttctttcgg tggacctggt aataaaataa
      601 tttgtttgtt ttcaaaattc accatcattc ctggagccat gccatgatga tttgttaata
      661 cagttgaacc ttcaattact aaagcctgtt gtttattatt aggtgtcatt tcttgtcctt
      721 gttcctcaaa atagctttca atatatttta aagaaggctc atcaataact aaatctttac
      781 caacaatctg ggccactgta tgcttcgtta agtcatcttt cgtaggacct aagccacctg
      841 ttaaaataac agtatcatat ttttctagcg catgacgtac acttgattct aaacgttttt
      901 tattatctcc aataacttta tgttctaata cattttgtcc aatttcatta aatactttag
      961 atagaaattg tccgttggta ttagcgattt gacctagcaa tagttctgag cctacagcaa
     1021 taatggcaat tgacatttat atcccccttg tatcgtttaa ttcatttttt catgtatttc
     1081 atttaaaaat tcacatcaat tttagattca atgtatcaga tgctactaca gattttccct
     1141 aaaaaaatga acaaatattg ttttacaata tatcattata aacaattgcg tggctaattt
     1201 ctaaaattgg gattaaaaaa actagattcc atatgaaaat gaaatctagt ataaacaaat
     1261 atttattttt gtttaaaaac atctctacct ttataaaagt attcaatacc agataagata
     1321 gtaaaaataa cgccaatgta taataaaatt tgtcctaatg acaaaccaat caatgttgcc
     1381 aatggatcac ctaataacaa ccaagtaatt gctaccatag taactgctgt tttaatttta
     1441 cctaattgac cagctgcact tacgaatcct tgttcaattt gtagtaaacg taaaccagtt
     1501 acggcaaatt ctctggcaat aataatgatt gctactacag aatttgttag tcctagttgc
     1561 acaagtacaa ttaaagcact tgcaactaat aatttatccg ctaatggatc caaaaatttc
     1621 cccatatttg taactaaatt ccattttcta gctaaataac catcaacaaa atcgctaagg
     1681 gaagccaata taaaaataaa accactgatt aataactcaa ttcttatttc atatcctcct
     1741 agaaatgaca cattgccaaa tccaaaatca actaacgcaa acaatataaa aactggtatt
     1801 aacactactc taaaaaccgt aatctggttc ggaatattca t
//
LOCUS       DAMQYT010000292         1830 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.300, whole genome shotgun sequence.
ACCESSION   DAMQYT010000292 DAMQYT010000000
VERSION     DAMQYT010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.300"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..1671
                     /gene="ezrA"
                     /locus_tag="PGA33_002684"
     CDS             <1..1671
                     /gene="ezrA"
                     /locus_tag="PGA33_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDG6474421.1"
                     /translation="IIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETLPFDQNLAQ
                     LSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNASQSEIDDA
                     NELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANRHQFGEAAS
                     LLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIPELIRETQK
                     ELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLELEEANDKL
                     ANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIEYVRENYYI
                     NESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHVTVINDKQE
                     KLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMKNEIDHEVR
                     DVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQYGNRYRKDY
                     SNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
BASE COUNT          735 a          223 c          341 g          531 t
ORIGIN      
        1 ataattgtga ttatattgat tgctgtaggt gtattattct atttacgttc aaataaacga
       61 caaattattg aaaaagcaat cgaacgtaaa aatgaaattg agacgttacc ttttgatcaa
      121 aaccttgcac aattatctaa gttgaattta aaaggtgaaa caaaaacgaa atacgatgca
      181 atgaaaaagg acaacgtaga aagtacaaat aagtatctag ctcctgtgga agaaaaaatc
      241 cataatgctg aggctttatt agataaattt agtttcaacg catctcaaag tgaaattgat
      301 gatgcaaatg agttgatgga tagttacgaa caaagctatc agcaacaatt agaagatgta
      361 aatgaaatta ttgcgttata caaagataat gatgaattat atgacaaatg taaggttgat
      421 tatcgtgaaa tgaaacgtga tgttttagca aatcgtcatc aatttggtga ggcagcaagt
      481 ctacttgaaa ctgaaattga aaaatttgag ccaaggttag agcaatatga agtactaaaa
      541 gctgatggta attatgtaca agcgcacaac catatagctg ccttgaatga acaaatgaaa
      601 cagctaagat cttatatgga agaaatacca gaattaatta gagaaactca aaaagaatta
      661 cctggtcaat tccaagattt aaaatatggt tgccgtgatc ttaaagttga agggtatgat
      721 ctggatcacg tgaaagtaga cagtacatta caaagcttaa aaacagagct tagtttcgtt
      781 gaaccattaa ttagccgctt agaattagaa gaagctaatg ataaactagc taatatcaat
      841 gataagttag atgacatgta tgatttaatt gaacatgaag ttaaagctaa aaatgatgtc
      901 gaagaaacaa aagatatcat tacggataac ttattcaaag ctaaagacat gaattataca
      961 ttgcaaacag aaattgaata tgtacgtgaa aactactata taaatgaatc tgatgctcag
     1021 agtgttcgtc aatttgaaaa tgaaattcaa agtttaattt ctgtatatga tgatatttta
     1081 aaagaaatgt ctaaatctgc tgtgcgatat agcgaggttc aggataattt acaatattta
     1141 gaagatcatg tcacagttat taatgacaaa caagaaaagc tacaaaatca tctgattcaa
     1201 ttgcgtgaag atgaagcaga agcagaagac aatctgctac gagtacaatc gaagaaagaa
     1261 gaagtgtatc gtcgattact tgcttctaac ttaacaagcg ttcctgaaag gtttatcatc
     1321 atgaaaaatg aaattgatca tgaagttcgt gatgttaacg aacaatttag tgaacgtcca
     1381 atacacgtta aacagttaaa agataaagtg tctaaaattg tgattcaaat gaatacattt
     1441 gaagatgaag caaatgatgt tcttgttaat gctgtttatg cagagaaatt aattcaatat
     1501 ggaaatagat atcgtaagga ctatagcaat gttgataaga gcttaaatga agctgaacga
     1561 ttatttaaaa ataatcgcta taagcgtgcg attgaaattg cagagcaagc tcttgaaagt
     1621 gttgagccag gtgttactaa acatattgaa gaagaagtta ttaagcaata gaaactagta
     1681 tgtagttata cttaaataat atgagcactc tgtcaaattg gactgatgag tttaataatt
     1741 gaagttagcc aacgatacgt tgtctagctt cttttttata tggataaatg aaagggacaa
     1801 taaatataaa tagcaattgt ttaaagataa
//
LOCUS       DAMQYT010000293         1825 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.301, whole genome shotgun sequence.
ACCESSION   DAMQYT010000293 DAMQYT010000000
VERSION     DAMQYT010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.301"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..453
                     /locus_tag="PGA33_002685"
     CDS             <1..453
                     /locus_tag="PGA33_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474422.1"
                     /translation="VTNRKKPFLFLKTLFMGVVFIFIGYISLAISAVIIYGIIQFITI
                     DFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVVLEHLIQWFLVYFAIYQAV
                     NEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIFNYRMRSYK"
     gene            712..1209
                     /gene="efb"
                     /locus_tag="PGA33_002686"
     CDS             712..1209
                     /gene="efb"
                     /locus_tag="PGA33_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HDG6474423.1"
                     /translation="MKNKLIAKSLLTLAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTD
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLK
                     QGLVK"
     gene            1363..1713
                     /gene="scb"
                     /locus_tag="PGA33_002687"
     CDS             1363..1713
                     /gene="scb"
                     /locus_tag="PGA33_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-B"
                     /protein_id="HDG6474424.1"
                     /translation="MKFKKYILTGTLALLLSSTGIATIEGNKADASSLDKYLTESQFH
                     DKRIAEELRTLLNKSNVYALAAGSLNPYYKRTIMMNEYRAKAALKKNDFVSMADAKVA
                     LEKIYKEIDEIINR"
BASE COUNT          707 a          216 c          297 g          605 t
ORIGIN      
        1 gttacaaata gaaagaagcc ttttctgttc ttaaagacac tctttatggg tgtggtattt
       61 atctttatag gatatatttc actggcaata tctgccgtaa ttatttatgg tattattcaa
      121 tttatcacaa ttgattttgg tagttttttc ttaatgggta ttatattgat cttgatttca
      181 agtatattcc aattatttat agttagatta ctttttagaa aaaagaatgt cgatttgaca
      241 gaggttgtcg ttttagagca tttaattcaa tggttcttag tttactttgc gatctatcaa
      301 gcagtaaatg aaaaaatgga cattaatgat attaatatcg acaatttcca atctgtcttt
      361 tttgacgtgt ctaatttgaa tttagtaatt ctaccaacgt taatcattag ctgggtcaca
      421 atatttaact atagaatgag aagttacaaa taaaatctat gagattatac cttcagacac
      481 caacattcaa atggtgtctt ttttgttgtg tggttttatt tttgaaattc gaaaaagtag
      541 aggcatgaat tttttgacta gtgtataagt gctgatgagt cacaagatag atagctatat
      601 tttgtctata ttataaagtg tttatagtta attaataatt agttaatttc aaaagttgta
      661 taaataggat aacttaataa atgtaagata ataatttgga ggataattaa catgaaaaat
      721 aaattgatag caaaatcttt attaacatta gcggcaatag gtattactac aactacaatt
      781 gcgtcaacag cagatgcgag cgaaggatac ggtccaagag aaaagaaacc agtgagtatt
      841 aatcacaata tcgtagagta caatgatggt acttttaaat atcaatctag accaaaattt
      901 aactcaacac ctaaatatat taaattcaaa catgactata atattttaga atttaacgat
      961 ggtacattcg aatatggtgc acgtccacaa tttaataaac cagcagcgaa aactgatgca
     1021 actattaaaa aagaacaaaa attgattcaa gctcaaaatc ttgtgagaga atttgaaaaa
     1081 acacatactg tcagtgcaca cagaaaagca caaaaggcag tcaacttagt ttcgtttgaa
     1141 tacaaagtga agaaaatggt cttacaagag cgaattgata atgtattaaa acaaggatta
     1201 gttaaataaa acttcaatcg ttgctgttat ctggaaataa ttaattaaat gttatgttaa
     1261 tttttgttaa tgaaaaaagt aatctattta atgacaggtt aatgtaattg tcctgaaatt
     1321 gactatatac tcagtaagta tcaattttaa ggagagctta taatgaaatt taaaaaatat
     1381 atattaacag gaacattagc attactttta tcatcaactg ggatagcaac tatagaaggg
     1441 aataaagcag atgcaagtag tctggacaaa tatttaactg aaagtcagtt tcatgataaa
     1501 cgcatagcag aagaattaag aactttactt aacaaatcga atgtatatgc attagctgca
     1561 ggaagcttaa atccatatta taaacgtacg attatgatga atgaatatag agctaaagcg
     1621 gcacttaaga aaaatgattt cgtatcaatg gctgatgcta aagttgcatt agaaaaaata
     1681 tacaaagaaa ttgatgaaat tataaataga taataaataa aacaggttga gacaaaaaat
     1741 ggtcttaacc tgttttcaat ttgcatatgt gataaattct atatcaaaat gcttatgtat
     1801 aatgaatgac atttaaaagt agggg
//
LOCUS       DAMQYT010000294         1815 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.302, whole genome shotgun sequence.
ACCESSION   DAMQYT010000294 DAMQYT010000000
VERSION     DAMQYT010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.302"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(45..515)
                     /locus_tag="PGA33_002688"
     CDS             complement(45..515)
                     /locus_tag="PGA33_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HDG6474425.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(563..1780)
                     /locus_tag="PGA33_002689"
     CDS             complement(563..1780)
                     /locus_tag="PGA33_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HDG6474426.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDNSLLRKEQNDTTDKDKTSNENSDSTNNSDSSNQQQPATDQNSNQNQGGTQQ
                     APQASNNQNGVVN"
BASE COUNT          546 a          289 c          282 g          698 t
ORIGIN      
        1 gatgattgtt gtgaatcatc tttatagcta attaattatt aatgttatag tgcttctttt
       61 ctaataattg ctgtatattg ccaaaagatt cgcatttgag caatattcca attattcatt
      121 cccattgtaa atcctgaagg cttaaatata ttcacatcag ttacttctaa agctgctgca
      181 actaaatatt gtaatggaat gctaagtgat ttcattgttt cggtaatgcc attttcatca
      241 tatacccatg aaaatggtaa ctctgattcg aaaacatcaa cactattgaa aatttcaggt
      301 agggctaaaa tataacatgc accatctaca ataggattgt cgatactatt tttgtaataa
      361 attaggagtg cttcatagtt ttctcgatgc tcatgattga cgtaaaaaaa ttcacttcta
      421 aaatgagcca tatcttggct atgaatgatt tgttgttcta acatattaaa catgccattt
      481 atatttgcaa tcttttcgta tgtttttcta ctcatgtatg atgctccttt cataaatagt
      541 aaatgttaat cgtcatataa tattaattta caacaccatt ttggttattt gaagcttgtg
      601 gcgcttgttg tgtgccacct tgattttgat ttgagttttg atctgtagca ggttgttgtt
      661 gattgctgga atcactgtta ttagttgaat cactgttctc gttagatgtc ttatctttat
      721 ctgtcgtatc attttgttct tttctcaata aactattatc taaaggcgtt aatggtatta
      781 atgaaccata atgattaatg acacgttgat ctaagaaatc atttttatca ttaataggtg
      841 ataattctaa gtctttacga agtaagtttg catatttttg aatgctttca acacttggat
      901 gataatagta aataccattt aacatatcat ctttaccttt taattgcgca gttttaattt
      961 caacatcatt tgttaagtac atttttgcta aagctttaat ttcagagtta gttaaattat
     1021 gctttgcatt tttacctaca atttgaatca cgttatcaag tttatcaata gaatcaactt
     1081 cctgtgcttt ttggaataaa atcttaatta attccatttg acgttgtcca cgttttaagt
     1141 ctgaatcatg atgtctagtt ctagcaactg ctaaagcctc atcaccattt aatttttggt
     1201 accctttttt aattttaatc ttaccagtat catctgtgtt aggttcattt aagtcgtatg
     1261 gcacatcata gtatatgcca ccaagctcat ttacagcctc gacaaatgct ttcatattga
     1321 ctctcacata ataatcaaca ggtacattca tggtagcttc taccgaatcc attgcggcaa
     1381 ttggaccacc atatgcatgt gcatgggtaa tcttatcgta atagccaact ttaggaatgt
     1441 agctgatagt atcacgtgga atactaagca ttctaatttg atgttttgat tgattaaaag
     1501 tagttaaaat catagcgtct gatctagagt gttcagcatc ctgtcctttt tttcttcttc
     1561 catcgttatc atcgatacct aagaaaagaa tagagatagg ttgttcttcg ggattgactt
     1621 tattatctct taagttggat tgacgattag catttttgct gtcttgagaa gattcgaatg
     1681 catcttggga cgttttaaaa agtaacgtag cgaagactat tggaacaaca atgagaacca
     1741 atgctagaag gatcaaaaag tattttaaaa atttattcat gattgatgct cctatattaa
     1801 atttttgtta aatca
//
LOCUS       DAMQYT010000295         1794 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.303, whole genome shotgun sequence.
ACCESSION   DAMQYT010000295 DAMQYT010000000
VERSION     DAMQYT010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.303"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..618
                     /locus_tag="PGA33_002690"
     CDS             <1..618
                     /locus_tag="PGA33_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDG6474427.1"
                     /translation="EDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGF
                     EYRAQKEKYDNLYKFLKENEKQYQYTGFTKEAINKTQNVGYQNEYFYITYLSRNLKEY
                     RKYYEPLIHKNDKEFKEGMQRARKELNYTANTNTVATLFSTNDERNRKEKINNVIDLS
                     EKIERTKDMPIKNTITTQLGNKLIGTKKARFDDKKVVSFGAFEDE"
     gene            611..817
                     /locus_tag="PGA33_002691"
     CDS             611..817
                     /locus_tag="PGA33_002691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474428.1"
                     /translation="MNKINDRDLTELSSYRVYQDINKDNDFTVNEKRFKQADVFEDLY
                     REKLKDTNKLREYNYLQNETFKSA"
     gene            832..1746
                     /locus_tag="PGA33_002692"
     CDS             832..1746
                     /locus_tag="PGA33_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HDG6474429.1"
                     /translation="MLKKAKFILMATILLSGCSTTNNESNKETKSVPEEMDASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVIGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSKDKGDDMSTLVGAVLSGFEYRAQKEKYDKLYKFFK
                     DNEEKYQYTGFTKEAINKTQNSGYENEYFYISAIPYNLAEYRDYFEPLLNKSDSEFSK
                     ELSNVKKQLKDKSKVSVTTTLFSKKKNYTKKSNSENVIKMAEEIKKDKEIPNGIELSI
                     KFSDNKINTVKPNFNGESTSEYGVFDQE"
BASE COUNT          781 a          197 c          327 g          489 t
ORIGIN      
        1 gaagatcatg gcattgtatt taatgcaagt ctacctttgt acaaagatgc catccatcaa
       61 aaaggatcaa tgcgtagtaa tgacaacggt gatgatatga gtatgatggt gggtacagtg
      121 ctgagtggct ttgaatatcg agcgcaaaaa gaaaagtatg ataatttata taaattttta
      181 aaagaaaatg aaaagcaata tcaatataca ggttttacta aagaagcaat taacaagacg
      241 caaaacgtcg ggtatcaaaa tgaatatttt tatattacat atttatcaag aaatttaaaa
      301 gaatatagaa aatattacga accattgata cataaaaatg ataaagagtt taaagaaggt
      361 atgcaacgag ctagaaaaga gctaaactat actgctaata caaatactgt agcaacgttg
      421 tttagtacga atgatgaaag gaatagaaaa gaaaagataa ataatgtaat agatttatcc
      481 gagaaaattg aaagaacaaa agatatgcca atcaagaata ctataactac tcaattagga
      541 aataaactta ttggcacaaa aaaagctcgt tttgatgata agaaagtagt gtcgtttgga
      601 gcatttgaag atgaataaaa taaatgatag agatttaaca gaattgagta gctatagggt
      661 ttatcaagac atcaataaag ataatgactt tacagttaac gaaaaacgat ttaagcaggc
      721 agatgtattt gaagatttat atagagagaa actaaaagac acaaataaat taagagagta
      781 taattattta caaaatgaaa cttttaaaag cgcataaata ggtgatgaga tatgcttaaa
      841 aaagcaaaat ttatcttaat ggcaacgata ctactatcag gatgttcaac taccaataac
      901 gaatccaaca aagaaacaaa atctgtacca gaagaaatgg atgcttcaaa atatgtagga
      961 caaggattcc aaccacctgc agaaaaagat gcgattgaat ttgcaaagaa gcataaagat
     1021 aaaattgcta agcgaggcga acaatttttt atggataact tcggtctaaa agttaaagct
     1081 acaaatgtta taggtagtgg cgatggtgta gaagtattcg tgcattgtga tgaccacgac
     1141 atcgtattta atgcgagtat tccatttgat aaatcaatta ttgacagtga tagctcatta
     1201 agaagtaagg ataaaggtga tgatatgagt actttagttg gtgcagtact cagtgggttt
     1261 gaatatcgag cacaaaaaga aaaatatgat aaattatata aatttttcaa agataatgaa
     1321 gagaaatatc aatatacagg atttacaaaa gaagcaatta ataagacgca aaatagtggt
     1381 tatgaaaatg aatattttta tatttcggcc ataccttata atttagctga gtatagagac
     1441 tattttgaac cattgttaaa caaaagtgac agtgaatttt caaaagaatt gtcaaatgtt
     1501 aagaagcaat taaaagataa gtctaaagtt tcggtaacta ctactctatt tagtaaaaaa
     1561 aagaactata ctaaaaaaag taacagtgaa aatgtaataa aaatggcaga agaaataaaa
     1621 aaagataaag agataccaaa cggtatagag cttagtataa aattttcgga caataaaata
     1681 aatacggtta aaccaaattt taacggtgaa agcacttcag aatatggtgt gtttgatcaa
     1741 gaataaaatt aatgatgaaa atttaacgga gaatagtgta tattgagtag atca
//
LOCUS       DAMQYT010000296         1792 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.304, whole genome shotgun sequence.
ACCESSION   DAMQYT010000296 DAMQYT010000000
VERSION     DAMQYT010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.304"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(22..510)
                     /locus_tag="PGA33_002693"
     CDS             complement(22..510)
                     /locus_tag="PGA33_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDG6474430.1"
                     /translation="MAINIIEYNRSYKEELIEFILSIQKNEFNIKIDRDDQPDLENIE
                     HNYLNNGGQFWLAINNHQNIVGTIGLIRLDNNMSALKKMFVDKGYRNLKIGKKLLDKV
                     IMTCKEQNIDGIYLGTIDKFISAQYFYSNNGFREIKRGDLPSSFPKLDVDNRFYYRNL
                     KD"
     gene            complement(786..1487)
                     /gene="isaA"
                     /locus_tag="PGA33_002694"
     CDS             complement(786..1487)
                     /gene="isaA"
                     /locus_tag="PGA33_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HDG6474431.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
BASE COUNT          556 a          310 c          263 g          663 t
ORIGIN      
        1 atcaattatt attgcatcga attaatcttt taaatttctg taataaaacc tattatctac
       61 atctaattta ggaaaactac ttggtaaatc tcctcgctta atttcacgga agccattatt
      121 agaataaaaa tactgtgcac taataaattt atcaattgtt cctaaataaa taccatcgat
      181 attttgttct ttacatgtca ttataacttt atctaaaagt tttttaccta tttttaaatt
      241 cctataacct ttatcaacaa acattttttt aagtgcagac atattattat ctagtctaat
      301 caaacctata gtaccaacaa tattttgatg attgtttatt gcaagccaaa attgccctcc
      361 attattcaaa tagttatgtt cgatgttctc caaatcaggt tgatcatctc tatcaatttt
      421 tatattaaat tcattttttt gaatcgataa aataaactcg attagctctt ccttataaga
      481 cctattatat tcaattatgt ttatagccat ttttatctcc tttttcattt aatttaatta
      541 taaaatgtgc gtttagtttg tatctagtgt actcagtaca gcctcaaatg aagtttcatt
      601 ccacttggca cttaataaag acaagtattt tagcagtaat acaataaagt ccaataaatt
      661 tccctaactt caatatccac tttttaaaaa atgtattttt aattaataaa aaaactctcc
      721 ccaatttcta tgggaagagc tatatattta atgtctaaac attactttta tttattatga
      781 aggaattaga atccccaagc acctaaacct tgtgctttgt atgctttaac agctgcgttg
      841 atttgttggt caacagtgtt tgttggaccc caacctggca tagtttggaa taaacctgaa
      901 gcacctgatg ggttgtaagc atttacttga ccatttgatt cacgagcgat gattgcagcc
      961 catgtagaag ctgaaacacc agtacgttga gccatgattt gagctgctga tgaaccagta
     1021 gcacctgcag tattaccatt gcttaatctc actgaacttg aagtagttga agtgctgtag
     1081 ttatggtaag ttggagctga aacagcttca acgtttgagt tacttgattg tgcattgtag
     1141 cttactgatt gtacatttga accttggttg tatgaagtag tgtagtctgc acctgcaacg
     1201 tttgagaaac cagcagtttg accattagct gcttcatagc tccatgacca tgtagtacca
     1261 tttgaagtga agttatattg gaaaccatct tttacaaagt ggatgtcata tgcaccatct
     1321 ttgattggag ctgcatttaa ttgatcttgg tgattatgcg ctaagtcaac taagtgtgct
     1381 tgatcaacgt ttacttcagc agcgtgtgct tgatgtcctg tacctgctgc gtaacctgtt
     1441 acacctaatg ccactgctaa tgatgatgcc ataattgtct ttttcatagt aaaaaatcct
     1501 ccagtaataa ttgtaagttt atgtttttag taattatatt ttgaatttga atgtcgtagt
     1561 gcaagtttaa attgtctttt atttctttca acggtactca ctatatcaca aaaaaccagc
     1621 cagtaaatta cactttcttt acaaaacatt acaatatcaa gtgttatttg taatgttgaa
     1681 atatggctgt tttatactgt aatgtgaaat atgtgccctt tagaatccaa tcaacccttg
     1741 aaatagtctt taacacataa gatttttact atatttagct caactattac ag
//
LOCUS       DAMQYT010000297         1786 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.305, whole genome shotgun sequence.
ACCESSION   DAMQYT010000297 DAMQYT010000000
VERSION     DAMQYT010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.305"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            76..1786
                     /gene="sasG"
                     /locus_tag="PGA33_002695"
                     /pseudo
     CDS             76..1786
                     /gene="sasG"
                     /locus_tag="PGA33_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
BASE COUNT          733 a          249 c          366 g          438 t
ORIGIN      
        1 ctcctattgt atttgtaaaa ttacatgtaa attcactgta agcaaagtgg aaaatatgga
       61 aaaaggagta tgcaaatgag agataagaaa ggaccggtaa ataaaagagt agattttcta
      121 tcaaataaat tgaataaata ttcaataaga aaatttacag ttggaacagc atctatttta
      181 attggctcac taatgtattt gggaactcaa caagaggcag aagcagctga aaacaatatt
      241 gagaatccaa ctacattaaa agataatgtc caatcaaaag aagtgaagat tgaagaagta
      301 acaaacaaag acactgcacc acagggtgta gaagctaaat ctgaagtaac ttcaaacaaa
      361 gacacaatcg aacatgaacc atcagtaaaa gctgaagata tatcaaaaaa ggaggataca
      421 ccaaaagaag tagctgatgt tgctgaagtt cagccgaaat cgtcagtcac tcataacgca
      481 gagacaccta aggttagaaa agctcgttct gttgatgaag gctcttttga tattacaaga
      541 gattctaaaa atgtagttga atctacccca attacaattc aaggtaaaga acattttgaa
      601 ggttacggaa gtgttgatat acaaaaaaaa ccaacagatt taggggtatc agaggtaacc
      661 aggtttaatg ttggtaatga aagtaatggt ttgataggag ctttacaatt aaaaaataaa
      721 atagatttta gtaaggattt caattttaaa gttagagtgg caaataacca tcaatcaaat
      781 accacaggtg ctgatggttg ggggttctta tttagtaaag gaaatgcaga agaatattta
      841 actaatggtg gaatccttgg ggataaaggt ctggtaaatt caggcggatt taaaattgat
      901 actggataca tttatacaag ttccatggac aaaactgaaa agcaagctgg acaaggttat
      961 agaggatacg gagcttttgt gaaaaatgac agttctggta attcacaaat ggttggagaa
     1021 aatattgata aatcaaaaac taatttttta aactatgcgg acaattcaac taatacatca
     1081 gatggaaagt ttcatgggca acgtttaaat gatgtcatct taacttatgt tgcttcaact
     1141 ggtaaaatga gagcagaata tgctggtaaa acttgggaga cttcaataac agatttaggt
     1201 ttatctaaaa atcaggcata taatttctta attacatcta gtcaaagatg gggccttaat
     1261 caagggataa atgcaaatgg ctggatgaga actgacttga aaggttcaga gtttactttt
     1321 acaccagaag cgccaaaaac aataacagaa ttagaaaaaa agttgaagag attccattca
     1381 agaaagaacg taaatttaat ccggatttag caccagggac agaaaaagta acaagagaag
     1441 gacaaaaagg tgagaagaca ataacgacac caacactaaa aaatccatta actggagtaa
     1501 ttattagtaa aggtgaacca aaagaagaga ttacaaaaga tccgattaat gaattaacag
     1561 aatacggacc tgaaacaata gcgccaggtc atcgagacga atttgatccg aagttaccaa
     1621 caggagagaa agaggaagtt ccaggtaaac caggaattaa gaatccagaa acaggagacg
     1681 tagttagacc gccggtcgat agcgtaacaa aatatggacc tgtaaaagga gactcgattg
     1741 tagaaaaaga agagattcca ttcgagaaag aacgtaaatt taatcc
//
LOCUS       DAMQYT010000298         1782 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.306, whole genome shotgun sequence.
ACCESSION   DAMQYT010000298 DAMQYT010000000
VERSION     DAMQYT010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.306"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            17..343
                     /locus_tag="PGA33_002696"
     CDS             17..343
                     /locus_tag="PGA33_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HDG6474432.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            345..824
                     /gene="rsbW"
                     /locus_tag="PGA33_002697"
     CDS             345..824
                     /gene="rsbW"
                     /locus_tag="PGA33_002697"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HDG6474433.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            799..1569
                     /gene="sigB"
                     /locus_tag="PGA33_002698"
     CDS             799..1569
                     /gene="sigB"
                     /locus_tag="PGA33_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HDG6474434.1"
                     /translation="MAKESKSANEISPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHQ"
BASE COUNT          667 a          218 c          358 g          539 t
ORIGIN      
        1 gtaggagtgt aatgaaatga atcttaatat agaaacaacc actcaagata aattttacga
       61 agttaaagtc ggtggagaat tagatgttta tactgtgcct gaattagaag aggttttaac
      121 acctatgaga caagatggaa ctcgtgatat ttatgttaat ttagaaaatg tgagttatat
      181 ggattcgaca ggtttaggtt tattcgtagg tacattaaaa gcattaaacc aaaatgataa
      241 agaactatac attttaggtg tgtcagatcg tatcggtaga ctatttgaaa ttactggtct
      301 taaggattta atgcatgtta atgaaggaac ggaggtcgaa taacatgcaa tctaaagaag
      361 attttatcga aatgcgcgtg ccagcatcgg cagagtatgt aagtttaatt cgtttaacac
      421 tttctggcgt tttttcgaga gctggtgcta catatgatga tattgaagat gccaagattg
      481 cagttagtga agctgtgaca aatgcagtta aacatgcata caaagaaaat aacaatgtgg
      541 gcattattaa catatatttt gaaattttag aagataaaat taaaattgtt atttctgata
      601 aaggtgacag ttttgattat gaaacaacta aatcaaaaat aggtccttac gataaagacg
      661 aaaatataga ctttttacgc gaaggtggcc taggtttatt tttaatcgaa tctttaatgg
      721 atgaagtcac agtatataaa gaatctggtg tgacaatcag tatgactaag tatataaaaa
      781 aagagcaggt gcgaaataat ggcgaaagag tcgaaatcag ctaatgaaat ttcacctgag
      841 caaattaacc aatggattaa agaacaccaa gaaaataaga atacagatgc acaggataag
      901 ttagttaaac attaccaaaa actaattgag tcattggcat ataaatattc taaaggacaa
      961 tcacatcacg aagatttagt tcaagttggt atggttggtt taataggtgc cataaataga
     1021 ttcgatatgt cctttgaacg gaagtttgaa gcctttttag tacctactgt aatcggtgaa
     1081 atcaaaagat atctacgaga taaaacttgg agtgtacatg ttccgagacg tattaaagaa
     1141 attgggccaa gaatcaaaaa agtgagcgat gaactaaccg ctgaattaga gcgttcacct
     1201 tctatcagtg aaatagctga tcgattagaa gtctcagaag aagaagtgtt agaagcaatg
     1261 gaaatgggac aaagttataa tgcgttaagt gttgatcatt ccattgaagc tgataaagat
     1321 ggttcaactg ttacgctatt agatattatg gggcaacaag atgaccatta tgacttaact
     1381 gaaaaacgta tgattttaga aaaaatatta cctatattat ctgatcgcga acgagaaatc
     1441 atacaatgta cgtttattga aggattgagt caaaaagaga caggtgagcg tatcggttta
     1501 agtcaaatgc atgtatcacg acttcagaga acggcaatta agaaattaca agaagcagca
     1561 catcaataga atttgtttat taatgatacg ttttataatg aaaaatccat ataattatcc
     1621 cttgattatt aaattgaaat cgaggggtat ttttaattta attaagattt tcgaattaat
     1681 acattattag tgtagtttaa tgtgtatcca cataaatgtc gcgatatagt attaataatt
     1741 taagtgaaga agatatctaa ttgtcgtttt aaataggtgg gt
//
LOCUS       DAMQYT010000299         1728 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.307, whole genome shotgun sequence.
ACCESSION   DAMQYT010000299 DAMQYT010000000
VERSION     DAMQYT010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.307"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(45..1526)
                     /gene="cls"
                     /locus_tag="PGA33_002699"
     CDS             complement(45..1526)
                     /gene="cls"
                     /locus_tag="PGA33_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDG6474435.1"
                     /translation="MRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
                     YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL"
     gene            complement(1584..>1728)
                     /locus_tag="PGA33_002700"
     CDS             complement(1584..>1728)
                     /locus_tag="PGA33_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474436.1"
                     /translation="KNINAFLSSEEATNLKLVNITIKEIEERTFPNNEEEFNAILTLS
                     VNK"
BASE COUNT          590 a          308 c          213 g          617 t
ORIGIN      
        1 caaaataaaa agagcctcta taatcgagac tccttacaaa taaattataa aattggcgaa
       61 actaattttg ctaacgattc tttgaattta acagacagcg gtctattggc atatgattct
      121 ttggttagtt gttttgattt tgtaatatca tgttcataag ccacccttaa atctttagca
      181 agattttcat catatacaaa ggcatttact tcaaaattta attcaaaact tctaaagtcc
      241 atatttgctg tgcccactga tacgatttca tcatcaatta agcacatttt agaatgtata
      301 aatccatttt catacgtata aattttaaca ccacttgata ataagtcaga ggcatttgaa
      361 aatgtcgccc aatatactaa tggatgatct ggcttacatg gaatcattaa atgtacatct
      421 acacctgatt tagcagcaat tttaatggca tttatatatg aattatccgg aatgaaatat
      481 ggtgattgta aatatacaga tttctttgca ctcataatca tttttgtata accgtattca
      541 atttgatgcc agtcactagc cgggccactt gcagctattt gaattggtga attgcccaat
      601 ggtccgttct ttttagggaa atacttaaca tcatattcaa attgtggacg gtgcgcttgc
      661 gaattccagt ctaaaataaa tcgcaactgc agtgcatcaa ccgcatcccc ttgtatacgt
      721 aaatgcgtat ctctccaata tcctaatttt cctaatccta gatattcatc accaatgtta
      781 aatcctccga cataacctag ttgaccatcg attacgatga tttttctatg atttctatta
      841 ttcattctga aattcaataa cggtaatttt gaagcaaaaa atgcttcaac ttctccacct
      901 aacgatttaa aatgatcaaa atttgccatc ttaacatttt tagatccaac atcatcatat
      961 aatattttta cttctagacc ttgtttcaat ttttcttcta aagcatgtaa aattctttta
     1021 cctaaaccat ctaaagcgaa agtatagtac tctaaatgga tatattcttt tgcattttta
     1081 atatctttta aaacttgatc atataaatca tttccatcaa tgaaatgatc aactttatta
     1141 ttttcagtta aaaaaccatc ttgatccatc aaaagcatac gtactaaatc atgatgtttt
     1201 tgaacttgtt tgttatcagt accataatta cctttatcaa agctttctat ttgttgtttt
     1261 aaaagtccat cgaaatccgt taacacgtta ccattgtttt tattcaattt gcgtgccgaa
     1321 acggttctac caaaaaacaa gtaaagaata aaaccaatca atggtaagac aaaaagtaca
     1381 aatagccatg cccaagttga actcgctgta cgcctatttc tttctaaaaa gataataata
     1441 aaagccaata ctaaattaat gatgaatcca atggctaaaa taatagtaaa taacgttcct
     1501 aaatcgtttg aaaatgtaaa tcgcatagtc ttttctccta taaagaaagg cacaaaaaaa
     1561 cattttgcac ctttcacgtc atattattta ttcacagata aagttaaaat tgcattgaat
     1621 tcttcttcat tatttgggaa tgttctttct tctatttctt taatagtaat atttactaat
     1681 tttaaatttg tagcttcttc agaacttaaa aaagcattaa tgtttttt
//
LOCUS       DAMQYT010000300         1719 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.308, whole genome shotgun sequence.
ACCESSION   DAMQYT010000300 DAMQYT010000000
VERSION     DAMQYT010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.308"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..309)
                     /locus_tag="PGA33_002701"
     CDS             complement(<1..309)
                     /locus_tag="PGA33_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDG6474437.1"
                     /translation="MFKKAKLILIATLLLSGCSAMNNESKKDTNTETNTETKSVPEEM
                     EASKYVGQGFQPPAEKDAIEFVKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVE
                     V"
     gene            complement(366..1283)
                     /locus_tag="PGA33_002702"
     CDS             complement(366..1283)
                     /locus_tag="PGA33_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HDG6474438.1"
                     /translation="MFKRTKLILIATLLLSGCSTTNNESNKETKSVPEEMEASKYVGQ
                     GFQPPAEKDVVEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGKGVEVFVHCDDH
                     DIVFNASIPFDKSIIESDSSLRSEDKGDDMSTLVGTVLSGFEYRTQKEKYDNLYKFFK
                     DNEEKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIHKNDKEFKE
                     GMEQARKEVNYAANTDTVTTLFSTKENFTKDNTVDDVIELSDKLYNFKNKPEKSTITI
                     QIGKPTINTKKAFYDDNDPIEYGVYRKDE"
     gene            complement(1374..1559)
                     /locus_tag="PGA33_002703"
     CDS             complement(1374..1559)
                     /locus_tag="PGA33_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474439.1"
                     /translation="MFILNTQAHARLDQMAQQFEVVCNGLNENEGQAIQTMDQSASLI
                     RSNLIQVKEQLEKLAVY"
BASE COUNT          477 a          306 c          220 g          716 t
ORIGIN      
        1 aacttctaca ccatcatctt tacctacaac atttgtagct ttaactttta gtccaaagtt
       61 atctttaaag aattgttcac ctactttttc aaattcttta cgatgcttct tcacaaattc
      121 aatcgcatct ttttctgcag gtggttgaaa gccttgtcct acatattttg aagcttccat
      181 ttcttctggt actgattttg tttctgtatt tgtttctgta tttgtgtctt tttttgattc
      241 attattcatc gctgaacatc ctgataacag tagcgttgct attaagatta atttcgcctt
      301 tttaaacata gctcatcacc catttatgtg tttatataga atattaaaaa gcattttaaa
      361 aaaatttatt catctttacg atacactcca tattctattg gatcattatc atcataaaag
      421 gcttttttag tattaatagt aggctttcct atttgaattg taattgtaga tttttctggc
      481 ttatttttaa aattatatag tttatcactc agttcaatta catcatcaac tgtattgtct
      541 ttagtaaaat tttcctttgt actaaacaat gttgttactg tatctgtatt agcagcgtaa
      601 ttcacttctt ttctggcttg ttccattcct tctttaaatt ctttatcatt tttatgaatc
      661 aatggttcgt aatacttacg atattctttt aagcttcttg acgagtaagt gatataaaaa
      721 tattcatttt tatatccgac attttgcgtc ttgtttattg cttcttttgt aaagcctgta
      781 tattggtatt tctcttcatt atctttgaaa aatttatata aattgtcata cttttctttt
      841 tgtgttcgat attcaaagcc actcaacact gtaccaacta aagtactcat atcatcgcct
      901 ttgtcctcac ttcttaatga gctatcactc tcaattattg atttatcaaa tggaatactc
      961 gcattaaata cgatatcgtg gtcatcacaa tgcacgaata cttctacgcc ttttccacta
     1021 cctacaacat tggtagcttt aacttttaga ccgaaattat ccataaaaaa ttgttcgcct
     1081 cgtttggcaa ttttatcttt atgctttttc gcaaattcaa ccacatcttt ttctgcaggt
     1141 ggttggaagc cttgacctac atattttgaa gcttccattt cttctggcac tgattttgtt
     1201 tctttattag attcgttatt ggtagttgaa catcctgata gcagtaatgt tgctattaag
     1261 attagtttag ttcttttaaa catagctcat cacccattta tgtgtttgta aaacttttat
     1321 gtttgaaaaa gctacttatt ctcaatgaaa acaagtagca tttaataaat taattagtat
     1381 acagctagtt tttctaattg ttctttaact tgaattaagt ttgaccgtat tagagaggca
     1441 gattgatcca tcgtttgaat tgcttgtcct tcattttcgt tcaagccatt acaaacaact
     1501 tcaaactgtt gtgccatttg atcaagacgc gcatgagctt gtgtgtttaa aataaacata
     1561 tcgtcataat gtgatggtaa attgacattt cgtcgttgta cgcaaatgta taaaaccctt
     1621 gttataccaa gttctttatt atttttaacc ttttaaattt tccacaagtg tttgtcctta
     1681 tttaaataat ccgccctttt tttgccctga aaaaataac
//
LOCUS       DAMQYT010000301         1709 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.309, whole genome shotgun sequence.
ACCESSION   DAMQYT010000301 DAMQYT010000000
VERSION     DAMQYT010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.309"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            132..671
                     /locus_tag="PGA33_002704"
     CDS             132..671
                     /locus_tag="PGA33_002704"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDG6474440.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQAA"
     gene            683..>1709
                     /locus_tag="PGA33_002705"
     CDS             683..>1709
                     /locus_tag="PGA33_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDG6474441.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNI"
BASE COUNT          630 a          196 c          291 g          592 t
ORIGIN      
        1 cacgtatgaa atgataaaag attttcaatt gaaataataa attttagaat tgtcgcataa
       61 ttcatcatga caacataatg acatgtattg ttattaacga tataatagaa ttgaataaag
      121 tgggtgataa tgtgactaaa aatgagatta gaaaatacat tttacataaa atgaagaatt
      181 ttaataaagc tgaaaagcga aaagcagaca catggttaag aaatcaattt tttgcaactg
      241 aagaatacaa agaagcaaac gcaattgcgc tagttctttc ttttaatcat gaagtagata
      301 ctttttctat tattgaacaa gccttaatgg atcataaacg tatttttgta ccgaaaatgg
      361 attatttaaa tcatcaaatg acttttaaag agatatttaa tctcaaagat attgatgtcg
      421 ataataaggg gatttactat ccaacttcaa aaggtgaaac aacgaataac ctagatttaa
      481 ttgttgttcc tggtgttgga tttcaagacg atggatatag aattgggtat ggtggtggct
      541 attacgacag gtttttagct aattatcaga caaagacaat aagcttatta tacgattttc
      601 aaataacatc atttgaaccg gaatcattcg atcaaccagt cgataaattg attatatatc
      661 aagcagcata gtggaggact aaatgaacat agacaaacaa ttttggaaaa caatatatta
      721 ttggattagg tacttgaatt ttgatattgt cagtagggaa aaagatgacc aagaaatttg
      781 gttagcccat aaaagaaaaa aacaagtagt catttttaaa caacatatca agtctaccca
      841 agaaatacgt tttgacaaag cgaaagtgct tgaacacaaa gatgaaatag caaattttat
      901 ttctttcgaa ccacaaagtt ttgaatttta ttattttaca gaatcggaat tttcagaaga
      961 acaattaaat gaagtttcgc caattagaat taaattcaat gttataagac acacaaaaga
     1021 tttgataaag catatgccga atatattttt ggctagactt atttcagaag ataatgataa
     1081 aaagacatat atgttctata aacgcaaagt attaaccgat aactttttag ataaatatat
     1141 gcagaaattt tcaccggcaa catacacaat aatatttgta aatgtcttaa tatggttatg
     1201 tatgatttta tatttaaata atttttcgga tgtaaaatta ttagatgttg gcgggttagt
     1261 gcattttaat gtcgtacatg gtgaatggta tcgaattgtt acatcgatgt ttttacattt
     1321 tagttttgaa catatactta tgaatatgct ttcattattt atttttggta aaatagtcga
     1381 agcaattatt ggttcatggc ggatgttaac tgtatacttt attgcagggt tgtttggaaa
     1441 ctttgtatca ctatcattta atacgactac aatttcagtt ggggctagtg gtgctatatt
     1501 tggtctgatt ggatcaattt ttgcgatgat gtatgtttca aaaacattta acaaaaaaat
     1561 gttaggacag ttattaattg cattagtgat attagttggt gtttctctgt ttatgtcaaa
     1621 tataaatatt gtggcgcata ttggaggatt cattggtggt ttattaataa ctttaattgg
     1681 ctattactat aaagtgaatc gtaatattt
//
LOCUS       DAMQYT010000302         1686 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.310, whole genome shotgun sequence.
ACCESSION   DAMQYT010000302 DAMQYT010000000
VERSION     DAMQYT010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.310"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..1301
                     /locus_tag="PGA33_002706"
     CDS             <1..1301
                     /locus_tag="PGA33_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDG6474442.1"
                     /translation="YSKRQLSKHLSISQTTVEHAYQLLLDEGYIYSRPRSGYFVSEIE
                     SLTILNNQPIPSLFDDDSYKPKASDEAYDYAFNLDEIDTKHFPIELFRKYSKDLYDTN
                     HLNQLRRGHFQGELHLRFQLAFYLFTNRGVICDPNQIIIGSSTEQLVNQLVDLLYTST
                     FIIEKPSYPPIKNILDKKQVEYEQIEVEDNGINVDEVIKSQKNIVYITPSHQFPTGYV
                     MDLKKRTQLIQWAQEKEERFIIEDDYDSEFRYFGKPIPAIQGLYSRGEKVIYISTFSK
                     SIFPSCRVAYMVLPYSIMKKYHSQNHIEGNTVPVHMQNLIATFISSGGFERHLNKMRR
                     IYRRKLTYILKRLKPYKEQLDIQGAETGMHFTITVKNGLTLQECLDRANKVKLKLQVY
                     NFDDDQDYKKTPKFILGFGGIDDDALKPHVDALIKSLIIK"
BASE COUNT          608 a          271 c          238 g          569 t
ORIGIN      
        1 tttattctaa aagacaatta agcaaacatt tatctattag ccaaacaacc gtagagcatg
       61 catatcaact tctattagac gaaggttaca tttattcaag acctcgctct ggatattttg
      121 tgtccgaaat tgaatcttta acaattttaa acaatcaacc tatcccctct ctttttgatg
      181 atgattcata taaaccaaaa gcgagtgatg aagcttatga ttatgctttt aatttggatg
      241 aaatagatac gaaacatttc cctatagaac ttttcagaaa gtattccaaa gatctttatg
      301 atacaaatca tttaaatcaa ttacgtagag gacattttca aggagaatta catttgcggt
      361 ttcaactggc attttattta tttactaatc gtggcgtaat ttgtgatcct aatcaaatta
      421 taattggctc atctaccgaa caattagtaa atcaattagt cgatttactt tatacttcaa
      481 catttattat cgaaaaacct agttatccac caatcaaaaa tattttagat aaaaaacaag
      541 ttgaatatga acaaattgaa gttgaagata atggaataaa cgtagatgaa gttattaaat
      601 cacaaaaaaa tatagtatat ataacgcctt cccatcaatt tccaactgga tatgtgatgg
      661 acttaaaaaa acgaactcaa ttaatacaat gggctcaaga aaaagaagaa cgatttatta
      721 tagaagatga ttatgattcc gagtttagat atttcggcaa acccattcca gcaattcaag
      781 gtctatattc aagaggagaa aaagtgattt atatcagtac attttcaaag tctatttttc
      841 ctagctgtcg agttgcatat atggtgttac cctattctat aatgaaaaaa tatcattctc
      901 aaaatcacat tgaaggcaac actgtacctg ttcatatgca aaaccttatt gcaacgttta
      961 tatcttccgg tggttttgaa aggcatttaa ataaaatgag acgaatttat cgtagaaaac
     1021 taacctatat tttaaaaaga ttgaaacctt acaaagagca gcttgatatt caaggtgctg
     1081 aaacgggtat gcattttaca attacagtaa aaaatggatt aacattacaa gagtgtcttg
     1141 atagagcaaa caaagtaaag ttaaaattac aagtttataa ttttgatgat gatcaagact
     1201 ataagaaaac ccctaaattt atattaggct ttggcggtat agacgatgat gctttaaaac
     1261 cgcatgtaga cgctctaata aaatcattaa ttataaagta acacttcttt gataaaagtt
     1321 agtgttactt atgcaatagc ttgtctattg tataataatt aatttccttt ttgtatttca
     1381 ataaagtata taaaagagca gtaagatcat tttcaattag ataccgtctt actgctcttc
     1441 tctatttata caatattttg ttttcgatga tatcatttta aatttatata aacacaaaat
     1501 taaagttgta catacttaga caatcgaaag tgtacattat taaattatca tttccagttc
     1561 tactctagcg gaacgtaagt tggctaccat cctcgctaag aacctttctt gacttgtgac
     1621 aatcgcttgc ttctttcctc tccttcggct ctcacttact catttagctc tactaaactc
     1681 gttgcg
//
LOCUS       DAMQYT010000303         1679 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.311, whole genome shotgun sequence.
ACCESSION   DAMQYT010000303 DAMQYT010000000
VERSION     DAMQYT010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.311"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(57..1466)
                     /locus_tag="PGA33_002707"
     CDS             complement(57..1466)
                     /locus_tag="PGA33_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDG6474443.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
BASE COUNT          628 a          284 c          272 g          495 t
ORIGIN      
        1 tattttcaaa aaaactctag tatcggttga actgatacta gagcgagatg tttaaattat
       61 tgattgtcat atctgaaatg accgctgtca ttttgtcgtt gttcatacgc gagcttttca
      121 gcattcgttt tgtatttttt ataaaagaaa aataaaaata taaaccagaa tggcgaaata
      181 taaatagctg ctcttgtttc gtcactaaag aataataaaa tgaatacaaa gaagaagaac
      241 gctagaataa tgtaagcaat aggcttacca ccaatcaact taaatttact gtttttatgt
      301 gcctcaggat gctttttcaa atacattaga taagcgacaa taatcattgc ccaaacaact
      361 aaaaacaaca cagttgataa cgttgtaacg tatatgaata gttgaattgc attagggaaa
      421 atgtagttta acaaggctgc tataagtaat gcaattgatg aaactaacat tgataaataa
      481 ggcacgccat gactattcgt tttatttaaa actttaggac ctaacccttg ttgtgacagt
      541 ccgaataaga tacgactatt cgaatagata ccactatttg tagcagaggc cgcggcagtt
      601 agcacgacaa agttaacgac acctgctgca aatggtacgc cgattaatgt aaagagttta
      661 acgaaagggc tactatttgg atcgatatca ttccaaggta tgactgacat aattactaat
      721 agaccaccga tatagaataa taaaatacgg ataggtacat tattaattgc tttcggtaag
      781 gttttttcag gatctttcgt ttcaccagca gttacaccta taagttcaat accaatgaat
      841 gaatatacag caatttggaa tgacattaag aaaccaaatg ttccaccagg gaacatgcca
      901 ccgtgactga ttaagtttgt gaatgatgca tgtccataat gtgttttaaa tgagaaaaag
      961 attaatacaa gaccaacgat aaccatcgca ataattgtga caactttaat aatcgagaac
     1021 caaaactcca gttcaccaaa taatctggca cctaataagt tgaagctaat caagattaaa
     1081 acaataaata gcacggtaat ccaatttggg acttgtggat accaaaatgc aaagtattgt
     1141 cccatcgcag ttaggtctga catacttgat acaatccaac ataaccagta agtccagcca
     1201 atgacaaagc caccaaaagg gcctaaatat tcatttgcaa tatcaacaaa tgaattaaat
     1261 cttgtattgc tcaacaacaa ttcgcctaat gcgcgcataa aagcgaatag tacaacccca
     1321 ataatcatgt atgtgaataa cagtgatgga ccagttaaag atattgtttg acctgatcct
     1381 aggaataacc cagttccaat tgcgccccca attgctatta attggatgtg tctgttattc
     1441 aactctctat gcaattttct tgccataatt ttcctcctca ggataaagca aataaaaaag
     1501 ttgtgtgtaa gcgttttctt aattataaat actaaagaaa tccatttaat aaagcaatta
     1561 tttttttaaa tataagaaaa aacatttgat aggtaagggt atgataacta aaacttatcg
     1621 attttttgaa aagatataag tttttatgca aaaatataaa ataatcatct aaatgaagc
//
LOCUS       DAMQYT010000304         1663 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.312, whole genome shotgun sequence.
ACCESSION   DAMQYT010000304 DAMQYT010000000
VERSION     DAMQYT010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.312"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            357..737
                     /locus_tag="PGA33_002708"
     CDS             357..737
                     /locus_tag="PGA33_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDG6474444.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            734..1630
                     /gene="pmtA"
                     /locus_tag="PGA33_002709"
     CDS             734..1630
                     /gene="pmtA"
                     /locus_tag="PGA33_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HDG6474445.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIIVLEEDMALNPIELKNRIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
BASE COUNT          602 a          203 c          311 g          547 t
ORIGIN      
        1 agccaaggta taaaattatt attagcagcg attatgttta tgatttgtgc ttttataagt
       61 agaagtaatg atcgacgtaa aaatagtaaa tagaattgac tgtttaaaac gattgtttat
      121 gttgacgcta cggttgtcac gtagagatta aagacattcg ttttagcagt cttttttatt
      181 gaagtagaca atgcaagatt aaatggtagt gtcatttcat tttattttat tcaactgcta
      241 gtaatacaat actcagtgtt atttctgaag actggttgat aatgagacat gcatatttac
      301 aaaactgtgt atattgtgta tattgtatat atacagaagt tgataggggg acgttgatga
      361 aaataatttt aaaaaacaat agtgattttc cgatttatga acagattaag caacaagtaa
      421 aacaaaatat tttaaaggga catgttgctc ctggagagca tttgccgtca atgagagaac
      481 ttgccaaaga tcttcaagta agtttgatta ctaccaaacg tgcttatgaa gatttagaga
      541 aagacggttt tgttacaaca attagaggaa aagggacctt tgttaaggag caagatagtt
      601 ctattttaaa agagaaacaa ttttttacca ttgaaaattt ggttaaagaa ttggttaatg
      661 aagcgcaagc catcgaaatg tcacttgagg aactgcagga tattttaacg ttcatttatg
      721 aggaggaatc atcatgaatg ccatagaatt aagtaatgtt aattattcaa gtgatcagtt
      781 taatttaaag aatatctctt tcaaagttcc tcaaggattc gtcactggtt ttattggtag
      841 aaatggcgct ggtaaaacca caataataag gttaattatg gatttgtatc aaccacaaac
      901 tggtgttatt atagtattag aagaggatat ggctctcaat ccgatagagt taaagaatag
      961 aattgggttt gtttactctg aaaattattt taatgagaga tggactacga agcaacttga
     1021 aaaaatgatt gccccttttt atcgtaaatg ggaccatcaa gtatttgaat tttatcttga
     1081 aaagttcgat ttaccaatca ataaatcaat aaaaacattt tcgactggga tgaaaatgaa
     1141 attatcacta gcagttgcat tttcacatca tgctgaattg tatatttttg atgagcctac
     1201 ttcaggtctt gatcccttag ctagaaatga gctattggag ataattcagc aagaattaat
     1261 tgatgaaaat aaaacaatat tcatgtcgac acacattatc tcagatttag aaaaaatagc
     1321 tgattatatc attcacttaa gtgacggtga agttatctta aatggttcaa aagaacaact
     1381 gttacaaaga tatcaagttg tgagtggtgc tattgaggac cttgatgatg aattagcatc
     1441 tctacttatt tatgaagaac ataagcgtac gggatttata ggattaacag aacatgcgca
     1501 agtatttaaa gaaattttag gtcataaagt gaatatcact acaccttcta ttgagaactt
     1561 aatggtttat cttgaaaaga gaaaacctaa atatcatgaa aacatcaaat tgatggaaga
     1621 aggtttttaa atgaaggcat tgttaattag gaattttaaa tta
//
LOCUS       DAMQYT010000305         1606 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.313, whole genome shotgun sequence.
ACCESSION   DAMQYT010000305 DAMQYT010000000
VERSION     DAMQYT010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.313"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            3..569
                     /locus_tag="PGA33_002710"
     CDS             3..569
                     /locus_tag="PGA33_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HDG6474446.1"
                     /translation="MNTKFLGKTLVASALVLTTLGTGLHSSYLGLDTNKVVKTAKAEE
                     KMTDGQLWKKVKDSLHDSDIILSNEYETINVTYLLSNGYSSSVSAPGNDDGGHLTQSI
                     DFKGLKQIDLTKENVYDDFNKKLDAKNTWNSLTEKLKGLGLLQNGQKVSIYSSDSSSP
                     VSGKVGEGVTSGGENTLTKRFINKITID"
     gene            complement(1043..>1606)
                     /locus_tag="PGA33_002711"
     CDS             complement(1043..>1606)
                     /locus_tag="PGA33_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
                     /protein_id="HDG6474447.1"
                     /translation="QISLFTASFNAITSLLISINVYQVINFEENIGHFNHILGKANRL
                     NWLNASMIFTYTITAICILLASINLLWHSHDMKITLMFIGVSLFFNVIILLLLFIFSI
                     FIKDVMAIVVGVLMFIFNVYFGLEVLGDHSWFYLPITYATRYVYMFIEGSIPVTLTLA
                     IYIIITLLLAVLLFKGFNKWSGRTIKD"
BASE COUNT          621 a          268 c          211 g          506 t
ORIGIN      
        1 aaatgaatac aaaattttta ggtaaaacat tagtagcaag tgctttagta ttaacaacat
       61 taggaacagg cttacattct tcatacttag gattagatac aaataaagtt gttaaaacgg
      121 ctaaagcaga agaaaaaatg acagatggtc aattatggaa aaaagttaaa gattcattac
      181 atgattctga tattatttta agtaacgagt acgaaacaat caacgttact tatttattaa
      241 gcaatggata ttctagcagc gtttctgctc ctggaaatga tgatggaggc catctaacac
      301 aatctataga ctttaaagga ttaaaacaaa ttgatttaac taaggaaaat gtctacgatg
      361 actttaacaa aaagctagat gctaaaaata catggaattc tctaaccgag aagttaaaag
      421 gtcttggttt actacaaaat ggccaaaaag tatctattta ttcttcagat agctcttctc
      481 cagtctcggg taaagtggga gaaggagtaa ctagtggtgg cgaaaatact ctaactaaac
      541 gcttcatcaa caaaatcaca attgattaaa ccttaaactc aaatttcctt aattcataaa
      601 acattaataa ctaacccttt cttacaaacc acctagccca taatatatta aaacgtcatt
      661 tttatttcaa ttagcggaaa ataaaaaaac cattccgcat taactttggc aagttggaat
      721 ggttaaaaaa atattttaga agccagcgtt tggcttgctt agacgacatg ttcgcgcatg
      781 tcgtcttatt tttacattta tataataaca cgcaattcaa taaaatgtct aatcaataaa
      841 cctcaattac attcaaattc aaaaactttt cacctaatat tcatcgctta aataaacatt
      901 acgtttaatt aaacagacat tgtcaatcgc gtctaatacc cctgctgtcc gaaaacaaaa
      961 ttattttgta ttcatattta actgttctct ttcctccctt ttcaacgcaa ctaaatttct
     1021 tttaccgtta gagccccaat ctttaatctt taattgttct accgctccat ttattaaatc
     1081 ctttaaagag taaaactgct aatagcaacg tgataataat atagattgcc aatgttaatg
     1141 taactggtat actcccttcg ataaacatat aaacgtaacg tgtagcatat gtgattggta
     1201 aatagaacca cgaatgatct ccaagcactt ctaatccaaa ataaacgtta aaaataaaca
     1261 ttaaaactcc gacaacaata gccattacat ctttaatgaa aatactaaaa ataaaaagta
     1321 gcagtaatat aattacattg aaaaacaatg atacgcctat aaacataagt gttattttca
     1381 tatcatgtga atgccacaat aaattaattg atgctaatag aatacatatg gctgtaatag
     1441 tataagtaaa aatcattgat gcatttaacc aatttagcct attagctttt cctaaaatat
     1501 gattaaagtg accaatattt tcttcaaaat tgataacttg atagacgttt atagaaatta
     1561 atagcgatgt aattgcatta aaactcgctg taaacaaact tatttg
//
LOCUS       DAMQYT010000306         1542 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.314, whole genome shotgun sequence.
ACCESSION   DAMQYT010000306 DAMQYT010000000
VERSION     DAMQYT010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.314"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(59..856)
                     /gene="fdhD"
                     /locus_tag="PGA33_002712"
     CDS             complement(59..856)
                     /gene="fdhD"
                     /locus_tag="PGA33_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDG6474448.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPMQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            1146..>1542
                     /gene="modA"
                     /locus_tag="PGA33_002713"
     CDS             1146..>1542
                     /gene="modA"
                     /locus_tag="PGA33_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDG6474449.1"
                     /translation="MKLKRLFAVVIAMLLVLAGCSNSNDNNESKKDDADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYT"
BASE COUNT          552 a          234 c          247 g          509 t
ORIGIN      
        1 gtttaaattg ttgattcatg tagaatatcg ttcattatga cacactataa tgaatatgtt
       61 attgttcaga atcaatgata cgttctggat gactgtatat attaaagcca ccatttcgaa
      121 taaatccaac tgccgtaata tttaggtcat tagctaaggt tacagcaagc gttgtcggag
      181 ctgatttaga taaaatgacg ccaacaccaa tttttgcggc tttaattaaa atttctgatg
      241 aaatacgtcc actaaaaatt aatactttat ctcggacagt aatatgtcgc tgaatacaaa
      301 atccatataa tttatctaga gcgttatgtc taccaatgtc ttgtcgatgt acaaaaaatg
      361 tcaaaccatc gcttatagca gcattatgta agccacctgt ttcttggtaa atatgacttg
      421 cactttgtaa tcgagtcatc atgttaataa tttgcattgg agttaaagtg attttagaca
      481 tagatgtttt agcgatagca gcatcatttt gaaaataaaa ctcacgactc tttccgcaac
      541 aagatgcaat cattcgtttt gtggaatatt gaaagcgatc gcctaaatct ttattaagtt
      601 caacatgggc aaaaccttta ctatcatcaa tcagtacaga ttttaattca tctcgcttta
      661 aaatggcacc ttccgaagcc agaaatccaa tgactaactc ctcaaggttt gttggactgc
      721 atataacagt cgcaaattct tcaccattca ccataattgt aagtggaaat tctgtcacat
      781 attgatctgt tgtattgaat aattttccat cttcatatct aacaattggt tgacctaaag
      841 atacatcttt gttcattatc taaccccttt aattagctta aactttattt taaagcaatt
      901 tgcttaaaat tttaacatat ttgcttaagt ttgaaatttg attgataaaa attaatagcg
      961 agcaatctgt ttgatttaaa ttgaattcga gaatatacat actagggcat caattaataa
     1021 atatcaatct tatgcaaatt tgacaattgt ttgaatcaat atataaacag gcaacggttc
     1081 ttttcaaata taatagtaag tgtataatga aaatgtaaat attattaaaa atgggggttc
     1141 actcaatgaa attgaaacgt ttatttgctg ttgtgattgc aatgctttta gtattagctg
     1201 gttgctctaa ttctaacgat aataatgaaa gtaaaaaaga tgacgcagac aatggtaaga
     1261 aacaagagat tcaagttgca gcggcagcaa gtttaacaga tgtaaccaag aaattagctt
     1321 cagaatttaa aaaagagcat aaaaatgctg atattaaatt taactatggt ggatcagggg
     1381 cattaagaaa acaaattgaa tcaggcgcac ctgttgacgt atttatgtct gcaaatacta
     1441 aagatgtaga tgcattaaaa gacaagaata aagcgcatga tacatataaa tatgcgaaaa
     1501 atagtctagt attaattggt gataaagatt caaattacac tt
//
LOCUS       DAMQYT010000307         1541 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.315, whole genome shotgun sequence.
ACCESSION   DAMQYT010000307 DAMQYT010000000
VERSION     DAMQYT010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.315"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..1118
                     /locus_tag="PGA33_002714"
     CDS             <1..1118
                     /locus_tag="PGA33_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474450.1"
                     /translation="RIDNILKLDYDKKSEKELDDIVKDEVEKKNNKYMTQGYVFQKLA
                     ISVIGFIITLFMSIVFLSISFAKLIFSTFALFLFLFLVFSWIVSFIPGFELSVFSAFA
                     KTLGYIILSACMTFLFVIVGLCIKLANSFIEPDSQNAYFLNSIFIIVILFVMYKKRAQ
                     IINFVSRGNISFSPSAIGAGVMNRTQERFNKIRHEQAQNKKAKRENQRDEPAPPLQND
                     NDLRRRQQDKPMPLFINKDNQKNGNKRREQQESMNGNDVKSASVESNANNYSKQPQKA
                     SQQEHQVRETRQRKDIQRSPQVVNQPLNNENHSINRKEQKSVQTAYDTDVQKRQIQNA
                     TQNQQSRQSGNRNQPITRNSQSKDRLKEQKDINKHGK"
     gene            1108..>1541
                     /locus_tag="PGA33_002715"
     CDS             1108..>1541
                     /locus_tag="PGA33_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDG6474451.1"
                     /translation="MVNKKDIAKKVIGKTPIGVKLKLAKVIIILCVVAFFIFPVIIMF
                     LLAPDLNKGKDDAGCTVSGGNVSANGIDKFNENAKGGKLEGKGKEIQKIAEKNKVPVN
                     IFMAIIASESQWGKGENATRQNNPLSVMGSKSIHDSTYPTIE"
BASE COUNT          593 a          193 c          276 g          479 t
ORIGIN      
        1 accgtattga taacatttta aaacttgatt atgataaaaa atcagaaaaa gaattagatg
       61 atattgttaa agatgaggtt gaaaagaaaa ataataaata tatgacgcaa ggttatgtat
      121 ttcaaaagtt ggctatttca gtgattggtt ttattattac actttttatg tcgatagtat
      181 ttttatcaat aagttttgcg aagttgattt tttctacatt tgctttattt ttattcttat
      241 ttttagtatt cagttggata gtgagtttta taccaggatt tgaattatct gttttctctg
      301 cgtttgcaaa aacgttaggc tatattattt taagtgcttg tatgacattc ttatttgtga
      361 tagttggttt gtgtatcaag cttgcaaata gttttattga accggatagt caaaatgctt
      421 attttttaaa ttcaatattt ataattgtaa tattgtttgt aatgtataaa aaacgggctc
      481 aaattatcaa ttttgtaagt cgtggtaata ttagtttttc tcctagtgca attggtgctg
      541 gtgtgatgaa tcgaacacaa gaacgtttta ataagattcg tcatgaacaa gcgcaaaata
      601 agaaagctaa acgagaaaat caaagagatg aacccgcccc accacttcaa aatgataatg
      661 atttaagaag aagacaacag gataaaccaa tgcctttatt tataaataaa gataatcaaa
      721 aaaatggtaa taagcgcaga gaacaacaag agtcaatgaa tggtaacgat gtaaaatctg
      781 cttctgttga atcaaatgca aataattatt ctaaacaacc acaaaaagca tcacagcaag
      841 aacatcaagt acgagaaaca agacagcgaa aagatataca aaggtcgcca caagtggtta
      901 atcagccatt aaataatgag aatcattcaa taaatcgtaa agaacagaag tcagttcaaa
      961 ctgcttatga tactgatgtt caaaaacgac aaatacaaaa tgcgacacag aatcaacaaa
     1021 gtagacaatc tggaaatcgt aatcaaccta ttacgcgtaa ttctcagtca aaagaccgtt
     1081 taaaagagca aaaggatatc aataagcatg gtaaataaaa aagatattgc taaaaaagta
     1141 ataggtaaaa cgccaatagg cgttaaattg aaattagcga aagtcatcat cattttatgt
     1201 gtggtggctt tttttatatt tccagtaatt ataatgtttt tgttagcgcc tgatttaaat
     1261 aaaggtaaag atgatgctgg ttgtactgtt agtggcggta atgtttcggc aaatggtatt
     1321 gataagttta acgaaaatgc taaaggtgga aaattagagg gtaaaggaaa agaaatacag
     1381 aaaattgcag aaaaaaataa agtacctgtg aatattttta tggccattat tgcgagtgaa
     1441 tcacaatggg gtaaaggaga aaatgcgaca agacaaaata atcctttgtc tgttatgggt
     1501 tcaaaatcga ttcatgattc aacatatcca actattgaag a
//
LOCUS       DAMQYT010000308         1529 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.316, whole genome shotgun sequence.
ACCESSION   DAMQYT010000308 DAMQYT010000000
VERSION     DAMQYT010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.316"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..181)
                     /locus_tag="PGA33_002716"
     CDS             complement(<1..181)
                     /locus_tag="PGA33_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDG6474452.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSS"
     gene            complement(192..884)
                     /locus_tag="PGA33_002717"
     CDS             complement(192..884)
                     /locus_tag="PGA33_002717"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HDG6474453.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFAAAKIGYHRLFDIKPVNTRYGGRIIFVDDCIDLNHYISFL
                     ESTPKQVVYETV"
     gene            complement(895..>1529)
                     /locus_tag="PGA33_002718"
     CDS             complement(895..>1529)
                     /locus_tag="PGA33_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HDG6474454.1"
                     /translation="PNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSH
                     SFGIENYSNYQGVDILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYN
                     LYFIKRNIEKLINADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAE
                     NIHQTLLKHHVFTSYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
BASE COUNT          497 a          281 c          210 g          541 t
ORIGIN      
        1 ggctagataa taatccacct aaaaagtact ttttaattcc aaagaaagct aatatcaata
       61 atgctgccgg tgcagataat tgaaaatcta atcctggtat aatggacggt attttcaaaa
      121 ctgccaaaat ggttaaaatc gcagcaatga cactaatttg agtaatatct tttgatgtca
      181 tactaaaacc cctataccgt ttcataaaca acttgcttcg gtgtgctttc taaaaatgat
      241 atgtaatgat ttaaatcaat acaatcgtcc acaaatatta ttctgcctcc atatctcgta
      301 ttaactggtt taatatcaaa taatcgatgg taaccaattt tagcagcggc aaaataacct
      361 gtcgtatacg ttaagtcatc ggacacgcaa agttctcctt tgacatacgg atgcgcattg
      421 atacaactag caattgctaa ggcatcagtc actctttcat taagatcacc tttattattt
      481 atatcttcaa acgaaaaatg tgttgcccta atcccccttt gtccaaatga atctaaacgt
      541 ttaccagata tagcagatag aataatagct cctgtataaa ccgtttcatt tttaatatat
      601 gtcatccctt gatttagcgc ttgttcagtg acaccacatt cttgtgttaa atgttggaga
      661 tttgctttat catcctcaat aatttgtaat gcttttattt gttgaatcgg ttccatgatt
      721 ttttgtattt ttatattgag aaaatccggt tgcccatttt catgaaaaaa acctttatta
      781 taaaaccttt gtaccgtctg ttctattttg tcaaactcac aaatcgtttc agcaccacta
      841 atatgaacat cttgattgct agaacgcatt tttatactat acatgacgat cacctcaatc
      901 ttcttgatgc aaaatttcaa acaacctatc tatatcttgt tcagtatgaa aatacgacaa
      961 tgatattctt aacattggct tagtcacagt tggatacctt aaataacttg taaacacatg
     1021 atgctttaat aatgtttgat gaatgttctc agccgcttct atgtcatcaa actcaataaa
     1081 cttaatcggc gagtttgaac tattataatt aacattgagt gcttttaact tttggttaaa
     1141 atatttactc aaactattta atttagtgcg tctatcatca gcatttatta acttttcaat
     1201 gtttcttttt ataaaataca aattataaat tggcaaacta cttgagtaga tgagtggtct
     1261 accgtgatta attaacatat ccttcacatc atttgaactt aaaatcacac ccccgtatgc
     1321 accacatgct ttagataaac tagaagtgag tatatctaca ccttgataat tcgagtaatt
     1381 ctctattcca aaactatgtg aaacatcgag tatcagtgtt gcgttatatt tatgctttaa
     1441 tgagactaat tgaccaatat ccacaacgtc gccattcgtt gaaaatacac tatcagatat
     1501 gattattttt ggtatatttt gattagggt
//
LOCUS       DAMQYT010000309         1524 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.317, whole genome shotgun sequence.
ACCESSION   DAMQYT010000309 DAMQYT010000000
VERSION     DAMQYT010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.317"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(89..313)
                     /locus_tag="PGA33_002719"
     CDS             complement(89..313)
                     /locus_tag="PGA33_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDG6474455.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            complement(373..1452)
                     /locus_tag="PGA33_002720"
     CDS             complement(373..1452)
                     /locus_tag="PGA33_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDG6474456.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVHQQSAKVKQQTQIV"
BASE COUNT          487 a          317 c          210 g          510 t
ORIGIN      
        1 ctaaaacaaa aaagactact tactcacttt taaattagcg gaagtagcca tagaatctaa
       61 tatctatttt attgttaaaa tgataacgtt aaactttttg aattgtaatt gtccatgaag
      121 cattatcaat ttgttcatag tttgttacag gataaccatt ttctgcagcc caatttggaa
      181 tggcttccgt cgcttgcgtg caatcaaaat caatttttaa ttcatctcca gattgcaatg
      241 ttgccatttt cttttgcgct tcaattaacg gaaatggaca taccattcct actgtaccta
      301 attcgtgtat cataattatt tctccttttc caatcacttt tatttgatca acatcatttg
      361 cataataatc ttctatacta tttgcgtttg ctgtttaacc tttgcagatt gttggtgtac
      421 ttttttcatt ggacgaacaa agataaaatg actcattgtc catacaccaa ctatcatcga
      481 tgctagcgca atccatcctt gccaagtcat cgttgccgtt tcaaccaaac cgttaccgat
      541 agaacaacca ccagcaactg acgcaccaaa tcccatacat atgccaccaa tggcactgtt
      601 tcgtattgta atcttgtctg gcaatcgcca tttaaattct cttgatcctc tagctgcaat
      661 atatgaacca atgaaaattc ctagaactaa aaagacaccc caatcaataa atttagtttc
      721 acctgtaatc aaaaagtgta ctaaatttgc tgaaggcgtt gttataccta aaccgtcatt
      781 tcttccagtt gatgcactca ttggccaagc taagagtgcg ataagtccaa ttacaattgc
      841 tgcaataaat ggatggtatc gtttttcgaa aaggtaatat ctaatacctg tataacgttg
      901 ctttagtttt ggaacagcaa ctcgaacttt cttattattg agtgttctta caactagaaa
      961 aatggtgatt atagttaata taacgactaa tccccaaaac ggaatgccag ttgtttgaga
     1021 catatcacta tttacattcg ttggttgatt aattttatcc attactggct ttaaaatccc
     1081 tgttttagtg attgctgcag taacagcata taatactaat gcaatccaac taccaattag
     1141 cccttcacca gcgcgatacc aagtacctgt tgcacatcct ccagccaata ctattccaat
     1201 tccaaaaata aaagaaccta taattgttcc caatattgga aaactatgtg caggaatttg
     1261 taaaatatct gttgccgtca aaatcaataa tcctatactt tgaatagtaa tagcgattaa
     1321 taatgcatag aacatcttat tattcttttg cacatacata tctcgaaagc cacctgttaa
     1381 acaaaatctt gttctttgca tgacaaaacc taagagtccc ccgacaatta agccactaaa
     1441 aatcatccac ataatcttaa cctctcattt ccgataagtt tagtatgtat tataggtgat
     1501 gattttcttg ttttcaacct tata
//
LOCUS       DAMQYT010000310         1515 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.318, whole genome shotgun sequence.
ACCESSION   DAMQYT010000310 DAMQYT010000000
VERSION     DAMQYT010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.318"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            90..959
                     /locus_tag="PGA33_002721"
     CDS             90..959
                     /locus_tag="PGA33_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDG6474457.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKEVMSIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            980..>1515
                     /locus_tag="PGA33_002722"
     CDS             980..>1515
                     /locus_tag="PGA33_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDG6474458.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFV"
BASE COUNT          583 a          201 c          288 g          443 t
ORIGIN      
        1 caatataaat gttaatagaa aagtatgtgc gctgagtata tattcttatt taagaacaag
       61 attttaaatt tacgaaacga ggtaacataa tgataaaact aatagccact gatatggatg
      121 gcacgctact taatgcagca catgaaattt ctcaacctaa tattgatgcg attaaatacg
      181 ctcaagaaca agggataacg gttgttatcg cgacaggtcg agcattttat gaagcacaag
      241 caccagttgc tgacacagat ttaacagtac catatatttg tttgaatggt gctgaagtac
      301 gtgatgaaac tttcaatgta atgagcactt cacaccttaa taaatcgtta gtacacaaaa
      361 ttacaaatgt tttaaaagat gcaggtattt attatcaagt atacacgagt cgtgcgattt
      421 atactgaaga tccacaaaga gatttagaca tttacataga tattgctgag cgtgcaggtc
      481 aacatgcaaa cgttgagcgt attaaaaatg gtattcaaag acgcatagat aatggtacgt
      541 tgaaagttgt tgataattat gatgctattg aaaacatacc tggtgaatta attatgaaaa
      601 tattagcatt tgatggaaat ttagaaaaaa ttgacaaagc tagtaaaatt ttagctgaat
      661 ctccgaattt agctatatca tcatcttcga gaggaaatat agaaataacg cattcagatg
      721 cacaaaaagg tattgcgcta gaaacaattg ccgaaagatt agggattgaa atgaaagaag
      781 tcatgtcaat aggtgacaat ttaaatgact tatcaatgtt agagaaagtt ggctatccag
      841 ttgcgatgga aaatggtgca gaagaagtta aaaaaatagc gaaatatgtc acagatacga
      901 atgaaaatag tggtgttgga aaagctatta tgaaattatt acgtgaacaa caagtttaat
      961 aaaaaaagag gggtcaaata tgaaaggatt aattattatt ggcagtgcac aagtgaattc
     1021 acatacaagt gcactagcaa gatacttaac tgagcatttt aaaacacatg atattgaagc
     1081 ggaaatattc gatttagcag aaaaaccgtt aaatcaatta gatttttcag gaacaacacc
     1141 gtctattgat gaaatcaaac aaaatatgaa agatttaaaa gagaaagcaa tggcggcgga
     1201 ctttttaata ttaggaacgc caaactatca tggttcatat tctggaatat tgaaaaatgc
     1261 attagatcat ctaaatatgg attattttaa aatgaaacct gtaggcttaa taggaaatag
     1321 tggtggtatt gttagttcag agccattgtc acatttaaga gtaatcgtca gaagtttact
     1381 aggcattgct gtaccaactc aaatagcaac acatgattct gattttgcta aaaatgaaga
     1441 tggttcatat tacttaaatg atagtgaatt ccaattacga gcaagattat ttgtcgatca
     1501 aattgtatct tttgt
//
LOCUS       DAMQYT010000311         1500 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.319, whole genome shotgun sequence.
ACCESSION   DAMQYT010000311 DAMQYT010000000
VERSION     DAMQYT010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.319"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            63..1268
                     /locus_tag="PGA33_002723"
     CDS             63..1268
                     /locus_tag="PGA33_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDG6474459.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIR
                     NIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPN
                     EYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIAS
                     ILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKR
                     SISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTK
                     ALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDREL
                     TQKLENIPS"
     gene            complement(1311..>1500)
                     /locus_tag="PGA33_002724"
     CDS             complement(1311..>1500)
                     /locus_tag="PGA33_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474460.1"
                     /translation="TLGKWEPFLNDLKAVSPILDNHLDDIVKNMKPLIVQMIFEPSHY
                     DDMFISRKALTPVFSSVL"
BASE COUNT          636 a          227 c          223 g          414 t
ORIGIN      
        1 ccgactaccc ttattatttt ttaatatttt atagaacata cgttcttgca ggaggtataa
       61 acatgtggat tgaaaaattt aaaaacaaaa ataacgaaac taaatacaga tactacgaga
      121 agtataaaga tccatacaca gataaatgga agcgcgtaag tgttgtgttg aacaagaata
      181 caaaacaatc tcaaaaagaa gcaatgtttc gtttagaaga aaaaataaaa gaaaaactga
      241 acaacaagtc gtcaagcgaa ttaaaaactt tgacttttca cgcgctatta gatgaatggc
      301 ttgaatatca tataaaaaca tcaggttcaa agttgactac tcttaataat ataaaaataa
      361 gaattagaaa cattaaacga tacagctctg agaacttgct tttaaacaaa ctagatacaa
      421 aatatatgca gatatttatt aataaattat cagatatcta ttctcaaaat caagtaaccc
      481 gtcaactcgg agatatgaaa ggagctatta aatatgcagt taaattttac aattatccaa
      541 atgaatattt gttaactaat gtcaaaattc ctaaaagaag aaaaacaata gaggatatcg
      601 aaaaagatga atctaaaatg tacaactatt tagaaatgaa ccaagtccta cagatacgtg
      661 atcatatact aaatgataat aagttacaca agcgaaatcg cattttaatt gccagcatct
      721 tagaagtaca ggctttaact ggtatgcgca taggagaact acaagcactg caggaaaaag
      781 atatagattt attaaacaaa actatcaata taacaggtac aattcaccgc attaaatacg
      841 aggaaggatt cggatacaaa gacactacaa agactataag ttcaaaaaga agtatcagca
      901 tcaattctag aaccgtagaa atttttaaaa agataatact ggaaaacaaa atgttgaaaa
      961 gatggaattc gagctatgtt gacagagggt tcatattcac aacaaaaaaa gggaatcctt
     1021 tatgtaataa tcaaatcgcc ggtgtgctta agaaaactac aaaagcttta aatatgaata
     1081 agaaagttac cacgcacaca tttagacata cacacataac tttattagta gaaatgaatg
     1141 tttctttaaa agcaattatg aaaagggtag gacatgtaga tgaaaaaaca accattcgca
     1201 tatatactca tgtaactgaa aaaatggata gagaactaac tcaaaaactc gaaaacattc
     1261 caagttagct taaatccgcc ctttttttgc ccttatattt tttacaagct ttataaaacg
     1321 cttgagaaca ctggcgttaa agcttttctt gaaataaaca tatcatcata atgtgatggt
     1381 tcaaatatca tttgtacaat caaaggcttc atgttcttaa caatatcatc taaatggtta
     1441 tctaaaattg gtgacactgc ttttaaatca ttaagaaaag gctcccattt gcctaaagta
//
LOCUS       DAMQYT010000312         1490 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.320, whole genome shotgun sequence.
ACCESSION   DAMQYT010000312 DAMQYT010000000
VERSION     DAMQYT010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.320"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(79..282)
                     /locus_tag="PGA33_002725"
     CDS             complement(79..282)
                     /locus_tag="PGA33_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474461.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            complement(311..1120)
                     /locus_tag="PGA33_002726"
     CDS             complement(311..1120)
                     /locus_tag="PGA33_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDG6474462.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
BASE COUNT          460 a          251 c          208 g          571 t
ORIGIN      
        1 agtatgcgaa ttgtttatat tttaccaaat ttactaattg aaaatctcat attaggcttc
       61 atttcaataa ttatgcgttt aaacttcttt gaaatatgtt ttatagtaac cgccaactaa
      121 tccagaatta tctattattt gataatattc ttcagtttcg ttgataacat catattgttt
      181 gccaaccgtt aacatgtcag aaactgtaaa tttctttgca tctgtattaa tgacttctac
      241 tttcttgatt ggtgtacgtt ctttccaagt ttcatgtaac ataaattttg tcctcgctat
      301 cgtaaaaaaa ttatttttgt aaagtgaata taaacgataa tccactgttt tcagtaattc
      361 taattgattc aatatcatct tgctcaagcg tttgatatgg tatttccact tcatctaagc
      421 gcgatttcgc ttgattaaag aaatctgcat ctttcgtttc aaactcaact tgatcgactg
      481 cgccatattc cggcattgtc atttcggttt ctacaggcat taaatgtatt tcgccaccta
      541 gaccacccgt tccaactttg aatacttgga catgatagtc agcattgtcg aagggttggt
      601 attctgcaaa tacttcaagt ccgaatatat ttgttaaaat ttgacctgta atatctacat
      661 gattcacttt aagaatcact ggtcctaaac cttgcacttg atgtaacgga ttgaccgcac
      721 tctcaaaaga aggcatacct aaaccaacgc cataattatt ctcgtttgaa tatattgaga
      781 aaatatgacc attgttatct tcgagactga aatatttatt gccatttaat tctttaactg
      841 ttgtaaatgg aatatcctta ttcgataata tttccgcata ctcctctaaa cctgagtcag
      901 ttggtgtacg taatccaata cccacaaagt gagattcttc taattgctca cttggaactt
      961 gtataaattg aagtcttgtt cctgggctta tatctgcatc gccgaaacga attgagtttt
     1021 caagaagttc ttcataattt aatcctaata tgtcaaccat gaaatgtttt gtctgttcta
     1081 tatttgttgt acctaatgtt atacttctaa gtccacacat tatagccacc tctatcttta
     1141 tctttactat tatattataa cttattttaa tcataaatta tagaatatcg gtcagttgat
     1201 tttgattata gattaatatg tgaaaaatgt atttctttaa aagaactatt tcatttaaat
     1261 aggtaacata atattgttat ggttgctatt gagtgtgtca ttaatgaata tctgtatata
     1321 gcaattgaat atttcgtttt ttgtaggaca tgaaaaacat atatatgtcg tgtccataaa
     1381 gcgaatttac atcatgttca gatgatttta aattaactaa aggcgcttat agaattcata
     1441 tatttcatcg tttgttatta tctaaaatgc attcattagc atattgaaac
//
LOCUS       DAMQYT010000313         1485 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.321, whole genome shotgun sequence.
ACCESSION   DAMQYT010000313 DAMQYT010000000
VERSION     DAMQYT010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.321"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            33..1187
                     /locus_tag="PGA33_002727"
     CDS             33..1187
                     /locus_tag="PGA33_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDG6474463.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPLFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
BASE COUNT          532 a          237 c          219 g          497 t
ORIGIN      
        1 acaatatttg atttatattt gaggtgtcac aaatgaaact ttctttaaat tctaattcta
       61 aatatttaag agcaccaagt attcgccaat tttcaaatcg tatgaataat ttggatgatt
      121 gtgttaattt aactattggc caaccagact tcccgatgcc tgatgttgtg aaaaaggcat
      181 atattgatgc tattaacaat gacaagacat catactctca caataaaggg ttattagaaa
      241 ctcgcgaagc aattagtcaa tacttcaaaa atcgttatca tttttcctat gatcctgaag
      301 aaattattgt gacaaatgga gcaagtgaag caatagacac aacgttacgt tctatcatag
      361 agcctggaga tgaaattata ataccgggac caatttatgc aggctacata ccactcatcg
      421 aagtactagg tggtaaacca atttatattg atacaacagc aacacaattt aaaattacac
      481 ctgatgcatt agaaagtcat atttctccaa agacaaaagc tgtcttgtta aattatccga
      541 ctaatccaac tggcgtagtt ttaaaaagaa atgaagtttt aaatatcgta aatgtattaa
      601 aaaaatatcc gttatttatt attagcgatg agatttatgc tgaaaataca tttagtggta
      661 aacatgtatc cttcgctgaa tttgaagaca ttcgtgatca attaatatta attggtggtt
      721 taagtaaatc acactcagca accggaataa gaattggttt tctattagga cctcaatatt
      781 tgattgataa attaacattt atgcacgcct ataattgtat ttgtgccaat gttccagctc
      841 aaatagcatg tataacagca cttaacgaag gattagaagc tcctaaatat atgaacgaag
      901 cttatgtaga acgaagaaat tatttagtat ctgaattaac taaattaggt tttgagatta
      961 ccgctcaacc tgaaggcgcg ttttatattt tcccaagtat taaacatatc accgatgacg
     1021 atttcgaatt ttgtgtcgat ttacttgaat caacacattt agcaatcgtt ccaggctcat
     1081 cctttacaga attcggtaaa ggatttgtaa ggatttccta tgcttatgaa atggatgttt
     1141 taaaagaagg tatgaagaga cttgctaaat atttaaatac taaataaaaa tgatatgttg
     1201 aactggacgt cgtgaactaa attcatagta cgttcagttt tttatattct ataagtttta
     1261 aataatactc tatagagact gtaaatacta ccgagttata ttcctctcct ttaaacacct
     1321 cactcaaaat gattctatca aaaggtttta atatgctaac ttataattgt tcttcgaaac
     1381 ttaagcactc aaataatata tcaccattca tattaatcta cctttggcca aacaattatt
     1441 attcaaaagt tactatttat tattataaac aaatactttc cattc
//
LOCUS       DAMQYT010000314         1483 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.322, whole genome shotgun sequence.
ACCESSION   DAMQYT010000314 DAMQYT010000000
VERSION     DAMQYT010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.322"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..272
                     /locus_tag="PGA33_002728"
     CDS             <1..272
                     /locus_tag="PGA33_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDG6474464.1"
                     /translation="VYRMFDELRETFKEEELEPWTSCEFDFTRDGKLNVSFDYIDWVN
                     SEFGPMGREHYYMYKKFGIWPEKEYAINWVEKIKDYVKEQDEAEL"
     gene            283..783
                     /locus_tag="PGA33_002729"
     CDS             283..783
                     /locus_tag="PGA33_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HDG6474465.1"
                     /translation="MTFEGKLSQMYNEIANEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKPDSDELNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKEEGLEPWTSCEF
                     DFTRDGKLNVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKEIEQYIKE
                     QEEAEQ"
     gene            794..1294
                     /locus_tag="PGA33_002730"
     CDS             794..1294
                     /locus_tag="PGA33_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDG6474466.1"
                     /translation="MTFEEKISKLYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFF
                     NYTKPGSDDLNYYTDIPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEGLEPWTSCEF
                     DFTSEGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEYEMEEIKEIDQYIKE
                     QDEAEI"
     gene            1305..>1483
                     /locus_tag="PGA33_002731"
     CDS             1305..>1483
                     /locus_tag="PGA33_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDG6474467.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVDDEGGEVVF
                     NYTKPGSEDLNYYSD"
BASE COUNT          588 a          137 c          319 g          439 t
ORIGIN      
        1 aagtttatcg aatgtttgat gagttaagag aaacttttaa agaagaagag cttgaaccat
       61 ggacatcatg cgaatttgac tttacaagag atggcaaatt gaatgtatct tttgattata
      121 ttgattgggt gaattcagaa tttggaccaa tgggaagaga acattattat atgtataaaa
      181 aatttggaat ttggcctgaa aaagaatatg ccataaattg ggttgaaaaa ataaaagatt
      241 atgttaaaga gcaagatgaa gctgaactat aggggagata atatgacttt cgaaggaaaa
      301 ctaagtcaaa tgtacaacga gattgcgaat gagattagtg ggatgatacc ggtagaatgg
      361 gagcaagtat ttacaatagc ttatgtaact gatcaagctg gagaagtcat ctttaattat
      421 actaaaccag atagtgatga attaaattat tattcagaca tacctaaaga ttgcaatgtc
      481 tcaaaagata tttttaagaa ttcatggttt aaagtttatc gaatgtttga tgagttaaga
      541 gaaactttta aagaagaagg gcttgaacca tggacatcat gcgaatttga ctttacaaga
      601 gatggcaaat tgaatgtatc ttttgattat atagattgga taaatacaga gtttgatcaa
      661 ttgggccgtc aaaattatta tatgtacaaa aaatttgggg ttataccaga aatggaatat
      721 gaaatggaag aagttaaaga aatcgaacaa tatattaaag agcaagaaga agctgaacaa
      781 tagaggtgat aacatgactt tcgaagagaa aataagcaaa ttatataatg agattgcgaa
      841 tgagattagc agtatgatac cggtagagtg ggaaaaagta tatacaatgg cttatataga
      901 tgatggagga ggtgaagtat tctttaatta tactaaacca ggtagtgatg acttgaatta
      961 ttacaccgat atacctaagg agtataacat ctctgtgcaa gtatttgatg atttatggat
     1021 ggatttatat gatttgtttg aggaattaag agatttattt aaagaagaag gacttgaacc
     1081 atggacatca tgtgaatttg actttacaag cgaaggtaaa ttaaaagttt catttgatta
     1141 tatagattgg ataaatacag agtttgatca attaggccgt gaaaattatt atatgtataa
     1201 aaaatttggg gttttaccag aaatggaata tgaaatggaa gaaattaaag aaatcgatca
     1261 atatattaaa gagcaagatg aagctgaaat ataggggaga taacatgact ttcgaagaaa
     1321 agctaagtca aatgtacaat gaaattgcaa atgaaatcag tggaatgata ccagtagaat
     1381 gggaaaaagt atatacaatt gcctacgtag atgatgaagg tggagaggtt gtttttaatt
     1441 atactaaacc aggaagtgaa gatttgaatt attattcaga tat
//
LOCUS       DAMQYT010000315         1477 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.323, whole genome shotgun sequence.
ACCESSION   DAMQYT010000315 DAMQYT010000000
VERSION     DAMQYT010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.323"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..425
                     /gene="fmhC"
                     /locus_tag="PGA33_002732"
     CDS             <1..425
                     /gene="fmhC"
                     /locus_tag="PGA33_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HDG6474468.1"
                     /translation="SKKNKSKLNQLNMQLSSINNRISKTEELIFEDGPVLDLAAALFI
                     CTDDEVYYLSSGSNPKYNQYMGAYHLQWHMIKYAKSHNINRYNFYGITGVFSNEADDF
                     GVQQFKKGFNAHVEELIGDFIKPVRPILYKFAKLIYKV"
     gene            598..1470
                     /gene="dprA"
                     /locus_tag="PGA33_002733"
     CDS             598..1470
                     /gene="dprA"
                     /locus_tag="PGA33_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDG6474469.1"
                     /translation="MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDSEYPVLLKEIYQFPLLLF
                     YKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
BASE COUNT          568 a          188 c          225 g          496 t
ORIGIN      
        1 attctaaaaa aaataaatca aaattaaatc agttaaatat gcaattatct agtattaata
       61 atagaattag taaaaccgaa gaactaatat ttgaagatgg acctgttttg gatttagctg
      121 ctgctttatt tatatgtact gatgatgaag tttattatct atcaagtgga tcaaatccga
      181 aatataatca gtatatgggt gcatatcatc tacaatggca tatgataaaa tatgcaaaat
      241 cacataatat taataggtat aatttttatg gaataacagg cgtctttagt aatgaggcgg
      301 atgattttgg tgttcaacaa tttaaaaagg gttttaatgc acatgttgaa gaattaattg
      361 gtgatttcat caaaccagta agaccaattc tatataaatt tgcaaaactt atttataagg
      421 tttaattata aagtatgttg gaaattgaaa ttttaaattc tttccaacat acttttcact
      481 ttttaagtca gtatttttac tccttaaaat aacattacat cgacaatgtt tttgtattag
      541 aaatgtaaaa caaaaataga atatgatttt gtatgattac aaaaaggagt tgatttattg
      601 attaaactat ttttgcttaa gttatactgg gcacactttt cgactaaaca aattcatcaa
      661 tttttaatgg catatcctaa tgtaattaaa gaggagggaa gaaaaaaaga tagttattta
      721 tgtgaatggg tgaataggga agaaaatgtt catttattac gtaaatacta tgcttttata
      781 aaacttgatc ataacgatat tattaaagaa ctgcagaaat taaaagtaag ttacattaca
      841 tatatggatt ctgaataccc agtgctatta aaagaaatat atcaatttcc attacttctt
      901 ttctataaag ggaacatcaa attaataaat aatatgcatc atttggcagt agtaggtgca
      961 agagattcta caagttatac ccaacagtct ttagaatttt tattatcaaa tgataaaagc
     1021 aaatatttaa caattgtttc cggccttgct caaggagctg atgcaatggc acatcaaata
     1081 gctttaaaat acaatctccc tacaattgca gttttagcct ttggccatca aacacattat
     1141 cccaaaagta cattagcatt aagaaataaa atagaagaaa aaggtttagt tatatctgaa
     1201 tatccaccac atacaccaat cgctaaatat agatttcctg agcgcaatag aattatcagc
     1261 ggtttgtcaa aaggggtttt aattactgag gctaaggaac aaagtggcag tcacatcacg
     1321 atagattttg cattagagca aaatagaaat gtttatgttt tacctggatc tatgtttaat
     1381 cctatgacaa aaggtaattt attacgtatc caagaaggtg ctaaggtagt attaaacgct
     1441 aatgatatat ttgaagacta ctatatttaa aactaaa
//
LOCUS       DAMQYT010000316         1454 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.324, whole genome shotgun sequence.
ACCESSION   DAMQYT010000316 DAMQYT010000000
VERSION     DAMQYT010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.324"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..>1454
                     /locus_tag="PGA33_002734"
     CDS             <1..>1454
                     /locus_tag="PGA33_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDG6474470.1"
                     /translation="LFYPYLLVQGEAGIGKSHLLAHLSKKLRDENHIIYLFLGQFFTK
                     NEDPWHQILNDLEVTNSVDNFLRSISNKAKETKKRAFIIIDALNEGEGKRLWGNYFQS
                     FINHIKKYSNIALIFSIRTPFEDVILPKNAIQDNNIVVFQHEGFSKEENYNPIVSFCD
                     FYGLELPKLPILNPEFNNPLFLKLMCEYCVNKFKEFDQTISVAELFTNVLKTVNINLS
                     KEDKFDFDKNINVVQKVIKGLVELMNDSEFNQLNYEESYTVVNNIAKEYVQKSNRFLE
                     ALIDENILIKNTGYKGEMIIYFSYERMGDYFLSEYLLEKYRNVDKRDLVTKLQSDEKV
                     TRYFQKEDDLSYNRGLINELFIKLANEFNIELFEVFPQFKNNYNMIYSFINSLVWRKD
                     GSISKHTKCYISDNVIPYDAFRNNFLDVLLIKMPQKNHPLNIWALHKLLKQCNLGKRD
                     FLWTQYISINNEKVFEIINWLFSNYKKLDEETAE"
BASE COUNT          578 a          148 c          220 g          508 t
ORIGIN      
        1 cttgttttat ccttatttgc tagttcaagg tgaagctggc ataggaaaat cacacctttt
       61 agcgcatcta tctaaaaaac ttagagatga aaatcatatt atttatctgt ttttgggaca
      121 attttttact aaaaatgaag atccatggca tcaaatactt aatgatttag aagttacaaa
      181 ttctgttgat aattttttaa ggtcaataag taataaggcc aaagaaacaa aaaaaagagc
      241 ttttattatt attgatgcgc ttaatgaagg tgaaggtaaa aggttatggg gaaattattt
      301 tcaaagcttt atcaaccata taaagaagta ttctaatata gctttaatat tttctattag
      361 aacccctttt gaagatgtta tattacccaa aaacgcaata caagataaca atattgtagt
      421 atttcagcat gaaggtttta gtaaggaaga aaactataat ccaattgtat ctttttgtga
      481 tttttatgga ttagagctac ctaagttacc tatattaaat ccagaattca acaatccatt
      541 atttttaaaa ctaatgtgtg agtactgtgt aaataaattt aaggaatttg accaaactat
      601 aagtgttgca gagctattta caaatgttct taagacagta aatattaatt tatctaagga
      661 agataaattt gattttgata aaaatattaa tgttgtacaa aaagtaataa aaggattagt
      721 tgaattaatg aacgactcag aatttaatca actaaattat gaggaatcct atactgttgt
      781 taataatata gctaaagagt atgttcaaaa gtcaaataga tttttagaag cgctaataga
      841 tgaaaatata ttaattaaaa atactggcta taaaggtgaa atgattattt atttttcgta
      901 tgagcgtatg ggagattact ttttatcaga atacctttta gagaaatata gaaatgttga
      961 taaaagagat ttagtaacta aattacagtc agatgaaaaa gtgacaaggt attttcaaaa
     1021 ggaagacgat ttatcttata atagaggtct tattaatgaa ctttttatta aattagcaaa
     1081 tgaatttaat atagagctat ttgaagtatt tccacagttt aaaaacaatt ataacatgat
     1141 atattctttt ataaatagtt tagtatggag aaaagatggt agcataagta aacatactaa
     1201 atgctatata tccgataatg ttattcctta tgatgcattt agaaataatt ttttagatgt
     1261 cttattaata aaaatgcctc aaaaaaacca tccgttaaat atttgggctt tacataaatt
     1321 attgaaacaa tgcaatttag gaaaaagaga ttttttatgg actcaatata tatctataaa
     1381 taatgaaaag gtttttgaaa ttattaattg gctatttagt aactataaga aattagatga
     1441 agaaactgcg gaaa
//
LOCUS       DAMQYT010000317         1451 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.325, whole genome shotgun sequence.
ACCESSION   DAMQYT010000317 DAMQYT010000000
VERSION     DAMQYT010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.325"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            104..370
                     /gene="zapA"
                     /locus_tag="PGA33_002735"
     CDS             104..370
                     /gene="zapA"
                     /locus_tag="PGA33_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDG6474471.1"
                     /translation="MTQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
     gene            371..892
                     /locus_tag="PGA33_002736"
     CDS             371..892
                     /locus_tag="PGA33_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDG6474472.1"
                     /translation="MVIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            965..>1451
                     /locus_tag="PGA33_002737"
     CDS             965..>1451
                     /locus_tag="PGA33_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HDG6474473.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LN"
BASE COUNT          533 a          191 c          252 g          475 t
ORIGIN      
        1 taattgcaat ataacaatat tagtatcaaa ttcagacaaa ctatccagtc atattttata
       61 acgtgaattt agattttaaa aaaaggatca ggagaaatta taaatgacac agtttaaaaa
      121 caaggtaaat gtatcaatta atgatcagct ttttacaatt gttggggaag ataacccaga
      181 gcacatacga tatgtagcac atttagttga tgataaaata aaagaattag ggtataaagc
      241 agcaggttta gatacttcaa gaaaagcaat actaactgct gtgaatatta tgcatgaaaa
      301 agtactacta gaagaagaaa atcgacgttt gaaacaacaa attcacaaat tgcagcagcg
      361 tgagcaataa atggtcattg attttatcat aatcattttc tttgtgtatt ttgtcatcgt
      421 tggattcaga cgaggttttt ggttatctat gatacatttg agtgcaacga ttgtatcatt
      481 gtggattgcc agtcaatttt acaaatctat tgtagaaaga ttaattgtat ttattccata
      541 tcctaaaaca acagcattta atacaacttt tgcgtttcat tttaatcatc tacaaaatcg
      601 atttgaagcg attgtagctt ttttaatgat tacattgttt tgtaagttca ttttatatct
      661 aattatcgta acttttgata aaataatagc gtatcaaaac attcatattt tcagtcgtgc
      721 aatgggaatg atagttggtg tgtttatgac gataattgtc ttacacttta cgttatatct
      781 attggcatta tatcctaacg aagcattaca acatcagctt aaaatatcta ttgtgagtca
      841 ttcattgatt tttcacatcc catatttatc ggctttcacc attaatttat aaattatcat
      901 aagaggtcag gacaaaggta tatattaatg atacgtcctg gtcttctttt tatggagtgt
      961 tattatgaca aaaaaagatg ttatcaaact attagaacaa attgctactt atatggaatt
     1021 aaaaggggaa aatactttta aaatatcagc gtatcgaaaa gcagctcaaa gtcttgaatt
     1081 agatgaacga ccattagatg aaatatctga tgtaacggag ttaaaaggca ttggtaaagg
     1141 tgttgcagaa gtaatcaatg attaccgtga gaccggtgaa tctcagtatt tacagcaatt
     1201 acaggaagaa gttccggaag gtcttattcc acttttgaaa attcaaggac ttggaagcaa
     1261 gaaaattgct aagctatata aagagttgaa tattgttgat aaagcgtcac ttcaagttgc
     1321 ttgtgaaaat ggaaaagtta gtgaattaag cggatttgct aagaaaacgg aacaaaacat
     1381 attagaagct gtgaaacaac ttggtgctaa gaaagataga tatccaattg atcaaatgag
     1441 aagacttaat c
//
LOCUS       DAMQYT010000318         1431 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.326, whole genome shotgun sequence.
ACCESSION   DAMQYT010000318 DAMQYT010000000
VERSION     DAMQYT010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.326"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            25..1245
                     /locus_tag="PGA33_002738"
     CDS             25..1245
                     /locus_tag="PGA33_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDG6474474.1"
                     /translation="MGYRTLKSIFHEHNESKMKEEYIKRFNSLASFNTNINIIPMENG
                     KKVNDLEYPLFFMVTKNLSKKQELISINSRKIDRALNSLPYAAREQYFNDLLIDELQS
                     TNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQYKEIELNKKIKVDNVRDIRAIYDK
                     LVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHVGLQPETKIVEFIGEMLTFLKYFD
                     APQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKLLSDYYDNYTALTFSYVINRNKSK
                     YYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERILDELIPKMDATEKLTLYLTSHYK
                     QIDYEFLYLLSMDKLFGNKRNRLTLIDLENILGVGRVKINNTIKKYDNYLVKIKSRPT
                     IYEISDEFLNSIIK"
BASE COUNT          570 a          145 c          237 g          479 t
ORIGIN      
        1 ttaaatgagt agaggaggct gcaaatgggt tacagaactt taaaaagtat ttttcatgaa
       61 cacaatgaaa gtaaaatgaa ggaagagtat attaaaagat ttaattcttt agcttctttc
      121 aatactaata ttaatatcat acctatggaa aatggaaaaa aagttaatga tttggaatat
      181 cctctattct ttatggtgac taaaaatcta tcaaaaaaac aagaattaat atcaattaat
      241 agtagaaaaa ttgatagagc acttaattct ttaccatatg cagctagaga acaatatttt
      301 aatgatttat taatcgatga attacaaagt actaatgaaa ttgaaaatgt atttagtact
      361 aaacaagaga ttgcacatgc gttaaataac caagcatcag aatttcttaa gttcagaggc
      421 ctcgtggatc aatataaaga gatagaactt aataaaaaaa ttaaagttga taatgtaaga
      481 gacattagag cgatttatga taaattagtt tcaaatgaaa ttaacgaaca agataagtta
      541 gatggagagc tatttcgtaa aaattttgtc ggtgtgcatg atgggtcaac gaataaatat
      601 atacatgttg ggttacaacc tgaaaccaaa attgttgaat ttataggtga aatgctaaca
      661 tttttaaaat attttgatgc gcctcagccg ttcaaaatca tggctagtca ttatctgttt
      721 gaatatatac atccttttta tgatggcaat ggtagagttg gaagatttat aattgccaag
      781 ctcttgagtg attattatga taattatact gctttgacat tttcatatgt tataaataga
      841 aataaatcga aatattacaa ggcgtttatg actgcttcaa atcatttgaa ttgtggtgat
      901 ttgacggaat ttattgacac aatgcttgag ctattgatag caggacaaga aagaatactt
      961 gatgagttaa taccaaaaat ggatgcgact gaaaaattaa cgctttattt aactagtcat
     1021 tataaacaga tagattatga atttttgtat ctcctttcaa tggataaatt gtttggaaat
     1081 aaaagaaata gattaacatt aattgattta gaaaacatat tgggagtagg aagagtaaaa
     1141 attaataata caattaaaaa atatgataac tacttagtta aaattaaaag tagaccaaca
     1201 atttatgaga taagtgatga atttctaaat tcgattataa aataaatata atatgtgatc
     1261 agctatactt atagtatttg taagatcgcg taattgcttc attgaaaaaa tatatttgtt
     1321 gagagtggtg catgacaaat gtactgctct ttttgttgtg tatcagtatt gtgatgtttt
     1381 aatgagaata ttatatgaat cattatgaaa tttaataaaa ataaaagaaa t
//
LOCUS       DAMQYT010000319         1427 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.327, whole genome shotgun sequence.
ACCESSION   DAMQYT010000319 DAMQYT010000000
VERSION     DAMQYT010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.327"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(2..226)
                     /locus_tag="PGA33_002739"
     CDS             complement(2..226)
                     /locus_tag="PGA33_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474475.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
     gene            425..895
                     /gene="sigS"
                     /locus_tag="PGA33_002740"
     CDS             425..895
                     /gene="sigS"
                     /locus_tag="PGA33_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HDG6474476.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRL
                     QDIFKLLNQRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN"
     gene            1008..>1427
                     /locus_tag="PGA33_002741"
     CDS             1008..>1427
                     /locus_tag="PGA33_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HDG6474477.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINPKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHF"
BASE COUNT          518 a          244 c          159 g          506 t
ORIGIN      
        1 tttacttagt ttctttttta taatccatca taataaaact tgctgcattt gatctatttt
       61 tataatagta ctgatttaaa tcaatcgtgc cttcaatttg accatcacga tacatcattt
      121 cattagtgaa gttatttatc atcacgaaat cagcgcgatc tcttataaca tctaaatcgt
      181 cgtttcgtgc aaaaaaatgt tctaaattta aatgtgcgta atccattgtt acctcccgat
      241 tacaaatttt gttctatcta ctatttagca aagtaataat acatcatatc acattaaatt
      301 aattacttat gtcttatatt taacacttaa tttagcgatt acgcaaatga atgatttttc
      361 taaatatttt attctaactt tatccggaaa tatttgttat acgtataact tggagaatga
      421 ctacttgaaa tttaatgacg tatacaacaa acaccacaaa atcatacacc atcttttaaa
      481 aaaatataat attagctata attatgatga gtattatcaa ctactcttga taaaaatgtg
      541 gcaattgagt cagatatata aaccctcaag caagcaatct ttatcctctt ttttattcac
      601 tcgattaaat ttttacctta tcgatttatt cagacaacaa aatcaattaa aagatgtcat
      661 tttatgtgag aataattcac caacattaac tgaacaacca acatacttta atgaacatga
      721 ccttcgttta caagatatct tcaagctttt aaatcaaaga gaaagactat ggctcaaact
      781 ataccttgaa ggatacaagc aatttgaaat tgctgaaatc atgtcattat cgctttcaac
      841 gattaaatta attaagatgt ccgttaagcg taaatgccaa cataatttta attagtttat
      901 tgtgaaattt cggtcgatgt gttaatgatg ttgaaacacc ctaacatctt catataaatt
      961 tattttaacc attaaattaa caacttatta aacgaggagt actatatttg caagacaatt
     1021 ctactaaata tctactttat atccaaactg ctacttcaaa ccatctcgaa acaaattgtg
     1081 tctttttaca ttgcgattac attcttaaag ttccaattaa caaactcgtt tcatattatg
     1141 cgaaattgca tttatcatca caaagtgtgc taattgagac tgcaaaaaac atactaaata
     1201 ttaataaact ggttcctatt tacatcaacc caaaaactat actttttccg ttaaaacata
     1261 aacgtgcacc gatacaaatt tatatcaatg cacattatat tgttggtatg actgccatag
     1321 agaattcaac attaatacat tttcaagaag gcattcagct agaagttgat gagccatttt
     1381 ctctagtttc aaaaaaatgt catgaaagtt tagctttgaa gcacttc
//
LOCUS       DAMQYT010000320         1420 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.328, whole genome shotgun sequence.
ACCESSION   DAMQYT010000320 DAMQYT010000000
VERSION     DAMQYT010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.328"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..1288
                     /locus_tag="PGA33_002742"
     CDS             <1..1288
                     /locus_tag="PGA33_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDG6474478.1"
                     /translation="SNINLEKIRPPYKSNWYKKEYSNLNIDDYIKSLKNKLDSHLHFS
                     IDKIKNSMTTEYGRGTGAYGDFGRYVFGYAVRNWVKGFKSDQDLSNIALMRIFEMGYD
                     AKLHGEFDMWVNRYDNFNNSIERISKKYQWIAYYEILAKLVDKFPDVQYSGLWDDYIR
                     DIDPTLLLLEIDKESKILVPSPLPSHQSNEWVKNTKVFDETKLFLEIDIDNHRYICLS
                     SKFNFEKREKEIPFEDRDSCYFLAMGYFYNKEDSNEIIKGYENNYDRGINIPRAHSIY
                     LYEYYWSEAYKNYKEGYLTESDGKLCPAIYEYFWELDYSVKDKSISFYIPCKEIVDYF
                     SLIQTEEGVWKTKFGETICINSKLLEFDNECLLIKKESLLNFLNTKKLSIGWKIYLEK
                     ISLRDRQEWWYNVFYDDGKYNKKIIKNDMSKIRRNF"
BASE COUNT          579 a          141 c          226 g          474 t
ORIGIN      
        1 atctaatata aatttagaaa aaattaggcc accttataaa agtaattggt ataaaaaaga
       61 atattcgaat ttaaatatag acgattacat aaaatcttta aaaaacaaat tagatagcca
      121 tttacatttt tctatcgata aaattaaaaa ctcaatgaca acggaatatg gaagaggaac
      181 tggggcatat ggcgattttg gcagatatgt atttggttat gctgtacgaa actgggtaaa
      241 gggttttaaa agtgatcaag acttaagtaa tatagcatta atgaggattt ttgaaatggg
      301 ttatgatgca aaattacatg gtgaatttga tatgtgggtt aatcgttatg ataactttaa
      361 taattcaatt gaaagaatta gcaaaaaata tcaatggatt gcttactatg aaattttagc
      421 taaacttgta gataaatttc ctgatgttca atattctgga ctatgggatg attatattag
      481 agatattgat cctactttac tattacttga aattgataaa gaatcaaaaa tactagtacc
      541 ctcaccactt ccttcgcatc aatcgaatga atgggtaaaa aataccaaag tttttgatga
      601 aacaaaatta tttttagaaa tagatataga caatcataga tatatatgtt taagctcaaa
      661 gtttaatttt gaaaagagag agaaagaaat accatttgaa gatagagact catgttattt
      721 cttagcgatg ggatattttt ataataaaga agatagtaat gaaattatca aaggttatga
      781 aaataattat gatagaggta ttaatattcc tcgagcacat agtatatatt tatatgaata
      841 ttattggagc gaagcttata aaaattataa agagggatat ttaactgaat cagatggtaa
      901 attatgtcca gctatttatg aatatttttg ggagttagat tattctgtta aggacaaatc
      961 aataagtttt tatattccgt gtaaagaaat agtagattat ttttcattaa tacaaacgga
     1021 agaaggagta tggaaaacta aatttggtga aacaatatgt attaattcta aattactcga
     1081 atttgataat gaatgtttac ttattaaaaa agaaagttta ttaaactttc ttaatactaa
     1141 aaaattgtct attggatgga aaatatatct agaaaaaatt tctcttcgag atagacaaga
     1201 atggtggtac aatgtttttt atgacgatgg caaatataat aagaaaataa ttaagaatga
     1261 catgtcaaaa attaggcgta atttttagat ttatctatat atttatgcca ccgctcaaac
     1321 ttttataaaa agtaaaaaaa caatatttat attgaacttg tgacgtgaat ttctaaaaca
     1381 caaccaacaa tccacaagga aactagtaaa taagtttaaa
//
LOCUS       DAMQYT010000321         1399 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.329, whole genome shotgun sequence.
ACCESSION   DAMQYT010000321 DAMQYT010000000
VERSION     DAMQYT010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.329"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..511
                     /locus_tag="PGA33_002743"
     CDS             <1..511
                     /locus_tag="PGA33_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HDG6474479.1"
                     /translation="VLTTVGSGFHSSSNYNGINNVAKAEQTTDNELWKNVRDALKEAN
                     IIDKTDNENVKVTYKLANGGENSIEGTANLNELSNSNNKPINSDSVKSIDLSRKNPNG
                     DRFDANDAWKKLTDKLKEKQIVKDGDTVTIHSKDEKDPKISAKVGEVYNDNKRLMLFK
                     KDIDKITIN"
     gene            complement(746..>1399)
                     /locus_tag="PGA33_002744"
     CDS             complement(746..>1399)
                     /locus_tag="PGA33_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDG6474480.1"
                     /translation="YREPHSYLYFKNYSSDTSKTDSIVKMVATFSSVMSNRKNKPLKH
                     YINTHNGVPLWILVNYLTLGNVSKMYSNLDDDLRLEVAKDYKRKLERDYKTRVQITPS
                     DVDSILQQAHMFRNVCAHEERLYDYKIDRAKSRANIFANYNKIYDKEYVPTMNGSYVF
                     DLLISLCLFLNKHDYIKLMKNMDKLISNYSHSFYTITIDDLYTKMNFPDQTKILDML"
BASE COUNT          510 a          206 c          198 g          485 t
ORIGIN      
        1 agttttaaca acagtaggtt caggttttca ttcttcttca aattataatg gtattaataa
       61 cgttgcaaaa gctgagcaaa cgacagataa cgaattgtgg aaaaatgtaa gagacgcttt
      121 aaaagaagcg aatattatcg ataaaacaga taatgaaaat gtcaaagtga cttataaatt
      181 agcaaatggc ggtgaaaaca gtattgaagg taccgccaat ttaaatgaac ttagcaattc
      241 taataacaaa ccgatcaata gcgatagtgt aaaaagcatt gatttgtcaa ggaaaaatcc
      301 caatggagat agatttgacg caaatgatgc atggaaaaaa ttaactgata aattaaaaga
      361 aaagcagatt gtgaaagacg gtgatacagt aactattcat agtaaagacg aaaaagaccc
      421 taaaatctca gctaaagtcg gtgaagtcta taatgataac aaacgattga tgttgtttaa
      481 aaaggatata gataaaatca ctataaacta gttatagata aagacaggtt acttttttaa
      541 tgtaacttgt ctttttttaa gaggcagtat ctcaaatatt aatttataat ttctaaatct
      601 atatctatac tttatctatt tctaaatcta tatctatact ttatctattt ctatatacac
      661 actcatccct cactgcaaca cagggcgttt ctcagcgtct taaaataaaa aaacgccact
      721 cgtaagtgac attaaaaaat atcttttata acatatccag tatttttgtt tgatctggaa
      781 aattcatttt tgtatatagg tcatctatag taattgtata gaaagaatgt gaataattac
      841 ttattagttt atccatattt ttcatcaatt ttatgtaatc atgtttattc aaaaatagac
      901 atagtgaaat caacaaatcg aatacataac taccattcat tgtaggaacg tattctttat
      961 cgtatatttt gttataattg gcgaatatat tagctctact tttagccctg tctattttat
     1021 aatcatacaa tctttcttca tgcgcacaca cgttacgaaa catatgtgct tgttgtagta
     1081 tactgtctac atctgatgga gttatttgaa cacgtgtttt ataatctctt tccaatttcc
     1141 ttttatagtc tttagcaact tccaatcgaa gatcatcatc caaattggaa tacatttttg
     1201 aaacattacc taaagttaaa taattcacca atatccatag tggcactcca ttatgagtat
     1261 taatataatg ttttaatggt ttattttttc tattactcat aaccgagcta aatgtagcaa
     1321 ccattttcac gatgctatct gtcttacttg tgtcagatga ataattttta aagtataaat
     1381 atgaatgtgg ttctctata
//
LOCUS       DAMQYT010000322         1386 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.330, whole genome shotgun sequence.
ACCESSION   DAMQYT010000322 DAMQYT010000000
VERSION     DAMQYT010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.330"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..145)
                     /locus_tag="PGA33_002745"
     CDS             complement(<1..145)
                     /locus_tag="PGA33_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474481.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNEL"
     gene            194..931
                     /gene="sdpB"
                     /locus_tag="PGA33_002746"
     CDS             194..931
                     /gene="sdpB"
                     /locus_tag="PGA33_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HDG6474482.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
     gene            complement(1022..1381)
                     /locus_tag="PGA33_002747"
                     /pseudo
     CDS             complement(1022..1381)
                     /locus_tag="PGA33_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          457 a          177 c          229 g          523 t
ORIGIN      
        1 tcaattcatt agtaactatt tggttaaatt tataaatatg cgtttgaatg ttccaatctg
       61 tcattaaagt attatattta atcgtatcaa tgatttgaat caaattagta atttgttgtc
      121 tcatatactt agtttcgttt atcatgtaga agtacctcca aaaatcaatt attaaacagg
      181 aaaggatata acagtggaaa atgaaaaaaa gaaatacacg tttaaagata tagcatggag
      241 agatttgctt ataattccaa ttataggtgt tatattttat gcactcacaa ttgcagctta
      301 cataggacca attttgtttg gttattatga aatcgatagt aatctgatat ttgtagcgac
      361 tatcacacaa gctttagcat atacgattgg gattggcgca ttttatttat ttcatttaaa
      421 agtgatgcca tcaaggcttc gagctggttt tgcgtatatc aaaaaatatt ggcttagatt
      481 gtcaataacg tatattattg ctctcatctt aatttataca tatgaatata tgacacagtt
      541 tctacctaaa catttgcaat atagtgaaac agccaatgaa cttgagctaa ataaaatgtt
      601 tgaagtgcct gcatttttac ctgtagcatt cttattaatt gtgattgtag gtccaatagt
      661 tgaagaaatc gtatttagac atattttaat tggagaatta ggtaagaagt ttaattttat
      721 agctatgagt attgtttcgg tgttcttatt tgcatttatt catgtgactg atgcaaagtc
      781 gccgtttgag tttggtccat atctcatact atccatcata ttagttttca cttatttaaa
      841 atctggaaga aacctaggtt caacaattgc attacatata gcaaataatt ttgtttcttt
      901 cgttatatca gtaataagta tttatgcgta gataaagagg tcgggacatt aagtttttag
      961 gcaatgaaaa aagctgattt ctattaatta tttgatagaa atcagctttt ttgatatgca
     1021 ttttattatg aaactgaata aataaagatt caattagcgc atcagtatta ggattcactc
     1081 taaaaagatt aatagtttta taagaaggtg tttgatcttg agctaaccac atcattcgaa
     1141 tactgttatg aagtaatttt tctattctac ggccagaaaa ttcagattga gtatatgaat
     1201 atgaggtgat ttttaacatc atttttagat gataggatgt tgcgccacga tgatgtctga
     1261 attcatcgaa ttcgctatca gctatctttt taacaatttc attaacatat cgcgaaatat
     1321 cattttgagg aattctaaca gcagtttcta ttggtagtgt aagttgggca aagtgtttta
     1381 tttttt
//
LOCUS       DAMQYT010000323         1378 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.331, whole genome shotgun sequence.
ACCESSION   DAMQYT010000323 DAMQYT010000000
VERSION     DAMQYT010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.331"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..262
                     /gene="rpsN"
                     /locus_tag="PGA33_002748"
     CDS             <1..262
                     /gene="rpsN"
                     /locus_tag="PGA33_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDG6474483.1"
                     /translation="KSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSSPTR
                     LTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            419..>1378
                     /gene="guaC"
                     /locus_tag="PGA33_002749"
     CDS             419..>1378
                     /gene="guaC"
                     /locus_tag="PGA33_002749"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDG6474484.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKTHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIF"
BASE COUNT          517 a          178 c          266 g          417 t
ORIGIN      
        1 gaaatctaaa atagcaaaag agagaaaaag agaagagtta gtaaataaat attacgaatt
       61 acgtaaagag ttaaaagcaa aaggtgatta cgaagcgtta agaaaattac caagagattc
      121 atcacctaca cgtttaacta gaagatgtaa agtaactgga agacctagag gtgtattacg
      181 taaatttgaa atgtctcgta ttgcgtttag agaacatgcg cacaaaggac aaattccagg
      241 tgttaaaaaa tcaagttggt aaaatcaaat ttcgtacttt agcccattta caatatcaat
      301 aaaacaatgt acagtatata cgaatgctat aaactgaatg ttttctcata ttaataagga
      361 aacattcggt ttttaatttg catttaaaaa taattagttt ttaaaggggc tatttaaagt
      421 gaaaatattt gattacgaag atattcaatt aatacctaat aaatgcatag ttgaaagtag
      481 gtctgaatgt gatacaacta tccaatttgg tccgaaaaaa ttcaagctac ctgtagttcc
      541 tgcaaatatg caaacagtta tgaatgagaa attagcgaaa tggtttgctg aaaatgatta
      601 cttttatatc atgcatcgtt ttgatgaaga agcaagaata ccttttataa aacatatgca
      661 aaattcaggc ttatttgcat ctatttcagt tggtgtaaag aaagcggaat ttgattttat
      721 tgaaaagtta gctcaagaaa aattaatccc cgaatatatt acaatagata ttgcgcatgg
      781 tcactcagat tcagtgataa acatgattaa acatataaaa acccatatac ctgatagttt
      841 tgttattgct ggtaatgttg gtacgccaga aggtgttaga gaattagaaa atgctggtgc
      901 tgatgctacc aaagtcggta taggtcctgg tagagtttgt attacaaaga ttaaaacagg
      961 ttttggtact ggtggttggc agttagcggc attaaacata tgtagtaaag cagctcgtaa
     1021 acctttgatt gccgatggtg gtataagaac gcatggcgac attgctaaat caattagatt
     1081 tggtgcatca atggtcatga ttggttcatt atttgcggca cacgaagaat cacctggtga
     1141 aactgtagaa cttgatggta aacagtataa agaatatttt ggtagtgcat ctgaatttca
     1201 aaaaggcgaa cataaaaatg tagaaggtaa aaaaatgttt gtagaacata agggttcatt
     1261 aatggatacc ttaaaagaaa tgcaacaaga tttacaaagc tcaatttcat atgccggtgg
     1321 aaaagacttg aaatcattac gtactgtaga ttatgttatt gttagaaact ctattttc
//
LOCUS       DAMQYT010000324         1367 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.332, whole genome shotgun sequence.
ACCESSION   DAMQYT010000324 DAMQYT010000000
VERSION     DAMQYT010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.332"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..506)
                     /locus_tag="PGA33_002750"
     CDS             complement(<1..506)
                     /locus_tag="PGA33_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474485.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLS"
     gene            complement(520..1311)
                     /locus_tag="PGA33_002751"
     CDS             complement(520..1311)
                     /locus_tag="PGA33_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDG6474486.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQGDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
BASE COUNT          435 a          239 c          186 g          507 t
ORIGIN      
        1 tggcttaaaa atgcatgttc taaatacaaa aataataaat ctgtatattg tgttttagta
       61 cgatacactt taatttgaga ttccgtatat gatatacgtg tatctaagcg aagatcaatt
      121 aatttagcag tacttgggcg ctcaacttta atagaattta atacgcgcat atcttctaat
      181 aatttaaaaa gttgttgata aaatttaggg tgtttaaaag ttttatcttg tactacttca
      241 tttacaattt gtacatccat catatgataa ccgtaagcag ctaacataac atctgttttt
      301 aaaccagcca tttcgatatg gcttggtcga tgtgaccaaa accaactcgt aatcaaaaca
      361 ttttgaacag gattatagta agggaatttt tggattttaa cttgtgtttg ttcatttttt
      421 aataagagtc gagctaagtc ttgcaacatc attacttgct tggcatctaa ctgttcgtca
      481 ttaaatttta tgaaacgatc actcatgttt tatccctctt taaaagttta gttctacagc
      541 attatatatt gcttgttgtt cacgttcatc ttccaattta tcaataattg tacgtttaat
      601 tgcacgttca actggcatta cagtgattaa atcacacaag tctaataatg cacggatact
      661 agcggcttct tcagaaattt gtccctgctt agacatagta cgtaaatctt cattaaactt
      721 aataatttgt tcgatttgtt tatcaccttg taataaactt tgctctttaa tcacattttt
      781 taaaatgtcc ccatcaatat aatcaacgtg aataacaaca aagcgatttt ttagtgcttc
      841 attcattggc aaagtaccaa cataaccttc atttatcgct gctataacgt taaatcctgg
      901 tacagctttg attacttcac cagtgtatgg attcgtaatt tgacgacgat aatctaagac
      961 cccatttaat acaggcaatg tttcaggttt agccatattt atttcatcaa tatataaaat
     1021 atgcccctct ttcatagctt taataactgg accatctaca aagacaattt cttgttgacc
     1081 ttccgcattt gttttaattg ttttaaagcc taataagctt tctgtatcta aatcaacaga
     1141 acaattgact tgatgcatgg gtgtatcaac aacttcactt aatgtttctg ccaactttgt
     1201 tttccctgaa cctgttggac ctttaagtaa aatattttta tttaaatcaa ataatgcctt
     1261 cgcatcattg aaaactgttg aatctgaatt cttataatgt tttagtgcca ttttaaaacg
     1321 ttctccttta aataaattaa aaaactaaga catggaataa gttctcc
//
LOCUS       DAMQYT010000325         1355 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.333, whole genome shotgun sequence.
ACCESSION   DAMQYT010000325 DAMQYT010000000
VERSION     DAMQYT010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.333"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..1318
                     /locus_tag="PGA33_002752"
     rRNA            <1..1318
                     /locus_tag="PGA33_002752"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          302 c          399 g          296 t
ORIGIN      
        1 cactgccgag aaaagcctct agatagaaaa taggtgcccg taccgcaaac cgacacaggt
       61 agtcaagatg agaattctaa ggtgagcgag cgaactctcg ttaaggaact cggcaaaatg
      121 accccgtaac ttcgggagaa ggggtgctct ttagggttaa cgcccagaag agccgcagtg
      181 aataggccca agcgactgtt tatcaaaaac acaggtctct gctaaaccgt aaggtgatgt
      241 ataggggctg acgcctgccc ggtgctggaa ggttaagagg agtggttagc ttctgcgaag
      301 ctacgaatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      361 ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa cgatttgggc actgtctcaa
      421 cgagagactc ggtgaaatca tagtacctgt gaagatgcag gttacccgcg acaggacgga
      481 aagaccccgt ggagctttac tgtagcctga tattgaaatt cggcacagct tgtacaggat
      541 aggtaggagc ctttgaaacg tgagcgctag cttacgtgga ggcgctggtg ggatactacc
      601 ctagctgtgt tggctttcta acccgcacca cttatcgtgg tgggagacag tgtcaggcgg
      661 gcagtttgac tggggcggtc gcctcctaaa aggtaacgga ggcgctcaaa ggttccctca
      721 gaatggttgg aaatcattca tagagtgtaa aggcataagg gagcttgact gcgagaccta
      781 caagtcgagc agggtcgaaa gacggactta gtgatccggt ggttccgcat ggaagggcca
      841 tcgctcaacg gataaaagct accccgggga taacaggctt atctccccca agagttcaca
      901 tcgacgggga ggtttggcac ctcgatgtcg gctcatcgca tcctggggct gtagtcggtc
      961 ccaagggttg ggctgttcgc ccattaaagc ggtacgcgag ctgggttcag aacgtcgtga
     1021 gacagttcgg tccctatccg tcgtgggcgt aggaaatttg agaggagctg tccttagtac
     1081 gagaggaccg ggatggacat acctctggtg taccagttgt cgtgccaacg gcatagctgg
     1141 gtagctatgt gtggacggga taagtgctga aagcatctaa gcatgaagcc cccctcaaga
     1201 tgagatttcc caacttcggt tataagatcc ctcaaagatg atgaggttaa taggttcgag
     1261 gtggaagcat ggtgacatgt ggagctgacg aatactaatc gatcgaagac ttaatcaaaa
     1321 taaatgtttt gcgaagcaaa atcactttta cttac
//
LOCUS       DAMQYT010000326         1337 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.334, whole genome shotgun sequence.
ACCESSION   DAMQYT010000326 DAMQYT010000000
VERSION     DAMQYT010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.334"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(204..557)
                     /locus_tag="PGA33_002753"
     CDS             complement(204..557)
                     /locus_tag="PGA33_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDG6474487.1"
                     /translation="MISIILVMIGGGFGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
     gene            complement(554..919)
                     /gene="crcB"
                     /locus_tag="PGA33_002754"
     CDS             complement(554..919)
                     /gene="crcB"
                     /locus_tag="PGA33_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDG6474488.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGDLS"
     gene            complement(1175..1324)
                     /locus_tag="PGA33_002755"
     CDS             complement(1175..1324)
                     /locus_tag="PGA33_002755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474489.1"
                     /translation="MSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVR
                     TFGFI"
BASE COUNT          503 a          197 c          214 g          423 t
ORIGIN      
        1 ttttttaggg atttatgtcc cactcccttt attttcgact agtttggaga atttattgac
       61 attcacatta tttaaacggc aacaaagatt attgaaattt ggtaggcatt atatggtgct
      121 taaaaatttg catgaaaatt aaaaaatgct tcgttcagga aggtgtcgta atttacctat
      181 ttgctgaatg aagcatttta tttttaaata tgatagccaa tataacaagc tataaatcca
      241 atgatgaatt gtaaaagtga ataattgaga aaaaggttaa tattaaattt tggcgtcatc
      301 attagtgtaa gttctttggc taacgttgag aaagttgtta agccacctaa aaaaccggta
      361 acaaagaacg cagggaacca tgagattgaa attgataagc ctatagttaa tccaattaaa
      421 aaactaccaa ctagatttac tatcaatgtt gcgataggta actttgaagt aaatttatga
      481 ttaaaataat cagtaatggc acttctagca attgcgccaa aaccgccgcc aatcatgact
      541 aaaatgattg atatcatgat aaatcaccac ctagttttat accgacgtaa cataacaaaa
      601 taccaaagac ataacttgtt acagcatata gtagtaaagt tataaattgt tgatgatcaa
      661 acatatgtat taattctaat tgaaatgttg aaaaagtcgt taaagcacca agaaaaccag
      721 tcgtaatagc tttttttagg gtcggatggt ttgaaaaaaa tgcaattgtt aaggctgtta
      781 gcaatcccat tacaaaggca ccagtcaaat tggctatcag tgttccgatt ggaaaacctc
      841 cgtcagtatt cagaaaagaa atgaggtaac gtaataaagc gcctaaagca ccaccgataa
      901 aaatatatac atattgcatt tggttcacct cgaaaagaag tagtttgaat ttaaaaaaga
      961 ggttttggca acgcgacgac aaaaattgtc gatgcattat caaacctcat tatatgttat
     1021 atcttgttgt ataactatag cgattagatg catagttatg atttcgaaaa tctaatattt
     1081 tttatacgca acaacgttat caaattgttt tactcattat agcatgatac attgtattgt
     1141 tttgtattaa ggctacaatg acatattaac ttttttaaat aaaaccgaat gtacgaacaa
     1201 ttgaaaagat atgtactaaa ataacaatta gaataatcca aggcaaactt ttactcgcaa
     1261 ttctaatcca atctgcatca ggctttagtg atttaattga acgatctgca aaaattatag
     1321 acaaaattag tacaatt
//
LOCUS       DAMQYT010000327         1334 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.335, whole genome shotgun sequence.
ACCESSION   DAMQYT010000327 DAMQYT010000000
VERSION     DAMQYT010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.335"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            1..1334
                     /locus_tag="PGA33_002756"
                     /pseudo
     CDS             1..1334
                     /locus_tag="PGA33_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
BASE COUNT          567 a          148 c          200 g          419 t
ORIGIN      
        1 caaatgaatt aaaattaatt agtaaagttt atatacgtaa aaaagacaaa aaactattag
       61 attggcaatt attaataaag aatgtatacc tagatactga agaagatgac aatttatttt
      121 cagaatccgg tcatcatttt gatgcaatat tatttctcaa agaagataca acattacaaa
      181 ataatgtata tattatacct tttggacaag catatcatga tataaataat ttgattgatt
      241 atgacttcgg aattgatttt gcagaaagag caatcaaaaa tgaagacata gttaataaaa
      301 atgttaattt ttttcaacaa aacaggctta aagagattgt taattataga aggaatagtg
      361 tagattacgt tagaccttca gaatcttata tatcagtcca aggacatcca cagaatcctc
      421 aaatttttgg aaaaacaatg acttgtggta caagtatttc attgcgtgta ccgaatagaa
      481 agcagcaatt catagataaa attagtgtga taatcaaaga aataaacgct attattaatc
      541 ttcctcaaaa aattagtgaa tttcctagaa tagtaacttt aaaagacttg aataaaatag
      601 aagtattaga tactttattg ctaaaaaaac tatcgaattc ttcaactaca gaaaatatat
      661 ctatagatat atcaagattt ttagaactaa gtaatatgat actcttgtta gatgatatgc
      721 tcgacgtcaa tatatatata taaattcttt taaaaataat acattagaga catttgatgc
      781 gactgatcct gaagttgatt acatcactga aataggagat tacttattaa aatatgacgt
      841 taattctata aatgatgtca gaattgaagt gatagataat ttggggcatt caaataatat
      901 gctactaaaa acgatactac atgcagaagt tgaaatggaa gatggaaaga aatatttatt
      961 acaaaatggg aaatggggtt attttaatag agaatttttt gaccttttga atgatcattt
     1021 gaatgagata gaaattaggt ataacacact aactcccaca ggtttagtat ttaaagaagg
     1081 agaagaagga tatataaaag aaatagtagg aagattgcca gaagaatatt taatgttaca
     1141 taaaaaattc ataaaaccaa taaataagaa ttttatagta aaaggaaatg gaatagagtt
     1201 ggcagactta tacaatatta aaaacaaaga gcttttcacg attaaaagag gaattaatac
     1261 atctttatct ctttatagtc tagaacagaa tataatagca attaacgcct taaaatatcc
     1321 agaatcatat aatt
//
LOCUS       DAMQYT010000328         1329 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.336, whole genome shotgun sequence.
ACCESSION   DAMQYT010000328 DAMQYT010000000
VERSION     DAMQYT010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.336"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(169..>1329)
                     /locus_tag="PGA33_002757"
     CDS             complement(169..>1329)
                     /locus_tag="PGA33_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDG6474490.1"
                     /translation="FPGFEGEWEEKKLGNLTTKIGSGKTPKGGSENYTNKGIPFLRSQ
                     NIRNGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRTAINSIVETHANLN
                     QHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFKEIANLKIFTPTIF
                     EEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQELRFKDENGEEYPEWEN
                     KFIKDIFIFENNRRKPITSSLREKGLYPYYGATGIIDYVKDYLFNNEERLLIGEDGAK
                     WGQFETSSFIANGQYWVNNHAHVVKSNDHNLFFMNYYLNFKELRAFVTGNAPAKLTHA
                     NLCNINLKIPCLTEQDKVSALLKSIDNKMNNQMNRIELLKERKKGLLQKMFI"
BASE COUNT          393 a          235 c          157 g          544 t
ORIGIN      
        1 ccctcttagt ttaacaatgt ctattctcat agaatttgtc caactaaatg tagacgattc
       61 actaaacttc taaaaaaata aaccccaaat tataatagtc tcaatattaa tatattacaa
      121 tttattcata ggatttttat catttcaatt aataggttgt tatcagggtt aaataaacat
      181 tttttgtaat agtccttttt tacgttcttt taataactca attctattca tttgattatt
      241 cattttattg tctatagatt ttaacaatgc acttacttta tcttgttcag tgagacaagg
      301 tattttaaga tttatattgc ataagttcgc atgagttaat ttagctggtg cattacctgt
      361 gacaaatgct cgtagttctt taaaatttaa ataataattc ataaaaaaca aattatgatc
      421 attactttta actacatgcg catgattatt tacccagtat tgcccattag caataaagct
      481 actcgtctca aactgccccc attttgcacc atcttctcct attagtaatc gttcttcatt
      541 attgaataaa taatctttta cgtaatcaat aattccagtt gcaccatagt aagggtataa
      601 ccccttttct cttaatgaag aagtaattgg ttttcttcta ttattttcaa agataaagat
      661 gtcttttatg aatttgtttt cccactctgg atattcttca ccattctcat ctttgaatcg
      721 caattcttgt gtgaagattt tctgcatata gccttttttc tgttgttgaa gcaattcaag
      781 tttttgttct tctaattcaa tttgtcggtc gagtttgctg aagaacttgc ctattttttg
      841 ctgttcttca aatatagttg gggtgaagat ttttaaatta gcaatttctt tgaagtttag
      901 accttctcga ctacctccac tttgtgcaag gaaaattttc cttttacctt ttcttgataa
      961 tagatactgt ccaaaaaaat tataataata ctcttttttc aatctaataa tacatacatg
     1021 ttgatttaaa ttagcatgcg tttcaactat cgaattaatg gctgttctac ctattgatgc
     1081 tcctgtaata tttaaaagaa catcaccata gtacgttcta ctatttttca tctcatcatc
     1141 tatatcttta ctaatataaa ctaagtcatt aagatttaat ttaccatttc taatattttg
     1201 actccttaaa aatggtatgc ctttgtttgt atagttttca cttccacctt tgggagtctt
     1261 tccactacct attttggtag taagattccc taacttcttc tcttcccatt cgccttcaaa
     1321 tcctgggaa
//
LOCUS       DAMQYT010000329         1321 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.337, whole genome shotgun sequence.
ACCESSION   DAMQYT010000329 DAMQYT010000000
VERSION     DAMQYT010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.337"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(233..>1321)
                     /locus_tag="PGA33_002758"
     CDS             complement(233..>1321)
                     /locus_tag="PGA33_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HDG6474491.1"
                     /translation="SNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRIY
                     DPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPED
                     TIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSPY
                     DFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELLK
                     EAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDIS
                     AVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDAG
                     QQMDLDEFQAFYDELKPLADLYNAKKLK"
BASE COUNT          405 a          236 c          191 g          489 t
ORIGIN      
        1 ttttaattac tgctatcagt cataatcttt aataattgtg aaattgaaca agtgtcgaag
       61 tattataaca atcgttaagt gcagtatacc tttatttcta tacaaaaaat aatagccact
      121 ctaaaagtaa tatactctta taatggctgt aagtttaaaa ttgtcgattc aaagataata
      181 ttctacttca acatgacata ttagttaagt agtctatagt ttccttggaa tattatttta
      241 actttttagc gttatataaa tcagctaaag gctttaattc atcataaaat gcttggaatt
      301 catctaaatc catttgttga cccgcatcac taagtgcaac agatggatct ggatgcacct
      361 cagccataac tccatcagca ccaactgcta atgctgcttt cgcagttggt aacatgatat
      421 ctttacgacc tgtactatgc gtaacatcta ccatgactgg taagtgtgta ccttgtttta
      481 aaattggtac tgctgaaata tctaaagtgt tacgtgtcgc cttttcataa gttcggattc
      541 cacgttcaca taaaataatg ttttgattac cttgtgaagc aatgtattca gctgcataaa
      601 caaactcttc gattgtagca gataaaccac gttttaatag aataggcttt ttcgtacggc
      661 cagcttcttt taataactcg aagttttgca tattacgtgc accaatttgg aatacgtcta
      721 aatactcatc agccacttca aaatcatttg gatttacgat ttcgctgaca acatttaaat
      781 catatttatc tttaatctgt ttaagtattt taagtccttc aacacctagg ccttggaaat
      841 catatggtga tgtacgtggt ttaaatgcac cgccacgaat aaatttttca cctttagcat
      901 gtaagttttt agcaacagct tcaacttgtt caaatgattc aactgaacat ggcccaaata
      961 caaatgattt attgccgtct ccaataatgc ccccattatc aaatgttaca atcgtatctt
     1021 caggtttcaa cttacgtgat acatataaat gtttttcatt ttcagatttt tgtaaatctg
     1081 tagaggcttt gaaaatttct ttaaataatt gcttaatagt attatcgttg aatggtcctt
     1141 tgttactatc gattaagtcg ttaagcattt ctttttcacg ttgtggatca taaatacgtg
     1201 taccttgttt taatttttct tccccaattt tttgtgctag ttcaccacgt ttagataata
     1261 agtctaaaat ttgatgattc agtgatacaa tctcacttct gtatgattct aatttattac
     1321 t
//
LOCUS       DAMQYT010000330         1309 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.338, whole genome shotgun sequence.
ACCESSION   DAMQYT010000330 DAMQYT010000000
VERSION     DAMQYT010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.338"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            265..1224
                     /gene="hyl"
                     /locus_tag="PGA33_002759"
     CDS             265..1224
                     /gene="hyl"
                     /locus_tag="PGA33_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HDG6474492.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWIDRSSERYKIDWEKEEMTN"
BASE COUNT          496 a          198 c          211 g          404 t
ORIGIN      
        1 tgtcttttcc ttgtttcata aagaatttat tcaactttga ctaaccctcg aaattgaaat
       61 gcttcctttc aaattttaaa taaaagttaa aaacatattc ttaaaataat cactcatcat
      121 cactcagtaa tttatcagtt gctacattaa atatactaat ataaacgatt ataaatattt
      181 gatatgtctc aactgcaata ttctaaattg acatattgat tattgtttcc tcaaatcgtt
      241 ttaaaaaata gaaggatgat gaaaatgaaa acacgtatag tcagctcagt aacaacaaca
      301 ctattgctag gttccatatt aatgaatcct gtcgctaatg ccgcagattc tgatattaat
      361 attaaaaccg gtactacaga tattggaagc aatactacag taaaaacagg tgatttagtc
      421 acttatgata aagaaaatgg catgcacaaa aaagtatttt atagttttat cgatgataaa
      481 aatcataata aaaaactgct agttattaga acgaaaggta ccattgctgg tcaatataga
      541 gtttatagcg aagaaggtgc taacaaaagt ggtttagcct ggccttcagc ctttaaggta
      601 cagttgcaac tacctgataa tgaagtagct caaatatctg attactatcc aagaaattcg
      661 attgatacaa aagagtatat gagtacttta acttatggat tcaacggtaa tgttactggt
      721 gatgatacag gaaaaattgg cggccttatt ggtgcaaatg tttcgattgg tcatacactg
      781 aaatatgttc aacctgattt caaaacaatt ttagagagcc caactgataa aaaagtaggc
      841 tggaaagtga tatttaacaa tatggtgaat caaaattggg gaccatatga tagagattct
      901 tggaacccgg tatatggcaa tcaacttttc atgaaaacta gaaatggctc tatgaaagca
      961 gcagataact tccttgatcc taacaaagca agttctctat tatcttcagg gttttcacca
     1021 gacttcgcta cagttattac tatggataga aaagcatcca aacaacaaac aaatatagat
     1081 gtaatatacg aacgagttcg tgatgactac caattgcact ggacttcaac aaattggaaa
     1141 ggtaccaata ctaaagataa atggatagat cgttcttcag aaagatataa aatcgattgg
     1201 gaaaaagaag aaatgacaaa ttaatgtaaa ttatttgttc atgtacaaat aaatataatt
     1261 tataacttta gccgataact tcagaaatga tgtttttcgg ctaattttt
//
LOCUS       DAMQYT010000331         1291 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.339, whole genome shotgun sequence.
ACCESSION   DAMQYT010000331 DAMQYT010000000
VERSION     DAMQYT010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.339"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..542)
                     /gene="agrB"
                     /locus_tag="PGA33_002760"
     CDS             complement(<1..542)
                     /gene="agrB"
                     /locus_tag="PGA33_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HDG6474493.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTIAYILNIFLFTLITNLTFYLIRRHAHGAHAPSSFWCYVESIILFILLPLVIVNF
                     HINFLIMIILTVISLGVISVYAPAATKKKPIPVRLIKRKKYYAIIVSLTLFIITLIIK
                     EPFAQFIQLGIIIEAITLLP"
     gene            778..912
                     /locus_tag="PGA33_002761"
     CDS             778..912
                     /locus_tag="PGA33_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HDG6474494.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
BASE COUNT          496 a          168 c          184 g          443 t
ORIGIN      
        1 ataggtaata atgtaatagc ttctattatg atgcctaatt gaatgaattg ggcaaatggc
       61 tctttgatga taagtgtgat aatgaaaagg gttaaactaa caataatcgc ataatatttt
      121 tttcgtttaa taagtcgcac aggaatgggc ttctttttag ttgctgcagg agcatatact
      181 gagattacac ctaaagaaat aactgttaaa ataatcataa ttaaaaagtt aatatgaaaa
      241 tttactatta ctaaaggtaa aagtataaat agtataatac tttctacata acaccaaaaa
      301 gaagaaggtg catgtgcacc atgtgcatgt cttcttatta aataaaatgt taaattcgta
      361 attaacgtaa acagaaaaat gtttaaaata taggcaatag tatacataac aattaattta
      421 cctatatttt tagctaagac ctgcatccct aatcgtactt gcaaaaattg aatatgatct
      481 aagttatttc tcttttgaag atacgtggca aactggtcaa ttttattatc aaaataattc
      541 aattttacac cactctcctc actgtcatta tacgatttag tacaatcttt tatcattata
      601 ttgcctaact gtaggaaata aatacttaac tgttaaatgt aatttgtatt taatatttta
      661 acataaaaaa aattacagtt aagaataaaa aacgactagt taagaaaaat tggaaaataa
      721 atgcttttag catgttttaa tataactaga tcacagagat gtgatggaaa atagttgatg
      781 agttgtttaa ttttaagaat ttttatctta attaaggaag gagtgatttc aatggcacaa
      841 gatatcattt caacaatcgg tgacttagta aaatggatta tcgacacagt gaacaaattc
      901 actaaaaaat aagatgaata attaattact ttcattgtaa atttgttatc ttcgtatagt
      961 actaaaagta tgagttatta agccatccca acttaataac catgtaaaat tagcaagtga
     1021 gtaacatttg ctagtagagt tagtttcctt ggactcagtg ctatgtattt ttcttaatta
     1081 tcattacaga taattatttc tagcatgtaa gctatcgtaa acaacatcga tttatcatta
     1141 tttgataaat aaaatttttt tcataattaa taacatcccc aaaaatagat tgaaaaaata
     1201 actgtaaaac attcccttaa taataagtat ggtcgtgagc ccctcccaag ctcgcggcct
     1261 tttttgtaat gaagaaggga tgagttaatc a
//
LOCUS       DAMQYT010000332         1209 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.340, whole genome shotgun sequence.
ACCESSION   DAMQYT010000332 DAMQYT010000000
VERSION     DAMQYT010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.340"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            113..1123
                     /gene="sspA"
                     /locus_tag="PGA33_002762"
     CDS             113..1123
                     /gene="sspA"
                     /locus_tag="PGA33_002762"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HDG6474495.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQS
                     SKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPD
                     AA"
BASE COUNT          454 a          221 c          192 g          342 t
ORIGIN      
        1 ttgtaaactc taatctttac aaaaactttt taatttattt atatattctt attgacttaa
       61 aaatttttag tgttatattt aacttgtaaa taaatttttt ggaggttttt agatgaaagg
      121 taaattttta aaagttagtt ctttattcgt tgcaactttg acaacagcga cacttgtgag
      181 ttctccagca gcaaacgcgt tatcttcaaa ggctatggac aatcatccac aacaaacgca
      241 gtcaagcaaa cagcaaacac ctaagattca aaaaggcggt aaccttaaac cattagaaca
      301 acgtgaacac gcaaatgtta tattaccaaa taacgatcgt caccaaatca cagatacaac
      361 gaatggtcat tatgcacccg taacttatat tcaagttgaa gcacctactg gtacatttat
      421 tgcttccggt gtagttgtag gtaaagatac tcttttaaca aataaacacg tcgtagatgc
      481 tacgcacggt gatcctcatg ctttaaaagc attcccttct gcaattaacc aagacaatta
      541 tccaaatggt ggtttcactg ctgaacaaat cactaaatat tcaggcgaag gtgatttagc
      601 aatagttaaa ttctccccta atgagcaaaa caaacatatt ggtgaagtag ttaaaccagc
      661 aacaatgagt aataatgctg aaacacaagt taaccaaaat attactgtaa caggatatcc
      721 tggtgataaa cctgtagcaa caatgtggga aagtaaagga aaaatcactt acctcaaagg
      781 cgaagctatg caatatgatt taagtacaac tggtggtaat tcaggttcac ctgtatttaa
      841 tgaaaaaaat gaagtgatcg gaattcattg gggcggtgta ccaaatgaat ttaatggtgc
      901 ggtatttatt aatgaaaatg tacgcaactt cttaaaacaa aatattgaag atatccattt
      961 tgccaacgat gaccaaccta ataacccaga taatcctgat aaccctaaca atcctgataa
     1021 ccctaacaac ccagatgaac caaataaccc tgacaaccct aacaaccctg ataatccaga
     1081 caatggcgat aacaataatt cagacaatcc agatgcagct taattaacaa ttaagttcgt
     1141 ctagataaaa tagaagcaaa gtcttagcaa cgtaaaatat tttgatttaa atggagggta
     1201 ttatatgaa
//
LOCUS       DAMQYT010000333         1200 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.341, whole genome shotgun sequence.
ACCESSION   DAMQYT010000333 DAMQYT010000000
VERSION     DAMQYT010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.341"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..200)
                     /locus_tag="PGA33_002763"
     CDS             complement(<1..200)
                     /locus_tag="PGA33_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474496.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEIS"
     gene            complement(262..546)
                     /locus_tag="PGA33_002764"
     CDS             complement(262..546)
                     /locus_tag="PGA33_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474497.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNKGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            complement(634..1146)
                     /locus_tag="PGA33_002765"
     CDS             complement(634..1146)
                     /locus_tag="PGA33_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474498.1"
                     /translation="MNSPKTIADNKYSEIQDDKFQLQPGDIIVTKGPVMWGFFGHCSI
                     AIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKVYRCSYPGAGKKAAEWVKKN
                     YENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSVKSYGLHIISPYAIKDNFID
                     PYKLRLVKAY"
BASE COUNT          380 a          184 c          179 g          457 t
ORIGIN      
        1 atacttattt ctaatgcttt ggggtctact gaaacaagta aagaatgatc gatgttacta
       61 atattgccat tctccaaatt tatttctgtg agtatttgga agctaccatt aggcaacggt
      121 ttaacaatag acaattgctt ttccgcttgt tgtattaaaa aaggttttgt agattgatta
      181 ttaatatgcc attcactcat gtatgttttt cactcctgct ttaaaatagg gttagaaagt
      241 ttatagttga gacattcatg ttcaaccaaa attttgttcg aattcaataa atgtcttgtt
      301 taaaatagaa atattgtaaa tgttatcgtc caaaacttca ccagttaagt atttgttttg
      361 aattaaaatt tggcagttag ttaagaagtc ttgataatca cgatcgcaaa aatagttttc
      421 acgtgcatct ttagcatcgc caaaaaagtt agcgactgtt tctgtttctc ctttattcga
      481 acgttcaata tataatttgt aaaatttagc tattgtatac ttttgttctt tagttagttc
      541 attcaaaata ttgggcctcc tgaaatatca tttgtaatct atacccaatt tattgcaaaa
      601 cacaaactaa tttaactatt tgatgaaact gtgttaataa gctttaacaa gccttagttt
      661 gtatggatct ataaaattat ctttaattgc atagggtgaa ataatatgta gtccataact
      721 tttaactgat ttttcactta caccaaattt ataagcttgg tagataattt tagtacaata
      781 cgtaaatttt ttgctgttca aatttaatgt aactagataa cgatgatttg tattctcata
      841 gtttttctta acccattcag ccgctttttt acctgcacca ggatagctgc aacgataaac
      901 tttcatccaa tcatttttgc cacttgcata attatattta aaagattcga aggattgtgt
      961 agttggtttg tcgccaggcc cctcaatttg taaaatcgtt ttatcatcaa tcgcgatact
     1021 acaatgacca aaaaatcccc acatgacagg gccttttgta acaataatat caccaggttg
     1081 taattggaat ttgtcatctt gaatttctga atacttatta tctgcaattg tttttggtga
     1141 gtttattggg gatacgacaa cgaataatat aagtaaaatt atcgttcgtt taatatagtt
//
LOCUS       DAMQYT010000334         1198 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.342, whole genome shotgun sequence.
ACCESSION   DAMQYT010000334 DAMQYT010000000
VERSION     DAMQYT010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.342"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..79
                     /locus_tag="PGA33_002766"
     CDS             <1..79
                     /locus_tag="PGA33_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDG6474499.1"
                     /translation="LFFFINMSSIIDKKIHVIYLRSYKY"
     gene            167..784
                     /locus_tag="PGA33_002767"
     CDS             167..784
                     /locus_tag="PGA33_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDG6474500.1"
                     /translation="MLCETEIINKNPKYRVIKYDDEYLMVDVIRTWLVYFFPFINWFI
                     PKRCAKISREEYEKLNTVKPVKNKNFWPVVGGTILLGATSRKYIHLLNIQLEKRSVIF
                     ICFVVFLCILIFFVLLNRKLKLKVFDNKKEEQKIILVPTLKNAVLILYGYLLIGGMSI
                     LALSMLLTLENQNLITFIAWGMGLMLFFLMNITLIVNKTVKVIKR"
     gene            859..>1198
                     /locus_tag="PGA33_002768"
     CDS             859..>1198
                     /locus_tag="PGA33_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDG6474501.1"
                     /translation="MLCESRVINQNPKYRIIKYNNEYFMVDLVSTWIAYFLPMINWFI
                     PKKYAKISREEFESLNIVKPAKNNTFWPVAGFAVLLTTLTRKYIYLLNIHLEKEIVIL
                     TCCMILLGVFA"
BASE COUNT          445 a          137 c          193 g          423 t
ORIGIN      
        1 gctatttttc tttataaata tgtcttcaat aatagacaaa aagattcatg ttatatattt
       61 aaggtcatat aaatattaat tttgatacaa aagggcacaa gtgttttgag aaacagaaac
      121 gataatatag ctgtgtgaat ttgttattaa taggaagtga taattattgc tttgtgaaac
      181 cgaaattatc aataaaaatc cgaaatacag agttattaag tatgatgatg agtatttaat
      241 ggtcgatgta ataagaactt ggcttgtata cttttttcca tttattaatt ggttcattcc
      301 aaaaaggtgc gccaaaatta gtagagaaga gtatgaaaaa ctgaacactg ttaagccagt
      361 taaaaataaa aatttttggc ctgttgtagg aggaacaatc ctactaggtg ctacttccag
      421 aaagtacata cacttactta atattcaatt agaaaaaaga tcggttatat ttatatgctt
      481 cgttgtattt ctatgtattt taatcttttt tgtgcttctg aatcgaaaat taaaattgaa
      541 agttttcgat aataaaaaag aagaacaaaa aataatttta gtaccaacat taaaaaatgc
      601 tgttcttata ttatatggct atttgttgat tggaggcatg tcaatattag ctttaagcat
      661 gttgctgaca ttagaaaacc agaatttaat aacttttata gcttggggta tggggttaat
      721 gttgtttttc ttgatgaata taacactcat tgtcaataaa acagtcaaag ttataaaaag
      781 gtgaataaat aactaattat attgtattgg ccgttcttca aataaaaaat aatttgaata
      841 aggtggaagt gataaacagt gctttgcgaa tctagagtca ttaatcaaaa ccctaaatat
      901 agaattatta aatacaataa tgaatatttc atggttgatt tagtaagtac ttggattgct
      961 tattttttgc ccatgattaa ttggtttatt cccaaaaagt acgcgaaaat tagtagagaa
     1021 gaatttgaaa gtttaaatat tgtcaaaccc gctaaaaata atactttctg gcctgttgca
     1081 ggatttgcag tgttattaac aaccttaaca agaaaatata tctatttgct taacatccat
     1141 ttagaaaaag aaatagttat attaacatgc tgtatgatac ttctaggtgt tttcgcat
//
LOCUS       DAMQYT010000335         1186 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.343, whole genome shotgun sequence.
ACCESSION   DAMQYT010000335 DAMQYT010000000
VERSION     DAMQYT010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.343"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            63..464
                     /locus_tag="PGA33_002769"
     CDS             63..464
                     /locus_tag="PGA33_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HDG6474502.1"
                     /translation="MKKNITKTIIASTVIAAGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERIDKLMKESDKNLTPYKAETVNDLYLIVKKLSQGDVKKAVVRIKDGGPRD
                     YYTFDLTRPLEENRKNIKVVKNGEIDSITWY"
     gene            424..516
                     /locus_tag="PGA33_002770"
     CDS             424..516
                     /locus_tag="PGA33_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474503.1"
                     /translation="MLKTVKSTRLLGIKKHTELNSCTPNVRKQC"
     gene            complement(833..1162)
                     /gene="ecb"
                     /locus_tag="PGA33_002771"
     CDS             complement(833..1162)
                     /gene="ecb"
                     /locus_tag="PGA33_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Ecb"
                     /protein_id="HDG6474504.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQ
                     TNFKKQVNKKVVDAQKAVNFFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLV
                     LKYNTLK"
BASE COUNT          413 a          182 c          169 g          422 t
ORIGIN      
        1 ttgtaaaata gcagtgtact cgcattagta agtacaaatt tattttacag ggagaactta
       61 ttatgaaaaa aaatatcaca aaaactatta tcgcatctac agtaatcgct gcaggtcttt
      121 taactcaaac taatgatgct aaagctttct ttagttatga atggaaaggt ttagaaatcg
      181 caaaaaattt agcagatcaa gctaaaaagg acgatgaacg tattgataag ttaatgaaag
      241 agtctgataa aaatctaact ccttacaaag ctgaaactgt taatgatctg taccttattg
      301 ttaaaaaatt aagccaaggt gatgtgaaga aagcagttgt cagaattaaa gatggtggtc
      361 ctagagatta ctatactttt gacttaactc gtcctttaga agagaacaga aaaaatatta
      421 aagttgttaa aaacggtgaa atcgactcga ttacttggta ttaaaaaaca tactgaatta
      481 aatagttgta cgccaaacgt tagaaaacaa tgctaacgta ttggcgtgct tttttatttt
      541 aagtaacttc caatttattt agcatcttta cgactgttta ataaagcacg tatgattaac
      601 acggttgcca ctatatccgt tacaattttt atgattgtta atacattcga tttccttttc
      661 acaatagcca cctcgcttgt tcaaaacata aacaactatt gcattcactt ttaagtaatt
      721 atttatatta tttatcccaa taagctcacc attcaaataa acacaatatt ataaaataat
      781 tatacctttg gatatagcaa aaagccacac tctatagcat ggcttctatc aattatttta
      841 aagtattata ttttaaaact agatcgattt gtctttgtaa ttttttcttt tcataactgt
      901 gttggaaatg aattaaatta acagctcttt gtgctttacg gtgtgttgca acagttcttg
      961 tacgtttgaa aaagtttaca gctttttgtg catccacaac ttttttattt acttgttttt
     1021 taaagtttgt ttgatactga tcatattttt tagcagcttc aacgttttta gtttgtgcat
     1081 gagattcacc ggcaaacgtt gataccgtta aactaatagc agctatgctt aaaattgatt
     1141 tcccaataaa attctttttc ataattaata acacctatcc tcaaat
//
LOCUS       DAMQYT010000336         1184 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.344, whole genome shotgun sequence.
ACCESSION   DAMQYT010000336 DAMQYT010000000
VERSION     DAMQYT010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.344"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            22..894
                     /gene="pmtC"
                     /locus_tag="PGA33_002772"
     CDS             22..894
                     /gene="pmtC"
                     /locus_tag="PGA33_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HDG6474505.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            894..>1184
                     /gene="pmtD"
                     /locus_tag="PGA33_002773"
     CDS             894..>1184
                     /gene="pmtD"
                     /locus_tag="PGA33_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HDG6474506.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYI"
BASE COUNT          451 a          130 c          214 g          389 t
ORIGIN      
        1 aaaatttaaa ggaggcagtc gatgaaatta gaacatatta caaaaaaata cggctcaaat
       61 gtcgttttaa atgatattga ttttgacttt ggcgatagta gaattgtcgg attaatagga
      121 aaaaacggtg ttggtaaaac aaccgttatg aaagtaatga atggtaatat tattaaattt
      181 gatggaaaag tagatattga taatgcagat aatatcggtt ttttaattga gcatcctaaa
      241 ttatatgata ataaatcagg attgtataac ttgaaattat ttgcacaagt attaggtaag
      301 ggttttgata aagcatacac agacaaaatt atagatgcat ttggtatgag accttatatt
      361 aaaaagaaag ttaagaaata ttcaatgggg atgaagcaaa agttagcaat tgcagtatct
      421 ttaatgaata aacctaaatt tttaatcttg gatgagccta caaatggtat ggatccagat
      481 ggctcaattg atgtgctgac tacaattaag tctttagtaa atgaacttga tatgagaatt
      541 ctaatatcaa gtcataagtt agaagatatt gaattaattt gtgatagagc tgtattttta
      601 agagacggac attttgttca agatgtaaac atggaggaag gtgttgcatc tgacacaacg
      661 atagttactg ttgatcataa agactttgat agaactgaaa aatatcttgc agagcatttc
      721 caattacaaa atgtcgacaa agcagacgga catttaatga tcaatgcaca aaaaaattat
      781 caagttatac taaaagcatt atctgaatta gatatttatc cgaaatatat tgaaacacgt
      841 aaaagttcat tgcgtgatac gtacttcaat ataaatcaaa gaggtgataa ataatgagaa
      901 ttttaaattt agttaagtat gatttttata gtatatttaa aagtccttta acatatttag
      961 cgatactagt cgtatctagt ttgattgcaa ctcaaagtat acttatggca aattcgatgg
     1021 ataacccgaa acatattatt gtctatggat ctgtatttgc tgcagcaaaa tggttattgt
     1081 taataattgg attaatgttt gttgttaaga caattacgcg tgatttttca caaggtacaa
     1141 ttcaactata tatgagtaaa gttaaaacac gcgttggata catt
//
LOCUS       DAMQYT010000337         1178 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.345, whole genome shotgun sequence.
ACCESSION   DAMQYT010000337 DAMQYT010000000
VERSION     DAMQYT010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.345"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            75..929
                     /locus_tag="PGA33_002774"
     CDS             75..929
                     /locus_tag="PGA33_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDG6474507.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
BASE COUNT          449 a          158 c          209 g          362 t
ORIGIN      
        1 ggttttaata atgttaaact ttattatcgt agtcaaactg aatgtataac aacaatgacc
       61 taagaggtgt ggatatgaat aaacacaaga aaggttctat ttttggaata ataggacttg
      121 ttgtcatatt tgctgttgtc tcatttttat ttttctcaat gatatccgat cagatatttt
      181 tcaaacatgt taaatccgac attaagattg aaaagttaaa tgttacatta aacgatgcag
      241 caaagaaaca aataaataat tatacgagtc aacaggtatc aaataaaaag aatgatgcat
      301 ggagagatgc atctgcaact gaaattaaaa gtgcaatgga tagcggtact tttatcgata
      361 atgaaaagca aaaatatcaa tttttagatt tatcaaagta tcaagggatt gataaaaata
      421 gaattaaacg tatgttagta gatagaccaa cgttattgaa acatacggat gatttcttaa
      481 aagctgctaa agataagcac gttaacgaag tttatttaat ttcacatgca ttattagaaa
      541 ctggcgcagt taaaagtgaa ttagctaatg gagtcgaaat tgatggcaaa aagtactaca
      601 atttctatgg agtaggagcc cttgataaag acccaattaa aacaggtgca gaatatgcta
      661 aaaagcatgg ttgggataca cctgaaaaag ctatttcagg cggtgctgat ttcattcata
      721 agcacttctt atcaagcaca gatcaaaata cattgtatag tatgagatgg aatccaaaaa
      781 atccaggaga acatcaatat gctacagata ttaagtgggc agaaagtaat gcaacaatta
      841 tcgctgactt ttataagaac atgaagactg aaggaaaata cttcaaatac tttgtgtata
      901 aagatgacag taaacatttg aataagtaat ttgataagct acgagttgtt tttatgactc
      961 ggacatacta aaaagacgct ttctatcttg ttttgataga aagcgtcttt ttgcattaga
     1021 gaaaacacat tgattgataa tcccaccaat gcaagtgggg caggacatcg ataaagaatt
     1081 actttttctt tagaaattag tatttcttat gcatgagttt tactcatgta ttcctatttt
     1141 taagtacaca ttagttatag ctaatgataa agaaccac
//
LOCUS       DAMQYT010000338         1178 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.346, whole genome shotgun sequence.
ACCESSION   DAMQYT010000338 DAMQYT010000000
VERSION     DAMQYT010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.346"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            33..1160
                     /locus_tag="PGA33_002775"
     CDS             33..1160
                     /locus_tag="PGA33_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDG6474508.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSNIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
BASE COUNT          423 a          183 c          247 g          325 t
ORIGIN      
        1 tcaactcaat aattttaagg aggaattaag taatgaaaag taaatacgaa ccattgtttg
       61 ataaagtaga attaccaaat ggagtagagt tgagaaatcg atttgtgtta gcccctttaa
      121 cacatatttc ttcaaatgat gatggtacta tttcagatgt agaacttcct tatattgaaa
      181 agcgttcaca agatgttggt attacaatta atgctgcgag taatgtgagt gatgtcggaa
      241 aagcatttcc aggacagcca tcaatcgcgc atgacagtaa tattgaagga ctaaaacgat
      301 tagctacagc aatgaagaaa aacggtgcca aagcactcgt acaaatacat catggcggtg
      361 cacaagcatt gcctgaatta acacctgatg gagacgtcgt agcaccaagt ccaatttctt
      421 taaaaagttt tggtcagaaa caagaacata gtgctagaga aatgacgaat gaagagattg
      481 aacaagcaat caaggatttt ggtgaagcaa cgcgacgtgc aattgaagca gggtttgatg
      541 gtgttgaaat acatggcgcg aatcattact taattcatca atttgtatca ccatactata
      601 atagaagaaa tgatgtatgg gcaaatcaat ataaattccc ggtcgctgtg attgaagaag
      661 tacttaaagc gaaagaagcg tatggcaata aagactttat agttggatac agattatctc
      721 cagaggaagc ggagtctcca ggaatcacaa tggaaattac agaggaactc gttaataaaa
      781 ttagccatat gccaatcgac tatattcatg tttcaatgat ggatacgcat gcaacgacac
      841 gtgaaggtaa atacgctgga caagaaagac tgcctttaat tcacaaatgg ataaatggtc
      901 gtatgccact tatcggtatt ggttcaattt tcacagctga cgaagcttta gatgcagttg
      961 aaaatgttgg tgttgactta gtagccattg gtagagagct actactggat tatcaatttg
     1021 ttgaaaaaat taaagatgga cgggaagatg aaattattaa ttactttgat ccagagagag
     1081 aagataatca tcacttaact cctaatttat ggcatcaatt taatgaaggg ttttatccat
     1141 taccacgtaa agataaataa gtgtttaact gttaccac
//
LOCUS       DAMQYT010000339         1174 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.347, whole genome shotgun sequence.
ACCESSION   DAMQYT010000339 DAMQYT010000000
VERSION     DAMQYT010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.347"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            105..1052
                     /locus_tag="PGA33_002776"
     CDS             105..1052
                     /locus_tag="PGA33_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDG6474509.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
BASE COUNT          441 a          147 c          245 g          341 t
ORIGIN      
        1 aaatagttat acgtctacag aagtatagta aaaaaattat tttactatat aataaataga
       61 gaaaacagac aaagtatgta aggaatagta aaggagtggt gggattgaaa cgttcgtcta
      121 aaagtaaaat gagcctaccg aaaaaaatat ttttatgggt attcggtatt ttagtaatct
      181 tagcgattgt agctgttgtg tatgtagctg ctaaaatttt tattactggt aataagatac
      241 acaatccgtt agatcgcaat cattcagaat taagagataa aaaagtgagt ttaaacgatg
      301 gtgatccatt cacaatcgct ttatttggtg tcgattcaga tgctgatcgt aagaaaaagg
      361 gtggcggaga gcgtagtgat agtattatga ttttatctat caaccctaaa acgaagaaaa
      421 ctgaaatagt aagtataccg cgtgatacaa gagcggaaat tgttggccgt ggtactactg
      481 aaaaaattgc acatgcctat gcatatggtg gaccaaatat ggctgttaaa tcacttgaaa
      541 aattaatgaa tgtgccaatt gatcattatg cgacaatcga tatggatggt ttgcataata
      601 tgatagatag catcggcggt gttgatgtag taagtaatga tacattcact gttgatggtg
      661 ttcgttttac aaaaggacag cagacacatg taaatggtga tcaagcattg aagtttatta
      721 gaagtcgtaa agaagaaggt gctggtggtg actttggtcg acaacaacgt cagcaaattg
      781 tgcttgaagc gatggccaat aagattgcaa gcccatcatc gattacacat tttaatagtc
      841 taatgaatga aattcaaaat aatgttaaaa cagatttaac attaggtgat ttgaatacga
      901 ttagaagcaa ttataaagat gctaatgaca cgattaataa acatcaattg agtggtcaag
      961 gcggcattca aagtgacgga ttgtactact tcattcctag tgagcagtca aaagctgaaa
     1021 gtactaagtt attaaaagat aatctagagt agtaaactaa gggaaaataa gttatcaaaa
     1081 ttgtacacaa attaattaga tagaatgatc tgtaaataga ataacgaata gaactaatag
     1141 cccgtaaata gaaggtgata acactttgta ttta
//
LOCUS       DAMQYT010000340         1171 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.348, whole genome shotgun sequence.
ACCESSION   DAMQYT010000340 DAMQYT010000000
VERSION     DAMQYT010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.348"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..189
                     /locus_tag="PGA33_002777"
     CDS             <1..189
                     /locus_tag="PGA33_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDG6474510.1"
                     /translation="ALSSFIYIEKIIEYILVPNVVESKFIIIVSMLVIAGAQFVAFQF
                     IMQYSRFYIFELNTEDDD"
     gene            336..947
                     /locus_tag="PGA33_002778"
     CDS             336..947
                     /locus_tag="PGA33_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HDG6474511.1"
                     /translation="MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAIVFVIIGI
                     TYIFIGDSYDQLTNFNVILYMGSLFYAWMAIRNLWNRPLLLKYKNITDSSSGIVNKSE
                     YNSVESLRINIEIAKYKGIISLIVAIVLTVLMTLKSTPQITAETRDLSISFFILSLFI
                     IIIFAVWDLFIRVRKGAFAFVVIRPILFSCWLFILNMILSRLL"
BASE COUNT          411 a          119 c          197 g          444 t
ORIGIN      
        1 gcgttgagct catttatata tattgaaaaa attatcgaat atatattggt tcctaatgtc
       61 gtagaatcta agtttataat tattgtgagc atgttggtta tcgctggagc acaatttgtg
      121 gcatttcaat ttattatgca atacagtaga ttctatattt ttgaattaaa cactgaagat
      181 gatgactaaa caattttata aattctgtga agtatactta tatataagat aatcaacttc
      241 aacgtatatc accatattat tttatactca atgatgtgaa tggatttaag tggattgata
      301 ttaaagtaaa agttatggaa tattgaggtg tgaatatgga gttcttatta ttaattgtcg
      361 tagccggact gtattatatt atatatttaa ctgctgtgat gtattctgaa aaaatagtag
      421 tattgcctat aatcatctat gccattgtgt ttgtaataat tggtatcact tatatcttta
      481 taggcgacag ctatgatcaa ttaacaaatt tcaatgtgat tttgtatatg gggagtttgt
      541 tttatgcatg gatggctatt agaaatcttt ggaacagacc actattacta aaatataaga
      601 acattacaga tagttcaagt ggaatagtta ataaatctga atacaattca gttgaaagct
      661 tacgtataaa tattgaaata gctaagtata aagggattat ttctttgata gtagctatag
      721 tactaacggt attaatgaca ttaaagtcaa cacctcaaat cactgcagaa acacgtgact
      781 taagtatctc atttttcata ctcagcttat ttatcattat tatatttgca gtttgggatt
      841 tgtttattag agttagaaaa ggagcgtttg cttttgttgt aataaggcca atattattta
      901 gttgttggtt atttattttg aatatgattt tatcaagatt attataattg tattacatat
      961 aggtgtgggt aaatttttat ttatgaaatg ataaaaaagt atatactatt aatatctttt
     1021 ttactataat tataaatagt taaataaatt gaaagacaaa ataatgagta ggagttaatg
     1081 agatggaaaa ccaaaaacaa ggcaatggct taaaaattgc aacatgggta tttattgtat
     1141 taacagtagt tacaccgcta tttggtattg g
//
LOCUS       DAMQYT010000341         1169 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.349, whole genome shotgun sequence.
ACCESSION   DAMQYT010000341 DAMQYT010000000
VERSION     DAMQYT010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.349"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            1..833
                     /locus_tag="PGA33_002779"
                     /pseudo
     CDS             1..833
                     /locus_tag="PGA33_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            979..>1169
                     /gene="sarX"
                     /locus_tag="PGA33_002780"
     CDS             979..>1169
                     /gene="sarX"
                     /locus_tag="PGA33_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HDG6474512.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELD"
BASE COUNT          467 a          129 c          180 g          393 t
ORIGIN      
        1 ttgaacatta cctgtttatt tgaaaaacct tcagttttta aatcactagt atcacaaata
       61 aagcgactta aattcgattc gttaataata gataatgcaa atttaagtag cccttatttg
      121 atgggggaaa gtgatgagtt actattgaaa aatattttgc attttaaaaa tttaaaacaa
      181 gtaattaatg aattggatat tgaacaagaa aagctgattt ttctaaatgt tgaaaatcat
      241 aaactgctta ataataaaga acgagatcta agtaatagtg ctccattaat ttataagaca
      301 ttaagtgcgc tgtatcacaa ctttgatggc tttggattaa acatttttga caatcatcaa
      361 gcatttaatg cgatgcatct atatgacaaa aatggattta aaacaacact aggtcttata
      421 ttagaaaagt ttatcgaata tgtctcgaaa ccaaaatacg aaaacagtta ttattctatt
      481 tttgatatag agaattatta ttgtcttgtt atttatgatt ggcgagtgat agagagcgag
      541 acaattatga gtaattttga ggatagtcaa gtttatataa attttaaaaa caatgtttta
      601 aacgataaat atctaattgt aatagaaaca ttggacaaaa tagtggcaac attaatcatt
      661 tgatttctaa agaattaaga gataaatatg aatggaaccc tagtttacta tctaaaattg
      721 acaactacct taaaccagca atagagatta aagagcataa ttttagtgat aattccttga
      781 atattaacgt tacttttaat gcgttataca taattaaaat aggaaaaaaa taacaccttg
      841 atatgtattg caaaatttaa tttgcatttg ttggtaattt tgacaacgta ttaaaaacaa
      901 atgagaaaga caatggctga aaaatttaag ataataatga cagaagcatt gtctttatat
      961 atttgggggt gcaacatttt gaatactgag aaattagaaa cattgcttgg cttctataaa
     1021 caatataaag cattatctga atatattgat aaaaaatata agttgtcgct aaatgattta
     1081 gcagtcttag atttaacgat gaagcattgc aaagatgaaa aagtacttat gcaatcattt
     1141 ttaaaaactg caatggatga gctagattt
//
LOCUS       DAMQYT010000342         1163 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.350, whole genome shotgun sequence.
ACCESSION   DAMQYT010000342 DAMQYT010000000
VERSION     DAMQYT010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.350"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..595)
                     /locus_tag="PGA33_002781"
     CDS             complement(<1..595)
                     /locus_tag="PGA33_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474513.1"
                     /translation="MKHKSVYLLCFLILPILLLTSVSAYAVSNPVGEPSHAVKPKIEK
                     YDLSHYRSIYSEKGDWNPFGQEEISRQINNVSDFFFSMTKILAGVTDYAIENLFQLDV
                     INDFADKIGDFVGDIYQKLLSNLALTLFIIVCFNAFIIFSVQGNAREALKRAFLIMCL
                     IGFGVGILANAGNIIRGTNNIGKDLNNIIMNSTSTIDG"
     gene            complement(600..>1163)
                     /locus_tag="PGA33_002782"
     CDS             complement(600..>1163)
                     /locus_tag="PGA33_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDG6474514.1"
                     /translation="FMKRVIGDDKVADTKGQILKQLREANNEMEERFRLMNDSSDYKI
                     GNDFRNFDMRPYFIIFDEVTAFTSTLDKKELQEMNDYLINIIMKGRQAGVFMFLTAQR
                     PDADVIKGNVRDQLGLRVSLGNLSNDGYRMTFGQTDKEFQTIHDSDIGRGYISILGQY
                     NEPILFDAPLMEQYDFVEDVKQILNKE"
BASE COUNT          425 a          227 c          125 g          386 t
ORIGIN      
        1 tcccatcaat agtagatgta ctattcataa ttatattatt caaatcctta ccaatattgt
       61 ttgtaccacg tataatatta cctgcattcg caagtatacc aacaccaaaa ccaattaaac
      121 acataattaa aaacgctcgt tttaatgctt ctcttgcatt accttgtaca ctaaaaataa
      181 taaatgcgtt aaaacaaaca ataataaata atgttaatgc taaattactc aataactttt
      241 gatatatatc gccaacaaaa tcgccgattt tatccgcaaa atcattaata acatctaatt
      301 gaaataaatt ttcaatcgca taatctgtaa cacctgctaa aatcttagtc atagaaaaga
      361 aaaaatctga cacattatta atttgacgtg aaatttcttc ttgaccaaac ggattccagt
      421 cgcctttttc actatatatt gaacgataat gtgataaatc atacttttca atttttggct
      481 taactgcatg tgagggctct ccaactggat tagaaacagc ataagcactc acagatgtta
      541 aaagcagaat aggcaaaatt aaaaagcata aaagatacac acttttatgc ttcatcaaat
      601 cactccttat ttaatatttg tttcacatct tccacaaaat catattgttc cattaaaggt
      661 gcatcaaata aaattggttc attatattgt ccaagtatag aaatataacc tcgaccgatg
      721 tcactatcat gaatcgtttg aaattcttta tcagtttgac caaaggtcat acgataacca
      781 tcatttgaca aattacctaa agacacacgt aaccctaatt ggtcacgcac attaccttta
      841 atcacatctg catctggtct ttgtgctgtt aaaaacataa atacaccagc ttgacgacct
      901 ttcataataa tattaattaa ataatcattc atttcttgta attctttttt atctaaagtt
      961 gaagtaaaag ccgttacctc atcaaaaata atgaaatagg ggcgcatatc aaaattacgg
     1021 aaatcattcc cgattttata atccgaacta tcattcatta atctaaaacg ctcttccatt
     1081 tcattattag cttctctcaa ttgttttaat atttgacctt tagtatctgc aactttatca
     1141 tcaccaataa cgcgcttcat aaa
//
LOCUS       DAMQYT010000343         1145 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.351, whole genome shotgun sequence.
ACCESSION   DAMQYT010000343 DAMQYT010000000
VERSION     DAMQYT010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.351"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            40..531
                     /locus_tag="PGA33_002783"
     CDS             40..531
                     /locus_tag="PGA33_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDG6474515.1"
                     /translation="MTFEEKLSEMYSEIANKIISMIPVEWEKVYAMAYIDEECGEVFY
                     NYTEPSSDELFYYTSVIKKYNLLKSSFMDSVYELHDQFEELREVFIEEGLEPWTSCEF
                     DFTKEDKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPETEYEMEEVKEIEQYIKE
                     QEE"
     gene            741..>1145
                     /locus_tag="PGA33_002784"
     CDS             741..>1145
                     /locus_tag="PGA33_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HDG6474516.1"
                     /translation="MTTKENIDILRKPGAQALSLASLFMILFSCLTFFFGLDYERFPN
                     YLKITTIIELIIIIISLLQWIRFIDFEKESAQKYKKIYARFLVIINVLTTITAVFATC
                     NLYYFVAVQNHYDLFNYWLMGTISIIISYLLLV"
BASE COUNT          444 a          119 c          199 g          383 t
ORIGIN      
        1 ttaaagagca agatgaagct gaactatagg ggcgataata tgactttcga agaaaaatta
       61 agtgaaatgt atagcgagat tgcgaataag attattagca tgataccggt agagtgggaa
      121 aaagtatatg caatggcata tatcgatgaa gaatgtggag aagtgtttta caattatact
      181 gaaccaagca gtgatgaatt attttactat acaagtgtga taaagaagta taatttattg
      241 aaatcaagct ttatggattc agtatatgag ttgcatgatc aatttgagga actaagagaa
      301 gtatttatag aagaaggtct tgaaccatgg acatcatgcg aatttgattt tacaaaagaa
      361 gataaattaa aagtttcatt tgattatatt gattggataa atacagagtt tgatcaatta
      421 ggccgtgaaa attattatat gtacaaaaaa tttggtgttt taccagaaac ggaatatgaa
      481 atggaggagg ttaaagaaat cgagcaatat attaaagagc aagaagaata gacaatctta
      541 atgtaagatt aaacaataaa gctttgttta gtctttttag cgtttaagta aaaaacaata
      601 gagaccataa agttgtcatc aaataataat ataaagataa ttttaggctt ttatactttt
      661 aatcgaaaag aatctaagta cttataactt taaatactta atatgctatt acaaaatcaa
      721 tgtataggag ataggtaaat atgactacta aggaaaatat agacattctt cgaaagccag
      781 gtgcacaagc cttaagttta gcatcattat ttatgatact tttttcatgt ctaactttct
      841 tttttggttt agattatgaa aggtttccaa attatttaaa gataacgaca attatagaat
      901 taataattat cataattagt ttacttcaat ggattagatt tatagatttc gaaaaggaaa
      961 gcgcacagaa atataagaaa atatatgccc gatttttagt tattataaat gtgctaacta
     1021 ctatcaccgc agtatttgca acatgtaacc tttattattt tgttgctgta caaaatcatt
     1081 atgacctatt caattattgg ttgatgggta cgatttcaat cataattagt tatttgttat
     1141 tagta
//
LOCUS       DAMQYT010000344         1141 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.352, whole genome shotgun sequence.
ACCESSION   DAMQYT010000344 DAMQYT010000000
VERSION     DAMQYT010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.352"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            119..1132
                     /locus_tag="PGA33_002785"
     CDS             119..1132
                     /locus_tag="PGA33_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDG6474517.1"
                     /translation="MAIKYSALNLVPIREGEDEQTAINDMVNLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
BASE COUNT          399 a          192 c          202 g          348 t
ORIGIN      
        1 tattttttta tttgtaaaat gtaacgtact attattttgg ttatgagcaa tttaatattt
       61 atctggttat tcgattggta tacttcttat atcataaaaa aggaaggacg atataaaaat
      121 ggcgattaaa tattcagcat taaaccttgt ccctattcga gaaggtgaag atgaacaaac
      181 agcaattaat gatatggtta atctcgcaca acatttagac gaattatcat atgaaagata
      241 ttggattgct gaacaccata acgctcccaa cctagtaagt tcagcaactg ctttattaat
      301 tcaacatacg ttagaacata cgaaacacat acgtgtaggt tctggaggca tcatgttacc
      361 taatcatgct ccattaatcg ttgcggaaca atttggcacg atggcaacat tatttccaaa
      421 tcgtgtcgat ttaggattag gacgtgcacc tggaacagat atgatgaccg caagtgcatt
      481 aagacgagat caacatgatg gtgtttataa atttccagaa gaggtttcat tattacaaca
      541 atatttcggc cctgctcacc aacaagcata tgttcgtgct tatccagcag taggtaaaaa
      601 tgtgccttta tacattcttg gttcttcaac agattctgca catttagctg ctcgcaaagg
      661 gcttccatat gtgttcgctg gacattttgc acctcaacaa atgaaagaag ctatcgaaat
      721 ttacaaaacg ttatttgaac cttctgatgt attagacgaa ccttatgtta ttgtatgttt
      781 aaatacaatc gttgctgaaa atgatgacga agcacaatat ttagcttcat ctatggcaca
      841 agtaatggtt agtatcactc gtggcagaat gcagcccgtt caaccgccaa cacatgaact
      901 acaaaatata ttaacgccga gagaatacgc gatggctatg gaaagacaga aaatatcatt
      961 aataggttca gaaaatactg ttcaacaaaa aattcaagat tttatggaaa cttatggtga
     1021 agtcaacgaa attatggcaa taagttatat ttatgataaa gatatgcaat tagactctta
     1081 tcgtcggttc aagaatgtta taaatcagat aaatgaaaaa aacactttat aatgtgataa
     1141 a
//
LOCUS       DAMQYT010000345         1130 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.353, whole genome shotgun sequence.
ACCESSION   DAMQYT010000345 DAMQYT010000000
VERSION     DAMQYT010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.353"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            208..>1130
                     /locus_tag="PGA33_002786"
     CDS             208..>1130
                     /locus_tag="PGA33_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474518.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLCFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQIIILSMIAALIIYLLLR
                     SDRYVHLTILKRRIIMIGFITLPLYYLLFYN"
BASE COUNT          372 a          151 c          174 g          433 t
ORIGIN      
        1 acttgaatag acaagtgtaa tcattcgtca tagaaaaaga cgaggggcga tttagataaa
       61 ttgttgaaag taatattaaa taccttgttt atctaatttg attgaggaaa catgcatagg
      121 tgaaaattag aaatacatta aaaatattga attaattttt cgacctacaa gttgaggaaa
      181 atttaactag atttgaggat tagcaaagtg aaaacaattc aattacacaa gacaactttg
      241 atattaattg cgatattatt gttttatact tttatgggta tcttactacc actcatgcac
      301 gatgatttac aatggtttag caattataat actgatattt taaaagtagg atttgcatca
      361 cttaatggtc gctatatcgg aaatatcttt gaaattatag ctgtacatgt aagctggtta
      421 cgttggcttt catatggcct cattagtatg ggcatcattt ggatgattat gcacattaca
      481 cgttgtaaag cgtggacaag ctattatttg ttagcatttt ctttaatgtt aattttacca
      541 agtgctattt atgcagatac atatggctgg tttgcaggat tttataatta tgcgacatca
      601 acactaattt cactctttat tatttattat tgtattaatg caattatata taaagaaaag
      661 cagccggtaa gtgttactgt actattctat gttttatgct tcttcggtca attatttatg
      721 gagaatgtga cattattcca ctgtatcatt ttaattttag cttttctgta tgaatttact
      781 gcaaatcgaa cacttaatta taaattattg ttttcattta tgattgctac tataggtaca
      841 atcattatgt tttccaatcc aaattatcgc aaaattttat ttgaaggatc agaatatcaa
      901 caagtttcaa ataatcaagg tatcttttca aaatttgcag aaatgatatc aacgtcactt
      961 ccatatggtg tcattttcag tcagattata attttaagta tgattgcagc gcttattatt
     1021 tatctactgc ttagaagtga tcggtatgta catttaacaa tattaaagcg tcgtatcatt
     1081 atgataggat ttatcacttt accgctttat tatttactat tttacaatca
//
LOCUS       DAMQYT010000346         1130 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.354, whole genome shotgun sequence.
ACCESSION   DAMQYT010000346 DAMQYT010000000
VERSION     DAMQYT010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.354"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(180..1082)
                     /locus_tag="PGA33_002787"
     CDS             complement(180..1082)
                     /locus_tag="PGA33_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDG6474519.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
BASE COUNT          348 a          200 c          150 g          432 t
ORIGIN      
        1 tattccttat tagaatattg tgaatctgct gtaaaataat caactacttc ttaatatcaa
       61 tatttcactt tcatctcaaa atggtaacat tataaataat ttatctttaa caccttttta
      121 gaaaagcaag aaaaaactaa ccaatctata taaagactgg ttagcttttt aaatgataat
      181 tatttagcga tataagttgt cagcgttcca atattatcaa tagtcacttt aacttcatca
      241 cctggttgta aaaatttagg tggttgcata cctgcaccaa cgcctgctgg tgtaccagtt
      301 gcaataatat ctcccggatg tagtgcaaca tattttgaaa tttcttctat taattcatca
      361 attttaagaa tcatttcgcc agtgttacca tcttgtctaa tttcattgtt aacttttgta
      421 acaatattta cattttcagg taatggtagt tcgtctttag taacgatata aggacccatt
      481 gggcaaccgc cagttaaact ttttgataaa aatgcttgat cttgttcact ttgtgctttg
      541 cgatcagtga tatcgttaat aattgtatag ccgtaaacat aatctaaagc taatgctttt
      601 ggaatctttt caccagactt accaataaca atacctaatt caccttcata atctaattga
      661 tcagtaatat ctttatgatt tggaattgtt gcattatctc ctgttaaaga tgacgctgct
      721 tttgtaaata catataattt ttccacttca tgatttaatt cgttcgcatg atctttgtaa
      781 tttctaccaa aagcaatcac attattcgga ggtgttactg gtggtaaaaa ttcaatgtca
      841 ttaaatgaaa ttttatagtc ttcagctttg ccgctatctt ctgctgctac aactgcttta
      901 cgtacttgtt cttgaaaatc taaagtatga ttttgttgta aaccagctaa caatgtttta
      961 ggatggaaat ctccttctgc aaagtcagca aatacttgtg ttaaatccca tacagcatct
     1021 tcgcgtttta ctttaacgcc atatgaagtt ttgtcattat acttgaatga taagaatttc
     1081 attcattctc aactcctcat ctttatctta attcacatta taactttttt
//
LOCUS       DAMQYT010000347         1125 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.355, whole genome shotgun sequence.
ACCESSION   DAMQYT010000347 DAMQYT010000000
VERSION     DAMQYT010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.355"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..150)
                     /locus_tag="PGA33_002788"
     CDS             complement(<1..150)
                     /locus_tag="PGA33_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDG6474520.1"
                     /translation="MKREVILKLQQFIIERENADKRRQYKKENEELDLSLTQFHIIEL
                     IDNNDK"
     gene            584..>1125
                     /locus_tag="PGA33_002789"
     CDS             584..>1125
                     /locus_tag="PGA33_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDG6474521.1"
                     /translation="MDVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNKPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSH"
BASE COUNT          394 a          175 c          152 g          404 t
ORIGIN      
        1 tttatcatta ttatcaatca actctatgat atgaaattgt gttagagata aatctagttc
       61 ctcattttcc tttttatact gtcttctttt atcggcattt tctctttcta taataaattg
      121 ttgtaatttt aaaataactt ctctcttcat atccactatt attcctcctc ttcattagtt
      181 aactggttaa ctatattatt gttgtcactc ccatttttgt caaatattgt tgtaattaac
      241 atatatttac ttagttatgc accatttatt ccgcatttca caaatccacc tataatacaa
      301 actatcattt taaataagca gaatttaccc tgtcttccac acaaattgtt ttcgacactt
      361 taatgtgcct actatccaaa aaagttactc ataacaaatt gcgcatttta ataaatgtag
      421 caatgccatt tgcatgctgc aaaaatactc gtattcatat ttatgtttta tattattaaa
      481 gttttttaaa tccatatttt tataacactt gctatgtgat aaaattaatt ttatatataa
      541 aatcttaaga ttcagattat ttaatagcaa aggagatagt gatatggatg ttttaacaat
      601 agaacattta acaaagaaga taggcaacaa aacgattctc gaagatgtat catttaagct
      661 gaaacgcgga caaatagttg gtctcgttgg agcgaatggt gcaggtaaaa caactttaat
      721 gaaagttata ttaggttact ctagtttcca aagcgggaat tttaatgtta ttaacagcaa
      781 ggacagcaaa agcaatatcg gcgcattgat tgaaaatcca ggaatatatc cttttatgtc
      841 tggatatgaa aacttgaagt tattgaatga atcaaaaaac actcaagata tcgataaaat
      901 tgtctcacaa cttcatatgg atgaatacat tcataaaaaa gctaaaacgt attctcttgg
      961 tatgaaacaa aaattaggaa ttgctatagc atttttaaat aaacctcaat tcattatctt
     1021 agatgaacca atgaatggct tagatccaaa agctgtgcga gatgtacgtg aattgattgt
     1081 ccaaaaagcg caagaaggtg ttactttctt aatttcgagt catat
//
LOCUS       DAMQYT010000348         1115 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.356, whole genome shotgun sequence.
ACCESSION   DAMQYT010000348 DAMQYT010000000
VERSION     DAMQYT010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.356"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            128..1003
                     /locus_tag="PGA33_002790"
     CDS             128..1003
                     /locus_tag="PGA33_002790"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDG6474522.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNDQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QISYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
BASE COUNT          355 a          164 c          186 g          410 t
ORIGIN      
        1 aagaaaatga atatatacat ctttagtcta gtattataac tagcatgtct ttaaatgaat
       61 atgctatcat gatatggtaa ttttgatatt taaataaatt aaattgagta atgaaagtgg
      121 gtacattatg tttatcattg aattaataaa aggtattatc ttaggggtcg tcgaaggatt
      181 aacagaattt gcacctgttt cctctactgg acatatgatc ctagttgatg atatgtggtt
      241 aaaatcatct gaatttttag gttctcagtc agcatttaca tttaaaatcg tcatccaatt
      301 aggatccgtc tttgcagcag catgggtgtt ccgcgaacgc ttcttagaga ttttacatat
      361 tggtaaacac aaacatgttg aaggagataa cgatcaacaa agacgttcaa agccaagacg
      421 tttaaattta ttacatgtat tagtgggtat ggtgccagca ggtattttag gcttactatt
      481 tgatgatttc atcgaagaac atttatttag tgtgccaact gttatgattg gcttattcgt
      541 aggtgctatc tatatgatta ttgctgataa atactcagct aaagttaaaa acccacaaac
      601 agtagatcaa atcagttatt tccaagcatt tgtaattggt atttctcaag cagtagctat
      661 gtggcctggt ttcagccgtt caggctcaac aatttcaact ggggtattaa tgaaattaaa
      721 tcataaagca gcatcggact ttacatttat tatggctgtt ccaattatgt tagcagctag
      781 tggtttatct ttattaaaac attatcaaga tattcagatt gctgatatac ctttttatat
      841 tttaggcttt ttagctgcat ttactgttgg actaattgca atcaaaacat tcttgcatct
      901 aattaacaaa atcaaactaa ttccatttgc catttacaga attgtattag taatctttat
      961 tgctatactt tacttcggat ttggcattgg taaaggtatt taatttcctt ttaaaatata
     1021 gactgtcaat taaggaatca cttgattggc agtttttatt gtcttctttt aaaaaacatc
     1081 tgaaatactt aatagtgatt cactactatc atcgt
//
LOCUS       DAMQYT010000349         1115 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.357, whole genome shotgun sequence.
ACCESSION   DAMQYT010000349 DAMQYT010000000
VERSION     DAMQYT010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.357"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            1..1115
                     /locus_tag="PGA33_002791"
                     /pseudo
     CDS             1..1115
                     /locus_tag="PGA33_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          459 a          136 c          208 g          312 t
ORIGIN      
        1 gtaatttgaa tggtgctgct gatattaaaa tatatgagtt attagatgat aaaagtaaac
       61 caactggtca acagacaata atttatcaag gaacatctaa tgaggcaatt aatccaaata
      121 atccattaaa atcatcgggg tttggagatg attggctcca aaatgctaaa ttaatgaata
      181 atgataatga aagcacagat tatttaaagc aaacagatca attatcaaat caatataaaa
      241 taaagttaga agatgcagat agattatcaa atagtgattt tttaaaaaaa tatagaatgg
      301 aatcaagtaa cttcaaaaac aaaaccattg tggcggatgg cggtaattcg gaaggcggtg
      361 caggagcaaa atatcaagga gcgaaacatc cgaatgaaaa agttgttgct actgactcag
      421 caatgattcc ttatgctgct tggcagaaat ttgctagacc acgctttgat aatatgatta
      481 gttttaatag taccaacgat ttattaacat ggttacaaga tccattcatc aaagatatgc
      541 caggaaaacg cgttaacatt aatgatggtg tgcccaggtt agatacttta atagacagcc
      601 atgtaggtta taaaaggaag ttaaatagaa aagataacac atacgatact gtaccactaa
      661 tcaaaataaa gtcggtaaaa gatacagaaa ttaaaaatgg aaaaaagtaa aaaagactat
      721 taacataaca ttagatatgg atgggcgaat tccaataaat gtttggacag gagattcgat
      781 tgcacgttct ggaagaggaa ctttaattaa acttaattta gaaaatcttg atgcgttgag
      841 taaactgatt actggtgaaa caagtggtat gttagcagaa tgcgtaatct ttttaaatga
      901 aagttttaac atctcagaaa atgaaaataa aaattttgca gatagaaaga aacaattatc
      961 agaaggattt aaggataaga ttaacttatt tcagttagaa gaaatggaaa gaactttaat
     1021 tagtaaaata aactcacttg aagaagttgc agatgaaaca atagaaagta ttagtgctgt
     1081 taaacactta ttacctgatt ttgcattgga tgcat
//
LOCUS       DAMQYT010000350         1110 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.358, whole genome shotgun sequence.
ACCESSION   DAMQYT010000350 DAMQYT010000000
VERSION     DAMQYT010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.358"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            100..663
                     /locus_tag="PGA33_002792"
     CDS             100..663
                     /locus_tag="PGA33_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDG6474523.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            complement(729..>1110)
                     /gene="pmtD"
                     /locus_tag="PGA33_002793"
     CDS             complement(729..>1110)
                     /gene="pmtD"
                     /locus_tag="PGA33_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HDG6474524.1"
                     /translation="VVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLASQKT
                     AMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDFSNWQ
                     WVISLGSIVIFFILNILYVAKKDI"
BASE COUNT          457 a          162 c          160 g          331 t
ORIGIN      
        1 ttaacaaaaa ttaacaaaga atttgcaatt aatttatttc attacctttt attactataa
       61 attggtataa tgttgtaaat gctaaaggag gtttttacaa tgacaaattt agaaacttat
      121 ttcaaaaata gccaaccctt aaatgaatac attgatggaa tgaagacaaa tcaagatacc
      181 gttctcagta tctatcaatc atttgagtta ccagaagatg attcacgcat caaaaaaata
      241 aaagagatga attattctaa agtattagta attactgaag actggtgtgg agatgcaatg
      301 atgaatctcc caattctaaa acacatcagt gaagcattaa atttagaagt acgcgtgttc
      361 catagagatg atgatacaaa actgatcgat caatatttaa caaatggtaa atcacgtgca
      421 attcctatct tcgtattctt gaatgatcaa ttcgaacaag aaacagtttg gggaccaaga
      481 gcaaatgaag tccaaaaatt tgtaactgat gttcgtgctg ataaattacc gagcaaagat
      541 catccagact atgcagattt agaaaaagaa acacacttag ttatatcaaa ccgctataaa
      601 actgacgctt cattttggaa agcagtttat aattccatcc taaataaatt agaaaataaa
      661 taaatttaca ataagacaaa ctttgaggtt gggaaaattt aaaattccca acctcaaaat
      721 tatttttatt aaatgtcttt tttagcgaca tataagatat tcaaaatgaa gaatatcact
      781 atagaaccta atgaaattac ccattgccaa ttgctaaaat caaagttaga gctaatcatt
      841 ttatcagtta agtaagcaaa agggatataa tcaaaagcat ctaaaacttt ttcaccgtat
      901 cttgggataa atgtaataaa aggtttaata aacggtacaa tgagtactaa aaatacacct
      961 aatgaaaata tcattgctgt tttttgtgat gcaagtgtga ttaagaataa aaacaagcca
     1021 aagaatagta aaaagattag gaagaaccat aaattatcta catatttaga aaacgccaaa
     1081 ttttttccat tactagatgc ctgcacaaca
//
LOCUS       DAMQYT010000351         1095 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.359, whole genome shotgun sequence.
ACCESSION   DAMQYT010000351 DAMQYT010000000
VERSION     DAMQYT010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.359"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            462..563
                     /locus_tag="PGA33_002794"
     CDS             462..563
                     /locus_tag="PGA33_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474525.1"
                     /translation="MKFAANKLTLTIFRKFCINNEKEIWIKYEEVFL"
     gene            573..845
                     /locus_tag="PGA33_002795"
     CDS             573..845
                     /locus_tag="PGA33_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HDG6474526.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNWWVNRAYYNIY"
     gene            889..>1095
                     /locus_tag="PGA33_002796"
     CDS             889..>1095
                     /locus_tag="PGA33_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDG6474527.1"
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRIDQFAKNENVAIY"
BASE COUNT          411 a          142 c          176 g          366 t
ORIGIN      
        1 taggcgacat gttcctgcat gtcgtctttt tacattttta tagtaacata tcttttattt
       61 aattgtctat ttttattaga tgtgtaacaa aattttgaag tattttccgt atattgcctg
      121 ctaaacatgt cagatttgaa aggaaaatca tactgtggta gaaatcaatt atgattatat
      181 ggtagtactt ataaataagt gatttctatt gaatatgtaa tgaagttcat atattaatat
      241 aactttcatt gatatagaag tcgcaatgat taatttaaac aataaaaaac aaccatcatt
      301 tccaaacttt gacaacgaat gatggttaaa gaaaatctca tttagtcacc gtctttttaa
      361 cgggctcaat aggcgacatg ttcctgcata tcgtctattt atacactgca caatttaata
      421 ataatacaat tatactttga tgtccattta acataaagtg gttgaaattt gcagcaaata
      481 aattaacctt gactattttc agaaaattct gtataaataa cgaaaaagag atatggatta
      541 aatatgaaga ggtgttttta tagaagaata aaatttttgg cactatatta tatttaactt
      601 tagcacttgg attatcaaca gcagcttatg catctacaga atacgcagaa ggaggcactt
      661 ggagtcatgg tgtcggcagt aagtatgttt ggtcgtatta ttatcatggt cataaaggac
      721 atggtgcaac agctattgga aaatatagat catttagtgg ttatacaaga gctggtgtaa
      781 aagcaaaagc atcagctact aaacataatt ggtgggtcaa tagagcgtat tataacattt
      841 attaaatact aaaaaatcat agaaaataaa caacgcaaga ggtacaacat gaaatggttc
      901 aaattaatac ttgatgttac aatcttcatt ctgattgcta tattgttatt tgtttataca
      961 tataaagaaa acgaagaaat attgcctgat actaaatacc ctatagcggt aactgactgg
     1021 aataaaaaat atagtaaaaa tgagatttat aaacgtatag atcaattcgc taaaaatgag
     1081 aacgtagcaa tctat
//
LOCUS       DAMQYT010000352         1061 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.360, whole genome shotgun sequence.
ACCESSION   DAMQYT010000352 DAMQYT010000000
VERSION     DAMQYT010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.360"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            421..807
                     /locus_tag="PGA33_002797"
     CDS             421..807
                     /locus_tag="PGA33_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDG6474528.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
     gene            800..>1061
                     /locus_tag="PGA33_002798"
     CDS             800..>1061
                     /locus_tag="PGA33_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDG6474529.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAF"
BASE COUNT          366 a          127 c          191 g          377 t
ORIGIN      
        1 tttcataagc cacagctaat gtgtatttaa aaataggaat acatgagtaa aactcatgca
       61 taagaaatac taatttctaa agaaaaagta tttctttatg ttggggcccc gccaacttgc
      121 acattattgt aagctgactt accatcagct tctgtgttgg ggccccgcta taattgaaaa
      181 gtttgttata ggtgtatttt cttttggtta actattgtta atataacatt gtagatttta
      241 ggatgttgat ttttgactta gcctttgtct gcttatgtta aatgttccag ctagtcaaac
      301 attgagtttg taattattat agtaagctag tttaaatttt ttgtgattta tgttttattc
      361 ataccaattt tgtgaaatga tacagtgaca gatgtttatt gattttaaag ggtgatgatg
      421 atggctattg taaataaagt gataattgtt gaaggaaaat ctgataaaaa aagggtgcaa
      481 caggttattg cagaaccagt caatattatt tgtactcatg gaacaatgag tatagataag
      541 cttgatgata tgatagaatc actgtatgat aaacaagttt ttgtattagc cgattctgat
      601 gacgaaggag atcgaattag aaattggttt aaacgttatt tgagtgaaag tgaacatata
      661 tttattgata aaacttactg tcaagttgcg aattgcccca aacaatattt ggcgcatgta
      721 ctttcaaaac atggctttac ttgtaagaaa gaaacacctc ttttaccgaa tataaataat
      781 gaaaggttag ttttagtaaa tgaataattc attagacatc aaagatgtaa ctacatttta
      841 tgaggaagac aaacatttaa tctttggtta tacaccaacg tgtggtactt gtaaggtttc
      901 agaaagaatg ttagacattg ctaatgaaat attgcagtta ccattattga aaatagattt
      961 aaacttttat cctcagtttt gtaaagatat gcaaatcatg tctacgccga ttttattgtt
     1021 gatgaataaa gataaagaag taaaacgaat ttatgcattt a
//
LOCUS       DAMQYT010000353         1031 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.361, whole genome shotgun sequence.
ACCESSION   DAMQYT010000353 DAMQYT010000000
VERSION     DAMQYT010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.361"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..475)
                     /locus_tag="PGA33_002799"
     CDS             complement(<1..475)
                     /locus_tag="PGA33_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDG6474530.1"
                     /translation="MKIKTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVIELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDQDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHK"
     gene            complement(459..>1031)
                     /locus_tag="PGA33_002800"
     CDS             complement(459..>1031)
                     /locus_tag="PGA33_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474531.1"
                     /translation="LIGETKGKIKKFLPEEKFWILHYSFLRALILGFFIGFIPINIYN
                     DLKLNNLQFISVLTCYTVMGFVSSRYLTKYLNYKFVSEICLVIFLIIYTYQSFIAVTI
                     SMIFLGISSGLTRPQTINKLSSSSNLRVMLNYAETLYFIFNIAFLLMGGYLYTIGTIQ
                     YLILFISLLIFIYLIIIFYFTRREQHENKN"
BASE COUNT          383 a          166 c          116 g          366 t
ORIGIN      
        1 ttttatgttg atttacttct tttaaaaatt cattcaagta gaacccattc ttcaaattta
       61 aagaatgatc taacgcataa actgttaatt catattgatg atcttgatca ggtggtgtag
      121 gtcctacata gtgattttct atttctgaaa aatcttcagc caacaaccca ctagtaaagc
      181 tatttttacc ttgtacatag tcgccctttg ttcttgataa atctgctttt atagaaattg
      241 aattgccact aacacttaca ttagctacac tccaatgaat ccaagcaaag ccacatacag
      301 gaattgcatc atagtcaatt aaagaccaag caagatattt agtcccttcc ggtaattcaa
      361 ttacttcaaa tggaaatgac ttaattggat tcccgttaat agaatcactc acatctgcac
      421 ctgctgcgta ttcatatggt atattgttcc ctttaaattc agtttttatt ttcatgttgc
      481 tctctccttg taaaataaaa tattattatt aaatatataa aaattaataa cgaaataaat
      541 aatattaagt attgaatagt tcctattgta tataagtaac cacccataag taaaaatgcg
      601 atattaaaaa taaaatataa cgtttctgca taattaagca tcactcttaa gttactactg
      661 ctagaaagtt tatttatagt ttgtggacga gttaaccctg aagaaatacc taaaaatatc
      721 atagaaatag taactgctat gaaactttga tatgtatata ttattaaaaa tattactaaa
      781 caaatttctg acacaaactt ataattcaag tatttagtta aataacgtga agatacaaaa
      841 cccataactg tgtaacaagt taatactgaa ataaattgta aattattcag ttttaaatca
      901 ttatatatat taattggaat aaatcctata aaaaatccta atattaacgc tcttaaaaaa
      961 gaataatgca atatccaaaa cttctcttct ggtagaaatt tctttatctt tccctttgtt
     1021 tctcctatta a
//
LOCUS       DAMQYT010000354         1019 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.362, whole genome shotgun sequence.
ACCESSION   DAMQYT010000354 DAMQYT010000000
VERSION     DAMQYT010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.362"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            108..860
                     /gene="sarS"
                     /locus_tag="PGA33_002801"
     CDS             108..860
                     /gene="sarS"
                     /locus_tag="PGA33_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HDG6474532.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE"
BASE COUNT          408 a          148 c          123 g          340 t
ORIGIN      
        1 aaattttaat aaaaatatca tattattttt aattgtaaat tatggattat ttttcttatt
       61 atattgtgtt atattaaaat aaagtgcata tacaaggaga taaaacaatg aaatataata
      121 accatgacaa aattagagat tttataatca ttgaagcata tatgtttcgt tttaagaaaa
      181 aagtcaagcc tgaagtcgat atgactataa aagaatttat attactgact tatttatttc
      241 atcaacaaga aaacacactt ccatttaaga agattgtttc agatttatgt tataaacaat
      301 cggatttagt acagcatata aaagtacttg tgaaacattc atatattagt aaagttcgaa
      361 gtaaaattga tgagcgtaat acttacattt caatatctga agaacaacga gagaaaattg
      421 cagaacgtgt tacattgttt gatcaaatca ttaaacaatt taaccttgca gatcaaagtg
      481 aatcacagat gataccaaaa gatagtaaag aatttctaaa cttgatgatg tatacaatgt
      541 atttcaagaa tattatcaaa aaacatctaa cattaagttt tgtagaattc acaattctag
      601 ctattatcac ttctcaaaat aaaaacatcg ttcttcttaa agatttaatt gaaacaatcc
      661 accataaata ccctcaaact gttagagctc tcaataattt aaaaaagcaa ggctatctaa
      721 taaaagaacg ctcaactgaa gatgaaagaa aaattttaat tcatatggat gacgcgcagc
      781 aagaccatgc tgaacaatta ttagctcaag tgaatcaatt attagcagat aaagatcatt
      841 tacatcttgt ttttgaataa tatctctatt acgcaagtgt gctgtattct aaagtgcact
      901 tgtgttttct attttttaat aaaacctcag cacataatga acaactttct attttctata
      961 tcacttaaaa ccatttccga aattaaacct cagcacattc aaagccccac tttattctt
//
LOCUS       DAMQYT010000355         1019 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.363, whole genome shotgun sequence.
ACCESSION   DAMQYT010000355 DAMQYT010000000
VERSION     DAMQYT010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.363"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            48..>1019
                     /gene="sbi"
                     /locus_tag="PGA33_002802"
     CDS             48..>1019
                     /gene="sbi"
                     /locus_tag="PGA33_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein Sbi"
                     /protein_id="HDG6474533.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNEKDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKVESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYL"
BASE COUNT          449 a          184 c          155 g          231 t
ORIGIN      
        1 ctaaaagcat acaataaatt taatatgtaa aaagaaaggg aatacacatg aaaaataaat
       61 atatctcgaa gttgctagtt ggggcagcaa caattacgtt agctacaatg atttcaaatg
      121 gggaagcaaa agcgagtgaa aacacgcaac aaacttcaac taagcaccaa acaactcaaa
      181 acaactacgt aacagatcaa caaaaagctt tttatcaagt attacatcta aaaggtatca
      241 cagaagaaca acgtaaccaa tacatcaaaa cattacgcga acacccagaa cgtgcacaag
      301 aagtattctc tgaatcactt aaagacagca agaacccaga ccgacgtgtt gcacaacaaa
      361 acgcttttta caatgttctt aaaaatgata acttaactga acaagaaaaa aataattaca
      421 ttgcacaaat taaagaaaac cctgatagaa gccaacaagt ttgggtagaa tcagtacaat
      481 cttctaaagc taaagaacgt caaaatattg aaaatgcgga taaagcaatt aaagatttcc
      541 aagataacaa agcaccacac gataaatcag cagcatatga agctaactca aaattaccta
      601 aagatttacg tgataaaaac aaccgctttg tagaaaaagt ttcaattgaa aaagcaatcg
      661 ttcgtcatga tgagcgtgtg aaatcagcaa atgatgcaat ctcaaaatta aatgaaaaag
      721 attcaattga aaacagacgt ttagcacaac gtgaagttaa caaagcacct atggatgtaa
      781 aagagcattt acagaaacaa ttagacgcat tagttgctca aaaagatgct gaaaagaaag
      841 tggcgccaaa agttgaggct cctcaaattc aatcaccaca aattgaaaaa cctaaagtag
      901 aatcaccaaa agttgaagtc cctcaaattc aatcaccaaa agttgaggtt cctcaatcta
      961 aattattagg ttactaccaa tcattaaaag attcatttaa ctatggttac aagtattta
//
LOCUS       DAMQYT010000356         1017 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.364, whole genome shotgun sequence.
ACCESSION   DAMQYT010000356 DAMQYT010000000
VERSION     DAMQYT010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.364"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(5..913)
                     /gene="rocF"
                     /locus_tag="PGA33_002803"
     CDS             complement(5..913)
                     /gene="rocF"
                     /locus_tag="PGA33_002803"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HDG6474534.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
BASE COUNT          324 a          199 c          121 g          373 t
ORIGIN      
        1 ttatttataa taaagtttca ccaaaaaatg ttccaactaa tgaaaccgct tgttcagcag
       61 tatgattatt actgtcaatc aatggattta cttcaactaa atccattgag gaaattaaat
      121 gtgattgatg cagtaattcc aatgcaaaat ggctttctct ataactaaga ccacccaaaa
      181 ctctagtacc agtgcctggc gtttcaagcg gatctaaagc atcaacatct aaagataaat
      241 gaacgccatc aacattgcgt gacttcaaat attctattgt attttcaatt acttccttta
      301 tccccaattt atcaatatct gacatagtaa atgttttaat attatgatct ttgataaatt
      361 gtctttcacc tttatctaaa tctctcatac caattagtac gatgttttct ggcttgatta
      421 cattactatt taattctaaa agttctttgg gaccttcgcc tgtcaaaatc cttagaggca
      481 taccatgaat atttccactt ggtgactctt caggtatatt taaatcacca tgtgcatcat
      541 accaaataac acctaaatta ttataatgtt tacttattgc tgatactgaa cctaccgcaa
      601 tagaatgatc tccaccaaga actagaggaa atctgttatt ttcaattgat gctgaaacct
      661 ctttatttaa tttttgatta acatctataa tttcatcata atttcttaat cctttttgtt
      721 cactatgaaa tttttcaatg ttcacagcag gtaccttaat atcccccttg tcatatacat
      781 caaggtctaa ttgctttaat cttgaaatta atccagcata tctaattgct gttggtccta
      841 aatcaacacc taattttctt tgtccaaatg ttgatggtgc acctataata tcaattgctt
      901 ttgtctttgt cataagcgtc cccctttgct ctatgtaatt aaagaataat gtatgcgctt
      961 accattatca agcaatagct acacatataa tctgtttatc ttattacttc ataaaaa
//
LOCUS       DAMQYT010000357         1009 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.365, whole genome shotgun sequence.
ACCESSION   DAMQYT010000357 DAMQYT010000000
VERSION     DAMQYT010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.365"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..>1009)
                     /locus_tag="PGA33_002804"
     rRNA            complement(<1..>1009)
                     /locus_tag="PGA33_002804"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          230 a          298 c          196 g          285 t
ORIGIN      
        1 taaaacgatt ataggtggta caggaatatc aacctgttat ccatcgccta cgcctgtcgg
       61 cctcagctta ggacccgact aacccagagc ggacgagcct tcctctggaa accttagtca
      121 atcggtggac gggattctca cccgtctttc gctactcaca ccggcattct cacttctaag
      181 cgctccacat gtccttacga tcatgcttca acgcccttag aacgctctcc taccattgtc
      241 caaaggacaa tccacagctt cggtaatatg tttagccccg gtacattttc ggcgcagtgt
      301 cactcgacta gtgagctatt acgcactctt taaatgatgg ctgcttctaa gccaacatcc
      361 tagttgtctg ggcaacgcca catccttttc cacttaacat atattttggg accttagctg
      421 gtggtctggg ctgtttccct ttcgaacacg gaccttatca cccatgttct gactcccaag
      481 ttaaattaat tggcattcgg agtttgtctg aattcggtaa cccgagaggg gcccctcgtc
      541 caaacagtgc tctacctcca ataatcatca cttgaggcta gccctaaagc tatttcggag
      601 agaaccagct atctccaggt tcgattggaa tttctccgct accctcagtt catccgctca
      661 cttttcaacg taagtcggtt cggtcctcca ttcagtgtta cctgaacttc aacctgacca
      721 agggtagatc acctggtttc gggtctacga ccaaatacta aacgccctat tcagactcgc
      781 tttcgctacg gctccacatt tactgcttaa ccttgcatca aatcgtaact cgccggttca
      841 ttctacaaaa ggcacgccat cacccattaa cgggctctga ctacttgtaa gcacacggtt
      901 tcaggttcta tttcactccc cttccggggt gcttttcacc tttccctcac ggtactggtt
      961 cactatcggt cactagagag tatttagcct taggagatgg tcctcccag
//
LOCUS       DAMQYT010000358          998 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.366, whole genome shotgun sequence.
ACCESSION   DAMQYT010000358 DAMQYT010000000
VERSION     DAMQYT010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.366"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            132..998
                     /locus_tag="PGA33_002805"
                     /pseudo
     CDS             132..998
                     /locus_tag="PGA33_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          412 a          139 c          149 g          298 t
ORIGIN      
        1 catactttta aaaaataaga cactttgcca aacttgcaca taaatgttta attcaataat
       61 ttgaattttc tgtgttgggt cccttcgtat aatttaataa ataccactaa actaaattaa
      121 tgaagtgcct tatgtataaa aattataact tgatccaact tacactacca atagaaactt
      181 ctgttagaat tcctcaaatt gatatttcgc gatatgttaa tgaaattgtt gaaacgatac
      241 ctgatagcga attcgataaa ttcagacatc atcgtggcgc aacatcctat catccaaaaa
      301 tgatgttaaa aatcacctta tatgcatata ctcaatctgt attttctggt cgtagaatag
      361 agaaattact tcatgacagt attcgaatga tgtggttagc tcaaaatcaa acaccttctt
      421 ataaaactat taatcgtatt agagtgaatc ctaatactga tgcgttaatt gaatctttat
      481 ttatccagtt tcatagtcaa tgtttaaagc aaaatcttat tgatggtaca aaagtagaag
      541 ctaatgccaa taggtataca tttgtgtgga agaaaagtat tcaaaatcac gaatcgaaat
      601 tgaacgaaaa ttcaaaaaca ttatatcgtg acttagttga agaaaaaata ataccagaga
      661 taaaagaaga tggagatagc gatttaacaa tagaagaaat agatttaatt ggtagtcatt
      721 tagataaaga aatcgaaggt ttaaatcatt ctattgagaa cgaagatagt actcaaatta
      781 gaaaacagac ccgtaaaaaa gaactaagat taagaagttc aaaaataaat ttgatgatta
      841 ttccgaaaga aaaaataaat atgaagaaca aaaatcgatt cttaaagata gaaatagctt
      901 ttctaaaact gatcatgatg caacttttat gagaatgaag gaagaccata tgaaaaatgg
      961 ccaacttaag ccaggataca atttacaaat agcgacaa
//
LOCUS       DAMQYT010000359          992 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.367, whole genome shotgun sequence.
ACCESSION   DAMQYT010000359 DAMQYT010000000
VERSION     DAMQYT010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.367"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..163
                     /locus_tag="PGA33_002806"
     CDS             <1..163
                     /locus_tag="PGA33_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HDG6474535.1"
                     /translation="YGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
     gene            complement(248..349)
                     /locus_tag="PGA33_002807"
     CDS             complement(248..349)
                     /locus_tag="PGA33_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474536.1"
                     /translation="MENFDVGGGNFTFMEIGIGFMTHKPKRLNDDID"
     gene            427..>992
                     /locus_tag="PGA33_002808"
     CDS             427..>992
                     /locus_tag="PGA33_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HDG6474537.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPEDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFL"
BASE COUNT          365 a          156 c          191 g          280 t
ORIGIN      
        1 atacggactt tataaaacaa tctcaaaaga tggtagggtc aaaattagct tgaaagatgg
       61 cagtttttat aaccttgatt taagatctaa attaaaattt aaatatatgg gggaagtcat
      121 agaaagcaaa caaattaaag atattgaagt taacttaaag taaatcatta cgaataatta
      181 aaagtaattg aagcggctta acggtgaaat gtaaattggt gcgcatagct tatacaaaaa
      241 ggatgcatca atcgatatcg tcgttaagcc gttttggttt gtgtgtcatg aatcctatcc
      301 caatctccat aaaggtaaaa tttccaccac caacatcaaa attctccaca tcgcaacata
      361 accaaatgtt ataataaatc tattacacaa agagataaat tacttattca aaggcggagg
      421 aatcacatgt ctattactga aaaacaacgt cagcaacaag ctgaattaca taaaaaatta
      481 tggtcgattg cgaatgattt aagagggaat atggatgcga gtgaattccg taattacatt
      541 ttaggcttga ttttctatcg cttcttatct gaaaaagcgg aacaagaata tgcagatgcc
      601 ttgtcaggtg aagacatcac gtatcaagaa gcatgggcag acgaagaata ccgtgaagac
      661 ttaaaagcag aattaattga ccaagtcggt tacttcattg agccagaaga tttattcagt
      721 gcgatgattc gtgaaattga aacgcaagat ttcgatatcg aacacctggc gacggcaatt
      781 cgtaaagttg aaacatcaac attaggtgaa gaaagtgaaa atgactttat cggtctgttc
      841 agcgatatgg atttgagttc aacgcgacta ggtaacaatg tcaaagaacg tactgcttta
      901 atctctaaag tcatggttaa tcttgacgac ttaccattcg ttcacagtga catggaaatt
      961 gatatgttag gtgatgcata tgaattccta at
//
LOCUS       DAMQYT010000360          987 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.368, whole genome shotgun sequence.
ACCESSION   DAMQYT010000360 DAMQYT010000000
VERSION     DAMQYT010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.368"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(1..752)
                     /locus_tag="PGA33_002809"
                     /pseudo
     CDS             complement(1..752)
                     /locus_tag="PGA33_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829558.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
BASE COUNT          370 a          177 c          117 g          323 t
ORIGIN      
        1 atttccccat tcctcttctt cgtgagttaa taaatgaagc attgctcgtt cttgtatatt
       61 ttcaatcatt tttaaattcg gttttaaaat atgcaaatca tcaaaacaat ctttccaaca
      121 atcaaccata tctcgtttta attcaatttc cacacgccat agaaatgttg aatcaatttc
      181 aacatctgca ttatctttac gttcttgttt ttattataaa tccgaataaa cctatcacta
      241 ttacgcacac caaaatattt tgtttctggt tttacattac gtccataaaa tatagttttc
      301 tttaccgact tatctgacaa tgcataatag tcatttaaat caaattcaaa atcaaaagcc
      361 aaatctaatc tcgtaaaact aacatcgtcc aaataactga tgatattttg ttttaaccaa
      421 agcacttcat catgcgaaag cttattagga ttaaattcaa cgcgcatact acgtctattc
      481 caaagagttg cttttatttt gtcatattca atataaactt tttctttaag agctttagct
      541 ttaaagtttg tttgtaaaat atcccaaagc cgaatttcag gattagtact cataaaatgt
      601 gaaagtctct ctgcgttaga catgctaaga ttcccaacaa tcgttatagc gtcaaaagac
      661 aattttggaa tagctagtga catcctatgt cgatttaacc ggctattacc ggatattaga
      721 gtatccagtt ttacaaatgg atgaaacgaa attcaaaaca ctaaaaaata tgttccacta
      781 acagcaaaaa aataccatta tgttcctact aaaaaactaa aaatactgga gaacaaatgt
      841 caggatataa cttaggatac tatgtaataa aaatttacaa taaaaaaaca ggaaaacaaa
      901 tttcaagtaa aagaataccc atacaaagag gataaaataa aaaacctcga actgaaatga
      961 tgatcttttc agctcgaggt ttaaata
//
LOCUS       DAMQYT010000361          983 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.369, whole genome shotgun sequence.
ACCESSION   DAMQYT010000361 DAMQYT010000000
VERSION     DAMQYT010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.369"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            54..>983
                     /locus_tag="PGA33_002810"
     CDS             54..>983
                     /locus_tag="PGA33_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="involved in the sodium dependent uptake of proline;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proline symporter"
                     /protein_id="HDG6474538.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIIRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAY"
BASE COUNT          273 a          140 c          197 g          373 t
ORIGIN      
        1 gtataatgtt ttcaagtaac taaattataa attaaataaa gggagtgttt atcatgctta
       61 caatggggac agcattaagt caacaagtag atgccaattg gcaaacttat attatgattg
      121 ccgtctactt cttgatacta atcgttattg gcttttacgg ttacaagcaa gcaactggta
      181 acctaagcga gtacatgtta ggtggacgta gtattggacc gtatattact gcattatcag
      241 ctggagcttc agatatgagt ggatggatga ttatggggct acctggttct gtctatagca
      301 ctggtctatc agctatgtgg attacaatcg gtttaacatt aggtgcttat ataaattact
      361 ttgttgttgc tcctagactt cgtgtttata ccgaattagc tggagatgca attacattac
      421 cagatttctt taaaaatcgt ttaaacgata aaaataatgt gttaaagatt atttctggat
      481 tgattatcgt agtattcttt acattatata cacattctgg tttcgtatct ggtggtaaac
      541 tatttgaaag tgcttttgga ttagattatc atttcggttt aatattagtt gctttcattg
      601 tcattttcta tactttcttt ggtggatatt tagctgtatc aattacagat ttcttccaag
      661 gtgtcattat gttaattgcg atggttatgg tccctattgt tgctatgatg aatttaaacg
      721 gctggggaac gtttcatgat gtagcagcta tgaaacctac aaatttaaat ttatttaaag
      781 ggttatcatt tataggaatt atctctctat tttcatgggg attaggttat ttcggtcaac
      841 ctcatatcat tataaggttt atgtctatta aatcacacaa gatgctacct aaagctagac
      901 gtttaggtat tagctggatg gctgttggtt tattaggcgc tgtggctgtt ggtttaacag
      961 gtattgcatt cgtacctgct tat
//
LOCUS       DAMQYT010000362          978 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.370, whole genome shotgun sequence.
ACCESSION   DAMQYT010000362 DAMQYT010000000
VERSION     DAMQYT010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.370"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..969
                     /locus_tag="PGA33_002811"
     CDS             <1..969
                     /locus_tag="PGA33_002811"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDG6474539.1"
                     /translation="GKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLR
                     VGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAV
                     IANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPH
                     GVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLF
                     IDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRY
                     VDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE"
BASE COUNT          350 a          173 c          198 g          257 t
ORIGIN      
        1 ggtaaaaaag caggcgagtt ctatacacca caacaagtat ctaagatact ggcgaagatt
       61 gtcacagacg gtaaagataa attacgtcac gtgtatgacc caacatgtgg ttcaggttca
      121 ctgttgttac gtgttggtaa agaaacacaa gtgtatcgtt atttcggtca agaacgtaac
      181 aatactacat acaacttagc acgcatgaat atgttattac atgatgtgcg ttatgagaac
      241 ttcgatatcc gtaatgatga cacattggaa aacccagcct ttttaggcaa tacatttgat
      301 gcggttattg cgaacccacc gtatagtgcg aaatggactg cagattcaaa gtttgaaaat
      361 gacgaacgat tcagtggtta cggcaaactt gcgcctaagt ctaaagcaga ctttgccttt
      421 attcaacaca tggtacatta cctagacgat gaaggtacca tggccgttgt actcccacat
      481 ggtgtattat tccgaggtgc tgcagaaggt gtcattcgtc gttatttaat tgaagaaaag
      541 aactacttag aagctgtgat tggtttgcca gcgaatattt tctatgggac aagtattcca
      601 acatgtattt tagtatttaa aaaatgtcgc caacaagacg acaacgtact atttatcgat
      661 gcatccaatg attttgaaaa aggaaaaaat caaaatcatt taagcgatgc ccaagtcgaa
      721 cgtattatag acacatataa gcgtaaggaa acaattgata aatatagcta cagcgcgaca
      781 ctacaagaga ttgccgataa cgattacaac ctaaatatac cgagatatgt cgatacattc
      841 gaagaagaag caccgattga tttagatcaa gtccaacaag atttgaaaaa tatcgataaa
      901 gaaatcgcag aaattgagca agaaatcaat gcatacctga aagaacttgg ggtgttgaaa
      961 gatgagtaat acacaaaa
//
LOCUS       DAMQYT010000363          978 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.371, whole genome shotgun sequence.
ACCESSION   DAMQYT010000363 DAMQYT010000000
VERSION     DAMQYT010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.371"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(354..562)
                     /locus_tag="PGA33_002812"
                     /pseudo
     CDS             complement(354..562)
                     /locus_tag="PGA33_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          323 a          126 c          145 g          384 t
ORIGIN      
        1 tttttttcta taaatttagt ttcattatct tcaaattctt taatcaaatg tgaatatgtt
       61 ttaagcgttg tatgtacatc tgcatgacct aatcgttttg aaatgtaatg tttagctgtt
      121 acttaaagaa aatcaaatat tatatatacg cttgaaaaaa taaaataata atattataat
      181 ataatcaatc ggcatctacg tgcggattat aaagtaaagc gtagcataac caggagttag
      241 tcgattttta ataaattcga ctaaattatt aataaaggct aaagtaatta gtttacttta
      301 gcctttattg catttgtaat ttatagaact gcgaacaggt gactactgtt aatttatgtg
      361 attaaagttt caatgatatt tgatttatta gaccatttca aaatcgcaag gttctcatgt
      421 tctgctacag tttgcgatct ttggaagata tatcatttac tattattaac atgatatatt
      481 ctaatttgtt tgtggatggg ctgtttggtt tgatatcgcg gtaaatataa gcatcattag
      541 ttatattgtt gaaatctaag ttgaatttat tatcatacag caatgtgcca attagggaca
      601 tttttaattt tgaactctac attttgttga gagtatctgg ttatatttgc aaaatggtat
      661 gaacatttat catcacttat taagttgtct tgatgattga atgacccagt atttattcag
      721 ttcgagaaga ttgagagtgt taaatcgtat tcgagtataa tttcgattct agtcatacca
      781 ttttcataaa gtttagccat ttgtaactta aactatgaac taaataatct tctgactctt
      841 gtcaaaataa aatcgcctac ccaaatagtt taacaatatc tattctcata gaatttgttc
      901 aactaagtgt agacgattct gcattatcaa taaggctatt caatttgcta tcttaagctg
      961 tttcgaaatt ttcaaatt
//
LOCUS       DAMQYT010000364          969 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.372, whole genome shotgun sequence.
ACCESSION   DAMQYT010000364 DAMQYT010000000
VERSION     DAMQYT010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.372"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..728
                     /locus_tag="PGA33_002813"
     CDS             <1..728
                     /locus_tag="PGA33_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDG6474540.1"
                     /translation="NDLEIFITVCEEASINKAAIKLRYAQSNISQRISKLENELGVVL
                     LFRNQKGAKATKAGEEFLAYSKKVLRDTETIKNKMKNNTMSILCSELLFNYLSESEEI
                     MMSNNSINFISSGNIRKAIEKNNYDKVISFIKINDSNYRLSNVDTMKVTLYSNGSNYD
                     KEALLINKDEFCPLRKITLDNKLDSQRVMEIDSLAAIINLVKQGKGKALLPMTFENKR
                     DIVQDISKIFEVNYYTYNHIMHH"
BASE COUNT          387 a          107 c          158 g          317 t
ORIGIN      
        1 ttaatgattt ggaaattttt ataactgtat gtgaagaagc atctatcaat aaagctgcaa
       61 ttaaacttag atatgcacaa tctaatatat ctcaaagaat tagcaagctt gaaaatgaat
      121 taggtgtagt tttgcttttt agaaatcaaa aaggtgctaa ggcaactaaa gcaggcgaag
      181 aattcttagc gtatagcaaa aaagtattaa gagatacaga gactataaaa aataaaatga
      241 aaaataatac tatgtctatt ttatgctcag aactgttatt taattattta tctgagagcg
      301 aagaaattat gatgtcgaat aactcaatta attttatttc tagtggaaat attagaaaag
      361 ctatagaaaa aaataattat gataaggtta tttcattcat aaaaattaac gactcaaatt
      421 atagacttag taatgttgat actatgaaag taacgcttta cagtaatgga agtaattatg
      481 ataaagaggc tttactaata aataaagatg agttttgtcc tttaaggaaa ataactttag
      541 ataataagct tgattcacaa cgggtaatgg aaattgattc attggcagca ataataaact
      601 tagttaaaca aggaaaagga aaggctttat tacctatgac ttttgagaat aaaagagata
      661 tagtacagga catttctaag atatttgaag ttaactacta tacttataat catattatgc
      721 atcattaatt gaaagttgat tgagtttaaa ttacaggaac gattttaaaa gtctagtagt
      781 tagaatgagc gatgtgacag aaaagaaatt atgcctttta ttttgtaaat tcgttgtttt
      841 aaagtaaagg tccttgtagt ataggaaaaa gtggatgact tctccgcctt taccaccctt
      901 tcaagcataa tgataactgc taaatattga ttatagcacc attgcaaaac taatcattaa
      961 ttgtcatat
//
LOCUS       DAMQYT010000365          963 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.373, whole genome shotgun sequence.
ACCESSION   DAMQYT010000365 DAMQYT010000000
VERSION     DAMQYT010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.373"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..148)
                     /locus_tag="PGA33_002814"
     CDS             complement(<1..148)
                     /locus_tag="PGA33_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HDG6474541.1"
                     /translation="MTFEEKLSQMYNKIASEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKP"
     gene            complement(160..660)
                     /locus_tag="PGA33_002815"
     CDS             complement(160..660)
                     /locus_tag="PGA33_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDG6474542.1"
                     /translation="MTFEEKLSEIYNKIANEISGMIPVEWDQVFTIAYVNDRGGEIVF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEEDLEPWTSCEF
                     DFTRDGKLNVVFDYVDWMNSEFGPIAKENYYMYKKFGVLPETEYEINKVKEIDQYVKE
                     QDEAEL"
BASE COUNT          306 a          190 c           93 g          374 t
ORIGIN      
        1 ctggtttagt ataattaaaa atgacttccc cagcttgatc agttacataa gctattgtaa
       61 atacttgctc ccattctact ggtatcattc cactgatctc acttgcaatt ttattgtaca
      121 tttgacttag tttctcttcg aaagtcatgt tatctccccc tatagttcag cttcatcttg
      181 ctctttaaca tattgatcga tttctttaac tttattaatt tcatattccg tttctggtaa
      241 aacaccaaac tttttgtaca tataataatt ttcttttgct attggaccaa attctgaatt
      301 catccaatca acataatcaa atacaacatt caatttacca tctcttgtaa agtcaaattc
      361 acatgatgtc catggttcta aatcttcttc tttaaatgcg tttcttaact tcttaaacaa
      421 tctatataaa tccgtccaca aatcataaaa tactttttca gagacattat actctctagg
      481 gatatatgtg taataattca attcatcgct acctggttta gtataattaa aaacgatctc
      541 tccacctcta tcatttacat aagctatcgt aaatacttgg tcccattcta ctggtatcat
      601 cccactaatc tcattcgcaa tcttgttgta tatttcactt agtttttctt cgaaagtcat
      661 gttatcgccc ctatattttc aaaatagata ttggtttcat ttccgttaaa taaaaagcac
      721 taaacaacac ctctacgtcg cttaatgctt cttcaaatta atattactca ttttaaacct
      781 aaatacacta atggaactaa aataactgca actattgtta cgataactgg aattctaaat
      841 aatttcgagt ctttgcagta ttatattatt tgtaaataat agccctaaaa ctcctactca
      901 ttattttgtc tttctattta tttaactatt tataattata gcaaaaaaga tattaatagt
      961 ata
//
LOCUS       DAMQYT010000366          962 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.374, whole genome shotgun sequence.
ACCESSION   DAMQYT010000366 DAMQYT010000000
VERSION     DAMQYT010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.374"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            42..680
                     /locus_tag="PGA33_002816"
     CDS             42..680
                     /locus_tag="PGA33_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDG6474543.1"
                     /translation="MYKINIIRSDSVYNNILKAPINDRDSIFTKEILVPFKKKFEVQH
                     MPIYNDDKQTMSAIQFLDAFQISPKDLRMSDQMSIQYLNNDFWSNCEKYLKVAIDQFS
                     NYSISSQVSNYHFTVLLGDRQKPLMYLNKNRGGDGGIPGYIMIYLVPSTSTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTN"
     gene            699..956
                     /locus_tag="PGA33_002817"
     CDS             699..956
                     /locus_tag="PGA33_002817"
                     /inference="COORDINATES: protein motif:HMM:NF021537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474544.1"
                     /translation="MKNTIYNHLHLKHIFESMPYLYGDDINKLQGRPIVGLSHAAGYA
                     CGYHLVKYFLQKTNIPIEVATTLPAQKIINEVTEFWHTHTL"
BASE COUNT          349 a          146 c          155 g          312 t
ORIGIN      
        1 gttacgttat cctttatgct gacaacataa ggaggtttat catgtataaa atcaatatta
       61 ttagatcaga ttccgtctat aataatatac tcaaggcacc aataaacgac cgtgattcaa
      121 ttttcactaa agaaatatta gtacctttta aaaagaaatt tgaggtacaa catatgccta
      181 tttataatga tgacaaacag acaatgagtg cgattcaatt tttagatgcc tttcaaattt
      241 ctcccaaaga tttacgcatg tctgaccaaa tgtcaataca atacctgaat aatgatttct
      301 ggagtaattg cgaaaaatat ttaaaagttg ctatagatca attttcaaat tatagtatct
      361 catctcaagt ttctaactat catttcacag tattacttgg agatcgccaa aaaccactta
      421 tgtatctaaa taaaaatcgc ggtggtgatg gtggcatacc aggttatatt atgatttatt
      481 tagtgccgag tacaagtaca attaattcta tgaaaagctt aattgcacat gaagtaaatc
      541 ataatatgcg ctatcaatat attgattggg atggcggaag tttgattgaa ttgattattg
      601 cagaaggatt agctgaaaac tatgtagaat cattgtatgg taaagcgcat attggaccgt
      661 gggtaacaaa tactaattga agtcgtgata acgtaaaaat taaaaatact atttataatc
      721 atttacattt aaagcatata tttgaatcga tgccttatct ctatggtgat gatattaata
      781 aacttcaagg taggcctatc gttggcttat ctcatgctgc cgggtatgca tgtggctatc
      841 acttggtaaa atacttttta caaaaaacaa acatacctat tgaagttgct acaacacttc
      901 cagcacaaaa aataataaat gaggtgactg aattttggca tacacataca ctttaaaaga
      961 ta
//
LOCUS       DAMQYT010000367          957 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.375, whole genome shotgun sequence.
ACCESSION   DAMQYT010000367 DAMQYT010000000
VERSION     DAMQYT010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.375"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..>957)
                     /locus_tag="PGA33_002818"
     CDS             complement(<1..>957)
                     /locus_tag="PGA33_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDG6474545.1"
                     /translation="FPGFEGEWEEKQLGDLTDRVIRKNKNLESKKPLTISGQLGLIDQ
                     TEYFSKSVSSKNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRYDSGVLSSLYICFS
                     IKSEMSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFFTILIKYPSLEEQQ
                     KIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEDYPDWENSKIE
                     KYLKERNERSDKGQMLSVTINSGIIKFSELDRKDNSSKDKSNYKVVRKNDIAYNSMRM
                     WQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIH"
BASE COUNT          280 a          183 c          113 g          381 t
ORIGIN      
        1 atgaatcatt ctatgtgttt taaacttata tccaataaat aatgagctag tattttgtgt
       61 tggataaagc acagtatatg cagggctaac aatcccatta taatttgatt taccactagc
      121 cccttgccac attctcatag aattatatgc aatatcattt ttcctaacta ctttataatt
      181 acttttatct ttacttgaat tatcttttct atccaattca ctaaatttta taatgccact
      241 atttatagtt actgaaagca tttgcccttt gtcagaacgt tcgtttctct cttttaaata
      301 tttttctatt ttgctatttt cccaatctgg ataatcttca ccattctcat ctttgaatcg
      361 cagttcctgt gagaaaattt tctgcatata gccttttttc tgttgttgaa gtaattcaag
      421 cttttgttct tctaattcaa tttgtcggtc gagtttgctg aagaacttgc ctattttttg
      481 ctgttcttct aaacttggat atttaattag aatagtaaaa aaatcattca cagaaacatt
      541 taataatccg tgatttcttg caccctcaac tgcaattcca gaaacttctc tataccagtg
      601 tgtcgaatca aaatatgctt ccatgaagtc tttagacatt tcacttttaa tagaaaaaca
      661 aatatacaaa gaggacaata caccactatc atatctagtt aatcttttaa tagcccctaa
      721 tgggtatcca ttagaataac ttttgttata cgcgaattct ccattcttta ttagtgtata
      781 attttctaga tttttcgacg aaactgattt actaaaatat tctgtttgat caattaaacc
      841 taactgtccg gatattgtta aaggcttttt cgattctaag tttttatttt tcctaattac
      901 tctatctgta agatccccta actgcttctc ttcccattcg ccttcaaacc ctgggaa
//
LOCUS       DAMQYT010000368          947 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.376, whole genome shotgun sequence.
ACCESSION   DAMQYT010000368 DAMQYT010000000
VERSION     DAMQYT010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.376"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..851
                     /locus_tag="PGA33_002819"
     CDS             <1..851
                     /locus_tag="PGA33_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6474546.1"
                     /translation="KNISFDIEQGKCIALIGKNGAGKSTLIDILIGNVNANSGEIFDK
                     DKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENPLPLEEIIELTKFDSSQLNQF
                     VNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEIREYFWSIIENLKEDNRTILY
                     TSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQSQITLSDEYIRKLKLDKDDLV
                     IQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKVSIVDSYFNNKKQRGSNYDTK
                     LLENRI"
     gene            820..>947
                     /locus_tag="PGA33_002820"
     CDS             820..>947
                     /locus_tag="PGA33_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6474547.1"
                     /translation="MILSYLKIEFKVIMRKKTTLILSILFPVIFYILFTSILELPE"
BASE COUNT          379 a          121 c          133 g          314 t
ORIGIN      
        1 taaaaaatat ttcgttcgat attgaacaag gtaaatgtat cgctttaatt ggaaaaaatg
       61 gtgctggaaa gtcaacgtta attgatatat taattggtaa tgttaatgct aattctggtg
      121 agatatttga taaagacaag ttattacaaa gtgaaaatcg cagtataatg ttccaaaaaa
      181 cgatgtttcc agatcaatta aaagttattg agattatcaa cttatatcaa tcattttacg
      241 aaaatccatt accattggaa gaaataatag aactgacgaa atttgattct agtcaactga
      301 accaatttgt aaataaactt tctggtggtc aacaacgatt actcgatttt gtattatctt
      361 taatcggaca accacaattg atcttattag atgaaccaac atcgactatg gatatagaaa
      421 ttagagaata tttttggtca attattgaaa atttaaaaga agataatcga acgatactct
      481 atacatcgca ctatattgaa gaagtcgaac gtatgtcaga caaaattatt ctcattgaaa
      541 atggagaaat aatacttaat gattcaacgt cacatattag aaccaatcag caatctcaga
      601 ttacgttatc cgatgaatat ataagaaagt taaaactaga taaagatgat ttagttattc
      661 aaaaaaatca taatggcact atcaaaatta ttacttcaaa tgtaaatgat acgattttat
      721 atcttcaaca acttcatatt aatttggatg atattgaaat acaaaaagtc tcaattgttg
      781 attcatactt caacaataaa aagcaaaggg gatctaatta tgatactaag ttacttgaaa
      841 atcgaattta aagttataat gcgtaaaaaa acaacattaa tattatctat tttatttcct
      901 gttatattct atatattatt tacttcgata ttggaattgc cggaaga
//
LOCUS       DAMQYT010000369          927 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.377, whole genome shotgun sequence.
ACCESSION   DAMQYT010000369 DAMQYT010000000
VERSION     DAMQYT010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.377"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            54..554
                     /locus_tag="PGA33_002821"
     CDS             54..554
                     /locus_tag="PGA33_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDG6474548.1"
                     /translation="MNFEEKLSEMYNKIANEINEMIPVEWEKVYVIAYVDDGGGQVIF
                     YYTKPRNDELYYYSSIVEDYNVLEEIFDDLWMELYRSFKKLRNIFKEESLEPWTSCEF
                     DFTKEDKLKVSFDYIDWKNTEFDQLGQENYYNYRKFGIIPEMEYEMEEVKQIEQYIKE
                     QEEAEQ"
     gene            565..927
                     /locus_tag="PGA33_002822"
                     /pseudo
     CDS             565..927
                     /locus_tag="PGA33_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498862.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
BASE COUNT          384 a           84 c          188 g          271 t
ORIGIN      
        1 aatcgagcaa tatgttaaag agcaagaaga agctgaacaa tagaggcgat aacatgaatt
       61 tcgaagaaaa attaagtgaa atgtacaata aaattgcaaa tgagatcaat gaaatgatac
      121 cagtagagtg ggaaaaggta tatgtaattg catatgtgga cgatggaggt gggcaagtta
      181 tattttatta taccaagccc agaaatgatg agctgtatta ttattcgagt atcgttgaag
      241 attataatgt tttagaagaa atctttgatg atttatggat ggaactttat agatcattta
      301 aaaaattaag aaatatattt aaagaagaaa gtcttgaacc atggacatca tgtgaatttg
      361 actttacaaa agaagataaa ttaaaagttt catttgatta tattgattgg aagaatacag
      421 aatttgatca attaggccaa gaaaactact ataactatag aaaattcgga attataccag
      481 aaatggaata tgaaatggaa gaggttaaac aaatcgagca atatattaaa gagcaagaag
      541 aagctgaaca atagaggcga taacatgatt ttcgaagaaa aactaaatga aatgtacaac
      601 gagattgcga ataaaattag tagcatgata ccagtagaat gggaaaaggt atatacaatg
      661 gcttatatag atgatggagg aggtgaagta ttctttaatt atactaaaca gcgatgaatt
      721 gaattattac accgatatac ctaaggagta taacatttct gtgcaagtat ttgatgattt
      781 atggatggat ttatatgatt tgtttgagga attaagaaat ttatttaaag aagaaggact
      841 agaaccatgg acatcatgcg aatttgattt tacaagagaa ggtaaattaa aagtttcatt
      901 tgattatatt gattggataa attcaga
//
LOCUS       DAMQYT010000370          925 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.378, whole genome shotgun sequence.
ACCESSION   DAMQYT010000370 DAMQYT010000000
VERSION     DAMQYT010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.378"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            26..565
                     /locus_tag="PGA33_002823"
     CDS             26..565
                     /locus_tag="PGA33_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474549.1"
                     /translation="MVAYASHLSFLLFSNKKVLKINAYTGTDMAKLDLNSLDDEHVKL
                     LINELKYPETHIDVNELKTIVASRINERQEIISFKLGIKYLLTIKRGNIEKDRFSISI
                     IFKDTYHTLVRIDINGGTHDNPDGTIAPKSHIHIYNDKYDKKDRFAYEINLKDFPDIY
                     NLYNVYMSFLEYNNIKDLE"
     gene            578..>925
                     /locus_tag="PGA33_002824"
     CDS             578..>925
                     /locus_tag="PGA33_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HDG6474550.1"
                     /translation="MTTFDAKKLKKEYLDWYNQTLEFSNLSNNVVRIDTPFKDNSLDN
                     LIIYALYDQSRDMITLTDDGYTIFDLENNGISLNKSKKRKKIFEEHLSAYGIKYNDKT
                     HEIFVQTNFKNFNK"
BASE COUNT          378 a          115 c          134 g          298 t
ORIGIN      
        1 ggtagtaaat tgatattata cacttttggt tgcgtatgca tctcacctat cttttttgct
       61 tttttcaaat aagaaagtat taaaaattaa tgcctataca ggaacagaca tggctaaact
      121 tgatttaaat agtcttgacg acgagcacgt aaaattatta ataaatgaat taaaatatcc
      181 agaaactcat atcgatgtaa atgaattaaa aacaatagtt gctagtcgaa taaatgaaag
      241 gcaagaaata ataagtttta agttaggaat aaagtactta ttaacaataa aaagagggaa
      301 catagaaaaa gataggtttt caatttcaat cattttcaaa gatacctatc acaccctagt
      361 tagaatagat attaacggtg gtactcacga taatccagat ggaacaatcg ctccgaaaag
      421 tcatattcac atatataatg ataagtatga taaaaaggat aggtttgctt atgaaattaa
      481 cttgaaagat ttccccgaca tctataactt gtataatgtg tatatgtcgt ttttagagta
      541 taataatata aaagaccttg aataaaggag ggataatatg actacatttg atgctaaaaa
      601 attaaaaaaa gaatatcttg attggtataa tcagacctta gagttttcta atttatcaaa
      661 caatgtagta agaatagata ctccttttaa agataattct ttagataatt taataattta
      721 cgctttatac gatcagtcca gagacatgat tacactgaca gatgacggct atactatatt
      781 tgatttagaa aataatggta tttctttaaa taaatcaaaa aaacgtaaaa agatttttga
      841 agagcacctt tcagcttacg gtattaaata taacgataaa actcacgaaa tttttgttca
      901 aactaacttt aaaaatttta ataaa
//
LOCUS       DAMQYT010000371          923 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.379, whole genome shotgun sequence.
ACCESSION   DAMQYT010000371 DAMQYT010000000
VERSION     DAMQYT010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.379"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..824
                     /locus_tag="PGA33_002825"
     CDS             <1..824
                     /locus_tag="PGA33_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HDG6474551.1"
                     /translation="KNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKMLNEVK
                     DITEDDIDTSLRILDFSFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNMFL
                     EDLIELSKYNNEKYQKGKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPI
                     FITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLESKEVQKILSHRAKGIKMYIFV
                     QKKDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN"
BASE COUNT          394 a           87 c          153 g          289 t
ORIGIN      
        1 ctaaaaatct ggttttcttt tcaaggcaaa tagcacctgg attgaaaaga atcgatagtt
       61 tagttttaga agaactttta aaaaatgaat taacatatga tgaattaaaa aataaaatgt
      121 tgaacgaagt taaggatata acagaagatg atatagatac ttcattaaga attttagatt
      181 tttcatttta caacgcagga attgaaaaaa tctatggaag tcccattata gaacgtaatg
      241 aaagaatgat aagactaagc gatgcattta ctaatgctct aagtaatcaa acatttaata
      301 tgtttttaga agatcttatt gaattatcaa agtataacaa tgaaaaatac caaaaaggta
      361 aaaatggact gattttatat aataaatatt caagagaaga ttttagtaaa atatttaatt
      421 ggaataaaaa tggttcgtct gtaatcatgg gatacatgat aaaaagccaa gaaatgccaa
      481 tatttatcac ttatgataag catgaggata taagtgatag cacaaaatat gaagatgaat
      541 ttttaagtca agatgaatta aaatggttta ctaaatctaa tagaacgcta gaatcaaaag
      601 aagttcagaa aattttgtcc catagagcaa aaggaattaa aatgtatatt tttgtacaga
      661 aaaaagatga tgatggtata tatttttatt atttaggaac tgctggatat atagaaggtt
      721 cagaaaagca agataaaatg ccgaatggtt caaacgtagt aacgatggat ttagcactgg
      781 ataaagctgt aagagatgat atatatcgtt atctaacaaa ttaatgtaaa ccgaaaaatg
      841 aatgttagta aagttgtatt taaacatcaa ttttaataag taaatacatt gagtttcagt
      901 gttattattt taaatataag cgc
//
LOCUS       DAMQYT010000372          920 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.380, whole genome shotgun sequence.
ACCESSION   DAMQYT010000372 DAMQYT010000000
VERSION     DAMQYT010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.380"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..497
                     /locus_tag="PGA33_002826"
     CDS             <1..497
                     /locus_tag="PGA33_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474552.1"
                     /translation="RDEPAPPLQNDNDLRRRQQDKPMPLFINKDNQKNGNKRREQQES
                     MNGNDVKSASVESNANNYSKQPQKASQQEHQVRETRQRKDIQRSPQVVNQPLNNENHS
                     INRKEQKSVQTAYDTDVQKRQIQNATQNQQSRQSGNRNQPITRNSQSKDRLKEQKDIN
                     KHGK"
     gene            487..>920
                     /locus_tag="PGA33_002827"
     CDS             487..>920
                     /locus_tag="PGA33_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDG6474553.1"
                     /translation="MVNKKDIAKKVIGKTPIGVKLKLAKVIIILCVVAFFTFPVIVMF
                     LLAPDSNKDKDDAGCTVSGGNVSANGIDKFNENAKGGKLEGKGKEIQKIAEKNKVPVN
                     IFMAIIASESQWGKGENATRQNNPLSVMGSKSIHDSTYPTIE"
BASE COUNT          386 a          130 c          172 g          232 t
ORIGIN      
        1 aaagagatga acccgcccca ccacttcaaa atgataatga tttaagaaga aggcagcagg
       61 ataaaccaat gcctttattt ataaataaag ataatcaaaa aaatggtaat aagcgaagag
      121 aacaacaaga gtcaatgaat ggtaacgatg taaaatctgc ttctgttgaa tcaaatgcaa
      181 ataattattc taaacaacca caaaaagcat cacagcaaga gcatcaagta cgagaaacaa
      241 gacagcgaaa agatatacaa aggtcgccac aagtggttaa tcagccatta aataatgaga
      301 atcattcaat aaatcgtaaa gaacagaagt cagttcaaac tgcttatgat actgatgttc
      361 aaaaacgaca aatacaaaat gcaacacaga atcaacaaag tagacaatct ggaaatcgta
      421 atcaacctat tacacgtaat tctcagtcaa aagaccgtct aaaagagcaa aaggatatca
      481 ataagcatgg taaataaaaa agatattgct aaaaaagtaa taggtaaaac gccaataggt
      541 gttaaattga aattagcgaa agtcatcatc attttgtgtg tggtggcttt ttttacattt
      601 ccagtaattg taatgttttt gttagcgcct gattcaaata aagataaaga tgatgctggt
      661 tgtactgtta gtggcggtaa tgtttcggca aatggtattg ataagtttaa tgaaaatgct
      721 aaaggtggaa aattagaggg taaaggaaaa gaaatacaga aaattgcaga aaaaaataaa
      781 gtacctgtga atatttttat ggctattatt gcgagtgaat cgcaatgggg taaaggagaa
      841 aatgcgacaa gacaaaataa tcctttgtct gttatgggtt caaaatcgat tcatgattca
      901 acatatccaa ctattgaaga
//
LOCUS       DAMQYT010000373          906 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.381, whole genome shotgun sequence.
ACCESSION   DAMQYT010000373 DAMQYT010000000
VERSION     DAMQYT010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.381"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            125..>906
                     /gene="coa"
                     /locus_tag="PGA33_002828"
     CDS             125..>906
                     /gene="coa"
                     /locus_tag="PGA33_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Staphylocoagulase, like its paralog von Willebrand
                     factor binding protein Vwb, is a coagulase and a virulence
                     factor. It induces clotting, not by being an enzyme, but
                     by activating host prothrombin to generate fibrin; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HDG6474554.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSGKSQVNAGSKN
                     GTLIDSRYLNSALYYLEDYIIYAIGLTNKYEYGDNIYKEAKDRLLEKVLREDQYLLER
                     KKSQYEDYKQWYANYKKENPRTDLKMANFHKYNLEELSMKEYNELQDALKRALDDFHR
                     EVKDIKDKNSDLKTFNAAEEDKATKEVYDLVSEIDTLVVSYYGDKDYGEHAKELRAKL
                     DLILGDTDNPHKITNERIKKEMIDDLNSIIDDFFMETKQNRP"
BASE COUNT          375 a           92 c          166 g          273 t
ORIGIN      
        1 attaactata acaaaaagat agttaatgct ttgtttattc tagttaatat atagttaatg
       61 tcttttaata ttttgtttct ttaatgtaga ttgggcaatt acattttgga ggaattaaaa
      121 aattatgaaa aagcaaataa tttcgctagg cgcattagca gttgcatcta gcttatttac
      181 atgggataac aaagcagatg cgatagtaac aaaggattat agtgggaaat cacaagttaa
      241 tgctgggagt aaaaatggga cattaataga tagcagatat ttaaattcag ctctatatta
      301 tttggaagac tatataattt atgctatagg attaactaat aaatatgaat atggagataa
      361 tatttataaa gaagctaaag ataggttgtt ggaaaaggta ttaagggaag atcaatatct
      421 tttggagaga aagaaatctc aatatgaaga ttataaacaa tggtatgcaa attataaaaa
      481 agaaaatcct cgtacagatt taaaaatggc taattttcat aaatataatt tagaagaact
      541 ttcgatgaaa gaatacaatg aactacagga tgcattaaag agagcactgg atgattttca
      601 cagagaagtt aaagatatta aggataagaa ttcagacttg aaaactttta atgcagcaga
      661 agaagataaa gcaactaagg aagtatacga tctcgtatct gaaattgata cattagttgt
      721 atcatattat ggtgataagg attatgggga gcacgcgaaa gagttacgag caaaactgga
      781 cttaatcctt ggagatacag acaatccaca taaaattaca aatgaacgta ttaaaaaaga
      841 aatgattgat gacttaaatt caattattga tgatttcttt atggaaacta aacaaaatag
      901 accgaa
//
LOCUS       DAMQYT010000374          868 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.382, whole genome shotgun sequence.
ACCESSION   DAMQYT010000374 DAMQYT010000000
VERSION     DAMQYT010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.382"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            92..619
                     /gene="isaB"
                     /locus_tag="PGA33_002829"
     CDS             92..619
                     /gene="isaB"
                     /locus_tag="PGA33_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HDG6474555.1"
                     /translation="MNKTSKVCVAATLALGTLIGVTVVENSAPTSKQAQAAITPYYTY
                     NGYIGNNANFILDKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGNEA
                     SQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKTLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
BASE COUNT          350 a           92 c          147 g          279 t
ORIGIN      
        1 ttataaatat aatcattagt atttttatgg ctgaaaaagt tataataaaa gtgtaaggaa
       61 taaaatattt gtatggaaaa gagagataat tatgaataaa accagtaaag tttgtgtagc
      121 agcaacatta gcattgggca cactgattgg agtaacagtt gttgaaaatt cggcgccaac
      181 aagtaaacag gcacaggcag caataacccc atattatact tataatggtt atattggtaa
      241 taatgctaat tttattctgg ataagaattt tattaacgcg attaagtatg ataatgtgaa
      301 attcaatggt attaaattag ctaaaacgaa tacgataaaa aaagtagaga aatatgatca
      361 aacttttaaa ggtgtttctg caaaaggaaa cgaagcaagt caattgcaat ttgtagttaa
      421 aaataatatt tcattaaaag atatccaaaa agcttatggc aaggacttga aaaaagaaaa
      481 tggtaaaaca aaggaagctg atagcggtat tttttactat caaaatgcta aaaagacatt
      541 aggcatctgg tttgtcgttg atcataatag agttgtcgaa gtaacagttg gacatacacc
      601 atacaaaaca agtaaataaa ataatggcat attaaggcta gagtgtgagg agtgataccg
      661 cattctagtc ttttttatta aataataacg attattgcgt cttacatagt tgtttgaaat
      721 tgataaaaat gtttatttga ttatgattat ttaaatagtt atctcacata tttcaagcat
      781 tgattaagta tatagagcac tattttgtat ttgttaatat tttcacaaaa ataaagcctt
      841 gataaatttt aaaatataat taagctca
//
LOCUS       DAMQYT010000375          865 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.383, whole genome shotgun sequence.
ACCESSION   DAMQYT010000375 DAMQYT010000000
VERSION     DAMQYT010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.383"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..172
                     /locus_tag="PGA33_002830"
     CDS             <1..172
                     /locus_tag="PGA33_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDG6474556.1"
                     /translation="DIKYLFYELLSKEKILINPGYIYGSKEKSIRLSFAFESNENIKH
                     ALYKIYTYVKKV"
     gene            362..835
                     /locus_tag="PGA33_002831"
     CDS             362..835
                     /locus_tag="PGA33_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDG6474557.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYPKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
BASE COUNT          313 a          113 c          136 g          303 t
ORIGIN      
        1 agatattaaa tatttatttt acgaattgtt aagtaaagaa aaaatattaa tcaatccggg
       61 ttacatttat ggcagtaaag aaaagagtat aaggctatct tttgcctttg aatcaaatga
      121 aaatattaag catgcgctct ataaaattta tacatatgtg aaaaaggttt aattaaaaca
      181 ataattcgaa tcattatgtg gcatgttaaa cagctaaata taagctatgc acatttaaca
      241 agcggatact cctgacgcaa ttatagtatc attacatatt gtttaatcgt ttggggtaat
      301 gtaattaaaa ggtaaaatag gcagagtgtt atacataaaa tgtaaagaaa gaggcgatag
      361 aatgtctaaa atcaggtctt ttacaatatt aagtctactt atttacttag ctatgatgtg
      421 ctatacagta gtgacctatc caaaattacc aaccaaagtg cctattcatt ataatttagc
      481 aggggatgct gataattttg ctgataaatg ggtgctgctt ttgattaata gcgcatttat
      541 agtgatttgg cttatatttt tcattgcagg tagatactat gaacgatttg ccaaatggtc
      601 acattataat catacaccac gtgaaattcg agcgattaaa ttatttttaa gtacgttaaa
      661 tttagagatt atgagctata tgtctatctt cacagtatta gaaatttggc aaatacaaca
      721 ccatcatcaa ttcaatttac tatggtttaa tatgatattt atcattatca ttggtttgac
      781 gcttgtcata ttttgtttac ttcctacaat tcataaaatg agagattctc aataaaaata
      841 taaactctca tgatgcagtt ttgaa
//
LOCUS       DAMQYT010000376          863 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.384, whole genome shotgun sequence.
ACCESSION   DAMQYT010000376 DAMQYT010000000
VERSION     DAMQYT010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.384"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(108..467)
                     /locus_tag="PGA33_002832"
     CDS             complement(108..467)
                     /locus_tag="PGA33_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HDG6474558.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(480..716)
                     /locus_tag="PGA33_002833"
     CDS             complement(480..716)
                     /locus_tag="PGA33_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HDG6474559.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
BASE COUNT          271 a          180 c          110 g          302 t
ORIGIN      
        1 acaaaatatc tttacgcaaa aataggacat aatcaaatat gtccttaatc gttattgata
       61 tcaatttgta tcccaaaaat tgggttaacc attaaaaagt tattttatta gcaattttca
      121 ggattacctg caacttttgc agttctaaat gaactaccac agccacatga agcaattgca
      181 ttaggattgt cgatttggaa accgccaccc attaatgatt gcttaaaatc aatagtcgta
      241 ccatttaata cgggtgcatc ttttttgtct actaatactt ttaatccaaa gtattctaag
      301 acttcatcat tttcaccagg cgcttcttct gcacccatac cgtatgttaa accagtgcac
      361 ccgccaccat tcactttaat ttttaaatag ccatctggca tttcatttgc tttaagcata
      421 tcttttactt cgtaagcagc tgcttctgtt aatataactg ttggcatgat aactcctcct
      481 taaaaaatcc aagtttcttt tatatgtgca tatatatttt gtaataattc ttccggcgaa
      541 tcaccttcaa caatatcacc atttactaaa gcatacaacc cggctgaaca tataccacaa
      601 tgtgtcaggc aaccatactc taacacatcg acatctgggt cattttccag ttgattaaaa
      661 acataatctc caccttttgc catgttagag agacaaaatt ctacgatcgg attcatactt
      721 caccttctta tttcatttgt tacaatatta tagcatttta aaactggtat tttaacatga
      781 tgtgctcaat tagcaacaac tgatgtttct tatcccagtt atgtaatagt gccttagtta
      841 gtacaaatat ttatttgtag tca
//
LOCUS       DAMQYT010000377          863 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.385, whole genome shotgun sequence.
ACCESSION   DAMQYT010000377 DAMQYT010000000
VERSION     DAMQYT010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.385"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(183..782)
                     /locus_tag="PGA33_002834"
     CDS             complement(183..782)
                     /locus_tag="PGA33_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDG6474560.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
BASE COUNT          259 a          155 c          124 g          325 t
ORIGIN      
        1 ctcttgttaa ttgtattttg aacgatttca tcatttgatt tttcttttag tctttttaac
       61 aaaactacct acctctattc aaagtctttc actttaaatc atatatgaat ttagaaatta
      121 tttctatctt tttgacaaaa aaataacggt ctcatttaag agaccgaaca agtaatcata
      181 ctttattttg ttgcattata taattcgtca actttttccc agttaactac attccaaaat
      241 gcgccaatgt agtcagggcg tttgttttga tattttaggt aataagcgtg ttcccatacg
      301 tctaaaccta aaataggtgt tttaccctca gttaatggat tatcttggtt tggtgtagtc
      361 acaatttcta actggccatt gtttacgact aaccaagccc aacctgaacc aaagcgtgca
      421 gctgctttgt cagcaaattc ttttttaaat tcttctaaag aaccccattg ttctttaatt
      481 ttttctacta cagtaccttt ttcttctgag tttggtgaaa gtaactccca gaataatgaa
      541 tggtttaaat gtccaccgcc attattacgt acagcagttt ggatgttagc tggtacactg
      601 tctaaattag caacaatttc ttcaatagat ttagattcta aatctgtacc ttctactgca
      661 gcatttaatt tcgtaacata cgtgttatga tgtctgtcat ggtgaatttc catagtttct
      721 ttgtcaaaat gtggttctaa tgcatcaaat gcgtatggta attttggtaa ttcaaaagcc
      781 ataaataatc atcctcctaa aatgtctgta agtaaataat aacaagcagt gactggttca
      841 acaaagaatt tgcttaaatt cta
//
LOCUS       DAMQYT010000378          851 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.386, whole genome shotgun sequence.
ACCESSION   DAMQYT010000378 DAMQYT010000000
VERSION     DAMQYT010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.386"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..82
                     /locus_tag="PGA33_002835"
     CDS             <1..82
                     /locus_tag="PGA33_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474561.1"
                     /translation="LININLSTITKNDKRFNATDDRINII"
     gene            98..550
                     /locus_tag="PGA33_002836"
                     /pseudo
     CDS             98..550
                     /locus_tag="PGA33_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            complement(644..>851)
                     /locus_tag="PGA33_002837"
     CDS             complement(644..>851)
                     /locus_tag="PGA33_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sporadically distributed, TIGR04141 family
                     protein"
                     /protein_id="HDG6474562.1"
                     /translation="VWILPISSIDNMPIKDMVHTSNVINKNFQLTNLGSVLLKNKLVE
                     WSLYLKDQRINPIIYMETPTEDRN"
BASE COUNT          316 a          119 c          144 g          272 t
ORIGIN      
        1 gcttataaat ataaatttat caactataac taaaaatgat aaaagattta acgccactga
       61 tgaccgaatt aatattattt aagtttttat caaacgcatg agtaaagtag atgcacattg
      121 ggaattaatc gaagctatta aaaatttacg tgacgaaatt gcaccaaaca cattgttaac
      181 aattaacgat gatattcccg atagaaaaac aggacttgaa cttgcagaaa aatatggtat
      241 tgatggcatc atgattggta gaggcatttt ccacaatcca ttcacttttg aaaaagaacc
      301 acgcgaacac acaagcaaag aactattaga tttgttgaga ttgcatctat cattgtttaa
      361 caaatatgag aaagatgaaa tacgacaatt caagagtttg cgtagattct ttaaaatcta
      421 tgtgcgtggc ataagaggcg ctagcgaact tcgccatcaa ttgatgaaca cacaatcaat
      481 tgcagaagta cgagcactac tagatgaatt tgaagcccaa atggacgaag acgttaaaat
      541 tgaattatag tatgagtaat aagggaaagg aggaatttaa gtttcctcct ttgtttgtgt
      601 gaaagtcttt taggtatgaa ctagggtgat ttaaaataaa agtttaattt ctatcttcag
      661 ttggcgtttc catataaata attggattaa ttctttgatc ttttaaatag agagaccact
      721 ctactaattt atttttaagt aatactgaac ctaaattagt taattgaaag tttttattta
      781 ttacgttact agtatgaacc atatctttta tgggcatatt atcaatagat gatatcggta
      841 aaatccatac t
//
LOCUS       DAMQYT010000379          845 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.387, whole genome shotgun sequence.
ACCESSION   DAMQYT010000379 DAMQYT010000000
VERSION     DAMQYT010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.387"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(142..249)
                     /locus_tag="PGA33_002838"
     CDS             complement(142..249)
                     /locus_tag="PGA33_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474563.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKHSLI"
     gene            complement(286..609)
                     /gene="isdG"
                     /locus_tag="PGA33_002839"
     CDS             complement(286..609)
                     /gene="isdG"
                     /locus_tag="PGA33_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HDG6474564.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(628..>845)
                     /locus_tag="PGA33_002840"
     CDS             complement(628..>845)
                     /locus_tag="PGA33_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HDG6474565.1"
                     /translation="KTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDRI
                     MTLSTCEDAYSETTKRIVVVAKIIKVS"
BASE COUNT          261 a          153 c           85 g          346 t
ORIGIN      
        1 actcataata tttcaaattg tataaatttc ttttatcgat actactacta taaatcatac
       61 gccccaaaat atcattatta attcttttct tcttcaaaat aaatcaaaat gatataattg
      121 atgattattt tcaaagcaca ttcaaatcaa actatgtttt agcaatttgt tgttagcatg
      181 tttgtgttca ttaaaaaaac gaccatcatc ggtatcatgt atggtcgtta caaaagctaa
      241 caataccaat tgtcataaca agtactgcaa cctctttaaa ttcaattatt tcatgtaact
      301 atagcctata tcatatgtaa ttactttgtt atttataatc gggctacttt catcttcatt
      361 tttacttcta acatgtttat gcgctgcttt aaagacatca gattttaacc aatccgtaaa
      421 agcttgcttt gatttccaaa ctgttaaaat tttcacttca tcaaaatctt cttgttctaa
      481 agtttgtgta acaaacatgc catcaaagcc ttctaatgtt tcaatcccat gtctcgtgta
      541 aaatcgttct ataatatctt ttgctgttcc ttttgttaac gtcagcctat tttctgccat
      601 aaatttcata attatcctct tttctgttta acttacctta attatttttg cgacaacaac
      661 aattcttttc gtcgtttcac tatatgcatc ttcgcacgtt gataaagtca ttattctatc
      721 ttttaccgtt acattaacat ctgaattaat tacagattta cgttttgtct catctaaaaa
      781 ttgttgataa tcttgatcat tttcaaaatc tgtacgtatg taattatctt tagtagtagt
      841 tttat
//
LOCUS       DAMQYT010000380          839 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.388, whole genome shotgun sequence.
ACCESSION   DAMQYT010000380 DAMQYT010000000
VERSION     DAMQYT010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.388"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            54..299
                     /locus_tag="PGA33_002841"
     CDS             54..299
                     /locus_tag="PGA33_002841"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474566.1"
                     /translation="MYYLKLTRWKNDGLAGFTAGSISAILVYWTNQKNEFGIKDKNDW
                     IGHKLDVGIDAVEKSAEKTVDGVENVMVKLQKVFLII"
     gene            323..>839
                     /locus_tag="PGA33_002842"
     CDS             323..>839
                     /locus_tag="PGA33_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDG6474567.1"
                     /translation="MLLCESKIINKNPKYRIIKYNDEYLMVDIISTWISLFFPFINWF
                     IPKEYVKISREEFENLNIVKPAKKNVFWPVAGSSALLGVALRKYTHLLDIQLDKKLVI
                     AICCITFIGILIFYVRLIKKSSLNIYNTKNKRSKIFLIPTLKNVCFTLFGYILFGGLT
                     MLFLDALLSMSY"
BASE COUNT          313 a          104 c          135 g          287 t
ORIGIN      
        1 gactgcaggt ttctttctgt taaacatgtc atcgattatt gacaaaaaaa tttatgtatt
       61 atctaaaact aacacggtgg aaaaatgatg gtttagctgg atttactgca ggttctattt
      121 cggcaatact tgtatattgg accaatcaaa aaaatgaatt tggaataaaa gataaaaacg
      181 attggatagg acataaacta gacgttggta tagatgctgt agaaaaatct gcagaaaaaa
      241 cagtagatgg tgttgaaaat gtcatggtga agcttcaaaa agtatttcta atcatataag
      301 ccctaagaaa tggagctggt aaatgttgct atgcgaatct aaaatcatca ataaaaaccc
      361 aaaatataga attattaaat ataatgatga atacttaatg gtcgatataa taagcacttg
      421 gattagttta ttttttcctt ttattaattg gttcatccca aaagaatacg tcaaaattag
      481 tagagaagag tttgaaaact taaatattgt taaacctgct aaaaagaatg ttttttggcc
      541 agttgcaggt agctctgctt tgctgggagt tgcattaaga aaatatacac atttacttga
      601 cattcaactt gataaaaaat tagttattgc catatgttgc atcacattta tagggatttt
      661 aatattttat gtacgcctaa ttaaaaaatc atctttaaat atttataata ctaaaaataa
      721 aaggtcaaaa atttttttaa tacctacact aaaaaatgtt tgtttcacat tatttggata
      781 tattttattt ggcggattga ctatgctatt cctagatgca ctattatcaa tgagttatc
//
LOCUS       DAMQYT010000381          837 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.389, whole genome shotgun sequence.
ACCESSION   DAMQYT010000381 DAMQYT010000000
VERSION     DAMQYT010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.389"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            310..687
                     /locus_tag="PGA33_002843"
     CDS             310..687
                     /locus_tag="PGA33_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDG6474568.1"
                     /translation="MNKKYLMIVIIILILILGGIFLKMKYDEKKYYDEQKERITIYMK
                     YNVKGYKNISFANFKENPMDGYSISGYINNDKKLSFTAGIRSVDDFQFDTDISYTDEL
                     GRKFNKNPKSVSEIKKEQNTSNK"
BASE COUNT          345 a           84 c          124 g          284 t
ORIGIN      
        1 ttgaaagtga ttaagtattt aaaacacgcc aatcttaaac cgctattgac aaatatgaag
       61 cggaaccacc gttgtttttc ttttcaggtc caattgaata aatgttatga atagaatgga
      121 taaaaatata attatttaag tgttaaagta aaccaaatca tgatttaaga ttataagaaa
      181 gaaaaattga ctaatgttaa aaccaacaat catatgggtt gtaacgttta tacaatcatc
      241 gtaaaactca atatgcttaa tttattcaaa atgttcgtca ataagtacaa tgaaaaggag
      301 attgattttt tgaataaaaa atatcttatg attgtaatta taattttaat attgattcta
      361 ggcggaatct ttttgaagat gaaatatgac gaaaagaaat attatgatga acaaaaagaa
      421 agaataacga tttatatgaa gtacaatgtg aaaggttata aaaatataag cttcgctaat
      481 tttaaagaaa acccaatgga tggttattct attagtggtt atataaataa tgataaaaag
      541 ttatcattta cagctggtat aagatctgtt gatgattttc aatttgatac cgatatttct
      601 tatacagatg aattgggtag aaaatttaat aaaaatccta agtcagtttc tgaaataaaa
      661 aaagagcaaa atacgtccaa taaataattg ttcatattgt gatgaaacaa aaatataagt
      721 cattagatga gtttaactat gttataaata tttgtagtat ctataaaaat ctcgacacta
      781 ttaaaatgat aaagtgccga ggttttctta cttatttagt taattcaaag tttatgc
//
LOCUS       DAMQYT010000382          832 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.390, whole genome shotgun sequence.
ACCESSION   DAMQYT010000382 DAMQYT010000000
VERSION     DAMQYT010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.390"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            193..>832
                     /locus_tag="PGA33_002844"
     CDS             193..>832
                     /locus_tag="PGA33_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HDG6474569.1"
                     /translation="MGEFEVKYLTGFILILLLEGIFTNSASAIEYSDLHHKSKFDSKR
                     LSNAKMSFINPTQLENKNTNDRLLKHDLLFHDMFVNDDWKKDFKVEFENEALSKKFIN
                     KDIDIFAGNYGYGCHGGATNKTQCSYGGVTLSDNNKYDDYKNIPCNLWIDGHQTEIEL
                     TAVKTKKKIVTIQELEVQLRNYLNEKYKLYEQGGDIVKGYVKYYNDDEQNVEY"
BASE COUNT          324 a           90 c          154 g          264 t
ORIGIN      
        1 tggaattttt aggtatacac tgtattggtt gatataactc aactaataat tgcgaacaga
       61 gtatttcaaa ttgaaaagta ttatgagcgt gatacataat caaaattgta ggctcaagaa
      121 ccactacata ataaaccata agcggttctt tatcatttat gtctcgctct caaatgtaaa
      181 ttaataattg ttttggggga gtttgaagtt aaatatttaa caggatttat tttaatatta
      241 ttgttagaag gaatttttac aaattcagcg agtgcaatcg aatattcaga cttacatcat
      301 aaaagtaagt ttgattcaaa gcgtctaagt aatgctaaga tgtcattcat caatccaact
      361 cagcttgaaa ataaaaacac aaacgataga ctgttgaagc atgatttgtt atttcatgac
      421 atgttcgtaa atgatgattg gaaaaaggat tttaaagttg aatttgaaaa tgaggcactt
      481 tcaaagaaat ttataaataa ggatatcgat atatttgctg gaaattatgg atacggatgt
      541 catgggggag caaccaataa aacgcaatgt agttatggtg gtgttacttt aagtgacaat
      601 aataaatacg atgattataa gaatatacct tgtaatttat ggattgacgg acatcaaaca
      661 gaaatagaac taactgcagt aaaaacgaaa aagaaaattg ttactattca agaattagag
      721 gttcaattaa gaaattattt gaatgagaag tataagttgt acgaacaagg tggcgacatt
      781 gttaaagggt atgttaaata ttataatgat gatgaacaaa atgtagaata tg
//
LOCUS       DAMQYT010000383          827 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.391, whole genome shotgun sequence.
ACCESSION   DAMQYT010000383 DAMQYT010000000
VERSION     DAMQYT010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.391"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
BASE COUNT          353 a          105 c           92 g          277 t
ORIGIN      
        1 ataataataa ttttttaaaa gaacaattaa ctaaatatca aaaatgtatt agctatctat
       61 taactaaaaa atagaataat tttttaacat agttttgttg ttttgaatta taaaaactaa
      121 agtaaaaatg ttggatgtga aagattgttg atgagaattt tgagatgtag ataaatatca
      181 aactagtcta taggtaaaaa tttataatta atttgaaaag aatgtctcaa ataatgtttg
      241 aattagcagg tgtcatacta agtaatgtta agtaaacatt ttatacttta tcagaacaaa
      301 aaagacatgc atcaacatgt cagtgaaact aatccatatt atcttaacaa cgaaattgaa
      361 tcgaacctga aaaacgtatt tacaaatcgc aactactcta tccaaggcaa aatcgcaaaa
      421 tctgatcccc gaaataacat atctcgaaaa aaattccata attcaatcat cttcaatatt
      481 taacaataca ttactagttc caaatatact catacataca tgtttcattg attgactgca
      541 tattgtcata aataataggg gaactaatcg ctaataatgt tgttaaacac aatacatagt
      601 aaagtgaaag attataaata catgaattgt tcaccattcc gccatatatt ttcaatcacc
      661 taatttccga ataaatatta caattttaat tcaataatta atatttaaga ccaaaataat
      721 cctaaaaagt caattttaat tgcgtgatat tgagttttaa gcatatttaa ataaatgagt
      781 caattcatgt aaaataattt aataaaacgt taataagtgg ttaatta
//
LOCUS       DAMQYT010000384          827 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.392, whole genome shotgun sequence.
ACCESSION   DAMQYT010000384 DAMQYT010000000
VERSION     DAMQYT010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.392"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(65..616)
                     /locus_tag="PGA33_002845"
     CDS             complement(65..616)
                     /locus_tag="PGA33_002845"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDG6474570.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
BASE COUNT          258 a          134 c          129 g          306 t
ORIGIN      
        1 ttgtaaaagg aaaagcttag gacatcatat ttatcctaag ctttgataat tagaaaaatg
       61 tgttttaaac ttctactgca tctgtatgtg gttgtaatgg gtgatctttg tcaatgtgat
      121 catagaacat tacaccattt aaatggtcaa tttcatgttg gaaaacaatt gctggatatc
      181 cttttagtcg taattgtata tcattacctt cgatgtcttt ggctttaatt gtaattctat
      241 tatgacggtg aactagacca gcaacattat catcgacact aaggcaacct tcaccagttg
      301 gtaaataagc ttcttgaacg ctatgactta caatttttgg gttcacaagc atatagtcat
      361 aagatttgcc actgccatca tctggtatta aaacagcaat catacgttta gaaatattaa
      421 tttgaggtgc agccaaacca acgcctgaac gtaaaccata tcgtttcgcg atttcctcat
      481 cttgactatt tactaaaaac tctctcatgg cgattaatgt ttctttttct tctttagtta
      541 atggtaattc taactcagct gctttttgac gcaaagttgg atgaccatct ctaatgatgt
      601 ctttcattgt taacatatat tgcaccttcc ttattttaat ttgttttagt tgaatgacag
      661 taaaaaggtt gttaagatac tcatacattt ttatgtgtaa atatctacaa agttaaccaa
      721 ctactgccaa tgtttatttt agatagtata tgtaaatttt cagatatgct aattgcttaa
      781 aaaatgatta aagtgttggt ttcaagcaat gatactttag aaattta
//
LOCUS       DAMQYT010000385          808 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.393, whole genome shotgun sequence.
ACCESSION   DAMQYT010000385 DAMQYT010000000
VERSION     DAMQYT010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.393"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..>808)
                     /locus_tag="PGA33_002846"
     rRNA            complement(<1..>808)
                     /locus_tag="PGA33_002846"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          170 a          242 c          173 g          223 t
ORIGIN      
        1 aaggatgtca agatttggta aggttcttcg cgttgcttcg aattaaacca catgctccac
       61 cgcttgtgcg ggtccccgtc aattcctttg agtttcaacc ttgcggtcgt actccccagg
      121 cggagtgctt aatgcgttag ctgcagcact aaggggcgga aaccccctaa cacttagcac
      181 tcatcgttta cggcgtggac taccagggta tctaatcctg tttgatcccc acgctttcgc
      241 acatcagcgt cagttacaga ccagaaagtc gccttcgcca ctggtgttcc tccatatctc
      301 tgcgcatttc accgctacac atggaattcc actttcctct tctgcactca agttttccag
      361 tttccaatga ccctccacgg ttgagccgtg ggctttcaca tcagacttaa aaaaccgcct
      421 acgcgcgctt tacgcccaat aattccggat aacgcttgcc acctacgtat taccgcggct
      481 gctggcacgt agttagccgt ggctttctga ttaggtaccg tcaagatgtg cacagttact
      541 tacacatatg ttcttcccta ataacagagt tttacgatcc gaagaccttc atcactcacg
      601 cggcgttgct ccgtcaggct ttcgcccatt gcggaagatt ccctactgct gcctcccgta
      661 ggagtctgga ccgtgtctca gttccagtgt ggccgatcac cctctcaggt cggctatgca
      721 tcgttgcctt ggtaagccgt taccttacca actagctaat gcagcgcgga tccatctata
      781 agtgacagca agaccgtctt tcactttt
//
LOCUS       DAMQYT010000386          800 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.394, whole genome shotgun sequence.
ACCESSION   DAMQYT010000386 DAMQYT010000000
VERSION     DAMQYT010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.394"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..>800
                     /locus_tag="PGA33_002847"
     CDS             <1..>800
                     /locus_tag="PGA33_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDG6474571.1"
                     /translation="LISGKTGSGKTMIFDAMTYALFGKASTEQREENDLRSHFADGKQ
                     PMSVTFEFQLNHRIYKVHRQGPYIKEGNTTKTNAKFDVFEMVDGKYEIRESKVISGTQ
                     FIIELLGVNADQFRQLFILPQGEFKRFLISNSREKQGILRTLFDSEKFEAIREILKEE
                     VKKEKAQIENRYQQIDLLWQEIESFDDDNIKGLLEVATQQIDKLIENIPLLQARSKEI
                     LASVNESKETAIKEFEIIEKKTLENNILKDNINQLNKNKIDFVQLKEQ"
BASE COUNT          343 a           96 c          140 g          221 t
ORIGIN      
        1 tttaataagt ggtaagactg gatcgggtaa aacaatgatt tttgatgcaa tgacttatgc
       61 cttgtttggt aaagcatcaa ctgaacaaag agaagaaaat gatttgagaa gtcatttcgc
      121 tgatggtaaa cagccgatgt cagtaacatt tgaatttcaa ttaaatcatc gaatttataa
      181 agtgcataga caaggccctt atatcaaaga aggtaataca acaaaaacga acgctaaatt
      241 tgatgtattt gagatggtgg atggcaagta tgaaattaga gaaagtaaag taatttcagg
      301 tacccaattc attattgaat tattaggagt aaatgcagat caattccgac aattgtttat
      361 tttgcctcaa ggtgaattca aacgcttttt aatatcaaac agtcgtgaaa agcaagggat
      421 attaagaaca ctgtttgaca gtgaaaaatt tgaagctata cgagaaatat taaaagaaga
      481 agtaaaaaaa gaaaaagctc aaatcgagaa tagatatcaa caaattgacc ttttatggca
      541 agaaattgaa tcatttgatg atgacaatat aaaaggctta ttagaggttg ccactcaaca
      601 gatagacaaa ttgattgaaa atataccact tttacaagct aggtcgaaag aaatactagc
      661 atctgtaaat gaaagtaaag aaactgctat taaagaattt gaaataatag aaaagaaaac
      721 attagaaaat aatatattaa aagataatat taatcaactc aacaaaaata aaattgattt
      781 cgttcaattg aaagaacaac
//
LOCUS       DAMQYT010000387          799 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.395, whole genome shotgun sequence.
ACCESSION   DAMQYT010000387 DAMQYT010000000
VERSION     DAMQYT010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.395"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            334..>799
                     /locus_tag="PGA33_002848"
     CDS             334..>799
                     /locus_tag="PGA33_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HDG6474572.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKASTLEVRSQATQDLS
                     EYYNRPFFEYTNQSGYKEEGKVTFTPNYQLIDVTLTGNEKQNFGEDISNVDIFVVREN
                     SDRSGNTASIGGITKTNGSNYIDKVKDVNLIITKNIDSVTSTSTSSTYTINKE"
BASE COUNT          327 a           82 c          142 g          248 t
ORIGIN      
        1 ataaaagttg taaaagtaaa gtgaattaaa aacgaacatt aaatttaggc actgtgaaag
       61 cgcagtgtct tttttgtgtc gaaattgtgt acagaataag tagttaaata aagattaagt
      121 tgagataaag tgttattcgt aaataaaaga gagtagatcg ataggaattg aatgatatta
      181 gttaactatt tattaaatta cttaataatg attaattttt agttaaagta agtttaatgt
      241 gaagcacgac cattgctcat tataatgaat gaggattgtt cgtattgcgt aatagaataa
      301 atcaaataga ctaaaaattg ggagcataga attatgaaat taaaaaatat tgctaaagca
      361 agtttagcac tagggatttt aacaacaggg atgattacaa ctactgctca gccagtaaaa
      421 gcaagtacat tagaggttag atcacaagct actcaagact tgagtgaata ttataataga
      481 ccgttctttg agtatacaaa tcagtcagga tataaagagg aaggaaaagt gacgtttact
      541 cctaattatc aacttataga tgtaacttta actgggaatg aaaagcaaaa ttttggtgaa
      601 gatatttcta atgtagatat atttgttgta agagaaaatt ctgatagatc tggtaataca
      661 gcttcaattg gtggtattac taaaacaaac ggttcaaatt atattgataa agtaaaagat
      721 gtaaatttaa taattactaa aaacatcgat agtgttacat caacgtcaac atcatctaca
      781 tatacaatta ataaagaag
//
LOCUS       DAMQYT010000388          793 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.396, whole genome shotgun sequence.
ACCESSION   DAMQYT010000388 DAMQYT010000000
VERSION     DAMQYT010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.396"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            350..793
                     /locus_tag="PGA33_002849"
     CDS             350..793
                     /locus_tag="PGA33_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474573.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPV
                     TFANAYLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMFASIYFIKIVTSYFTKKLLEIKFNSK"
BASE COUNT          287 a           92 c          112 g          302 t
ORIGIN      
        1 atttcctcgt gtaaccgata aatcatcgta aaaaaagtct ttgaattttt gcatgctaaa
       61 tcctttcttt tcatttttta aaattatttt gaatgcaata tgattaaggc tagattcata
      121 atatatgaaa catatttacg attaaaattt atcattgtac ctacctaaaa agcattagtt
      181 tgaaaaatta gcagactgta ttttagcgtc aaatcaccat aaatgaaatt gtaatatgga
      241 tttttgtaga tttgatacat atatttctgt aaaatatata aaatacactt tcaattgacg
      301 ctaatgttta aaattacgtc aatattataa aggagagaaa tccttaatta tgaataaagc
      361 atcatttgat aaaaaagtta aaaagcaatt atggtttttg aacaagaagg agaaacaagc
      421 attagatcaa cgattgtctt ctatatctga tgatgacagc gttaacttaa ataagcctgt
      481 tacctttgct aatgcttact taagacaaaa tgtatttcga aataaagaaa caaaaagtta
      541 tagtatgttt gttacattag tagtgatgat gtttgcttat gttgctttat taggtttgtt
      601 tttatttggg ctaattacaa gtctatctgg cgtacaattt ttcgttagtc ctaaggtaga
      661 tttatctaca actgttgtta ttttaacaat tattggagcg attttattaa tgtttgctag
      721 catatatttt ataaaaattg tgacatcata ctttactaag aaacttttag aaattaaatt
      781 taattcgaaa tag
//
LOCUS       DAMQYT010000389          793 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.397, whole genome shotgun sequence.
ACCESSION   DAMQYT010000389 DAMQYT010000000
VERSION     DAMQYT010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.397"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            32..172
                     /locus_tag="PGA33_002850"
     CDS             32..172
                     /locus_tag="PGA33_002850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474574.1"
                     /translation="MAKKNDYRFTYKDYKFLKQYGVSNTFICKMYHISIEDLEFFEVM
                     NR"
     gene            323..643
                     /locus_tag="PGA33_002851"
     CDS             323..643
                     /locus_tag="PGA33_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HDG6474575.1"
                     /translation="MKPVLDIKKTLGQLNFLGVDEKYKYENGERTDKTVYAYKLASQE
                     QGEQITVKTPNKVELDYLQECELVEPDVKMYVQMSGDFGTIAYSWNAEDIKVVGSNSA
                     LKQK"
     gene            657..>793
                     /locus_tag="PGA33_002852"
     CDS             657..>793
                     /locus_tag="PGA33_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474576.1"
                     /translation="MDYYTADRLYRYTNSSNLSEPILNYVASRINWGDKVSLMTLAKE
                     I"
BASE COUNT          300 a           85 c          150 g          258 t
ORIGIN      
        1 tgcatatttt cgtatattag aagtttgtga aatggcaaaa aagaatgatt atcgatttac
       61 ttataaggat tataaatttt taaaacaata tggtgtgtct aatacattta tttgtaagat
      121 gtatcatatt tcaattgaag acttagaatt ttttgaggtg atgaaccgat aggtttgatt
      181 aaaaatatga taggtcagga gaagcataga cactccatta tcaaaaatgc agtggtacgg
      241 atttgcggga gttattccta ttcgtgcgac cctttagaag tcgtttaatt ttattaaaaa
      301 ttttgtaaag gatggatata ttatgaaacc agtattggat attaaaaaaa ctttaggaca
      361 attaaacttt ttaggtgtag atgaaaaata taaatatgaa aatggagagc gtactgataa
      421 aactgtttat gcatataaat tagcgagtca agaacaaggc gaacaaatca ctgttaaaac
      481 gcctaataaa gtggaattag actatttaca agaatgcgaa ttagttgaac cagacgttaa
      541 aatgtatgtt caaatgtcag gcgattttgg aactatcgca tatagctgga atgcagaaga
      601 tattaaagta gttggttcta atagtgcact taaacaaaaa taattaggtg atgtaaatgg
      661 attattatac agcagataga ttatatcgct acacaaatag ttcaaatttg agtgaaccta
      721 ttttaaacta tgttgctagt cgtataaatt ggggcgataa agtttcatta atgactttag
      781 ctaaagaaat aca
//
LOCUS       DAMQYT010000390          781 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.398, whole genome shotgun sequence.
ACCESSION   DAMQYT010000390 DAMQYT010000000
VERSION     DAMQYT010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.398"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..267
                     /locus_tag="PGA33_002853"
     CDS             <1..267
                     /locus_tag="PGA33_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HDG6474577.1"
                     /translation="PAITKSIKKLLAKDLVVESHNEFNKREVNYSLTQKGKKLSYIHD
                     ELHEKSVKKYEEVLKVFDDDEMAVIIEFLNRSIEELKKEDGLND"
     gene            260..>781
                     /locus_tag="PGA33_002854"
     CDS             260..>781
                     /locus_tag="PGA33_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDG6474578.1"
                     /translation="MINSNIRLYILGFLAFFASLIQNIYTPIIPRLYDDFQVPLFWIN
                     ATVGGFIFIVAVMQIVLGRSIDSRDSKKVLLTGLGIVIISSFICAVTHNFILFAISRL
                     FQAIGCGIIPLVTLTLLAKLSTDNGRAQAMANYQIFLSCAPALAPILGSTLGARWDYI
                     GIFSFLLVISIVLF"
BASE COUNT          274 a           99 c          135 g          273 t
ORIGIN      
        1 ccagcaatta ctaaatcgat aaaaaaactt ttagcgaaag acttagtagt tgaatcgcac
       61 aatgaattta acaaaagaga agttaattat tcattaacac aaaaaggaaa aaaattatct
      121 tatatacatg atgaattaca tgaaaaatcg gtaaaaaaat atgaagaggt acttaaagtc
      181 tttgatgatg atgaaatggc agttattata gagtttttaa accgtagtat agaagaatta
      241 aaaaaagaag atggtttaaa tgattaatag taatatcaga ttatatattc taggattttt
      301 agcatttttc gcatcattga ttcaaaacat atacacgcct attattccga gactatatga
      361 cgattttcaa gtaccactat tttggataaa tgcgacagtt ggcggtttta tatttatcgt
      421 tgcagttatg caaattgtac ttggtagaag tatagattct cgtgattcga aaaaagtact
      481 tttaacggga ttgggaatag taatcatatc tagctttatt tgtgcagtga cacataactt
      541 tatattattt gctatatcaa ggttatttca agcaattggt tgcggaatta ttcctctcgt
      601 gactttaact ttattagcca aactaagtac agataatggt agagctcaag ctatggcgaa
      661 ctatcaaata tttttatcat gtgcaccagc tttggcacca atcttaggga gtactttagg
      721 tgcaagatgg gattatattg gtatttttag ttttttgctc gttatatcta tcgtattatt
      781 t
//
LOCUS       DAMQYT010000391          778 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.399, whole genome shotgun sequence.
ACCESSION   DAMQYT010000391 DAMQYT010000000
VERSION     DAMQYT010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.399"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            448..>778
                     /locus_tag="PGA33_002855"
     CDS             448..>778
                     /locus_tag="PGA33_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDG6474579.1"
                     /translation="MLLCDVRVIYKNPKYKVIQHNGEYLLVDLVSTWFVYFFPFINWF
                     IPKKYAIISEEEFENLNVVKPNKNNVFWSVIGSSVLFGVTLRKYIHVFDVQLDKLVVM
                     ILCALALI"
BASE COUNT          277 a           83 c          148 g          270 t
ORIGIN      
        1 atgatcgaaa ttggtagtat cgcatattta aacggtggtt caaaaaaata taatcatatc
       61 ttaaatcagg aaaatagata gtaatgtttg aatataccaa gtgaaaatgc ccattgtgat
      121 tgataaagga aaaaactgtt ttaatttaaa aatgaaatat agagcgtgtt attttgtatg
      181 atagctctta taacattggc gtttttaatc caatagtatg gacggtttta gctggttttg
      241 ttgcggaatc tgtttagaca attcttaaaa attggacttt tcatactaat aaatttggat
      301 taagagataa aactaattgg ataagacatt aaatagacgc tgtttaaaaa taccacagaa
      361 aaagctgtag atagtgttgg aacgatagtt ggtgaaggta caaaaagtat tggggatcat
      421 ttaaaactaa tgaaacggga gtggtaaatg ttgctttgcg atgtcagagt catttataaa
      481 aatccgaaat acaaagtcat tcaacataac ggtgaatact tattagtcga tttagtaagc
      541 acttggttcg tgtacttttt tcctttcatt aattggttca ttccaaaaaa gtacgcgata
      601 attagcgaag aagaatttga aaatttaaat gttgttaaac caaataaaaa taatgttttc
      661 tggtcagtta taggaagttc ggttttgttt ggagttactt taaggaaata catacatgtt
      721 tttgatgttc aattagataa gctagttgta atgatattgt gtgctctcgc tttaattt
//
LOCUS       DAMQYT010000392          761 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.400, whole genome shotgun sequence.
ACCESSION   DAMQYT010000392 DAMQYT010000000
VERSION     DAMQYT010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.400"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..192
                     /locus_tag="PGA33_002856"
     CDS             <1..192
                     /locus_tag="PGA33_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDG6474580.1"
                     /translation="NVKQLRKRYDIPTSKAPKLLLKGSGNLKGSSVGYKDIEFTFVEK
                     KEENIYFSDSLDYKKSGDV"
     gene            244..>761
                     /locus_tag="PGA33_002857"
     CDS             244..>761
                     /locus_tag="PGA33_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDG6474581.1"
                     /translation="MIKRVNKLVLGISLLFLVISITAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVNSQMAIQNKGEALKIKGMLLKIDRNTRSA
                     KGFYYTNEIKTEKYEVAQDNQKKYPVKMINNKFISTEEVKEENIKKEIENFKFFAQYS
                     NFKDLMNYKDGD"
BASE COUNT          335 a           71 c          145 g          210 t
ORIGIN      
        1 aatgtaaaac aattacgcaa aagatatgat ataccgacga gtaaagctcc aaagttattg
       61 ttaaaaggtt cagggaattt aaaaggctca tcagttggat ataaagatat tgaatttacg
      121 tttgtagaga aaaaagaaga aaatatatac tttagtgata gcttagatta taaaaaaagc
      181 ggagatgtat aatcatggct caatcagaat atgaaatcaa tcccggaaaa agagagtgat
      241 gaaatgataa aacgtgtaaa taaattagtg cttggtatta gtcttctgtt tttagtcatt
      301 agtatcactg ctggttgtgg catgggtaaa gaagcggaaa taaagaaaag ttttgaaaaa
      361 acattgagta tgtatccgat taaaaatcta gaggatttat acgataaaga aggatatcgt
      421 gatgatcaat ttgataaaaa tgataaggga acatggattg taaattctca aatggcaatt
      481 caaaataaag gagaagctct aaaaataaaa ggcatgcttt tgaagataga tagaaataca
      541 agaagtgcaa aaggatttta ctatactaat gaaataaaga cggagaaata cgaagtagct
      601 caggataatc aaaaaaaata tccagttaaa atgattaata ataaattcat ttctactgag
      661 gaagttaaag aagaaaacat aaaaaaagaa atcgaaaact ttaagttttt tgcgcaatat
      721 agcaatttta aagatttaat gaattataaa gatggagata t
//
LOCUS       DAMQYT010000393          744 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.401, whole genome shotgun sequence.
ACCESSION   DAMQYT010000393 DAMQYT010000000
VERSION     DAMQYT010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.401"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            163..411
                     /locus_tag="PGA33_002858"
                     /pseudo
     CDS             163..411
                     /locus_tag="PGA33_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
BASE COUNT          301 a           89 c          129 g          225 t
ORIGIN      
        1 ttatatcgta ataagtcttt ttctgtagta agagacttta tcgagaagca agaatttccc
       61 gaaaattata aaatagcatg gtattaaagc atcctttcta cacagataaa gagaaagcga
      121 tgctattttt atatgctttt ttcattattc gttgaaaaag aagagtcaca aaagttaaca
      181 tatttaaaag aacttggtga agatggcgaa tataaatatg ttgccaaaat agatagtaaa
      241 acatctaaat tatgtcattc actcaacgga aaaatattta aagttaaaga tatgatacca
      301 ggtgtgaatg cgccacctat gcatccttgg tgtagaagta ccacagtgcc acatgtcggc
      361 aattggcgag acaagttctt taaagagcgt gaaggtaaat atcaagtaga aggttgtttt
      421 attgaatcag gtgcacttaa taataaaagt gatgaatacg gtatcaaaag gaacagacat
      481 gctcaaattt attataattc agttagaaat cgagataaac agatagagat ttctaaaatt
      541 gctaaaaata ctaatataaa taaaaattta attcaaagag tttatgaaca tatatttgaa
      601 aataagtatt tattagaaag tggatttaaa caatttgatc ctgactttta tatggctcaa
      661 agttggcaac gtttaagaga agggaaaaat attaaaaaaa tggatataat aatgtagaaa
      721 catgaagctc ttgaacacta tcta
//
LOCUS       DAMQYT010000394          734 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.402, whole genome shotgun sequence.
ACCESSION   DAMQYT010000394 DAMQYT010000000
VERSION     DAMQYT010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.402"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            156..356
                     /locus_tag="PGA33_002859"
     CDS             156..356
                     /locus_tag="PGA33_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474582.1"
                     /translation="MTNPTRTKRCGGDVLYGIDNHFQLLLYISENHCQLETKTFFEYF
                     LRIVNKTHDYAILENKKICISH"
     gene            433..564
                     /locus_tag="PGA33_002860"
     CDS             433..564
                     /locus_tag="PGA33_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474583.1"
                     /translation="MLLPSSKSHIEMMCEMFKLRSFCENKDNNYSFYNSEKSVYDNN"
BASE COUNT          278 a           84 c          112 g          260 t
ORIGIN      
        1 gaaccttttc tgaaccatac gcatagcgta tggttttctt tttacaaaaa acaaaagcag
       61 taaacctaaa gtgtcgtaag ggtttactgc ttttataaaa cgttgttata acgtatattg
      121 tcttttacgg gcatataaaa ggggaatatt tgaaaatgac caatccaaca agaacgaaac
      181 gttgtggggg ggatgttcta tatggtattg ataatcattt tcaactacta ttatacatta
      241 gtgagaatca ttgtcaatta gaaactaaaa ctttttttga atatttttta agaatagtaa
      301 ataaaacgca tgattacgct attttagaaa ataaaaaaat ttgtatttct cattagaatt
      361 agaatattta aaagtgatga ggtttaaaca ttatattgtt tacatactcc ttttgaattc
      421 atacattatg aaatgttact tccaagttca aaatcgcaca ttgaaatgat gtgtgaaatg
      481 tttaaactac ggtcattttg tgaaaataaa gataataact attcatttta caatagtgaa
      541 aagtcagtat atgacaacaa ttaatattgc ggtaaggcct tgtgttacag tattctatat
      601 ttaagtactg caatcagaat taacagaatg ccattaactg attattaaat atttgagtta
      661 ataaataatt aatgattgta gcttgaaaaa tttaaaacat ggttattgat ttgtgataaa
      721 atttaaacgt aaac
//
LOCUS       DAMQYT010000395          728 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.403, whole genome shotgun sequence.
ACCESSION   DAMQYT010000395 DAMQYT010000000
VERSION     DAMQYT010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.403"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(214..546)
                     /locus_tag="PGA33_002861"
     CDS             complement(214..546)
                     /locus_tag="PGA33_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HDG6474584.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
BASE COUNT          240 a          127 c           79 g          282 t
ORIGIN      
        1 aacaataatg cacttaaagt tttgaactga cgaaatttac aaaatgattc tcgtctctct
       61 aattacttaa aacgggttct ataataaatc gtactgatgg aaagttttta ctttttatct
      121 gtccgatttt ttgaatttga agataaaaaa acatctaaaa cgcataagaa gataactcta
      181 cttcttcaaa attacgttat agatgctttt ttattaattc atatgcatgt cgtgaatttt
      241 catcattaaa ccgtgtggga cgtcatcatg tttaacaatc tcttgtttat aaaatgtctt
      301 cccatcatct ttagaaacta attttacaaa gtctcctttt ttcggcttga aatcattaga
      361 tggttctact ttgatattat gtttgactaa tccatctttt tcactgacaa ctttttctgc
      421 atttgtatta ctattcatat aaccaaaata agtcgttttt tgactagaaa aaatcattaa
      481 acatacaacg gcaataccaa taacaaccaa tgcaatagat aaacctagac ccattttttt
      541 acccatttat agactccttc atttttcaaa aatacctatt atcatgagaa cttaattatt
      601 actaatttta gtcacgcatt agaaacactt tagttttcaa tagttttacg ttctgtcttt
      661 ttaccccagt attgataata agtacattct atatatccat taaataactt acgtcgcttt
      721 gttgcttt
//
LOCUS       DAMQYT010000396          724 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.404, whole genome shotgun sequence.
ACCESSION   DAMQYT010000396 DAMQYT010000000
VERSION     DAMQYT010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.404"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            7..231
                     /locus_tag="PGA33_002862"
     CDS             7..231
                     /locus_tag="PGA33_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474585.1"
                     /translation="MNDKSVTYKYFPNNDENIKPGIIQMDIDSLEVINAEKSSLEKNT
                     RDNYFIHAIDRIYINTSKGLFPESELVAWG"
     gene            266..388
                     /locus_tag="PGA33_002863"
     CDS             266..388
                     /locus_tag="PGA33_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474586.1"
                     /translation="MKVIFYCPKRADDILKASMEYQSTDYKRRYISWKTMIAMK"
     gene            364..462
                     /locus_tag="PGA33_002864"
                     /pseudo
     CDS             364..462
                     /locus_tag="PGA33_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head morphogenesis protein"
BASE COUNT          288 a           87 c          113 g          236 t
ORIGIN      
        1 aaagaaatta atgataaaag tgttacatat aaatattttc ctaataatga tgaaaatatt
       61 aagcctggta ttattcaaat ggatatagat agtcttgaag ttatcaatgc tgaaaaatct
      121 agtttagaaa aaaatacaag agataattat tttattcatg ccatagacag aatatacata
      181 aatactagta aagggctatt tcctgaatct gaactcgtag catggggata aagaaagttt
      241 actttaaaaa ttttaacctt caaatgtgaa ggttattttt tattgcccaa aacgtgctga
      301 tgacatttta aaagcaagta tggaatatca gtcgacagac tataaacgga ggtatatctc
      361 atggaaaaca atgatagcaa tgaagtagct tatttgttaa aagatggtaa atttacaaaa
      421 gtatatgtca atcaagatag tgtatctttt gtgccagggt aacaagcaac cgaattatta
      481 tttaacagta aaccgaattc aattgttatg ttacataacc atcctggaca gtcaggattt
      541 tcagaatatg atttatttac attttttaag catccatcaa taaaatcaat gacaatcgtt
      601 actaataagg gacaagtaaa atttattacc aagtcaaata ggtttcatgg taaaatagtg
      661 agtaaatttt gtgctaaata ttttacgcat attaatatca ttaatgatag ccatattgaa
      721 aaat
//
LOCUS       DAMQYT010000397          724 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.405, whole genome shotgun sequence.
ACCESSION   DAMQYT010000397 DAMQYT010000000
VERSION     DAMQYT010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.405"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            101..256
                     /locus_tag="PGA33_002865"
     CDS             101..256
                     /locus_tag="PGA33_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474587.1"
                     /translation="MMVKKLLKAIKQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
     gene            264..704
                     /locus_tag="PGA33_002866"
                     /pseudo
     CDS             264..704
                     /locus_tag="PGA33_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          269 a           75 c          131 g          249 t
ORIGIN      
        1 tgttattaat ttggaaaata ctggtcaaag atgggagctc ttaaaagcgt tattgtattt
       61 tttagtcaat acaaatagat tgccgtaata ataatcgtac ttgatggtta aaaaattact
      121 taaggctata aagcaaaact ttttatatga gcagtcgaat ataacgttta aaatgattgt
      181 ttttggatat aaacgattaa gtaaaatgct ttttcagttt gaaattaatc atataaattt
      241 cttatgggag ggttgatatc ttaatgatta acattatttc agctatagga tctattggaa
      301 catttattat ggctttattt tattttgtat cagtttcagt tcaactttat caaatgaaaa
      361 ttagctttct gccagcttta ggttttaacc aaattttatt agaaagggag gaggatcaac
      421 ttaatataat gaattcggca acagaagagc atcatcataa agattatatt aaactatata
      481 atttaggtgg cggtgctgct aaaaaaattg caatagaggt tttattgggg aaggataaag
      541 tcattcagaa aaaatacgtg catattttac ctagtaaaga agggtacatg ttaccaatta
      601 ataaaaatgt gtacgaagaa ttagaaagaa cgattgagaa caatggtcat gaagctgatt
      661 tgaatgtacg tatgacttat tatcataatg taagtcgcaa acaataggaa gttatattaa
      721 aagg
//
LOCUS       DAMQYT010000398          716 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.406, whole genome shotgun sequence.
ACCESSION   DAMQYT010000398 DAMQYT010000000
VERSION     DAMQYT010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.406"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            23..>716
                     /locus_tag="PGA33_002867"
     CDS             23..>716
                     /locus_tag="PGA33_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDG6474588.1"
                     /translation="MAKSCLHILTNNEYATTRCQDGIVLFWPIDGEIELQKFRKSKII
                     EDDIYIINHLDVFSIKNNKKTIMLYLSSDWFAELGFTFFNYHYTAKLIKSSYNLKCLL
                     LKLTYRYLDNQPLNDADIRKLQDIIKIIAKEASMDKKIAQNQYRYAYYGDLRDELEYI
                     YQNVNQRLTLKSVADKLFVSKSNLSSQFHLLMGMGFKKYIDTLKIGKSIEILLTTDST
                     ISNISEHLGFSSS"
BASE COUNT          270 a           90 c          118 g          238 t
ORIGIN      
        1 aaatatagaa agaggtaaag ttatggcaaa atcatgcttg catatactta ctaataatga
       61 atatgcgaca acgcgttgcc aagatggcat agtcttattt tggccaattg acggggaaat
      121 cgaactacaa aaatttcgta aaagtaaaat aattgaagat gatatatata ttattaatca
      181 tctggatgta tttagtatta agaataataa aaaaacgatc atgttgtatt tgagtagcga
      241 ttggtttgcg gaattaggct ttactttctt taattaccac tatacagcaa agttgattaa
      301 atcatcctat aatttgaaat gtctactatt aaaattgaca tatcgatacc ttgataatca
      361 gcctcttaat gacgctgata ttagaaaatt acaggatatt attaaaatca ttgcaaaaga
      421 agcaagtatg gataaaaaga ttgcacaaaa tcaatatcga tatgcgtatt atggtgattt
      481 gcgtgatgag ctcgaatata tttatcaaaa tgtaaatcaa cgattgacat taaaaagtgt
      541 cgctgataaa ttatttgtct caaagtcaaa tttgtcatca caattccact tacttatggg
      601 catgggtttt aaaaaatata ttgatacttt gaaaattggt aaatcgattg aaattctact
      661 tactactgat agtactatta gcaacataag tgaacattta ggttttagta gtagct
//
LOCUS       DAMQYT010000399          712 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.407, whole genome shotgun sequence.
ACCESSION   DAMQYT010000399 DAMQYT010000000
VERSION     DAMQYT010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.407"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            242..598
                     /gene="sarT"
                     /locus_tag="PGA33_002868"
     CDS             242..598
                     /gene="sarT"
                     /locus_tag="PGA33_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarT"
                     /protein_id="HDG6474589.1"
                     /translation="MNDLKSKSNIKLMKRVLTTYELRKYLKKYFCLTLDNYLVLAYLD
                     VFKNDEGKYFMRDIISYIGIDQSRIVKSVKDLSKKGYLNKCRDPHDSRNVIIVVSVKQ
                     HNYIKNLLSEININET"
BASE COUNT          301 a           62 c          100 g          249 t
ORIGIN      
        1 aacctaaata ttttatatat aatatatata tataaaatat aaataactaa aaatcacaga
       61 ataacaaatg acaaattcat ttattatcca aaaataaaaa caattgaaca aaatcaaaat
      121 atagtgttat gattcatttg tgtttatagc tagcaccaca aactaaatat atttggagtt
      181 attactagtt aaagacaaat attatttgac gtgacaatta aaagtaaggg atgaagactt
      241 gatgaatgat ttgaaaagca agagcaatat taaattaatg aaaagagtat taactacata
      301 tgagctgcgt aaatatttaa aaaaatattt ttgtcttaca ttagataatt atttagtatt
      361 agcatattta gatgtattta aaaatgatga aggtaaatat tttatgagag acattataag
      421 ttatatcggt atagaccaat ctagaattgt taaaagcgta aaagatttat caaaaaaggg
      481 ttacttgaat aaatgtagag acccacatga tagtagaaat gtaatcattg ttgtttctgt
      541 aaaacaacac aattatatta aaaatctttt gagtgaaata aatattaatg aaacataaag
      601 tatgtttcga gattttaata tcttggtgca gttaaatgta ttttttaaat gcgttaaggt
      661 attttttatt ttagaaattc aatgctattg agttgatggg tttttccaac ag
//
LOCUS       DAMQYT010000400          709 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.408, whole genome shotgun sequence.
ACCESSION   DAMQYT010000400 DAMQYT010000000
VERSION     DAMQYT010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.408"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            37..579
                     /locus_tag="PGA33_002869"
     CDS             37..579
                     /locus_tag="PGA33_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDG6474590.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKERYKKIRQY"
BASE COUNT          265 a           84 c          143 g          217 t
ORIGIN      
        1 ttataatggt taaattaaca gaaggtggtg tggagtatgg tcagagaatc catacctaaa
       61 gaaggggaaa acattaaaat tcagagttat aaacatgatg gtaagattca tcgcgtttgg
      121 tctgaaacaa caatattaaa gggtactgat catgttgtca ttggtggaaa tgatcatacg
      181 ttagtgactg aaagtgacgg aagaacttgg ataacacgtg aaccggcaat tgtttacttt
      241 cactcagaat attggtttaa tgttatctgc atgtttagag aagatggaat ttattactat
      301 tgcaatttgt catcaccatt tgtttgtgat gaagaggcat taaaatatat agactatgat
      361 ttagatatta aagtatatcc aaacggaaag tatcatttat tagatgaaga tgaatatgag
      421 caacatatga atcaaatgaa ttatccgcat gatattgata ttattttaag acgcaacgtg
      481 gacattcttc aacaatggat agaacagaag aagggcccat tcgcgccaga ttttatcaaa
      541 gtttggaaag aacgttacaa aaaaattaga cagtattaag caaataaagt gtaaatactg
      601 attaaatcat ttatattgac ttagtgatgt aaagaagtat gataactttt gctgtcgtgt
      661 ggggacacag tatccataga ttaaattgaa tagaatataa catacgaat
//
LOCUS       DAMQYT010000401          704 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.409, whole genome shotgun sequence.
ACCESSION   DAMQYT010000401 DAMQYT010000000
VERSION     DAMQYT010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.409"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            61..696
                     /locus_tag="PGA33_002870"
     CDS             61..696
                     /locus_tag="PGA33_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDG6474591.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
BASE COUNT          256 a           98 c          142 g          208 t
ORIGIN      
        1 gctctaactc gtttgaatac atagatataa ttcattcaaa cacgaaggag gaaatagcga
       61 atgagtgacc aagttaaaat tcctcgagca actttaaaac gtttgccgtt atattataga
      121 tttgtcagtt cattaaaatc taaaggtata gatcgtgtaa attcaaaagc gattagcgat
      181 gcgttacaaa ttgactcggc aacaattcgt cgtgactttt catattttgg cgaattaggt
      241 aaaaaagggt acggatataa tatagatagt ttattggatt tctttaaatc tgaactaagc
      301 gagagtgaca tgatcaaaat cgcaattgtc ggagttggga acctagggaa agctttgctc
      361 acatataact tttcaataca tgacgatatg acgattacag aagcgtttga cgtaaaagaa
      421 gatgttattg gccagaaaat agggaacgtt attgttaaag ataacgatga attaataaca
      481 acattgaaga aggaagaaat agatgttgtg attctaacta caccagaaag agttgcacag
      541 aaagttgcag atgaactcgt ccaagctggt gtgaaaggta ttttaaactt cactcctggt
      601 agaattaata cgccttcaga tgtgcaagta catcaaattg acttaggtat agaattacag
      661 tcattattat tctttatgaa aaattacagt gaataaatgt gatg
//
LOCUS       DAMQYT010000402          697 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.410, whole genome shotgun sequence.
ACCESSION   DAMQYT010000402 DAMQYT010000000
VERSION     DAMQYT010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.410"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            41..>697
                     /locus_tag="PGA33_002871"
     CDS             41..>697
                     /locus_tag="PGA33_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDG6474592.1"
                     /translation="MKRLIGILLCNLFILTACSASVDKTSNSTKTTDYKIENGETLKV
                     PEKPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVERVAK
                     AKPDLIVVDAMDKNIKKYQKIAPTIPYTYNKYNHKEILKEIGKLTNNEDKAKKWIEEW
                     DDKTRKDKKEIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKD
                     K"
BASE COUNT          303 a           77 c          121 g          196 t
ORIGIN      
        1 ttgaaaatga atatcaattg aaaaacaaaa ggatgatatt atgaaaagat taattggaat
       61 tttattatgt aatttattta ttttgactgc gtgttcagca tctgtcgata aaacaagtaa
      121 ctcgacaaag actacagact ataaaattga aaatggtgaa acactgaaag taccagagaa
      181 acctaaaaga gttgctgtat taactggatt ttatgttggt gattttataa agttaggaat
      241 caaaccaatt gctgtttcag atataactaa agattcttca attttaaaac cttatttaaa
      301 aggggttgat tatattggag aaaatgatgt tgaaagagtt gctaaagcaa aaccagattt
      361 aattgttgta gatgctatgg ataaaaatat aaaaaaatac caaaaaatag cgccaacaat
      421 cccatataca tacaataaat acaatcataa agaaatatta aaagaaatag gcaagttgac
      481 taataatgaa gataaagcta aaaaatggat tgaagagtgg gacgataaaa ctagaaaaga
      541 taaaaaagaa attcaaagta aaattggtca agcaacagca tctgtgtttg aaccagatga
      601 aaagcaaata tacatatata actctacatg gggtcgtggt ttggatattg ttcatgatgc
      661 attcggtatg ccaatgacaa agcaatataa agataaa
//
LOCUS       DAMQYT010000403          696 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.411, whole genome shotgun sequence.
ACCESSION   DAMQYT010000403 DAMQYT010000000
VERSION     DAMQYT010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.411"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(228..659)
                     /locus_tag="PGA33_002872"
     CDS             complement(228..659)
                     /locus_tag="PGA33_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDG6474593.1"
                     /translation="MFKNILLPYDFENDFSAIPDYLEKVTDEDSVVVIYHVVTENDLA
                     ISVKYYNKHKEDIIREKEKKLTPFLRELEKRDIQYKIDVDFGHIKDTILEKITSGDIN
                     NGEFDLVIMSNHRVDLNIKHVLGDVTHKIAKRSSVPVLIVK"
BASE COUNT          228 a          109 c           78 g          281 t
ORIGIN      
        1 gcaacagaac ctatattttg ctataattat taaattaatt gcctttgtgt tagtattccc
       61 tggattacta acactttggt tagctgttct aagtgataca ggtgcagcaa tattagttat
      121 attaaattca ttacgtttat taaaaaaata aaaaagccta aatactctct cgatttgtga
      181 gaaagtattt aggctttttg aattaataaa tttcttactt cttatgttta tttaacaatt
      241 agtactggga cagaacttct tttagcaatc ttatgtgtaa catctcctaa aacatgttta
      301 atattcaaat cgactctatg attactcata attactaaat caaattcacc attatttata
      361 tctccagaag taattttttc taagattgta tctttaatat gcccaaaatc tacatctatt
      421 ttatattgaa tatctctttt ttctaattca cgtaaaaatg gagtgagttt tttctctttt
      481 tctctaataa tatcttcttt atgcttatta taatacttga cactaattgc aagatcattt
      541 tctgttacaa cgtgataaat tacaacaact gaatcttcat cggtgacttt ttctaaatag
      601 tcagggatag cactaaaatc attttcgaaa tcatagggta ataatatatt tttgaacatc
      661 tacaccagcc tcctattact tattatttag atgtct
//
LOCUS       DAMQYT010000404          685 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.412, whole genome shotgun sequence.
ACCESSION   DAMQYT010000404 DAMQYT010000000
VERSION     DAMQYT010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.412"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..549
                     /locus_tag="PGA33_002873"
     CDS             <1..549
                     /locus_tag="PGA33_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6474594.1"
                     /translation="LSFSLLTFPLDIINEKQNEWRQRLMVTPFTFTSYYISKVVKTML
                     QFAIAILVIFMVGHFYKGVAMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQKTSA
                     LANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLGVDIASNHHINLISFAI
                     ILLYALGSIIAVYCISHFKRAE"
     gene            553..>685
                     /locus_tag="PGA33_002874"
     CDS             553..>685
                     /locus_tag="PGA33_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDG6474595.1"
                     /translation="MKFLKDTSIAEISSIVYLIFPIAGIFFNEVYGPKWLYIISVIVF
                     "
BASE COUNT          215 a           83 c          114 g          273 t
ORIGIN      
        1 ttaagtttta gtttactaac ttttccatta gatattatta atgaaaaaca aaatgaatgg
       61 cgccaaagat taatggtaac accatttact tttactagtt attatatttc aaaagtagtg
      121 aaaactatgc tgcaatttgc aatagcgata ttagttattt ttatggttgg acatttttat
      181 aaaggtgttg caatgagtgc agttcaatgg ttagagtcag gaatattttt atggttaggt
      241 gcgtctctat taataacttt tggcatatta ttttctttgt taaatgatat tcaaaaaaca
      301 agtgctttag ctaatatcgt aacaattggt ttagcagtat taggtggatt gtggtttccg
      361 ataaacacat ttccaaattg gcttcaacat gttgctcatg ttttaccgag ctatcatttg
      421 cgtaaactag gtgtagatat tgcttcaaat catcatatca atttaatatc atttgctata
      481 atactcttgt atgctttagg gagtataata gcagtatatt gtattagtca ttttaaaagg
      541 gcggaataaa atatgaaatt tttaaaagat acttcaattg ctgaaatatc gtctatagtt
      601 tatctgattt ttcctattgc cggtatattt tttaatgaag tatatggtcc caaatggttg
      661 tatattatat cagtcattgt ctttt
//
LOCUS       DAMQYT010000405          668 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.414, whole genome shotgun sequence.
ACCESSION   DAMQYT010000405 DAMQYT010000000
VERSION     DAMQYT010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.414"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..617)
                     /locus_tag="PGA33_002875"
     CDS             complement(<1..617)
                     /locus_tag="PGA33_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDG6474596.1"
                     /translation="MEAVVDWVQVTFHIAPISAVIEDVIGLPITLFKKRNSGIYFYNR
                     GYEFSNIKLYYSSDDESMGIHLQLTGTGCREFEHHLQQLNKTWQDFFDKCLSVNANFT
                     RIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRAGSAINGFNLSDGRSKGATFYIGSKQ
                     SNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMRKAYAVNCAKV"
BASE COUNT          218 a          140 c           89 g          221 t
ORIGIN      
        1 actttcgcac aattaactgc atatgccttt cgcatttgta tttcataacg attccatagc
       61 ccaatgtctt cgacatcaca atgacgttta aacgcttgtt cataattctt ttcataaaaa
      121 cgacaatata aattgctttg tttagaacca atataaaaag tagcgccttt acttcttccg
      181 tctgacaaat taaaaccgtt tattgcgctt ccagctctaa aacgtgaaac acactcagct
      241 ttttcagcct ttttaattaa taaaggcact tttaaatatg ttttataatc atcaattgca
      301 atatcaattc ttgtaaaatt tgcattaact gataaacatt tgtcaaaaaa atcttgccaa
      361 gttttattca actgctgtaa atgatgttca aactctctac aacctgtacc agttaactgt
      421 aagtgaatgc ccattgattc atcatcactt gaataataca atttaatgtt tgaaaattca
      481 taaccgcgat tataaaagta aatgccacta tttcgttttt tgaacaacgt tataggcaaa
      541 ccgataacgt cctcaattac tgctgaaata ggggctatat ggaaagtgac ctgaacccag
      601 tcaacaactg cctccacagc acttttattt gtacgctcta cccccctatt agtaaggggg
      661 gtacactg
//
LOCUS       DAMQYT010000406          657 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.415, whole genome shotgun sequence.
ACCESSION   DAMQYT010000406 DAMQYT010000000
VERSION     DAMQYT010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.415"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..305
                     /locus_tag="PGA33_002876"
     CDS             <1..305
                     /locus_tag="PGA33_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDG6474597.1"
                     /translation="FLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQSTGCN
                     AQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVAN"
     gene            283..>657
                     /locus_tag="PGA33_002877"
     CDS             283..>657
                     /locus_tag="PGA33_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDG6474598.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISFITELNYIKSQAIANQGYINVRFYENSDTIKVIENNNIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITI"
BASE COUNT          265 a           76 c          107 g          209 t
ORIGIN      
        1 aatttcttaa gaaaactcaa gcgtttacat tgatagagat gctattagtg ttattaatca
       61 tcagtttatt attaatttta atcattccaa atattgctaa acaaactgct cacatacaat
      121 caacaggttg taatgcacag gtaaaaatgg ttaatagtca aattgaagcg tatgcattga
      181 aacataatag aaatccatcg tctattgaag acttaattgc agatggtttt ataaaagaag
      241 cacaaaagac atgtaaatca ggagagacaa tcacaattag taatggagaa gcagttgcaa
      301 attagaaagc agtcagcatt tactatgatt gagatgcttg tggtaatgat gttaatcagt
      361 atatttctac ttttgacaat gacatctaaa ggattaagca atcttagagt aatagatgat
      421 gaggcaaata tcatttcttt tattactgaa ttgaattata ttaagtcgca agctatagca
      481 aatcaaggat atatcaatgt tagattttat gaaaacagtg acactattaa agtaatagag
      541 aataataata tacgatttct aaaattaaaa gtaggcaaaa taattaatgt tgcaaaagtt
      601 gatattattg cctttgataa aaaagggaat atcaataaat ttggtagcat aacaatt
//
LOCUS       DAMQYT010000407          656 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.416, whole genome shotgun sequence.
ACCESSION   DAMQYT010000407 DAMQYT010000000
VERSION     DAMQYT010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.416"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..365)
                     /gene="lpl4"
                     /locus_tag="PGA33_002878"
     CDS             complement(<1..365)
                     /gene="lpl4"
                     /locus_tag="PGA33_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl4"
                     /protein_id="HDG6474599.1"
                     /translation="MGYLKRLVLYIVIMVMSVFIIGCDKSSDTAEKSKEDSKETQIKK
                     SFAKTLDMYSIKNLEELYDKEGYRDGEFEKGDKGMWTIYTDFAKSNKPGELSNEGMVL
                     YLDRNTRTAKGYYFVRTFYR"
     gene            complement(414..>656)
                     /locus_tag="PGA33_002879"
     CDS             complement(414..>656)
                     /locus_tag="PGA33_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDG6474600.1"
                     /translation="PNYSAEYKMKKNDRNIQQLKKRFNLKTSKTPKLLFKGSGDIKGS
                     SVGYKEIEIIFSRSKEESIYYVDSVEFVPSNKITKS"
BASE COUNT          173 a          137 c           75 g          271 t
ORIGIN      
        1 cgataaaacg tcctgacaaa gtagtacccc ttagctgtgc gtgtgtttct atctaagtac
       61 aagaccatgc cttcattact tagttcaccc ggtttattac ttttggcgaa atctgtatat
      121 atcgtccaca tccctttgtc acctttttca aattcgccat ctcgatagcc ttctttgtca
      181 tatagttcct cgagattctt aattgaatac atatctaacg ttttcgcaaa actctttttg
      241 atttgtgttt ctttcgagtc ttcttttgat ttttctgcag tatcgcttga tttatcacaa
      301 cctattataa aaacactcat aaccataata actatataca atacaagtct ttttaaataa
      361 cccatcattt cacaactctc tttattctca atatttgctt ttgttgacta atatcaactt
      421 tttgttattt tgttacttgg aacgaactca acgctgtcaa cataataaat gctttcttct
      481 ttacttctac taaatatgat ttctatttcc ttatatccta cagaagaccc ctttatatct
      541 ccagatccct taaacaataa ttttggtgtc ttgctagttt ttagattaaa tctctttttc
      601 aactgttgaa tgtttctgtc atttttcttc attttatatt ctgcagagta attggg
//
LOCUS       DAMQYT010000408          655 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.417, whole genome shotgun sequence.
ACCESSION   DAMQYT010000408 DAMQYT010000000
VERSION     DAMQYT010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.417"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            395..499
                     /locus_tag="PGA33_002880"
     CDS             395..499
                     /locus_tag="PGA33_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474601.1"
                     /translation="MFKVNYSILSYYPEYNIAVSWQRLREGKTIKNKI"
BASE COUNT          251 a           76 c          113 g          215 t
ORIGIN      
        1 gaaaaaggag tatgtcgttt catttagaat ttattgcacg aaattagcat cgaataaggg
       61 aaaataagtg ttaagtttta aatgataaaa aagattggaa tggatcgtct tgaaatgctc
      121 ccttcaaagt tttcattttt tcaatgtcga cttcgaaggg ggcattttca ttaaattgtt
      181 atagcttttt atatttgtat aatgaacata taagtttaag aaggtgcgag tgaaggaaat
      241 aaaaaagctc aaatgtacca aattgttaat cttaataaat ctctacttta taaagattga
      301 atggacattc gagcgttaat cagtcaggag ggactttccc tcctacaatt taataataat
      361 acttgcttca ccactataca aggagtgagt tgttatgttc aaagtgaatt attcgatttt
      421 aagttattat ccagaatata atatcgcagt aagttggcaa cgtttaagag aaggaaaaac
      481 aataaaaaac aagatttaat actgctgcgt catgaggcgc ttgaacatta tttgatgaat
      541 aagtataatt tcaactatga ttatgcacat aaaattgtat caaaaaaata cgattattca
      601 atttttataa aaaagaaggt ggattaaatg cttacattaa taaaattgaa agaag
//
LOCUS       DAMQYT010000409          654 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.418, whole genome shotgun sequence.
ACCESSION   DAMQYT010000409 DAMQYT010000000
VERSION     DAMQYT010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.418"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            54..>654
                     /locus_tag="PGA33_002881"
     CDS             54..>654
                     /locus_tag="PGA33_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HDG6474602.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETI"
BASE COUNT          256 a           78 c          110 g          210 t
ORIGIN      
        1 acaaattata actgaatgtg attggtgaca atcgcttaaa tggaggattt taaatgttta
       61 gtaagaaaaa agataagttt atggttcaat tagaagagat ggttttcaat ctggatcgtg
      121 ctgctattga attcggtaaa atggatttca atacacattt agatttaaaa gcatactcag
      181 acaacattaa aacttatgag tcacatggtg acgaattagt acatcaagta attactgatt
      241 taaatcaaac atttatcaca ccaattgaac gtgaagatat tttatcatta tgtgatgcaa
      301 ttgatgatgt tttagatgca attgaagaaa cggcagctat gtttgaaatg tattcaatcg
      361 aatacacaga tgaatatatg gctgagtttg ttgataacat tcaaaaagca gttgcagaaa
      421 tgaaacttgc tgtcggctta ttagtcgata aaaaattatc acatatgcgt attcattcaa
      481 ttaatattaa agaatttgaa acaaactgtg atggtatttt aagacagtca attaaacata
      541 ttttcaatag cgaaacagat ccaatcactt taattaaaat aaaagatatt tatgaaagca
      601 tggaagaaat cgctgataaa tgtcaaatcg tagcaaataa ttttgaaact atta
//
LOCUS       DAMQYT010000410          647 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.419, whole genome shotgun sequence.
ACCESSION   DAMQYT010000410 DAMQYT010000000
VERSION     DAMQYT010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.419"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..280
                     /locus_tag="PGA33_002882"
     CDS             <1..280
                     /locus_tag="PGA33_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDG6474603.1"
                     /translation="LSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPTGALDP
                     KTGQMIIQSLFGLVDENKVLILATHDMAIANQCDEIIDLEQYRKVASM"
     gene            complement(368..634)
                     /locus_tag="PGA33_002883"
     CDS             complement(368..634)
                     /locus_tag="PGA33_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474604.1"
                     /translation="MKIGGVILTRTYNIIGILSCLISFIIMALPMIWYTASALWFFPG
                     AIMILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
BASE COUNT          261 a           89 c          109 g          188 t
ORIGIN      
        1 tctgtcaaac agtttaaaaa gaaaagttca tacgctaagt ggaggtgaac aacaacgtgt
       61 cgctttaatt agaatgatgt taaaagatcc gattgttatg ttagctgatg aaccaacggg
      121 tgcgttagat cctaaaacag gacagatgat tattcaatca ttatttggtt tggtcgatga
      181 aaataaagtg ctgattttag caacacatga tatggctatt gcaaatcaat gtgacgaaat
      241 aatagattta gaacagtata gaaaagtagc atctatgtga tatccaaaag ataaaattaa
      301 aagccaatca atgggaaatg ttgacttcaa cactccgttt gattggcttt tatactgtgt
      361 tactaagtta tgaaagtaac aacggcaaac tgaaaaaaag caatataata atatttaata
      421 cgattagtaa taaatgtaat tgatttttcg ttttaatata gcaaaaaaca atgactaaag
      481 ataacaacaa tatcatgatt gcacctggga agaaccataa tgctgaagct gtgtaccaaa
      541 tcattggtaa cgccataata ataaaagata taagacaaga aaggatacca ataatattat
      601 aagttctagt caaaattaca cctccaattt tcactttaaa atgacat
//
LOCUS       DAMQYT010000411          644 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.420, whole genome shotgun sequence.
ACCESSION   DAMQYT010000411 DAMQYT010000000
VERSION     DAMQYT010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.420"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            69..446
                     /locus_tag="PGA33_002884"
     CDS             69..446
                     /locus_tag="PGA33_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDG6474605.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
BASE COUNT          250 a           88 c          113 g          193 t
ORIGIN      
        1 aattagtcca ccatttcgat ttgtgctatg ataatagtgt taagtaaacg aaataagggg
       61 ttattaagtt gaataactac aaaattggcc aacatatcaa ggtgcgtgta actggtattc
      121 aaccatacgg tgcgtttgtt gagaccccta atcatactga aggactgatt catatatcag
      181 aaattatgga tgactacgtt cataatttga agaaatttct atcagaaggc caaattgtta
      241 aagctaaaat tttgtctata gatgatgaag gaaagcttaa tctatcatta aaggataatg
      301 attacttcaa aaattatgag cgtaagaagg aaaaacaatc agtattagat gaaatcagag
      361 aaacagaaaa atatgggttt caaacactta aagaacgctt accaatctgg ataaaacagt
      421 caaagcgagc aattcgaaac gactaaagga acagataaat cgtaccgaaa atcatacaaa
      481 gggtctgaaa tgaaagtttc ttagactata aaagagatta gtatctatta aattttatta
      541 gatactaatc tctttttgtc tacgataacg taatatgatt gattctattt acacgtacaa
      601 atggtttaag gtgacatatc cattatcttt gttagataga atcg
//
LOCUS       DAMQYT010000412          641 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.421, whole genome shotgun sequence.
ACCESSION   DAMQYT010000412 DAMQYT010000000
VERSION     DAMQYT010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.421"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..563)
                     /locus_tag="PGA33_002885"
     CDS             complement(<1..563)
                     /locus_tag="PGA33_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDG6474606.1"
                     /translation="MLCETENINKNPKYRIIKYKDEYLMIDLVSTWLALFFPMINWLI
                     PKKYVKISEKDFETLNIVKTAKINSFWPVAGSTVLFGVMLRRYSHLFIVKYEYSIVIL
                     ICCIIILGIFLFFLYLNQKLKLQIYNENKNKSNKIIIFPTLKSLCLSIVLYIYLGGGS
                     FFTIYMLLTIEVQNIILFITLFVIFLF"
BASE COUNT          247 a           93 c           66 g          235 t
ORIGIN      
        1 aaaaacaaaa atattacaaa caaagttata aataatatta tattttgaac ttcaatcgtc
       61 aataacatat aaatagtaaa gaatgaacca ccacctaaat aaatatataa aactattgat
      121 aaacaaagac tctttaaagt gggaaatatg attatcttat tgcttttatt tttgttttca
      181 ttatagattt gtaactttaa cttttgattt aaatataaaa aaaacagaaa aatacctagt
      241 attatgatgc aacaaattaa aattactata ctatattcat atttaacgat aaataaatgg
      301 gaataccttc ttaacataac accaaataag accgtacttc ctgccactgg ccaaaaagaa
      361 ttgattttag ctgtcttcac aatgtttaaa gtttcaaaat ctttttcgct gattttgacg
      421 tacttttttg gaatcagcca attaatcatt gggaaaaaga gtgctaacca tgtacttact
      481 aaatcaatca tcaaatattc atctttgtat ttgataattc tatatttggg attcttatta
      541 atattttcag tttcacaaag caatgtctcc acttcttttc aattggtttt atattgatac
      601 actttcatat acattagaag tcatttaaat tctactagcg t
//
LOCUS       DAMQYT010000413          636 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.422, whole genome shotgun sequence.
ACCESSION   DAMQYT010000413 DAMQYT010000000
VERSION     DAMQYT010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.422"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(237..422)
                     /locus_tag="PGA33_002886"
     CDS             complement(237..422)
                     /locus_tag="PGA33_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDG6474607.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
BASE COUNT          234 a           91 c           86 g          225 t
ORIGIN      
        1 caacatgaag catttaattt tacagtgatg attataaaat aattgccttg atacaaagat
       61 tactcgtaaa tgacatcttt gtattaaggc tttttctaaa tttaaaagtg atgggttaga
      121 ggtcattgag ctttaaaata ttcaaaatac aaaacattaa tggccaaaaa taaaagccgc
      181 ctttatctgg gcagcttcaa taataagaaa gacatatttc attttatact aaatagttat
      241 tgtgatgaat ctttcggcgg tttaattact gcagcaaaaa ttgctgtgaa aatcgtgaac
      301 aatactgcca tgataattgg attcactaca tttaagctgt ctccacctac taggctatta
      361 agtacaaagt taaccatttg cattaataat aatgcccaaa agaatgttac gaggtgtttc
      421 atgtcattct acctccactt taattatata tattttattt taagtgaaag ttagaaattt
      481 gtatagtaac atctcatata ttttgaccat attatacagt ttaaataaat gattttatct
      541 gaatggctat tctaaattaa gcgcattaaa accaatttca tactgaaatt tgacgataat
      601 aaagcattaa aattttatta actagtcaat attcct
//
LOCUS       DAMQYT010000414          629 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.423, whole genome shotgun sequence.
ACCESSION   DAMQYT010000414 DAMQYT010000000
VERSION     DAMQYT010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.423"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            121..629
                     /locus_tag="PGA33_002887"
                     /pseudo
     CDS             121..629
                     /locus_tag="PGA33_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          237 a           96 c           91 g          205 t
ORIGIN      
        1 aaaataagac atttttccca acttgcacat aaatgtaaaa ttcaataaaa tgaattttct
       61 gtgttgggtc ccttcgtata atttaataaa tactactaaa ctaaattaaa gaggtgcctt
      121 atgtataaaa attataacat gaccccaact tacactacca atagaaactt cgttagaatt
      181 cctcaaaatg atatttcgcg atatgttaat gaaattgttg aaacgatacc tgatagcgaa
      241 ttcgatgaat tcagacatca tcgtggtgca acatcctatc atccaaaaat gatgttaaaa
      301 atcatcttat atgcatatac tcaatctgta ttttctggtc gtagaataga gaaattactt
      361 catgacagta ttcgaatgat gtggttagcc caagatcaaa cactttctta taaaactatt
      421 aatcgtttta gagtgaatcc taatactgat gcgttaattg aatctttatt tattcagttc
      481 tatagtcaat gtttaaagca aaatcttatt gatgataatt caatttttat tgatggtaca
      541 aaagtagaag ctagtgccaa tagatataca ttggtgcgga agaaagtatt caaaatcacg
      601 aatcgaaatt gaacgagaat tcaaaaaca
//
LOCUS       DAMQYT010000415          628 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.424, whole genome shotgun sequence.
ACCESSION   DAMQYT010000415 DAMQYT010000000
VERSION     DAMQYT010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.424"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            35..178
                     /locus_tag="PGA33_002888"
     CDS             35..178
                     /locus_tag="PGA33_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HDG6474608.1"
                     /translation="MEKVLDLDVQVKGNNNTNDSAGDERITSHLFCSFGCEKTGSFNS
                     FCC"
BASE COUNT          213 a           83 c           98 g          234 t
ORIGIN      
        1 cttaacttca acttttaaat agggggtttt atctttggaa aaagttcttg atttagacgt
       61 gcaagttaaa ggaaataaca acactaatga ttcagcgggt gacgaaagaa taactagcca
      121 tcttttttgt agctttggtt gtgaaaagac gggtagtttt aacagcttct gttgttaagg
      181 atacagcaaa tagagcctga aagggctcta tttgtctttt taaatatatt aagtgctaaa
      241 aaacggaaac agtttattaa tcgccaggta tttttataat tagccttcta tattatcagt
      301 tgagtgtttc aataaattct gactttggtg atggaaagtt atacttttag ttgtaatata
      361 gttttcatga cttcctgtta aataagttag gattatgtaa ttagcctcta tctttacata
      421 taagtaatga caagctttgg ttattatatt gaccctctta gaacatgtca tttaagaaaa
      481 taatcaagtc attgaaatta tatgtttata tcctcatctt taaaataact tcaattatta
      541 tttgctaaat tttgtataaa tagaacaaaa ctgcataaat atctattaat taatctcaag
      601 cagccatctc caaattcgta atagtatt
//
LOCUS       DAMQYT010000416          627 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.425, whole genome shotgun sequence.
ACCESSION   DAMQYT010000416 DAMQYT010000000
VERSION     DAMQYT010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.425"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            65..382
                     /locus_tag="PGA33_002889"
     CDS             65..382
                     /locus_tag="PGA33_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HDG6474609.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
     gene            complement(530..>627)
                     /locus_tag="PGA33_002890"
     CDS             complement(530..>627)
                     /locus_tag="PGA33_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474610.1"
                     /translation="NKEFAKSNLSLNNEKWKFVCSKSKHFFKKSS"
BASE COUNT          234 a          103 c           83 g          207 t
ORIGIN      
        1 aataatataa attaaggtat agtaaagcta actcagaaat gacttatcat tcggaggtta
       61 cattatgaat aaactattac agtcattatc agccctcggt gtttctgcta cactagtaac
      121 accaaattta aatgcagatg caacgacgaa tactacacca caaattaaag gcgctaatga
      181 tatcgttatt aagaaaggtc aagattataa ccttctaaac ggcataagtg catttgataa
      241 agaagatgga gatttaaccg ataaaattaa agtcgatggc caaattgata catctaaatc
      301 tggtaaatat caaattaaat atcatgtcac tgattcagat ggtgcaatta aaatttccac
      361 taggtatatt gaggttaaat agccctcatc actatactgc aaataaaatg gtagcaaacg
      421 aacatgtttt gctaccattt tatttgttat tctaacttca tctgcaactt taacccaaat
      481 attgtatttt ttctgtatac caaaggacta cctatcaaat tattaaaact taactgctct
      541 ttttaaaaaa atgttttgat tttgaacaaa caaatttcca cttttcattg tttaacgata
      601 aattactttt ggcaaattcc ttattaa
//
LOCUS       DAMQYT010000417          627 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.426, whole genome shotgun sequence.
ACCESSION   DAMQYT010000417 DAMQYT010000000
VERSION     DAMQYT010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.426"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..554
                     /locus_tag="PGA33_002891"
     CDS             <1..554
                     /locus_tag="PGA33_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474611.1"
                     /translation="ILQNNLDLFFNYGKRLANDHYNTLISKSKERLINKAHDEIQPFN
                     HLDLNSMKEIVDDITFSYQIEQGLQAYKRELYLPAAATFAVAIETFLIKLKKVNKIKH
                     KDTDSTMYTKLLGELTKEGKVNYRTKKRVEIAYSMRNIINHSQAGAVAKGDCDFLLNT
                     LKDIVDENEKILREYTKSINKTE"
     gene            complement(534..>627)
                     /locus_tag="PGA33_002892"
     CDS             complement(534..>627)
                     /locus_tag="PGA33_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDG6474612.1"
                     /translation="EQELKLKIYEQIMLKIKSEYKDTYLFRLIN"
BASE COUNT          265 a           79 c           92 g          191 t
ORIGIN      
        1 taatattaca aaacaattta gatttatttt ttaattatgg caaaagatta gctaacgatc
       61 attacaatac attgatatcg aagtcgaaag aaagattgat aaacaaagca catgatgaaa
      121 tacaaccgtt caaccactta gatttaaata gtatgaaaga gattgttgat gatataactt
      181 tttcttatca aatagaacaa ggattacaag cttataaaag ggaattgtat ttgccagctg
      241 cagcaacctt tgctgttgct atagaaacgt ttttaatcaa attaaaaaaa gttaataaaa
      301 tcaaacataa agacaccgat tcaactatgt acacaaaatt attaggagaa ttaactaaag
      361 aaggtaaagt aaattataga accaaaaaac gggtagaaat tgcgtatagt atgagaaaca
      421 taatcaacca ttcacaagct ggtgcagtag ccaaaggtga ttgtgacttt cttttaaaca
      481 cactaaaaga cattgttgat gaaaacgaaa aaatattaag agaatatacc aaatcaatta
      541 ataagacgga ataaataggt atccttgtat tcagatttga tttttaacat aatttgttca
      601 taaattttta atttaagttc ttgttca
//
LOCUS       DAMQYT010000418          611 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.428, whole genome shotgun sequence.
ACCESSION   DAMQYT010000418 DAMQYT010000000
VERSION     DAMQYT010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.428"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(56..410)
                     /locus_tag="PGA33_002893"
                     /pseudo
     CDS             complement(56..410)
                     /locus_tag="PGA33_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373826.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            complement(472..609)
                     /locus_tag="PGA33_002894"
     CDS             complement(472..609)
                     /locus_tag="PGA33_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474613.1"
                     /translation="MILIMLDSQLDGNVTAKELSESFKELVEEFESIEKAKKNSNSKD
                     K"
BASE COUNT          164 a          111 c           65 g          271 t
ORIGIN      
        1 tggctctttt tttattttca ttatataggt gatgacctaa catatatcta ttttattttt
       61 ccgcaattta tttctatttt tattttacca tttttaatca tttctttcat ctggttttta
      121 gctttttcct gtaattatgc ttcttttact tctacttgat atttaccttc acgctcttta
      181 aagaacttgt cccgccaatt gccaacatgt ggcactgtgg tacttctaca ccaaggatgc
      241 ataggtggcg cattcacacc tggtatcata tctttaactt taaatatttt tccgttaagt
      301 gaatgacata atttagatgt tttactatct attttggcaa catatttata ttcgccatct
      361 tcaccaagtt cttttaaata tgttaacttt tgtgcttctg cattttcagt aaatagttaa
      421 aaaagcgtat aaaaatagca ccactttctc tttatctgtc taaaaaggat gctatttatc
      481 ttttgaattt gaattctttt tcgctttttc tatactttca aattcttcaa ctaattcttt
      541 aaaagattca ctcaattctt ttgcagttac atttccatct aattgtgaat ctaacataat
      601 taaaatcatc t
//
LOCUS       DAMQYT010000419          601 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.429, whole genome shotgun sequence.
ACCESSION   DAMQYT010000419 DAMQYT010000000
VERSION     DAMQYT010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.429"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
BASE COUNT          262 a           76 c           81 g          182 t
ORIGIN      
        1 aaatactgag tcattcaatc atcaagacat tttaacaagt gatgaaaaag agttaataaa
       61 attacgcaaa gaaaaacatc gattgataat gtaaaatgat gatttaaagc aagcagtgct
      121 aattttgaga caaaaaatga agtctttcaa caaaatgcgt atcaatattc agtatcagca
      181 atgtgcaaag ttctaaaaat ttcaagaagt acctattatg attctaaaaa aggagaatat
      241 tacaactaga tacaaaaagc ctcaatcaca accaagtaac taaaacgtaa aaattaatta
      301 gattaattat tatctgcata acaaatgacc atatacatca actagagaca gactatttaa
      361 tcatttacaa tgtcctattc gttttagacc atgttcaaat gaatagtgtt tataaaatgt
      421 tgacactgta gtggcatatt gtaaattaaa tattattatg tgatcatttt gtttaaatat
      481 tagttataag aagtgaaata ttcattttaa aaggagactt aaaacttgaa acgatcaacg
      541 aatcaagaaa aatttcttga tacactaatt agacttaata ctaagattga agaactagga
      601 a
//
LOCUS       DAMQYT010000420          601 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.430, whole genome shotgun sequence.
ACCESSION   DAMQYT010000420 DAMQYT010000000
VERSION     DAMQYT010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.430"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            6..305
                     /locus_tag="PGA33_002895"
     CDS             6..305
                     /locus_tag="PGA33_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HDG6474614.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            223..>601
                     /locus_tag="PGA33_002896"
     CDS             223..>601
                     /locus_tag="PGA33_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDG6474615.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDILDDFKGVDRNDIEIRQHRIILH
                     KGEEMIEYKLINNKIIKVVNDRGN"
BASE COUNT          238 a           68 c          101 g          194 t
ORIGIN      
        1 tcgttatgaa aagctataag tgtaaaggtt cattcttaat agatagtatg gctggatttt
       61 tgctaattgg attgattaca ttactattga taccaatgat gaatcaaatg caagcgagta
      121 taaaccataa actacaaaca attgatgctt ctaaagtaat tttgacgact gtatctaaaa
      181 ttaataaaga agaacttaag aagggggtaa ctatagggaa gtatgatatt aagcaaagtg
      241 accaacaaat ttgtgctatt tcaaaaaata ccacttctta tcaaaagaca tgtatacagt
      301 attaatgtca aagctttttc gctcattgaa atgttagtag cgatgatggt tataagtata
      361 actttactaa ttgttccaga cttaattaga cttagtaaaa cttttctaat tgaaagtagg
      421 gatttaacaa ctgtagattt cgaatttttc tcaagagata ttctagatga ttttaaagga
      481 gtagatagaa acgatattga aattaggcaa caccgtatca ttttacataa aggtgaagaa
      541 atgatcgaat acaaattaat taataataaa attattaaag ttgtaaatga cagaggaaat
      601 a
//
LOCUS       DAMQYT010000421          587 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.431, whole genome shotgun sequence.
ACCESSION   DAMQYT010000421 DAMQYT010000000
VERSION     DAMQYT010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.431"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..226
                     /locus_tag="PGA33_002897"
     CDS             <1..226
                     /locus_tag="PGA33_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDG6474616.1"
                     /translation="QMIEFLGIESLKKHLKNSGGIALLPEFIVADELENKSLFQIDVD
                     VDIPNLETTLIINPESNKQVLESFVKDVFL"
BASE COUNT          218 a           56 c           94 g          219 t
ORIGIN      
        1 acaaatgatt gaatttctag gaattgagtc attaaaaaaa cacttgaaaa attcaggtgg
       61 gattgcgcta ttgccggaat ttattgttgc agatgaatta gaaaataaaa gtttgtttca
      121 aatagacgta gatgtcgata ttccgaattt agaaacaaca ttgataatta atcctgaatc
      181 gaataagcaa gtacttgaat cttttgtaaa agatgttttt ttataattat tggtgaaaac
      241 gtgtagttat ggtgaaactc aaagataata atttaaatga gatgttaatg aaaaagtaat
      301 tcaatataaa aacaggtgat ttatatcttt aataaggatt atttctagtt gaaatttcaa
      361 attgcgggca ttcattttca aaatagtaaa gaatttaata gatgaaaacc tttgaatcat
      421 actagttagt atggattgat taatatttta acctgctaaa tgagtagctt atgtatttta
      481 aatagctgtc atctagcagg ttctttatta ttaataagtt cagataaatt attttagtct
      541 gttttctaat gttgataata ataactttaa atctgtactt tcattgt
//
LOCUS       DAMQYT010000422          585 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.432, whole genome shotgun sequence.
ACCESSION   DAMQYT010000422 DAMQYT010000000
VERSION     DAMQYT010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.432"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            42..224
                     /locus_tag="PGA33_002898"
     CDS             42..224
                     /locus_tag="PGA33_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDG6474617.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKVIGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            264..410
                     /locus_tag="PGA33_002899"
     CDS             264..410
                     /locus_tag="PGA33_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474618.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
BASE COUNT          269 a           59 c          101 g          156 t
ORIGIN      
        1 cttagtggta tataaatata tacatccaag gaggtttcac tatggcagac gaaagtaagt
       61 tcgatcaatt taaaggaaat gttaaagaaa cagtaggtaa cgttactgat aacaaagaat
      121 tagaaaaaga aggtcagcaa gataaagtga ttggtaaagc aaaagaagtt gttgaaaatg
      181 ctaaaaacaa aataactgat gcaattgata aacttaaaaa ataagttcaa ttaaactaat
      241 caaatataag aggagcgatt aatatgagtt ttatggataa agctaaagac gcagtagaaa
      301 aatttaaaaa tagcgataat gaacaagtta aaaatgtaaa agataagata aatgaatata
      361 caggatcgaa taacgaagag aaaaaagaaa atgaagataa agagaaataa atttctttaa
      421 attttggata tgcttttcaa tattttgaaa gcgccccatc aaataaacac ttacacagtg
      481 taaagttgaa ggggcgcttt catatttgca ttagaatttt aagtgaattt gaatcattac
      541 taaaataaat gattgaacaa taaagacatc tttcattcaa aatgc
//
LOCUS       DAMQYT010000423          579 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.433, whole genome shotgun sequence.
ACCESSION   DAMQYT010000423 DAMQYT010000000
VERSION     DAMQYT010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.433"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(26..259)
                     /locus_tag="PGA33_002900"
     CDS             complement(26..259)
                     /locus_tag="PGA33_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474619.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
BASE COUNT          206 a           87 c           55 g          231 t
ORIGIN      
        1 tatgattcta aatgcttttt aatatttaat gctcatcaac atttggattt tgaatattca
       61 attcaaaaac tttattagct acgtcaattg taaaatcaga accatagttg acatgagcta
      121 cttttaattt tccatctaaa taatagattg cgattgcaac atcgtaaaat tcgtcaatga
      181 caaataaact cttttcgttt gttacaacct catgctctcc tgagtataca acgttaattt
      241 cccaatcatt aaaaaccatt tgttaacctc cttgaacatt taaattgatt caacttaagt
      301 ttaacttatt catacaactt cgtacaatat ctagatgaac attaattgta tttctagaaa
      361 tctttttcaa ttatatgtac taattatact tttaaatttc ttatttcagt atagttttaa
      421 aacgatttta aaataattct gcaaatatat taacacataa tgtgttcaaa aagttttgaa
      481 caatttcaaa acttttatat aaagggtttg acaacatgga attcaaattt cttattttaa
      541 aaattacctc atatagtgtc atgtttagtc taattttta
//
LOCUS       DAMQYT010000424          578 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.434, whole genome shotgun sequence.
ACCESSION   DAMQYT010000424 DAMQYT010000000
VERSION     DAMQYT010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.434"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            8..181
                     /locus_tag="PGA33_002901"
     CDS             8..181
                     /locus_tag="PGA33_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474620.1"
                     /translation="MNKKAAKLLEDYKKVQQRKSKETDRGCLKSVIMLIVLFFLIIFG
                     IVACSTNVNFFFQ"
BASE COUNT          185 a           97 c           99 g          197 t
ORIGIN      
        1 taaacatata aataaaaaag ccgcaaaact acttgaagat tataaaaagg ttcaacaaag
       61 aaaatcgaaa gaaactgata gaggttgttt gaaaagtgtc atcatgctta tcgtcttatt
      121 tttcttaatt atttttggta ttgtggcatg ttctacaaac gtaaactttt tcttccagta
      181 aaagagtgag atagaaatga taaagaacca ctaatgattt attatgtagt ggttctttat
      241 cattagctgt agctaatgtg tacttaaaaa atgaatacat gagtaaagct catgcataag
      301 aaatactaat tgctaaagaa aaagtatttc tttatcgatg tcctacccca acttgcattg
      361 tctgtagaaa ttggggttcc aatttctcta tgttggggcc ccgccaactt gcacattttt
      421 gtaagctgac ttttcgccag cttctatatt ggggcccccg ccaacttgca ttgtttgttg
      481 gaattggtga tcctatttct ctctgttgtg gccccacgag tattgaaaaa acttgttata
      541 agcttatttt catttagtca actactacca atatagca
//
LOCUS       DAMQYT010000425          565 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.435, whole genome shotgun sequence.
ACCESSION   DAMQYT010000425 DAMQYT010000000
VERSION     DAMQYT010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.435"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..221
                     /locus_tag="PGA33_002902"
     CDS             <1..221
                     /locus_tag="PGA33_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474621.1"
                     /translation="DFFEKWSFILNLILFCAFLFLVVLMFYKDVDLIYIFCTLLISLL
                     PLLNIIKRIGWEINQSSMIYEVILAVID"
BASE COUNT          187 a           86 c          106 g          186 t
ORIGIN      
        1 gagatttttt tgaaaagtgg tcgtttattt taaatttaat tttattttgt gcttttttgt
       61 ttttagtggt tctgatgttt tataaggatg tcgatttgat ttatattttt tgtacattat
      121 taatatcatt attacctttg ctaaatatta taaaaagaat aggctgggaa ataaatcaat
      181 cttctatgat ctacgaagtt atattggcag taattgactg aacgaaaatg cgcttgtaat
      241 aagctttttt caattctagt caggggcccc aacacagaga atttcgaaaa gaaattctac
      301 aggcaatgcg agttggggcg gggccccaac aaagagaaat tggattccca atttctacag
      361 acaatgcaag ttggggtggg acggcgaaat aaattttgcg aaaatatcat ttctgtccca
      421 ctccccagaa aaggttctac cattgtcaaa aaaatgcatc tctacatgtt agagtaaata
      481 ttggtcagcc aaccaaaata atcaacacga ggagatgcta tttaatgtca tctgacacaa
      541 acagtttagc acatacgaaa tggaa
//
LOCUS       DAMQYT010000426          564 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.436, whole genome shotgun sequence.
ACCESSION   DAMQYT010000426 DAMQYT010000000
VERSION     DAMQYT010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.436"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            24..533
                     /locus_tag="PGA33_002903"
     CDS             24..533
                     /locus_tag="PGA33_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDG6474622.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
BASE COUNT          245 a           77 c          107 g          135 t
ORIGIN      
        1 aacaagtaaa aggagaatct attatgactg tagataacaa taaagcaaaa caagcatacg
       61 acaatcaaac tggtgttaac gaaaaagaaa gagaagagcg tcaaaaacaa caagaacaaa
      121 atcaggagcc tcaattcaaa aacaaattaa cattctctga tgaagttgtt gaaaaaattg
      181 ctggtatcgc tgcacgtgaa gttaaaggta tcttagatat gaaaggtggc ttaactgata
      241 cattcactaa tgcattctca agtggcaaca atgttactca aggtgtatct gttgaagttg
      301 gtgaaaaaca agctgctgta gacttaaaag taatcttaga atatggtgaa tcagcaccaa
      361 aaatcttccg taaagtaact gaattagtta aagaacaagt taaatatatt actggtttag
      421 atgtagttga agttaacatg caagttgacg atgtaatgac tcaaaaagag tggaaacaaa
      481 aacacgaaaa aaataacgaa aacaataacc aagaaagaca aggtttacaa taagttattc
      541 gataaaaagg gcttgggaca ttaa
//
LOCUS       DAMQYT010000427          562 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.437, whole genome shotgun sequence.
ACCESSION   DAMQYT010000427 DAMQYT010000000
VERSION     DAMQYT010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.437"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            215..>562
                     /locus_tag="PGA33_002904"
     CDS             215..>562
                     /locus_tag="PGA33_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDG6474623.1"
                     /translation="MKLKSFITVTLALGMIATTGATVAGNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKE"
BASE COUNT          223 a           69 c           92 g          178 t
ORIGIN      
        1 aagaagtaat taaatttaag aaatgtaaac ttagttattg taatgtgaat ttatttgaaa
       61 aaatagaaag tattaacaat tatagctttt acattaatta aaatttattt ttaaaaacaa
      121 gtaaacaatt tacatactta taatttttga aacttttcaa tttgtgttat attgattttg
      181 taagatactt taactcacaa aggagagaga ttatatgaaa ttaaaatcat ttataactgt
      241 aactttggca ctgggcatga tcgcaacgac tggcgctact gtggcaggta atgaggtatc
      301 tgcagcagaa aaggacaaac taccggcaac tcaaaaagct aaagaaatgc aaaatgttcc
      361 atatacaatt gcagtagatg gcattatggc tttcaatcaa tcttacttaa atttaccaaa
      421 agatagccaa ttatcatatt tagatttagg aaataaagtt aaagctttgt tatatgatga
      481 acgcggtgta acacctgaga agattcgaaa tgcaaaatct gccgtttaca cgattacttg
      541 gaaagatggt agtaaaaaag aa
//
LOCUS       DAMQYT010000428          525 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.438, whole genome shotgun sequence.
ACCESSION   DAMQYT010000428 DAMQYT010000000
VERSION     DAMQYT010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.438"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            202..>525
                     /locus_tag="PGA33_002905"
     CDS             202..>525
                     /locus_tag="PGA33_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDG6474624.1"
                     /translation="MTFEEKLSEMYSEIANKISSMIPVEWEKVYAMAYIDDGGGEVFY
                     YYTEPGSNELYYYTSVLNKYDISESEFMDSVYELYKQFQNLRNLFKEEGHEPWTSCEF
                     DFTREG"
BASE COUNT          202 a           51 c          110 g          162 t
ORIGIN      
        1 gcaaattgca attatcgtaa caataattgc ttttgtttta aatttattag catatttagg
       61 tttaagataa gtaaaacgaa tttgaagaag cattaagcga catttgggtg ttgtttaatg
      121 ctttttattt aacggaaata agtgtagaga ataaattaat agtttaatca agagatattt
      181 cgaacacata ggggcgataa catgactttc gaagaaaaat taagtgaaat gtatagcgag
      241 attgcgaata agattagcag catgataccg gtagagtggg aaaaggtata tgcaatggca
      301 tatatagatg acggaggagg agaagtgttc tactattaca cagaacctgg aagtaatgaa
      361 ttatactact atactagtgt attaaataaa tatgatatat cggaatcgga atttatggat
      421 tcagtgtatg agttgtataa acaatttcaa aatttaagga atttatttaa agaagaagga
      481 catgaaccat ggacatcatg cgaatttgac tttacaagag aaggt
//
LOCUS       DAMQYT010000429          521 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.439, whole genome shotgun sequence.
ACCESSION   DAMQYT010000429 DAMQYT010000000
VERSION     DAMQYT010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.439"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..>521
                     /locus_tag="PGA33_002906"
     CDS             <1..>521
                     /locus_tag="PGA33_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDG6474625.1"
                     /translation="LHIKEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDGTRTSR
                     EKLKEYMEIISKYQVRTVTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGL
                     ATANTLAAILNGAKQIETTFLRIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTS
                     INISKILDFQISE"
BASE COUNT          211 a           63 c           84 g          163 t
ORIGIN      
        1 acttgcatat aaaagaaaag cttaattttt caaataaata ttatattcag aaaatcaaag
       61 actgcttgga tatattaaag aaagataaaa aaggagtaga tatttgtttt gaagatggaa
      121 caaggacttc tagagaaaaa ttgaaagaat acatggaaat tatttcaaaa tatcaagtta
      181 gaacagttac atttgcggac actgtaggat gttcgacacc attagaatac ggagatattt
      241 ttaattactt tgtaaaaaaa tattctaaca taattttttc tgctcattgt cataacgatc
      301 tagggttggc tactgcaaat acattagctg caattttaaa tggtgcaaag caaatagaaa
      361 ctacattttt gagaattggt gagagagcgg gtaatgctcc tattgaggaa ataattacta
      421 ttttgacaaa aaaacaaata gaaagtacgg aattcacttt acccgacgta tataaaacta
      481 gtattaatat ttctaaaatt ctcgattttc aaatatcaga a
//
LOCUS       DAMQYT010000430          515 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.440, whole genome shotgun sequence.
ACCESSION   DAMQYT010000430 DAMQYT010000000
VERSION     DAMQYT010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.440"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..>515
                     /gene="pmtB"
                     /locus_tag="PGA33_002907"
     CDS             <1..>515
                     /gene="pmtB"
                     /locus_tag="PGA33_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HDG6474626.1"
                     /translation="LTLYPFYIMLDSTKFFYLLQSFISPTILIIWILDAGHLFRLNRR
                     LGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGTLVISLYAYEADVIEPNSIYFST
                     AYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTIIILVPFLFSIAIVLVNYFVLSQ
                     SSFPDLYSYIL"
BASE COUNT          156 a           74 c           66 g          219 t
ORIGIN      
        1 tattaacctt atatcctttt tatataatgc ttgactcaac aaagttcttt tacttattgc
       61 aatcttttat aagtccaaca atattgatta tttggatttt agatgctggt cacttattta
      121 gactcaatcg tagattaggt ggtaatgatt catattattt ttatatgagt ttaccagtgt
      181 caaaaaaaca attgcttaat gccaactata ttacatgcat tgttttaaca ttaattggaa
      241 cgcttgttat tagtttatat gcttatgaag cagatgtgat tgaaccaaat tcaatctatt
      301 tttcaactgc atatgcattt gtcatatcta acttcttgtc tataccaatt gcatttagtc
      361 aatttacaga attgcgtaga gtcaaagtgc catatggtat atacgtgttt actattatca
      421 ttttagttcc atttttattt tcaattgcaa tagtattggt gaattatttt gttctaagtc
      481 aatcatcatt cccagattta tattcatata tttta
//
LOCUS       DAMQYT010000431          506 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.442, whole genome shotgun sequence.
ACCESSION   DAMQYT010000431 DAMQYT010000000
VERSION     DAMQYT010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.442"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            271..506
                     /locus_tag="PGA33_002908"
                     /pseudo
     CDS             271..506
                     /locus_tag="PGA33_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          204 a           74 c           68 g          160 t
ORIGIN      
        1 tgtaatttta ctttgaaata ctttaaaaaa ataagacact ttgcccaact tgcacataaa
       61 tgtaaaattc aataaaataa attttctgtg ttggatccct tcgtataatt taataaatac
      121 tactaaacta aattaacgag gtgccttatg tataaaaatt ataacatgac ccaacttaca
      181 ctaccaatag aaacttctgt tagaattcct caaaatgata atacgcgata tgttaatgaa
      241 attgttgaaa cgatatctga tagcgaattc gatgaattta tatgtcctaa taacaaaaga
      301 ataggtttta aaagatatgc ataccgtaat gatagatata gttttaaacg tgacttcaag
      361 ctatatgaat gtgatgactg ttcatcatgt tctttgagac atcaatgcat gaagccaaat
      421 tcgaaatcca ataagaaaat tatgaagaat tataattggg aatactttaa agcccaaatt
      481 aatcaaaagc tttctgaacc agaaac
//
LOCUS       DAMQYT010000432          506 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.443, whole genome shotgun sequence.
ACCESSION   DAMQYT010000432 DAMQYT010000000
VERSION     DAMQYT010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.443"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..404)
                     /locus_tag="PGA33_002909"
     CDS             complement(<1..404)
                     /locus_tag="PGA33_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDG6474627.1"
                     /translation="METLLCESKVINKNPKYRIIKYDSEYLMIDLASNWIVFFFPFIN
                     WLIPKTYVKITKNDYEKLNIVKPVKNKSIGWTIFAGIVLLGGTVRRNTYLFDFQLEEL
                     IVWSSCFIGFLEIIFFYCYLNKKLTLNIYNES"
BASE COUNT          182 a           81 c           52 g          191 t
ORIGIN      
        1 ttactttcat tataaatatt taatgttaat ttcttattta gataacaata aaaaaatata
       61 atctctaaaa acccaatgaa acagctgctc caaacaatta gttcttctaa ttgaaaatca
      121 aataaataag tatttcttct tacagtacca ccaagtaaca caatacccgc gaatatggtc
      181 catcctatcg atttattttt aactggtttg acaatattta atttttcata atcattctta
      241 gtgattttga catatgtttt cggtatgagc cagttaataa atggaaagaa gaagacaatc
      301 caattacttg ccaaatcaat cattaaatat tcactatcgt atttgattat tcgatattta
      361 gggtttttat taataacttt agattcgcaa agcaatgtct ccacatccct ttaattttat
      421 gtgtaataca tttttcgata cttcaaaaga cattcaaata ctatcaagtt actgtcatca
      481 aaggttttat taactgatat tttcat
//
LOCUS       DAMQYT010000433          504 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.444, whole genome shotgun sequence.
ACCESSION   DAMQYT010000433 DAMQYT010000000
VERSION     DAMQYT010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.444"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..>504
                     /locus_tag="PGA33_002910"
     CDS             <1..>504
                     /locus_tag="PGA33_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDG6474628.1"
                     /translation="KAIGVLLLIVGIVIMNQFNKNNLLLTDQKYLLFWLLLGFIFGFF
                     PPIQTTINSALASHTHSPAFASLVSFTIGSIALLILTAIFNRSLKLKTSHLKFGKLKP
                     IYFTGGILGMAFVTANIILMPHMGAALTTLIGMFGQILMGILIDHFGLFGSPKIAMTS
                     RKTIGLL"
BASE COUNT          155 a           84 c           78 g          187 t
ORIGIN      
        1 taaagcaatt ggagtattgt tactcattgt cggcatcgtc ataatgaatc aatttaacaa
       61 gaataattta ttactaactg atcaaaagta tttactgttt tggcttctat taggatttat
      121 ttttggtttc tttccaccta ttcaaacgac aattaatagt gctttagcta gtcatactca
      181 ttcaccagcc tttgcatcat tagtatcatt tacaattggg tcaatagcgc tattgatttt
      241 aaccgctatt tttaatcgtt ctttaaaact aaaaacaagt catttaaaat tcggtaaatt
      301 aaagcctatc tattttactg gcggtatact tggcatggct tttgtaacag ctaacattat
      361 cttaatgcct catatgggtg cagcattaac aacacttatt gggatgtttg gccagattct
      421 aatgggcata ttgatagatc actttggatt atttggttca cctaaaatag caatgacatc
      481 cagaaaaact attggtctat tatg
//
LOCUS       DAMQYT010000434          495 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.445, whole genome shotgun sequence.
ACCESSION   DAMQYT010000434 DAMQYT010000000
VERSION     DAMQYT010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.445"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            167..355
                     /locus_tag="PGA33_002911"
     CDS             167..355
                     /locus_tag="PGA33_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474629.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTSKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
BASE COUNT          152 a           70 c           63 g          210 t
ORIGIN      
        1 atttgtggtg tgatactcat cgtatccttt accattgcaa tgataactaa aaactggtta
       61 agtgaaatat tattttttat aaatatcgta ctcgttctct attacattta tcctattttt
      121 tatagtttta taggttaata attacggtat ataaggaggg tttcacatgt ctttacattt
      181 tgcaattctg ttttggctag cattaatttt cttagttgcc gctacgttta tactcgtatt
      241 aatgaaaaaa actagcaaag aatctaaaaa agagtcctat ttaagtttca ctgtcattct
      301 ctatattttt ggattcgcta tattaatata cacatttata tttggtgtgc tataaatgaa
      361 attcatttta tatccagtct gaaacgtctc aatgatgttt cagactgttt tttattctta
      421 atagacattt cacactctca tactttaata tttaagatat gttttagtct tacaagctta
      481 ttaaagatta caatt
//
LOCUS       DAMQYT010000435          466 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.446, whole genome shotgun sequence.
ACCESSION   DAMQYT010000435 DAMQYT010000000
VERSION     DAMQYT010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.446"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            81..>466
                     /locus_tag="PGA33_002912"
     CDS             81..>466
                     /locus_tag="PGA33_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HDG6474630.1"
                     /translation="MHAMQYHVKLPSDYNMEIIRDRVRLNGYKTDGFKNLIIKAYLIS
                     QTTTNCITNTYSPLYLWRSSEGMTEFIFNGFYDNVISSFGWQNINIGVIYSMNITDSV
                     KHSLYALEEYIDIFPTLSLKEIEIKK"
BASE COUNT          173 a           52 c           70 g          171 t
ORIGIN      
        1 ttatgataac tatatcacaa ttggataata tgagatttta cttattattg ctatcctttt
       61 tataaaaggg ggtatgctaa atgcatgcaa tgcaatatca tgttaagttg cctagtgatt
      121 acaatatgga aataattaga gatagagttc gattaaatgg ttataagact gatggattta
      181 aaaatttaat tataaaagct tatttaataa gtcaaacaac tactaattgc attacaaata
      241 cttattcacc attatattta tggagaagtt cagaggggat gactgaattt atattcaatg
      301 gattttatga taatgttatt tcttcttttg gatggcaaaa tataaatatt ggtgtgatat
      361 attcaatgaa cataaccgat tctgttaaac atagtcttta tgcgcttgaa gaatatatcg
      421 acattttccc tactttatct ctaaaagaaa tagaaatcaa aaaatt
//
LOCUS       DAMQYT010000436          465 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.447, whole genome shotgun sequence.
ACCESSION   DAMQYT010000436 DAMQYT010000000
VERSION     DAMQYT010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.447"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..195
                     /locus_tag="PGA33_002913"
     CDS             <1..195
                     /locus_tag="PGA33_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDG6474631.1"
                     /translation="NELLREKMSTGNLILSDERFDLKEIINFNQIIGKIHIENVYIET
                     RKGKVHYSKTGAHIVPYIDK"
     gene            206..400
                     /locus_tag="PGA33_002914"
     CDS             206..400
                     /locus_tag="PGA33_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474632.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGFVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
BASE COUNT          192 a           42 c           89 g          142 t
ORIGIN      
        1 aatgaattgt taagagaaaa aatgtcaaca ggcaatctaa tattaagtga tgagcgattt
       61 gatttgaaag agattattaa ttttaatcaa attattggaa aaatacatat cgaaaatgtg
      121 tatattgaaa ccagaaaggg aaaagtgcat tattcgaaga caggtgctca tatagtacct
      181 tatattgata agtaggtgaa aagtattgag aattgaagat gcatatcgta aagatgttat
      241 tatcacgctt ttgaataatg aagaatacga agggtttgta actgactatg aaaatgaatt
      301 cgagagcgaa acaggaaatc ttgttgtaga tatacagact gattttgctg tttattcgtt
      361 tgatgaaact gagataaaaa gtattagatt attaaaataa ttacaaaata gcatcctttc
      421 tacaaaaagc taaaaaagaa atgcttgaaa tgataagtaa tggta
//
LOCUS       DAMQYT010000437          460 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.448, whole genome shotgun sequence.
ACCESSION   DAMQYT010000437 DAMQYT010000000
VERSION     DAMQYT010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.448"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(30..422)
                     /locus_tag="PGA33_002915"
     CDS             complement(30..422)
                     /locus_tag="PGA33_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474633.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKENYNKYIQGQKQRVIFENGHFV"
BASE COUNT          155 a           86 c           37 g          182 t
ORIGIN      
        1 cctcttactc taattatatt cactatcaat tagacaaaat ggccattttc aaatatcacg
       61 cgttgtttct gaccttgaat atatttatta taattctctt tttgaaaatc agttaacatt
      121 aatttagatg taccgtattt taacactttt tgcattgttt ctattctcat ttttctaaat
      181 aaccatccat cttttaacac aatacgatta acagcatcat atgataattc tactgtttct
      241 ttaatttcaa atgtcttgaa tgaaataatc gtgcacatta aaaacgtatc accaaagtaa
      301 taaacatcta aatcatcacg tttatgttgt ccaactaaca aacgaccata ttcgaactct
      361 ttttctggat atttcaattc taaaaaacta ataatctctt cttcttttaa tttgaattgc
      421 atttaaaaac atcctctctt aagttttaac aagccttaat
//
LOCUS       DAMQYT010000438          452 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.451, whole genome shotgun sequence.
ACCESSION   DAMQYT010000438 DAMQYT010000000
VERSION     DAMQYT010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.451"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
BASE COUNT          164 a           61 c           49 g          178 t
ORIGIN      
        1 aaatagaagt ataaggtggt gaacaaaaat taacattgtg tataacatta tagtatatat
       61 agcatgcatg attaatttcg gattaattat agtcccatta ttaacaacaa atgatgcgga
      121 ttttgtatgg acttcttcat cattaatata tatgattctt cttacgtcga ttatcctaat
      181 agtttatatt aaaactaaag atagcttaca gttaacgata tttatactta atatagcctt
      241 aattattcta tactgtgcac cattactatt catttatgta ttctagtgct tcattaatat
      301 tttaatgcat cagtttaagt aaattccatt tggaaaataa aaagcaaata tcatcatcaa
      361 attaacatat ttaacgttat actttcataa aatttgaaac aattgaacat ccttacttta
      421 gctttatatc tttatttcaa gtcatgtttt ca
//
LOCUS       DAMQYT010000439          444 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.452, whole genome shotgun sequence.
ACCESSION   DAMQYT010000439 DAMQYT010000000
VERSION     DAMQYT010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.452"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..171)
                     /locus_tag="PGA33_002916"
     CDS             complement(<1..171)
                     /locus_tag="PGA33_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474634.1"
                     /translation="MNTIYEPSSICMIRTPLLSVEFFNLFLNTEQIKYSDLQLNAQMK
                     ESILTTTFNLYCT"
     gene            complement(236..379)
                     /locus_tag="PGA33_002917"
     CDS             complement(236..379)
                     /locus_tag="PGA33_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HDG6474635.1"
                     /translation="MEKVLDLDVQVKANNNSNDSAGDERITSHSLCTPGCAKTGSFNS
                     FCC"
BASE COUNT          146 a           74 c           64 g          160 t
ORIGIN      
        1 agtacaatat aagttaaatg ttgttgttag gattgattct ttcatctgag catttaattg
       61 taagtcgcta tatttaattt gttcagtatt taaaaataaa ttgaaaaatt caactgataa
      121 taaaggtgtt ctaatcatac aaatacttga cggttcgtaa atagtgttca tttgtaagca
      181 cctcacatat ttgaatagac aaatagagcc ctttcaggct ctatttgctg taatcttaac
      241 agcagaagct attaaaacta ccagtcttag cacaaccagg agtacataaa ctatgacttg
      301 taatacgttc gtcacctgct gaatcatttg agttattgtt tgctttaact tgcacgtcta
      361 aatcaagaac tttttccata gataaaacct cctattatta gttgaagtta aggcctactt
      421 caattgtcat attatccctt ttca
//
LOCUS       DAMQYT010000440          444 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.453, whole genome shotgun sequence.
ACCESSION   DAMQYT010000440 DAMQYT010000000
VERSION     DAMQYT010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.453"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
BASE COUNT          182 a           55 c           60 g          147 t
ORIGIN      
        1 tttcaaccga taagataaaa tgtcttgtcg gtttttaata taacaagtga tattttggaa
       61 atgccaaaaa tatatagatt gtttatataa aatgatttaa aatgttttat aacaacatat
      121 tttatgcaac acataaaatg ggtaactaac atcacgaaat cattgtattt gttgttacac
      181 tgatgaagcg atgtcacaaa gaaatgtcgt tatcaaacga agcccgaaat acatactgat
      241 actcgaaata atgtaacgat aaataacaat gataacgatt gattactacg caaaaatata
      301 tgtaaattga cgatttaaat gcaattcgta tattttgcca atgatttaca cattaaaatg
      361 tttgaaaatt gtcacaattc agtctaattt aaaaggaagt taattacaaa attaaaaaat
      421 catgcatcgt gtcatttaga tatt
//
LOCUS       DAMQYT010000441          442 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.454, whole genome shotgun sequence.
ACCESSION   DAMQYT010000441 DAMQYT010000000
VERSION     DAMQYT010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.454"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
BASE COUNT          139 a           73 c           66 g          164 t
ORIGIN      
        1 acttattaca agtacatcat atattagttg ataacgatta tcaatgtcgc gtggatttgt
       61 gacacatttc ttttaaaaat tcacaaggtt atggggcaga aatgataaag agccactaat
      121 gatttattat gtagtggttc tgggagtggg acagaaatga tattttcaca aaatttattt
      181 cgtcgtccca ccccaacttg cattgtctct agaaattggg aatccaattt ctctttgttg
      241 ggtccctgaa tatagccttg tagagtctag tacattgatt tgtatcccaa tgtccctata
      301 attgattatt cgctttatct aatgatccta tgactcaact attaaatcat ttttcgaaat
      361 acttaattct aatataatta aattcattta ttgtaatatt gcaaaaatac attgcacacc
      421 ttgttcatca atgctataat ta
//
LOCUS       DAMQYT010000442          428 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.455, whole genome shotgun sequence.
ACCESSION   DAMQYT010000442 DAMQYT010000000
VERSION     DAMQYT010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.455"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            70..417
                     /gene="sarR"
                     /locus_tag="PGA33_002918"
     CDS             70..417
                     /gene="sarR"
                     /locus_tag="PGA33_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HDG6474636.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
BASE COUNT          186 a           49 c           63 g          130 t
ORIGIN      
        1 agtttcgatt taataaatta catgtgaacc ttgctacaac aagatgtgca tcagaggagt
       61 ggtttaataa tgagtaaaat taatgacatt aatgatttag tcaacgcaac atttcaagtt
      121 aagaagtttt tcagagatac aaaaaagaag ttcaatttga actatgaaga aatttatatt
      181 ttaaatcata ttttaagaag tgagtctaac gaaatctcat ctaaagagat tgctaagtgc
      241 tcagagttca aaccttacta tttaactaaa gctttacaaa agctaaaaga tttaaaattg
      301 ttatcaaaga aaagaagttt acaagacgaa agaacagtta ttgtttatgt tacagataca
      361 caaaaagcaa atattcaaaa actgatttca gaattagaag aatacattaa aaattaaatc
      421 aaggttaa
//
LOCUS       DAMQYT010000443          425 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.456, whole genome shotgun sequence.
ACCESSION   DAMQYT010000443 DAMQYT010000000
VERSION     DAMQYT010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.456"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..218
                     /locus_tag="PGA33_002919"
     CDS             <1..218
                     /locus_tag="PGA33_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDG6474637.1"
                     /translation="EDLEPWTSCEFDFTRKGNLKVSFDYIDWIKLGFGPSGKENYYMY
                     KKFGILPDMEYEMEEIRAVEKYVKEQE"
BASE COUNT          172 a           45 c           78 g          130 t
ORIGIN      
        1 aagaagattt agaaccgtgg acatcatgtg aatttgactt tacaagaaag ggaaatttaa
       61 aagtatcatt tgattatata gattggatta aattaggttt tggcccatca ggaaaggaaa
      121 actactatat gtacaaaaaa tttggtattt taccagatat ggaatatgaa atggaagaaa
      181 ttcgagcagt agagaagtat gttaaagagc aagagtagca gacatgttat aaaagactgt
      241 gcaaaatcac cctcgtttta catttgattc aaagaagaag gtaaaagata agattatttg
      301 caacttaaaa agtcaattag cttatcggta tccatacatc atggataaat gagttcaact
      361 aattaacaaa tcacgatata aatttaaaat tgtcattaat tgaaaaggtt attgtagtgt
      421 atttt
//
LOCUS       DAMQYT010000444          416 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.457, whole genome shotgun sequence.
ACCESSION   DAMQYT010000444 DAMQYT010000000
VERSION     DAMQYT010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.457"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..>416
                     /locus_tag="PGA33_002920"
     CDS             <1..>416
                     /locus_tag="PGA33_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDG6474638.1"
                     /translation="KEAEVKKSFEKTLSMYPIKNLEDLYDKEGYRDDQFDKNDKGTWI
                     INSEMVIQPNNEDMVAKGMVLYMNRNTKTTNGYYYVDVTKDEDEGKPHDNEKRYPVKM
                     VDNKIIPTKEIKDEKIKKEIENFKFFVQYGDFKNLK"
BASE COUNT          187 a           46 c           77 g          106 t
ORIGIN      
        1 aaagaagcgg aagttaagaa aagctttgaa aaaacattga gtatgtaccc tattaaaaat
       61 ctagaggatt tatacgataa ggaaggctat cgtgatgatc agtttgataa aaatgataaa
      121 ggtacatgga ttataaattc tgaaatggtt attcaaccta ataatgaaga tatggtagct
      181 aaaggcatgg ttctatatat gaatagaaat accaaaacaa caaatggtta ctactatgtc
      241 gatgtgacta aggacgagga tgaaggaaaa ccgcacgaca atgaaaaaag atatccggtt
      301 aaaatggtcg ataataaaat cattccaaca aaagaaatta aagatgaaaa aataaaaaaa
      361 gaaatcgaaa actttaagtt ctttgttcaa tatggcgact ttaaaaattt gaaaaa
//
LOCUS       DAMQYT010000445          412 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.458, whole genome shotgun sequence.
ACCESSION   DAMQYT010000445 DAMQYT010000000
VERSION     DAMQYT010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.458"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..381)
                     /locus_tag="PGA33_002921"
     CDS             complement(<1..381)
                     /locus_tag="PGA33_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDG6474639.1"
                     /translation="MLLFYIIGVLVGMLIPIQTSVNSRLSLYTKSPFYTSFISFSVGT
                     ICLIILNIIINPEVFTIHFYNNQSFNYTWVVGGLLGVSFLTGNLLLLPKLGATLTVIA
                     TVAGQIIMGVIIDTFGLFGATIHDF"
BASE COUNT          169 a           62 c           65 g          116 t
ORIGIN      
        1 aaaatcatga attgtagcgc caaataatcc aaatgtatca ataatgacac ccataataat
       61 ttgacccgca actgttgcaa ttacagttaa tgttgcacct aattttggca ataacaataa
      121 attgccagtt aaaaagctaa ccccaagcaa accaccgact acccatgtgt agttaaatga
      181 ttgattattg taaaagtgaa tagtaaatac ttctggattg ataatgatat ttaaaataat
      241 taaacaaatt gttccaactg aaaatgaaat gaatgaagta tagaaaggcg atttagtata
      301 cagtgatagt cttgaattga cagatgtttg gataggaata agcattccaa ctaggacacc
      361 aatgatatag aaaagaagca tatgaataaa gtctcacttt ccaaagttaa ta
//
LOCUS       DAMQYT010000446          411 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.459, whole genome shotgun sequence.
ACCESSION   DAMQYT010000446 DAMQYT010000000
VERSION     DAMQYT010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.459"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
BASE COUNT          136 a           57 c           49 g          169 t
ORIGIN      
        1 ttcatttaat atcgagttta cagcaacaat tatgaacata tgtcgctaca ctctaattct
       61 aggatttata cttatgtcct tacctgatgt tgttgataaa aacatcaaga aaattatatt
      121 cgatgcattt attatcatcg tctttatctt ctttttacta tagtagtttt attttgtaat
      181 tacacagtta atcatatttg tttcgtatcg aattcctttg tgcattaaag ataattctaa
      241 tagaaaacag cagtaagact tttctaagtg ttagcatcct tactgctgtt tttatttgta
      301 acaacataat gacaggtact ttatatttca ctgtcaaaga ttattaatga ttaacttaat
      361 taaattatga tttgaataaa tagatatcta aatgcaattt gtcactattg a
//
LOCUS       DAMQYT010000447          405 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.460, whole genome shotgun sequence.
ACCESSION   DAMQYT010000447 DAMQYT010000000
VERSION     DAMQYT010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.460"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..>405
                     /locus_tag="PGA33_002922"
     CDS             <1..>405
                     /locus_tag="PGA33_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDG6474640.1"
                     /translation="LISGYFQLFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFR
                     QFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQ
                     IEDKAIKQTQFLQPILFLILGLFIVAIYLVIML"
BASE COUNT          149 a           45 c           61 g          150 t
ORIGIN      
        1 ctaatatcag gctatttcca attatttaaa acttattttg taactaatga attagtgttg
       61 ttttataaaa atggtattac acttcaatca atagtagacg tttatattaa ccatagtagt
      121 gatccattta gacagtttct aggtaaatac ttattaactt attcagaaat gggatatggt
      181 ttgcctcaaa ttttagaaaa actaaaatgc tttaagcctc aattaattaa gtttgtgcta
      241 caaggtgaaa agagagggaa gctagaagta gaactaaagt tatattcgca aatattagta
      301 aaacaaatag aagataaagc gataaaacag actcagtttt tacagcctat tttatttttg
      361 attttaggtt tatttattgt cgcaatttat ttagtaatta tgtta
//
LOCUS       DAMQYT010000448          400 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.461, whole genome shotgun sequence.
ACCESSION   DAMQYT010000448 DAMQYT010000000
VERSION     DAMQYT010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.461"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            60..>400
                     /locus_tag="PGA33_002923"
     CDS             60..>400
                     /locus_tag="PGA33_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDG6474641.1"
                     /translation="MKKFIGSVLATTLILGGCSTMENESKKDTKTETKSVPEEMEASK
                     YVGQGFQPPAEKNAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVH
                     CEDHGIVFNASL"
BASE COUNT          162 a           44 c           81 g          113 t
ORIGIN      
        1 ttataaaaat agggtaaaat atagatatat tgtattaatt aaattattcg aggtgtcata
       61 tgaaaaaatt cattggatca gttttagcta cgacattaat tttaggggga tgttccacga
      121 tggaaaatga atcaaaaaaa gacacaaaaa cagaaacaaa atctgtacca gaagaaatgg
      181 aagcttcaaa atatgtaggc caaggctttc aaccgcctgc agaaaaaaat gcgattgaat
      241 ttgcgaagaa gcatcgtaaa gaatttgaaa aagtaggtga acaattcttt aaagataact
      301 ttggactaaa agttaaagct acaaatgttg taggtaaaga tgatggtgta gaagtttatg
      361 tgcattgtga agatcatggc attgtattta atgcaagtct
//
LOCUS       DAMQYT010000449          394 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.462, whole genome shotgun sequence.
ACCESSION   DAMQYT010000449 DAMQYT010000000
VERSION     DAMQYT010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.462"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            68..>394
                     /locus_tag="PGA33_002924"
     CDS             68..>394
                     /locus_tag="PGA33_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HDG6474642.1"
                     /translation="MEILNDSVKSFEKLYLQLQDGFPVIVPTDTNYNLCSLPNNDLCI
                     DKIFEYKKRSKDKPLSLFIDKPEDWKLYGDNQNTEIVDKLVEIFWPGPLNIILKNKTS
                     YNYMLNN"
BASE COUNT          157 a           43 c           62 g          132 t
ORIGIN      
        1 ttttcacctg aagtagcacc tgaagataca gtggatttac ttcagataat aaaaaggagg
       61 agtaaagatg gaaattttaa atgattctgt aaaaagtttt gaaaaacttt atttacaatt
      121 acaagatggt tttccagtga ttgttcctac tgatactaat tataatttat gtagtttacc
      181 taacaacgat ctttgtatcg ataaaatatt tgaatataaa aagcgatcga aagataagcc
      241 attatcatta tttattgata agccagagga ttggaaattg tatggagata atcaaaatac
      301 ggaaatagtt gataaactag ttgaaatatt ttggcctgga ccattaaata ttattttaaa
      361 aaataaaaca agctataatt atatgctcaa taat
//
LOCUS       DAMQYT010000450          391 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.463, whole genome shotgun sequence.
ACCESSION   DAMQYT010000450 DAMQYT010000000
VERSION     DAMQYT010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.463"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            110..>391
                     /locus_tag="PGA33_002925"
     CDS             110..>391
                     /locus_tag="PGA33_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDG6474643.1"
                     /translation="MEILLCEVRVVNKNPRYRIIKYKNDYLMIDLVSTWLVLFFPFIN
                     WLIPKKYVQISREDFDNLNIVKPVKNKALWPAIGSILLFGTMFRDKIYIP"
BASE COUNT          154 a           45 c           65 g          127 t
ORIGIN      
        1 attgtagttc gtaagttaag taataaacag gaaaatagta tgttactaaa atacaaaaca
       61 cttataaagt atctgaaagt gtattagata taaaaccaac gagacggaag tggagatatt
      121 gctttgcgag gttagagttg taaacaaaaa tcccagatat agaattatta aatataaaaa
      181 cgactatctg atgattgatt tagtaagcac ctggttagtt ttgttctttc cgtttattaa
      241 ttggttaata ccaaaaaaat atgtccaaat cagcagagaa gattttgata atttaaatat
      301 tgtcaaacca gttaaaaata aagctttatg gccagctata ggtagcatac ttttatttgg
      361 cactatgttt agagataaaa tatatatacc t
//
LOCUS       DAMQYT010000451          371 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.464, whole genome shotgun sequence.
ACCESSION   DAMQYT010000451 DAMQYT010000000
VERSION     DAMQYT010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.464"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..286
                     /locus_tag="PGA33_002926"
     CDS             <1..286
                     /locus_tag="PGA33_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDG6474644.1"
                     /translation="NDKEFKEGMERARKEVNYAANTDAVATLFSTKKNFTKDNTVDDV
                     IELSDKLYNLKNKPDKSTITIQIGKPTINTKKAFYDDNRPIEYGVHSKDE"
BASE COUNT          170 a           40 c           61 g          100 t
ORIGIN      
        1 aaatgataaa gaatttaaag aaggaatgga acgagcaaga aaagaagtga attacgctgc
       61 aaatacagat gctgttgcta cacttttttc tactaagaaa aactttacta aagacaatac
      121 agtagatgat gtaatcgaac taagtgataa attatataat ttaaaaaata aaccagataa
      181 atctacaatc acaatacaaa tagggaaacc cactattaat actaagaaag ccttttatga
      241 tgataatcgt ccaatagaat atggggtgca cagtaaagat gaataaaatt aatgataggg
      301 atttaacaga attaagtagt tactgggttt atcaaaatat tgatataaaa aaagaattta
      361 aagttaatgg a
//
LOCUS       DAMQYT010000452          363 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.465, whole genome shotgun sequence.
ACCESSION   DAMQYT010000452 DAMQYT010000000
VERSION     DAMQYT010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.465"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..315)
                     /gene="sarU"
                     /locus_tag="PGA33_002927"
     CDS             complement(<1..315)
                     /gene="sarU"
                     /locus_tag="PGA33_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HDG6474645.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRK
                     KIA"
BASE COUNT          109 a           54 c           46 g          154 t
ORIGIN      
        1 agcaatcttt ttacgttgta ttggagtaac tgaaacaaaa atacggcgtt catctgcttc
       61 gtttctagac ttatttataa atcctttttt agatagtgac ttaatgttct ttacaacatc
      121 agattgtttg taaagtatgt caccaatgat ttcttttagt gaaatactgt gttcattttt
      181 gtaataaaaa tatgctaata tcaacaattc ttttaatgat aatttatatt gttgctttaa
      241 ctcttgagtg agaaaaaata tgtacgcttc tacatttatg aatttattga ccttttcgaa
      301 agtttgatag tccaccttac attctcctac tattttctga tgtcccaata ctttatatta
      361 ttt
//
LOCUS       DAMQYT010000453          359 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.466, whole genome shotgun sequence.
ACCESSION   DAMQYT010000453 DAMQYT010000000
VERSION     DAMQYT010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.466"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            127..209
                     /locus_tag="PGA33_002928"
     tRNA            127..209
                     /locus_tag="PGA33_002928"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:160..162,aa:Leu,seq:gag)
     gene            232..305
                     /locus_tag="PGA33_002929"
     tRNA            232..305
                     /locus_tag="PGA33_002929"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:264..266,aa:Gly,seq:tcc)
BASE COUNT          124 a           63 c           70 g          102 t
ORIGIN      
        1 gttaacaact gataacacaa attaatttaa tgtaaaaaaa cgattaaaag ttattgacaa
       61 atatttttaa acataatata ataaataacg ttctgaaaaa cgaagatgtt ttaaatgcct
      121 atatccgcgg tcgtggcgga atggcagacg cgctaggttg agggcctagt gggagaaatc
      181 ccgtggaggt tcaagtcctc tcggccgcat caaaattctt aatttaaata agcgggtgta
      241 gtttaatggc aaaacctcag ccttccaagc tgatgttgtg ggttcgattc ccatcacccg
      301 ctccatagat aattttaatg aacattgaaa actgaatgac aatatgtcaa cgttaattc
//
LOCUS       DAMQYT010000454          339 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.468, whole genome shotgun sequence.
ACCESSION   DAMQYT010000454 DAMQYT010000000
VERSION     DAMQYT010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.468"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
BASE COUNT          132 a           42 c           33 g          132 t
ORIGIN      
        1 atagttttaa attttttatt aagtagaaaa caattattac agttgatttc attactgcaa
       61 accacatata aatttgtcga ttttactaca taacatagat tatcatagat tcttgaattt
      121 ttagcaaaat aactgttatt ttcattatat ttttacaaaa aaggttcgtt ttatatttta
      181 tgcatattac tgtaacagaa tcattaagat atgctattcg aatatacttt ttcaaaattt
      241 atataatgaa taaattaaca tgtattgaaa aaaaagcgaa atgcagccta tcctctaatg
      301 taaaccaaac gatatatctc gtcagacttt atatttaaa
//
LOCUS       DAMQYT010000455          319 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.469, whole genome shotgun sequence.
ACCESSION   DAMQYT010000455 DAMQYT010000000
VERSION     DAMQYT010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.469"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
BASE COUNT          135 a           34 c           47 g          103 t
ORIGIN      
        1 tttaaaggta ggtaaaacta aaataaaaga agctaaattt aaaacgataa tcaagttata
       61 tgaatatcaa agaacattag aaaataaaac agaatgttaa caaaaatatt ttgttaggtt
      121 aggtggacaa gaaattaaat ttctaatgat ttcatggttg tctgtccacg ttttgtccac
      181 ctcaataaga taatctctta gttttagaaa ttgtacatgg ttaaataatc aaaaatgcat
      241 attatgaaat tatcattgcc attaaatctt gaatgatctt taagcgatga tataaaaggc
      301 tgaacacagt aaataagtt
//
LOCUS       DAMQYT010000456          304 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.471, whole genome shotgun sequence.
ACCESSION   DAMQYT010000456 DAMQYT010000000
VERSION     DAMQYT010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.471"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            1..304
                     /locus_tag="PGA33_002930"
                     /pseudo
     CDS             1..304
                     /locus_tag="PGA33_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1829 domain-containing protein"
BASE COUNT          130 a           39 c           43 g           92 t
ORIGIN      
        1 ttctaatcct aagtcacaga acatatttac agaagatgtt gcaaacaaat tggatgaaca
       61 taacatttat tacggaagag atttacctat tataggaagc agtggtgttg ttcataattt
      121 cgactttttt attagcgcta agaaaaatca aaaagaaaaa tttatcaatg ctatttctaa
      181 ccctaataat tctatgatta ttaagtcgaa aataacggat gctatgcaag caaaaaaata
      241 aaaagacaca ggcaaaatga gtttattttt attttaaatg actcaaaaaa agaaataaat
      301 gaac
//
LOCUS       DAMQYT010000457          295 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.473, whole genome shotgun sequence.
ACCESSION   DAMQYT010000457 DAMQYT010000000
VERSION     DAMQYT010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.473"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
BASE COUNT           93 a           36 c           43 g          123 t
ORIGIN      
        1 gcttttttat atcaccactt attaaaaaat gtaagaatat tcctcagtgc taagtgaagg
       61 aggttctagt tttatttaat attcatattg ataggtaatg gttaataatt gcctgtaaat
      121 tattgttaaa tatcgttgtt atagtatcat cattcataaa atttttatag attttatcaa
      181 gtatttcctc atcttcaata gctgtaaagc gatgtactag tctttttatg agctatatat
      241 aattttgcga agctaaactg tttgtaatta ttcaccaatc aggttatgta gtgtc
//
LOCUS       DAMQYT010000458          294 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.474, whole genome shotgun sequence.
ACCESSION   DAMQYT010000458 DAMQYT010000000
VERSION     DAMQYT010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.474"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
BASE COUNT          135 a           25 c           46 g           88 t
ORIGIN      
        1 ggagttagta gtttgaatat tgaaagacac taatctaata aaaatacata aaaggttaaa
       61 ctttagaagt ggctgtaaac ttcaatgaat actaaacatt gaagttttgt aaaaggcgta
      121 aggtatacaa gacaaatcgt ttatctttga caaagtaatt gatgatgaaa agtcgttaat
      181 atgtagaaac aaaaattgaa aatataaaat ttaactatta tgaagtgatg atagccatta
      241 atgaatagaa aaataataaa atcatttata gataaaaaat acgttaagtt caca
//
LOCUS       DAMQYT010000459          290 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.475, whole genome shotgun sequence.
ACCESSION   DAMQYT010000459 DAMQYT010000000
VERSION     DAMQYT010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.475"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
BASE COUNT           85 a           44 c           78 g           83 t
ORIGIN      
        1 ggtcggccta aaatttacgc tgacctttgt ctattgtgta tgagcttgag tgaagcagga
       61 catggcagaa tgctacaagt gaatttggaa gattgtattt atattggcga tattgatgtt
      121 taacatatag tattagtgat ttaacatgac cgtgtagctt ttagcgtatg caggcgcaga
      181 aatgcaagca cggagtgcgt taagcatttg ggcggagcct gcgacaaata cgcgttaaaa
      241 aagcgagaac ggtcatgtat caaatcgctt gcgatttgat aggaggcata
//
LOCUS       DAMQYT010000460          286 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.477, whole genome shotgun sequence.
ACCESSION   DAMQYT010000460 DAMQYT010000000
VERSION     DAMQYT010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.477"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..>286
                     /locus_tag="PGA33_002931"
     CDS             <1..>286
                     /locus_tag="PGA33_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474646.1"
                     /translation="NLYDVYISKGLDTPKKIGPKYAPVGASNDPNNMNARWIPTVEKI
                     MKDLGGSEAKTSCSNGKGKSIKFNGKLPHWSNDDPGKGNLYTAGQCTWYAY"
BASE COUNT          100 a           41 c           59 g           86 t
ORIGIN      
        1 aatttgtatg atgtttatat ttctaaaggt ttagatactc ctaaaaaaat aggtcccaaa
       61 tatgcgcctg ttggtgcgtc aaatgaccct aataatatga atgcccgttg gataccaaca
      121 gttgaaaaaa ttatgaaaga tttaggcggt tcagaagcta aaacgtcatg tagtaatggt
      181 aaaggaaagt ctataaagtt taatggtaaa ttgccacatt ggtcaaatga tgaccctgga
      241 aaaggtaatt tatacactgc tggtcaatgt acttggtatg catacg
//
LOCUS       DAMQYT010000461          280 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.479, whole genome shotgun sequence.
ACCESSION   DAMQYT010000461 DAMQYT010000000
VERSION     DAMQYT010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.479"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
BASE COUNT          119 a           29 c           43 g           89 t
ORIGIN      
        1 gagatacata gcaatgtaaa ggtatcaaat agttatttca taatttatta tgatgacata
       61 attttcagta gatagttgta aattaacact gaaatagatg tttatattaa gaagacttta
      121 atatcataat taatcaaaat cttgcgtcaa acatttaaac gaattttaag ttgataacaa
      181 ttggcaaaac attgttcaaa catcacaatg ataaagcata ttatcagtat tgtagtgtgt
      241 ggaaaatgac agccatctaa ggagaaaaat gatgaaaaga
//
LOCUS       DAMQYT010000462          279 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.480, whole genome shotgun sequence.
ACCESSION   DAMQYT010000462 DAMQYT010000000
VERSION     DAMQYT010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.480"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            178..>279
                     /locus_tag="PGA33_002932"
     CDS             178..>279
                     /locus_tag="PGA33_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HDG6474647.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKK"
BASE COUNT          116 a           31 c           40 g           92 t
ORIGIN      
        1 atattagtaa ttatagtttc attaatttat tgcaacaata cataggatca ttattacaag
       61 aattatttaa tatcatctta gaatctcaca aaatatcaac tttgtttaat tactcaaaat
      121 gataagtcat ttagagtttt taaaataaat ttttgcgaaa taaaggagac atgttaaatg
      181 aaaaaagtaa tggggatatt attagcaagt acacttatct taggtgcttg tggacatcat
      241 caagatagtg caaaaaaaga gagcactagt cacaaaaag
//
LOCUS       DAMQYT010000463          279 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.481, whole genome shotgun sequence.
ACCESSION   DAMQYT010000463 DAMQYT010000000
VERSION     DAMQYT010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.481"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            74..>279
                     /locus_tag="PGA33_002933"
     CDS             74..>279
                     /locus_tag="PGA33_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDG6474648.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFF"
BASE COUNT           97 a           35 c           49 g           98 t
ORIGIN      
        1 tacttttttt attagcaggt gtaagctata atataaagag ttgtctcatg gacgattgat
       61 tggaggaacg aaaatgcaca aaaaatattt tattggcact tccattttaa tagcagtatt
      121 tgtcgttata tttgaccaag ttactaaata tattatagct actacaatga aaattggaga
      181 ttcatttgaa gtgataccgc actttttaaa cataacatca catcgaaata atggtgctgc
      241 atggggaata ttgagtggaa aaatgacatt tttctttat
//
LOCUS       DAMQYT010000464          278 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.482, whole genome shotgun sequence.
ACCESSION   DAMQYT010000464 DAMQYT010000000
VERSION     DAMQYT010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.482"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..236)
                     /locus_tag="PGA33_002934"
     CDS             complement(<1..236)
                     /locus_tag="PGA33_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDG6474649.1"
                     /translation="MKPFESHNKQLKILRRRGMEVPSSAKRDLENENYYNIINGYKDL
                     FLELDVNGNFLVPDKYKQGTHFKEVFSLYKLDRK"
BASE COUNT           96 a           48 c           24 g          110 t
ORIGIN      
        1 aattttctat ctagtttgta taaagaaaag acttctttaa aatgagtacc ttgcttatat
       61 ttatcaggaa ccaaaaaatt accattaaca tctagttcta aaaataaatc tttataacca
      121 tttatgatat tataataatt ttcattttct aaatctcttt tagcactact cggtacttcc
      181 attcctcttc ttcttagaat tttcaattgt ttattatgac tttcaaatgg cttcataaat
      241 ttacccctat aaaagtagcc ataacccgaa tagagtta
//
LOCUS       DAMQYT010000465          268 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.484, whole genome shotgun sequence.
ACCESSION   DAMQYT010000465 DAMQYT010000000
VERSION     DAMQYT010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.484"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
BASE COUNT          110 a           28 c           41 g           89 t
ORIGIN      
        1 tgatgaatga aagttgcctt tttatttttg gtaaaagtta atgcgtcagt gaattgtgta
       61 agtttttcaa aaagtaaaaa gaaataataa aggtgaatta ttagaattcc aaaaataatt
      121 cattacattc ataaagcatt ttacaaatgg taagaaaatg agtgttacaa atctaaatat
      181 tgcaaaagaa gctgatttag tcacaaaaaa tgtcctatgt aataattcga gaaagatgca
      241 ctatatacgg tcttcttact attcaaat
//
LOCUS       DAMQYT010000466          266 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.485, whole genome shotgun sequence.
ACCESSION   DAMQYT010000466 DAMQYT010000000
VERSION     DAMQYT010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.485"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..166)
                     /locus_tag="PGA33_002935"
     CDS             complement(<1..166)
                     /locus_tag="PGA33_002935"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="catalyzes the formation of 4-phospho-L-aspartate
                     from L-aspartate and ATP; lysine and threonine sensitive;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDG6474650.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRL"
BASE COUNT           94 a           39 c           38 g           95 t
ORIGIN      
        1 ataatctgat taacaaatca gttgttttaa tatcattatc atgtctttta cctggagcag
       61 aaacgataac aatctttcgc tctggatcag aattaacaat atttaaaacc tttttaattt
      121 gagtagcatt ggagacggag ctaccaccga atttggaaac tttcatagtg catgccaacc
      181 tttctaaaaa atcagaaaat tgtattcagt tttgtaagta agaatgatat tatgtattta
      241 tattttgaat attacgaact ttacaa
//
LOCUS       DAMQYT010000467          260 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.486, whole genome shotgun sequence.
ACCESSION   DAMQYT010000467 DAMQYT010000000
VERSION     DAMQYT010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.486"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..>260
                     /locus_tag="PGA33_002936"
     CDS             <1..>260
                     /locus_tag="PGA33_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDG6474651.1"
                     /translation="PQKVITDQAPSTKVAMVKVIKAFKLKPDCHCTSKYLNNLIEQDH
                     RHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFS"
BASE COUNT          100 a           47 c           43 g           70 t
ORIGIN      
        1 cctcaaaagg taattacaga tcaggcacct tcaacgaagg tagcaatggt taaagtaatt
       61 aaagctttta aacttaaacc tgactgccat tgtacatcga aatatctgaa taacctcatt
      121 gagcaagatc accgtcatat taaagtaaga aagacaaggt atcaaagtat caatacagca
      181 aagaatactt taaaaggtat tgagtgtatt tacgctctat ataaaaagaa ccgcaggtct
      241 cttcagatct acggattttc
//
LOCUS       DAMQYT010000468          255 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.488, whole genome shotgun sequence.
ACCESSION   DAMQYT010000468 DAMQYT010000000
VERSION     DAMQYT010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.488"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..223
                     /locus_tag="PGA33_002937"
     CDS             <1..223
                     /locus_tag="PGA33_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDG6474652.1"
                     /translation="KDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKVIDYCP
                     IDQIKLSLPGVNIHKHKLEVYGVCESCQD"
BASE COUNT           96 a           31 c           46 g           82 t
ORIGIN      
        1 taaagattta ggaattattg aaaatacaga acttgatggt gaaatgaagt ttagaatcgc
       61 ttgtacaaac catcatcatc atcattttat ctgtgaaaag tgtggagata caaaggtaat
      121 agattattgt ccaatagatc agataaaatt atcactacct ggtgttaata ttcacaaaca
      181 caaacttgaa gtttatggtg tatgtgagtc ttgccaagat taatataaag aaatgagatt
      241 tatgcacatt tggtc
//
LOCUS       DAMQYT010000469          255 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.489, whole genome shotgun sequence.
ACCESSION   DAMQYT010000469 DAMQYT010000000
VERSION     DAMQYT010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.489"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..>255)
                     /locus_tag="PGA33_002938"
     CDS             complement(<1..>255)
                     /locus_tag="PGA33_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6474653.1"
                     /translation="FKLFKKKSTFIAPIVFILLMVAQGYIATKYNEIFTPQESFTSAY
                     NGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTM"
BASE COUNT           98 a           31 c           40 g           86 t
ORIGIN      
        1 tcatagttgt tcttgaatat tcacgataga gtaaattttt aatcgtaccg taatgaaatt
       61 ccattgaaat gattgtactt gcttgaataa ttaataaaaa tgcaaaccat gaaaaaccat
      121 tataagcaga tgtgaaagat tcctgtggcg taaaaatttc attgtatttt gtagcaatat
      181 aaccttgagc aaccattagt agaataaaga caataggtgc gataaaagtt gatttctttt
      241 taaataattt gaaac
//
LOCUS       DAMQYT010000470          249 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.491, whole genome shotgun sequence.
ACCESSION   DAMQYT010000470 DAMQYT010000000
VERSION     DAMQYT010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.491"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..156
                     /locus_tag="PGA33_002939"
     CDS             <1..156
                     /locus_tag="PGA33_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDG6474654.1"
                     /translation="LSTMALSMLVTSSPQNIIEFLALIGMTACFFLLNMSSVLDKKIH
                     VILKTNK"
BASE COUNT           88 a           34 c           41 g           86 t
ORIGIN      
        1 ttgtcaacaa tggctctgag tatgttagta acttcatccc ctcaaaatat aatagaattt
       61 cttgctttaa ttggcatgac tgcatgcttc tttctactga atatgtcatc ggttctagat
      121 aaaaaaattc atgttatttt aaaaacaaat aagtagtaaa attgattaac ttaggtagta
      181 tcggatactt aaatgttggt tcataaaaag caatgatttt aaatcgagga gctatcttag
      241 aacagggaa
//
LOCUS       DAMQYT010000471          244 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.493, whole genome shotgun sequence.
ACCESSION   DAMQYT010000471 DAMQYT010000000
VERSION     DAMQYT010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.493"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
BASE COUNT          105 a           26 c           42 g           71 t
ORIGIN      
        1 gaaaaatata ggctattata ctttagaatt acaaattata aatcttgcca gtggatatgg
       61 gagttcaaca tgtcacttac cccaatttgt ggaatgttaa ataactataa agaggaaagg
      121 agaaaaaatg ataagaatgg ttaaagaaaa agaacgaata gcaaaattaa atttcgaata
      181 gctgtgacga gcttaataat tcaagtactt aattttatca aatcgttctt ttaaaaaggt
      241 aaag
//
LOCUS       DAMQYT010000472          244 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.494, whole genome shotgun sequence.
ACCESSION   DAMQYT010000472 DAMQYT010000000
VERSION     DAMQYT010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.494"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
BASE COUNT          100 a           24 c           33 g           87 t
ORIGIN      
        1 ttcattgaga atgcttttaa gtcacatgcc tattttttgt tctaatgcac caatagtaag
       61 cctttgatat tactgttgtg agcaatgtta ttaattaaaa taagatgtaa tagcgaattg
      121 aaaacaagct tagattaaaa gtgagtttta aaaatataga taaataattt aaagcaatta
      181 aagaaaaaaa gtattaaaaa ttgcaattct taaactgtga ttcatattaa tatttctagc
      241 aaat
//
LOCUS       DAMQYT010000473          236 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.496, whole genome shotgun sequence.
ACCESSION   DAMQYT010000473 DAMQYT010000000
VERSION     DAMQYT010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.496"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..>236
                     /locus_tag="PGA33_002940"
     CDS             <1..>236
                     /locus_tag="PGA33_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HDG6474655.1"
                     /translation="RFISYINSPIAITSANISGTADDILITENEAIKHMGENVRYMLR
                     SQNKTNYKTSSTIIKVTDNKIELLREGDIKFEEI"
BASE COUNT          107 a           25 c           36 g           68 t
ORIGIN      
        1 gaagatttat ttcatatatt aattcaccta tagcaattac ttcagcaaat atatctggaa
       61 ctgccgatga tattttaata actgaaaatg aagcaattaa gcacatgggc gaaaatgtaa
      121 gatatatgct aagaagtcaa aataaaacta actataaaac atctagtacg attattaaag
      181 tgacagataa taaaattgaa ttattaagag aaggagatat aaagtttgaa gaaata
//
LOCUS       DAMQYT010000474          236 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.497, whole genome shotgun sequence.
ACCESSION   DAMQYT010000474 DAMQYT010000000
VERSION     DAMQYT010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.497"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..>236
                     /locus_tag="PGA33_002941"
     CDS             <1..>236
                     /locus_tag="PGA33_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDG6474656.1"
                     /translation="LEGIFKTLLNKNSQVVFFIMPSILKSKNTMSEEEKFTIIKTIIE
                     SDLKIAFNINNIETTYFVEEAFMSVFRQISPNEL"
BASE COUNT           99 a           32 c           30 g           75 t
ORIGIN      
        1 tcttagaagg aatctttaaa accttattga ataagaacag tcaagttgtc tttttcatca
       61 tgccatcgat tctaaaaagt aaaaatacca tgtccgaaga agaaaaattc acaatcatta
      121 aaacaataat tgaaagtgat ctaaagatag catttaatat aaataatatt gaaacaactt
      181 attttgttga agaagctttt atgagtgttt tcagacaaat atctccaaac gaatta
//
LOCUS       DAMQYT010000475          216 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.502, whole genome shotgun sequence.
ACCESSION   DAMQYT010000475 DAMQYT010000000
VERSION     DAMQYT010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.502"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..141
                     /locus_tag="PGA33_002942"
     CDS             <1..141
                     /locus_tag="PGA33_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDG6474657.1"
                     /translation="FYLLVFENVQNLILYVSWLFMTMLFMFMNMHSIIDKKVHIFLKS
                     NK"
BASE COUNT           73 a           19 c           32 g           92 t
ORIGIN      
        1 ttttacttgt tggtatttga aaatgtgcaa aatttaatct tatatgtttc ttggcttttc
       61 atgactatgc tatttatgtt tatgaatatg cattcaatta tagataaaaa agtacatata
      121 ttcttaaagt ctaataaata gttacaaatt tagttagttt tcaattgtta attaggggtg
      181 gtaaacagtg ctttgtgaat ctagacaaat ttataa
//
LOCUS       DAMQYT010000476          212 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.503, whole genome shotgun sequence.
ACCESSION   DAMQYT010000476 DAMQYT010000000
VERSION     DAMQYT010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.503"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..>212
                     /locus_tag="PGA33_002943"
     CDS             <1..>212
                     /locus_tag="PGA33_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474658.1"
                     /translation="EIFKNIDHDFRDGVSEEMMKHLKVVKQNIDQIKNQNDIYGRQIA
                     DIRSIMKQQDATILDGNFQINCSGEN"
BASE COUNT           97 a           20 c           38 g           57 t
ORIGIN      
        1 gaaattttca aaaatataga tcacgacttc agagatggag tatctgaaga aatgatgaaa
       61 catttgaaag tagtgaaaca gaatatagac caaataaaaa atcaaaatga tatttatggt
      121 aggcaaattg cagatattag aagtattatg aaacaacaag atgcaacaat tttagatgga
      181 aattttcaaa ttaattgtag cggcgaaaat at
//
LOCUS       DAMQYT010000477          204 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.504, whole genome shotgun sequence.
ACCESSION   DAMQYT010000477 DAMQYT010000000
VERSION     DAMQYT010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.504"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(<1..>204)
                     /locus_tag="PGA33_002944"
     CDS             complement(<1..>204)
                     /locus_tag="PGA33_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDG6474659.1"
                     /translation="LFFPFINWFIPKRYVKISREEFENLNIVKPAKKNVFWPVAGIST
                     LFAVTLRKYTHLLDTQLDRKLVI"
BASE COUNT           76 a           31 c           28 g           69 t
ORIGIN      
        1 caataactaa ttttctatca agttgagtgt caagtaaatg tgtatacttt cttaatgtaa
       61 ctgcgaataa agtagagata cctgcaactg gccaaaaaac attcttttta gcaggtttca
      121 caatatttaa attttcaaac tcttctctac taattttgac gtatcttttg gggatgaacc
      181 aattaataaa aggaaaaaat aaac
//
LOCUS       DAMQYT010000478          204 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.denovo.505, whole genome shotgun sequence.
ACCESSION   DAMQYT010000478 DAMQYT010000000
VERSION     DAMQYT010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.denovo.505"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..>204
                     /locus_tag="PGA33_002945"
     CDS             <1..>204
                     /locus_tag="PGA33_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HDG6474660.1"
                     /translation="VKVEDLSIEETNRFYKLFQMAEEKHGFHFMNEDYFKRMQEIYKD
                     KAMLKIACINLNEYQDKLKIQLL"
BASE COUNT           90 a           15 c           31 g           68 t
ORIGIN      
        1 tgttaaggtt gaagatttat ctattgaaga aacaaatcga ttttataaat tgtttcaaat
       61 ggctgaagaa aaacatggtt ttcatttcat gaatgaagat tattttaaac gaatgcaaga
      121 aatatataaa gataaggcaa tgttaaagat agcttgtata aatcttaatg aatatcaaga
      181 taaattaaaa atacaattat tgaa
//
LOCUS       DAMQYT010000479         6868 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.guided.135, whole genome shotgun sequence.
ACCESSION   DAMQYT010000479 DAMQYT010000000
VERSION     DAMQYT010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6868
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.guided.135"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            245..430
                     /locus_tag="PGA33_002946"
     CDS             245..430
                     /locus_tag="PGA33_002946"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474661.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
     gene            complement(653..1231)
                     /locus_tag="PGA33_002947"
     CDS             complement(653..1231)
                     /locus_tag="PGA33_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDG6474662.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(1495..1875)
                     /gene="blaI"
                     /locus_tag="PGA33_002948"
     CDS             complement(1495..1875)
                     /gene="blaI"
                     /locus_tag="PGA33_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HDG6474663.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(1865..3622)
                     /gene="blaR1"
                     /locus_tag="PGA33_002949"
     CDS             complement(1865..3622)
                     /gene="blaR1"
                     /locus_tag="PGA33_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HDG6474664.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTML
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSN
                     KNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNDQ"
     gene            3729..4574
                     /gene="blaZ"
                     /locus_tag="PGA33_002950"
     CDS             3729..4574
                     /gene="blaZ"
                     /locus_tag="PGA33_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HDG6474665.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            complement(5100..5519)
                     /locus_tag="PGA33_002951"
     CDS             complement(5100..5519)
                     /locus_tag="PGA33_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDG6474666.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            5643..6614
                     /locus_tag="PGA33_002952"
     CDS             5643..6614
                     /locus_tag="PGA33_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDG6474667.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            complement(6786..>6868)
                     /locus_tag="PGA33_002953"
     CDS             complement(6786..>6868)
                     /locus_tag="PGA33_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDG6474668.1"
                     /translation="FVHIIAPVISGCAVAYYTYWLSKRNK"
BASE COUNT         2400 a          945 c          950 g         2573 t
ORIGIN      
        1 gttaaaatta tactataaat tcaactttgc aacagaacct catttactat atgggcatct
       61 ctttcattat taatacaaag ctttgtatct ccgatattag gcaaaggtgt aaacgaatta
      121 ggtgatacta taggattcaa cattatatta atcgtttctt taataatgat tatcttctta
      181 ttttttataa atcgtagtaa aaatatctcc tatgattata taaaagttca aagaggtgaa
      241 gccaatgaat aaattagaaa aatggtcttt ggtatttatt atttttacat ttctctcttc
      301 attagcactt ttatatttca aatataattt tagttcggtt atgccagtcc taagtctttc
      361 tattttaact atactaatat tgttgacgag tattattact gtagtttcag ttaatgatga
      421 agatacttaa aacgttcatt agtgaaacag gtattatatt aaaaaagcgt acgaatttgt
      481 acgctttttt cttgtgttat tattaaaatc tcataacagg aaaatattat gagattttag
      541 acgctgtcag aacgtctctt ttacatcgag tgacattgaa tcaaagtctt tgtatgttaa
      601 tgatatataa aattatttag tagattcatt atagtaagct agattagttg ctctacttgt
      661 ttttattctc ttttgcctct aatttattga gataacgata aatagttgta cgtgaaactt
      721 gccactgctc tgcaattgtt ttaattggcg taccattatc ataaagtgtc ttaagtaagt
      781 ttaaatcttg tttatttaat ttttcaggtc tacctccata tcggcctcta gcacgtgctg
      841 caattcgacc ggctgatgat cgctctaaaa ttaaattacg ttcaaactct gcaaatgctg
      901 cgaataaatg aaataataat tgtcctgtag aacttgattt atccatcgtt atattttctt
      961 ctaaactatg aaaactcaca cctcgttcgt taagttcatt aactaacgtg attaaatcct
     1021 ccatgttccg tcctagtcta tctaatctcc aaacaacaat cgtatctcct gacctcgcaa
     1081 attcaattgc tttatctaaa ccaggtcgct tactttttga accactcata tggtcattaa
     1141 atatcttttc acaaccatat gtatttaatc gatcctcctg taaattcaaa ttttgtaatc
     1201 cagttgaaac tcttgcataa cctattttca aaatacagac ctcctatata cacctttagt
     1261 gtaccataac ttgattacga ggtcttttgt gaacatagat tttttgttgg gtttttgaac
     1321 acataattct atgtaatact tacttattta gacaaaaaac aagagtgtac agaaaatggt
     1381 tattttctgg acactctcat ctttaataat atattttaaa tgtcattaac aaaactaatt
     1441 taataagagt caagtatggt ttacatatgt attatgtttg aaacatacct ctaattactt
     1501 tttactaata tcatttaaaa tgtctcgcaa ttcttcaatt tctttgttat ttaattcttc
     1561 atttttcgca aaattcagca ctaaactttt catatcccct ccatacagtt tattaagaaa
     1621 ggttttagca gttttcattt taatatcgtc ttctttaata tttgatgagt aaaaataaat
     1681 attttctgat ttgtatcgtt ttataatctc ttttttatat agtcttgtga ttaatgttct
     1741 aatcgtttta tcactaactt ctttatattt ttgaatttca actacaattt cattagctga
     1801 tactgatttt ttatcccata ttatattcat aacatcccat tcagccatag atatttcaac
     1861 ttgcttattg gtcatttaaa acacccattc cttttaatat tttttcactt attaattcag
     1921 catttttccc agacgggttt ccatctgata aatgtgtagc aaaataatac ttatcatgat
     1981 ttgtaattac gtaacctaca aaccacccat tattatactt cccgtttact atacctgtac
     2041 ctgttttccc atacagttca tacttttcat ttttcttaat caataatgaa gaagataatt
     2101 gattctttgc ttttttacta aaatggttat tttgttccat catattttta aaaactatta
     2161 cttgttcaag attagatatt ttcaaactat cttccatcca atagctttta taacttccca
     2221 aattttcatt accataattt aattgcttga gttgagccgc agtatagttc tttggtattt
     2281 gattactaat acgttcgaag taccaattaa ctgaattttg cattgctgta tttaaatctt
     2341 gttccttatt ccaagactcg aaaagataat gtttatgatt ccatgacata cgtgaatttt
     2401 tatcacttat aatatgacgg tcaagtccaa acagtgctaa ataaattttg taagttgaat
     2461 caggagaata tctttttcta ctttcttttt cattataaat atagtatttg tctttcttca
     2521 tgctgtacat aacgaaagat ccactatttg aaccaaaatt tttactttca tctaaaattt
     2581 ggtaatcgct ttgtaagggc tttttataat tataatcagt taaggattgc cccattaaaa
     2641 actggctttg aattatcatg ataaaaaaag taaaaataca aataaaaatt agaattaatt
     2701 ttgactgctt tttcagatcg gcttctttta tattaattaa tctacttttg agtaatgatt
     2761 ttttaccatt aaatgaatgg cttaatatat ttttgttgga aaatggggtt tttaatacgg
     2821 agtccataat agattccgca taagttttaa attcattttt gttaatatta ttaaggacaa
     2881 atctatcggc ttctacttca ttgtcatgaa ttatctttct ttttacaata tgtactaatg
     2941 gattgtaact cataataata gagaagatat taaagataat taaatgtaaa gtatctctat
     3001 ttttagcatg agcatattca tgcagtatta tatattttag tcttttatca attacacttt
     3061 taaaatatga actaggaatc aaaataatat atttcccata ccaaaaagtt attggagatt
     3121 gaatagcctc tgcttttcga atcacaatat tttttttata ttgatggttg aaaagtatcg
     3181 tatctatttt atttttttca ttttcgttta gataaagtga ctgtttcttt aaatatttaa
     3241 gatataataa ggctttcaaa aatttaaaac ttaaaataat aactaaaact atccaaataa
     3301 ctgtgcagat attatcaatt gaatcccaat taaacttatg gatatctgtt gtgaactctt
     3361 gaataggttt ggtggtattt atgttatggt tcaaatcgtg tgacttactt tcaactgtgg
     3421 gcgcttgatt attcacatta ttaaatttta taaaagagaa tttaattggg ataaaaggaa
     3481 ttaatccagc aagcatagtt agataccaaa ctttataatt taaactataa ttaaaatagc
     3541 gttttaatat gtacctaaaa aatactaaca atagaaatat aaaacaaaag cttactacgc
     3601 tcattattag taatttagcc attttgacac cttctttcaa atatttataa taaacaattg
     3661 acatcaatat tacaattgta atattattga tttataaaaa ttacaactgt aatatcggag
     3721 ggtttatttt gaaaaagtta atatttttaa ttgtaattgc tttagtttta agtgcatgta
     3781 attcaaacag ttcacatgcc aaagagttaa atgatttaga aaaaaaatat aatgctcata
     3841 ttggtgttta tgctttaaat actaaaagtg gtaaggaagt aaaatttaat tcagataaga
     3901 gatttgccta tgcttcgact tcaaaagcga taaatagtgc tattttgtta gaacaagtac
     3961 cttataataa gttaaataaa aaagtacata ttaacaaaga tgatatagtt gcttattctc
     4021 ctattttaga aaaatatgta ggaaaagata tcactttaaa agaacttatt gaggcttcaa
     4081 tgacatatag tgataataca gcaaacaata aaattataaa agaaatcggt ggaatcaaaa
     4141 aagttaaaca acgtctaaaa gaactaggag ataaagtaac aaatccagtt agatatgaga
     4201 tagaattaaa ttactattca ccaaagagca aaaaagatac ttcaacgcct gctgctttcg
     4261 gcaagacttt aaataaactt atcgcaaatg gaaaattaag caaaaaaaat aaaaatttct
     4321 tacttgattt aatgtttaat aataaaaacg gagacacttt aattaaagat ggtgttccaa
     4381 aagactataa ggttgctgat aaaagtggtc aagcaataac atatgcttct agaaatgatg
     4441 ttgcttttgt ttatcctaag ggccaatctg aacctattgt tttagtcatt tttacgaata
     4501 aagacaataa aagtgataag ccaaatgata agttgataag tgaaaccgcc aagagtgtaa
     4561 tgaaggaatt ttaatattct aaatgcataa taaatactga taacatctta tagtttgtat
     4621 tatattttgt attatcgttg acatgtataa ttttgatatc aaaaactgat tttcccttta
     4681 ttattttcga gatttatttt cttaattctc tttaacaaac tagaaatatt gtatatacaa
     4741 aaaatcataa ataatagatg aatagtttaa ttataggtgt tcatcaatcg aaaaagcaac
     4801 gtatcttatt taaagtgcgt tgcttttttc tcatttataa ggttaaataa ttctcatata
     4861 tcaagcaaag tgacaatagc attttacaag tattttttat tgttcgatta gggtaaacct
     4921 aaataataca taaattatat tacaatatgt ttattataga ggctaaagta tcttagaaat
     4981 ttggatagta ggtttttcgg gaataaaagt atagttggct ttaatatata attaagctaa
     5041 gctatcattt atatttactc gattataatt cgcacttcaa cttttttata acgtaaaaat
     5101 taaaacgtag ttttatttat ctttagtaat atctttttaa acactacagt ttcttcatta
     5161 gttaaattag cgaagtaatg attattaagt tgataggctt tcttttctaa tgttggccat
     5221 aagtcttttg ctttatttgt actgtttatt agcacagttc ttctattttc attatcagtt
     5281 tgacgtgtta ttagatcctt tttggacatc ttatctaata ttccagatat tgttgaaggt
     5341 tctaaattta aagtattacc aatcgcttta ggtgtattca aattactatt aatcaagcaa
     5401 ttcaaaacac catattctgc tggagtaata ttatagggac ttaataaact tttaaaatgt
     5461 ttaaaaactc tattctgaga agtactcaac aaataattta tacaattatc taattccaca
     5521 actaaaacct ctcttaaatc attagtattg actcgcttat catatcatga taagttttgt
     5581 aggaaaatag ttttgcaaca aaaacatact tctacaaaac aattgtgaaa gaaggctaat
     5641 aaatgttcaa tgcggtagta gtgagaaaaa gtgcatcaaa tgttagttat aaagtagaaa
     5701 aagttgatga atctcattta tcgaagggtg atgtattagt aaaagtggta tactcttcaa
     5761 taaattataa ggatatgcta gctttacaat ataaaggcgg tgtaattaga gattatccta
     5821 tgattcctgg tatagatttt gcaggtattg ttgaatcaag ttcgaatgat aagtttaaag
     5881 aaggcgataa tgtattagtt actggttatg atgttggcgt gacacataca ggtggatttt
     5941 cagaatatgc acaagtgcct ggagagtgga ttgttcctat tccccaagaa atgtctatta
     6001 aagagtctat ggtatatggc acagccggtt ttacggcagc attatcaatt tttgaactag
     6061 aaaaatatgg aatgaatatc aaatcacaac ctgaaatttt agtgtctggg gctacaggtg
     6121 gtgtgggcag tgttgctata caaattttac ataaaattgg atatgaaaat atcactgcta
     6181 tcgtaagaaa agaaaagcaa atagaaatcg caaaaaaatt aggtgcaaaa caagtaataa
     6241 taattgaaga tgataataaa ccgcttaaaa aaggtatttt tgattatttc ttagatacag
     6301 tggggagtaa tgtaacatta tacggtttaa aacgtctaaa ttatcaaggt gttgctactg
     6361 tatgtggaaa tgtagcagga aattcactta acgctaatat attaccgttt attcttagag
     6421 gtattaaatt aattggcatt gattctgtat atgttgatca taatatccgt gaagatatat
     6481 ggagtaagtt agcaaatgaa tggaatatag gaaattcact tctatacaat gaaattggat
     6541 ttgaagaatt ctataaaacg atagatcagc ttttaaaagg acaacattta ggaagaacta
     6601 ttttaaaagt ataagtgtga gtattcatta atacatttac tattaattca atcattttgc
     6661 atttctctac taaccagtgt tataatttat ataacgaaaa tgattaacaa ttaatgcacc
     6721 aatccccaca ctattgcagt agtgtgggga ttttttcgat gctggcttat aaagtcgcct
     6781 attaattatt tgttgcgttt actaagccaa taagtataat acgcaacagc acagccactg
     6841 atgactggtg ctatgatgtg aacgaaaa
//
LOCUS       DAMQYT010000480         6633 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.guided.139, whole genome shotgun sequence.
ACCESSION   DAMQYT010000480 DAMQYT010000000
VERSION     DAMQYT010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.guided.139"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            458..1924
                     /gene="msr(A)"
                     /locus_tag="PGA33_002954"
     CDS             458..1924
                     /gene="msr(A)"
                     /locus_tag="PGA33_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HDG6474669.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
     gene            2023..2922
                     /locus_tag="PGA33_002955"
     CDS             2023..2922
                     /locus_tag="PGA33_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HDG6474670.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            3408..4016
                     /locus_tag="PGA33_002956"
     CDS             3408..4016
                     /locus_tag="PGA33_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDG6474671.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            4262..4600
                     /locus_tag="PGA33_002957"
     CDS             4262..4600
                     /locus_tag="PGA33_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474672.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            4605..4790
                     /locus_tag="PGA33_002958"
     CDS             4605..4790
                     /locus_tag="PGA33_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDG6474673.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(5017..5760)
                     /locus_tag="PGA33_002959"
     CDS             complement(5017..5760)
                     /locus_tag="PGA33_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDG6474674.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            6186..>6633
                     /locus_tag="PGA33_002960"
     CDS             6186..>6633
                     /locus_tag="PGA33_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDG6474675.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENR"
BASE COUNT         2523 a          946 c         1023 g         2141 t
ORIGIN      
        1 tgttaaaatt atattataaa ttcaactttg caacagaacc gtattttatg agtacactct
       61 aaattaagga gatgtataat caatctaaaa ttgtattttt atacttttgt tatataatct
      121 ttctacttag agatatattg taaaaaaacc aacaaaatgt tatattaaca ttactatttt
      181 atcgatgaag gaggattaaa atgacagctt ctatgagact caaataaacc tttttgaata
      241 caaaactaaa ttaattcaaa aaggcttgtc cgcaatacac actcatagga tgacttatgt
      301 cattttttat ccttcgatag gaataataaa cgctttcaaa tactaaaaca tcataataaa
      361 tttttaagtg tttgaaagtg taatgtgaat gagacgggag tagcctgtct catttttttg
      421 cgtacacgtc ctttaaatat acaaggagtg tataaatatg gaacaatata caattaaatt
      481 taaccaaatc aatcataaat tgacagattt acgatcactt aacatcggtc atctttacgc
      541 ttaccaattt gaaaaaatag cacttattgg gggtaatggt actggcaaaa ccacattact
      601 aaatatgatt gctcaaaaaa caaaaccaga atctggaaca gttgaaacgg ttggcgaaat
      661 tcaatatttt gaacagctta acatggatgt ggaaaatgat tttaacacgt tagacggtag
      721 tttaatgagt gaactccata tacctatgca tacaaccgac agtatgagtg gtggtgaaaa
      781 agcaaaatat aaattagcta atgtcatatc aaattatagt ccgatattac ttttagatga
      841 acctacaaat cacttggata aaattggtaa agattatctg aaaaatattt taaaatatta
      901 ctatggtact ttaattatag taagtcatga tagagcactt atagaccaaa ttgctgacac
      961 aatttgggat atacaagaag atggcacaat aagagtgttt aaaggtaatt acacacagta
     1021 tcaaaatcaa tatgaacaag aacagttaga acaacaacgt caatatgaac agtatataag
     1081 tgaaaaacaa agattgtccc aagccagtaa agctaaacga aatcaagcgc aacaaatggc
     1141 acaagcatca tcaaaacaaa aaaacaaaag tatagcacca gatcgtttaa gtgcatcaaa
     1201 acaaaaaggc acggttgaga aggctgctca aaaacaagct aagcatattg aaaaaagaat
     1261 ggaacatttg gaagaagttg aaaaaccaca aagttatcat gaattcaatt tcccacaaaa
     1321 taaaatttat gatatccata ataattatcc aatcattgca caaaatctaa cattggttaa
     1381 aggaagtcaa aaactgctaa cacaagtacg attccaaata ccatatggca aaaatatagc
     1441 gctcgtaggt gcaaatggtg taggtaagac aactttactt gaagctattt accaccaaat
     1501 agagggaatt gattgttctc ctaaagtgca aatggcatac tatcgtcaac ttgcttatga
     1561 agacatgcgt gacgtttcat tattgcaata tttaatggat gaaacggatt catcagaatc
     1621 attcagtaga gctattttaa ataacttggg tttaaatgaa gcacttgatc gttcttgtaa
     1681 tgttttgagt ggtggggaaa gaacgaaatt atcgttagca gtattatttt caacgaaagc
     1741 gaatatgtta attttggatg aaccaactaa ttttttagat attaaaacat tagaagcatt
     1801 agaaatgttt atgaataaat atcctggaat cattttgttt acatcacatg atacaaggtt
     1861 tgttaaacat gtatcagata aaaaatggga attaacagga caatctcttc atgatataac
     1921 ttaaaaacta tacactaatt tatattgaat attaggttca tttatatcaa tataaaaaat
     1981 acgttcacaa gaaaaatata atagaaataa ggatccaata aaatgactcg acataatgaa
     2041 attattaaat gtgcagaaaa atatcaatta cacatccaac ctcaaacaat ctcattgaat
     2101 gaatcgggac ttgattttca agttgcattt ggaaaagata aacatggagt agaatgggtt
     2161 ttgagactac caagaagacc tgacgtttat aaacgaacaa aacccgaaaa acaaacggta
     2221 gacttcttac agaagaatgt ttcatttgaa ataccgaagt ggaaagtaca tgcaaaagac
     2281 cttattgctt acccaaaact tacaggtaaa cccgcagcca caatagatcc agaaatacaa
     2341 aattatgtat gggaaattga acacaaacca ttaccagaaa actttattaa cacattagct
     2401 gaaacactcg tagatttaca caacatacca gaagaaaaca ttaacgttca gcatataaat
     2461 atcaaaacca tacaagaaat aaaaaatgac tttcaaagaa gaatgaataa agttaaagaa
     2521 acttatggcg tatcagatga attatggaac agatggaaac aatggttaga aaacgacgaa
     2581 ctatggcctc gacatgcgac catgatacat ggggacttac atccaggaca tataatggta
     2641 gataaccaag caaacgtcac aggtctcata gactggactg aagcaaccca ctccgaccca
     2701 tcaatggact ttataggaca ccatcgtgta ttcgacgacg aaggattaga gcaacttata
     2761 acagcatacg gtaaagctgg aggtgaaata tggccacgaa tgaaagagca tataatagaa
     2821 ctcaatgcag tattcccaat gtttatcgct gagtttgcta tggaatcagg agaatcggcg
     2881 tatgaaacga tggcattgaa agagttaggt atgaaagagt agaacttata ttaaaagagg
     2941 cccattttaa agacgaaggc aatcctgatg aaattttaaa actgcgttta attagtgatg
     3001 gtctttggtt ttctataatg tattaaatat ctggacatgg aacaaatgca acaaatggaa
     3061 agaatcgttt tcgaaatttc taactcctta aatcaggagg ataaataaac ggtcattttc
     3121 attgaaagca cttatataaa tttcatcaat tttaatacct accgtaaata catttcataa
     3181 gtattaaaca gatgattaaa atttttagtt aatttaaata tttatgatag agatgacctt
     3241 caaatgaacg gttctgttac aaagaaaaaa tctatattta tttttagtaa gttttaatta
     3301 tagatatgta cactttgagt acaccctaac catacaaaaa gaattgttga aattataaca
     3361 attaaaacgt atgattaggg tgtatattta ttatggagtg aagatgaatg attataggtt
     3421 atgcgagagt atcatcgata gatcaaaatt tagaaagaca attggataat ttaaagacgt
     3481 ttggtgtgga gaaaatcttt acagagaaac agtcgggaaa gtcagtggaa aatagacctg
     3541 tatttcaaga agcactcaat tttgtcagaa tgggagatcg attcgttgtg gaatctattg
     3601 atcgtttagg acgtaactat gatgaaatta ttgaaactgt gaattattta aaagaaaaag
     3661 acgttcagtt aatgattacc agcttaccta tgatgaatga agtaattggc aatccattac
     3721 tggataagtt tatgaaagat ttaatcattc aaatattagc aatggtttca gaacaagaga
     3781 gaaatgaaag taaacgtcga caagcgcaag ggattcaagt tgcgaaggaa aaaggcgtat
     3841 ataaagggcg ccctttactt tattcaccaa atgctaaaga cccacagaaa cgtgttattt
     3901 atcatcgcgt ggtggaaatg ttagaagaag gcaaagcgat tagtaagatt gcgaaagaag
     3961 ttaatatcac aagacaaaca gtttatagaa ttaaacatga taagggatta tcttgataaa
     4021 ttgattgtca atgcaaatga gcgtctctca cagtgtctca aattccataa catattattg
     4081 aattgagatt ttaataatta tacaagaaaa aagagtacga gttcgcagct ttctatatag
     4141 taattgtttt actaataagg tgttttaaaa tatattataa tgtaaaaagt atttgacatt
     4201 tgtatcaaaa atatttacac tcataaatgt caaatacttt ttacatttat gaggtgttat
     4261 aatgaattta attcctttta ttattatttt aattttttct tgtttatgga cggctataac
     4321 taacaaatat ttaccttcag aaacattaga taaaaaagga caacaagatc gatacgatga
     4381 aagacaacgt aaaatgttta ttgaaatact ggctaaaagt tttatttgga ttgtatattg
     4441 tatgttattt acactagtgc ttaaatttac aggtttgtca ccaagtattg aaggatcttg
     4501 gttttctcaa tatccagaaa ttttctttat aattgtagca ctatttctta tgctgtttaa
     4561 ttactatact acaaataaaa aatatacatc taaaggttaa attaatgaaa aatagaatta
     4621 gagaatatag aaaacaaaaa ggaatttctc aagaaacttt aagtaaagaa ttaaaagtgt
     4681 ctaggcaaac tattaatgca atagaaaaca ataaatatga tcctacattg acattagcat
     4741 ttaagttggc aagattgttt gatacaactg tggatgaatt attttcttaa ccattcattt
     4801 atcacaaaaa gagagtgcga attcgcactc tctttttgtg tagtagtact attaaatttt
     4861 gtctcaataa cgaacgttta taacgcaatc atgatgagag tattaataca gttaattatt
     4921 tgaaatactt tagtttacat aatcgatgat taccagaagt tctcactata taaaagacac
     4981 taaaatttta taacttttag tgcctaatca attattttag ctaccaaaca aacgactcca
     5041 aaaccccttt ttaggttccg tttcttcact cttttcttct atatcactat cattgtcatt
     5101 tagattagct ggaacgtctt ctttttttgc ttcttcacct tgtgtacttt cgtcgctttg
     5161 acttccagat atatccttat attgaatctc tgtttcttta gtttcttcct gatctctgtt
     5221 aatatttaca ggattagtag tatatgtttt ttcttgtgca tcaacgtttt gtctagtgtt
     5281 agtagatgta tcaaagggat aattaaggtg tttttctttt tctaattgat tttctaattt
     5341 ttgaatcttt ttattactct ctaatgccag aacttgctga ttttcaagca attttgatct
     5401 ttcttgcaac tcttttctgt aaaattcaat tgttgtttca tgacgacttt cttgtttttc
     5461 aatttgatta tttaaagtat caatctgatt tttaaggatt tctactattt gagcatcgct
     5521 tttcgattca aaattaatat tatccgaccc ttttttttgt ttgtcttcag tagtactttc
     5581 aaaaccatat ttctttttat tttcatttac tctttgaatg attttttcta tatcggtatc
     5641 ttctttaata aacgaagtgt tttcctgttt tattgtctct atattcaatc gcttaatatt
     5701 attaaaaatc gtttgcttac taacccctaa ttcttcactc aactttttca cacttttcat
     5761 atcatcaacc ctttataaaa tgtttataaa cccttgataa aatattataa gcttataatg
     5821 cttaataata aagtgattaa taaataaaaa taaacatcca tcaacctttt tatcaagggt
     5881 ttatgaaagg tttatgatat ttatcaaggg tttataaacc cttgataaag ctatattttc
     5941 ccttttataa gtttatttaa agtgctataa taaacttaat ataacaaaaa ccccaactac
     6001 tggtaatagt cggggtttgg taataaaacg tttagaaact gtttatttat ataattttcg
     6061 atacttgtat tatataatga ataatatctt agtgcaagca cctaaatcat taaataaatg
     6121 taaagctaaa taaaaacgtt ttattacctc ttaccaaact aaaatatgga aagaggtttt
     6181 ttgtcatgca aaatcaatat tttacagtgc aagaaaatta caaagaaaga ttttatcaaa
     6241 tccctaaagt attctttacc agtgaaaatt ataaaaatct aactaatgat atgaaaatag
     6301 cctatgctat cttacgtgat cgcttaaatt tatctattaa aaatagctgg gttgatgaag
     6361 atggtaacat ttactttgtt tattcaaatg aaaagctaat ggaaatctta aattgtaaaa
     6421 aagaaaagtt aacaaaaata aaaaaagggt tagaaaacga tggtctactc attcaaaaac
     6481 gacgtggttt aaataaacca aatattctat atttaatgaa acctattgtc acagaacgtg
     6541 atatttacaa aatagaaaaa gaagaaaacg atgttgaacc ttacggcgag aaggaagttc
     6601 gaaaatcgaa cgtccagaag ttcgaaaatc gaa
//
LOCUS       DAMQYT010000481         5357 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.guided.169, whole genome shotgun sequence.
ACCESSION   DAMQYT010000481 DAMQYT010000000
VERSION     DAMQYT010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.guided.169"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            <1..254
                     /locus_tag="PGA33_002961"
     CDS             <1..254
                     /locus_tag="PGA33_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HDG6474676.1"
                     /translation="VFGFMKAILGFTRMSVRGLNKVKRELGFVLMALNIRKVVAQRAE
                     NNQKIYKKDNFYIISIEIVFFHLSKNFMSRTHYDLFFYC"
     gene            complement(245..505)
                     /locus_tag="PGA33_002962"
     CDS             complement(245..505)
                     /locus_tag="PGA33_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HDG6474677.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(477..1463)
                     /locus_tag="PGA33_002963"
     CDS             complement(477..1463)
                     /locus_tag="PGA33_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HDG6474678.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            1560..3569
                     /gene="mecA"
                     /locus_tag="PGA33_002964"
     CDS             1560..3569
                     /gene="mecA"
                     /locus_tag="PGA33_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HDG6474679.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWNHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(3615..4043)
                     /locus_tag="PGA33_002965"
     CDS             complement(3615..4043)
                     /locus_tag="PGA33_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HDG6474680.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(4140..4883)
                     /locus_tag="PGA33_002966"
     CDS             complement(4140..4883)
                     /locus_tag="PGA33_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HDG6474681.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
BASE COUNT         1883 a          867 c          787 g         1820 t
ORIGIN      
        1 ctgtttttgg atttatgaag gctattttgg gtttcactcg gatgtctgtc cgaggactca
       61 ataaagtcaa aagagaactt ggttttgtat taatggcact taatataaga aaagtagtag
      121 ctcaacgagc tgaaaataat caaaaaattt ataaaaaaga caatttctat attatttcaa
      181 tagaaattgt cttttttcac ttatccaaga actttatgtc ccggactcat tatgatttat
      241 ttttttattg ctgacttgcc attaattctg cttgctctac aacttgttcc acagccattt
      301 tttgaagatc tggtggatag ccatatttct ttaataggcg tctaacttga actctcattt
      361 tagctttagc actgtctcgt ttagaccaat caacactcat gttatcctta actgttttag
      421 ttaactcatg tgcaatggct cttaactctt tatcgcccaa agcttcttta gctgtttcat
      481 gtgaagctcg atatactaca tacaatatac gcaacaatac gcttgtttcg attaggttta
      541 ggcattgaat cataaagtgc aatatactta acacgttctt taatatttga attaaaacct
      601 agtaaatatt gtgctgccac attatttatg tgctgagatt ttaaaataga gcattttaat
      661 atcgattcac cataacgtat atgttcatgg cgattcaaaa tttttaaaac gtttctatca
      721 catacttttt cacagtcatt gtccatcatt gttttactta tatatagtgc aggattaaac
      781 cagaatatca ttttaaaaac aacataaagc tggttgaata ttaagtcatg acttttcaca
      841 tgtgatagtt catgtagaat aatatattca atttctttgt cattcatggt ttcgactacg
      901 acagttggta gtacaatttg ggatttcact aaaccaaata ccatcggatt atcaatgttt
      961 gaactataac taattgttat atgctttttg tagaactgca tcttactttg acatacttta
     1021 agtcgttcat taagatatga cgattccaat gacgaacttt taataacatc aatttgtcgg
     1081 aatgccttaa tcatataaaa taagcacaac aaactaccaa atacccatat caaaagaatc
     1141 atatacgtta tatttgaggt ctcaaactga ttaacattaa ttgctaagtc tttcgtaaca
     1201 gatgattgtt gaccatctaa catatgacta accgaagaag tcgtgtcaga tacatttcga
     1261 ttcatcatat cttttgaaaa tgtaaaattc gatattttgt aaaatggtat taatggaatt
     1321 aacgtggaga cgagcactaa taaccaaatc ttatgtgaca taatattttg agtatatttt
     1381 atatagagca ttctcactaa aaaaattaca catatcgtga gcaatgaact gattatactt
     1441 aacattaaaa aagatgataa caccttctac acctccatat cacaaaaatt ataacattat
     1501 tttgacataa atactacatt tgtaatatac tacaaatgta gtcttatata aggagtatat
     1561 tgatgaaaaa gataaaaatt gttccactta ttttaatagt tgtagttgtc gggtttggta
     1621 tatattttta tgcttcaaaa gataaagaaa ttaataatac tattgatgca attgaagata
     1681 aaaatttcaa acaagtttat aaagatagca gttatatttc taaaagcgat aatggtgaag
     1741 tagaaatgac tgaacgtccg ataaaaatat ataatagttt aggcgttaaa gatataaaca
     1801 ttcaggatcg taaaataaaa aaagtatcta aaaataaaaa acgagtagat gctcaatata
     1861 aaattaaaac aaactacggt aacattgatc gcaacgttca atttaatttt gttaaagaag
     1921 atggtatgtg gaagttagat tggaatcata gcgtcattat tccaggaatg cagaaagacc
     1981 aaagcataca tattgaaaat ttaaaatcag aacgtggtaa aattttagac cgaaacaatg
     2041 tggaattggc caatacagga acagcatatg agataggcat cgttccaaag aatgtatcta
     2101 aaaaagatta taaagcaatc gctaaagaac taagtatttc tgaagactat atcaaacaac
     2161 aaatggatca aaattgggta caagatgata ccttcgttcc acttaaaacc gttaaaaaaa
     2221 tggatgaata tttaagtgat ttcgcaaaaa aatttcatct tacaactaat gaaacagaaa
     2281 gtcgtaacta tcctctagga aaagcgactt cacatctatt aggttatgtt ggtcccatta
     2341 actctgaaga attaaaacaa aaagaatata aaggctataa agatgatgca gttattggta
     2401 aaaagggact cgaaaaactt tacgataaaa agctccaaca tgaagatggc tatcgtgtca
     2461 caatcgttga cgataatagc aatacaatcg cacatacatt aatagagaaa aagaaaaaag
     2521 atggcaaaga tattcaacta actattgatg ctaaagttca aaagagtatt tataacaaca
     2581 tgaaaaatga ttatggctca ggtactgcta tccaccctca aacaggtgaa ttattagcac
     2641 ttgtaagcac accttcatat gacgtctatc catttatgta tggcatgagt aacgaagaat
     2701 ataataaatt aaccgaagat aaaaaagaac ctctgctcaa caagttccag attacaactt
     2761 caccaggttc aactcaaaaa atattaacag caatgattgg gttaaataac aaaacattag
     2821 acgataaaac aagttataaa atcgatggta aaggttggca aaaagataaa tcttggggtg
     2881 gttacaacgt tacaagatat gaagtggtaa atggtaatat cgacttaaaa caagcaatag
     2941 aatcatcaga taacattttc tttgctagag tagcactcga attaggcagt aagaaatttg
     3001 aaaaaggcat gaaaaaacta ggtgttggtg aagatatacc aagtgattat ccattttata
     3061 atgctcaaat ttcaaacaaa aatttagata atgaaatatt attagctgat tcaggttacg
     3121 gacaaggtga aatactgatt aacccagtac agatcctttc aatctatagc gcattagaaa
     3181 ataatggcaa tattaacgca cctcacttat taaaagacac gaaaaacaaa gtttggaaga
     3241 aaaatattat ttccaaagaa aatatcaatc tattaactga tggtatgcaa caagtcgtaa
     3301 ataaaacaca taaagaagat atttatagat cttatgcaaa cttaattggc aaatccggta
     3361 ctgcagaact caaaatgaaa caaggagaaa ctggcagaca aattgggtgg tttatatcat
     3421 atgataaaga taatccaaac atgatgatgg ctattaatgt taaagatgta caagataaag
     3481 gaatggctag ctacaatgcc aaaatctcag gtaaagtgta tgatgagcta tatgagaacg
     3541 gtaataaaaa atacgatata gatgaataac aaaacagtga agcaatccgt aacgatggtt
     3601 gcttcactgt tttattatga attattaata agtgctgtta cttctccctt aaatacaatt
     3661 tcttcatttt cattgtatgt tgaaagtgac actgtaacga gtccattttc tttttttatg
     3721 gatttcttat ttgtaatttc agcgataacg tacaatgtat tacctgggta tacaggttta
     3781 ataaatttaa cgttattcat ttgtgttcct gctacaactt cttctccgta tttaccttct
     3841 tctacccata atttaaatga tattgaaagt gtatgcatgc cagatgcaat gataccttta
     3901 aatctacttt gttctgcttt ttctttatct atatgcatat attgaggatc aaaagttgtt
     3961 gcaaattgga taatttcttc ttctgtaata tgaaggcttt ttgttttgaa tgtttctcct
     4021 actataaaat catcgtattt catatatgtc tctctttctt attcaaatta attttttagt
     4081 atgtaacatg ttaaaggtaa gtctaccgtc actgaaacgt aagactcacc tctaactttc
     4141 tattgagaca aatgcaccat tttatctgca ttgtctgtaa agataccatc aactccccaa
     4201 ttagcaagtt ggtttgcacg tgctggtttg tttacagtcc atacgttcaa ttcataaccc
     4261 gcttctttta ccatttttac ttttgcttta gtaagtttgg catcttcagt gtttactatt
     4321 ttagcattac agtaatctaa aagtgttctc cagtcttcac gaaacgaagt tgtatggaat
     4381 ataactgctc tgttatattg tggcatgatt tcttctgcaa gtttaacaag cacaacatta
     4441 aagcttgaaa tgagcacttc ttgattctga tttaagtttg ttaattgttc ttccacttgc
     4501 ttaaccatac ttttagaaag tgctagtcca ttcggtccag taataccttt taattctaca
     4561 tttaaattca tattatattc atttgctatt tttactacat catcgaaagt tggcaaatgt
     4621 tcatctttga atttttcacc aaaccaagat cctgcagaag catctttaat ttcatcataa
     4681 ttcaattcag ttatttcccc ggacatattt gtagtccgtt ctaaataatc atcatgaatg
     4741 ataatcagtt gttcatcttt tgtaattgca acatctaact ccaaccagtt tataccttct
     4801 acttctgaag cagctttaaa tgatgcaatt gtattttccg gagctttact aggtaatcct
     4861 ctatgtccat atacagttag catattacct ctccttgcat ttttattttt ttaattaacg
     4921 taactgtatt atcacattaa tcgcactttt atttccatta aaaagagatg aatatcataa
     4981 ataaagaagt cgatagattc gtattgatta tggagttaat ctacgtctca tctcattttt
     5041 aaaaaatcat ttatgtccca agctccattt tgtaatcaag tctagttttt ctgtacccct
     5101 tatctgcaat tttacttagg attgctttta acttacccct tatcagcaat tttactgaga
     5161 actgctttta acgcacctct tatctgcaat tttgcctaga actgctttta acgtacctct
     5221 tatctgcaat tttactgaga actgctttta acttacccct tatcagcaat tttgcatgga
     5281 attgctttta acgtacctct tatctgcaat tttacttaga actgctttta acaaacctct
     5341 tatctgcaat tttactt
//
LOCUS       DAMQYT010000482         4774 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.guided.188, whole genome shotgun sequence.
ACCESSION   DAMQYT010000482 DAMQYT010000000
VERSION     DAMQYT010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.guided.188"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            51..935
                     /locus_tag="PGA33_002967"
     CDS             51..935
                     /locus_tag="PGA33_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDG6474682.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            1057..1476
                     /locus_tag="PGA33_002968"
     CDS             1057..1476
                     /locus_tag="PGA33_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HDG6474683.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(1732..2667)
                     /locus_tag="PGA33_002969"
     CDS             complement(1732..2667)
                     /locus_tag="PGA33_002969"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HDG6474684.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            2957..4216
                     /gene="sdpC"
                     /locus_tag="PGA33_002970"
     CDS             2957..4216
                     /gene="sdpC"
                     /locus_tag="PGA33_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HDG6474685.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            complement(4282..>4774)
                     /gene="rpiA"
                     /locus_tag="PGA33_002971"
     CDS             complement(4282..>4774)
                     /gene="rpiA"
                     /locus_tag="PGA33_002971"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HDG6474686.1"
                     /translation="ELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGGALFREKV
                     IDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKVERRVNED
                     VAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVGTQEGVKI
                     LEK"
BASE COUNT         1687 a          858 c          677 g         1552 t
ORIGIN      
        1 ataaactaat tatccatagc ttgaatctat aaataaaagg agcaaaacac atgaaaatta
       61 ttcagttaga atacttcttg gctatcgtga aatataatag ttttactaaa gctgcacaat
      121 ttttacatat tagccagcca tctttaactg ctacgattaa aaaaatggaa gcagatttag
      181 gttatgactt atttacacgt tcaacaaaag acatcaagat taccgaaaaa ggaatacagt
      241 tttatcgtta tgcgagcgaa ttagttcaac aatatcgatc cacgatggaa aaaatgtatg
      301 atttaagcgt tacatcagaa ccaaggataa aaattgggac tcttgaatct acgaatcaat
      361 ggattgcgaa tttaattcga aagcaccatt ccgactaccc tgaacagcaa tatcgtttat
      421 atgaaataca tgataaacat caatctatag agcaattact gaattttaat attcatttag
      481 ctataacaaa tgaaaaaata acccacgaag atataagatc cattccttta tatgaggaat
      541 cttacatttt attagcaccc aaggaaacat ttaaaaatca aaattgggta gatgttgaaa
      601 atttgccact catattacca aacaaaaatt ctcaagtgcg caaacactta gatgactatt
      661 ttaatagaag aaatattcgt ccaaatgtcg ttgtagaaac agatcgattc gaatcagcag
      721 ttggatttgt tcatctcggc ttaggttacg ctatcattcc gagattttat taccaatcat
      781 ttcacacgtc taatttagaa tataaaaaaa ttcgtccaaa cttaggccga aaaatttata
      841 tcaattacca taaaaaacgc aaacactccg aacaagtaca tacattcgta caacaatgcc
      901 aagattattt atatggactt ttagaggctc tttaacttaa gttattagag cctcttatgc
      961 agttgcgcag atcatcgtat aaaaattaat gacgtcattt caaaaatcga tacaaaaata
     1021 atttattata aaaattctaa gaaagaagtg aagcagatgt taaaatctat taatcatata
     1081 tgcttttcag tcagaaattt aaacgattca atacattttt atagagatat tttacttggg
     1141 aaattgctat tgactggtaa aaaaactgct tattttgagc ttgcaggcct atggattgct
     1201 ttaaatgaag aaaaagatat accacgtaat gaaattcact tttcatatac acatatagct
     1261 ttcactatag atgacagcga atttaaatat tggcatcaga ggttaaaaga taataacgtg
     1321 aatattttag aaggaagagt tagagatatt agagatagac aatcaattta ctttaccgac
     1381 cctgatggtc ataagctaga attacatact ggcacacttg agaacagatt aaattattat
     1441 aaagaggcta aaccacatat gacattttac aaataaggtg tcattataaa aaggcctctt
     1501 gaactccgtt aaaattttaa ttaattatta tataataaga gaacttttca aacaatacag
     1561 ttgttatttt tgctatttca acaaacataa ataagcagta agatgactac aacttaagag
     1621 tcttcttact gcaattattt ttcaaatata tcaacgttaa tataacttct attaagaaat
     1681 actcacattc tgccctgcaa tgcaaatctc gtcacatata aatattttta attattttaa
     1741 aaaatgatgc actaaattag caacgagctt agcagttcta ttgtcagcgt catatgttgg
     1801 attcatctca gcaatactaa ctgaagacac cttatcactt ggaataatac gttttgctaa
     1861 ttcaagaaca gtatgtggat acaaacctaa cactgccggc gcacttaccc caggcgcaaa
     1921 cgcactatca atgacatcca tacaaatcgt aaacataatg acatcatgtt catgtacaaa
     1981 acgttcaatc atatctttaa ttgttggtga tacgtgactc aataattcat ctgcaaagac
     2041 ataatcaatc tttttctctt tagcataatc aaataaactt tgcgtattac caccttgagc
     2101 aataccaagc actaaataat ctgtgttttc atcttcttct aaaatttgtc taaagctcgt
     2161 tccagatgta gattgttgtt cagcacgtgt atcaaaatgc gcatcaatat ttatcacacc
     2221 aatagattgt gttggataga ctttacgtgt tgctaaatat tgagcatacg caatatcatg
     2281 tccaccacct aataaaaatg tttgtctatg attagcaatt gacttcgctg caagcatagc
     2341 aaattctttt tgagtatcaa ttaattcctc atgatcatga taaacatttc cgtaatcgac
     2401 taaagtttca cattgattca aatccggcaa acctgcaaat gcttgtttaa tcgcatctgg
     2461 tccttctttt gcaccaatgc gccccttgtt taaagcaaca cctttgtcaa cagcatagcc
     2521 taatataccg acccctgatg gcatactact cttttccagc ttagacaaat cttcaaatgt
     2581 tactgtttga aaatgtctaa attttttcgg gtctgtttca ctatctaacc ttccagtcca
     2641 taaatttggt tcaccttgct tgtacacagc atttccccct cttatttatg tggcttatta
     2701 acaattaaag tataacgtat aggaaatttt gaattcaatt catagttaaa tccgtatctt
     2761 aaaaatactt atctacatta cttttacccc tattttctat gtaataacga atacttagct
     2821 gatttatgtt aataaaatac gtcaagacta ttacattttc attaatattg acatagacaa
     2881 tttatctctc ggcttgtaat atgtataatt gttactaaaa gatattttgc ttgttaccta
     2941 atggaggtta catataatga agaacaataa aatttctggt tttcaatggg caatgacgat
     3001 tttcgtcttc tttgtcatta caatggcgtt atccattatg ctcagagatt tccagtctat
     3061 aattggtgtc aaacacttta tatttgaagt tacagatcta gcaccattaa ttgctgcaat
     3121 catttgtata ctcgttttca aatataaaaa ggtccaactt gcaggtttaa aattctcaat
     3181 cagcctgaaa gtaattgaac gtctattgct agctttaatt ttacctttaa ttattctaat
     3241 tattggtatg tacagcttta atacatttgc agatagcttt attttattac aatcaacagg
     3301 cttatcagta cctattacac acattctgat tggacatatt ctgatggcgt tcgtagtaga
     3361 attcggattc cgttcatact tacaaaatat tgttgaaacg aaaatgaaca cgttctttgc
     3421 aagtattgtt gttggtttga tgtattcagt tttctcagca aatacaactt atggtacaga
     3481 atttgctgct tataacttcc tttatacatt ctcattctct atgattcttg gtgaattaat
     3541 tagagcgact aaaggacgta caatttatat tgcaacgaca ttccatgctt caatgacatt
     3601 cggacttatt ttcttgttta gcgaagaaat cggcgatcta ttttcaatca aagtcatcgc
     3661 catttcaaca gcaatcgttg cagtaggata cattggttta agcttaatta tccgaggtat
     3721 tgcatattta acaacaagac gaaaccttga agaacttgag cctaataatt atttagacca
     3781 tgtcaatgac gatgaagaaa ctaatcatac tgaggctgaa aaatcttctt caaatattaa
     3841 agatgctgaa aaaacaggtg tagctactgc atcaacggtt ggtgttgcta aaaatgatac
     3901 tgaaaataca gtggctgacg aaccaagcat tcatgaaggt actgaaaaaa cagaacctca
     3961 acatcacata ggtaatcaaa ctgaatctaa tcatgatgaa gatcatgaca tcacttcgga
     4021 gtcagtagaa tcagcagaat cagttaaaca agcaccacaa agtgacgatt taacaaacga
     4081 ttcaaatgaa gatgaaatag agcaatcatt aaaagaacct gcgacttata aagaagacag
     4141 acgttcatca gttgtaattg atgcagaaaa acatatcgaa aaaactgaag aacaatcttc
     4201 agataaaaac aaataataac aaagcgcttg ctagtacctc ttaaaaagat gatgctagca
     4261 agcgcttttc tatactatat attatttttc taaaatttta acaccctctt gagtgcctac
     4321 aataacttga tctgccatat ctaagaagta tcctgtctca aacacacctg tcagatgaat
     4381 taaatactca tggaacttat atgggtcaat tccttttggt aacttacaat ctaaaatata
     4441 attgccatta tcagttataa atgcgacatc ttcgtttaca cgacgttcta cttttatatc
     4501 agcgtatgat tcaattttac gtaatatgtg ataccagtta aatttatcca cctctactgg
     4561 taacttaaac gtctcaccta agtattgaac tattttcgtt tcatcgacaa ccacaacaaa
     4621 acgcgatgcc atttcatcta taactttctc tctgaacagc gcaccaccgc cacctttaat
     4681 tatatttaaa gatggatcta cttcatcagc accatcaatt gctaagtcga tatgatcaac
     4741 atcattgatt tcacatattt taatacctaa ttct
//
LOCUS       DAMQYT010000483         3299 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.guided.227, whole genome shotgun sequence.
ACCESSION   DAMQYT010000483 DAMQYT010000000
VERSION     DAMQYT010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.guided.227"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            39..416
                     /locus_tag="PGA33_002972"
                     /pseudo
     CDS             39..416
                     /locus_tag="PGA33_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            413..1294
                     /locus_tag="PGA33_002973"
                     /pseudo
     CDS             413..1294
                     /locus_tag="PGA33_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1410..2339
                     /locus_tag="PGA33_002974"
     CDS             1410..2339
                     /locus_tag="PGA33_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDG6474687.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFEDLVGGGTIG"
     gene            2332..3069
                     /locus_tag="PGA33_002975"
     CDS             2332..3069
                     /locus_tag="PGA33_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDG6474688.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKV
                     PTPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLKNLLIIPISRESFL
                     VSKIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSP
                     PIIILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFL
                     LSLIIIIVTSIFSVIILFSYFKKTDIS"
     gene            3083..3286
                     /locus_tag="PGA33_002976"
     CDS             3083..3286
                     /locus_tag="PGA33_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDG6474689.1"
                     /translation="MGFIEQIKAFILGVVEGLTEFAPVSSTGHMIIVDDMWLNTADDL
                     GSASATTFKVVIQLGGSVAKLNL"
BASE COUNT         1201 a          433 c          587 g         1078 t
ORIGIN      
        1 attagtgatt agctatattt tttactttgc aacagaacca gccatttaat atgactgtta
       61 caattactaa gattcaagca ctattacgcc gcacgtatga tttcaccact caagttaata
      121 atttaacagt agatgattgc gagttactac ttgatgcagc aaagcttaaa tttaatgaag
      181 aaaccataga cctgacacac actgaattgc aaatcatgca tagcttattc cagaacaagg
      241 gcaaattcgt cagtcgtaat gcgttaatcg aaaaatgctg ggaatcagaa cacttcattg
      301 atgacaatac gcttgctgtc aacattacac gtttacgcaa aaagctacaa aaactaggac
      361 ttacagattt tatcgaaacg aagaaaaatg tcggatatcg tgtaggtgac gtatgaattt
      421 cttgaaatca atccaacgtg aaatttatat tgtcatcctt acttgtgctt tattctactt
      481 tatattattc atgtataatg tgccgatgca agcatatatg attggtctag gtattgtatt
      541 gtttgtttca atgatttact ggggtatcca ttacatgaac tttaaaaaag aataaacact
      601 acaggaacag aacgaagatt taagaatcga aaaccataaa cttcgtaatg actttattaa
      661 ctaccaaagt gagttagagt catatttttt aatgtgggtg catcaaatca agacaccgat
      721 tacagcatca aagttactgc ttgaagatcc tgatgaacac gtgatttcta acgtgcgtaa
      781 tgaagtctta caaattgata attacacgaa tcttgcactg aactatttga aactcatgaa
      841 ccaaaagaca tacatggtct ttatggaagt gagtgtagat gaattaattc gaccactgat
      901 taaacgttat gcgatacagt ttattcatca tcaaacaaag ttgcattatg agaaaagtga
      961 agcggttata ttaacagatg ccaaatgggc atcaattatg attgagcaaa tattaaacaa
     1021 tgcattaaaa tatgcacgtg gcaaagatgt gtggattacg tttgacgaaa atacaaagaa
     1081 attatcaatc cgagataacg gtatcggcat taatgcgagt gatttaccga aaatctttga
     1141 taaaggtttc tcgggctata acggtagaat aagtgagaaa tcaagcggta ttggtttata
     1201 tattgtcagc acaatctcaa agcgcttagg ccataaagta agtgctacat ctacattaaa
     1261 cgatggttca atattcacca ttcaatttaa ataactccaa tctttcattt ttgtaagatt
     1321 agcggctgtt tttgtaagaa tagataaagg cagccttttc taatcgcgcg taagataaag
     1381 gtaacaacaa actaagaaag gtgccagagg tgaaaaattt gaataatata attgaaataa
     1441 ataacttaac aaagcaatat ggaagtcaga tagcagttaa taatttgagt ataactgttg
     1501 aaagtggaca aatttatggt tttttaggaa gaaatggtgc aggcaaaaca actactataa
     1561 gaatgctttt aggcttaatt aaacctacga aaggtaatat taaaatattt gaaaaagagt
     1621 tcgaaagtaa taaaactgaa attttaaaaa atattggtgc tatggttgaa acgccaggtt
     1681 tttatagtaa tttgacagct aaagagaact taactataaa tgctaaaata ttaggtatac
     1741 aaaaagaaaa tgcaattgaa gaagtgttag aaatagtagg actagcttct gaaaaagtaa
     1801 aattgtttaa aaattattct ttaggtatga agcaacgttt agggattgcg agagctttat
     1861 tgcacaatcc cgaattattg atacttgatg agccgacgaa tggattagat cctattggca
     1921 taaaagaaat tagaaagttg attaaggatc tggctcaatc tagaaatata accattttaa
     1981 tttcaagtca tatattgtca gaaatagagc agttagtaga caaggtgggt attattcata
     2041 acggagtttt gttagaagaa attatgtatg aagatttaaa aaaaaagaac agacaattta
     2101 taagtatatc agtatcagat gaagaaaaag ttgcatgttt gttggaaact aacttcggaa
     2161 tttatgacta taaaattaaa gaaaatggcg aatttaaaat ttattcacat attgatagac
     2221 aacaagaaat taatagatta ttagtattaa acaatattga tgttttccag atgagagtga
     2281 tagaagataa tctagaaact tatttcgaag acttagtagg aggtggcaca attggataat
     2341 ctactccgta cagaatttat taagttaaaa agatcatata tgtttctaat aagtatttta
     2401 ggtacatgcg tagcaccttt tatggtggtt gtagcttctt ttattagtat gcggacgaaa
     2461 gtaccgactc ctaatattgt atttaaacag ttatttgaag aagtcagttt atatcatatg
     2521 ctaattattg gacctcctct atatggggtt ataatagcct atttgtttgg aagagaatat
     2581 atagaagata ctttaaaaaa cttattaatt atacctatat ctagagaaag ttttttagta
     2641 agtaaaataa tattattatt cttgtggatt caaacactat ctgttattgc atggttgcta
     2701 actttaatgc taggtatgct attgcatttt caagggttaa gccagagttt aataattgat
     2761 tcattcttta catttgttat tggcggagtg ttattatgga tattatctcc accaataatt
     2821 attttgtcga tatattttaa aaattatgtt cccacaattg ttttaaccat aataattact
     2881 ttgataaatt taatgactgc taattctgaa catagaggat tatttccttg ggctgcagtt
     2941 ggagacatat ctagaggaac attacctaat acttatccat ttctattatc tttaattatc
     3001 ataattgtta catctatatt cagtgtaatt atattattct catactttaa aaaaactgat
     3061 ataagttaaa aggaggagaa aaatgggttt tatagagcag attaaagcat ttattttagg
     3121 tgtagttgaa gggttgacag aatttgctcc agtatcatca acaggtcata tgattattgt
     3181 tgatgatatg tggttaaaca ctgctgacga tttaggaagt gcgtctgcaa ctacttttaa
     3241 ggtggtaata cagttaggag gttctgttgc aaagttgaat ttataatata attttaaca
//
LOCUS       DAMQYT010000484         3215 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.guided.233, whole genome shotgun sequence.
ACCESSION   DAMQYT010000484 DAMQYT010000000
VERSION     DAMQYT010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.guided.233"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            complement(17..772)
                     /locus_tag="PGA33_002977"
     CDS             complement(17..772)
                     /locus_tag="PGA33_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDG6474690.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            1008..1715
                     /gene="deoD"
                     /locus_tag="PGA33_002978"
     CDS             1008..1715
                     /gene="deoD"
                     /locus_tag="PGA33_002978"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDG6474691.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            1722..3074
                     /gene="tet(38)"
                     /locus_tag="PGA33_002979"
     CDS             1722..3074
                     /gene="tet(38)"
                     /locus_tag="PGA33_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDG6474692.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
BASE COUNT         1017 a          486 c          577 g         1135 t
ORIGIN      
        1 tatattttaa taatttttaa taatccgatt gtcttatacg tgtcagtgtt aattcagata
       61 tttcctgtgg aatataccac ttattaatca taattggata aggtgtttgt gcgtacagtg
      121 tttcaataat cagccaacaa tgtgtatcac catcaaacac gtgactatga tttttgaagt
      181 ggggcgcttt ggtaatagac atttttaaat ctgattgata tgcattgcta taaatcgttt
      241 gctcaacgaa tgtcttcatg tcgtcttcgt tttgtgtatt cactttaaat gtgtcaatga
      301 catttaacgg tataaaggta aagcaaaatg catcagcttg cttagaatga ttgtcctttt
      361 tttgataata gcgttccatt gcaatgacgg cagaaggatg gtttgcaaac aaatgatttg
      421 tatattcact ttctaaatca acacgataat taattgatga catagatacg cgagctagca
      481 atatttgatc aagtggatgc ttaaattgat ccatacttga agcgtgttgg gcatttgttt
      541 gtggaataac aaagtgtccc ttccctcttg tactctctac gatgccatct tcggctaaca
      601 attttatagc ttggcgcaaa gtcatacgac tgacatcaaa gcgcgcacaa agttcctttt
      661 cagtaggtaa tgcatggcca ctcggatatt ttcctatttg aatttcttta tataacgtat
      721 tataaatcgt taaaaatttt ggttgtgttt gcgtcacgta gacaacctcc ataaagttac
      781 ttaatcactc tcatcataca ataattttta ctcaaattgg aaaaattata aaaattaaat
      841 atagataggc tttgaaaatt agttttatac aaggttagta gctgtaactg taaaatgttc
      901 ttaatattgt caaaatgtaa tgcttgaaag cgcttttaaa aaatattatt atatacatgg
      961 ttagacaaat agacaaatca ctatacaaat attgggagga atattttatg aaatcaacac
     1021 cacacattaa accaatgaat gacgtcgaaa ttgcagaaac ggttctattg ccaggagatc
     1081 cgttaagagc taagttcatt gcagaaactt atttggatga tgtggaacag ttcaatacag
     1141 tgcgaaacat gtttggtttt accggaacat ataaaggtaa aaaagtttct gtcatgggtt
     1201 caggtatggg tatgccatct attggcattt actcttatga attaattcat acatttggtt
     1261 gtaaaaaatt aattcgcgtt ggctcttgtg gcgcgatgca agaaaacatt gatttatatg
     1321 atgtgattat tgcacaaggt gcctctactg attcaaatta cgttcaacaa tatcaattac
     1381 caggtcattt tgcgccaatt gcttcttatc aattattaga aaaagcagtt gaaacagcac
     1441 gtgacaaagg tgtacgtcat catgtaggta atgtgttatc aagtgatatt ttctataacg
     1501 cggatacaac agcgagtgaa cgttggatgc gtatgggtat tttaggtgta gaaatggaat
     1561 cagctgcatt atacatgaat gcaatttacg ctggtgtcga agcattaggt gtgttcacag
     1621 tgagcgatca tttaattcat gaaacgtcaa caacacctga ggaaagggaa cgtgcattta
     1681 cagatatgat tgaaattgca ctgtcattgg tgtagatgat tatgaatgtt gaatattcta
     1741 aaataaagaa agcagtacct attttattat tcttatttgt attcagtttg gttatagaca
     1801 actcatttaa attgatttct gtagccattg ctgatgactt aaacatatct gtaacgacag
     1861 taagttggca agcgacatta gccggtttag taattggtat tggcgctgta gtatacgctt
     1921 cattatctga tgccattagt atacgcacac tatttattta tggcgtgata ttaatcatta
     1981 tcggatcaat tattggttac attttccaac atcaattccc attactttta gttggacgta
     2041 ttattcaaac tgccggttta gctgctgcag agacattata tgtgatatat gttgcaaagt
     2101 atctttctaa agaggaccag aagacttacc ttggcttaag tacgagcagt tattccttgt
     2161 cattagttat cggtacatta tcaggtggat ttatttctac gtatttacac tggacaaata
     2221 tgtttttaat tgcattaatc gtagtattta cgttgccatt cctatttaaa ttattaccaa
     2281 aagaaaataa tacgaataaa gctcatttag attttgttgg cttaattcta gtggcaacta
     2341 ttgctacaac agtcatgctg tttattacga actttaattg gttatatatg attggtgcct
     2401 taattgcgat tatcgttttt gcgctatata ttaaaaatgc gcaacgtcca ttagtaaata
     2461 aatcattttt ccaaaataaa cgttatgctt catttttatt tatagtattt gtaatgtatg
     2521 ctatccaatt gggttatatt tttacgttcc cattcataat ggagcaaatt tatcatctgc
     2581 aactagacac aacatcactg ttattagtac cgggttatat agtagcagtc attgttggtg
     2641 cactaagtgg taaaatcggc gaatatctga attcaaaaca agcgattatc acagcaatta
     2701 ttttaatagc actgagcttg attttacctg catttgcagt aggtaatcac atttcaatct
     2761 tcgtcatttc tatgatattc tttgcaggta gctttgcttt aatgtatgca cctttactta
     2821 acgaagccat taaaacaata gatcttaata tgacaggtgt ggctattggt ttttataatt
     2881 taattattaa tgtggcggta tctgtaggta ttgcgattgc tgcggctcta atcgatttta
     2941 aagcattaaa tttcccaggc aatgatgcat taagttcaca tttcggtatt attttaatta
     3001 ttttaggttt aatgagtatt gtcggattag ttttattcgt cagcttaaat cgttggacac
     3061 aatctgaaaa ataaatagat ataaattcgc gagatatatt cgtatttata gtaaaattaa
     3121 ataaagagat tatataacac gaggagtagt aagtatgaaa tttgagaaat atatagatca
     3181 cactttattg aagcctgagt caacacgtac gcaaa
//
LOCUS       DAMQYT010000485         3167 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.guided.238, whole genome shotgun sequence.
ACCESSION   DAMQYT010000485 DAMQYT010000000
VERSION     DAMQYT010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.guided.238"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            122..757
                     /locus_tag="PGA33_002980"
                     /pseudo
     CDS             122..757
                     /locus_tag="PGA33_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            754..1284
                     /gene="sat4"
                     /locus_tag="PGA33_002981"
     CDS             754..1284
                     /gene="sat4"
                     /locus_tag="PGA33_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HDG6474693.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFEKAVFWYLRF"
     gene            1377..2171
                     /locus_tag="PGA33_002982"
                     /note="aph(3')-IIIa"
     CDS             1377..2171
                     /locus_tag="PGA33_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDG6474694.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2447..3018
                     /locus_tag="PGA33_002983"
                     /pseudo
     CDS             2447..3018
                     /locus_tag="PGA33_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         1065 a          497 c          722 g          883 t
ORIGIN      
        1 attagtgatt agctatattt tttactttgc aacagaacca tcatttttat taaaatactc
       61 ttttgtaaaa tctttctgct cttcaatgta tttaatttct ttacctctaa atctattata
      121 gctatttgat gattacaata aaattgatct taccttattg cccttggaag agttagataa
      181 ttacctaaag ggcgataaat taataaaggt tctaattgat aaagattgta gaattaaaag
      241 ggacatagtt ccgactgata tagattatca tgtaagaaag ccaagcgcaa gggagtatga
      301 tgattgctgc aatgaatttt ggaatgtaac accttatgtt attaaaggat tgtgccgtaa
      361 ggaaatttta tttgctattg atcattttaa tcagattgtt cgccatgagc tgctgagaat
      421 gatatcatgg aaggtcggca tcgaaacagg ctttaaatga agtgtaggca agaactataa
      481 gtttattgaa aggtatatat ccgaggattt gtgggagaaa cttttgtcca cctaccggat
      541 ggattcctat gaaaacatat gggaagcatt atttctatgc catcaattgt tcagggcggt
      601 atccggtgag gtggcggaaa ggcttcatta tgcctatccg gagtatgata ggaatataac
      661 aaaatatacc agggacatgt ataaaaaata cactggtaaa accggctgcc tggatagcac
      721 atatgccgct gatatagaag agaggcggga acagtgatta cagaaatgaa agcagggcac
      781 ctgaaagata tcgataaacc cagcgaacca tttgaggtga taggtaagat tataccgagg
      841 tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata tttaaaaagc
      901 taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata tattgacaat
      961 actgataaga taatatatct ttactaccaa gacgataaat gcgtcggaaa agttaaactg
     1021 cgaaaaaatt ggaaccggta cgcttatata gaagatatcg ccgtatgtaa ggatttcagg
     1081 gggcaaggca taggcagcgc gcttatcaat atatctatag aatgggcaaa gcataaaaac
     1141 ttgcatggac taatgcttga aacccaggac aataacctta tagcttgtaa attctatcat
     1201 aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaaagct
     1261 gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta
     1321 taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg
     1381 ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag
     1441 atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat
     1501 atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg
     1561 acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc
     1621 atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt
     1681 atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct
     1741 ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg
     1801 aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag
     1861 acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag
     1921 aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca
     1981 aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg
     2041 ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt
     2101 ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg
     2161 aattgtttta gtacctagat ttagatgtct aaaaagcttt aactacaagc tttttagaca
     2221 tctaatcttt tctgaagtac atccgcaact gtccatactc tgatgtttta tatcttttct
     2281 aaaagttcgc tagatagagt tctatattag agatgtaaag agttttggtg aagaggtttg
     2341 tgagtatggc ttttataacc agggtgcaat gagaagcact aacagcagct agcttaagaa
     2401 cgcataggat atacttttta tggaagtcac agaggaggaa taaaatttga acaggattaa
     2461 cagagtaacc tctattctta ttcagctgca atcaaaaaaa taattcctgc caaagaaatt
     2521 gcacagcgat ttaatataag cttaaggaca gtttacagag atatccggac acttgaagaa
     2581 gccggaatac caattggatc tgaggccgga aaaggatatt ttcttgtaga gggcttccta
     2641 cttctgccgg taatgtttac agcggcggaa gtgggtgcat tgattacagc agggaaattt
     2701 ttaaattgcc atggagatga atcatttata aaggattttg attcagctat gtataaaatc
     2761 aaatctattt tgaagcatgg tgaaaaaaac tatgcacagg agttggagaa cagtattaac
     2821 gtgtacagca catctggaca gaaaaataca ttggcggata acgttattgc agcaattcag
     2881 actgcaatct gcaataaaag ggtaatatca attcaatatc ctgcatcagg agggcaagaa
     2941 ccggaaagca gaatgataga gcctatatca ctggggtttt atgaacagaa ctggtactta
     3001 atcggttttg caggctaaga aatgaatata ggaattttcg tgtggatcgg attaaaagca
     3061 tttgcataga agaagagctc tgtcaaagcc atgatggctc tttggaacaa atattaaaac
     3121 aaatgctggt tctgttgcaa agtaaaaaaa tatagctaac cactaat
//
LOCUS       DAMQYT010000486         2172 bp    DNA     linear   BCT 14-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate NICU_A_t008_28
            SAMN05728397-rid16696493.guided.284, whole genome shotgun sequence.
ACCESSION   DAMQYT010000486 DAMQYT010000000
VERSION     DAMQYT010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05728397
            Sequence Read Archive: SRR4139980
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001572244.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001572244.1
            Genome Coverage       :: 78x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/11/2023 01:36:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,987
            CDSs (total)                      :: 2,922
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 35 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 13 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05728397-rid16696493.guided.284"
                     /serotype="t008"
                     /isolate="NICU_A_t008_28"
                     /isolation_source="nares or umbillicus"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: Jacksonville, FL"
                     /collection_date="2009"
                     /collected_by="University of Florida Emerging Pathogens
                     Institute"
     gene            1..480
                     /locus_tag="PGA33_002984"
     CDS             1..480
                     /locus_tag="PGA33_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDG6474695.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            495..1334
                     /gene="fakB2"
                     /locus_tag="PGA33_002985"
     CDS             495..1334
                     /gene="fakB2"
                     /locus_tag="PGA33_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HDG6474696.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            1420..1953
                     /gene="msrA"
                     /locus_tag="PGA33_002986"
     CDS             1420..1953
                     /gene="msrA"
                     /locus_tag="PGA33_002986"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDG6474697.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            1946..>2172
                     /locus_tag="PGA33_002987"
     CDS             1946..>2172
                     /locus_tag="PGA33_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HDG6474698.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIE"
BASE COUNT          791 a          332 c          386 g          663 t
ORIGIN      
        1 atgactttat ccattctagt tgcacatgac ttgcaacgag taattggttt tgaaaatcaa
       61 ttaccttggc acctaccaaa tgatttgaag catgttaaaa aattatcaac aggtcatact
      121 ttagtaatgg gtcgtaagac atttgaatcg attggtaaac cactaccgaa tcgtcgaaat
      181 gttgtactta cttcagatac aagtttcaac gtagagggcg ttgatgtaat tcactctatt
      241 gaagatattt accaactacc gggccatgtt ttcatatttg gagggcaaac attatttgaa
      301 gaaatgattg ataaagtgga cgacatgtat attactgtta ttgaaggtaa attccgtggt
      361 gatacgttct ttccacctta tacatttgaa gactgggaag ttgcctcttc agttgaaggt
      421 aaactagatg agaaaaatac aattccacat acctttctac atttaattcg taaaaaataa
      481 gggggaaaac gaccatgaca aaacagatta tagtaacaga ctcaacatcc gatttatcta
      541 aagaatactt agaagcaaac aacattcatg taattccttt aagtttaact attgaaggag
      601 cttcatacgt tgaccaagta gatattacat cagaagaatt tattaatcat attgaaaatg
      661 atgaagatgt aaagacaagt cagccagcca taggtgaatt tatatctgct tatgaagaac
      721 taggaaaaga tggctctgaa atcataagta ttcatctttc ttcaggatta agtggtacat
      781 ataacactgc ttaccaagca agtcaaatgg tagatgctaa tgtaactgtt attgattcaa
      841 aatctatttc ttttggttta gggtatcaaa tacaacacct agtagagctt gtaaaagaag
      901 gtgtctcaac ttctgaaata gttaaaaagt taaatcattt aagagaaaac attaaattat
      961 ttgtagttat agggcaattg aatcaattaa ttaaaggtgg cagaattagt aaaacaaaag
     1021 gtttgattgg taatcttatg aaaattaaac caattggtac actagatgat ggtcgcttag
     1081 agcttgtgca caatgcgaga actcaaaatt ctagtatcca atacttgaaa aaggaaattg
     1141 ctgaatttat aggagatcat gaaatcaaat ccattggtgt cgcacatgct aacgtcattg
     1201 aatatgttga taaattgaag aaagttttta atgaagcttt tcatgtgaat aattacgata
     1261 taaatgtaac tacaccagtt atttctgcac atactggtca aggtgcgatt ggcctcgtag
     1321 tccttaagaa gtaaatttaa tcttttcagt gttaattact tccatttcaa tcctttatag
     1381 actaaattta taattagata gatagaggag gtaattcata tgacaaaaga atatgcaaca
     1441 ttagcaggag gatgtttctg gtgcatggtt aaaccattta catcatatcc aggcatcaag
     1501 tcagtcgtat ctggttatag tggcggtcat gttgacaacc caacttatga acaggtatgt
     1561 acgaatcaaa ccggccatgt cgaagcagta caaattacgt ttgatccaga ggttacttcc
     1621 tttgaaaata tattagacat atatttcaaa acatttgacc caactgatga tcaagggcaa
     1681 tttttcgata gaggcgaaag ctatcaacca gtcattttct atcatgatga acatcagaaa
     1741 aaggctgctg agtttaaaaa gcaacaatta aatgaacaag gtattttcaa gaaaccagtg
     1801 attacaccta ttaaaccata taaaaatttc tatccagctg aagactacca tcaagattat
     1861 tacaaaaaga acccggtaca ttattaccaa tatcaacgtg gttcaggtag aaaagcgttt
     1921 atagaatcac attgggggaa tcaaaatgct taaaaaagat aaaagtgaac taacagatat
     1981 agaatatatt gttacacaag aaaacggcac tgaaccacca tttatgaatg aatattggaa
     2041 tcattttgct aaaggaattt atgtagataa aatttctggt aaacctttat ttacatctga
     2101 agaaaagttt cattctgaat gtggatggcc tagcttttcc aaagcgcttg atgacgatga
     2161 aattatagaa tt
//
